LOCUS       DATRUK010000001        62244 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.001, whole genome shotgun sequence.
ACCESSION   DATRUK010000001 DATRUK010000000
VERSION     DATRUK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 62244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62244
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.001"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..364
                     /locus_tag="VBE34_000001"
     CDS             <1..364
                     /locus_tag="VBE34_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7262763.1"
                     /translation="KVQGVKATMVDINTRALELAKKNATRNGVVVEVFQSNIYENISK
                     TFDYIISNPPIRAGKQVVHSIIEESICYLNTGGSLTIVIQKKQGAPSAKAKMLDTFGN
                     CDILKKDKGYYILRSEKV"
     gene            690..1079
                     /locus_tag="VBE34_000002"
     CDS             690..1079
                     /locus_tag="VBE34_000002"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEO7262764.1"
                     /translation="MGNIELKKLAVKASENAYVPYSKFPVGAALRTAEGKIFTGCNVE
                     NISYGLANCAERTAIFKAVSEGYKDFSEIAIYGNTEEPISPCGACRQVMVEFFNKNAK
                     VTLIAKNGKTVETTVGELLPYSFVDLK"
     gene            1144..2193
                     /locus_tag="VBE34_000003"
     CDS             1144..2193
                     /locus_tag="VBE34_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO7262765.1"
                     /translation="MNKKIAGIGLASIAVLSLAACGHRGASKSGGKSDSLKVAMVTDT
                     GGVDDKSFNQSGWEGMQAWGKKNGLKKGAGFDYFQSASESDYATNLDTAVSSGYKLIF
                     GIGFSLHDAIDKAADNNKDVNYVIVDDVIKGKDNVASVVFADNESAYLAGIAAAKTTK
                     TKTVGFVGGMESEVITRFEKGFEAGVKSVDKSIKIKVDYAGSFGDAAKGKTIAAAQYA
                     SGADIVYQVAGGTGAGVFSEAKSRNESLKEADKVWVLGVDRDQAAEGKYTSKDGKASN
                     FVLASSIKEVGKSVELIATKTSKGKFPGGNVTTYGLKDGGVDIATTNLSDDAVKAIKE
                     AKAKIISGDIKVPSK"
     gene            2338..3873
                     /locus_tag="VBE34_000004"
     CDS             2338..3873
                     /locus_tag="VBE34_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7262766.1"
                     /translation="MTQNVIEMKEITKKFGDFVANDHINLTVEKGEIHALLGENGAGK
                     STLMNMLAGLLEPTDGQIFINGQPVTIDSPSKSSQLGIGMVHQHFMLVEAFTVAENIV
                     LGNETTQNGVLDIKTAAKEIKELSEKYGLSVNPNAKISDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPSEIKELMTIMKSLVKEGKSIILITHKLDEIRAVADKVTVIRRGK
                     SIETVPVAGASSQQLAEMMVGRSVSFRTEKKEANPTDIILSVKDLVVEENRGGVLAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVTSGQIVIKGKDVTKFSSRQITEL
                     SVGHVPEDRHRDGLVLDMTMAENLALQTYYKEPLSHKGILNFAKIKEYARQLMTEFDV
                     RGAGEHVLARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DKGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVKPDQTNKQELGILMAGGKIEKEE
                     RDV"
     gene            3866..4927
                     /locus_tag="VBE34_000005"
     CDS             3866..4927
                     /locus_tag="VBE34_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7262767.1"
                     /translation="MSKKAQKIAVPLISVVLGIILGAIIMLIFGYDPLWGYEGLFQTA
                     FGSIKNIGEIFRAMGPLILIALGFSVASRAGFFNIGLPGQALSGWIAAGWFALSHPDM
                     PRPAMILCTIIIGIVAGGITGAIPGILRAYLGTSEVIVTIMMNYIVLYSGNAIVQRVF
                     PKSIMRTSDSSVYVSANASYQTDWLSSLTNNSRINIGIFIAIIAVVLVWFQLNKTTLG
                     FEIRSVGLNPNASEYAGMSAKRTIILSMIISGAFAGLGGVVEGLGTFENVFVQPSSLA
                     IGFDGMAVSLLAANSPIGILFAAFLFGVLSVGAPGMNIAGIPPELIKVVTASIIFFVG
                     VHYIIEYVIKPKKQMKGGK"
     gene            4929..5885
                     /locus_tag="VBE34_000006"
     CDS             4929..5885
                     /locus_tag="VBE34_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7262768.1"
                     /translation="MSLTTIFALLFSSMLIYATPLIFTSIGGTFSERGGIVNVGLEGI
                     MVIGAFSGVVFNLEFASVFGDATPWISVLVGGLVGLIFSVIHAVATVNFRADHIISGT
                     VLNLMAPSLAVFLIKVLYNKGQTDNIQESFGKFNFPILSDIPFVGDIFFKGTSLVGYI
                     AILFSFLAWFILYKTRFGLRLRSVGEHPQAADTLGINVYLMRYSGVLISGFLGGIGGA
                     VYAQSISVNFAATTILGPGFISLAAMIFGKWNPIGAMLASLFFGLSQSLAVIGSHLPL
                     LSNIPTVYLQIAPYVLTIIVLAAFFGQAVAPKADGINYIKTK"
     gene            6091..7461
                     /locus_tag="VBE34_000007"
     CDS             6091..7461
                     /locus_tag="VBE34_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO7262769.1"
                     /translation="MSKIVVVGTNHAGTAAIKTMLSNYGEANEIVTFDQNSNISFLGC
                     GMALWIGEQIDGPEGLFYSDKEQLESMGAKVYMNSPVLNIDYDKKEVTALVDGKEHVE
                     SYEKLILATGSQPIIPPIKGVEIQEGSREFKATLENLQFVKLYQNSEEVIEKLAKPGI
                     NRVAVVGAGYIGVELAEAFQRIGKEVTLVDVADTCMGGYYDRDFTDMMSKNLEDHGIR
                     LAFGQAVQAVEGDGKVERLVTDKETFDVDMVILAVGFRPNTELGAGKLDTFRNGAWVV
                     DKKQETSVKDVYAIGDCATIWDNSRDDINYIALASNAVRTGIVAAHNACGTELEGAGV
                     QGSNGISIYGLNMVSTGLTLEKAKQAGYNAVETGFNDLQKPEFIKHNNHEVAIKIVYD
                     KDSRVILGCQMVSHEDVSMGIHMFSLAIQEKVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(7582..8571)
                     /locus_tag="VBE34_000008"
     CDS             complement(7582..8571)
                     /locus_tag="VBE34_000008"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO7262770.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPALF
                     DKAVGDAEDLSHALAFTSPKKIYAATYADCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALADKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVQLEQWLQENRDIDEQGLVDLF
                     ISVRDAAYSIINKKGATYYGIAVALARITKAILDDENAVLPLSVYQEGQYGDVKDVFI
                     GQPAIVGAHGIVRPVNIPLNDAELQKMQASAEQLKDIIDEAWKNPEFQEASKN"
     gene            8810..11269
                     /gene="gyrA"
                     /locus_tag="VBE34_000009"
     CDS             8810..11269
                     /gene="gyrA"
                     /locus_tag="VBE34_000009"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEO7262771.1"
                     /translation="MQDKNLVDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVDFQDNYDGSEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGESIDAVKLVMDNPDVTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYETGKGSIVLRSRTEIETTSNGKERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRIEGITAVRDESSREGVRFVIEVRRDASANVILNNLFKLTSLQTNFSFNMLAI
                     EKGVPKILSLRQIIDNYIEHQKEVIVRRTQFDKAKAEARAHILEGLLVALDHLDEVIT
                     IIRNSETDTIAQAELMSRFELSERQSQAILDMRLRRLTGLERDKIQSEYNDLLALIAD
                     LADILAKPERVVTIIKEEMDEVKRKYADARRTELMIGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGIQGTGVNNDDFVRELVSTSTHDTVLFFTNLGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLDEGETIQTIINARKEDVANKYFFFTTQQGIVKRTS
                     VSEFSNIRQNGLRAINLKENDELINVLLIDENEDVIIGTRTGYSVRFKVNAVRNMGRT
                     ATGVRGVNLREGDKVVGASRIVNGQEVLIITEKGYGKRTEASEYPTKGRGGKGIKTAN
                     ITAKNGPLARLVTINGNEDIMVITDTGVIIRTNVANISQTGRSTMGVKVMRLDQEAKI
                     VTVALVEQEIEDKSNIEDTKE"
     gene            11276..12019
                     /locus_tag="VBE34_000010"
     CDS             11276..12019
                     /locus_tag="VBE34_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEO7262772.1"
                     /translation="MRNKKKSHGFFNFVRWLLVVLLIIVGLALVFNKPIRNAFIAHQS
                     NHYQISRVSKKTIEKNKKSKTSYDFSSVKSISTESILSAQTKSHNLPVIGGIAIPDVE
                     INLPIFKGLGNTELSYGAGTMKENQIMGGPNNYALASHHVFGLTGSSKMLFSPLEHAK
                     KGMKVYLTDKSKVYTYTITEISKVTPEHVEVIDDTPGKSQLTLVTCTDPEATERIIVH
                     AELEKTGEFSTADESILKAFSKKYNQINL"
     gene            12035..12448
                     /locus_tag="VBE34_000011"
     CDS             12035..12448
                     /locus_tag="VBE34_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7262773.1"
                     /translation="MKLKAVHHIAIIVSDYEKSKDFYVNKLGFEIIRENHRPERHDYK
                     LDLRCGDIELEIFGNRLDDPEYETPPQRIGRPNWPREACGLRHLAFYVPDVEAYKVEL
                     ENLGIFVEPIRYDDYTGKKMTFFFDPDGLPLELHE"
     gene            12601..13563
                     /locus_tag="VBE34_000012"
     CDS             12601..13563
                     /locus_tag="VBE34_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEO7262774.1"
                     /translation="MSIKKLLFAGLAFILFTLASPAYAASDVQKVIDETYVQPDYVLG
                     YSLNQEQRAQTLQLLNYDESRDTKVKTLNTSSYAKIMNIADDASIQLYSSVKIKKLGS
                     NDTLAVNIVTPENITKVTEDMYRNAAVTLGIEHATISVAAPIKVTGESALAGIYYSLE
                     KNGASVSSENKQLAQEELSTLSGINAENKGKEGYDADKLNVALTDIKSAVAKGGSDLS
                     KDDIRKIVEETLKNYHLDNAVTENQINLIVNFAVNLSQSNVIKNSDFTNTLNNLKDNI
                     VSKAGSKFKNINVNFNANKAVESGKGFLANIWQQIVNFFQQFGS"
     gene            complement(13626..14753)
                     /locus_tag="VBE34_000013"
     CDS             complement(13626..14753)
                     /locus_tag="VBE34_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO7262775.1"
                     /translation="MHIIIQITIILLASVLATLISKRIGIPAVVGQLLVGIIIGPAML
                     GLVHQNQVLHVLSEIGVILLMFLAGLEANFDLLKKYLKPSLLVAITGVIVPMALFYFL
                     TRLFGFQINTAIFYGLVFAATSISITVEVLQEYNRVKTDTGAIILGAAVADDVLAVLL
                     LSVFIATNGSSSNIGLQIIIQLLFFVFLFICMKYLVPALFKLIEKVHFFEKYTILAIL
                     ICFSLSILADKVGMSSIIGSFFAGLAIGQTSFVDKVEHKISLLSYTFFIPIFFASIAL
                     PLKFDGMMSHLHTILIFTALAVLSKLIPGYFVGRGFNFSKLESLTIGGGMVSRGEMAL
                     IIVQVGLAAKIISSTTYSELVIVVILSTIIAPFILKYSFKD"
     gene            complement(15023..16585)
                     /gene="guaA"
                     /locus_tag="VBE34_000014"
     CDS             complement(15023..16585)
                     /gene="guaA"
                     /locus_tag="VBE34_000014"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEO7262776.1"
                     /translation="MTDISILNDIQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TADEIRDINPIGIVLSGGPNSVYADGAFGIDEEIFELGIPILGICYGMQLITHKLGGK
                     VLPAGEAGHREYGQSALRLRSESALFAGTPQEQLVLMSHGDAVTEIPEGFHLVGDSVD
                     CPFAAMENTEKQFYGIQFHPEVRHSVYGNDILKNFAVNICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQRAIGDQLTCIFVDHGLLRKNEGDQVMDMLG
                     GKFGLNIIRVDASKRFLDLLSGVEDPERKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGTALGMPDEV
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            16798..17496
                     /locus_tag="VBE34_000015"
     CDS             16798..17496
                     /locus_tag="VBE34_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7262777.1"
                     /translation="MLPAYIKIHDAIKKEIDKGTWKIGQRLPSERDLADDYSVSRMTL
                     RQSITLLVEEGILERRVGSGTYVASHRVQEKMRGTTSFTEIVNSQGRKPSSKLISFQR
                     KLANETEIQKLNLSQSDYVVRMERVRYADKVPLVYEVASIPENLIKGFEQSEVTEHFF
                     KTLTENGYEIGKSQQTIYARNASERVASHLEVNAGHAILALTQVSYFTDGKPFEYVHG
                     QYVGDRFEFYLENN"
     gene            17610..18944
                     /gene="trmFO"
                     /locus_tag="VBE34_000016"
     CDS             17610..18944
                     /gene="trmFO"
                     /locus_tag="VBE34_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEO7262778.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEAHRVPAGGAMAVDREGY
                     SEAVTEEIHKHPLIEVIRDEITDIPGDAITVIATGPLTSDSLAAKIHELNGGDGFYFY
                     DAAAPIVDKNTIDMNKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPEDYKGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLNQT
                     FATRKNPNLFFAGQMTGVEGYVESAASGLVAGINAVRRFNGESEVVFPQTTAIGALPH
                     YITHTDSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIATRALKDLEKFLNY"
     gene            19022..19765
                     /locus_tag="VBE34_000017"
     CDS             19022..19765
                     /locus_tag="VBE34_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7262779.1"
                     /translation="MHYKELLESSEKAYLETNRYKHFKNIITSERYFSNYISYKIMPT
                     VSEFLQDMIYLADEQKDYQSDFAFVFFEENAELSSELQRTLSQQGFESEKHIIFTNSL
                     NHLKLFEKEFDDITFTKLNENYLEKYLKFKYKQSIVYGEIYAKQMYNDNRVNLLNNNA
                     KVYLAIKDNQIIGDVTAWDYGEYVEIDDFYVQESYRGQGIGTRLQFDASRGFKKVILI
                     SEEENRKMYEHQGYEEVAYYWTALRSPRK"
     gene            19898..20746
                     /locus_tag="VBE34_000018"
     CDS             19898..20746
                     /locus_tag="VBE34_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO7262780.1"
                     /translation="MKIKKLLGLTTTVVISALILGACGQSKNEDAKVVRVGTMVKSKT
                     EKARWDKIEELVKKKGVKLKFTEFTDYTQPNKALESDEIDINAFQHYNYLNNWNKANK
                     TNLVSVAETYFTSFRLYSGTKNGKGKYQTVSEIPNKATITIPNDAVNESRSLYLLQSA
                     GLLKLKVSGDTLATMSDVVSNPKSLDLKEVDAAQTARSLDSTDAAVINNDFVTEAGIN
                     PKSAIFIEPKSKNAKQWYNLLVAQKGWQDKSKAKAIKEVVKAYHTDAVKKVIEKTSQG
                     LDQPVW"
     gene            complement(20778..21265)
                     /locus_tag="VBE34_000019"
                     /pseudo
     CDS             complement(20778..21265)
                     /locus_tag="VBE34_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140790.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
     gene            21431..22393
                     /locus_tag="VBE34_000020"
     CDS             21431..22393
                     /locus_tag="VBE34_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEO7262781.1"
                     /translation="MRRKIVLLFVVPMLIVLGILGVVVHYYGSALNIYLLPPSSERYG
                     RVILDRVEQRGLYSQGRQWQIIRQRSEKKLKTSKSYQESRNIVQEAVRYGGGKHSQIL
                     SKETVRRDTLDSRYPEYRRLNEDILLITIPSISKLDKRSISHYSGKLQNILMEKSYKG
                     LILDLSNNTGGNMIPMIGGLASILPNDTLFHYTDKYGNKKTITMKNIPLEALKISRKT
                     INTKHVPIAIITNHKTASSAEMTFLSFKGLPNVKSFGQATAGYTTVNETFMLYDGARL
                     ALTTGIVSDRQGYKYENTPILPDQVTSLPLQESQSWLKSRINQN"
     gene            22419..23171
                     /locus_tag="VBE34_000021"
     CDS             22419..23171
                     /locus_tag="VBE34_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7262782.1"
                     /translation="MLLEINHLEKVFRTRFSKEETRALQDVDFKVEQGEFIAIMGESG
                     SGKTTLLNILATLEKPTNGQVILNGEDITKIKEAKLASFRLKNLGFVFQDFNLLDTLS
                     VRDNIYLPLVLDRKRYKEMDHRLSELSSHLRIDDLLDKRPFELSGGQKQRVAIARSLI
                     TNPQILLADEPTAALDYRNSEDLLNLFETINLDGQTILMVTHSANAASHAKRVLFIKD
                     GRIFHQLYRGNKNNSEFNKDISLTMSAILGGE"
     gene            23173..25128
                     /locus_tag="VBE34_000022"
     CDS             23173..25128
                     /locus_tag="VBE34_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7262783.1"
                     /translation="MFYLKIAWHNLKHSIDQYIPFLLASLLLYSLTCSTLLILMSAVG
                     RDMGTAATVLFLGVIVLSIFAVVMEHYSYNILMKQRSSEFGLYNILGMNKRQVARVAS
                     LELFIIYIFLISIGSLFSAFFAKFIYLIFVNIINYHALNLSLSLWPFIICIVIFTGIF
                     LTLEVPVIRHVHLSSPLSLFRKKQQGEKEPKGNLILAILALVAIAIAYTMALTSGKAP
                     ALAVIYRFFFAVLLVIAGTYLFYISFMTWYLKRLRQNKHYYYKSEHFVSTSQMIFRMK
                     QNAVGLASITLLAVMALVTIATTVSLYSNTQNVVTGLFPKSVSLSIDNSKGDAKNIFE
                     EKILKKLGKSSKEAITYNQTMISMPVSQSSELNITSKNVKHVDITKTGFMYLITQNDF
                     RRLGHQLPKLKDNQVAYFVQKGDSRLKKINLLGNKFDVVKNLKEAYVPETTNTYNPGL
                     IIFANNKQIDNIRKAYLPYTKNINTFPKTFKAYLDLNSQEINSISKNDIIEVDGKYVG
                     NISTKQSFLKEGYQMFGGLLFTGFLLGISFLLGIALIVYYKQYSEGHEDKRSYRILQE
                     VGMSKKLVKRTINSQIMIFFFQPLVVAVIHFGVAIPMLKQMLLVFGVLNSTIVYVVSG
                     LTVLAISIIYFIIYRITSRTYYHIIER"
     gene            25238..25906
                     /locus_tag="VBE34_000023"
     CDS             25238..25906
                     /locus_tag="VBE34_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7262784.1"
                     /translation="MSQEQGKIYIVEDDMTIVSLLKDHLSASYHVSSVSNFRDVKQEI
                     IAFQPDLILMDITLPYFNGFYWTAELRKFLTIPIIFISSSNDEMDMVMALNMGGDDFI
                     SKPFSLAVLDAKLTAILRRSQQFIQQELTFGGFTLTREGLLSSQDKEVILSPTENKIL
                     SILLMHPKQVVSKESLLEKLWENDSFIDQNTLNVNMTRLRKKIVPIGFDYIHTVRGVG
                     YLLQ"
     gene            25903..26841
                     /locus_tag="VBE34_000024"
     CDS             25903..26841
                     /locus_tag="VBE34_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7262785.1"
                     /translation="MIRQFLREHLIWYILYIMMFVLFFISFYLYHLPMPYLFNSLGLN
                     VIVLLGISIWQYSRYRKKMLHLKYFNSSQDPSFELQPSDYAYFNIITQLEAREAQKVS
                     ETIEQTNHVALMIKMWSHQMKVPLAAISLMAQTNHLDPKEVEQQLLKLQHYLETLLAF
                     LKFRQYRDDFRFEAVSLREVVVEIIKSYKVICLSKSLSIIIEGDNIWKTDKKWLTFAL
                     SQVLDNAIKYSNPESKIIISIGEESIRIQDYGIGILEEDIPRLFEDGFTGYNGHEHQK
                     ATGMGLYMTKEVLSSLNLSISVDSKINYGTAVSIHK"
     gene            complement(26884..27954)
                     /gene="xerS"
                     /locus_tag="VBE34_000025"
     CDS             complement(26884..27954)
                     /gene="xerS"
                     /locus_tag="VBE34_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEO7262786.1"
                     /translation="MKRELLLEKIDELKEIMPWYVLEYYQSKLSVPYSFTTLYEYLKE
                     YRRFLEWLLDSGVANCHHIAEIELSVLENLTKKDMEAFILYLRERPLLNANTRQNGVS
                     QTTINRTLSALSSLFKYLTEEVENADGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGNETIEFLEYIDCEYQNKLSKRALAFFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LKDINLNVMVIDVTRKGGKRDSVNVASFAKPYLANYLDIRKNRYKAENQDIALFLSEY
                     RGVPNRIDSSSVEKMVAKYSQDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDKL"
     gene            28339..29934
                     /locus_tag="VBE34_000026"
     CDS             28339..29934
                     /locus_tag="VBE34_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7262787.1"
                     /translation="MSSFNRKKLKFLGISLATLTATTVTLVACGNESKNSGDNKVINW
                     YIPTEISTLDISKNTDAYSNLAIGNSGSNLLRIDKEGKPKPDLAKKVSVSSDGLTYTA
                     TLRDNLKWSDGSKLSAEDFVYTWRRIVDPKTASEYAYLATESHLLNADKINSGDIKDL
                     NKLGVTAKGNQVTFKLTSPCPQFKYYLAFSNFMPQKQSYVEKVGKDYGTTSKNQIYSG
                     PYLVKDWNGSNGKFKLVKNKYYWDSKHVKTNSVIVQTIKKPDTAVQMYKQGQIDFAEI
                     SGTSAIYNQTGSVKALTNQKIRQALNLATDRKGVVKAAVDTGSTPAESLVPKKLAKLP
                     NGEDLSKYTAPGYTYNTSKAQKLFKEGLAEVGQSSLKLTITADSDSPAAKNAVDYVKS
                     TWESALPGLTVEEKFVTFKQRLEDAKNENFDVVLFSWGGDYPEGSTFYGLFTTNSAYN
                     YGKFSSKEYDNAYQKAITTDALKPGDAANDYKTAEKALFDQSYYNPVYYLGKKGLQNS
                     KLKGLVRNSTGLNVDFTYAYKTE"
     gene            complement(29967..30740)
                     /locus_tag="VBE34_000027"
     CDS             complement(29967..30740)
                     /locus_tag="VBE34_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEO7262788.1"
                     /translation="MISDFLRDNPILTLLFCAHFLADFQWQSQSLADSKSHSWRGLWR
                     HLLIVFLPLAALMILIPETTLLNLSIWGSHIVIDSIKKLSYPWVEEGHFQKAAFIIDQ
                     LAHYTCIIVFYHALPTYLPPNHWLLPIKHFIVIALVFIIITKPINIVFKIFFNKFQAK
                     ELSSLLTQEKTKIMKEKSEDHEETIEGAGAMIGNLERLIMAILLISGQYAAIGLVFTA
                     KSIARYDKISKSQVFAEYYLIGSLFSIISVLITHWVLLV"
     gene            complement(30730..31416)
                     /locus_tag="VBE34_000028"
     CDS             complement(30730..31416)
                     /locus_tag="VBE34_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEO7262789.1"
                     /translation="MYLALIGDIINSKQILERETFQQSFQQLMTELSDVYGEELISPF
                     TITAGDEFQALLKQSKKVFQIIDHIQLALKPVNVRFGLGTGNIITSINSNESIGADGP
                     AYWHARSAINHIHDKNDYGTVQVAICLDDEDQNLELTLNSLISAGDFIKSKWTTNHFQ
                     MLEHLILQDNYQEQFQHQKLAQLENIEPSALTKRLKASGLKIYLRTRTQAADLLVKSC
                     TQTKGGSYDF"
     gene            complement(31483..33048)
                     /gene="ffh"
                     /locus_tag="VBE34_000029"
     CDS             complement(31483..33048)
                     /gene="ffh"
                     /locus_tag="VBE34_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEO7262790.1"
                     /translation="MAFESLTERLQGVFKNIRGKKKLSEKDVQEVTKEIRLALLEADV
                     ALPVVKTFIKHVRERAVGHEIIDTLDPTQQIVKIVNEELTDLLGAETSEIEKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEDNARPMMIAADIYRPAAIDQLKTLGSQINVPV
                     FDMGTNHSAVEIVTKGLEQARENRNDYVLIDTAGRLQIDATLMQELHDVKAIAQPNEI
                     LLVVDSMIGQEAANVAEEFNRQLSISGVVLTKIDGDTRGGAALSVREITGKPIKFTGT
                     GEKITDIETFHPDRMASRILGMGDLLTLIERASQEYDEKRSMELAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMLPGMANNPAMKNFKVDENEIARKRAIVSSMTPEERENPN
                     LLNPSRRRRIAAGSGNTFVDVNKFIKDFNQAKQMMQGVMSGDMNKMMKKMGIDPNNLP
                     KDMPGMDGMDMSNLEGMMGQNGMPDLSSLGGDMDFSQMFGGGLKGKVGAFAAKQSMKR
                     MANKMKKAKKKRK"
     gene            complement(33066..33398)
                     /locus_tag="VBE34_000030"
     CDS             complement(33066..33398)
                     /locus_tag="VBE34_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEO7262791.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     SGVSRQAVYDNIKRTEKILEAYEMKLHMYSDYIVRSQIFDDILEKYTDDAFLQEKISI
                     LSSIDNRD"
     gene            complement(33487..34800)
                     /locus_tag="VBE34_000031"
     CDS             complement(33487..34800)
                     /locus_tag="VBE34_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7262792.1"
                     /translation="MSKPIKISKFKKNVSDSHFIHFFTVFSGIFLVMTVIILQVMRYG
                     VYSSVDSSLKYISTHPKNYINMVMSRTAAYLDNSNIASVKLKPGGQTVANTDIILFTS
                     EEEVINYFDAFSNYQFLKPNKKNLGGISELTLTNIFGQDETYHAVTVKVNNPAYPNVT
                     YMTAIVNIDQLVNAKERYEKIIIFVMTTFWIISIGASIYLAKWAQKPIIENYERQKAF
                     VENASHELRTPLAVLQNRLETLFRKPNATILENSENIASSLDEVRNMRILTTNLLNLA
                     RRDDGIKPELAVIKPTLFDSIFENYDLIAQENGKNFTGHNMIQDSFKTDKTLLKQLMT
                     ILFDNAIKYTDNDGSIDFTISETDKYLFLEIADNGPGISEEDKVRIFDRFYRVDKART
                     RQQGGFGLGLSLAQQIVNSLRGNITVIDNKPRGSIFKIKLPKVKG"
     gene            complement(34784..35464)
                     /locus_tag="VBE34_000032"
     CDS             complement(34784..35464)
                     /locus_tag="VBE34_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7262793.1"
                     /translation="MIKILLIEDDLSLSNSVFDFLDDFADVMQIFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESIDDKGQGFDLGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNDNSLIYGDIRVDMSTNSTFVNQTEVELLGKEFDLLVY
                     FLQNQNVILPKSQIFDRIWGFDSDTTISVVEVYVSKVRKKLKGTLFSENLQTLRSVGY
                     ILKHVETD"
     gene            complement(35626..38175)
                     /locus_tag="VBE34_000033"
     CDS             complement(35626..38175)
                     /locus_tag="VBE34_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEO7262794.1"
                     /translation="MKTVEHFVTKFVPENYNLFLDINRQTKTFSGNVAVSGEALDNNI
                     SFHQKGLTIKSVLLDNQPLDFQLDEDNEAMHIQLHETGSMVLVFEFSGHITDNMTGMY
                     PSYYTVNGIKKEVISTQFESHFAREVFPSIDEPEAKATFDLSLKFDQKEGEIALSNMP
                     EINAEQRQETGLWTFDTTPKMSSYLLAFALGELHGKTTHTKNGTLVGSYATKAHQLNE
                     LDFSLDIVVRVIEFYEDYFGVRYPIPQSLHVALPDFSAGAMENWGLVTYREVYLLVDE
                     NSSVSSRQQVALVVAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIDYIEPKLN
                     IFEDFQTGGLPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRW
                     LGDTDFAAGLKIYFEKHQYQNTIGRDLWNALSQTSGKDVAAFMDSWLEQPGYPVMAAK
                     IEEDELILTQKQFFIGEHEDKSRLWQIPLNSNWEGIPEILTEETVVIPNFSQLAEKNK
                     ENGALRFNTENTAHYITNYQGQLLEHIISDLPLMDNISKLQIVQERHLLAESGMISYS
                     SLIPLVSLLSQETSYLVNSAIKSVIDGLSLFVQEDSQDEFDFKEFVNKLSAFNFNRLG
                     FEKREGEGDDSEMVRHLSLSLALYSDNEHAIEEAHHIFKAHENNIAAIPAAIRLLVLT
                     NEMKHFESKELSHLLLETYSTTTDGNFKRQLASALSHTTDSKTLKKLLSDWKNKDIVK
                     PQDLAMSWYATFLKNSFTQESVWEWAQENWEWIKATLGGDMSFDKFVIYPSSSFKTEE
                     RLEQYKNFFEPQLSDMAISRNISMGIKEISARVLLITKQKEEVINTIKKYI"
     gene            complement(38321..38974)
                     /gene="phoU"
                     /locus_tag="VBE34_000034"
     CDS             complement(38321..38974)
                     /gene="phoU"
                     /locus_tag="VBE34_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO7262795.1"
                     /translation="MLRSKFDEELDKLHNQFYAMGIEAIGQIKKTVRAFVSHDRELAK
                     EVIEDDVTLNNFETKLEKKSLEIIALQQPVSQDLRTVITVLKATSDVERMGDHAAAVA
                     KATIRMKGEERIPAVELEINNMGKAVKNMLEEALTAYINGDDEKAYEVAAMDEIVDDY
                     FRDIQKMVVETIQKHPDVAFAAKEYFQVLMHLERIGDYGKNICEWIVYLKTGKIIEL"
     gene            complement(39008..39766)
                     /gene="pstB"
                     /locus_tag="VBE34_000035"
     CDS             complement(39008..39766)
                     /gene="pstB"
                     /locus_tag="VBE34_000035"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO7262796.1"
                     /translation="MTQPILQVSDLSVYYNKKKALKEVSMDFYPNEITALIGPSGSGK
                     STLLRAINRMGDLNPEVTLTGAVMYNGHNVYSPRTDTVELRKEIGMVFQQPNPFPMSV
                     FENVVYGLRLKGIKDKATLDEAVETSLKGASIWDEVKDRLHDSALGLSGGQQQRVCIA
                     RTLATKPKIILLDEPTSALDPISAGKIEETLHGLKDQYTMLLVTRSMQQASRISDRTG
                     FFLDGNLIEYGNTKEMFMNPKHKETEDYITGKFG"
     gene            complement(39778..40581)
                     /gene="pstB"
                     /locus_tag="VBE34_000036"
     CDS             complement(39778..40581)
                     /gene="pstB"
                     /locus_tag="VBE34_000036"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO7262797.1"
                     /translation="MAEYNWDERHIITFPEENSALTTKDLHVYYGEKEAIKGIDMQFE
                     KNKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGQIMYEGIDVNAQDINVYEMRKH
                     IGMVFQRPNPFAKSIYKNITFAYERAGVKDKKFLDEVVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAIAVKPEILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGDLIEYDKTNNIFQNAKCQSTSDYVSGRFG"
     gene            complement(40593..41480)
                     /gene="pstA"
                     /locus_tag="VBE34_000037"
     CDS             complement(40593..41480)
                     /gene="pstA"
                     /locus_tag="VBE34_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO7262798.1"
                     /translation="MNAKKADKLATTILYSIAAIIVTILASLLIFILVRGLPHVSWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSLGAGIYLSEYAKKGRLTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFQYGFSIISGALALTVFNLPQMTRSVEDSLQNVH
                     HTQREAGLALGISRWETVIYVVVPEALPSIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNVLSVTSPISIFRQAETLAVHIWKVNSEGTIPDATQVSAGSAAVLLVVILIF
                     NLSARSIGKKLHSKLTSSN"
     gene            complement(41470..42387)
                     /gene="pstC"
                     /locus_tag="VBE34_000038"
     CDS             complement(41470..42387)
                     /gene="pstC"
                     /locus_tag="VBE34_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO7262799.1"
                     /translation="MKNQELAKKLTSPSKNSRLEKFGKGITFLSLALIVFIVAMILIF
                     VAQKGLSTFFVDGVKLTDFLFNTKWEPSAKSFGALPMIAGSFIVTILSAIIATPFAIG
                     AAVFMTEISPKYGSKILQPAVELLVGIPSVVYGFIGLQIIVPFVRSIFGGTGFGILSG
                     VCVLFVMILPTVTFMTVDSLRAVPRHYKEASLAMGATRWQTIWRVILNAARPGIFTAI
                     VFGMARAFGEALAIQMVVGNSAILPTSLTTPAATLTSVLTMGIGNTVMGTVQNNVLWS
                     LALVLLIMSLAFNTVIKLITREGKKNYER"
     gene            complement(42433..43299)
                     /locus_tag="VBE34_000039"
     CDS             complement(42433..43299)
                     /locus_tag="VBE34_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEO7262800.1"
                     /translation="MKMKKNIFLLTLLATVLLTLSGCANWIDKGQSITSVGSTALQPL
                     VEAAADEFGKTNLGKTINVQGGGSGTGLSQVQSGAVQIGNSDLFAEEKDGIDAKKLKD
                     HQVAVAGLAVIVNKKVNVKNLTTHQLRDIFAGKIKNWKEVGGQDLDISIINRAASSGS
                     RATFDNTIMGNVAPIQSQEQDSNGMVKSIVSQTPGAISYLAFAYVDKSVGTLKLNGFA
                     PTAKNVTTDNWKLWSYEHMYTKGNETGLTKEFLDYMKSDKVQSSIVQHMGYISINDMK
                     VVKDAEGKVTPK"
     gene            complement(43478..44788)
                     /locus_tag="VBE34_000040"
     CDS             complement(43478..44788)
                     /locus_tag="VBE34_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEO7262801.1"
                     /translation="MKLPNEFIEKYQTILKDEAEAFFDSFEQKPISAYRTNPLKEKQL
                     DFPNAIPSTPWGHYGKISGKSIEHTTGLVYSQEPAAQIVAQIAEPQEGMKVLDLAAAP
                     GGKTTHLLSYLNNTGLLVSNEISNKRSKILVENVERFGARNVIVTNESSQRLAKCFNS
                     FFDLIVFDGPCSGEGMFRKDPQAIQYWHKDYPTECAQLQRDILKEAIKMLAHGGILVY
                     STCTWSPEENEEVVNWLLQEYDYLELVDIPKLNGMVEGINVPQVARMYPHHFQGEGQF
                     VAKLRDTRSKEAQKIKPKAQKINKMQLQLWQQFAQDHLKIDLNGVLDVFGDQLYLLPN
                     GLPDLSKLKIARNGLHLGTFKKNRFEPSFALGMALSEHDLVQSIEIDIEQFEVYVSGN
                     VVKLDKTVPNGWYQILVKGNGLGFAKVTNNTLKNYYPKGLRFQT"
     gene            complement(44857..45621)
                     /locus_tag="VBE34_000041"
     CDS             complement(44857..45621)
                     /locus_tag="VBE34_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEO7262802.1"
                     /translation="MDAKFDFAKQLVYKAGQFIKSEMQNTFDVEEKSRFDDLVTSLDK
                     KTQKLLIQEIIQHYPDDNILAEEDDVRSPIAQGNVWVLDPIDGTVNFIVQKDNFAVML
                     AYYEEGVGQFGIIYDVMADILYSGGGHFDVYANDKKIVPFQECPLERCLLGVNSAMYA
                     ENDCGIAHLASETLGVRIYGGAGISMAKVMQGKLLAYFSYIQPWDYAAAKIMGETLGF
                     TLLTLDGEEPNYSTRQKVMFLPKSKLNLIQSYLTKK"
     gene            complement(45611..45892)
                     /locus_tag="VBE34_000042"
     CDS             complement(45611..45892)
                     /locus_tag="VBE34_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEO7262803.1"
                     /translation="MISSNYSYPLDPSWNTEDITKVLRFLNQVEHAYENSVKVDDLLD
                     SYKEFKKFVKSKAQEKQIDREFQRTSGYSTYQAVKAAQQQAKGFISLGR"
     gene            complement(45885..46298)
                     /locus_tag="VBE34_000043"
     CDS             complement(45885..46298)
                     /locus_tag="VBE34_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEO7262804.1"
                     /translation="MITLFLSPSCTSCRKARAWLSKHEVAFEEHNIITSPLNKEELLQ
                     ILSFTENGTEDIISTRSKVFQKLAIDVDELSTSSLMELISENPSLLRRPIILDKKRMQ
                     IGFNEDEIRAFLPRDYRKQELKQATIRAEIEGKHD"
     gene            complement(46341..47273)
                     /locus_tag="VBE34_000044"
     CDS             complement(46341..47273)
                     /locus_tag="VBE34_000044"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEO7262805.1"
                     /translation="MKIHYINDYKDIQAKEDCVLVLGYFDGLHLGHKALFDKAKKIAT
                     EKNLKIVVLTFNETPRLTFARFQPELLLHLTSPEKRSEKFQEYGVDELYLMNFTSHFS
                     KVSSDLFIKKYIYGLRAKAAVVGFDYKFGHNRTSGDYLARNFKGPVYIIDEISEGGEK
                     ISSTRIRQLITEGNVEKANQLLGYEFSTCGMVVHGDARGRTIGFPTANLAPINRTYLP
                     ADGVYISNVLINGKYYRAMTSIGKNITFGGTELRLEANIFDFDGDIYGETIEIFWLKR
                     IREMVKFNGIDDLVKQLKKDKEIALNWKKDSQTL"
     gene            complement(47286..48170)
                     /gene="truB"
                     /locus_tag="VBE34_000045"
     CDS             complement(47286..48170)
                     /gene="truB"
                     /locus_tag="VBE34_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEO7262806.1"
                     /translation="MITGIINLKKEAGMTSHDAVFKLRKILHTKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTESGKIYEGEITLGYATSTEDSSGEVISRTPLTQSDLSEDVV
                     DHAMKSFTGPITQVPPMYSAVKVNGKKLYEYARSGEEVERPKRQITISEFRRTSPLYF
                     EKGICRFSFYVSCSKGTYVRTLAVDLGIKLGYASHMSFLKRTSSAGLSITQSLTLEEI
                     NEKYKQEDFSFLLPIEYGVLDLPKVNLTEEDKVEISYGRRILLENEADTLAAFYENRV
                     IAILEKRGNEFKPHKVLL"
     gene            complement(48262..48693)
                     /locus_tag="VBE34_000046"
     CDS             complement(48262..48693)
                     /locus_tag="VBE34_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7262807.1"
                     /translation="MKIRTATLDDSEKLVPLYQELGYAISLSEIQSILKVILTHSDYG
                     FLIAEDNGKLLAFVGYHKLYFFEKSGTYYRILALVVNEKHRRKGIASQLINHVKQLAK
                     TDGSEVLALNSSLKEYRQEAYHFYENLGFKKVSTGFSYYLD"
     gene            complement(48706..49977)
                     /locus_tag="VBE34_000047"
     CDS             complement(48706..49977)
                     /locus_tag="VBE34_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEO7262808.1"
                     /translation="MNEIKCPHCGTAFAINESEYHQLLEQIRGDAFDKEVSERLEKER
                     LILGEQAKNQLQEVVVEKDKEIAKLQYKVKQFLIEKDNLLKDNEYQLAEQLNQKDMML
                     RDLENQIDRLRLEHENSLQEALTKVERERDAIQNQLHIQEKEKDLALASVKSDYEVQL
                     KAANEQVEFYKNFKAQQSTKAVGESLEHYAETEFNKVRHLAFPNAYFEKDNTLSSRGS
                     KGDFIYREKDENDLEFLSIMFEMKNESDDTIKKHKNEDFFKELDKDRREKSCEYAVLV
                     TMLEADNDYYNTGIVDVSHKYPKMYVIRPQFFIQLIGILRNAALNTLKYKQELALMKE
                     QNIDITHFEEDLDIFKNAFAKNYNSASKNFQKAIDEIDKSIKRMEAVKAALTTSENQL
                     RLANNKLDDVSVKKLTRKNPTMKAKFDALKD"
     gene            complement(50011..50601)
                     /locus_tag="VBE34_000048"
     CDS             complement(50011..50601)
                     /locus_tag="VBE34_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO7262809.1"
                     /translation="MPQNPTSNPEKKTYLLSELVDCFKGKAVPSKAEAGDIRIINLSD
                     MSPLGIDYHNLRTFQDEQRSLLKYLLQEGDVLIASKGTVKKVAIFEEQDYPVVASANI
                     TILRPTQHIRGYYLKLFFDSEEGQQALENANKGKAVMNISTKELLNIAIPSIPLFRQD
                     YLIQRYKQGLNDYKRKIARAEQEWERIQNDIRQQLS"
     gene            complement(50708..51586)
                     /locus_tag="VBE34_000049"
     CDS             complement(50708..51586)
                     /locus_tag="VBE34_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7262810.1"
                     /translation="MRKSIFEETAKGKVSRHVLLTILLALILLFTADFLTYIIIELII
                     SLDFWKLEWDSDIVQLIILLATSISTVMLFMWVKFKEKRPISSLGLFKEGALTELSRG
                     WLVGSVLFGACLILTIISGSGVIETVRFTADKLIWFLIFAVGWQVQSASVEILCRGWL
                     LPVSATKYSKIVSVSISSIFFGLLHSANNHVSLISIFNLCLFGLFLSLYVILKGNIWG
                     ACGIHGAWNCVQGSVFGIEVSGEPMLSNSLVHVKTYGADWISGGKFGVEGSMITSIVL
                     IVACYWLYQKSNFPYD"
     gene            complement(51692..54322)
                     /locus_tag="VBE34_000050"
     CDS             complement(51692..54322)
                     /locus_tag="VBE34_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7262811.1"
                     /translation="MGKTFWKDIYRSITTSKGRFSSILLLMMLGSFAFIGLKVSAPNM
                     QRTAQNYLAHHHVMDITVFNSWGLDKHDQTVLESLKGSQVEFSYFVDTTPQQNSKSYR
                     LYSNTKTISTFDLVKGRLPLNKSEIALSFQERKKYAIGDKINFKQDKNKLFSNTGPLT
                     IVGFVNSTEIWSKTNLGSSQTGDGDLDSYGVLDKTAFHSPVYTMARVTFKDLRLINPF
                     SISYKEKVAKYQEKVSRKLNIHNKIRYTKTKKESLRKIDEEEKSLLKAQKQINRLDND
                     SLAMPLSQRQAIQMKIKQDRLSLLKRTKELLKLRHNTQIMESPQIIVYNRTTFPGGQG
                     YNTFDSSTNSTSKISNLFPIILYLVAALVTLTTMTRFVEEERTNAGILKALGYSDRQV
                     IFKFIIYGFIAGTLGTTLGIIGGHYLLPRIISDIISKDLTIPNTQYHLFLNYSLLAFV
                     FSLLSIVLPVFVITRRELKEKAAFLLLPKPPAKGSKIALEYINWIWKKLSFTQKVTAR
                     NIFRYKQRMIMTIFGVAGSVALLFSGLGIQSSLKQTVNEHFGRIMPYDILLTYNTNAS
                     PPKILELLSKDSKIDKYQPIHLENLDESIPGQINKQSISLFITDKKQLLPFIYLQEAT
                     TNKSLHLNNKGIIISKKLAQFYHVNTGDFIHLSHSQTLPSRKLKITGVVNANVGHYIF
                     MTKQYYRTIFKKEAKDNAFLVKLTKHKIANNLAEKLLEINGVESLTQNALQLASVEAV
                     VRSLDGSMTILVVVSLLLAIVILYNLTNINLAERKRELSTIKVLGFYNEEVTLYIYRE
                     TIILSTIGVILGTISGTYLHRQMMLLIGSDQILFGEKVSPTTFIIPISVVVIILISLG
                     FIVNHQLKKLNMLDALKSVD"
     gene            complement(54334..55035)
                     /locus_tag="VBE34_000051"
     CDS             complement(54334..55035)
                     /locus_tag="VBE34_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7262812.1"
                     /translation="MSYIEMKHSYKRYQTGETEIVANNDISFSIERGELVVILGASGA
                     GKSTVLNILGGMDSNSEGEVLIDGKNIANYTIRELTRYRRYDVGFVFQFYNLVPNLTA
                     LENVELASEIVPKALDAQQALENVGLGHRINHFPAQLSGGEQQRVAIARAIAKKPKLL
                     LCDEPTGALDYQTGKQVLAILQKMAQSKETTVIIVTHNTALAPIANRVIHMHDSKISD
                     IVINENPSDIQNIEY"
     gene            complement(55172..57292)
                     /gene="topA"
                     /locus_tag="VBE34_000052"
     CDS             complement(55172..57292)
                     /gene="topA"
                     /locus_tag="VBE34_000052"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEO7262813.1"
                     /translation="MATTTKTSTKKTSKKKSATAKKNLVIVESPAKAKTIEKYLGRNY
                     KVVASVGHIRDLKKSSMSIDFENNYEPQYINIRGKGPLINDLKKEAKKAKKVYLASDP
                     DREGEAISWHLAHILDLDKEDRNRVVFNEITKDAVKNAFVEPRQINMDLVDAQQARRV
                     LDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENEIKAFQPEEYWTIDGSFKK
                     GTRKFNATFYGLDGKKFKLSNNEDVKTVLKRIKTDEFLVEKVEKKERRRNAPLPYTTS
                     SLQQDAANKINFRTRKTMMIAQQLYEGLSLGTAGHQGLITYMRTDSTRISPLAQNEAT
                     EFITNRFGANYSKHGNKVKNASGAQDAHEAIRPSSVNHTPESIAKYLDKDQLKLYTLI
                     WNRFIASQMTAAVFDTMKVNLTQNGVTFIANGSQVKFDGYMAVYNDTDKNKMLPDMEE
                     GESVKKVNTNPEQHFTQPPARFSEASLIKTLEENGVGRPSTYAPTLETIQKRYYVKLA
                     AKRFEPTELGEIVNSLIVEFFPDIVDVTFTAEMEGKLDEVEIGKEQWQKIIDEFYKPF
                     EKELAKAETEMEKIQIKDEPAGFDCELCGSPMVIKLGRYGKFYACSNFPECHNTKAIT
                     KEIGVICPICQKGQVIERKTKRNRIFYGCDRYPECEFTSWDKPIGRTCPKSNDFLVEK
                     KVRGGGKQVVCSNEKCDYQEEKIK"
     gene            complement(57387..58229)
                     /gene="dprA"
                     /locus_tag="VBE34_000053"
     CDS             complement(57387..58229)
                     /gene="dprA"
                     /locus_tag="VBE34_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEO7262814.1"
                     /translation="MNHFELFKLKKAGLTNLNINNIINYLKKNSLTSLSVRNMAVVSK
                     CKNPTFFIENYKQLDLKKLRQEFKKFPVLSILDSNYPLELKEIYNPPVLLFYQGNIEL
                     LSKPKLAVVGARQASQIGCQSVKKIIKETNNQFVIVSGLARGIDTAAHVSALKNGGSS
                     IAVIGSGLDVYYPTENKKLQEYMSYNHLVLSEYFTGEQPLKFHFPERNRIIAGLCQGI
                     VVAEAKMRSGSLITCERALEEGREVFAIPGNIIDGKSDGCHHLIQEGAKCIISGKDIL
                     SEYQ"
     gene            complement(58367..59395)
                     /locus_tag="VBE34_000054"
     CDS             complement(58367..59395)
                     /locus_tag="VBE34_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7262815.1"
                     /translation="MTKKLIIAILALCTILTTSQAVLAKEKSQTVTIKNNYSVYIKKE
                     KRDKPDNKKQISETLKVPLKPKKVVVFDMGALDTITALGAEKSVIGIPKAKNALSLLP
                     NNVKSVYKAKRYQDVGSLFEPNFEAIARMQPDVVFLGARMASVDNIEKLKEAAPKAAL
                     VYAGVDSKKVFDKGVAERVTMLGKIFDQNKKAKTFNKDIAQAVLKLQKTIEKKGKPTA
                     LFVMANSGELLTQSPSGRFGWIFSVGGFKAVNENEKLSSHGTPVSYEYIAEKNPNYLF
                     VLDRGATIGQGASSKELFNNDVIKATDAVKNKRVHEVDGKDWYINSGGSRVTLRMIKD
                     VQNFVDNR"
     gene            complement(59457..60218)
                     /locus_tag="VBE34_000055"
     CDS             complement(59457..60218)
                     /locus_tag="VBE34_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7262816.1"
                     /translation="MIQINNLHKFYGQKEILKDINISIPKGKVTAILGPNGSGKSTLL
                     SCISRLEPYDNGEIFLDKVPLAHYSSNDLAKTLAILRQSNHLTLKIKVSDLIGFGRFP
                     YSKGRLSQKDKAVIESVISYMDLNDIADEFINNLSGGQIQRAFIAMTMAQDTQYICLD
                     EPLNNLDMKYAVQMMDLIKRYAYEFNKTIVIIIHDINFATHYADNVVALKEGQVVSCG
                     TVEDVMQEKILSHLFDMPIRIETVDGKPIILFCTT"
     gene            complement(60215..61189)
                     /locus_tag="VBE34_000056"
     CDS             complement(60215..61189)
                     /locus_tag="VBE34_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO7262817.1"
                     /translation="MIKDLEIPKLLILLILLIATIILFLIYGIPTDANEFLIIYILKT
                     RYQKLIALILVGICIGSSSLIFQTLTNNRLLTPSIIGLDSLYVLIQTGLMYLIGAQRV
                     IKFSSFSSFLLSLLLMVGFAYLLFTILFRNKKQSLYFVLLAGLIFNTLFSSISSFIQA
                     IMDPNDFMILQNQLFASFNAINTKILWISFIIIVVSFVINWPFIKELDVLLLGKENAI
                     SLGISYQKLTTRIFLWLALMVAIATALVGPITFLGLLVAHITYHSFHTFRHQILVPIA
                     IVICIFTLVLGQHLVQNLLHLTVQLSVLLNLIGGSYFIFTLIKGRKNL"
     gene            complement(61186..62148)
                     /locus_tag="VBE34_000057"
     CDS             complement(61186..62148)
                     /locus_tag="VBE34_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO7262818.1"
                     /translation="MKKLLTTPVMGCTLVILSLTSLFVGVKSIPLEQITHLNQSQVDI
                     FLASRLPRTISILISGASLSVCGLLMQQLTQNKFVSPTTSGTMDWAKLGVVVTLIFFK
                     NTSIFIQLCIASGFAILGSLLFVTILKMITFKDNIFIPLIGLMLGQIVAAATVFLGTH
                     FQVLQSVNSWLQGNFSIMTSHRYEILYLALPCLFLVYFFAHQFTIVGLGESFAKNLGI
                     AYEKMIYFGLVLVSIMTSLVIIIVGALPFLGLIVPNLISLTKGDHMSSTILETSLLGA
                     CIVMICDLFGRLVIFPYEVSIGVTLGVLGSAFFLISIIRNEGKS"
BASE COUNT        20116 a        10521 c        11088 g        20519 t
ORIGIN      
        1 caaagtgcaa ggagttaaag caactatggt tgacattaat acgagagcat tggaattagc
       61 taagaaaaat gcaacacgca atggagtagt agttgaagtc tttcagtcaa acatttatga
      121 aaatatttct aaaactttcg actatattat aagcaaccca cctattcgtg ctggaaagca
      181 agttgttcat tctattatcg aagaaagtat ctgttacctt aatacaggcg gtagccttac
      241 cattgtcatt caaaagaaac aaggcgctcc aagtgcaaaa gctaaaatgc tagatacttt
      301 tggtaattgc gatattttaa aaaaagataa gggatattat atattgagaa gtgaaaaagt
      361 ctaaatgata tccctaaagc gtaaaaataa taaatagttc ggataataac tatcgtatta
      421 ttttttggaa aggttttctc taaaaatgtt ttctttaaat tccgtataag ataaaaagaa
      481 ttctgctttt tattctgaaa attataaaaa actacttaca ttatctaaaa aaaatggtaa
      541 aatagattca gctaaaatac caagactaat gaaactacaa gacttggtta ctcaataata
      601 aatacttctg tgtttgataa gaagataata aacgtgttct aaaaattttg ccagtaaaca
      661 ttatgaagcg aaaggtgcag ctactaaaaa tgggaaatat agagttgaaa aaattggctg
      721 taaaagcgag tgaaaatgca tacgttcctt actcgaaatt tccagtaggc gcagcattgc
      781 gtacagcaga aggaaaaata tttacaggat gtaacgttga aaatatcagt tatggattag
      841 caaactgtgc tgagcgtact gcaattttta aagctgtttc agaaggttat aaagattttt
      901 cggaaattgc tatttatgga aatactgaag aaccaatttc tccttgcggt gcatgccgtc
      961 aagtgatggt ggaatttttt aataagaatg ctaaagttac attgattgct aaaaatggta
     1021 agacggtcga gacgacggtc ggtgagttgt taccatactc gtttgttgat ttaaaataat
     1081 ttaaatcagt tgctctttta agcatttagc ttaaatatta agttttttag gagggttcat
     1141 ttcatgaaca aaaaaattgc cgggatcggc ttggcttcga ttgcagtact tagtttagct
     1201 gcatgtggac atcgtggtgc ttctaaatct ggtggtaaat cagatagctt gaaggttgca
     1261 atggtaacag ataccggtgg tgttgatgat aaatcattta accaatctgg ttgggaaggt
     1321 atgcaagctt ggggcaagaa gaatggcctt aaaaaaggag ctggttttga ctatttccaa
     1381 tcggcaagtg aatctgatta tgcaactaac ttagatacag ctgtgtctag tggttataaa
     1441 ttgattttcg gtattggatt ttctcttcat gatgctattg ataaagcagc agacaataac
     1501 aaagatgtta attacgtcat cgttgatgat gttattaaag ggaaagataa tgttgcaagt
     1561 gttgtctttg cggataatga atcagcttac ttagcaggta ttgcagccgc taaaactacc
     1621 aaaacaaaaa cagttggctt tgtaggtggt atggaatctg aggttattac ccgttttgaa
     1681 aaaggttttg aagcaggtgt caaatcagtt gataaatcaa ttaaaattaa agttgactat
     1741 gctggttcat tcggtgatgc tgctaagggt aagacaattg cagccgcaca atatgcttct
     1801 ggcgcagata ttgtttatca agtagctggt ggaactggtg caggtgtatt cagtgaagca
     1861 aaatcacgca atgaatcttt aaaagaagct gacaaagttt gggtattagg tgtagaccgt
     1921 gaccaagcag cagaaggtaa gtatacttct aaagatggta aggcttctaa ctttgttttg
     1981 gcttcatcaa ttaaagaagt tggaaaatca gttgaattga ttgctactaa aacaagtaaa
     2041 ggtaaattcc caggtggtaa tgtaacaact tatggtttga aggatggtgg agttgatata
     2101 gcaactacaa acctttcaga tgacgccgtt aaagcgatta aagaggctaa agctaaaatt
     2161 atttcaggag atatcaaagt tcctagcaaa taatatcttt aagaactttc aaaaaagcga
     2221 ttagaaatcg cttttttgaa gctgagataa acttatatct cagtaaaatc catcagtcgt
     2281 taacgattgg tggattttac tgaactataa atgaattttt ctgaaaggga gaaccacatg
     2341 acacagaatg tcattgaaat gaaagaaatc actaagaaat tcggtgattt tgtggcaaat
     2401 gatcatatca atttgacggt tgaaaaaggt gagatacatg ctctgcttgg cgaaaacggt
     2461 gcagggaaat caactttaat gaacatgtta gcaggtttac tagaaccaac agacggtcaa
     2521 atttttatta atggtcagcc tgtaactatt gattcacctt ccaaatcgtc acaattgggg
     2581 attggtatgg tacatcagca tttcatgctt gttgaagcat ttactgtagc tgaaaatatt
     2641 gttctaggaa atgaaaccac ccaaaatggt gttttagaca ttaagacagc agctaaagaa
     2701 ataaaggaac tatcagagaa atatggtttg tcagtaaatc caaatgctaa aatatctgat
     2761 atttctgtag gagcacagca gcgtgttgaa attttgaaga cactttatcg cggtgcagat
     2821 attttaattt ttgatgagcc gacggcggtg cttacaccat cagaaataaa agaattgatg
     2881 acaattatga agagccttgt taaagaaggc aaatccatta tactaattac ccataaattg
     2941 gatgaaattc gggcagttgc tgataaagtt actgtgattc gccgtggtaa aagtattgaa
     3001 acagtccctg ttgcaggcgc aagttctcaa caactggcgg aaatgatggt tggacgatct
     3061 gtttccttca gaactgaaaa aaaggaagct aatccgactg acataatcct ttctgtaaag
     3121 gatttagttg tagaagaaaa tagaggcggc gttctagctg ttaaaaatct gtcgttagat
     3181 gttcgagcag gtgaaattgt aggtatagct ggtattgatg gtaatggaca aagtgaatta
     3241 attcaagcta ttacaggttt aagaaaggta acttctggtc aaattgtaat taaaggtaaa
     3301 gatgtcacaa aattttcaag tcgacaaatt acagaattaa gtgtaggtca tgttccagaa
     3361 gatcgtcata gagatggttt ggttctagat atgacgatgg cagaaaattt agctttacaa
     3421 acttattata aagagccatt gagtcataaa ggtatcctta attttgctaa aattaaagag
     3481 tatgctcgtc aattaatgac agaatttgat gtgcgtggtg ctggtgaaca tgttttagca
     3541 agaggatttt ctggtggtaa ccaacaaaaa gctattattg ctcgtgaagt ggatcgcgat
     3601 ccagacctac tcattgttag ccaaccgact cgtggtctag atgttggtgc tatcgagtat
     3661 attcataaac gtttgattga agagcgcgat aaagggaaag cggtcttagt agttagcttt
     3721 gagttagatg aaatcttaaa cttatcagat cgtattgcag ttattcatga tggtaaaatt
     3781 caaggaattg ttaaacccga ccaaaccaac aagcaagaat tgggtatttt gatggctggt
     3841 ggaaaaattg aaaaggagga gagagatgtc taagaaggcg caaaaaattg cagttcctct
     3901 gatttcagtt gtactaggaa tcattctagg agctattatt atgctgatat ttggttatga
     3961 tccactttgg ggttatgagg gattgttcca aacagcattc ggtagtataa aaaatattgg
     4021 tgaaattttc cgtgcaatgg gaccattaat tttaattgct ctaggtttct cagttgccag
     4081 tcgtgcaggt ttctttaata ttggacttcc agggcaagcc ttatcaggat ggattgccgc
     4141 aggttggttc gctctttcac acccagatat gccacgtcca gcaatgattc tttgtactat
     4201 tattattggt atcgttgctg ggggaatcac tggagctatt ccaggtattc tcagagcata
     4261 ccttggaaca agtgaagtta ttgtaactat tatgatgaac tatattgttc tttattcagg
     4321 aaatgctatt gtacaacgtg ttttcccaaa aagcattatg cgtacaagtg actcgagtgt
     4381 atatgtttct gccaatgcat cataccagac agattggtta tctagtttaa cgaataattc
     4441 acgtatcaat attggtatct tcattgctat tattgcagtt gtgttagttt ggttccaatt
     4501 aaataaaaca acgcttggat ttgaaattcg ttcagttggt ttaaatccaa atgctagtga
     4561 gtacgctggt atgtctgcaa agcgtacaat tattctatca atgattattt ctggagcttt
     4621 tgcaggactt ggtggtgttg ttgaaggact aggtacattt gagaatgtat ttgtacagcc
     4681 atcctcgtta gctattggct ttgacggtat ggcagtaagt ctactcgcag cgaatagtcc
     4741 aataggtatt ttatttgcag catttctatt tggtgttcta agtgttggtg cgcctggtat
     4801 gaacattgca ggtattccac ctgagttaat taaagttgta acagcttcaa tcatcttctt
     4861 cgtaggtgtc cattacatta ttgagtatgt tattaaacct aaaaaacaaa tgaaaggtgg
     4921 taagtaaatt gagtttaaca acgatttttg cattgctatt ttcatcaatg ctaatttacg
     4981 caacacctct tatctttaca agtattgggg gaaccttctc tgaacgtggt ggtatcgtca
     5041 acgttggttt agaaggaatt atggtaattg gagctttctc aggcgttgta tttaatttag
     5101 agtttgcttc tgtttttggt gatgcgactc catggatttc tgtactagtt ggtggtttag
     5161 ttggtttaat tttttctgtt atccatgcag ttgcgacagt taattttcgt gcagatcata
     5221 ttatcagcgg aaccgttctt aatttaatgg caccttcatt agctgttttc ttgattaagg
     5281 ttctttataa taaaggtcaa actgataata ttcaagaatc atttggaaaa tttaatttcc
     5341 ctatactatc agatattcct tttgttggtg atatcttctt taaaggaaca agcttagtcg
     5401 gttatattgc tattcttttc tcattccttg cttggtttat tctatataaa actcgctttg
     5461 gattaagatt acgctcagtt ggagaacatc ctcaagcagc agacacctta ggtattaatg
     5521 tttatcttat gcgttactct ggtgtcttga tttcaggttt ccttggtgga ataggaggag
     5581 ctgtatatgc tcaatctatt tctgttaact ttgcagcgac aactatatta ggaccgggct
     5641 ttatctcctt agcggcgatg atttttggta agtggaatcc aattggtgca atgcttgcta
     5701 gtctattctt tggtttatcg caaagtttag cagttatcgg tagccattta ccacttttga
     5761 gcaatattcc aacagtttat ctacaaattg ctccatatgt cctcactatc attgtattag
     5821 cagctttctt tggacaagca gtagctccta aggcggatgg tattaattat attaaaacga
     5881 aataaataaa aagaaactag tataagctag tttcttttta ttgtttgtga atttgtgaaa
     5941 aaaatacttt gtgaaaattt cacataaatt ataatacaat aacaataaaa tataaaaaaa
     6001 tagaaaattg agtcattatt ctagtaagag tttgtgaata atgttataat gtgttttgtt
     6061 attaattatt tataaaggag aactcttaat atgagtaaaa tcgttgtcgt agggacaaac
     6121 cacgctggta cagcagcaat taaaacaatg cttagtaatt atggtgaagc caatgaaatt
     6181 gtaacatttg atcaaaattc caatatctct ttcttaggat gtggtatggc tctttggatt
     6241 ggtgaacaga ttgatggtcc agaaggattg ttctactctg ataaagagca gttggaaagt
     6301 atgggagcta aggtttacat gaactctcca gtacttaata ttgactacga taaaaaagaa
     6361 gttacagctt tagtagatgg aaaagaacat gttgaaagtt atgaaaaact tattttagca
     6421 actggttcac aacctatcat tccaccaatt aaaggtgttg aaattcaaga aggatctcgt
     6481 gaatttaaag ctactcttga aaaccttcaa tttgtgaaac tttatcaaaa ttcagaagaa
     6541 gttattgaaa agttagctaa acctggtatc aatcgtgttg ctgttgttgg ggctgggtat
     6601 attggtgtag aattagcaga agctttccaa cgtattggaa aagaagtaac acttgttgat
     6661 gttgctgata cttgtatggg tggttactat gatcgtgact ttactgatat gatgagtaaa
     6721 aaccttgaag accatggtat tcgtcttgct ttcggacaag ctgttcaagc agttgaaggt
     6781 gatggtaaag tagaacgtct tgttactgat aaagaaacat ttgatgttga tatggtgatc
     6841 ttagctgttg gattccgtcc aaatacagaa ctcggtgcag gtaaattaga tacattccgt
     6901 aatggcgctt gggtagttga taagaagcaa gaaacaagcg tgaaagatgt ttatgctata
     6961 ggtgattgtg cgacaatttg ggataattct cgtgatgata tcaattatat tgctcttgct
     7021 tcaaacgccg ttcgtactgg tattgtagca gctcacaatg catgtggtac agagcttgaa
     7081 ggtgctggtg tccaagggtc aaatggtatc tcaatctatg gccttaacat ggtgtcaact
     7141 ggtttgactt tagaaaaagc aaaacaagct gggtacaatg cagttgaaac aggctttaat
     7201 gaccttcaaa aaccagaatt tatcaaacac aataatcatg aagtagctat taagattgtc
     7261 tatgataaag atagccgagt tatccttggt tgccaaatgg tctcacacga agatgtatct
     7321 atgggaattc atatgttctc acttgctatt caagaaaaag ttactattga aaagcttgct
     7381 ttgaccgata tcttcttctt accacatttt aataagccat ataactatat tacaatggct
     7441 gcattaggcg ctaaagatta atttaaaatt aaaaaggtta agcgaaatgc ttagcctttt
     7501 atttcatcct ttaacaaggt caaaaatgta acggcaaaga aaaagctaag atattcctat
     7561 cctagctttt tttcaactat gttagttttt tgatgcttct tggaattctg ggtttttcca
     7621 agcttcgtca ataatatctt ttaattgttc agctgaggct tgcatttttt gaagttcagc
     7681 atcatttaat gggatattaa ctggacgaac gataccatgt gcacctacga ttgcaggctg
     7741 accgataaag acatctttaa catcaccgta ttggccttct tgatatacag ataatggaag
     7801 aactgcattt tcatcatcaa ggatagcttt agtaatacga gcaagtgcga cagcaatacc
     7861 gtaatatgta gcaccttttt tgttgatgat tgagtatgca gcgtcacgaa ctgaaataaa
     7921 caaatcaaca agtccttgtt catcaatatc gcgattttct tgtaaccatt gttcaagttg
     7981 gacaccagca acgttagcat gtgaccaaac ggcaaattct gagtcaccgt gttcacccat
     8041 gatgtatgcg tgaactgaac gagcatcaac accaatttta tctgctaaag cttgacggaa
     8101 acgtgcagag tcaagtgaag tacctgaacc aataacgcgt tccttaggga aacctgagaa
     8161 tttccaagtt gaatatgtaa gtacatctac agggttagcc gctactaaga agataccgtt
     8221 gaaacctgat tcaacaactt gagttacgat tgatttgtta attgctaagt ttttaccaac
     8281 aaggtcaagt cgagtttcac ctggtttttg aggtgcgcca gcagtaatga caacaaggtc
     8341 agcatctgcg cagtctgcat aagtagctgc gtagattttt ttaggtgatg taaatgcaag
     8401 ggcatgtgaa agatcttcag catcaccaac agctttatca aataaagctg ggatttcaat
     8461 gatacctaac tcttgcgcaa taccttggtt aacaagtgca aaagcgtaag aagaacctac
     8521 ggcaccatca ccaacgagaa taactttttt atgttgttta gttgcagtca tttctaaaca
     8581 tctccttaaa tatttttggg aagtttccca tacacctata ttctatcatt ttcaatgaaa
     8641 gaagtcacta ataaaatgtg aaaaaatttg caaacaaatt gaaatatcat agaaaactat
     8701 tgaaaacgct gagtgtcttg atatttttga taccgattta tcatcgcaat tttacctaaa
     8761 ttatggtaca atgtaagaga cttacagtga agaaaagggg cattttttaa tgcaagataa
     8821 aaatttagta gatgttaatc taactagtga aatgaaaacg agttttatcg attacgccat
     8881 gagtgtcatt gttgctcgtg cacttccaga tgttagagat ggtttaaaac ctgttcatcg
     8941 tcgtatttta tatggtatga atgaactagg tgtgacacct gataaacctc ataagaaatc
     9001 agcacgtatt actggtgatg ttatgggtaa ataccatcca catggtgatt catctattta
     9061 cgaagcaatg gtgcgtatgg cacaatggtg gtcatatcgt catatgctcg ttgatggtca
     9121 tggaaacttt ggttcaatgg atggggatgg tgctgccgca cagcgttata cagaagcacg
     9181 tatgagtaaa attgctcttg agatgcttcg tgatatcaat aaaaatacag ttgattttca
     9241 agataactat gatggcagtg aacgtgaacc tcttgtttta ccagcgcgtt tccctaattt
     9301 attagtcaat ggtgcaactg gtattgctgt aggtatggca acaaatattc caccacataa
     9361 tctaggtgag tctatagatg ctgtcaaatt ggttatggat aaccctgatg ttactacacg
     9421 ggagttaatg gaagttattc ctggtccaga ttttcctact ggtgccttgg tgatgggacg
     9481 ttcaggtatt caccgtgcct atgaaactgg gaagggatca attgtcttac gttcacggac
     9541 tgaaattgaa acaacttcaa atgggaaaga gcgtattgtt gtcacagagt ttccatatgg
     9601 cgttaataaa acaaaagtac acgagcatat tgtacgttta gcacaagaaa agcgcattga
     9661 aggtattact gctgttcgtg atgaatcaag tcgtgaaggc gttcgttttg ttattgaagt
     9721 tcgtcgtgat gcttcagcaa atgtgatttt aaataatctt ttcaaattaa ctagcttgca
     9781 gacaaatttt agcttcaata tgcttgctat tgaaaaaggt gttccaaaaa ttttgtcatt
     9841 acgacaaatc attgataact atattgaaca ccaaaaggaa gttattgttc gtcgtactca
     9901 gtttgacaaa gctaaagcag aagcccgtgc tcatatctta gaaggtttac ttgtggcgct
     9961 tgatcacttg gatgaagtta tcacaattat tcgtaatagt gagacagaca ccattgctca
    10021 agcagagttg atgtcacgtt ttgaattatc ggagcgtcag agtcaagcta ttttagatat
    10081 gcgtcttcgt cgtttaactg gattagaacg cgataaaatc caatcagaat acaatgactt
    10141 actagcttta attgctgatt tagcagatat cttagctaag cctgaacgtg ttgttaccat
    10201 tataaaagaa gagatggatg aggttaagcg taagtatgct gatgcccgtc gtacagaatt
    10261 aatgattggt gaagttttat cacttgaaga tgaagacctt attgaggaag aagatgttct
    10321 tattactctc tcaaacaagg gatatatcaa gcgtctcgct caagatgagt ttagagcaca
    10381 aaaacgtgga ggacgcggta ttcaagggac tggagttaat aacgatgatt ttgttcgtga
    10441 attggtttca acaagtactc acgatactgt gcttttcttt acaaatttag ggcgtgtgta
    10501 tcgtttgaaa gcatatgaaa ttccagaata tggacgtaca gctaaaggcc tccctatcgt
    10561 taacctttta aaacttgacg aaggtgagac aattcaaaca attattaacg ctagaaaaga
    10621 agatgtcgct aataaatatt tctttttcac aactcagcaa ggtatagtga aacgtacaag
    10681 tgtttctgaa tttagtaaca ttcgtcaaaa tggacttcgt gctattaatt tgaaagagaa
    10741 tgacgagttg attaacgtcc tcttgattga cgaaaatgaa gatgttatta tcgggacacg
    10801 tacaggctac tctgttcgct tcaaagtgaa cgctgtacgt aatatgggac gcacagcgac
    10861 tggggtacga ggcgttaatc ttcgtgaagg tgataaagtt gtgggagctt ctcgtatcgt
    10921 taatggacaa gaagtactaa tcatcactga aaaaggttat ggcaagagaa cagaagcttc
    10981 ggaatatcct acaaaaggcc gtggtggtaa aggaataaaa accgctaata ttactgctaa
    11041 aaatgggcca ttagcacgct tagtgacgat taatggaaat gaggacatca tggtcattac
    11101 agatacaggt gttattattc gaaccaatgt tgctaatatt tctcaaactg gtcgttctac
    11161 catgggtgtt aaagtgatga gacttgatca agaagctaaa atcgtcactg ttgcgctagt
    11221 ggaacaagaa attgaagata agtctaatat agaggataca aaagaatagg aagttatgcg
    11281 taataaaaaa aaatcacatg gtttctttaa ctttgttaga tggttattgg ttgtattgct
    11341 aattattgtt gggttggctt tggtgttcaa taaaccaatt cgcaatgctt tcatagctca
    11401 tcaatcaaat cattatcaaa tttcacgagt tagtaaaaaa acaattgaaa aaaataagaa
    11461 atctaaaaca agttatgact tttcatcagt caaatctatt agtacagaat cgattttatc
    11521 tgctcaaacg aaatcacata atttgcctgt aataggaggg attgctatac cagatgtcga
    11581 aatcaatctc ccaattttca aaggactggg caatacagag ttgagttatg gtgcaggaac
    11641 gatgaaagaa aaccagatta tgggaggccc aaataactat gccttagcta gtcaccatgt
    11701 ctttggtttg acaggttctt ctaagatgct cttttcacct ttagaacatg ctaagaaagg
    11761 tatgaaagtt tacctcactg ataaaagcaa agtatacact tacactatta ctgaaatttc
    11821 aaaggtaaca ccagaacatg ttgaggttat tgatgataca cctggcaaga gccaactcac
    11881 tttagtgacc tgtacggatc cagaagccac agaacgtatt attgtgcatg ctgaattaga
    11941 aaaaacaggt gaattttcaa cagcagatga gtcgatatta aaagcatttt cgaaaaaata
    12001 taatcaaatt aatctctaga aggtagttag aattatgaaa ttaaaggctg ttcatcatat
    12061 tgcgataatt gtttctgact atgaaaagtc aaaagacttc tatgttaata agctaggatt
    12121 tgaaattatt cgtgaaaacc atcgtccgga gcgtcatgat tataaattag atttacggtg
    12181 cggtgatatt gagcttgaaa tatttggaaa tcgtcttgat gatccagaat atgaaactcc
    12241 tcctcagaga ataggtaggc cgaattggcc acgtgaagca tgtggtttac gacaccttgc
    12301 cttttacgtt ccagatgtgg aagcgtacaa ggtagaactg gagaatttag gaatttttgt
    12361 agagcctatt cgttacgatg actatactgg caaaaagatg acctttttct ttgatccaga
    12421 tggtttacct ttagaattac atgaataata aacaaagttg cgtggatacg caactttgtt
    12481 taatttactg aataatttta tattcaagaa gttttttaaa gaaataagta agttaatcat
    12541 ggtataatgt aaggtatgaa aagaattgaa aaggaattat tttgaaatga ggatactaaa
    12601 atgagtatta aaaagctatt gtttgcaggc cttgctttta ttttatttac cctagcgagt
    12661 ccagcatatg ctgcatcaga tgttcagaaa gttattgatg aaacttatgt tcaacctgat
    12721 tatgtcttag gttattcgct aaatcaagaa cagcgtgcac agacattaca acttctgaat
    12781 tatgatgaat ctagagacac gaaagttaaa acgctaaata ctagctcata tgctaaaata
    12841 atgaatatag ctgatgatgc aagcatccaa ttatattcat ctgttaaaat aaaaaaatta
    12901 gggtctaacg ataccttagc agtaaacatt gttacacctg agaatattac taaagttaca
    12961 gaagatatgt atcgcaatgc tgcagttaca ttaggtattg agcatgcaac gatatcagtg
    13021 gctgctccaa taaaagtaac tggggagagt gctcttgcag gtatctatta ttcattagag
    13081 aaaaatggag caagtgtatc atcagaaaat aaacaactag cacaagaaga gttaagtact
    13141 ttatctggta ttaatgctga gaataagggt aaggaaggtt atgacgccga caagcttaat
    13201 gtagctttaa cggatattaa gtcagccgta gctaaaggag gatctgatct ttcaaaagat
    13261 gatattcgaa aaattgttga ggaaacattg aaaaattatc atcttgataa cgctgtaacg
    13321 gaaaatcaaa taaatttaat tgttaatttt gcagttaatc tttcacaaag taatgtcatc
    13381 aaaaatagtg atttcacgaa caccttgaat aatttgaaag acaacattgt ttcgaaagca
    13441 ggatctaaat ttaaaaatat caatgtcaat tttaatgcta ataaagctgt tgaatcaggt
    13501 aagggatttt tagctaatat ttggcaacaa attgttaatt ttttccagca gtttggtagt
    13561 taataaaaaa gtctagctta taagctagac tttttttacc ctaaataaat gtttgtgttt
    13621 ttttactaat ctttaaatga atatttaaga ataaatggcg caataatagt tgacagtata
    13681 acaacaataa caagctcgga ataagtagtg cttgaaataa ttttagcagc taatccaact
    13741 tgaactataa tgagagccat ctcgccacga ctgaccattc caccaccaat tgttaaagat
    13801 tctaattttg aaaaattaaa gcctcgaccg acaaagtacc ctggaattaa ttttgataaa
    13861 acagccaaag cagtaaagat taatatcgtg tgaaggtgag acatcattcc atcgaattta
    13921 agaggtagtg caattgaagc gaagaaaatt gggataaaga aggtgtaact aagaagtgaa
    13981 attttatgtt ctaccttatc aacaaaagaa gtttgtccaa tagctaatcc tgcaaaaaac
    14041 gaaccaatga tagaactcat tccaacttta tcggctaaaa ttgatagaga aaaacagata
    14101 aggatagcaa gaatagtata tttctcaaag aaatgaacct tttcgattaa tttgaaaaga
    14161 gcaggtacaa ggtatttcat acaaataaat aagaaaacaa agaataacaa ttgaatgata
    14221 atttgtaatc cgatattaga agagcttcca tttgttgcaa taaagacact cagaagcaaa
    14281 acagctaaga catcatcagc aacagcagca cctaaaataa tagcaccagt atctgttttt
    14341 acacgattgt attcttgtaa aacttcaact gtgatagaaa tactagttgc agcaaatacg
    14401 agcccgtaaa agatagcagt attgatttgg aacccaaaca agcgtgtcaa gaagtagaag
    14461 agagccatag gaacaataac accagtaatt gcgaccagta gactgggctt aagatacttc
    14521 tttaaaagat caaaattagc ttcaagaccc gctaaaaaca tgagtaagat aacaccaatt
    14581 tctgacagga catgtaatac ctgattttga tgaacaagtc ctagcattgc aggtccgatg
    14641 atgataccta caagtagttg tccaacaaca gccggtattc caattctttt tgaaataaga
    14701 gttgcaagaa cgcttgccaa caaaatgatt gtaatttgaa tgataatatg cattttttcc
    14761 tcctaatatt aataaaatga tatcaatcat attgtactga atatttagaa taccattttt
    14821 taagcctaaa atctaggaat atttcttttc tgtctttttc atattattat caagaaaatg
    14881 gaaaaatata ctgaaaaata ttcaatttaa gaataatgaa cttgtaacta tcgaactaca
    14941 taaaaatgat attttaagtg taagtatccc ttattcaaga aataagagta aaaaaactta
    15001 gctacttaaa gtagctaagt ttttattccc actcaacagt tgcaggtggt ttacttgtaa
    15061 tatcataaac aatacggtta acgtgatcaa cttcgttaac aatacgtgtc gaaattttct
    15121 taagtacatc ccaaggaagt tgcgcaaagt cagctgtcat gccatcaatt gaagtaattg
    15181 cacggatagc aattgtatag tcataagtac gaccatctcc cataacaccg acagaacgta
    15241 cgccagtatt aactgtgaaa tactgccata cgtcacgatc tagaccagct ttagcgattt
    15301 cttcacgaag aatagcatct gattcgcgga ctgtttcaag tttttcttcg gtaatttcac
    15361 ccataacgcg aatagcaaga ccaggcccag ggaatggttg acgccataca acttcatcag
    15421 gcattccaag tgcagtacca agagcgcgaa cctcatcttt gaataatgtg ttaagtggct
    15481 caatcaattc aaattgcata tcttctggca atccaccaac attatggtgt gacttgattg
    15541 tttgggctgt ttctgtccca gattcaataa tatctgtgta gagggtacct tgagcaagga
    15601 aatcaacgcc tttaagttta cttgcctcat catcaaaaac gtacacgaac tcatttccga
    15661 tgatttttcg ttttctctcg ggatcttcaa caccagaaag aagatctagg aagcgttttg
    15721 aagcatcaac acggataata ttaagtccaa atttaccacc aagcatatcc ataacttggt
    15781 caccttcatt tttacgtaat aaaccatgat ctacaaaaat acatgttaat tgatcaccga
    15841 tagcacgttg taggagtact ccgacaacag atgaatcaac accgccagat agtccaagaa
    15901 gtactttacg atcgcctaca gtttcacgta tttttgcaat ttccatatca atgaagttat
    15961 ccattgacca atcaccacgt gcaccacaga tattaacagc aaaattttta aggatatcat
    16021 tgccatatac tgagtgacga acttcggggt ggaattggat accataaaat tgtttttcag
    16081 tattttccat tgcagcaaat ggacagtcaa cagagtcacc aacgagatgg aaaccctcag
    16141 ggatttcagt aacagcatca ccatggctca tcaaaacaag ttgttcttga ggagtgccag
    16201 caaagagtgc tgattctgag cgtaggcgaa gtgctgattg accatactca cggtgaccag
    16261 cttctcccgc aggaagaacc ttaccaccga gtttatgggt aatcagttgc ataccataac
    16321 aaattcctaa gattggaatt cctaattcaa aaatttcttc atcgattcca aaagcgccat
    16381 cagcgtatac ggaatttgga ccacctgaaa gtacgatacc aataggatta atatcgcgaa
    16441 tttcatcagc cgtaatttta tgtgatttta actcagaaaa tacaccaaat tcacggatac
    16501 gacgtgcgat aagctggtta tattgactac cgtaatctag aacgataatt ttttggatat
    16561 cattcaatat agaaatatca gtcattgttt ccctttcttt gtagctaatt ttaaaaacag
    16621 ctgtacctat accattttag catattttat ataaattaac atcattaaaa taattgatta
    16681 aacgctttct tatcaagaaa tattgcttat gatttttctg aattatttca cgattttaga
    16741 gttagaaata tatagaagta tggtaaaatg aagtgtagat tctaggagga gagaatattg
    16801 ttaccagcat atattaaaat tcacgacgct attaaaaagg aaatcgataa agggacttgg
    16861 aagattgggc agcggttacc tagcgaaaga gatttagcgg atgattatag tgtaagtcgt
    16921 atgacacttc gccaatcaat tacattgtta gttgaagaag gtattttgga gagacgagtt
    16981 ggtagtggca cctatgttgc tagtcatcgt gttcaagaaa aaatgcgggg aacaactagt
    17041 tttacagaaa ttgtcaattc tcaagggcgt aagccatcca gtaaactaat ctcatttcaa
    17101 agaaaactag ctaatgaaac cgaaattcaa aaattaaatt tatcacagtc agattatgtg
    17161 gttcgtatgg aaagggtacg ttatgctgat aaggtaccgt tggtttatga ggtagcctct
    17221 ataccagaga atttaattaa aggttttgaa caatcagagg ttacagaaca tttctttaaa
    17281 accttaacag agaatggcta cgaaattggg aaaagccaac aaactatata tgctagaaat
    17341 gctagtgaac gtgttgcttc gcatttagaa gttaacgcag gccatgctat tttggcattg
    17401 acacaagttt catactttac tgatggtaag ccttttgaat atgttcatgg gcaatatgtt
    17461 ggagatcgtt ttgagtttta tttagagaat aactagtagt ttaatgaata acgttttttg
    17521 aagaagtagc tatgtatgtt ataatggaaa aataaattat atagaaagat ttgaggaagt
    17581 agttgactac tttcaagagg tattagttca tgtctcaatc ttacattaat gttatcggcg
    17641 ctggtttggc tggttctgaa gcagcttatc aaattgctaa acgtggaata ccagttaagc
    17701 tttatgaaat gcgtggggtt aaatcaaccc ctcaacataa aacagataat tttgcagaat
    17761 tagtatgttc caattcattc cgcggtgatt ctctaacaaa tgcagttggt cttttaaaag
    17821 aagaaatgcg tcgtttggat tctatcatta tgagaaatgg ggaggcacat cgagttccag
    17881 caggtggagc catggccgtt gatagagaag gttattctga agctgttact gaggaaatcc
    17941 ataaacatcc attaatcgaa gtaattcgtg atgaaatcac agatatccct ggagatgcta
    18001 ttacagttat cgctacagga ccattaacaa gtgatagtct ggcagctaaa atccatgaac
    18061 ttaatggtgg tgatggtttt tatttttacg atgccgccgc tccaattgtc gataaaaata
    18121 cgattgacat gaataaagtt taccttaagt ctaggtatga caaaggtgaa gcagcttatc
    18181 taaattgtcc aatgaccaaa gaagaattta tggcttttca tgaagcttta actacagctg
    18241 aggaagcacc actaaattct tttgagaaag aaaaatactt cgaaggttgt atgcctatcg
    18301 aagtgatggc taagcgtggt attaaaacga tgctttatgg tccaatgaaa ccagttggtt
    18361 tggagtatcc tgaggactac aaaggaccgc gagatggtga atttaaaaca ccatatgctg
    18421 ttgttcaatt acgtcaagat aatgctgcag gctctcttta taacatagtt ggtttccaaa
    18481 ctcatcttaa gtggggagaa caaaaacgtg tatttcaaat gattcctggt cttgaaaatg
    18541 ctgaatttgt ccggtatggt gttatgcatc gcaattctta tatggattca ccaaatcttt
    18601 taaaccaaac ctttgctaca cgtaagaacc caaatctttt ctttgctgga caaatgaccg
    18661 gtgttgaagg ttatgtagaa tcggcagcaa gtggtcttgt agcgggaatc aatgctgtcc
    18721 gtcgcttcaa tggggagagt gaagtagttt tcccacaaac tactgctatt ggtgcactgc
    18781 cacattatat tacacataca gacagcaaac atttccaacc aatgaatgtt aactttggta
    18841 tcatcaaaga attagaaggt ccacgaattc gtgataagaa agagcgttat gaagctattg
    18901 ctacacgagc tttgaaagat ttagaaaaat ttctcaacta ttgatatatt catttgactt
    18961 tgccaacaag caaggtcaaa ttttctatat tgatttttaa aaatgtatga atggagtaaa
    19021 tatgcactat aaagaattac ttgagtcatc cgaaaaagct tatttggaaa ctaatcggta
    19081 taaacatttt aagaatataa tcacttcaga gcgttatttt tcaaactata ttagctacaa
    19141 aataatgcct acagtaagtg agtttttaca ggatatgatc tatttagcag atgaacaaaa
    19201 agattatcag tctgattttg cttttgtatt ttttgaggaa aatgctgaat tgagcagtga
    19261 attacagaga actttatcac aacaaggctt tgaatcagag aaacatatca tatttacaaa
    19321 ttcattaaat catcttaaac tttttgaaaa agaatttgat gacatcacat ttactaaact
    19381 taatgaaaat tacctagaaa aatacctaaa gttcaaatat aaacaaagta tagtttacgg
    19441 agaaatttac gctaagcaaa tgtataatga taatcgtgtt aatcttttaa ataataatgc
    19501 taaagtttac ctagctatta aagataatca aattattgga gatgtaacag cttgggacta
    19561 tggtgaatac gtggaaatag atgattttta tgtccaagag tcctacagag gacaaggaat
    19621 tggtacgcgc ctacaatttg atgcaagtag ggggtttaag aaagttatct taatttcaga
    19681 agaagaaaac agaaaaatgt atgagcacca aggttatgag gaagtcgcct attattggac
    19741 cgctttacgg agccctcgaa agtaaaatta taaaagaaat gatatagtta aaagctatat
    19801 ctcttgattg ataataagta ttttttaatc cattagcttt ttgttacact agtatcagtt
    19861 gattaatcat acaaccatat caaaggagaa ataaattatg aaaatcaaaa agctattagg
    19921 gttaacgaca acagtagtaa tttcagcact tattttagga gcctgtggtc agtctaaaaa
    19981 tgaagacgcc aaagtcgtta gagttggaac aatggtaaag agtaaaactg aaaaagctcg
    20041 ttgggataaa atcgaagaat tagttaagaa aaaaggcgtt aagctaaaat ttacggaatt
    20101 cacagattat acacaaccta ataaagcttt agaaagtgat gagattgata ttaatgcttt
    20161 ccagcattat aattacttaa acaattggaa taaagcaaat aagaccaatc ttgtttccgt
    20221 tgctgagaca tactttactt cctttagatt atactctggt actaagaacg gtaaaggtaa
    20281 ataccaaaca gtttctgaaa ttccaaataa agcaactatt actatcccaa acgatgcagt
    20341 taacgaaagt cgctctctct acttgttaca atcagcaggc ttgctaaaat tgaaagtatc
    20401 aggtgataca ttagcaacaa tgtcagatgt tgtttccaat cctaaatctt tagatttaaa
    20461 agaagtagat gcagcacaga ccgcacgttc tttggattcc acagatgcag cagttattaa
    20521 taatgatttt gtgacagaag caggaataaa tcctaaatca gctattttta ttgaaccaaa
    20581 aagtaaaaat gcaaaacaat ggtacaacct tttagttgct caaaaaggtt ggcaagacaa
    20641 atcaaaagct aaagccatta aagaagttgt gaaagcttac catacagatg cggttaaaaa
    20701 agttatcgaa aaaacatctc aaggtttaga tcaacctgtt tggtaaaaat aaataagcat
    20761 ggtcaattcg atcatgctta tttatttcga ttttttttga gtaattgact aggggtactc
    20821 aatacttctg ttaaccgttg ttgtcgtgct ttactttttt cggatttaga taaaagtagt
    20881 gtcttacttt cttcaaggat atcctctgta atatagtaca acttcactaa tgaaccgatt
    20941 ctgaccctac cagctttagg cgacatattc acaacttgat taggatgtag ggttgcccac
    21001 tttgaatttg gttcagcaac aatccttgat acattaaaat ggcgttgctc taagtaaact
    21061 tgcgcatcat tgatatcaag gtgtactaca ttatcaagct gagtataagt ctgttttttt
    21121 catgacgacg gtcaagttct ttttcaataa ttggacgaga attatcaatt gcttttccaa
    21181 taagttcagt tgtgtcaggg attattgaaa ttaatttacc aataggccct aaagattttg
    21241 tcaatttttt acttgtcttg gccatatgtt ttcaccgtac tttccatttg ttatgtaaga
    21301 tattatatca aaattatagt ttataaagct tttaaaactt tcaaatttgt aagaaatagg
    21361 tcttaagatg taaggttata gtaaaccatt ttgccatata atagacttaa agagaaaggg
    21421 ggagcaagag atgcggcgaa aaatagtttt actttttgta gttccaatgc ttatagtttt
    21481 aggaatttta ggagtggtag ttcattatta tggttcagca ttaaatattt atctattacc
    21541 tcctagtagc gaacgttatg gacgtgttat tttagatcgt gttgaacagc gtggtttgta
    21601 ttcgcaaggt cgtcaatggc aaattataag acaacgatca gaaaaaaagt tgaaaacgtc
    21661 aaaatcttat caagagagtc gcaacattgt acaggaagca gtacggtatg gaggcggtaa
    21721 acatagtcaa atattatcca aagagactgt acgaagagat accctagata gtcgatatcc
    21781 agaatatcga agattaaacg aagacattct cttaattact attccttcta taagtaagtt
    21841 agataaacga tctattagcc attatagtgg taaattacaa aatatattga tggaaaaaag
    21901 ttataaggga ctcattttag acttatcgaa taatactggc ggcaatatga tccctatgat
    21961 tggggggtta gcctctattt taccaaatga taccctattt cattatactg ataagtatgg
    22021 aaataagaaa acaatcacga tgaaaaatat accgctagaa gcattaaaaa tatctaggaa
    22081 aacaatcaat actaagcatg ttccaatcgc tattataact aatcataaaa ctgctagttc
    22141 ggcagaaatg acttttttat catttaaagg attacctaat gttaaaagct ttggtcaagc
    22201 tacagcagga tatacgactg ttaatgaaac tttcatgctt tacgacggtg ctagattagc
    22261 tttaactaca ggaatagtta gtgatcgaca aggatataaa tacgagaaca cccctatctt
    22321 accagatcaa gtaaccagcc ttcctttaca agagtcacag tcttggttaa agagtagaat
    22381 caatcaaaat tagggtataa taaataaagg agaattatat gttattagaa atcaatcact
    22441 tagaaaaagt ttttcgtaca cgtttttcaa aagaagagac aagggctcta caagacgtcg
    22501 attttaaagt tgaacaaggg gaattcattg ctattatggg agagtctggt tcgggaaaaa
    22561 caactctgct aaatatttta gctactttag aaaaaccgac aaatggacaa gtgattttaa
    22621 atggggaaga tattacgaaa attaaagagg caaaattagc gagttttcgt ttgaaaaatc
    22681 ttggtttcgt ctttcaggac ttcaaccttt tggatactct ctctgtaaga gataatatct
    22741 atcttccttt agttcttgat cgtaaacgtt acaaagaaat ggatcatcgt ttgtcagaat
    22801 tatcttctca tctgagaatt gatgacttat tagataagag acctttcgag ctctctggtg
    22861 ggcaaaagca acgtgtagcg attgctcgta gtttgataac caaccctcaa attttattag
    22921 cagatgaacc aacagcagcg ttagattacc gtaattcaga agacttgctg aatttatttg
    22981 aaactattaa cttggatgga caaactattt tgatggtaac ccattcagca aatgctgcta
    23041 gtcatgccaa acgcgtttta tttatcaaag atggacgtat ttttcaccaa ctttatcgtg
    23101 gtaataagaa taatagcgaa ttcaataaag atatttcttt aacaatgtct gccatattag
    23161 ggggtgaata atatgtttta tctcaaaata gcctggcata atttaaaaca ttctatagac
    23221 cagtacatac cattcctctt agccagttta ttactttatt cattgacttg ttctacgcta
    23281 ctaatcttaa tgagtgctgt tggaagagat atggggacag cggcaacggt tctttttctt
    23341 ggagtgattg ttttgtcaat ctttgcggta gtcatggaac attatagcta caatatcttg
    23401 atgaaacagc gtagtagtga atttggactg tataacattt tggggatgaa taaacgtcaa
    23461 gttgcgcgtg tagctagtct agagctgttt attatttata tatttcttat ttctatagga
    23521 agtctgttta gtgctttttt tgctaaattt atttatttaa tttttgtcaa cattattaac
    23581 tatcatgcac taaatcttag tttaagttta tggccattta ttatttgtat cgttatattt
    23641 acaggtattt ttctgacttt agaagttcca gttattcgac atgttcattt atcatcccca
    23701 ttaagtcttt ttagaaagaa acaacaggga gaaaaagaac caaaaggtaa tcttatactt
    23761 gcaattttag cgttagtagc tatcgccatc gcttatacaa tggctcttac ttcaggtaaa
    23821 gcacctgcat tagctgttat ctatcgtttc ttctttgcag tacttttagt tattgctggt
    23881 acttatcttt tttatattag ttttatgaca tggtacttaa aaaggttgcg tcaaaacaag
    23941 cattattatt ataaatctga gcattttgta tcaacttcgc aaatgatttt tcgaatgaag
    24001 caaaatgcag tagggttagc aagtatcact ttattagctg ttatggctct agttactatt
    24061 gctacaacag tctcactcta ttcaaataca caaaatgttg ttaccggact atttccaaaa
    24121 tcagtaagtt tatcaataga taattcaaaa ggtgacgcga aaaatatatt tgaagaaaag
    24181 attttgaaga aactaggtaa gtcatctaag gaagctatca cttataatca gacaatgatt
    24241 tcgatgccag ttagtcaatc aagtgaactt aatatcacat ctaaaaacgt caaacatgtt
    24301 gatatcacaa aaacgggttt tatgtaccta attactcaga atgatttccg tcgtttaggt
    24361 caccaattac caaaattaaa agataaccaa gtggcttact ttgttcaaaa aggtgatagc
    24421 cgtttgaaaa aaataaatct attgggaaat aaatttgacg ttgtaaaaaa tttaaaagag
    24481 gcttatgtcc cagagactac aaatacttac aacccgggtt tgattatatt tgcaaacaac
    24541 aaacaaattg acaatattag aaaagcatat ttaccttata ctaagaacat caatactttt
    24601 ccaaaaacct ttaaggcata tttggatttg aatagtcaag agataaattc aatttctaaa
    24661 aacgacataa tagaggtaga tggtaagtat gttggaaata ttagcactaa acagagtttt
    24721 ctaaaagaag gttatcaaat gtttggtggg ttattattta ctggatttct gttaggtata
    24781 agctttttgc taggtatagc tctaattgtt tactataagc aatactcaga aggtcatgaa
    24841 gataagagat cttatcgtat cttacaagaa gtaggtatga gcaaaaaatt agttaaacgg
    24901 actattaatt ctcaaattat gattttcttc ttccaacctt tagtggtagc agttattcac
    24961 tttggagttg ctattccaat gctaaaacag atgcttttgg tatttggggt gcttaactca
    25021 acaatcgtat atgtagtaag tggtttgaca gtacttgcga tttctataat ttactttatc
    25081 atttaccgta taactagtcg aacttattat catattattg aacgctaaaa aggattggtt
    25141 attttaccaa tccttttgca ttaatagaaa ccgatagact aaaaaatttg ttatactaaa
    25201 tgaaagaaag agatttcgta ctattttagg aggggatatg tcacaagagc aaggaaaaat
    25261 ttatattgta gaagatgata tgacgattgt gtcactttta aaagatcatt tatcagctag
    25321 ctatcatgtc tctagtgtca gcaattttcg tgatgtgaaa caagaaatta tcgcatttca
    25381 acccgatttg atactaatgg atattacgtt accctatttt aatggttttt actggactgc
    25441 agaattgcgt aagtttttaa caattcctat tattttcatt tcatctagta atgatgaaat
    25501 ggatatggtt atggcattaa atatgggggg tgatgacttt atttcaaaac cattctctct
    25561 agctgtatta gatgctaagc taactgctat tttaaggaga agtcaacaat ttatccaaca
    25621 ggaattaact tttgggggat ttacgttgac aagagaaggg ttattgtcta gccaagataa
    25681 agaggttatt ttatcgccaa cagaaaataa aatcctatct atcttgctca tgcatcctaa
    25741 acaagtagtc tcaaaagagt ctctattaga gaaactttgg gaaaatgata gttttattga
    25801 tcaaaataca cttaatgtta atatgacacg cttacgtaaa aaaattgtcc caataggttt
    25861 tgattacatt catacagtga gaggagttgg gtatttacta caatgatacg ccagttttta
    25921 agagaacact tgatttggta tattttatat atcatgatgt ttgtcctatt ttttattagt
    25981 ttctatctat atcatttacc aatgccctat ttgtttaatt ccttaggttt aaatgttatt
    26041 gttttactag gaattagtat ttggcaatac agtcgttaca ggaaaaaaat gttacatctc
    26101 aaatatttta atagtagtca ggacccctct ttcgaacttc aaccgagtga ttacgcttat
    26161 tttaatatta ttacacaatt agaagctaga gaagcgcaaa aagtttctga aacaattgaa
    26221 caaaccaatc atgttgcact tatgataaag atgtggtcgc accaaatgaa agttccattg
    26281 gcagctattt cattaatggc ccagacaaat catctcgatc ctaaggaagt tgaacaacaa
    26341 ttattgaaat tgcaacatta tcttgaaacg ttgttagcat ttttgaaatt tagacaatat
    26401 cgtgacgatt ttcgttttga agctgttagc cttagagaag tagtagtaga aattataaaa
    26461 tcgtataagg ttatttgtct atccaaaagc ttatctatca taattgaagg cgataatatc
    26521 tggaaaacag acaaaaagtg gttaactttt gctctttcac aggtgctaga taatgccata
    26581 aaatattcta atcctgagtc aaagataata ataagcatag gagaagagag tattagaata
    26641 caagactacg gtatcggcat actcgaagag gatatcccta gactttttga agatggcttt
    26701 acgggttaca acggtcatga gcaccaaaag gcaacaggca tggggttata tatgacaaaa
    26761 gaagtcttat ctagtctgaa tttgtccatt tcggtggata gcaaaattaa ttatgggact
    26821 gctgtttcta tacataaata aagttatgta aaataaaata tttacataac tttatttatg
    26881 ttactatagt ttatctaaag cgttcttttg ttcatcgtta acaatatggg tatataggtc
    26941 agtaacttga gtggaagcat gacctagttg gtggctaact aatacttgag attttgtagc
    27001 atcatatagc ctagtagcaa gagtatgcct tagtttatga ggtgttacac gaaccttaaa
    27061 atcctgggaa tacttagcaa ccattttctc tacacttgaa gaatcaatac gatttggcac
    27121 cccacggtat tctgataaaa atagagcaat gtcctgattt tctgctttgt atctattttt
    27181 acggatgtct aaataattag caaggtaggg cttcgcaaag ctagcgacat tgacagagtc
    27241 ccttttcccc ccttttcgtg tgacatcaat aaccattaca ttaaggttaa tatcctttaa
    27301 atctaagttt actgcctcag atagacgcac tcctgaagct agtagcaaag caataatagc
    27361 taaatctctc tctttatttt tgttgaaaaa agctaaagca cgtttggaaa gtttgttttg
    27421 atattcacaa tctatatact ctaagaattc tatcgtttca ttgcccaaaa ataacttttg
    27481 tttaatattt tctgcccgag aagctaaagt ttcctttttc tttttggttg agactttttt
    27541 catgacattg cgataaaagt aaggctcccc gtcagcattc tcaacctctt cagtaaggta
    27601 tttgaaaaga ctagataagg cagatagagt tctgttaatg gtggtttgag acaccccatt
    27661 ttgccttgtg ttagcattta ataaagggcg ttctcgtaaa tatagtataa aagcttccat
    27721 atctttttta gtaaggtttt ctaaaactga aagctcaatt tctgcaatgt gatgacaatt
    27781 agcaacacca gaatccaata accattcaag aaaacgtcta tattctttta aatattcgta
    27841 gagtgttgtg aaactataag gaacagaaag ctttgattga taatattcta gaacatacca
    27901 aggcataatt tcttttagtt catcgatttt ttcgagtagt aattcacgtt tcataataaa
    27961 atctccatta tatctataaa tagaagtata tcatactcta tcataacttt caagaaaatt
    28021 atagtttttc ggaaagttta aaagttaaaa acaggaattc attcggtaag tacatcaaat
    28081 atccattctt tatgatgatc agtattactt attatcgttt aacatgtttg ttatagtaaa
    28141 gacagagtat tttgagaaaa ctgattcata atttcattga ttttatcggt atctgaaaag
    28201 gtaataaagt gtactcaatt atttttaatt atatttgcaa gttaataacc aaagttaaat
    28261 tctactttaa aatatactag ttttctcaat attagatcta ctatgtctta taaaaaaagg
    28321 tttaggagga ctggtattat gtctagtttt aatagaaaaa aacttaagtt tttgggtata
    28381 tctttggcga ctctaacagc caccacagtt accctggttg cctgtggtaa tgagtcaaag
    28441 aactctggag ataataaagt tattaattgg tatattccta ctgaaatcag tacattggac
    28501 atttcaaaga atacagatgc ttattcaaac ttagctatag gtaattcagg cagcaattta
    28561 cttagaattg ataaagaggg caaaccaaaa cccgatttag ctaaaaaagt atccgtttct
    28621 tctgatggtc ttacttatac tgctacactt cgtgataatt taaaatggtc agatggaagc
    28681 aaactaagcg ctgaagattt tgtttacaca tggcggagaa tagttgatcc taaaactgca
    28741 tctgaatatg cttacttggc gacagaatcg caccttttaa atgctgataa gattaattct
    28801 ggagatatta aagatttaaa taaacttggt gtaacagcta aaggcaatca agtgaccttt
    28861 aaattgacaa gtccatgccc tcaatttaaa tattatttag ccttctctaa ttttatgcct
    28921 caaaaacaat cgtatgttga aaaagttggt aaagattatg gtacaacatc aaaaaatcaa
    28981 atttactcag gtccttacct tgttaaggat tggaatggtt caaatggtaa atttaaatta
    29041 gtcaaaaata aatattattg ggattctaaa catgttaaaa caaatagtgt catagttcaa
    29101 actatcaaaa aaccagatac tgcagttcaa atgtacaagc aaggtcagat tgattttgct
    29161 gaaatatcag gaacttctgc tatttataac caaactggtt cggttaaagc attgacaaat
    29221 cagaaaatta ggcaagcttt gaatttagcg actgatcgta aaggtgttgt taaagcggca
    29281 gttgatacag gttcaactcc tgctgaatct ctggtcccta aaaaattagc caaattacct
    29341 aatggtgagg atctttccaa gtatactgcc ccaggttata cgtataatac aagtaaagct
    29401 caaaaacttt ttaaagaagg tcttgctgag gttggtcagt catctctcaa gttaacaatc
    29461 acagctgatt cggactctcc agcagctaaa aatgctgttg actatgtgaa atctacatgg
    29521 gagagtgctt tgcctggtct aactgttgaa gaaaaatttg ttactttcaa gcaacgcctt
    29581 gaagatgcaa aaaatgaaaa ttttgatgtc gtgctcttct catggggagg agattatcca
    29641 gaaggatcaa ctttctatgg gttattcacg actaattctg cgtataatta tggtaaattt
    29701 tcttctaaag agtatgacaa tgcctatcaa aaggcaatta ctactgacgc cttgaaacca
    29761 ggagatgccg ctaatgatta caaaactgct gaaaaagctt tatttgatca atcatattat
    29821 aacccagttt attacctagg gaaaaaagga ctccaaaatt ctaaattaaa ggggttggta
    29881 agaaattcaa ctggtttgaa tgttgatttt acttatgctt ataagacaga ataaaagaaa
    29941 agcgttagga tatttctagc gcttttctat accaaaagta cccaatgagt tatcaaaaca
    30001 cttataattg agaacaatga tccaattaaa taatattcgg caaaaacctg acttttagaa
    30061 attttatcgt aacgtgcaat ggattttgca gtaaaaacaa gacctatagc agcatactgt
    30121 cctgagatta gtaatatagc cataattaga cgctctaaat taccaatcat agctcctgct
    30181 ccttcaattg tttcttcatg atcttcagat ttttctttca ttatttttgt tttttcttgt
    30241 gttaataatg atgataattc tttagcttga aatttattaa agaaaatttt aaacacaata
    30301 ttaatgggtt tggtgataat gataaaaacc agagctatga caataaagtg cttgataggc
    30361 aaaagccaat gattaggcgg taaatatgta ggaagcgcat ggtaaaaaac gataatgcaa
    30421 gtatagtggg ctagttggtc tataataaaa gctgcttttt ggaaatgccc ttcctctacc
    30481 caagggtaac tcaatttctt tatactatca ataacaatat gacttcccca tatgcttaaa
    30541 tttaacaaag tagtttctgg tatcaatatc attaaagctg ctagggggag gaaaacaatc
    30601 aagagatgtc tccagagtcc cctccaacta tgtgatttac tatctgctaa ggattgactt
    30661 tgccactgaa agtcagctaa aaaatgtgcg caaaataata aagttaggat tggattatcc
    30721 cttaaaaaat cagaaatcat agcttccccc tttagtttga gtgcaacttt taactaatag
    30781 atcggctgcc tgtgttctcg ttcttaagta aatcttcaga ccgcttgctt taaggcgttt
    30841 agtcagcgca ctaggttcaa tattttccag ttgggctaac ttttgatgtt gaaattgttc
    30901 ttgataatta tcttgaagta ttaagtgctc aagcatttga aaatggtttg tagtccattt
    30961 tgacttgata aaatcaccag ctgaaatgag actatttagt gttaattcaa ggttttggtc
    31021 ttcatcatca aggcaaatag ctacttgaac tgttccataa tcatttttat catgtatatg
    31081 attaatagct gagcgagcat gccagtaggc aggaccatca gcaccgatac tttcatttga
    31141 attgatggat gttataatgt ttcctgtacc gaggccgaac cttacattaa caggttttag
    31201 agctagttga atatggtcaa taatttgaaa tacctttttt gattgtttca ataaagcttg
    31261 aaattcatca ccagctgtaa tagtgaatgg agaaatcagc tcttcaccat atacatcaga
    31321 tagttcggtc attagttgct gaaaagactg ttggaaagtt tcacgttcaa gtatctgttt
    31381 tgaattaatg atatcaccga ttaatgctaa atacataaat atcaccacct ttttgtctat
    31441 tatagcagac aaaaataaaa atgtctattt aaatagacat ttttatttgc gtttcttctt
    31501 agctttcttc attttattag ccatacgttt cattgattgt ttagctgcaa aggctcctac
    31561 ttttcctttt aagccaccac caaacatttg gctgaagtcc atatcgccac ctaaactaga
    31621 taagtcaggc atgccatttt gccccatcat accttctaaa ttagacatat ccattccgtc
    31681 cattccaggc atatctttag ggaggttatt aggatcaatt cccatttttt tcatcatttt
    31741 attcatatca ccggacatca cgccttgcat catttgtttt gcctggttaa aatctttaat
    31801 gaatttatta acatcaacga aagtattacc tgatccagca gcaatacgac gtctacgact
    31861 tggattgagg agatttggat tttctctctc ttctggagtc attgatgaca caatggctct
    31921 tttgcgagca atttcgtttt catccacttt gaagtttttc attgctggat tgttagccat
    31981 tcctggaagc attttaagga gatcttccat tggccccata ttttgaactt gatctaattg
    32041 atcaataaaa tcgttgaagt caaaggtgtt ttcacgcatt ttctccgcta attccattga
    32101 acgtttttcg tcgtattctt gactagcgcg ctcaataagt gttagcaggt ctcccatccc
    32161 caatatacgg cttgccatac gatcgggatg gaaagtttca atatcagtga ttttttcccc
    32221 tgtccctgta aatttaattg gttttccagt aatttcgcgt actgaaaggg cagcaccacc
    32281 gcgtgtatca ccgtcaatct ttgtcagaac aacaccacta atgctgagct gacggttaaa
    32341 ttcttcagcg acatttgcag cttcctgtcc aatcatgctg tcaacgacaa ggagtatttc
    32401 attaggttga gctatggctt tgacatcatg caattcctgc ataagggttg catcaatttg
    32461 taggcgtccg gctgtatcaa taagaacata gtcattacga ttttcacgag cctgttctaa
    32521 acctttagtg acaatctcaa ctgctgaatg gttagttccc atatcaaaga caggaacatt
    32581 aatctggcta ccaagggttt ttaactgatc aatagcagct ggtcgataaa tatcagctgc
    32641 tatcatcata ggacgagcat tgtcttcttt gattagtttg tttgctaact taccggcaaa
    32701 agttgtttta ccagcacctt gtagaccaac catcataatg atggttggaa ttttagggga
    32761 tttttcaatt tcagaggtct cagcccctaa taaatctgtt agctcttcat tgacaatctt
    32821 aacaatttgt tgcgtaggat ctagtgtatc aatgatttca tgtccaacag cacgttcacg
    32881 aacatgttta ataaaggttt taacaactgg aagagcaacg tcagcttcta aaagagctag
    32941 acgaatctcc ttggtgactt cttgcacatc tttttcagat aactttttct taccacgaat
    33001 atttttaaaa actccttgca ggcgctctgt taaactttcg aaagccataa attatttctc
    33061 ctcatttaat ctctattatc aatgctactt agaatggaaa ttttttcttg taaaaaggca
    33121 tcatcggtat atttttctag aatgtcatca aaaatctgac tacggacaat ataatctgaa
    33181 tacatatgta gtttcatttc gtacgcctca agaatttttt cagttcgttt gatattatca
    33241 tagacagctt gtcggctaac accggattct tcagctattt cagcaaggct gtagtcatcg
    33301 gcgtagtata actcgatata attcatttgt ttatctgtta ataatgccgc ataaaactca
    33361 aaaagggcat tcatacgatt tgttttttca atttccatag cttttattat accaaatttc
    33421 ctatcagctt gctatatcaa gtatgcaaga ccactttaaa atttaaaagg ttaggactaa
    33481 catggcctaa ccttttactt ttggtagttt tatcttaaaa atactgcctc taggtttatt
    33541 atcaattact gtaatattac ctcgcagtga gttaacgatt tgttgagcta aagaaaggcc
    33601 gaggccaaac cctccttgtt gtcgtgttct tgctttgtca acacggtaaa aacgatcaaa
    33661 aattctgact ttatcctcct ctgaaattcc tggtccatta tcagcgattt ctaaaaagag
    33721 atacttatca gtttcagata tggtaaagtc aatgctacca tcattgtcag tatattttat
    33781 agcattatca aataaaatag tcatgagttg ttttagcagt gtcttatctg ttttgaaact
    33841 atcttggatc atattatgtc cagtaaaatt tttaccattc tcttgggcga ttagatcata
    33901 attctcaaag atactatcaa aaagtgtagg cttaataaca gctaattctg gtttgattcc
    33961 atcatcacgt cttgctaagt ttaaaagatt agtggttaaa atacgcatat ttcgtacttc
    34021 atctagactt gaagcgatat tttcactatt ttctaaaatg gtggcattcg gttttctgaa
    34081 tagcgtttct aggcgatttt gtaagacagc taggggagta cgcagctcgt ggctagcatt
    34141 ttcgacaaaa gccttctgtc tttcgtagtt ttctataatc ggtttttgtg cccatttagc
    34201 taagtagata cttgctccaa ttgaaataat ccaaaaagta gtcatgacaa atataataat
    34261 tttttcatag cgttctttag catttactaa ttgatcaata ttaacaatag ctgtcatgta
    34321 agtcacatta ggataagcag ggttattaac ttttacggtg acagcatgat atgtctcatc
    34381 ttgaccaaag atgttggtta aagttaattc actaatgcca cctagatttt ttttattagg
    34441 ttttaaaaac tgatagtttg aaaatgcgtc aaaatagtta atgacttctt cttctgatgt
    34501 aaaaagaata atatcagtat tagctactgt ctgaccgccg ggttttaact tgacacttgc
    34561 gatattggag ttatctaggt aggcagcagt acgtgacata accatattga tgtaattttt
    34621 aggatgggta ctgatatact ttaaactact atcaacagaa gaataaacac catagcgcat
    34681 gacttggagg atgataacag tcataaccag aaaaatacca ctaaaaactg tgaaaaaatg
    34741 gataaaatga ctatctgaga catttttttt gaatttactt atcttaatcg gtttcgacat
    34801 gctttaaaat atatcctaca ctacgcaaag tttgaaggtt ttctgaaaaa agtgttcctt
    34861 ttaatttttt ccgaacctta gagacataga cttctacaac tgaaatcgtt gtatcactat
    34921 caaatcccca aatacgatca aagatttgag atttcggtaa gataacattt tgattttgaa
    34981 ggaaatagac taaaagatca aactctttac ctaagagctc aacttctgtt tgattaacaa
    35041 aggttgagtt agtagacatg tctacgcgaa tatcaccata aattaggctg ttatcattaa
    35101 attttccaga acgctttaaa agagcctgaa ttcgcatttt tagttcttct aggtaaaaag
    35161 gttttgttag ataatcatca gcacctaagt cgaaaccttg tcccttatca tcaatgcttt
    35221 ctttagcggt cattattaat acaggggttg taataccttt ttcacgtagc tctttcaaca
    35281 cttggaagcc gtttttttca ggtaacatga gatctagaag aatcagatca tagactccac
    35341 tttcggcttc gtaaagaccc tcttctccat caaaaatttg catgacatcg gcaaagtcat
    35401 caaggaaatc aaaaacggaa ttggaaagac tcaagtcatc ttcaattaat aaaattttta
    35461 tcatagtata tcctttcgat gtacatattt tatctgaact attaatatta gaataccaaa
    35521 ctttgcttaa taaatcctaa ataaagtatg ttttatgtca actaatttca tgacacagac
    35581 aaagaaatag agattgaata atccatcaat ctctatttga gtttattaga tatatttttt
    35641 gatagtattg ataacttctt ctttttgctt cgtgattaaa aggacacgag cagaaatttc
    35701 tttaattccc attgaaatat tacggctaat cgccatatcg cttaattgag gctcgaaaaa
    35761 gtttttatat tgttcaaggc gttcttctgt tttgaaacta cttgagggat aaatcacaaa
    35821 tttatcaaaa ctcatatcac cacctagagt tgctttgatc cattcccagt tttcttgggc
    35881 ccactcccag acactttctt gtgtaaaaga atttttaagg aaagtagcat accaactcat
    35941 agctaagtct tgtggtttca caatatcttt atttttccaa tctgacaata atttcttgag
    36001 tgtcttgcta tctgtagtat gagataaagc actcgctagt tgtcgtttga aattcccatc
    36061 ggtagtagta ctataagtct ctaataacaa gtggctaagt tctttggatt cgaaatgctt
    36121 catttcattg gtaagaacta acaggcgaat tgcagctggt atagcagcga tattattttc
    36181 gtgggcttta aaaatatggt gagcttcttc aatagcatgt tcattatctg agtagagagc
    36241 aaggcttagg gatagatgac ggaccatttc gctatcatct ccttctcctt ctcttttttc
    36301 aaagccaagt ctattaaaat taaatgctga taatttatta acaaattctt taaagtcgaa
    36361 ttcatcttgg ctatcttcct ggacgaataa ggataaacca tcaataacac tcttaatagc
    36421 agagttaaca agatatgatg tctcttggct caaaagagag actaaaggta ttaaactact
    36481 atatgaaatc ataccacttt cagcaagtaa atgacgttct tgtacaattt gtagtttaga
    36541 aatgttatcc ataagaggaa gatcagaaat gatatgctct aagagttgac cttgatagtt
    36601 agtaatgtaa tgtgcagtat tttcagtatt gaaacgcagt gcaccatttt ctttgttctt
    36661 ttcagctaac tgactaaaat tggggattac taccgtttcc tcggttaata tctctgggat
    36721 tccttcccaa ttactattaa gaggaatttg ccacaagcga gacttatctt cgtgttcacc
    36781 aataaagaat tgtttttgag ttaaaatgag ctcatcttct tctattttgg ctgccatcac
    36841 agggtatcct ggttgctcta accaagaatc catgaaagct gcaacatctt ttcctgaggt
    36901 ttgagaaagt gcattccaaa ggtcacgacc aatagtattt tgatattgat gtttttcaaa
    36961 ataaattttt agtcctgcgg caaaatctgt atcgcctaac cagcgacgta acatgtgcat
    37021 gaggcggcta ccttttgcat aaacgatagc tggatcaaaa agtgtgttaa tttcatcagg
    37081 atgattgacc tcaacgtgaa cagattggac gccatctgtg gcatcacgct taagagctaa
    37141 tggtagacca cctgtttgaa agtcttcaaa tatattaagt tttggttcaa tataatcaat
    37201 tgatacatat tccatcatat tagcaaatga ttcatttaac cataaatcat cccaccattt
    37261 catagtaacg aggttaccaa accactggtg agctatttca tgtgctacaa ctaatgctac
    37321 ttgctgacgg ctggaaacac tagagttctc atctactaaa agatagactt cacgatatgt
    37381 tactaatccc caattttcca tagctccagc agaaaaatca ggtagagcaa cgtggagtga
    37441 ctgaggaata ggataacgta caccaaagta gtcctcataa aactcaataa cacgcacaac
    37501 gatatctaaa gaaaaatcta attcatttaa ctgatgggct tttgtggcat atgagccaac
    37561 aagagtgccg tttttggtat gtgttgtttt accatgcaat tcccccaagg caaaagctaa
    37621 taaatatgag gacattttag gtgtggtgtc aaatgtccat aaacctgttt cttgacgttg
    37681 ttcagcattg atttccggca tatttgaaag agcaatctct ccttcttttt gatcgaattt
    37741 taggctcaaa tcaaaggttg cttttgcttc tggttcgtca atacttggga atacttcacg
    37801 agcaaaatga ctttcaaatt gtgtggagat aacctctttt ttgatgccat taactgtata
    37861 gtaagatgga tacatacctg tcatattatc ggtaatgtga ccgctgaatt caaagactaa
    37921 taccatgcta ccagtttcat gcaattgaat atgcatagct tcattgtctt catctagttg
    37981 gaaatctaat ggttgattat ctaataagac agatttaatg gttaaccctt tttggtgaaa
    38041 agagatattg ttatctaagg cttcgccaga cacagcaacg ttacctgaaa aggttttagt
    38101 ttgacgatta atatccaaga aaaggttata gttttcaggg acaaatttgg taacaaaatg
    38161 ttctacagtt ttcatataac tcctaactaa ttcagaaaca tttgaaacat tactagtcct
    38221 gtatgggcta gaaaagatag aaaagctgta acaagatagt tacagctttt ctatcgcacg
    38281 aactatactt acaattacca ctctacaagc aataattata tcataactcg ataatttttc
    38341 cagtttttaa ataaacaatc cattcacaga tatttttgcc ataatctcca attcgctcca
    38401 agtgcatcaa tacttggaag tattctttgg cggcaaatgc tacgtcagga tgcttttgaa
    38461 ttgtttcaac taccattttt tggatatcac ggaagtaatc atcaactatt tcatccattg
    38521 ctgctacttc gtaagctttt tcatcatcac cattgatata agctgttaag gcttcttcaa
    38581 gcatgttttt aactgctttt cccatattat tgatttctaa ttctactgct gggattcttt
    38641 cctcgccctt catacggatg gttgcttttg caactgctgc tgcatggtct cccatacgct
    38701 caacatcact agtagctttt aaaacggtaa taacggtgcg taagtcttgt gatacaggct
    38761 gctggagtgc aataatttcc aatgattttt tctctaactt cgtttcaaag ttgttaagcg
    38821 ttacatcatc ttctatcact tctttagcta actctcgatc atgactgaca aaagcacgaa
    38881 ctgttttttt gatttgtccg atagcttcaa ttcccattgc atagaattgg ttatgcagtt
    38941 tatctaattc ttcatcaaat tttgatctta acatattgta ttctctttct gatcttatta
    39001 tatggtatta accgaattta cctgtaatgt aatcttcagt ttctttatgt ttaggattca
    39061 taaacatttc ttttgtattg ccatattcaa ttaagttacc gtctaagaag aaacctgtac
    39121 gatcagaaat acgtgaggct tgctgcatag aacgtgtaac aagtaacatg gtatattgat
    39181 cttttagacc atgtaaagtc tcttcaattt taccagcaga aattgggtca agtgcagagg
    39241 ttggctcatc tagaaggata attttaggct tggtagcaag ggttctggca atacaaacac
    39301 gttgttgttg accaccagat aaaccaagag cagagtcgtg taatctatct ttgacctcgt
    39361 cccagataga cgcgcccttc aatgaagtct ctacggcttc atctaacgta gctttgtctt
    39421 ttattccttt gagtctaaga ccataaacaa cattttcaaa tactgacata ggaaatggat
    39481 taggctgctg aaaaaccatc ccaatttctt tacgaagttc tacggtatca gtgcgcgggc
    39541 tataaacatt atgtccgtta tacatcactg ctcctgttag agtgacttca ggatttaagt
    39601 ctcccatacg gttaatagct cgtaagagtg ttgactttcc agaaccagac ggaccaatta
    39661 aggctgtaat ttcattagga tagaagtcca ttgagacttc ttttaaagct tttttcttat
    39721 tatagtaaac agacaaatca ctgacttgta aaataggttg tgtcatcttt tacctcacta
    39781 tccaaatcga ccagaaacat aatcgctagt agattgacat ttagcatttt ggaaaatgtt
    39841 attcgtttta tcgtattcga ttaagtctcc aagatagaaa aatgcagtat aatcactagc
    39901 acgtgctgct tgttgcatat tatgtgttac gataattata gtgtaatttt tcttgagttc
    39961 aaacattgtc tcttctagtt gcatagttgc aatcggatcg agtgctgaag caggttcatc
    40021 cattaagaga atttctggtt taactgcgat agcacgtgca atacataatc gctgctgttg
    40081 accgccagat aaagtgaatg ctgatttatg aaggtcatct tttacttgat cccatagagc
    40141 agcttgtttt agtgaggttt cgacaacttc atctaagaat tttttatctt ttacaccagc
    40201 acgctcatat gcaaaggtaa tgtttttata aattgatttt gcaaacgggt ttggacgttg
    40261 gaaaaccatt ccgatatgtt ttcgcatctc gtaaacattg atatcttgag cgttaacatc
    40321 aattccttca tacatgattt gtccagtcac gcgtgcaata tcaatcgtat cgttcatacg
    40381 atttaagcta cgaagatatg ttgattttcc acatccagat gggccgatta aggctgtaat
    40441 tttatttttt tcaaattgca tatcaattcc cttgatagct tctttctcac cgtagtaaac
    40501 gtgtaaatct tttgtggtga gagctgaatt ttcttcaggg aaggtaatga tatggcgttc
    40561 gtcccaatta tattctgcca ttactactcc ttttaattag acgaggttaa tttcgaatga
    40621 agtttcttac cgatcgagcg agctgaaagg ttgaaaataa gaataacaac caataagact
    40681 gcggcgctac cggcagatac ttgtgttgca tcagggattg tcccttcgct attaactttc
    40741 caaatatgaa cagctaaagt ttctgcttga cggaaaattg agattggact tgtaacacta
    40801 aggacgttcc aatttgacca atcaagtgct ggagcagatt gaccagctgt ataaataagt
    40861 gctgctgcct caccgaaaat acgtcctgaa gctagcacaa caccagttac aatacttggc
    40921 agagcttcag gaacaacgac gtaaatgacc gtttcccaac gagaaatacc tagggccaat
    40981 cctgcttcac gctgtgtgtg gtgtacattt tgaaggctat cttcaacact acgtgtcatt
    41041 tggggtaagt taaaaacagt aagtgctaag gcacctgaga taattgagaa gccatattga
    41101 aattgaacaa cgaagattag gtaaccaaac agaccaacta caacagaagg aagagatgac
    41161 aaaatttcga tacatgttct aacaaagttt gttaaacgtc cttttttagc atattctgag
    41221 aggtatattc cagctcctaa agatagagga atagaaataa tcagagtaac aataagtagg
    41281 aaaaaggaat tgtataattg tattccaata ccgcctcctg cttcatatga tgatgatttc
    41341 ccagttaaaa atgaccaaga tacgtgtggc aatccgcgaa caagaataaa aattaaaaga
    41401 gaggcgagga ttgtaacgat gatagctgca atactataga gtattgtagt tgctaatttg
    41461 tcagcctttt tagcgttcat agtttttctt tccttccctt gtaattaact taataactgt
    41521 attaaatgct aaactcataa ttaaaagtac taaggctagc gaccagagga cattgttttg
    41581 tacagttccc attacagtat taccaatacc cattgttaga acagacgtta atgttgctgc
    41641 tggtgttgtc aatgatgttg gtaaaattgc agagtttccg acaaccatct gaattgctag
    41701 agcttcacca aatgcacgtg ccataccaaa aacaattgca gtgaaaattc ctggacgtgc
    41761 agcgtttaat ataacacgcc aaattgtttg ccaacgagtt gcccccatgg ctaaacttgc
    41821 ttctttatag tgacgtggca cagctctaag gctatctact gtcatgaaag taacagttgg
    41881 tagaatcatg acgaagagga cacagacccc agataaaatg ccaaaacctg ttcccccaaa
    41941 aatagagcga acgaagggaa caataatctg taaaccaata aaaccgtaaa caacagaagg
    42001 aatcccaact agtaattcaa ctgcaggttg taaaatttta gaaccatatt taggtgagat
    42061 ttcagtcata aatacggcag caccgatagc aaatggtgta gcaatgatag ctgatagaat
    42121 agtaacaata aatgatccag ctatcattgg aagagcaccg aatgacttag cacttggttc
    42181 ccattttgta ttaaataaga agtctgttaa tttaacgcca tcaacaaaaa atgtagatag
    42241 tcctttttga gcaacaaaaa tcaaaatcat cgcaacaatg aagacgatta gggcaagcga
    42301 aagaaaagtt attcctttac caaatttttc taatcgagag ttttttgaag gagatgttaa
    42361 ttttttagct aattcttgat ttttcataat tcctcctagg atcaagatgc tttatctaga
    42421 tgtgattaag ttttattttg gggttacttt accttcagca tctttaacaa ctttcatgtc
    42481 gttaatagag atatatccca tatgttgtac gatactactt tgaactttat cacttttcat
    42541 gtagtctaag aattcttttg ttagacctgt ttcattacct tttgtataca tatgttcata
    42601 tgaccaaagt ttccagttgt cggtagtaac atttttagct gttggcgcaa aaccatttag
    42661 ttttaatgtt ccgacagatt tatcaacgta tgcaaaagcg aggtatgaaa tcgcaccagg
    42721 tgtttgagaa acaattgatt tcaccatacc atttgaatct tgttcttggc tttgaatagg
    42781 agcaacattt cccataatag tattatcgaa ggttgcacgg gatccagaac ttgctgctcg
    42841 attgatgatg gaaatatcta aatcttgtcc tcccacttct ttccaattct tgatttttcc
    42901 tgcaaaaata tcacgaagtt ggtgggtggt tagattttta acattaactt ttttattaac
    42961 aataacagct aatccagcta ctgcgacttg atggtctttc aatttcttag catcaatacc
    43021 atctttttct tctgcaaata agtcgctatt accaatttga acagctccag actgtacttg
    43081 tgataagcct gttcctgagc ctcctccttg gacattgata gtcttaccga ggtttgtctt
    43141 accaaactca tctgctgcag cttctacaag tggctgtaag gcggttgaac caacgctggt
    43201 gattgattga cctttatcaa tccaattagc acaaccagac agcgtcaata agacggtggc
    43261 tagcaaggtt aacaaaaaaa tgtttttttt cattttcatt ttcctattct tttgaataaa
    43321 ttttgatatt tagtagttga tatatattac taaaatcatt gagtgtttaa tgatttacaa
    43381 atagtaaaaa cacacatcac tgtcccatag taccataagt ttctagggtt tgaaaagtat
    43441 ttctttttcc ttttttatta aaagtattca ataacagtta tgtctgaaat cgtagtcctt
    43501 tagggtaata atttttaaga gtattatttg tcacttttgc aaaacctaat ccattgcctt
    43561 ttacaagaat ttgataccaa ccgtttggaa cagtcttgtc tagtttgact acatttccag
    43621 atacgtacac ctcaaactgt tctatatcaa tttcaataga ctgtactagg tcatgttcgc
    43681 taagtgccat tcctaaagca aacgatggct caaaacgatt cttcttgaaa gtgcctagat
    43741 ggagaccatt gcgtgctatt tttaatttag acaaatcagg gagaccatta ggtagaagat
    43801 agagttggtc gccgaaaaca tctaaaacac cgttcaagtc tatctttaaa tggtcttgtg
    43861 caaattgttg ccacaattgt aactgcattt tatttatttt ctgtgctttt ggcttaattt
    43921 tctgtgcttc cttagatcta gtatctctca gttttgcaac gaattgtcct tcaccttgaa
    43981 aatgatgagg gtacatcctt gcaacttgtg gtacattaat cccttcaacc atcccattta
    44041 gtttaggaat atcaacaagt tctaagtaat catactcttg aaggagccaa ttaaccactt
    44101 cttcgttctc ttctggtgac catgtacaag tagagtatac aagtatacca ccatgagcta
    44161 gcattttgat tgcttctttt aaaatatctc tttgtaactg tgcacattca gtggggtaat
    44221 ctttgtgcca atattgtatt gcttgaggat ctttgcggaa cattccctca ccagaacacg
    44281 gaccgtcaaa aacaataaga tcaaaaaaag aattaaaaca tttggctagt cgttgggagc
    44341 tttcattagt cacgataaca tttctagcac caaaacgttc gacattttcg actaaaattt
    44401 tactacgttt atttgaaatt tcattactaa ctaatagacc agtattgttt aaatatgata
    44461 gaaggtgtgt tgtttttcca ccaggtgcag ctgctaaatc tagcactttc attccttctt
    44521 gtggttcagc aatctgagcc actatctgag cagcaggttc ttgagaataa acaaggccag
    44581 ttgtgtgttc tattgatttt cctgatattt tcccataatg cccccaaggg gtacttggga
    44641 tggcatttgg aaaatcaagt tgcttttctt tgagaggatt ggtgcgatat gccgatatgg
    44701 gtttttgttc aaaactgtca aaaaaagctt ctgcttcatc tttaagtata gtctgatatt
    44761 tttcaataaa ttcattcggt aatttcatgt ttctattata ctacataatc acgggcgtgc
    44821 aaaaaaaacc ttctatttaa ttagaaggtt ttaagtttat tttttggtaa ggtaggattg
    44881 gataagattt aatttcgact ttggtaggaa cattactttt tgtcttgttg aatagttggg
    44941 ttcttccccg tcaagcgtta ataaggtaaa tcctaatgtc tcccccatga tttttgctgc
    45001 agcataatcc caaggttgaa tatacgaaaa ataagctaat aatttgccct gcatgacttt
    45061 tgccatgcta attccagcac caccatagat acgaacacct aaggtttcag aagctaaatg
    45121 ggcaataccg caatcatttt ctgcatacat agcagaattt actccaagta agcaacgttc
    45181 taaaggacac tcttgaaaag gaacaatttt tttatcgtta gcatagacat caaaatgtcc
    45241 tcccccgctg taaagaatat cagccataac gtcgtagatg ataccaaatt gtccaacgcc
    45301 ttcttcgtaa taagctaaca taacagcaaa gttatctttt tgaacaataa aattaacagt
    45361 tccatcaata gggtctaaaa cccaaacatt accttgtgca atgggtgagc gcacatcatc
    45421 ttcttcggca agtatattat catctgggta atgttgaata atttcttgaa ttaataattt
    45481 ttgtgttttt ttatcaagac ttgtcactaa atcatcaaat cgagactttt cctcaacatc
    45541 aaaagtattt tgcatctcag atttaatgaa ttgaccagct ttatacacta attgtttagc
    45601 gaaatcaaat ttagcgtcca agtgaaataa atcctttcgc ttgttgttgt gctgctttaa
    45661 cagcttgata agtggaataa ccgctagttc tctggaattc acggtcaatt tgtttttcct
    45721 gcgccttgga tttaacaaat tttttaaatt ctttatagct atctagcaaa tcatcaactt
    45781 taacactatt ttcataggcg tgttcaactt gatttaaaaa gcgaagtacc ttagtaatat
    45841 cttccgtatt ccatgaagga tcaagaggat aactataatt actactaatc atgttttccc
    45901 tcaatttcag cacgaatagt ggcctgtttc agttcttgtt tacgatagtc acgcggtaag
    45961 aaggcacgaa tttcatcctc attaaaacca atctgcatac gttttttgtc taaaataatt
    46021 gggcgtcgta aaagacttgg attttctgaa attaattcca ttaatgaaga cgttgataat
    46081 tcgtctacat caatagcaag tttttgaaag acttttgaac gtgtggaaat aatgtcttct
    46141 gtaccatttt ctgtaaacga tagaatttgt aatagttctt ctttattgag tggacttgta
    46201 ataatgttat gctcttcaaa agcaacctca tgcttactta gccaagcacg cgcttttcga
    46261 caactagtac agctgggtga taaaaataag gtaatcatat aaccgcctct tgtatttact
    46321 agagactatt atacaaaaaa ttaaagagtt tgactatctt ttttccaatt taaagcaatt
    46381 tctttatctt ttttaagttg ttttacaaga tcgtcaatac cattaaactt taccatttct
    46441 ctgattcttt ttaaccagaa aatttcaata gtttctccat aaatgtcgcc atcaaaatca
    46501 aagatattag cttctaaacg aagttctgta ccaccaaaag tgatattttt tccaatgctc
    46561 gtcattgctc tataatattt tccattaatc agaacattgg aaatataaac accgtctgca
    46621 ggtaagtaag tgcgattaat aggagctaga ttagcagttg ggaaccctat agttcgtcct
    46681 ctagcatctc catgtactac catgccacag gttgaaaact cataacccaa aagttggtta
    46741 gctttctcaa cattaccctc tgtaatcaat tgacgaatac gtgtagaaga gattttttca
    46801 ccaccttcag aaatttcatc aataatatag actggtccct tgaaattacg cgctagataa
    46861 tcaccactgg tacgattatg accgaactta taatcaaaac cgacaacagc agcttttgct
    46921 ctgagaccat aaatatattt cttaataaaa aggtcagaag atacttttga aaaatgactg
    46981 gtaaaattca ttaaatataa ctcatcgacg ccatactctt gaaatttttc agaacgtttt
    47041 tctggagagg taagatgtag caataattca ggttggaagc gagcgaaggt tagacgaggt
    47101 gtctcattaa acgttaagac gacaattttc aaattttttt cggtcgcgat ttttttagct
    47161 ttatcaaaga gggctttatg tcccagatgt aagccatcaa aatagcccaa aactaagacg
    47221 caatcttctt tagcttgaat atctttataa tcattaatgt aatggatttt catgattgac
    47281 tttctttata acaatacttt gtgtggttta aattcattgc cacgtttttc tagtatagcg
    47341 ataactctat tttcataaaa agctgctaag gtatctgctt cattttctaa taagatacga
    47401 cgtccgtatg agatttcaac tttatcttcc tcagttaggt taacttttgg gagatctaaa
    47461 acaccatatt caattggaag taaaaatgaa aaatcttctt gcttatattt ttcattaatt
    47521 tcttcaagcg ttaatgattg tgtaatagat aatcctgcag atgaggttct ttttaggaaa
    47581 gacatatggc tagcatatcc tagttttatt cctagatcaa cagctaacgt cctaacatag
    47641 gtacctttac tgcatgagac atagaaacta aaacggcaga tgcctttttc gaagtacaat
    47701 gggcttgtgc gtctaaattc agaaattgtt atttgtcgtt taggtcgctc tacttcttct
    47761 cctgaacgag catattcata gagtttttta ccatttactt taactgctga atacatagga
    47821 ggaacttgtg taattggacc ggtaaaactt ttcatcgcat gatccacaac atcctcgcta
    47881 agatcggatt gagttagtgg agttcttgaa atgacctctc cactagaatc ttctgtactt
    47941 gtggcatatc ctaaagtgat ttctccttca taaattttgc cactttctgt catatattca
    48001 ataacacgag tggcttttcc aaccgcaatt ggtaaaaccc caacaacatc tggatcaaga
    48061 gttccgccgt gtcctatttt ttttgtatgt aatatctttc gaagcttgaa tacagcatcg
    48121 tgtgatgtca ttccagcttc tttctttaaa ttaattattc ctgtaatcat gttttatatt
    48181 atatcagtaa ataagcggta acacaaagaa aggagcgtaa aaacttacgc tcttttggta
    48241 tagtgctata aggatttata cctaatctaa atagtatgaa aagcctgtac taaccttctt
    48301 gaaccctagg ttttcataaa aatgataagc ttcctgacga tattctttta aactgctatt
    48361 taaagccagt acttcacttc catctgtttt agctagctgt ttaacgtgat taataagttg
    48421 actagcgatg cctttacgac ggtgcttttc attaactact agtgctaaaa tacgatagta
    48481 agtcccactt ttttcaaaaa agtaaagttt atgatatcca acgaaggcga gtagtttgcc
    48541 attatcctca gctatcaaaa agccataatc tgaatgagta agaataacct ttaaaataga
    48601 ttgaatctct gataatgata ttgcgtatcc taattcttga tacaaaggga ctaatttttc
    48661 actatcgtct agtgtagcag ttctaatctt cataatcata acctcttagt cttttagagc
    48721 atcgaatttt gctttcattg ttggattttt tcttgttaat ttcttgacag aaacatcgtc
    48781 taatttatta tttgcaagac gtagttgatt ttcagacgtt gttaaagcag ccttaacagc
    48841 ttccatacgt ttaatagatt tatctatttc atcgattgct ttctggaaat ttttgcttgc
    48901 agaattataa tttttagcaa atgcattttt gaaaatatct aaatcttctt caaaatgtgt
    48961 gatgtcaata ttttgttctt tcatcaaagc aagctcttgt ttatatttta aggtattgag
    49021 tgctgcattt cttagaatac caattaattg gataaaaaat tgtggacgta taacgtacat
    49081 tttagggtat ttgtgactaa catcaacaat tccagtatta taatagtcat tgtctgcttc
    49141 aagcatagtt actaaaactg cgtattcgca agatttttca cgacgatctt tatctaattc
    49201 tttgaaaaaa tcttcatttt tatgcttctt gatagtatca tcagactcat ttttcatttc
    49261 aaacatgata cttaaaaact caaggtcatt ttcatccttt tctcgataga taaagtcccc
    49321 ctttgagcca cgacttgata atgtattgtc cttctcaaaa taagcattag gaaaggccaa
    49381 atgtcgcact ttattaaatt ctgtttcagc ataatgttct aaactttctc ctactgcttt
    49441 agtagactgt tgagctttga agtttttata gaattctact tgttcattgg ctgcctttag
    49501 ttgtacttca taatcacttt ttactgaagc taaagctaaa tctttttctt tttcttgaat
    49561 gtgcaactga ttttgtattg catctctttc tcgttcgact tttgttagcg cctcttgcaa
    49621 gctattttca tgctctaaac gtagtctatc gatttggttt tcaaggtcgc gaagcatcat
    49681 gtctttttga tttaattgct cagcgagttg gtactcattg tctttgagaa gattgtcttt
    49741 ttctataaga aattgtttga ctttgtactg aagtttagct atctccttgt ctttttctac
    49801 aacaacttcc tgtaattgat tttttgcttg ctcccctaat attagacgtt ctttttccaa
    49861 ccgttcactt acttctttgt caaaagcatc tccacgaatt tgttctagta attgatggta
    49921 ttcagactcg ttgatggcaa aagctgttcc acaatgaggg catttgattt cgttcatgat
    49981 aactcttcct atgatgtttt gtatttggtt ctagctaagt tgttgtctga tgtcattttg
    50041 gatacgttcc cactcctgct ctgcacgtgc tatcttacgt ttgtaatcgt tgagcccctg
    50101 tttataacgt tggataagat aatcctgtct aaaaagcggg atacttggta ttgcaatatt
    50161 taacagttcc tttgtgctaa tattcatcac agcttttcct ttgttagcat tctcaagtgc
    50221 ctgttgacct tcttctgaat caaaaaaaag ctttaagtag taccctctaa tgtgttgtgt
    50281 aggccttagt attgtaatat tagcagaggc aacgacaggg taatcttgct cttcaaaaat
    50341 agcaactttc ttgacagttc cttttgaagc gattaaaaca tcgccttcct gaagtagata
    50401 ttttaaaagc gatcgttgtt catcttggaa tgttcttaag ttatggtagt caattcctaa
    50461 aggagacata tcactcaaat taataatacg gatatcacct gcttctgctt ttgaaggcac
    50521 agctttacct ttaaagcagt ctaccaattc agataataaa taagtttttt tctctgggtt
    50581 acttgttgga ttttgtggca caatagactc ctttcgtctt taaaatatta tatcacaaaa
    50641 catagttttt taatactaaa ataaaaaata agctttcagt aagagaagct tatttagatt
    50701 aaatctatta gtcgtaagga aaattcgatt tttgataaag ccaataacat gctacgataa
    50761 gaacaatact ggtaatcatg cttccttcca caccaaattt tccaccgcta atccagtcag
    50821 caccataggt tttaacatgg actagggagt tagacaacat tggctctcca ctaacttcga
    50881 ttccaaagac actcccttgt acacagttcc aggcgccatg aataccacac gccccccaga
    50941 tgttaccttt taaaataaca tagagcgata gaaatagtcc aaagagacac agattgaaaa
    51001 tcgagataag gcttacatga ttattagcgc tatgcagaag accgaaaaag attgaactga
    51061 tactgacaga aactatctta ctatactttg tggcactcac cggaagtaac catccacgac
    51121 acaatatttc aacagatgcg ctttgtacct gccatccaac agcaaaaata agaaaccaaa
    51181 tcaatttatc agctgtaaaa cgaacagttt cgataactcc agagcctgaa attattgtta
    51241 atattaagca ggctccaaag agaacgctcc cgactaacca tcctctagat aattctgtta
    51301 gggctccttc tttaaatagc cctagagaac taattggacg cttctcttta aatttcaccc
    51361 acataaataa cattaccgta ctaattgaag ttgctagtaa aatgattaac tggacgatat
    51421 cgctatccca ctctagtttc caaaaatcaa gcgagataat caattcaata ataatatagg
    51481 taaggaaatc tgctgtaaat agtaatatca aagccaataa tatagttaaa aggacatgcc
    51541 ttgatacttt accttttgca gtttcctcaa aaatagactt tctcataaat atctcacttt
    51601 tatctaactt tatactgtta tattatacaa ttaagattac ttgctgtcaa tcgttatcga
    51661 aaagattaga aaagataatc ttttttataa actaatctac tgatttcaag gcatctaaca
    51721 tattgagttt ttttaattga tggttaacta taaaacctag acttattaga atgatgacta
    51781 ctacgcttat tggtattata aatgtagttg gtgatacttt ttcaccaaaa agtatttgat
    51841 ctgaaccaat tagcaacatc atttgacggt gtaaataagt accgctaatg gtgcctagaa
    51901 tcacaccgat ggttgataaa ataatggttt cgcggtaaat gtataaagtt acttcttcat
    51961 tataaaaacc taataccttt atagttgata gctcccgctt acgttctgct aaattaatat
    52021 tagtgagatt atagagaatt acaatggcta acaataaaga tacgacaact aaaatagtca
    52081 tagagccatc taaagaacgc acaacggctt ctacactagc cagctgaaga gcattttgcg
    52141 taagagactc aactccatta atttctaaaa gtttttctgc taagttattt gcgattttat
    52201 gtttggttaa cttaaccaaa aaagcgttat ctttagcttc tttcttaaaa atagttcgat
    52261 agtattgttt tgtcataaaa atatagtgac caacattcgc attgacaact cctgttattt
    52321 ttaattttct agaaggaagt gtttgtgaat gagataaatg gataaaatca ccggtattaa
    52381 catgataaaa ttgagctaat tttttagaaa taataatccc tttattattc aagtgcaatg
    52441 acttattagt tgttgcttct tgtaaataaa taaaaggtag taattgtttt ttgtctgtta
    52501 tgaataggga aattgactgt ttattaattt gtccaggaat ggattcgtca agattttcaa
    52561 ggtgtattgg ttggtatttg tctatttttg aatcttttga aagtagttca agtatttttg
    52621 ggggagaagc atttgtgtta tatgttagca atatatcata gggcataata cgaccgaaat
    52681 gttcattgac ggtttgcttt aaggaggatt gtattcctag acctgaaaat aagagcgcta
    52741 ctgatccagc aactccaaat atggtcataa tcatcctttg tttgtaacga aaaatatttc
    52801 tagcagttac tttttgtgtg aaggacagtt ttttccaaat ccaattgata tactctaaag
    52861 ctattttaga tcctttagct ggtggttttg gtaataataa aaatgctgct ttttctttca
    52921 actcacgtcg agtgataaca aaaactggaa gaacaatgct taataatgaa aaaacaaagg
    52981 ctagtaaact gtaatttaag aaaagatgat actgtgtgtt tggaatagtc aggtctttgg
    53041 aaatgatatc agaaataata cgaggtaaaa gataatgccc tccaatgata cccaaagtag
    53101 tccctaatgt tcctgctata aaaccataaa taataaattt aaagataact tggcggtcac
    53161 tatagcctag tgcttttaat atgcctgcgt tggttctttc ttcctcgacg aacctagtca
    53221 ttgttgttaa ggttactaat gctgcgacta aatataaaat aatggggaaa agattactga
    53281 ttttagaagt gctatttgta ctggagtcaa aagtgttata tccttgtcct ccaggaaagg
    53341 tagtacggtt gtagacaata atttgaggcg attccattat ttgggtgtta tgtctcaatt
    53401 ttagaagctc ttttgttcgt ttcaaaagag atagtcggtc ttgtttaatt ttcatttgaa
    53461 tagcttgtct ttgagatagt ggcatcgcta ggctatcatt atctaaccga ttgatttgtt
    53521 tttgagcttt tagtaggctt ttttcctcct catcgatttt acgtaggctc tctttttttg
    53581 ttttggtata tcttatttta ttgtgaatat ttaattttcg cgataccttt tcttggtatt
    53641 tcgctacttt ttctttgtaa cttattgaga aagggttaat caacctaaga tctttaaaag
    53701 ttacccttgc cattgtatat actggagagt gaaatgctgt cttatctaat acgccataac
    53761 tatctaagtc gccgtcccct gtttgagaac taccaaggtt tgtcttagac caaatctcgg
    53821 tagaattaac aaaacctaca atagttagag gacctgtatt tgaaaacagt ttgtttttat
    53881 cttgtttaaa attaatttta tcacctattg catatttttt tcgttcctga aatgagagag
    53941 caatttctga tttatttaaa gggagacgtc ccttaacaag atcaaaggtt gatatcgttt
    54001 tagtattgga atacaatcga taagatttac tattctgttg aggggttgta tctacaaagt
    54061 aagaaaattc aacttgtgat ccttttaagc tttctaaaac agtttgatca tgtttgtcaa
    54121 gtccccaaga attgaaaacg gtaatatcca taacatgatg atgagcaagg taattttgtg
    54181 ctgttctttg catattaggt gctgagacct tcagtcctat aaaagcaaat gagcctaaca
    54241 tcataagtaa gaggattgat gaaaatctac cttttgaagt cgtaattgat cgatagatat
    54301 ctttccaaaa tgtctttccc atctgtataa cctctaatat tctatgttct gaatatcact
    54361 tggattctcg tttattacaa tatcactgat ttttgaatca tgcatatgga tcacgcgatt
    54421 tgcaattggc gctaaggcag tattatgagt aacaatgatc acagttgttt cttttgattg
    54481 cgccatcttt tgcaggatgg ctagtacttg ttttccagtt tggtaatcta aagcaccagt
    54541 aggttcatca caaagtaaca gtttaggttt tttggcaata gctcgtgcta ttgcaacgcg
    54601 ttgctgttcg ccaccggaaa gctgtgctgg aaaatgattg atacggtgtc ccaaacctac
    54661 gttttctaat gcctgttggg catctaatgc ctttggaaca atttctgaag ccaattctac
    54721 attctctagt gctgttagat tagggactaa gttatagaat tgaaaaacaa acccgacatc
    54781 atatctacgg taacgagtca attccctgat ggtataatta gcaatattct taccatcaat
    54841 taaaacttca ccttcactat tactatccat ccctcctagt atattgagaa cagtagattt
    54901 gccagcgccg gatgcaccaa gaataaccac caactcacct ctttcaatac tgaaactaat
    54961 atcattgtta gcaacaattt cggtttcacc tgtttgataa cgcttatagg aatgcttcat
    55021 ttcaatatag gacataacgg cacctcacac tatgaataca cctatattat agcacttttt
    55081 tattaattct cccataaaaa taaatacata aattaagtta tgtaaaataa taattttttt
    55141 aaaaatctga gctaaacact cagattccac tttatttaat tttctcttct tggtagtcgc
    55201 atttttcatt tgagcaaaca acttgtttac caccaccgcg tacttttttc tccactaaga
    55261 aatcatttga tttggggcaa gttcgtccaa taggtttgtc ccaagatgta aactcacatt
    55321 ctgggtagcg gtcacagcca taaaagatac gattgcgttt tgtttttcgc tcaataactt
    55381 gtcctttttg acagatagga caaataacac ctatttcctt agtgatagct tttgtgttat
    55441 gacattcagg gaaattgcta cacgcataaa actttccata acgtcctagt tttattacca
    55501 ttggtgatcc acatagctcg caatcaaatc cagcaggttc atcctttatt tgaattttct
    55561 ccatttcagt ttctgctttt gcaagttctt tttcaaatgg tttataaaat tcatcgataa
    55621 tcttttgcca ctgctcttta ccaatctcaa cttcgtctag tttcccttcc atttcggcag
    55681 tgaaggtaac atcaacaata tctgggaaaa attctacaat aaggctatta acaatttcac
    55741 caagttcagt tggttcgaag cgtttagctg caagcttaac ataataacgt ttttgaatag
    55801 tctcaagcgt tggggcataa gttgaaggac gacctacacc gttttcttca agtgttttaa
    55861 tgagacttgc ttctgaaaac cttgcaggag gttgagtgaa gtgttgttca ggattcgtat
    55921 taaccttttt aacactttct ccttcctcca tatctggtaa cattttattt ttgtcagtat
    55981 cgttataaac agccatgtaa ccatcaaact tgacttggct accattagca ataaaagtaa
    56041 caccattttg cgttaaatta actttcattg tgtcaaagac tgctgctgtc atttggcttg
    56101 caataaaacg gttccagata agagtgtaaa gttttagttg atctttgtct aaatacttag
    56161 caatactttc gggtgtatga ttaacactag atgggcgaat agcttcgtga gcgtcttgtg
    56221 ctccagaagc atttttaact ttattcccat gtttagaata atttgcacca aaacggttag
    56281 taataaattc tgttgcttca ttttgtgcta acggactaat acgtgtagaa tcagtacgca
    56341 tataggtaat cagaccttga tgacctgctg tgcctaagct aagtccttcg tacagctgtt
    56401 gcgcaatcat catggtttta cgagttcgaa aattgatttt attagctgca tcttgttgca
    56461 atgaagaagt tgtatacggt aatggtgcat tacgacgacg ctcttttttt tcaacttttt
    56521 caactaagaa ttcatcagtt ttaatacgtt ttagaactgt tttgacgtct tcattattcg
    56581 ataatttgaa tttctttccg tctaaaccgt agaaagtggc attgaattta cgtgttcctt
    56641 ttttaaagga accgtcaata gtccaatact cctccggttg gaaagcctta atctcatttt
    56701 cacgatcaat gattagttta agagcaactg attgtacacg tccagctgat agcccttttt
    56761 ttactttttt ccataaaata ggtgagatag aataaccaac aatgcgatct aaaacacgtc
    56821 gtgcttgctg agcatcaacg agatccatgt taatctgacg aggttccaca aaagcatttt
    56881 taacagcatc ttttgtgatt tcattgaaaa caacacggtt tctgtcttct ttgtctaaat
    56941 ctaaaatatg cgctaaatgc caggaaatag cttctccttc acggtccggg tcactcgcga
    57001 ggtaaacttt tttagccttt ttagcttctt tttttaaatc attaatgaga ggtcctttac
    57061 cacgaatatt aatatattgt ggttcgtaat tattctcaaa atcaatggac atacttgact
    57121 tttttaaatc acggatatga cctactgagg ctacaacttt atagttacgt cctaagtatt
    57181 tttcgatagt ctttgctttt gcaggagact ccacaataac tagatttttt ttagcagtag
    57241 ctgatttttt tttacttgtt tttttggtcg acgtttttgt cgtagttgcc ataggtatca
    57301 ctttctatat cgtaaactta tagcataata actcactttt cttaaactgt caatagaaaa
    57361 cttctcctat agtagaaagc aaaacactac tgatattcag aaaggatatc ttttcctgaa
    57421 ataatgcatt tagctccttc ttggataaga tggtggcatc catctgattt gccatcgata
    57481 atatttccgg gaatggcaaa aacttctcgt ccctcttcta atgctctttc acaggtaatt
    57541 aaacttccag atctcatctt agcttcggca accacaatac cttgacacag ccctgcaata
    57601 atacggttac gttcggggaa atgaaatttc aagggttgtt ctccggtaaa atattctgat
    57661 aatacgagat gattatatga catatattct tgaagtttct tattctccgt tggataataa
    57721 acatctaaac cactcccgat aacagctata ctactgccgc cattttttaa agcactaaca
    57781 tgtgctgctg tatcaatgcc acgcgctaaa ccacttacga taacaaattg attgttagtc
    57841 tccttgataa tctttttaac agactgacaa cctatctgag atgcctgtct tgcccccact
    57901 acagctaatt taggtttaga tagaagttca atattaccct gataaaaaag tagaactggt
    57961 ggattatata tttcttttaa ctctaaagga tagttagaat ccaaaatcga tagaactgga
    58021 aattttttga attcttgtcg aagtttttta aggtctagct gtttataatt ttctataaag
    58081 aaagtaggat ttttacattt tgatactacg gccatattgc gaacagatag agaagttaaa
    58141 ctattctttt tgagatagtt gataatattg ttgatattaa gatttgttag tccagctttt
    58201 ttaagcttaa atagttcaaa atgattcata aggttacctc cattgactat attcgaaaat
    58261 aatagacaaa ataaagtgat aagatcaatc tatactctga agcattgata atagctattt
    58321 tttcaaaaaa ataagactag gatttttcct agcctttgac aaaaaattaa cgattatcaa
    58381 caaagttctg tacatcttta atcatacgga gtgttactcg gcttccgcct gaattgatat
    58441 accaatcttt tccatctacc tcatgaacac gtttgttttt gacagcatca gttgctttaa
    58501 taacatcgtt attaaaaagt tcttttgatg aagctccttg tccaatagtc gctccacgat
    58561 ctaaaacaaa gagatagtta ggattttttt cagcgatgta ttcataagat acgggagtac
    58621 catgtgaact tagtttttca ttttcattga ctgctttaaa tccacctaca gagaaaatcc
    58681 aaccaaaacg accagaaggt gattgagtta aaagttcacc gctgtttgcc attacaaata
    58741 gagctgtagg tttacctttt ttctcaatag ttttctgcaa tttaagaaca gcttgtgcga
    58801 tatctttatt aaaggttttt gcctttttat tttggtcgaa gattttccct aacattgtga
    58861 cacgctcagc aactcctttg tcaaatactt tttttgagtc gactccagca tatactaatg
    58921 ctgctttagg tgcagcctcc tttaattttt caatattatc aacagaagcc atacgtgctc
    58981 ctaggaaaac cacatcaggt tgcatacgag caatagcttc aaagtttggt tcgaagagac
    59041 ttcctacgtc ttggtatctc ttagctttat aaacagattt gacgttattg ggcaataaac
    59101 ttagagcatt tttagccttc gggataccaa taacagattt ttcagctcct aaagctgtga
    59161 tagtatccaa agctcccata tcaaaaacaa ctactttttt gggttttaaa ggaactttaa
    59221 gtgtctcgct gatttgcttt ttattatccg gcttgtctct tttttctttt ttaatataga
    59281 ccgaatagtt gttttttatg gtaacagttt gtgatttttc tttagctaaa acagcttgag
    59341 aagtggttaa gatagtgcat agtgctaata tagcaataat aagttttttt gtcattaaat
    59401 aaaatctcct aaaatagata aaaactaaaa atgttatcct acataaatct ctttatttac
    59461 gttgtacaaa aaaggataat tggtttacca tcaacagttt cgatacggat aggcatatca
    59521 aataaatgag aaaggatttt ttcttgcata acatcttcga cagtcccaca agacaccact
    59581 tgtccttctt ttaaagcgac aacattgtca gcgtagtgag ttgcaaaatt gatgtcgtga
    59641 ataataatca ctatagtttt attaaactca tacgcatatc gtttgataag atccatcatt
    59701 tgaacagcat atttcatatc taaattgttg agtggctcat ctaagcaaat atattgagta
    59761 tcttgtgcca ttgtcattgc gataaaagct ctttgaattt gacctccgct gaggttatta
    59821 atgaactcgt cagctatatc gtttaaatcc atataagata taactgattc tatgacagct
    59881 ttatcttttt gacttaaccg tcctttacta taaggaaatc taccgaatcc tatcaagtca
    59941 ctcaccttta tcttgagtgt taagtgatta gattgtctta atatggctaa tgttttggct
    60001 aagtcatttg aactgtagtg ggctaatggt actttatcaa ggaaaatttc accattatca
    60061 taaggttcaa gtcgactgat acaactcagc aatgtactct ttccagatcc atttggacct
    60121 aaaatagctg ttacttttcc tttaggaatc gaaatattaa tgtcttttaa tatttccttt
    60181 tggccataga acttatgtaa attgtttatt tgaatcataa gtttttcctt cctttgatta
    60241 aggtgaatat aaaataactt cccccgataa ggttgaggag aacacttagt tgaacagtga
    60301 ggtgtaatag attttgaacg agatgctgcc ctaagacaag tgtaaagata cagataacaa
    60361 tggctatagg aactaatatt tgatgtctaa aggtatgaaa actgtgatag gtaatatgtg
    60421 ctacaagtaa gcctaaaaaa gtaatcggcc caactaatgc tgttgctata gctaccatca
    60481 atgctaacca taaaaaaatt ctagtagtaa gcttttgata tgagatgccg agtgatatcg
    60541 cattttcttt acccaatagt aagacatcca attcctttat aaaaggccaa ttaatgacaa
    60601 agctaacgac aatgataatg aaagaaatcc aaaggatctt ggtattaata gcattgaaac
    60661 tagcaaagag ctgattttgt aaaatcataa aatcgttggg gtccataatg gcttgaataa
    60721 aggatgagat agaagaaaat aatgtattaa atatgagtcc agctaataga acaaaataca
    60781 aagactgttt tttatttcga aaaagtatcg taaataataa gtaagcgaat ccaaccatta
    60841 ataataatga aaggaggaag ctgctaaaag atgaaaattt tatgacacgc tgagctccga
    60901 ttaaatacat taatcctgtt tggataagaa cgtataagct atctaaacca ataatggacg
    60961 gagttaaaag tcggttattg gttagagttt gaaaaataag actagaacta ccaatgcaga
    61021 taccaaccaa aattaatgca atcaattttt gataacgtgt ttttaggata tagataatca
    61081 aaaattcatt tgcgtcagtt ggaataccat aaattaaaaa caaaataatg gtagcaatta
    61141 gtagtatcag taaaataagt aattttggta tctctaaatc cttaatcatg attttccctc
    61201 atttcttata attgaaatca agaaaaaggc actacccaat actcctagcg taactcctat
    61261 tgagacttca taaggaaaaa taactaagcg accaaaaaga tcacatatca tgacgataca
    61321 agcccctaaa agcgatgtct ctaagatagt tgaggacata tgatcccctt tagttaagct
    61381 aattagatta ggtacgatta aacctaagaa aggtaaagct cctacgataa taatgactag
    61441 ggatgtcata attgaaacca aaactaaacc aaagtaaatc atcttttcat aagcaatccc
    61501 taaattttta gcgaagcttt ctccaagccc aacaatggta aactgatgag caaaaaaata
    61561 cactaaaaac aaacatggca atgctaagta aagaatttcg tagcgatggc tagtcatgat
    61621 ggaaaaatta ccttgaagcc aactgttgac agattgtagt acttgaaaat gtgttcctaa
    61681 aaatactgtt gctgctgcta caatttgtcc aagcatcagt ccgataagag gtataaaaat
    61741 attatcctta aaagtaatca tttttaaaat agttacaaaa agcagcgatc ctaaaatggc
    61801 aaaacctgaa gcaatacata gttgaataaa aatagaggta tttttaaaga aaatcagcgt
    61861 aacaactact cctaatttcg cccaatccat agtccctgaa gttgtcggtg aaacgaactt
    61921 gttttgggtg agttgttgca ttagcaggcc acagactgac aaacttgcac cagatattag
    61981 gatactaatg gttcttggta agcgactcgc taaaaaaata tctacctgtg attgattaag
    62041 gtgtgttatt tgctccagtg ggatagattt aacaccaaca aatagggacg ttagagaaag
    62101 tataaccaaa gtacaaccca ttacaggagt ggttaatagc tttttcataa aactccttaa
    62161 aatgaaagat aaagtgttca aaaatgagca caaatctata ataacataat tttttggaaa
    62221 aatatctact ttttcaaaga attt
//
LOCUS       DATRUK010000002        61270 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.002, whole genome shotgun sequence.
ACCESSION   DATRUK010000002 DATRUK010000000
VERSION     DATRUK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 61270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61270
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.002"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(258..941)
                     /locus_tag="VBE34_000058"
     CDS             complement(258..941)
                     /locus_tag="VBE34_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262819.1"
                     /translation="MDKLKKLQKENTLLEGRIDNSNNQTYTDMIVYLRGASISPYHQE
                     LIRNDIVNMLLEAQERQASLVSVFGEDRHDFINQVIKSTPKISKKEETLQRWDLAILL
                     LTIQMIIFLGGYLITEALQQSVPDLIPITLLDVLFAIFISIIAVKITDTIIYATYNFD
                     KSKEKKYFFRYMFLILSLIIAYILIGKYCHLPFINIPLWIYLIILGLSFSLHIIVKKY
                     LNKHYYIEF"
     gene            complement(962..1273)
                     /locus_tag="VBE34_000059"
     CDS             complement(962..1273)
                     /locus_tag="VBE34_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEO7262820.1"
                     /translation="MIPSQMLKGLLEGAILKIIQQEETYAYEISKKLNSYGFGEISEG
                     TIYPIILRLQSNKYIKGQQKPSPQGPKRKYYTLTNEGVEQLAQFKQNWESLSKALNKL
                     L"
     gene            1464..2171
                     /locus_tag="VBE34_000060"
     CDS             1464..2171
                     /locus_tag="VBE34_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEO7262821.1"
                     /translation="MKIDKKELLALIASIILLIFASVTFFLFKDHGTTQMDTVESSVN
                     HVSDSQLTEAQDMLDKFEKKPSEKLLKDVELALNKLSNSSKKEALQKRFKKAKDKYLK
                     DEADKKATKDATDLVEILEQAPSEENVLKAEAAVKKLTVKESKEALQKRIDTVKTQYG
                     LIGNQTPSSSVAETTEQGTANPASQDTSSYVNQDVAPTYEQPQTNNTPVTPGVNNTVP
                     TPGTGTVPATNGTGVAQ"
     gene            2449..3597
                     /gene="nagA"
                     /locus_tag="VBE34_000061"
     CDS             2449..3597
                     /gene="nagA"
                     /locus_tag="VBE34_000061"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEO7262822.1"
                     /translation="MTKYIKADRFFYADHVKENGYLEIKDNHFGKWIENISGQEEILD
                     YSGYQIAPGLVDTHIHGFAGADVMDCDSEGILRMSAGLLSTGVTSFLPTTLTSDTKRL
                     EEASKAVAAVAGKEQGAKIQGIYFEGPYFTEEYKGAQNPIYMRNPNLEEFAQWQKAAK
                     GLITKIALAPEREGVEEFVSAITKQGVTVALGHSNGTYKEAKKAVKAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVDPVACDILMTQKGHNHVALITDCMAA
                     GGAPDGDYMLGELPVVVSNGTARLKDGGNLAGSILKLKDGLKNVVEWGIATPEEAVRM
                     ASYVPAVSVGIDNVCGQIKEGYAADFIVLDKHLDLVATYLDGEKRWQS"
     gene            3715..4257
                     /locus_tag="VBE34_000062"
     CDS             3715..4257
                     /locus_tag="VBE34_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEO7262823.1"
                     /translation="MSIIIGLMAAMFIIRLAYLKLSIANEKALRKNGAKEYGVGVSKA
                     ITVLHIIIYFSSVTEAVLTKASFNFVSVIGLSLMIFSVFMLHTVTRLFGRIWTVKLMV
                     DKNHQFVDHWLFRVVKHPNYFLNIAPELLGVTLLCHAKYTALFVLPIYAFVIYLRIRE
                     ENLLLKTIIIPNGIKKSRVS"
     gene            4570..5484
                     /gene="glyQ"
                     /locus_tag="VBE34_000063"
     CDS             4570..5484
                     /gene="glyQ"
                     /locus_tag="VBE34_000063"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEO7262824.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLKSLEL
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLQTGPVTSE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFTQPEYEHSKYSFEISDQVMLLENFE
                     KFEREAKRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEETRIKLLAEED"
     gene            5484..6125
                     /locus_tag="VBE34_000064"
     CDS             5484..6125
                     /locus_tag="VBE34_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7262825.1"
                     /translation="MNTLIVNSHPDFSNPYSFTTILQEKFIELYNEHFPNHQLSILNL
                     YDCVLPEITKEVLLSIWSKQRKGLELTADEIVQAKISKDLLEQFKSHHRIVFVSPMHN
                     YNVTARAKTYIDNIFIAGETFKYTENGSVGLMTDDYRLLMLESAGSIYSKGQYSPYEF
                     PVHYLKAIFKDFMAFDDFCVVRAEGTDILDRQVVLDKMNQDLREAFEAFYSKE"
     gene            6129..8168
                     /locus_tag="VBE34_000065"
     CDS             6129..8168
                     /locus_tag="VBE34_000065"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEO7262826.1"
                     /translation="MTKDLLLELGLEELPAYVVTPSEKQLGQKMVKFLEDHRLSFETV
                     QTFSTPRRLAVRVKGLADQQTDLTEDFKGPSKKIALDAEGNFSKAAQGFVRGKGLSVD
                     DIEFREVKGEEYVYVTKHETGKSAIDVLASVTEVLTELTFPVNMHWANNSFEYIRPVH
                     TLVVLLDDQALELDFLDIHSGRISRGHRFLGSDTEISSASSYEDDLRQQFVIADAKER
                     QQMIVNQIHAIEEKKNISVEIDEDLLNEVLNLVEYPTAFLGSFDEKYLDVPEEVLVTS
                     MKNHQRYFVVRDRDGKLLPNFISVRNGNAEHIENVIKGNEKVLVARLEDGEFFWQEDQ
                     KLNIADLVEKLKQVTFHEKIGSLYEHMDRVKVISQHLADKAGLSDEEKLAVLRAASIY
                     KFDLLTGMVDEFDELQGIMGEKYALLAGEQPAVAAAIREHYMPTSADGELPETRIGAI
                     LALADKFDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILEAFGWDIPLDELVTNLYG
                     LSFASLDYANQKEVMAFISARIEKMIGSKVPKDIREAVLESDTYIVSLILEASQALVQ
                     KSKDAQYKVSVESLSRAFNLAEKVTHSVSVDSSLFENNQEKALYQAILSLELTEDMHD
                     NLDKLFALSPIINDFFDNTMVMTDDEKMKQNRLALLNSLVAKARTVAAFNLLNTK"
     gene            8180..8437
                     /locus_tag="VBE34_000066"
     CDS             8180..8437
                     /locus_tag="VBE34_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEO7262827.1"
                     /translation="MDPKKIARINELSKKKKTVGLTGEEKVEQAKLREEYIEGFRRSV
                     RHHVEGIKLVDDEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            8526..8789
                     /locus_tag="VBE34_000067"
     CDS             8526..8789
                     /locus_tag="VBE34_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEO7262828.1"
                     /translation="MIELLLALLLWNILVFATYAIDKRRAIRGAWRISEKTLLIESLL
                     LGGFGAFLGGEVFRHKTTKWYFRLVWYIGMIIDLAIIWLIWCY"
     gene            8904..10412
                     /gene="glpK"
                     /locus_tag="VBE34_000068"
     CDS             8904..10412
                     /gene="glpK"
                     /locus_tag="VBE34_000068"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEO7262829.1"
                     /translation="MSSEEKYVMAIDQGTTSSRAIIFNKKGEKIASSQKEFPQIFPQA
                     GWVEHNANQIWNSVQSVIAGAFIESSIKPGQIEAIGITNQRETTVVWDKKTGLPIYNA
                     IVWQSRQTAPIADQLKQEGHTNMIHEKTGLVIDAYFSATKVRWILDHVPGAQERAEKG
                     ELLFGTIDTWLVWKLTDGLVHVTDYSNAARTMLYNIKELKWDDEILELLNIPKAMLPE
                     VKSNSEVYGKTTPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMN
                     TGEEMQLSQNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLRMIETSSESEGL
                     AQSSTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRD
                     VIDTMQVDSGIDIQQLRVDGGAAMNNLLMQFQADILGIDIARAKNLETTALGAAFLAG
                     LSVGYWESMDELKELNATGQLFQATMNESRKEKLYKGWRKAVKATQVFAQED"
     gene            10425..12254
                     /gene="glpO"
                     /locus_tag="VBE34_000069"
     CDS             10425..12254
                     /gene="glpO"
                     /locus_tag="VBE34_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEO7262830.1"
                     /translation="MEFSRETRRLALQRMQDRTLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVTNTPAANKVLSAEDVLKREPDLQKEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGAYIASHVKAEDFLFDDNNQIIGVRARDLLT
                     DQVIDIKARLVINTTGPWSDTVRNFSNEGKQIHQLRPTKGVHLVVDRQKLNVSQPVYV
                     DTGLNDGRMIFVLPREDKTYFGTTDTDYHGDLEHPTVTKEDVDYLLNIVNKRFPEAEL
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKLSDESFEELIDSVKDYIAHKNHREDVE
                     KAISHVESSTSEKELDPSAVSRGSSFERDDNGLLTLAGGKITDYRKMAEGAMETIINI
                     LDKEYNRKFKLINSKTYPVSGGEINPSNVDSEIEAYAQLGTLSGLSIEDARYIANLYG
                     SNAPKLFALTRQITEAEGLSLVETLSLHYAMDYEMALSPTDFFLRRTNHMLFMRDNLD
                     SLIQPVIDEMAKHYQWSDQDKTFYEEELHETLKDNDLAALKDN"
     gene            12266..12964
                     /locus_tag="VBE34_000070"
     CDS             12266..12964
                     /locus_tag="VBE34_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO7262831.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKNHNSGWIVITFGWG
                     LAVAIAALVSGNISPAHLNPAVSLAFAIKGDLAWGTAILYMIAQIIGAMLGSLLVYLQ
                     FRPHYEAAENKADILGTFATGPALKDNFSNFLSEVLGTLVLVLTIFAIGKYNMPPGVG
                     TMSVGMLVVGIGLSLGGTTGYAINPARDFGPRLLHALLPMKNKGDSDWTYSWIPIVGP
                     MVGAILAALIFAMM"
     gene            13052..14389
                     /locus_tag="VBE34_000071"
     CDS             13052..14389
                     /locus_tag="VBE34_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7262832.1"
                     /translation="MTEKYVNKVIVILGASFAGMTCAQKLRQLNPNWDIVLIDKEIHP
                     DYVPNGLNWYYRHEISGLNQAMWQTEEEQRLQNIRCLFGLKVEKINKEDRELMLSDGS
                     SVYYDQLICAMGSQAESTYIDGADAQGVLTTKTYATSQNAKQVLDKSHKVAVVGAGII
                     GLDIAYSLHESGKAVTLLEAQERPDFRHTDPDMSLPLLDAMAESKLHFFQNQKVEKIT
                     VTREEKLCLRTLTGDTFTVDTVILAVNFRPDSRLLTGLVDLSVDNSVVVNDYFQTSDP
                     NIYAIGDLIWSYFKGLNSAYYMPLINQAIRSAQMLAYHLSGHAVPKLKITRATGSKHF
                     GYYRANIGLTELEAGLYEDTVSVTYFPKEQYDLRIKLIANQKTGHLLGAQLISKENCL
                     ATANQLVQAISCDMTDFDLAFQDFIYTARESEMAYMLHQAAINLYEKRIGLCE"
     gene            14380..15810
                     /locus_tag="VBE34_000072"
     CDS             14380..15810
                     /locus_tag="VBE34_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7262833.1"
                     /translation="MRIEALMEKERRVQYRLLSFLRGSPQAIALKLALLETGLSRATL
                     LKYINNLNSYFEQEKVNCRIVYYKDKLFLEEDYNLSNQEVLKALMKDSIKYTILISLF
                     NQRQFTIVGLSQELMVSEATLNRHLAHLNELLAEFDIAISQGKQIGDELQWRYFYYEL
                     FKQLWSYDKCQNMIKKLDLDSLILLIERLAQHTLTREAQQNLGLWFSICHHRLLAMEK
                     LSDNLKPIVEHYQCNAFYKRLDAALVLYMSRFALEYREGEVLATFAFLHSQNILPINT
                     MEYIMGFGGPIIDCVTETIIYFKKESILADETSDQVIYQLGQLYSHYYFFKGHILVEQ
                     PDLEQTYRLIDHNMRDKLHDISKKIIANVNRIRPLTEDGCSLLTLHLLELLIFSKNSQ
                     KMPFRIGLDMTGNAVEQSLLEYRIRQHFSGNNSIQVEPYDEGKGFDMVIYQSRSRPYK
                     AKLTYCLNKGASERELQEIDSLIYDN"
     gene            15935..17920
                     /gene="tkt"
                     /locus_tag="VBE34_000073"
     CDS             15935..17920
                     /gene="tkt"
                     /locus_tag="VBE34_000073"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO7262834.1"
                     /translation="MTFDTIDQLAVNTVRTLSIDAIQAANSGHPGLPMGAAPMAYVLW
                     NKFLNVNPKTSRNWTNRDRFVLSAGHGSALLYSLLHLAGYDLSIDDLKQFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDLVDHYTYTLHGDG
                     CLMEGVSQEAASLAGHLKLGKLVLLYDSNDISLDGPTSQSFTEDVKGRFESYGWQHIL
                     VKDGNDLEAIAAAIEAAKAETDKPTIIEVKTIIGFGAEKQGTSSVHGAPLGAEGITFA
                     KKAYGWEYPDFTVPAEVAARFASDLQARGAKAEEAWNDLFAKYEVEYPELAAEYKEAF
                     AGQAETVELKAHDLGSSVASRVSSQQAIQQLSTQLPNLWGGSADLSASNNTMVAAETD
                     FQASNYAGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWQRAVSET
                     DRPTMLVLTRQNLPVLEGTSELAQEGVNKGAYILSEAKGELDGIIIATGSEVKLALDT
                     QDKLESEGIHVRVVSMPAQNIFDEQEASYQEQVLPSAVTKRLAIEAGSSFGWGKYVGL
                     NGLTLTIDTWGASAPGNRIFEEYGFTVENAVSLYKEL"
     gene            18132..18437
                     /locus_tag="VBE34_000074"
     CDS             18132..18437
                     /locus_tag="VBE34_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO7262835.1"
                     /translation="MKKECRDFYRQIQHTYNDISVREDAVLSSILLSASNGLIKTSDV
                     PRVAYELTQQLENNEIEKSFESLATVKELKKSAKKLSERYEQNKVTTGLLSKVFGNH"
     gene            18457..19191
                     /locus_tag="VBE34_000075"
     CDS             18457..19191
                     /locus_tag="VBE34_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7262836.1"
                     /translation="MIKFEHVSKVYGEKEALSDLTLSIKDGEIFGLIGHNGAGKTTTI
                     SILTSIIDATYGQVYIDDLLLTEHRDQIKKKIGYVPDSPDIFLNLTAEEYWYFLAKIY
                     DVAPEDIEARITKLVDIFELEEQRYNPIESFSHGMRQKVIVIGALLPNPDIWILDEPL
                     TGLDPQASFDLKEMMKEHAKNGKTVIFSTHVLAVAEQLCDRIGILKQGKLIFVGSLGE
                     LKMQYPDKDLETIYLELAGRQANREG"
     gene            19195..20799
                     /locus_tag="VBE34_000076"
     CDS             19195..20799
                     /locus_tag="VBE34_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7262837.1"
                     /translation="MNWSRIWELVKINILYSNPQTLSALRKKQEKHPKKEFSAYKSMF
                     RNQLFQILLFSIIYVFLFVSLDFKEYPGYFTFYIGIFTLVSIIYSFIAMYSVFYESDD
                     VKQYAYLPIKSEELYVAKIFATFGMSVTFLMPILTLMIVAYWRIIGGPLAVLLAIINF
                     AILFLSVTVISLYINSLIGRAIIRSANRKLISTILISLATFGAIVPLLFVNMTSQKMV
                     QGKLQDIAPISYVRGYYDIVTAPFSMESLLNYYLPLLIILFLIGAIYKWVMPRYYQEL
                     LYGQVKQRKVHRQIDFSKRESINKTLVKHHLSSLQNATLLTNTFLMPLLYLAMFIVPI
                     LNNGKEIGRFFNENYFGIAFLAGILIGSLCVMPASIVGVGISLEKSNFYFIKSLPISF
                     SYFLKHKFVTLITLQLAVPTFIYFLVGFFLLKLSILVLLSFILGLVFMGLIEGQFIYR
                     RDYKHLFLNWQEVTQLFNRGLGQWLLVGSLFGMMIIGSFLIGISIFWSMVWNTVAVNI
                     IILIIGLLILSICQYLLLKNFWKKLV"
     gene            complement(21007..22393)
                     /locus_tag="VBE34_000077"
                     /pseudo
     CDS             complement(21007..22393)
                     /locus_tag="VBE34_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643962.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            22534..23337
                     /gene="proB"
                     /locus_tag="VBE34_000078"
     CDS             22534..23337
                     /gene="proB"
                     /locus_tag="VBE34_000078"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEO7262838.1"
                     /translation="MKRHFETTRRIVIKVGTSSLVQTSGKINLSKIDHLAFVISSLMN
                     KGMEVILVSSGAMGFGLDILKMDKRPQEISQQQAVSSVGQVAMMSLYSQIFSHYQTHV
                     SQILLTRDVVVFPESLQNVTNSFESLLSMGILPIVNENDAVSVDEMDHKTKFGDNDRL
                     SAVVAKITKADLLIMLSDIDGLFDKNPNIYDDAVLRSHVSEITDDIIKSAGGAGSKFG
                     TGGMLSKIKSAQMVFDNNGQMILMNGANPRDILKVLDGHNIGTYFAQGK"
     gene            23347..24600
                     /locus_tag="VBE34_000079"
     CDS             23347..24600
                     /locus_tag="VBE34_000079"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEO7262839.1"
                     /translation="MTYIEILGQNAKKASQSVARLSTASKNEILRDLARNIVADTETI
                     LTENARDVAKAKDNGISEIMVDRLRLNKDRIQAIANGIYQVADLADPIGQVVSGYTNL
                     DGLKILKKRVPLGVIAMIFESRPNVSVDAFSLAFKTGNAIILRGGKDAIFSNTALVNC
                     MRQTLQDTGHNPDIVQLVEDTSHVVAEELMQATDYVDVLIPRGGTKLIQTVKEKSKIP
                     VIETGVGNVHIYIDEFADLDMAAKIVINAKTQRPSVCNAAEGLVVHQAIAKGFLSQLE
                     KMLKESNQSVEFRADEEALQLLENAVAASETDYATEFLDYIMSVKVVDSFEQAISWIN
                     KYSSHHSEAIITNNISRAEIFQDMVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKLH
                     ARGPMGLEALTSTKYYINGTGQVRE"
     gene            24683..25630
                     /gene="rsmH"
                     /locus_tag="VBE34_000080"
     CDS             24683..25630
                     /gene="rsmH"
                     /locus_tag="VBE34_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEO7262840.1"
                     /translation="MTNDFHHITVLLHETVDMLDIKPDGTYVDATLGGAGHSEYLLSQ
                     LGPDGHLYAFDQDQKAIDIAHIRLKKYVDTGQVTFIKDNFRNLSSNLKALGVSEINGI
                     CYDLGVSSPQLDERERGFSYKQDAPLDMRMNREQSLTAYDVVNTYSYHDLVRIFFKYG
                     EDKFSKQIARKIEQVRAEKPISTTTELAEIIKSSKSAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDLLAVDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLQPKMELVNRKPILPSQEELEANNRAHSAKLRVARRIR"
     gene            25645..25971
                     /gene="ftsL"
                     /locus_tag="VBE34_000081"
     CDS             25645..25971
                     /gene="ftsL"
                     /locus_tag="VBE34_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEO7262841.1"
                     /translation="MTNEKRTEAVTQTLQRHIKTFSRIEKAFYGAIVITAIIMAVGII
                     YLQSNSLQVKQEVNQLNSKINDKQTEFDNAKQEVNELSNRDRITKIAKDAGLTIQNDN
                     IYRKVD"
     gene            25975..28233
                     /locus_tag="VBE34_000082"
     CDS             25975..28233
                     /locus_tag="VBE34_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7262842.1"
                     /translation="MTFFKKLKKIFLDYVIHIRDRRSPQKNRERVGQNLMILTIFLFF
                     IFIINFVIIVGTDSKFGVNLSKEAKKVYQQSMTVQAKRGTIYDRNGNPIAEDATTYSL
                     YAIISKNYTTATGQKLYVQPSQYEKVASILENKLGMKKNLVLKQLNQKKLFQVSFGSS
                     GSGLSYTKMADIKKTMEKSDIKGIGFSTSPGRIYPNGIFASQFIGFTLPQDDGDGKKL
                     VGNTGLEAALNKVLSGTDGKVTYEKDRSGNVLLGTATTERRAVNGKDIYTTLSEPIQT
                     VLETQMDVFAEKTKGKFASATVVNAKTGEILATSQRPTYNPSTLKGYDKKNLGTYNTL
                     LYDNFFEPGSTMKVMTLASAIDSKHFNSTEVYNSAQYKIADAVIRDWDVNEGLSSGSY
                     MTFPQGFAHSSNVGMVTLEQKMGRDKWLNYLSKFKFGYPTRFGMLHESGGLFPSDNEV
                     TIAMSSFGQGIGVTQVQMLRAFTSISNDGVMLQPQFISSIYDPNTGTSRTARKEVVGK
                     PVSKEAASKTRDYMVTVGTDPYYGTLYAAGAPVIQVGNQSVAVKSGTAQIAQEGGGGY
                     LQGKNDTINSVVAMVPSENPDFIMYVTIQQPEKFSITFWKDVVNPVLEQATAMKETIL
                     KPVLNDSEHQTKYKLSKIVGENPGHVAEELRRNLVQPIILGNGSKVSKVSKRPGANLA
                     ENEQLLVLTNKLTELPDMYGWSKANVEQFAKWTGIKVTYKGSTSGKVRKQSIDVGKSI
                     NKIKKIKITIGD"
     gene            28235..29245
                     /locus_tag="VBE34_000083"
     CDS             28235..29245
                     /locus_tag="VBE34_000083"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEO7262843.1"
                     /translation="MFLSIMAGVIAFVLTVIAIPRFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLVVALLVSLIFSIILSKENSGNLGATFGILSVVLIYGIIGFLDDF
                     LKIFKQINEGLTPKQKMSLQLIAGLIFYFVHVLPSGTSAINIFGFNLEVGYLYAFFVL
                     FWVVGFSNAVNLTDGIDGLASISVVISLITYGIIAYNQTQFDILLIIVIMIGALLGFF
                     VFNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLFIGFVYVFETSSVMLQVAYFK
                     YTKKKTGVGKRIFRMTPFHHHLELGGVSGKGNKWSEWKVDAFLWAIGIFMSAITLAIL
                     YL"
     gene            29343..30686
                     /locus_tag="VBE34_000084"
     CDS             29343..30686
                     /locus_tag="VBE34_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7262844.1"
                     /translation="MSFKDFNFKPYIQRALDELKFVDPTDVQAKLIPVVRSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDESSDDVQVVITAPSRELATQIYQATKQIAEHSEQEIRV
                     VNYVGGTDKLRQIEKLKVSQPHIVIGTPGRIYDLVKSGDLVIHKAHTFVVDEADMTLD
                     MGFLDTVDKIAGSLPKDVQILVFSATIPQKLQPFLKKYLTNPVMEKIKTATVIADTID
                     NWLLSTKGRDKNAQILELSKLMQPYLAMIFVNTKERADELHSYLSSNGLKVAKIHGGI
                     APRERKRIMNQVKNLEFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLSGTAITLYQPSDDSDIRELEKLGINFIPKVIKNGEFQDTYDRDRRNNREKSYQK
                     LDTEMIGLVKKKKKKIKPGYKKKIQWKVEEKRRKERRASNRAKGRAERKAKKQSF"
     gene            30824..31636
                     /locus_tag="VBE34_000085"
     CDS             30824..31636
                     /locus_tag="VBE34_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7262845.1"
                     /translation="MFKKIILSIATIAATASLAVSVQASEKVELKVATDSDTAPFTYQ
                     KDGKFKGYDVDVVKAVFKGSKYKVTFKTVPFDTISTGIDAGKFDLSANDFSYNKERAE
                     KYLFSDPISRSNYAVVGKKGSHYKSLSDLSGKSTEVLSGVNYAQVLENWNKNHPNKKP
                     IKIKYVSGTTGVTSRLKNIESGKIDFILYDAISSDYIVKDQSLNLSVSPLKGKIGNNK
                     DGLEYLLLPKDKKGKTLQKFINKRIKVLKEDGTLARLSKQYFGGDYVSNIDK"
     gene            31731..32534
                     /locus_tag="VBE34_000086"
     CDS             31731..32534
                     /locus_tag="VBE34_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7262846.1"
                     /translation="MNQFILTGGWSWYNNLVSQVPAGKLFSWKAVLDAIPSILERLPI
                     TLLLTVAGALFGLILALIFAVVKINRVKILYPIQALFVSFLRGTPILVQLMLSYYGIP
                     LFLKFLNQKYGFDWNINAIPASVFAITAFAFNEAAYTSETIRAAILSVDQGEIEAARS
                     LGMTSAQVYRRVIIPNAAVVATPTLINTLIGLTKGTSLAFNAGIVEMFAQAQIMGGSD
                     YRYFERYISVALVYWAVSFLIEQLGNAIERKMAIKAPHHLTDEIPGGVR"
     gene            32534..33277
                     /locus_tag="VBE34_000087"
     CDS             32534..33277
                     /locus_tag="VBE34_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7262847.1"
                     /translation="MIKLRQLTKSFSGQKVLDKLDLDIEKGQVVALVGASGAGKSTFL
                     RSMNYLEEPDYGTIEIDDFKVDFKSISKDDILTLRRKLAMVFQQFNLFERRTALDNVK
                     EGLKIVKKMSDQEATRIARDELAKVGLADREKYYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKQGQTMVLVSHDMNFVYQVADKVLFLEKGRIL
                     ESGTPEQLFNHPLEERTKEFFASYNKSYL"
     gene            33399..33623
                     /locus_tag="VBE34_000088"
     CDS             33399..33623
                     /locus_tag="VBE34_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEO7262848.1"
                     /translation="MFLEIAKVYLQGLGLASITVILVGIFWVFWRAKKNIDKTARERQ
                     SVLYDVLLIALMTAPILAFAFVAIILMIKA"
     gene            33692..34606
                     /gene="trxB"
                     /locus_tag="VBE34_000089"
     CDS             33692..34606
                     /gene="trxB"
                     /locus_tag="VBE34_000089"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEO7262849.1"
                     /translation="MYDTLIIGSGPGGMTAALYAARSNLKVGLIEQGAPGGQMNNTAE
                     IENYPGYDHISGPELSMKMYEPLEKFEVEHIYGIVQRVENDGDVKRVITEDESYEAKT
                     VILATGAKNSLLGVPGEEEYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAVFLT
                     QFAKSVTIIHRRDQLRAQKVLQDRAFANEKIKFVWDSVVKEIKGNEIKVSGVTVENLK
                     TGEISEMTFGGVFIYVGLKPHSSMVSELGITDETGWVLTDTNMKTSIPGLYAIGDVRQ
                     KDLRQIATAVGEGAIAGQGVYNYITEHF"
     gene            34764..36224
                     /locus_tag="VBE34_000090"
     CDS             34764..36224
                     /locus_tag="VBE34_000090"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEO7262850.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFNKGIHNKKAVFEAYFRKVPFE
                     NGYAVFAGLERIVRYLENLSFSDSDLSYLEELGYPEEFLDYLKNLKMELTVKSAKEGD
                     LVFANEPLVQIEGPLAQCQLVETAILNIINYQTLVATKAARIRSVIEDEPLLEFGTRR
                     AQEMDAAIWGTRAAIIGGANATSNVRAGKIFNIPVSGTHAHALVQTYGDDYQAFKAYA
                     ETHKDCVFLVDTYDTLRVGVPNAIRVAKEMGEKINFLGVRLDSGDLAYLSKKVRQQLD
                     DAGFPNAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     TDAGSMRDTIKLSNNAEKVSTPGKKQVWRITSRAKGKSEGDYITFADTDVTQLDEIDM
                     FHPTYTYINKTVRDFDAVPLLVDIFDKGKLVYQLPSLQEIQEYGRKEFDQLWDEYKRV
                     LNPQDYPVDLARDVWQNKMDLIDRIRKEALAKGEVR"
     gene            36221..37042
                     /gene="nadE"
                     /locus_tag="VBE34_000091"
     CDS             36221..37042
                     /gene="nadE"
                     /locus_tag="VBE34_000091"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEO7262851.1"
                     /translation="MTLQDQIIKELGVKPVINPSQEIRRSVEFLKDYLLKHSFLKTYV
                     LGISGGQDSTLAGRLAQLAVEELRADTGENYQFIAIRLPYGIQADEEDAQKALDFIKP
                     DIALTINIKEAVDGQVRALNAAGVEITDFNKGNIKARQRMISQYAVAGQYAGAVIGTD
                     HAAENITGFFTKFGDGGADLLPLFRLNKSQGKQLLAELGADKDLYEKIPTADLEENKP
                     GIADEIALGVTYQEIDAYLEGKVVSDKSRGIIENWWYKGQHKRHLPITIFDDFWK"
     gene            37155..38489
                     /gene="pepC"
                     /locus_tag="VBE34_000092"
     CDS             37155..38489
                     /gene="pepC"
                     /locus_tag="VBE34_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEO7262852.1"
                     /translation="MSELTQTFTDKLLADYQANTKFSAIENAVTHNGLLKSLETRQSE
                     IENDYVFSIDLTKDEVSNQKQSGRCWMFAALNTFRHKLISDFKLENFELSQAHTFFWD
                     KYEKSNWFMEQIIATANQELSSRKVKFLLDVPQQDGGQWDMVVALFEKYGVVPKTVYP
                     ESVSSSASRELNQYLNKLLRQDAQILRELIAQGADGATVQNKKEELLQEIFNFLAMNL
                     GLPPQSFDFAYRDKDNHYQSDKNITPKAFYQKYVNLDISDYVSIINAPTVDKPYGQSY
                     TVEMLGNVVGGPAVKYLNLDMKRFKELAIAQMKSGETVWFGSDVGQVSNRQKGILATT
                     TYDFNSSMDIKLSQDKAGRLDYSESLMTHAMVLTGVDLDESGQPLKWKVENSWGEKVG
                     KDGYFVASDAWMDEYTYQIVVRKELLTKEELEAYNAEPITLAPWDPMGALAN"
     gene            complement(38536..40776)
                     /locus_tag="VBE34_000093"
     CDS             complement(38536..40776)
                     /locus_tag="VBE34_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7262853.1"
                     /translation="MITIKKESVIKLLKYAFGIIMGFIILAIVIGGLLFAYYVSRSPK
                     LTDQALKSVNSSLVYDGNNKLIADLGSEKRESVSADSIPLNLVNAITSIEDKRFFKHR
                     GVDIYRILGAAWHNLVSSNTQGGSTLDQQLIKLAYFSTNKSDQTLKRKSQEVWLALQM
                     ERKYTKEEILTFYINKVYMGNGNYGMRTTAKSYFGKDLKELSIAQLALLAGIPQAPTQ
                     YDPYKNPESAQTRRNTVLQQMYQDKNISKKEYDQAVATPVTDGLKELKQKSTYPKYMD
                     NYLKQVISEVKQKTGKDIFTAGLKVYTNINTDAQKQLYDIYNSDTYIAYPNNELQIAS
                     TIMDATNGKVIAQLGGRHQNENISFGTNQSVLTDRDWGSTMKPISAYAPAIDSGVYNS
                     TGQSLNDSVYYWPGTSTQLYDWDRQYMGWMSMQTAIQQSRNVPAVRALEAAGLDEAKS
                     FLEKLGIYYPEMNYSNAISSNNNSSDAKYGASSEKMAAAYSAFANGGTYYKPQYVNKI
                     EFSDGTNDTYAASGSRAMKETTAYMMTDMLKTVLTFGTGTKAAIPGVAQAGKTGTSNY
                     TEDELAKIEATTGIYNSAVGTMAPDENFVGYTSKYTMAIWTGYKNRLTPLYGSQLDIA
                     TEVYRAMMSYLTGGYSADWTMPEGLYRSGSYLYINGTTTTGTYSSSVYKNIYQNSGQS
                     SQSSSSTSSEKQKEDKNTADDANSSSPQVETPNNGNATTPNNSNQTVPGTGHGNGNNN
                     TAPNGN"
     gene            complement(40763..41362)
                     /gene="recU"
                     /locus_tag="VBE34_000094"
     CDS             complement(40763..41362)
                     /gene="recU"
                     /locus_tag="VBE34_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEO7262854.1"
                     /translation="MVNYPHQLIRKTTVTKSKKKKIDFANRGMSFEAAINATNDYYLS
                     HELAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYSGVYKGYYIDFEAKETR
                     QKTAMPMKNFHAHQIEHMANVLQQKGICFVLLHFSTLKETYLLPANELISFYQIDKGN
                     KSMPIDYIRKNGFFVKESAFPQVPYLDIIEEKLLGGDYN"
     gene            41437..41955
                     /locus_tag="VBE34_000095"
     CDS             41437..41955
                     /locus_tag="VBE34_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEO7262855.1"
                     /translation="MKSTILVTGYKNFELGIFQDKDPRITIIKKAIDKDFRRFLENGA
                     DWFIFMGNLGFEYWALEVALDLQKEYDFQIATIFTFENHGQNWNEANKAKLALFKQVD
                     FVKYTFPSYENPGQFKQYNHFLINNTQGAYLFYDSENETNLKFLLEMMEKKEAYDISF
                     LTFDRLNEIYEE"
     gene            42076..42408
                     /gene="gpsB"
                     /locus_tag="VBE34_000096"
     CDS             42076..42408
                     /gene="gpsB"
                     /locus_tag="VBE34_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEO7262856.1"
                     /translation="MASIIYSPKDIFEQDFKVSMRGYDKKEVDVFLDDVIKDYENYLE
                     QIEKLQMENRRLQQALDKKESEASNVRNSGTAMYNQKPIAQSATNFDILKRISRLEKE
                     VFGRQIRE"
     gene            42427..42795
                     /gene="rnpB"
                     /locus_tag="VBE34_000097"
     ncRNA           42427..42795
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VBE34_000097"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            42878..44032
                     /locus_tag="VBE34_000098"
     CDS             42878..44032
                     /locus_tag="VBE34_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEO7262857.1"
                     /translation="MKESFKLIATAAAGLEAIVGREIRNLGIDCQVENGRVRFHGDIK
                     TIIETNLWLRAADRIKIIVGEFPARTFEELFQGVYGLDWENYLPLGAKFPIAKAKCVK
                     SKLHNEPSVQAISKKAVAKKLQKVFHRPEGVPLQENGAEFKIEVSILKDKATVMIDTT
                     GSSLFKRGYRAEKGGAPIKENMAAAIIQLSNWFPDKPLIDPTCGSGTFCIEAAMIGMN
                     IAPGFNRDFAFEAWPWVDQSQVQKVRDEAESKANYDIDLDISGFDLDGRMVEIARKNA
                     EEAGLGDVIKLKQMRLQDLKTDKINGVIISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDEGFEKKYGSQADKKRKLYNGTLKVDLYQYYGERVRRQVK"
     gene            44045..45532
                     /locus_tag="VBE34_000099"
     CDS             44045..45532
                     /locus_tag="VBE34_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262858.1"
                     /translation="MSEDQKHPFFEPEKETDGLEFKDAKEMTVEEAVRKDSEIKAGIT
                     EEDSILDKYIKQHRDEVASQKFETKSSDFANLDTASLDDFIKKQREELSAMLAAEELS
                     KKLDNSVSQEQDIEANAVSPKEESSQEQENSVTPVPPLNTEAEPTATEPDSTIADSEE
                     YKSSSKKRGGIVGTLIALILLLIVTIFGYNYFKNNNSTNSQTATSQSSSSKATTTSSE
                     EDKKASQNLDNFNKAYANFFVDDKKTQLKNSEFDKLSELEKKVDALKGTKYYGKVKVK
                     FDSLKRQIDAVKAVNDKFKSPAVVDGKKSEKLEVKDGANFDSLDSKTLNTGNASLDSL
                     LHSIVSTGRNQVKQSEEQAKKTQEEQSSSNKVSDTQTTEQPNVTNGQSSSSAATINNQ
                     AAGTASGNLERNRSRVPYNNAAIADTGNPAWIFNPGVLEKIVATSQARGYFSGNNYIL
                     EPVNIINGNGYYNMFKLDGTYLFSINAKTGYFVGNAPGRADSLDY"
     gene            complement(45757..46239)
                     /locus_tag="VBE34_000100"
     CDS             complement(45757..46239)
                     /locus_tag="VBE34_000100"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEO7262859.1"
                     /translation="MTKEVVVESFELDHTIVKAPYVRLISEEVGPVGDIITNFDIRLI
                     QPNENAIDTAGLHTIEHLLAKLIRQQINGLIDCSPFGCRTGFHMIMWGKQDATEIAKV
                     IKSSLEAIAGGVTWEDVPGTTIESCGNYKDHSLHSAQEWAKLILSQGISDNAFERHIV
                     "
     gene            46428..48035
                     /locus_tag="VBE34_000101"
     CDS             46428..48035
                     /locus_tag="VBE34_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEO7262860.1"
                     /translation="MFNIILAMVCALIGLIIGYVAISMKMKSSKEAAELTLLNAEQDA
                     VDLRGKAEIEAEHIRKAAERESKAHQKELLLEAKEEARKYREEIEKEFKSDRQELKQM
                     EARLTDRASSLDRKDENLSNKEKMLDSKEQSLTDKSRHINEREQEIATLETKKVEELS
                     RIAELSQEEAKDIILADTEKDLAHDIATRIKEAEREVKDRSNKIAKDLLAQAMQRLAG
                     EYVTEQTITTVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVVLSGFDPI
                     RREIARMTLESLIQDGRIHPARIEELVEKNRLEMDQRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSYGQNVLRHSVEVGKLAGILAGELGENVDLARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPIVVNTIASHHGDVEPDSVIAVIVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIANGFEGVQNAFALQAGREIRIMVQPGKVSDDQVVIMSHKVRE
                     KIEQNLDYPGNIKVTVIREMRAVDFAK"
     gene            48164..49060
                     /locus_tag="VBE34_000102"
     CDS             48164..49060
                     /locus_tag="VBE34_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7262861.1"
                     /translation="MAKDIIKVDHIFKSIGQKTILEDISFSIASNQCVALIGPNGAGK
                     TTLMSTLLGDISISSGALTIFNLPAHHNRLKYKVAILPQENVLPSKFTVRELIDFQRC
                     LFPEVLPMSLILDYLQWSDAHLQQFTETLSGGQKRLLAFVLTLVGKPQLLFLDEPTSG
                     MDTSTRQRFWELVATLKKEGDTIVYSSHYIEEVEHTADRILVLHKGKLLRDTTPFAMK
                     QEKTEKLFTVPLSYQKLLPTYLITECEAKSDSITFVTGEAETVWKILADNGCPIEAIE
                     MTNRTLLNRIFETTKEVKHENL"
     gene            49047..49787
                     /locus_tag="VBE34_000103"
     CDS             49047..49787
                     /locus_tag="VBE34_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7262862.1"
                     /translation="MRIFKALLKMEFLLTKRQLANLIMAIGMPVAFFLFFSGFMGEGL
                     TKAIEAIYVRNYMITMAGFSSLSFAFFTFPFSMKDDQLSNRMQLLRHSPVPMWQYYLA
                     KIIRILFYYCLAITVVFLTGHILRQVSMPIEQWMQSFLLLLGGATCFIPFGLLVSYFK
                     NTELMSMVANICYMSLAVLGGMWMPITMFPKWLQGLSKLTPTYHLTQVILSPFANSFA
                     VFSLIILIGYGIIMLVIAYLLSQKRHSI"
     gene            49784..50869
                     /locus_tag="VBE34_000104"
     CDS             49784..50869
                     /locus_tag="VBE34_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7262863.1"
                     /translation="MKNKKNNYDLMYFIPLVFLIYPIGGILYYHYPFWTLFFTLAFVG
                     AYLYSIIIRGESKYHMIAWFTMLTYIFYMTVFINSGFIWYIYFLSNLLVYRFRDKLKS
                     FRFISFACTLATMVFLCFFKASDFGDRIMFLIVPIFCIGYMWIAIENRNSEEQREKIA
                     EQNQYINILSAENERNRIGRDLHDSLGHTFAMMTLKTELALKLLEKRNYDKVQKELSE
                     LNHISHQSMSEVRQIVSNLKYRTVVEEIDELYRLFQLSNIKLTVVNKLETSQLSPVTQ
                     STITMILKELSNNIVKHAEADSVELSLVRQGATINIEMIDNGCGFTNLDGDELHSIQE
                     RLTIVEGTLTILSRSKPTHIQVILKEV"
     gene            50871..51461
                     /locus_tag="VBE34_000105"
     CDS             50871..51461
                     /locus_tag="VBE34_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7262864.1"
                     /translation="MKLLVAEDQSMLRDAMCQLLLMEESVSTIDQAGNGEEAIAILSN
                     KAIDVAILDVEMPILSGLDVLEWVRKYQNVKVIIVTTFKRSGYFQRAIRSNVDAYVLK
                     DRSVADLMKTIQKVLSGGKEYSPELMENVISNPLSKQEIKILSLIAQGKTSKEIACTL
                     FLSNGTVRNYTSNIFDKLGVNNRLEALKIAREKDWL"
     gene            51511..52215
                     /locus_tag="VBE34_000106"
     CDS             51511..52215
                     /locus_tag="VBE34_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEO7262865.1"
                     /translation="MQKFKERFDTLSDAILAIAMTILVLEIKTPTTMGDIGDFTRNIG
                     LFIVSFVVVFNFWYERAQNSLDAQKTNDEIIALDIIEHLGICLIPLFTKFMISFENHN
                     FAVMAYGLLTLLVGLTSDIIRIRLASYDLVTIPSELKERVIKVMTTFAIRSVVVRFII
                     IILAYFLPEVGIFAYLVIPLFMFWRRSRAGKYYTKKGIEAPSYIKLFIQIGERVPEDF
                     TNSSSGISGVKRLYKK"
     gene            52382..53011
                     /gene="gmk"
                     /locus_tag="VBE34_000107"
     CDS             52382..53011
                     /gene="gmk"
                     /locus_tag="VBE34_000107"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEO7262866.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFDYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEALIKEGQMLEYAEYVGNYYGTPLSYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFIFLTPPDLEELEERLVGRGTDSPEVIAQRIERAKEEIALM
                     REYDYAVVNDQVSLAAERVKRVIEAEHYRVDRVIGRYTNMVKETDKKLS"
     gene            53034..53348
                     /locus_tag="VBE34_000108"
     CDS             53034..53348
                     /locus_tag="VBE34_000108"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEO7262867.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGEKATQDFKSV
                     KSTLRALEEIESGNVVIHPDPSAKRASVRARIEAERLAKEEEERKIKEQIAKEKEDGE
                     KI"
     gene            53422..55812
                     /locus_tag="VBE34_000109"
     CDS             53422..55812
                     /locus_tag="VBE34_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEO7262868.1"
                     /translation="MARKLAQVIVDIPLMQTDKPFSYAIPKDLEDLVQVGARVHVPFG
                     RGNRLLQGFVVGFRDDDELETKDIAGVLDFEPVLNQEQLDLADQMRHTVFSYKISILK
                     SMLPSLLNSQYDKLLLATDTLPSEDREDLFGHKTEIVFSSLSSQDAKKAGRLIQKGFI
                     EVQYLAKDKKTIKTEKIYKINRTLLEESQIAARAKKRLELKEFLLENPQPGRLTALNK
                     QFSSPVVNFFREEGIIEVIEKEASRSDNYFKGILKTDFLDLNQEQAKVVKIVVDRIGK
                     DQNKPFLLEGITGSGKTEVYLHVIDNVLKLGKTAIVLVPEISLTPQMTNRFISRFGKR
                     VAIMHSGLSDGEKFDEWRKIKSGQAKVVVGARSAIFAPLENIGAIIIDEEHESTYKQE
                     SNPRYHARDVALLRAEYHKAVLLMGSATPSIESRARASRDVYKFLELKHRANPKARIP
                     QVEIIDFRNFIGQQEVSNFTPYLLDKIRDRLDKKEQVVLMLNRRGYSSFIMCRNCGYV
                     DQCPNCDISLTLHMATKTMNCHYCGFEKPIPRTCPNCNSKSISYYGTGTQKAYEELLK
                     VIPDAKILRMDVDTTRQKGGHESILKRFGNHEADILLGTQMIAKGLDFPNVTLVGVLN
                     ADTSLNLPDFRSSERTFQLLTQVAGRAGRAEKEGEVVIQTYNPNHYAIQLAQKQDFEA
                     FYQYEMNIRRQLGYPPYYFTVGLTLSHKDEEWLIRKSYEVLSLLKQGFSDKVKLLGPT
                     PKPIARTHNLYHYQIIIKYRFEDNLELVLNRLLDITQDKENRDLRLAIDHEPQNMM"
     gene            55859..56794
                     /locus_tag="VBE34_000110"
     CDS             55859..56794
                     /locus_tag="VBE34_000110"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEO7262869.1"
                     /translation="MTKLLFMGTPDFSATVLKGILADGKYDVLAVVTQPDRAVGRKKE
                     IKMTPVKEVALENNIPVYQPEKLSGSPELEQLMTLGADGIVTAAFGQFLPTKLLESVG
                     FAINVHASLLPKYRGGAPIHYAIINGEKEAGVTIMEMVAKMDAGDMVSKASVEITDED
                     NVGTMFDRLAVVGRDLLLDTLPGYLSGDIKPIPQNEEEVSFSPNISPDEERIDWNKSS
                     RDIFNHVRGMYPWPVAHTLLEGNRFKLYEVTMSEGKGSPGQVIAKTKNSLTVATGDGA
                     IELKSVQPAGKPRMDIKDFLNGVGRNLEIGDKFGE"
     gene            56784..58106
                     /gene="rsmB"
                     /locus_tag="VBE34_000111"
     CDS             56784..58106
                     /gene="rsmB"
                     /locus_tag="VBE34_000111"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEO7262870.1"
                     /translation="MANDWKKSARGLALMTLEEVFDKGAYSNIALNKSLKKSRLSDKD
                     RALVTEIVYGTVARKITLEWYLSHFIVDRDKLELWVYHLLLLSLYQLLYLDNIPDHAI
                     VNDAVTIAKNRGNKKGAEKLINAVLRRVSSETLPEIASIKRQNKRYSVAYSMPVWLVK
                     KLIDQYGETRALAIMESLFERNKASLRVTDLSQKQTIKETLNVRDSHIAETALVADSG
                     NFASTSFFQDGLITIQDESSQLVAPTLKVSGNDQVLDACSAPGGKTSHIASYLTTGAV
                     TALDLYDHKLELVMENAKRLGLSDKIKTKKLDASKAHEYFPEDTFDKILVDAPCSGIG
                     LIRRKPDIKYNKANQDFEALQEIQLSILSSVCQTLRKGGIITYSTCTIFEEENFQVIE
                     KFLENHPNFEQVELSHTQEDIVKRGCISISPEQYHTDGFFIGQVKRIL"
     gene            58144..58881
                     /locus_tag="VBE34_000112"
     CDS             58144..58881
                     /locus_tag="VBE34_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEO7262871.1"
                     /translation="MEISLLTDIGQRRSNNQDFINQFENKAGVPLIILADGMGGHRAG
                     NIASEMTVTDLGSDWAETDFSELSEIRDWMLVSIETENRKIYELGQSDDYKGMGTTIE
                     AVAIVGDNIIFAHVGDSRIGIVRQGEYHLLTSDHSLVNELVKAGQLTEEEAASHPQKN
                     IITQSIGQANPVEPDLGVHLLEEGDYLVVNSDGLTNMLSNADIATVLTQEKTLDDKNQ
                     DLITLANHRGGLDNITVALVYVESEAV"
     gene            58881..60836
                     /gene="pknB"
                     /locus_tag="VBE34_000113"
     CDS             58881..60836
                     /gene="pknB"
                     /locus_tag="VBE34_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEO7262872.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLARDLILDNEEVAIKVL
                     RTNYQTDQIAVARFQREARAMAELTHPNIVAIRDIGEEDGQQFLVMEYVDGFDLKKYI
                     QDNAPLSNNEVVRIMNEVLSAMSLAHQKGIVHRDLKPQNILLTKKGTVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSILAENKSVPQALENIVIKATAKKLTDRYKTTYEMGRDLSTALSST
                     RHREPKLVFNDTESTKTLPKVTSTVSSLTTEQLLRNQKQAKTTEKITPDSASNDKTKS
                     KKKASHRLLGTIMKLFFALCVVGIIVFAYKILVSPTTIRVPDVSNKTVAQAKMTLENS
                     GLKVGAIRNIESDSVSEGLVVKTDPAAGRSRREGAKVNLYIATPNKSFTLGNYKEHNY
                     KDILKDLQGKGVKKSLIKVKRKINNDYTTGTILAQSLPEGTSFNPDGNKKLTLTVAVN
                     DPMIMPDVTGMTVGEVIETLTDLGLDADNLVFYQMQNGVYQAVVTPPSSSKIASQDPY
                     YGGEVGLRRGDKVKLYLLGSKTTNNSSSTPIDSSASSSTGTTTSDSVSSSTDASTSDS
                     SSTSTSSSTLPSDSTINTGTANNPLTQ"
     gene            60995..>61270
                     /locus_tag="VBE34_000114"
     CDS             60995..>61270
                     /locus_tag="VBE34_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEO7262873.1"
                     /translation="MKKFQFFLLVEAVVLVMGLMKILSDDWTSFIFILALILLALRFY
                     NNDSRHNFLLTTSLLLLFLIFMLNPYIIAAVVFAVLYVLINHFSQVKK"
BASE COUNT        19997 a         9611 c        12401 g        19261 t
ORIGIN      
        1 tttttgctcc tctctcagtg agtgagtgtt tactcaccaa tttgatatca tatattatag
       61 tgtgtaaaga caggcttgtc aataaaagaa ttgacaggaa gttaatatac tataatagtc
      121 aataaaatgg cttatttgag ataactaatt ttttatctta aaaatcagtt gcatggagag
      181 cattcgtgaa aatgggcaca gaataaaacg actaagaata tccttgtcgt tttatttttt
      241 atatagttat acctctttta aaattcaata taataatgtt tattcaggta ttttttaaca
      301 atgatatgaa gagagaagct aagtcccagt ataattaaat aaatccaaag ggggatatta
      361 atgaaaggaa gatgacaata ttttcctata aggatataag ctatgataag cgaaagaatt
      421 agaaacatat atcgaaagaa atatttcttt tctttcgatt tatcgaagtt atatgtagca
      481 tatattatgg tatcagttat cttgacagca ataatactga taaagattgc aaaaagaaca
      541 tctaatagag taatagggat tagatctggt actgactgtt gaagtgcttc agtaatgaga
      601 tatcctccta aaaagataat catttggata gttagtagta aaatggctaa atcccaacgt
      661 tgtaatgttt cttctttctt agagatttta ggtgtagatt taataacttg gtttataaag
      721 tcgtgcctat cttctccaaa tacactaact aaacttgctt gtcgctcttg tgcttctaat
      781 aacatattga caatatcatt tcgaatgagc tcctggtggt aggggctaat gctagcacct
      841 cgcaaataga caatcatatc tgtataggtt tggttattag agttatcaat tcttccttct
      901 aatagcgtat tttctttttg taattttttc agtttatcca tgctagtttc ctctccaatt
      961 tttagagtag tttgtttaat gctttactaa gtgactccca attctgttta aattgggcta
     1021 attgctccac gccttcattg gttaacgtat aatatttacg tttaggtcct tgaggggaag
     1081 gcttttgttg tccttttata tatttattgc tttgtagcct taagataatg gggtagattg
     1141 tcccttcgga aatttcccca aaaccataag aatttaattt ttttgagatt tcataagcat
     1201 aagtctcctc ttgctgtatg atttttagga tagctccttc aagtaatcct tttagcattt
     1261 gcgatggaat catattatat ccttatctat attgtaatac aagttactgg ttaactacat
     1321 tgtaatacaa agtagttgag atgtcaaata taactaagtt accggtagaa aataagagtg
     1381 atagacgatg attcttctaa aataggtttg tttatgtcct ttagtttatg ttataataat
     1441 gtgaagaaac tttaggagtg agtatgaaaa tagataaaaa agaactttta gcacttattg
     1501 ctagtattat tttattaatt tttgcttctg ttacgttttt cttatttaaa gatcatggca
     1561 ctactcaaat ggatactgta gaaagcagtg ttaaccatgt cagtgattct caattaactg
     1621 aggcacaaga tatgttagat aaatttgaga agaaaccttc agaaaaatta ttgaaggatg
     1681 tcgaacttgc tttaaataaa ttgtccaatt cttctaaaaa agaagctttg caaaaacgtt
     1741 ttaagaaagc aaaagataag tatcttaaag acgaagcaga taagaaagct accaaagatg
     1801 ctacagattt agtagaaatt ctagaacaag ccccttcaga agaaaatgtg ttaaaagctg
     1861 aagcagcagt caaaaagtta actgtcaaag aatctaaaga agctttacaa aaacgtattg
     1921 atactgttaa gacacaatat ggtttgattg ggaatcaaac tccctctagt tcagtagctg
     1981 aaactacaga acaagggaca gctaatcctg ctagtcagga tacttctagt tacgttaatc
     2041 aggatgtagc accaacttat gagcaaccgc aaacgaataa tacaccagtt actccagggg
     2101 ttaacaatac tgttccgact ccaggaactg gtactgtacc tgctactaat gggacgggtg
     2161 ttgctcagta aaatcctgaa agtctagttt tactagactt ttttgctatg ctttttaatt
     2221 agccattctc atcaaaaact ataagagaat tagcaattga tagttttgga gaaggaaaag
     2281 aattatgagc aacagcaatt aaaaccttaa aagtacaacc aagtgaagtg gtttataaca
     2341 agtgtatagt tctttcttag aataaaccat gactttttaa aaaataggta tataccattt
     2401 acaaaaatac cttaaagcgt tataatgaga gtgaatgggg ggaagaagat gacgaagtac
     2461 atcaaggctg accgattctt ttatgctgat catgtaaagg aaaacggtta tttagaaata
     2521 aaagataatc actttggtaa atggatagaa aatatttctg gacaagagga gattttagat
     2581 tattcaggtt accaaatagc acctggttta gttgatacac atattcatgg ttttgctggc
     2641 gctgatgtta tggattgtga ttcagaagga attttgcgaa tgagtgctgg cttactatct
     2701 actggagtca catctttctt gccgacaacc ctaacttcag atacaaagcg actagaagag
     2761 gcgtctaaag cagttgcggc agtagcgggc aaagaacaag gagcaaaaat tcaaggcatc
     2821 tattttgagg gaccatattt tactgaggaa tataaaggag ctcaaaatcc gatttatatg
     2881 agaaatccaa acttggaaga gtttgctcaa tggcagaagg ctgcaaaagg cttgattaca
     2941 aaaattgctt tggcaccaga acgtgaggga gttgaggaat ttgtctcagc aatcacaaag
     3001 caaggtgtta cggtagcttt aggtcattct aatgggacat ataaagaagc taaaaaggca
     3061 gtgaaggcgg gtgcctcagt ttgggtacat gcgtataatg gtatgcgtgg tttgacacac
     3121 cgagaaccag gtatggttgg tgcggtttat aatttaccta atacttatgc tgaactcatt
     3181 tgcgatggtc atcacgttga tcctgttgct tgtgacattt taatgacaca aaaaggccat
     3241 aatcatgtgg cattaataac agattgtatg gcagctggag gtgcccctga tggagattat
     3301 atgttagggg aattgcccgt ggttgtgtct aatggaactg cgcgtttgaa agatggaggg
     3361 aatttagctg gttctattct taagctcaaa gatggattaa aaaatgttgt agaatgggga
     3421 attgctactc cggaagaggc agtgcgtatg gcgagttatg ttccagcagt gtctgtaggc
     3481 attgataatg tctgtggaca gataaaagaa ggctatgcag cggattttat tgtattagat
     3541 aaacatctag atttagttgc aacatacctt gatggagaaa aacgttggca atcttgaaag
     3601 aactagtttt tactagttct ttttaattta ttaattttta atgaaatata gtttgacaat
     3661 aaaactattt ttcttataat gttgtaatat aatacaaaac aataggagtt tacaatgtca
     3721 attattattg ggttaatggc cgctatgttt atcattcgtt tagcttattt aaagctatct
     3781 atagctaatg aaaaggcctt aagaaaaaat ggtgctaaag aatatggtgt aggtgtttca
     3841 aaagcaatca cagttcttca tattatcatt tatttttcat ctgtaactga ggctgttcta
     3901 acaaaagcaa gttttaattt tgttagcgta atcgggctta gcttgatgat tttttctgtg
     3961 tttatgttac atacagtaac acgtttattt ggtcgtattt ggacagtgaa attaatggta
     4021 gataaaaatc atcaatttgt agatcactgg ctatttaggg ttgttaagca tcctaactat
     4081 ttcttaaata ttgctcctga acttttaggt gtaactttat tgtgtcatgc taagtataca
     4141 gcattatttg tcttgccaat ttatgctttt gttatttatt taagaattcg tgaagaaaac
     4201 ttattattaa aaacgatcat tattccaaat ggaatcaaaa agtctagggt atcttaagaa
     4261 taagataaat agtagacata aaagtcattt tttgttatac tgtctcagta agaagaggtg
     4321 ttcatcagct gtttttttag cgagctcaag gtagtgaaag ttgggtagaa taggatgaat
     4381 attggcactt tttagtgagt aaaagtacaa tcaaaaatga agtaataaat tagggtggaa
     4441 ccgcgtcttt gacgcccctg tgctgttggg cataggtggt gtaggacgtg gtttttatca
     4501 tttgtccgtt ttaagtcaat tgttgtcacc ttcccaacag ctgtaataaa gtatgaaaga
     4561 aggaaaatga tgtctaagaa attaactttt caagaaatta ttctaacttt acaacagttt
     4621 tggaatgacc aaggctgtat gttaatgcaa gcttatgata atgagaaagg agcagggaca
     4681 atgagccctt atactttctt acgtgctata ggccctgagc cttggaatgc tgcatacgtt
     4741 gaaccatcac gtcgtccagc tgatggccgc tatggtgaaa atccaaaccg tctttaccaa
     4801 catcatcaat ttcaagtggt aatgaagcct tcgccgtcaa atattcaaga actttatttg
     4861 aagtctttgg aattactagg tatcaatcca cttgagcatg atatccgttt tgttgaagat
     4921 aactgggaaa atccatctac aggttcagct ggattaggtt gggaagtttg gcttgatgga
     4981 atggaaataa cacaatttac ttatttccaa caagtaggtg gacttcaaac aggtcctgta
     5041 acttctgaag ttacttatgg actagagcgt ttagcctctt atattcaaga ggtagattct
     5101 gtttatgata ttgagtgggc accaggtgtt aaatatggtg aaatctttac tcagccagag
     5161 tatgagcatt caaaatattc atttgagata tcagaccaag taatgttact tgaaaacttt
     5221 gagaaatttg aaagagaagc aaaacgtgct ttagaagaag gacttgttca tccagcttat
     5281 gactacgtcc ttaaatgttc acacaccttt aatttattag atgcacgtgg agcagtatct
     5341 gttactgagc gtgcaggcta tattgcacgt attcggaatt tagcacgtgt tgttgctaaa
     5401 acctttgttg ccgaacgtaa aaaacttggt ttcccacttt tggatgagga aactcgtatc
     5461 aaattacttg ctgaggaaga ctaatgaaca cactcattgt taatagtcat cctgattttt
     5521 cgaatccgta ctcatttaca actattctac aagaaaagtt tattgaactt tataatgagc
     5581 atttccctaa ccatcagtta tctattttaa atttatatga ttgtgttttg ccagaaatta
     5641 cgaaagaagt actactttcg atttggtcta aacaaagaaa gggtttggaa ttaacagccg
     5701 atgagattgt tcaagctaaa atctctaaag atttacttga acagtttaag tcacatcacc
     5761 gtattgtctt tgtttctcca atgcacaatt acaatgtgac tgctagagca aaaacttata
     5821 ttgataatat ttttattgca ggtgagactt tcaagtacac tgaaaatggt tcggtcggat
     5881 taatgacaga tgactatcgt ctgcttatgt tagaatctgc gggttctatt tattctaaag
     5941 gacaatatag tccatatgaa tttcctgttc attatttaaa agctatcttt aaagatttta
     6001 tggcttttga tgatttctgt gttgttcgtg cagaaggtac cgatatttta gataggcaag
     6061 ttgttcttga taagatgaat caggatttaa gagaagcatt tgaagcgttt tatagtaagg
     6121 agtaggaaat gactaaagat ttattattgg agcttggact tgaagagttg ccagcttatg
     6181 ttgtgacacc gagtgagaag caattgggcc aaaaaatggt caagttttta gaggatcatc
     6241 gtttatcatt tgaaactgtt caaacttttt caactccacg tcgtttggct gttcgtgtta
     6301 aaggattggc agaccagcag acagatttaa cagaagattt caagggtcct tctaagaaaa
     6361 ttgctttgga tgcagaaggg aatttttcta aagcagccca aggttttgtt cgcggaaaag
     6421 gtttaagtgt tgatgatatc gaatttcgtg aagttaaggg agaagaatat gtttatgtca
     6481 ctaagcatga aactggaaaa agtgcgattg atgttttagc gagtgtgaca gaggtattaa
     6541 cagaattgac tttcccagtg aacatgcatt gggcaaataa tagttttgaa tatattcgtc
     6601 cagttcacac tttagtggta ttattagatg atcaagcctt agagctagac ttccttgata
     6661 ttcattcagg gcgtattagt cgaggacacc gtttcttggg ttctgacact gaaatttcat
     6721 ctgccagttc gtatgaagat gacttgcgac aacaatttgt tattgctgat gcaaaagaac
     6781 gtcagcaaat gattgttaat caaattcatg ctatcgaaga aaagaaaaat atttcagttg
     6841 aaattgacga agatttacta aatgaagtgc ttaatttagt tgagtatccg acagcattct
     6901 tgggttcatt tgacgagaaa taccttgatg tacctgaaga agttttagtc acttcaatga
     6961 aaaatcatca acgctatttt gttgttcgcg acagggatgg aaaattgtta ccaaacttta
     7021 tttctgtgcg taatggaaat gctgagcata tcgaaaatgt tataaaaggc aatgaaaaag
     7081 ttttagtggc tcgtcttgaa gatggtgaat tcttctggca agaagatcag aaacttaata
     7141 ttgcagattt agttgagaaa ttaaaacaag taactttcca tgaaaaaatt ggttcacttt
     7201 atgaacatat ggatcgtgta aaagttattt cacaacattt ggctgataag gcaggtcttt
     7261 ctgatgaaga aaaattagct gtgcttcgag ctgcaagtat ttataaattt gacctcttaa
     7321 ctggaatggt tgatgagttt gatgaattac aaggtattat gggtgagaaa tatgccttac
     7381 ttgcagggga acagcccgca gtagcagcag cgatacgtga acattatatg ccgacatcgg
     7441 ccgatggaga gcttccagaa accagaattg gtgcaattct agcgttagct gataaatttg
     7501 acaccttact ttcattcttc tcagttggtt tgattccaag tggttcaaat gatccatatg
     7561 ctcttcgtcg agctactcag gggattgtcc gtattttgga agcttttggt tgggatatcc
     7621 cacttgatga attagtaact aacctttatg gtctatcgtt tgctagttta gattatgcta
     7681 atcaaaaaga ggtcatggcc tttatttcag ctcgtattga aaagatgatt ggctctaagg
     7741 tccctaaaga tattcgagaa gccgttttag aatcagacac ttatattgtt agccttattc
     7801 tagaagctag ccaagcattg gtgcaaaaga gcaaggatgc tcaatataaa gtttccgttg
     7861 aaagtttgtc acgtgcattt aatttagctg aaaaagttac tcattctgta tcggtggact
     7921 cttcattgtt tgaaaataat caggagaagg cgctatatca agctattttg tcacttgaac
     7981 tgactgagga tatgcatgac aacttagata agcttttcgc acttagtcca attattaatg
     8041 atttctttga taatacaatg gtaatgacag atgatgaaaa aatgaaacaa aatcgtctgg
     8101 ctttactcaa tagccttgtt gcaaaggcaa gaacagtcgc tgcttttaac ttattaaaca
     8161 caaaataagg aggcatttta tggatcctaa aaaaattgct cgtattaacg agctttcaaa
     8221 aaagaaaaaa acagttggat tgaccggaga agagaaggtt gaacaagcaa agctccgtga
     8281 agagtatatt gaaggtttcc gccgatcagt ccgtcaccac gttgaaggaa ttaaattagt
     8341 ggatgatgag ggcaatgatg ttaccccgga aaaacttcgt caagtccaga gagaaaaagg
     8401 actacatggt cgtagtttgg atgatccaaa ttcttaatgg tgagacaatt ttaaattaaa
     8461 gtcaacactt gggtggggca aaagataatc tttgtctcgc cttttttatt aggaaggaaa
     8521 aaataatgat tgaattacta ttagcattac tgttatggaa tattttagta tttgcaacct
     8581 atgctattga taaaaggcgt gcaattagag gggcatggcg tattagtgag aaaacattgt
     8641 tgatagagag tttattatta gggggatttg gtgcattcct aggtggagaa gtttttcgac
     8701 ataaaacgac taaatggtat tttagactag tttggtatat cggaatgatt attgatttag
     8761 ctattatttg gctaatttgg tgttattgat gatatcttat caaaaatttt aggggcatat
     8821 taaatttttg atagtgcagg atagactgat ttaggtataa tagaattaca aaaagaaaac
     8881 gcttactttt aaggaggaag tttatgtctt cagaagaaaa atacgttatg gcgattgacc
     8941 agggaacaac aagttctcga gcaattattt ttaataaaaa aggtgaaaaa attgcgagta
     9001 gccaaaaaga atttccccaa atttttcctc aagcaggctg ggtagagcat aatgctaatc
     9061 agatttggaa ttctgttcaa tctgtcattg ctggagcttt tattgaatca agcatcaaac
     9121 ctggacagat agaggctatt ggtattacca atcaacgtga aacaactgtt gtttgggata
     9181 aaaaaacagg tcttccaatt tataatgcta ttgtatggca atcgcgacaa acagccccga
     9241 ttgctgatca attgaagcaa gaaggtcata ctaatatgat tcatgaaaaa actggtcttg
     9301 tcattgatgc ttacttctcg gcaacaaaag ttcgttggat tttagaccat gttcctggag
     9361 cacaggaacg tgctgaaaaa ggagagttgc tttttggaac aattgatact tggttagttt
     9421 ggaagttaac tgacggtctc gtccatgtta cagactattc aaatgctgct cgtacgatgc
     9481 tttataatat taaagaactt aaatgggatg atgaaatttt agagttactg aatattccta
     9541 aagcaatgtt accagaagta aaatctaatt ctgaagttta tgggaaaact actccattcc
     9601 atttttatgg tggagaggtg ccaatttctg gtatggctgg tgaccaacag gcagcattat
     9661 ttggacagtt ggcctttgaa cctggtatgg taaaaaacac ttatggtaca ggttccttca
     9721 ttatcatgaa tacgggcgaa gagatgcaac tgtctcaaaa taatctatta acaacgattg
     9781 gttacggcat taatggtaag gttcattatg cacttgaagg atcaatcttc attgcaggaa
     9841 gcgctattca atggttacgt gatggtctta gaatgattga aacgtcaagt gagtcagaag
     9901 gccttgctca atcatcaaca agtgacgatg aggtttatgt ggttccagct tttacaggtt
     9961 tgggtgcacc gtattgggat tcaaatgcta gagggtcagt atttggatta actcgtggga
    10021 caagtaaaga agattttgtt aaagctacac ttcagtcaat tgcttatcaa gtacgagacg
    10081 ttattgacac tatgcaagtt gattcaggaa ttgatatcca acaacttcgt gtagatggtg
    10141 gagctgcaat gaataattta ttaatgcaat tccaagcgga tattttaggg attgatattg
    10201 ctcgtgcgaa gaaccttgaa actactgcct tgggtgcagc tttcttagcc ggactttcag
    10261 ttggctattg ggaaagtatg gatgaactaa aagaactgaa cgcaacagga caacttttcc
    10321 aagcaacaat gaatgagtct cgaaaagaaa aactgtataa agggtggaga aaagctgtca
    10381 aagcgacaca agtatttgct caagaggatt aagggggtgt agagatggaa ttttcacgag
    10441 aaacacgacg tttagcctta caaagaatgc aagatcgtac tctggattta cttatcatag
    10501 gtgggggtat cacaggcgct ggtgtcgctt tacaagcagc agctagtgga ttagatactg
    10561 gacttattga aatgcaagat tttgcagaag ggacaagtag tcgctcaaca aaattggtcc
    10621 atggtggtct tcgttacttg aagcaatttg atgtagaagt tgtttctgat acagtatcag
    10681 agcgtgcagt cgtgcagcaa attgcaccac acattcctaa gccagatcca atgcttttac
    10741 cggtttacga tgagccgggt tctactttta gtatgtttcg ccttaaagtg gctatggatt
    10801 tatatgattt attagcaggt gtgacaaaca cacccgcagc caataaagtt ttaagcgcag
    10861 aagatgttct taagcgtgag cctgatttac aaaaagaagg tttgctaggc ggaggggtct
    10921 atttagattt ccgtaataac gatgcacgcc tagtgattga aaatattaaa cgtgctaata
    10981 gggatggagc ttatattgcc agtcatgtca aagctgagga cttcttattt gatgacaaca
    11041 atcaaattat aggagtacgt gcgcgtgatt tgttgactga ccaagttatc gatataaaag
    11101 ctagacttgt tattaataca actggacctt ggagtgacac cgttcgcaac ttctccaatg
    11161 aaggtaaaca aattcatcaa ttacgtccaa ctaagggagt tcacttagtt gtagatcgcc
    11221 aaaaattaaa tgtttctcaa cctgtttatg ttgatacagg tctaaatgat ggtcgcatga
    11281 tttttgtttt gccacgtgaa gataagactt attttggaac cacagatact gattaccatg
    11341 gagacttaga acatcctacg gtaactaaag aagatgttga ctacctcctt aatattgtaa
    11401 acaaacgctt cccagaagca gagcttacta ttgatgatat tgagagttca tgggcaggtc
    11461 tgcgtccact actatcaggg aatagtgcct ctgactataa tggaggcaat agtggaaaac
    11521 taagcgatga aagttttgaa gaattaattg attccgttaa agattatatt gcacataaaa
    11581 atcatcgtga ggatgttgaa aaagctattt ctcatgtaga atctagtact tctgaaaaag
    11641 aattggaccc atcagctgtt tctcgaggat caagttttga gcgtgatgat aatggcctat
    11701 taacacttgc tggtggtaaa attactgatt atcgaaaaat ggcagaaggt gccatggaaa
    11761 ctattattaa cattttggat aaagaatata atcgcaagtt taaactaatc aattctaaaa
    11821 cctatcctgt ttcaggagga gagattaacc catctaatgt tgattctgaa attgaagctt
    11881 atgctcaatt gggcacacta agtggtttaa gtattgagga tgcacgttat attgctaacc
    11941 tttatgggtc aaatgcacca aaattatttg cattgacacg ccaaattaca gaagcagaag
    12001 gtctgagctt ggtagaaacg ttgtcacttc actatgctat ggattatgaa atggcgctta
    12061 gcccaacgga tttcttctta agacgtacga atcatatgct tttcatgaga gataatttag
    12121 attctttgat tcagcctgtt attgatgaga tggctaaaca ttatcaatgg tctgatcaag
    12181 acaaaacttt ctatgaagaa gaattacatg agactctaaa agacaatgac ttggcagctt
    12241 tgaaggataa ttgaaggaga taataatgga tatttttgga gaattcttag gaacagctct
    12301 cttagtctta cttggtaatg gtgttgtagc aggtgttgtt ttgcctaaaa cgaagaatca
    12361 taattctgga tggattgtta tcacatttgg atgggggctt gctgtagcta ttgcagcgtt
    12421 ggtatctggc aatattagcc ctgcccactt gaatccagct gtgtcattag cttttgcgat
    12481 caaaggtgat ttagcttggg gaacagctat tctttatatg attgcacaga ttattggtgc
    12541 aatgctaggt tcacttcttg tttatttgca atttagaccc cattatgagg ctgcagaaaa
    12601 taaagcagat atccttggaa cttttgcaac tggtccggct ttgaaggata atttttccaa
    12661 ttttttaagt gaagtattgg gaacacttgt tcttgtactt actatttttg ccatcggaaa
    12721 atacaatatg cctccaggag ttggtacgat gagtgttggt atgttagtgg ttgggattgg
    12781 tctatcgctc ggtggaacaa caggttatgc tattaatcct gcacgtgatt ttggtcctcg
    12841 tttattgcat gccttgttac cgatgaagaa caaaggtgat tctgattgga cttattcatg
    12901 gattccgata gtaggaccta tggtaggagc tattttagca gccttaatat ttgctatgat
    12961 gtaagagatt tttgatgctg agaccgactt tatggtctca gttttttgtt accgttttca
    13021 aaaataactt cttttgttat aataagtaat tatgacagaa aaatatgtaa ataaagtaat
    13081 tgtcatttta ggagcttctt ttgctggtat gacttgtgcg cagaaattac gccaattaaa
    13141 tcctaattgg gatatcgttt tgattgataa agaaattcat ccagattatg ttccaaatgg
    13201 cttaaattgg tattatcgtc acgaaattag tggtttaaat caggccatgt ggcagacaga
    13261 agaagagcaa cggttgcaga atatacgctg tctatttggt ctaaaagttg agaaaattaa
    13321 taaagaagat agggaattaa tgttatcaga tggttcttct gtctattatg atcaattgat
    13381 ttgtgccatg ggctcccaag cagaatcaac ttatattgat ggggctgatg cgcaaggtgt
    13441 tttaacaaca aaaacttatg caactagtca aaatgctaag caggtattag ataaaagtca
    13501 taaggtagca gttgttgggg caggtatcat cggtttagat attgcttata gtttacatga
    13561 atcagggaag gcggtcactt tgttagaagc tcaagaaaga ccagacttca ggcatacaga
    13621 tcctgatatg tcccttcctt tacttgatgc gatggctgaa tcaaaactac attttttcca
    13681 aaatcaaaag gttgagaaaa ttactgtaac ccgagaagaa aaactttgtt tgcgaacgct
    13741 aacaggagac acctttacag ttgatactgt catattagct gttaattttc gtccggatag
    13801 tcgtctttta actgggttgg ttgatttaag tgttgataat agtgttgttg taaatgatta
    13861 tttccaaacg agtgacccta atatctatgc tattggcgat ttgatttggt cgtattttaa
    13921 aggtcttaat agtgcttatt atatgccctt aattaaccaa gcaatccgtt cggcacaaat
    13981 gttggcatac catttatcgg gacatgctgt tccaaaatta aaaataacac gcgctacagg
    14041 atctaaacac tttggctatt atagagctaa cattggatta actgagctgg aagctggtct
    14101 ttacgaggat accgttagtg ttacttattt ccctaaagaa cagtatgatt taaggataaa
    14161 attgattgct aatcaaaaaa cgggccattt attgggggct caattaatct caaaggaaaa
    14221 ttgtttggct actgccaatc agcttgttca agctatatcg tgtgacatga cagattttga
    14281 tttggctttt caagatttca tttatacagc tagagaatct gagatggctt atatgttaca
    14341 tcaagcagct atcaaccttt atgaaaagag gattgggcta tgcgaataga agctctaatg
    14401 gaaaaagaaa ggcgtgttca atatcggcta ctttcttttt taagaggctc tcctcaggca
    14461 atagcactga agttagcttt attagaaact ggtttaagtc gagcaacttt gcttaaatat
    14521 atcaataatc ttaatagcta ctttgaacaa gaaaaagtta attgtcgaat cgtttattat
    14581 aaggataagt tatttttaga agaggattat aacttatcca atcaagaagt gttgaaagct
    14641 ctgatgaaag atagtattaa atacactatc ttaatatctt tatttaatca gcgtcagttc
    14701 acaatcgttg gtctttctca agaattgatg gttagtgagg ctactttaaa tcgtcatctt
    14761 gctcatctca atgaattgtt agcggaattt gatattgcta tttcacaagg aaaacagatt
    14821 ggtgatgaat tgcaatggcg ttatttttat tatgaattat tcaagcaact atggtcttat
    14881 gacaagtgcc aaaatatgat aaagaaatta gacttagact cattaattct attaatagag
    14941 cgcttggctc aacatacttt aactcgagaa gctcagcaaa atctggggct ttggtttagt
    15001 atttgtcatc atcgtctcct tgctatggaa aaactatctg ataatctcaa gcccattgtg
    15061 gaacattatc aatgtaatgc tttttataaa cggttagacg cagccttagt tttgtatatg
    15121 agtcgttttg ctcttgagta tagggaagga gaagttctgg caacttttgc ttttctacat
    15181 tcacaaaata ttttaccaat caatacaatg gaatacatca tgggatttgg aggcccgatt
    15241 attgattgtg taaccgaaac aatcatctac tttaaaaagg agagtatttt agctgacgag
    15301 acatcagatc aggttattta ccaacttggt cagttatata gccattatta tttctttaaa
    15361 ggtcatattt tagttgaaca acctgattta gaacaaacgt atcgacttat tgatcataat
    15421 atgagggata agttgcatga tatttcaaag aagattattg caaatgttaa tcgaattaga
    15481 cctttaacag aagatggttg ttctttgctg actttacatt tgttggaatt gcttattttc
    15541 tcgaaaaata gtcaaaaaat gccctttagg attggcctag atatgacagg taatgctgtg
    15601 gaacagtcat tgttggaata tcgcattcga caacatttct ctggtaataa ttctattcaa
    15661 gtcgaacctt atgacgaagg aaagggattt gatatggtta tttatcagag tcgctcaaga
    15721 ccttataaag ccaaactaac ctattgcctt aataaaggtg cttcagagcg agaactacag
    15781 gaaatagatt cacttattta tgacaattaa tcccattact taattgaatg gttttaattc
    15841 ctccagaaat tgttaaaacg tttacgtatt ttatgttata atatcaaaga cgaacaattt
    15901 taaatttagt tgttaaaaac agaaaggttt tatcatgaca tttgacacca ttgatcaatt
    15961 agcggttaat acagtccgca cgctttctat tgatgctatc caagcagcaa attctgggca
    16021 cccaggtctt cctatgggag ctgcgcctat ggcttatgtg ctttggaata aattcttaaa
    16081 tgtaaaccca aaaacaagtc gcaattggac aaaccgtgac cgttttgtac tttcagctgg
    16141 gcatggttca gctcttcttt atagcctact tcatttagct ggctatgatt tatcaattga
    16201 tgatttaaaa caattccgcc aatggggatc aaaaacacct ggacatccag aagttaacca
    16261 tactgatggt gtagaagcaa caactggacc tttagggcaa ggtattgcta atgctgttgg
    16321 tatggcaatg gctgaagctc atttggctgc taaatttaat aaaccaggct ttgatttagt
    16381 tgatcattac acttatacac ttcatggtga tggttgtttg atggagggtg ttagccaaga
    16441 agctgccagt cttgctggac acttaaaact tggtaaattg gtccttcttt atgattcaaa
    16501 tgatatctca cttgatggtc caacgtctca atcatttaca gaagatgtta aagggcgttt
    16561 tgaatcatat ggttggcaac atatcttagt taaagatggt aatgatttag aagcgattgc
    16621 tgcagcaatt gaagctgcta aagctgaaac tgataaacca accattattg aagttaaaac
    16681 tattattgga tttggcgcag agaaacaggg aacatcttca gttcatggag cgccacttgg
    16741 tgcagaaggt attacttttg ccaagaaggc ttatggatgg gaatatccag actttactgt
    16801 tcctgctgaa gttgcagctc gatttgcctc agatcttcaa gcgcgtggtg cgaaggcaga
    16861 agaggcatgg aatgatcttt ttgctaaata tgaagttgag tatccagaat tagcagctga
    16921 atacaaagaa gcttttgctg gccaagctga gacagttgaa ttgaaagctc atgacctagg
    16981 ttcatcagtg gctagtcgtg tttcaagcca acaagctatt caacaattat cgactcaatt
    17041 accgaacctc tggggagggt cagcagacct ctcagcttct aataacacaa tggtagcagc
    17101 agaaacagat ttccaagcaa gtaactacgc tgggcgtaac atttggtttg gtgtacgtga
    17161 atttgcaatg gctgctgcaa tgaatggaat tgcgcttcat ggtgggactc gtgtttatgg
    17221 gggtacattc tttgtcttct caaactactt gttaccagca gtacgtatgg ctgcacttca
    17281 aaacttgcca actgtctatg ttatgacaca tgattctatt gctgttggag aagatgggcc
    17341 aactcatgag ccaatagaac aattagcatc agtgcgctca atgcctaatt tgaacgttat
    17401 ccgtcccgca gacggtaatg aaacaaacgc tgcatggcaa cgagctgtat cagagacaga
    17461 tcgaccaacg atgcttgttt taacacgtca aaacttacca gttcttgaag gaacatcaga
    17521 attagcccaa gaaggggtta ataaaggtgc ttatatttta tcagaagcta aaggtgaact
    17581 tgatggtatc atcattgcaa caggttcaga agttaaatta gctttagata ctcaagacaa
    17641 acttgagtca gagggcatcc atgttcgcgt cgtatctatg ccagcacaaa acatttttga
    17701 tgaacaagag gcttcatatc aagagcaagt gcttccatca gctgttacaa aacgtcttgc
    17761 tattgaggca ggatcaagct tcggttgggg taaatatgtt ggtttaaatg ggctaacact
    17821 tactattgat acttggggag catcagcacc aggtaacaga attttcgaag agtatggctt
    17881 tacagttgag aatgctgtat cgctatataa agaactttaa gagcatgaag aagttgggaa
    17941 aaatacctca acttcttttt gtaagatata ggatggattt gagaatagtt ttgatagact
    18001 gataagtgat ggaaagcact cttttttgaa aacgtttgac aaacattcca tagcgcagta
    18061 taattgataa aactattgta tatataaaat aatacaacta ttattaatta tctttttaga
    18121 aaggtttgtt tatgaagaag gagtgtcgtg atttttatcg gcagatacag catacttaca
    18181 atgatatatc tgtccgtgaa gatgcagtgc tatcatcaat actattatca gcatcgaatg
    18241 gtttgataaa aactagcgat gtacctcgtg ttgcttatga gttaactcag caattggaaa
    18301 ataatgagat agaaaaatct ttcgagtcat tagctacagt taaggaatta aaaaaatctg
    18361 ctaaaaaatt gtctgagaga tatgagcaaa ataaggtaac aacgggattg ctttctaaag
    18421 tatttggaaa tcattaggag ataaaagaag atttttatga ttaagtttga acatgtttcg
    18481 aaagtatacg gggaaaaaga agctctcagt gatttaaccc tctccattaa agatggtgag
    18541 atttttggat taatcggtca caatggagca ggaaaaacaa cgacaattag tattctaaca
    18601 tctattattg atgcaactta tggccaagtc tatattgatg acctactttt gacagaacat
    18661 cgtgaccaaa ttaagaaaaa aataggttat gtaccggact cgccagatat tttcttgaat
    18721 ttaacagcag aggaatactg gtacttttta gctaaaattt atgatgttgc cccagaagat
    18781 attgaagcac gtatcacaaa attggtagac atatttgaac tagaggagca gcgctataat
    18841 cctattgaaa gtttttcaca tggtatgcgt caaaaagtca ttgttatagg tgcccttctt
    18901 ccaaatcctg acatttggat tctagatgag ccattaactg gtttagatcc tcaagcttct
    18961 tttgatttga aagaaatgat gaaagaacat gctaaaaatg gaaaaacagt tattttttca
    19021 acacatgtct tagcagttgc agaacagtta tgcgacagaa taggtatttt gaaacaaggg
    19081 aaattaatct ttgtcggtag cttaggtgag ttaaaaatgc aatatccaga taaagatttg
    19141 gaaactatct atttggaatt agcaggacgt caagctaata gggagggata aagtatgaat
    19201 tggtcacgta tctgggaact cgtaaaaatt aatatccttt attcaaaccc tcagactcta
    19261 tcggcactaa gaaaaaagca agaaaagcat cctaaaaaag aattttcagc ttataaatcc
    19321 atgtttagaa atcagttatt tcagattttg ctcttttcaa taatttatgt atttctcttt
    19381 gtatcacttg attttaaaga atatccgggc tatttcacgt tctacattgg tatctttaca
    19441 ctagtatcca ttatctactc ttttattgcg atgtacagtg ttttctatga gagtgacgat
    19501 gttaaacaat atgcttattt gcctattaaa tctgaagagc tttatgttgc taaaattttt
    19561 gcaacatttg ggatgtctgt aacttttcta atgccaatcc taaccttaat gattgtggct
    19621 tattggcgta taataggtgg cccacttgcg gtcctcctag caatcataaa ttttgctatt
    19681 ttatttttat cggtaacggt gatttcattg tatattaata gtttaatagg ccgtgccatt
    19741 attcgaagtg ctaatcggaa acttatttct actatattaa tttcactagc tacatttgga
    19801 gcgatagttc ccttattatt tgtgaatatg accagtcaaa agatggtaca agggaaatta
    19861 caagatatcg ctccaatttc ttatgtcaga ggttattatg atattgttac agcgccgttt
    19921 agtatggaga gtcttctcaa ctattatctc ccgctcttaa tcattttatt cttgataggt
    19981 gctatttata aatgggttat gccacgctac tatcaggagt tgttgtatgg acaggtcaag
    20041 cagagaaaag tccatcgtca aatcgatttt agtaaacgtg agtcaattaa taaaactctt
    20101 gttaaacacc atttatcaag tttacaaaat gcgacattat tgacaaatac atttttaatg
    20161 ccactattat atctagcaat gttcattgtt ccaattttga ataatggtaa agagattggt
    20221 cgatttttta atgaaaatta ttttgggata gcttttctag caggaatatt aataggtagt
    20281 ttatgtgtga tgccagctag cattgtaggg gtaggtattt ctttagaaaa aagtaatttc
    20341 tactttataa aaagtttgcc tatatcattt tcgtatttct taaagcataa atttgtaact
    20401 ttgataacat tacaacttgc agttcctact tttatttatt tcttggtagg attctttcta
    20461 cttaaactat caatcctcgt tttattgagc ttcattttag gtctagtctt catgggactt
    20521 atcgaaggcc agtttattta tagacgtgac tataaacatt tgttcttgaa ttggcaagag
    20581 gttacgcaac tatttaatag agggttaggc caatggctgt tagttggttc attgtttgga
    20641 atgatgataa taggtagttt tcttattgga ataagtattt tttggtcaat ggtatggaat
    20701 actgtagctg tcaatatcat aatccttatc attgggttgc tcatattatc aatttgccaa
    20761 tacttactgc taaaaaattt ttggaagaag cttgtttagt aaaaagattt tagaaaaatc
    20821 atacagtaag aataatgctt aataactgag atagttatag cggataaaat aaaggcttgt
    20881 tgtggacttt acggttggaa ggcagccact gtaatccttt gtttaataac gctagtttta
    20941 gctctaaaat atttgatttt tattgctatc tattatatct tatcagacaa agagactagg
    21001 gataacctag tctctttgtc tgatgctaac ttgtttttcg taatattgtg ctagaatgaa
    21061 tgtcaatata gcagttgtgc ttacagtaat gataactgca ataagtatgg taataaaaag
    21121 atgtgttgaa gtatgactga tataaagaaa tagacctggt agaccagaag agcctacagc
    21181 gaagcggtga gcgttcatta taccggcata gagtcctcca agaccaccac caataagtga
    21241 ggctattaga ggataacgtt tcggaaggtt gacaccatat aagataggtt cggtgattcc
    21301 catataagct gttatggcag caggtccagc aatttcttta agcttttttt cttttgttat
    21361 aagagtcacc actgtacctg cagttgcttg tgcgatattt gagcagagca tgcctggtcc
    21421 aaaaatagaa tcaaccccta atttcgctag ttgtgtgatt cctaatgcag caattccact
    21481 atgaacacca aacatgatta ggataggagc aaatgctcct actagaaaag caggtgccca
    21541 actggcgtat ttggataaaa aaagtaaaaa taacagctaa atattggcca gcgatagtac
    21601 ccattggtcc caatatagag aacgacaaaa ttcccatgct gagaaatatt aacataggaa
    21661 caagcactaa acgtagtgat ttaggaataa tttgatttaa atatttttct atataagcct
    21721 gaacacaaat aattagaata atgggaatga cacttgatgc atagttagta agtgtgaatg
    21781 ggatcgtatg gaataaggat actggtttac cagaagctac taaattagcc caattagggt
    21841 gaaggagcat gacaacagtt cccaaagcta aaataggatt agccttcaat ttttgagcag
    21901 ctgtgatggc gattaagata ggtaaaaaat agaaaacacc atctgcaaaa agatttagta
    21961 atatatatgt ttgagataac ggtgtcaaga ttttgaatac tagtaataaa gctagcaatg
    22021 ccttaatcat accagcgcct gatagagctg gtaaaatcgg ttggaaagtc ccagaaacaa
    22081 aatcaataat tcgactaact gctttggctt ttttggtttg ttctgtttta gtcgttgagt
    22141 tagtttcaat gagggagtta atttcttcaa acacttttgc gacatgagtc ccgatcacta
    22201 tctgatgttg ccctccgctg ataacgacag tttttacctc ttttaaagtt tttaattgct
    22261 caaggtttac tttcgtcgga tcattgagac taaagcgaag gcgtgtttga caatggtaaa
    22321 cattgttaat attattttgc ccaccaaggt gtttgatgat attttttgac aaggtgatgt
    22381 attctctaga cataatatct ccttaatact tataaaacgt tttccgattc tagattatag
    22441 catagtaata aaagaaaaca cttggaaatg cttgtgaatt ttcaatgatg ttttcagata
    22501 gcattcctaa agggtctatg atataatttt tttatgaaaa gacattttga gacaactaga
    22561 agaattgtta ttaaagtagg aaccagttcc ctcgtacaga caagtgggaa aattaattta
    22621 agtaaaatag accatttagc atttgttatt tctagtctta tgaataaagg tatggaggtg
    22681 attttagtat catctggagc tatggggttt ggtttagaca ttttgaaaat ggataagcgt
    22741 cctcaagaaa tatcacaaca acaagcagtt tctagcgtag gacaagtagc catgatgagt
    22801 ctttattctc agatattttc gcactatcaa actcatgttt cccaaatttt attaacacgt
    22861 gacgttgtgg tttttccaga gagtttgcaa aatgtaacaa actcttttga gagtctgctc
    22921 tctatgggaa ttttgccaat cgtcaatgag aatgatgctg tcagcgtgga tgagatggat
    22981 cataaaacga agtttgggga caatgaccgt ctctctgctg tcgttgctaa aattactaaa
    23041 gcagatttat taattatgct gtcagatatt gatggtcttt ttgataaaaa tcctaatatt
    23101 tatgatgatg ctgttttacg tagtcatgtt tctgagatca ccgatgacat cattaagtca
    23161 gctggaggag caggaagtaa gtttggtact ggtggcatgc ttagtaaaat taaaagtgca
    23221 caaatggtgt ttgataacaa tggacaaatg atattgatga atggcgccaa tcctcgtgat
    23281 attttaaagg tgttagatgg gcataatatt ggaacttatt ttgctcaagg aaagtgagat
    23341 tgagttatga cgtatattga aatattaggg caaaatgcta aaaaagctag tcaatcagta
    23401 gcacgattaa gtacagctag taaaaatgag atactcaggg acttagcgag aaacatagtg
    23461 gctgatactg aaaccatttt gactgagaat gctcgcgatg ttgctaaggc aaaagataat
    23521 ggtatctcag aaattatggt ggaccgtcta cgactaaaca aagaccgcat acaggcgata
    23581 gcaaacggta tttatcaggt agcagatttg gcagatccga ttggtcaagt tgttagtggt
    23641 tatacaaatt tagatggttt aaaaatcctt aaaaaaagag tgcctttagg ggttattgca
    23701 atgatatttg aaagtcgtcc caatgtatcg gtggatgcct ttagtttagc ttttaaaaca
    23761 gggaacgcta ttattttacg tggtggtaaa gatgctattt tttcaaatac tgccttggtt
    23821 aattgtatgc gccaaacatt gcaagataca ggccacaacc cagatatagt acaattagtt
    23881 gaagatacta gtcatgttgt agcagaggag ttaatgcagg caacagacta cgtggacgtc
    23941 ttaattccaa gaggtggtac aaaattaatt caaacggtga aggaaaagtc aaagattcct
    24001 gttatcgaaa caggtgttgg aaatgttcat atctatattg atgaatttgc agatttagat
    24061 atggcagcta agattgttat taatgctaaa acccaacgcc ctagtgtctg taatgctgct
    24121 gaagggttag ttgttcatca agctattgct aagggatttt tatctcaatt ggagaaaatg
    24181 ctcaaagaat cgaaccagtc agttgaattt cgtgccgatg aggaagcttt acaactttta
    24241 gaaaatgctg ttgctgcaag tgagactgat tatgctactg aatttttaga ctatattatg
    24301 tcagtaaaag tagtagattc ttttgaacaa gcaattagct ggattaataa atacagtagt
    24361 catcactcag aagctatcat tactaataat atcagtaggg cagaaatttt tcaggatatg
    24421 gtagatgcag cggcagttta tgttaatgct tcaacacgtt ttacagatgg tttcgttttt
    24481 ggactcggag cagagattgg tatttcgact caaaaattac acgcgcgtgg accgatggga
    24541 ttagaagctt taacaagtac aaaatactat attaatggaa ctggtcaagt aagggaatag
    24601 ttgacctata ataggagaga ttgcatttat cggtctctct tttacttttc taaagtttag
    24661 gatttatggt acaattaaag ttatgacaaa cgattttcat catattactg tgcttttgca
    24721 cgagactgtg gatatgcttg atattaagcc ggatggtacc tatgtagatg ctactttagg
    24781 tggggcagga cacagcgaat atcttctttc acaattaggc ccagatgggc acctttacgc
    24841 atttgaccaa gaccagaagg ctattgacat tgctcatata cgtttaaaga aatatgtgga
    24901 taccggtcaa gtaaccttta tcaaagataa tttcagaaat ctatcttcta acttaaaagc
    24961 attaggagtg tcggaaatta atggtatttg ttatgattta ggtgtatcaa gcccacagct
    25021 ggacgagcgt gaacgcggtt tctcatataa acaagatgcc ccccttgata tgcgaatgaa
    25081 tagagagcag agccttacag cttatgacgt tgtgaacact tatagttatc atgatttagt
    25141 aaggattttc tttaagtatg gcgaagataa attttcaaaa caaattgctc gaaaaattga
    25201 gcaagtacga gctgaaaaac caatatctac aacaacagaa ttggctgaga ttatcaaatc
    25261 gtctaaatct gccaaagaac tgaagaaaaa aggtcaccca gctaaacaaa tttttcaagc
    25321 tattcgtatt gaagtcaatg acgaactagg agctgcagat gaatctatac aacaagcaat
    25381 ggacttatta gctgttgatg gtcgtatatc tgtcatcact tttcattctt tagaagatcg
    25441 tcttactaag caacttttta aagaagcatc aacagtagaa gtacctaaag gtcttccttt
    25501 tataccagat gatttacaac ctaagatgga gctcgttaat cgtaaaccca tcttaccaag
    25561 tcaagaagaa ctagaagcga acaatcgtgc ccattcagcg aaattgagag tagcgcgtcg
    25621 tattcgataa taagaaagaa acttatgaca aatgaaaaac gaactgaagc agtaacacaa
    25681 accttacaac gtcacatcaa gactttctct cgcattgaga aagcttttta tggcgctatt
    25741 gtaattactg ctattatcat ggcagtcggt attatttact tgcaaagtaa tagtttacaa
    25801 gttaaacaag aagtaaatca attaaatagt aaaatcaacg ataaacagac agaatttgac
    25861 aatgctaaac aagaagtaaa tgagctttct aatcgtgatc gaattactaa aattgctaaa
    25921 gatgctggtt taacaattca aaacgacaat atttatcgga aggtggacta gtctgtgact
    25981 ttttttaaaa agctaaaaaa aatcttcctt gattatgtga tccatataag agataggcgt
    26041 tcgccgcaga aaaataggga aagagtaggt caaaatctga tgattttaac cattttccta
    26101 tttttcattt ttatcatcaa ctttgtcatc atcgttggta cagatagtaa atttggggtt
    26161 aatttatcca aggaagccaa gaaagtctat caacaaagta tgacagtaca ggcgaagcgt
    26221 gggacaattt acgaccgtaa tggaaatcct atcgctgagg atgctacgac ctatagtctc
    26281 tacgctatta tctcaaagaa ctatacgaca gctacaggtc aaaaactata tgtacaacct
    26341 tctcagtatg aaaaagtagc tagtatctta gagaataaat tgggcatgaa gaaaaatctt
    26401 gtcctaaaac aactcaatca aaagaaattg tttcaagttt cctttggaag ttcaggttca
    26461 ggattgtctt ataccaagat ggctgacatc aagaaaacga tggaaaagtc agatatcaaa
    26521 ggaattggtt tttctaccag tccggggcgt atttatccaa atgggatatt tgcttcacaa
    26581 tttataggat ttactcttcc tcaggatgat ggagatggaa aaaaattagt tggtaataca
    26641 ggccttgaag cggcacttaa caaggtatta tcaggtactg atggcaaagt aacatacgaa
    26701 aaagaccgta gtgggaatgt tcttttaggt actgcaacaa cagaacgtag ggcggttaat
    26761 ggtaaggata tctacacaac tttatcagag cctattcaga ctgttttaga gactcagatg
    26821 gatgttttcg cagaaaaaac caaaggaaaa tttgcgagtg ctacagttgt caacgctaaa
    26881 acgggagaaa ttctagcaac gagtcagcgt cctacttata atccttctac ccttaaaggt
    26941 tacgataaga aaaatttggg tacctataac acactactat atgataattt ttttgaacct
    27001 ggttcaacaa tgaaggtaat gactctagcc tctgcaattg attctaagca ttttaactct
    27061 actgaagtgt ataacagtgc acaatataaa attgcagatg cggttattcg agattgggat
    27121 gtcaatgaag ggttatcatc tggtagttat atgacttttc cacaagggtt tgcacattcc
    27181 agtaacgttg gtatggtaac cttggaacaa aaaatgggac gagataaatg gttaaactat
    27241 ctttctaagt ttaaatttgg ttacccaaca cgttttggga tgcttcatga aagtggaggt
    27301 ttgttcccat ctgacaatga agtaactatc gctatgagct catttggtca aggtattggt
    27361 gtaacgcaag ttcaaatgtt gcgcgcattt acgtctatct cgaatgatgg tgttatgtta
    27421 caaccacagt ttattagcag tatttatgat ccaaatacag ggacttctcg tacagcacga
    27481 aaagaagtcg ttggaaaacc agtttccaaa gaagcagcta gtaaaacacg tgattatatg
    27541 gttactgttg ggacagatcc ttattatggt acattatacg cagcaggtgc cccagttatt
    27601 caggtgggga atcaatcagt tgcagtaaaa tctggtactg cccaaatcgc ccaggaaggt
    27661 ggtggtggtt atctgcaagg aaaaaatgat actattaact cagttgtggc tatggtgcca
    27721 tcggaaaatc ctgacttcat catgtatgtt accatccaac aacccgaaaa atttagtatc
    27781 acattctgga aagatgttgt taatcctgtt ttagaacaag caacagcaat gaaagaaacg
    27841 atattaaaac cggtattgaa tgactcagaa catcagacaa aatataaact atcaaaaata
    27901 gttggtgaaa atccaggtca tgtagcggaa gaattacgtc gtaatctggt tcagccaatt
    27961 attttaggaa atggtagtaa agtttctaaa gtatctaaaa gaccaggagc taatcttgct
    28021 gagaatgaac agctactagt attaacaaat aaactgactg aattacctga tatgtatggt
    28081 tggtctaaag caaacgttga acagtttgct aaatggacag gtataaaagt gacctataaa
    28141 ggttcaacct caggtaaagt aagaaaacaa agtattgatg ttggaaaatc catcaataaa
    28201 attaaaaaaa tcaaaattac aataggagat taacatgttt ttaagtataa tggcaggtgt
    28261 catagcattt gtcctgacag ttattgccat tccacgcttc attaagtttt accaattgaa
    28321 gaaaattggc gggcaacaaa tgcatgaaga tgtcaaacaa catctagcca aagcaggtac
    28381 gccgacaatg ggaggaacgg tatttttggt tgttgctctt cttgtctcac ttatttttag
    28441 tattatttta tctaaagaaa atagtggtaa tttaggtgca acttttggta ttttatctgt
    28501 tgttctcatc tatggaatta ttggtttttt agatgatttt ttgaaaattt ttaaacaaat
    28561 caatgagggc ttgactccaa agcaaaaaat gtcgctccaa ctaattgcgg gtctaatttt
    28621 ttattttgtc catgtgttac caagtggcac atctgcgata aatatttttg gttttaacct
    28681 tgaagtagga tacttatacg ctttttttgt tcttttctgg gttgttggtt tttcaaatgc
    28741 tgtgaaccta acagatggaa ttgatggtct agcttctatt tcggttgtta tcagtttgat
    28801 tacttatgga atcattgctt ataatcaaac gcaatttgat attttattga ttatcgttat
    28861 catgattggt gctttacttg gcttttttgt ctttaatcat aaaccagcta aagtctttat
    28921 gggagatgtt ggttcacttg cattgggtgc tatgttagca gccatttcaa ttgccttacg
    28981 tcaggaatgg accttgcttt ttatcggctt tgtctatgtt tttgagacgt cttcagtgat
    29041 gttgcaagta gcatacttta agtatactaa gaagaaaact ggtgttggga aacgtatctt
    29101 ccgcatgaca ccatttcatc accatttaga attaggtggt gtgtctggca aaggtaataa
    29161 atggagcgaa tggaaagtag atgctttttt atgggctatt ggtattttca tgagcgctat
    29221 tacacttgct attttatatc tttaaacttt attaggggat gtggtatgga ctgccagatt
    29281 cccttttgct ttcttgtagc ctttaatggt ataatgaaca taataatagt aaggaaatag
    29341 acatgtcatt taaagatttt aattttaaac catacattca gagggctctc gatgagttaa
    29401 aattcgtcga tccaactgat gttcaagcta aattgattcc agttgttcgt tcaggccgcg
    29461 atcttgtggg ggaatcgaaa actggttctg gtaaaaccca caccttttta cttcctattt
    29521 ttgagaagct agatgaaagt agtgatgatg ttcaagttgt tattactgca ccaagtagag
    29581 agttggcaac acaaatttat caggcaacta aacaaattgc tgagcattca gagcaagaaa
    29641 tacgggtggt taactatgtt ggtggtactg acaaattacg tcaaattgaa aagttgaaag
    29701 taagccaacc tcatattgtc attggaaccc caggacgtat ttatgatttg gtgaagtcag
    29761 gtgatttagt tattcataag gcgcatactt ttgtggttga tgaagcagat atgactctgg
    29821 atatgggatt ccttgatacc gttgataaga ttgcagggtc tttacccaag gatgttcaaa
    29881 ttttagtctt ttcagcaacg attccacaaa aattacagcc atttttaaag aaatatttga
    29941 ccaatccagt aatggaaaaa atcaaaacag caacagttat cgctgatacc atcgataact
    30001 ggcttttgtc cactaaagga cgtgataaaa atgcacaaat tttggaactc tctaaactaa
    30061 tgcagcctta ccttgcaatg atatttgtta atacgaaaga gcgtgcagac gaattacact
    30121 cgtatctttc tagtaatggc ttaaaagtag ctaaaattca tggtgggatt gcacctcgtg
    30181 aacgtaaacg tattatgaat caggttaaaa acctcgaatt tgaatatatc gttgcaacag
    30241 atttagcagc gcgtggtatt gatattgaag gtgttagcca tgttatcaat gatgccatcc
    30301 ctcaagacct atctttcttt gtccaccgtg tcggtcgcac aggccgtaat ggcttatctg
    30361 gtactgctat aactttatat cagccaagtg acgattctga tattcgtgaa cttgaaaaac
    30421 taggtattaa ttttattcct aaagtcatta aaaacggtga atttcaagat acatacgacc
    30481 gtgatcgtcg taataaccgt gaaaagtcct atcaaaaatt agatacagaa atgattggac
    30541 ttgtgaaaaa gaaaaagaaa aaaataaaac caggttacaa gaaaaaaatt caatggaaag
    30601 ttgaagaaaa gcgtcgcaaa gagcgtagag caagtaatcg tgctaagggg cgtgcggagc
    30661 gcaaagctaa aaaacaaagt ttttaaaatt attaaagtct gaaatcagtt cgtttcaggc
    30721 ttttttattg atataaaatc tttatcacct gataaggaaa tactattata cagatagatt
    30781 ttagagtgtt agactagtaa tatgatattt taggagaatt cttatgttta aaaaaataat
    30841 attatctatt gcaactatag ccgcaaccgc cagcctagca gtatcagttc aggcgtcaga
    30901 gaaagtagaa cttaaagtag ctacagattc tgacacggca ccatttactt atcaaaaaga
    30961 cgggaaattc aaaggttatg acgttgatgt tgtcaaagct gtttttaaag gtagtaagta
    31021 caaagtaacc ttcaagacag ttccttttga tactatttca acaggtattg atgcagggaa
    31081 atttgattta tcagctaatg atttttcata taataaagaa agagcagaaa aatatctctt
    31141 ctcagatcct atatcccgtt caaattatgc cgtagtaggg aagaagggga gccattacaa
    31201 atcattaagt gacctctctg gaaaatcaac agaagtttta tctggcgtta actatgcaca
    31261 ggttctagaa aattggaata aaaatcatcc taataaaaaa ccaataaaaa tcaaatatgt
    31321 ttctgggaca actggtgtta ctagcagatt aaaaaatatt gagagtggaa aaattgactt
    31381 tatcctatat gatgccattt catctgacta tattgtaaaa gatcaatcat taaacttaag
    31441 cgtttctcct ttgaaaggta aaattggtaa taataaggat ggattagaat acctcctttt
    31501 accaaaagat aaaaaaggta aaactctaca gaaatttata aataagcgta ttaaagtttt
    31561 gaaagaagat ggtactttgg cacgtttaag taaacaatat ttcggtggag attacgtttc
    31621 aaacattgat aaataaatca gtgagactat aagttttatc tttttacatg tgagagtttg
    31681 cgtgatagaa taacgaagat aagaaagttt agaaaaggaa agtagaagga atgaatcaat
    31741 ttatcttgac gggtggatgg tcttggtata ataatttggt tagtcaggtt ccagcgggga
    31801 aattatttag ttggaaggct gttttagatg caataccctc aattttagag cgattaccca
    31861 taacgctatt attgacagta gcaggagcct tatttggact cattttagct ttgatttttg
    31921 cagtagtgaa aattaaccgt gtcaaaattt tatatccgat tcaagcttta tttgttagtt
    31981 ttttacgtgg gactccaata cttgttcaat taatgctgag ttattatgga attccacttt
    32041 tcttgaaatt tttaaaccaa aaatatggtt ttgattggaa cattaatgct ataccggctt
    32101 ctgtatttgc tattactgca tttgctttta atgaagcggc ttatacaagt gaaactatac
    32161 gagcggcgat tttgtcggtt gatcaaggtg aaattgaggc agcacgtagt ttagggatga
    32221 cctcagcgca agtttatcgt cgtgttatta ttccaaacgc agcagtagtc gcaacaccta
    32281 ctttgataaa cacgttaatt ggtctaacta agggaacctc tttagctttt aatgctggta
    32341 ttgttgaaat gtttgcccaa gcacaaatta tgggtggtag cgattatcgc tattttgaac
    32401 gttatatctc tgtggcactt gtttactggg cggttagttt cttaattgaa caattgggaa
    32461 atgctattga acgaaaaatg gcaattaagg ctccacacca tttaactgat gagattccag
    32521 gaggagtacg ctaatgataa aattgagaca attgactaaa tcattttctg gtcaaaaagt
    32581 gttagacaaa cttgatttag atattgaaaa aggtcaagtt gttgctctag taggtgcttc
    32641 aggtgcagga aaatcaacct ttcttcgtag tatgaattat ttagaagagc ctgattatgg
    32701 aacaattgaa attgatgatt ttaaggttga ttttaaatca atcagtaaag atgacatact
    32761 aacattacgc cgtaagttag ccatggtttt tcaacaattc aatctatttg agcgtcgtac
    32821 agctttggat aatgtaaaag aaggtttgaa aattgtcaaa aagatgtcag atcaagaggc
    32881 aacacggatt gcaagagacg aactggcaaa ggtcggactt gcagatcgtg aaaaatatta
    32941 tccaagacat ttatcaggtg gacagaaaca acgtgttgct ttggcacgcg cattagccat
    33001 gaaacctgat gttcttcttt tagacgaacc gacatcagca cttgatccag aattagttgg
    33061 agaagttgaa aaatctattg cagatgctgc taagcagggg caaaccatgg tgcttgttag
    33121 tcatgatatg aattttgttt accaagtagc agataaagtt ttatttttgg aaaaaggtcg
    33181 tattttagag tctgggacgc ctgaacaatt atttaaccat cctttagaag aacgtacaaa
    33241 agaattcttc gctagttata acaaatcgta cctataagct aaaatatttt gatataatga
    33301 aggttagaat gtctaatctt catttttaat tattcttgag ttaggctaag tatataagaa
    33361 agctgcgata tgttttgaca atcgcttgtt ggatagagat gtttttagaa atagcaaaag
    33421 tttatttaca aggtttaggg cttgccagca taactgttat tttagttggt atattttggg
    33481 tcttttggcg tgccaagaaa aacatagata aaacagctag agaacgccaa tctgttcttt
    33541 atgatgtcct tctgatagct cttatgacag ctccaatact tgcctttgca tttgtagcta
    33601 tcattttaat gataaaagct tagtcagatt agttggaaat atagacgctt tgctatagaa
    33661 taaaaccata agattcaaag gagaaatagt aatgtatgat actttaataa taggttcagg
    33721 tcctggtggt atgacagcag cgctttacgc tgcacgttca aaccttaaag ttgggttaat
    33781 tgaacaaggt gctccaggtg gacagatgaa taatactgct gaaattgaga actatccagg
    33841 ctacgatcat atctcaggtc cagaattatc aatgaagatg tatgagccgt tggaaaaatt
    33901 tgaagtagag catatttatg gtatagttca aagagttgag aacgatggtg atgtaaagcg
    33961 tgttatcacc gaagatgaat cgtatgaagc caaaacagta atccttgcta ccggagctaa
    34021 aaacagcctt ttaggagttc cgggtgaaga ggaatatact agtcgtggcg tttcttactg
    34081 tgcggtttgt gatggtgcat ttttccgtga tcaggatctt ttagttgtgg gtggcggtga
    34141 ttctgctgtt gaagaagctg tcttcttaac tcaatttgct aaaagtgtta ctattattca
    34201 ccgacgtgat caattgagag ctcaaaaagt acttcaagac cgagcttttg ctaacgaaaa
    34261 aataaaattt gtatgggatt cagtggtcaa agagattaaa ggaaatgaga ttaaagtatc
    34321 tggtgtaact gttgaaaatc taaagactgg agaaatctca gagatgactt tcggaggtgt
    34381 tttcatttac gttggtttga aaccacattc tagcatggtc tcagaattag gtattacgga
    34441 cgagactggc tgggtcttaa ctgatacaaa tatgaaaaca tcaattccag gactatatgc
    34501 tattggggat gttcgtcaaa aagatctacg tcagattgcg actgctgttg gtgaaggtgc
    34561 tatcgctgga cagggtgttt ataactatat aactgagcat ttctaagaaa tactaaaagt
    34621 agaaaagact atcgaaaaac gattagtctt tctttttatt ttcatgaaag taaggtgata
    34681 aactgaacta ccgagataga aatatgctat aatgttagac atatagcctt atcctaggct
    34741 catgattaaa aggagattgt ctgatgtata aagacgatag tttaacacta catactgatt
    34801 tatatcaaat taatatgatg caagtctatt ttaataaagg tattcacaat aaaaaagcgg
    34861 tgtttgaagc ttactttcgc aaggttccat ttgaaaatgg ttatgctgtt tttgcaggtt
    34921 tagaacgtat tgttcgttat ttagaaaatc tgagcttttc tgatagtgat ttatcctatt
    34981 tagaagagtt aggttatcct gaagaatttt tagattactt aaaaaattta aaaatggaac
    35041 tgactgtcaa atcagctaag gaaggtgacc ttgtatttgc taatgaacca ttagttcaaa
    35101 ttgaaggtcc tttagcacaa tgtcagttag tagagacagc gattttaaat atcatcaact
    35161 accagacact tgttgctaca aaagcagctc gtattcgttc tgttattgaa gatgaaccgc
    35221 tattagagtt tgggacacga cgtgctcaag aaatggatgc agccatttgg ggtactaggg
    35281 cagcgattat tggtggtgct aacgcaacga gtaatgttcg tgctggtaaa atctttaata
    35341 ttccagtatc aggaacgcat gcacatgctt tagttcaaac ttatggcgat gattaccagg
    35401 catttaaagc ttacgcagaa actcataaag attgcgtctt tttagtggat acttatgata
    35461 cccttcgtgt aggtgtgcct aatgctattc gagttgcaaa agagatgggc gaaaagatta
    35521 atttcttggg agttcgcctt gattcaggtg atttagctta tttgtctaag aaagttcgtc
    35581 agcagttaga tgacgccggt tttcctaatg caaagattta tgcttcgaat gatctagatg
    35641 aaaatacaat cctcaaccta aaaatgcaaa aagctaaaat tgatgtttgg ggtgtcggaa
    35701 caaaattaat tacagcctat gatcagcctg cgctaggtgc tgtttataaa attgtttcta
    35761 ttgagactga cgccggtagt atgcgagata caattaaact atcaaacaat gcggaaaaag
    35821 tatcaacacc tgggaaaaag caagtttggc gtatcaccag tcgagctaaa gggaaatctg
    35881 agggtgatta catcacattt gcagacactg atgtgacaca attagatgaa attgacatgt
    35941 ttcatccaac ttatacttat atcaataaaa cagtccgtga ttttgacgca gtacccttac
    36001 tggtagatat ctttgacaaa gggaaattag tttatcaatt accaagcctt caagaaattc
    36061 aagagtacgg acgcaaagaa tttgatcagc tttgggatga gtataaacgt gttctgaatc
    36121 cacaggatta cccagttgat ttggcacgcg atgtttggca aaataaaatg gacctgattg
    36181 atcgtatccg taaagaagct ttggcaaaag gagaagtaag atgactttgc aagatcaaat
    36241 tatcaaagaa ctcggtgtaa aacctgttat taatccaagt caagaaatcc gtcgttccgt
    36301 agaattttta aaagactatc tattaaaaca tagttttcta aagacctatg tactgggtat
    36361 ttcagggggg caagattcaa ctttagcagg acgtctagct caactagcag ttgaagagtt
    36421 gcgtgctgat acaggggaaa actatcaatt tatagctatt cgtttaccat atggtattca
    36481 ggccgatgaa gaagatgcac aaaaagcatt agattttatc aagccagata ttgctttgac
    36541 cattaatatt aaagaagctg ttgatggcca agttagagct cttaatgcag caggagtaga
    36601 gattacagac tttaataaag gaaatattaa agctcgtcaa agaatgatta gtcaatatgc
    36661 agttgcaggt caatatgctg gagcggttat tggtactgat catgctgcag aaaatattac
    36721 aggttttttc accaaatttg gtgatggagg tgcggactta ttacctctct ttagacttaa
    36781 taaaagtcag ggaaaacagt tattagctga acttggtgct gataaagact tgtacgaaaa
    36841 gattccaacc gcagatttag aagaaaataa accaggaatc gctgatgaaa tagcacttgg
    36901 agtaacctac caagaaattg atgcttatct tgaagggaag gtagtttcgg acaagtctcg
    36961 aggtatcatt gaaaattggt ggtataaggg tcaacataaa cgtcatcttc caatcacaat
    37021 ttttgatgat ttctggaaat aagaaaaata gctagaatat tctagctatt tttgtttctc
    37081 taataaatta ctttcagact tgcagaaata ttcagaatgt ttataatagt agctaagtat
    37141 aaaaggagga atttatgtca gaattaactc aaacatttac agataagtta ttagctgatt
    37201 accaagcaaa tacaaaattt agcgctattg aaaatgcagt aactcacaat ggtttattga
    37261 agtctctaga gactcgtcaa agtgagattg agaacgacta tgtcttttca attgatttga
    37321 ccaaagatga agtctctaat cagaaacaat caggacgttg ctggatgttt gctgcactga
    37381 acacatttcg tcataaactg atttcagatt ttaaactcga gaactttgaa ttatctcagg
    37441 cacatacatt tttctgggat aagtatgaaa aatcaaattg gtttatggaa caaattattg
    37501 cgactgcaaa tcaggaattg agtagtcgta aggtaaaatt cctattagat gtacctcaac
    37561 aagatggcgg acaatgggac atggtggttg ctctgtttga gaaatatggt gtcgttccta
    37621 aaacagttta tcctgaatct gtttcatcaa gtgctagtcg tgaactaaat caatatttga
    37681 ataaactttt acgccaagat gcacagattc tacgagaatt aatagcgcag ggagcagatg
    37741 gggcaactgt tcaaaataaa aaggaagaat tgcttcaaga aatttttaac tttttagcaa
    37801 tgaaccttgg cttgccaccg caatcttttg attttgctta tcgtgataaa gacaatcatt
    37861 atcagtctga taaaaatatt acaccgaaag cattttatca aaaatatgtt aatcttgaca
    37921 tatcagatta tgtttctatt atcaatgctc caactgtaga taagccttat ggacaatcct
    37981 atacagttga gatgcttgga aatgttgtcg gaggcccagc tgttaaatat ttaaaccttg
    38041 atatgaaacg gtttaaagag ttagcgattg cgcaaatgaa atcaggagaa actgtgtggt
    38101 ttggttcaga cgtaggtcaa gtatcaaatc gtcaaaaggg tatcctagca actacgactt
    38161 atgattttaa ttcatctatg gatattaaac ttagccaaga caaagcgggc cgcctagatt
    38221 atagtgaaag tttaatgaca catgctatgg tcttaacggg agtagacctt gatgaatctg
    38281 gacaaccttt aaaatggaaa gttgagaact cttggggaga gaaagttggt aaggatggtt
    38341 attttgtagc ttcagatgct tggatggatg aatatactta ccaaatagtc gttcgtaaag
    38401 aattgcttac taaagaagaa ttagaggctt acaacgcaga gccaattact ttagcgccgt
    38461 gggatccgat gggagcacta gctaattagt aatagtaatt ataaattaaa aaactggata
    38521 tattatccag tttttttaat taccgttagg tgctgtattg ttattaccat taccatgtcc
    38581 agttcctggc acagtttgat tggaattatt aggggtagta gcattaccat tattaggtgt
    38641 ttcgacttgt ggggatgatg aattggcatc atctgctgta ttcttatcct ctttttgttt
    38701 ctcagaggat gttgaactac tgctttgact tgactgtcca gaattttgat agatattctt
    38761 atagacagaa gaagagtaag tgccggttgt agttgtccca ttgatataca agtagcttcc
    38821 acttcgatag agcccttctg gcattgtcca atctgcactg tatccgccag ttaggtagga
    38881 catcattgca cgataaacct cagtagcaat atccagttgg ctaccataaa gtggtgtaag
    38941 gcgattttta taaccagtcc aaattgccat tgtgtactta gaagtatagc cgacaaagtt
    39001 ttcgtcagga gccattgtac caacggcgct attgtagata ccagtagttg cttcaatttt
    39061 agctaactca tcttctgtat agttggaagt accagtctta ccagcttgtg caacaccagg
    39121 gatagctgct ttagtaccag taccaaatgt tagtactgtt ttcaacatat ccgtcatcat
    39181 gtaggcagta gtctctttca tcgcacggct accagacgct gcataagtat cattggttcc
    39241 atcgctaaat tcaattttat taacatattg cggcttatag taagttccgc cgtttgcaaa
    39301 agccgagtaa gccgctgcca ttttctcact acttgcacca tattttgcat cactgctatt
    39361 gttgttactt gaaatagcat ttgaatagtt catttctgga tagtatatgc ctaatttttc
    39421 aaggaaagat tttgcttcgt ctaatccagc ggcttcaagt gctctgacag cagggacgtt
    39481 acgtgattgt tgaatagcgg tctgcatact catccaaccc atatattgac gatcccagtc
    39541 atatagttga gtagaagtac caggccagta gtaaactgag tcgtttaatg attgacctgt
    39601 tgaattatag acaccactat caatagcagg tgcataagct gagataggtt tcattgtaga
    39661 accccaatcg cggtctgtta agacagattg atttgtccca aatgaaatat tttcattctg
    39721 atgacgcccg cctaattgtg caatgacttt accattagtc gcatccatga tggtagatgc
    39781 tatttgtaat tcattgtttg gataagcgat gtaagtatca ctgttgtaga tgtcatatag
    39841 ttgtttttgt gcatcagtgt tgatattagt atacactttt agcccagcag taaagatatc
    39901 tttaccagtt ttttgtttaa cttcactaat aacttgtttt aagtagttat ccatatattt
    39961 tggataagta gatttttgct ttaattcttt taagccatca gttactggag ttgcaacagc
    40021 ttggtcgtat tcctttttag aaatgttttt atcttgatac atctgctgaa gaacggtatt
    40081 acgtcttgtt tgagcagatt ctgggttttt ataagggtca tattgtgtag gtgcttgagg
    40141 aataccagcg agcaaagcaa gttgtgcaat agataattcc tttaggtctt taccaaagta
    40201 tgatttagct gttgttctca taccataatt cccatttccc atataaactt tattaatata
    40261 gaaagtaaga atttcttctt tggtgtattt acgctccatt tgaagcgcaa gccaaacttc
    40321 ctgtgattta cgttttaacg tttggtcaga tttattggta gaaaagtaag ccagtttaat
    40381 caactgttga tcaagggttg aaccaccttg cgtattacta ctaacaaggt tatgccaagc
    40441 tgcacctaaa atacgataaa tatcgacacc tctatgttta aagaaacgtt tatcttctat
    40501 agaagtgata gcgttaacca aatttagtgg aatgctatcc gcactaacac tttcacgctt
    40561 ttctgagcct aaatcggcaa taagtttatt attaccatca taaaccaaac tagagttaac
    40621 ggattttaaa gcttgatcgg ttaatttcgg agaacgacta acgtagtatg caaataggag
    40681 cccacctatt acaatagcta agataataaa tcccattata atgccaaaag catacttcaa
    40741 tagtttgata acagattcct ttttaattgt aatcaccgcc taataatttt tcttcaataa
    40801 tatctaagta agggacttga ggaaaggcac tctcctttac gaaaaatcca ttttttctga
    40861 tataatcaat aggcattgat ttattgcctt tatcaatctg ataaaatgaa attaactcat
    40921 tagcagggag tagataggtt tccttaagtg tggaaaaatg aagcaagaca aagcaaatcc
    40981 ctttttgctg taatacattt gccatgtgct ctatttggtg agcatgaaaa tttttcatag
    41041 gcatagcagt tttctgccgg gtttctttgg cttcaaagtc aatatagtaa cctttgtaaa
    41101 caccagaata atcagtagta gaagcttgtc taaaataagc ttctacaatc ttggcacgac
    41161 tacgtttagg ataatctacc ttgacgattt gtactggcgt tggtttctta tgaatgacag
    41221 ctaattcgtg agacaaatag tagtcattgg ttgcattgat agcagcttca aatgacatac
    41281 cacggttggc aaaatcgatt ttctttttct ttgattttgt aactgttgtt ttccgaataa
    41341 gctgatgtgg atagttaacc atttttctcc ttgcattaca aataaaatgc taagataagc
    41401 aaaactatta taccataaaa ttaggatgga caacaaatga agtcaactat tcttgtaaca
    41461 ggttacaaaa attttgaatt agggattttc caggataagg atcctcggat tactattata
    41521 aaaaaggcta ttgataagga ttttagacgc ttcttggaaa atggagcaga ctggtttatt
    41581 tttatgggaa atttagggtt tgagtactgg gctttggaag ttgctcttga tttacaaaaa
    41641 gaatatgatt ttcaaattgc gacaattttt acctttgaga accatggcca aaattggaat
    41701 gaggctaata aagctaagtt ggcactcttt aagcaagttg actttgttaa atatactttc
    41761 ccaagctatg aaaacccggg tcaatttaaa caatacaatc attttctcat taacaacaca
    41821 caaggtgcat atctttttta tgattctgaa aatgaaacta atttaaaatt tttgctagaa
    41881 atgatggaaa aaaaggaagc ttatgatatt tctttcctaa cttttgatcg cttaaatgaa
    41941 atatatgagg aatgatcgtt ttttcggata tgagaacgtt ttacgcttga tttttccatt
    42001 taatttaggt attatttact ttgatataaa ggataggact tatattaaaa cgatttgact
    42061 ggtttggaga atagaatggc aagtattatt tatagcccaa aagatatttt tgagcaagat
    42121 tttaaagtta gtatgcgggg atatgacaag aaagaagtcg atgtatttct ggatgatgtc
    42181 atcaaagatt acgaaaatta tcttgagcaa attgagaagc tccaaatgga aaatagacgt
    42241 ctacaacaag ctttagacaa aaaagaaagc gaagcttcaa atgtaagaaa ttcagggaca
    42301 gctatgtata atcaaaaacc gattgcccaa tctgcaacca attttgatat tttaaaacgc
    42361 attagccgcc ttgagaaaga agtttttgga agacaaatta gagaataagg atttctcaaa
    42421 tatgcaattt ttggataatc gcgtagtatt gatatactat gaggaaagtc catgctagca
    42481 ctggctgtga tgccagtagt gtttgtgcta ggcgaaaaaa taagcctagg gagatagcta
    42541 gctatcttac ggcaagcaaa agggctaagt ctttggatat gcctgaatag ctttgaaagt
    42601 gccacagtga cgtagtttct agggaaatct agaaagtgga acgcggtaaa cccctcaagc
    42661 tagcaaccca aactttggta ggggcatggg atagttggaa tgagaacaat ctatcctgac
    42721 tgctttgcag tagacagatg attatcgaaa gaaacaattc ctagttgttt ctggaacaaa
    42781 acatggctta tagaaaattg cataataggt tagtggctga gataacatct cagccaattt
    42841 ttaactgtga tgaatacgat agtgtaaaag aggaaaaatg aaagaatcat tcaaattaat
    42901 agcaactgct gctgctggtt tagaagccat tgttggtaga gaaatcagaa acctaggaat
    42961 tgactgccaa gttgagaacg gtagagtccg tttccacgga gacatcaaaa caatcattga
    43021 aacgaaccta tggctacgag ctgccgatcg tataaagatt attgtaggag agtttccagc
    43081 acgtactttt gaggaacttt ttcagggtgt atatggcctt gactgggaaa attatttacc
    43141 gttaggggca aaatttccta ttgctaaagc aaagtgtgtc aagtcaaaat tgcacaatga
    43201 acctagtgta caagccattt ctaaaaaagc tgttgctaag aaacttcaaa aagtctttca
    43261 ccgtcctgaa ggagttccct tacaagaaaa tggagctgaa tttaaaatag aagtttctat
    43321 tttaaaagat aaagcgactg tcatgattga tacgactgga tcaagtctct tcaaacgtgg
    43381 ttaccgagca gaaaaaggtg gtgcgccaat taaagaaaac atggcagcag ctattattca
    43441 attatctaat tggttccctg ataagccatt gattgatcca acttgtggtt caggtacttt
    43501 ttgtattgaa gccgctatga ttggaatgaa tatagctcct ggttttaatc gtgattttgc
    43561 ctttgaggcg tggccatggg tagatcaaag tcaagtacaa aaggttcgcg atgaagctga
    43621 gagtaaagca aactatgata ttgatttaga tatttcaggt tttgatttag acggtcgtat
    43681 ggtagaaatt gctagaaaaa atgcagaaga agcaggactt ggagatgtta tcaaattgaa
    43741 acaaatgcgt cttcaagatt taaaaacaga taaaattaac ggtgttatta tctccaaccc
    43801 accatatggt gaacgactcc tggatgacaa agcagtggac attttgtata atgagatggg
    43861 acaaacattt gcgcctttaa aaacttggag caagtttatc ctaactagtg acgaaggatt
    43921 tgaaaaaaaa tacggctcac aagctgataa aaaacgtaag ctttacaatg gtactttaaa
    43981 agttgatttg taccaatatt acggtgaacg tgtgagacgt caagtaaaat aacggggaga
    44041 actcatgtca gaagatcaaa aacacccatt ttttgaacca gaaaaagaaa ctgatggtct
    44101 tgaatttaaa gatgctaaag aaatgacagt agaagaggct gttagaaaag attctgaaat
    44161 taaggctggt attaccgaag aagatagtat tcttgataaa tacatcaaac aacatcgtga
    44221 tgaagtagct tcacaaaagt ttgagacaaa atcaagcgat tttgcaaacc ttgatacggc
    44281 atctttagat gattttatca agaaacagcg cgaagaatta tcagcgatgt tagctgcaga
    44341 agagctttct aaaaagttgg ataattcagt ctctcaagaa caagatatag aagctaatgc
    44401 tgtctcacct aaagaagaat cttcacaaga acaagaaaat agtgtaactc cagttcctcc
    44461 tctgaatacg gaagcagaac caactgcaac agaaccagat tcgactattg ctgattcaga
    44521 ggagtataaa tcctcttcta agaagcgtgg aggtattgta ggaacattga ttgctcttat
    44581 tttacttcta atagtaacaa tctttggtta caactatttc aagaataata attcgacaaa
    44641 ttctcaaact gcaacaagtc aaagttctag ttctaaggct acgacgactt catcagaaga
    44701 agataaaaaa gctagtcaga acttagataa tttcaacaaa gcttatgcta acttttttgt
    44761 tgatgataag aaaacccaac tcaaaaatag cgaatttgat aaattgtcag aattagaaaa
    44821 gaaagttgat gctttaaaag gaactaaata ctacggtaaa gtaaaagtta aatttgatag
    44881 tctaaaacgt caaattgatg ctgttaaagc tgttaacgac aagtttaagt cgcctgctgt
    44941 agttgatggt aagaagagtg aaaaattaga agtaaaagat ggagcgaatt ttgactcttt
    45001 agatagtaaa actcttaata ctggtaatgc tagtctagat agtttacttc actccatcgt
    45061 ttcaactggt cgtaatcagg taaaacaatc cgaagaacag gctaaaaaaa ctcaagaaga
    45121 acagtcttcg tctaataagg tttcagacac tcaaacaact gaacaaccaa acgtaactaa
    45181 cggtcaatct tcctcatcag ctgctacaat aaataatcag gcagctggca ctgcatcagg
    45241 taatttggaa cgtaaccgta gccgagtacc gtataataat gctgctattg cggacacagg
    45301 aaatcctgcc tggatattta acccaggagt attagagaaa atcgtggcca cttcacaggc
    45361 gcgaggctat ttctctggaa ataactatat tcttgaacct gtaaatatta tcaatggtaa
    45421 tggttattac aatatgttta aattagatgg cacttatttg ttctctatca atgccaagac
    45481 aggttacttt gtaggtaacg ctcctggaag agctgatagt ttagattatt aatataaaga
    45541 gcaaaaccag ttttttgtct acaccctaaa agttagaaaa tcagtctaac gattagggtg
    45601 ttttttgtat tcgcatttag atgtgtacga ctgctatgag gatattaggg aaaaattaaa
    45661 aggattttgc tctctatgat acagaacaaa atcctttgtt taaattaata agacaacttt
    45721 tggaattcca aaagttgact tttatttttt attagatcag acaatgtggc gctcaaaagc
    45781 attgtcagaa atcccttggg aaagaattaa tttcgcccat tcttgagcag agtggagact
    45841 gtggtcttta taatttccac aagattcaat ggttgttcca ggaacgtctt cccaagtgac
    45901 cccaccagca atagcctcta gactagattt gatgacttta gcaatttcag tagcatcctg
    45961 ttttccccac ataatcatgt gaaaaccagt tctacaacca aaaggagaac aatcaatcaa
    46021 accattaatt tgttggcgga ttaacttcgc cagaaggtgc tcgatagtat gtaaaccagc
    46081 agtatcaata gcattttcat taggctgaat aaggcggata tcaaagttag taatgatgtc
    46141 tccgaccggt ccaacttctt ctgaaattaa acgaacgtag ggtgctttaa cgatggtgtg
    46201 atctaattca aaactctcta cgacaacttc ttttgtcata agttttctcc ttttgtatta
    46261 cattctagag actaaaggag atgttgaaaa tttctcctaa tccctttaaa taacattcta
    46321 atcatatcat aaatgtctat aattttaaat tgtgaaaacg ccttttttat gataaactta
    46381 aaagtggact ttctatatga aatctagaaa aaaattgagg tataaacatg tttaatatta
    46441 tattagcaat ggtttgtgca ctcattggtt tgattattgg ttatgtggct atttcaatga
    46501 aaatgaaatc atcaaaagaa gctgcagaac taactctttt aaatgcagaa caagatgccg
    46561 ttgatttacg tggtaaagca gaaattgaag cagaacacat ccgtaaagca gcggaacgtg
    46621 aaagtaaagc tcaccaaaaa gaattacttt tagaagcaaa agaagaggct agaaaatatc
    46681 gtgaagagat cgaaaaggaa tttaaatctg atagacaaga gttgaaacag atggaggcac
    46741 gtttaacaga tcgtgcatca tctcttgatc gtaaagatga aaatctatct aacaaagaaa
    46801 aaatgttaga tagcaaagaa caaagtctaa ccgataaatc tagacatatt aatgagcgcg
    46861 aacaggaaat tgctacttta gaaacaaaaa aagtagaaga attgtcacgc atcgcagagc
    46921 ttagtcaaga agaagctaaa gatatcattt tagctgatac cgaaaaagat ttagctcatg
    46981 atattgcgac acgtattaaa gaagcagaac gggaagtaaa agatcgatca aacaagatag
    47041 caaaagactt gctagcccaa gctatgcaac gtttagctgg tgaatatgtt actgaacaaa
    47101 caattacaac tgttcatctc cctgatgata atatgaaggg acgtatcatt ggtcgtgaag
    47161 gacgtaatat tcgtactctg gaaagtttga caggtattga tgttatcata gatgatacgc
    47221 cagaagtcgt tgtcttatca ggtttcgacc ccattcgtcg tgaaattgct cgtatgactt
    47281 tagaatcttt gattcaagat ggacgtatcc atccagctcg tattgaagag ttggttgaaa
    47341 aaaatcgttt ggaaatggat caacgtatcc gtgagtatgg tgaggcagca gcttatgaga
    47401 ttggtgcacc aaatctgcat cctgatttga taaaaattat gggacgtttg cagttccgta
    47461 catcatatgg tcaaaatgta cttcgtcatt cagttgaagt tggtaaactt gcaggtattt
    47521 tagctggaga actaggagaa aatgttgatt tagctcgtcg ggctggcttc ctccatgata
    47581 tggggaaagc tattgatcgc gaagttgaag gcagtcacgt tgaaattggt atggaatttg
    47641 ctcgtaagta caaagagcat ccgattgttg ttaatactat tgcaagccac catggtgatg
    47701 tagaacctga cagcgttatt gcagtaattg ttgctgctgc agacgccctt agttcagcac
    47761 gaccaggtgc gcgtaatgag tcaatggaaa attatatcaa acgtctgcgt gatttagaag
    47821 aaattgctaa tggctttgag ggagtgcaaa atgcctttgc acttcaagcc ggacgtgaaa
    47881 ttcgtatcat ggtacaacct ggtaaagttt ctgatgatca agtagttatt atgtcgcata
    47941 aggtacgtga aaaaatcgaa caaaacttgg attatccagg taatattaag gtaacagtta
    48001 ttcgtgaaat gcgagctgtt gattttgcaa aatagtcaaa aggagagctt gttagctctc
    48061 cttttgttat agagttgaca tttgtcatat tttgaaatga caaaaatatc taaaaagttt
    48121 ccttctgatt ttttatacta tgattatagt aaaggagaac caaatggcaa aagatataat
    48181 taaggttgat catatattta aaagcatcgg gcaaaaaacg attttagaag atatatcatt
    48241 tagtatagct tctaaccaat gtgtagcatt gattggacct aatggtgcag ggaaaacaac
    48301 tctgatgtca actttattag gtgatattag tatttcttca ggagcattga ctatttttaa
    48361 tctgcctgca caccacaata ggttaaaata taaggtggcc atcttaccac aagaaaatgt
    48421 attgccctca aagtttacag tgagagaact cattgatttc caaaggtgtt tatttccaga
    48481 agtgctgcca atgtctctga ttttagacta tctacagtgg agtgatgcgc acttgcaaca
    48541 atttacagag actttatctg gagggcaaaa acgactttta gcttttgttt taactttggt
    48601 tggtaagccc caattactat ttttagatga acctacttcc ggaatggata cttccacacg
    48661 tcaacgattt tgggagctgg ttgcgacact aaaaaaagaa ggtgacacaa ttgtctattc
    48721 tagtcattat atcgaagagg tagaacatac agctgatagg attttagtac ttcataaagg
    48781 aaagttatta cgcgatacaa ccccctttgc catgaagcaa gaaaaaaccg aaaagttatt
    48841 caccgttccg cttagttatc aaaaattatt acctacctat ttgattacag agtgtgaagc
    48901 caagagtgat agtataacgt ttgttactgg ggaggctgaa actgtatgga aaatactggc
    48961 agataatggt tgtcctattg aagctattga gatgaccaat agaactttgt taaatcgtat
    49021 ttttgagact actaaggagg taaaacatga gaatctttaa agcattattg aaaatggaat
    49081 ttcttttgac aaaacgtcaa cttgccaatc tcattatggc tataggtatg ccagtggctt
    49141 tttttctatt tttttcagga tttatgggcg aaggactcac taaagccatc gaagctatct
    49201 acgttcgtaa ttatatgata acgatggcag gattctcaag tcttagcttt gcttttttta
    49261 cttttccatt ttctatgaaa gatgatcaac tctcaaaccg aatgcaatta ttaagacact
    49321 caccagtacc aatgtggcaa tattatctag caaaaattat tcgtatttta ttttactatt
    49381 gcctagcaat aactgttgtt ttcctaacag ggcatatttt aaggcaagtt agtatgccaa
    49441 tagaacaatg gatgcagagc tttttattgc tattaggagg agcaacatgt tttattcctt
    49501 ttggattgct cgtctcctac tttaaaaata ctgaactaat gtcaatggta gccaatattt
    49561 gttatatgtc attagctgtt cttggtggca tgtggatgcc aattacaatg ttcccaaaat
    49621 ggttgcaagg tttatcaaag ttaaccccta cttaccattt gactcaagtt atccttagtc
    49681 cgtttgctaa tagttttgct gttttttctc ttattattct gataggctat ggtattataa
    49741 tgttagttat cgcttatcta ctatctcaga agaggcattc aatttgaaaa ataaaaaaaa
    49801 taactatgat ttaatgtatt ttattccgtt agtatttttg atttacccca taggagggat
    49861 tctctattat cactaccctt tttggacttt attttttacg ctagcttttg taggagcata
    49921 tttatactct attattatca ggggcgaatc aaagtatcac atgattgcct ggtttacaat
    49981 gttgacatat attttctaca tgacagtttt tataaatagt ggtttcatct ggtatattta
    50041 ctttctgagt aatttactcg tctatcgctt tagagataaa ttgaagtctt ttagattcat
    50101 tagttttgct tgcactctag ccacaatggt atttttatgc ttctttaagg catcagactt
    50161 tggtgataga atcatgtttc taattgttcc tattttttgt atcggttaca tgtggattgc
    50221 tattgaaaat agaaatagcg aagaacaaag agaaaaaatt gcagagcaaa atcagtatat
    50281 caatattttg tcagctgaaa atgaacgtaa ccgtataggg cgtgatttgc atgatagttt
    50341 aggacataca tttgctatga tgacactaaa gacagaacta gcgttaaagt tgttagaaaa
    50401 aaggaactat gataaggtac aaaaagaact atcagaatta aatcatatta gtcatcaatc
    50461 gatgagtgag gtccgtcaaa ttgtttctaa cctcaagtat cgtacagtag tggaagaaat
    50521 tgatgagttg taccgcttgt ttcaattatc taacatcaag ttaacagttg ttaataagtt
    50581 agagacgtca caactaagtc cagtaacaca atctacaatc acaatgatat taaaggaatt
    50641 atccaataat attgttaagc atgctgaagc tgatagcgtg gagttgagtt tagtaaggca
    50701 aggtgctact ataaatattg agatgattga taatggttgt ggttttacaa accttgacgg
    50761 agatgagttg catagtattc aagagagatt aactatcgtt gaagggacac taacgatttt
    50821 gtcccgaagt aaaccaactc atattcaggt gatattgaaa gaggtataag atgaaattac
    50881 ttgttgctga ggatcagtcc atgctacgag acgctatgtg ccaattactt ttgatggaag
    50941 aaagtgtttc tactattgat caagctggaa atggtgaaga agctattgcc attttatcca
    51001 ataaagccat agatgttgca atattggatg ttgagatgcc gatattatca ggtttagatg
    51061 tcttagagtg ggttagaaaa taccagaatg ttaaggtcat aattgtaaca acctttaagc
    51121 ggtcaggtta tttccaaagg gctattcgtt caaatgtaga tgcttatgtt ttgaaagacc
    51181 gctcagtagc tgatttgatg aaaacgattc aaaaagtttt atctgggggt aaagaatatt
    51241 cgcctgaatt aatggaaaac gttatatcta atcctttgag taagcaagaa ataaaaattc
    51301 tttccctaat tgctcaaggg aagaccagca aagaaattgc atgcacttta tttttatcga
    51361 atggtacagt tcgtaattac acgtcaaata tttttgataa attaggagtt aataatcgtt
    51421 tggaggcact taaaatagca cgagaaaagg attggctgtg atagtttttt tccagtcgag
    51481 atgccataat gtattaagag ggatatatgt atgcaaaaat ttaaagaacg ttttgatacg
    51541 cttagtgatg caattttggc cattgctatg acgattttag ttttggagat aaaaactccg
    51601 actacgatgg gtgatattgg tgactttaca cggaacatag gtttatttat tgttagcttt
    51661 gttgttgttt ttaatttttg gtacgaaagg gcacaaaata gcctagatgc tcaaaagact
    51721 aatgatgaaa ttatagctct tgatatcatt gaacacctag gtatttgttt gattcctctc
    51781 tttactaaat ttatgatttc ttttgagaat cataattttg ctgttatggc ttatggactc
    51841 ttaacgttat tagttggtct cacatcagac attattcgga ttcgtttggc aagctatgat
    51901 ttagtgacca ttcctagtga attgaaagag cgggttataa aagtcatgac aacttttgcc
    51961 attcgttcag ttgtggttcg ctttatcatt attatcctcg cttattttct acctgaggta
    52021 gggatatttg catatttagt cataccgctc tttatgtttt ggcgtcgctc tagagcagga
    52081 aaatactata ctaaaaaggg cattgaggca ccctcttata tcaagctttt tatccaaatt
    52141 ggagagagag ttcctgagga ttttactaat tcaagtagtg gaataagtgg ggtaaaaaga
    52201 ctctataaaa aataatagga gaccttgaaa aaaaggtttc tttttgttaa actaatgaga
    52261 agaaacttta tttagggagc aatttatgaa ttgctataaa agttaatagt taaggtaaga
    52321 accagattgt tctggtcttt ttaacactta acacccgatc tattaatttg aggagtgtgt
    52381 aatgtctgaa cgtggtttgc taattgtttt ctcaggacca tctggtgttg gtaaggggac
    52441 agttaggcaa gaaatttttt caacccctga ccataaattt gattattccg tttcaatgac
    52501 tactcgtcca caacgcccag gcgaagttga tggagttgat tatttcttcc gtacacgtga
    52561 agagtttgaa gcgttgatta aagaaggtca aatgcttgag tatgcggagt atgttggtaa
    52621 ctactatgga actcctttaa gttatgtcaa cgagacttta gacaaaggga ttgatgtttt
    52681 tcttgaaata gaagttcagg gagcgcttca agttaaatcg aaggttccgg acggtgtttt
    52741 cattttctta acaccaccag atttagagga attagaagag cgtttagtag gacgtgggac
    52801 agatagtcca gaagtgattg cccaacgtat tgaacgtgcc aaagaagaaa ttgctttgat
    52861 gagggagtat gactatgctg ttgtcaatga tcaagtgtca ttagcagcag aacgtgttaa
    52921 acgtgtcatt gaagcagaac attatcgtgt ggatcgtgtt attgggcgtt ataccaacat
    52981 ggtcaaagaa actgataaaa aattaagtta aaaatagaaa agagaattgc cttatgatgt
    53041 taaaaccttc tatcgacact ttgttggata aagttccttc aaaatattct ttagttattt
    53101 tacaagctaa gcgtgctcat gaattggaag ctggtgagaa agccactcaa gattttaaat
    53161 ctgtgaaatc aacacttcgc gctttagaag aaatcgagtc aggtaatgtt gtaattcacc
    53221 cagatccatc agcaaaacgt gcctcagttc gcgctcgtat cgaagctgaa cgccttgcta
    53281 aagaagaaga agaacgtaaa atcaaagagc aaattgctaa agaaaaagaa gacggcgaaa
    53341 aaatttaagc aagtcaaaag tgagaggttg aaacattctg tatcagcctc ttttcatatt
    53401 ttagagaatt agggagggtt tatggcaaga aaattagcac aggttattgt tgatattcct
    53461 ctgatgcaga cagataaacc attttcgtat gccattccta aagatttgga agatttggta
    53521 caagtaggag cacgcgttca tgttcctttt ggaagaggga atcgtctttt gcaaggcttt
    53581 gttgtcggtt tcagagatga tgatgaatta gagactaagg atattgcggg ggtcctagat
    53641 tttgaacctg ttttaaatca ggaacaacta gacttagctg atcagatgcg ccatactgtc
    53701 ttttcttata aaatatctat tttgaaatca atgttaccaa gtcttttgaa ttcgcaatat
    53761 gataaactct tgttagcaac agacacatta ccatcagaag atagagaaga tctatttggt
    53821 cataaaacgg aaattgtctt ttcaagtttg tcttctcaag atgccaaaaa agcaggcaga
    53881 ttaattcaaa aaggtttcat agaagttcaa tatttagcca aagataagaa aacaataaaa
    53941 acagaaaaga tttacaaaat caacagaact ctgttagaag agagtcaaat agcagcaaga
    54001 gcgaaaaaga gactggagtt gaaagaattc ctgttagaaa atcctcaacc agggcgcctc
    54061 acagctttaa acaaacaatt ttcatctcct gtagttaatt ttttcagaga agaaggaatc
    54121 attgaggtca ttgaaaaaga agctagtaga tctgataatt attttaaagg aatcctaaaa
    54181 acggattttt tggatttaaa tcaggaacaa gctaaagtag ttaaaatagt tgttgaccga
    54241 attggtaagg atcagaataa acccttcttg ttggagggga ttacagggtc tggtaagaca
    54301 gaggtatatc ttcatgtcat tgataacgtt ttaaagttgg gaaaaacagc gattgttctc
    54361 gttccagaaa tttctttaac tccccagatg acgaatcgtt ttatttcccg ttttggaaag
    54421 cgagttgcta ttatgcactc gggcttgtca gatggtgaaa aatttgatga gtggcgaaag
    54481 ataaaatcag gtcaagctaa agttgtcgtt ggagctcgtt cagcaatttt tgctcctttg
    54541 gaaaatatcg gagccatcat tatagatgaa gaacatgaat caacttataa gcaagaatct
    54601 aacccaaggt atcatgcgcg cgatgtggcc ttgctacgtg cagaatatca taaagctgtg
    54661 ttactaatgg gatcagcaac tccaagtatc gaaagccgag ctagagctag tcgggatgtt
    54721 tataaatttt tagaattaaa acatcgtgct aatcctaaag cacgtattcc tcaggttgaa
    54781 attattgatt ttagaaattt tattggtcaa caagaagtta gtaatttcac accatattta
    54841 ctagataaaa tccgagatag attagataag aaagagcaag tggttcttat gctcaatcgt
    54901 agaggttatt ctagttttat tatgtgtcga aactgcggct acgttgatca atgtcctaat
    54961 tgtgacattt ctctgacttt acatatggct acaaagacca tgaattgtca ttattgtggg
    55021 tttgaaaagc ctattccaag aacatgtcca aattgcaata gtaaaagtat ctcttactat
    55081 ggtactggaa cccaaaaagc ttatgaagaa cttttgaaag ttatcccaga cgcaaagatt
    55141 ttacggatgg atgttgatac gacaaggcaa aaaggcggtc atgagtctat tttgaagcgt
    55201 ttcggaaatc atgaagctga tattttattg ggaacacaga tgattgctaa gggattggat
    55261 tttccaaatg ttactttagt tggagtacta aatgcagata cttctctgaa tttacctgat
    55321 tttcgatcat cagagcgaac ttttcagcta ttaactcaag tggctggacg tgctgggcgt
    55381 gctgaaaaag aaggagaggt tgtcattcaa acttataatc ctaatcatta tgctattcaa
    55441 ttagcccaaa agcaagattt tgaagctttt tatcaatatg aaatgaatat ccgtcgacaa
    55501 cttggttatc ctccttatta ttttacagtt ggtttaacat tatcgcataa agatgaagag
    55561 tggttaatac gaaaatctta tgaagtatta agtttattga agcaaggctt tagtgataaa
    55621 gtaaagttgt taggtccaac gccaaaacca attgcaagaa cacataacct ttatcactac
    55681 caaattatta tcaagtatcg atttgaggac aaccttgaac ttgttttgaa tcgcttatta
    55741 gacatcactc aagacaagga gaatagagat cttcgcttag caatagacca cgaaccgcaa
    55801 aatatgatgt agaatagtgc tatgatataa ttgaaaaaag aaagacgagg aaaaagcaat
    55861 gacaaaatta ttattcatgg gtacccctga tttttcagcg acggttttaa aaggtatttt
    55921 agcagatggt aagtatgatg ttttagctgt tgtgacgcaa ccagatcgtg ctgtaggtcg
    55981 taaaaaagaa attaaaatga cccctgtaaa agaagtggcc ttggaaaaca acattcctgt
    56041 gtatcagccg gaaaaactat ctggaagtcc agaattagag caactaatga ccctaggtgc
    56101 agatggtatt gtaacagctg cttttggtca attcttacca acaaagcttt tggaatcggt
    56161 aggttttgca attaatgtac atgcttctct attaccaaaa tatcgtggtg gggcaccaat
    56221 tcattatgcc attattaatg gcgaaaaaga agctggggtt accatcatgg agatggttgc
    56281 aaagatggat gcaggggata tggtatcaaa agcttccgtt gagataactg atgaagacaa
    56341 tgtagggaca atgtttgata gactcgccgt tgttggtcgt gaccttttat tagatacgct
    56401 tccaggttat ctatcaggtg atattaagcc gataccacaa aatgaagaag aggtaagttt
    56461 ttctcctaat attagtccag atgaagagag aattgattgg aataaatcat cgcgcgacat
    56521 ttttaaccat gttcgaggga tgtatccatg gccagttgca catacccttt tggagggaaa
    56581 tcgttttaaa ctttatgaag taacaatgtc agaaggaaag ggctcaccgg gtcaagtgat
    56641 cgctaagaca aagaactcat tgacagtagc aactggtgac ggagctattg aactcaaatc
    56701 agttcaacca gcagggaaac ctcgtatgga tataaaagac tttttaaatg gtgttggtcg
    56761 taatttagaa ataggagata aatttggcga atgattggaa aaagagtgcg cgtggtttag
    56821 ctttaatgac tttagaagaa gtttttgata aaggagctta ttctaatatc gcacttaata
    56881 agagtttaaa aaaatctaga ctatcagata aagatcgtgc cttagtaaca gaaattgtct
    56941 atggaactgt ggctagaaaa ataacgctag aatggtatct atcacacttt atagttgatc
    57001 gtgataagct tgaattatgg gtatatcatc tcttgctact gagtctttat caactactct
    57061 atctggacaa catcccagat cacgctattg ttaatgatgc tgtcactatt gctaagaatc
    57121 gaggcaataa aaagggtgct gaaaagttaa ttaatgccgt tttacgaaga gtaagttcgg
    57181 agacgctccc tgaaattgca tcaataaaac gccaaaacaa acgttactct gttgcttatt
    57241 ccatgccagt gtggctcgta aaaaaattaa ttgaccaata tggtgagaca agagctttag
    57301 caattatgga gagtttattt gagcgaaata aagccagtct tcgtgtgact gacttatcgc
    57361 aaaaacaaac tattaaagaa actttaaatg ttagagattc acatattgca gagacagctt
    57421 tagttgcaga ctcaggaaat tttgcatcga catcattttt ccaagatggc ctcattacta
    57481 tccaagatga atctagtcaa ttagttgctc caacactcaa agtctcgggt aatgaccaag
    57541 tattggatgc atgtagtgca ccaggaggaa aaacaagtca tatcgcctca tatttgacta
    57601 caggggcagt aactgcttta gatttgtatg atcataaatt ggaattggtt atggaaaatg
    57661 caaaacgcct aggtctgtca gataagataa aaactaaaaa gttagatgca agtaaagctc
    57721 atgagtattt tcctgaggat acttttgaca aaattttagt agacgcacct tgttcaggta
    57781 tcgggctaat tcgccgtaaa ccagatatta agtacaataa agctaatcaa gactttgaag
    57841 ccctccaaga gattcaattg tctatcctat ctagcgtttg tcaaacgcta cgtaaaggtg
    57901 gtataataac ctatagcact tgtaccattt ttgaggaaga aaattttcaa gttattgaaa
    57961 aattcttaga aaaccacccc aattttgagc aagtagaact gagtcataca caagaggata
    58021 ttgtaaagag aggctgtatt agtataagtc ctgaacaata tcatacagat ggctttttta
    58081 ttggacaagt caaacgtatc ttgtagtgtc taggaggaaa ctgacatcgt cagaaaaaaa
    58141 tatatggaaa tatctctttt aacagatatt gggcaacgtc gttccaataa tcaagatttt
    58201 attaatcaat ttgaaaataa agccggcgtc ccactaatca tcttagcaga tggtatggga
    58261 ggacatcgtg caggaaatat tgctagcgag atgactgtaa ctgatctagg aagtgattgg
    58321 gcggagacag atttttctga gttatcagaa attcgtgatt ggatgcttgt ctctattgaa
    58381 actgagaatc gcaaaatata tgaattaggt cagtcagatg attacaaagg tatgggaact
    58441 accattgaag cagttgccat tgtaggagat aatattattt tcgcacatgt aggagattca
    58501 cgtatcggta tcgttcgtca aggggagtat catttattga cgagtgatca ttctcttgtt
    58561 aatgaattgg taaaagccgg ccaattaaca gaagaagaag ctgctagtca tcctcaaaaa
    58621 aatattatta cgcaatcaat tggtcaagcc aatccagtcg aacctgattt aggagttcat
    58681 ctacttgaag aaggtgacta ccttgtagtt aatagtgatg gactaactaa tatgttgagt
    58741 aatgccgata ttgctacagt actaacacaa gaaaaaacct tagatgataa gaatcaagat
    58801 ttaatcactc tagcaaacca tagaggaggt cttgataata tcactgtggc actagtttac
    58861 gttgaaagtg aggcggttta atgattcaga ttggcaaatt atttgctggt cgttatcgga
    58921 tccttaaatc aatcggacga ggtggaatgg cggatgttta tctagctcga gatctaattt
    58981 tagataatga agaggtcgca ataaaagtat tgcgaaccaa ttatcaaaca gatcagattg
    59041 cggttgctcg cttccagaga gaagcacgag caatggctga gctgacacat cctaatattg
    59101 tcgctattcg tgatataggt gaagaagacg gacagcagtt cttagtaatg gaatatgttg
    59161 atggattcga tttaaaaaag tatattcaag acaatgcacc cctatcaaat aatgaagttg
    59221 ttcgaatcat gaatgaagtc ctttcagcca tgtcactggc acatcagaaa ggtatcgtac
    59281 accgtgattt aaaacctcaa aatattttat taactaaaaa agggactgta aaagttacag
    59341 atttcggtat tgcggtagcg tttgctgaaa ctagtttaac gcaaacgaat tctatgttag
    59401 gtagtgttca ttatttatcg ccagagcagg cgcgtggttc aaaagcgact gttcagagtg
    59461 atatttatgc catgggaatc atgctttttg aaatgttaac tggccatatt ccttatgatg
    59521 gggatagtgc agttactatt gccctacagc acttccaaaa accactacca tcaatccttg
    59581 cagaaaataa aagcgttcca caagctttgg aaaacattgt cattaaagca acagctaaaa
    59641 agctaacaga tcgctataag acaacttatg agatgggacg tgatttatca actgctctaa
    59701 gctcgacacg tcaccgtgaa ccaaaacttg tttttaatga tactgagagt actaagacac
    59761 taccaaaagt aacatctaca gtatcatctt taacgacaga acaactgttg cgtaatcaaa
    59821 aacaggccaa aacaacagaa aaaataactc ctgattctgc gagtaacgat aaaacgaagt
    59881 ctaaaaagaa ggcttctcat cgacttcttg gtacaatcat gaaattattc tttgcactct
    59941 gtgttgtagg aataattgta tttgcttata aaattttagt aagtcctacg actattcgtg
    60001 ttccagatgt tagcaacaaa acagtagctc aagcaaaaat gacattggaa aattctggat
    60061 tgaaagtagg agctattcgt aatattgaga gtgatagtgt ttcagaaggc cttgtcgtaa
    60121 aaacagaccc tgctgcagga cgttctcgta gagaaggggc taaagttaat ctttatattg
    60181 ccactccaaa caagagtttc actctaggta attataagga acataattac aaagatatcc
    60241 tcaaagatct tcaaggcaaa ggtgttaaaa aatctttaat caaggttaag cgtaaaatta
    60301 ataatgatta tactactggt acaatcttag cacagtcgct tccagaagga acatctttta
    60361 atcctgatgg taataagaaa ttaacactta cggttgctgt taatgatcca atgattatgc
    60421 ctgatgtaac aggtatgaca gttggagaag taatagaaac cttgacagac ctcggattag
    60481 atgcagataa tcttgtcttc tatcaaatgc aaaatggcgt ttatcaagca gtagtaacac
    60541 ctccatcttc aagtaagata gcaagtcagg atccttatta cggaggagaa gttggattaa
    60601 ggagaggaga taaagttaag ctgtatcttc ttggttctaa aacaacaaat aactcatcat
    60661 ctacgccaat agattcttcg gcttcaagct caacaggaac aactacctcg gattctgtaa
    60721 gctcatcaac ggatgctagc acttctgata gtagtagcac ttctacaagt agttcaacac
    60781 taccaagtga tagtacaatt aatacaggaa cagctaacaa tccattaact caataaaatt
    60841 aaaaagaaga tgttaaagtc ttctttttaa tttgctatgg tagagaccat gcctagaaag
    60901 gttctgccaa tatgccaaat ttttctctca ataatggtgc aaagtatagg gctttttgat
    60961 aaaatacgtc atatagagta atggaaggat tattatgaaa aaatttcagt tttttctact
    61021 tgttgaagcc gttgtcttag ttatgggatt aatgaaaatt ttatcagatg actggacaag
    61081 ttttattttt attctcgctt tgattctttt agccttaaga ttttataaca acgatagtcg
    61141 tcataatttt ctgttaacga caagtttgtt attactattt ttaatcttta tgcttaaccc
    61201 ttacattatt gcagccgttg tttttgcagt tttgtatgtt ctaatcaatc atttctcgca
    61261 agttaagaaa
//
LOCUS       DATRUK010000003        57635 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.003, whole genome shotgun sequence.
ACCESSION   DATRUK010000003 DATRUK010000000
VERSION     DATRUK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 57635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57635
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.003"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..124
                     /locus_tag="VBE34_000115"
     CDS             <1..124
                     /locus_tag="VBE34_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HEO7262874.1"
                     /translation="KSVININLQDLKNKEGIYLKATAIDLVNDNFSFDIFKKKP"
     gene            227..502
                     /locus_tag="VBE34_000116"
     CDS             227..502
                     /locus_tag="VBE34_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEO7262875.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFAAVADYLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            complement(590..1732)
                     /locus_tag="VBE34_000117"
     CDS             complement(590..1732)
                     /locus_tag="VBE34_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7262876.1"
                     /translation="MWSEKHKSGKVNFVERYKDPYTNKWKRTSVLMEKDTPRIRKEAQ
                     RILDAKIADIVRKLQTSDMLFTNLIDEWWIFYQQEIKRSSIVTLKGNIREIRAEFGIN
                     IPVVNIDPRYVQNYLDNLDCSRNKKERNKSMLNLIFDYAVSLDIIKDNPARRAKLPKI
                     KKTLNDWKKIEEKYLEEEEIKRLLKELFRRPSTHRLGFLSEFMSLNGCRIGEAISIEP
                     DNIDFKNKTLQLHGTYDRTNGYINGEKTSPKTLASYRETIMSKREMEILQEMEFINEL
                     EKNTNKRYRDMGYIFTTRNGVPIQINSFNLALKKANERLEQPINKNITSHIFRHTLVS
                     RLAENNVPLKAIMDRVGHADAKTTVQIYTHITKKMKSNIADIMENY"
     gene            complement(1847..2398)
                     /locus_tag="VBE34_000118"
     CDS             complement(1847..2398)
                     /locus_tag="VBE34_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262877.1"
                     /translation="MDINKIKEFAVSTSEKAKDGIIKANEMRKRASQESKIVLPPTIN
                     LGFNTPTAIRKTVEGDYYIGFYSEKPDLFKFVDFQFDGSRIIEKTVTKGKTTQKGRSG
                     STIIGGLIGATINPTAAVLGGLAGANRGKKGTINSTSTTITEEIPGKAQLTLRNLKTN
                     DIKIIKTKLTQAQANNIQNFFSK"
     gene            complement(2416..2802)
                     /locus_tag="VBE34_000119"
     CDS             complement(2416..2802)
                     /locus_tag="VBE34_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO7262878.1"
                     /translation="MTIEEIVDCHKVNLAYFDNELWPRPGIYIDDIKVIFVNKSLSLD
                     AQKRVILHELGHCERNIGDYKTLREKHELEADRYMISHLIAEALSQLEDKREFNYLQF
                     MQYHQLTTTADECMVIDEYYNLLKVV"
     gene            complement(2806..3153)
                     /locus_tag="VBE34_000120"
     CDS             complement(2806..3153)
                     /locus_tag="VBE34_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7262879.1"
                     /translation="MFPTFEKIKELCKQRGITLIQLEETLGYSRNTLYKLKTQKPNAE
                     RIAEIADYFNVSTDYLLGRTDNPNIAKDSQEYTSDDLRKMAEKAKTFDGKPLTESDID
                     AIQNIIEIYLKGR"
     gene            3449..3694
                     /locus_tag="VBE34_000121"
     CDS             3449..3694
                     /locus_tag="VBE34_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262880.1"
                     /translation="MKKNIVDLQKRNEHFQWVADSLEGKENELYVERDWYDNPTLISK
                     EDAKKEVEQVQQKLILLQKKSFIEYILQLLHQLFRRQ"
     gene            complement(3645..4433)
                     /locus_tag="VBE34_000122"
     CDS             complement(3645..4433)
                     /locus_tag="VBE34_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262881.1"
                     /translation="MSRYSDYKYANTILKSIEERPEKYLIIHYSCESFYNLGSKSPRI
                     ASISVRQFNNAQTNNFSIHQYSEILDTPITDDTYETIEKELLTDFFTFVDRNTDKNWI
                     HWNMRDTVFGFHAIEQRFKVLGGTPIIIDDDKKIDLAYLFKKMYGGGYIDNPHIEKLL
                     ELNNLTPHRFLTGKQEADAFSSRQYHELSMSTSSKVNSFSTFLTLAINDDLKTNTPKW
                     KMRGTNLSGLLSAFQETTSGKVIIGIINLIVGGIVGAIIAKYIQ"
     gene            4484..4675
                     /locus_tag="VBE34_000123"
     CDS             4484..4675
                     /locus_tag="VBE34_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO7262882.1"
                     /translation="MPDIAVGREKVVTFLKSNNIKKSDLAAAYGLNRQEVTNILSGST
                     RGPKANQFILRVIADYSIE"
     gene            4755..5066
                     /locus_tag="VBE34_000124"
     CDS             4755..5066
                     /locus_tag="VBE34_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO7262883.1"
                     /translation="MPKAELVYRPAKQSEKADYGDYVHLCQIWEGLTVGTAKVWAAEM
                     RNHPDFKQFINNPTHRIVFINYEGFKLFVTWKSRNRYKPKKETLAEMLENIKKERQLG
                     V"
     gene            5071..5220
                     /locus_tag="VBE34_000125"
     CDS             5071..5220
                     /locus_tag="VBE34_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262884.1"
                     /translation="MKKLFKKLFVKKNQVIETRQKWTIETHGWEANARRYDNMIERNN
                     RGRTC"
     gene            5214..5441
                     /locus_tag="VBE34_000126"
     CDS             5214..5441
                     /locus_tag="VBE34_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262885.1"
                     /translation="MLKEVLLENELLRDENRRLTNEMADYYFTNVAKANLLDIIVNEG
                     YILDSTLEKCIAKLDNVDRQTLEKNWSDDND"
     gene            5434..5664
                     /locus_tag="VBE34_000127"
     CDS             5434..5664
                     /locus_tag="VBE34_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262886.1"
                     /translation="MIEVERQPKNTYSRIRCSNNIYDEIANIANECDLTLKEVTDALL
                     NYGLANAQIISSEKVVTESKLVIGDFDNDNHN"
     gene            5648..6967
                     /locus_tag="VBE34_000128"
     CDS             5648..6967
                     /locus_tag="VBE34_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO7262887.1"
                     /translation="MTITINKLEIENVKRIKAVKVEPSSKGLTIIGGNNNQGKTSVLD
                     SIAWVLGGNKFKPSQAAREGTMVPPTLKITMSNGLIVERKGKNSSLKVIDPNGQKGGQ
                     QLLDSFVEELAINLPKFMDGTPKEKADVLLEIIGVGDQLAELELKEKELYNQRHTIGV
                     IADQKEKFAKEQPYYNDAPKELISIADLIQQQQEVLAKNGENARKRQNLSVIQQNHQF
                     KQSEVEHLKQQLATAEKQLQELSSDLEIAQRDTMSLIDESTAEIEENISNIEEVNRKV
                     RANLDKDKAEEDAKHQREQYNILTNDIESIRQQKRDLLINADLPLEGLSVDDGKLLYL
                     GQEWDNMSGSQQLIVATAIVRKLKPDCGFVLIDKLEQMDNITLEQFGKWLEQEGLQAI
                     ATRVSTGEECAIIIEDGYSVENKAHSFKTAEGGSFAETVAPTWQGGF"
     gene            6982..8055
                     /locus_tag="VBE34_000129"
     CDS             6982..8055
                     /locus_tag="VBE34_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7262888.1"
                     /translation="MQITRGKRARAQRVIIYGPEGIGKSSFAAQFPEPLFIDTEGSTD
                     NMDVARMDKPTSYTMLKNQIAFVKANPTCCKTLVIDTIDWAESLIVDDVCAQHGKKGI
                     EDFGWGNGYTYTKEEVGRFLNMLQELIELGINVVLTAHAQMRKFEQPDEMGAYDRWEL
                     KLGKKTSSQTAPLVKEWADMVLFANYKTVVMTADNNKKKATGGTRVLYTQHHPAWDAK
                     NRHGLQEEMPLDYAGIAHIFAQAPSQPVAETPPVQQPVPTQPIHEPVHEQQAQMSGPT
                     AATEYPSVLPKALTDLMTPEQVTPDELVAVANIRGHFPPLTPIENFPPDYWNMIVANW
                     GATLEVIKTQVRATEQPFTVEGS"
     gene            8151..8756
                     /locus_tag="VBE34_000130"
     CDS             8151..8756
                     /locus_tag="VBE34_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262889.1"
                     /translation="MTEYNNNFEREFGWDDTIQEDAKEFITLQPGDYVFTVTDFERAR
                     HTPNPQKTGKLPACNKAVISLEIETDEGVASLKHNLFLHSSTEGMLSAFFGAIGQKKH
                     GEPLKMNWNTVVGATGVCSVKNRTYNDNVYNEVKSMIYADSVDWTKVLNANTQPQAPQ
                     PTYQQPAQQTTNFAPQQQPAPQSYQQGQMQASQQQGGWGGF"
     gene            8756..9364
                     /locus_tag="VBE34_000131"
     CDS             8756..9364
                     /locus_tag="VBE34_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262890.1"
                     /translation="MGKAKDYTGQRFGRLVVIERLPQDPYTKAKYKCQCDCGKLTIVN
                     SSNLATGHSTSCGCIVTKHGFARKERLYNIWVGMRQRCRDKNSNDYIKYGGRGITICD
                     EWEEYIQFKQWASSNGYRDNLSIDRIDVNGNYEPSNCRWANSTEQANNQRNNHLITFK
                     GKTQTIKQWSSELGISNITLLTRLNRGWSITKALTTPTQKGV"
     gene            9370..10953
                     /locus_tag="VBE34_000132"
     CDS             9370..10953
                     /locus_tag="VBE34_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEO7262891.1"
                     /translation="MKLREYQEEARSAVQQEWEQGKKKTLLVLPTGCGKTIVFSKIIE
                     DRVKLGERVLVLAHRSELLEQASDKLMTATGLGTALEKAESTSLGSWYRVVVGSVQTL
                     QREKRLSKFPTDYFDTIVVDEAHHAISDGYQRVLQHFDSSNVLGVTATPDRGDKQNLG
                     KYFDSLAYEYSLVDAIKSGYLSKITAVTIPLTLDLSTVSQQAGDFKASEIGTALDPYL
                     EQIADEMVKQCSNRKTVVFLPLVKTSQKFRDILNQKGFKAAEVNGESKDRAEVLEDFD
                     NDKYNVLCNSMLLTEGWDCPTVDCVVVLRPTKVRALYSQMVGRGTRLAPGKEKLLLLD
                     FLWHTERHELCRPAHLIAKTPEVAQKMVENMTDETEVAFELLEAEEVASKDVVAEREE
                     ALAKQLAEMRKRKRALVDPLQFEMSINAEDLVDYVPEFGWEMAPASEKQLKTLEKFGI
                     FTDEIGNAGKAAKLLDRLDKRRQSGLTTPKQIRMLERYGFKNVGMWKFEVASNMINRI
                     AASGWRLPKGINAREYQPE"
     gene            10962..11159
                     /locus_tag="VBE34_000133"
     CDS             10962..11159
                     /locus_tag="VBE34_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262892.1"
                     /translation="MENINEQIEKFISNFADEAIEKSETYSEAILYVDKHSSLTEFGQ
                     VVKKAIQEKIRDIALNSRIIK"
     gene            complement(11152..11403)
                     /locus_tag="VBE34_000134"
     CDS             complement(11152..11403)
                     /locus_tag="VBE34_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262893.1"
                     /translation="MPEIKITFSDGSIVIFHEEQSFQSVVKSNDAMSLSKIYSLWNHV
                     HDGLIPSFLELIANSQFFFDLENPQIYYASNAVIKIEAI"
     gene            11474..13753
                     /locus_tag="VBE34_000135"
     CDS             11474..13753
                     /locus_tag="VBE34_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO7262894.1"
                     /translation="MAENDFNLLPLLDYINPATVDYQTWVQVGMALKHEGYTAMDWDV
                     WSQSDSRYKKGECFAKWDSFQGNGFGTITGATITQLAKDNGWTSSEYRNSDDAHELSW
                     DDTIDRDYKIVDKNWIESKEIQEPRNWNPVQDLITYIDTLFESTDKVGYVTETYPITL
                     DTGEIVYKPTKGAYDRTAGQLIESLQKNPTDLGAVFGDFKEEAGAWIRFNPLDGNGVK
                     NDNVTDFRYALVESDSMELGKQYALFKELELPIATLVHSGKKSLHAIVKVDARDYQEY
                     RKRVDYIYQICKKNGLDIDTQNRNPSRLSRMPGVTRNGHKQFLIDTNVGKTNYEEWYQ
                     WIEDLNDDLPDPETLADEWDNMPELAPELIKGVLRQGHKMLIAGPSKAGKSFALIELS
                     IALAEGKEWLGWQCEQGKVLYVNLELDRPSALHRFRDVYEAMSLPPANIKNIDIWNLR
                     GKTVPMDKLAPKLIRRSLKKNYQAVIIDPIYKVLTGDENSADQMAHFTNQFDKVATEL
                     GCSVIYCHHHSKGSQGGKKSMDRASGSGVFARDPDALIDLVELELTEELIKSRSEKAA
                     AKIYQQALQEKALAYYQQEVTLDDLESRYQMQQHFDKAIKDIMIKQPYLEAVKKAQYE
                     VEISTAWRVEGTLREFAKFQPVNMWFSYPVHDVDTTGVLADISLEDNVPTWKKNFEKK
                     SPRESREKKSQKVETAINSLNDGIEPVTIDNLIEYFSTEDKPVSEKTIRRWIKENGKF
                     EVKNKQILPIEEPKTNKSL"
     gene            14123..14320
                     /locus_tag="VBE34_000136"
     CDS             14123..14320
                     /locus_tag="VBE34_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262895.1"
                     /translation="MKEYKKRMIVKYQELKSRAEKLSVLINKFYIDSLDFKLQCPIEL
                     LVAQWHAMGAYLKILEQRAAI"
     gene            14347..14745
                     /locus_tag="VBE34_000137"
     CDS             14347..14745
                     /locus_tag="VBE34_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEO7262896.1"
                     /translation="MTIEFFIPMKKIPTTTHQQKKVRVVNGKPQFYEPDSLKEARAMF
                     MDKFAQHVPKEKLNGPIRLTTKWLFPKIKKSVNGQYKDTKPDTDNLNKLPKDCMTELG
                     FWNDDAQVASEIIEKFWADTVGIYVKVEEL"
     gene            14742..14957
                     /locus_tag="VBE34_000138"
     CDS             14742..14957
                     /locus_tag="VBE34_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262897.1"
                     /translation="MKIDYIDFFERVVPGWMRESNKKMQELGFGTEQYWVWVNMSIVD
                     ICESYGNDDLVNGQFHMIWEWLERKTK"
     gene            14954..15190
                     /locus_tag="VBE34_000139"
     CDS             14954..15190
                     /locus_tag="VBE34_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEO7262898.1"
                     /translation="MTGELVHKPKHYNHGEIDLIESWYKTYPWDQFVAIMESHIDKYI
                     KRHRYKGNATQDLEKALEYTKRLVEYWLLESVNE"
     gene            15187..15417
                     /locus_tag="VBE34_000140"
     CDS             15187..15417
                     /locus_tag="VBE34_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262899.1"
                     /translation="MNEQNNKLHKQSIVIVVLTVAVSVLLTTNIALYNYYQPQITGLQ
                     QQLIRTQYRLKESSEQNQRQTKRIAELTGNGG"
     gene            15420..15752
                     /locus_tag="VBE34_000141"
     CDS             15420..15752
                     /locus_tag="VBE34_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262900.1"
                     /translation="MSLIDEVKECGSESHKKWFDRYFDGLKIEEKLKQSAMKGFSGYK
                     ISIPKNDEYLARRLDSQKTIDLLKYRLGDGFDVEIKNLESSYKIFGKPVVLSRDLFIF
                     WRTGDKNG"
     gene            15830..16243
                     /locus_tag="VBE34_000142"
     CDS             15830..16243
                     /locus_tag="VBE34_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO7262901.1"
                     /translation="MSKLSNAQLKAFDEWLFDYRDIERKIAIRKLELQTPVSTDINVG
                     GGKSNLVAKETEDIVIKWSKDGKIKSYENFRESVDRVKALLDDELNYIFDLRWGTGSN
                     NTWEEIAYKIHTSRAGTYRKRDRILTLFAQQIGKL"
     gene            16364..16795
                     /locus_tag="VBE34_000143"
     CDS             16364..16795
                     /locus_tag="VBE34_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEO7262902.1"
                     /translation="MSKLTLKQKRFADEYIISANATAAAIKAGYSKKTARSIGQENLT
                     KPDIKAYIDERIEKLESAKIATQEEVLQYLTSIMRGDQQEKTLISIGELGQEIVDIDV
                     GAKDRIKAAELLGKRYRLFTDKIEMDISSDVTISVGEWDDD"
     gene            16785..18065
                     /locus_tag="VBE34_000144"
     CDS             16785..18065
                     /locus_tag="VBE34_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEO7262903.1"
                     /translation="MTTKQRPKINIVIKYPSKVFNKHIYDKLYNYDNFTEVHYGGASS
                     GKSHGVFQKIILKALNPKFKHPRKILVLRKVGATVRDSVFADIMSNLSYFGVLDKCKI
                     NMSAFRITLPNGSEFIFKGMDNPEKIKSIKGISDVVMEEASEFTLDDYTQLTLRLRDK
                     KHLEKQVYLMFNPVSKANWVYKAFFIKSPKNTVVYQTTYKDNRFLDDVTRENIEELAN
                     RNEAYYKIYALGQFATLEKLIFPKYEKRILNKDKLSHLPSFFGLDYGFINDPSAFLHV
                     KIDDANKKLYILEEYVRKNLTNDKIANAIKSLGYAKEEIRGDSAEKKSNQELRNLGIQ
                     RMIDVKKGAGSVMQGIQYMLQYDIVVDERCVKTIEELENYTWKKDKKTNEYINEPVDS
                     YNHCIDAIRYAVQDRIYQSSDRNKRLNNAKYYFR"
     gene            18080..19609
                     /locus_tag="VBE34_000145"
                     /pseudo
     CDS             18080..19609
                     /locus_tag="VBE34_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            19569..21017
                     /locus_tag="VBE34_000146"
     CDS             19569..21017
                     /locus_tag="VBE34_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEO7262904.1"
                     /translation="MLMDKKNQQQDHLDYWQKRQEDIVSYLDRADLDIFNELSRVYSN
                     QSLSLQKDLYDFYNRFAEDNKITYQDAVKKLRHEDLSDYAANANRYREQAEKDPELLK
                     RINEQYVSAQATRLDMLNLELIHRAGTMRGILDGLFSDHLRKAASYAYQKSVGGRSGT
                     INGPALEQLIKTPFDGYNYSEQLWGNVDNLVKDLQEAFKKGFVRGLGPREMARELGKR
                     YDVARHRAETLIRTDGTMIINNATAKRYIDAGIKYYRDLVQLDDRTTQICRSKARENK
                     KKLLSEIQPGINAAPYHFNCRTTIIPDEDELNTDVEPIADKSTKYFKDVTSDWIDGQE
                     YESQISMLNEYTKGGGTYRVDGHNVVLDHSNYEYRVANWLSKKTGLQVEMVPRVNFPE
                     NINTPDYLVDDVPFDLKEITGSGKNVIDGNLRKAKKQAPNIIFDITKTPLSFEEILGQ
                     LEHIYKIDRRGLNISIIKNKDEVLAVVEKERR"
     gene            21045..21233
                     /locus_tag="VBE34_000147"
     CDS             21045..21233
                     /locus_tag="VBE34_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262905.1"
                     /translation="MGVRPPSFTTISIHQYSRIIKEEDMFIWRMVLVALGVLVLILII
                     GIAAIAVKSIIAEVKKEG"
     gene            21238..21441
                     /locus_tag="VBE34_000148"
     CDS             21238..21441
                     /locus_tag="VBE34_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262906.1"
                     /translation="MNKRIKKKRKLERAIVMLIAENAMQAEALRNQHKRIEQLEQIVE
                     HNAQATNNELDYIKNNYKKKWKK"
     gene            21584..22153
                     /locus_tag="VBE34_000149"
     CDS             21584..22153
                     /locus_tag="VBE34_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEO7262907.1"
                     /translation="MAEENVATETTEQVDTQKEAVEQAKQEHERTFTRADISKMMTAE
                     RAKWEAEQSDAIEKARTEGERLAKMSKDERAKEEEQKRLDAIAEREKAVAEREMRIET
                     QTLLVEKGLPLEFIDVVLGTTAEEVKTNIDNLQIIFDKAVEKRVNNRLTQKPPRTGNG
                     SVGMTKAEIMAVEDDDERMRLIAENRNLF"
     gene            22172..23068
                     /locus_tag="VBE34_000150"
     CDS             22172..23068
                     /locus_tag="VBE34_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEO7262908.1"
                     /translation="MAEKNLNTMADLGDIKSIDFVNKFSKNINDLLKLLGVTRREELT
                     SDLKIQTYKWEVTLDQTDPGEGETIPLSKVTRTKDKDYTVKWFKKRRATTAEAIARHG
                     AARAITEADKRIMRELQNGIKDAFFTFLKTKPTKVKGAGLQKALSLSWAKLATFNEFE
                     GSQLVSFVSPLDVANYLGDTKVGADASNVFGMTLLKNFLGMQNVIVMPSVPEGKVYST
                     AVDNLVFASLNVRGGDLGGLFADYTDETGLIAAARQRQLSNLTYESVFFGANVLFAEI
                     PEGVVEATIEADKEVPGAVPGG"
     gene            23074..23430
                     /locus_tag="VBE34_000151"
     CDS             23074..23430
                     /locus_tag="VBE34_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEO7262909.1"
                     /translation="MDKELLLVEVKIFKGIAKNDASQDELIKLAIDESIERILAKLNE
                     YSDEELLEIPRRLTFIARDVAIKRYNRLNSEGAKADSEEGRSFSWEDYLTEYDSILRN
                     VAYGKRCRGKGVARFI"
     gene            23441..23719
                     /locus_tag="VBE34_000152"
     CDS             23441..23719
                     /locus_tag="VBE34_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262910.1"
                     /translation="MIYKDRVILVYVDEQDDFLDKRTVEKPSGKIPCMENTFTKSEQM
                     GLFGKYDLNAFKLHLQGHYDGFSKIIYKGKPRLIKGLAHHKNSTVIYV"
     gene            23716..24060
                     /locus_tag="VBE34_000153"
     CDS             23716..24060
                     /locus_tag="VBE34_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO7262911.1"
                     /translation="MSLTYRMRGLDRFLRSVERKQKSARIAVDKKLSKSAARIERQAK
                     ILAPVDTTWLRSQIYNEQQKLLHYRVVSPVLYSIYLELGTRKMEAQPFLDPALRKEWP
                     VLMANLKKMFKR"
     gene            24064..24423
                     /locus_tag="VBE34_000154"
     CDS             24064..24423
                     /locus_tag="VBE34_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262912.1"
                     /translation="MIYSPSTLYLNEVKNRLGTLNIPIYFKLPKSDVLEPFIVIGSNV
                     SDTSKTAQTGAVVGDFGLNIDAFLPATSRTDAEEVKSRMIMSLGRNKRINAQLLMDNS
                     VGREVYHIAINITEILI"
     gene            24435..25067
                     /locus_tag="VBE34_000155"
     CDS             24435..25067
                     /locus_tag="VBE34_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEO7262913.1"
                     /translation="METTVEITTAKPIKGKNIFYFIQSVDAELGSKAILPAYRTDGTT
                     TLGGDFIDEQTQQGRVLEKATDEHSIDLTSYYTPKDKAIEIVEIAKTTGKSVKVWRVI
                     ADESLKEKGSGTLKDREVYPAKFGYGKIDEIEYSEGVEDLVEASYALNIIDSLKDGKF
                     PLTAEDIALLTSVYAYQNPGETTGDYDNIQKKEVSGSSPGAVPEKLSEGH"
     gene            25118..25573
                     /locus_tag="VBE34_000156"
     CDS             25118..25573
                     /locus_tag="VBE34_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262914.1"
                     /translation="MELTVAKRSIEIKFDFKTMFKINNRLGTVNKETGERNADGVGTL
                     FYKILERDDSAVVDLVKLSVGSGKKALSEDEVLDSIAELVEEEGSTEALFKEIENDMV
                     ESGFFKEKISKYIESMEKSVKYLEAREDADEAQIQIVKDMIGKMKDAIS"
     gene            25648..25878
                     /locus_tag="VBE34_000157"
     CDS             25648..25878
                     /locus_tag="VBE34_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262915.1"
                     /translation="MEGLQYKQIAERENLSELALNLRYTLNAKNVNANKLKLDKQRNK
                     IKRTYQNKQDLTNISNDFAERVAKLNLHFQNL"
     gene            25907..30133
                     /locus_tag="VBE34_000158"
     CDS             25907..30133
                     /locus_tag="VBE34_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEO7262916.1"
                     /translation="MAGTFDGAIFADIGANTKDYEAAMARIVSTTQNAFRKAQDAAVN
                     NSNKMIQMIGQIMAQLANNGQSLGQRLGNAYSTGLKLSLGQIQRIAASIGEKIPEPIK
                     NGFNKAFSIIQNSVQKLSSAIPQPIKSAFTSATSAVVSFSSKITSAVSAAFNSVSSKA
                     STVADKISSSFGGRITSAVTNLATKLSTGLSNSFSRMSSSATSALNGISQRFASASSA
                     GEKLRSTVGSIISAFSLMAIAQKGIQAITGAMDGAISRVDTMNRFPKTMALFGYSAQQ
                     SKASIDKLSKGIEGLPTPLDSAVKSAQQLAITTGSLDKGTELALAFNNAMIGYGATTE
                     GAEQALRQFNQSLGSGKIYAEEFNSVSEAAPGLMSKMAESFGFGKNGVQDLKAALSDG
                     SITAQEFADKMIELNKAQGGFAEMAQSSAGGIRTAWKNVHTAVVKGVAGMISAFDEAA
                     KANGMKTIAESINSIKPAINSAFATVNSIMPNLVAAFARISKMVNIDFSPLTSGIKSA
                     FGLLNTALGEFAKTGEVSSATIDRIKSKIASIGPTAIAAWALLNPASAIATIMPLISI
                     IGKIGVALGTVGSALGSFGAMVASGLSTASGIIGAFAATVSGLPAIFATAAARGVAIL
                     GAMTQAMGAVVSVAMAAIGPAAILGLVVAGLGLVNNQFGTQIDKLLNIAITRGSQIIT
                     NLVNGITSQIPQLIASGAELISKFANAITVLLPVIIQAGVQLISSLVQGVGQNATSLI
                     SSAIQIIGTFISSIASALPQLLAVGMELIVNLVQGIVQNIPLIIQYAQQIINNFGASL
                     QANMPSIVNNGIAILTNLVQGIIQMLPAVVQIATQVITGFISGIAQYLPQVIQGGIQI
                     IIMLVQGIIQNLPQVIQAAMQIIQSLISGLSQALPQIISAGLQLVVQLAVAIIKGLPQ
                     IISAGIQLIIGLGKAMLEAIPNALSGVWEGIKGGFSSMWDQITGKSSTSAAKVTSDMS
                     TMTMNVGMQTTTMANQTNIDTTTMLNSISQNTGLANLNATTNATQMATNVNAQTASMA
                     NQGLLNAAGFNEALSSNMTLANTNATNQANQMTANVNNATSQMSLGSLPNFLELSQQA
                     TSNMQTAQANATTSANGMSSGVTGATAGMASGVANNMSGITSTVSTGMNQAANAAKSG
                     GANISNSVNSSFTQAKSTVSSSMSGMNSTVTSGMNSINNTVKSGGNNIKSTFTSSFNS
                     AKSTVSNAMSSIRSSISSGMNSAVSVTRSAMNSVVSAMNAAASGARSAGYNTGIGFAN
                     GLASTAGTIMAIANSIAANVAATIRSAMDIHSPSRVTRELGAYTGEGFALGMQDWMDD
                     INVIGKEYANAVTDQRWETDSQLATSANISSAGVSSSLDNLSDEVRNQQLTSPTFEVH
                     NELVGDEIYTTIKSKEARESNKDNYFNFAT"
     gene            30146..30988
                     /locus_tag="VBE34_000159"
     CDS             30146..30988
                     /locus_tag="VBE34_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEO7262917.1"
                     /translation="MDLLVQKSDQSFRFSEIGLRILDVDDRSSSLEVDSRTVKGRSGK
                     IFASAKYGTKKVKVNGRLVVASIKDFMTKKDDVNGLLVDSEPFYITKMYPQKENLYDF
                     ELPGMKAGDLDLLNQSHTAWHYRWKVYVSSEPEYTFVGKSSEGLKYNFTIAFETAELP
                     FGETIAQNIVITNGTFNYSGTAPFSQLEYPWSVELTSTGGQSSFYLEIDGRRWAYQYS
                     APLNQGDVIVITGISTTLNKINVTARTNHEYFVLKQKVNKTILYSTNFQGTIKFLNFK
                     ELYK"
     gene            31001..34828
                     /locus_tag="VBE34_000160"
     CDS             31001..34828
                     /locus_tag="VBE34_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO7262918.1"
                     /translation="MTTFLDHKDNEFEAQAVVKTTNAVNGERSLSGTIYTNEEVLNKV
                     DKGWKLEFDDEYYKIIYAKPTDTGNKIQVEFDAVHQFFYDFDKSSLHKQLNDGSHTFQ
                     AYLNFIFNGSGYTYSLEVVVKSFEKQSFGYKSRLDLFNDIIKSAGVEFFVRGKVVRIL
                     QKTGTDLSTIVRKNFNMNEIIIEKKINDFITYQKGFGAWTDQEDHSKGRLEVEYESPL
                     AKEYGRIEGEPVVDERYTNVDNLKSVLKQNVDSSYNIAVKIDIEDLTKAGYQYTQPIA
                     GDYIMAINETLGFKEKIRIVSFESEYDVTGQLINHKVTCNDIGTIKKQTVAVSQLSSK
                     INNNQEILDNAVFKANQALASADGKNTNYYGTEMPVDDPKGTLRKGDLLFLTVGDTTR
                     MYFWNGAEWIINSFSDDIDFVKKEITNKIIEVNAAMKLADEQLTEKYNAIVAKNVSQD
                     ELINQAKNLSDSAKKDATEALTNLISESKKLTDKMSALSKTEVEHYSTTNLQLTQLDG
                     TVKGLQTSYEALLKKDGDITQTLANYKQTIDQNSTSITANKKTVDGTLSSLQTQVTQT
                     ANEITTRLSQTNIEALITSKSAVIADNKVKETADSFSREITRVNNTIDNLKIGTVNLL
                     SGTKNWTKNWFNRNNWQSDDDNLTIGNFSLTVLKRKAMWSGISQLYAVKSGENYVFSA
                     YAKSSIDNDVVNLYLLPNDQSQQAKISISFKSITLNTDWQRIVIKFNVTSDGYILPRF
                     ERFNENGWLYIAGYQLEHGNVTSDYSESPADQEDKMTVEFNKINDTVNSHSQLIGKQN
                     ESLTATIQKVDNIQSTVSNVDGRLSRVTQTADGIVATVSQLDNNILPGTKKFTGWQTE
                     GAVLEDLTQSPYPFVFKKWVSGNKVSPLIEYDVKKDQEYTFTAYISREQAGNLYFYLY
                     DLWNHHITSTTPRETIIKDVTQTIRRFKITFIPTRDGKIRPRFAMLASDPGWFMVGGF
                     MLSKGTADLPWSESKQDIKDNVTAINTIVAQTANSWAIKNLTSNGTVLSQLNLTDGNV
                     KLEGKLIHLSGQSIIDNGIITNAMIKDLTADKITAGTIDANRINVINLDASNITSGRM
                     SADLIRSGVLISQNGAMLTDLNTGQIEFYTDNPAIKRITAGYPNQFVKFATGNVEGKG
                     AAGVTVIGSNRWGSESSNDGGFVGIRAWNGKDIDTLDLVGDSIHLASSAYTNADGWEI
                     ITLPNQLSIDARNINHRVTSKIKVGDIWLWKNTSTYTSMKDTINMIIDNLQLLHNNKT
                     TEKGYSYTIPGKI"
     gene            34837..34989
                     /locus_tag="VBE34_000161"
     CDS             34837..34989
                     /locus_tag="VBE34_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262919.1"
                     /translation="MEEKIKSNLALQIAQLSLDKATLQAQNEQLQLQIAELNQQLDEI
                     TKPGAK"
     gene            35000..35416
                     /locus_tag="VBE34_000162"
     CDS             35000..35416
                     /locus_tag="VBE34_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEO7262920.1"
                     /translation="MKQWNIVGKPFPIYQDGVVVKTDVRLQGDDGTYLPQELIGNHVD
                     KDNQSLIRLVLETFAKENVVDFAMLESVKDIEQLKADKVTLIKKIAEADEAISKMSQA
                     TKDMQLKVDKAVVELTDLVTSSVSSLSVPSTSMESS"
     gene            35479..35640
                     /locus_tag="VBE34_000163"
     CDS             35479..35640
                     /locus_tag="VBE34_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262921.1"
                     /translation="MTYTFKKDHLIVDVWYRRVANGICDFEAVPKLFNLRDVVLELLS
                     QKVTDKESE"
     gene            35650..35946
                     /locus_tag="VBE34_000164"
     CDS             35650..35946
                     /locus_tag="VBE34_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262922.1"
                     /translation="MIDFVQVGAFAGAALSILGVWGFIVNPFKKAMEANEIAMAQLKD
                     SLKELAYELKNIDRDREITKAVIDRHEERLGRVEDDIIINKEQIKTLFNNNKEK"
     gene            35948..36286
                     /locus_tag="VBE34_000165"
     CDS             35948..36286
                     /locus_tag="VBE34_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO7262923.1"
                     /translation="MNEFTTQIITVSVPIFGIIAGILTHEVKKLLIKKGGEKAVKIAE
                     IVARNAVEAVEQISAEVGGIKGQDKLTEAKTAVIDGLSQYNINLTQTQLETFIEAAVK
                     RMNDEWKKGQ"
     gene            36288..37007
                     /locus_tag="VBE34_000166"
     CDS             36288..37007
                     /locus_tag="VBE34_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7262924.1"
                     /translation="MVINIEQAIAWMASRKGKVTYSMDYRNGPSSYDCSSSVYFALRS
                     AGASDNGWAVNTEYEHDWLIKNGYVLIAENTNWNAQRGDIFIWGKRGASAGAFGHTGM
                     FVDPDNIIHCNYGYNSITVNNHDEIWGYNGQPYVYAYRYSGKQSNAKVDNKSVVSKFE
                     KELDVNTPLSNSNMPYYEATISEDYYVESKPDVNSTDKELLVAGTRVRVYEKVKGWAR
                     IGAPQSNQWVEDAYLIDATDM"
     gene            37346..38305
                     /locus_tag="VBE34_000167"
     CDS             37346..38305
                     /locus_tag="VBE34_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEO7262925.1"
                     /translation="MAENFQHKSYREQVELLESRGIVFSGRKAKSKAEYSLSVISYYK
                     IKEFAKPFAKIQKDGQKKKIDYQGTKFEIVISRYYQDKNLRLNLLHAIEDIEVAIKTK
                     IAYVLGKNGLGSYGYLDFSKWCNKEEYCKHYLSYSENNFKKQLKRELRKASSSELDEK
                     LKLDRQKYPPIWLAVNMLTFGQMVNLLELMSTTNLTQISSTFSCSNPELISWLKCINL
                     ARNICAHNSNIIDFKFITVSKLKEEWKDFLFEYKEGVFSNRIALPFLIVLEMMGKINP
                     KYHFRDIIDSLHKLIKDEDTAKYYGFASMNTIKEIKKKKNFRG"
     gene            38460..38660
                     /locus_tag="VBE34_000168"
     CDS             38460..38660
                     /locus_tag="VBE34_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO7262926.1"
                     /translation="MSQEKLKAKVEQASGSLKEGAGKLTGDKELEAKGFVEKTIAKGK
                     ELADDAKDAVEEAVDAVKEKLK"
     gene            complement(38701..38871)
                     /locus_tag="VBE34_000169"
     CDS             complement(38701..38871)
                     /locus_tag="VBE34_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262927.1"
                     /translation="MDKELTPQEKANKKWAENNREHRTYLSKRSTARSFINKNATKED
                     LLELKQLIESKL"
     gene            39291..39470
                     /locus_tag="VBE34_000170"
     CDS             39291..39470
                     /locus_tag="VBE34_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000965654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO7262928.1"
                     /translation="MLYIDEFKEAIEKGYISSDTVMVVRKNGKIFDYVLPHEKVREEE
                     VVTVERVEDVMRELE"
     gene            39876..40073
                     /locus_tag="VBE34_000171"
     CDS             39876..40073
                     /locus_tag="VBE34_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262929.1"
                     /translation="MSEKAILLIILIAFVIIAIIKIALLKLYMNTIKPKVDNEGNIIT
                     KPVKDDLTPEEREEREFDKDW"
     gene            complement(40117..41049)
                     /locus_tag="VBE34_000172"
     CDS             complement(40117..41049)
                     /locus_tag="VBE34_000172"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEO7262930.1"
                     /translation="MVSLKTEIAGFSFDNCLMNAAGIYCMTKEELLAIENSEAGSFVT
                     KTGTLKAREGNPQPRYADTDWGSINSMGLPNKGIDYYLDFVTELQDQDNSKNHVLSLV
                     GLSPEETHIILKKVENSSYNGLIELNLSCPNVPGKPQIAYDFEMTDLILSEIFSYYQK
                     PLGIKLPPYFDIFHFDQAATIFNKYPLAFINCVNSIGNGLVIDDETVVIKPKNGFGGI
                     GGDFIKPTALANVHAFYKRLNPSIKIIGTGGVKNGRDAFEHILCGASMVQIGTALQKE
                     GPEIFQRVSRELKEIMADKGYQSLEDFRGQLNYL"
     gene            complement(41237..42472)
                     /locus_tag="VBE34_000173"
     CDS             complement(41237..42472)
                     /locus_tag="VBE34_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7262931.1"
                     /translation="MALKELTAKEFESYSGNYDLQSFMQTPEMAKLLKKRGYDITYMG
                     YQIDGKMEIISIVYTIPMTGGLHMEVNSGPAHSNSKYLKHFYKELQNYAKSQGALELL
                     IKPYDTYQEFTGEGEPKGAPNTYLIDGLTSIGYHHDGLHIGYPGGEPDWHYVKNLEGI
                     TPQNLLKSFSKKGRPLVKKAMSFGIKIRVLKREELHIFKDITSSTSDRRDYMDKSLDY
                     YQDFYDSFGDKAEFVIATLNFREYDHNLQLNAKKLEEQITVLDNRHQNNTDSAKYHRQ
                     RTELVNQLASLDKRRKEVEPFIQKFGNQDVVLAGSLFIYSPKETVYLFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRQSTFYNFLGIQGNFDGSDGVLRFKQNFNGYIVRKMGTFRYY
                     PNPLKYKSIQLLKKILRRT"
     gene            complement(42491..43702)
                     /locus_tag="VBE34_000174"
     CDS             complement(42491..43702)
                     /locus_tag="VBE34_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7262932.1"
                     /translation="MYREITAVEHDRFVSESNQTNLLQSSNWPKVKDNWGSQLLGFFD
                     GETQIASASILIKSLPLGFSMLYIPRGPIMDYSNLDIVTKVLKDLKAFGKKQRALFIK
                     CDPLIYLKMVNAKDFENSPDEKEGLIAIDHLQRAGADWTGRTTDLAHTIQPRFQANLY
                     ANQFGLDKMSKKTRQAIRTSKNKGVDIQFGSHELLEDFAELMKKTEDRKGINLRGIDY
                     YQKLLDTYPNNSYITMASLDVAKRLEKIEKECQIAQSERIKSLELNREKKVKQHQDTI
                     DRLNKEIDFLKEAQKAYDRDIVPLAATLTLEFGNTSENIYAGMDDYFKSYSAPIYTWF
                     ETAQRAFERGNIWQNMGGIENDLSGGLYHFKSKFEPIIEEFIGEFNIPVNRLLYKASN
                     YVYALRKKRNS"
     gene            complement(43715..44950)
                     /locus_tag="VBE34_000175"
     CDS             complement(43715..44950)
                     /locus_tag="VBE34_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7262933.1"
                     /translation="MGNKLMYHVTVGISEKEYDAFAIASSQTNLLHSSKWAQVKSNWQ
                     NERLGFYKDDQLVAVASILIKSLPLGFTMLYIPRGPIIDYSNKELVGFVLKTLKNFGR
                     KKRAVFAKFDPALLLRQYHLKEENVAEEIDESRQAIDNLKSAGAQWIGPTKAISETIQ
                     PRFQANIYTKANIEENFPKHTKRLIKDAKHRGVQIYRANIDDLPKFATVVALTENRKG
                     VALRNENYFHQLMTIYGEDAYLYLAKVNLPKRLAQFKEQLLQIQRDLSETPSHQKSRL
                     TRLNQQEASVKQYILEFQEFSKKYPDEPVIAGILSIRFGNVLEMLYAGMDDSFRKFYP
                     QYLLNARVFEDAFKNGIVSANLGGVEGSLNDGLTKFKSNFNPMFEEYIGEFNLAINPL
                     LYKLANLAYTIRKKQRHSH"
     gene            complement(44935..45747)
                     /gene="yidA"
                     /locus_tag="VBE34_000176"
     CDS             complement(44935..45747)
                     /gene="yidA"
                     /locus_tag="VBE34_000176"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEO7262934.1"
                     /translation="MTSIKLVAVDIDGTLLNSKREITPEVAKAVQEAKSKDVKIVIAT
                     GRPIIGVQDLLEELKLNEEGDYVITFNGGLVQDTATGDDIIKETLTYEDYLDFELLAR
                     KLGVHMHAITKEGIYTANRDIGKYTIHEATLVNMPLFYRTPEEMGDKEIIKLMMIDQP
                     DILDAAIAKIPKKVLDNYTIVKSTPFYLEILPKNVNKGTALLHLAEKMGLTVDQTMAI
                     GDEENDRAMLEVVGNPVVMQNGNPELKKIAKYITKSNEESGVAYALREWVIN"
     gene            complement(45819..47135)
                     /locus_tag="VBE34_000177"
     CDS             complement(45819..47135)
                     /locus_tag="VBE34_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO7262935.1"
                     /translation="MNEKVFRDPVHTYIHVNNQVIYDLINTKEFQRLRRIKQTSTTSF
                     TFHGAEHSRFSHCLGVYELARKVTEIFDEHYSDLWNKNESLLTMAAALLHDIGHGAYS
                     HTFERLFNTDHEAYTQEIITNPTTEINAILRKVAPDFPDKVASVINHSYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYYTAASYGQFDLTRILRVIRPTDSGIAFARNGMHAVEDYIVS
                     RFQMYMQVYFHPASRAMELLLQNLLKRARFLFDTHRDFFEQTSPNLIPFFTDQYDLQD
                     YLALDDGVMNTYFQSWMQADDNILADLANRFINRKVFKSITFEEADKENLVKMKELVS
                     QVGFDPDYYTGVHANFDLPYDVYRPEHSNPRTEIQIIQKNGQLAELSSLSPIVKALTG
                     SNYGDQRFYFPKEMLTLDSLFSSTKEEFQSYITNEHLTLTKDNSHS"
     gene            47199..47597
                     /locus_tag="VBE34_000178"
     CDS             47199..47597
                     /locus_tag="VBE34_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEO7262936.1"
                     /translation="MREVMKLEINNNIQIDNETEMIHEIHDCQFIEKGSYVYLNYINA
                     EGERVVIKANHEELLMTRFSNPKSVMRFHRETPALVNIPTPLGIQHLITETSHYQFDL
                     SQQRLHINYVLKQTETGACFANYELKIQWY"
     gene            47941..50625
                     /locus_tag="VBE34_000179"
     CDS             47941..50625
                     /locus_tag="VBE34_000179"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HEO7262937.1"
                     /translation="MLKEQKKSLFYTQGQEEVLTSLESSREGLSTTEAKNRLEMYGRN
                     ELEEGKKRSLIAKFFDQFKDLMIIILLVAAALSVITEGMHGLTDALIILAVVILNAAF
                     GVYQEGQAEAAIEALKDMSSPIARVRRDGHTIEVDSKELVPGDLVMLEAGDVVPADLR
                     LLEAASLKIEEAALTGESVPVEKDISQVVAEDAGIGDRVNMAYQNSNVTYGRGYGVVT
                     NTGMYTEVGKIADMLANADESETPLKQSLVQLSKLLTYLIVIIAVITFLVGIFVRKEG
                     WIEGLMTSVALAVAAIPEGLPAIVTIVLSMGTKTLAKRNSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGVLQSSSEEISVDNNTLRIMNFSNDTKIDPSGKLIGDP
                     TETALVQFGLDKNFDVREVLKNEPRVAELPFDSDRKLMSTIHKESDGRYFIAVKGAPD
                     QLLKRVTKIEDNGLVRDITAEDKEAILNTNKELAKQALRVLMMAYKYETQIPTLETDI
                     VESDLVFSGLVGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGIIDA
                     NDTEDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNDGKVVAMTGD
                     GVNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKS
                     IQYLLSANMAEVFTIFFATLLGWDVLAPVHLLWINLVTDTLPAIALGVEPAEPGVMTH
                     KPRGRQSNFFDGGVMGAIIYQGILQTILVLGVYGWALMYPEHAGYRMIHADALTMAFA
                     TLGLIQLVHAFNVKSVYQSIFTVGAFKNRTFNWSIPVAFILLMVTIVVPGFNKLFHVT
                     HLSSTQWLTVVIGSLLMVVLTEIVKFIQRKLGQDEKAI"
     gene            complement(50670..51530)
                     /locus_tag="VBE34_000180"
                     /pseudo
     CDS             complement(50670..51530)
                     /locus_tag="VBE34_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163483.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            51682..53613
                     /locus_tag="VBE34_000181"
     CDS             51682..53613
                     /locus_tag="VBE34_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEO7262938.1"
                     /translation="MSNFYKLLKEKFPRKEDIVTEMINLEAICQLPKGTEYFISDLHG
                     EYDAVDYLLRTGAGSIRAKLLDCFDWQKIVAVDLDDFCILLYYPKEKLAFDKMNLSVS
                     AYKTKLWEMIPLQIQVLKYFSSKYTKSKVRKQLPGKFAYIIEELLAEIDRNPEKKSYF
                     DTIIEKLFELDQVEDLIIVLSQTIQVLIIDHLHVVGDIYDRGRYPDRILNRLMAFPNL
                     DIQWGNHDVTWMGAASGSYLCMVNVIRIAARYNNITLIEDRYGINLRRLVDYSRRYYE
                     PLPSFAPILDGEEMTHPDELDLLNMIQQATAILQFKLEAQLIDRRPEFQMHNRQLINQ
                     VNYKDLSISIKGVVHQLKDFNSRCIDSKNPSRLTSEEEELLQQLMIAFQTSESLKKHI
                     DFLFEKGSMYLTYNDNLLFHGCIPMHSNGDFKSFKIAGKTYGGRDLLDLFESQIRLAY
                     ARPEKHDDLATDIVWYLWCGENSSLFGKNAMTTFERYYVSDKVTHQERKNPYFKLRDK
                     DDICTALLQEFDLPKFGHIVNGHTPVKEKNGEQPIKANGKMLVIDGGFAKGYQKNTGL
                     AGYTLIYNSYGIQLISHLPFTSIEEVLSGTNYIIDTKRLVEEAKDRILVKDTTIGQKL
                     TKEIKDLDHLYRHFQEYDD"
     gene            53703..54827
                     /gene="queG"
                     /locus_tag="VBE34_000182"
     CDS             53703..54827
                     /gene="queG"
                     /locus_tag="VBE34_000182"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEO7262939.1"
                     /translation="MDIKAEIQKLAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRIYPERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPNSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNPLATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILEFMSNLTLKDEDSRKELELIRHKWQF"
     gene            54896..55994
                     /gene="prfB"
                     /locus_tag="VBE34_000183"
     CDS             join(54896..54967,54969..55994)
                     /gene="prfB"
                     /locus_tag="VBE34_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEO7262940.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENQMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            56013..56705
                     /gene="ftsE"
                     /locus_tag="VBE34_000184"
     CDS             56013..56705
                     /gene="ftsE"
                     /locus_tag="VBE34_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEO7262941.1"
                     /translation="MALIEMSGVTKKYRRSTTALRNLNLSIQQGEFVYLVGPSGAGKS
                     SLIRLLYREEKLSSGRLKVGEFNLNKLKRRQIPILRRSIGVVFQDYKLLPTKTVYENV
                     AFAMQVIGAKRRHIKKRVPEVLELVGLKHKMRSFPTQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEIAWEIMHLLERINLQGTTVLMATHNSQIVNTLRHRVIEIEAGSVI
                     RDEEKGEYGYHD"
     gene            56689..57618
                     /locus_tag="VBE34_000185"
     CDS             56689..57618
                     /locus_tag="VBE34_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7262942.1"
                     /translation="MVIMINFFRHFWESLKNLKRNFWMTFASVTSVTITLLLVGLFSS
                     VLLNVEKLTTDVSGNFTISAFLNVDSTDAQKQVKDKDGKLKDNPDYHKVYDKIKRISG
                     VEKVTYSSKAEQLKEVQKEYGSDVIDDTYKDALLDVYVVGTSSAKVSKSVSEAIGRIE
                     GVDYTKEPIDSTKLSNLTDNIRIWGFGGVALLIVLAIFLISNTIRMSIMSRRTDIEIM
                     RLVGAKNSYIREPFFFEGAWVGILGAIVPSLIFYFGYQFVFNKFNPKFETSHVSLYPM
                     DIMVPAIIGGMVIIGIIIGSLGSVLSMRRYLKI"
BASE COUNT        19557 a         9150 c        11535 g        17393 t
ORIGIN      
        1 aaaatcggtt attaatatta atttgcaaga tttaaaaaat aaagaaggta tttatttgaa
       61 agcgactgca attgatttag taaacgataa ttttagtttc gatattttta agaaaaagcc
      121 ctaaaatgac gttaaatact tgaccttaag ccttttttta gatatcatat aatctgttaa
      181 aacttatgtt gtataagttt agaatttatt tggaggattt gttaacatgg ctaacaaaca
      241 agatttaatt gcaaaagtag cagaagctac agaattaact aaaaaagatt cagcagcagc
      301 agtagatgca gtttttgcag ctgtagcaga ttaccttgct gaaggtgaaa aagtacaatt
      361 aatcggtttt ggtaactttg aagttcgtga acgtgcagct cgtaaaggtc gtaacccaca
      421 aactggtgca gaaatcgaaa ttgcagcatc aaaagttcca gcatttaaag ctggtaaagc
      481 tcttaaagac gctgttaaat aattcgtcta gaaaaacctt gtcatatcaa tgtttattga
      541 tagcgacaag gttctttttt tatatttggg gcatatttgg ggcataaagt tagtagtttt
      601 ccataatatc agctatgttg gatttcattt tctttgtaat gtgggtataa atttgtacag
      661 tagtttttgc atcagcatgt ccaacacgat ccatgattgc ttttagtggt acgttatttt
      721 cagcaagacg actgactaaa gtatgacgga agatgtggct agtgatattt ttattgattg
      781 gttgttcaag ccgctcgttt gcttttttca atgctaaatt aaaggaattt atttggatag
      841 gaacaccgtt cctagtcgtg aatatatacc ccatgtctct gtatcgttta tttgtatttt
      901 tttcgagttc gttaataaac tccatctctt gtaaaatttc catttcacgt ttgctcataa
      961 tggtttcacg ataggacgca agggtttttg gagaggtctt ttctccattt atgtaaccat
     1021 tagtgcgatc ataagttccg tgcaactgta gagttttgtt tttaaaatca atattatccg
     1081 gctctatgct aatagcctca ccaatacggc atccgttaag actcataaat tctgatagaa
     1141 atcccaggcg gtgagtgcta ggtcttctaa ataattcttt taacaaccgc ttgatttctt
     1201 cttcttcaag atatttttct tcaatctttt tccaatcatt aagtgttttc ttgatttttg
     1261 gcagtttagc gcgtcttgca ggattatcct tgatgatatc tagactaaca gcataatcaa
     1321 agataagatt aagcatagac ttgtttcgct cttttttgtt tctagagcaa tcgagattat
     1381 ccagataatt ttggacatat ctagggtcga tattaactac aggtatattg atcccaaatt
     1441 ctgctctaat ctctcgaata ttgcctttta aagtgacaat agacgatctt ttaatttcct
     1501 gttggtaaaa tatccaccac tcatcaatta gatttgtaaa tagcatgtca gaggtttgca
     1561 actttcttac gatatcagct atcttagcat ctaatattct ttgagcttct tttctaattc
     1621 gaggagtatc tttttccatc agtactgatg tacgtttcca tttattagta tatggatctt
     1681 tatatctttc tacaaaattt acttttccac ttttgtgttt ttctgaccac attgattttt
     1741 acctcatttc ttgataaaat gggtacaata aaaagggctt tttaatgcct aatttattgt
     1801 acagtttgcc tcacttcatt gcttgccggc tagagtgggg ctttttctat ttgctaaaaa
     1861 aattttgaat attgttagct tgagcctgag ttagttttgt tttaattatt tttatatcat
     1921 ttgtttttaa atttctgagt gttaattgag ctttacccgg aatttcctcg gttatagtag
     1981 tagaggtact gttaatagtt ccttttttac cacgatttgc accagctaat ccgccaagta
     2041 ctgcagcagt aggattgata gttgcaccta ttaatcctcc aataattgta ctaccagaac
     2101 gacctttttg agtcgttttt ccttttgtta ctgttttttc aataattcgt gaaccatcaa
     2161 actgaaaatc cacaaattta aataaatcag gtttttcaga ataaaaacca atatagtagt
     2221 caccctccac tgttttacga atagcagtag gtgtattaaa acctaaatta attgtgggcg
     2281 gtaagactat tttgctttct tgtgatgccc tttttctcat ttcgtttgct ttaataatgc
     2341 cgtctttagc cttttctgat gttgacacag caaattcctt tattttattt atatccatat
     2401 aatctcctca ctatattaca cgaccttaag taaattatag tattcatcaa ttaccatgca
     2461 ttcatctgca gtagttgtta gttgatgata ttgcataaat tgtaggtagt taaactctcg
     2521 tttatcttct aattgagaaa gtgcttcagc gattaaatga ctaatcatgt atctgtcagc
     2581 ttctagctcg tgtttctcac gtagtgtctt atagtcacca atatttcttt cacaatgtcc
     2641 gagttcatgc aggataaccc gtttttgagc atctaacgat agtgatttat tgacaaaaat
     2701 aactttaata tcgtctatat aaatcccagg tcgtggccat aattcgttat caaagtatgc
     2761 gagattaact ttgtgacagt ctacaatctc ttcaatagtc atagactatc ttcctttcaa
     2821 ataaatttca ataatatttt gtattgcatc aatatctgat tcagtaagtg gcttgccatc
     2881 aaatgtttta gccttttctg ccatttttct aagatcgtca gaagtgtatt cttggctatc
     2941 tttagctata ttgggattat ctgttctacc taataagtaa tctgtagaga cattgaagta
     3001 gtctgctatt tcagcaattc tttcagcgtt gggtttttga gttttcaact tatagagtgt
     3061 atttcggcta tatcctaagg tttcctctag ttgaataagt gtaattccac gctgtttgca
     3121 caattctttg attttttcaa acgtcggaaa cattgattta tcaacctttc taaggcatga
     3181 caaaaaatat ttcaactttt tgggtgaaaa atgttgacaa ttcaaccaaa taggtgtatc
     3241 atattttttg taagcaagag ataactaaaa aaacaactaa gcaaacaact ataaaacagt
     3301 tttggcgaac gggttttagt tgatttgtta aggttcttta atatgcttta attctaactt
     3361 tttaggtgtg agttgtcaag tggttatata ctaaaaagtt gaaaatttag ttgtttcttg
     3421 tttacagatt ttaaaacaag gagggaaaat gaaaaaaaac atcgttgatc tacaaaagcg
     3481 taatgagcat tttcagtggg tcgctgatag cttagagggg aaagaaaacg aactttacgt
     3541 ggaaagagat tggtacgaca atccgactct tatttccaaa gaggatgcaa aaaaagaagt
     3601 agagcaagta caacaaaaac ttattctact tcaaaagaaa tcatttattg aatatatttt
     3661 gcaattattg caccaactat tccgccgaca ataaggttga ttataccgat tatcactttt
     3721 cctgaggttg tttcttgaaa agcggaaagt aatccagata ggttagttcc tctcattttc
     3781 catttagggg tgttagtttt taaatcatcg ttgatagcta aagtgaggaa tgttgaaaag
     3841 ctattgacct tgcttgatgt tgacatactt agttcatggt attgtctcga agagaaagcg
     3901 tcagcttctt gtttacctgt cagaaaacga tgtggggtta gattgttcaa ttcaagtaat
     3961 ttttcaatat gcggattgtc aatatatcct ccaccgtaca tctttttaaa cagataggct
     4021 aaatcgatct ttttgtcatc atcaatgatt attggtgtgc cgcctagaac tttgaatcgc
     4081 tgctcaatag catgaaagcc aaagacagta tctctcatgt tccagtgaat ccaatttttg
     4141 tcggtatttc tgtcaacaaa tgtgaagaaa tctgttaaca gctctttttc gatagtttca
     4201 taagtatcat cagtaattgg ggtatctaat atctcggaat actgatgaat ggaaaagttg
     4261 ttggtttggg cattattgaa ttgacgaact gaaatggaag ctattctagg acttttacta
     4321 cctagattat aaaaactttc gcaggagtaa tggataatta gatatttttc tggtcgttct
     4381 tcaatacttt taaggatagt attagcgtat ttataatcag agtatcgaga catttttacc
     4441 tcaagttttt atttttatta tatcagaaaa gaaaggagaa aatatgccag atattgcagt
     4501 tggacgagaa aaagttgtta cctttctaaa aagtaacaat atcaagaaga gtgatttagc
     4561 agcagcctat ggtctcaatc gtcaagaggt cactaacatt cttagtggct caactagagg
     4621 accaaaggct aaccagttta ttctgcgagt gattgcagat tacagcattg agtaaatgca
     4681 aaaaaaggaa ctgattgcag tcagcccctt atcaaaaatt ctacttacat tatatcacag
     4741 attggaggta agaaatgcca aaagctgaat tagtctacag accagctaag cagtcggaaa
     4801 aagccgacta tggcgattat gtgcatcttt gccaaatctg ggagggatta accgtcggaa
     4861 cggcaaaggt ttgggcagca gaaatgagaa atcatccaga ttttaaacaa tttatcaata
     4921 atccaacgca tagaattgta ttcatcaatt acgagggatt taagttgttt gttacttgga
     4981 agtctagaaa tagatataaa cctaaaaagg aaacgctggc agaaatgttg gaaaatatta
     5041 aaaaggaaag acagttagga gtataacata atgaagaaat tatttaaaaa attatttgta
     5101 aagaaaaatc aggttataga aacacgtcaa aaatggacaa tcgaaacaca tggctgggaa
     5161 gctaatgcac gacgttacga caatatgatt gaacgtaata atagagggcg tacatgttaa
     5221 aagaagtatt actagaaaat gaactgttgc gtgatgaaaa tagacgacta actaatgaaa
     5281 tggcagatta ttattttacg aacgtagcaa aagcaaattt attggatatt atcgtcaacg
     5341 aaggttacat cttagatagc acactggaaa aatgtattgc taagttagat aacgttgata
     5401 gacaaacact agaaaaaaat tggagtgatg ataatgattg aagttgaaag acaaccaaaa
     5461 aatacgtact cacgaattag atgttcaaat aatatttatg atgaaatcgc caatattgca
     5521 aatgaatgtg atttaacact aaaagaagta actgatgcac tactaaacta tggtcttgct
     5581 aacgctcaaa ttattagttc tgaaaaagta gttaccgaaa gtaaattagt aataggagat
     5641 tttgacaatg acaatcacaa ttaataagtt agaaatcgaa aacgtaaagc gcattaaagc
     5701 tgtaaaagtt gaaccatcat ccaaaggatt aaccatcatc ggtggcaata acaatcaggg
     5761 taaaactagc gttctggata gtattgcttg ggtattaggt ggtaataagt ttaaacctag
     5821 tcaagcagca cgagagggaa ctatggtacc acctacgctt aaaattacca tgtcaaatgg
     5881 tttgattgtt gagcgtaagg gtaagaattc tagtttgaaa gttattgatc ctaacgggca
     5941 aaaaggtggt caacaattgc ttgatagctt tgtagaagag ttagctatta acttaccaaa
     6001 atttatggat ggaactccaa aagaaaaagc agatgtctta cttgaaatca ttggagttgg
     6061 tgatcagttg gcagaattag aactcaaaga aaaagaactc tataatcaac gtcatactat
     6121 tggtgttatt gctgaccaaa aagaaaagtt tgctaaggag caaccatact ataatgatgc
     6181 cccaaaagaa ttaattagta tcgctgattt aatccagcaa caacaagaag tgttagcaaa
     6241 aaatggggaa aacgctagaa aacgtcagaa tctatccgtt attcaacaaa atcatcaatt
     6301 taaacagtca gaggttgaac atttaaaaca acaattagcc acagcagaga aacaattaca
     6361 agagttatct agtgacttgg aaattgcaca aagagacaca atgtcattaa tagatgaatc
     6421 aactgcagaa atagaagaaa acatttctaa tattgaagaa gtcaaccgca aagtcagagc
     6481 taatctggat aaagataaag ctgaagaaga tgctaaacat cagcgtgaac aatataacat
     6541 attgactaat gatattgagt caattcgtca acaaaaacga gacttattga ttaatgcaga
     6601 tctgccactt gaaggcttgt cagtagatga tggaaagtta ctttaccttg gtcaagagtg
     6661 ggataacatg tcaggcagcc aacaactaat agtagcaact gctattgtac gtaaactaaa
     6721 accagattgt ggctttgttt tgattgataa actcgaacag atggacaaca tcacacttga
     6781 acaatttggt aaatggctag agcaggaggg tttgcaagct attgcaaccc gtgtatcaac
     6841 tggcgaagag tgtgccatca ttatagaaga tggatactca gtggaaaata aagcccatag
     6901 ctttaaaaca gcagagggtg gtagttttgc tgaaacagta gcaccgactt ggcaaggtgg
     6961 attttagaaa gagaggtaaa tatgcaaatt actagaggaa aacgggcaag ggcacaacgt
     7021 gtcattatct atggaccaga aggaattggg aagtcaagtt ttgcagcgca atttccagag
     7081 ccgttgttta tagacacaga gggttcaact gacaacatgg acgtggcacg catggacaag
     7141 cctacaagct acactatgct aaaaaatcaa attgcttttg taaaagctaa ccctacatgc
     7201 tgtaagacac ttgtcattga cactattgac tgggcagaaa gcttaatagt agatgatgtc
     7261 tgtgctcaac atggaaaaaa aggaattgaa gattttggtt ggggtaatgg ttacacttac
     7321 acaaaagaag aagttggacg tttcctaaat atgttgcagg aattgataga gttaggtatc
     7381 aatgtcgtcc ttactgctca tgcccaaatg cgtaaatttg agcaacctga tgaaatggga
     7441 gcttatgatc gctgggaact caagctaggt aaaaagacaa gctcacagac tgcaccatta
     7501 gttaaagaat gggctgacat ggttctgttc gctaattata aaactgtagt gatgacagct
     7561 gataacaaca agaagaaagc aactggcggt acccgtgtct tatatacaca acatcatcca
     7621 gcgtgggatg caaaaaaccg tcatggatta caagaagaaa tgccacttga ttacgctggt
     7681 atcgcacata tattcgcaca agcaccaagt caaccagtag cagaaacacc gccagtacag
     7741 caaccggtgc ctactcaacc tatacacgaa cctgtacatg agcaacaggc gcaaatgtcg
     7801 ggaccaactg cagcaacaga gtatccatct gtcctaccaa aggctttgac ggatttgatg
     7861 acgcctgaac aagttacacc agatgagtta gtagcagtag caaatattag aggacacttc
     7921 cctccattga ctcctattga aaactttcca ccagattact ggaacatgat tgtagcaaat
     7981 tggggagcca cattggaagt tattaaaaca caagtccgag caacagaaca gccctttaca
     8041 gtggaaggct catagatttt gggaattaga aataatagca aaatataaca aaaattggat
     8101 ttattccaat agctaacaat actttttaaa attaaaatag gagaaataaa atgactgaat
     8161 acaataacaa ctttgaacgt gaatttggat gggatgacac aatccaagaa gatgcaaaag
     8221 aatttattac attacaacct ggtgactatg tcttcacagt aacagacttt gaacgtgcac
     8281 gccacacacc aaatccacaa aaaacaggga aattgccagc atgtaataaa gcagtaattt
     8341 ctcttgaaat tgaaactgat gaaggtgttg ctagtttgaa gcataatcta tttttacata
     8401 gctctaccga aggaatgtta tcagcattct ttggggcaat tggtcagaaa aaacacggtg
     8461 agccattaaa aatgaattgg aacacagtag ttggtgcgac gggtgtatgt agtgttaaga
     8521 atagaacata taacgacaat gtttataatg aagtgaaatc gatgatttat gctgatagcg
     8581 ttgactggac taaagtattg aatgcaaata ctcaaccaca agcgccacaa ccaacgtatc
     8641 aacaaccagc tcagcaaaca actaattttg cacctcaaca acaaccagca cctcagtcat
     8701 atcaacaagg tcaaatgcaa gcatctcaac aacaaggtgg atggggagga ttctaatggg
     8761 aaaagctaaa gattacactg gtcaacgatt tggtaggctt gtagtgattg agcgtttacc
     8821 tcaggatcca tatacaaaag caaaatataa atgtcaatgt gattgcggta agttaacgat
     8881 tgttaatagt agtaatctag caactggtca tagtaccagt tgcggatgca ttgttactaa
     8941 acatggtttt gctagaaaag agcgtcttta taatatttgg gttggaatga gacaacgctg
     9001 cagagataaa aattccaatg attatattaa atacggtggc cgtggaataa ctatatgtga
     9061 cgaatgggaa gaatatattc aattcaaaca atgggcatca tcgaatggtt atcgagacaa
     9121 tttatctatt gaccgtattg atgttaacgg aaattatgaa ccatctaatt gtagatgggc
     9181 taattcaacc gaacaagcaa ataatcaaag aaataaccat ttaatcacat ttaaaggtaa
     9241 gactcagaca ataaaacaat ggtcaagtga acttggtatt tctaatatta ctttacttac
     9301 aagattaaat cgtggttgga gtattacaaa agctttaacg acacctacac aaaaaggagt
     9361 gtgatttaca tcaaacttag agaatatcaa gaagaggcaa ggtcagcagt gcaacaagag
     9421 tgggaacaag gcaagaagaa aacccttctt gtccttccta ctggttgtgg taagactatt
     9481 gtcttctcaa agattattga agatagagtt aaactaggcg aacgtgtttt agtattggct
     9541 catcggtcag agttgctgga acaagcaagt gataagctaa tgacagcaac aggattgggc
     9601 acggctttag aaaaagcaga aagtacatcg ctcggctcat ggtatcgagt ggttgttggt
     9661 tctgtgcaga cgttgcagcg agaaaagcgt ttgagtaaat ttccaacaga ttacttcgac
     9721 acaattgttg ttgatgaagc gcatcatgct atttcagacg gttaccaaag agttctacaa
     9781 cactttgata gtagcaatgt tttgggagtt acagcaacac cagatcgtgg tgacaaacaa
     9841 aatctaggta aatatttcga tagtttagct tatgagtatt ctttggtaga tgctatcaaa
     9901 tctggttatt tatcaaaaat tacagcagtt actattcctt tgactttgga cctatctaca
     9961 gtgagtcagc aagctggaga ttttaaagca agtgagattg ggacggcgct tgacccgtac
    10021 ctagaacaaa ttgccgatga aatggttaag caatgttcta atcgcaaaac agttgtattt
    10081 ttaccacttg ttaaaacttc ccaaaaattc cgtgatattc taaaccaaaa aggattcaaa
    10141 gcagcagagg tcaatggaga atcgaaagac cgtgctgaag tcctagaaga ctttgataat
    10201 gataaatata atgtactatg caattcaatg cttttaactg aaggatggga ttgcccgaca
    10261 gtcgattgcg tagttgtact gagacccaca aaagtaagag ctctttatag tcaaatggtc
    10321 ggtcgtggca cacgattagc gccggggaaa gaaaaactac tgttattaga ttttttatgg
    10381 cataccgaac gccatgagct ttgtcgtcct gctcatctaa ttgcaaaaac accagaagtt
    10441 gcacaaaaaa tggtcgagaa catgaccgat gaaactgaag tagcttttga attgttagaa
    10501 gctgaagaag tagccagcaa agatgttgtt gcagagcgag aagaagcact cgctaagcag
    10561 ttagcagaaa tgcgtaaacg taaacgtgca ttagttgatc cgttgcaatt tgaaatgtct
    10621 atcaacgctg aagacttagt ggactatgta ccggaatttg gttgggagat ggcgccagct
    10681 tctgaaaaac aacttaaaac acttgagaaa tttggcatat ttactgacga aattgggaat
    10741 gctggtaagg ctgccaaatt attagataga ctggataaac gtcgtcaatc aggtctaaca
    10801 actccaaaac aaatacgaat gcttgagcga tacggtttca aaaatgtagg tatgtggaaa
    10861 tttgaagtag catcaaacat gattaatcgt attgctgcta gcggatggcg cctaccaaaa
    10921 ggcatcaatg ctcgagaata tcaaccagaa taggagaaaa aatggaaaat attaatgaac
    10981 agattgaaaa gtttatttca aattttgctg atgaagcaat cgaaaaatcc gaaacatatt
    11041 cggaggcaat tttatatgtc gataaacact caagtctcac cgagtttggg caagtagtaa
    11101 aaaaagctat tcaagaaaaa atcagagata ttgccttgaa tagcagaatt attaaatagc
    11161 ttcaattttt ataacagcat ttgaagcgta ataaatttgt gggttttcta agtcaaagaa
    11221 aaattgcgag ttagcaatta attctaagaa gctaggaatt aatccatcat gaacatggtt
    11281 ccataatgaa tatatttttg ataaggacat agcatcattt gattttacta cactttgaaa
    11341 actctgttct tcgtgaaata taacaataga accatctgaa aaagtaattt ttatttcagg
    11401 catatagtgg acctcctttc tttaggttaa ttatatcaaa tgcagaaagg ataacatgtg
    11461 cagaaaggat aacatggcag agaatgattt taatttgcta ccgttgctgg attatatcaa
    11521 tcctgccacg gtagactatc aaacatgggt tcaagtagga atggccctaa agcatgaagg
    11581 ttatacagca atggattggg atgtttggtc acaatctgat agtagatata aaaaaggtga
    11641 gtgttttgca aaatgggata gtttccaagg caatggcttt gggactatca caggggcaac
    11701 aatcacacag ctagctaaag ataatggatg gacatcatca gagtatcgta acagcgatga
    11761 tgctcatgaa ctcagctggg acgatacaat cgatcgtgat tataagattg tcgataaaaa
    11821 ttggattgaa tcgaaagaga ttcaagagcc aagaaactgg aatccagttc aagatttaat
    11881 tacttacata gataccttat ttgaatcaac tgacaaagtt ggttatgtaa cagagaccta
    11941 tccaattacc cttgatacgg gagagattgt ttataaacca acaaaaggag cgtatgacag
    12001 gactgctggt caactgattg aatcactcca aaaaaatcct actgacttag gagcagtatt
    12061 tggagacttc aaagaagagg ctggtgcatg gattcgtttt aatccactag atggaaatgg
    12121 tgtcaagaat gacaatgtaa cagactttag atatgccttg gttgaatccg acagcatgga
    12181 acttggtaaa cagtatgctt tgtttaaaga actagaattg ccaatagcaa ccttagtcca
    12241 tagcggtaaa aaatcattac acgctattgt caaagtagat gctcgtgatt atcaggagta
    12301 ccgcaaacgg gttgattata tctatcaaat ttgtaaaaag aatggacttg atattgacac
    12361 acagaaccgc aaccctagtc gattatcacg catgcctggt gtgactcgga atgggcacaa
    12421 gcaattcttg attgatacta atgtgggtaa aaccaactac gaagaatggt atcaatggat
    12481 tgaagattta aatgacgatt tgcctgaccc agagacgcta gctgacgaat gggataatat
    12541 gccagaattg gcaccggaac tcatcaaagg agttttgcgt caaggccaca agatgttgat
    12601 tgctggacca tcaaaagctg gtaagtcgtt tgcattaatt gagctatcca tcgcgctggc
    12661 tgaaggtaaa gaatggttag gctggcagtg tgaacaaggt aaggtcttgt atgtcaatct
    12721 ggaactggat agaccgtcag ctttgcatcg cttccgtgat gtatacgaag ctatgagctt
    12781 gccaccagca aacatcaaga atattgatat ttggaaccta cgtggaaaga ccgttcccat
    12841 ggacaagtta gcgcctaagc ttatccgtcg tagcttaaag aaaaattacc aagcggtcat
    12901 cattgaccct atctataagg tgctgactgg tgatgaaaat agtgcggacc aaatggctca
    12961 ctttaccaat cagtttgaca aagtagctac tgagttaggt tgtagtgtga tttactgcca
    13021 ccatcactca aaggggagcc aaggcggtaa aaaatctatg gaccgtgcaa gtggttcagg
    13081 agtgtttgcc cgtgaccctg atgcactgat tgacctagta gagctagaat tgactgagga
    13141 actcatcaag tcacgctcag aaaaagcagc cgctaagatt taccaacaag ccttgcaaga
    13201 aaaagcgcta gcctactatc aacaggaagt aacgctagat gatttggaaa gtcgttatca
    13261 gatgcagcaa cattttgaca aggctatcaa ggacatcatg attaaacagc cctatctgga
    13321 agcggtcaag aaagcccagt atgaggtgga gatttccact gcctggcgag ttgaagggac
    13381 tttacgcgag tttgctaaat tccaaccagt taacatgtgg tttagctatc cagtgcatga
    13441 tgtagacaca acgggtgtct tggctgatat atcactagaa gataatgtgc cgacttggaa
    13501 gaagaatttt gagaagaaga gtccaagaga gtccagggaa aagaaatctc aaaaagtaga
    13561 gactgcaatt aattcattga atgatggaat agagccagtc acaatcgata acttaatcga
    13621 atatttttct actgaagata agccagtttc tgaaaaaact attcgtagat ggataaaaga
    13681 aaacggtaaa tttgaagtta aaaataaaca aattttacca atagaagaac ctaaaaccaa
    13741 taaaagtttg tgaatatttc acagagggac agggacaggg acagacaaaa tcgagggtca
    13801 aattcgaaag tgtccctcga aaatgtccct gaaaaatagg gacaaactcg aaaatgtccg
    13861 tgtccctatt acatgatttt cagggacaaa aatagggaca aactcgaaaa tttctcgaaa
    13921 atgtccttga cttttgaggg acaaaattga gggacagaat attctctttc tccgaaagaa
    13981 gaatatttag gaaatgtccc aattggtcaa tgggtacatg aacaggaaca agggggctat
    14041 gcatccgccc cttgtaaccc tgtaaccatg tcccttgaca ttgactatgc gcgaaaataa
    14101 aaaaagatgg gaagtgacaa aaatgaaaga atacaaaaaa agaatgattg ttaaatatca
    14161 agaattaaaa agccgtgcag aaaaattatc agtattaatt aataaatttt atattgatag
    14221 tctagatttt aaattacaat gtcctattga attattagta gcacaatggc atgcaatggg
    14281 agcttatcta aaaattttag aacaacgtgc agcaatttga aaaaataaac ttggataatg
    14341 ttgatcatga caattgaatt ttttatccca atgaaaaaaa taccaaccac aacacatcag
    14401 caaaaaaagg tccgtgtggt aaatgggaaa ccacaattct atgaaccaga ttcactaaag
    14461 gaagctaggg caatgtttat ggataagttt gctcaacatg tccctaaaga aaaattaaat
    14521 ggtccaataa gactaacaac caagtggtta tttccgaaaa tcaaaaaatc agtaaacgga
    14581 caatataaag atactaaacc cgacacagat aatttgaaca agttacctaa agattgcatg
    14641 actgaattgg gattttggaa tgatgatgcg caggttgctt cggaaattat tgagaagttc
    14701 tgggctgata cagtaggaat atatgtgaag gtggaagagt tatgaaaatt gattatattg
    14761 atttttttga acgagtggtt cctggctgga tgagagaaag taacaaaaaa atgcaggagc
    14821 ttggttttgg aactgaacaa tattgggtat gggttaatat gtcaatcgtt gatatttgcg
    14881 agagttatgg caacgacgat ttggtgaatg gccaattcca catgatttgg gagtggctag
    14941 aaaggaaaac aaaatgacag gtgaattagt acataaacca aagcattata atcatgggga
    15001 aattgatttg attgagtcgt ggtataagac atacccatgg gaccagtttg tcgcaatcat
    15061 ggaaagtcac attgataaat acattaaacg acatagatac aaaggcaatg cgactcagga
    15121 tttagaaaaa gcactcgaat atacaaaacg tctcgttgaa tattggttac tagaaagtgt
    15181 gaacgaatga acgaacaaaa caacaaatta cacaaacagt caattgtgat tgttgtatta
    15241 actgtagcag tcagcgtgtt gttaacaaca aatattgcat tgtacaacta ctaccaacca
    15301 caaatcacag gtttacaaca gcaactaata cgcacgcagt accgtttgaa agagtcgtca
    15361 gagcaaaatc agagacagac aaagcggatt gcggagttga caggaaatgg gggataaagt
    15421 tgtcattaat tgatgaagta aaagaatgcg gttcagaatc tcacaaaaaa tggtttgata
    15481 ggtactttga tggtttaaaa atcgaggaga aacttaaaca gtctgcgatg aaaggattct
    15541 ctggttataa aatctctatt ccaaaaaatg atgaatactt agctagacga cttgacagcc
    15601 aaaaaacaat agatttacta aaataccgac ttggtgatgg ttttgatgtt gaaataaaaa
    15661 atcttgagtc atcatataaa atctttggta aaccagttgt actaagtaga gatttattta
    15721 ttttttggag aacaggagat aagaatggat gataacacag cagttgtttt gatactttta
    15781 atattatttt taccgtttat tatcggcagt ttagagaaga ggtgacggaa tgagtaaatt
    15841 atctaacgca cagttaaagg cttttgatga gtggttgttt gattatcgcg atattgaacg
    15901 taaaattgca attagaaaac ttgagctgca gacgcctgtt agtacagata tcaatgtagg
    15961 cggggggaag tctaatctag tagctaaaga gacagaagat attgtcataa agtggtcaaa
    16021 ggacggtaaa atcaaaagtt atgaaaactt cagagaaagt gttgataggg ttaaagcact
    16081 gcttgacgat gaattgaatt atatttttga tctaaggtgg ggaactggtt ctaataatac
    16141 ctgggaggag atagcttata agattcatac gtctagagca ggaacctacc gaaaaaggga
    16201 tagaattttg actctatttg cacaacaaat tggaaaattg taagtttgag acaaaaaagg
    16261 gtggttttct cacttttttg atgataaatt ggtagtatga gtttgaaggc aaggcaaata
    16321 gtgtcttgtc tttttattat gtcacgaagg aggtgatgga aaattgagta aattaacact
    16381 aaaacaaaag cgttttgcag atgagtacat catctcagct aacgcgacag cagcggctat
    16441 taaagcaggt tacagtaaaa agacagcgag gtcaataggt caagaaaact tgaccaaacc
    16501 tgacattaaa gcttatatag atgaaaggat tgaaaagctt gaatctgcaa agattgccac
    16561 gcaagaagaa gttttacaat accttacatc aatcatgcga ggcgatcagc aagaaaaaac
    16621 actgatcagc atcggggagt taggacaaga gattgtcgat attgatgtag gtgcaaaaga
    16681 tagaatcaag gcagctgagc tactaggtaa gcggtacaga ttattcactg acaaaattga
    16741 aatggatata agttcagatg taaccatcag tgtaggtgag tgggatgacg actaagcaac
    16801 gccctaaaat taatattgtc atcaagtatc ctagcaaagt ctttaataaa catatctacg
    16861 ataaactata taattacgat aacttcacag aggtacacta tggaggtgca tcttctggaa
    16921 aatcacacgg tgtattccaa aaaattatct taaaagcact caatccaaag tttaaacatc
    16981 cgagaaagat tcttgtcctt cgaaaagttg gtgcaactgt cagagactct gtatttgctg
    17041 atattatgtc taacttgtcg tattttggcg tcttggataa atgcaagata aatatgtcag
    17101 cttttagaat aacattgccg aatggctcgg aatttatttt caaaggcatg gataatccag
    17161 aaaagattaa gtcaattaaa gggatatctg atgttgtaat ggaagaagct agtgagttta
    17221 cacttgatga ttacacacag ttgactttgc gtttaaggga taagaaacat ctagagaagc
    17281 aagtctatct catgttcaat cctgtgagta aagcaaactg ggtttataaa gctttcttta
    17341 tcaagtcacc taaaaacact gtagtttatc aaactacata taaagataat aggtttttag
    17401 atgatgttac tagagaaaat atagaggaac tagcaaacag aaacgaagct tattacaaga
    17461 tttatgctct aggtcagttc gcgactttag aaaagctcat atttccaaag tatgaaaaaa
    17521 gaatactaaa caaagacaag ttatcacact tgccttcttt ttttggtttg gactacggat
    17581 tcattaatga tccttcggca tttttgcatg tcaaaatcga tgatgcaaac aagaagctat
    17641 acatcttgga ggaatatgtc agaaagaatt tgaccaatga taaaatagct aatgctataa
    17701 agagcctcgg atatgccaaa gaagaaatca gaggggattc agctgaaaag aaatctaacc
    17761 aagagttgag aaatttaggt attcaaagaa tgattgatgt aaaaaaagga gctggttcag
    17821 ttatgcaagg aattcagtac atgctacagt acgatatcgt tgttgacgaa aggtgtgtta
    17881 aaacaattga agagctggag aattacactt ggaaaaaaga taagaaaact aacgagtata
    17941 tcaatgagcc agttgacagc tataaccatt gcattgatgc tatcagatat gcagtacaag
    18001 atagaatata tcaatcatct gacagaaata aacgactaaa taatgctaaa tattatttta
    18061 gatagagagg gatttgaact tgtttgaaag aaaattttta tcaggaacaa gatttaatga
    18121 aaatgcgaac aggcaattta tcatgttgca agaagatttt gatgctattg aatataattc
    18181 gcagaaatgg attgatcagt taaaaaaata tatttctagt tttgagactg agcaactaga
    18241 ccgattgaaa gagttaaagc gatattatct tggtgataac aatatcaaat atcgtccagc
    18301 aaagaccgat gaatatgcag ctgacaatcg tattgctagt gattttgcta agtacattac
    18361 agtctttgag caaggttata tgcttggtgt gcctgtcgag tacaagaacg aagatagtga
    18421 tattcaaaaa aacattaatc ttatgagtgt tcgtaacaac gaagactatc acaatgtagc
    18481 aatcaaaacg gacttatcca tttacggtcg tgcttacgag cttttaaccg ttgaagaagt
    18541 agataatggg acagaaatta aactctatca actacccgca gaacagacat ttgttattta
    18601 tgatgatact tatcaacata attctttgat ggctgtacat ttctacgaca tcgattacgg
    18661 cgcaggtaaa cgtaagcaaa ttgttaaggt ctatacttca aatatgatat ttacttacga
    18721 ggactttaat caagaaacaa aaggaatgag tctgaaagat gaagaagaac attactttag
    18781 tggtgttcca gttaatgaat attctaataa tgatgataga gcaggcgctt atgaatcagt
    18841 cttagataat attgatgcat acgacttatc acagtcagag cttgctaact ttcaacaaga
    18901 ttctgtagat gcaatcctag ttctctctgg taatccgtat acaggagcag acgaaaatga
    18961 tttttttgaa gatggacgta taaatccaaa cggtcgttta ggtatatcta ttggattcaa
    19021 gaaggcaaaa gttattgtcc ttgatgacaa tcctaacccg gatggtgcta agccagatgc
    19081 gtattatcta aaaaaagaat atgatatggc aggaagtgaa gcgtataaaa acagactagt
    19141 gtcagatatc ttacgcttta cttttactcc cgatattcaa gatttaaaat tctctggtgt
    19201 tcaatctggc gagtcaatga agtataagct aatggcttca gataattacc gtgaaaagca
    19261 ataacggttg ttcaaaaaag gactcatgag acgtttacgt ttagctgcca atatctggtc
    19321 aatcaagggg aacgaatcga cggtatatag tcaagttaat gatactagta tagtatttac
    19381 tcctaatgtg ccgcagaatg ataatgaaat tgtttcggct gcacagagtc tttatggaat
    19441 tgttagcgat caaactattt ttgaaatttt aaacgcagta actggtgtcg atgctgaagc
    19501 tgaattaaaa cgtctgaaag aagaggctga cgagaagcaa gtaatgccag aaccaagaaa
    19561 tgtaggtgat gctaatggac aagaagaacc aacagcagga ccatcttgat tactggcaaa
    19621 aacgtcaaga agatattgtt agttatttag atagagcaga cttagatatt ttcaatgaat
    19681 tatctagagt atatagtaac caatcattgt ctttacaaaa ggatttatat gatttttata
    19741 atcgttttgc tgaagataat aagataactt atcaagatgc tgttaagaag cttagacacg
    19801 aagatttaag tgattatgca gcaaatgcca atcgatatag agagcaagct gagaaagacc
    19861 cagagctact taaacgtatt aatgaacaat atgtatcagc gcaggctact cgtcttgaca
    19921 tgcttaattt agagcttata catcgtgcag gtactatgag aggtatctta gatggtttgt
    19981 ttagcgatca tctaagaaag gcagctagtt atgcttacca aaaatctgta ggtggtcgct
    20041 caggaacaat taatgggcca gctctagaac agttaatcaa gacaccattt gacggctata
    20101 attactcaga gcaactctgg ggaaatgttg ataatctagt aaaagattta caagaagcat
    20161 tcaaaaaagg atttgtcaga ggtcttggtc ctagagaaat ggctcgtgaa ttgggcaaac
    20221 gttacgacgt agccagacat agggctgaaa cgcttattcg tactgatggc acgatgatta
    20281 tcaataatgc tacagccaag cgatacattg atgcaggtat taagtattat cgagacttag
    20341 ttcagctaga tgatagaact actcagatat gtcgaagtaa ggcaagagaa aataaaaaga
    20401 aactcttatc tgaaatacag cccggaatta atgctgctcc atatcacttt aattgtagga
    20461 ctacaattat tcctgacgag gatgaactta ataccgatgt tgaacctatc gctgataaaa
    20521 gtactaagta ttttaaggat gtcacatcag attggataga tgggcaagag tatgagtctc
    20581 aaatatctat gctgaatgag tacactaaag gaggcggaac ttacagagtt gatggtcata
    20641 acgtagtact tgaccattca aactacgaat atagagttgc taactggtta tctaagaaga
    20701 caggattaca agttgaaatg gttccgaggg ttaatttccc cgaaaatatt aacactccag
    20761 actatttagt tgacgacgtt ccatttgact tgaaagaaat cacaggttca ggtaaaaacg
    20821 tcatcgatgg taatctacga aaagcaaaaa agcaagcacc taatatcatt tttgatatca
    20881 caaaaacccc actatctttt gaagaaatac tggggcagtt agaacatatt tataagatag
    20941 atcgcagggg tctcaatata tcaattatca aaaacaaaga cgaggtgtta gctgtagtag
    21001 aaaaagaaag acgatgaccc accgcctcca cagaaaactg cttcatgggc gttagaccac
    21061 cgtctttcac taccataagt atacaccagt atagcagaat tatcaaggag gaagatatgt
    21121 ttatttggcg aatggtatta gtagcactgg gtgtattggt gctcatattg attattggca
    21181 ttgcagcaat agcagttaag tcaattattg cagaagtgaa gaaggaagga taaagcaatg
    21241 aataaacgaa ttaagaagaa acgtaaactc gaacgagcta ttgtgatgct tatcgcagag
    21301 aatgccatgc aagcagaagc tcttagaaat caacataaaa gaattgaaca acttgaacaa
    21361 attgtagaac ataatgctca agcgacaaat aacgaacttg attatataaa gaataactat
    21421 aagaaaaaat ggaagaaata attactaatc atttagatta gtcgtagcga tacggctttt
    21481 tattatgtcc aaacttgttg atgacactaa aagctacact gtttcgtcgc aggacgtaaa
    21541 actagactat cggttggtgg cgtaaccact aaaggagaaa attatggcag aagaaaatgt
    21601 agcaacagaa actactgagc aagtcgacac tcagaaagaa gctgttgaac aagctaagca
    21661 agaacatgag cgtacattta ctcgtgcgga tatttcaaaa atgatgactg ctgagcgagc
    21721 taaatgggaa gcagaacaat ctgacgctat tgaaaaggca cgtactgagg gcgaacgatt
    21781 ggccaaaatg tccaaggatg aacgtgctaa ggaagaagaa caaaaacgtc ttgatgcaat
    21841 cgcagaacga gaaaaagcag tcgcagaacg tgaaatgcgt attgagactc aaacgctcct
    21901 tgtggaaaaa ggtttgccat tagaatttat tgatgttgtt ttaggtacta ctgcagaaga
    21961 agttaagact aatattgata atttacaaat tatttttgat aaggctgttg aaaagcgcgt
    22021 taataatcgt ttgactcaga agccaccacg gactggcaac ggctctgtcg gcatgactaa
    22081 agctgagatt atggcagtag aagacgacga cgaacgtatg cgtttaattg ctgaaaatcg
    22141 taacttattt taagaataag aaaggaatat tatggctgaa aaaaatttaa acaccatggc
    22201 agacttggga gacattaaat cgattgattt tgttaacaag ttttccaaaa acattaatga
    22261 cttactgaaa ctactagggg ttactcgtcg tgaagagtta accagtgatt taaaaattca
    22321 gacctataag tgggaagtga ctttagatca aactgatcct ggagaagggg aaacaatccc
    22381 tctgtctaag gttactcgaa ctaaagataa agactataca gtgaagtggt tcaagaaacg
    22441 tcgtgcaact acagctgagg caattgctcg ccatggtgca gctcgtgcta tcactgaagc
    22501 agataagcgc attatgcgtg agcttcagaa tggaattaag gatgcgttct ttacattcct
    22561 caaaacaaaa ccaacaaaag ttaaaggggc tggtcttcag aaggcactat ctttatcatg
    22621 ggcaaaacta gctactttta atgagtttga ggggtctcaa cttgtttctt ttgtatctcc
    22681 tttagatgta gcaaattacc ttggtgatac taaagtaggt gcagatgctt ctaatgtttt
    22741 tgggatgacg ttactaaaga actttttagg gatgcagaat gttatcgtta tgccatctgt
    22801 gccagaagga aaagtttact caactgcagt tgataatctt gtttttgctt cgttgaatgt
    22861 acgtggtgga gatttaggtg gtctatttgc agactacaca gatgagactg gcttgattgc
    22921 tgcagctcgt caacgtcaat tatctaactt aacttatgag tcagtatttt ttggagcgaa
    22981 tgtactattt gctgaaattc ctgaaggagt tgtagaggca actattgaag cagataaaga
    23041 ggtcccagga gcagttccag gagggtaaaa taaatggata aggaactact tttagttgag
    23101 gtaaaaattt ttaaaggtat tgctaagaat gatgcctcac aagacgaatt gattaaactt
    23161 gccattgacg aaagtattga acgtatcctt gcgaaactca atgagtattc tgatgaagag
    23221 ctgttagaaa taccacgacg tttaacattt atcgcaagag atgtagctat taaaagatat
    23281 aatcggctta attcagaagg agccaaggca gattctgaag aaggtcggtc ttttagttgg
    23341 gaagactatc tcacagaata tgactcaatt cttagaaatg ttgcttatgg taaaaggtgc
    23401 cgtggaaaag gtgtcgctag atttatttag gaggtcataa atgatttaca aagatagagt
    23461 tatcttagtg tatgtcgatg agcaggacga ttttctagat aaaagaactg ttgaaaaacc
    23521 tagcggaaaa atcccctgca tggaaaatac tttcacaaaa tctgaacaga tggggttatt
    23581 tggtaaatat gatttgaacg cttttaagtt gcacttgcaa ggccattacg atggctttag
    23641 caagattatc tacaagggga aaccgaggtt gataaaggga ctagcgcacc ataaaaatag
    23701 cacggttatt tatgtatgag tcttacttat cgtatgcgag gactggacag gtttttacgc
    23761 agtgttgagc gcaagcagaa gtcagcacga atcgctgtag ataaaaaact tagcaaatca
    23821 gcagctagga ttgagagaca ggctaaaata ctagccccgg ttgatactac atggctgaga
    23881 agtcaaatct acaatgaaca acaaaaactc ttgcactata gagtggtttc tcctgtttta
    23941 tattctattt atcttgagtt aggtactcgt aaaatggaag ctcaaccatt tttagaccct
    24001 gccctgagaa aagaatggcc agtgttaatg gctaacctca aaaaaatgtt taagaggtga
    24061 tcaatgatat actcaccatc aactttatat ctgaatgagg taaaaaatag attgggaaca
    24121 cttaatatcc caatctattt taaattacca aaatcggacg ttttagagcc gtttattgtc
    24181 ataggttcca atgtatcgga tacatcaaaa acagcgcaga caggagctgt tgtgggagat
    24241 tttgggctaa atattgacgc attcttacct gccactagca gaacagatgc agaggaggtt
    24301 aagtctagaa tgattatgtc cttaggacga aacaaacgga ttaatgcaca gttattgatg
    24361 gataattctg taggtcgtga ggtctaccat attgctatca atatcacaga aatactaatt
    24421 taaaggagaa ttaaatggaa actacagttg aaattactac agctaagcct attaaaggta
    24481 aaaatatatt ttattttatt caatctgttg atgctgaatt aggtagtaaa gcaattctgc
    24541 cagcgtatcg cacagatggg acaacgacgc tcggtggaga ttttattgat gaacaaactc
    24601 aacaaggacg tgtgcttgaa aaagcaacag atgagcactc aattgatttg acatcatact
    24661 acacaccgaa agacaaagca attgaaattg tagaaatagc taaaacaact ggtaaatctg
    24721 tcaaagtttg gcgtgttatc gcagacgaat ctttgaaaga aaaaggttca ggaactttaa
    24781 aagatagaga agtttatcct gctaagtttg gatatggaaa aattgacgag attgaatatt
    24841 cagaaggtgt tgaagatctt gtagaagcat catatgcatt gaatattatc gatagtttaa
    24901 aagatggcaa atttccactg acggcagaag atatcgcttt gctaacaagt gtttatgcat
    24961 atcaaaaccc aggcgaaact actggcgatt atgacaatat ccagaaaaaa gaagtttcag
    25021 gaagttcacc gggagcagtt cctgaaaaat tatcagaagg acactaattt ataaggtagc
    25081 atttcgctac cttttatttt taaggagaaa tacataaatg gaattaacag tagcaaaacg
    25141 cagtattgaa attaaatttg attttaaaac aatgtttaag ataaataata gacttgggac
    25201 tgtgaataaa gagacagggg aacgtaacgc tgacggcgtc ggtacgctat tttataaaat
    25261 cttagagcgt gatgattcag ctgtagtaga tttagtcaag ctgtctgtag gcagcggtaa
    25321 aaaagcattg tcagaagatg aagtgcttga ttctatcgca gagctagtcg aagaagaagg
    25381 gtcgacagaa gccttattta aagaaattga gaatgacatg gttgaatctg gttttttcaa
    25441 agagaagatt tcgaaatata tcgaaagcat ggagaaatca gtgaaatatt tagaagctcg
    25501 agaagatgcg gacgaagctc aaatccaaat cgtaaaagac atgattggga aaatgaaaga
    25561 cgcaatctct taattaattg tgctcaactt ggcctgacgg acattgatat tatttacaag
    25621 tgtacaagaa cggaattaga tgcgattatg gaaggtttgc agtacaagca aattgcagaa
    25681 cgtgaaaacc tctctgaact tgctttaaat ctcagataca cgttaaatgc taaaaacgtc
    25741 aatgctaata agctcaaatt agataaacaa cgtaacaaaa ttaaacgaac ttatcaaaat
    25801 aaacaagatc taactaatat ttcaaacgat ttcgcagaac gtgtagcgaa gctcaattta
    25861 cattttcaaa atttataaca agatataaca agaaaggagg gatttaatgg caggtacatt
    25921 tgatggggct atattcgctg atattggtgc aaatactaaa gattatgagg cagctatggc
    25981 tagaattgtt agtactacac aaaatgcttt tagaaaagcg caggatgctg cagttaacaa
    26041 cagcaacaaa atgatacaga tgattggtca aattatggct caattagcaa ataatggtca
    26101 atcattaggg caaagactcg gtaatgcata tagtacaggt ctcaagttat ctcttggtca
    26161 aattcaaagg atagcagcat ctatcggtga aaaaattccc gaacccataa agaatgggtt
    26221 caataaagct ttttcaatta ttcaaaatag cgtacagaag ttatcgagcg ccatccctca
    26281 acccataaaa tccgcattca caagtgcaac aagtgcagta gttagctttt cgtctaagat
    26341 cacaagtgcc gtctcagctg ctttcaactc tgttagttct aaagcgagta ctgttgcaga
    26401 taaaatcagt agtagttttg ggggtagaat cacttcagcg gttactaatt tagcgactaa
    26461 gttaagcaca ggtcttagca atagttttag caggatgtcc agttctgcaa ctagtgccct
    26521 aaatggtatt agtcagagat tcgcaagcgc atcatctgct ggtgaaaaac taagaagcac
    26581 tgtgggaagt atcatttctg cttttagttt gatggccatt gctcagaaag gaatccaagc
    26641 aatcacagga gcaatggacg gtgcaataag tcgtgtagat accatgaacc gctttccgaa
    26701 aacgatggcg ctatttgggt attctgcaca acagtctaaa gcatcaatag ataaattatc
    26761 taaaggaatt gaaggacttc ctacaccact agacagtgct gttaaaagtg cccaacaatt
    26821 agctataact acaggtagct tagataaagg aacagagcta gcattagcat ttaataatgc
    26881 aatgattggt tatggagcga ctactgaagg ggctgagcaa gcacttagac agtttaacca
    26941 gtcgttgggg tctggtaaga tatatgccga agagtttaat tctgtgtctg aagctgcccc
    27001 tggcttaatg tctaaaatgg cagagtcatt cggatttggt aaaaatggtg ttcaggactt
    27061 aaaagcagct ctttcagacg gcagtattac tgcccaagaa ttcgctgata aaatgattga
    27121 actaaacaag gcgcagggtg gttttgctga gatggcccaa tcatcggctg gtggaatccg
    27181 aactgcatgg aagaacgtac ataccgccgt cgtaaaaggc gtagcaggta tgatttcagc
    27241 ctttgatgaa gctgccaaag ctaatggtat gaagaccatt gcagaatcca ttaatagtat
    27301 taagccagct ataaattctg catttgcaac tgtgaactca ataatgccaa accttgttgc
    27361 agcattcgct agaatctcca agatggtgaa tattgacttt agtccactaa ctagtggaat
    27421 taaatcagct tttggattat taaatactgc tttaggtgaa tttgcaaaaa ctggtgaagt
    27481 atcgtcagca actattgata gaatcaaatc aaagatagca tctataggtc ctactgcaat
    27541 agcagcttgg gccttattaa atcctgcaag tgctattgca actattatgc ctttaatatc
    27601 aattattggt aagattggtg ttgctcttgg gactgttggt tcagcgttgg gatcattcgg
    27661 agcaatggta gccagtggat tgtcaactgc tagcggaata attggtgctt ttgcagcaac
    27721 agtaagtggt ttacctgcca tttttgcaac tgcggcagcg agaggtgttg ctatactcgg
    27781 tgcaatgaca caagccatgg gagctgtggt aagcgttgct atggcagcaa tagggccagc
    27841 ggcaatttta ggattggtcg tagcaggtct tggtttagta aacaatcaat ttggtactca
    27901 aattgataaa ttgctaaata tagcaattac tagaggttct caaattatca ctaatttggt
    27961 aaatggcatt acatcacaaa tacctcaatt aattgctagt ggtgctgaac ttatatctaa
    28021 atttgcaaat gctattactg tattgttacc tgttattatt caagcaggtg tccaattaat
    28081 atctagttta gttcaaggtg ttggacaaaa tgctacgagt ttaattagtt cagcaataca
    28141 aattataggg acatttatta gttcaattgc aagtgcatta cctcagcttt tagcagttgg
    28201 tatggaattg atagttaatt tagttcaagg aattgtgcaa aatattccac taattattca
    28261 atatgctcaa caaattatta acaattttgg ggcaagctta caagcaaaca tgccaagcat
    28321 tgttaataat ggcattgcta tcttaactaa tttagtgcaa ggcattatcc aaatgttacc
    28381 cgcagttgta caaattgcaa cccaagtcat cacaggtttt atttccggta tagctcagta
    28441 tttgccacag gtaatccaag gcggcatcca gattataatc atgttagtgc aaggtattat
    28501 tcaaaattta ccacaggtaa tccaagcagc gatgcagatt attcagtcat tgatatctgg
    28561 tttatcacaa gcattaccac aaattatatc tgcagggcta cagttagttg tgcagttggc
    28621 tgttgcaatt attaaagggt tgccacagat tatttcagct ggtattcaat tgattatagg
    28681 acttggcaaa gctatgcttg aggctattcc gaatgctctt tcaggggtat gggaaggcat
    28741 taaaggtgga tttagttcta tgtgggacca aatcacagga aagagctcaa caagtgccgc
    28801 taaagttaca tcagacatgt caacaatgac tatgaatgtt ggtatgcaga cgaccacaat
    28861 ggctaatcaa actaacatag acacaactac tatgctaaat agcattagtc aaaatactgg
    28921 tcttgctaat ttaaatgcga caacaaatgc tactcagatg gcaacaaatg taaatgcaca
    28981 aacagcatct atggcaaatc aaggtttgct gaatgcggca ggatttaatg aagcgttgag
    29041 ttcgaacatg actttagcaa atacaaatgc tactaatcaa gctaatcaaa tgactgctaa
    29101 tgttaataac gctactagcc aaatgagtct ggggtctcta ccgaatttct tggaattatc
    29161 gcaacaggca acaagtaata tgcaaactgc acaagcaaat gctaccactt ccgcgaatgg
    29221 aatgagttct ggagttactg gtgctacagc tggtatggct agtggtgtag ctaataacat
    29281 gtcaggtata actagcactg tatctacagg gatgaatcaa gctgcaaatg ctgcaaaatc
    29341 aggcggtgca aatatatcta acagtgtgaa tagcagtttt acacaagcta aatctacagt
    29401 ttcaagttca atgtcaggaa tgaacagcac tgttactagt ggaatgaata gtattaataa
    29461 tactgtaaaa tcaggtggaa ataatattaa atccaccttt acaagttcct ttaattctgc
    29521 taagtctaca gtttcaaatg ctatgtctag tattcgttcg tcaatttcaa gtggaatgaa
    29581 cagcgctgtg agtgtaacaa gaagtgcaat gaattcggtt gtatctgcaa tgaatgcagc
    29641 agcgagtggt gctagatctg caggttataa tactgggatt ggatttgcaa atggcctagc
    29701 aagcactgca ggaactatta tggctattgc taatagtatt gctgctaatg tcgctgcaac
    29761 aatcagaagc gcaatggata ttcattcgcc ttcgcgagta acgcgagaac ttggtgctta
    29821 tactggtgaa ggttttgcac ttggtatgca ggattggatg gatgatatca atgtaatagg
    29881 taaagagtac gctaatgctg tgactgatca gcgatgggaa acagattcac aattagcgac
    29941 atctgcaaac atatcaagtg caggtgtatc atcatcactt gataatctaa gcgacgaggt
    30001 acgcaaccaa caattgacta gtccaacgtt cgaggttcat aatgaacttg ttggtgatga
    30061 aatttataca actattaaat ctaaagaagc aagagaaagt aataaagata attacttcaa
    30121 ctttgcgaca taagagagga gaaaaatgga tttattagta caaaagagtg atcaatcctt
    30181 tcgattttca gagattggtc tacgaatact tgatgtcgat gaccgttcat catctcttga
    30241 ggttgatagt cgaacagtga aaggtcgaag cggtaaaatc tttgctagtg ctaagtatgg
    30301 cacaaaaaaa gttaaggtta atgggcgctt agttgtagct tctattaaag attttatgac
    30361 aaaaaaagat gatgtgaatg gcttactagt ggatagtgag cctttttaca ttactaaaat
    30421 gtatcctcag aaagaaaatt tatatgattt cgaattgcct gggatgaaag caggtgattt
    30481 agacttatta aatcaatctc atacagcgtg gcactatcgt tggaaagttt atgtctcaag
    30541 tgaacctgag tacacttttg ttggtaagtc tagtgaaggt ctcaaataca actttacaat
    30601 tgcatttgag actgcagaac ttccatttgg tgaaactatt gcacaaaata tagtcattac
    30661 taacggaacg tttaattatt ctggtactgc accttttagc cagttagaat acccatggtc
    30721 tgtagagtta acttcaactg gtggacaaag tagtttttat cttgaaatcg atgggaggcg
    30781 ctgggcttat caatatagtg caccacttaa tcaaggcgac gttattgtaa tcactggtat
    30841 atccacaaca ttgaataaga taaatgtgac tgctagaacc aatcatgaat attttgtttt
    30901 aaagcaaaaa gtcaacaaga ctattctcta cagcacaaat tttcaaggca caatcaagtt
    30961 tttaaacttt aaagaacttt acaagtaagg aagtgataga ttgacaactt ttttagatca
    31021 taaagataat gagtttgaag ctcaagctgt tgttaaaaca actaatgcag ttaacggaga
    31081 aaggtcttta tcaggaacaa tttatactaa cgaagaggta ctaaacaagg tcgataaagg
    31141 ttggaaactt gagtttgatg atgagtatta caagattatt tatgctaaac ctactgatac
    31201 tggtaacaaa attcaagttg aatttgatgc agttcatcaa tttttttatg attttgataa
    31261 atcatcacta cataagcaat taaatgatgg ttcacatact ttccaagcat atttgaactt
    31321 tatttttaat ggcagcgggt atacttacag tttagaagta gttgtcaaat ctttcgaaaa
    31381 gcaatcgttt ggatataaat cgcgacttga tttatttaat gacatcatta agagtgcagg
    31441 agttgaattt tttgttcgtg gcaaggttgt tcgaattcta caaaaaacag gaacagattt
    31501 atcaacaatc gtacgtaaaa atttcaatat gaatgaaatc ataattgaaa agaaaatcaa
    31561 tgattttatt acatatcaga agggttttgg agcgtggact gaccaagagg atcattccaa
    31621 aggaaggctc gaagtagaat atgaaagtcc acttgcaaag gaatatggac gaattgaggg
    31681 tgagcctgtt gttgatgagc ggtacactaa cgttgacaat ttaaaatcag tactaaaaca
    31741 aaatgtagat tcgtcttaca atatcgctgt aaaaattgac attgaagatc ttacaaaagc
    31801 aggttatcag tacactcaac ctattgccgg tgattatatt atggctatca atgagacact
    31861 aggatttaag gaaaaaatcc gaatagtatc gtttgaaagt gagtatgatg taacaggtca
    31921 gctgattaac cataaagtca cttgtaacga tattggtaca atcaaaaaac aaactgtagc
    31981 tgttagccaa ttatcaagta agattaataa taaccaagaa atactagata atgcagtatt
    32041 taaagcaaat caagcactag catcagctga tggtaaaaac actaactact atggtacgga
    32101 gatgccagta gatgatccaa aaggcacgct aagaaagggt gatttgcttt ttttaactgt
    32161 tggcgatact accagaatgt atttctggaa tggtgctgaa tggattatca attcttttag
    32221 cgacgatatt gattttgtaa agaaagaaat tactaataaa atcatagagg ttaatgcagc
    32281 aatgaaactt gctgatgaac agttaactga aaaatataat gcaattgttg ctaaaaatgt
    32341 tagccaagac gaattaatta atcaagcaaa gaatttgtct gattctgcca aaaaagatgc
    32401 tacagaagca ttgacaaacc ttattagtga atctaaaaaa ctgacggata aaatgtctgc
    32461 gttatctaaa acagaagttg agcattattc aacaactaat ctgcaactga cacaacttga
    32521 tggtacagtc aaaggattac aaactagcta cgaggcattg ttaaaaaaag atggagatat
    32581 cactcaaaca ctcgctaatt ataagcagac gattgatcaa aatagtacaa gtattactgc
    32641 taacaaaaag actgttgatg gtacgcttag cagtctacag acccaagtca cacaaacggc
    32701 taatgaaatt acaacgagat tatctcaaac aaatattgaa gcgttgatta caagtaagtc
    32761 tgctgttatc gctgataata aagtcaagga aactgcagat agttttagca gagaaataac
    32821 tcgtgttaat aatactattg ataatttaaa gataggtaca gttaatctac tatctggtac
    32881 taaaaattgg actaaaaatt ggtttaatcg taataactgg caatctgatg acgataactt
    32941 gactattggt aattttagct taacagtgct aaagcgcaaa gcaatgtgga gtggtatatc
    33001 acaattgtat gctgtaaaaa gcggagagaa ttacgttttt agtgcatatg ctaaatcaag
    33061 catagataat gatgttgtta atttatattt gttaccaaat gatcaatcgc aacaggctaa
    33121 gataagtatt agtttcaagt cgatcacttt aaacacggat tggcaacgta tcgttattaa
    33181 atttaacgta acttctgatg gctatatttt accacgcttc gaacgcttta atgaaaatgg
    33241 ttggttgtat attgcaggtt accagcttga acatggaaat gttacaagtg actattcaga
    33301 gtccccagcg gatcaagaag ataaaatgac tgttgaattt aataaaatca acgatacagt
    33361 taatagtcat agtcaactga ttggtaaaca aaacgagagt ctgacagcta caatacaaaa
    33421 agttgataat atacaatcaa cagtatctaa tgtggatgga cgcttgtcac gagttaccca
    33481 aacggctgac ggtatagttg caacagttag tcagcttgat aacaacattt tacctggcac
    33541 aaaaaaattc acaggatggc aaacagaagg tgcagtacta gaggatctaa cacaatcgcc
    33601 ttacccattt gtttttaaaa aatgggtaag cggtaataag gtgtcacctt taatcgaata
    33661 tgatgtcaaa aaagatcaag agtatacgtt tactgcatac atttctagag aacaagcagg
    33721 aaatctatat ttctacttgt atgatctttg gaatcatcat attacaagca caactcctcg
    33781 tgaaactatt attaaagacg tcactcaaac cattagacga tttaaaataa catttattcc
    33841 aactcgagac ggtaaaatca gaccacggtt cgcaatgttg gccagtgatc caggctggtt
    33901 tatggttggt ggttttatgt tatctaaagg tactgctgat ctaccctggt ctgaatccaa
    33961 gcaagatatc aaagataatg ttactgcgat taatacaata gtcgcacaaa cagcgaacag
    34021 ttgggctatt aaaaatttga cttctaacgg tactgtattg agtcagctta atttgactga
    34081 tggtaacgta aaactggaag gtaagttaat acatttgtca ggtcaatcta ttattgataa
    34141 tggcattatt actaatgcga tgataaaaga tttaactgca gataagatta cagcaggaac
    34201 aattgatgct aatcgtataa atgtcattaa tttagatgct agtaatatta ccagcggaag
    34261 aatgtcagcg gacctgatac gtagcggcgt tttaatatcg caaaacggtg caatgttaac
    34321 cgatcttaat acaggacaaa ttgaatttta cacagataat ccagctatca aacgtattac
    34381 tgcaggttat cctaaccaat tcgttaaatt tgcaacaggt aatgttgaag gaaagggcgc
    34441 ggctggcgta acggttatag gttctaatcg atggggaagt gaatcgtcaa acgacggtgg
    34501 tttcgtaggt attcgcgctt ggaatggtaa agacatcgat actcttgatt tagttggcga
    34561 tagtattcac ttagcaagct ctgcgtacac taacgctgat ggttgggaaa tcattacttt
    34621 gccaaatcag ctatcaatag atgcacgtaa cataaatcat cgtgtaacgt ctaagataaa
    34681 agtaggagat atttggctat ggaaaaatac atcaacttat acaagcatga aagatacaat
    34741 taatatgatt attgataact tgcagctact acacaataat aaaacgactg aaaaaggcta
    34801 cagttatact attccaggaa agatttagga gaaaaaatgg aagaaaaaat aaaatcaaat
    34861 ttagctttac aaatagcgca attatcttta gataaagcaa ctctacaagc tcaaaatgag
    34921 caattacaat tacaaattgc tgagttaaat cagcaattag atgagataac taagccagga
    34981 gcaaagtgag gtgagtaaca tgaaacaatg gaacattgtt ggaaaaccat ttccaatcta
    35041 tcaagatgga gtggttgtaa aaacggatgt acgcttacaa ggcgacgatg gtacatattt
    35101 accacaagag ctaattggta atcatgttga taaggataat caatcactga taagacttgt
    35161 acttgaaact tttgcaaaag aaaatgtcgt tgatttcgca atgcttgagt ctgtaaaaga
    35221 tattgagcaa ctaaaagctg acaaagtaac tttaatcaaa aaaattgcag aggctgatga
    35281 ggcaatttct aagatgtcgc aagcaacaaa agatatgcag cttaaagttg ataaagcagt
    35341 tgtcgagtta actgacttag tgacatcaag tgtatctagc ctttccgtgc cgtctacatc
    35401 gatggaatca agctaatgga attaacgttg aataccgtta taacactata ttatttaata
    35461 aaaaaaggag aatttaatat gacatatact tttaaaaaag atcatcttat tgttgacgtt
    35521 tggtaccgtc gtgttgcgaa tggtatttgt gatttcgaag cagtacctaa attatttaat
    35581 ttacgagatg ttgtgcttga gttacttagt caaaaagtta ctgataaaga atcagagtga
    35641 ggtaacttaa tgatagattt tgtgcaagtt ggcgcttttg caggtgctgc tttatctatt
    35701 ttgggagtat ggggatttat tgttaatccc tttaaaaaag caatggaagc taacgagatt
    35761 gctatggctc agctcaagga ctcactcaaa gaactagcat acgagctcaa aaatatagat
    35821 agagaccgag agattacaaa agctgttatt gatcgtcacg aggagcgttt aggtcgtgtc
    35881 gaggatgata tcatcatcaa taaagagcaa atcaaaacat tatttaataa taataaggag
    35941 aaataacatg aacgaattta caacacaaat cattacagta tcagtaccaa tttttggtat
    36001 catcgcaggt attctcacgc acgaagtaaa aaaattactt ataaaaaaag gtggagaaaa
    36061 agctgttaaa atcgcagaaa ttgtcgctcg taatgctgtt gaggcagttg aacaaatatc
    36121 tgcggaagtt ggtggtatca agggacaaga taagctaaca gaagctaaaa ccgctgttat
    36181 tgatggttta tcgcaatata atattaatct tacacagaca caacttgaaa cttttatcga
    36241 agctgcggtt aaacgcatga atgatgagtg gaagaaaggt caataatatg gtaattaata
    36301 ttgagcaagc tatcgcatgg atggcatcta gaaaaggtaa agttacttac tcgatggact
    36361 atcgaaatgg tccttcatct tacgattgct ctagctctgt ctattttgct ctacgctcag
    36421 caggcgcatc agataatggc tgggcagtaa atacagaata tgagcacgat tggttgataa
    36481 aaaacggtta tgttcttatt gcagaaaata ctaattggaa tgcgcaaaga ggtgatattt
    36541 ttatttgggg taaacgtggg gcttcggctg gagcttttgg acatactggt atgtttgttg
    36601 atccagacaa tattattcac tgtaattatg gttataactc aattacagtg aataaccatg
    36661 atgaaatctg gggatataat ggtcaaccgt atgtttacgc ttatcgttac tctgggaaac
    36721 agtcaaatgc taaggttgat aataaatcag ttgtatctaa atttgaaaaa gagttagacg
    36781 ttaacacacc gctaagtaat tcaaatatgc catattacga agcaacaatt tcagaagatt
    36841 attacgttga gtccaagcct gacgtcaata gcactgataa ggagttgctt gttgctggta
    36901 ctagagttcg agtttacgaa aaagtaaaag gctgggcaag gataggcgct ccacagtcta
    36961 atcagtgggt agaagatgct tacttaattg atgcaacaga tatgtaaacc aacagagcga
    37021 tataaatgtc ggtctgttaa tggtgtaagt tacaccgcaa ctaaaaaaac aatttaggag
    37081 gtaaaattcc tttagataag acaaatgccc tcgcttttgc gggggctgtt tattattttg
    37141 aacagatttt tcaggaggta tttaggtgcc ttttactgga tgaaaaatat attttattaa
    37201 aaagtttaat tatataaagg gaaaatcatt gacaataagc tatataaaaa tatataatgt
    37261 atatataaat atcaatggcc tccctcgcat acgcgcagac atgttctgat ggggggtttt
    37321 tttgctaaaa aattgagggt gtttcatggc ggaaaatttt caacacaaaa gttacagaga
    37381 acaagttgaa cttttggaat ctagaggtat tgtctttagt ggaagaaaag ctaaaagtaa
    37441 agcagagtat agtttatctg tgatttctta ctacaaaata aaagaatttg caaagccttt
    37501 tgcaaaaatt caaaaagatg gacaaaagaa aaaaatagat tatcagggta cgaaatttga
    37561 gatagttata tctagatatt atcaggataa gaatttgaga ttaaatttac tccatgcgat
    37621 tgaagatatt gaagtagcaa taaaaacaaa aatcgcctat gttctgggga aaaacggctt
    37681 aggaagctat ggctatctag atttttctaa gtggtgtaac aaagaagagt attgcaaaca
    37741 ctacctttcc tatagtgaaa ataactttaa aaaacagtta aaaagagagc taagaaaagc
    37801 atcatcctct gaattggatg aaaaattaaa actagacaga caaaaatatc ctcctatttg
    37861 gctagcggta aatatgttaa catttgggca gatggttaat ttgctagaat tgatgtcaac
    37921 aactaattta actcaaattt caagcacttt ttcatgtagt aatcctgaat taatttcttg
    37981 gttaaaatgt attaatctag cccgtaatat ttgcgcccac aattcaaata tcatagattt
    38041 taaatttata actgtctcta agttaaaaga agagtggaaa gattttttgt ttgagtataa
    38101 agaaggtgtt ttttcaaaca ggattgctct acctttctta attgtcttgg aaatgatggg
    38161 taaaataaat cctaaatacc attttcgaga tatcattgat tctttgcata aacttattaa
    38221 ggacgaggat acagcaaaat attatggctt tgcatcaatg aacactataa aggaaatcaa
    38281 aaaaaagaaa aactttagag gctgacgctt ggatttctct tccccttatt gacaccctcg
    38341 cataatcatg agataatcac actagcaaga atcgcctgac actagcggtt cttgcttttt
    38401 tatttgccta gaaataatca aaatgttacc atagaataaa aataataagg agccacatta
    38461 tgtcacaaga aaaactaaaa gcaaaagttg aacaagcgtc aggcagtctt aaagaaggtg
    38521 cagggaagct aaccggtgat aaagagttag aagcaaaagg atttgtcgaa aaaacaattg
    38581 ctaaaggcaa agaactagca gatgatgcta aagatgctgt tgaagaggca gtagatgctg
    38641 tcaaagaaaa actgaaataa atattaaccg ctctctaatt gagggcggtt ttttgtgtgt
    38701 ctagagtttg ctttcaatta attgttttaa ttctaataag tcttcttttg tagcattttt
    38761 gttaataaaa ctacgagcag tagatcgttt tgatagatag gttctatgtt ctctattgtt
    38821 ttctgcccac tttttatttg ctttttcttg aggtgttaat tctttatcca tttcaatcat
    38881 ccttattaat aaagtaaaat acaactaaac aaatcgcgaa aataatcaaa tatttcatat
    38941 ttgtcttaga tatgatatac tatcagtagt ggcaaggggc ttgagcccca aactactact
    39001 agaaccttat ttgaatctcc gtggccggtt tttcttttta ggttcttttt ttattgctgt
    39061 gattatgctt gctataccaa ccaacagagt tccgattgaa gtaagcaaat cagcaatttc
    39121 tgatattctc atatcttcct cctttctata tattaattat aatacatgta ctatataaac
    39181 tcaatacttt ttttaaaaat atttatcttt ttttgtctat cagaacagaa aaatttaaaa
    39241 ttgtctattt ttaggatttt tttacgaata gatagagagg aggataaaat atgttataca
    39301 tagatgagtt taaagaagcg attgaaaaag gatatatcag cagtgataca gtgatggttg
    39361 tgcgtaaaaa cggaaagata tttgattatg tgttaccaca cgaaaaagtg agagaagaag
    39421 aggttgtgac agttgagaga gtggaagatg ttatgagaga attggagtaa tgttcgtcgt
    39481 gacataagca gctatcaata gcctgcccca caaaaaagca tattatagtt ggggcgaatt
    39541 tggggcataa gttaaaaact aatctatgtt aacaatgata cgacttttac gtttactact
    39601 cataacgact tatatagata tgttttagac cttataatgc caaccgaaaa aactaactct
    39661 taaagacgct gttaaataat tcgtctagaa aaaccttgtc atatcaatgt ttattgatag
    39721 cgacaaggtt cttttttata tccaatactc aataaacgac tagagagctc aaaggaattt
    39781 agagtttcaa atgtgttaga gcataccctt gaattatctt aactgtgatg ttataattcc
    39841 taaaagataa gttaaaagag aggttgcgaa agagaatgtc agaaaaagct atactattaa
    39901 ttatccttat tgcttttgtt attatcgcta ttataaagat agctctgtta aagttatata
    39961 tgaatacgat aaaacctaaa gtagataatg aaggaaacat cataacaaag cctgtaaagg
    40021 atgatttaac acccgaggaa cgtgaagaac gtgaatttga taaagattgg taaaatcaat
    40081 ataaaagagc aaacaaggtt tgctctttta ttatttttaa aggtaattaa gttgtcctcg
    40141 aaaatcctcc aatgattgat agcctttatc agccattatt tcttttaact ctctgcttac
    40201 acgctgaaaa atttctggtc cttctttttg taaagcggtt ccgatttgga ccatactagc
    40261 tccacataga atgtgttcaa aggcatcacg gccgttttta acaccacctg ttccgataat
    40321 tttaattgaa ggatttaaac gtttgtagaa agcatggacg ttagctaagg cggtaggctt
    40381 gataaaatca ccgccgatac ctccaaagcc atttttaggc tttataacga cagtttcatc
    40441 atcaataaca agtccatttc cgatagaatt aacacagtta ataaaagcta aaggatattt
    40501 gttaaaaatt gtagctgctt gatcaaagtg aaagatatca aagtaaggtg gtaatttgat
    40561 gcccaatggc ttttgataat agctaaagat ttctgaaagg atgaggtcag tcatttcaaa
    40621 atcataagca atttgaggtt ttccaggaac atttgggcac gataaattta actctatcag
    40681 gccattgtaa ctactgtttt caaccttttt aagaataata tgtgtctctt ctggtgataa
    40741 acctactaat gataaaacgt gatttttgct attgtcttgg tcttgtaatt cagtcacgaa
    40801 atcaagataa tagtcaatac ctttattagg gagtcccata gaattaattg atccccagtc
    40861 agtatctgca taacgtggtt gagggtttcc ctctctagct ttaagggtac ctgtttttgt
    40921 aacaaaggaa ccagcttcag aattttcaat agctaataac tcttccttag tcatacaata
    40981 aatacccgct gcattcatca aacaattgtc aaaactaaaa ccagcaattt ccgtttttag
    41041 cgataccata atatcctcct gaagataaat ttttactatt ttatcacttg attaacagag
    41101 cggcaagaaa ataatgatat tggtaataaa atttcgctaa ataacgaacg ttattatttg
    41161 tgtaaacaat aatagtaaag aaataaatgt tgctttctat aatgcataaa aaaataagct
    41221 tatgtaagct tattttttat gttcggcgta agattttctt tagtaattga atactcttat
    41281 attttaatgg atttggatag tagcggaaag tacccatttt acgaacaatg taaccattaa
    41341 aattctgttt aaagcgaagt acgccatctg aaccatcaaa atttccttga atacctaaaa
    41401 aattatagaa agttgattga cgctttagag cctcttgcat aacatattct tgaagaactg
    41461 caggcgcata aaatttatta aattctgtgt atgagccaga gaaaagatag acagtttctt
    41521 tagggctata aataaagagg ctaccagcta aaacaacatc ttgatttcca aatttttgaa
    41581 taaaaggttc gacttctttg cgacgtttat ctaaactagc tagttgattg acaagctcag
    41641 tacgttgacg atggtatttg gcagaatctg tattattttg atggcgatta tcaaggactg
    41701 taatctgttc ttccaatttc tttgcattta attgtaggtt atgatcatat tccctaaagt
    41761 ttagagttgc aatgacaaat tccgccttat ctccgaaact atcataaaaa tcttgataat
    41821 aatctagaga tttatccata tagtcccggc gatcagaagt agaagaagta atatccttaa
    41881 agatatgtag ttcttcccgc ttgaggacac gtatttttat accaaaagac atggctttct
    41941 taaccaaggg gcgtcctttt ttagagaaag atttaagaag attttgagga gtgatacctt
    42001 ctagattttt aacataatgc caatcaggtt caccgccagg ataaccaata tgaagcccat
    42061 cgtggtgata acctatacta gtcaggccat caataagata ggtgttcggt gcacctttag
    42121 gttcaccttc accagtaaac tcttggtagg tatcataagg tttaattaat aattctaaag
    42181 cgccctgaga tttggcatag ttctgtagtt ctttataaaa atgtttcaga tatttagaat
    42241 tggaatgggc tggaccagaa ttgacttcca tatgaagtcc accagtcatt gggatggtat
    42301 aaacaatact gatgatttcc attttcccat cgatttggta gcccatgtag gtaatgtcat
    42361 acccacgttt ttttagaagc tttgccatct caggggtttg cataaacgat tgtaaatcgt
    42421 aatttcctga ataactttcg aactctttag ctgttaattc tttaagcgcc ataaaagtat
    42481 cctttcctaa ctagctatta cgttttttgc gtaaggcata gacataatta gaggctttat
    42541 aaagcaatct gttcactgga atgttaaatt ctccaataaa ttcctcaatg attggttcga
    42601 acttagactt aaaatgatat aaaccaccag ataagtcatt ctcaatcccc cccatatttt
    42661 gccaaatatt tccacgttca aaagcccttt gagctgtttc aaaccaagtg taaataggag
    42721 cagaataaga tttgaagtaa tcgtccatac cagcataaat attttctgat gtattaccaa
    42781 attctagagt gagggttgca gccaatggaa caatgtctcg gtcataggct ttttgagctt
    42841 ctttgagaaa atcaatttct ttatttaatc gatcaatcgt atcttggtgt tgtttaactt
    42901 ttttctcacg attaagttca agtgatttta ttctttcaga ttgagctatc tggcattctt
    42961 tttctatttt ttctaatcgt tttgcaacat ctagcgaagc cattgtgatg tatgagttat
    43021 taggatacgt atcaaggagt ttttggtagt agtcaattcc tctaagattt atccctttac
    43081 ggtcttctgt ttttttcatt aactctgcaa aatcttcaag tagttcatgg ctaccaaatt
    43141 gtatatctac tcccttattt ttggaagttc gaatagcttg acgagttttt ttagacattt
    43201 tatcaagtcc aaattgatta gcatataggt ttgcttgaaa acgtggttgg atagtatgag
    43261 ctaaatctgt tgtccgacca gtccaatcag cacctgcacg ctgtaaatga tcaatggcga
    43321 ttaacccttc tttttcatcg ggcgaatttt caaaatcttt ggcattgacc atttttaaat
    43381 agatgagagg atcacacttg ataaagagag ctctttgttt tttgccaaaa gctttaaggt
    43441 ccttaaggac cttagttaca atatctagat tggagtaatc catgattggt ccacgcggaa
    43501 tatacagcat ggagaagcca agaggaagtg atttgatgag aatactagcg ctggcaattt
    43561 gggtttcacc gtcaaaaaag ccaagtaatt gactacccca gttgtctttt actttgggcc
    43621 aattagaaga ttgaagtagg tttgtttggt tggattcgct cacaaagcga tcgtgttcga
    43681 cagcggtaat ttctctatac atgagtttcc tttcttagtg agagtgtctt tgtttttttc
    43741 gaatagtata ggctaggttg gccaatttat ataataatgg attgattgct agattaaact
    43801 cgccaatata ttcttcaaac attggattga agttactttt aaattttgtt aaaccatcgt
    43861 taagacttcc ctcgacaccg cctagattag cagatacaat accgtttttg aaagcatctt
    43921 cgaacaccct agcattcaag agatactgtg gataaaattt acggaagcta tcatccatac
    43981 cagcatacaa catctccagg acattgccaa aacgaatgga caaaattcct gcaatgacag
    44041 gctcatctgg atatttcttg ctaaactcct gaaattcaag gatatattgt ttaacagaag
    44101 cttcttgttg atttagcctt gtcaggcgac ttttttgatg ggatggagtt tctgataaat
    44161 ctctttggat ttgaagaagt tgttctttaa attgcgctaa tcttttagga agattgactt
    44221 tagccaaata gagataggca tcttctccat aaattgtcat tagttgatgg aaatagtttt
    44281 cattacgtaa agctacacct ttacgattct cggttaatgc tacaaccgtg gcgaatttag
    44341 gtaaatcatc aatgttggca cgataaattt gaacaccgcg atgctttgca tcttttatca
    44401 gacgtttagt gtgttttgga aagttttctt ctatatttgc tttggtatag atgttcgctt
    44461 gaaaacgtgg ttggatggtt tcggaaatgg ctttcgttgg acctatccat tgggcacctg
    44521 ctgacttaag attatcaatt gcctgtctac tttcatctat ctcttcggcg acattctctt
    44581 ctttaaggtg atattgacgt agaagaagtg ctggatcgaa ttttgcaaaa acagcacgtt
    44641 tctttcgtcc aaagtttttt agtgttttta acacaaagcc aactaattct ttattgctgt
    44701 agtctataat aggtccacgt ggaatataaa gcattgtaaa accaagaggt aatgatttaa
    44761 ttaagatgct agctacagca actaattggt catctttata aaagcctaaa cgttcatttt
    44821 gccaatttga ttttacttgt gcccactttg aagagtggag gagattggtt tgagacgatg
    44881 caatagcaaa ggcatcatat tctttttcgg atattccaac ggtaacatga tacatcagtt
    44941 tattacccat tcacgtagtg cgtaggctac gccactttcc tcgtttgatt tggtgatata
    45001 ttttgctatt ttttttagtt caggattgcc gttttgcata acaacaggat ttccaacaac
    45061 ttcaagcata gcacggtcat tttcttcatc accaatagcc attgtttgat ctacagttaa
    45121 tcccattttc tctgcaaggt ggagaagtgc agtgccttta ttaacgtttt tgggtaaaat
    45181 ttctaaataa aaaggagttg attttacaat ggtgtagtta tcgagaactt tttttggaat
    45241 tttagcaatt gctgcatcta agatgtcagg ttgatcaatc atcattagtt taatgatttc
    45301 tttgtctccc atttcttcag gagtacgata gaagagcggc atattaacta aagttgcttc
    45361 gtgtattgta tatttaccaa tatcacgatt cgctgtgtaa attccttctt ttgtaatggc
    45421 gtgcatgtgg acgcctaatt tacgcgctaa cagttcgaaa tccagatagt cttcataggt
    45481 taaggtttct ttgatgatgt catctcctgt ggctgtgtct tgaactagac cgccgttaaa
    45541 ggtgataaca taatctccct cttcattgag tttcaattct tctaaaagat cttggactcc
    45601 gatgattgga cgtcctgtag caataacaat tttaacgtct tttgattttg cttcttgaac
    45661 agctttagcc acttcaggtg taatttcacg cttactattt aatagggtgc cgtcaatatc
    45721 tacggcaaca agtttaattg aagtcatttt atctcccact ctttatttca atatcctaaa
    45781 atactcacct tatatcttac taaaaaataa agggtttgtc atgaatggct attatctttc
    45841 gtgagtgtca aatgctcatt tgtgatataa gattgaaatt cttcttttgt acttgaaaat
    45901 aggctatcta atgtcaacat ttcttttgga aaataaaaac gttgatcacc ataattagat
    45961 ccagttaatg cttttacaat cggtgataag cttgataatt cagcaagttg tccattcttt
    46021 tggataattt gaatttctgt tcgtggattt gaatgttcag gccgataaac atcgtaaggt
    46081 aagtcaaaat tagcatggac accagtgtaa taatcgggat caaaaccaac ctgtgagact
    46141 agttctttca ttttaactag attttcttta tcagcctctt caaaggtaat tgatttaaag
    46201 acttttcgat tgataaaacg attagctaaa tctgccaaaa tgttatcatc agcttgcatc
    46261 caagattgga agtaagtatt cattacccca tcatctaggg ctaaataatc ttgtaaatca
    46321 tattgatccg tgaagaaagg aataagattt ggagaagttt gttcaaaaaa atcgcgatgg
    46381 gtatcaaata agaagcgagc acgttttagt aaattttgta agagtaattc catagcacga
    46441 ctggctggat ggaaataaac ttgcatatac atttggaagc gactaacaat ataatcttcc
    46501 acagcgtgca tcccgttacg agcaaaagca attccagaat cggttggtct aataacgcgt
    46561 aaaattcgtg ttaaatcaaa ttgtccatag ctagcagcag tgtagtaaga atctctgaga
    46621 agatagtcca ttcgatcaca atcaatttga ctagaaatta attgaacaac ttgtttattg
    46681 gggtagctat gattaataac actagctact ttatcaggaa aatcaggagc cacctttcgt
    46741 aaaatagcat taatctctgt ggtaggattg gtgatgattt cctgagtgta agcttcatga
    46801 tcagtattga agagacgctc aaatgtatga gaatatgcgc catgtccgat atcatgtagt
    46861 agggcggcag ccatcgttag cagggactca tttttattcc aaagatcaga ataatgttca
    46921 tcaaagattt ctgtaacttt acgagccagt tcataaactc cgagacaatg tgaaaaacga
    46981 ctatgctctg caccatggaa ggtaaaagaa gtcgttgagg tttgtttaat gcgtcgtaaa
    47041 cgttgaaatt ctttagtgtt aatcaagtca tatatgactt gattatttac atggatataa
    47101 gtgtgaacgg ggtcacgaaa tactttttca ttcatatata tattatagca aaaattgaaa
    47161 cttgagtcag aaattggtag aatagaagga taaaagaagt gagagaagtc atgaaactag
    47221 aaattaacaa taacattcaa attgacaacg aaaccgaaat gattcatgag attcacgatt
    47281 gtcaatttat tgaaaaagga agttacgttt atttgaatta tattaatgct gagggcgaga
    47341 gagtagttat taaggcaaat catgaggaat tgcttatgac gaggttttca aatcctaagt
    47401 ctgtaatgcg ttttcatcgt gaaacgcctg ctttggttaa tatccccact ccacttggta
    47461 ttcaacattt gattacagag acaagtcact atcagtttga tttgtcacag caaaggttgc
    47521 acatcaatta tgtccttaag caaacggaga caggagcctg ttttgcaaat tatgaattaa
    47581 agattcaatg gtattgagat aattaccatt aacaaaagag tagtcattat gaatttcgtc
    47641 tactgaagac gctgggatct ctttatgaga gccatttatt tgataaactg cagtatggtt
    47701 tatttcgact agatgaactt cgttttttcc cttgagatta ttagtgatag caaggataat
    47761 taattgaaaa atggattttt ttctgttaca atgattaaca tatcaaggga gtagcagacg
    47821 gtttaccgtg gcaatgtcgt cattacgaaa gaaatttccg gcgttgtctt aaacagcgag
    47881 acttgttttc ataaaaacag gtctcttttt gtttgtggag agaccaaagg aggaaaggtt
    47941 ttgttaaaag aacagaaaaa atccttgttt tacacacaag gtcaagaaga ggttctaacg
    48001 tctttagaat cttctcgtga aggattatca acaacagaag cgaaaaatcg cttagagatg
    48061 tatggtcgca atgagcttga agaagggaaa aaacgtagtc taatagctaa gttttttgac
    48121 caattcaaag atttaatgat tattatctta ctggttgctg cagctctctc tgttattacg
    48181 gaaggtatgc atggcttaac ggatgcattg attatcttgg cagttgttat cttaaatgca
    48241 gcttttggtg tttatcaaga ggggcaagcc gaagcagcta ttgaagccct caaagatatg
    48301 tcaagcccaa ttgcgcgtgt tcgtcgtgat ggtcatacta ttgaggtgga ttctaaagaa
    48361 ctagttcctg gtgacctcgt tatgttagaa gcaggagatg ttgttcccgc agatttacga
    48421 cttttagaag ccgcgtcttt aaaaattgaa gaagcagctc taactggtga gtcagtacct
    48481 gttgagaaag acatatctca agtagtagca gaggatgcag gtattggtga tcgtgtgaat
    48541 atggcgtatc aaaattcaaa tgtcacgtat ggtcgaggat atggtgttgt tactaacaca
    48601 gggatgtata ctgaagttgg taaaattgct gatatgttgg caaatgctga tgaatctgaa
    48661 acaccattga aacaaagttt agttcaactt tctaaattat tgacttactt aatcgtgatt
    48721 attgcggtta ttactttctt agtggggata tttgtccgta aagaaggttg gattgaagga
    48781 ttgatgactt cagttgcttt agcagttgca gctattccag aagggcttcc tgcaattgtt
    48841 acgattgtct tatcaatggg aacaaaaact cttgctaaac gtaattctat cgttcgtaaa
    48901 ctccctgcag ttgaaacttt aggttctaca gaaatcattg catcggataa gactggtact
    48961 ctgacaatga atcaaatgac tgtcgaaaaa gtgtatacta atggcgttct ccaatcatct
    49021 tcggaagaaa tttcagttga taataacact ttacgtatta tgaatttctc caacgatact
    49081 aagattgatc cttcaggaaa attaatcggt gacccaacag agactgcact ggtacaattt
    49141 ggtttagata agaattttga tgtccgtgaa gttttgaaaa atgaaccacg tgtcgctgag
    49201 ttaccatttg attcagatcg taagttaatg tctacgattc ataaagaatc agatggtcgc
    49261 tactttatcg cagtcaaggg tgctcctgat caattattaa aacgagttac taaaattgaa
    49321 gataatggct tggttcgtga tatcacggct gaagataaag aggctatcct aaataccaat
    49381 aaagaactcg caaaacaagc tcttcgtgtc ttgatgatgg cttataaata tgaaacacaa
    49441 attccaactt tagagactga tattgttgag tcagatttag tattctcagg tttagtggga
    49501 atgattgacc ctgagcgtcc agaagcggca gaagctgttc gagttgctaa agaagcagga
    49561 attcgtccga ttatgattac cggggaccat caagatactg cagaagctat tgcaaagcgt
    49621 ctaggtatta ttgatgcaaa tgacacagaa gatcatgtct ttacgggtgc tgaacttaat
    49681 gaattaagtg acgaagagtt tcaaaaagta tttaaacaat attctgtcta tgcacgtgtt
    49741 tctcctgaac ataaagtgcg tatcgtgaaa gcttggcaaa acgatggcaa agtcgttgcc
    49801 atgactggtg atggtgttaa tgatgctcca tcgctaaaaa cagcagatat tggtattggt
    49861 atgggaataa ctggtacaga agtttccaaa ggtgcttctg atatggttct tgctgatgat
    49921 aattttgcaa ctattattgt ggctgttgag gaaggacgta aagtcttctc aaatatccaa
    49981 aaatctattc aatatcttct ttcagccaac atggcagagg tatttacaat tttctttgca
    50041 actttattag gatgggatgt acttgcaccg gttcaccttc tttggattaa cttggtaaca
    50101 gatactcttc cagctatcgc tcttggagtt gagccagctg aaccaggagt tatgacacat
    50161 aaacctcgtg gtcgccagtc aaacttcttt gatggtggtg ttatgggagc tattatctat
    50221 caaggtattt tacaaactat cttagtactt ggtgtctatg gttgggcatt gatgtatcca
    50281 gaacatgctg gttatcgcat gattcacgca gatgccctta cgatggcctt tgcaacgctt
    50341 ggtttaatcc aattggtaca tgcatttaac gttaaatcag tttaccaatc aatctttaca
    50401 gttggagctt ttaaaaatag aacctttaac tggtctattc cagtagcttt cattcttttg
    50461 atggtgacta ttgttgttcc tggatttaat aaacttttcc atgtaactca tttaagttca
    50521 acacaatggt taactgtagt tatcggaagt ttattgatgg tagtgttaac ggaaattgtt
    50581 aaatttatcc aacgtaaatt aggacaagac gaaaaagcta tttagtttag ctgataaaaa
    50641 aactcactta gaatatctaa gtgagttttt tagcaatcaa atattgtcat agagttgcgt
    50701 aattcgtctt caaaatattt ttttggatag ttttcaaaac tatccaaatg cttaacggca
    50761 ttatgccttt tgctaagatt ccaggttaaa ttagggttat gattgggatt ctgattgaca
    50821 aagtcaatag ttaaattcca ttctctaata tatccaagtg gtctagaatg ataagtagtc
    50881 tctccaattt taacgtcgcc aaatgagcgg tgtgcatgac caaaaacaac atctttgata
    50941 tgatattttt ggaataaatc atgataagcc tgactcccca aaaaagcatt aaaaggagag
    51001 aatctaggat gagtcatcgt gaactgttta tggggaacaa agtgcatagc aataatgata
    51061 tttgaagaat cgacttttgc taatatttcg tctaagcgtt ttaaaattga tgcttggata
    51121 gttacatcat tatttggacg ttttaaacgt ctatcaaacc aaaaagtctt ttttaatttt
    51181 tcaacatcct tgatatctct attatttgaa aagctgtaat catactagcc atgaaaagct
    51241 agtagcattt ttttgtcaaa tcgataggtt tgaaagtcaa ggcgttggat ttctgtttct
    51301 gttaaatcaa gcatatcatg gtttcctaaa ttatagctaa gtttaatatg cttttttaag
    51361 ttattgataa agggaagtgt atctttagta aaatgattag atatatcccc agcgatatgg
    51421 aggtgatcaa ttttttcttt cttaagcaaa ttaattaacg ttttcgtctc aaaggtagaa
    51481 aaatgattga ggtcaatatg taaatcactc atgatagcaa gtttggtcat aagggtattg
    51541 taccatattg tagttcaaag agataaacag taaatttttg aaatagtttt aaagtcattc
    51601 tataatagat atgtcattgt agaatgcgct ttcataattg tgtttagaag ctttatattt
    51661 aaggtgcctt aaggaggtct catgtctaat ttttataagc tcttgaaaga aaaatttcct
    51721 cgaaaagaag atatcgtaac agaaatgatc aatttagaag ctatttgtca attacccaaa
    51781 gggacggaat attttattag tgatttacac ggggaatacg atgctgttga ttatctctta
    51841 cggacaggtg cagggtctat tagagctaaa ttattagact gctttgattg gcagaaaata
    51901 gttgctgttg atttagatga tttttgtata ctgctttatt acccaaagga aaaattagcc
    51961 ttcgataaaa tgaacttgtc agtaagtgct tataaaacga agttatggga aatgattccg
    52021 ttacaaatac aagttttaaa atatttttct agtaaatata caaaatctaa agtacgtaag
    52081 caattgccag gtaagtttgc ttacattatc gaagaacttt tagctgagat tgaccgtaac
    52141 ccagagaaga aaagctattt tgatactatt attgaaaaat tatttgaatt agaccaagta
    52201 gaagatttaa ttatagtatt atcccaaaca attcaagttt taatcattga ccatttacat
    52261 gttgtagggg atatttacga tcgtggtagg tatcctgatc gtatcttgaa taggttaatg
    52321 gcttttccaa atttggatat ccaatggggg aaccatgatg tcacttggat gggagcagca
    52381 tctggttcct acctttgtat ggtaaatgtt atccgtattg ctgcacgtta taataatatt
    52441 actttaattg aagaccgata tggtatcaat ttgcgtcgct tggttgatta ctcacgccgt
    52501 tactatgaac ctctaccttc tttcgccccc atattagatg gagaggaaat gactcatcca
    52561 gatgaattag atttgttaaa tatgatacaa caagcaacag caattttaca gtttaaattg
    52621 gaagcccaat tgattgacag gcgtccagaa tttcaaatgc ataatcgaca acttattaac
    52681 caggtaaatt ataaagattt aagtatatct ataaagggag ttgtgcatca acttaaagat
    52741 tttaatagta gatgcattga ttcgaaaaat cccagtcgat taacaagtga ggaagaagag
    52801 cttttacaac aattgatgat agccttccag acttctgaaa gtttaaagaa acatattgat
    52861 ttcctatttg agaaaggaag tatgtattta acctataatg ataatctttt attccatggt
    52921 tgcataccaa tgcatagtaa tggtgatttt aaaagcttta aaattgcagg aaaaacttat
    52981 ggtggtaggg atttactgga tttatttgaa agtcaaatac gtctagcata tgctagacca
    53041 gagaaacatg atgatttggc gacagatatc gtttggtatc tttggtgtgg tgaaaattct
    53101 tctctttttg gtaagaatgc catgactact tttgagcgct attatgtttc cgacaaagta
    53161 actcatcaag aaagaaaaaa tccctacttt aaactgagag ataaagatga tatttgcact
    53221 gcccttttac aagagtttga tttgcctaag tttggacata ttgtcaatgg tcatacgcca
    53281 gtcaaagaaa aaaatggtga gcaaccgata aaagcaaatg gtaagatgtt agtaattgat
    53341 ggtggctttg ccaaaggtta tcagaaaaat acaggtttag ctggctacac tttgatttat
    53401 aatagctatg ggatacaatt aatttcacac cttcctttta catccattga ggaagtttta
    53461 agtggaacca actatatcat tgatacgaag cgattagtag aagaagccaa ggataggatt
    53521 cttgtaaaag atacaactat tggacaaaag ttaaccaaag agattaaaga cttagatcac
    53581 ctttatcgtc attttcagga atatgatgac tagtgtttaa gtaatttact aattgatatg
    53641 taggatttaa tttttcagtt tcttaccttg tctcttttat taaattgact taaaataata
    53701 ttatggacat aaaggcagaa attcaaaaat tagcaaaaga aattggtatc tctaaaattg
    53761 gttttacgac ggcagataac tttgattatt tggaaaagtc attgcgtgct tctgtggaag
    53821 aggggcgcaa ttcaggattt gaacataaag tcatagagga tcgtatctac cctgaaaggc
    53881 ttttagaatc ggctaaaacg attatttcaa ttggcgttgc ttatcctcac aaacttcctc
    53941 agcaacctca aaaaacacct tataaacgag gaaaaattac acctaattcg tgggggcttg
    54001 attatcatta tgtggtcggc gaaaagttag acagactatc aaaaggcatt gaagagctct
    54061 gtcgagattt tcctttgcaa caaaaagcaa tggtcgatac tggagcttta gttgatactg
    54121 cagttgccca aagagcaggt attggtttta taggaaaaaa tggtctagtt atttctaaag
    54181 aatatggctc ttatatgttt ttgggagaat taattaccaa tttagagatc gagccagaca
    54241 agcctgtcga ttatgattgt ggtgactgtc gtcgttgtct agatgcctgt ccaacttctt
    54301 gtttaattgg tgatggctca atgaatgcca agcgttgtct atcatttcaa acccaagata
    54361 agggaatgat ggacatagag tttcgaaaaa agattaaaac agttatctat ggttgcgata
    54421 tttgccaaat ttgctgtcct tataacaaag gtataaataa tccattggcg acagagattg
    54481 atcctgagtt ggctcaacca gaattaatac catttttgag tttatcaaat ggacaattta
    54541 aagaaaaatt tggtatgatt gctggaagtt ggcgaggtaa aaatatttta cagcgtaatg
    54601 ccattattgc gctagcaaat gctcatgata aaaccgcagt ggttaaatta atagagatta
    54661 ttgataaaaa taataaccct attcatactg caacagctat atgggcattg ggagaaattg
    54721 tcaaaaaacc aaacgatgag atactggagt ttatgagtaa tttaacctta aaagatgaag
    54781 atagccgaaa agagttggaa ttaatccgtc ataaatggca attttaagcc ttttatggta
    54841 tttttaagaa ccttatgata aaataaaaca gattaacaaa gattggagaa atctcatgga
    54901 agtggctgaa attcgccaaa aaatagtaga aaataaagag aagttgacta gcttcaggag
    54961 gtctctttga cttagatcgt ttggaagagg agattgctct tcttgaaaat caaatgactg
    55021 agccagactt ttggaatgac aatattgcag cgcaaaagac atctcaagaa ctgaatgagt
    55081 taaaaggtaa gtatgatact ttccataata tgcaagaatt atctgacgaa acagaactct
    55141 tacttgagat gcttgacgaa gatgattctt tgaaagaaga attagaagaa aatctaatgc
    55201 agctagataa gataatgggt gcttatgaaa tgacgcttct cttatctgag ccttatgacc
    55261 acaataatgc tatcttagaa atccatcctg gttcaggagg aactgaagcg caagattggg
    55321 gagatttact cttacgaatg tatacccgtt ttggcaatgc taatggtttc aaagtagaag
    55381 tattggatta tcaagcaggg gatgaagctg gtattaaatc ggttaccctc tcttttgaag
    55441 gtcccaatgc ttatggtttg ttgaaatcag aaatgggagt tcatcgcctc gttcgtatat
    55501 caccttttga ttcggctaaa cgtcgtcata cctcatttgc ttcagttgaa gtgatgccag
    55561 agttagatga tacgattgaa gtagaagtac gtgatgatga cattaaaatg gatactttcc
    55621 gttccggagg agctggtggt cagaacgtca ataaagtatc aacaggtgtt cgtttgacgc
    55681 atattccaac tggaattgtg gtatcgtcta ccgttgaccg tactcaatat gggaaccgcg
    55741 atcgcgctat gaagatgttg caggctaagc tatatcagtt agaacaagaa aagaaagcac
    55801 aagaagtaga tgctctcaag ggtgataaaa aagaaatcac ttggggaagt cagattcgtt
    55861 cttatgtttt caccccttat acgatggtga aggatcatcg tactaacttt gagcttgctc
    55921 aggtagataa ggttatggat ggagaaatca atggctttat tgatgcctac cttaagtggc
    55981 gcattgaaga ctaaaaataa tgaaggagaa acatggcact aatagaaatg tctggtgtta
    56041 ctaaaaagta tcgccgttca acaactgcct tgagaaactt aaatttatca attcagcaag
    56101 gtgagtttgt gtatttagtt ggtccgtcag gtgctggtaa atctagcttg attcgcctcc
    56161 tataccgtga agaaaaatta agttcaggta gattaaaggt aggggaattc aatctcaata
    56221 aactcaaacg tcgtcaaatt cctattttac gtcgtagtat tggagttgtc ttccaagatt
    56281 ataaattact accaacaaaa acagtttatg agaatgttgc gtttgcgatg caagttattg
    56341 gagctaagcg ccgtcatatt aaaaaacgcg tgccagaagt tttagaatta gtaggtttaa
    56401 aacacaagat gcgttctttt ccaacacagt tatctggtgg agaacaacaa cgtgtggcaa
    56461 ttgcacgtgc gattgttaat aatccaaaat tattaattgc agatgaacct actggtaatt
    56521 tggatccaga aatcgcttgg gaaatcatgc atctattgga acgtattaac cttcaaggaa
    56581 cgactgtttt gatggcaaca cacaatagtc aaattgttaa cacacttcgt catcgtgtca
    56641 ttgagattga agcaggtagc gttatccgag atgaagagaa gggagaatat ggttatcatg
    56701 attaatttct ttagacattt ctgggaatcg cttaaaaact taaaacgtaa cttttggatg
    56761 acttttgctt ctgtaacatc agttaccata acattacttc ttgtaggctt gttttcatca
    56821 gttttactga atgttgaaaa gttaacaact gatgtttcag gtaattttac tatcagtgcc
    56881 tttttaaatg ttgactcaac ggatgctcaa aaacaagtca aagataagga cggaaagcta
    56941 aaagataatc ctgattatca taaagtttat gacaagatta aacgtatttc tggtgtagag
    57001 aaagtcactt attcaagtaa agctgaacaa ttaaaagaag ttcaaaaaga gtatggttca
    57061 gatgttattg atgataccta caaagatgct ttgttagatg tttacgtagt tgggacaagt
    57121 tctgcaaaag tttctaagag tgtcagtgaa gcaattggac gcattgaagg tgttgattat
    57181 actaaagaac ctattgattc aacaaaacta tctaatctca cagataacat ccgtatatgg
    57241 ggatttggtg gtgttgcctt attaattgtc cttgctatct ttttgatttc aaatactatt
    57301 cgtatgtcga ttatgtcacg ccgtacagat attgaaatca tgagattagt tggcgctaaa
    57361 aattcttata ttcgagaacc gttctttttt gaaggtgctt gggtagggat tttaggggct
    57421 atcgttccat cgctgatttt ctattttggt tatcaatttg tgtttaacaa gtttaatcca
    57481 aaatttgaga caagccatgt ttcattgtat cctatggata ttatggttcc agcaattatt
    57541 ggtggtatgg tgattattgg tattattatt ggctcacttg gatctgtcct atcaatgaga
    57601 cgttacctta agatttaata aaaagagatg aaatc
//
LOCUS       DATRUK010000004        57600 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.004, whole genome shotgun sequence.
ACCESSION   DATRUK010000004 DATRUK010000000
VERSION     DATRUK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 57600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57600
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.004"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(86..2914)
                     /gene="uvrA"
                     /locus_tag="VBE34_000186"
     CDS             complement(86..2914)
                     /gene="uvrA"
                     /locus_tag="VBE34_000186"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEO7262943.1"
                     /translation="MQDKLMIRGARAHNLKNISVDIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSVEQIVDKVLALPERTKMQILAPIIRRKK
                     GQHKSTFEKIQKDGYVRVRIDGDIHDVTEVPELSKSKMHNIDIVVDRLINKEGIRSRL
                     FDSVEAALRLSDGYVVIDTMDGNELLFSEHYSCPECGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDIDLVIPDRSKTLREGALVPWNPISSNYYPTMLEQAMTQFGVDVDTP
                     FEKLSKAEQDLALYGSGEREFHFHYINDFGGERNIDLPFEGVVNNINRRYHETNSDYT
                     RNVMREYMNELKCNTCHGYRLNDQALCVRVGGEEGLNIGQVSDLSIADHLELLETLRL
                     SSNEQLIARPIIKEIHDRLSFLNNVGLNYLNLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYVLDEPSIGLHQRDNDRLIDSLKKMRDLGNTLIVVEHDEDTMMAADWLIDVGPGA
                     GAFGGEIVASGTPKQVAKNTKSITGQYLSGKKVIPVPSERRVGNGRFLEIKGAAENNL
                     QNLDVKFPLGKFIAVTGVSGSGKSTLINSILKKAVAQKLNRNSDKPGKYVSLEGIEYV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     ESCSGDGIIKIEMHFLPDVYVPCEVCHGTRYNSETLEVHYKEKNIAQILDMTVNDAVT
                     FFAAIPKIARKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHADDIARLLKVLDRFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGIGGGQ
                     IVAIGTPEEVAENPKSYTGYYLKEKLAR"
     gene            complement(3046..3717)
                     /locus_tag="VBE34_000187"
     CDS             complement(3046..3717)
                     /locus_tag="VBE34_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HEO7262944.1"
                     /translation="MTLQDLTKKNQEFVHIATNQLLADGKSDAEIKAILEEHLPEIID
                     NQKKGITARSLLGAPTTWAASFTERPEDKARVSVQKNTNPWLMWLDTSLLFLGLVTAL
                     NGLMLLFGQSNVNTGLISILTLGFGGGAAMYVTYYYIYRHMGKPKSERPGWLKSFAVL
                     ALVMLVWFALFAVVPLLPATINPKLPEVVLFIIALASFGLRFYLQRKYNIQSSMAPVA
                     PQQRR"
     gene            complement(3742..4686)
                     /locus_tag="VBE34_000188"
     CDS             complement(3742..4686)
                     /locus_tag="VBE34_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO7262945.1"
                     /translation="MKQMFLSTAIEFKEIETFEPGAWINLVNPSQEESEQVADQFGID
                     IDDLRAPLDVEETSRISVEDDYTLVIVDVPTYEERNNKSYYMTIPMGIIVTDNAVITT
                     CLEHLTLFDHFYRRRVKNFYTFMKTRFVFQLLYRNAELYLQALRTIDRQSDKIEAQLE
                     SATRNEQLIDMMELEKSIVYLKASLKFNERIVKKLTSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMANIYENVLNAMTETTASIIGNNQNTIMKTLALVTMTLDIPTVIFSAYGMNF
                     KNNWMPLNGLAHGFIYVVLLAFLMSSFVVFYFIRKKWF"
     gene            complement(4856..5095)
                     /locus_tag="VBE34_000189"
     CDS             complement(4856..5095)
                     /locus_tag="VBE34_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEO7262946.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(5140..5631)
                     /locus_tag="VBE34_000190"
     CDS             complement(5140..5631)
                     /locus_tag="VBE34_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO7262947.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFSLAVNRNFKNQSGER
                     EADFINCVIWRQQAENLANWAKKGALVGITGRIQTRNYENQQGQRVYVTEVVAESFQL
                     LESRATREGGSPNSYNNGGYNNAPSNNSYSASSQQTPNFSRDESPFGNSNPMDISDDD
                     LPF"
     gene            complement(5643..5930)
                     /locus_tag="VBE34_000191"
     CDS             complement(5643..5930)
                     /locus_tag="VBE34_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEO7262948.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILSDNGATVVESKDWE
                     KRRLAYEIQDFTEGLYHIVNVEAEDAVALNEFDRLSKINGDILRHMIVKVD"
     gene            7024..8148
                     /gene="mutY"
                     /locus_tag="VBE34_000192"
     CDS             7024..8148
                     /gene="mutY"
                     /locus_tag="VBE34_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEO7262949.1"
                     /translation="MWPEDRIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YSKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFQREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            8325..8918
                     /locus_tag="VBE34_000193"
     CDS             8325..8918
                     /locus_tag="VBE34_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7262950.1"
                     /translation="MEIGQQIIRYRKQQALSQEELAEKVYVSRQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFERDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQTYKEILAVSSGKLLDEIEKREE
                     RAKLPYQKPLIVTVFFLITVATFFASRFIFTWLFHLS"
     gene            complement(8964..9278)
                     /gene="trxA"
                     /locus_tag="VBE34_000194"
     CDS             complement(8964..9278)
                     /gene="trxA"
                     /locus_tag="VBE34_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEO7262951.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(9359..9859)
                     /locus_tag="VBE34_000195"
     CDS             complement(9359..9859)
                     /locus_tag="VBE34_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO7262952.1"
                     /translation="MESYEQFYAKLSQPFRKSPQLIILLNFLLKIVTGMMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRLDFPRPYEKWNIKPLIYKDTEGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLIL
                     GLCLFI"
     gene            complement(9863..12202)
                     /locus_tag="VBE34_000196"
     CDS             complement(9863..12202)
                     /locus_tag="VBE34_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEO7262953.1"
                     /translation="MNNKILEQLEFNKVKELILPYLKTEQSQEELSELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEESRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPEISNDSTLALINVRHPLLSNPVANDLHFDQDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADHNSLVLFDELGAGTDPQEGASLAMAILEHLRLSNIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQ
                     KVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDNFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG"
     gene            complement(12287..12829)
                     /locus_tag="VBE34_000197"
     CDS             complement(12287..12829)
                     /locus_tag="VBE34_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEO7262954.1"
                     /translation="MLSLLLLIIVIWHFYIGYSRGIFLQVFYVLMSMVSLMIASQFYQ
                     ELASQITLWVPYSNPVQGVEVYFFKDISKFQLSHVYYAGVAFVFIYSLSYLVGRLLGV
                     LLHLAPVEHFDSLQNNIISGFLAVLVCLLFMSMCLTILATVPMSFVQEKLWNSLFVRF
                     LINDLPFFSQFLVRTWAVGL"
     gene            complement(12832..13143)
                     /locus_tag="VBE34_000198"
     CDS             complement(12832..13143)
                     /locus_tag="VBE34_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262955.1"
                     /translation="METKINNSQKNRYKFVFGDKPLTLTTDKDNLFMEEIERVATEKY
                     EAIKEKLPNADNETIAILMAINALSVQLSREIDIEKMEDELNKLRSKTISDIKEKVSE
                     D"
     gene            13254..14147
                     /locus_tag="VBE34_000199"
     CDS             13254..14147
                     /locus_tag="VBE34_000199"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEO7262956.1"
                     /translation="MNTIVMQADKKLQEKIRTDLAQHHISNNNPYVVFSAKISGATVL
                     LYTSGKLVFQGSNASHIAQKYGFIEQKESCSSESQDIPIIGTDEVGNGSYFGGLAVVA
                     SFVTPKDHAYLKKLGVGDSKTLTDQKIKQIAPLLEKAIPHKALLLSPQKYNQVVSPNN
                     KHNAVSVKVALHNQAIFLLLQDGFEPEKIVIDAFTSSKNYQNYLKNEKNQFKQTITLE
                     EKAENKYLAVAVSSIIARNLFLENLNKLSDDVGYKLPSGAGHQSDKVASQLLKAYGIS
                     SLEHCAKLHFANTKKAQALLK"
     gene            14163..14756
                     /gene="lepB"
                     /locus_tag="VBE34_000200"
     CDS             14163..14756
                     /gene="lepB"
                     /locus_tag="VBE34_000200"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO7262957.1"
                     /translation="MKEFIKEWGVFILILSLFLLSRIFLWQFVKVDGHSMDPTLADKE
                     QLVVLKQTKINRFDIVVANEEEGGQKKKIVKRVIGMPGDVIKYKNDTLTINNKKTEEP
                     YLKEYTKLFKKDKLQEKYSYNPLFQDLAQSSTAFTTDSNGSSEFTTVVPKGHYYLVGD
                     DRIVSKDSRAVGSFKKSTIVGEVKFRFWPIRRFGTIN"
     gene            14885..17305
                     /locus_tag="VBE34_000201"
     CDS             14885..17305
                     /locus_tag="VBE34_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEO7262958.1"
                     /translation="MEVFFTGTIERIIFENASNFFKILLLEIEDTDSDFDDVEVIITG
                     TMADVIEGEEYTFWGTLTQHPKYGEQLQSVRYERAKPTSGGLVKYFSSEQFKGIGKKT
                     AQRIVELYGDNTIDKILESPEQLSTISGLSKINREAFIAKLKLNYGTEQVLAKLAEYG
                     LSNRAAIQIFDHYKEESLEVINENPYQLVEDIQGIGFKIADQLAEQVGIESDSPKRFR
                     AAIIHTLVESSMEQGDTYIEARTLLEKTITLLEEARQIELDPSIVAKELTNLIAEDKV
                     QHIGTKIFSNTLFFAEEGIKKNLQRILNQPLDKQLNHKDIDREIRDIQKSLNIHYDNI
                     QEKAIREALLSKVFILTGGPGTGKTTVINGIIEAYSELHHIDLNKNDIPIVLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDSDYQSLDDYLDCSLIIIDEFSMVDTWLANQLFD
                     ALDSHTQVIIVGDSDQLPSVGPGQVLADLLNINALPHVKLEKIFRQSEESTIVTLANQ
                     MRQGFLPEDFTAKKADRSYFEASANIIPNMISKIVQSALKSGIEAHEIQILAPMYRGQ
                     AGINNLNLIMQNLLNPLKDNNQFTFNDINFRIGDKVLHLVNDTELNVFNGDIGYITDL
                     IPAKYTESKQDEIYMTFDGQEVIYQRKEWLKITLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRMLQRNLIYTAITRSKSKLILLGEIGAFDFAVKNEGAKRNTYLIERFENKQEIANS
                     QKIEDSSIDQKIDNTIINTSIPKTATPIEQTNLSKITYRLTEENYLTIDPMIGINQQD
                     ISAIFDSK"
     gene            17419..17901
                     /locus_tag="VBE34_000202"
     CDS             17419..17901
                     /locus_tag="VBE34_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262959.1"
                     /translation="MVSYEKVRRSLRTATITIIVLNSLSLVFRLFTGISVQLAKTEIN
                     KGNTGNLPKEHIEAVLSATTPFMLFVTALIVLVNIAIVIFCIKNLRAIKRNQMVNYLP
                     YYLGFAITVGLVILGFLTTKAPWAIAINIVFQAIFGLLYFHAYQKAQKLNERDLEETN
                     "
     gene            complement(17972..19081)
                     /gene="dinB"
                     /locus_tag="VBE34_000203"
     CDS             complement(17972..19081)
                     /gene="dinB"
                     /locus_tag="VBE34_000203"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEO7262960.1"
                     /translation="MGERTMLIFPLINDTSRKIIHIDMDAFFASVEERDNPSLKGKPV
                     IIGSDPRKTGGRGVVSTCNYEARKFGVHSAMSSKEAYERCPQAIFISGNYQKYRQVGM
                     EVRDIFKKYTDLVEPMSIDEAYLDVTENKMGIKSAVKLAKMIQYDIWNDVHLTCSAGI
                     SYNKFLAKLASDFEKPKGLTFILPDQAQDFLKPLPIEKFHGVGKRSVEKLHALGVYTG
                     EDLLSLSEISLIDMFGRFGYDLYRKARGINASPVKPNRVRKSIGSEKTYGKLLYNEAD
                     IKAEISKNVQRVVASLEKNKKVGKTIVLKVRYADFETLTKRMTLEEYTQDFQIIDQVA
                     KAIFDTLEESVFGIRLLGVTVTTLENEHEAIYLDF"
     gene            19251..21563
                     /gene="pflB"
                     /locus_tag="VBE34_000204"
     CDS             19251..21563
                     /gene="pflB"
                     /locus_tag="VBE34_000204"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEO7262961.1"
                     /translation="MATVKTNTDIFEQAWEGFKGVDWKEKASIARFVQANYAPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEETRFPMDTRVASISELPAGFIDKDNELIFGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYATTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLAVYGADYLMQEKVNDWNALNDIDEESIRLREEI
                     NLQYQALGEVVKLGDLYGVDVRKPAMNTKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFARTKAYDALYSGDPTFITTSMA
                     GMGADGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWSDQLPYAFRRYCMSMSHKHSSI
                     QYEGVSTMAKEGYGEMSCISCCVSPLDPENEDKRHNLQYFGARVNVMKALLTGLNGGY
                     DDVHKDYKVFDIDPIRDEVLNFDTVKANFEKSLDWLTDTYVDAMNIIHYMTDKYNYEA
                     VQMAFLPSHVRANMGFGICGFANTVDSLSAIKYATVKPIRDEDGYIYDYETVGDFPRY
                     GEDDDRVDSIAEWLLEAFHGRLAKHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKG
                     VYLNEDGSVNLSKVEFFSPGANPSNKAKGGWLQNLNSLSKLDFAHANDGISLTTQVSP
                     RALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLKDVYDKIMNGEDVIVRISGYCV
                     NTKYLTPEQKTELTQRVFHEVLSMDDALTN"
     gene            21664..22037
                     /locus_tag="VBE34_000205"
                     /pseudo
     CDS             21664..22037
                     /locus_tag="VBE34_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
     gene            complement(22072..23001)
                     /locus_tag="VBE34_000206"
     CDS             complement(22072..23001)
                     /locus_tag="VBE34_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEO7262962.1"
                     /translation="MIKTNTVLELIKEQIKDNLYHGASLAIYENGEWHEHYLGTIDGN
                     EKVKAGLVYDLASVSKVVGVGTLLAKLVYQGTIDIDKPLRYYYPTFHHQTLTVRQLAT
                     HSSGIDPFIPNRDQLNATQLKDAINHIKVLEDKSFKYTDINFLLLGFMLEEVLGDSLD
                     KLFKRYIFTPFQMKETSFGPRVEAVPTVAGINDGIVHDPKAKVLGKHTGSAGLFSTID
                     DLQRFSIHYLKDDFAKPLWNNYSLSKSRSLAWDIDKDWINHTGYTGPFIALNYQKQAA
                     AIFLTNRTFSYDDRPLWIKKRRHVQEAITSYFE"
     gene            complement(22998..23753)
                     /locus_tag="VBE34_000207"
     CDS             complement(22998..23753)
                     /locus_tag="VBE34_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEO7262963.1"
                     /translation="MTLFHSLTFKHPVLRVNNRDLNIAFYQESLGFKLISEENAIAVF
                     SAWQNKEASFIIEESPTYRTRAVNGTKKLAKIIVKSQDAKDIEKLLANGAQAIQVYQG
                     QNGYAYETVSPEGDLFLLHAEDDLSQLVAIERPELEKKDDTTGLSNFAFQSISLNVPD
                     AVKAEAFYDKVFAGKFPINLSFKEAQGQDLQIAPNETWDIEILECCVNEDTNLNDLKS
                     TFESWGLDVYLDSKEKILVISDTSNIEIWISKE"
     gene            23878..24774
                     /locus_tag="VBE34_000208"
     CDS             23878..24774
                     /locus_tag="VBE34_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEO7262964.1"
                     /translation="MNETLLLHGIQLILIIAMIITFYQIVRHIRSQKINPFKRFFTGL
                     WIGFVTDALDTLGIGSFATTTTFFKLTKLVEDDRKIPATMTAAHVLPVLLQSLCFIFV
                     VKVEALTLITMAGAAFIGAFVGAKMTKNWHAPTVQRILGTLLITAAIIMLYRMITNPG
                     AGISDSVHGLHGIWLFVGIVFNFIIGVLMTMGLGNYAPELIFFSLMGLSPAVAMPVMM
                     LDAAMIMTASSTQFIKSGRVNWNGFAGLVTGGILGVIVAVLFLTNLDLNSLKTLVVGI
                     VLFTGAMLIRSSFKKTMPKSHH"
     gene            complement(24853..25701)
                     /locus_tag="VBE34_000209"
     CDS             complement(24853..25701)
                     /locus_tag="VBE34_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO7262965.1"
                     /translation="MIEITWTVKYITEFIATAFLIILGNGAVANVDLKGTKGNNSGWI
                     IIAIGYGLGVMMPALMFGNVSGNHINPAFTLGLAFSGLFPWAHVGQYILAQILGAIFG
                     QLVVVMVYQPYFVKTENPNHVLGSFSTISALDDGQKSSRKAAYINGFLNEFVGSFVLF
                     FGALALTKNYFGVELVGKLVQAGYDQTTAATRISPYVTGSLAVAHLGIGFLVMTLVAS
                     LGGSTGPALNPARDLGPRIVHRLLPKQILGQAKEDSKWWYAWVPVLAPIVASILAVAL
                     FKLLYL"
     gene            complement(25869..26321)
                     /locus_tag="VBE34_000210"
     CDS             complement(25869..26321)
                     /locus_tag="VBE34_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO7262966.1"
                     /translation="MTQKYERILIAIDGSYESELAVEKGINVALRNDAELLLTHVIDA
                     HAYQSEGVFSDYVFDRQEQESADVLAYFEKLAHSKGLTKIKKITEIGNPKTLLAKDIP
                     IREKADLIMVGATGLNTFESLLIGSTSEYILRHSKVDMLVVRDSKKTL"
     gene            complement(26339..27541)
                     /locus_tag="VBE34_000211"
     CDS             complement(26339..27541)
                     /locus_tag="VBE34_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7262967.1"
                     /translation="MEDKLFNKHFIGITILNFIVYMVYYLFTVIIAFIATKELGVSTS
                     QAGLATGIYIVGTLIARLIFGKQLEVLGRKLVLRGGAIFYLLTTLAYFYMPSIGVMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPADKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HINFKMVIVLCSILIAIVVLGAFVFPVKNITLNPEQLAKSKSWTIDSFIEKKAIFITI
                     IAFLMGISYASVLGFQKLYTTEINLMTVGAYFFIVYALVITLTRPSMGRLMDAKGDKW
                     VLYPSYLFLTLGLALLGSAMGSVTYLLSGALIGFGYGTFMSCGQAASIKGVEEHRFNT
                     AMSTYMIGLDLGLGAGPYILGLVKDGFLGAGVQSFRELFWIAAIIPVVCGILYFLKSS
                     RQVETKTI"
     gene            27697..28356
                     /locus_tag="VBE34_000212"
     CDS             27697..28356
                     /locus_tag="VBE34_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO7262968.1"
                     /translation="MNHQILQNYINSHNLPIIEKDYHKYLTFESLEEDYTYILKDGIV
                     KQSVLSKYGTEFNLRYVTGLEITSILNTDYSQHMGEPYNVRIESETAHFYKVRRSTFL
                     KDINNDIELQGYVKDFYHNRLEKSMKKMQCMLTNGRIGAISTQLYDLSKMFGEERDNG
                     DIYINFVITNEELGKFCGISTGSSVSRILKQLKDDHIIRIEKQHIIITNVEKLKDHIV
                     F"
     gene            28380..30665
                     /locus_tag="VBE34_000213"
     CDS             28380..30665
                     /locus_tag="VBE34_000213"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEO7262969.1"
                     /translation="MRYNQFSYIPTKPNEAFEELKGLGFPLNKKNSDKANLEAFLRHS
                     FLNQTDTDYALSLLIVDAKTDALTFFKSNSDLTLENLQWIYLQLLGFIPFVDFKDPKA
                     FLQDINFPVSYDNIFQSLHHLLACRGKSGNTLIDQLVADGLLHADNHYHFFNGKSLAT
                     FNTNQLIREVVYVETSLDTMSSGEHDLVKVNIIRPTTEHTIPTMMTASPYHQGINDPA
                     ADQKTYQMEGALAVKQPKHIQVDTKPFKEEVKHPSKLPISPATESFTHIDSYSLNDYF
                     LSRGFANIYVSGVGTAGSTGFMTSGDYQQIQSFKAVIDWLNGKVTAFTSHKRDKQVKA
                     NWSNGLVATTGKSYLGTMSTGLATTGVEGLKVIIAEAAISTWYDYYRENGLVCSPGGY
                     PGEDLDVLTELTYSRNLLAGDYIKNNDCYQALLNEQSKAIDRQSGDYNQYWHDRNYLT
                     HVNNVKSRVVYTHGLQDWNVKPRHVYKVFNALPQTIKKHLFLHQGQHVYMHNWQSIDF
                     RESMNALLSQELLGIDNHFQLEEVIWQDNTTEQTWQVLDAFGGNHQEQIGLGDSKKLI
                     DNHYDKEAFDTYCKDFNVFKNDLFKGNNKTNQITINLPLKKNYLLNGQCKLHLRVKTS
                     DKKAILSAQILDYGPKKRFKDTPTITFLNSLDNGKNFAREALRELPFTKDHYRVISKG
                     VLNLQNRTDLLTIEAIEPEQWFDIEFSLQPSIYQLSKGDNLRIILYTTDFEHTIRDNA
                     SYSITVDLSQSYLTIPTNQGN"
     gene            30669..31028
                     /locus_tag="VBE34_000214"
     CDS             30669..31028
                     /locus_tag="VBE34_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262970.1"
                     /translation="MKLLTKERFDDSQHFWYQINLLQESNFGAVFDHDNKNIPQVVAT
                     IVDDLQGSGSSNYFWYFGNTTDTSILMIAHLNRKFYIQVNLKNFDFALNLIAINNWKS
                     LLQTQLEALNDTLAIFQ"
     gene            31074..32054
                     /locus_tag="VBE34_000215"
     CDS             31074..32054
                     /locus_tag="VBE34_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO7262971.1"
                     /translation="MSSYWNNYPELKKNIDETNQLIQERIQVRNKDIEAALSQLTAAG
                     GKQLRPAFFYLFSQLGNKENQDTQQLKKIAASLEILHVATLIHDDVIDDSPLRRGNMT
                     IQSKFGKDIAVYTGDLLFTVFFDLILESMADTPFMRINAKSMRKILMGELDQMHLRYN
                     QQQGIHHYLRAISGKTAELFKLASKEGAYFGGAEKEVVRLAGHIGFNIGMTFQILDDI
                     LDYTADKKTFNKPVLEDLAQGIYSLPLLLAIEENPDIFKPILDKKTDMATEDMEKIAY
                     LVVSHRGVDKARHLARKFTEKAISDINKLPQSSAKKQLLQLTNYLLKRKI"
     gene            complement(32139..33887)
                     /gene="cydC"
                     /locus_tag="VBE34_000216"
     CDS             complement(32139..33887)
                     /gene="cydC"
                     /locus_tag="VBE34_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEO7262972.1"
                     /translation="MFKIPLFKELKTDQWIKPFFKQYKVSLVIALFLGFMTFFSASAL
                     MFNSGYLISKSASLPSNILLVYVPIVLTRAFGIGRPVFRYIERLTSHNWVLRMTSQLR
                     LKLYHSLESNAIFMKRDFRLGDVMGLLAEDINYLQNLYLRTIFPTIIAWILYSFIIIA
                     TGFFSLWFALMMLLYLAIMIFLFPLWSILANGARQTREKELKNHLYTDLTDNVLGISD
                     WIFSQRGQEYVALHERSESELMAVQKKIRSFDNRRALIVELVFGFLAILVIIWASNQF
                     IGHRGGEANWIAAFVLTVFPLSEAFAGLSAAAQETNKYSDSIHRLNELSETYFETTQN
                     QLPNKPYDFSVKNLSFQYKPQEKWVLHHLDLDIKEGEKIAILGRSGSGKSTLASLLRG
                     DLKASQGEITLGGADVSIVGDCISNYIGVIQQAPYLFNTTLLNNIRIGNQDASEEDVW
                     KVLERVGLKEMVTDLSDGLYTMVDEAGLRFSGGERHRIALARILLKDVPIVILDEPTV
                     GLDPITEQALLRVFMKELEGKTLVWITHHLKGIEHADRILFIENGQLELEGSPQELSQ
                     SSQRYRQLKAADDGDL"
     gene            complement(33880..35598)
                     /gene="cydD"
                     /locus_tag="VBE34_000217"
     CDS             complement(33880..35598)
                     /gene="cydD"
                     /locus_tag="VBE34_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEO7262973.1"
                     /translation="MLDKAVMRLSGIHKLLGLLAGLDVLQAIFIIGQAYYLSLSITGL
                     WEGQKLSSQTVYVLLFMVSYLGRHVIDYIKNRKLDDFSTAQSSLLRRQLLDKLFDLGS
                     KVVQEQGTGNVVTMALDGVSLVENYLRLVLNKMINMSIIPWIILAYIFYLDIESGAIL
                     LIVFPLIIIFMIILGYAAQAKADKQYESYQVLSNHFLDSLRGIDTLKYFGLSKRYGKS
                     IYQTSESFRKATMSTLKIGILSTFALDFFTTLSIAIVAVFLGLRLLNEQIYLLPALTI
                     LILSPEYFLPVRDFSSDYHATLDGKNAFQAIQKVLNKTGIKGEQLVIDDWSKESRLDL
                     ENIAIAYDQKRVVEDVTLRFRGHQKVALVGVSGSGKSSLINLLSGFLGPDNGSLKVDG
                     REVTNLDQEDWHKQMIYIPQTPYVFEMSLRDNITFYTPNASDEEVVRAIHMVGLDSLL
                     SELPDGLETRIGNGARPLSGGQAQRIALARAFLDQNRRIMVFDEPTAHLDIETELELK
                     EKMLPLMSDRLVIFATHRLHWLNQMDVIVVMEKGRVAEVGSYQELLAKKGYLYQLKHA
                     MGGNDV"
     gene            complement(35598..36617)
                     /gene="cydB"
                     /locus_tag="VBE34_000218"
     CDS             complement(35598..36617)
                     /gene="cydB"
                     /locus_tag="VBE34_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEO7262974.1"
                     /translation="MSALQFFWFFLIGLLFSGFFFLEGFDFGVGMAVQTLTHNEHEKD
                     QVVETIGPVWDGNEVWLLTGGGAMFASFPYWYASLFSGYYLILLTILFGLIIRGVSFE
                     FRHKVPAEKKQFWNWTLTIGSAIVPFFFGIMFISLIQGMPLDASGNLSAQFSDYFNIF
                     SLVGGVAMVLLAYLHGLNYIALKTEGPIRERARNYAQLLYWVLYLGLALFAVLLYFKT
                     DFFSNHPIVTTIMVLVIVVLAVLAHASTFKGAEMTAFLASGLSLVSVVVLLFQGLFPR
                     VMISSISPKYDLLIQNASSTPYTLKVMSIVAITLVPFVLAYTAWAYYIFRKRITLPVI
                     VTGEK"
     gene            complement(36618..38045)
                     /locus_tag="VBE34_000219"
     CDS             complement(36618..38045)
                     /locus_tag="VBE34_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEO7262975.1"
                     /translation="MTIETLARFQFAMTTVFHFFFVPFTIGTCLVVAIMETMYVITKN
                     EEYKKLTKFWGNIMLLSFAVGVVTGIIQEFQFGMNWSDYSRFVGDIFGAPLAIEALLA
                     FFMESTFLGLWMFTWDNKKISKKLHVTFIWLVVFGSLMSAMWILTANSFMQHPVGYEV
                     VNGRAQMTDFLALVKNPQFFYEFTHVIFGAITMGGTVVAGMSAFRLLKSEQLKDTTVE
                     LYKKSVRIGLVVALLGSISVMGVGDLQMKALIHDQPMKFAAMEGDYEDSGDPAAWSVV
                     AWANEAEHKQVFGIKIPYMLSILSYGKPSGSVKGMDTANKELVAKYGKDNYYPMVNLL
                     FYGFRTMAAMGTAIMGVSVLGLFLTRKKKPILYKHKWMLWIVALTTFAPFLANTFGWI
                     VTEQGRYPWTVYGLFKIKDSVSPNVSVASLFVSNTVYFLLFGGLAVMMISLTIRELKK
                     GPEYEDEHGHHGAYTSIDPFEKGAY"
     gene            complement(38148..39356)
                     /locus_tag="VBE34_000220"
     CDS             complement(38148..39356)
                     /locus_tag="VBE34_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7262976.1"
                     /translation="MKEILVLGAGYAGLKAVRNLQKQSGDFHITLVDMNDYHYEATEL
                     HEVAAGSQPKEKITFPIKDVINTNKVNFMQDEVLRVDAENKTVTVKNNGELHYDYVVV
                     ALGFVSETFGIKGAMENALQMTNISQAENIHNHIVNTMKLYRETKDENLLKLLVCGAG
                     FTGIELAGAMVDERPKYAALAGVKPEQIEIICVEAATRILPMFDDELAQYGVNLIKDL
                     GINLMLGSMIKEIKPGEVVYGTSKEDEELKSITAGTIIWTTGVSGSPVMGESGFDQRR
                     GRVMVNSDLRDPKYDNVYVIGDVSAFMDTESGRPFPTTAQIATRMGAHVAKNLLHQIK
                     GEATEDFSYSPQGTVASVGNTHGLGVVGKTKIKKYPASVMKKIIMNKSLVDMGGLKEL
                     LAKGRFDLYH"
     gene            complement(39369..40268)
                     /locus_tag="VBE34_000221"
     CDS             complement(39369..40268)
                     /locus_tag="VBE34_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEO7262977.1"
                     /translation="MTLPIFLELVEMKAKTASVLPFLIGLCFSAYYYNSVHPVYVGLF
                     FVAMFLFNMFVDIWNNYNDYRNAVDLDYKNDTNIIGRENLSLRQIEVIMASLVITSSM
                     IGLVLVSQVGLPLLWMGLFCFGIGVLYSFGPRPLSSLPLGEVFSGLTMGFMISLICVY
                     LNTYQNFSWDILNLSKIFLISLPNTLWIANLMLANNLCDKEEDEKNHRYTLVHYTGIR
                     GGLLLFAISNSIALLAIVFEFLFGLAPVTVLLSLLLIPFIYKQTKLLWQKQVKRETFV
                     CAVRILALGSATQVLTYLIGIIF"
     gene            complement(40677..41282)
                     /locus_tag="VBE34_000222"
     CDS             complement(40677..41282)
                     /locus_tag="VBE34_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7262978.1"
                     /translation="MNKLITEKKVNNVSTANYLKLGLVSAMFAGGAFVALGSTQGVSA
                     STFTAPQATPPKAERQLTDSELYERAQKQVLPRYIQGSLSGILNQHSTLYKQQNAAVT
                     PQVSSPKSERQLTDSELYESAQKQVLPKYIQGSLSGILNQHSTLYKQQNAAVTPQVSS
                     PKSERQLTDSEIYERAQKQVLPNYIQGSLSGILNQHSTLNA"
     gene            42165..42464
                     /locus_tag="VBE34_000223"
     CDS             42165..42464
                     /locus_tag="VBE34_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEO7262979.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQTQADLAASQFTGKSAQELVTVTFT
                     GDKKLISIDYKEAVVDPEDIETLQDMTTQAINDALSQVDDATKKIMGAFAGKMPF"
     gene            42624..43814
                     /locus_tag="VBE34_000224"
     CDS             42624..43814
                     /locus_tag="VBE34_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7262980.1"
                     /translation="MVMDSLIIKQLIKSTFDIPLQVTYPNGNIETYNGSNPHVKLKLN
                     KNFSVSELSKDPSIVLGEAVMDGDIEIYGSIQELILSAYRCGDSFLRNSKFSKLIPKQ
                     FHDKKHSKSDIQKHYDIGNDFYKLWLDDTMTYSCAYFKHENDSLEQAQLNKVHHILNK
                     LNAQPGGKLLDIGCGWGTLIITAAKEYGLNATGITLSEEQASFITKRIKEEGLENKVT
                     VLIKDYRDIRETYDYITSVGMFEHVGKENLSQYFQTISKRLNINGLALIHGITGQVGG
                     NHGSGTNSWINKYIFPGGYIPRLTENLNHIASAGLQIADLEPLRRHYQKTLELWTKNF
                     HNALPEVQKTHDKRFINMWDLYLQSCAASFESGNIDIFQYLLSKGVSKDTMPMTRDYM
                     YSAN"
     gene            complement(43884..44609)
                     /locus_tag="VBE34_000225"
                     /pseudo
     CDS             complement(43884..44609)
                     /locus_tag="VBE34_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(44736..45323)
                     /locus_tag="VBE34_000226"
     CDS             complement(44736..45323)
                     /locus_tag="VBE34_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEO7262981.1"
                     /translation="MKQLQEYIAFDLEFNTVGEHSHIIQVSAVKYSNHQEIALFDTYV
                     HTKVPLQSFINGLTGITARDIIGAPKIEIVLTDFQSFVGDTPLIGYNGYKSDLPLLVE
                     NGLDLTSQYQVDLYDEAFVRRSTDLNGIVNLKLTTVADFLGIKGKAHNSLEDARMTAR
                     VYEKFLDLDENKIYLKQQKEVAVDSPFATLGNLFD"
     gene            complement(45367..45903)
                     /locus_tag="VBE34_000227"
     CDS             complement(45367..45903)
                     /locus_tag="VBE34_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEO7262982.1"
                     /translation="MTVEKTVSELAEILGVSRQAMNNRVKTLPEECVEKNSKGVTVVN
                     RDGLIKLEEIYKKTILEEEPIDEEASRRELLEILVDEKNTEITRLYEQLKAKDIQIAS
                     KDEQLHVKDIQIAEKDKQLDQQQQLTLTAMEDTQRLQLELNEAKAEVEEIQEAKEEKI
                     QELEAVKKSFFGRFFNKK"
     gene            46038..47210
                     /locus_tag="VBE34_000228"
     CDS             46038..47210
                     /locus_tag="VBE34_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7262983.1"
                     /translation="MKHYDTIIIGGGPAGMMAAISSNFYGNKTLLIEKNKRLGKKLAG
                     TGGGRCNVTNNGNLDELLAGIPGNGRFLYSVFSQFDNHDIINFFQDNGVTLKEEDHGR
                     MFPTTDKSRTIINALENKIKELGGQIMTDTEVVSVKKIGDSFYIKTKDTQFASDKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLEVTDMEAAESPLLTDFPHKKLQGISLDDVTLSF
                     EKHIITHDLLFTHFGLSGPAALRISSFVKGGETIYLDVLPNISVKELEIHFQNEREKS
                     LKNALKILLPERLAEFYAEDLPEKVKQVSVKDLEMLIQKLKKLPILVTGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVAGLHFAGEVLDINAHTGGFNITSALCTGWVAGSLHY"
     gene            complement(47254..48036)
                     /locus_tag="VBE34_000229"
     CDS             complement(47254..48036)
                     /locus_tag="VBE34_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HEO7262984.1"
                     /translation="MCYNNSMNERYIRFNPNIAKQKPSNKDKCPFCDKSQLGKILDVK
                     DDMIWVENKYPTLEETYQTLVIESNDHNGDISVYSESKMRQLLDYLLSKWQLMEESGH
                     YRSVVLYRNFGPLSGGSLRHPHSQIVGLKNVDAYETLNLDWFDGIEVCSETSQQAEIS
                     IANRPMLDFTEFTIKVSKLENAILIADSLPILCHYILNEFLGGRCKSYNLFFYKHDGG
                     LICKIVPRFIVSPYQIGYAIHQVNLPDELEKIKEALRAKYYL"
     gene            48195..48464
                     /gene="rpsN"
                     /locus_tag="VBE34_000230"
     CDS             48195..48464
                     /gene="rpsN"
                     /locus_tag="VBE34_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEO7262985.1"
                     /translation="MAKKSKIAKFQKQQKLVEQYAELRRELKEKGDYEALRKLPKDSN
                     PNRLKNRDLIDGRPHAYMRKFGMSRINFRNLAYKGQIPGIKKASW"
     gene            complement(48825..49850)
                     /locus_tag="VBE34_000231"
     CDS             complement(48825..49850)
                     /locus_tag="VBE34_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEO7262986.1"
                     /translation="MLHFENDYNKGAHPELLNALIETNDQGLSGYGTDSYCQQAADKI
                     REVCSCPQAEVEFLVGGTQTNQVVISSMLASYEGVIAAETGHVSSHEAGAIEFSGHKV
                     LTLPSHNGKLLASEVATYIETFYADGNYQHMVFPGMVYISHPTEYGTLYSKAELEELS
                     KICKHYQIPLFIDGARLGYGLAAKDTDVDFPTIAALSDVFYIGGTKMGALAGEAVVFT
                     KKNRPKQFTTIVKQHGALLAKGRLLGLAFDRFFTDNLYLKIGKHAIDLAEELKIILEE
                     KGYSFYLKSPTNQQFIIVENTKLADLAKNVAYSFWEKYDDHHTVIRLATSWSTSREDV
                     TALRNVL"
     gene            complement(49970..50980)
                     /gene="tsaD"
                     /locus_tag="VBE34_000232"
     CDS             complement(49970..50980)
                     /gene="tsaD"
                     /locus_tag="VBE34_000232"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEO7262987.1"
                     /translation="MKDRYILAVESSCDETSVAILKNDKELLANIIASQVESHKRFGG
                     VVPEVASRHHVEVVTTCFEDALQEAGIVASDLDAVAVTYGPGLVGALLVGMAAAKAFA
                     WANKLPLIPINHMAGHLMAARDVKELQYPLLALLVSGGHTELVYVSEPGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMIKEDHLEFSFSGLKSA
                     FINLHHNAEQKGEALVLEDLCASFQAAVLDILLAKTQKALLKYPVKTLVVAGGVAANQ
                     GLRERLATDISPDIDVVIPPLRLCGDNAGMIALAAAIEFEKENFASLKLNAKPSLAFE
                     SL"
     gene            complement(51056..51463)
                     /gene="rimI"
                     /locus_tag="VBE34_000233"
     CDS             complement(51056..51463)
                     /gene="rimI"
                     /locus_tag="VBE34_000233"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEO7262988.1"
                     /translation="MESSEQALAIWSVLSDVYDKSPWSLSQISEDLKKDSTDYFFVYN
                     DGEVIGFLALQQLVGEVEITNIAVKKNYQGKGYAYQLMSMIADIEVPVFLEVRYSNIV
                     AQKLYERCGFVVLRKRKNYYHDPIEDAIVMRKG"
     gene            complement(51465..52157)
                     /gene="tsaB"
                     /locus_tag="VBE34_000234"
     CDS             complement(51465..52157)
                     /gene="tsaB"
                     /locus_tag="VBE34_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEO7262989.1"
                     /translation="MMKVLAFDTSSKALSVAVLNNMECLATVTINIKKNHSINLMPAI
                     DFLMQSIDLEPQDLDRIVVSEGPGSYTGLRVAVATAKMLAYTLKIDLVGVSSLYALTN
                     GFSENDLLVPLIDARRNNVYVGFYQNGDTVKPDCHTSLEEVLQEVGNKANVHFVGEVA
                     AFFDQIKKALPHAKITETLPCAVAIGRKGQKMKSVNVDAFVPRYLKRVEAEENWLKNH
                     CETNTEEYIKRV"
     gene            52336..52566
                     /locus_tag="VBE34_000235"
     CDS             52336..52566
                     /locus_tag="VBE34_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEO7262990.1"
                     /translation="MIYKVFYQETKERNPRREQTKTLYVTIDAANELEGRIAARKLVE
                     ENTAYNIEFIELLSDKHLEYEKETGVFELTEF"
     gene            52620..54299
                     /locus_tag="VBE34_000236"
     CDS             52620..54299
                     /locus_tag="VBE34_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO7262991.1"
                     /translation="MSNINLKPEEVGVYAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVENIDRIKALVITHGHEDHIGGIPFLLKQANLPIYAGPLA
                     LALIKGKLEEHGLLRDATLYEIHANTELTFKNLSVTFFRTTHSIPEPLGIVIHTPQGK
                     VICTGDFKFDFTPVGEPADLHRMAALGEDGVLCLLSDSTNAEVPTFTNSEKIVGQSIM
                     KIIEGIEGRIIFASFASNIFRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKINNIHTSGHGGQQEQKLMLRLIKPKYFMPVH
                     GEYRMQKVHAGLAVDTGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRHDLSEDGVVLAVATVDFDSKMILAGPDILSRGFIYMRESGDLIRESQH
                     ILFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDK"
     gene            complement(54457..54966)
                     /locus_tag="VBE34_000237"
     CDS             complement(54457..54966)
                     /locus_tag="VBE34_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7262992.1"
                     /translation="MEENMNIKQLKSKTMLGTVALVSAFSFASTNADANTYNYAVDVD
                     YLASAEEIAQAHPASNTFPLGQCTWGVKEMATWAGNWWGNGGDWAASAASAGYTVGTQ
                     PRVGSIVCWTDGSYGHVAYVTAVDPVTNKIQVLESNYAGHQWIDNYRGWFDPQNTVTP
                     GVVSYIYPN"
     gene            complement(55114..56460)
                     /gene="glnA"
                     /locus_tag="VBE34_000238"
     CDS             complement(55114..56460)
                     /gene="glnA"
                     /locus_tag="VBE34_000238"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEO7262993.1"
                     /translation="MTITAEDIRREVKEKNVTFLRLMFTDILGVMKNVEIPATDEQLD
                     KVLSNKAMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     EPFAGDPRGNLKRNMKRMQEMGYKSFNLGPEPEFFLFKMDENGNPTLDVNDKGGYFDL
                     APTDLADNTRREIVNVLTQMGFEVEASHHEVAVGQHEIDFKYDDVLKACDNIQLFKLV
                     VKTIARKHGLYATFMAKPKFGINGSGMHCNMSLFDNEGNNAFFDPEDPRGMQLSEDAY
                     YFLGGLMKHAYNYTAIINPTVNSYKRLVPGYEAPVYIAWAGRNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALSVLLGSGLEGIENKIEAPEPIETNIYAMTVEERRQAGIVDL
                     PSTLHNALKALEEDEVVKAALGTHIYTNFLDAKRIEWASYATYVSQWEIDNYLDLY"
     gene            complement(56494..56865)
                     /locus_tag="VBE34_000239"
     CDS             complement(56494..56865)
                     /locus_tag="VBE34_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7262994.1"
                     /translation="MKERELRRTMAVFPIGAVMKLTDLTARQIRYYEDQGLITPERTE
                     GNRRMFSLNDMDRLLEIKDFISDGLHISDIKNEYMQRQHKSKEKQKSLSDAEVRRLLQ
                     DELRNQGRFSSPSQHIGNMHL"
     gene            complement(56945..57484)
                     /locus_tag="VBE34_000240"
     CDS             complement(56945..57484)
                     /locus_tag="VBE34_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEO7262995.1"
                     /translation="MKTLKKLLSNYKFDIKKFKFGMRTFKTGLSVFLVLLVFHLFGWN
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMINVACNNKSGIIGGTAALLIITLSIPSGETILYVFARIFETFCGVFIA
                     MMVNTDIEILRKKLKNNKL"
BASE COUNT        19043 a        11182 c         9217 g        18158 t
ORIGIN      
        1 taataaagcg ttctccattt aatgacttct acgcaatgtt agcttacaaa aaggaactga
       61 gtaatattac tcagctccca ataaactatc ttgctaactt ctcttttaaa taatatcctg
      121 tataggattt aggattttca gcaacttctt ctggtgttcc gatagctaca atttgtccgc
      181 caccaatacc tccttcaggg ccaagatcaa taatgtggtc agcagtttta ataacatcaa
      241 ggttatgctc aataacaagc actgtattac catcatctac aaaacgatcc aaaaccttaa
      301 gtaaacgagc aatatcatct gcatgaagcc ccgttgttgg ttcgtctaat atgtaaagac
      361 tcttacctgt cgaacgctta tgtaattcac ttgccagctt cattcgctga gcttctcctc
      421 ctgataaggt tgtagctggt tgccccaaag tgacatatcc caaaccaaca tccttaatag
      481 tctgtaactt acgagcaatt ttaggaatag ctgcaaaaaa tgtaactgca tcattcactg
      541 tcatatcaag aatttgtgca atattttttt ctttgtaatg tacttccaga gtctcactat
      601 tatagcgcgt gccgtggcat acttcacaag gaacgtagac atctggtaaa aaatgcatct
      661 caattttgat gataccatcg ccagaacaag actcacaacg tccacctttg acgttaaatg
      721 agaagcgtcc ttttttataa cctcgaatct ttgcctcatt agtttgagca aaaagatcac
      781 gaatatcatc aaaaacacct gtataggttg cgggattaga acgtggagtc cgaccaattg
      841 gactttgatc aatatcaatc aatcgatcaa catattcaat gccttctaaa gaaacatatt
      901 tacctggttt atctgagtta cggttaagct tctgcgcaac agcttttttc aaaatactat
      961 taattaaggt tgattttcca gaacctgaga caccagttac ggcaataaat ttaccgagag
     1021 ggaacttgac atctaaattt tgtaggttgt tttctgccgc acctttgatt tctaaaaagc
     1081 gaccattacc tactcttcgt tcagatggca caggaatcac tttcttacct gacaaatatt
     1141 gtcctgtaat agatttagta tttttagcta cttgcttcgg tgtaccagag gccacaatct
     1201 caccaccaaa cgcaccagcg ccaggtccca catcgattaa ccaatcagct gccatcattg
     1261 tatcttcatc atgttcaaca acaataagcg tattacccaa atcacgcatt ttcttaagac
     1321 tatcaatcaa acggtcattg tctcgctgat gaagtccaat ggacggctca tcaagtacat
     1381 aaaggacacc tgataaatta gacccaattt gagtagccaa acgaatacgt tgactctcgc
     1441 ccccggaaag tgttcctgca gaacgtgaca gatttaaata attaagtcca acattattta
     1501 agaaacttag acggtcatgg atttctttga taattggtcg agcaattaat tgttcatttg
     1561 aagataaacg caatgtttct aaaagttcta aatggtctgc tatcgataaa tctgagactt
     1621 gcccaatatt aagaccttct tctcccccaa ctcttacgca aagtgcttga tcattgagac
     1681 gatagccatg acaagtgtta cattttagct cgttcatata ttctcgcata acattgcgtg
     1741 tgtaatcgct attagtttca tggtaacgac gattaatatt attgacaacc ccttcaaaag
     1801 gcaaatcaat atttctctcg cctccaaaat cgttaatata atggaaatgg aactctcgtt
     1861 cgcctgaacc atatagggct aggtcctgct ccgccttgct taatttttca aatggcgtat
     1921 ccacatcaac accaaattga gtcatagctt gctctagcat cgttggataa taatttgatg
     1981 aaattggatt ccaaggaact aaggcccctt ctctaagggt cttacttcta tctggaataa
     2041 ccaaatctat atctacttct aacttaattc ctaagccatc acaggtcgga caagaaccaa
     2101 agggagcatt aaaagaaaaa aggcgtggct ctaattcagg aacagtaaaa ccacattcag
     2161 gacaagaata atgctctgaa aacaataact cattgccatc cattgtatca ataacgacat
     2221 aaccatccga caaacgtaga gccgcttcaa cggaatcaaa taagcgggaa cgaatgccct
     2281 ctttattaat taatcgatct actacaatat caatattgtg catcttactt tttgataatt
     2341 caggcacctc agtaacatcg tgaatatcac catcaatacg aacacgaacg taaccatctt
     2401 tttgtatttt ctcaaatgtt gatttgtgtt gtcctttttt tcgacgtata attggggcta
     2461 aaatttgcat ctttgttcgc tctggaagcg ctagtacctt atccacaatt tgctctacag
     2521 aagatgctgt gatggctccg tgaccattga tacaatatgg ggttccaaca cgagcataaa
     2581 gcaaacgtaa ataatcgtta atttctgtta cagtcccaac agtagagcga ggattcttac
     2641 ttgttgtttt ctgatcaata gaaatagctg gactaaggcc atcaatagaa tctacatcag
     2701 gcttttccat atttcccaaa aattggcgcg cgtaagcaga aagactttca acgtaacgac
     2761 gttgtccttc tgcatagata gtgtcaaagg ccaaactaga cttacctgat cctgatagtc
     2821 ccgtaacgac aactaacttg tctcgtggaa tgtcgacact tatatttttt aaattgtgag
     2881 ctcttgctcc tcttatcatt aatttatctt gcataatgtt gtgcctcagc actccttttt
     2941 gaatagtgtt gacttattat atcaaatttt aataaagtca gtattttttt agttttaaat
     3001 ttcatagaaa aaagagatga actaatacgt tcatactctt tgttattatc ttctttgttg
     3061 tggtgccact ggtgccattg agctttgaat attatattta cgttgtaaat aaaagcgcaa
     3121 accaaacgat gctaaagcaa taataaacaa aacaacctca ggaagtttag gattaatagt
     3181 tgcaggtaga agaggtacta ctgcaaataa ggcaaaccaa actaacataa ctagtgccag
     3241 tactgcgaaa gatttaagcc agcctggtct ttcagatttt ggtttaccca tatggcgata
     3301 aatatagtaa taagtaacgt acatggccgc accaccacca aaaccaagag ttaaaattga
     3361 aataagccct gtattgacat tagattgacc aaatagcaac attaaaccat tcaaagcagt
     3421 tactaagcca agaaatagta aggaagtatc taaccacatc aaccaaggat tggtattttt
     3481 ttgtacagat actctagctt tatcctcagg tcgctcagtg aatgaggctg cccaagtagt
     3541 tggtgctcct aagagtgagc gtgcggtaat tccttttttc tgattatcta taatctctgg
     3601 gagatgttct tctaaaatag ctttgatttc tgcatctgat tttccatcag ctaaaagttg
     3661 attagttgca atatgaacaa actcttgatt ctttttagtt aagtcttgta aagtcatgtg
     3721 aactccttga ttaatatact attaaaacca ttttttacga ataaaataaa atacaacaaa
     3781 tgaactcatc aaaaaggcca aaagaacaac atagataaag ccatgtgcga gaccattcaa
     3841 aggcatccaa ttatttttaa agttcatccc atacgccgaa aaaataaccg ttggaatatc
     3901 caatgtcata gttactaggg ctaaggtttt cataatggta ttctgattat taccgatgat
     3961 tgaagcagtc gtttctgtca tggcatttaa gacattttca tagatatttg ccatttcaat
     4021 agcctgctga gtttcgatca atgtatcttc aagcagatct tcatcttcaa tgtatttttt
     4081 taaactactg gtactactgg tcaatttttt gacgattcgc tcattaaatt ttaaagaggc
     4141 ctttagatag acaatagatt tttccaattc catcatatcg atcaattgct catttcgagt
     4201 tgcactctct agttgtgctt caattttatc gctctgccta tcgatagtac gcaaagcctg
     4261 taagtataat tcggcattgc gataaagcaa ttggaaaaca aaacgtgtct tcataaaggt
     4321 ataaaaattt ttgacacgtc gtctataaaa gtgatcaaac agcgtcaaat gttctaggca
     4381 agtagtaata acagcgttgt cagtaacgat aatgcccata ggaatagtca tataataact
     4441 tttgttattg cgttcttcat aggtaggaac gtcaacaata actaaagtat aatcatcttc
     4501 aaccgagata cgggaagttt cttccacatc gaggggagca cgtaaatcat caatatcaat
     4561 accaaattga tcagctactt gttcggattc ttcctgggag ggattaacca gatttatcca
     4621 agcacctggc tcgaatgttt caatttcttt aaattcaatt gccgttgaaa gaaacatttg
     4681 cttcatggcg attcctcctt tgttataaaa aatccatacc ttagtattat acaggaaaac
     4741 catttgtttt gcacggttaa tacggtggta tcctaaattt ttctattatt tgactcgtaa
     4801 ataccaataa taatggtaaa aagaataaaa aaatagccgc tagggctatt ttatattaat
     4861 cttcatttac gtaaggcata agagccataa cacgagcacg tttgattgct gttgttactt
     4921 tacgttggtt tttagctgaa gttcctgtta cacgacgtgg taaaattttt ccacgttctg
     4981 aaacgaaacg gctaagaagc tctgtatctt tgtaatcaac atattcaatc ttgttagctg
     5041 cgatataatc aactttttta cggcgtttga atccgccacg acgttgttga gccatgttta
     5101 tttctccttt atagttttaa attctgtcca tctattatat tagaatggta gatcatcatc
     5161 tgaaatatcc attggatttg agttaccaaa cggactctca tcacgactaa aattaggtgt
     5221 ttgttgagaa gaagctgaat aactattatt tgacggagca ttgttatagc caccgttatt
     5281 ataagagtta ggtgaaccac cttctcgtgt agcacgactt tccaataatt ggaaactttc
     5341 cgcaacaact tctgttacat agacacgttg accttgttgg ttttcataat tacgcgtttg
     5401 gatacgacct gtaattccaa ccaaagcacc tttttttgcc cagttagcca agttttcagc
     5461 ttgttggcgc caaataacac agttaataaa atcagcctca cgttcgccag attgattttt
     5521 aaaattacga ttaactgcaa gtgaaaaagt ggctaccgct tgattacttg gtgtataacg
     5581 aagttctgca tcacgggtca tgcgacctac aagtactaca ttattaatca taaactacct
     5641 tcttagtcta ctttaacgat catgtgacga agaatgtcac cgttgatttt tgaaagacga
     5701 tcaaactcat taagagctac tgcatcttca gcttcaacgt taacgatatg gtaaagtcct
     5761 tcagtgaaat cttggatttc gtatgcaaga cgacgttttt cccaatcttt tgattcaaca
     5821 acagttgcac cgttgtcaga taagatagaa tcaaaacgtg ctaccaaagc gtttttagct
     5881 tcttcttcaa tgtttggacg aataatataa agaatttcgt atttagccat tgatatgttc
     5941 ctccttttgg tctaatgact catgaatttt tcatgagtaa gtgaggttat gctcacaaga
     6001 tattattata gtagaaataa gttaataatg caaggttttt atgtttttat ttccctgata
     6061 tcatttaaag acatttttta tttttcttta ttgaaatatg ccttatatca aacctatgta
     6121 gccattttct tttactgact tatgttcaat ccccattcta taattcaatt aaaaaataaa
     6181 aaggcgaggt ttacacctca ccttcaacat attatttaaa gaattcttta ttggtttaag
     6241 ttttcgttgg agaaaaattc agtgtctccg attttatatt tttaaatatt aaaatactat
     6301 ttagggagtc cctctttatg ttttattacc tttggtaaca ccaaaacata cacgaacttt
     6361 tattcatatg tgttaccatt ttacatcttt catattgaga tatttgatta gcaatacctc
     6421 cttgactaac aaaaattact aacacatcta ggggtatacc ctagtttata tttgttcatg
     6481 attacctcaa gcgagcatgc gctaatggta atgaactatt accacattgt ctgtcttcct
     6541 taaattatat aaggacttat gtgtcaccgt tactgtagcg acctacatct aaacctaaca
     6601 taagtgcccc tccaactatt tctaggaaat gaagaaaact tcaaatcctt gtgactgttt
     6661 gcaacatcaa tgcattgata acaattttag tgtatcgcca ttcttatagc gacccatatt
     6721 aaaaattaac ataagcgcta cctccattaa ctaaagttag cttgcctacc aatctttctt
     6781 ttggagacta gtctccattc ttatacctag agatattgta atagatcatg gataacctcc
     6841 ttgatttaaa ctttatcttt ataatttaat tatagtgtaa acgcttcctt aagtcaagaa
     6901 aaaaatattg atgcaacatt tttcttcatc attaagtacg atctagtcaa catacctctt
     6961 tgtcaaatcc tctttctttg atacaattaa tctatgatag atttaaaaga ctatggaatt
     7021 gagatgtggc ctgaagatag aatcgcttct tttcgtcgca cacttttagg gtggtatgac
     7081 caagaaaaac gtgatctccc ttggcgaaga actactaatc catactatat ttgggtttct
     7141 gaaatcatgt tgcaacaaac acaagttaat acggtaatcc cttattataa acgttttcta
     7201 gagtggtttc ctcaaataaa agatttggca gacgctcctg aagaacaatt actaaaggca
     7261 tgggagggac taggctatta ttctcgggtg cgtaatatgc aaaaggctgc acagcaagtg
     7321 atggttgatt ttggtggcat atttcctcac acttatgatg atattgcttc attaaaaggg
     7381 attggtcctt atactgcagg tgctatcgcc agcatctcat tcaacttacc agagcctgct
     7441 gttgatggca atgtgatgcg tgtcatggct cgtttgtttg aagttaatta tgacatcggt
     7501 gaccctaaaa atcgtaaaat ttttcaagct attatggaaa tactaattga ccctgacaga
     7561 cccggtgatt ttaaccaagc acttatggat ctgggtacag acatcgaatc cgctaagaca
     7621 cctagacctg atgaaagccc tattcgtttc tttaatgctg cttatttgaa tggtacttat
     7681 agtaaatatc ccataaaaaa tcctaagaaa aagcctaaac caatgcgaat tcaggctttt
     7741 gtcattcgta accaaaatgg tcaatatcta ctggagaaaa atactaaagg gagacttcta
     7801 gggggttttt ggtcatttcc tattatcgaa acgtcacctc tttctcaaca attagattta
     7861 tttgatgaca atcaatccaa ccccataatc tggcaaaccc aaaatgaaac tttccaaaga
     7921 gaatatcaat taaagcccca atggactgat aatcactttc caaatattaa gcacactttt
     7981 agtcaccaga agtggacaat agaactcata gagggggtgg tcaaagcaac agatttacct
     8041 aatgctcctc acctaaagtg ggtagcaatt gaggactttt ctctgtatcc ctttgcaaca
     8101 ccacaaaaga aaatgctaga gacctactta aaacagaaaa atgcttaatc acaagtgaac
     8161 tatgacggtt cactttttgt ttacaattcc tttacgccgc tgtaaagttt tcctcagatt
     8221 atcttttaat accctataaa tagtggtcta tccctaatgt caaatacatt tgatagaccc
     8281 gttagcttat catccttata ctaatattat gaaaggagat atttatggaa attggtcagc
     8341 aaattattcg ctaccgtaaa caacaagcgc tctcgcaaga agaattagcc gagaaagtct
     8401 atgtaagccg tcagagcata tctaactggg aaaatgacaa aacttaccca gacatccata
     8461 gcctcttact cctaagtcaa atttttcaag tgagcttaga ccaactaatt aaaggagaca
     8521 ttgagaaaat gaaatatacc atcactcagg ttgataaaaa gaattttgaa cgagatacca
     8581 aagtaatggt gacattgatg atactattaa tgatttcaag ttatccatta gtctatttct
     8641 tagagtggct aggacttggc atttttgttt tattatcgat aattactatg acttatgcta
     8701 atcgtgtgga acgttttaag aaaaaatatg atgtgcaaac ttataaggaa atcctagctg
     8761 tttcaagtgg aaaattatta gacgaaatcg aaaaacggga agaaagagca aaacttcctt
     8821 atcagaaacc ccttattgtc actgtatttt tcctcattac tgttgccacc ttttttgcaa
     8881 gtcgttttat tttcacatgg ttgttccatt taagttagta gataataaaa taaagctcag
     8941 cgtgacactg ggctttattt tatttaactc aattcagcaa taatagcttt cagttggtct
     9001 ttagtatgaa cacctgcaac ttgtttaacc acttctccgt cttttttaaa catcaatgtt
     9061 ggaattgaca taataccaaa ctgacgggca gtttccggat tctcatcaac atccattttc
     9121 aaaattttga gttcatcttc atcgatttct tgagataact gttctaagat tggtgcttgc
     9181 atacgacatg gaccacacca agttgcccaa aaatcaatga gtactaaacc ttctttagtt
     9241 tcttctacaa atgtagcatc tgttacttct aatgccatct tttttctcca tttcatctta
     9301 atgataagcc tattgtacaa gaatttagtt gtttttgaaa ctatttgcta cgaactcgct
     9361 aaataaataa acaaagccct aaaatcaagc caataaggta acctacaata acatccttag
     9421 gatagtgaat acctgctatc acacggcaaa ttgccaatag aaccgataat attaaacaaa
     9481 ctattccaaa gtaaacataa tatcgtaaca aacacatact aatcatcgta gctgaaaaaa
     9541 catgtctgct aggcattgac cttccctcag tatccttata aattaaaggt ttaatattcc
     9601 atttttcata tggtcgtgga aaatcaaggc gtttcctaat gtaagacaaa gctataaagc
     9661 caacagcagg gataattaat agccttaacc ataattgaaa tgtcatccct tgccaaagag
     9721 taaatatcag aaaagatgga tagagtatgt acatcattcc tgtgactatt tttagtaaaa
     9781 aatttaaaag aattattaac tgtggggatt ttctaaaagg ttgagatagt ttggcataaa
     9841 attgttcata tgattccata atttacccta acgttacaat tgtggcgcca gatccccctg
     9901 cattttgtgg ggcataagca aaatgcttaa cgtgcttatt acgacgaaga tattttgtca
     9961 ctccctcacg gataacgcct gtaccaatac catgaatgat atcaacttgt cccatattgt
    10021 taagcaatgc ttgatcaata aaattatcta actcttgcat agcttcttcg tatcttttac
    10081 ctctaagatc aagtcgagct cttggtccag aactatcagc ctttttaacc acattaatct
    10141 gtttactttt gactttctgt tcttcttgga ctctaacaag ggtaaattca tcttgtgtta
    10201 atgtcatttt gataattccc acttgtgctt cccaacgtcc atcttttaat tgactagtta
    10261 aggtacctcg ctgtccatag ctagtcacta taatatcatc accaattcta ggagcacgag
    10321 ctgctttaat ttttttagcc ttatttaaga ctttattttt tgataaatca acttgaggtg
    10381 ctaatttttt tatttgtgcc ttagcatcta taatttcgtg aggttttaat tggctcttat
    10441 cattgagttt ttttaagata gtatcactct cattcaaagc catatctaca atttcttgag
    10501 cttcttgata gattttttct aactctttat cgcgctcatg tgaaaattca ttatagagtt
    10561 tcttaaccgc acgattgaat ttgaggtttt cttgttcaac ttctttaata tgatccagtc
    10621 ttctacgtgt ctcaagtgtc tgtgcctcta actgttcaat aatacggtta acatctgagt
    10681 cagaatctgt catctgctta gcttgtttaa caataaatgg agctaaacca aggcgagaag
    10741 caatttcaaa tgcatttgat cgtccaggaa ctccttgcat aaagcgatac gtagggctaa
    10801 gcgtttcggc atcaaattcc atgctcgcat tctctacaaa atttgtctca atcccataag
    10861 cttttaattc tggatagtgc gtggtcgcca tcgttttgat attacttaac ctaaggtgtt
    10921 ctaaaatagc catagccaaa ctagcacctt cttgaggatc cgttcctgct cccagttcat
    10981 caaagaggac taaactattg tggtcagcct cgtttaaaat actgactatg tgcgtcatat
    11041 gactagaaaa agttgataga ctttgttcaa tagattgctc atcgccaata tctgcaaaga
    11101 tattgttaaa tactgcaatt ttactacctt tatccgctaa tactggcaaa ccagactgtc
    11161 ccattaattg tgctaaaccg agtgttttta gcataatcgt cttaccacca gtattgggac
    11221 cagtgatgac aattgcagtt aaatcttgat caaaatgtaa gtcattagcc acagggttac
    11281 ttaacagagg atgacgaaca ttgattaatg ctaacgtgct gtcattagaa atctcaggta
    11341 tcgtcgcctt attatcagac ataaaaagat atttagccct tacaaaatca agatgcccaa
    11401 gaatccatgc attatttcta atagtggcga catggggtct taacaagtct gaaaatgcgt
    11461 gtaaaatacg actttcttca tgacgttcgt cagctctaag ctgcgttatc tcttcgttta
    11521 gtgtaactac agcacgaggc tcaatataaa cagtacttcc tgaagaagag atgtcatgaa
    11581 ccacaccaga aatacgattc cgataagtat tttttactgg taggacactt cgtccactac
    11641 gactagcgat tagattctct gataaaagct ctgctttttc cttaagcata tcctgtaaaa
    11701 tctgacgaac ccgtcgttca ctgtttgtta attgacgacg gatacgctct aattctggac
    11761 tcgcaaaatg ttctaaaaaa ccaccatcat tgatagcttg aaaagaccct tgcagattag
    11821 ggaattgttc caaattttca aacaaacgat ccaaagattg gaaagaaaca ttatccaaat
    11881 cagaataaaa gtgaatcata tccgaagaac tctgtaaaac ttttttgata gccaaaagtt
    11941 cttgaatatt aagatcagct gaaagctcta agcgttttaa actctcagag attgaactta
    12001 ggctgactat gccaaatgag tgatgttcaa caaaaatctg ttccatgtca gaaatttcat
    12061 taaaactttt ttctatttta ggagcctctg tcatcggctc cagctctgat aattcttctt
    12121 gtgattgttc tgtcttgaga taaggtaata tcaattcctt aactttgtta aattctaact
    12181 gttctaaaat cttgttattc atactcttca ttatacctaa atttatcaca aaatgacaac
    12241 gttaaaaagg ttggaactct tactccaacc ttactaaaca ttatattcaa agacctaccg
    12301 cccaagtcct taccaaaaac tgtgagaaga atggtaagtc gttaattaaa aatctaacaa
    12361 acagactatt ccataacttt tcttgaacaa atgacatagg gactgttgcc agaatagtca
    12421 aacacatact cataaataag agacaaacca gaacggctaa aaaaccacta ataatattat
    12481 tttgcaagct atcaaaatgt tcaactggtg ctaaatgtag taaaacacct aacaagcgac
    12541 ctactaagta agataaactg tagataaaaa caaaggcaac acctgcataa taaacatgac
    12601 tcaattgaaa tttgctaata tctttaaaaa agtaaacttc tactccttgt acaggattag
    12661 aatacggtac ccataacgta atctgactag ctaattcttg gtaaaattga ctagcaatca
    12721 tcaaagatac cattgacatt aacacgtaaa agacttgtaa aaaaatacct ctactgtagc
    12781 caatataaaa atgccaaata acaataatca gcaaaagcaa tgatagcatg attaatcctc
    12841 actcactttt tctttaatgt ccgaaatcgt tttggagcgt aacttattga gctcatcttc
    12901 cattttttca atgtcaattt ctcgacttag ttgcactgat aaagcattaa ttgccatgag
    12961 aatagcaatc gtttcattat cagcattagg taatttttct ttaatagctt catacttttc
    13021 tgtagctaca cgctcaatct cttccataaa aaggttgtct ttatctgtcg tcaatgtgag
    13081 aggcttatct ccaaagacaa atttataacg atttttctga ctattattta tttttgtttc
    13141 catactatac ctctgctagc attataccgt aaaatagggc tttcgtaaaa ttctacccct
    13201 tatcattact tgtctattcc cttattcctc ttttttatga tagaatattg tctatgaaca
    13261 caatcgtcat gcaagcagat aaaaaattgc aagaaaagat aagaaccgat ttagctcaac
    13321 atcatataag taataacaat ccttatgtgg tattttcagc taaaatatca ggagctactg
    13381 ttcttttata tacttctgga aaacttgtct tccagggttc taacgcttca catatcgccc
    13441 aaaagtatgg ttttattgag caaaaagaaa gttgttcctc tgagagtcaa gatattccca
    13501 taattggtac tgatgaagtg ggaaatggct cttactttgg tggactggca gtggtagcta
    13561 gttttgtaac tcctaaggac catgcttacc tcaaaaaatt aggtgttggt gattcaaaaa
    13621 cacttactga tcaaaaaatt aagcaaatcg cgcctctttt ggaaaaagca attccacaca
    13681 aagcattatt gttatcgcct caaaaatata atcaagtggt tagtccaaat aacaaacata
    13741 atgctgtctc cgtaaaagtt gctttacaca atcaagctat cttccttctt cttcaagatg
    13801 gttttgaacc agaaaaaatc gttattgatg cttttactag ctccaaaaac tatcaaaatt
    13861 atttgaaaaa tgaaaagaat caatttaaac aaacgattac tctggaagaa aaagctgaaa
    13921 ataaatatct agctgtagcg gtcagttcta ttattgcacg taatctattc ttagaaaacc
    13981 ttaacaaatt aagcgacgac gtaggatata aacttccaag tggagctggt caccaatctg
    14041 ataaggtcgc tagccaatta ctcaaagctt atggtatctc ttcgcttgaa cactgcgcaa
    14101 aacttcattt tgccaacacc aaaaaagcac aagcattatt aaaataaata gcagggaaaa
    14161 ttatgaaaga atttattaaa gaatggggtg tctttatcct catcctctca ctttttttac
    14221 tatcgcgtat ctttttatgg caattcgtta aagttgacgg acactccatg gatccaactt
    14281 tagctgacaa ggaacagcta gtagttctca aacaaacaaa aatcaatcga ttcgatattg
    14341 tagtggctaa cgaagaagaa ggcggccaaa agaaaaaaat tgttaaacgt gtcattggta
    14401 tgccaggtga tgtcatcaaa tataaaaatg acaccttaac tattaacaat aaaaaaacag
    14461 aagaacctta cctcaaggaa tatactaaat tatttaaaaa ggataaatta caggaaaaat
    14521 attcgtataa cccacttttc caagacctag cacaaagctc taccgctttc accactgaca
    14581 gcaatggcag cagcgaattt actactgtcg tgcctaaagg ccactactat cttgttggtg
    14641 atgaccgaat tgtctctaaa gatagtcgtg ccgtcggttc cttcaaaaaa tcaacgattg
    14701 tgggagaggt taaattccgc ttctggccaa ttcgtcgttt tggaactatc aactaaagga
    14761 aattaaaagt tcacctgaca aaagttgaaa tagcatatta tattgaaaat agggagtgac
    14821 aaatccattt aaaataggga tttacaatca ctccctttaa ctgtttatta aaaaggaagg
    14881 tacaatggaa gtatttttta caggaaccat tgaacgcatt atatttgaaa atgccagcaa
    14941 tttttttaaa atattacttc tggaaatcga agatacggat agcgattttg atgatgtaga
    15001 agtaattatt actggtacta tggccgacgt tatagaggga gaagaatata ctttttgggg
    15061 aacactaacc caacatccaa aatacggcga gcaactacaa agtgtacgct atgaaagagc
    15121 caagcctact tcaggtggcc ttgtgaaata cttttctagc gaacaattta aaggaattgg
    15181 taaaaagact gcccaacgca tcgtagaact ctatggagat aataccattg ataaaatcct
    15241 tgaatcccct gaacagttaa gtacaatctc aggactatca aaaattaata gagaagcatt
    15301 catcgctaaa ctgaaactca attatggaac agagcaagtt ttagctaaac tcgctgaata
    15361 tgggttaagc aatcgtgctg ctattcaaat atttgatcac tataaagaag aatcacttga
    15421 agtgataaac gaaaatcctt atcaacttgt tgaggatatc caaggtatcg gttttaaaat
    15481 agctgaccaa cttgctgaac aagtcggtat tgaaagtgat tctcctaaac gctttcgtgc
    15541 tgctattatt catactttag ttgagtcttc tatggagcaa ggagatacct atattgaggc
    15601 tcgtactctc ttggaaaaaa ccattactct ccttgaagaa gctcgtcaaa tcgaactaga
    15661 tccttcaatt gttgctaagg aattgacaaa tttaattgca gaagataaag ttcaacatat
    15721 tggaactaaa atttttagta atactctatt ttttgccgaa gaaggcatca aaaaaaactt
    15781 acaacgaata ctaaatcagc ctcttgataa acaactaaat cacaaagata ttgaccgtga
    15841 aatcagagat attcaaaaat ctttaaatat ccactacgat aatatccaag aaaaagctat
    15901 tagagaagct ttactaagta aggtatttat cttgactggt ggcccaggaa caggtaaaac
    15961 aacagttata aatggtatta ttgaagccta ttccgagtta catcatattg atttaaacaa
    16021 aaatgatatt cctattgtac ttgctgctcc tacaggtcgt gctgctagac gcatgaatga
    16081 acttaccggt ttaccgagtg ctactattca taggcatttg ggattaaacg gagatagtga
    16141 ctatcaaagt ctagatgact atctagattg ttccttaatt atcattgatg aattttcaat
    16201 ggttgacact tggcttgcta atcaattatt tgacgctttg gactcgcaca cacaagttat
    16261 tattgtcgga gatagtgatc aattaccgtc tgtaggtcca ggacaagttc tagcagatct
    16321 tttaaatata aatgcacttc cacacgttaa acttgaaaag atttttagac aatcagaaga
    16381 atcaactatt gtgactttag ctaatcaaat gagacagggc tttttgccag aggactttac
    16441 agctaaaaaa gccgatcgct catactttga agcatctgct aatatcatcc caaatatgat
    16501 ttctaaaatt gttcaatctg cacttaagag cggtattgaa gctcatgaaa ttcaaatttt
    16561 agctcctatg tatcgcggcc aagcaggtat taataatctt aaccttatca tgcaaaatct
    16621 cctcaatcca ttaaaagata ataaccaatt tacttttaat gatataaatt tccgaatagg
    16681 agataaagtg ttacatttag tcaacgatac agagcttaat gtttttaatg gggatattgg
    16741 ttacataacg gaccttatac cagccaaata tactgagagc aagcaagatg agatttatat
    16801 gacttttgac ggtcaagaag ttatctatca acgcaaagaa tggcttaaaa taacactagc
    16861 ctatgctatg agtattcata aatcacaggg ttctgaattt caagttgtta ttctgccaat
    16921 aacgagacag agtggtcgaa tgttacagcg aaaccttatt tatacagcta ttacgagatc
    16981 taaaagtaaa ctaatacttt taggagaaat tggtgcattt gatttcgctg ttaaaaacga
    17041 gggggcaaag cgcaatactt atctgataga aagatttgaa aacaagcaag aaatagctaa
    17101 ttctcaaaaa attgaagata gctcaataga tcaaaagatt gacaacacaa taattaatac
    17161 gtccattcca aaaacagcaa caccaattga gcagactaat ctttcaaaaa taacttatcg
    17221 cctaactgaa gaaaattatt taacaatcga tccaatgatt ggtattaatc aacaagatat
    17281 aagtgcaatt tttgatagta aataaatttt ttcataaaat cttgaaaact cttccaaata
    17341 tgttataata tcattgtatt aagaacgtag ttcaaattta atacttttat taaggtttat
    17401 ttagaaaaag gagatattat ggtttcatat gaaaaagtcc gccgctctct gcgcacagct
    17461 acaatcacta tcattgtatt aaatagtttg tctttagtat tcagattgtt tacaggaatt
    17521 tcagtgcagc tagccaaaac tgaaattaat aaagggaaca caggtaatct tcctaaagaa
    17581 catatcgaag cagtcttgag cgccacaaca ccatttatgc tatttgtcac tgctttaata
    17641 gtattagtca acattgccat tgttattttc tgtattaaaa atttaagagc aattaaaaga
    17701 aatcaaatgg ttaattacct tccttattac cttggcttcg ccattactgt aggacttgtt
    17761 attttgggtt ttctaactac taaagcacca tgggcaatag ctataaatat cgtcttccaa
    17821 gctattttcg gtcttcttta tttccatgct tatcaaaaag cacaaaaact caacgaacgt
    17881 gatcttgaag agactaacta aaagtaaaag cactagaatc tagtgctttt ttcttttcaa
    17941 agagagttaa atatagtcat cgtcccaact tttaaaaatc taaatagata gcttcatgtt
    18001 cattttccag ggtcgttact gtaacaccta agagtcgtat gccaaaaact gactcttcta
    18061 aggtatcaaa tatcgcttta gcaacttgat caatgatttg aaaatcttga gtatactctt
    18121 ctaatgtcat acgtttcgtc aatgtttcaa aatccgcata tctaactttt aaaacgattg
    18181 tcttaccaac tttcttattt ttctccagag aagcaacaac tctctgaacg ttctttgaaa
    18241 tctcagcttt gatatctgct tcgttatata gtaatttccc ataggttttc tcacttccta
    18301 ttgatttacg aactctatta ggttttaccg gggaagcatt aatccctctt gccttacgat
    18361 aaaggtcata cccgaaacga ccaaacatat caattaaaga aatttcagat aaggaaagga
    18421 ggtcctcacc agtataaacc cctaaagcat gtaatttttc gacggagcgt ttaccaacac
    18481 catgaaattt ctctattggt aagggcttca agaaatcttg agcttgatct ggtaggatga
    18541 aggtaagtcc ttttggtttc tcaaagtcag aagccaattt tgccaaaaat ttattgtagg
    18601 aaatacccgc tgaacaggtc aaatggacat cattccaaat atcgtattgt atcattttag
    18661 caagtttaac agctgatttt attcccattt tattttcagt tacatctaaa taagcttcgt
    18721 ctatactcat cggttcaacc aaatccgtat actttttaaa tatatctcgt acttccattc
    18781 ctacttggcg atatttttgg taatttcccg aaataaagat agcttgaggg catcgttcat
    18841 acgcttcttt tgaagacata gctgaatgaa ctccaaactt tcgagcctca taattacaag
    18901 tagaaacaac ccctctgccg ccggtttttc tgggatcaga accaataata acaggctttc
    18961 ctttaagaga gggattgtca cgctcttcca cagatgcaaa aaaagcatcc atatcaatgt
    19021 ggataatttt acgcgacgta tcatttatta aaggaaaaat taacattgtt ctctccccca
    19081 ttctctctac tccattataa cagattacaa cttaatgata cagattaaaa aaactgtatt
    19141 ataaaagaat cataattttg tcaagaaata tgcttgcgaa aacgtttact ttttcctaaa
    19201 atggatatgt aaacaaaatt aaatctgtca caatgtttaa ggagaaaact atggcaacag
    19261 ttaaaacaaa cacagatatc tttgaacaag cttgggaagg ctttaaaggt gttgattgga
    19321 aagaaaaagc aagtattgct cgcttcgttc aagcaaacta tgcaccttac gatggagatg
    19381 aaagcttctt agctggagca actgaacgtt cacttcacat caaaaaagtg attgaagaaa
    19441 caaaagctca ctatgaagaa actcgcttcc caatggatac tcgtgttgct tcaatttctg
    19501 aattaccagc tggttttatc gacaaagata acgagcttat ctttggtatt caaaacgacg
    19561 agcttttcaa attgaatttc atgcctaaag gtggtatccg tatggcggaa actacactta
    19621 aagaaaatgg ttatgaacct gatccagctg ttcacgaaat cttcactaag tacgctacaa
    19681 cagtaaatga tggtatcttc cgcgcttata cttctaacat tcgtcgtgct cgccatgcac
    19741 acactgttac tggtcttcca gacgcttatt cacgcggacg tatcattggt gtatatgctc
    19801 gtctagctgt ttacggtgct gactacttga tgcaagaaaa agttaatgac tggaatgcat
    19861 tgaatgatat tgatgaagag tcaattcgtc ttcgtgaaga aatcaacctt caataccaag
    19921 ctcttggtga agttgtaaaa cttggtgacc tttacggcgt agatgttcgt aaaccagcca
    19981 tgaacactaa agaagccatt caatgggtga acattgcttt catggctgta tgtcgtgtta
    20041 tcaatggtgc tgcaacatca cttggacgtg ttcctattgt ccttgatatc tttgcagaac
    20101 gtgacctcgc tcgcggtaca ttcacagaat cagaaatcca agaatttgtt gatgattttg
    20161 tccttaaact ccgtactgta aaatttgctc gtactaaagc ttatgatgcc ctttactcag
    20221 gtgacccaac ctttatcaca acttcaatgg caggtatggg agctgatgga cgtcatcgtg
    20281 ttaccaaaat ggactatcgt ttcttaaata cacttgacaa tatcggtaac tcaccagaac
    20341 caaacttaac tgttctttgg tcagatcaat taccatatgc tttccgtcgc tactgtatgt
    20401 caatgagtca taaacactct tcaatccaat atgaaggtgt atcaacaatg gctaaagaag
    20461 gctacggtga aatgtcatgt atttcatgtt gtgtatctcc acttgatcca gaaaacgaag
    20521 acaaacgtca caaccttcaa tattttggtg ctcgtgttaa cgttatgaaa gcacttctta
    20581 ctggtcttaa cggtggttat gatgatgttc ataaagatta caaagtattt gacatcgacc
    20641 caattcgtga cgaagtactc aactttgaca ctgtaaaagc taactttgaa aaatctcttg
    20701 actggttgac tgatacttat gttgatgcga tgaatatcat ccactacatg actgataaat
    20761 acaactatga agctgtacaa atggcattct taccttcaca tgtacgtgct aatatgggat
    20821 ttggtatctg tggttttgca aatactgtcg attcattatc tgcgattaaa tacgctactg
    20881 ttaaaccaat ccgtgacgaa gatggttaca tctacgacta cgaaacagtt ggtgacttcc
    20941 cacgttatgg tgaagatgat gatcgtgttg actcaatcgc tgaatggtta cttgaagcct
    21001 tccatggacg ccttgctaaa cacaaacttt ataaagatgc tgaagcaact gtttctcttc
    21061 ttacaatcac atcaaacgtt gcttactcta aacaaactgg taactcaccg gtacataaag
    21121 gagtatatct caatgaagat ggtagtgtaa acctttctaa agtagaattc ttctcaccag
    21181 gtgctaaccc ttctaacaaa gctaaaggtg gctggttaca aaacttgaat tcactttcta
    21241 aacttgattt cgctcatgca aatgatggta tttcattgac aactcaagtt tctccacgcg
    21301 ctcttggtaa aacatttgat gaacaagttg ataacttggt aacagtcctt gatggttact
    21361 ttgaaaacgg tggtcaacac gttaacttga atgtcatgga ccttaaagat gtctatgaca
    21421 agattatgaa tggtgaagat gttatcgttc gtatctcagg ttactgtgtc aatactaaat
    21481 atctaactcc tgagcaaaaa actgagttaa cacaacgtgt cttccatgaa gtactttcaa
    21541 tggacgatgc attaactaac tagtttgtat tcgatgaagt aggtttgatt atatcaaacc
    21601 tacttttatc tatattatca atagtactaa ttacttcttt atataacctc aaggaggggc
    21661 ctaatgttaa atcacaaatt tcttcaagtt ctaaaatatg agggtgtcgt ttcgataaca
    21721 agctggaccg aattagctcc tcacgttaca aacacttgga atagctacct gactattaca
    21781 gatgaccagc gtatattagc tcctgcagca ggaatgactc atcttgaaat gatctcaata
    21841 ataattctaa aattattatg actctgggca gccgtgaagt cgagggtcgt gatggttatc
    21901 aaggtacagg ctttagaatt gaaggaactg ctaagctatt agaagcagga agcgattttg
    21961 aaattgttaa agaaaaatac ccttttctgc gtaaagtatt ggaagtaact cccataaatg
    22021 ttatccaact tttataaaaa acttctccta gaaaattcta ggagaagttt tttattcaaa
    22081 gtacgatgtt atcgcctctt gtacatgtcg tcgtttctta atccaaagtg gacggtcatc
    22141 ataggaaaag gttctgtttg tcaaaaaaat agctgctgcc tgcttttgat aatttagtgc
    22201 aataaaagga ccagtataac cagtatggtt tatccaatcc ttatctatat cccaagctaa
    22261 tgatcttgat ttactcaaac tataattatt ccacaaaggc ttcgcaaaat catcttttaa
    22321 ataatgaata gaaaatcttt gtaaatcatc aatagttgaa aataaacctg cagaaccagt
    22381 atgctttcct aataccttag ctttaggatc gtgtacaata ccatcgttaa taccggctac
    22441 agttggaaca gcttctactc taggaccaaa agaggtttct ttcatctgaa aaggagtgaa
    22501 aatataacgt ttgaaaagtt tatccaaact atcccctaat acctcttcta acataaaacc
    22561 aagtaaaaga aaattaatat cagtgtattt aaacgattta tcttccaaaa ccttgatatg
    22621 attaatggca tctttcagct gagtagcatt caactgatca cgatttggaa taaaagggtc
    22681 aattccactg ctatgtgtag ctaattgcct tacagttaac gtctgatgat gaaacgtagg
    22741 gtaataatac cttaatggct tatcaatatc aatagtccct tgataaacta actttgctag
    22801 taaagttccc acgccgacaa cttttgatac actggctaga tcatagacca atcccgcttt
    22861 aactttctca ttgccatcaa ttgtccccaa gtaatgttcg tgccactctc cattttcata
    22921 aatagctaag cttgcaccat gataaagatt atctttgatt tgctccttaa tgagttctaa
    22981 aacggtatta gttttaatca ttcttttgaa atccaaatct caatattact agtgtcagag
    23041 atgactaaaa tcttttcttt agaatcaagg taaacatcaa gtccccatga ttcaaaagtt
    23101 gatttcaagt catttaaatt agtatcttca ttaacacagc attctagtat ctcaatatcc
    23161 caagtttcat ttggagcaat ttgcaaatct tgtccctgtg cttctttgaa agataaatta
    23221 attgggaact tcccagcaaa tactttatcg taaaaagctt cagctttcac ggcatcaggt
    23281 acattcaaac taatagattg gaaagcaaag tttgatagac ctgtagtgtc atcttttttt
    23341 tctaactctg ggcgctcaat tgccactaat tgtgacaaat catcttcagc atgaagtaag
    23401 aaaaggtctc cctcgggcga aactgtttca taggcataac cattttgacc ttgatatact
    23461 tgaatggctt gcgcaccatt tgccaaaagc ttttcaatat cttttgcatc ttgagactta
    23521 acaataatct ttgctaactt cttagtacca ttcaccgcac gagtacgata agttggggat
    23581 tcctcaataa taaagctagc ctctttgttc tgccatgctg agaaaactgc gattgcattt
    23641 tcttcactta tcaatttaaa tccaagagac tcctgataaa atgcaatatt caagtctcta
    23701 ttattaacac gtaatactgg gtgtttaaat gtcaatgaat gaaataacgt cataaattcc
    23761 tccttgttca gtctagtcta ttttagataa ttttagataa tttagcaaga atctttgtgc
    23821 tctcaatacc ataaagtcaa attgacagtt tcactatttt tttgtagact ggatattatg
    23881 aatgaaacac tactattaca tgggattcag cttattctga ttatcgccat gattatcact
    23941 ttttatcaga ttgttagaca tattaggtct caaaaaataa atcctttcaa acgatttttc
    24001 actggtttgt ggatcggttt tgtaactgat gctctcgata ctcttggtat cggctctttt
    24061 gcaacaacga ccacattttt taaactaact aagcttgttg aagacgatcg taagataccc
    24121 gcaacaatga cagctgcaca cgtacttcca gttttgttgc agtcactctg ttttattttt
    24181 gttgtaaaag tagaggcttt aacactaata acaatggctg gtgcagcttt tattggggct
    24241 tttgttggag ctaaaatgac taaaaattgg cacgctccta ctgttcaacg tattctagga
    24301 acactgctca tcacagctgc tattattatg ctctatcgta tgatcactaa ccctggcgct
    24361 ggtatttcag attcagtgca tggtctacac ggcatttggt tatttgttgg tatcgtcttc
    24421 aattttatta ttggtgtatt aatgactatg ggactaggta attatgctcc cgaacttatc
    24481 ttcttctccc taatgggact gagtcctgca gttgctatgc ctgtcatgat gctagatgca
    24541 gctatgataa tgacagcttc tagtacacaa tttatcaaga gtggtcgtgt taattggaac
    24601 ggctttgccg gtctggttac tgggggaata ttaggcgtta ttgtcgctgt tctcttctta
    24661 actaaccttg atcttaacag cttgaaaaca ctggttgtag gtattgtttt atttactgga
    24721 gcaatgttaa tacgttcttc ctttaaaaaa actatgccca aaagtcatca ttaaacgaaa
    24781 tcagctagca agactggctg atttttatat ttaaaacaaa aaaagaccgg taaaccgatc
    24841 tttctttaac tattaaagat ataatagttt gaacaaggct acagcgagta tacttgctac
    24901 gattggtgct aaaactggca cccatgcata ccaccatttt gaatcttctt tagcttgtcc
    24961 aagaatttgt tttggtaata agcggtgaac aatacgtgga ccaaggtcac gcgcagggtt
    25021 caatgcaggt ccagtcgatc cacctaaaga agcaactaga gtcattacca agaaaccaat
    25081 acctaagtga gctacagcaa gtgaaccagt tacgtatggt gaaatccttg tcgctgctgt
    25141 cgtttggtca taaccagctt gaacaagttt accaacaagt tctacaccaa aatagttctt
    25201 cgttaaagcc aaagctccaa agaaaagaac aaaagaacca acgaattcat ttaagaaacc
    25261 attaatgtaa gccgctttac gacttgattt ctgtccatca tctaatgcag aaatagttga
    25321 aaatgatcca agaacatgat ttggattttc tgtcttaaca aagtaaggtt gataaaccat
    25381 aacgacaact aactgtccaa atattgctcc taaaatttgt gcaagaatgt attgtccaac
    25441 gtgtgcccaa gggaaaagac ctgaaaatgc caatccaagt gtgaaagctg ggttaatatg
    25501 gtttccagag acattaccaa acataagcgc tggcatcata acacctaaac cataaccaat
    25561 tgcaataata atccaacctg aattatttcc ttttgtgcct tttaagtcaa cgttagctac
    25621 agccccattt cctaagatga taagaaaagc ggttgcgata aattctgtga tatacttcac
    25681 agtccaagtg atttcgatca atccttgttt cctccccaaa aaatatttac aacattaacc
    25741 attctaccaa gtaagcttaa tattgtaaag cttatcacaa aataaaatta ataaaatttt
    25801 taaccaaata tatttgataa aaaatagagt actaggttcg gactacctag tactctattt
    25861 tatttagttt ataaggtttt cttactatca cgaacgacta gcatatcaac tttcgaatga
    25921 cgtaaaatgt attctgaagt tgagccgatc aataggcttt caaaagtgtt taatcctgtt
    25981 gcgcctacca taattaaatc tgctttttcc cttatgggga tatcctttgc caaaagagtc
    26041 ttaggatttc cgatttcagt aattttttta atctttgtaa ggccttttga atgtgctaat
    26101 ttctcaaaat aggctagcac atctgctgac tcttgttctt gcctatcaaa aacatagtca
    26161 ctaaagactc cctcactttg gtaagcgtga gcatctataa catgcgttag cagtaattca
    26221 gcatcattac gaagagcaac atttatccct ttttctaccg ccaattctga ctcgtaagac
    26281 ccatcaatag caataagaat acgctcatat ttttgtgtca tagcttaatt cctccctttt
    26341 atatagtttt agtttcaact tgtctagatg attttaagaa atatagaata ccacaaacaa
    26401 caggaataat cgctgctatc cagaataatt ctctaaagga ttgcacacca gctccaagaa
    26461 aaccatcttt aacaagtccc aaaatgtaag gtccagcacc taaccctaaa tcaagaccta
    26521 tcatgtaagt tgacatggct gtattgaaac gatgttcctc aacacctttg attgatgctg
    26581 cttggccaca agacataaag gtgccataac caaaaccaat caaagcacct gatagaaggt
    26641 aggtaacact tcccatagca ctccctaata aagcaagtcc caaagttaag aacagataac
    26701 ttggataaag cacccactta tctcccttag cgtccattaa tcttcccata gatggtctgg
    26761 ttaaagtgat gacaagtgca taaacaataa agaaataagc tcctactgtc atcaaattaa
    26821 tttctgttgt atataatttt tggaaaccta acacggaagc ataggagata cccatcaaaa
    26881 atgcaataat tgtgataaaa attgcttttt tctcaatgaa actatcaata gtccatgatt
    26941 ttgatttagc taactgttct ggatttaaag taatattttt gactgggaaa acaaatgctc
    27001 ccaacactac aatcgcaatt aaaatactac ataatacaat aaccatttta aagttaatat
    27061 gaaggttgtc tagcataaat gttcctacaa aaggaccaat agctgcggct aaacttgttg
    27121 atagaccgta aaagttaatc ccctcacctc ttttatcagc tggtatatag gctgttacaa
    27181 tagtattagt tgctgttgac acgacgccat aaccaaaacc atttaggaaa cgaactaaat
    27241 acattactcc gatacttggc atataaaaat aagctaaagt tgttagtaag taaaaaatag
    27301 cccctccacg taaaactaac ttacgtccta gaacttctaa ttgcttacca aatataagac
    27361 gagcaatcaa agtccctaca atataaatcc ccgttgctaa tcctgcttgg ctagtgctaa
    27421 cacctaactc tttagtcgca ataaaagcta tgataacggt aaacaaataa tagaccatat
    27481 aaacaataaa gtttaaaata gtaatgccta taaaatgttt gttgaataat ttgtcttcca
    27541 atgcaaatgg ctcctttttt caatttcatt ttcataaaca ttttacaaaa atattagaag
    27601 atacgatatt tcaatggtat aatataagtg ctagtatcag actgcattaa caaatgttaa
    27661 ggtggtctat caaacctaaa acatttggag gaagtcatga atcatcaaat tttacaaaat
    27721 tacattaata gtcataatct tccaattatc gaaaaagact accataaata ccttacattt
    27781 gaaagtttag aagaagacta tacttatatc ctaaaagatg gtattgttaa acaaagtgtt
    27841 ctctctaaat atggtactga gttcaatctg cgttatgtga caggcttaga aatcacttct
    27901 atcctaaata cagactactc ccaacacatg ggagaacctt acaacgttcg aatcgaatcc
    27961 gaaactgccc acttttacaa agtacgtcgc tctaccttcc taaaagatat caataatgat
    28021 attgaactgc aaggatatgt gaaagatttc tatcataacc gccttgaaaa atctatgaaa
    28081 aaaatgcaat gtatgctcac taatggtcga attggtgcta tctccacaca attatatgac
    28141 ctcagtaaga tgtttggtga agaacgagat aacggtgata tctatattaa ttttgttatc
    28201 acgaacgaag aacttggtaa attttgcgga atatccacag gaagtagcgt gagccgcatt
    28261 ttgaaacaac taaaagacga tcatattatt cgtattgaaa aacaacatat cattattaca
    28321 aatgttgaga aattaaaaga tcatattgta ttttaatata acaatagaaa ggatatatta
    28381 tgcgttataa tcaattcagc tacattccaa ctaaacctaa cgaagctttt gaagagctca
    28441 aaggactagg ttttccatta aacaaaaaga attctgataa agctaatttg gaagcttttc
    28501 tcagacattc ttttttaaat caaactgata ctgattacgc tcttagtctc cttatcgttg
    28561 atgcaaaaac cgatgctcta acctttttta aatcaaatag tgacttaaca ctagaaaatt
    28621 tacaatggat ttatttacag ttattaggct ttatcccttt tgtagacttt aaagacccta
    28681 aagcattctt acaagatatt aacttcccag tctcatatga caatattttt caaagtctac
    28741 atcacttact cgcctgtcgt ggaaaatctg gcaatacatt aatcgaccaa ttagttgctg
    28801 atggtttact tcatgcagat aatcactacc attttttcaa tgggaagtct ctggccactt
    28861 tcaatactaa ccaattgatt cgcgaagttg tctatgttga aacatcctta gatactatgt
    28921 ctagtggtga acatgattta gtaaaagtta acattatcag acccactacc gagcatacta
    28981 tccctacgat gatgacagct agcccctatc atcaaggtat caatgatcct gccgcagacc
    29041 aaaaaacata ccaaatggag ggtgcgctag cagttaaaca acctaaacac atacaagttg
    29101 acacaaaacc atttaaagaa gaagtaaaac atccttcaaa attacccatc agccctgcaa
    29161 ctgaaagctt cacacacatt gacagttata gtctcaatga ctattttctt tctcggggtt
    29221 ttgctaatat atacgtttca ggtgtgggta ctgctggctc tacgggtttc atgaccagtg
    29281 gggattacca acaaatacaa agctttaaag cagtcattga ttggttaaat ggtaaggtta
    29341 ctgcattcac aagtcataaa cgagataaac aagtcaaggc taattggtca aatggccttg
    29401 tagcaaccac aggtaaatct tatcttggta ccatgtcaac tggtttagca acaactggcg
    29461 ttgaggggct gaaagtcatt atcgctgaag ccgcaatctc cacatggtat gattattatc
    29521 gagaaaatgg gcttgtgtgt agtccaggcg gctaccccgg tgaagattta gacgttttaa
    29581 cagaattaac atactcacga aacctcttag ctggtgatta catcaaaaac aacgattgct
    29641 atcaagcatt gttaaatgaa caatcaaaag caattgaccg tcaaagtggg gattacaacc
    29701 aatactggca tgaccgtaat tacctaactc acgtcaataa tgtcaaaagt cgagtagttt
    29761 acactcatgg actacaggat tggaatgtta agccaagaca tgtctataaa gttttcaatg
    29821 cattgcctca aaccatcaaa aaacaccttt ttttacatca aggtcaacat gtgtatatgc
    29881 ataattggca gtcgattgat tttcgtgaaa gcatgaatgc cttactaagc caagaactac
    29941 ttggcattga caatcatttc caattagaag aggtcatttg gcaagataat actactgagc
    30001 aaacttggca agttttagat gctttcggag gaaaccatca agagcaaatt ggtttaggtg
    30061 atagtaaaaa acttattgat aaccattatg acaaagaagc ctttgatact tattgtaaag
    30121 acttcaatgt gttcaaaaat gatcttttca agggaaataa taaaaccaat caaatcacta
    30181 ttaatcttcc tctaaagaaa aattatctcc tgaatggaca gtgcaaactc catctacgtg
    30241 ttaaaactag tgacaaaaag gccattttat cagcccaaat cttagactat ggtcctaaaa
    30301 aacgattcaa agatacacca accatcacat tcttaaacag ccttgataat ggtaaaaatt
    30361 ttgccagaga agctttacgt gaactcccgt ttactaaaga tcattatcgt gtcatcagta
    30421 aaggtgtctt gaaccttcaa aatcgtacag acttacttac aattgaggct atcgagccag
    30481 aacaatggtt tgatatcgag tttagcctcc aaccaagtat atatcaattg agtaaaggtg
    30541 ataatctaag gattatcctt tatacaactg attttgaaca taccattcga gataatgcta
    30601 gttactctat aacagtagat ttaagtcaat cttatttaac tatcccaact aatcaaggaa
    30661 attaacttat gaaacttctc actaaagaac ggtttgatga ttctcaacac ttttggtacc
    30721 agatcaattt attacaagag agtaacttcg gagcagtttt tgaccatgat aataaaaaca
    30781 ttccacaggt tgttgcaact attgttgatg atttacaagg ttccggaagt tcgaattatt
    30841 tctggtattt tggcaatact actgatactt ccatccttat gattgctcat ttaaatcgaa
    30901 aattctatat tcaggttaat ttaaagaact ttgactttgc actcaattta atagctataa
    30961 ataattggaa gagtctcctc caaactcaac tcgaagctct aaacgatacc ctagcaatat
    31021 ttcaataaat aaggtagaat ggagtgacaa agcaacgcga gggagactga ttaatgtcat
    31081 cttattggaa taactatcct gaacttaaaa aaaatattga tgaaaccaat caactaattc
    31141 aagaaagaat acaggtcaga aataaagata ttgaagcggc gctaagccaa ctcacagctg
    31201 cgggaggaaa acagctcaga ccagcattct tttacctttt ttctcaactt ggtaataagg
    31261 agaatcaaga tactcagcaa ctaaagaaaa tcgctgcttc tttagaaatc cttcacgttg
    31321 ctacattaat ccatgatgat gtcattgatg actcaccact aagacgtgga aatatgacca
    31381 ttcaaagcaa gtttggcaaa gacatcgcag tttacactgg ggatttactt ttcacagtct
    31441 ttttcgatct tattttagaa tctatggctg atacaccatt tatgaggatt aatgcaaaat
    31501 ctatgcgtaa aattctcatg ggagaattgg accagatgca ccttcgttac aatcaacaac
    31561 aaggtatcca tcactattta cgtgcgattt caggtaagac agccgaactc tttaaattag
    31621 ctagcaaaga aggagcttac tttggtggtg cagagaagga ggttgttcgt ctagcaggcc
    31681 atatcggatt taacattggt atgacattcc aaattttgga tgatatcctg gattatactg
    31741 cagataaaaa aacatttaac aagcctgtct tagaggattt agcacaaggc atttacagcc
    31801 ttcctctact tcttgccatt gaagaaaatc ctgatatttt caaacctatt ttagataaaa
    31861 aaacagatat ggctactgaa gacatggaaa aaattgctta tctcgtcgtt tcccatagag
    31921 gtgttgacaa agctcgccat ctagctcgta aatttactga aaaagctatt agtgacataa
    31981 ataagctacc ccagagctct gcaaaaaaac agttgctaca attaactaat taccttttaa
    32041 aacgcaaaat ttaaataata aaaaaacatt ccacaatgct agaaaagcag ttagggaatg
    32101 tttttttatt atcatttatt tatcgcacct atcaatcatc atagatcacc atcatcagcg
    32161 gctttcagct gacggtaacg ttggctactt tgagacaatt cttgaggaga accttccaac
    32221 tctaattgcc cattttctat aaataagata cgatcagcat gttcaatacc ttttaagtga
    32281 tgtgtaatcc aaactaaggt cttaccttcc aattctttca taaataccct tagtaaggct
    32341 tgttcagtaa taggatcaag tccaacagtt ggctcatcta agataacaat tgggacatct
    32401 tttagtaaga ttctagccaa agcaattcta tgcctttcgc cacctgaaaa cctaagtcca
    32461 gcttcatcaa ccattgtata gagaccatct gataaatcag tgaccatctc tttcaatcca
    32521 actcgttcaa gaactttcca tacatcttct tcactagcat cttggtttcc aatgcgaatg
    32581 ttatttagca gggttgtatt aaaaaggtag ggcgcttgtt gtatcactcc aatatagtta
    32641 gaaatgcaat caccaactat tgaaacatca gcaccgccta gggtaatctc cccttgactt
    32701 gctttcaagt cgccacgaag tagactggct aaggtactct tgccagaacc actacgccct
    32761 aaaatagcaa ttttttctcc ttctttaata tccaaatcta aatgatgcaa aacccatttc
    32821 tcttgtggct tatactggaa acttaaattc ttgacggaaa aatcgtatgg cttattaggc
    32881 aactgatttt gagttgtttc aaaatacgtt tctgataatt cattaagacg atgaattgaa
    32941 tcagaatatt tatttgtttc ttgagcagca gcagacaaac cagcaaatgc ctctgacaag
    33001 ggaaatactg ttagaacaaa tgctgcaatc cagttagctt cgccacctct gtgaccaata
    33061 aactgattac tagcccaaat aataaccaaa atagctagaa agccaaaaac taattccaca
    33121 ataagagctc tacgattatc aaaggaacga atttttttct gaacagccat gagttcactt
    33181 tccgaacgct catgcaaggc gacgtactct tgaccacgtt gactaaaaat ccagtctgaa
    33241 atacctaaga cattatccgt caaatcagta taaagatgat tttttaattc tttttcacga
    33301 gtctgacgag caccattagc taaaatcgac caaagtggaa aaagaaaaat cataatagct
    33361 aaataaagca acatcattaa agcaaaccat agagaaaaga agcctgttgc aataataata
    33421 aagctatata atatccacgc tataatcgtt ggaaatattg tcctaagata caaattttgc
    33481 aaataattaa tatcctccgc taaaagcccc ataacatctc ctaaacgaaa atcacgcttc
    33541 ataaagatag cattgctttc taaggaatgg taaagcttca agcgcagctg tgaagtcatt
    33601 cgtaataccc aattgtgact cgttaggcgt tctatataac gaaaaacagg acgtccaatg
    33661 ccaaaagcac gtgttagtac aattggaaca tatactagaa gaatattact cggtagtgaa
    33721 gctgacttac taatcagata gcccgaatta aacataagag cactggcaga aaagaaagtc
    33781 ataaaaccaa ggaaaagagc aattactaag gaaactttat attgtttgaa aaatggtttt
    33841 atccattgat ctgtctttaa ttctttaaac aaagggattt taaacatcat ttcctcccat
    33901 agcatgcttc agttgataga gatatccttt tttagctaat aattcctgat aagagcctac
    33961 ttctgcaact ctccccttct ccataacgac tataacatcc atttggttca accagtgaag
    34021 acgatgagtt gcaaagataa ctaaacgatc tgacatcagt ggcaacattt tctcttttaa
    34081 ttctaactct gtttcaatat ctaaatgagc agttggttca tcaaaaacca taatacgacg
    34141 attttgatca agaaaagcac gagccagagc tatacgctgg gcttgtcctc cactaagcgg
    34201 acgcgctcca ttaccaatac gcgtttctaa accgtcaggt aactcgctta gcaagctatc
    34261 taaacctacc atatgaatcg ctcttaccac ctcttcgtca ctagcattgg gagtatagaa
    34321 ggtaatatta tcacgtaatg acatttcaaa aacataagga gtttgaggaa tatagatcat
    34381 ctgtttatgc caatcttctt gatcaaggtt agtcacttct ctaccatcca cttttaaaga
    34441 accattatca ggccctaaaa aaccactcag aaggttaatt aagctagact tacctgaacc
    34501 actaactcct actaaagcaa ccttttgatg tcctctaaat cgtaaggtta catcctcaac
    34561 aacacgtttt tggtcgtacg caatagcaat gttttctaaa tccaaacgac tctctttaga
    34621 ccaatcatct atgactaatt gctcaccttt aataccagtt ttgtttagaa ctttttgaat
    34681 cgcttgaaat gcatttttcc cgtctaacgt tgcatgataa tcactagaaa aatctcttac
    34741 gggtaaaaag tattcagggg ataaaatcaa aattgttaaa gccggtaaaa ggtaaatctg
    34801 ctcatttaat aatcgtaaac ctagaaaaac agcaacgata gcaatggata aagttgtaaa
    34861 aaagtctaaa gcaaacgttg ataaaatacc aattttcaaa gtactcattg ttgctttacg
    34921 aaaagattca ctagtttgat aaatgctttt accataacgt ttactaagac caaaatattt
    34981 gagagtatca atgcctcgta atgaatctaa aaaatgatta gaaagtactt gataggattc
    35041 atattgttta tctgctttag cttgagccgc atagcctaaa ataatcataa agatgataat
    35101 taaaggaaaa acgataagta aaattgcacc tgattcaata tctaaataga aaatataagc
    35161 tagaataatc catgggataa tagacatgtt aatcatctta ttcaagacta aacgtaaata
    35221 attttcaact aaagaaacgc catctaatgc cattgtaaca acattaccag ttccttgttc
    35281 ttgaacgact ttagaaccta gatcaaataa cttatctaat aattgacgtc tcaaaagact
    35341 agattgtgct gttgaaaagt catcaagttt tctatttttg atgtaatcaa taacatgacg
    35401 gcctaaatat gagaccataa ataacaaaac ataaaccgtt tgagaagaaa gtttttgccc
    35461 ttcccacaaa ccagttatag aaagactcaa ataataagct tgtccgataa taaagattgc
    35521 ttgaagaaca tcaagtcccg caagcaatcc taacaactta tgaatacctg acaagcgcat
    35581 aaccgcttta tctagcatta tttttctcct gtcacaatca ctggcaaggt aatacgtttt
    35641 ctaaagatat aatatgccca tgctgtataa gctagaacaa agggaactag agtaattgcg
    35701 acaatcgaca ttacttttaa agtatatggc gttgaggatg cattttgaat cagcaaatca
    35761 tatttagggc taattgatga aatcataact cgtgggaata gaccttggaa caacagaaca
    35821 accactgaaa caagactgag accactcgct aaaaatgctg tcatctctgc tcctttaaaa
    35881 gttgaagcat gcgctaatac agctaataca acaataacca aaaccataat tgtcgtaact
    35941 atcggatgat tgctaaagaa atctgtcttg aaatacaaga gaactgcaaa caaggctaaa
    36001 ccaagataga gtacccaata aagcaactga gcataattac gggcacgctc tctaattggc
    36061 ccttctgtct tcaatgcaat gtaatttaaa ccatgaagat aggctagcaa taccattgca
    36121 actccaccca ccaacgagaa tatattaaag taatcactga attgagctga taagttacca
    36181 ctggcatcaa gtggcatacc ttgaatcaaa cttataaaca taatcccaaa gaagaatgga
    36241 acaatagcgg aaccaattgt taaggtccag ttccaaaact gctttttctc tgcaggaact
    36301 ttatgtctaa attcgaagga aacaccacga ataattaatc caaagagaat cgtaaggaga
    36361 atcaaatagt aaccactaaa tagtgaagca taccagtatg gaaaagaagc aaacattgct
    36421 ccaccaccgg taagaagcca aacttcatta ccatcccaaa ctgggccaat ggtttctact
    36481 acttgatctt tttcatgctc attatgtgtt aaagtttgaa cagccatccc aaccccaaag
    36541 tcgaatcctt caaggaagaa gaatcctgaa aataggaggc cgattaggaa aaaccaaaag
    36601 aattgtaaag cactcattta gtatgctcct ttctcaaagg gatcaattga agtatatgca
    36661 ccatgatgtc cgtgttcatc ttcatactct ggccctttct tcaattctct tattgttagg
    36721 cttatcatca tcaccgctaa accaccaaac aacaagaaat acacagtatt ggaaacaaat
    36781 aatgatgcaa ctgaaacgtt tggagagaca gagtccttaa tcttgaaaag accatagaca
    36841 gtccaagggt atcgtccttg ttctgtcaca atccaaccaa atgtattggc taagaatggt
    36901 gcaaaggttg ttaaagcaac aatccaaagc atccatttgt gtttataaag aattggtttt
    36961 ttcttacgag ttaaaaagag tcctaaaaca gaaacaccca taatagctgt tcccatcgcc
    37021 gccattgtac ggaaaccata gaaaagtagg ttaaccattg gatagtagtt atctttacca
    37081 tacttagcaa ccaattcttt attagctgta tccatacctt tcacagaacc agatggtttc
    37141 ccgtaagata aaatactcaa catataagga atcttaatcc caaaaacttg tttgtgttct
    37201 gcttcatttg cccaagcaac tacactccaa gctgcaggat cacccgaatc ttcataatca
    37261 ccttccatag cagcaaattt cattggttga tcgtgaatca aggctttcat ttgtaaatcc
    37321 ccaactccca taactgaaat tgaaccaaga agtgctacta ctaaaccaat acgaaccgat
    37381 ttcttataaa gttcaaccgt tgtatctttc agctgttctg attttaagag gcggaaggct
    37441 gacataccag caaccactgt accacccatc gtaatggcac caaaaataac atgggtaaat
    37501 tcatagaaga actgtggatt ttttacaagt gcaagaaaat cagtcatttg cgctctaccg
    37561 tttacaactt cataaccaac aggatgttgc atgaagctat tagcagtaag aatccacatc
    37621 gctgacatta atgagccaaa aaccactagc caaatgaatg tcacatgtaa ttttttacta
    37681 atttttttgt tatcccatgt aaacatccat aaaccaagga aagtagattc cataaagaaa
    37741 gctaataaag cttcaatagc caatggagct ccaaaaatat cacctacgaa acgtgaatag
    37801 tctgaccagt tcattccaaa ttggaactct tgaatgatac ctgtcacaac accaaccgca
    37861 aaactaagga gcatgatatt accccaaaat tttgtcaatt ttttatactc ttcatttttt
    37921 gtgataacat acattgtctc cataatagca accactaggc atgtaccaat tgtaaatggt
    37981 acaaagaaaa agtggaaaac tgtagtcatc gcaaattgaa aacgagctaa agtttcaatt
    38041 gtcatacaaa acctcttatt ttgttttaat catttctttt atagaaatat ctgcaagagc
    38101 tagtttccta gcttcgtaac aattggtaaa taacttactt taatatctta atgatataaa
    38161 tcaaaacgtc ccttagctag aagttcttta agtccaccca tatcaacaag tgatttgttc
    38221 atgataatct tcttcataac cgacgcaggg tattttttaa ttttagtttt tccaacaaca
    38281 ccaagtccat gcgtatttcc tactgaagca actgtgcctt gcggactata cgaaaagtct
    38341 tctgtagctt cacctttgat ttgatgtaaa aggtttttag caacatgagc tcccatacga
    38401 gttgcaattt gagctgttgt tgggaatggg cgaccagact cagtgtccat aaatgcagac
    38461 acatcaccga taacataaac attatcgtat tttggatcac gcaaatcact gttgaccatt
    38521 acacgtccac gacgttgatc aaatcctgat tctcccataa ccggacttcc actaacacct
    38581 gttgtccaaa tgatagtacc tgcggtaata gatttgagtt cttcatcctc tttactagta
    38641 ccataaacaa cttcacccgg tttgatttcc ttaatcatac ttccaagcat caaattaata
    38701 cctaaatcct taattaagtt gacaccatat tgcgctaatt catcatcaaa cattggtagg
    38761 atgcgagttg ctgcctctac acaaataatt tcaatttgtt caggttttac acccgctaaa
    38821 gcagcatatt ttggtcgctc atcaaccata gctccggcta actcaatacc tgtaaaacca
    38881 gcaccacata ctaagagttt gagaagattt tcatcttttg tctcacggta caacttcatt
    38941 gtattaacaa tgtgattatg gatgttttct gcttgcgaaa tattggtcat ctgcaaagca
    39001 ttctccatag cacccttgat gccaaaagtt tcagatacaa agcctaaggc aactacgaca
    39061 taatcataat ggagttcacc gttattttta actgttactg ttttattttc agcatcgact
    39121 ctaagcacct catcttgcat gaagttaacc ttattagtgt tgataacatc cttaattggg
    39181 aatgtaattt tttcttttgg ctgagagcct gcagctactt catgtaattc tgtagcttcg
    39241 tagtgataat cattcatgtc aacaagggta atatgaaaat cacctgattg tttttgtaaa
    39301 ttacgaactg ctttcaaacc ggcataacca gcacctaaaa ctaggatttc tttcatgata
    39361 aaactcctct aaaaaatgat tccaattaga tacgttaaga cttgggttgc agatcccagt
    39421 gctagaatac gtaccgcaca aacaaatgtt tctctcttaa cctgtttttg ccacaatagt
    39481 tttgtctgct tataaataaa tggtattaat aacaaactca agagtactgt aacaggcgca
    39541 agtccaaaaa gaaattcaaa aacaattgcc aataaagcta tactattaga aatagcaaac
    39601 agcaagagtc cacctcttat ccctgtataa tgtacaaggg tatagcgatg atttttttca
    39661 tcttcttctt tgtcgcacag attattcgcc aacattaggt tggcaatcca taaggtgttc
    39721 ggcaaggaaa tcaagaaaat tttgcttaag ttgaggatat cccacgaaaa gttttgataa
    39781 gtgtttagat atacacaaat taaactaatc ataaatccca ttgtcaagcc agaaaatact
    39841 tctcctaagg gcagactaga taaaggtctt ggaccaaagg aatataaaac accaattcca
    39901 aaacaaaaaa gtcccatcca taaaagaggc agaccgactt gggaaactaa aactaaacca
    39961 atcatactag aagtaatgac taaacttgcc ataataactt ctatttggcg tagggatagg
    40021 ttctcacgac caataatgtt agtgtcattc ttataatcca aatccacagc attccgataa
    40081 tcattgtaat tattccaaat gtcaacgaac atattgaata aaaacatagc aacgaagaat
    40141 aaaccgacat atacagggtg tactgaattg tagtaataag cactaaaaca caaaccaatc
    40201 aaaaatggta aaacactagc tgttttggct ttcatttcta ccaactctag aaagattggt
    40261 aatgtcatta ttaaagtcct ctctatacat aatcttcaac aatactatta taatgcaatt
    40321 tctaccattt ttaaaacaat agggcttgat ttttgattaa aaatacaaaa aatttttctt
    40381 aaaatgagtt aatcacactt agaacactaa atactatttt gaggagtcct attcatcaca
    40441 ctatgccatg taatattcgg aaaaatagta ttgcaaccct caactatccc ttatgttggc
    40501 accagccttt gaaagtcctc tgattgcact gcttagttag tattttcatc agctgtatga
    40561 tctctaagag ccatcagcat actaaaaaaa ctagtattac actttgaaaa gtgtaacact
    40621 agctccaatt tgttttaaat agcttaacta tttaatcatt aatctacttt atatatttaa
    40681 gcatttaaag ttgaatgttg gttgaggata cctgagagag aaccttgaat ataatttggc
    40741 aatacttgtt tttgagcacg ttcatagatt tctgaatctg ttaactgacg ttctgatttg
    40801 gggcttgata cttgcggtgt cactgctgca ttttgttgtt tgtaaagtgt tgaatgctgg
    40861 ttaaggatac ctgagagaga accttggata tattttggta atacttgttt ttgggcactt
    40921 tcataaagtt ctgaatctgt taactgacgt tctgatttgg ggcttgatac ttgcggtgtc
    40981 actgctgcat tttgttgttt gtaaagtgtt gaatgctggt taaggatacc tgagagagaa
    41041 ccttggatat atcttggcaa tacttgtttt tgggcacgtt cataaagttc tgaatctgtt
    41101 aactgacgtt ctgcttttgg aggtgttgct tgcggtgctg tgaaagttga agctgaaaca
    41161 ccttgtgtag aaccaagagc tacgaatgct cctccagcaa acattgctga gacaagacca
    41221 agtttcaaat agttagcagt tgagacgttg ttgacttttt tttctgtaat aagtttgttc
    41281 ataagaatta cctctttctt ttgggttatt tattttatga actaagtata acaacaagaa
    41341 ataaagtaag gttaaactaa atataaaata attcttaagt cattcttagg aaaccataaa
    41401 atcatacact aacaaaaaat ttggtatcta gctcaaaagt aaaagtagaa taaaggcttt
    41461 tacttctgag ctagtaatga ataaattata tttgcatcta gtttggaatt agccctagtg
    41521 tattataatg atccctacaa atatgtgcca aacaccttct tcatatatac aagattgatt
    41581 taacattcct atacccttaa tggtgggcac attctcttca tgttccatat agaaatcaac
    41641 agataattcc gtattgtccc tattattaaa ttttccagtt aatgataaat tatatcatcc
    41701 accttgaggt gtcctattac caatctgttc aacctttaca cttgacgaat cgaattgaac
    41761 agattctgtc ttatcattct gtgatttaga acatcaacaa tttcttgttc atgttctttg
    41821 aggtaagcta gttgttcttc tttaacgtgc ttttatcttt cgtacttact gatacttgag
    41881 agcatttttt tgaccctact tcttactaat cagctgacgt ccctataaga tgaatcataa
    41941 acacatagct gccactaacc attttaacct tttatgcata cataattctt cttgatatcc
    42001 cataccatta tctttagtat aaacacttaa taaaacaagc atttaacaac taaggttaat
    42061 aaaattcaat aaatctaatc cttctactta ctttagttta tcactttttt ctctcatcct
    42121 atgctataat agtaaggtat caaaatttag gagaatatag tcttatgatg aatatgcaaa
    42181 atatgatgcg tcaagcacaa aagcttcaaa aacaaatgga acaaacgcaa gctgatttag
    42241 cagctagcca gtttactgga aaatctgctc aagagctcgt taccgtgaca tttacaggcg
    42301 ataaaaagct aatttcaatt gattacaaag aagctgtggt agaccctgaa gatattgaaa
    42361 cactacaaga catgacaact caagctatca atgacgcttt aagccaagtt gacgatgcta
    42421 caaaaaaaat tatgggagca tttgcgggta aaatgccatt ctaaaaaata aatcacattt
    42481 actgaatcac ctgtaaatgt gatttttatt tcaaacttct ttcatttttt cacgaaaaca
    42541 agttttattt tcaaaaaata tttgcaaaaa atcagcagag aggtataata acattgaaaa
    42601 tattagagag aaggtgacta accatggtta tggatagtct aatcataaaa cagttgatta
    42661 agagtacatt tgatataccc ttacaagtca cttatccaaa tgggaatatt gaaacttata
    42721 atggtagcaa tccccacgtc aaacttaaac taaacaaaaa tttctccgtc tcggagctat
    42781 caaaagaccc ttccatagta ttgggtgaag ctgttatgga tggagacatt gaaatttatg
    42841 gttctataca agaattaatt ttatcagctt accgctgtgg agacagcttt ctacgtaata
    42901 gtaaatttag caaattgatt cctaaacaat ttcatgataa aaaacacagt aaatccgaca
    42961 ttcaaaagca ttatgacatc ggaaatgatt tctataaact gtggcttgat gataccatga
    43021 cttattcatg cgcctatttt aaacatgaaa atgatagtct agagcaagct caactaaaca
    43081 aagtacacca tattcttaat aaattaaatg ctcagccggg agggaaatta ctagacatcg
    43141 gttgtggctg ggggacactt atcattactg ctgctaaaga atatggctta aatgcgacag
    43201 gtatcacctt aagtgaagag caggcttctt tcattactaa gcgtatcaag gaagaagggt
    43261 tggaaaataa agtaacagtc ctcattaaag attatcgtga cattcgtgaa acatacgatt
    43321 atatcactag tgttggtatg tttgaacatg taggaaaaga aaatttatct caatacttcc
    43381 aaaccatttc taaaagactc aacatcaatg gtctagctct tattcatggt attactggac
    43441 aagtgggtgg taatcatggt agtggaacta attcttggat taataagtat atcttcccag
    43501 gaggatatat ccctcgttta actgaaaatt taaatcatat agctagtgct ggacttcaaa
    43561 ttgctgactt agaaccgctt cgtcgtcact atcaaaagac tctcgaatta tggacaaaaa
    43621 atttccacaa tgcccttcct gaagttcaga aaactcatga taagcgtttt atcaatatgt
    43681 gggacctcta tcttcaatct tgtgcggcct cttttgaatc tggcaatatt gatatttttc
    43741 aatacttgct ttcaaaaggt gtttcaaaag acactatgcc tatgacacgc gattatatgt
    43801 attctgctaa ttgatatagt taatattata gtattccaac ttattctaaa taaaaaggac
    43861 tctcttgaat tcaagggagt tctttaacgt tcttctctgg ctaactctat acaatgtgac
    43921 ttttggtgac aatgtggaca gaatactttt cgcgtcttag gggtatgttt cgctagatta
    43981 aatgcttgat atgtcggtgt aaaggtttga tgacaatttg gacaaagata aacaacctgt
    44041 tgtttaaaat accaaaccat accattcata gctattaaga atactgttac tgctatccac
    44101 ataagccatc tttgatttag agaaactgct gctattatta acgtaatata gaccataata
    44161 atcataacga cttgtagcac tatgcttttt tgtagtatac cccattttct tttgttttct
    44221 tccataagca caatgtcaga aagatagcct aatgttttac tatcttgttt ttctacagtt
    44281 tttagtaggt tgacagcttt gtcaagtttg tcttttttac tatcaatagc tagccttaat
    44341 tctcttatct acacttgtaa aaaaagctct aaaatctggc tagcattgtc ttcagtaaat
    44401 agatttttga tattatcaat tgaaaaatct aaatctcgta aataaataat ttgtctaagc
    44461 ttttctaaat cagcaatact ataaaccctc ctacctcctt cagatagttc tgatggggat
    44521 aaaattcctc ttttgtcata gtactgaaca gtacgaacag taactcctgc ttctttagct
    44581 aagtcaccag tggaatattt ttctgacatg tttcacacct cctttaatat agtaaacact
    44641 tctttatcag aaacaccttt actatatcaa atgacctaag gtaataagca agtaataacg
    44701 ctaagtattt ttaactttaa ataaaactac aacaattaat caaacaaatt tcctaaagtc
    44761 gcaaatgggc tatcaactgc tacctctttt tgctgtttta aatagatttt attttcatcc
    44821 aagtccagaa atttttcata aacacgcgca gtcatacggg catcctctaa gctattatgt
    44881 gctttaccct ttattcctaa aaagtcagca acagtagtca atttaaggtt gacaatacca
    44941 tttaaatctg tactgcgtct cacaaaagct tcatcatata agtccacctg atattgacta
    45001 gttagatcca aaccattttc aactaaaagg ggtaaatccg atttataccc attataacca
    45061 atcagtggtg tatctccaac aaaactctga aagtctgtca aaactatctc aattttagga
    45121 gcccctataa tatctctagc tgtaatacct gttaaaccat taataaagct ttgtaatgga
    45181 acttttgtgt gaacataagt atcaaataaa gcaatctctt gatgattaga atatttaacg
    45241 gccgacactt gaataatatg gctatgctca ccaaccgtgt taaactctaa atcaaaagct
    45301 atatattctt gtaattgttt cattatttct cctaaaaagc gcaaggaaac tccttacgct
    45361 tttattctat tttttattga agaaacgtcc aaagaaactt tttttaacag cttctaattc
    45421 ttgaattttt tcttctttgg cttcttgaat ctcctcaacc tctgctttag cttcatttaa
    45481 ctcaagttgc agacgttgcg tatcttccat agcagttaaa gtcaattgct gttgttgatc
    45541 taattgctta tctttctctg ctatttgaat atcttttaca tgtaactgct cgtcttttga
    45601 agcaatctga atatcttttg cttttaactg ttcatataaa cgtgtgattt cagtattctt
    45661 ctcatcaacg agaatttcta aaagttctcg tcttgatgct tcttcgtcaa ttggttcctc
    45721 ttccaaaata gtttttttat agatttcttc caatttaatc aagccatcac gattaacaac
    45781 tgtaacgcct ttactattct tttccacaca ttcttcaggg agtgttttaa ctctgttatt
    45841 catcgcttga cgactaaccc ctaaaatttc agctaattca ctaaccgtct tttcaactgt
    45901 cataacttcc tcttttgtac tgattttctt ataaaagaat atcacaatcc taatctagtg
    45961 tcaatttact gtcaacttaa agtgtcaaac aagacatttt gacgtctatt tctctattta
    46021 tatgctagaa taagattatg aagcattatg acacaataat tataggtggt ggacctgctg
    46081 gaatgatggc agctatttcc tcaaattttt acggcaacaa aacacttctt attgagaaaa
    46141 ataaacgttt aggcaaaaaa ctagctggaa caggtggtgg ccgctgtaat gttaccaata
    46201 atggaaacct tgatgagctc ttagctggta tacctggaaa tggccgtttc ctatatagcg
    46261 tcttctcaca atttgataac catgatatta ttaacttctt tcaagacaac ggcgtcacac
    46321 ttaaagaaga agatcatggg cgtatgttcc ctactactga taaatctcgt actatcatta
    46381 acgcccttga aaataaaata aaagaacttg gtggtcaaat tatgactgac acagaagttg
    46441 tctctgtcaa aaagatagga gattcattct atatcaaaac taaagataca cagttcgcat
    46501 ccgataaact tatcgtaaca actggtggta aatcctatcc gtctacaggt tctactggtt
    46561 tcggtcatga cattgctcgt cactttaaat tagaagtcac agatatggaa gccgccgaaa
    46621 gccctctttt aactgatttt ccccataaaa aactacaagg tatctcctta gatgatgtca
    46681 ctttgtcatt tgaaaagcat attattacac acgatcttct ctttactcat tttggattat
    46741 caggaccagc agccttgcga atttctagct ttgtcaaagg cggagaaact atatacctag
    46801 atgttttgcc caatatctct gttaaagaat tggaaataca cttccaaaat gaaagagaaa
    46861 aatctttgaa aaacgctctt aaaatacttc ttcctgagcg tttagctgaa ttttacgctg
    46921 aagacttacc cgaaaaagta aaacaagtat ctgtaaaaga cttagaaatg ttaattcaaa
    46981 aacttaaaaa acttcctatt ttagtaactg gcaagatgtc tctagcaaaa tcctttgtaa
    47041 caaaaggggg agttgatctt aaagaaataa accccaaaac acttgaaagt aaaaaggtag
    47101 ctggtttaca ttttgctggt gaagtcctag acatcaatgc tcatacggga gggtttaaca
    47161 ttacttctgc cttatgtaca ggctgggtag ctggtagctt acactattaa tacatgaaag
    47221 ctgaacaatt ccgtcagctt ttttatatag aatttaaaga taatatttgg ctcttaaggc
    47281 ttctttgatt ttttccaatt catctggtaa atttacctga tgaatagcat acccaatctg
    47341 ataaggggat actataaaac gaggaacaat tttacaaata agacccccat catgcttata
    47401 gaaaaagaga ttataagatt tacagcgccc tcccaagaat tcatttaaaa tataatgaca
    47461 caaaattggt aaactatctg caatcaaaat agcgttttcc aatttggata ctttaattgt
    47521 gaactctgtg aaatcaagca taggacgatt ggcaatacta atctctgctt gttgactcgt
    47581 ttccgaacaa acctcgatac catcaaacca atctaagttc aacgtttcat aagcatctac
    47641 gttttttaag ccaacaattt ggctatgagg atgtcgtaaa cttccgccag ataagggccc
    47701 aaaatttcga taaagaacga ccgaacggta atgaccactt tcttccatta attgccattt
    47761 tgacaaaaga taatctaaca attggcgcat ctttgactca ctataaactg aaatatcccc
    47821 attgtgatca tttgattcaa tcaccaatgt ttgataggtt tcttctaaag ttgggtattt
    47881 attttctacc cagatcatat cgtctttcac gtctaggatt tttcctaatt ggcttttatc
    47941 acaaaatgga catttgtctt tattgctggg tttttgtttc gcgatattag ggttaaatcg
    48001 gatatatctt tcattcatac tgttattgta acacattcat caccattaca atataatact
    48061 tatccagtta acattttcct tgcattctta gctgaaaaga gtataataga atcatgtctt
    48121 gtttaccggt taattaccag ttaactaggc accctgtttt gggatagaaa aatctttttg
    48181 ggaaaggaga aatcatggct aagaaatcaa aaattgctaa atttcaaaaa cagcaaaaac
    48241 tcgtagaaca atatgctgaa ctacgtcgtg agttaaaaga aaagggtgat tacgaagcac
    48301 ttcgtaaact tcctaaagat tctaacccta accgacttaa aaatcgtgat ttaatcgatg
    48361 gccgtcctca cgcttacatg cgtaaattcg gtatgagtcg tattaacttc cgtaacctag
    48421 cttacaaagg ccaaattcct ggcattaaaa aagcaagctg gtaattcgtg aagacaaata
    48481 gttacttttt atgactattc tattgagtgt ggacgaatag ttaatacatt gtaattattt
    48541 aacctgttga gtatttactc agctattaca aacttcttca aaattttatt atcataggaa
    48601 tgtaggatct catcaatcct taaatgacta ataataaaag atgaaaaaat ttttcttatc
    48661 tcaaatcata gatgccaatt tatttatttt caaaaagccc ggtatgccaa gataccgagc
    48721 tttttattat tataaatact gataaaattc ttttagttaa tcccaaaaac aagactatta
    48781 ttttgtaata gtcactactg aataatattt cttttattaa aaactcacaa gacatttctt
    48841 aaagcagtta catcttcgcg actagttgac caactagttg ctagacgtat tacagtatga
    48901 tggtcatcat atttttccca aaaactataa gctacattct ttgctaaatc tgctagtttt
    48961 gtattttcta caataataaa ttgttgatta gttggtgatt ttaaatagaa tgagtaacct
    49021 ttttcttcta aaatgatttt taattcctct gctaagtcga tcgcatgctt tccaattttt
    49081 aagtaaaggt tatccgtaaa gaaacgatca aaagcaagcc ccagcaatct tcccttggct
    49141 aataacgcac catgctgctt tacaattgta gtaaactgtt ttggacgatt tttctttgtg
    49201 aaaacaactg cttccccagc aagagccccc attttggttc caccaatata aaaaacatct
    49261 gataaggctg caatcgttgg gaaatcaaca tctgtatctt tcgcagccaa accatacccc
    49321 agacgagcac cgtcaataaa taaggggatt tgataatgtt tacaaatctt cgataactct
    49381 tctagttctg ctttactata aagtgttcca tattctgttg gatgtgatat ataaaccatt
    49441 cctggaaaaa ccatgtgttg gtaattacca tcagcataaa aagtctcaat ataagttgcg
    49501 acttcgcttg cgagtaattt cccattatga gaaggtaaag tcaatacctt atgccctgaa
    49561 aattctatgg ctcctgcttc atgactactc acatgacctg tttctgcagc aataactcct
    49621 tcataagaag caagcatact actgataact acttgatttg tttgagtacc acccactaaa
    49681 aactcaactt cagcttgtgg acaggaacag acttctcgaa ttttatctgc cgcttgttga
    49741 cagtaactat ctgtcccata tcccgataga ccttgatcat tagtttcaat aagggcattt
    49801 aaaagctctg gatgagctcc tttattataa tcattttcaa aatgtaacat tccttaactc
    49861 ctttattagt taaaaaaata gcgacaatat catttgctaa tatttttgaa tattcaatca
    49921 ttataacaaa taaaaaatat caaaacaagc gttttgatat tatgaacacc tataaactct
    49981 caaaagctaa actaggtttg gcattcaatt ttaaagaagc aaaattctct ttttcaaact
    50041 ctatcgctgc tgctaatgca atcattcctg cattatcccc acataatcta agaggaggaa
    50101 taaccacatc aatatcagga gaaatatcag tagccaagcg ttcccgaagt ccttgattag
    50161 ctgcaactcc accagcaacg actaaagttt tcactggata ctttaacaaa gctttttgag
    50221 ttttggccaa taaaatatcc aaaacagccg cctgaaagga agcacataaa tcttcaagaa
    50281 ccaatgcttc acccttttgt tctgcattat gatgtaaatt gataaatgca gattttaatc
    50341 cagaaaaaga aaattcaaga tgatcttctt tgatcatcgc tctaggaaaa tggtaagtat
    50401 cttgaccctt atgagctaac tgatcaatct cgcgacctgc tggataagtt aagcccataa
    50461 cacggcctac tttatcataa gcttctccaa cagcatcatc ccgagtttct cctactattt
    50521 tgtaatctcc cggttcagaa acatatacta attctgtatg tcccccactg acaagcaaag
    50581 ctaacaatgg gtattgaagt tccttaacgt cacgtgctgc cattaaatga cctgccatgt
    50641 ggtttatagg aattagaggt aatttatttg cccaagcgaa agcttttgct gcagccatac
    50701 ctaccaataa ggctcctact aatcccggac catatgttac agcaacagca tccaaatcgc
    50761 tagcaacaat acctgcttct tgaagagcat cctcaaaaca ggttgttact acttcaacgt
    50821 gatgacggct tgccacttca ggaacaacac caccaaaacg tttgtgactt tcaacttgac
    50881 ttgcaataat attagctagt aactctttat cattttttaa aatagcaaca ctagtttcat
    50941 cacatgaact ttccactgct aaaatatatc tatctttcat aatgatatta atccatcaat
    51001 atcgtctttt ccattagtat tgaggactat tcatatcctt tctatcatca actatttatc
    51061 ccttgcgcat cactatagca tcttcaattg gatcatggta ataatttttg cgctttctta
    51121 aaactacaaa accacacctc tcataaagtt tttgtgctac gatatttgaa taacgaacct
    51181 caagaaaaac tggcacctct atatcggcga tcattgacat taactggtaa gcataacctt
    51241 taccttgata atttttttta acagcaatat tagtaatttc tacttcacct accaattgtt
    51301 gaagagccaa aaaaccaatg acttcaccgt cattataaac aaaaaaataa tctgttgaat
    51361 ctttttttaa atcttctgaa atctgcgata aactccacgg agatttgtca tagacatcag
    51421 atagtacgct ccatatagcc agtgcttgtt ctgatgattc catgtcaaac tctcttaata
    51481 tattcttctg tattcgtttc acagtggttt tttaaccaat tttcctcagc ttcaacacgt
    51541 tttaagtatc gtggaacaaa cgcatctaca ttaacgcttt tcattttttg tcctttgcgc
    51601 ccaattgcta ctgcacaagg taaagtttct gtaattttag catgtggtaa ggctttctta
    51661 atctgatcaa aaaatgctgc aacctctccg acaaaatgaa cattggcttt attccccacc
    51721 tcttgtaaga cttcttcaag agaagtgtga cagtctggtt taacagtatc accattttga
    51781 tagaaaccaa cataaacatt gttacgtcgt gcatctataa gtggtaccaa taaatcattt
    51841 tctgaaaatc catttgttaa agcgtacagg ctagatactc caactaagtc aatcttaagc
    51901 gtataagcta gcatttttgc tgtagcaaca gctacacgta agcccgtata agatcctgga
    51961 ccctctgata ctacgatacg gtccaaatct tgaggttcta aatcaattga ttgcattaaa
    52021 aaatcaatgg ctggcatcaa attaatgcta tgattctttt tgatattgat agtgacagtc
    52081 gctaaacatt ccatattgtt tagtacagcc actgatagtg ctttgcttga agtatcaaag
    52141 gctaaaactt tcatcattta acttccctta cttgattcta cctcttattt tatgatataa
    52201 tcatgataat tgcaacgaat caaggttaat tctattactc tataaaaact taataatggt
    52261 ttgtcattgt ctcacttaac ggcattgacc cattttttta tagttagaaa aaccgagaga
    52321 taagaaagga ttattatgat ttataaagtt ttctatcaag aaacaaaaga acgcaaccca
    52381 cgtcgtgagc aaaccaaaac actttacgtt actattgatg cagctaatga attagaaggt
    52441 cgcattgctg cacgtaaact agttgaagaa aatacagctt acaatatcga attcatcgag
    52501 ttactttcag acaaacacct tgaatatgaa aaagaaactg gtgttttcga gctcactgag
    52561 ttctagaaag gagttaaaca taatagctag agtaatctac ccattatgat aaacttatta
    52621 tgtcaaatat caacttaaaa cccgaagaag ttggggttta tgcgattggt ggtctcggtg
    52681 aaatcggaaa aaatacttac ggtatcgaat atcaagacga aattattatt gtagacgctg
    52741 gtattaaatt ccctgaagat gatcttttgg gaatagatta tgttatccca gactattcat
    52801 atatcgttga aaacattgac cgtatcaagg cccttgttat aactcatggg cacgaggacc
    52861 acatcggtgg tattcctttt ctattaaaac aagctaattt accgatttat gcaggtcctc
    52921 tcgctcttgc tctaatcaaa ggtaagttag aagaacatgg tttacttcgt gacgctacat
    52981 tatacgaaat ccacgctaat acagagctga cttttaaaaa cttaagcgtt acatttttcc
    53041 gcactactca ctccattcct gaaccattag gtattgttat tcatacccct caaggtaagg
    53101 ttatttgtac aggtgacttc aaatttgact ttacacctgt tggggagccc gctgacttac
    53161 atcgtatggc tgctctaggt gaagatggtg tcctctgcct tttatcagac tcgacaaatg
    53221 ctgaagttcc aaccttcact aattccgaaa aaattgttgg gcaatctatt atgaagatta
    53281 tcgaaggtat cgagggacgt attattttcg cctcttttgc ttcaaacatc ttccgactcc
    53341 aacaggctgc tgaagctgct gttaaaactg gtcgtaaaat tgctgttttt ggacggtcaa
    53401 tggaaaaggc aattgttaac ggtattgaac ttggctatat taaagttcca aaaggtacct
    53461 tcattgagcc aagcgaactt aaaaatcttc atgctagtga agttttaatt atgtgtacag
    53521 gtagtcaagg tgagtctatg gcagcacttg ctcgtatcgc taatggtact catcgtcaag
    53581 ttactttgca gcctggtgat actgtgattt tctcatcaag cccaatccct ggtaatacaa
    53641 caagtgtcaa taaattaatc aataccattc aagaagctgg tgtcgatgtt attcatggta
    53701 aaattaacaa tatccatacc tccggccatg gtggtcaaca agaacaaaaa ttaatgttac
    53761 gcttgattaa accaaagtat ttcatgcccg tacacggtga gtatcgcatg caaaaagtcc
    53821 atgctggttt agcagttgac acaggtattc ctaaagagaa tatcttcatt atggaaaacg
    53881 gtgatgtttt agctttaacg agcgactctg ctcgtattgc tggacatttc aatgctcaag
    53941 atatctacgt tgatgggaat ggtattggcg atattggtgc ggcagttcta cgtgaccgcc
    54001 acgacttatc tgaagatggt gttgtccttg ccgttgctac cgttgatttc gattcaaaaa
    54061 tgattttagc tggaccagac atccttagcc gaggctttat ctatatgaga gaatctgggg
    54121 accttattcg cgaaagccaa catatcttat tcaatgctat tcgtattgca ctgaaaaata
    54181 aagatgcaag tatccaatca gtaaatggag ctattgttaa tgccttacgc ccattcttat
    54241 atgaaaaaac tgagcgtgag cctatcatta ttcctatggt ccttacccct gataaataaa
    54301 caaaagaacg gaaaatactt tctccgttct ttttaattgc ctatattagc aaaataagtt
    54361 aatatatcaa attacttctg ggataactat agaatataaa agaactccaa acagtatcgt
    54421 acttttatac cgtctatgtg aggagttctt tatagattaa ttcggataaa tgtagctaac
    54481 tacccctggt gttactgtat tttgtggatc aaaccaacca cgataattat caatccattg
    54541 atgacctgca taattagatt ctaaaacctg aattttatta gtgacaggat ctacagctgt
    54601 aacataagcc acatgtccat aactaccatc agtccaacat acgatagaac ccacgcgcgg
    54661 ttgtgtccct actgtataac cagctgatgc ggcgctcgca gcccaatcac caccgtttcc
    54721 ccaccagtta cctgcccatg ttgccatctc ctttacaccc catgtgcatt gacctagtgg
    54781 aaacgtatta ctagctggat gtgcttgagc aatttcctcc gcacttgcta ggtagtctac
    54841 atctactgca tagttatatg tattcgcatc tgcatttgtt gaagcaaatg aaaaagcgga
    54901 caccaaagcc actgttccaa gcattgtttt agactttaat tgttttatgt tcatgttttc
    54961 ctccatctga tgactcctag tttatcaaat ccatataaca gacaaatgaa aacctgtaac
    55021 aagttttagg caatttttta caataattta caaaaaaact agttttagac tttacaaaat
    55081 aaagcctaaa actagttata aacattctta tgattaatat aaatctaggt aattatcaat
    55141 ttcccattgt gaaacataag tcgcataact tgcccattca atacgcttag cgtctaagaa
    55201 attagtatag atatgagttc ctagggccgc ttttaccact tcatcttctt caagtgcttt
    55261 aagagcatta tgaagagtag atggtaaatc aacaatacct gcttgacgac gttcttctac
    55321 agtcatagca tagatatttg tctcaattgg ttctggtgct tcaattttat tctcgattcc
    55381 ctcaagtcct gatcccaaaa gtactgataa agcaagataa ggatttgctg ttgggtcaac
    55441 cgagcgcaat tctaggcgtg ttcccatacc tcgagatgca ggtacacgaa taagtggcga
    55501 acgattgcga cctgcccaag caatgtaaac tggtgcttcg tatcctggta ccaaacgttt
    55561 ataagaatta acagttggat taatgatagc tgtgtaattg taagcgtgtt tcattaaccc
    55621 acctaaaaag tagtaggcat cctctgataa ttgcattcca cgtggatctt ctggatcaaa
    55681 gaaagcattg ttcccttcat tatcaaaaag agacatatta caatgcatac ctgaaccatt
    55741 gataccaaac ttaggttttg ccataaaagt agcatataaa ccatgcttac gagcaattgt
    55801 tttaacaacc aatttaaata attgaatgtt gtcacaggct tttaaaacat catcatattt
    55861 aaagtcaatt tcatgctgtc caactgctac ttcatgatga ctagcttcaa cttcaaagcc
    55921 catttgtgtt aagacattaa caatttcacg acgtgtatta tctgctaagt ctgttggagc
    55981 taagtcaaag tagcctcctt tgtcattaac atcaagtgtt gggttaccat tttcatccat
    56041 tttaaatagg aaaaattcag gttctggtcc taagttaaat gacttatagc ccatctcttg
    56101 catacgtttc atattacgct taagatttcc acgtggatct ccggcaaaag gttctccttc
    56161 tgctgtatag atatcacaga ttagacctgc aacagctcca ttttcatctc cccaaggaaa
    56221 gacaatccaa gtatctaaat caggatataa atacatatca gattcattga tacgaacaaa
    56281 gccctcaata gaagatccat caaacattgc tttatttgat aaaactttgt ctaattgttc
    56341 atctgtagcc ggaatttcga catttttcat tactcctaag atatctgtga acatcaaacg
    56401 gaggaaggta acattttttt ccttaacttc gcgacgaata tcttctgctg tgatagtcat
    56461 atagtctcct taatatctag cttgactgca tgtttacaaa tgcatatttc caatgtgctg
    56521 tgaaggactt gaaaaacgcc cttgattacg gagctcatct tgtaaaagtc gacgaacctc
    56581 ggcatctgat aatgactttt gtttctcttt ggacttatgt tgtctttgca tgtattcatt
    56641 tttaatatca gaaatatgca aaccatcgct aatgaagtct tttatttcca gtaagcggtc
    56701 catatcattc agtgaaaaca tgcggcggtt accttcagta cgttcaggtg tgattaaccc
    56761 ttgatcctca taatagcgaa tttgtctggc agtcaaatct gtcaacttca taacagctcc
    56821 aatcgggaat acagccattg tacgcctaag ttctctttct ttcatgatgg cctcctttca
    56881 ttaaaccatt ataaacttta aaaagtttga tgtcaactct catgtgagat tttatgacat
    56941 caaactataa tttgttattt tttagttttt tccgaagtat ttcaatatct gtattaacca
    57001 tcattgcaat aaaaacacca caaaaagttt caaaaatacg tgcaaaaaca tatagaattg
    57061 tctctccgct tgggatagat aaagttataa taagtaatgc ggctgttccc ccaataatac
    57121 ctgatttatt attacaagca acattaatca tgatacataa catggtacaa ataggaacaa
    57181 ttaataaagt gacccaaaaa gcttgatgaa atatctcatt gaatgcaaaa aatactaagg
    57241 agagtaagcc acctatacta ttaccaataa ttctagaaaa accaaagtgc acgcttttat
    57301 caaagtcttc tcttaaacta aatacagcag ttagagcacc aatttgaagc ccgttccaac
    57361 caaataaatg aaaaaccaat aaaactaaaa agaccgataa gcctgtttta aatgttctca
    57421 ttccaaattt aaacttttta atatcaaact tgtaattaga taataatttt tttaacgttt
    57481 tcatacctat cccttcacta tatattctaa catatcaatc cataaaaccc tttttgtaaa
    57541 cgttttattt attactagta tatcttttat aaaccaattt atatcaaatc gtcataaact
//
LOCUS       DATRUK010000005        54756 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.005, whole genome shotgun sequence.
ACCESSION   DATRUK010000005 DATRUK010000000
VERSION     DATRUK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 54756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54756
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.005"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..714
                     /locus_tag="VBE34_000241"
     CDS             <1..714
                     /locus_tag="VBE34_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7262996.1"
                     /translation="YQDIMDHYFWLEKWLIRVDFDYMLNRLNVFELEKKSHNTGDKLL
                     SIKDFQVKLSKNKFISYLDFDLASGERLCLDGPSGVGKSSFFMGLLGLYRTKGKKQFT
                     HRKQIPISFLFQNPLDQFIFSTVYDEIFQVCKDSNKARDILETINLWDKKQFSPFQLS
                     QGQQRRLAIGSILASDSKLLLLDEPTYGQDAYHANMITTLLLSYCHKNHCGVIFTSHD
                     PHLKECFATRFLEVSSCSI"
     gene            702..1370
                     /locus_tag="VBE34_000242"
     CDS             702..1370
                     /locus_tag="VBE34_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEO7262997.1"
                     /translation="MFNLKNYHPAITFLCLLIPTFSFSFTLRLRTSLLFLIIVVTLQC
                     FVKVSLKTWAKVNLISFVMGLSLFLGTYFWGKLPHQFVLASLVACRPLIFMNVGLLFH
                     ASHSNYDFIESLYQTFKVPSHFAYGIFAVFNLLPLIKLQYQRNRLAFRLKNQVTWALS
                     PRLILSVLLKTIYWVEQLELAMLSKGFEVGKERTHASTYPVRFRDYSLLGMSILLAIG
                     MIFK"
     gene            1454..2134
                     /gene="tenA"
                     /locus_tag="VBE34_000243"
     CDS             1454..2134
                     /gene="tenA"
                     /locus_tag="VBE34_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEO7262998.1"
                     /translation="MLKDEMIAKGTKYIQSIYQDPFIQGIVKGRLDHDVICHYLQADN
                     IYLGKFADIYALCLAKSDNLRDKQFFLEQVDFTLNRELADGEGPHQALAAYTNRSYQD
                     IIEKAVWYPSADHYIKHMYFHFYENGIAGALAAMSPCPWIYHQLAKKIIEENQFLNGN
                     PFNNWITFYANDTVEELMENYFRMMDYYAQNLSKEKQADLVDAFVKSCQHERRFFQMA
                     INQEKWED"
     gene            2136..2933
                     /gene="thiD"
                     /locus_tag="VBE34_000244"
     CDS             2136..2933
                     /gene="thiD"
                     /locus_tag="VBE34_000244"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO7262999.1"
                     /translation="MTYLAPVLTIAGTDPSGGAGIMADLKTFQARRTYGMAVVTSVVA
                     QNTCGVRGVQHIETAIIDQQLACVYDDIKPKAVKTGMLAERETISLVASYLKKYPQPY
                     VLDPVMVATSGHRLIDSDAVEALKEDLLPLATIITPNLPEAEVLVGYDLSDEVSIIKA
                     GYDIQKQYSVRNVLIKGGHLDGLAKDYLFLEKEGLITLSNQRINTIHTHGTGCTFAAV
                     VAAELAKGQSILNAVSTAKSFITSAIETAPELGLGNGPVNHTSYQGD"
     gene            2935..3705
                     /gene="thiM"
                     /locus_tag="VBE34_000245"
     CDS             2935..3705
                     /gene="thiM"
                     /locus_tag="VBE34_000245"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEO7263000.1"
                     /translation="MYLEQLKEVNPLTICITNNVVKNFTANGLLALGASPAMSECIED
                     LEDLLKVADALLINIGTLTKESWQLYQEAIKIANKNQVPVVLDPVAAGASRFRLEVSL
                     DLLKNYPISLLRGNGSEIAALVGEKQASKGADGGKVADLESIAVKANQVFDVPVVVTG
                     ETDAIAVRGEVRLLQNGSPLMPLVTGTGCLLGAVLAAFIGSSDRSDDLACLTEAMTVY
                     NVAGEIAEKVAKGKGVGSFQVAFLDALSQMKSEMIMDK"
     gene            3719..4390
                     /locus_tag="VBE34_000246"
     CDS             3719..4390
                     /locus_tag="VBE34_000246"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001904425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEO7263001.1"
                     /translation="MKDTLKLYFVCGTVDCSRKNILTVVEEALQAGITLFQFREKGFT
                     ALQGKEKIAMAKQLQILCKQYQVPFIIDDDIDLVELIDADGLHIGQNDLPVDEARRRL
                     PDKIIGLSVSTMAEYQKSQLSVVDYIGIGPFNPTQSKADAKPAVGNRTTKAVREINQD
                     IPIVAIGGITSDFVHDIIESGADGIAVISAISKANHIVDATRQLRYEVEKALVNRQKH
                     SDVIK"
     gene            4517..5776
                     /locus_tag="VBE34_000247"
     CDS             4517..5776
                     /locus_tag="VBE34_000247"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO7263002.1"
                     /translation="MRKIIINGGKQLTGEVAVSGAKNSVVALIPATILADDVVVLDGV
                     PAISDVDSLVDIMETMGAKIKRYGETLEIDPRGVKDIPMPYGKINSLRASYYFYGSLL
                     GRYGQATLGLPGGCDLGPRPIDLHLKAFEAMGASVSYEGDSMRLATNGKPLQGANIYM
                     DTVSVGATINTMIAAAKANGRTVIENAAREPEIIDVATLLNNMGAHIRGAGTDVITIE
                     GVKSLHGTRHQVIPDRIEAGTYIAMAAAIGRGIKVTNVLYEHLESFIAKLDEMGVRMT
                     VEEDSIFVEEQEKLKAVSIKTSPYPGFATDLQQPLTPLLLTAEGNGSLLDTIYEKRVN
                     HVPELARMGANISTLGGKIVYSGPNQLSGAPVKATDLRAGAALVIAGLMAEGRTEITN
                     IEFILRGYSNIIEKLTSLGADIQLVEE"
     gene            5878..6432
                     /locus_tag="VBE34_000248"
     CDS             5878..6432
                     /locus_tag="VBE34_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7263003.1"
                     /translation="MDIWTNLGRFAFIETEHVNLRPVAYTDREAFWRIASKRTNLQFI
                     FPVQTSKKESDFLLVHSFMKEPLGVWAIEDKVSHKMFGVIRFENIDLSKKTAEIGYFL
                     KESSWGQGIMTECLKTLSFFAFQEFGMDKLIIVTHKENIASQKVALKAHFKQSRSFKG
                     SDRYTRRIRDYIEFQLTRGDYRYE"
     gene            6425..7708
                     /locus_tag="VBE34_000249"
     CDS             6425..7708
                     /locus_tag="VBE34_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO7263004.1"
                     /translation="MSKHQEILEYLENLAVGKRVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETRPRSGTVRVAQKAKVNIEKLTYAEIARISDSQVVAGTEGLSKEFSKFSIGA
                     MTHRNIEKYLVQGGLLIVGDRDEIQHLALQHQNAILVTGGFNVSPSVCRLADKLQIPV
                     MVTHYDTFTVSTMINHTLSNAKIRTDLKTVEQVYQSQMDYGFLAQDDTVKEFNLLVKQ
                     TKNVRFPIVNQANVVVGVVSVQDILGKDKEVKLATVMSKNIIVAKPRMSLANISQKMI
                     FEDLNMMPVVSDDFELLGVITRRQAVENLSMSQGTALYTYSDQILSNLQIEDGHFSFL
                     VEPAMIDHTGSLTQGVLTEFLKEICIRVLTRKHQRSIVVKQMTLYFLQPVQIDEIIMV
                     TPTIISEKRREATLDLELKLENKIIAKAMIAVKIN"
     gene            7731..8591
                     /locus_tag="VBE34_000250"
     CDS             7731..8591
                     /locus_tag="VBE34_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEO7263005.1"
                     /translation="MITLKSAREIEAMDRAGDFLASIHIGLRDIIKPGVDMWEVEEYV
                     RRRCKEENVLPLQIGVDGAVMDYPYATCCGLNDEVAHAFPRHYTLKQGDLLKVDMVLS
                     EPLDKSIVDVSSLNFDNVAQMKKYTETYSGGLADSCWAYAVGEVSQEVKDLMSVTREA
                     MYIGIEKAVIGNRIGDIGAAIQDYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLREGMVLTIEPMINTGTWEIDTDMKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            8593..9513
                     /locus_tag="VBE34_000251"
     CDS             8593..9513
                     /locus_tag="VBE34_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEO7263006.1"
                     /translation="MKLKKFFEDLLAKLEYRPIQVFMRHFQSAEMDLSAIAVAYYLLV
                     TAFPLLVIAANIFPYFHINVSDLLSLMQKNLPKNIYEPASRLAVDAFSKPSTGILGFA
                     SLTAFWTMSKSLTSLQKAINKAYGVDQHRDFVISRLVGVGTGLIILFLLTFVLIFSTF
                     SKPVLQIIVNMYDLGDTLTAWLLNLAQPVTFLTIFLGIGILYFILPNARIRKVRYVIP
                     GTLFSTFVIGFFSNLISQYVLNRVEKMVDIKTFGSVVIFILMLWFIFLAHIMILGAIL
                     NASVQEIATGKIESRRGDIMSLIQKSKEEK"
     gene            complement(9530..9985)
                     /locus_tag="VBE34_000252"
     CDS             complement(9530..9985)
                     /locus_tag="VBE34_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEO7263007.1"
                     /translation="MVKLKSLLKKSIQNEVSLYLLFGLLTSLLYLVIRQGIFNFSQDA
                     PFSAIVANIIAILFAFFTNDRFVFKQTKIGQFQRLQTFVIARLGTLGLDLILAVIFVD
                     QFPSIIGQFVQHNLNKINTIESLVSQILIILLNYILSKFVIFKDKKRQL"
     regulatory      10056..10169
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            10167..10676
                     /locus_tag="VBE34_000253"
     CDS             10167..10676
                     /locus_tag="VBE34_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEO7263008.1"
                     /translation="MNTFTTRDYAHMAIVTAIYIVLTITPPFNAIAYGAYQFRVSEML
                     NFLAFYHRKYLFAVTLGCMISNLYSFGMIDVFVGGGSTLLFVYLGTILFKQYQKDYLF
                     NGLINKAFFFFSFFFAASMITVAVELKIVAGLPLLLTWLTTAVGELTSLLVGAVLVDK
                     LSRHVDFTK"
     gene            10816..12774
                     /gene="ligA"
                     /locus_tag="VBE34_000254"
     CDS             10816..12774
                     /gene="ligA"
                     /locus_tag="VBE34_000254"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEO7263009.1"
                     /translation="MENRMNELVSLLNQYAKEYYTQDNPTVSDSQYDQLYRELVELEE
                     QHPENILPNSPTHRVGGLVLEGFEKYQHEYPLYSLQDAFSKEELIAFDKRVKAEFPTA
                     SYMAELKIDGLSVSLTYVNGILQVGATRGDGNIGENITENLKRVHDIPLHLDQSLDIT
                     VRGECYLPKESFEAINIEKRANGEQEFANPRNAAAGTLRQLNTGIVAKRKLATFLYQE
                     ASPTQKETQDDVLKELESYGFSVNHHRLISSSMEKIWDFIQTIEKDRISLPYDIDGIV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVVVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPNQIKERLAHFASRDAMNITGFGPSLVEKLFD
                     AHLIADVADIYRLSIEDLLTLDGIKEKSATKIYHAIQSSKENSAEKLLFGLGIRHVGS
                     KASRLLLEEFGNLRQLSQASQESIASIDGLGGVIAKSLHTFFEKEEVDKLLEELTSYN
                     VNFNYLGKRVSTDAQLSGLTVVLTGKLEKMTRNEAKEKLQNLGAKVTGSVSKKTDLIV
                     AGSDAGSKLTKAQDLGITIQDEDWLLNL"
     gene            12786..13805
                     /locus_tag="VBE34_000255"
     CDS             12786..13805
                     /locus_tag="VBE34_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO7263010.1"
                     /translation="MKKQKKARLIYNPTSGQEIMKKNVAEVLDILEGFGYETSAFQTT
                     PTKNSARDEAARAAQAGFDLIVAAGGDGTINEVVNGIAPLKRRPKMAIIPTGTTNDFA
                     RALKIPRGNPIEATKLIGKNQIVKMDIGQAQEDNYFINIAAAGSLTELTYSVPSQLKT
                     TFGYLAYLAKGVELLPRVRKVPVKITHDKGEFIGDASMIFVAITNSVGGFEQIAPDAK
                     LDDGKFTLILVKTANLIEIMHLIRLVLAGGKHINDKRVEYIKTSYLTIEPLSDERMMI
                     NLDGEYGGDAPITLANLKNHIRFFANTDEISDDALVLDKDELAIEAIAQKFANEVNDL
                     EGLGD"
     gene            13809..16109
                     /gene="pulA"
                     /locus_tag="VBE34_000256"
     CDS             13809..16109
                     /gene="pulA"
                     /locus_tag="VBE34_000256"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEO7263011.1"
                     /translation="MDNTVIVHYHSRKKNYFNLSLWQWRDGSEGQDAHFSRFDSFGAV
                     AILKYPLPYFLSHYYVIVKDKNWHHKTIDYRIDCTKGGARKEVWIVDGDDNLYYSRQA
                     AVSSHHYSRRQPHAYDMAINSRAFDKKWGFDGWLGFEYSETKTAFRLWAPTAERVELI
                     LYHSTDETASVSKVLSMKRGTAVNYKNHKENTHGVWFTELEGNYNYQAYTYRVYYRRR
                     TFKITRDPYSIATTANGKRSIVIAPEALTPEGFKISHGKEAKWRLENPNQAVIYEMHV
                     RDFSISETSGVKTDYQGKFKGLHQKDTVNQHGDKTTFDYVQDLGVNYIQLQPIFDHHQ
                     TFDDDGHYAYNWGYDPENYNVPEASFSSNPHEPATRILELKSAIQAYHDAGIGVIMDV
                     VYNHTFSSTDSAFQLTVPDYYYRMNHNGTFQNGSGCGNETASEKEMCRKYILDSVLYW
                     VKEYNIDGFRFDLMGLHDVETMNIIRNELNKIDPRILVYGEGWDMGAGLTPQNKAKKD
                     NAYQMPGIGFFNDDVRDAVKGAEIYGEFKKGLVSGNSTEDIVAKGILGSDELVSYIDP
                     SQVLNYVEAHDNYNLNDLLWELHPNDNEKQHIYRVEVASAMNLLMQGMAFMQLGQEFL
                     RTKCYPTGDKGQLTQADKERAMNSYNAPDQVNQVNWDNVTFHKSTINFIRKIITLKTN
                     SPYFSYSSFEEIRKHVFVESAQYHSGFISFTVEEHKKIKIIFNIKGKRLQDPFIGDII
                     ISNHEEGKAIDSLDDELSVMILDITK"
     gene            16314..18182
                     /gene="glgB"
                     /locus_tag="VBE34_000257"
     CDS             16314..18182
                     /gene="glgB"
                     /locus_tag="VBE34_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEO7263012.1"
                     /translation="MSNKDELYTFGIGENFHLQNYLGVHSENGSFCFRVWAPNAENVQ
                     VIGDFTDWRNRPLQMNKIQAGVWEANSLDAREGDLYKYLVTRKGGQVVEKIDPMAVYM
                     ERRPGTASVIKVLRNKKWEDGLWMGRRKRLGFQKRPINIYEVHAGSWKKDDFGHPMTF
                     SQLKDYLIPYLVEMNYTHVEFMPLMAHPLDMSWGYQLMGYFAFEHTYGTPEEFQDFVE
                     ACHKNNIGVLVDWVPGHFIQNDDALAYFDGTATYEYQNHDRAHNYRWGALNFDLGKNQ
                     VQSFLISSALFWIEHYHIDGIRVDAVSNMLYLDYDEGPWEANQFGDNRNLEGYHFLRK
                     LNKVIKERHPNVMMIAEESTASTPITKDLESGGLGFDFKWNMGWMNDILRFYEEDPLY
                     RQYDFNLVTFSFMYIFNENFVLAFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNLYAYQ
                     MCHPGKKLLFMGSEFGQFLEWKYNDQLEWENLNDDMNQKMQRYTKQLNQFYKDHKCLW
                     RIDDSFDGLEIIDADNKSETVLSFIRKDDKGDLLLCVFNMTPVERPNFTIGVPQAGIY
                     EEVLNTEMEEFGGVWKNHNPVTKTQVATWKDYDHTLSFTLPALGASVWRIKRRLRK"
     gene            18224..19363
                     /locus_tag="VBE34_000258"
     CDS             18224..19363
                     /locus_tag="VBE34_000258"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019785022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEO7263013.1"
                     /translation="MKNEMLALIFVGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGINNVGVITQYQPLELNTHIGNGSSWGLDGIDSGVTVLQPYSATEGNRWFQGT
                     SHAIYQNIDYIDRINPEYVLILSGDHIYKMDYDDMLQTHKDNLASLTVAVLDVPLKEA
                     SRFGIMNTDSNDRIVEFEEKPEHPKSTKASMGIYIFDWKRLRTVLIDGEKNGIDMSDF
                     GKNVIPAYLESGERVYTYNFDGYWKDVGTIESLWEANMEYIGEDNKLHSRDRSWKIYS
                     KNLIAPPNFMTEDANVKDSLVVDGCFVAGNVEHSILSTNVQVKPNAIIKDSFVMSGAT
                     IGEGAKINRAIIGEDAVIGDGVVIDGSKEVEVIGYKEVAGVPNED"
     gene            19353..20486
                     /gene="glgD"
                     /locus_tag="VBE34_000259"
                     /pseudo
     CDS             19353..20486
                     /gene="glgD"
                     /locus_tag="VBE34_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687083.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
     gene            20483..21913
                     /gene="glgA"
                     /locus_tag="VBE34_000260"
     CDS             20483..21913
                     /gene="glgA"
                     /locus_tag="VBE34_000260"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEO7263014.1"
                     /translation="MKIMFVAAEGAPFAKTGGLGDVIGALPKSLSKKGHDVAVVMPYY
                     DMVDQKFGDQIENLMYFYTDVGWRHQYVGVKRLSQDNVTFYFIDNQYYFYRGHVYGDW
                     DDGERFAYFQLAALELMEKIDFIPDVLHVHDYHTAMIPFLLKEKYHWIQAYNNIRAVF
                     TIHNIEFQGQFGPEMLGDLFGVGAERYEDGTLRWNNCLNWMKAAILYSDRVTTVSPSY
                     ANEIKTPEFGKGLDQIMRMEAGKLSGIVNGIDSDLLNPETDAFLPYHFSKSNLEGKIK
                     NKLALQENLGLPQDKNVPLIGIVSRLTDQKGFDIIASELDNMLQQDIQMVILGTGYHH
                     FEETFSYFASRYPEKLSANITFDLGLAQQIYAASDIFMMPSAFEPCGLSQMMAMRYGS
                     LPLVHEVGGLKDTVVAFNQFDGSGTGFSFNHFSGYWLMQTLKLALEVYNDYPEAWKKL
                     QWQAMSKDFSWDTACVAYEQLYQQLQ"
     gene            22245..22445
                     /locus_tag="VBE34_000261"
     CDS             22245..22445
                     /locus_tag="VBE34_000261"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEO7263015.1"
                     /translation="MNLAILALGFAVMGVSIGEGILVANIAKSAARQPEMFSKLQTLM
                     FTGVAFIEGTFFVLFAFTFLVR"
     gene            22477..23193
                     /gene="atpB"
                     /locus_tag="VBE34_000262"
     CDS             22477..23193
                     /gene="atpB"
                     /locus_tag="VBE34_000262"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEO7263016.1"
                     /translation="MESTSNPTVSFLGIDFDLTILAMSLLTITIIFILVFWASRKMTI
                     KPKGKQNVLEYVYELVNNTISQNLGHYTKNYSLLMFILFSFVFIANNLGLMTSLKTHE
                     HNFWTSPTANFGVDITLSLLVAFICHIEGIRKKGIGGYLKGFLSPTPAMLPMNLLEEV
                     TNVASLALRLFGNIFSGEVVTGLLLQLAVLSPFTGPLAFALNIVWTAFSMFIGFIQAY
                     VFIILSSSYIGHKVHGDEEE"
     gene            23211..23708
                     /gene="atpF"
                     /locus_tag="VBE34_000263"
     CDS             23211..23708
                     /gene="atpF"
                     /locus_tag="VBE34_000263"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEO7263017.1"
                     /translation="MSILINSTTIGDIIIVSGSVLLLFILIKTFAWKQITGIFEAREQ
                     KIANDIDTAEQARQQAEAFATKREEELSNAKTEANQIIDNAKETGLAKGDQIISEAKT
                     EADRLKEKAHQDIAQNKAEALADVKGEVADLTVLLAEKIMVSNLDKEAQSNLIDSYIK
                     KLGDA"
     gene            23708..24244
                     /locus_tag="VBE34_000264"
     CDS             23708..24244
                     /locus_tag="VBE34_000264"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEO7263018.1"
                     /translation="MNKKTQALIEQYSKSLVEVAIEHKIVEEIQQEVAALIDIFETSE
                     LEGVLSSLAVSHDEKQHFVKTLQTSCSTYLVNFLEVIVQNEREALLYPILKSVDQELI
                     KVNGQYPIQITTAVALSPEQKERLFDIAKTKLALPNGQLVEHIDPSIVGGFVVNANNK
                     VIDASVRNQLHQFKMKLK"
     gene            24260..25765
                     /locus_tag="VBE34_000265"
     CDS             24260..25765
                     /locus_tag="VBE34_000265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEO7263019.1"
                     /translation="MAINAQEISALIKKQIEDFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFSNGAYGMAQNLESNDVGIIILGDFSEIREGDVVKRTGKIMEVPVGEA
                     MIGRVVNPLGQPVDGLGEIETTATRPVETPAPGVMQRKSVFEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRKYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYNGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDALGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGAAQIKAMKRVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEVLKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     NDILAFEEALYDYFDAHYDNLFETIRTTKDLPEEAELDAAIQAFKDQSQFK"
     gene            25781..26662
                     /locus_tag="VBE34_000266"
     CDS             25781..26662
                     /locus_tag="VBE34_000266"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEO7263020.1"
                     /translation="MAGSLSEIKDKILSTEKTSKITSAMQMVSSAKLVKSEQAARDFQ
                     VYASKIRQITTNLLKSDLVSGSDNPMLASRPVKKTGYIVITSDKGLVGGYNSKILKAM
                     MDTITDYHTENDDYAIISIGSVGSDFFKARGMNVSFELRGLEDQPSFDQVGKIIAQAV
                     EMYKNELFDELYVCYNHHVNSLTSQVRMQQMLPIKELDAEEASEDRVITGFELEPNRE
                     VILEQLLPQYTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQA
                     AITQEITEIVAGANALE"
     gene            26736..28142
                     /gene="atpD"
                     /locus_tag="VBE34_000267"
     CDS             26736..28142
                     /gene="atpD"
                     /locus_tag="VBE34_000267"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEO7263021.1"
                     /translation="MSSGKIAQVVGPVVDVVFASGDKLPEINNALIVYKNGDKSQKVV
                     LEVALELGDGLVRTIAMESTDGLTRGLEVLDTGRAISVPVGKDTLGRVFNVLGDAIDL
                     EEPFAEDAERQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALTPEIVGDEHYEVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAETFTGQPGSYVPVEETVRGFKEILDGKHDQIPEDAF
                     RMVGGIEDVIAKAEKMNY"
     gene            28155..28568
                     /locus_tag="VBE34_000268"
     CDS             28155..28568
                     /locus_tag="VBE34_000268"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEO7263022.1"
                     /translation="MAQLTVQVVTSDGIRYDHHASLITVRTPDGEMGILPGHINLIAP
                     LIVHQMKINRSHQEGVDWVAVNGGIIEVNEDQVTIVADSAERARDIDLNRAERAKERA
                     ERALEKAQTTQNIDEMRRAEVALRRAINRISVGKK"
     gene            28633..28863
                     /locus_tag="VBE34_000269"
     CDS             28633..28863
                     /locus_tag="VBE34_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEO7263023.1"
                     /translation="MILNIVTIVSNFIFIALFYQLFVDLFDWSKMIKMSPQNISKLKV
                     FILLISIVGGYVVSHFLLEVIQLCQSIFWALQ"
     gene            28926..30197
                     /gene="murA"
                     /locus_tag="VBE34_000270"
     CDS             28926..30197
                     /gene="murA"
                     /locus_tag="VBE34_000270"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO7263024.1"
                     /translation="MDKIIVEGGQTRLQGQVVIEGAKNAVLPLLAATILPSQGKTLLT
                     NVPILSDVFTMNNVVRGLDIQVDFNCDKKEILVDASGDILDVAPYEFVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPIDLHLKGLEAMGATITQNGGDITAQAEKLKGANI
                     YMDFPSVGATQNLMMAATLASGTTTIENAAREPEIVDLAQLLNKMGAKVKGAGTETLT
                     IIGVDALHGTEHDVVQDRIEAGTFMVAAAMTSGNVLVKDAIWEHNRPLISKLMEMGVE
                     VSEEEDGIRVKADTKKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMIETVFEN
                     RFQHLEEMRRMGLQTEILRDTAMIHGGRALQGAPVMSTDLRASAALILAGMVAQGQTV
                     VGQLTHLDRGYYQFHEKLAALGANIKRVSEA"
     gene            30199..30390
                     /locus_tag="VBE34_000271"
     CDS             30199..30390
                     /locus_tag="VBE34_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO7263025.1"
                     /translation="MASSGWKYVLKQIGLIVLVILLALLFLAVGLMLGYSVFGDGEHA
                     YSILSLDKWQNIIGKFLGK"
     gene            30465..31322
                     /locus_tag="VBE34_000272"
     CDS             30465..31322
                     /locus_tag="VBE34_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO7263026.1"
                     /translation="MTKKQKQITASIVTLLFSLLAIGLGESKSLPNDHILKQVTDLII
                     SNESGKNTSLKNISGTPSRELSESVLTSNVKKQLGTNIAWNQSGAFIINQNKTDLNAK
                     VSSAPYAINEIKKVNNQIVPTKANALLTKATRQYRNREETGNGRTYWKPAGWHQINGL
                     KGSYNHAVDRGHLIGYALVGSLRGFDASTSNPKNIATQAAWANQANSNQSTGQNYYET
                     LVRKALDRHKTVRYRVTLIYDRDNLLSSGSHIEAKSSDGSLEFNVFIPNVQSGLLFDY
                     ATGKVKQTK"
     gene            31613..32653
                     /gene="pheS"
                     /locus_tag="VBE34_000273"
     CDS             31613..32653
                     /gene="pheS"
                     /locus_tag="VBE34_000273"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEO7263027.1"
                     /translation="MDLQKQLEELKISTQEKLKEMTGNHTKELQDLRVQVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDILTKAFEEQAKVVEAAKIQAQLESESVDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQVVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTMDQHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQI
                     EGLVVGENISMGDLKGTLQLISQKMFGAERKIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKQTGWIEILGAGMVHPSVLEMSGIDSEKYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDVRFTDQF"
     gene            32736..33256
                     /locus_tag="VBE34_000274"
                     /pseudo
     CDS             32736..33256
                     /locus_tag="VBE34_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            33310..35715
                     /locus_tag="VBE34_000275"
     CDS             33310..35715
                     /locus_tag="VBE34_000275"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEO7263028.1"
                     /translation="MLVSYKWLKELVDVDVTTAELAEKMSTTGIEVEGVETPAEGLSK
                     LVVGHIVSCEDVPDTHLHLCQVDTGDDELRQVVCGAPNVKTGINVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSESIIPKEFSDGIQILPEGAIPGDSIFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYGKKVHFEEKNLIEEAERAADKISVVIESDK
                     VLSYSARIVKNVTVAPSPQWLQNKLMNAGIRPINNVVDVTNYVLLTYGQPMHAFDFDK
                     FDGTTIVARNAENGEKLITLDGEERDLIADDLVIAVNDQPVALAGVMGGQSTEIDSSS
                     KTVVLEAAVFNGTSIRKTSGRLNLRSESSSRFEKGINYDTVSEAMDFAAAMLQELAGS
                     QVLSGQVTEGVLPTEPVEVSTTLGYVNTRLGTELTYTDIEEVFEKLGFAISGSEVKFT
                     VLVPRRRWDIAIQADLVEEIARIYGYEKLPTTLPEAGATAGELTSMQRLRRRVRTVAE
                     GVGLSEIITYALTTPEKAVQFSTQATNITELMWPMTVDRSALRQNVVSGMLDTIAYNV
                     ARKNSNLAVYEIGKVFEQTGNPKEDLPTEVETFTFALTGLVEEKDFQTKAKPVDFFYA
                     KGIVEALFIKLKLDVTFVAQKGLASMHPGRTATILLDGKEIGFVGQVHPQTAKQYDIP
                     ETYVAEINLSTIESQMNQALIFEDITKYPSVSRDIALLLAESVSHHDIVLAIESSGVK
                     RLTAIKLFDVYAGNNIAEGYKSMAYSLTFQNPNDNLTDEEVAKYMEKITKSLVEKVNA
                     EIR"
     gene            complement(35784..36686)
                     /locus_tag="VBE34_000276"
     CDS             complement(35784..36686)
                     /locus_tag="VBE34_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEO7263029.1"
                     /translation="MKIDDLRKSDNVEDRRSSSGGSFSSGGSGLPILQLLLMRGSWKT
                     KLVVLIILLLLGGGGLTSIFNDSSSPSSYQSQNVSRSVDNSATREQIDFVNKVLGSTE
                     DFWSQEFQTQGFGNYKEPKLVLYTNSIQTGCGIGESASEPFYCSADKKIYLDISFYNE
                     LSHKYGATGDFAMAYVIAHEVGHHIQTELGIMDKYNRMRHGLTKKEANALNVRLELQA
                     DYYAGVWAHYIRGKNLLEQGDFEEAMNAAHAVGDDTLQKETYGKLVPDSFTHGTAQQR
                     QRWFNKGFQYGDIQHGDTFSAEHL"
     gene            36864..40097
                     /gene="rexB"
                     /locus_tag="VBE34_000277"
     CDS             36864..40097
                     /gene="rexB"
                     /locus_tag="VBE34_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEO7263030.1"
                     /translation="MKLLYTDINHDMTEILVNQAAHAAEAGWRIFYIAPNSLSFEKER
                     AVLETLPQEASFAITITRFAQLARYFTLNQPNQKESLNDIGLAMIFYRALASFEDGQL
                     KVFGRLKQDASFISQLVDLYKELQTANLSILDLKYLHSPEKFEDLLAIFLVVSDLLRE
                     GEYDNQSKIAFFTEQVRSGQLDVDLKNTILIVDGFTRFSAEEEALIKSLSSRCQEIII
                     GAYASQKAYKTNFTNGNIYSAGVDFLRYLATTFQTKPEFILSKWESKSGFEMISKNIE
                     GKHDFTNSSHILDDTAKDCITIWECINQKDEVEHVARAIRQKLYQGYRYKDILVLLGD
                     VDSYKLQLSKIFEQYDIPYYFGKAETMAAHPLVHFMDSLSRIKRYRFRAEDVLNLFKT
                     GIYGEIGQDDLDYFEAYISYADIKGPKKFFTDFVVGAKKFDLGRLNTIRQSLLTPLES
                     FVKTKKQDGIKTLNQFMFFLTQVGLSDNLSRLVGQMSENEQEKHQEVWKTFTDILEQF
                     QTIFGQEKLNLDEFLSLLNSGMMQAEYRMVPATVDVVTVKSYDLVEPHSNQFVYALGM
                     TQSHFPKIAQNKSLISDIERQLINDANDTDGHFDIMTRENLKKNHFAALSLFNAAKQA
                     LVLTIPQLLNESEDQMSPYLVELRDIGVPFNHKGRQSLKEEADNIGNYKALLSRVVDL
                     YRSAIDKEMTKEEQTFWSVAVRYLRRQLTSKGIEIPIITDSLDTVTVSSDVMTRRFPE
                     DHPLKLSSSALTTFYNNQYKYFLQYVLGLEEQDSIHPDMRHHGTYLHRVFEILMKNQG
                     IESFEEKLNSAINKTNQEDVFKSLYSEDAESRYSLEILEDIARATATILRQDSQMTVE
                     SEEERFELMIDNTIKINGIIDRIDRLSDGSLGVVDYKSSAQKFDIQKFYNGLSPQLVT
                     YIDAISRDKEVEQKPPIFGAMYLHMQEPRQDLSKIKNLDDLVTKNHQALTYKGLFSEA
                     EKEFLANGKYHLKDSLYSEAEIAILQAHNQSLYKKASETIKSGKFLINPYTEDAKTVD
                     GDQFKSITGFEADRHMARARALYKLPAKEKRQGFLTLMQQEEENDDL"
     gene            40087..43710
                     /gene="addA"
                     /locus_tag="VBE34_000278"
     CDS             40087..43710
                     /gene="addA"
                     /locus_tag="VBE34_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEO7263031.1"
                     /translation="MTFKPFLNPEDIAVIQTEEKNSDKKQKRTPEQIEAIYTFGNNVL
                     VSASAGSGKTFVMVERILDKLLRGVPIESLFISTFTVKAAGELKERLEKKINESLKSA
                     ESDDLKQFLTQQLVGIQTADIGTMDAFTQKIVNQYGYTLGISPIFRILQDKNEQDVIK
                     NEVYADLFSDYMTGKNAASFIKLVKNFSGNRKDSKAFREMVYKVYAFSQSIDNPKRWM
                     QTVFLKGAQTYTDFEAIPDQEVSSLLNVMQTTANQLRDLTDQEDYKQLTAKGVPTANY
                     KKHLKIIENLVHWSQDFNLLYGKKGLANLARDITNVIPSGNDVTVAGVKYPIFKQLHN
                     RIVGLKHLEVIFKYQGESLFLLELLQSFVLDFSEQYLQEKIQENAFEFSDIAHFAIQI
                     LEENHDIRQIYQDKYHEVMVDEYQDNNHTQERILELLSNGHNRFMVGDIKQSIYRFRQ
                     ADPQIFNDKYKAYQDNPSQGKLIILKENFRSQSEVLDSTNSVFTHLMDEEVGDILYDE
                     SHQLKAGSPRQQERHPNNKTQVLLLDTDEDDIDDSDSQQYDISPAEAKLVAKEIIRLH
                     KEENVPFQDITLLVSSRTRNDGILQTFDRYGIPLVTDGGEQNYLKSVEVMVMLDTLRS
                     IDNPLNDYALVALLRSPMFGFNEDDLTRIAIHDVKMAFYHKVKLSYHKEGHHSDLITP
                     ELSSKIDYFMKTFQTWRDFAKWHSLYDLIWKIYNDRFYYDYVGALPKAEQRQANLYAL
                     ALRANQFEKTGFKGLSRFIRMIDKVLENENDLADVEVALPQNAVNLMTIHKSKGLEFK
                     YVFILNIDKKFSMVDITSPLILSRNQGIGIKYVADMRHELEEEILPAVKVSMETLPYQ
                     LNKRELRLATLSEQMRLLYVAMTRAEKKLYLVGKASQTKWADHYDLVSENNYLPLASR
                     ETFVTFQDWLLAVHETYKKQELFYDINFVSLEELTDHHIGMVNPSLPFNPDNKAENRQ
                     SEDIVHAISVLESVEQINQTYKAAIELPTVRTPSQVKKFYEPILDIEGVDVMETITKT
                     SVDFKLPDFSTSKKQDPAALGSAVHELMQRIEMSSHVKMEDIQKALTEVNAETSIKAA
                     IQIEKINYFFQENSLGKYIQEEVEHLHREAPFAMLKEDPESGEKFVVRGIIDGYLLLE
                     NRIILFDYKTDKFVNPLELKERYQGQMALYAEALKKSYEIERIDKYLILLGGKQLEVV
                     KMD"
     gene            43723..44640
                     /locus_tag="VBE34_000279"
     CDS             43723..44640
                     /locus_tag="VBE34_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO7263032.1"
                     /translation="MIVEQKFGNGFTWINIEAEQLRTETSEIQAKYLDSEIITYALDD
                     YERAFMECSHIKGKEVLTIIFNTIDLKQKESYYETVPMTFCLSHDRLITVTRSRNSYM
                     LELLQKYLDRNPDVSPKKFLFAALTLITKQYFNVVSKIDREKDILNRQLREQTTNKRL
                     LAMSDLETGSVYLLTAANQNALVLEQLDVHPSQRFNSEVEKEQLSDALIEAHQLVSMT
                     QLNSQVLSQLSSTFNNVLNNNLNENLTGLNIISINLAIIAAITGFFGMNIPLPLTESR
                     SSWLIVIATSVLLWVIIAQILKRVFNLRK"
     gene            complement(44672..46048)
                     /gene="mnmE"
                     /locus_tag="VBE34_000280"
     CDS             complement(44672..46048)
                     /gene="mnmE"
                     /locus_tag="VBE34_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEO7263033.1"
                     /translation="MSITKEFDTIAAISTPLGEGAIGIVRISGTDALKIASKIYRGKD
                     LSAIQSHTLNYGHIVDPDKNEILDEVMLGVMLAPKTFTREDVIEINTHGGIAVTNEIL
                     QLILRHGARMAEPGEFTKRAFLNGRVDLTQAEAVMDLIRAKTDKAMDIAVKQLDGSLK
                     TLINNTRQEILNTLAQVEVNIDYPEYDDVEEMTTTLMREKTQEFQALMENLLRTARHG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREEKAIVTDIEGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRDTDDIVEKIGVERSKKALEEADLVLLVLNSSEPLTLQDRSLLELSKESNRI
                     VLLNKTDLPQKIEVNELPENVIPISVLENENIDKIEERINDIFFDNAGMVEHDATYLS
                     NARHISLIEKAVDSLKAVNEGLELGMPVDLLQVDMTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     gene            46176..48086
                     /locus_tag="VBE34_000281"
     CDS             46176..48086
                     /locus_tag="VBE34_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO7263034.1"
                     /translation="MIILQGNKIERSFSGDVLFDNINIQVDQRDRIALVGRNGAGKST
                     LLKILVGEEAPTKGEINKKRDLSLSYLAQDSRFQSENTIFQEMLQVFDSLREVEKRLR
                     ELELQMGQVSGSDLEQLMKTYDILSEEFREKGGFTYESDIKAILNGFKFNSDMWEMPI
                     SELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIDTIAWLENYLVNYQGALIIVSHD
                     RYFLDKVATVTYDLTTHSLDRYVGNYSKFMDLKAEKIATEEKNFEKQQKEIAKLEDFV
                     QRNIVRASTTKRAQARRKQLEKMERLDKPNVEQKSANMTFHAGKVSGNVVLTLENAAI
                     GYEGVSLSEPIDLDVKKFDAIAIVGPNGIGKSTLIKSLVGQIPFIKGEAKLGANVETG
                     YYDQSQSNLTKTNTVLDELWNAFSTTPEVEIRNRLGAFLFSGDDVKKSVSMLSGGERA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIEFDGTLLFVSHDRYFINRVAT
                     KVLEISDKGSTLYLGDYDYYLTKKAELEELARLNEEEVSASKTEIDVTSDYETQKANQ
                     KEFRKITRRVAEIEARLEVLENDENNINGLMLETNDIGKLSDLQKELESIQEEQLLLM
                     EEWENLNMRLDL"
     gene            48236..49204
                     /locus_tag="VBE34_000282"
     CDS             48236..49204
                     /locus_tag="VBE34_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEO7263035.1"
                     /translation="MVTLSKEQHLDMFLKMQRIRDVDMKFNKLVRRGFVQGMTHFSVG
                     EEAASVGAIQDLTDSDIIFSNHRGHGQTIAKGIDIGGMFAELAGKATGTSKGRGGSMH
                     LANLEKGNYGTNGIVGGGYALAVGAALTQQYEGTDNIVIAFSGDSATNEGSFHESVNL
                     AAVWNLPVIFFIINNRYGISTDITYSTKIPHLYMRADAYGIPGHYVEDGNDLMAVYEK
                     MHEVINYVRSGNGPAIVEVESYRWFGHSTADAGVYRTKEEVDSWKAKDPVKRYRAYLI
                     ENEIATEEELAAIEAQVIKEVEEGVKFAEESPFPDMSVAFEDVFVD"
     gene            49279..50277
                     /locus_tag="VBE34_000283"
     CDS             49279..50277
                     /locus_tag="VBE34_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEO7263036.1"
                     /translation="MSETKVMALREAINVAMSEEMRKDEKVFLMGEDVGVYGGDFGTS
                     VGMLEEFGAKRVRDTPISEAAIAGSAIGAAQTGLRPIVDLTFMDFVTIAMDAIVNQGA
                     KTNYMFGGGLSTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGLKVVAPGTVNESKAL
                     LKSSILDNNPVIFLEPKALYGKKEEVNMDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVMQAAEEVAEEGINVEVVDPRTLIPLDKELIIDSVKKTGKLILVNDAYKTGGFTGEI
                     ATMVAESEAFDYLDHPIVRLASEDVPVPYSRVLEQGILPDVAKIKDAIYKVVNKGK"
     gene            50404..51792
                     /locus_tag="VBE34_000284"
     CDS             50404..51792
                     /locus_tag="VBE34_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEO7263037.1"
                     /translation="MAVEIIMPKLGVDMQEGEILEWKKQVGDVVNEGDVLLEIMSDKT
                     NMEIEAEDSGVLLKITHGNGDVVPVTETIGCIGAEGEEVTEASSSENTSVEENATQVT
                     SEPEKVEETSEPSVPAATSGEKVRATPAARKLAREMSIDLALVSGTGANGRVHREDVE
                     SFKGAQPRITPLARRIAEDQGVDIAEITGSGIRGKIVKNDVLAAMSPQAAEAPVKTKA
                     TPTTEEKQLPEGVEVIKMSAMRKAISKGMTNSYLTAPSFTLNYDIDMTEMMALRKKLI
                     DPIMAKTGLKVSFTDLIGMAVVKTLMKPEHRYLNASLINDAQEIELHNFVNIGIAVGL
                     DDGLIVPVVHNADQMSLSDFVIASKDVIKKTQEGKLKSAEMSGSTFSITNLGMFGTKT
                     FNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRIVDGMNGAKFMVDL
                     KNLMENPFGLLI"
     gene            51852..53609
                     /gene="lpdA"
                     /locus_tag="VBE34_000285"
     CDS             51852..53609
                     /gene="lpdA"
                     /locus_tag="VBE34_000285"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEO7263038.1"
                     /translation="MAFDVIMPKLGVDMQEGEILEWKKNEGDTVNEGDVLLEIMSDKT
                     NMEIEAEDTGVLLKIVHQAGDVVPVTEVIAYIGEEGEEVGTSSPSADATITAEDGQSV
                     SGPAAPSQETVAAATPKEELAADEYDIVVVGGGPAGYYAAIRGAQLGGKIAIVEKTEF
                     GGTCLNVGCIPTKTYLKNAEILDGLKVAAGRGINLASTNYAIDMDKTVAFKNSVVKTL
                     TGGVRGLLKANKVEIFNGLGQVNPDKSVVIGDKVIKGRNVVLATGSKVSRINIPGIES
                     PLVLTSDDILDLREIPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAMDKE
                     VSLELQKILAKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVADKALLSIGRVPQMN
                     GLENLEPELEMERGRIKVNAYQETSIPGIYAPGDVNGTRMLAHAAYRMGEVAAENALG
                     GNKRKAHLDFTPAAVYTHPEVAMVGMTEEQAREQYGDILVGKNSFTGNGRAIASNEAH
                     GFVKVIAEPKYKEILGVHIIGPAAAELINEASTIMENELTVYDVAQSIHGHPTFSEVM
                     YEAFLDVLGEAIHNPPKRK"
     gene            53707..54696
                     /locus_tag="VBE34_000286"
     CDS             53707..54696
                     /locus_tag="VBE34_000286"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO7263039.1"
                     /translation="MKYIVNTSNDPAYNVALEAYAFQKLTDIDEIFILWINEPAIIIG
                     RHQNTIQEINKEFIDKNGIHVVRRLSGGGAVYHDLNNLNYTIISNNTQEGAFDFQTFS
                     KPVIDTLAKLGVKAEFTGRNDLEINGQKFAGNAQAYYKGRMMHHGCLLFDVDMSVLGQ
                     ALKVSKDKIESKGIKSVRARVTNIVDHLSDKITVQEFSDAILAQVKEEYPEMDEYVLS
                     DAELSEIQAMRDNQFATWDWTYGKAPEYTIERGVRYPAGKITTYANVENSTIKSVKIF
                     GDFFGVKPVDDIEKMLEGVRYDYKDVLAALKTVDTSQYFSRMTPEEITKAIVD"
BASE COUNT        17871 a         8557 c        11137 g        17191 t
ORIGIN      
        1 tatcaagata taatggatca ctatttttgg ttggaaaaat ggttgataag ggtcgatttt
       61 gattatatgc tcaatcgttt gaatgtcttc gaattagaaa agaaaagtca taatacaggt
      121 gacaaactgt tatcaattaa agatttccaa gttaaacttt caaaaaacaa attcatctct
      181 taccttgatt ttgatttagc aagtggggag aggttgtgct tagatggacc aagtggcgtg
      241 gggaagtcta gtttctttat gggattgtta gggctctata ggactaaagg aaagaaacaa
      301 tttactcata gaaagcaaat tcctataagc tttttatttc aaaatccctt agatcaattt
      361 attttttcaa cagtttatga cgaaattttt caggtttgca aggatagtaa caaagcgagg
      421 gatattttgg aaaccataaa tctttgggat aaaaaacagt tttcaccatt tcaactaagt
      481 caaggccaac agaggcgatt agccattggc agtattttag ctagtgatag taaacttcta
      541 ttattagatg aaccaactta tggacaagat gcctatcacg ctaatatgat tacgactctt
      601 cttttgagtt attgtcacaa aaatcattgt ggggttattt ttactagcca tgatcctcac
      661 ttaaaagagt gttttgcaac acgtttcttg gaggtgtcat cttgttcaat ttgaaaaact
      721 atcatccagc tatcaccttc ctatgtttat taataccaac cttttcgttt agtttcacat
      781 tgaggctaag aacgtctttg ttatttctaa ttatagtagt aacactgcaa tgcttcgtaa
      841 aggtttcttt aaaaacttgg gcgaaggtca atttgatctc atttgtgatg ggactctctt
      901 tatttttagg gacgtatttt tgggggaaat taccccatca attcgtctta gctagtctag
      961 tggcctgtcg tccgcttatt tttatgaacg tgggtttgtt atttcatgca tcacattcta
     1021 attatgattt tatagaatct ctttatcaaa ctttcaaagt gcctagtcat tttgcatatg
     1081 gtatttttgc ggtctttaat cttttaccat taattaaatt acaataccaa cgtaatcgac
     1141 tcgcattccg actgaagaat caggtaactt gggcattatc gcctagacta atattgtcag
     1201 ttttattaaa gacgatatat tgggtagaac agttagagtt agcaatgctt tctaaaggtt
     1261 ttgaagtggg taaagagcga acacatgctt caacttatcc cgttcgtttt cgagattata
     1321 gtttacttgg tatgagtatt ttattggcta taggaatgat ttttaaataa gtacaattag
     1381 tttagtaata caattgtgca tttatataga gtgagcatgt tctgctcatg agatcataac
     1441 aaaggagatt actatgttaa aagatgagat gattgctaaa ggaacaaaat atatacaatc
     1501 catctatcaa gatccattta ttcaaggaat tgtaaaaggg cgtttagacc atgacgtaat
     1561 ctgccattat ttacaggcag ataatatcta ccttgggaaa tttgctgata tttatgccct
     1621 gtgcttggca aaatctgata atttacgaga taaacaattt ttccttgaac aagttgactt
     1681 tacattaaat cgggagttag cagatggtga aggtccacat caagccttgg cagcttatac
     1741 taatcgatct tatcaggata ttatcgaaaa ggctgtatgg tacccgtcag cagatcatta
     1801 cattaaacat atgtattttc atttttacga aaatggtatt gctggtgctt tagctgcaat
     1861 gagcccatgt ccatggattt accatcaatt ggctaaaaaa ataattgagg agaaccagtt
     1921 tttgaatggt aaccctttta ataattggat aactttttat gctaacgata cagttgaaga
     1981 gttaatggaa aactattttc gtatgatgga ttattatgcg cagaatttgt caaaagagaa
     2041 gcaagctgat ttggtagatg cttttgtgaa atcttgtcag catgagagac gtttttttca
     2101 aatggcaata aatcaagaaa aatgggagga ttaatatgac ttatttagca ccagttttaa
     2161 ctattgcagg aactgatcct agcggtggag cagggattat ggcagattta aagacatttc
     2221 aagctcgtcg gacttacggg atggctgttg tgactagtgt tgttgctcag aacacttgtg
     2281 gggttagagg cgttcagcat atagaaacag ctatcattga tcaacaacta gcttgtgttt
     2341 atgatgatat taagccaaag gcggtcaaaa caggaatgtt agcggagagg gaaacgatta
     2401 gtttagtcgc ttcatattta aaaaaatatc cgcaacctta tgtattagat cctgttatgg
     2461 tggcaactag cggacatcgt ttaattgatt cagatgccgt agaagcatta aaagaagact
     2521 tattgccatt agcaactatt atcacaccaa atttaccaga agctgaggtt ttagtgggat
     2581 atgacttatc agatgaagta agtattatta aagcaggtta cgatattcaa aaacaatatt
     2641 ctgttagaaa tgttcttata aagggaggac atctagatgg tttggctaaa gattatttat
     2701 ttttggaaaa agaagggttg ataactctta gtaatcaacg tatcaatact attcatactc
     2761 atgggactgg gtgtacattt gcagcagtcg ttgcagcgga gttagctaaa gggcaatcaa
     2821 ttctaaacgc ggtaagtacc gctaaatcat ttattacttc agctatcgaa acagcgcctg
     2881 agttgggtct tggaaacggt cctgtcaatc atactagcta tcaaggagac taaaatgtat
     2941 ttagaacaac taaaagaggt aaatccttta accatttgta tcactaataa tgtggtcaaa
     3001 aactttacag ccaatggttt attagcttta ggagcttctc cggctatgag cgagtgcata
     3061 gaggacttag aagacttgtt aaaagttgca gatgcattat taattaatat aggaacacta
     3121 acaaaagaaa gttggcaact ttaccaagaa gcaatcaaaa tagcaaataa aaaccaagtt
     3181 ccagttgtat tagatcctgt agcggcagga gcaagtcgtt ttcgattaga agtgtcactt
     3241 gatttattga agaattatcc tattagttta cttagaggaa atggtagtga aatcgctgct
     3301 ttggttggtg aaaaacaagc gtctaaaggg gcagatgggg gaaaagtggc tgatttagaa
     3361 tcaattgctg taaaagccaa tcaagttttt gatgtgccag tggttgtcac aggagaaact
     3421 gatgctatcg ctgttagagg agaagttcgc ttattacaaa atggtagtcc tttaatgcca
     3481 cttgttacag gaactggatg tctattaggg gcagtattgg ctgcttttat tggtagttct
     3541 gacagaagcg atgatcttgc ttgtctgaca gaggccatga ctgtttataa tgttgctgga
     3601 gaaattgcag agaaagtggc aaaaggtaag ggagtgggaa gtttccaagt tgcattttta
     3661 gatgcccttt ctcagatgaa atcagaaatg attatggaca aataggcggt gtcagtagat
     3721 gaaagataca ttgaaattat attttgtttg tgggactgtg gattgctcta ggaagaatat
     3781 attgactgtt gttgaagaag ccttacaagc aggtataact ctatttcaat ttagagaaaa
     3841 aggttttaca gcattacaag gtaaggaaaa aattgctatg gctaagcaac tccaaatact
     3901 ttgtaagcaa tatcaagttc cttttattat tgatgatgac attgacttag ttgagttaat
     3961 tgatgctgat ggtcttcata ttggacaaaa tgatttacct gttgatgagg cacggagacg
     4021 tttaccagat aaaatcatcg gtttatctgt gagtacaatg gctgaatatc agaaatcaca
     4081 attatctgtt gttgattaca tcggaattgg tccttttaat ccgacacaat caaaagcaga
     4141 tgctaagcct gctgttggca ataggacaac aaaggcagtt agagaaatta atcaagatat
     4201 tccaatcgtt gcaataggcg gaatcactag tgattttgtt catgatatta tagaatcagg
     4261 cgcagatggg attgctgtta tatcggctat ttcaaaagca aatcatattg tagatgcaac
     4321 cagacaattg cgctatgaag ttgaaaaagc tctggttaat aggcaaaaac acagtgatgt
     4381 aatcaaataa ggctacatcg ttaattataa gaatttttaa gatattatat catcacacaa
     4441 tactggtatt tgaaaaagaa tttaaactat gataatatag tagagttgaa ttctagttaa
     4501 agaaagattt gaaagtatgc gtaaaattat tattaacggt ggcaagcaac tgacgggaga
     4561 agttgctgta tcgggtgcca aaaatagtgt agttgccttg attcctgcca ctattcttgc
     4621 tgatgatgtt gtggtcttag atggagttcc ggctatttct gatgttgaca gtttagttga
     4681 cattatggaa actatgggag ctaagattaa gcgttatggt gagacgcttg aaattgatcc
     4741 acgtggcgtt aaagatattc caatgcctta tggcaaaatc aatagtcttc gtgcatccta
     4801 ttatttctat ggaagcttat tggggcgtta tggtcaagca acattaggtt tacctggtgg
     4861 ttgtgattta ggcccccgtc cgattgattt acatttaaaa gcttttgaag cgatgggagc
     4921 tagtgtttct tatgaaggtg attcaatgcg tctagcaaca aacggaaagc ctttgcaagg
     4981 agcaaatatt tatatggata ctgtcagtgt tggtgcgaca attaacacta tgatcgcagc
     5041 agctaaggca aatggtcgta cggttattga aaatgctgcg cgtgaaccag aaattattga
     5101 cgtggctact cttttaaata atatgggagc tcatattcgt ggtgctggga ctgatgttat
     5161 tacaattgaa ggtgtgaaat cgctccatgg cacgcgtcat caagtaattc cagatcgtat
     5221 tgaagcagga acttatattg ctatggcagc agctatcgga cgaggaataa aagtgaccaa
     5281 tgtgttatat gaacaccttg agagtttcat tgctaagtta gatgagatgg gagttcgaat
     5341 gacagttgaa gaggattcaa tttttgtcga agaacaagaa aagttaaaag ctgtatccat
     5401 aaaaacctca ccttacccag gatttgctac agatttacaa caacctctaa cccctttatt
     5461 actaacagca gaaggaaacg ggagtttatt agatactatc tatgagaaac gtgttaatca
     5521 tgttcccgaa ttagcacgta tgggagctaa tatttctacc ctagggggca aaatagttta
     5581 tagtggtcct aatcaattat ctggtgcacc tgtaaaagcg acagatttgc gtgcaggcgc
     5641 tgctttagtt atcgctggct taatggcaga gggacgaact gaaattacga atatcgagtt
     5701 tattttacgt ggatattcta atatcatcga gaaactaaca tcattgggtg cggatatcca
     5761 attggttgag gaatagtata tataacaaag agttgagaat atttcttagc tcttttcttc
     5821 ttgttttaga ttaagtcact tataggtatt tgaaatctct tgtggtataa tattatcatg
     5881 gatatatgga caaatttagg acggtttgca ttcattgaaa cagaacatgt taacctacgt
     5941 cctgttgcct acactgatag ggaagctttt tggcgaatag cttcaaaaag gacaaattta
     6001 caatttattt ttcctgttca aaccagtaag aaggaaagtg attttctttt agttcattct
     6061 tttatgaaag agccgttggg agtttgggcg attgaagata aagttagtca taaaatgttt
     6121 ggagttatcc gttttgaaaa cattgattta tctaaaaaga cagctgaaat tggctatttt
     6181 ttgaaagaaa gttcttgggg tcaaggcatt atgactgaat gtctaaagac actttctttt
     6241 tttgcttttc aagagtttgg gatggacaaa ttgatcatag taacacataa agagaatatt
     6301 gctagtcaga aggttgcttt gaaggcgcat ttcaaacaat ctaggagttt taaaggaagc
     6361 gaccgttata cacgccgtat aagagactat attgagtttc aattaactag gggtgattat
     6421 cgttatgagt aaacatcaag aaattttgga gtacctagaa aatttagctg ttggtaagag
     6481 ggttagtgta cgcagtattt caaatcattt aaaggtgagt gatggcactg cataccgcgc
     6541 tataaaagag gcagaaaata ggggtattgt tgagactaga ccgcgtagtg ggacagtacg
     6601 tgttgctcaa aaagcaaagg ttaatattga aaaactgact tatgctgaaa ttgcaaggat
     6661 tagtgactca caagttgttg caggaacaga ggggctcagc aaggagttta gtaaattttc
     6721 tataggtgca atgactcaca gaaatattga aaaatatttg gtgcaaggag ggttattgat
     6781 tgttggcgac cgtgatgaaa tacaacattt agccttacaa catcaaaacg ctatcttagt
     6841 tacaggtggc tttaatgttt ccccatctgt ttgtcgttta gcagataaac ttcaaattcc
     6901 cgtaatggta acacactatg atacattcac agtatcaacg atgattaatc acactttgtc
     6961 aaatgccaaa attcgtactg atttaaagac cgttgaacaa gtctatcagt cgcaaatgga
     7021 ttacggtttc ttagcgcagg atgataccgt aaaagaattt aatcttcttg ttaaacaaac
     7081 taaaaatgtt cgttttccaa tagtcaacca agctaatgtt gtcgttggtg ttgttagtgt
     7141 gcaagatatt cttggaaaag ataaagaagt aaaattggca acagtcatgt caaaaaatat
     7201 cattgttgct aaacctcgaa tgagtctagc taatatcagt caaaaaatga tttttgaaga
     7261 tttgaatatg atgccagtag tatcggatga ctttgaatta ttaggcgtta ttacaagacg
     7321 acaagctgta gaaaatttat cgatgtcgca agggacagct ttatacacct atagtgatca
     7381 aatcttgtct aatttacaaa ttgaagatgg tcacttttcg tttttagtag aacccgctat
     7441 gattgaccat acaggtagct taacacaagg agttttgacg gagttcttaa aagaaatttg
     7501 tattcgtgtc ttaactcgta agcatcaacg cagtattgtt gttaaacaaa tgacacttta
     7561 tttcttgcaa cctgttcaaa ttgatgaaat aataatggta acgccaacaa ttatttcaga
     7621 aaaacggcgc gaagctacac tcgatttaga attaaaatta gaaaataaaa ttattgctaa
     7681 agctatgata gcggtaaaaa ttaattagat ataaggagaa taaatagaaa atgattacat
     7741 taaagtcagc acgcgaaatt gaagcaatgg atagagcagg tgatttttta gccagcatcc
     7801 acattggact tcgtgatatt atcaaaccag gagttgatat gtgggaagtt gaagaatacg
     7861 ttcgtagacg ttgtaaagaa gaaaacgtat tgccattgca aatcggggtt gatggtgcag
     7921 ttatggatta cccatatgct acttgttgtg ggcttaatga tgaagtggct catgcctttc
     7981 ctcgtcatta taccctaaaa caaggtgacc ttttaaaagt agatatggtt ttaagcgaac
     8041 ctttagataa atcaattgtt gatgtttcat cgttaaactt tgacaacgtg gctcaaatga
     8101 aaaagtacac ggaaacatat agtggtggtt tggcagactc atgttgggct tatgctgtag
     8161 gtgaagtttc acaagaggtg aaagacttaa tgtctgtgac aagagaagca atgtatattg
     8221 ggattgaaaa agcagttatc ggaaaccgta ttggtgatat tggagctgct attcaggatt
     8281 atgcagagag tcgtggttat ggagttgttc gtgatttggt tggtcacggt gttggtccaa
     8341 caatgcatga agagcctatg gtgcctaact atggtacagc aggtcgaggt ctccgtcttc
     8401 gtgaggggat ggttttaacc atcgaaccaa tgattaatac tggtacttgg gaaattgata
     8461 cagacatgaa aactggttgg gcacataaaa ccttagatgg tggtctttct tgtcaatatg
     8521 aacatcaatt tgttatcact aaagatggtc ctgttatttt aacgagtcaa ggagaagaaa
     8581 gaacttacta aaatgaaatt aaaaaaattc tttgaggatt tgctggctaa actagaatat
     8641 agacccattc aagtttttat gcgtcatttt caaagtgcag agatggactt atcagccata
     8701 gcagttgctt attatctatt agtgacagct tttccattac tagttattgc tgctaatatt
     8761 tttccttatt ttcatattaa tgtgtcggat ttattgtcac ttatgcaaaa aaatcttccg
     8821 aaaaatattt atgaacctgc ctcacgctta gcagtagatg ctttttcgaa accgtctacc
     8881 ggcattttgg gattcgcgtc attaacagct ttttggacaa tgtctaaaag tttgacctct
     8941 cttcaaaaag ccattaataa agcatacggt gttgaccaac acagagactt tgtgattagt
     9001 cgtttagtgg gtgtggggac tgggctgatc atcctatttt tactgacttt tgttttaatt
     9061 ttttcaacct tttcaaaacc agttttacag ataattgtaa acatgtatga tttgggagat
     9121 actctaacgg cgtggctttt aaatcttgca caaccagtta catttttgac tatattttta
     9181 ggtattggaa ttttatactt tattttacct aacgcacgaa ttagaaaagt acgttatgtc
     9241 attccaggga cattattttc aacgtttgtt attggttttt ttagtaattt aattagccaa
     9301 tacgtcttaa atagggttga aaaaatggta gatattaaaa cgtttggttc ggtcgttatc
     9361 tttatcttaa tgctatggtt tattttctta gcccacatta tgattttagg tgcaatactg
     9421 aatgccagcg ttcaagaaat tgcgacagga aaaattgaat caaggcgtgg agatattatg
     9481 tctcttatcc aaaaatccaa agaagaaaaa taagaaaaga cagctcttgt tagagttgcc
     9541 tttttttgtc tttaaaaata acaaatttgc tcaagatgta gttgagtaaa ataattaata
     9601 tttgagatac aaggctttca atggtgttga ttttatttaa attgtgctga acgaattgtc
     9661 cgataataga aggaaattga tcaacaaaaa taacagctaa tatcaaatct aatcctaatg
     9721 tgcctaatct agcgatgaca aatgtttgta agcgttgaaa ctgtccaatt ttagtttgtt
     9781 taaaaacaaa tcgatcattt gtaaaaaaag caaaaagtat agctattatg ttagcaacta
     9841 ttgcagagaa tggagcgtcc tgcgagaaat taaaaatccc ttgtcgaata actaaataaa
     9901 gaagggatgt taatagtccg aacagtaaat ataagctgac ttcattttga atactttttt
     9961 tcagtaacga ttttaattta accatatttt tattatatca cggatgcttc aaagtgatga
    10021 ggaaatctcc cataagcttg gctttacttt ttgtttgtga tatacttagt aagttgtttt
    10081 acacaaccct tgatgcttag ttccctttca tcaagcatat tacaaacgtt agaagtgtta
    10141 actgtctaaa cgttaaagga gaaattatga acacatttac aactcgcgat tacgctcaca
    10201 tggctattgt aacagctatc tatatcgttt taaccataac gccacctttt aatgctattg
    10261 cttatggtgc ttatcaattc cgtgtttcag aaatgctaaa tttcttggcc ttttatcacc
    10321 gaaaatacct ttttgcggta acgttaggat gtatgatttc taatctttat agttttggaa
    10381 tgattgatgt ctttgtaggt ggtggttcaa ctttactttt tgtttatctt ggaaccattt
    10441 tattcaagca atatcaaaaa gattatctat ttaatggcct tattaacaaa gcattctttt
    10501 tcttttcttt cttctttgct gcttcaatga ttacggtagc agttgaattg aaaattgtag
    10561 caggattacc tttattatta acatggttaa cgacagctgt tggtgaattg acttcacttt
    10621 tggttggagc agtccttgtg gataagttga gtagacacgt cgattttaca aaataaatat
    10681 attataaagc gcgagacatt gatctcggct tttttgtttg gttttatagt tagcatagct
    10741 agaaaatgct gcaactttta tgagaaatgc tccaattttt attaatatcc ttagtctttg
    10801 tgataaaata gaaatatgga aaatcgtatg aatgaattag tatctttgtt gaatcagtac
    10861 gcaaaagagt attatacaca agataatccc acagtttctg acagtcaata tgaccagtta
    10921 tatcgagagt tagtagaatt agaagagcaa catccagaaa acattttgcc aaatagtccc
    10981 acacatcgag tgggtggttt agttttagaa ggctttgaaa aataccaaca tgaatatcct
    11041 ttgtattcat tgcaggatgc tttctcaaaa gaagaattga tagcctttga taaacgtgtt
    11101 aaagcagagt ttccaactgc ttcttatatg gcagagctga aaattgatgg tctatctgtc
    11161 tcgttgactt atgtaaatgg tattttacag gttggggcaa cacgtggtga tggcaatatt
    11221 ggagaaaata ttactgaaaa tttaaagcgt gttcatgata ttcctctaca tttagaccaa
    11281 agtttagata ttactgttcg cggagagtgc taccttccca aagaatcttt tgaagctatt
    11341 aacatagaaa aacgtgcaaa tggtgagcag gaatttgcta atcctaggaa tgcagcagca
    11401 ggaaccttgc gccaattaaa cacagggatt gttgcgaaac gtaaattagc aacttttctt
    11461 tatcaagaag ctagccctac gcaaaaagaa acacaagatg atgttttaaa agagctagaa
    11521 agctatggct tttcagtcaa tcatcatcgt ttaattagct cttctatgga gaaaatttgg
    11581 gattttatcc aaactataga aaaagataga atttccctcc cttatgatat cgatggtatt
    11641 gttattaagg ttaatagctt agcaatgcaa gaagagttgg gcttcactgt aaaggcacct
    11701 cgttgggcga ttgcatataa attccctgct gaagaaaagg aagcagaaat attatctgtt
    11761 gattggacag ttgggcgaac aggagttgta acaccaacag ctaacctaac tccagttcaa
    11821 ctagcaggga caaccgttag tagggccact ctgcataatg tagattatat tgccgaaaaa
    11881 gatatccgta ttggagatac tgttgttgta tacaaagcag gggatatcat ccctgcagtc
    11941 ttaaatgtcg ttatgagtaa gcgaaaccag caagaagtca tgcttattcc taaactatgt
    12001 ccttcttgtg gtagtgaatt agttcatttt gaagatgagg tggctttacg ttgtattaat
    12061 ccgctgtgcc ccaaccaaat caaagaacgt ttagctcatt ttgctagccg ggatgctatg
    12121 aatattacag gttttggtcc atccttagtc gaaaaattat ttgatgctca tttaatagca
    12181 gatgttgcag atatttatcg tttaagtata gaggatttat taactttaga tggtattaaa
    12241 gagaaatctg caactaagat ttatcatgca atccaatctt caaaagaaaa ttctgctgaa
    12301 aaacttcttt ttggcctagg tattcgtcat gtgggaagca aggcgagccg ccttttattg
    12361 gaggaatttg gtaatttgag gcaactaagt caagcaagtc aagaaagtat tgcctcaatt
    12421 gatggactag gaggggttat tgccaaatca cttcatacgt tctttgaaaa agaagaggta
    12481 gataaactac tagaggaatt aacttcctat aacgttaatt ttaattatct aggaaaacga
    12541 gttagtacag atgcacaact ttcaggttta acagtcgttt taaccggaaa acttgaaaag
    12601 atgactcgta atgaagcaaa agagaaatta caaaaccttg gtgctaaggt gacaggtagc
    12661 gtttctaaaa agactgattt aattgttgca ggaagcgatg caggaagtaa actaaccaag
    12721 gctcaagatt tagggattac aatacaagat gaagactggt tactcaacct ttaagtgagg
    12781 aagaaatgaa gaaacaaaaa aaagcgcgtt taatttataa cccaacttct ggtcaagaaa
    12841 ttatgaaaaa aaatgtagcg gaagttcttg atatacttga aggatttggg tatgaaacat
    12901 cagcctttca aacaacgcca actaaaaatt ctgccagaga tgaagctgct agagctgctc
    12961 aagctggttt tgatttgatt gttgcagcag gtggagatgg tactattaat gaagttgtta
    13021 atggtattgc tcctttgaaa cgtcgtccta agatggcaat tattccaact ggaacaacta
    13081 atgattttgc tcgtgcctta aaaataccgc gaggtaatcc tattgaagca actaaattaa
    13141 ttggtaaaaa tcaaatcgtt aagatggaca ttggtcaagc tcaagaggac aattatttta
    13201 ttaatattgc agctgcaggt agtttgacag agttaactta tagtgtacca agtcaattga
    13261 aaacaacgtt tggctatctt gcttatctag caaaaggtgt tgaattatta cctcgagttc
    13321 gaaaagtacc agtcaaaatt actcacgata agggtgaatt tatcggtgat gcttcaatga
    13381 tttttgttgc catcacaaat tctgtaggag ggtttgaaca aattgcacct gatgccaaat
    13441 tggatgatgg gaaatttacc cttattttag taaaaactgc aaatcttatt gagattatgc
    13501 atctcattcg tttggtgcta gcaggtggaa aacatattaa tgataaacga gtagagtata
    13561 ttaaaacgtc ttatctaact atcgaacccc tatcagacga gcgtatgatg attaacttag
    13621 atggagaata tggaggagat gctcccatta ctctagcaaa ccttaaaaat catattaggt
    13681 tctttgctaa tactgatgaa atttcggatg atgctttagt tttagataaa gatgaattag
    13741 cgatagaagc tattgcacag aagtttgcta atgaggtaaa tgatttagaa ggattaggag
    13801 attgatgaat ggataatact gtcattgtac attaccatag ccgaaagaaa aattacttta
    13861 atttaagttt atggcagtgg cgcgatggct cagagggtca agatgctcat ttttcacggt
    13921 ttgacagttt cggtgctgtt gcgatactaa aatatccctt gccatatttt ttaagccatt
    13981 attatgtgat tgttaaggat aaaaattggc atcataaaac aatagattat cgcattgatt
    14041 gtactaaagg tggtgcacgt aaagaagttt ggattgtaga tggggatgac aatctgtatt
    14101 attctcgtca agcagcagtt agtagccatc attatagtag acgccaacct catgcctacg
    14161 atatggcaat caatagtaga gcgtttgata aaaaatgggg atttgatggt tggttaggtt
    14221 ttgaatattc agaaactaaa acggctttta gattgtgggc ccctactgcg gaacgtgtag
    14281 aactgatttt atatcattcg acagatgaaa cagcaagtgt atcaaaagtt ctttctatga
    14341 aaagagggac agccgtcaat tataaaaatc ataaagaaaa cacccatggc gtctggttta
    14401 ctgaacttga aggaaactat aattaccaag catatactta tcgagtctac tatagacgac
    14461 gtacctttaa aattacgaga gacccttatt ccattgcaac gactgcaaat ggtaaaaggt
    14521 ctattgttat tgctccagaa gcattaactc cggaagggtt taaaataagt catggaaaag
    14581 aagctaagtg gcggctcgaa aaccccaatc aagcagtcat ttacgaaatg catgtgcgtg
    14641 atttttcgat atctgagact tcaggagtta agacagatta tcaaggaaaa tttaagggat
    14701 tgcatcaaaa agatacagtt aatcagcatg gtgataaaac gacctttgat tatgtgcaag
    14761 acttaggagt taactatatc caacttcaac caattttcga tcatcatcag acgtttgatg
    14821 atgatggtca ctatgcttat aattggggat atgatcctga aaactacaac gttccagaag
    14881 caagcttctc tagtaatcca catgaacctg caacgcgtat tttagaatta aaatctgcta
    14941 tccaagcgta ccatgatgca ggcattggtg ttattatgga tgttgtctat aatcatactt
    15001 tttcatcaac tgattctgca tttcaactaa cggttccaga ttattattat cgaatgaatc
    15061 acaatggaac atttcaaaat ggttcaggct gtggtaatga aacagctagt gaaaaagaaa
    15121 tgtgtcgtaa gtatattctt gattcagttt tgtattgggt gaaagaatat aatatcgatg
    15181 gttttcgatt tgatttgatg ggactacatg atgtagaaac gatgaacatt ataagaaatg
    15241 agctaaataa aatcgaccct cgtattttgg tttatggaga aggatgggac atgggagctg
    15301 gtctcacgcc tcaaaataaa gctaaaaaag ataatgctta tcagatgcct ggaattggct
    15361 ttttcaatga tgatgtaaga gacgctgtca aaggtgcgga aatttacgga gaatttaaaa
    15421 aaggcttagt ttctggaaac agtacagaag atattgttgc aaaaggcatt ttaggaagtg
    15481 atgagttagt atcatacatt gatcctagtc aagttttaaa ttatgttgaa gcacatgaca
    15541 actacaactt aaatgatttg ctatgggaat tacatcctaa tgacaacgag aaacaacata
    15601 tttaccgagt tgaagtagct agtgctatga atctattgat gcaagggatg gctttcatgc
    15661 aattaggtca ggagtttctt cgaacaaagt gttatcctac aggtgataaa ggtcaactta
    15721 ctcaagcaga taaagaaaga gctatgaatt cttacaatgc cccagaccaa gttaatcagg
    15781 tcaactggga taatgttacc tttcataaga gtactattaa ttttattagg aaaataatta
    15841 ctttgaagac aaactctcct tacttttctt actctagctt tgaagagatt cgcaaacatg
    15901 tttttgtaga gtctgctcaa taccattcag gtttcataag ttttacagtc gaagaacata
    15961 agaaaataaa aataattttc aatattaaag gaaaacgctt gcaggatcct ttcataggtg
    16021 atataataat atcaaatcat gaagaaggaa aagcgataga ttcattagat gatgagcttt
    16081 ctgtaatgat ccttgatatc acaaaataac ctgaaaacat tcaggttatt tttgtgtata
    16141 ttaaggaatt tgatttgaat ttgagtttct tatattttag ttaatttttc ggaaatatag
    16201 agggtgaaat attttcagat gcgtctgtat gaggaaaatg cattcctctt tttttgtacc
    16261 attgaggaaa tatttttgtc ttatattgac tagtagacct gtgaggtgag tagatgtcca
    16321 ataaagatga gttatatact tttggaatag gtgaaaattt tcatctccag aattatttag
    16381 gcgtacatag cgaaaatgga agcttttgtt tcagagtttg ggctcctaat gctgaaaatg
    16441 ttcaagttat tggagatttt acagattggc gaaatcgacc tctccaaatg aacaaaattc
    16501 aagcaggagt ttgggaagct aacagtttgg atgcgcgcga aggtgattta tataaatatt
    16561 tggttactcg taaaggtggt caagtagttg agaaaatcga tcctatggca gtttatatgg
    16621 aaagacgtcc tggaactgca agtgttatta aagttcttag aaataaaaag tgggaagatg
    16681 gtctttggat gggaaggcgc aaacgtctgg gttttcagaa gcgtccaatt aatatttacg
    16741 aagttcatgc tggttcatgg aaaaaagatg attttggaca tcctatgact ttttctcagt
    16801 taaaagatta cttaatcccc taccttgttg agatgaatta tactcatgtt gaatttatgc
    16861 ccttaatggc acatccgctt gatatgagtt ggggatatca attaatgggg tattttgctt
    16921 ttgaacatac ctatggtact ccagaagaat ttcaagactt tgtagaagca tgtcataaaa
    16981 ataatatagg agttcttgta gattgggttc caggacactt tattcagaat gacgatgctt
    17041 tagcctattt cgatggaact gctacttatg agtatcaaaa tcatgatcgt gcgcataact
    17101 atcgatgggg agctctgaac tttgatttag gaaaaaatca agtacaatcc tttttgattt
    17161 cgagtgcctt gttctggatt gaacattatc atattgatgg tattcgtgtt gatgcggtta
    17221 gtaatatgtt atatctcgat tatgatgaag gaccatggga agctaaccaa tttggggata
    17281 atcgtaattt ggaaggatat cactttttaa ggaaacttaa caaagtaatt aaagagcgtc
    17341 atcctaatgt tatgatgatt gctgaagagt caacagcttc taccccaata actaaagact
    17401 tagagtctgg aggattgggt tttgacttta aatggaatat gggctggatg aatgatatcc
    17461 tgcgttttta tgaagaagat cctctctata ggcaatatga ttttaattta gtgactttta
    17521 gttttatgta tatattcaat gagaactttg tactagcttt ttcacatgat gaagtagtac
    17581 atggtaaaaa gagtatgatg cataaaatgt ggggtgaccg ttataatcaa tttgctggtt
    17641 tacgtaattt atatgcatat caaatgtgtc atcctgggaa aaaacttctc tttatgggaa
    17701 gtgagtttgg tcaattttta gaatggaaat ataatgatca actagaatgg gaaaacttaa
    17761 atgatgatat gaaccaaaag atgcaacgct atactaaaca attgaatcaa ttttataaag
    17821 atcataagtg cctatggcgt attgatgatt cctttgacgg gctagaaatt attgatgcgg
    17881 ataataaatc tgaaactgtt ttatctttta tacgaaaaga tgataaaggc gatttgttac
    17941 tttgtgtctt taatatgact cctgtagaac gccctaactt tactattggt gttccgcaag
    18001 cgggtattta tgaagaagtt ttaaatactg aaatggaaga gtttggaggt gtttggaaaa
    18061 accacaatcc tgtaacgaaa acacaagtgg ctacatggaa agattatgat catactttaa
    18121 gttttacatt accagccctt ggggcaagtg tttggagaat aaaacgtcgg cttcgtaaat
    18181 aagtctgcaa tttagtaacc tcattttgaa aggaaagaat aatatgaaaa atgaaatgtt
    18241 agcactcatt ttcgtcggag gacaaggcac tcgactcgga aaattgactc agagcattgc
    18301 gaaacctgct gttcaatttg gaggtcgtta ccgtattatt gactttgcgc tttcaaattg
    18361 tgcgaattca ggtattaata atgttggagt tattactcag tatcaaccgc tggaactcaa
    18421 tactcatatt ggaaacggat ctagctgggg actagatggt atcgattcgg gagtaactgt
    18481 attacagcca tattctgcaa cagaaggtaa tcgctggttt caaggtacca gccacgctat
    18541 ctatcaaaat attgactaca ttgatcgtat taatccagaa tatgtactca ttctctctgg
    18601 ggaccacatc tataaaatgg actatgatga catgctacag acgcataaag ataatttagc
    18661 aagcctaaca gtcgctgttt tagatgttcc tttaaaagaa gcaagccgtt ttggaattat
    18721 gaatactgat tcaaacgacc gtattgtcga atttgaagaa aaacctgaac atcctaagtc
    18781 aacaaaagct tcaatgggaa tatacatttt tgactggaag agattacgga cagttttaat
    18841 tgatggtgaa aagaacggca tagacatgtc agactttggt aaaaatgtta taccagcata
    18901 ccttgaatcg ggtgaacgtg tttataccta taattttgat gggtattgga aagatgttgg
    18961 tactattgaa tccctttggg aagctaatat ggaatatatt ggagaagata acaaattaca
    19021 tagtcgtgat cgttcatgga aaatctattc taaaaatttg attgcaccac ctaatttcat
    19081 gacagaagat gcaaatgtta aagattcttt agttgtagat ggatgctttg ttgcaggaaa
    19141 tgttgagcac tccattttat caactaatgt ccaggtaaaa ccaaatgcta ttattaaaga
    19201 ctcctttgtt atgagtggcg cgactatcgg tgaaggtgca aagataaatc gagctattat
    19261 cggtgaggat gctgttattg gtgatggtgt tgtgattgat ggaagtaaag aagtagaagt
    19321 tattggttat aaagaagtag cgggggttcc aaatgaagat tgataaatat actgcgattt
    19381 taggaaatac tgttagttac ccagatatgc acagtttgac ggataatcgt ccgcttgctt
    19441 ctctaccttt tgatggtaag tatcgtttaa ttgattttca attatcctct ttagctaatg
    19501 caggcgttag aagtatttat ggtatttttc gtgggaataa tgttcgctct gtttttgacc
    19561 atattagaag tggtcgtgag tggggcttag gtacactgtt gagtcactat tctttaggat
    19621 tctatgatac agacgatgat actcgtacag tcaatgggga ttattacgat taaattttaa
    19681 cttacttgag gcgttcgggt agcgaccaga cgatatatat gagttgtgat atgctttgta
    19741 atattgactt aagtcaagta atccacttgc ataatgcaaa tggtgtgcaa gccaccgtgg
    19801 tttataaaaa aatgccgaga tcagctattg cagaggctaa tcaagtcttg accattgatg
    19861 aatctgatca tgtagtgagt ctaggtaaag ctgtagataa tgacgataac caaaaaatgt
    19921 ctgctgagat ttatgttatc gatacacctt ggttaatcga aaaaatggaa gaggaagctc
    19981 aaaataatga accacgtaaa ctccgcttct tacttcgtga tttaattgtt gaaagtaatg
    20041 ctttggcttt tgaatacact ggttatttgt caaacatttc ttctattaaa tcctattatg
    20101 atgctaacat ggacatgtta acaccaaata aattttattc gctcttcttt tcaaaccaaa
    20161 aagtttatac taaggtgaaa aatgaggaag ctacttattt tgataaacaa tcaaatgtct
    20221 ctaattcgca actagcctat ggaagtatta ttaaaggtta tttagaccat tctatcgttt
    20281 ctagaaattg tctccttgaa aaagggactc gagtagtcaa tagtattatc tttccaaaag
    20341 taaaaatcgg agaaggggca accattgaaa atactattat tgacaaatgc gttaaggttg
    20401 catccggagt tacattgaaa ggtagtttag ataaaccgtt agtgattcct aaattttcag
    20461 agattaatga ggatattata caatgaaaat aatgtttgtt gcggcagagg gtgctccctt
    20521 tgctaaaaca ggtggtttgg gagatgtgat tggcgcactc ccaaaatcac ttagtaaaaa
    20581 aggtcatgat gtcgctgttg tcatgccata ttatgatatg gtagaccaaa aatttggaga
    20641 ccaaatagaa aacctcatgt atttctatac agatgttggc tggcgtcatc aatatgtagg
    20701 agttaagaga ctttctcaag ataatgttac gttctacttt attgataatc aatattattt
    20761 ttatagaggt catgtctatg gtgattggga tgatggggag cgttttgctt atttccaatt
    20821 agctgcttta gagttgatgg agaaaataga ttttattccc gatgtcttac acgtccatga
    20881 ttatcataca gcaatgattc ctttcttatt gaaagagaag taccactgga ttcaggccta
    20941 taataatata agagcagttt ttactattca caatattgaa tttcaagggc agtttggtcc
    21001 agaaatgtta ggagatttat ttggagttgg cgctgagaga tatgaagatg gaactcttag
    21061 atggaataat tgtcttaatt ggatgaaagc agcaattctt tactcagata gagtgacaac
    21121 ggtatcacca agctacgcaa atgaaataaa aactccagaa tttggtaaag gtttagacca
    21181 aattatgcgt atggaagcag gtaaattatc tggaatcgta aatggtattg acagcgattt
    21241 gttaaatcca gaaacagatg cttttttgcc atatcatttt tcaaaaagca acttagaggg
    21301 caaaattaaa aataagttgg ctttacagga aaatttgggc ttgcctcaag ataaaaatgt
    21361 accattaatt ggtattgttt cacgacttac cgatcaaaaa ggatttgata ttattgcttc
    21421 tgaattggac aatatgcttc aacaagatat tcaaatggtt attttaggaa caggctatca
    21481 ccattttgaa gaaacgttct cttattttgc tagtcgttac cctgaaaaac tatctgctaa
    21541 tattactttt gacttaggat tagcgcaaca gatttatgcg gcttcagata tcttcatgat
    21601 gccaagtgct tttgaacctt gtggtctctc gcagatgatg gctatgagat atggtagctt
    21661 acccttggta catgaagttg gaggattaaa agatactgtg gtagccttca atcaatttga
    21721 tggtagtggt acaggttttt catttaatca tttttcaggc tattggttaa tgcaaactct
    21781 aaaactagca ttagaagttt ataatgatta tcctgaagcg tggaaaaagt tacaatggca
    21841 agctatgtca aaagattttt catgggatac agcatgtgtt gcttacgaac aactgtatca
    21901 gcaactacaa taatcacttt attttagtga ttagttgata agcttatcga atctctatgc
    21961 taaacgtttc ttatgtaaaa ccactttagt atagagattt tattatctta taaggtaaaa
    22021 tattattaat gtaaagagac actatatcaa aaataaagtg ttgtcatatc ttaataaggg
    22081 attaaattaa agctaaaaaa actaaaaatt gttatttgaa aaggattgtg taaaccatct
    22141 caaactattt catgaaaacg taatcaaatg aggaaattca ttgacatgca caagaaatag
    22201 gaataaaata acggagtaaa tattttttaa aggagaattc tattatgaat ttagctattt
    22261 tagcacttgg tttcgccgtg atgggtgttt caatcggtga aggaattttg gttgcaaata
    22321 ttgccaaatc agcggctcgt cagcctgaaa tgtttagcaa actgcaaaca ttgatgttta
    22381 caggtgtggc cttcattgaa ggtaccttct tcgttctatt tgcgtttaca ttcttagtaa
    22441 gatagacgac aatttagaaa aaaggaggga tgaggattgg aatctacaag taatccaacc
    22501 gtatcttttt taggcatcga ttttgattta accatcctag ctatgtctct cttgacaatt
    22561 actattatct ttatcttagt cttctgggct agtcgtaaga tgacaataaa accaaaaggt
    22621 aagcagaatg tcttagaata tgtctatgag cttgttaata atacaatctc acaaaattta
    22681 ggacattata ctaaaaatta tagtttattg atgtttattt tattcagttt tgtcttcatt
    22741 gctaacaact taggattgat gacatcattg aaaacacatg agcataattt ttggacatct
    22801 ccgaccgcta attttggtgt agatattaca ttatcattat tagtagcatt tatttgtcat
    22861 attgaaggta ttcgcaaaaa aggaattggt ggttatctca aaggtttctt atcaccaaca
    22921 ccagcaatgc ttccgatgaa tttattggaa gaagttacta atgttgcttc attagcattg
    22981 cgtctttttg gtaatatctt ttctggtgaa gttgtcactg gattactttt acaacttgca
    23041 gttttatcac catttacagg tccactagct tttgcattga atatcgtttg gacagcattt
    23101 tcaatgttta ttggtttcat ccaagcgtat gtgttcatta tcttgagctc aagttatatt
    23161 ggacacaaag ttcatggtga cgaagaagaa taagaaaaga gaggagaaat atgtcaatac
    23221 ttattaatag tacaacgatt ggtgatatca ttatcgtatc tggctctgtg cttctcttat
    23281 ttatcttaat taagactttt gcatggaaac aaatcacagg tatttttgaa gcccgtgaac
    23341 aaaagatagc taatgatatt gatactgctg aacaggcacg tcaacaagct gaagcttttg
    23401 caactaagcg tgaggaagaa ctatctaacg ctaaaactga agctaatcaa atcattgata
    23461 atgctaaaga gacaggtctt gcaaaaggtg atcaaattat ttcagaagca aaaactgaag
    23521 ctgatcgatt aaaagaaaaa gcacatcaag atattgccca aaataaggca gaagcactag
    23581 cagatgttaa gggtgaagta gcagatttaa cagttttatt agctgaaaaa attatggtat
    23641 caaatcttga taaagaagct cagagtaatc tcattgatag ctatattaaa aagctaggag
    23701 atgcctaatg aataaaaaga cgcaggctct aattgagcaa tattcaaaaa gccttgttga
    23761 agttgccatt gaacataaaa ttgttgaaga aatccaacaa gaagtagcag ctttaattga
    23821 tatttttgaa acaagcgagc ttgagggtgt cctgtcaagt cttgccgttt cacatgatga
    23881 aaaacaacat tttgtgaaga cattgcaaac atcatgttca acttatcttg ttaatttcct
    23941 tgaggttatt gtacaaaatg aacgtgaagc acttctatat cctattttga aatcagttga
    24001 tcaggaactt atcaaagtga atggtcaata tccaattcaa attacaactg cagttgcttt
    24061 gtctccagaa caaaaagaac gtctttttga tattgctaaa actaaattag ctttaccaaa
    24121 tggacagtta gttgagcaca ttgacccatc tattgttggt ggatttgttg tcaatgcaaa
    24181 caataaagta attgatgcaa gtgttcgtaa tcaattacat caatttaaaa tgaaattaaa
    24241 atagaaagtg gtgtgacttt tggcaattaa tgcacaagaa attagcgctt taattaaaaa
    24301 gcaaattgaa gacttccagc caaattttga cgtcacagaa actggtattg ttacatatat
    24361 cggtgatggt attgctcgtg ctcgtgggct agataatgct atgagtggag aacttcttga
    24421 attttcaaat ggtgcgtacg gtatggctca aaaccttgag tctaatgacg tgggtatcat
    24481 tattttgggt gatttctcag aaattcgtga aggtgatgtg gtaaaacgta ctggtaaaat
    24541 tatggaagta ccagtagggg aagctatgat tggacgtgtc gtcaatccgc ttggtcaacc
    24601 agttgatgga ctaggtgaaa ttgagacgac agcaacacgc ccagttgaaa caccagcacc
    24661 tggtgttatg caacgtaaat cagtttttga gcctcttcaa acaggtctca aagcgattga
    24721 tgctttggtt cctatcggac gtgggcaacg cgaattgatt atcggtgacc gccaaactgg
    24781 taagacgtct gtagcgattg atgcaatctt gaatcaaaaa ggtcaagata tgatctgtat
    24841 ctatgtggct atcggtcaaa aagagtctac agtacgtaca caagttgaaa ctttgcgtaa
    24901 atatggtgcc cttgattaca caatcgttgt gacagcttca gcatcacaac catctccatt
    24961 acttttcatt gctccatatg caggagtagc aatggctgag gaattcatgt ataacgggaa
    25021 acacgttttg attgtttatg atgatttatc aaaacaagcc gtagcttatc gtgaactttc
    25081 gcttcttctt cgccgtcctc caggtcgcga agcttaccca ggggatgttt tctatcttca
    25141 tagtcgtctt ttggaacgtt cagctaaagt atcagatgct ttaggtggag gttcgattac
    25201 agcgcttccg ttcattgaaa cacaagcagg tgatatctca gcatatattg ctacaaacgt
    25261 tatttcaatt actgacggcc aaattttctt gcaagaaaat ctctttaact caggtattcg
    25321 tccggcgatt gatgcaggtt catcagtatc tcgtgttggt ggtgcagcgc aaattaaagc
    25381 gatgaagaga gttgcaggta cgcttcgtct agatcttgct tcttatcgcg aacttgaggc
    25441 attcactcaa tttggttcag accttgatgc agcaacacaa gcaaaactaa atcgtggtcg
    25501 tcgcacagtg gaagtgttaa aacaaccatt acacaaacca ttgccagttg agaaacaagt
    25561 tgtgatttta tatgctctaa cacatggatt ccttgatgat gtacctgtaa atgatatttt
    25621 agcttttgaa gaagctttgt atgattactt tgatgctcat tatgataatc tcttcgaaac
    25681 aatccgcaca acaaaagatt taccagaaga agcagaatta gatgctgcta ttcaagcatt
    25741 taaagatcaa tcacaattta aataatagga gggttctaat atggcaggct ctcttagtga
    25801 aattaaagac aagattcttt ctacagaaaa aacaagtaag attactagtg cgatgcagat
    25861 ggtctcatct gccaagttgg ttaaatctga acaagctgct agagattttc aagtgtatgc
    25921 ctctaaaatt cgtcaaatta ctactaacct cttgaagtca gacttagtat ctggatcaga
    25981 taaccctatg ttagcttcac gtccggtgaa gaagacaggt tatattgtta ttacttctga
    26041 taagggttta gttggtggtt acaattcaaa aatcttaaaa gcgatgatgg acactataac
    26101 tgactatcat actgagaatg atgattatgc cattatttca attggtagtg ttggttctga
    26161 ttttttcaaa gcacgtggaa tgaatgtttc tttcgaatta cgtggtttag aggaccaacc
    26221 aagttttgat caagttggta aaattattgc tcaagcagtt gaaatgtaca aaaatgagtt
    26281 gttcgatgaa ttatatgttt gctacaatca ccatgttaac agtttaacta gccaagtcag
    26341 aatgcaacaa atgcttccta tcaaagaatt ggatgcagaa gaggcttctg aggatagagt
    26401 gattactggt tttgaacttg agccaaaccg tgaagttata ttggagcaat tattgcctca
    26461 atatactgag agtttgattt atggagctat tattgatgct aaaacagcag agcatgcggc
    26521 tggtatgaca gctatgcaga ctgcaaccga taatgctaaa aatgttatca atgatttaac
    26581 tattcaatat aaccgtgctc gtcaagcagc tatcacacag gaaattacag aaattgttgc
    26641 aggtgctaat gcacttgaat aattaattag tctatagaaa aaatagtgaa caaaggtatt
    26701 ttaaatcctt tgtatcatat gataaggaga aatacatgag ctcaggcaaa attgctcagg
    26761 tagttggacc tgtcgttgac gttgtatttg caagtggcga caaacttcct gagattaata
    26821 atgcattgat tgtctataaa aatggcgata agtcacaaaa agtagtactt gaagttgctc
    26881 ttgaacttgg tgacggcctc gttcgtacaa tagctatgga atcaactgat gggcttacac
    26941 gtggtttgga agtattagat actggtcgcg caattagtgt gccggttggt aaggatactt
    27001 tgggtcgtgt cttcaacgtt cttggagatg ctattgacct tgaagagcct tttgcagaag
    27061 atgcagaacg tcaaccaatc cataaaaaag caccatcgtt tgatgaatta tcaacatcat
    27121 cagaaatctt agaaacaggt attaaagtta ttgacttatt agcaccttac ttaaaaggtg
    27181 gtaaagtcgg gcttttcggt ggtgcgggtg ttggtaaaac cgttcttatt caagaattaa
    27241 tccacaacat cgcccaagaa catggtggta tttcagtatt tactggtgta ggagaacgta
    27301 ctcgtgaagg gaatgacctt tattgggaaa tgaaagaatc tggcgttatt gaaaaaacgg
    27361 ctatggtctt tggtcaaatg aatgaaccac caggagcacg tatgcgtgtg gcacttactg
    27421 gtcttacaat agctgaatac ttccgtgatg tagaaggaca agatgtgctt ctcttcattg
    27481 ataatatctt ccgtttcaca caagcggggt cagaagtgtc agcgctttta ggtcgtatgc
    27541 cttcagccgt tggttatcaa ccaacacttg ctacagaaat gggacaatta caagagcgta
    27601 tcacttcaac taaaaaaggt tctgttacat caatccaagc tatttatgtg ccagcagatg
    27661 actatactga ccctgcgcca gctacagcct ttgctcactt agattcaaca actaaccttg
    27721 aacgtaaatt gacacaaatg gggatttatc cagccgttga cccattagct tcaagttctc
    27781 gtgcactcac accagaaatc gttggagatg agcactatga agttgcaaca gaagtacagc
    27841 gtgttcttca acgttaccgt gagttacaag atatcattgc gattttaggt atggatgaat
    27901 tatcagatga ggaaaaaacg ttagttggac gtgcacgtcg tatccaattc ttcctttctc
    27961 aaaacttcaa tgttgcagaa acatttactg gtcaaccagg ttcatatgtt ccggttgaag
    28021 aaactgttcg cggattcaaa gaaatcctag atggtaaaca tgatcaaatt ccagaagatg
    28081 ccttccgtat ggttggtggt attgaggatg ttatcgcaaa agcagaaaaa atgaactatt
    28141 aatgaggtga tgctatggca caattgactg ttcaagtagt aacatcagat ggtattagat
    28201 atgaccatca cgctagtctc attactgtta gaacgccaga tggtgaaatg ggaattttac
    28261 caggtcacat aaatttgatt gctcccctta ttgttcatca gatgaagatt aatcgttctc
    28321 atcaagaagg tgtagactgg gtagctgtta atggtggtat aattgaagtt aatgaggatc
    28381 aggtgacaat tgttgcggat tcagcagaac gagcacgtga tatagacctt aaccgtgcag
    28441 aacgggctaa ggaacgcgct gagcgtgcat tagaaaaagc tcaaacaact cagaatattg
    28501 atgagatgcg tagagctgag gttgctcttc gtcgcgcaat taaccgtatt agcgtcggta
    28561 aaaagtagaa aaatggtcta attagttttg tgactaatta ggctttttta gtgtataata
    28621 gaaggcatga atatgatatt aaatatagta actatagtta gtaattttat ttttattgcc
    28681 cttttttatc aactttttgt agatttattt gactggtcta aaatgattaa aatgtcccct
    28741 caaaacatct caaaattaaa agtttttatt ttgttgataa gtatagttgg tggctatgtg
    28801 gttagtcatt ttttattgga agtgattcag ttgtgtcaat caatcttttg ggctctccaa
    28861 taaaggaaat attagctgaa ctatgatata atcaacttac tattgttata agaatggaga
    28921 ttcaaatgga taaaattatt gtggaaggtg ggcaaacgcg gcttcaaggt caagttgtta
    28981 ttgaaggagc taaaaatgca gttttacctc ttttagcagc cacaatttta ccaagccaag
    29041 ggaaaacatt attgaccaat gttcctatct tgtctgatgt atttacaatg aacaatgttg
    29101 ttcgtggttt agatattcaa gttgacttta attgcgataa aaaagaaatc ttagtggatg
    29161 cctcaggtga tattttagat gttgcacctt atgaatttgt tagtcaaatg agagcttcta
    29221 ttgttgtttt gggacctatt ttagctagaa atggccatgc taaggtttca atgccaggcg
    29281 gttgtacaat tggtagccga ccaattgacc ttcatcttaa aggtttagaa gctatgggag
    29341 ctacgattac acaaaatggt ggtgatatta ctgcccaagc tgaaaagctt aagggggcta
    29401 atatatacat ggattttccc tcagtcggtg ccactcaaaa cttgatgatg gctgctacac
    29461 ttgcttcagg cacaacaacg atagagaatg ctgcacgtga accagaaatt gtagaccttg
    29521 cgcagttact aaataaaatg ggagctaaag tcaagggtgc tggtacggaa acattaacga
    29581 tcattggtgt tgatgcttta catggtacag aacatgatgt tgtccaagat cgtattgaag
    29641 ctggaacctt tatggtggca gctgctatga cttctggaaa tgttctagta aaggatgcta
    29701 tttgggaaca taaccgtcct ttaatctcta aattgatgga gatgggtgtt gaagtgtcag
    29761 aggaagaaga tggtattcgt gtaaaagctg atactaaaaa attgaagcca gtgactgtga
    29821 aaacactccc tcatccagga tttcctactg atatgcaagc tcaatttaca gctctaatgg
    29881 cagtagttaa tggcgaatca actatgattg agacagtttt tgaaaatcgt tttcaacatt
    29941 tggaagaaat gcgtcgcatg ggattacaaa cggaaatttt acgagatact gcaatgattc
    30001 atggtgggag agccttacaa ggtgcaccgg tcatgtctac tgacttacga gctagtgcag
    30061 ctttaatctt ggcaggaatg gttgcgcaag ggcaaacagt agttggccaa ttaacccatt
    30121 tagaccgtgg ttattatcaa ttccatgaaa aattagcggc tcttggtgct aatattaaac
    30181 gtgtaagtga ggcgtaatat ggcatctagt ggttggaaat atgtactaaa acaaatcggt
    30241 ctaatagtat tagtcatact gttagccttg ctgtttttag cggttggatt gatgttgggt
    30301 tatagtgttt ttggtgacgg agagcatgct tactctattt tatcgctgga taaatggcaa
    30361 aatattattg gaaaattttt aggaaaatag aaagagccac tataagttat ttgatgctta
    30421 tagtggtttc ctttgagagt taattttagc agtagaataa tacaatgaca aaaaaacaaa
    30481 aacaaataac agcttcgatt gtaactttgc ttttttcatt actggcaata gggttaggtg
    30541 aaagtaaatc tttgcctaat gatcacattt taaaacaggt gacagattta attattagta
    30601 atgaatctgg caaaaatact tcccttaaaa atatatcagg aacccctagt agagagttat
    30661 ctgaatctgt tttgactagc aatgttaaaa aacaattggg aacaaatata gcttggaacc
    30721 agtcaggtgc ttttatcatt aatcaaaata aaacagattt gaatgctaaa gtgtcaagtg
    30781 caccatatgc tattaatgaa ataaagaagg ttaataacca aattgttcca actaaagcaa
    30841 atgccttatt aacaaaagca actcgtcaat atcgaaatcg agaagaaaca gggaatggta
    30901 gaacttattg gaaaccagct ggatggcatc agataaatgg attaaaaggg agctataacc
    30961 acgctgtgga cagaggccat ttaattggtt atgctttagt agggagttta agaggatttg
    31021 atgcctccac aagtaatcct aaaaatattg ccacacaagc agcttgggca aaccaagcta
    31081 atagcaacca atcaacaggg caaaattatt atgaaacttt agttcgtaaa gcattggatc
    31141 gtcataaaac tgttaggtat cgtgtgactt taatttatga cagagataat cttttatctt
    31201 cgggatcaca tattgaggct aaatcttcag atggaagttt agaatttaat gtttttattc
    31261 ctaatgtaca atcgggatta ttatttgatt atgcaacagg aaaagtgaag cagacaaagt
    31321 aatctttatc taatagtgct tgttaaagac tgaaaaattt gataaaatag tccttaattg
    31381 aatagaggtc tgtgagacta gtaaaccaag ggaagctgct tagggagttc agccgtgact
    31441 gcaagctgaa tcagtgaaag agggtggaat tcactcacaa ataggactat aattaaggtc
    31501 tgattggtcg atcagataga aaacggatgg taccgcgtgt caacgctccg attaaggggg
    31561 agtggcacgc tttttttgtt ttcaaagcta cttattttga cttggaggaa agatggattt
    31621 acaaaaacaa ttagaagagt tgaaaatatc aactcaagaa aagcttaaag aaatgactgg
    31681 gaaccacact aaagaattac aagacttacg tgtacaagtt ttaggaaaaa aaggttctct
    31741 aacagaatta ctaaaaggtc tgaaagattt atctaatgat ttacgaccag ttgttggaaa
    31801 acaagtcaat gaagtacgtg atattttaac aaaagcgttt gaggaacaag ctaaagtagt
    31861 ggaagctgcg aagattcaag cacagttaga gtctgagagt gtcgacgtta ccttgcctgg
    31921 tcgtcaaatg acattaggtc accgtcatgt cttaacacaa actagcgaag agattgaaga
    31981 tatcttttta ggtatgggat tccaagtggt tgatggtttt gaagttgaga aagactacta
    32041 taattttgaa cgaatgaatt taccaaaaga tcatccagcc cgtgatatgc aagatacttt
    32101 ttatattaca gaagaaatct tgctacgtac tcatacttcg cctgttcaag cacgaacaat
    32161 ggaccaacat gatttttcta aagggccttt aaaaatgatt tctcctggac gtgttttccg
    32221 tcgtgatact gatgatgcta ctcatagcca tcaattccat caaattgaag gcttagttgt
    32281 tggagaaaat atttcaatgg gcgatctcaa aggaacactt caactgattt ctcaaaaaat
    32341 gtttggagca gagcgtaaaa tccgacttcg tccttcatat ttcccattca cagaaccttc
    32401 agttgaagtt gatgtctctt gcttcaagtg cggaggtaag ggatgtaatg tctgcaaaca
    32461 gacaggttgg attgagatat tgggtgctgg tatggttcac ccaagtgttt tggaaatgtc
    32521 tggtattgat tctgagaaat actccggttt tgcctttggc ttaggtcaag aacgtattgc
    32581 aatgttacgc tacggtatca atgacattcg tggcttctat caaggtgatg ttcgcttcac
    32641 agatcaattt taagatttta gatagcaagt agcatcattg gtgtgatgat aaatttatgt
    32701 taacgctatt accttactca ataggaggtt ttctaatgtc tcaaattact tttaaaaagg
    32761 ttggccttga taatgtgaat attttacaga atcttgctat agaaaccttt cggcagacgt
    32821 tttctcatga taatagtgag gaacaacttc aagcattttt caatgagtct tacactttac
    32881 cagtattaaa atcggaaatt acccatgctg aatctgatac ttattttgtt tatcttgata
    32941 cagatttagt gggttattta aaagtaaact ggggaagtca gcaaactgaa aaaaacttga
    33001 taaagcattt gaaattcaaa ggatttatct tctagatgcc taccaaggga aaggtatagg
    33061 taaggctact ttcgaatttg ctttggattt agcttataaa tcaggcttag attgggcttg
    33121 gttaggtgtt tgggagttta atcataaagc tcaggcattt tacgctaaat acggatttga
    33181 aaaattttct gagcaccaat tttcagtagg tgataaagtg gatacggatt ggttattaag
    33241 aaaaagttta cactaaaaac tacaaagcac ttgtataaaa ataaaagaaa ataaatagag
    33301 agaaattaca tgttagtatc gtataaatgg ttaaaagagt tagtggatgt tgatgtcaca
    33361 acagcagaac tagctgagaa aatgtcaacc actggtattg aggttgaagg tgtggagaca
    33421 ccagcggaag gtttatcaaa attagttgta ggtcatattg tttcgtgtga agatgttcca
    33481 gatacccact tacatttgtg tcaagttgat acaggggacg atgaacttcg tcaagttgtc
    33541 tgtggtgctc caaatgtcaa gactggtatt aatgttatcg tagcggttcc tggtgctcgt
    33601 attgcagata actataagat taaaaaaggt aaaatccgtg gtatggagtc acttggaatg
    33661 atttgttcac ttcaagagtt gggcttatcg gaaagtatca ttccaaaaga gttttctgat
    33721 ggtatccaaa ttttacctga gggagctata cctggtgata gtattttctc ttatcttgac
    33781 ttagatgatg aaattattga attatcaatt acaccaaacc gtgcagatgc tctttcaatg
    33841 cgtggtgttg cccatgaggt agcagcaatt tatgggaaaa aagttcattt tgaagaaaaa
    33901 aatctcattg aagaagcaga aagagcagct gataagattt ctgttgtcat tgaatcagat
    33961 aaagttttat cttactcggc tcgtattgtt aagaatgtaa cagttgcacc aagtccacaa
    34021 tggcttcaaa ataaactgat gaatgctggt atacgtccaa ttaataatgt tgttgatgtg
    34081 acaaactatg ttcttttgac ttatggtcaa ccaatgcatg cttttgattt tgataaattt
    34141 gatgggacaa ctatagtagc acgtaatgct gagaatggtg aaaagttaat tactcttgat
    34201 ggtgaagagc gtgacttaat agctgatgac ttggtcattg cggtaaatga tcaaccagtt
    34261 gctttggcag gcgttatggg aggacaatca actgaaattg atagttcttc taagacagtg
    34321 gtccttgaag cggcagtatt taatggaaca agtatccgca aaacaagtgg aagactcaac
    34381 cttcgttcag agtcatcttc tcgctttgaa aaaggtatca attatgatac ggtttcagag
    34441 gctatggatt ttgcggctgc aatgttacaa gaattggcag gcagtcaagt tctttctggg
    34501 caagttacag aaggagttct tccaactgag ccggtagagg tatcaacgac tcttggttac
    34561 gttaatactc gtttgggaac agaacttacc tatactgata ttgaagaagt atttgaaaaa
    34621 ctcggctttg ctatttctgg ttcagaagtg aaatttactg tgttggtacc gcgtcgtaga
    34681 tgggatattg ccattcaagc tgacttagtt gaagagattg cccgtattta tggttacgaa
    34741 aaattaccaa caacattgcc agaagccgga gcgactgcag gagaattaac gagtatgcaa
    34801 cgcttacgtc gtagagtccg tacggttgct gaaggagtag gattatctga aattatcacc
    34861 tatgctttaa cgactccaga aaaagcagtg cagttctcaa ctcaagcaac taatataact
    34921 gaacttatgt ggccgatgac tgttgatcgc tcagcacttc gccaaaatgt tgtttcagga
    34981 atgcttgata caattgctta taacgttgct cgcaaaaaca gtaaccttgc agtttatgag
    35041 ataggaaaag tttttgaaca aactggtaat ccaaaagaag atttaccaac tgaagtagag
    35101 acgtttactt ttgccttaac aggtctagta gaggaaaaag atttccaaac aaaagccaag
    35161 ccagttgatt tcttctacgc caaaggtatt gtagaggcgc tctttattaa attaaaatta
    35221 gatgttactt ttgtagcaca aaaaggttta gcaagtatgc acccaggtcg tacagcaact
    35281 atcttattag atgggaaaga gattggtttt gttggtcaag tacacccaca aactgcaaaa
    35341 caatatgata ttcctgaaac ttatgttgct gaaatcaatc tatcaactat tgaaagtcaa
    35401 atgaaccaag cgttgatttt tgaagatatt acgaagtatc cgtctgtatc tcgcgatatt
    35461 gcattattac ttgcagaatc tgttagtcat catgatattg tgttagcaat tgagtcatca
    35521 ggggtaaaac gtctaacagc gattaaactg tttgatgtct acgctggaaa taatattgca
    35581 gaagggtata aatcaatggc ttatagctta actttccaaa accctaatga caatctaacg
    35641 gacgaagaag tggctaaata tatggaaaaa atcactaaat cactcgttga gaaagttaat
    35701 gcagaaataa gataatttat atcttacgtt gaaaaataaa taaaaaacag catcaattaa
    35761 gatgctgttt ttttatttaa aatttataga tgttctgcgg agaaagtatc accgtgttgg
    35821 atgtcaccat attgaaagcc tttgttaaac cagcgttggc gttgttgagc tgttccatgg
    35881 gtaaagctat caggcactaa ttttccgtag gtttctttct gaagggtatc gtctccgacg
    35941 gcgtgggcag cattcatggc ctcttcaaag tctccttgtt ctaagagatt ttttcccctg
    36001 atgtagtgag cccatacccc tgcataataa tctgcttgaa gttctagccg aacatttaaa
    36061 gcatttgctt ctttcttagt aagtccgtgt cgcattctat tatacttatc cataatgcct
    36121 aactctgttt gaatgtggtg accaacttca tgggcgatga cgtaggccat agcaaaatca
    36181 ccagtagcac catatttatg tgataattca ttgtaaaaag aaatatcaag atagattttt
    36241 ttatctgctg aacaataaaa tggttctgaa gcagattcac ctataccaca acctgtttga
    36301 atcgaattgg tgtaaagaac aagttttggt tccttataat ttccaaaacc ttgggtttgg
    36361 aattcttgtg accagaaatc ctcagttgat ccaaggactt tattaacgaa atcgatttgt
    36421 tctctcgttg cgctattatc aacagaacgt gagacattct gagattggta actagaaggt
    36481 gaggatgagt cattaaaaat gctggttagt ccccctccgc caagtagcag taagatgatt
    36541 aaaaccacaa gcttggtttt ccaactccct cgcatcaata aaagttgaag aatcggtaat
    36601 ccacttcctc cgctagagaa tgaacctccg ctactggagc gacgatcttc aacattgtcg
    36661 ctttttctta ggtcatctat tttcataagt atctccaaag tgttttatag gctcattata
    36721 aactatttta ataagaaatg cttgaatttt taactaaata tcttccccta gatttgaagc
    36781 tatttttggt ataatattaa taaactgtaa agaataagtt gaagaaggtt tatttctcag
    36841 ttgacttata agataggaga ttcatgaaat tactttatac tgatattaat catgatatga
    36901 cagaaatctt agtcaatcag gccgcgcatg ctgctgaagc tggatggcgt attttttata
    36961 ttgctccaaa ctctttatct tttgaaaagg agcgtgcagt attagagacc cttcctcaag
    37021 aagcctcttt tgctattaca attactcgct ttgctcaatt ggcacgttat tttactttaa
    37081 accagcctaa tcaaaaagaa agtttaaacg atattggatt agcaatgatc ttttatcgtg
    37141 ctttagcaag ttttgaagat ggacaattaa aagtttttgg acgattgaag caagatgctt
    37201 cctttatcag tcaattggta gacctataca aagaattgca gacagcaaat ctgagtattt
    37261 tagatttaaa atatttacat tctcctgaaa aatttgaaga tctattagct atttttctag
    37321 tggtttcaga tcttttaaga gaaggagaat acgacaatca atctaaaatt gcctttttta
    37381 cagaacaagt aaggtcagga cagctagatg tggatttaaa gaacactatc cttatagtcg
    37441 atggttttac ccgtttctca gctgaagaag aagctttaat taaaagtctg agttcacgtt
    37501 gtcaagagat tatcatcgga gcttatgcta gccaaaaagc gtataagact aactttacca
    37561 atggaaatat ttattctgca ggggttgatt ttttaagata tctagcaacg acatttcaaa
    37621 caaaaccaga atttatcttg tctaagtggg aaagtaagtc tggttttgaa atgatttcga
    37681 aaaatatcga aggtaaacat gattttacaa atagtagtca cattttagac gacactgcta
    37741 aagattgtat aacaatttgg gagtgcatta atcaaaaaga tgaagtggaa catgtggcta
    37801 gagctatacg tcagaaactt tatcagggat atcgctacaa agatatttta gttttactag
    37861 gagatgtaga tagttataaa ctacaattaa gtaagatttt tgaacagtat gatattcctt
    37921 attatttcgg taaagctgaa acaatggctg cgcacccttt agttcatttt atggattcct
    37981 tgtcgcgaat aaaacgttat cgatttagag ctgaagatgt tttaaatctt tttaagacag
    38041 gtatttatgg agagattggt caagatgatt tagattattt tgaagcttat atctcatatg
    38101 cagatattaa aggtcctaag aagttcttta cagattttgt tgtgggggct aaaaaatttg
    38161 atttagggcg tcttaacact attcgtcagt ccctccttac tccactggaa agttttgtta
    38221 aaacgaaaaa acaagacggt ataaaaacac tcaaccaatt catgtttttc cttactcagg
    38281 taggtttgag tgacaattta tcgcgattag ttggtcaaat gtctgaaaat gaacaagaaa
    38341 aacatcaaga agtttggaaa acatttacag atattttaga gcagtttcaa acaatatttg
    38401 gtcaagaaaa attaaattta gacgaatttt tatcgttatt aaattcaggt atgatgcaag
    38461 ccgagtatcg tatggtccct gctactgttg atgtggttac tgttaagtct tacgacttgg
    38521 tagaaccaca tagtaatcaa tttgtctatg ccctaggcat gacacaatca cattttccaa
    38581 agattgctca aaacaaatct ttaatatctg atattgaacg tcagcttata aatgatgcca
    38641 atgacactga tggtcatttt gacattatga cgcgagaaaa tttgaagaaa aaccattttg
    38701 ctgctctctc tctttttaat gctgctaaac aagcattagt gttgactata ccacaattat
    38761 tgaatgagtc tgaagatcag atgtcaccat atcttgtcga actaagagat ataggtgttc
    38821 cttttaatca taaaggacgc caatcgttaa aagaagaagc tgataatatt gggaattaca
    38881 aagctttatt atcgcgtgtt gttgacttgt atcgctctgc tattgacaaa gagatgacta
    38941 aagaagaaca aactttttgg tcagttgctg ttcgttattt gcgtcgtcaa ttaacttcta
    39001 aagggatcga gataccaata attacagata gtcttgatac tgtgacagtt tcatcagatg
    39061 tgatgaccag acgcttccca gaagatcacc cacttaaatt atcctcatct gctttgacaa
    39121 ctttttataa taatcaatat aagtattttt tacaatatgt gttaggtttg gaagaacaag
    39181 actcaattca tccagatatg cggcaccacg gaacgtatct tcatcgtgtt tttgaaattc
    39241 tgatgaaaaa ccaagggata gaatcttttg aagagaaact caactctgca attaataaaa
    39301 ccaatcaaga agatgttttc aaatccttgt attcagaaga tgcagaaagt cgttattctt
    39361 tagaaatttt agaagatatc gcgcgtgcaa ctgcaaccat attacgacaa gatagtcaga
    39421 tgactgttga aagtgaagaa gaacgatttg aactaatgat tgataatact ataaaaatta
    39481 acggtattat tgaccgtatc gatcgtttat ctgatggttc tttgggtgtc gtggactata
    39541 aatcaagcgc tcaaaaattc gatatccaaa aattttataa cggtctaagt ccacaattag
    39601 tcacctacat tgatgctatc agtcgtgaca aggaagttga acaaaaacca ccgatttttg
    39661 gagctatgta cttacatatg caagaaccta gacaagactt gtctaaaatt aaaaatttag
    39721 atgatttagt aacaaaaaat catcaagctt taacttataa aggattattt tcagaagccg
    39781 aaaaagaatt cttagcaaat gggaaatacc atctcaaaga ttccctttat tcagaggctg
    39841 aaattgctat tttacaagca cataatcaat cgctttacaa aaaagcatct gagactatta
    39901 agtcaggtaa gtttttgatc aatccatata cagaagatgc taaaacagtt gacggtgatc
    39961 agtttaagag tattacgggg ttcgaggcgg ataggcatat ggcacgtgcc agagcgcttt
    40021 ataaattacc tgctaaggaa aaacgtcaag gtttcttaac attaatgcag caagaggagg
    40081 aaaatgatga cctttaagcc tttcttaaat ccagaagata ttgctgttat tcagacagaa
    40141 gaaaaaaatt ctgataaaaa acaaaagcga acgcctgaac aaattgaagc aatttatact
    40201 tttggtaaca atgtcctggt atcagcttcg gctggttctg gaaagacttt tgtaatggtt
    40261 gagcggattt tagataaact tttaagaggg gttccgattg agtctttgtt tatctcaaca
    40321 tttacagtaa aagcggcagg tgagctcaaa gaacgtttag aaaagaaaat aaacgagtct
    40381 ttgaaatccg cagaatcaga tgatttaaaa cagtttctca cccagcaact tgtcggcatt
    40441 caaacagctg atattggtac tatggatgct tttacgcaaa aaatagttaa ccaatatggt
    40501 tatactttgg gcatatcacc tatatttagg attttacaag ataaaaatga gcaagacgtc
    40561 ataaagaatg aggtctatgc tgatttattt tctgactaca tgactgggaa gaatgcagct
    40621 tcattcataa aattagtcaa aaatttctca ggtaatcgta aagatagcaa agcttttcga
    40681 gaaatggttt ataaagttta tgcttttagc cagtccatcg ataaccccaa gcgctggatg
    40741 caaacagttt ttctaaaagg tgcacaaact tacactgatt ttgaagctat tccagatcaa
    40801 gaagtttcaa gccttttaaa tgtaatgcaa acaactgcta atcaattacg tgatttaacg
    40861 gatcaagaag attataagca attaactgct aaaggggttc cgacagcaaa ttacaaaaaa
    40921 caccttaaaa taattgaaaa tttagtccat tggtctcaag actttaattt gctgtatgga
    40981 aaaaaaggat tggctaattt ggcacgtgat atcactaatg tgattccttc aggcaatgat
    41041 gttacagttg ctggcgttaa atatcctatc tttaaacaat tgcataatcg tatcgttgga
    41101 cttaagcact tagaagttat ttttaagtat caaggagaat ctcttttctt gttagaatta
    41161 ttgcaatcct ttgttttaga tttctcagaa caatatttac aggagaagat tcaagaaaat
    41221 gcttttgaat tttctgatat tgcccatttt gcaattcaaa tattggaaga aaatcatgat
    41281 attaggcaaa tttatcaaga taagtatcat gaagtaatgg ttgatgagta tcaggacaat
    41341 aaccataccc aagagagaat attagaatta ttgtctaatg gacataatcg tttcatggtt
    41401 ggagacataa aacaatcaat ttaccgtttt cgtcaggctg atccacaaat ttttaatgat
    41461 aaatacaaag cttaccaaga caatccaagt caagggaaat taattattct gaaagagaat
    41521 tttcggagtc aatcagaggt tttagacagt acaaatagtg tgtttacaca tcttatggat
    41581 gaagaagtag gagacattct ttatgatgaa agtcaccaat taaaagctgg tagtccaaga
    41641 cagcaagaaa gacatccgaa taataaaaca caagttttac tattagatac tgatgaagat
    41701 gatatagatg acagtgatag tcaacaatat gatattagcc cagctgaagc aaagttagtc
    41761 gcaaaagaaa ttattcgatt gcataaagaa gagaatgttc cctttcaaga tatcaccctc
    41821 ttagtttctt caaggacaag gaatgatggt atcttacaaa cttttgaccg ttatggtatt
    41881 cctttggtaa ctgacggtgg ggagcaaaat tacttaaaat cagtcgaagt gatggtaatg
    41941 cttgatactt tgcgatcaat tgataatccc ttaaatgatt atgctctagt tgctcttttg
    42001 agatctccca tgtttggctt taatgaagat gatttaacaa gaattgctat tcatgatgtc
    42061 aaaatggctt tttatcataa agtgaaattg tcttatcata aagaaggtca tcatagtgat
    42121 ttaattactc cagaattatc ttctaaaata gattacttta tgaagacatt ccagacttgg
    42181 cgtgattttg ctaaatggca ttcgctttat gatttaatat ggaaaatata taatgaccgt
    42241 ttttattatg attatgtagg agctctgcct aaagctgagc aacgtcaagc caatctatat
    42301 gccttggcac ttcgagcaaa tcagtttgaa aaaacaggtt tcaagggttt atcacgtttt
    42361 attcgaatga ttgataaagt tttagaaaat gaaaatgatt tggcagatgt tgaggtggca
    42421 ttaccgcaaa atgctgttaa cctaatgact atacacaaat caaagggtct tgagtttaag
    42481 tatgtattta ttttaaatat tgataagaaa ttttcaatgg tagacataac ttcccctctt
    42541 attttaagcc gaaatcaagg aattggtatt aaatatgttg ctgatatgcg acatgaactc
    42601 gaagaagaga tactgcctgc agtaaaggtt agcatggaaa cacttcctta ccaacttaat
    42661 aaaagagagt tacgcttagc tactttatca gagcaaatgc gtttacttta cgtagctatg
    42721 acacgtgcag agaaaaaatt atatctggtt ggtaaggcta gtcaaaccaa atgggctgat
    42781 cattatgact tagtatctga aaataattat ctcccattag ctagtcgtga gacttttgtg
    42841 acctttcaag attggttact ggctgttcat gaaacctata agaaacaaga gttgttttat
    42901 gacatcaact tcgtatcatt agaggaatta acagatcacc acatagggat ggttaatcct
    42961 agtctacctt tcaatcccga taataaggca gagaatcgtc agtctgaaga tatcgtgcac
    43021 gcgattagcg tgttagaatc tgttgaacag attaatcaaa cttataaagc ggctattgag
    43081 ttaccgacag taagaacacc aagtcaagtc aaaaaattct atgagcctat tctagatata
    43141 gaaggcgttg atgtgatgga aactattact aaaacatcag tagattttaa gttacctgat
    43201 ttttctacaa gtaaaaagca agacccagca gctttaggat ctgctgtaca tgaattaatg
    43261 caacgcatag agatgtcatc tcatgtcaaa atggaagata ttcaaaaagc cttgacagaa
    43321 gtgaatgctg aaacaagtat caaagcggct attcagattg aaaaaatcaa ctattttttc
    43381 caagaaaact cactcggtaa atatatacaa gaagaagtag aacatctaca tcgagaggca
    43441 ccttttgcca tgttaaaaga agatccggag agtggtgaga aattcgttgt acgtggtatt
    43501 attgatgggt atctactatt agaaaatcgg ataattctat ttgattataa gactgataaa
    43561 tttgtcaatc ctctggaatt aaaagaacgc tatcaggggc aaatggcttt atatgcagaa
    43621 gctttaaaaa aatcttacga gatagaaagg atagacaaat acttaattct tttaggtggt
    43681 aaacagctag aagtggtaaa aatggattaa agaaaggaaa aaatgatagt cgaacaaaaa
    43741 tttggaaatg gttttacttg gattaacatt gaagcagagc aattgcgaac agaaacatcg
    43801 gaaattcaag caaaatattt ggatagtgag attattacat atgctctgga tgattatgaa
    43861 cgagccttta tggaatgttc tcatattaaa ggaaaagaag tcttgacgat tatttttaat
    43921 actattgatc taaagcagaa agaaagttat tatgagaccg ttccgatgac cttttgctta
    43981 tcacacgacc gtttgataac ggtgacacgg tctcgaaata gttatatgct agaactattg
    44041 caaaaatatc tagaccgtaa tccagatgtc tctcctaaaa aatttttatt tgcagcttta
    44101 actttgatta caaaacaata ttttaatgtt gtatcgaaaa ttgatagaga aaaggatatt
    44161 ctcaaccgtc aattgagaga acaaacaacc aataagcgct tattagccat gagtgaccta
    44221 gagacaggct ccgtttatct tttgacagct gccaatcaaa atgcattggt attagagcaa
    44281 ttagatgttc atcctagtca gcgatttaat tctgaagtgg aaaaagaaca gttatcagat
    44341 gctctcattg aagcgcatca gttagtcagt atgacacaat taaacagtca ggtactttca
    44401 caattatcat ctacttttaa taatgtctta aataataact tgaacgaaaa cttaacaggt
    44461 ttgaatatca tttcgattaa tttagcgatt attgcagcaa ttactggctt ttttgggatg
    44521 aatattccat tacctcttac agaatcacgg agttcatggt taattgttat tgctacttcg
    44581 gtcttacttt gggttattat tgctcaaatt ttaaaacgag tttttaactt aagaaaataa
    44641 aaatgttcag tgcgtaagca ccgaacattt tttatttacc taagcagaat tggctgaaga
    44701 gctgagttat tagctcatct ggagctgcat ccccagtgat ttctccaagt atttcccaag
    44761 ttctagtcat atcgacttgt aagagatcaa caggcattcc taattccaag ccctcattga
    44821 cagcttttaa actatcaacg gctttttcaa ttagtgagat atggcgagca ttcgatagat
    44881 aagtagcgtc atgttctacc ataccagcat tgtcaaagaa aatatcgtta atacgttctt
    44941 cgattttatc gatgttttca ttttctaaaa ccgaaattgg aataacattt tcaggtaatt
    45001 cgttaacttc tattttttga ggaagatccg ttttattgag taatacgata cgattacttt
    45061 ctttacttag ttctagaagg gagcggtctt gtaatgttag tggttcggaa ctattgagaa
    45121 ccaacaacac aaggtcggct tcttctaaag ccttctttga acgttcaaca ccaatttttt
    45181 caacgatatc atctgtatca cggataccag cagtgtcaac tagttttaaa ggaactcctt
    45241 taatattgac gtattcttca ataacatcac gagttgttcc ttcaatatct gtcacaatcg
    45301 ccttttcttc acgtaagagg ttatttagta agctggattt accgacattt ggacgaccaa
    45361 tgattgctgt tgaaagtcct tctcgaagaa tttttccatg tcgggcagtt cgaagtaaat
    45421 tttccataag tgcttgaaac tcttgtgttt tttcacgcat cagtgttgta gtcatctctt
    45481 caacgtcatc atattcaggg taatcgatat taacttcaac ttgggctaga gtatttagaa
    45541 tttcttgtcg agtgttattg attagtgtct ttaaagaacc atctaattgc ttaacggcaa
    45601 tatccattgc tttatctgtt ttagctctaa taagatccat aacagcttca gcctgagtca
    45661 agtcaactcg accgtttaaa aaagcacgtt tagtaaattc tcctggttca gccattcgtg
    45721 ctccatgtcg taaaattaac tgaagaattt cgttagtaac tgcaattcct ccatgcgtat
    45781 taatttcaat aacatcttct cttgtgaatg tttttggagc aagcataacg cctaacataa
    45841 cttcatccaa gatttcattt ttatctgggt ccacaatatg accatagttt aaggtatggg
    45901 attggatagc ggatagatct tttccacgat aaatttttga tgctatcttt aaggcatctg
    45961 taccagatat acggacaata ccaatagctc cctcaccgag aggagttgaa atagctgcga
    46021 tggtatcaaa ttcttttgta atagacataa agtctctctc tttcttggga attactaaca
    46081 ttcattgtaa cctaaagccc taaaaggtgc aatcatttga ttaaaagaga agatttccat
    46141 aatggtttta tttgaaggta tgctataata aaactatgat tatactacaa ggaaacaaaa
    46201 ttgagcgctc attttcaggt gatgtgcttt ttgataatat taatattcaa gttgatcaac
    46261 gtgatcgtat tgctcttgtt ggacgtaatg gtgctggcaa atcaacgctt cttaaaattt
    46321 tagttggtga agaggctccg acaaaaggag aaataaataa aaagcgtgat ttgagtttgt
    46381 cctacttggc acaagatagt cgttttcaat ctgaaaatac aattttccaa gaaatgttac
    46441 aagtttttga ttcattaaga gaagtagaga agcgcttacg tgaattggaa ttacaaatgg
    46501 gacaagtttc aggaagtgac ctggaacaat tgatgaaaac atacgatatt ttgtcagaag
    46561 agtttcgtga aaaaggtggc tttacctatg aatcagatat aaaggctatt ttaaatggct
    46621 ttaagtttaa cagtgatatg tgggaaatgc ctattagtga attgtctggt gggcaaaata
    46681 cacgattagc gcttgcaaaa atgctacttg aaaagccaga attacttgtt ttagatgaac
    46741 caacgaacca tttagacatt gatacgattg cttggttaga aaattatctt gtaaattatc
    46801 aaggagccct tattattgtc agccatgacc gctatttttt agacaaggta gctacagtaa
    46861 cttatgattt aacgacacat tctctagacc gctatgtagg caattattcg aaatttatgg
    46921 atcttaaggc agagaaaata gcgactgaag aaaagaattt tgaaaagcaa caaaaagaaa
    46981 ttgctaagtt agaagatttt gtacaacgta acattgttag ggcttcaacc actaaacgag
    47041 cacaagctcg tcgtaaacaa ttagaaaaga tggaacgact cgacaaaccg aatgtagagc
    47101 aaaaatcagc taatatgact tttcatgctg ggaaagtctc agggaatgtt gttttaacac
    47161 ttgagaatgc agctattggt tatgaaggtg tttctttatc ggagccaatc gatttggacg
    47221 tgaagaaatt tgatgctatt gcaattgtag gtccaaatgg tatcgggaaa tcaactttaa
    47281 tcaaatcgct tgttgggcaa attcctttta tcaaaggaga agcaaaatta ggagcaaatg
    47341 tcgaaactgg gtattacgat caaagtcaaa gtaatttaac aaaaacgaat actgttttag
    47401 atgagttatg gaatgctttt tcaacaactc cagaagttga aattagaaac cgtttaggag
    47461 cttttctttt ttcaggtgat gatgttaaaa aatcagtttc aatgctatcc ggtggtgaac
    47521 gagcgcgttt gctattggca aaattatcta tggaaaataa taatttcctc atattagatg
    47581 agccaacaaa ccacttggat attgatagta aagaagtctt agaaaatgca ctcatcgaat
    47641 ttgatggcac attacttttt gttagtcatg atcgctattt tattaatcgt gtggcaacta
    47701 aggttctcga aatttctgat aagggaagta ctctctatct aggagattat gattattacc
    47761 tcactaagaa agccgaacta gaggaattag cccgtttaaa tgaagaagaa gtgagtgctt
    47821 caaaaacaga aatcgacgtt acatcagatt atgagacaca aaaagcaaat caaaaggaat
    47881 ttaggaaaat tacccgtcgc gttgcagaaa ttgaggcacg tttggaagtt cttgaaaatg
    47941 atgaaaacaa tatcaatggt ttaatgttag aaaccaatga catcggaaaa ctctctgatt
    48001 tacaaaaaga gctggaatca atccaagaag aacaattgct attaatggaa gaatgggaaa
    48061 atctaaatat gagactagat ttgtgagaaa atttcaaaaa caatagtttg tgaaaaaaag
    48121 tacattttaa attgagatgc cttttacgaa aacaaagaaa gcggttcaaa ttcttgcatc
    48181 ttgtgaaaaa aacttataca atgattaagt aataaagatt tttttggagg ttagaatggt
    48241 aactctatct aaagaacaac acttggatat gtttctaaaa atgcaacgta ttcgtgatgt
    48301 tgatatgaaa tttaacaaat tggttcgtcg tggtttcgtc caagggatga ctcacttctc
    48361 tgttggtgaa gaagctgctt cagtaggtgc tatccaagat ttaacagatt cagatatcat
    48421 tttctcaaac caccgtggac acggacaaac tattgcaaaa ggtattgata ttggtggaat
    48481 gtttgctgaa cttgctggta aagcaacagg aacatcaaaa ggtcgtggag gctcaatgca
    48541 cttagcaaac cttgaaaaag gtaactatgg aacaaacggt atcgtcggtg gaggatatgc
    48601 gcttgcggta ggtgctgccc ttacacagca atatgaagga actgataaca ttgttatcgc
    48661 cttttcgggt gactcagcga ctaacgaagg ctcattccat gagtcagtta acttagctgc
    48721 tgtttggaac ttaccagtta tcttctttat catcaacaac cgttatggta tctcaacaga
    48781 catcacatac tcaactaaaa ttcctcacct ttacatgcgt gctgatgcgt acggtattcc
    48841 agggcattat gttgaagatg gtaatgatct tatggctgtt tacgagaaaa tgcatgaagt
    48901 tatcaattac gtgcgttcag gaaatggacc agctattgta gaggttgaat cataccgttg
    48961 gtttggtcat tcaactgctg atgctggggt ataccgtact aaagaagaag ttgattcatg
    49021 gaaagctaaa gatccagtaa aacgctaccg tgcatactta attgaaaatg aaatcgctac
    49081 tgaagaagag cttgcagcta ttgaagcaca agtgattaaa gaagttgaag aaggggttaa
    49141 attcgctgaa gaaagtccat tccctgatat gtctgttgca tttgaagacg tttttgtgga
    49201 ctaaaataac ctaaagtaga tgagatttat ctcgttctat tattcaattg attaaaaaac
    49261 ggatattcgg aggaaataat gagcgaaact aaagttatgg ctttgcgcga agcgattaat
    49321 gtcgctatga gcgaagaaat gcgcaaagat gaaaaagtat ttttaatggg tgaagatgta
    49381 ggtgtatatg gaggagactt cggtacatct gttggtatgt tagaagagtt tggtgctaag
    49441 cgcgttcgcg acacaccaat ctcagaggct gcgattgcag gttcagctat cggtgctgca
    49501 caaacaggtt tgcgtcctat cgttgatttg acattcatgg atttcgtaac aatcgcaatg
    49561 gatgctatcg ttaaccaagg tgctaaaacg aattatatgt ttggtggagg tctatcaact
    49621 ccagttactt tccgtgtggc atcagggtca ggtattggtt cggctgcaca acactcacaa
    49681 tcattagaag cttggttaac acacattcca ggtctaaaag ttgttgcacc tggtactgtt
    49741 aatgagtcaa aagcactttt gaaatcttct attcttgaca ataacccagt tatcttctta
    49801 gagccaaaag ctctctatgg taagaaagaa gaagttaata tggatcctga tttctatatt
    49861 ccacttggta aaggggatat taagcgtgaa ggtactgact taacaattgt ttcttatggc
    49921 cgtatgcttg agcgtgttat gcaagctgct gaagaagttg ctgaggaagg tattaacgtt
    49981 gaggttgttg atccacgtac acttattccg cttgataaag aattaattat cgattctgtt
    50041 aagaaaactg gtaaattgat cttggttaac gatgcttata aaactggtgg tttcactggt
    50101 gaaattgcaa ctatggttgc tgaaagtgaa gcatttgatt accttgatca tcctatcgtg
    50161 cgtcttgcaa gtgaagatgt tccagtaccg tattcacgtg tacttgaaca aggaatctta
    50221 cctgatgtag caaaaattaa agatgctatc tacaaagtag ttaacaaagg taaataattc
    50281 ataaaacaaa gagggactga ggaatgagtg aaaacttagc ctctttcctt gtttataggt
    50341 tttaatttat atcgttagat gaaacatata tttgacgata tagtttagtg tgaaaggaaa
    50401 actatggctg tcgaaattat tatgcctaaa ctcggggttg acatgcaaga aggcgaaatc
    50461 cttgagtgga aaaaacaggt aggtgatgtt gttaatgaag gtgacgtcct acttgaaatt
    50521 atgtcagata aaacaaatat ggagattgaa gcagaagact caggtgtttt actaaaaatc
    50581 acacatggga atggagatgt tgttcctgta actgagacaa ttggctgtat cggtgctgaa
    50641 ggtgaagaag taacagaagc atcttcttct gaaaatacat cagttgaaga aaacgcgaca
    50701 caagtaactt cagaaccaga aaaagttgaa gaaacatcag aaccaagtgt gccagcagct
    50761 acaagtggtg aaaaagtacg tgccactcct gcagctcgta aattagcaag agagatgtct
    50821 attgaccttg cactagtatc tggaacaggg gcaaatggtc gcgttcacag agaagatgtt
    50881 gaaagcttca agggtgctca accacgtatt acaccattgg cacgtcgaat tgctgaagat
    50941 caaggagttg atatagcaga aatcactggc agtggtattc gtggtaaaat tgttaaaaat
    51001 gatgttttgg ctgctatgtc accgcaagca gcagaggctc ctgtaaaaac gaaagctaca
    51061 ccaacaacag aagaaaaaca attaccagaa ggtgttgaag tcattaagat gtctgcaatg
    51121 cgtaaggcta tttctaaagg tatgactaac tcttacctca ctgctccaag ctttacactt
    51181 aactatgata ttgatatgac tgaaatgatg gctcttcgca agaaattaat tgatccaatc
    51241 atggctaaaa caggcttgaa agtcagcttt actgatttga ttggaatggc cgttgttaag
    51301 acattgatga aaccagagca tcgataccta aatgcttcat taattaacga tgcacaagaa
    51361 attgagcttc ataattttgt taatattgga atcgcagttg gtcttgatga tggtttgatt
    51421 gttcctgttg ttcacaatgc tgatcaaatg tcactttctg actttgttat agcatctaaa
    51481 gatgttatta agaaaactca agaaggtaaa ttaaaatcag ctgaaatgtc aggttcaaca
    51541 ttctcaatta ctaacttagg tatgtttggt actaagacgt ttaacccaat tatcaatcaa
    51601 ccgaactcag caatccttgg agtaggtgct actattccga ctccaacagt ggttgatggt
    51661 gaaattgtag ctcgaccaat catggcaatg tgtttaacaa ttgaccaccg tattgttgat
    51721 ggaatgaatg gtgctaagtt tatggttgat cttaagaact taatggaaaa cccatttggt
    51781 ttacttattt gattccgtag gtcttaattt gatataataa gttattcgaa aaacgaaaga
    51841 ggaaataaaa aatggctttt gatgtaatta tgccaaaact tggggttgac atgcaagaag
    51901 gcgaaatcct tgagtggaaa aaaaatgaag gtgacaccgt taatgaaggt gatgtccttc
    51961 ttgaaataat gtctgataaa accaatatgg aaattgaagc agaagacact ggtgtacttt
    52021 taaaaattgt acatcaggca ggagatgttg ttcctgtcac tgaagtgatt gcctatatcg
    52081 gagaagaagg tgaagaagta ggtacgtcat caccttcggc tgatgcaact atcacagctg
    52141 aagatggtca atcagtttct ggacctgctg ctccttcaca agagacagtt gcagcagcaa
    52201 ctcctaaaga agaacttgca gctgatgagt acgatatcgt agtcgtaggt ggtggccctg
    52261 caggttatta cgcagcaatc cgcggtgctc aacttggagg aaaaattgcc attgttgaga
    52321 aaactgaatt tggcggtacg tgtttgaatg ttggttgtat ccctacaaaa acatatctta
    52381 aaaatgctga aatcctagat ggtttgaaag tagcagctgg acgtggtatc aaccttgctt
    52441 caactaacta tgcaattgat atggataaga ctgttgcctt taaaaattct gtagttaaga
    52501 cattgactgg tggtgtacgt ggtctcttga aagcgaacaa agttgaaatt ttcaatggac
    52561 ttggacaagt aaaccctgat aaatctgttg ttattggcga taaagttatc aaaggtcgta
    52621 acgtcgtcct tgcgactggt tctaaggtat ctcgtattaa tattccaggt attgaatcac
    52681 cattagtatt aacatcagat gatattcttg atttacgtga aattcctaag tcacttgctg
    52741 ttatgggtgg tggtgtcgta ggtattgagc ttggtcttgt atgggcttca tatggtgttg
    52801 acgtaactgt tattgaaatg gctgatcgca tcattccagc aatggataaa gaagtatcgc
    52861 tagaacttca aaaaatcctt gctaagaagg gaatgaagat taaaacatca gttggtgttt
    52921 ctgaaattgt tgaagccaat aatcaattaa cgcttaaatt aaacaacggc gaagaagttg
    52981 ttgctgataa agctcttctt tcaattggac gtgttccaca aatgaatggt cttgaaaatc
    53041 ttgagcctga acttgagatg gaacgtggac gtattaaagt taatgcttat caagaaactt
    53101 caattccagg tatctatgca ccaggtgatg ttaatggaac tcgtatgtta gctcatgctg
    53161 cataccgtat gggtgaagta gctgctgaaa acgcactcgg cggtaataag cgtaaagcac
    53221 atctagattt cactccagca gctgtttata ctcatcctga agtagcaatg gtggggatga
    53281 cagaagagca agcacgtgaa caatatggtg acatattagt tggtaaaaat agctttacag
    53341 gcaatggtcg tgctatcgct tcaaatgaag cacatggttt tgtaaaagta attgctgaac
    53401 ctaagtataa agagattctt ggtgttcaca ttattggtcc agctgcagcg gaattgatta
    53461 atgaagcttc tacgattatg gaaaatgagt tgactgtcta tgatgtagca caatctatcc
    53521 atggtcaccc aacattctct gaagttatgt acgaagcttt cttagatgtt ctcggtgaag
    53581 ctattcacaa ccctccaaaa cgcaaataat tttgtttaat atatttgaga ttgagctttt
    53641 atagcttcaa tctcttttca cgtatgtatt gtctgaatat tataaaatat tagttaggaa
    53701 tttaaaatga aatatatcgt taacacgtct aatgatccgg cttacaatgt tgctttagaa
    53761 gcttacgcct ttcaaaagtt gacagatatc gatgaaatct ttattctttg gattaatgaa
    53821 ccggctatca ttattggacg ccatcaaaat acaattcaag aaattaacaa agagttcatt
    53881 gataaaaatg gcatccatgt tgtccgtcgt ttgtcaggag gcggagcagt ttatcatgac
    53941 ttgaataatt taaactacac tattatttct aataatacac aagaaggtgc ttttgatttc
    54001 caaacttttt caaaaccagt tattgatact cttgctaaat taggtgttaa agcagaattt
    54061 actggtcgta atgatttaga aatcaatgga caaaaatttg caggaaatgc tcaagcttat
    54121 tacaaaggtc gtatgatgca tcatggttgt cttttatttg atgttgatat gtcagtctta
    54181 gggcaagcac tcaaagtatc taaagacaaa attgaatcta aagggataaa atctgtacgc
    54241 gcacgtgtta caaatattgt tgaccatctt tcagataaaa ttacagtaca agaatttagc
    54301 gatgccattt tagctcaagt gaaggaagaa tatccagaga tggatgaata tgttttgagt
    54361 gatgccgagt tatcagaaat ccaagctatg cgcgataatc aatttgctac atgggattgg
    54421 acgtatggta aagcacctga atacaccatt gaacgtggtg tccgctatcc agctggaaaa
    54481 attacgactt atgctaatgt tgaaaattca acgataaaat ctgtcaaaat ctttggtgac
    54541 ttctttggag tgaaacctgt agatgatatt gaaaaaatgt tggaaggtgt ccgctacgat
    54601 tacaaagatg ttttagcagc gcttaaaaca gttgatacaa gtcaatactt ctcacgtatg
    54661 acacccgaag agattacaaa agctattgta gactaaaatc aaaaaaccta gtcaaagtct
    54721 ttgcctttgg ctaggttttt tgtatggtta agtggc
//
LOCUS       DATRUK010000006        53485 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.006, whole genome shotgun sequence.
ACCESSION   DATRUK010000006 DATRUK010000000
VERSION     DATRUK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53485
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.006"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(47..907)
                     /locus_tag="VBE34_000287"
     CDS             complement(47..907)
                     /locus_tag="VBE34_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO7263040.1"
                     /translation="MTFKILTDSTSDLDEKWAQEHNVDIIGLTIELDGKTYETVGDEK
                     ITSDFLLERMQEGAKPTTSQINVGQFEEVFSTYAENDHALLYLALSSHLSGTYQSATI
                     AREMVLDKYPDAQIEIVDTMAASCGEGVLAMLATKERQEGKSLEEVKQKIESLLPKLN
                     TYFLVDDLNHLMRSGRLSKGAAIIGSVAKIKPLLKLDSEGKLVPFAKTRGRKKGIKEI
                     VTQATKTLSYSTLIIAYSGEKDSAQVMKEQLLADERIEEVIIRPLGPVISAHVGSGAL
                     ALFSLGEENR"
     gene            complement(1000..1518)
                     /locus_tag="VBE34_000288"
     CDS             complement(1000..1518)
                     /locus_tag="VBE34_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEO7263041.1"
                     /translation="MKKHSILLVDGYNMIAFWKDTRQLFKSNRLEEAREVLLRKLNHY
                     AHFEHIDIICVFDAQYVPGVRQRYDQYKISVIFTEEDETADSYIERAAAELNQSVLNL
                     VSVATSDLNEQWTIFSQGALRVSARELEQRVATVKSDLDKMSSQIDLSTPKLRPWNDE
                     QLGKLKDFLDGM"
     gene            complement(1515..2270)
                     /gene="rlmB"
                     /locus_tag="VBE34_000289"
     CDS             complement(1515..2270)
                     /gene="rlmB"
                     /locus_tag="VBE34_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEO7263042.1"
                     /translation="MTMKDKQFKEESSDLVYGLHAVTESLRANTGNKLYLQDDLRGKN
                     VDKVKALATEKKVSISWTPKKILSDMTNGGVHQGFVLKVSEFAYADLSEIMTKAENEE
                     NPLILILDGLTDPHNLGSILRTADATNVTGIIIPKHRSVGVTPVVSKTSTGAVEHVPI
                     ARVTNLSQTLDTLKDKDFWIFGTDMNGTPSHKWNTKGKLALVIGNEGKGISHNIKKQV
                     DEMITIPMNGHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(2373..2759)
                     /locus_tag="VBE34_000290"
     CDS             complement(2373..2759)
                     /locus_tag="VBE34_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEO7263043.1"
                     /translation="MIDVRLINGIALAFEGDAVYSLYIRRHLIMQGFTKPNQLHRKAT
                     QYVSANAQALLINAMLEENILTDEEQLIYKRGRNANSHTKAKNADIITYRMSTGFEAL
                     MGYLDMTGQIKRLETLIQWCIETIEK"
     gene            complement(2752..4095)
                     /locus_tag="VBE34_000291"
     CDS             complement(2752..4095)
                     /locus_tag="VBE34_000291"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEO7263044.1"
                     /translation="MIKIYDTMTRSLQDFIPLNEGKVNMYVCGPTVYNYIHIGNARSV
                     VAFDTIRRYFEYCGYQVNYISNFTDVDDKIIKGAAEAGMDTKSFSDKFISAFMEDVAA
                     LGVKPATKNPRVIDYMDEIIDFVKVLVDKEFAYEANGDVYFRVSKSHHYAKLANKTLE
                     DLEIGASGRVDGEGEIKENPLDFALWKSAKSGEVSWESPWGKGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVNVDNEKMSKSLGNF
                     ITVHDMLKSVDGQVIRFFLATQQYRKPVNFTEKAVHDAEVNLKYLKNTFNLPIQENAN
                     DEELEQFVKAFQEAMDDDFNTANGITVIFEMAKWINSGHYTSKVKETLAELLEIFGIV
                     FQEEVLDADIESLIEQRQEARANRDFATADRIRDELAKQGIKLLDTKDGVRWTRD"
     gene            complement(4092..4274)
                     /locus_tag="VBE34_000292"
     CDS             complement(4092..4274)
                     /locus_tag="VBE34_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263045.1"
                     /translation="MKDYVTTQILSILGILAGACLSYLKFAQGNSLLGIIWAAFALMY
                     IVRLYKAYQKQKKEKP"
     gene            complement(4284..4868)
                     /gene="cysE"
                     /locus_tag="VBE34_000293"
     CDS             complement(4284..4868)
                     /gene="cysE"
                     /locus_tag="VBE34_000293"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEO7263046.1"
                     /translation="MGWWKESIAIVKEQDPAARSSLEVILTYPGIKALAAHRLSHFLW
                     NHNFKLLARMHSQFWRFWTQIEIHPGATISEGVFIDHGSGLVIGETAIVEKGAMLYHG
                     VTLGGTGKDKGKRHPTIRKGALISAHSQIIGPIEVGENAKVGAAAVVLADVPADVTVV
                     GVPAKVVRVHGQKDDLQIRSIEHDREESYYSSKL"
     gene            complement(4877..5629)
                     /locus_tag="VBE34_000294"
     CDS             complement(4877..5629)
                     /locus_tag="VBE34_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEO7263047.1"
                     /translation="MTSTNELDIRLRAFINAPDNFLDSIGLVNALHHSTVWASKEPYA
                     IQVDGQEVVPVFTDITDLNHFKEEQESARDMFWESRRSLDVLDEAISHGLAGLVYNLK
                     KEGDFGNSTIFYCEDMVQFMNNYTTILNQLLNEDNIVADIMDKTYLVPAFVHPREEGS
                     FDRFFPTMSTPEGKSYVPVFSNLLSFEKWYNHNDFGGAFRKAQGVILAWTIDDIYKPR
                     NGENEIDDTFGVAINPFDEQQVLVDWSDVEND"
     gene            complement(5631..7760)
                     /gene="pnp"
                     /locus_tag="VBE34_000295"
     CDS             complement(5631..7760)
                     /gene="pnp"
                     /locus_tag="VBE34_000295"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEO7263048.1"
                     /translation="MSKQVFEMIFAGKKLVVETGQVAKQANGSVVVRYGDSTVLTAAV
                     MSKKMSTGDFFPLQVNYEEKMYAAGKFPGGFNKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSFDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVAYVDGNFI
                     INPTAQEQEASALELTVAGTKEAINMVESGAKELSEEIMLEALLKGHEAVCELIAFQE
                     EIVTAIGKEKAEVELLQVDPELQAEIIATHNIALQAAVQVEEKKAREAATEAVKEVVI
                     GEYEARYAEHEEYDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRRVDEIRPLDAVI
                     DFLPQVHGSGLFTRGQTQALSVLTLAPMGEAQIIDGLTPEYKKRFMHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIEGITPQILEEALAQAKKARFEILDVLHGAIAESRPQLAPTAPKIDMIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDEEGNVSIFSSDQAAIDRTKDIIASLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTANVADVLEIGEEVDVKVIKI
                     DDKGRVDASMKALLPRPPKADNPKKES"
     gene            complement(8140..8409)
                     /gene="rpsO"
                     /locus_tag="VBE34_000296"
     CDS             complement(8140..8409)
                     /gene="rpsO"
                     /locus_tag="VBE34_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEO7263049.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNDHIK
                     QHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            complement(8497..9756)
                     /locus_tag="VBE34_000297"
     CDS             complement(8497..9756)
                     /locus_tag="VBE34_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEO7263050.1"
                     /translation="MRAWKGIVLILSSIVVTLVAWQNAGLSEFVVPGLALTSLSLTFL
                     LSTKFRILESYFQGIENMYFYHKVMAVFSMILLLLHKIGLVQGGHGSEFAKTIGSAGL
                     YLFLSIVFVAYFGNFLKYEIWRFIHRFVYLVYILGLVHTFLILGDRILGNTLLSLIVL
                     GYAVIGVISGFYIIFLYSRMRFRRVGYVQKVTHLNHDTTEIEIAMKRPYRYDYGQFTF
                     LKIYQAGFESAAHPFSISGGHDRVIFLTVKASGDYTKSIYKQLKVGTKIALDRAYGHM
                     LFDKDKKEQVWIAGGIGITPFISFIRENSILTKRVDFFYTFSNQDNLIYQDMLESYAK
                     ANPNFKLHLNNSSLQGRLDFSQSVFEGQPTIFMCGPTSMTSTYAKVFRQKDAKSRLVY
                     EGFSFRDSWLSIFLLKTFDKVYSNLIK"
     gene            complement(9865..10794)
                     /locus_tag="VBE34_000298"
     CDS             complement(9865..10794)
                     /locus_tag="VBE34_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEO7263051.1"
                     /translation="MRCSTKEMRLVYRDFLLQANQENKQITVLEADLSSSMSTNVLAS
                     EFGKRYINLGIMEAEMVGLAAGLAIKGYKPYLHTFGPFASRRVFDQVFLSLGYSQLSA
                     TIIGSDAGVSAEMNGGTHMPFEELGLLRLIPKATIFEVSDDIQFEAILKQTLSIDGLK
                     YIRTIRKAPTAVYEGCEDFSKGFIQLRQGKDIALVASGIMVSRAIEAADYLKELGIEA
                     SVIDLFKIKPLPEELKPLLIDQSIVTIENHNRIGGIGSALCEWLSMEKDTTVSRMGID
                     ERFGQVGQMEYLLEEYGLAVKDIVEHCKSIYKS"
     gene            complement(10791..11648)
                     /locus_tag="VBE34_000299"
     CDS             complement(10791..11648)
                     /locus_tag="VBE34_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO7263052.1"
                     /translation="MTSPEERYSSLRRFATEIRLNTLETLNHLGFGHYGGSLSIVETL
                     AILYGDIMDINPEKFKESDRNYMVLSKGHAGPALYSTLYLKGFFDKTFLHSLNTNGTK
                     LPSHPDRNLTPGIDVTTGSLGQGISIATGIAYAQKIENSSYYTYTIVGDGELNEGQCW
                     EAIQFAAHHQLHHLIVFVDDNKKQLDGLTADICNPGDFVAKFEAFGFDAVRVKGDDIE
                     AIDKAIKTFQDSNSVKPKCIVLDSIKGQGVKELEELASNHHLRPDLQQKTMLERAVIS
                     LRESLEVVE"
     gene            complement(11651..13006)
                     /locus_tag="VBE34_000300"
     CDS             complement(11651..13006)
                     /locus_tag="VBE34_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEO7263053.1"
                     /translation="MKGLLDFLVNIARTPAILVALIAIIGLVLQKKGVPDIVKGGIKT
                     FVGFLVVSGGAGIVQNSLNPFGKMFEHAFHLVGVVPNNEAIVAVALTKYGSATALIML
                     AGMIFNILIARFTKFKYIFLTGHHTLYMACMIAVIFAVAGFTSFSLILFGSLALGIIM
                     SVSPAFVQKYMIQLTGNDKVALGHFGSLGYWLSGFIGGIVGDKSKSTEDIKFPKSLSF
                     LRDSTVSITISMAIIYLIVAVFAGEAYIAKEISNGVNGLVYALQLAGQFAAGVFVILA
                     GVRLILGEIVPAFKGISEKLVPNSKPALDCPIVYPYAPNAVLIGFISSFVGGLVSMIV
                     MIVTGTTVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFVQGILISFLPIFLMPVL
                     GGLGFKGSTFSDADFGLTGIILGALNHVGGAIAIVIGIVVILIGLFGISFVGKSTHKE
                     G"
     gene            complement(13019..13303)
                     /locus_tag="VBE34_000301"
     CDS             complement(13019..13303)
                     /locus_tag="VBE34_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7263054.1"
                     /translation="MLRIGTACGSGLGSSFMVQMNIESILKDLGVSDVEVEHYDLGGA
                     DPSAADVWIVGRDLEDSAGHLGDVRILNSIIDMDELRELVTGICQEKGLI"
     gene            complement(13306..15342)
                     /locus_tag="VBE34_000302"
     CDS             complement(13306..15342)
                     /locus_tag="VBE34_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEO7263055.1"
                     /translation="MVDNKTVVIMLVFLARKNLSLYELTVQTKFSIKVIIEQINYLNS
                     FLAKNHLTAIAHSAGRYQLLGDEKEHDKIVSLLEAEQFYLTQEERVCLIYLYSFCRRE
                     FVSNVHYQDFLKVSKNTTLSDIKMLRSKLAKRGISLTYTRAKGYSLVGDEMDKHQVAF
                     QMITQLLESPIGFWSLNYILSSWKFALSYEKLEKTVEYFYESFQLSPIQDRLEKSLYF
                     IILILCRYQRSVDRVLQGSPIVSEQLKELTTIIVTNLSQDISLSKPLDQKEKDYITLI
                     LSGCFEGEGTKDDDFFEALAKAIVDEMETISLLNFSNKEELLQGLKRHIIPAYFRLKY
                     GLTGDSGYTQNIKEHYSDLFLLVKKALRPLEEQVGLIPDSEISYFVIHFGGYLRQSGD
                     TQSMSYKALILCPNGVSSSLVIKEKLRGLFPQIHFHRVSKIEQLKLIDNQTYDMVFST
                     IFVETKKPNYLVSLMMTAEQVQQLKELVISDFPKACLDDFQLDQLIATIKKYAHVHCE
                     EELKLALRTMVKQDIFRKDVRPLLHQLITEETYQTSSEQMNWKEAIRLAAKPLLASGK
                     ITESYPEAMIEKVEEFGPFINLGKGIAIPHARPEDGVNSVGMSMLVLEQPIYLLDDPK
                     QEIYLLLCIAAIDNETHLKALSHLTTILRDNNNVKALLASRGYQDIEMIIKQED"
     gene            complement(15558..17183)
                     /gene="treC"
                     /locus_tag="VBE34_000303"
     CDS             complement(15558..17183)
                     /gene="treC"
                     /locus_tag="VBE34_000303"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEO7263056.1"
                     /translation="MTIDKRKVVYQIYPKSYKDTTGNGVGDLRGIIEKLPYLAELGID
                     MVWLNPFYPSPQRDNGYDISDYTAINPDFGTMDDFEEMIEVGRQYRIDFMLDMVLNHC
                     SIEHEWFKKALAGDRYYQDFFILRDNPTDWVSKFGGNAWSPFGDTGKYYLHLFDITQA
                     DLNWRNADVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEILENCPINDGKPAYTDRP
                     ITHDYLKMLNNASFGQADSFMTVGEMSSTTIANCILYTAPEREELSMAFNFHHLKVDY
                     KDGQKWTIMAFDFPALRDLFHSWGEGMSEGNGWNALFYNNHDQPRALNRFVDVKRFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDYSSMDDYVDIESLNAYQIMLDEGKSQE
                     EAFSIIRAKSRDNSRVPMQWDDSTNAGFSEGAPWLKVGKSYKEINVAKEKTGLIFTFY
                     QELIRLRKQLPIIADGNYKAAFKDNEKVYAFERHLDKEKLLVLNNFFAEKVKIKLPEN
                     YLQGQVLLSNYKDVTLDETVTLQPYQTLAILVS"
     gene            complement(17405..19435)
                     /gene="treP"
                     /locus_tag="VBE34_000304"
     CDS             complement(17405..19435)
                     /gene="treP"
                     /locus_tag="VBE34_000304"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEO7263057.1"
                     /translation="MEQFKHDAKALLEAIGGKENISAVTHCATRMRFVLNDSSKAKVK
                     VIEELPSVKGTFTNAGQFQVIIGNDVPIFYNAFVAVSGIEGVSKEAAKSAAQKNQNPL
                     QRVLTMLAEIFTPIIPAIIVGGLILGFRNILDAVPFEFLGQKVVDGVRQVDSSGHPIW
                     NTLVDVSTFWSGVDSFLWLPGEAIFHFLPVGIVWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYSVASTSAADIAKNWSWNFGYFTVQKIGYQAQVIPALLAGLSLSYLEIFWRKHI
                     PEVVSMIFVPFLSLVPAIILAHTVLGPIGWTLGKWISAIVLIGLTGPVKWLFGAIFGA
                     LYAPFVITGLHHMTNAIDTQLIADTKTHTTGLWPMIALSNIAQGSAVLAYYFMHRHDE
                     KEAQISLPAAISAYLGVTEPALFGVNVKYIYPFVAGMIGSSVAGLLATTFNVQANSIG
                     VGGLPGFLSINVKYMGYFFICMAVAIFIPLFLTLFFKKSGILTKTEEEKLVSDAVIAS
                     TTETKSAKEKAVVSGTKLSVVSPLSGLAKPLDQASDPVFSQGIMGKGVVIDPSDGELV
                     SPVDATVSVLFPTKHAIGLLTSEGVEFLIHIGMDTVNLEGKGFTSHVAQGDNVKVGDK
                     LITFDIPMIKEEGYIVETPILITNQEEFRSEELIDLPKQIKRGQALMVAKKI"
     gene            complement(19717..20343)
                     /locus_tag="VBE34_000305"
     CDS             complement(19717..20343)
                     /locus_tag="VBE34_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7263058.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEINLEVAPGQVLGIFGQSGCGKTS
                     LSKVLAGFLHPRSGEVLVDGSNLPNKAFRPVQLIQQHPEKTMNPLWPMKKSLEEAYYP
                     SRDLLDAFGIQEKWLNRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIVISHDFAMLEKLCNQCYMIEENRIVSFNGD"
     gene            complement(20327..21130)
                     /locus_tag="VBE34_000306"
     CDS             complement(20327..21130)
                     /locus_tag="VBE34_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7263059.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEIKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNASVTGDMIYRGQSLNSKRIKQLRGKDITLIPQSVN
                     YLDPSMKVKHQVRLGISENSKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDKVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGNGEQLQTEFARSLWRSLPQQEFLKGVTHDLRG"
     gene            complement(21142..21963)
                     /locus_tag="VBE34_000307"
     CDS             complement(21142..21963)
                     /locus_tag="VBE34_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7263060.1"
                     /translation="MILRRRTIVLWQLGIAISLILSILALNLYFHSTPLQTNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVILATVFGVLAGLGNSIID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYHLKN
                     KEFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(21960..22937)
                     /locus_tag="VBE34_000308"
     CDS             complement(21960..22937)
                     /locus_tag="VBE34_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7263061.1"
                     /translation="MKHITTVVIWKILRCLSLVFGVSILTFILLKQSPVDPVMASVNY
                     DTSLTPVQYKAIAHHYGLDKPAPVQYFIWLKNFIQGHLGTSLVYRQPVIDIIRSRAGA
                     SFILMGLSWVLSGLIGFILGTLSAFHQGKLLDRIVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLILPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETEWQIFKNHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(23050..24678)
                     /locus_tag="VBE34_000309"
     CDS             complement(23050..24678)
                     /locus_tag="VBE34_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7263062.1"
                     /translation="MTKYLKYISFVALFLASIFLVACQNQNSQTKERTRKQRPKDELV
                     VSMGAKLPHEFDPKDRYGIHNEGNITHSTLLKRSPELDIKGELAKKYKISKDGLTWSF
                     DLNDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELASKKVKGTRLLDIASNDVRGLSLPYVKK
                     GVVKNSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDRTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGNLKAEFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHYPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSPDLDKANKYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINVGKQGVHSHGHDWSLLTNIAEWTWDESAK"
     gene            complement(24921..25649)
                     /gene="lrgB"
                     /locus_tag="VBE34_000310"
     CDS             complement(24921..25649)
                     /gene="lrgB"
                     /locus_tag="VBE34_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HEO7263063.1"
                     /translation="MELLKTPIFGICFSLILYTIGQHLFKKSKGFFLLQPLFFAMVSG
                     IVILWLMSKGLGTDVKTFYTQAYKPGGDLIFWFLNPATIAFAVPLYKKNDVVKKYWVE
                     ILSSLVIGMIVSLMLIVAISKMVGLSQVGIASMLPQAATTAIALPITAAIGGNTAVTA
                     MACILNAVIIYALGKKLVSFFHLNDSKIGAGLGLGTSGHTVGAAFALELGELQGAMAA
                     IAVVVIGLVVDLVIPIFSHLIGLL"
     gene            complement(25651..26106)
                     /locus_tag="VBE34_000311"
     CDS             complement(25651..26106)
                     /locus_tag="VBE34_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO7263064.1"
                     /translation="MKHTSKETKTKEAPMFIQMSIYAAILLVSQMISMLLPKSLPIPT
                     TVIGLVLMYVLLTAKIIKVEWVDSFGALMISMIGFMFVPSGISVAANLDILKAEGLQL
                     VAVITISTVVMLVVVAYVARLILAIRELSVTDLFKKILPNHYSKKVEEK"
     gene            complement(26276..27016)
                     /locus_tag="VBE34_000312"
     CDS             complement(26276..27016)
                     /locus_tag="VBE34_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEO7263065.1"
                     /translation="MKILILDDEMFARQELSFLVEHSQEVDNPEIFQAEDISEAEKIL
                     FRQQIDLIFLDISLSEENGFTLANQLSQLAHPPLVVFATAYDNYAVKAFESNAVDYIM
                     KPFEQQRVDMALSKVKKLSQLTTASDVEQAIPKKASAELLTLTLSDRSVVVKMQDIVA
                     ASVEDGELTVSTVQKTYTIRKTLNWFKSRAVAPYFLQIHRNTVINLEMIEEIQPWFNH
                     TLLLIMSNGEKFPVGRSYLKDLNEHLTM"
     gene            complement(26997..28742)
                     /locus_tag="VBE34_000313"
     CDS             complement(26997..28742)
                     /locus_tag="VBE34_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEO7263066.1"
                     /translation="MLMVLLFQRLGIIMILAFLLVNNSYFRQLIEERSKRETVVLVII
                     FGLFVIISNITGIEIKGDRSLVERPFLTTISHSDSLANTRTLVITTASLVGGPLVGSI
                     VGFIGGVHRFFQGSFSGSFYIVSSVLVGIVSGKIGDKLKENHLYPSTSQVILISIIAE
                     SIQMLFVGIFTGWELVKMIVIPMMILNSLGSTLFLAILKTYLSNESQLRAVQTRDVLE
                     LTRQTLPYLRQGLTPQSARSVCEIIKRHTNFDAVGLTDRSNVLAHIGVGHDHHIAGQP
                     VKTDLSKSVIFDGEPRIAQDKAAISCPDHNCQLNSAIVVPLKINDKTVCALKMYFAGD
                     KTMSEVEENLVLGLAQIFSGQLAMGITEEQNKLASMAEIKALQAQINPHFFFNAINTI
                     SALIRIDSDKARYALMQLSTFFRTSLQGGQDREVTLEQEKSHVDAYMNVEKLRFPDKY
                     QLSYDISAPEKMKLPPFGLQVLVENAVRHAFKERKTDNHILVQIKPDGHYYCVSVSDN
                     GQGISDTIIDKLGQETVAESKGTGTALVNLNNRLNLLYGSVSCLHFSSDKNGTKVWYR
                     IPNRIREDEHENFNS"
     gene            complement(28769..29413)
                     /locus_tag="VBE34_000314"
     CDS             complement(28769..29413)
                     /locus_tag="VBE34_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7263067.1"
                     /translation="MEKVIIFDMDGVIVDSEYTFLDNKTEMLREEGIDTDVSYQYQYM
                     GTTFEFMWQAMKEEFGLPKTVKEYIAEMNRRRQAIVTRDGVRPIKGAQQLIHWLHQHG
                     YRLAVASSSPMVDIKRNLKELGVTECFEYMVTGEDVSSSKPAPDVFLRAAELLDVDPK
                     VCIVIEDTRNGSLAAKAAGMYCFGFANPDYPPQDLSMADKVISDYQDIYIYLPE"
     gene            complement(29537..29932)
                     /locus_tag="VBE34_000315"
     CDS             complement(29537..29932)
                     /locus_tag="VBE34_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO7263068.1"
                     /translation="MYNKVIMIGRLTAKPEMVKTPTDKSVTRATVAVNRRFKGSNGER
                     EADFINVVMWGRLAETLASYGTKGSLISIDGELRTRKYEKDGQTHYITEVLASSFQLL
                     ESRAQRAMRENNVSGDLSDLVLEEEELPF"
     gene            30013..30729
                     /locus_tag="VBE34_000316"
     CDS             30013..30729
                     /locus_tag="VBE34_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO7263069.1"
                     /translation="MTNVTSYKEMLAKPWGKIQYEITFAQLSHIKNQNVLDFGAGFCL
                     TAQHLAKENNVTAIEPNPKLLYDNQSDNIYKILGSYEALRALPDQSFDTIICHNVLEY
                     IDKHNHPAYFDEFSRLLKPNGELSLIKHNIIGKILQSVIFSNDTSTAMELLTGEANFK
                     SASFDQGNIYTLEELKQNTNLLVERYQGIRTFYSLQPNHFKTETDWLNKMLAIELSVA
                     DKAPYKDIAFLQHITLKKSL"
     gene            complement(30783..31409)
                     /locus_tag="VBE34_000317"
     CDS             complement(30783..31409)
                     /locus_tag="VBE34_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEO7263070.1"
                     /translation="MIFTYNREHVGDTLMVIVKDSQGAKLDVDRRGQVARVYLQDSKE
                     TVAWNIFEVSSLIAIEGVGQVTLSDQVIKILNAELLKEGFEDSLVNNIEPTFVVAQIK
                     EMIDHPDSDHLHICQVEINDGKTVQIVCGAPNASVGLKTVAALPGAMMPNGSLIFPGK
                     LRGEDSFGMLCSARELALPNAPQVRGIIELSDQVIVGESFDANKHWKN"
     gene            complement(31442..31765)
                     /locus_tag="VBE34_000318"
     CDS             complement(31442..31765)
                     /locus_tag="VBE34_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEO7263071.1"
                     /translation="MILPESYEEIAAYIDSTKKVVFFFTADWCPDCQFIYPVMPSIEK
                     DFSDFVFVRVNRDDYIELAQQWNIFGIPSFVVVENGQELGRLVNKNRKTKAEITKFLA
                     EINHK"
     gene            complement(31762..32046)
                     /locus_tag="VBE34_000319"
     CDS             complement(31762..32046)
                     /locus_tag="VBE34_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEO7263072.1"
                     /translation="MKNKKILFGTGLAGVGLLAAAGYTLTKKVTDYKRQQITQTLREL
                     FSQMGDIQVFYFNEFESDIKMTSGGLVLEDGRIFEFIYRQGVLDYVEVSK"
     gene            32207..32446
                     /locus_tag="VBE34_000320"
     CDS             32207..32446
                     /locus_tag="VBE34_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263073.1"
                     /translation="MKEKLLVPALNCLLGLLFLGDHWFLFRLQNGELFAIVTLILLCT
                     ASFLINKNHPKNLTLIAMVITFLAVLSFLSTFLLA"
     gene            32631..33698
                     /gene="pepA"
                     /locus_tag="VBE34_000321"
     CDS             32631..33698
                     /gene="pepA"
                     /locus_tag="VBE34_000321"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEO7263074.1"
                     /translation="MSDLFNKIKTVTELDGIAGYEHNIRNFLRQEITPLVDQVETDGL
                     GGIFGVKNTHETNAPKVMVAAHMDEVGFMVSHIQPDGTFRVLEVGGWNPLVVSSQRFT
                     LYTRSGDAIPVISGSVPPHFLRGQSGGTTLPKISDIVFDGGFTDKNEAESFGIAPGDI
                     IVPKSETILTANQKHIMSKAWDNRYGVLMVTELLKSLKDQSLSNTLIAGANVQEEVGL
                     RGAHVSTTKFNPDIFLAVDCSPAGDIYGEQGKIGEGTLIRFYDPGHIMLKDMRDFLLT
                     TAEEAGIKYQYYAAKGGTDAGAAHLKNSGIPSTTIGVCARYIHSHQTLYAMDDFLQAQ
                     AYLQAIVNKLDRSTVDIIKGY"
     gene            33768..34538
                     /locus_tag="VBE34_000322"
     CDS             33768..34538
                     /locus_tag="VBE34_000322"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEO7263075.1"
                     /translation="MKIGIIGVGKMASAIIQGLKQTQHDIVISGSCLERSKEIAERLD
                     VTYAESHQSLINQADIIMLGIKPQLFEKVLLPLDITKPIISMAAGISLARLSQLTRSD
                     LPLIRIMPNINAQILQSCTAICYNNHVSDELRQLAKEITDSFGSSFDIAEINFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKYGFPKEQALSIVGQTVLASSQNLLQGQNSTSDLIDN
                     ICSPGGTTIAGLLDLEKNGLTHSVISAIDATIEKAKKL"
     gene            complement(34559..35224)
                     /locus_tag="VBE34_000323"
     CDS             complement(34559..35224)
                     /locus_tag="VBE34_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7263076.1"
                     /translation="MSFIIKKGKILALLIAFLVINQLVPILAVWLLKNHYQTPFTSIL
                     LIGLELLIIALFLYYAKVKQIIRWKVLLTRKALVTILLGWLSLRVPQIIGYLIMTMQG
                     VKDTANQTVIMELTKQLPLALMLIFAIIGAPIMEEIIFRYIIPKELFAKHQKWGFVIG
                     TLAFALIHSPSDIGSFIIYAGMGAILSFVYYKTEHLEYSIMIHFINNALAYSVLISTM
                     MNN"
     gene            complement(35293..35748)
                     /locus_tag="VBE34_000324"
     CDS             complement(35293..35748)
                     /locus_tag="VBE34_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263077.1"
                     /translation="MLIKGLLSPNSKVSPKDYKVYMRFLSFVFSVCSLIMIVSSFLHT
                     FESNFTKGFSFGSGIALFAGSFYFLVITFSEKRFQHYYTKVFDERNQFIANMSCRLLF
                     LLSVLVLVVLCLINVWFGITFSYAKFLLLILSFVLYGYLLIYFILQKLL"
     gene            complement(35789..35926)
                     /locus_tag="VBE34_000325"
     CDS             complement(35789..35926)
                     /locus_tag="VBE34_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263078.1"
                     /translation="MSRMWTIDFCPPIVYQQYVFISTISISIAMSIFAIYRYASLKKT
                     I"
     gene            complement(35985..36191)
                     /locus_tag="VBE34_000326"
     CDS             complement(35985..36191)
                     /locus_tag="VBE34_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7263079.1"
                     /translation="MKNSLQKLRKSRKLSQAELAVALGVTRQTIISLEKEKYTASLEL
                     AFKIARYFDKQIEEVFIYTESEGG"
     gene            complement(36342..37535)
                     /locus_tag="VBE34_000327"
     CDS             complement(36342..37535)
                     /locus_tag="VBE34_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEO7263080.1"
                     /translation="MSKTIAINAGSSSLKWQLYEMPEEKVVAKGIIERIGLKDSISTV
                     KFDDKKDEQILDIVDHTQAVKILLEDLTKHGIIKDFNEITGVGHRVVAGGEYFKESAL
                     VDDKVVEQVEELSALAPLHNPAAAAGIRAFREILPDITSVCVFDTAFHTTMQPHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHQYVAQEAAKQLGRPLEELKLITAHVGNGVSITANYH
                     GQSIDTSMGFTPLAGPMMGTRSGDIDPAIIPYLVANDPELEDAAAVVNMLNKQSGLLG
                     VSGTSSDMRDIEAGLQSKDPNAVLAYNVFIDRIKKFIGQYLAVLNGADAIIFTAGMGE
                     NAPLMRQDVIAGLSWFGIELDPEKNVFGYFGDITKPDSKVKVLVIPTDEELMIARDVE
                     RLKAK"
     gene            complement(37567..38541)
                     /locus_tag="VBE34_000328"
     CDS             complement(37567..38541)
                     /locus_tag="VBE34_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7263081.1"
                     /translation="MNFEKIETAYELILENIQTIENQLKTHIYDALIEQNSYYLGSSC
                     DLDIVVVNNQKLRQLDLSQEEWRRTFQFIFIKSAQTEQLQANHQFTPDSIGFILLFLL
                     EELTSQETVDVLEIGSGTGNLAQTLLNNSSKELNYMGIEVDDLLIDLSASIAEIIGSS
                     AQFIQEDAVRPQILKESDVIISDLPVGYYPNDDIAKRYAVSSSKEHTYAHHLLMEQSL
                     KYLKKDGIAIFLAPENLLTSPQSDLLKEWLKGYADVIAVLTLPETIFGSRQNAKSIFV
                     LKKQAEQKPETFVYPLTDLQNRENMANFIENFQKWSRENSHYSKNMIE"
     gene            complement(38656..38879)
                     /locus_tag="VBE34_000329"
                     /pseudo
     CDS             complement(38656..38879)
                     /locus_tag="VBE34_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(38857..39249)
                     /locus_tag="VBE34_000330"
     CDS             complement(38857..39249)
                     /locus_tag="VBE34_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEO7263082.1"
                     /translation="MVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLN
                     AEFEGAHLEYLRQNKLYLRKQDKIVTFGKSNKDDFRKTGYNGRGYQPMVYGLDNCQMS
                     QTKSMVKLVFYFKDGLKRTFYYDFKEET"
     gene            complement(39238..39313)
                     /locus_tag="VBE34_000331"
                     /pseudo
     CDS             complement(39238..39313)
                     /locus_tag="VBE34_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793382.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
     gene            complement(39310..40401)
                     /locus_tag="VBE34_000332"
     CDS             complement(39310..40401)
                     /locus_tag="VBE34_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEO7263083.1"
                     /translation="MTLRQVTLKNTHQTSGIDKWISWLKKDISVRNRHKSKKLSLKKQ
                     RKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDRMNKALLEGKDLSKMLGELGFS
                     DTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGL
                     RHYLMPQLGENNFATRLITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTI
                     PLVGSYVKLYLTAYYAREWGNLLSQGVELDQIVKVMQNQKSKLFREIGYDMEEGFLSG
                     KAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQP
                     VIFIFVALIIVMIYAAMLLPMYQNMEILS"
     gene            complement(40286..41218)
                     /gene="tadA"
                     /locus_tag="VBE34_000333"
                     /pseudo
     CDS             complement(40286..41218)
                     /gene="tadA"
                     /locus_tag="VBE34_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            complement(41390..41755)
                     /locus_tag="VBE34_000334"
     CDS             complement(41390..41755)
                     /locus_tag="VBE34_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEO7263084.1"
                     /translation="MYQVVKMFGDWEPWWFIEGWEEDITEIAEYDTLSEALLYFQEEW
                     DRGQEKWPYFQSKSSLLATFWSIKEKRWCEECDEYLQQYHSLMLLKEWQEIPKEESIE
                     RFEVFNKIAELPSACSLNL"
     gene            complement(41869..45519)
                     /gene="rpoC"
                     /locus_tag="VBE34_000335"
     CDS             complement(41869..45519)
                     /gene="rpoC"
                     /locus_tag="VBE34_000335"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEO7263085.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIICDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPMDTPLEPKSLLTEREYREKLQ
                     EYGYGSFVAKMGAEAIQDLLKRVDLDAEIAVLKEELKSATGQKRVKAVRRLDVLDAFK
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDLAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDHDEAVMAYQNGYVHLHTRVGIAVDSMPNKPWTEEQKHKIMVTTVGKI
                     LFNDIMPEDLPYLIEPNNANLTEKTPDKYFLEPGQDIQAVIDNLEINIPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     DINKAFRRGLMTEEDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLTITAITDGKEVTETLEERLIGRYTKKSIKHP
                     ETGEILVGADTLITEDMAAKVVKAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGEVVAIEEDSSTRTKKVFVKGQTGEGEYVVPFTARMKVEVGDEVARGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPLAVNEVEIIEGTPVDA
                     EVTEVSTPTTED"
     gene            complement(45636..49211)
                     /gene="rpoB"
                     /locus_tag="VBE34_000336"
     CDS             complement(45636..49211)
                     /gene="rpoB"
                     /locus_tag="VBE34_000336"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO7263086.1"
                     /translation="MAGHEVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDAG
                     LKEVFEDVLPISNFTDTMDLEFVGYELKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDAKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGDSELVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNLKTRLLNQTIAENLVDGETGEILVEAGTVMTRDVIDSIAEHIDGDLNKF
                     VYTPNDYAVVTEPVILQKFKVVAPTDPDRVVTIVGNSNPEDKVRALTPADILAEMSYF
                     LNLAEGIGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRSTGAVTNEIV
                     WLTADEEDEFTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFPSSIVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAKH
                     DGRVIFSDAEKVEVRREDGSLDVYHVQKFRRSNSGTAYNQRTLVKVGDLVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEDSLRDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGVVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWETVQEAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDVMHVDDLEKARVKQEAEEKQAEQVSEVVQED"
     gene            complement(49735..52032)
                     /locus_tag="VBE34_000337"
     CDS             complement(49735..52032)
                     /locus_tag="VBE34_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7263087.1"
                     /translation="MFKGNKKLNSSKLGDYTPLEFGSIFLRIVKLLSDFIYVIILLFV
                     MLGVGLAVGYLASQVDSVKVPSKNSLVTQVNTLTRVSRLTYSDKSQISEIATDLQRTP
                     VAKDAISDNIKKAIIATEDENFNDHKGVVPKAVLRAAAGSVLGFGESSGGSTLTQQLL
                     KQQILGDDPSFKRKSKEIIYALALERYMDKDSILSDYLNVSPFGRNNKGQNIAGIEEA
                     AQGIFGVSAKDLTIPQAAFLAGLPQSPIVYSPYTADAQLKSDKDLSFGIKRQKNVLYN
                     MYRTRALTKDEYKSYKDYDIKKDFIKPAVATTNHHDYLYYSALSEAQKVMYNYLIKKD
                     NVSEHDLKNDETRATYRHRAIEEIQQGGYTIKTTINKSVYQAMQDAAAQYGGLLDDGT
                     GKVQMGNVLTDNSSGAIIGFIGGRNYSENQNNHAFDTARSPGSSIKPILPYGIAIDQG
                     MLGSGSVLSNYPTTYSSGEKIMHADEEGTAMVNLQESLDISWNIPAFWTYKMLRDRGV
                     DVKNYMEKLDYPIENFGIESLPLGGGIDTSVAQQTNLYQMIANGGVYHKQYMIESIED
                     SNGKVIYNHESKPVRVFSKATATILQQLLHGPINSGKTTTFKNRLQGLNSGLAGVDWI
                     GKTGTTNSTSDVWLMLSTPKVTLGGWAGHDNNASLAKLTGYNNNANYMAHLVNAINNA
                     DGNTFGKSERFRLDDSVIKAKVLKSTGLQPGVVTVNGRRITVGGESTTSYWAKNGPGT
                     MTYRFAIGGTDSDYQKAWSTLGGKR"
     gene            52143..53402
                     /locus_tag="VBE34_000338"
     CDS             52143..53402
                     /locus_tag="VBE34_000338"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEO7263088.1"
                     /translation="MNIFDELKERGLVFQTTDEDALRKALEEGSVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDVERSLQTKKTVVSWGNKIR
                     GQLSNFLEFETGDNKAVLVNNYDWFSNISFIDFLRDVGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFYELNKNYNVTLQIGGSDQWGNMTAGTELIRRKSNGVSHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDADAVRFLKIFTFLSLKE
                     IEDIRIQFEEAPHQRLAQKTLAREVVTLVHGEKAYKEAVNITEQLFAGNIKGLSVKEL
                     KQGLRGVPNYHVQTEDNLNIIDLLVTSGVVNSKRQAREDVSNGAIYINGDRIQDLEYT
                     ISENDKLENEITVIRRGKKKYFVLNFK"
BASE COUNT        17102 a        10924 c         8567 g        16892 t
ORIGIN      
        1 aatgtatact aaaagaacat aacgtactgt tatgcccttt gatttgttat cggttttctt
       61 cccctaaaga gaatagagct aaagcacctg agcccacatg agctgaaata actggcccta
      121 atggacgaat aataacttct tcaatacgct catctgctaa taactgttct ttcataactt
      181 gagctgaatc tttctctcca ctataagcaa taattaaagt gctataagat agtgtttttg
      241 ttgcctgagt cacaatctct ttaatacctt ttttacggcc ccttgtcttg gcaaatggta
      301 ctaatttacc ctcactatct aacttaagta atggtttaat cttagcaaca cttccaatga
      361 tagctgcacc ttttgataag cgaccactac gcattaaatg gttgagatcg tcaactaaaa
      421 agtatgtgtt taatttaggg agaagtgatt caattttttg tttcacttct tctaaagact
      481 taccttcttg tcgctcttta gttgctaaca tcgctaagac accctcacca catgaagctg
      541 ccattgtatc cacaatctca atttgagcat caggatattt gtctaagacc atctcacgcg
      601 caatagttgc actctggtaa gtccctgata aatgtgaaga tagcgctaaa tataacaagg
      661 catggtcatt ttctgcatag gttgaaaaaa cttcttcaaa ctgaccaacg ttaatttggc
      721 ttgttgtagg ctttgctccc tcttgcatac gctccaacaa gaagtcacta gtaatttttt
      781 cgtctccgac tgtctcataa gtctttccat caagttcaat ggttaaacct ataatatcga
      841 cattgtgctc ttgagcccac ttttcatcta aatccgatgt cgaatccgtt agtattttaa
      901 aagtcattgt tacctcaatc tattattttt atttgataat caaagtagtt ccaatattat
      961 atcaaaaaaa ttgttggctg gctaataaag tgttagtttt tacataccat ctaaaaaatc
     1021 cttgagtttt cctagttgtt catcattcca cggccttaac tttggcgtac tcaaatcgat
     1081 ttgactggac atcttatcca aatctgattt tactgtcgct acacgctgtt ccaattctct
     1141 agctgagaca cgtaaagcac cctgagaaaa tatagtccat tgttcattta aatcactggt
     1201 agcaacggag actaaattta acacagattg attaagttct gccgctgctc gttcaatata
     1261 gctatccgca gtttcgtcct cctcagtaaa gataacggat attttgtatt ggtcataacg
     1321 ttgacgtaca ccaggaacat actgggcatc aaatacacaa ataatatcaa tgtgttcgaa
     1381 atgtgcgtaa tgatttagct ttctaagtaa tacttccctt gcctcttcta aacgattgct
     1441 tttaaaaagc tgtcgagtat ctttccaaaa agcaatcata ttgtagccat caacgagtaa
     1501 aatagaatgt tttttcatag gcggtttcta aatacttcat acatcaaaat agcagctgcg
     1561 acactggcat ttaaactctg aacatgacca ttcatgggaa ttgtaatcat ttcatctact
     1621 tgctttttga tattatgaga aatacctttt ccttcattac caataactag ggctaattta
     1681 cctttggtat tccacttatg cgatggtgtc ccattcatat cagtgccaaa tatccagaag
     1741 tccttatctt ttaaagtatc caaagtttga ctgagattag ttacccttgc gataggaaca
     1801 tgttcaacag ctcctgtaga tgtctttgaa accaccgggg tcactccaac agaacgatgt
     1861 ttggggatga taatgcctgt tacattggtt gcatcagctg tgcgtagaat agaacctagg
     1921 ttatgagggt cggtcagacc atcaaggatt aaaattagag gattttcttc attctctgcc
     1981 tttgtcatta tctctgataa atccgcatag gcaaattccg aaacttttag aacgaatcct
     2041 tggtgaactc caccatttgt catgtctgat aagatttttt taggagtcca ggaaatggat
     2101 actttttttt cagtcgctag tgctttgact ttatcaacgt tctttcctct taagtcatct
     2161 tgcaagtata atttattacc agtattagct cttaaactct ctgtcacagc atgtaagcca
     2221 tagacaaggt cacttgattc ttctttaaat tgtttatctt tcatagtcat attatatcat
     2281 gaatgctata gcataaagct ttttatagca atattttacc aacaaatcct aatgtctcta
     2341 taaaaaagag aggatatagc ctctcttttt gtttattttt caatggtttc aatacaccac
     2401 tgtattagag tttccaagcg ctttatctga ccagtcatat ccaagtaccc cataagagcc
     2461 tcaaaacctg tagacatacg ataggtaata atatcagcat ttttagcttt tgtatgacta
     2521 ttcgcattac gtcctcgttt ataaattaac tgctcttcat cagttaaaat attttcttct
     2581 aacatggcat taattaacaa tgcttgggca ttagcagaaa catattgggt tgctttccga
     2641 tgtaactgat taggtttggt aaaaccctgc ataattaaat ggcgtcggat atacaaagaa
     2701 taaactgcat ctccttcaaa agctaaagca ataccattaa tcaaacgaac atcaatcacg
     2761 agtccacctc acgccatcct tagtatctaa caatttaatt ccttgttttg ctaattcatc
     2821 tctaatacga tcagcagttg caaaatcacg attagctcgt gcttcttgac gttgttctat
     2881 taaagattca atatcagcat caagaacttc ctcttggaat acaataccaa aaatttctaa
     2941 taattcagca agggtttcct taacttttga agtatagtgt cctgaattaa tccattttgc
     3001 catttcaaag ataacagtaa taccgtttgc tgtattaaaa tcatcatcca tcgcttcttg
     3061 aaatgctttc acaaattgtt ctaattcttc atcattagca ttctcttgga ttggtaagtt
     3121 gaaagtattt tttaaatatt taaggttaac ttccgcatca tgaacggcct tttcagtgaa
     3181 attcacgggt ttacgatact gttgtgtcgc taagaaaaaa cgtattactt gtccgtcaac
     3241 gctcttcaac atatcgtgaa cagtaataaa gttaccaaga gatttagaca ttttctcgtt
     3301 atcaacgtta acaaaaccat tatgcatcca gtaatttgca aaggtcttac ctgttttagc
     3361 ttcagattgt gcaatctcat tcgtgtgatg tggaaattct aaatcagctc ctccaccatg
     3421 gatatcaata gtatcaccaa ggatctcagt tgccataaca gaacattcaa tatgccaccc
     3481 tgggcgccct ttcccccaag gactttccca tgagacttcc ccagatttag ctgatttcca
     3541 aagtgcaaaa tctaaaggat tctctttaat ctctccttcc ccatcaacac gaccacttgc
     3601 accaatctct aaatcttcca aagttttatt ggctaatttt gcataatgat gtgatttact
     3661 tactctaaaa taaacatctc cattagcttc ataggcaaat tccttatcaa ctaaaacttt
     3721 aacaaaatca ataatctcat ccatatagtc aataactcga ggattttttg tagcaggttt
     3781 gacacctaat gcagcaacat cttccataaa tgctgaaata aacttatctg aaaaggattt
     3841 tgtatccata cctgcttcag ctgcaccttt tataatttta tcatcaacat cagtaaagtt
     3901 agaaatataa ttgacttggt aaccacaata ctcaaaataa cgacgaatag tatcaaaagc
     3961 tactacactg cgggcattcc cgatgtgtat atagttataa actgttggcc cacagacgta
     4021 catgttgact ttaccttcat tgagaggtat aaaatcttgt aaactgcgag tcatagtgtc
     4081 gtaaattttt atcatggttt ttcctttttc tgtttttgat aagctttata caaacgaaca
     4141 atatacatta aagcaaaggc agcccaaata attcctaaga gagagttacc ttgtgcaaac
     4201 tttaaatagc ttaaacaagc tcctgctagt atacctaaaa ttgataatat ttgagttgtt
     4261 acataatcct tcacaaatac ctcctaaagt ttggacgaat aataactttc ttctcgatca
     4321 tgttcaatac ttcggatttg aagatcatcc ttttgaccat ggacacggac aactttagct
     4381 ggaacaccaa ctactgttac gtctgctggc acatctgcaa ggacaactgc tgcagcaccg
     4441 actttagcat tttctccaac ctcaattgga ccgatgattt gagaatgtgc tgaaattagc
     4501 gcacctttac gtatggttgg gtgtcgtttg cctttgtctt tacctgtgcc acccaatgta
     4561 accccatggt atagcatagc acctttctct acaatggcag tttctccaat gaccaatcct
     4621 gaaccgtggt caataaaaac cccttccgaa atcgtcgctc cgggatgaat ttcaatttgt
     4681 gtccagaaac gccaaaattg actgtgcata cgtgctaaca atttaaaatt atgattccat
     4741 aaaaaatgag atagcctatg agctgcaaga gcttttattc ctggataagt taaaataact
     4801 tctagagaac tacgagcagc tggatcttgt tctttgacaa tagctatact ttctttccac
     4861 caccccatag ttttcctcaa tcgttttcta catcagacca atcaactaaa acttgttgtt
     4921 catcaaatgg attgatggct actccaaagg tatcatcaat ctcattttct ccattacgcg
     4981 gtttataaat atcgtcaatc gtccaggcta aaattactcc ttgtgctttc ctaaaagcac
     5041 caccaaaatc attatggtta taccattttt caaaacttaa aaggtttgag aatactggaa
     5101 cataactttt tccttcgggg gtagacattg tagggaaaaa acggtcaaaa ctaccttctt
     5161 cacgtggatg gacaaaggca ggtaccaagt aggttttatc catgatatcg gcaacaatgt
     5221 tatcttcatt taacaactga ttcaagattg tggtgtaatt gttcataaat tgcaccatat
     5281 cttcacagta gaaaatagtc gagttcccaa aatcgccttc tttttttaaa ttatagacca
     5341 atcctgctaa gccatgtgaa atagcttcat caagaacgtc taaactacga cgactctccc
     5401 aaaacatatc tcgagcactt tcttgctcct ctttgaaatg attaaggtca gtaatatccg
     5461 taaaaacagg aacaacttct tgaccatcta cttgaatagc atagggttcc ttgcttgccc
     5521 aaaccgtaga gtggtgcaag gcattcacaa gacctatact atctaaaaaa ttatccggtg
     5581 cattgataaa agcacgcaat ctaatgtcta actcgtttgt acttgtcata ttaagattcc
     5641 tttttaggat tgtcagcttt tggaggacgt ggtaataatg ctttcataga ggcatcaact
     5701 cgccctttat catcaatttt gatgactttc acatcaactt cttcaccaat ttcaagaaca
     5761 tcagctacat ttgctgtacg tgtccatgca atttctgaaa tatgtaccaa tgcatctgtt
     5821 ttatcaaaga ggttgacaaa ggcaccgaat ttctcgatac gaactacttt agcatggtaa
     5881 acttctccca cttttgcttc acgtactaaa cttgcaataa tgtctttcgt acgatcaata
     5941 gcagcctgat cactagagaa gatagataca tttccttctt catcaatatc aatcttaaca
     6001 cctgtttcag cgatgatttt atcgatagtt tcgccgcctt taccgatgac aactttaatc
     6061 ttatcaacat cgatttttat catatcaatc ttaggtgccg ttggtgctaa ttgtggacgt
     6121 gactctgcga tagctccatg aaggacatca agaatttcaa agcgtgcttt tttagcttgc
     6181 gcaagagctt cttccaaaat ttgaggggta ataccttcaa ttttaatatc catttgaagt
     6241 gctgtaatcc cttcacgtgt acccgcaacc ttaaagtcca tatcgccaaa atggtcttct
     6301 aatccttgaa tatcagtaag gactgtgtag tttgttccat ctgatatgag tcccatggca
     6361 ataccagcaa ccggagcttt tataggcaca ccacctgcca taagagcaag cgttccagca
     6421 catatagaag cctgagatga tgaaccgttt gattctaaaa cttctgcaac tagtcggata
     6481 gcatacggaa actcctccaa gcttggaagg acttgttcta atgcacgctc tcctaaagca
     6541 ccgtgaccaa tttcacgacg ccctgcagct ccataacgtc ccgtctcacc aactgaatat
     6601 tgtgggaagt tatagtggtg cataaagcgt ttcttatact ctggtgttag cccatcaatg
     6661 atttgtgctt cacccatagg tgctaacgtt aatactgata atgcttgggt ttgtcctcgt
     6721 gtaaagagac ctgatccatg aacttgtggt aaaaaatcaa tcactgcatc taatggacga
     6781 atctcatcca cacgacgtcc atcaggacga accttatctt ctgtaatgag gcgacgaact
     6841 tctgcatgtt ccatttgttc taaaatttca gcaacatcac gcataatgcg atcatattct
     6901 tcatgttcag cgtaacgcgc ctcatattca cctataacta cttctttaac tgcttctgta
     6961 gctgcttctc tagccttttt ctcttcaact tgtactgcag cctgtaaagc tatgttatga
     7021 gtagcaataa tttctgcttg aagttctgga tcaacttgaa gaagttctac ttcggctttt
     7081 tctttaccaa tagcagttac aatctcttct tgaaaggcga ttaattcaca gacagcctca
     7141 tgtcctttaa gaagagcctc tagcatgatt tcttctgaaa gttctttcgc tccagattca
     7201 accatgttaa tcgcttcttt agtacctgca accgttaatt ctaacgcaga agcctcttgt
     7261 tcttgtgctg tcggattaat aataaaattg ccatcaacat aagcaacttg aactccagca
     7321 attggcccat tgaaaggaat atcggaaatc gataatgcta atgatgaacc aaacatagca
     7381 gccattggtg cgctagcatt ttcatcaaat gaaagaacag tattgatgac ctggacttcg
     7441 ttacgaaatc cctcagcaaa catcggacgg attggtctat caataagacg tgctgttaag
     7501 gtagcatccg tacttggacg cccttcacgt ttgttaaaac cacccggaaa tttcccagca
     7561 gcatacattt tttcttcgta attgacttga agtgggaaaa aatcacctgt tgacattttc
     7621 ttagacataa ctgctgctgt aagtacagta gaatctccat aacgaacgac aactgatcca
     7681 tttgcttgtt tagccacttg gccagtttca acaactaatt ttttaccagc aaaaatcatt
     7741 tcaaagactt gttttgacat attattctcc atattttttg tttacgcgtt tcctacaaag
     7801 ttcaatctag ctgttctaaa ctcaactttg cataaaacca cgtataagct ttattctaac
     7861 ataattctat aaaattgagt agcgaaaatc cgtgggggaa gctgtattat cattcattaa
     7921 tgtaaacgta aaaagtctga ttattaatac tagcaatcag accttgttac aatgattaaa
     7981 ataaatttct agggttaatc ttagcttgct taataaccct cttcataggt taaaacactt
     8041 tcttactgtt ggccccctca aagtacttac gttcatagta cgtttaaaac aaaaaacact
     8101 agaaaatttt ctagcgtttt tacttgactc tgttgtctat taacgacgaa gtccaagtga
     8161 ttgaatcaat tcacggtaac ggttaacatc tgtacgacgt aggtatgcta ataagttacg
     8221 acggtgacca atttttttca tcaatccacg gtaagtagcg tggtcttttt tgtgttgttt
     8281 gatgtggtca ttaaggtggt tgatttccca agtaagaact gctacttgaa cttcaactga
     8341 tcctgtatca ccttcgtgac gtgcatattg agcaatgatt tcattttttt tctcttttga
     8401 gattgccata atatattctc cttttattgc ttcatccgag tcttaggatt ggcactccta
     8461 aaaccaagaa aaagttggtt tgtcttatag accctcttat tttatcaaat tagaataaac
     8521 tttgtcaaaa gtttttaata gaaatatgga taaccaactg tctctaaagg aaaatccttc
     8581 atagactaaa cgagactttg catctttttg ccgaaagacc ttagcataag tagatgtcat
     8641 actagttggc ccacacataa atatcgttgg ttgtccttca aaaacagact gactaaaatc
     8701 aagtcttcct tggaggctac tattatttaa gtgaagttta aaattaggat tggctttagc
     8761 ataggattct aacatatctt gataaatcaa attgtcttga tttgaaaatg tataaaagaa
     8821 gtcaactctc ttagtcaaaa tactattttc tctaataaat gaaatgaagg gagtgatgcc
     8881 gatacctcct gcaatccaaa cctgttcttt cttgtcttta tcaaataaca tatgaccata
     8941 agctctatct aaggctattt ttgtcccaac ttttagctgt ttgtaaatgg atttcgtata
     9001 atctcctgaa gctttaacag ttaaaaatat cactctatca tgaccacccg atatcgagaa
     9061 aggatgagct gctgattcaa agccggcttg ataaattttt aaaaaagtaa attgtccata
     9121 gtcatatcga taaggacgct tcattgctat ttcaatctca gttgtatcat ggttaagatg
     9181 agtaaccttt tgaacgtacc ctacacgacg aaaacgcatt ctagaatata ggaaaataat
     9241 ataaaatcca gatataaccc caatgacagc ataacctaat acgattaaac tgagtaaagt
     9301 attccctagt atcctatctc ctaaaatcag aaaagtatgt accaatccca aaatatagac
     9361 taaataaaca aagcgatgaa taaaacgcca aatttcatac tttaaaaaat ttccgaaata
     9421 agctacaaaa acaatactta gaaaaagata taatcccgca ctgccaatag tctttgcaaa
     9481 ctcacttcca tgtccgcctt gtacaagacc aattttgtgc aatagaagca aaatcattga
     9541 aaatacagcc attaccttgt ggtaaaaata catattttca ataccttgaa agtaactttc
     9601 aagtatccta aatttggttg ataagaggaa tgttagcgat aaacttgtca aagctaatcc
     9661 tggtacaaca aattcactta atcctgcatt ttgccatgct actagtgtca ccactatgct
     9721 agataaaatt aaaacaatac ctttccaagc tcgcataacg accacccctt tatttttagt
     9781 aagaataaaa taccacaaga tacttataca aaacttaaaa atttcagaaa ataacaaaaa
     9841 ggcaactcca atgttgcctt tttgttatga tttataaatt gatttgcaat gctctactat
     9901 atctttaaca gcgagtccat actcctctaa taaatattcc atctgaccta cctgaccaaa
     9961 tcgctcatca atacccatac gactaacagt agtgtctttc tccatactaa gccattcgca
    10021 taatgcactt ccgataccgc caatacggtt atgattctca atagttacaa tggattgatc
    10081 tatcagtaat ggtttcagtt cttctggaag gggcttaatt ttaaaaaggt cgataactga
    10141 ggcctcaatc cctagttctt tgaggtagtc ggctgcttct atcgctctag aaaccataat
    10201 accacttgct acaagagcaa tatcttttcc ctggcgtaat tgtataaaac cttttgaaaa
    10261 atcttcgcat ccctcataaa ctgcagtggg ggccttacga atagttctaa tgtattttag
    10321 accatcaata cttaatgttt gttttaaaat tgcttcaaac tgaatatcat cactaacctc
    10381 aaaaatagtc gcctttggaa ttaaccttag tagacctaac tcttcaaaag gcatatgagt
    10441 tccgccattc atttctgcgc taactcctgc atctgagcca ataatcgtag ctgataattg
    10501 tgagtaacct aacgataaaa atacttgatc aaagacacgc cttgacgcga aaggaccaaa
    10561 agtatgaagg tatggcttgt aacctttaat ggcgagccct gctgcaagac ctaccatctc
    10621 agcctccata atacctagat taatataacg ttttccaaat tctgatgcca gtacatttgt
    10681 tgacattgaa ctcgacaaat cggcttctaa aacggtaatt tgtttatttt cttgattagc
    10741 ttgaaggagg aaatcacgat agacaagcct catttctttg gttgaacatc tcattctacc
    10801 acctccaaac tttctctcaa gcttatcacc gctcgctcta acattgtttt ttgttgtaaa
    10861 tctggtcgta aatgatgatt agaagctaac tcctccaact ctttcacacc ttgtcccttg
    10921 atactatcta aaacaatgca ttttggtttg acactatttg aatcttgaaa agttttaata
    10981 gctttgtcaa ttgcctcaat gtcatctcct tttacacgta ctgcatcaaa tccaaaagct
    11041 tcaaatttag caacaaagtc tccaggatta caaatatcag ctgtcaaacc atctaattgt
    11101 tttttattat catctacaaa aacaattaaa tggtgcagtt gatgatgcgc agcaaattgt
    11161 atagcctccc aacattgtcc ttcatttaat tcaccatcac ctacaatagt ataagtataa
    11221 tagcttgaat tctcaatctt ttgagcataa gcaattcccg ttgcaatact aatgccttga
    11281 cctaatgagc ctgtcgttac atctatacca ggagttaaat tgcggtcagg atgcgaaggt
    11341 agtttggtac cgtttgtatt gagcgaatga aggaatgttt tatcaaaaaa accttttaaa
    11401 tagagtgtac tatacaaggc tggaccagca tgtccttttg ataagaccat ataattccga
    11461 tcactttctt tgaatttttc agggtttata tccataatat caccgtacag aatagccaaa
    11521 gtctcaacaa ttgataaact acccccatag tgaccgaatc ctaaatggtt tagcgtttct
    11581 agtgtattta aacgaatctc cgttgcaaat cgtcttagag atgaatatct ttcttctggt
    11641 gatgtcatta ttaaccttcc ttatgggttg attttccaac aaatgaaatg ccaaaaagtc
    11701 caattaagat aactacaata ccaataacaa tagctatcgc accgccgaca tgattaagag
    11761 cgccaagaat gataccggtc aaaccaaaat cagcatctga gaatgttgaa cctttgaaac
    11821 caagtccacc tagtacaggc atcaagaaaa tcggcaagaa actgatgagg ataccttgaa
    11881 caaatgcacc aatagtcgct cctctcacac caccagaagc attaccgata acacctgccg
    11941 ttgccccaca gaagaaatga ggaacgaccc ctggtaaaat gacagtagtg ccagtcacaa
    12001 tcataactat catactaacc aatccaccaa caaaacttga gataaaacca atcaatactg
    12061 cattaggtgc ataagggtaa acaattggac aatccaaggc tggttttgaa tttggaacca
    12121 atttttctga gattccctta aaggctggta caatttcacc taggataagg cgtacacctg
    12181 ctaaaataac gaatacacct gctgcaaatt gcccagctag ttggagggca taaacaagac
    12241 cattgacacc gttacttatt tcttttgcaa tataagcctc accagcaaac actgcaacga
    12301 taaggtaaat aattgccatt gagatagtaa tacttacggt actgtcacgt aaaaatgata
    12361 aactctttgg aaatttaata tcttctgttg attttgattt atctccaaca attccaccaa
    12421 taaatccact caaccaatag cctaaagaac caaagtgccc aagtgccacc ttatcattac
    12481 ctgtcagttg aatcatgtat ttttgcacaa atgcaggaga tacgctcata ataatcccta
    12541 acgctagact tccaaaaagg atgagcgaga acgaagtaaa gcctgctacg gcaaaaataa
    12601 cagcaatcat acaagccata tacaatgtat gatgtcctgt cagaaaaata tatttaaatt
    12661 ttgtaaaacg agcaattaaa atattaaaaa tcattcccgc taacataatc aaagcagttg
    12721 ctgagccata cttcgtaaga gctactgcta caatggcttc attattagga actaccccca
    12781 ccaaatgaaa agcatgttca aacatttttc caaatggatt caaggaattt tggactatcc
    12841 ctgcacctcc agaaaccact aagaagccaa caaatgtttt tattccacct tttacaatat
    12901 caggaacacc ttttttctgc agtactaaac cgataatggc tatcaaggcg actaaaatag
    12961 ctggcgttct ggcaatatta actaaaaaat ccaatagacc tttcatggca tcaacctcct
    13021 agattaagcc tttttcttgg caaatacctg tcactaattc tctcaattca tccatatcaa
    13081 tgatactgtt taaaatacga acatccccca ggtgaccagc tgaatcctcc aaatcacgac
    13141 caacaatcca aacatctgct gcgcttggat ctgctccacc taaatcataa tgttcaactt
    13201 caacatcaga aacccccaaa tccttcaaaa tagactcaat attcatttgt accataaaac
    13261 ttgaacctag acctgatcca catgctgttc caattcttaa catctttaat cctcctgttt
    13321 tataatcatt tcaatatctt gatacccacg tgaagctaac aaagctttaa cattattatt
    13381 atcacgaagt attgttgtca aatgcgatag agctttcaaa tgcgtttcat tatcaatagc
    13441 tgctatgcat aataagagat aaatttcctg tttggggtca tccaataaat aaataggctg
    13501 ttccaaaaca agcattgaca taccgacaga gtttacccca tcttctggtc tggcatgtgg
    13561 aatagcaata cctttaccaa gattgataaa aggaccaaac tcttcgacct tttcaatcat
    13621 agcttctggg taactttccg taatttttcc agaagctaac aagggttttg ccgctaaacg
    13681 aattgcctct ttccaattca tttgttcaga acttgtctga taagtttcct ctgttataag
    13741 ttggtgcaac aagggcctca catcctttct aaatatatct tgttttacca ttgtcctcaa
    13801 agccaatttt aattcctctt cacaatgaac atgagcgtat ttttttatag ttgctattaa
    13861 ttgatcaagt tggaaatcgt caaggcaagc tttgggaaaa tctgaaataa ccagttcttt
    13921 gagctgttgt acttgctctg ctgtcatcat cagcgaaact agataattag gtttttttgt
    13981 ttctacaaat atagtagaaa agaccatatc gtaagtttga ttgtcaatca gtttcagctg
    14041 ctcaatttta gagacacgat gaaaatgaat ttgaggaaat aatccacgca atttttcctt
    14101 aatcactaag gatgaactaa cgccgtttgg acagagtatc aaagctttat aagacattga
    14161 ttgtgtatca cctgattgtc taagatagcc tccaaagtga atcacgaagt aagaaatttc
    14221 actatcgggt attaatccta cttgctcttc caaaggacgt agtgcctttt taaccaatag
    14281 aaataaatca ctgtaatgct ctttaatatt ttgtgtataa ccactatcgc cagttaaacc
    14341 atatttcaat cggaaataag ctggaataat atgacgcttc agaccttgta ataactcttc
    14401 tttattggaa aaattaagaa gtgaaatagt ttccatctca tcaacgattg cttttgccaa
    14461 tgcttcaaag aaatcatcat ctttagttcc ctcaccttca aaacagccag ataagatcaa
    14521 ggtaatataa tctttttctt tctgatctaa tggtttagat aaggaaatat cctgacttaa
    14581 attagtcaca ataatagtcg ttaactcttt taattgttca gaaacaatag gagaaccctg
    14641 caaaacacga tctactgacc tttgataacg acacagtatt aaaatgataa aatataaaga
    14701 tttctcaagc ctatcttgta ttggagaaag ttgaaaagat tcatagaaat attctaccgt
    14761 cttttctaac ttctcatatg agagagcgaa tttccaagaa gacaagatat aattcaaaga
    14821 ccaaaaacca atgggagatt ctagtagttg agttatcatc tgaaaagcaa cttgatgctt
    14881 atccatctca tctcctacta gactatatcc ctttgctctt gtataagtga gtgagatacc
    14941 tctttttgct aatttagaac gtagcatctt gatatctgat aaagtagtat tcttacttac
    15001 tttcaaaaaa tcttgataat gcacattgga gacaaactct cttcggcaaa agctataaag
    15061 gtaaatcaga catacccttt cttcctgagt taggtaaaat tgctcagctt ctaataagga
    15121 gacaatttta tcatgctctt tttcatctcc taataactga taacgtccag cagagtgagc
    15181 aatagctgtt aggtgatttt ttgctaaaaa actattgaga tagttaattt gctctatgat
    15241 aacttttatt gaaaactttg tttgcactgt taactcatac aaggaaagat ttttccgagc
    15301 taaaaatacc aacattatca caacagtctt attatcaacc atttgttttc ctttctattt
    15361 ttattttata gtaaccgttt acatttttct aggtcatttg agcccaatct ggcactacta
    15421 tcaattgaat gaatttgggc tattgttata gagtaaagaa aaaagaaaga aattgcaaaa
    15481 tctagagcaa gattccttcc cacaagcagc acttaaaaaa cctttactta attgaataag
    15541 taaaggtttt ttaaatgtta tgacactaag atagctaaag tttgataagg ttgcaaagta
    15601 accgtttcat caagagtaac atccttataa tttgatagaa gaacttgtcc ttggagataa
    15661 ttctccggta attttatctt aactttctca gcaaagaaat tatttaaaac aagaagtttt
    15721 tccttatcaa gatggcgttc aaaagcatat actttttcgt tatctttgaa agctgcttta
    15781 taattcccgt cagctattat tggcagttgt ttacgtagcc ttatcaactc ttgatagaaa
    15841 gtaaatatca gccctgtttt ctcctttgcg acattgattt ctttatatga ttttcctact
    15901 ttcaaccatg gagcgccttc agaaaaacct gcattcgtac tatcatccca ttgcattggt
    15961 actcgagaat tgtctcgaga tttcgctcga ataatgctaa atgcctcttc ttgtgacttg
    16021 ccttcatcaa gcattatttg gtaggcattc aaactctcaa tatccacata atcatccata
    16081 gaactgtaat cgggatcaag cattccgatt tcttcaccca tatatatata aggagtacca
    16141 cgcgataagt ggatagatgc tgctaacatg gttgctcctt catttcgaaa acgtttcacg
    16201 tctacaaaac gatttaatgc tcgcggttgg tcatgattat tatagaataa agcattccaa
    16261 ccatttcctt cgctcattcc ttcgccccaa ctatggaaaa ggtcacgtaa tgcaggaaaa
    16321 tcaaaagcca taatggtcca cttttgccca tctttataat ctactttgag gtggtgaaaa
    16381 ttaaaagcca tggataactc ttcacgttct ggtgcagtgt aaagaatgca atttgcaatt
    16441 gtcgttgaag acatttcacc aactgtcatg aaggaatcag cttgtccaaa actagcatta
    16501 ttcaacatct tgagataatc atgtgttatt ggacgatcag tataagctgg ttttccatca
    16561 ttaattgggc agttctcaag tatctcatct ttcccaatta aattaatcac atcaaatcga
    16621 aaacctttaa cccctttatc acgccaaaaa ttgacaactt taaaaagttc cttgcgaaca
    16681 tcggcattgc gccaattaag gtcagcttga gtgatatcaa aaagatgaag ataatacttc
    16741 cctgtatcac caaatggtga ccaagcattt ccaccaaatt tagataccca atctgttgga
    16801 ttatcacgta agataaagaa atcttgatag tagcgatctc ctgcaagtgc ttttttaaac
    16861 cactcatgtt ctattgaaca atgattaagc accatatcaa gcataaaatc tatacggtat
    16921 tgacgtccca cctcaatcat ttcttcaaaa tcgtccatcg ttccaaaatc agggttaatg
    16981 gctgtataat cagagatatc atatccatta tctcgttgtg gacttggata aaatgggttg
    17041 agccaaacca tatcaatacc aagttctgct aaatagggta atttttcgat aataccacga
    17101 agatcaccaa ccccattacc agttgtgtct ttgtatgatt ttggataaat ttgatagaca
    17161 actttacgtt tatcaatcgt catattctgc tcctttccca aaatgctgct gtatttagca
    17221 gtctaactat aacatcaact atcatcaaaa atataacatt tttacagcga tatttgttcc
    17281 catgagtttt ttcttatgtt caaaataatt caaggagcta atgtattaag cccctcaaac
    17341 aattcctcaa attacctctc attttataag agtgctcttt tttaaaaagg atacctaatt
    17401 acttttaaat tttcttggca accattaatg cttgtccacg ttttatctgt ttgggtaaat
    17461 ctatcaattc ttctgatcgg aattcttctt gattagtaat cagaatagga gtttccacaa
    17521 tgtatccttc ttcttttatc attggaatat cgaaagtaat gagtttatct cctactttta
    17581 cattgtcacc ttgggcaaca tgagaggtaa acccttttcc ttctaaatta acagtatcca
    17641 taccaatatg aataagaaac tcaacaccct ctgatgttaa taatccaata gcatgtttcg
    17701 tagggaaaag aactgaaact gtcgcatcga ccggtgaaac tagttctcca tcacttggat
    17761 cgataaccac tcccttcccc ataattcctt gtgaaaagac aggatcacta gcttgatcca
    17821 gtggtttagc taaacctgat aaagggctta ctacactaag ctttgtacct gagacgacag
    17881 ctttttcttt tgctgatttt gtttctgttg ttgaagcaat gacagcatca gatactaatt
    17941 tttcctcttc agtttttgtc aaaatacctg attttttaaa gaatagtgtc aaaaataaag
    18001 gaataaagat agccactgcc atacagataa agaaatatcc catatattta acattaattg
    18061 acaggaagcc tggaagaccg cctacaccaa tagagttagc ttggacatta aaagttgtcg
    18121 ccaaaagacc tgccacactt gaaccaatca taccagcaac aaatggataa atgtatttaa
    18181 cgtttacacc aaaaagcgcc ggctctgtaa caccaaggta ggctgagata gcagctggaa
    18241 gtgatatttg agcctctttt tcatcatgac gatgcataaa ataataagct agtacagctg
    18301 aaccctgagc aatatttgaa agagcaatca tcggccaaag gccagttgta tgtgtttttg
    18361 tatctgctat gagttgagta tcgatagcat ttgtcatgtg gtgcaaacca gtaataacaa
    18421 atggggcata cagggcacca aaaatagcac caaagagcca ttttacagga ccagtcaatc
    18481 caataagaac gatagcagaa atccatttac ctagtgtcca accaataggt ccaaggacag
    18541 tatgagccaa gataatagct ggaactaagg atagaaatgg cacaaaaatc attgaaacaa
    18601 cttcaggaat atgttttctc cagaagattt ctaagtaaga caggctcaaa ccagctaaca
    18661 gagctggaat aacttgagct tgataaccaa ttttttgtac tgtaaagtaa ccaaaattcc
    18721 aagaccaatt tttagcaatg tctgctgctg aagtactagc tacagaataa gcattaagta
    18781 attgtggcga cactagacag atacctagaa caataccgag gatttgagta gtccccatct
    18841 tacgagtcac agaccataca ataccaactg gtaagaaatg aaatatagct tcacctggta
    18901 accataaaaa agagtctact ccgctccaaa acgttgaaac atctactaga gtattccaaa
    18961 ttggatgccc agaagagtct acttgcctga caccatcaac aactttttga cctaaaaatt
    19021 caaatggaac ggcatctaaa atattacgaa aaccaaggat cagaccacca acaataatgg
    19081 ctggaataat cggtgtaaat atttcagcca gcattgtcaa cacacgctgt aaagggtttt
    19141 gatttttttg ggcagccgat tttgccgcct ctttagacac accctcaata ccagaaacgg
    19201 ctacaaaagc attataaaaa atagggacat cattaccaat aatgacttga aattgcccag
    19261 cattggtaaa ggtacccttg actgatggta actcctcaat tacctttacc ttagctttac
    19321 tactgtcatt caaaacaaat ctcatgcgtg tggcacaatg tgtcactgca ctaatatttt
    19381 ctttaccccc aattgcctct aacaaggctt tggcatcatg tttaaattgt tccatctgtt
    19441 tatccttatc tatttaacgg agagtctgtg actccaaggc agaaacttaa agaagactag
    19501 cttacaatat aattgtaaac gattacatat ccctatgcaa atccttagct tatcttttta
    19561 aaaaatctat aaattataaa ataattagtc aataataccc aaaacattag aatttctcac
    19621 caacgttacc aaaatgttag agtcatgagt aggtcagtca cccccaatac tctcttaata
    19681 aaaaacacct actaattgat atagtaggtg agttttttaa tctccattga aagatacgat
    19741 acgattctct tcaatcatat agcattggtt acaaagtttc tctaacatag cgaaatcatg
    19801 actaataacg atgagaccta aatttctatc ctttacaatc tccaacaggc ttttccatac
    19861 actagcttgt gtaatactat ccaacatagt agtcatttca tctgcaataa ggtatttagt
    19921 ctctggatgt aatgaacgca caatcgaaaa gcgttgtaat tcccctcccg agagttcact
    19981 agggcgacga tttagccatt tttcttgaat tccaaaagca tctagtaaat ctcgacttgg
    20041 gtaataagct tcttctaaac tttttttcat aggccataaa ggattcatcg ttttttcagg
    20101 atgttgttga ataagttgta caggtctaaa tgctttgtta ggcaaattac tgccatcaac
    20161 taatacttca ccagatctag gatgtaaaaa acctgcaagc actttggata aactagtttt
    20221 tccacaacca ctttgtccaa atatacctaa aacttgacca ggtgctacct ctaaattaat
    20281 ctccttaaaa agccattgat cttttttatg ataaaagcca agctttttag cctctaaggt
    20341 catgagtaac tcctttcaaa aattcttgct gtgggagaga gcgccataaa cttctagcaa
    20401 attctgtttg taactgctct ccatttccgc taaagaaact agctggagct gtttcaatag
    20461 cttttccctc tttaaaaata gtaatacgat cagcaatttg actagctgct acaatatcat
    20521 gagtgataaa tataacgctt attcctttat ctgcaaagga gcgtagttgg tctaaaacca
    20581 tttgcagagc atctggatgt aatccagggg tgggctcatc cgcaataatc aaagaaacct
    20641 tatcactaat acacgttgta aacaaaacac gtcggagcat tccgccagaa agttggaaag
    20701 gatacaagtc accatcactt tcttttaaac caaactgttg aaacaatcct tcttgagtag
    20761 cctttgaatt ttctgagata cctaagcgca cctgatgttt gactttcata gatggatcta
    20821 aataattaac tgattgtgga atcaacgtaa tatcttttcc tcgcaactgt ttaatgcgtt
    20881 tagaatttag tgattgacca cgataaatca tatctcctgt tacagatgca tttttaggaa
    20941 gaatatccat aatagcatgt gctaataaac tcttccccga accactagca cctataatag
    21001 ctaataactc acctttttta atttctaaat tcagcgcttg tatcggtgtt gattgaaatg
    21061 gttttaaaaa tcttccgtat tgagtgaagg tgatggagag gtctttaatg cttaataatg
    21121 tttctgtcat atctctacct cctaaaaatg atcagtttga gggtaaaaga gtttctttaa
    21181 agattctccg atagtatcaa atgcattgac aaccaaaata agataaagtc ctggaaagat
    21241 aaccaaccac caatttccaa gagagatatg cttagctgcc tctgacagaa tgataccaac
    21301 cgaaggttgt tcggcagaga gcccaaatcc taagaaagtc attgatgctt catgtaggat
    21361 gacatgtgga aataagagga taaaaccaat gaaaatttga gaagcaatca aaggcaggat
    21421 atgatgcctc acaatataat aaggcgtttt tcccatactt ttagaaagtt ggacaaattc
    21481 tttattcttt agatgataga cttcattgcg gataagcctt gctaaagaag gccaatgtgt
    21541 aacagccgtt gcaatgatga ccccttgagc acctttccca acaacaaaag aaatgagaat
    21601 cataaaaatc aaatgaggca taccaataaa caaatcaact aaccatgcta ttattttatc
    21661 aataatgcta tttcctaaac ctgcaagcac tccaaaaact gtcgccagaa tgacccccat
    21721 aagggcacct aataagccga cttgtagaga gaaataaagt cctttaatcg ttctgacaaa
    21781 catatcccta cctaaaccat ctgtcccaaa aagatggttt aatgaaggag caaggttccg
    21841 taaagctgca ttggtttgca agggagtact atggaaataa agatttaagg ctagaatact
    21901 aagaatgaga gaaatggcga tacccagttg ccataaaaca atagttcgac gtctcaatat
    21961 catacttttc tccttaactg tggattgatt atgctattta aaatatccgc aataagattg
    22021 cccgcaaaaa caaataatgt ccctatcatc acaatagcta gaagtagcgg tgtatcactt
    22081 ttaagtcctg cttcagttag ggtagaccct agtcctggat atgagaaaac ttgctcagca
    22141 agaacggatc ctccaaacaa ttctccaaaa taggaaaaat gcagtgtaat agctggtacg
    22201 atagcatttc taagacaatg atttttaaaa atttgccatt ccgtttcccc acgcgctctg
    22261 gcaaataaga catattcact agaaagtacc gacatcattt tagttctagt atgaagagtt
    22321 acattggcaa tgcctagaat acttagcgtg aaaacaggta aaataaggtg cttaatacga
    22381 tcagctaacg taatatcttg actcaaagta cctatcggag aagaaatacc aatcgggaac
    22441 caccccagct ggacagaaaa gattaataaa aaaatgagcc caatccaaaa agttggtact
    22501 gatatctgaa ggtaagaaaa ccaccttaca attcgatcaa gcaatttccc ttgatggaaa
    22561 gctgataaag ttcctaagat aaatccaata agacccgata agacccagga gagtcccata
    22621 agtatgaaag aggcacctgc ccgtgatcta ataatatcaa taacaggttg ccgataaact
    22681 agtgaagtcc ctaaatgtcc ctgtataaaa ttttttaacc aaataaaata ctggaccgga
    22741 gctggcttat ccaagccata gtggtgagca atcgctttgt actgaacagg ggttagtgac
    22801 gtgtcataat tgacacttgc catgactgga tcaaccggtg attgttttag taaaataaag
    22861 gtcaaaattg atactccaaa aacgagtgac aaacacctaa gtatcttcca gataactaca
    22921 gtggtaatat gtttcacaga taaatctcct taattgttaa aaaaggagtc gaggaaacca
    22981 ttcacaccat aaggtgtaca ttgttttctc tgactccaaa cttaaagtag cctacattaa
    23041 ctaaattagt tacttagcag attcatccca agtccattca gcaatgttag tcaataatga
    23101 ccaatcatga ccatgactat ggacaccttg tttacctaca ttgatacgtt tatcaccaat
    23161 ataagtatgg ttaagactca ccaaccatac atttggtaaa tctccaagag tggaagcacc
    23221 tgttttgcca tcccactgag ctaacttcca atatttgtta gctttatcaa ggtcgggaga
    23281 tgtcattgct ttgtcaaggt acttagtcac agtaggattg ttataaaaag taatattggt
    23341 ccaaccttta ccagctaaac ttgggtaatg tgattcataa aattgctgcg catgatgacg
    23401 tcctcccgca taaagtaagg ctgaatcatg tgacttagtt gccatttcat cccagttact
    23461 agccttgagt ttaatagtaa tacctaaggc tttagcttgc tccgctactt caacggctaa
    23521 gtttgctcgt aattgatcat tagtagggta gtaaaggtca aattccgctt taagatttcc
    23581 ttttttgcgg ctaccgtctg cttgttcttt ccatccagct tttgtcaaaa gttgcttagc
    23641 tttagctact ttattatctt taattgctgt ttttggattc cagaatggtg ttctatcaat
    23701 aattgaataa gctggtttac cataaccatt taaaacagta tccagaactt tttgtctatt
    23761 taaaccaata gtcaaggctt ttctgattgc tggatcacta gtgacatcat ttcctactgg
    23821 ataaccgtct ggtgaatttt tcacaacacc tttcttcaca taaggtagcg ataacccacg
    23881 aacatcattt gaagcaatat ctagaagacg tgttcctttg actttcttgc tagcaagctc
    23941 tggcgttgcg taaatcatgt caacatcacc agattctaaa gcagctagtg ctgtattttc
    24001 atcaagtaat acccaagtcc actttttaaa ataaggcttt ttaccgtgcc aatatggatt
    24061 tcgaacgaag attgcttgtt ctccagcctt atattctttt accatgtaag gtcctgaacc
    24121 gataggattg ctcttatact tatcattgta atgtttttta gggacgattg ggatttctgt
    24181 caactgtgct gtaaatgtcg aatgcgcctc agtcaaatga atatttacct ggtttttccc
    24241 aactacttca acattcttaa taaaggtcaa atcccaagct tttccatctg ctttcaacat
    24301 atcataagta aacttaacat cgtcagcagt aacaggctca ccattggaga atttaaaatc
    24361 atcatttaag tcgaacgacc acgttaagcc atccttagag attttatatt ttttagcaag
    24421 ctctcctttt atatctagtt caggagaacg tttcaatagg gtactatgag taatattacc
    24481 ttcattatgg attccataac ggtcctttgg atcgaattca tgaggaagct ttgcccccat
    24541 agagacaacc aattcatctt tgggtcgttg ttttcgtgtt cgctcctttg tttgtgaatt
    24601 ttgattttga caagctacta aaaagatact cgccagaaac aaagcaacaa aagagatgta
    24661 cttcaaatat ttagtcacta ttaaacctcc ttttttaatt gataaataat atttttgaca
    24721 acttttttca ttataacaag aaaaaagtag gagaacaatt acttaactgg ttaattagaa
    24781 aatttgttat tttatcatta ataacttaaa gttaggtaaa acacaattct agactctagc
    24841 tcataaacca tgttaatcca atgtatttta atagacatca taaaaaggct gaataaattc
    24901 agccttaacc acgctattag ttatagcaaa ccaatcaaat gactaaagat aggaataacc
    24961 aaatcaacta ccaaaccgat aaccaccacc gctatagctg ccatcgcacc ttgcagttct
    25021 cccaattcca atgcaaaggc tgctccgact gtatgaccag aggtccctag acctaatcct
    25081 gcaccaatct tactatcatt caaatggaaa aatgacacta attttttacc taatgcataa
    25141 ataataactg catttaagat acacgccatt gctgtcacag ctgtgttccc tccaattgct
    25201 gctgttattg gaagagcaat tgctgttgtt gctgcttgtg gcaacattga agcaattccg
    25261 acttgactaa gcccaaccat tttagaaata gcgacgataa gcataaggga aacaatcata
    25321 ccgattacta aactgctgag aatttctacc caatattttt taacaacgtc atttttctta
    25381 tagagaggaa ctgcaaaagc aattgttgct ggatttaaaa accaaaatat taaatcgcca
    25441 cctggtttat aagcttgtgt ataaaatgtc ttaacatcgg ttcctaaacc ttttgacata
    25501 agccaaagaa tgacaatacc actaaccatt gcaaagaaaa gaggctgcaa aaggaagaaa
    25561 cctttactct tcttaaataa atgttgtcct atcgtataga gtattaaaga aaagcaaata
    25621 ccaaagatgg gtgtttttaa gagttccata ttatttttcc tctactttct tactatagtg
    25681 gttaggtaat atctttttaa ataaatctgt gacactgagt tctctgatag caagtattaa
    25741 tcgtgcaacg tatgctacca ctactaacat aacaacagtt gagattgtaa taacagcaac
    25801 tagttgcaag ccttcagctt ttaggatatc aaggttcgct gctactgata ttcctgaggg
    25861 gacaaacata aaaccaatca tgcttatcat caaagcccca aaagagtcaa cccactctac
    25921 cttgataatt ttagcagtta gcaatacgta cattagcacc aaaccaataa ccgtcgttgg
    25981 gattggtagt gatttcggta ataacattga aatcatttgt gaaactaata gaatagctgc
    26041 atatattgac atttgaataa acattggagc ttcttttgtt ttggtttctt tactggtgtg
    26101 tttcatagtc actacccctt ccttttatta caatgtttta acttgtttta ttgatagcta
    26161 tattgtactt caaaataaat aaaacgtttt cattttcagc ctcagttacc aaaaaaagaa
    26221 gccgaattgc aaattcaagc ttctaaacaa caaagggaag ttcgcaaaag cacttttaca
    26281 tggttaaatg ctcatttagg tcttttaaat acgatcgacc tactggaaat ttctctccat
    26341 tagacataat tagtaacaaa gtatgattaa accaaggttg aatttcctca atcatctcta
    26401 gattaatcac tgtgtttcga tgaatctgga gaaaatatgg tgctacggcg cgtgacttaa
    26461 accaattgag tgttttccga atagtgtaag ttttctgaac tgtactaaca gttaattcgc
    26521 catcttcaac actagctgca acgatatctt gcattttaac aacaacactg cgatctgata
    26581 atgtcaaggt taacaattca gcactggctt ttttaggtat tgcttgctct acatctgaag
    26641 cagtggtgag ttggctcaat ttcttcactt ttgatagagc catatcaacg cgctgttgtt
    26701 caaacggttt catgatataa tcaacagcat tactttcaaa agctttcaca gcgtagttat
    26761 cataagctgt cgcaaaaaca acaagtgggg gatgtgcaag ttggctcaac tgattagcca
    26821 aagtaaagcc attttcttca ctaagcgaaa tatctaaaaa aattaaatca atttgctgtc
    26881 taaataagat tttttcggct tcactgatat cctcagcctg aaaaatctca gggttgtcaa
    26941 cctcctggct atgttcaact aaaaacgata attcttgtct ggcaaacatt tcatcatcaa
    27001 gaattaaaat tttcatgctc atcctccctt attctattag gtattcgata ccaaactttt
    27061 gtaccattct tgtcgctcga aaaatgaagg caacttacac taccatataa taaattcagc
    27121 ctgttattta gattaactag agcagtacct gtccccttac tctctgcaac tgtttcttga
    27181 cctaatttat caatgatagt atctgagatt ccttgtccat tgtcactaac agaaacacaa
    27241 taataatgac catctggctt tatttgaacc aatatatggt tgtccgtctt acgttctttg
    27301 aaagcatgtc gaactgcatt ctctaccagt acctgtaaac caaaaggcgg taacttcatt
    27361 ttttctggtg cactaatatc ataagataac tgatatttat cagggaaacg taatttttca
    27421 acattcatat aagcatccac atgtgatttt tcttgctcaa gcgttacctc acgatcctga
    27481 ccaccttgca aacttgttct aaaaaaagta cttaactgca tcagtgcata acgtgcttta
    27541 tcagaatcaa tacggattaa tgcactaatt gtgttaatgg cattaaagaa gaaatgaggg
    27601 ttgatttgtg cttgtaaagc ctttatctct gccatactgg ctaacttatt ttgttcctct
    27661 gttatcccca ttgccagttg tcctgaaaat atttgcgcta aaccaaggac taggttttcc
    27721 tccacctcag acattgtctt atctcctgca aagtacattt ttaaggcaca cacagtttta
    27781 tcatttattt ttagaggaac tacaatagca gaatttaact gacagttgtg atctggacaa
    27841 gaaatcgccg ctttatcttg cgcaattctt ggttcgccat caaaaataac acttttagat
    27901 aagtctgttt tgaccggttg tcctgcaata tggtgatcat ggccaacacc aatatgagct
    27961 aatacgtttg accgatctgt taatcccaca gcatcaaagt tagtatgcct ctttataatt
    28021 tcgcaaacgc tcctagcaga ttgcggtgtc aaaccttgtc taaggtaggg cagagtctgt
    28081 cgagtcaatt caagaacatc tctcgtttga actgcgcgta actgactttc atttgacaaa
    28141 taagttttca aaatcgcaag gaaaagtgtg gaacctaaac tatttaaaat catcattgga
    28201 atgacaatca ttttgacaag ttcccatcct gtaaaaatgc caacaaatag catctggata
    28261 ctttcggcaa taatactaat taaaataact tggcttgttg aagggtagag atggttttcc
    28321 ttaagcttat caccaatctt tccgctaaca atgccgacta gaactgaact gacaatatag
    28381 aaagaacctg aaaagcttcc ttgaaaaaag cgatgaactc ctccaataaa accaacaatt
    28441 gatccaacca gaggtccacc aaccaaactt gccgttgtaa taactaaagt ccttgtatta
    28501 gcaagtgagt cagaatggga aatcgttgtt agaaaagggc gctcgaccaa acttcgatcc
    28561 ccttttattt caattcctgt tatattagat ataataacaa acaagccgaa aatgatgaca
    28621 aggactaccg tttcacgttt agaccgctct tcaattaact gtctgaaata actattattt
    28681 accaataaaa aggctaaaat cataataatt cctagccttt ggaataacaa caccatcaac
    28741 atatgcttat cctcctcaag ataccatttt actccggaag ataaatatag atatcttgat
    28801 aatctgaaat aaccttgtct gccattgata aatcttgcgg tggataatca ggattggcaa
    28861 agccaaagca atacattcct gctgccttag cagctaaact cccatttctt gtatcttcta
    28921 taactataca aactttaggg tctacatcta aaagctctgc agctctaaga aaaacatctg
    28981 gtgctggttt agaagacgat acatcttctc ctgtaaccat gtattcaaag cattctgtca
    29041 cacccaactc ttttagatta cgcttaatat ctaccattgg agatgaggac gcaactgcta
    29101 agcgatagcc atgttggtgt aaccaatgaa tcagttgctg agctccttta atgggcctaa
    29161 caccatcacg cgtcacaatc gcttgtcgac gtctgttcat ctcagcaata tactctttta
    29221 ctgttttcgg taagccaaac tcctctttca tagcttgcca catgaattca aaagtagtcc
    29281 ccatgtactg gtattgatac gagacgtccg tatcaatacc ctcctcacgt aacatttctg
    29341 tcttattatc taaaaacgtg tactcggaat caacaataac gccgtccata tcaaaaataa
    29401 tgactttttc catcttaaac ctgctttcta tcccctttat tgtaatcgtt tgcctttcta
    29461 ttttcaataa aaaaaggttg ctttctgtgg aaagcaacca gtatatttag aaatgatatt
    29521 ttgagagtga ttttatttaa aaggggagct cctcttcttc caatactaaa tctgacaaat
    29581 caccagaaac attattttca cgcatagcac gttgggcacg gctttctaac aactgaaatg
    29641 atgatgctaa tacttcagtg atatagtgcg tttgaccatc cttttcgtac ttgcgcgtac
    29701 gcaattcacc atctattgaa attaaagagc cctttgtccc atagctcgca agggtttccg
    29761 ctagacgacc ccacataacc acattaataa aatctgcttc acgctcacca ttacttcctt
    29821 taaagcgtct attaacagca acagttgcac gcgttactga cttgtcagtt ggtgttttta
    29881 ccatctcagg ctttgctgtt agacgcccaa tcataataac tttattatac attgattacc
    29941 tcctcactat tattattcga aaataaggaa gaaaaatgct aaaatatatg aaattgttct
    30001 gggagaatta agatgacaaa tgtaactagt tataaagaaa tgctggctaa accttgggga
    30061 aaaatacaat atgaaataac ttttgctcaa ttaagccata tcaaaaatca gaacgtttta
    30121 gattttggag ctggtttttg tctcaccgca caacatctag caaaagaaaa caatgtaact
    30181 gctattgaac ccaatcctaa actgctttac gataatcaaa gtgacaatat ttataaaatc
    30241 ctaggttcct acgaagcatt aagagctttg cctgatcaaa gttttgatac tattatttgt
    30301 cataatgttc ttgaatatat agacaaacat aatcatcctg cttattttga cgaattttct
    30361 cgacttctaa aacctaatgg agaactatct ctcatcaaac ataatataat tggtaaaata
    30421 ttacaatcag ttatttttag caatgatacc tccactgcga tggaactctt aacaggagaa
    30481 gcaaacttta aaagtgccag ttttgaccag ggaaatattt ataccttaga agaactaaaa
    30541 caaaatacaa atttattagt tgaacgttat cagggtattc gtaccttcta ttcattacaa
    30601 ccaaaccatt ttaaaactga aactgattgg ctaaacaaaa tgcttgctat cgagctaagt
    30661 gttgctgaca aagctcctta taaagatatt gcttttttgc aacacatcac acttaaaaag
    30721 tcattgtaac ttcttaagtt tacaactaaa aatcaactta gctgaagcta agttgatttt
    30781 tactaatttt tccaatgttt attggcgtcg aaagactctc caacaattac ctgatcagat
    30841 aattcaatga tacctctgac ttgaggagca tttggaagag ctaactcgcg agcactacat
    30901 aacattccaa agctatcttc tccacgaagt tttcctggga aaataaggct accatttggc
    30961 atcatagctc ctggaagagc tgcaactgtt ttaaggccta ctgaagcatt aggtgcccca
    31021 caaacgattt gaaccgtctt cccatcgttg atttctactt gacagatgtg taaatggtca
    31081 ctatctggat gatcaatcat ttctttaatt tgcgccacaa caaatgtagg ttcaatatta
    31141 ttaacaagag agtcttcgaa tccttctttc agtagttctg cattaaggat tttaataact
    31201 tgatcagata aagttacttg acctactccc tcaatagcaa ttaaacttga cacttcaaag
    31261 atattccaag caactgtttc tttactatct tgcagataga cgcgtgctac ttgtccgcga
    31321 cgatcaacat ctagcttagc tccttgacta tccttaacaa taaccattaa ggtatcacca
    31381 acatgctctc tattataagt aaaaatcatt tattctcctt taaattttgc tattttttgt
    31441 cttatttatg gttaatttca gcgagaaatt tagtaatttc agcctttgtt ttgcgattct
    31501 tattaaccaa acgacccagt tcctgaccat tttccacaac aacgaaactc ggaatcccaa
    31561 aaatattcca ctgctgagct aattctatat agtcatcacg attaacacgt acaaagacaa
    31621 aatctgagaa atctttttca attgatggca ttactggata tataaattga caatctggac
    31681 accaatctgc tgtaaaaaag aaaacaactt tcttagtgct atcaatataa gctgctattt
    31741 cttcataaga ttctggtaaa atcatttgct cacctccaca taatcaagaa caccttgacg
    31801 ataaatgaat tcgaaaattc tgccatcttc caagacaaga ccaccactgg tcattttaat
    31861 atcagattca aattcattaa aataaaatac ctgaatatca cccatctgac taaaaagttc
    31921 tcttaaggtc tgagtgattt gctgacgttt ataatctgtt acttttttag ttagggtata
    31981 accagcagct gccagtaaac ccacaccagc aaggccagtc ccaaataaga tttttttatt
    32041 tttcataact gttattatac catacccccc ggaattagac acaggaattg atggaagaaa
    32101 tccacataac agttatgaaa aaaaacaact tattccttgt ggtataatag gatgacattg
    32161 tcttaagata ccattatggt taatcattca aacagaaaga aaaaacatga aagaaaaatt
    32221 attagtacct gctttaaatt gtttgctagg tctactcttc ctaggtgatc actggttttt
    32281 atttcgtctt caaaatggag aactttttgc tattgtgacg ctaatactgc tttgcacagc
    32341 ttcattttta atcaataaga atcaccccaa aaatttgact ttaattgcta tggttatcac
    32401 ttttttagcc gtattatctt tcctatctac ctttttatta gcttaagccg ataaacatta
    32461 tctccgttgt attcaaccca gttttcacta taacaggctt tgtcagtcat aacagtcttc
    32521 tggtagtact tgatattctt tttgaaagtc tttatattga ctgaaacctg ttctgttctt
    32581 tatttttaat taaaaggtgc taaaatagta tttgtagaat tgaggtaatt atgtcagatt
    32641 tatttaacaa aattaaaacc gtaactgagc ttgatgggat tgctggctat gaacacaata
    32701 tccgcaactt ccttcgtcaa gaaataactc ctttagttga tcaagttgag acagacggac
    32761 ttggtggaat ttttggagta aaaaatactc atgagactaa tgctcctaaa gtcatggttg
    32821 ctgcccatat ggatgaagtc ggctttatgg ttagtcatat tcagccagat ggaacatttc
    32881 gtgtacttga ggttggagga tggaatcccc tagtagtcag ctcacaacgc tttaccctct
    32941 acacacgttc tggtgatgcc attcctgtta tatcaggctc agttcctcct cactttcttc
    33001 gtggacaaag cggtggaaca acattaccca aaattagtga cattgttttt gatggaggat
    33061 tcacagataa aaatgaagct gaaagctttg gcattgctcc tggcgatatc attgttccta
    33121 aatctgaaac cattttaact gcaaatcaaa aacatattat gtcaaaagct tgggataatc
    33181 gctatggtgt gcttatggtg accgaattgc taaaaagctt aaaagatcaa agtcttagca
    33241 acacacttat tgctggggct aatgttcaag aagaagtcgg acttcgtggc gcacatgttt
    33301 caacaactaa attcaaccca gatatcttct tagctgtcga ttgttcccca gctggagata
    33361 tttatgggga acaaggcaaa ataggagagg gaaccttaat ccgtttttat gatcccggac
    33421 atatcatgct taaagatatg agagatttct tgcttacaac agctgaagaa gcaggtataa
    33481 aataccaata ttatgctgca aaaggtggta ccgatgctgg ggctgctcac ctaaaaaata
    33541 gtggtattcc ttctacaact atcggtgtct gtgcacgcta cattcattct catcaaacac
    33601 tctacgctat ggatgatttt ctacaagcac aagcttacct tcaggccatc gttaacaaat
    33661 tagaccgctc gacggtggat attattaaag gttattaaga tagaaaggat gggagtttca
    33721 taaggtagac acattttagt atctcctttc atccccatat caacattatg aaaattggaa
    33781 ttatcggcgt tggaaaaatg gccagtgcaa ttattcaagg cctcaaacaa acccaacatg
    33841 acattgtcat atccggatct tgcttagagc gttcaaaaga aattgcagaa agacttgatg
    33901 ttacttacgc cgaatctcat cagtcattaa tcaatcaagc tgatattatc atgttaggta
    33961 ttaaacctca actatttgaa aaggttcttc ttccacttga tatcacaaaa ccaatcatct
    34021 caatggcagc aggaatctcc ttagcgcgtt taagccaatt aactcgttct gatttaccat
    34081 tgattcgtat tatgccaaat attaacgcac aaatacttca aagttgcaca gctatttgtt
    34141 acaacaatca tgtatcagat gagttacgac aattagccaa agaaattacg gatagttttg
    34201 gaagtagctt tgatatagca gaaatcaatt ttgatacctt cactgctcta gcaggttcta
    34261 gtcctgccta tatctatcta tttatagaag ccttagctaa agcaggtgta aaatatggat
    34321 ttccaaaaga acaagcactc tctattgtag gacagactgt cttagcttct agtcaaaacc
    34381 tattacaagg acaaaatagc acctctgact taatcgacaa catttgtagc ccaggcggaa
    34441 ctactattgc tggcctttta gacttagaaa aaaatggtct gacgcatagt gttatttctg
    34501 ccattgatgc tactattgag aaagcaaaaa aactttgagc aaattgctca aagttttttt
    34561 agttattcat cattgtactg attaacacgc tataagcaag agcattgtta ataaaatgaa
    34621 tcattatact atattctaag tgctcagttt tataataaac aaaagataaa atagcaccca
    34681 tcccagcata aataataaaa ctacctatat cagaagggga atgaataaga gcaaaagcta
    34741 aagtaccgat gacaaaaccc catttttgat gtttcgcaaa aagttcttta gggataatat
    34801 agcgaaatat aatttcttcc ataattggtg cccctattat ggcaaaaatt aacatcaaag
    34861 ctagtggaag ttgctttgtt aattccataa ttactgtttg atttgctgta tctttaaccc
    34921 cttgcatagt cataatgagg taaccaataa tttgtggaac cctcaatgat aaccaaccta
    34981 agagaatagt aactaatgct ttccttgtca ataatacttt ccatctgatt atttgtttaa
    35041 ccttggcata ataaaggaaa agagcaataa taagtaattc taagccaatt aacaatatac
    35101 tagtaaaagg cgtttgatag tgattcttta ggagccacac agccaatata ggaacaagtt
    35161 gattaataac taaaaatgct attagcaaag ctaaaatttt gcctttttta ataataaatg
    35221 acacaaaaac ctccaaaata aattattaac ttttttctat cataacatat tttcaaatct
    35281 tatagattta tctcacaata acttctgtaa aataaagtaa attaataaat aaccgtataa
    35341 aacaaaagag agtatcaata gcaagaactt tgcataggaa aaagttatac caaaccagac
    35401 attaatcaga cacaatacca ctaaaacaag gacagataaa aggaaaagga gtcgacaaga
    35461 catattagca ataaattgat tacgctcatc aaaaactttg gtatagtaat gttgaaatcg
    35521 cttttcacta aatgttatca ccaaaaaata aaaacttcct gcaaataagg caatacctga
    35581 accaaaggaa aaacctttag taaaattaga ttcaaaggta tgtagaaatg atgatactat
    35641 cataatcaaa ctacatacac taaaaacaaa agacaaaaat ctcatatata ctttgtaatc
    35701 cttcggagat actttactat tgggtgacaa taatcctttt attaacatag aatactactt
    35761 tctaaaactt caatttaatc atcagctatt atatcgtttt cttcaatgaa gcatagcgat
    35821 aaatagcaaa aatagacatc gcaatgctaa tactaatagt actgataaaa acatattgct
    35881 ggtaaacgat tggtggacaa aaatcaatag tccacatcct tgacatatta cagattaact
    35941 tttaaccttt tgtcttttgt ggactgtatt tttccattaa aattctatcc tccttcactt
    36001 tctgtataaa taaaaacttc ctcaatttgt ttatcaaaat aacgcgctat cttaaatgct
    36061 agctccaaag atgccgtgta tttctctttt tctagagaaa taatagtttg cctagtaact
    36121 ccaagtgcaa ctgcaagttc tgcttgactt aatttcctag attttcgtaa cttttgtaga
    36181 gaatttttca tatcttacac ctttttgtat agttagcttt acatttatag tatagttaac
    36241 tatacatttt ttgtcaagta cattttacat acctgttcaa aaaacaaaaa agttgatagt
    36301 taaactatca acttttctgg tcttatcaat tttttgataa cttatttagc tttaaggcgc
    36361 tcaacgtcac gcgcaatcat taattcttca tcagtaggaa taactaaaac cttcactttt
    36421 gaatctggtt ttgtgatatc tccaaaataa ccaaaaacat ttttttctgg atcaagttca
    36481 atgccaaacc atgataatcc tgcgataaca tcttgacgca ttaatggcgc attttctccc
    36541 atacctgcag taaaaataat agcatccgcg ccatttaata ctgccaagta ttgtccaata
    36601 aatttcttaa tacggtcaat aaagacatta taggctaaca ctgcatttgg atctttactt
    36661 tgaagacctg cttcaatatc tcgcatatca cttgaagtgc ctgatactcc aagcaatcct
    36721 gattgtttgt taagcatatt aacaacagca gctgcatctt ccaattctgg atcattagct
    36781 acaagataag ggataatcgc aggatcaatg tctcctgaac gtgttcccat cattggtcct
    36841 gcaagtggtg tgaatcccat cgatgtatca atagactgtc catgataatt tgctgtaata
    36901 gaaacaccat tacccacatg tgctgtgatt aattttaatt cttctaaagg acgtcctaat
    36961 tgcttagctg cttcttgtgc aacgtattgg tggcttgtcc cgtgtgcacc gtatttacgt
    37021 actttataat cagtatagta cttttgtggg attggatata agtaggtatg aggttgcatt
    37081 gttgtatgga aggctgtatc aaatacacat acactcgtga tatctggtaa gatttctcta
    37141 aacgcacgaa tccctgctgc agctgctggg ttgtggagtg gagctaaagc tgataattct
    37201 tcaacctgtt ctacaacttt atcatctact aaagcagact ctttaaaata ttcaccacca
    37261 gcaacgacac gatgaccaac acctgtaatt tcattaaagt ctttgataat accatgtttt
    37321 gttagatctt ctaaaagaat tttaacagct tgggtatgat ctacgatatc taaaatttgt
    37381 tcatcttttt tatcgtcaaa ttttacagtc gaaatagaat ctttcaaacc aatacgttcg
    37441 ataatccctt tggcaactac tttctcctct ggcatttcat ataattgcca tttcaaactt
    37501 gaacttccag cattaattgc tattgttttt gacataattt acctctctaa agcgttttca
    37561 caatctctat tctatcatat tttttgagta atgactattt tctctgctcc atttttgaaa
    37621 attttcaatg aagtttgcca tattctcacg attttgcaaa tctgtcagcg gatatacaaa
    37681 ggtttctggt ttttgttctg cttgcttctt gagaacaaat atagatttcg cattttgacg
    37741 acttccaaaa atagtttctg gtagagttaa aacggcaatg acatctgcat atccttttaa
    37801 ccactccttc agcaaatcac tttgtggact tgttaaaagg ttttcgggtg ctaaaaatat
    37861 agcgattcca tcttttttca aatatttaag agattgctcc atcaatagat ggtgagcata
    37921 ggtgtgctct ttagaacttg atacagcata tcgtttagca atatcatcat taggataata
    37981 gccaactggt aaatcactaa tgattacatc gctttctttc aaaatttgtg gtctaacggc
    38041 atcctcttgg ataaattggg cactagaacc tataatttca gcaatgcttg ctgatagatc
    38101 aatcaaaaga tcatcaactt caatgcccat ataatttaac tctttcgagc tgttattgag
    38161 gagagtctga gctaaattcc cagttccact tccaatttcc aagacatcca ctgtctcttg
    38221 actcgttaat tcttccaaaa gaaataacaa gataaaacca atactatctg gcgtaaactg
    38281 atgattagct tgtaattgct ctgtttgcgc agatttgata aaaatgaact ggaaagtgcg
    38341 acgccattct tcttgactta agtcaagttg acgtaatttt tggttattca ccacaacaat
    38401 atctaaatca caacttgaac caaggtaata agagttctgt tcaattaagg catcataaat
    38461 atgagttttt aattggttct caatcgtttg gatattttct aaaatcagct cataggctgt
    38521 ctcaattttt tcaaaattca tgatttcctc cagaatttat cataacaaaa aagacagcat
    38581 ctgaaaagag aagcaaacaa aaatcctgtt tttaagcaaa accttgtctt actaatattt
    38641 catcactgta tatttttaat gtttatggct tgctttatta tcattagact tgagaggtac
    38701 ttggagagtg tctattttac gataaaactt atggttatag tttcctccct tatcctatga
    38761 tactgtcttt ctgttgccaa tattcttgct agataaaact gtaagacgag tgtaaataca
    38821 gcagccaata caatagcttg caaaaggatg ccagctttaa gtttcttctt taaaatcata
    38881 gtaaaatgtc ctttttaacc cgtccttaaa ataaaaaaca agttttacca tacttttggt
    38941 ctgactcatt tgacaattgt ctaacccata aaccattggt tgataacctc gaccattata
    39001 acctgtctta cggaaatcat ctttattaga tttgccaaag gttacaatct tatcttgctt
    39061 acgtaaataa agtttgttct gtcttaaata ttccaagtga gcgccttcga attctgcatt
    39121 tagttgctga gttaataaca cccattcaga ctgactggaa gaagacatca ctactatctg
    39181 ttgagccaac aattttgtta gtccttgata aactagcaaa gctcctgtga ttgtaaccaa
    39241 tgctaccaaa cattctaaaa tgtgtaaatg ctttaacctt cttatcctta cattttaaca
    39301 ataaattttt catgataata tctccatatt ttgatacatt ggtaacagca ttgctgcata
    39361 aatcataaca atgataagag ctacaaaaat aaaaataacg ggttggatta attgggtcgc
    39421 tctagctaat tttgtaaaaa aatcctccca cttctcatca gcatatatat ctaactctgt
    39481 tcctaatttc gccttaactt gaccatattc aatcattaaa ctaagctcag ttaagaaaaa
    39541 cggatagtct aatacttttt ggtgaaatgc tttacctgat agaaaacctt cttccatgtc
    39601 atatcctatt tccctaaaaa gtttggattt ctgattttgc attactttaa caatttggtc
    39661 caattcaaca ccttgactta ataaatttcc ccactcacgg gcatagtaag cagttaaata
    39721 aagcttaaca tatgatccaa ctaagggaat tgttgttaaa aaacaagcta ctttaatgcg
    39781 cgacaatcgc ttttgaataa tataaaatat taaactaaaa ataagtacaa ctgctaaaag
    39841 taataagaaa atattcggca catttgtaat cagtctagtt gcaaaattat tttctcctaa
    39901 ttggggcatt aaataatgcc taaggccaat cataattagc accagaaaag acaataatat
    39961 caatgggtaa gtagctactt ctattacttt ttttctaact aacaaaagat tagctaaata
    40021 agactcaatc tttagtaggc tccttgaaat gttaccatgc aaatcagcta atgcaacctg
    40081 tgtgataaca gtgtcagaaa aacctaactc tcctaacatt tttgataaat cttttccctc
    40141 taataatgcc ttattcatcc tatctgtata acaatcagac aataacttac tcctctttaa
    40201 gaaggtgacc atatcagtta aagaaaaacc actagcaaaa agattattaa aaagttggac
    40261 tactttccgt tgtttcttga gggataattt tttcgacttg tgcctgtttc ttactgatat
    40321 gtccttcttc agccaagata tccacttgtc tattccactt gtctgatgag tgttttttaa
    40381 agttacttgt ctcaaagtca attaggcttc ctcctccaat taaacgttga tatgctatta
    40441 attttagact attttctaac tcttgatagt taacccctaa ttctataagc ctatcataga
    40501 ctccgggaat acttttagca tgaatagtag aaaaaaccat tactcccgtt aaacttgcac
    40561 gaataacagc acgggccgtc gcttgatctc taatctctcc gataattaaa atatctggac
    40621 gatgccgtaa agacagtttg attaaagcat cataagtcat tccaatatcc tcattcaatt
    40681 ggagttgtaa catcttgtca ttcttgattt ctaccggatc ttcaatcgtg ataatttgct
    40741 tatttttaaa tacttctgaa gctaattgat acatgagagt tgttttacca ctccccacag
    40801 ggccggaaaa aagatatagc cctcttgcac acagtacttc cttcatttgc tttatattat
    40861 caaaccaata ttttaagtcc tgatgacctg aatacaaagt acgaataact aaagattctt
    40921 gaccacgata atctcccaca cttgatagtc gtaatgaaac cagtcttccc tctgacagtt
    40981 catagtcaca agaacctaat tgacttcgtc ttttttctcc aacgttcatg cctgccacaa
    41041 atttaaagtg actaataaga ctagccatcc tattaaactc aaaaacatca ataaaccgcc
    41101 tttcatcatc aatacgcata tagaattcat aacaatcacc tttgggaatg atataaatat
    41161 cttgagcatt tacttctact gcctgatgaa tgacgtgctt tgctaatgat tgaaccatcc
    41221 cgtacctcct taaccatata ttcgaaaata aaataaaaaa atctcaccaa ttggtgagat
    41281 tttttcaatg atttcttgag gggtagtaac ataaatatta tgaagcagag gctgaacaaa
    41341 cctattatat ccccataaaa actatctaac ttttttaaac tacttttaat tacaaattta
    41401 aagaacatgc cgatggcaac tcagctatct tattaaatac ttcaaatcgc tcaatagatt
    41461 cttccttagg aatttcttgc cattctttca ataacattaa ggaatgatac tgttggaggt
    41521 actcatcaca ttcttcacac caacgttttt cttttatact ccaaaacgtt gccaacaaac
    41581 tagacttact ttgaaaataa ggccactttt cctgtcccct atcccactcc tcttgaaaat
    41641 agagcaaagc ttctgataag gtatcatact cagcaatttc agtaatatct tcctcccaac
    41701 cttcaataaa ccaccatggt tcccaatcac caaacatttt tacaacttga tacataatac
    41761 acttccttta gagagtatta tataaaaaat aagaataaaa taaaaaatat ctgctcattc
    41821 caaattcttg taaaaaaaga acctgagtta aactcaagtt ctttttgatt aatcttctgt
    41881 tgtaggtgtt gatacttccg taacttctgc atcaacagga gttccttcaa taatctcaac
    41941 ttcattaaca gcaagtggtt caatattacg gtaacgagcc ataccagtac ccgctggaat
    42001 gattttaccg ataataacat tttctttaag accaagtaag tgatctttct taccacgaat
    42061 agcagcatct gtaagaacac gtgttgtttc ttggaaggat gcagctgata agaatgaatt
    42121 agtttcaagg gaagccttag taatacccat aaggacagga cggcttgtcg cagggattcc
    42181 accagaaata acaatatctt tgttagcatc tgtaaagtct gaaatatcca taagtgtacc
    42241 tggaagaaga tctgtatctc ctggatccat gacacgaact ttacggagca tttgacgaac
    42301 cattacctca acgtgtttat ctccgatttc taccccttgg ctacggtaaa ctttttgtac
    42361 ttcagcaagt aggtacgttt caacagataa agtatcacga acttcaagaa gacgttttgg
    42421 ttggatagat ccttcagtta aggccgcacc acgagctact tcatcaccaa cttcaacttt
    42481 catacgtgct gtaaatggaa caacgtattc tccctcacct gtctgacctt taacgaatac
    42541 tttcttagta cgtgttgaag agtcttcttc aattgcaacg acttcaccct taacttcagt
    42601 aatgactgcc tcacctttag ggttacgtgc ttcaaagatt tcttggatac gtggaagacc
    42661 ttgtgtaata tcagtatttg aagcaacacc accggtatgg aaggtacgca ttgtaagctg
    42721 tgtccctggt tcaccgatag attgagctgc aatagtacca actgcttcac caacctcaac
    42781 agcatcacct gttgccaagt taataccgta acagtgacga caaacaccat ggcgagtgtt
    42841 acatgtaaat actgatcgga tagttacttc ctcaacgccc gctttaacaa ctttagcagc
    42901 catatcttca gtaattaagg tatccgcacc aacgagaatt tcacctgtct caggatgttt
    42961 gattgacttc ttagtataac gtcctatcaa acgctcttca agtgtctcag taacctcttt
    43021 accatctgta attgctgtga ttgtcaaacc gcggtctgtt ccacagtcat cttcacgaat
    43081 aataacatct tgggcaacgt caaccaaacg acgagtaagg taacctgagt cggctgtctt
    43141 aagggccgta tcagtcatac ctttacgcgc accgtgagtt gagaagaaca tttccaaaac
    43201 actaagacct tcacggaagt ttgataagat tggcaattcc atgatacgtc cgttaggagc
    43261 agccatcaaa ccacgcatac cggcaagttg tgagaagttt gagatgttac cacgagctcc
    43321 tgagtccatc atcataacga tagggttctt tggatcttgt gtctcaataa gacgtttttc
    43381 aagtgcttct ttagcttcac gccatgttgt tgtaacagca acataacgat cttcttcagt
    43441 catcaaacct cgacggaagg ctttattgat atcttcaact ctgtggtgag ctgcatcaat
    43501 aatctcagct ttattgtcaa taactgggat atcagcaata cccacagtta aaccagcaag
    43561 tgttgaatga taatatccaa ggtccttcaa acggtcaagg aaagcagaag tttctgttgt
    43621 acggaaacgt ttgaaagttt ctgcgataat attaccaaga tttttcttct taaagggaat
    43681 attaatctct aagttatcaa taactgcttg aatatcctga cctggctcta agaagtattt
    43741 atctggagtt ttttctgtca agtttgcatt attaggttca attaagtacg gtaaatcttc
    43801 aggcatgata tcgttgaaga gaatcttacc aactgttgta accatgattt tatgtttttg
    43861 ctcttctgtc caaggcttat tcggcattga atcaacagcg ataccaacac gtgtatgtaa
    43921 atgaacataa ccattttgat atgccataac tgcttcgtca tgatctttga aaatcatgcc
    43981 ttcaccttca cgaccagcat cttccatagt caggtagtaa tttcctaaaa ccatgtcctg
    44041 agatggtgtt acgactggct taccatcttt agggttaagg atgtgctctg cagcaagcat
    44101 caaaagacga gcttctgctt gggcttcttc agacaatgga acgtggatgg ccatttggtc
    44161 tccatcaaag tcagcattgt aggcctcaca tacaagtgga tgaagacgaa gcgccttacc
    44221 atcaataagg acaggttcaa acgcttggat acctaatctg tgaagagtag gtgcacggtt
    44281 tagaagtact gggtgctctt taataacttc ttctaagatg tcccaaatac gctcgtcccc
    44341 acgttcaacc atacgtttag ctgctttaac gttccctgcg aggtcacgag caacgatttc
    44401 acgcattaca aaaggtttga agagctcaat agccatttca cgtggtacac cacattggta
    44461 cattttaagt gttggaccaa cagcaataac ggaacgtcca gagaagtcaa cacgtttacc
    44521 aagcaagttt tggcggaaac gaccttgttt acctttaagc atatggctta atgatttcaa
    44581 aggacgacta cctggtccgg taattggacg accacgacga ccattgtcga taagcgcatc
    44641 aacagcttct tgtaacatac gtttttcatt ttgaacgatg atgccaggag catttaattc
    44701 caacaaacgt gccaaacgat tgttacggtt aataacacgg cggtaaaggt cattcaagtc
    44761 tgatgccgca aaacgaccac catctaattg aaccatcgga cgaagatccg gtgggataac
    44821 cggtaaaatg ttaagaacca tccattctgg tttatttcca gattttttaa aggcatctaa
    44881 cacatccaga cgacgaactg ctttcacacg tttttgacct gtcgctgatt tcaactcctc
    44941 tttaagaact gcgatttctg catctaaatc aacgcgttta agaagatctt gaatggcttc
    45001 agcacccatt ttagcaacaa acgaaccgta accgtactct tgaagttttt cacggtattc
    45061 acgctcagta aggagtgact tcggttctaa tggtgtgtcc attggatcga taacaacata
    45121 agccgcaaaa taaataactt cttcaagtgc acgaggactc atatccaatg tgagtcccat
    45181 acgcgatggg atacctttga aataccagat atgagatact ggtgctttta actcgatatg
    45241 tcccatacgt tcgcgacgaa cttttgcacg agttacttca acgccacaac ggtcacaaat
    45301 gattcctttg taacgaatac gtttgtattt tccacacgca cattcccaat cttttgttgg
    45361 accaaaaata acttcatcaa aaagtccttc acgttctggt ttcaatgtgc ggtagttgat
    45421 tgtttcaggt tttttaactt caccataaga ccatgaacgg accttacttg gtgatgctaa
    45481 tgtgatttgc atacttttaa aacgatttac gtcaaccact agttttacct ttcttcgatt
    45541 tatcatcata ctagcccagc taggatttca ataaatccta ggactagcat tgcatatcaa
    45601 ttctatattc gcttctagtt ataaactaga taaatttaat cttcttgaac gacttcagat
    45661 acttgttcag cttgtttttc ttcagcttct tgtttcacac gcgctttttc aaggtcatca
    45721 acgtgcatca catcatcatc ttcaccttca tcaagatcac ggagttcaac ttcgttgtca
    45781 tcttcatcaa ggacacgcat atcaagacca agtgattgca attcttttac aagaacacgg
    45841 aatgattctg gaacacctgg ttttggaatt ggcttacctt ttgtgatagc ttcataagct
    45901 ttaagacgtc ctgtaacatc atcagatttg tatgttaaaa tttcttgaag aacatttgaa
    45961 gctccataag cttcaagcgc ccaaacctcc atctcaccaa aacgttgtcc accaaattga
    46021 gctttacctc caagtggttg ttgagtaaca agtgagtatg gtcctactga acgggcatga
    46081 agtttatcat caaccatgtg gtgaagtttg atcatgtaca tgacaccaac cgacacacgg
    46141 ttatcgaatg gttcaccagt acgaccatca taaagaacag tcttagcatc tgaatccata
    46201 cctgcttctt gaacagtttc ccaaaggtct tctgaagaag ccccatcaaa gactggtgtt
    46261 gcaatatgaa tacctaaatt acgagcagcc atacctaaat gaagctccat aacttgtccg
    46321 atattcatac gtgatggcac cccaagtggg ttcaacatga tatcaactgg cgttccatct
    46381 ggtaaataag gcatatcttc aacaggaaca atacgtgaga cgacaccttt gtttccgtga
    46441 cgaccggcca tcttatcacc gactttgatt ttacgttttt gagcgatata aacgcggaca
    46501 agcatattaa cacctgattg caattcatca ccatttgcac gagtaaagat tttaacgtca
    46561 cgaactactc catcgccacc gtgaggtaca cgtagtgaag tatcacgaac ttcacgtgat
    46621 ttatcaccaa aaatggcatg caagagacgt tcttcagcag ataagtcctt ttcaccctta
    46681 ggcgttacct taccaacaag aatgtcacct tcttttacct cagcaccaat gcggataatt
    46741 cccatttcat cgagatcacg tagtgaatct tcaccaacat ttggaatttc acgagtaatt
    46801 tcttcaggcc caagctttgt atcacgcgtt tcagattcga attcttccaa gtgaacagat
    46861 gtatagacgt cttctttcac aaggcgctca ctcatgataa cggcatcctc gaagttataa
    46921 ccttcccaag tcatataagc aacaactggg ttttgtccaa gagccatttc accattttcc
    46981 atagatggtc cgtcggcgat aaaatcacct ttttcaacga gatcaccaac tttaactaag
    47041 gtacgttggt tataagcagt acctgagttt gaacggcgga atttttgaac atgataaaca
    47101 tcaagagaac catcttcgcg acgcacttca actttttcag catctgaaaa aataacacga
    47161 ccgtcatgtt tagcaatcac agctgcacct gaatcgtggg ctgcttgata ctccatacca
    47221 gtaccaacat atggtgcttt tggatcaatc aatggcacag cctgacgttg catgttggca
    47281 cccatcaagg cacggttaga gtcatcattt tccaagaaag gaatacatgc tgttgcgaca
    47341 gcaactactt gcttaggtga cacgtcaaca aagtcaacaa tgcttgaagg aaactcttgg
    47401 ttattacctt gatgacgacc cataacgatt tcttctgcaa atgtaccgtc ttcgttaagc
    47461 ttagagttgg cttgtgcaac tgtaaattca tcttcttcat cagcagtcaa ccaaacaatt
    47521 tcgttcgtta ccgcaccagt agaacgatct actttacgat atggtgtttg aataaatcca
    47581 tatttattga ggtgtccaaa tgaagataag ttattaatca aaccaatgtt tggaccttca
    47641 ggtgtttcaa ttggacacat gcgcccatag tgggtatagt gcacgtcacg aacttcatat
    47701 ccagcacggt cacgtgtcaa accaccaggt cctaaggcag agagacggcg tttgtgcgac
    47761 aattctgata gagggttgtg ttggtccatg aattgtgaca gctgtgatga accaaagaat
    47821 tctttaacag cggcagtcac tggacgaatg ttaatgattt gttgaggtgt caagacttca
    47881 ttgtcttgta cagacatacg ttcgcggaca ttgcgttcca tacgagcaag tccgatacgg
    47941 aattggttcg caagtaattc accaactgca cgaatacgac ggttacctaa atggtcaatg
    48001 tcatctacct taccgatacc ctctgcaagg tttaagaagt atgacatctc tgctaaaata
    48061 tctgcaggag ttaaggcacg aactttatct tcaggatttg agtttccaac aatagtaaca
    48121 acacggtctg gatcagttgg agcaacaact ttgaattttt gaagaataac tggttcagta
    48181 actacagcat aatcatttgg tgtgtagaca aacttgttta agtctccatc gatatgctct
    48241 gcaattgaat caatgacatc tcgtgtcata acggttccag cttcaacaag aatctctcct
    48301 gtttcaccat caactaaatt ttcagcgatt gtttggttca ataggcgtgt tttaagattc
    48361 agttttttat taatcttata acgaccaaca gccgctaagt cataacgacg tggatcaaag
    48421 aaacgtgcaa ctaaaagact acgtgagctg tctgcagttt ttggttcacc tggacgaaga
    48481 cgttcataga tttctttaag tgcttcatct gtacgagaat cacttggatt tttatgaatg
    48541 tctttttcaa tagtattgcg aacaagttca ctgtcaccaa agatgtcaac aatttcatcg
    48601 tctccagaga aaccaagtgc acggactagt gttgtaaatg gaattttacg tgtgcggtcg
    48661 atacgtgtat aagcaatatc ttttgcgtct gtctcaagtt ccaaccaagc tccacggtta
    48721 gggataacag ttgacccata accaactttt ccatttttat ctaccttatc attaaagtaa
    48781 acaccaggag aacggactaa ttgtgaaaca atgatacgtt caccaccatt gatgatgaaa
    48841 gtacccattt cagtcataat tggaaaatca ccaaagaaaa cttcttgagt tttaatttca
    48901 ccagtttctt tattaactaa acggaaagta acaaaaatag gtgctgagta gctagcatca
    48961 tggatacgag cttcttccaa agtatattta ggttctttta attcataacc aacaaattca
    49021 agatccatag tatctgtaaa gtttgaaatt ggaagtacat cctcaaatac ttctttcaag
    49081 ccagcatcaa gaaaatcttg gaatgaatca gtttgaattt caattaagtt tggtaaatca
    49141 agaacttcct taattcttga aaagctacga cgtgtacggt gttttccgta ctgaacttca
    49201 tgtcctgcca agtttttagc tcctttttaa agatagagtg actaagtttt tatctcataa
    49261 aacttagtga gtttatttca tttgctcgcc taacaagaac aagcttatag atgtcgtttg
    49321 tggtaatttt cagaaagttt aaatatatat tacaataacc ttttttctta aaattaggca
    49381 caaaaagagc agctaaatct cactttatca agttgattta actgctgtat atctaggtcg
    49441 gacaatattt caacctagac agacgacaaa tagttacgta cattataact tattttcacc
    49501 aataaatcaa gttttattct cttgacttca aggaagcttc tagaatttca atttgttcat
    49561 caacaccctt gagttttagc ataacacgat gatttttaaa catagcatac tggtgttttt
    49621 ctaattcttt gaaagaagac tatacaagaa ttaaatcata ttatcttgga aaaaataaaa
    49681 ggagaacaga tcactctatt ctcctcaaat aaaactatca gtatcgtaaa cctgttatcg
    49741 ttttccaccc aaagtagacc aagctttttg ataatcacta tcagttcccc caatagcaaa
    49801 acgataggtc atcgttccag gtccattttt tgcccaataa cttgttgtgc tttcacctcc
    49861 aacagtgatt cgacgaccgt ttactgttac tacacctggc tgtaaacctg tagacttaag
    49921 aactttagct tttataacgc tatcatcaag tctaaacctt tctgacttac caaaagtatt
    49981 gccatctgca ttattaatag cattaaccaa gtgagccata taattagcat tattattgta
    50041 acctgtcaat ttcgctaaag aggcattatt atcatgtcct gcccatccac ctaaagtcac
    50101 tttaggtgta gaaagcatca accaaacatc agaagttgag ttagtagtac ccgtttttcc
    50161 aatccaatct accccagcta aaccactatt taaaccttgt aagcgatttt taaaggttgt
    50221 agtctttccg gagttaattg gtccatgtaa tagctgctgt aagatagtag ctgttgcttt
    50281 cgaaaagaca cggacaggtt tactttcgtg attataaatc actttcccat tactatcctc
    50341 tatactttct atcatatact gcttatgata aacaccacca tttgcaatca tttgataaag
    50401 gtttgtttgc tgagcaactg acgtatcaat accaccacca agtggtaaac tttctatacc
    50461 aaagttttca attggatagt ctaatttctc catatagttt tttacatcaa cgcctcgatc
    50521 tcgtagcatc ttgtaagtcc agaaagctgg aatgttccaa gaaatgtcca atgactcttg
    50581 aaggttaacc atagcagtac cttcttcatc cgcatgcata atcttttcgc cacttgaata
    50641 tgtcgttgga taatttgaaa gaacactacc acttcctaac atcccttgat caatagcaat
    50701 tccgtaaggt aatatcggtt taatacttga ccctggagag cgtgccgtat caaaagcatg
    50761 gttattttga ttctcactat agttacgacc accaataaat cctataatag caccactaga
    50821 attgtccgtt agtacatttc ccatttgaac cttgccagta ccatcatcta acaaccctcc
    50881 gtattgagct gcagcatcct gcatggcttg ataaacagat ttattgatag tagttttaat
    50941 agtataccct ccctgttgaa tctcttcaat agcgcggtgt ctgtacgtcg ctctagtctc
    51001 atcatttttt aaatcatgct ctgaaacatt atccttttta atgaggtaat tgtacatcac
    51061 tttttgagct tcagataatg ccgagtagta aagataatca tgatgatttg ttgtcgccac
    51121 tgcaggtttg ataaaatctt ttttgatgtc ataatctttg taggatttgt actcatcttt
    51181 ggttaaagct ctcgttctat acatattgta taacacattt ttttggcgct ttataccaaa
    51241 actaagatct ttatctgatt tcaactgggc atctgcagta taaggagaat atacaatagg
    51301 actttgcggt aaacctgcaa ggaaagctgc ctgaggaatt gttaaatctt ttgcggaaac
    51361 tccaaaaatc ccttgtgctg cttcttcaat acctgcaata ttctgtcctt tgttattgcg
    51421 accaaacggt gatacattta aataatctga taaaatcgaa tccttatcca tgtaacgctc
    51481 taatgctaga gcataaataa tttcttttga tttgcgttta aatgacggat catctcccaa
    51541 aatttgctgc tttaataatt gttgtgtcag cgttgaacca ccgctactct ctccaaaccc
    51601 cagaacagat cccgctgcag cacgtagtac ggctttggga actacacctt tatggtcatt
    51661 gaaattttcg tcctctgttg caatgatagc ctttttaata ttatcagata tagcatcctt
    51721 tgccactggt gtacgttgta aatcagtggc aatctctgaa atttgtgatt tatcagaata
    51781 agttaaccta gacaccctag taagtgtatt aacttgtgtc actaaactat ttttacttgg
    51841 tactttaaca gagtcaactt gactagcaag ataccctact gcaagtccta ctccgagcat
    51901 aacaaatagt aaaataataa catagatgaa atctgataaa agctttacaa ttcttaaaaa
    51961 aatagaacca aattcaagtg gtgtgtaatc acctaattta gaactattca acttcttatt
    52021 acctttaaac atgaataata cctcatccta cattatatca gatttaatat aacttgcata
    52081 tcctaaagag ttttgatata gttgacatta atacacaaca agttaataat taggagaaaa
    52141 agatgaatat ttttgatgaa ctcaaagaac gtggactcgt ttttcagaca actgacgaag
    52201 atgctttacg caaagctctt gaagaaggtt ctgtctctta ctatactggc tacgatccta
    52261 cggctgacag ccttcatctt ggacatttag ttgctatttt aacctctcgt cgcttacaac
    52321 ttgctggtca caagccttat gcacttgtgg gaggcgcaac tggacttatt ggggaccctt
    52381 catttaaaga tgttgagcgc agtttacaaa caaagaaaac tgtagttagt tggggtaata
    52441 aaatccgcgg tcaattatct aattttctag agtttgaaac aggtgataac aaagctgtat
    52501 tggtaaataa ttatgattgg ttcagcaata ttagtttcat tgatttctta cgagatgttg
    52561 gtaaatattt tactgtcaat tatatgatga gtaaagaatc tgttaaaaaa cgtatcgaaa
    52621 cagggatttc ctatactgaa tttgcttatc aaattatgca aggatatgac ttttacgaat
    52681 taaataagaa ctataatgtc actttgcaaa ttggtggttc tgaccaatgg ggcaacatga
    52741 ctgctggaac cgaacttatt agacgaaaat caaatggtgt atctcatgtt atgactgttc
    52801 cattgataac agattctaca ggaaaaaaat tcggaaaatc tgaaggaaat gctgtttggc
    52861 ttgatgctga caaaacctct ccttatgaaa tgtaccaatt ttggctcaat gttatggatg
    52921 ctgatgccgt tcgcttcctt aagattttta cattcctaag ccttaaagaa atagaagata
    52981 tacgtattca attcgaggaa gcacctcacc aacgcttagc acaaaaaaca ctagcacgtg
    53041 aagttgtaac tcttgttcat ggtgaaaaag cttataagga ggccgttaat atcacggaac
    53101 agctatttgc tggcaatata aaaggattgt ctgttaaaga gctcaaacaa ggtttacgtg
    53161 gagtaccaaa ttatcacgtt cagacggaag ataacctcaa tatcatagac cttctagtca
    53221 cttcaggagt tgtcaattct aaacgtcaag ctcgagaaga tgtctccaat ggagctattt
    53281 atatcaatgg agatcgtatt caagatttag aatatactat ctcagaaaat gataagcttg
    53341 aaaatgaaat tacagtcatt cgtcgtggaa agaaaaaata ctttgtatta aactttaaat
    53401 agacttgaag tgtaatcgat actctgataa taatatttca agacaataaa aagtgtagtc
    53461 aattgactac actttttatt aatga
//
LOCUS       DATRUK010000007        48360 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.007, whole genome shotgun sequence.
ACCESSION   DATRUK010000007 DATRUK010000000
VERSION     DATRUK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48360
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.007"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..134)
                     /locus_tag="VBE34_000339"
     CDS             complement(<1..134)
                     /locus_tag="VBE34_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEO7263089.1"
                     /translation="MINDINNFIESKKLSLNSRKSYHYDLKQFYKIIGGHVNSEKLAL
                     "
     gene            complement(124..597)
                     /locus_tag="VBE34_000340"
     CDS             complement(124..597)
                     /locus_tag="VBE34_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO7263090.1"
                     /translation="MIAKEFESFLLNHLDHYLIPAEDVAIFVDTHNADHVMLLLASNG
                     FSRVPVITKEKKYVGTISISDIMGYQSKGQLTDWEMAQTDIVEMVNTKIEPINEAATL
                     TAIMHKIVDYPFLPVISDQNDFRGIITRKSILKAINSLLHDFTDEYTITPKNNDK"
     gene            complement(594..1115)
                     /locus_tag="VBE34_000341"
     CDS             complement(594..1115)
                     /locus_tag="VBE34_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO7263091.1"
                     /translation="MAIRKLVVMSDSHGDRDIVKDIKNHYLGKVDAIFHNGDSELPSS
                     DPIWEGIHVVTGNCDYDSGYPEVLVTKIDNAVIVQTHGHLHQINFTWDKLDLLAQQED
                     ADICLYGHLHRADAWKNGKTIFINPGSVLQPRGPINEKLYAVVTITDSKVLVEYYTRQ
                     HQPYPNLTKEFSR"
     gene            complement(1097..2071)
                     /locus_tag="VBE34_000342"
     CDS             complement(1097..2071)
                     /locus_tag="VBE34_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEO7263092.1"
                     /translation="MTKTIFESKTEGNWFLGSFQAFNYFTCFGNDESYEAIQDVFHRL
                     LSTLKVEGLQLHVVQMTSDFQLLAFLVDMINQEYSRHIKVTQHKGAILVSEDDQLFLV
                     HLPKEGTSLEKFFDLKNDNNFGDTILIATHNEGKTKEFRELFGKLGLKVENLNDYPDL
                     PEVEETGMTFEENARLKAETISKLTGKMVISDDSGLKVDALGGLPGVWSARFSGPDAT
                     DARNNAKLLHELAMVFDKERRSAQFHTTLVVSAPNKESLVVEAEWPGYIGTEPKGENG
                     FGYDPLFIVGEGSRTAAELSAQEKNNLSHRGQAVRKLMEVFPKWQLEN"
     gene            complement(2068..2862)
                     /gene="racE"
                     /locus_tag="VBE34_000343"
     CDS             complement(2068..2862)
                     /gene="racE"
                     /locus_tag="VBE34_000343"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEO7263093.1"
                     /translation="MDSRPIGFLDSGVGGLTVVKEMFRQLPEEEVIFIGDQARAPYGP
                     RPAQQIREFTWQMVNFLLTKNVKMIVIACNTATAVAWQEIKEKLDIPVLGVILPGASA
                     AIKSTNLGKVGIIGTPMTVKSDAYRQKIQALSPNTAVVSLACPKFVPIVESNQMSSSL
                     AKKVVYETLSPLVGKLDTLILGCTHYPLLRPIIQNVMGAEVKLIDSGAETVRDISVLL
                     NYFEINHNWQNKHGGHHFYTTASPKGFKEIAEQWLSQEINVERIVL"
     gene            complement(3037..3276)
                     /locus_tag="VBE34_000344"
     CDS             complement(3037..3276)
                     /locus_tag="VBE34_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEO7263094.1"
                     /translation="MSTTIWILLIIVALFGGLVGGIFIARKQIEKEIGEHPRLTPDAI
                     REMMSQMGQKPSEAKVQQTYRNIVKHAKTAIKTKK"
     gene            complement(3434..3976)
                     /locus_tag="VBE34_000345"
     CDS             complement(3434..3976)
                     /locus_tag="VBE34_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263095.1"
                     /translation="MSKKTTQMVSYTSILVAFAIMIPIIMPAKIIIGPASFTLASHVP
                     LFLSIFISVPVAILVALGTGLGFLLAGFPIVIVLRALSHIGFALIAAFLIKSKPSLLM
                     SKWQTLLFAVAINIIHGLLEFITVYIITMTSNSSSTYLWSLFSLIGLGSLLHGLVDFY
                     IALFIWKWMTQKLGKTLRIR"
     gene            complement(4038..4559)
                     /locus_tag="VBE34_000346"
     CDS             complement(4038..4559)
                     /locus_tag="VBE34_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEO7263096.1"
                     /translation="MKAQERRENILTTLKGTKEAISASTLAKIFSVSRQVIVGDIALL
                     RAQQCDIISTPKGYLMSSALSTHQFTARLVCQHGIEQTEEELEIILRYQGIIMNVEVE
                     HPIYGMLTAPLNIQSQKDIDNFTAKLKVSNAELLSSLTDGLHTHMISCQDQSVFDQIC
                     EALKKAGILYIDK"
     gene            complement(4627..5316)
                     /locus_tag="VBE34_000347"
     CDS             complement(4627..5316)
                     /locus_tag="VBE34_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEO7263097.1"
                     /translation="MNDNVIYTQSDSGLNQFFAKIYGLVGIGVGLSAAVSAIMLYMFP
                     QNMIAIMQKMPGLYFGAIILELVLVFVASGAARRNTPAALPLFLIYSALNGFTLSFII
                     ARYTQTTVLQAFITSAAVFFAMALIGAKTKKDLSGMRKALMAALIGILIASLVNLFIG
                     SGGMSYIISIVCVIIFSGLIAYDNQMIKYVYNSQGGQVADGWAVSMALSLYLDFINLF
                     LNILRLFARND"
     gene            complement(5339..5842)
                     /locus_tag="VBE34_000348"
     CDS             complement(5339..5842)
                     /locus_tag="VBE34_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO7263098.1"
                     /translation="MKKYQDDKDFMDLVGHLIDHPRFQKLEAIVQHHHSTRLEHSINV
                     SYTSYKIAKKFGWDASSTARGGLLHDFFYYDWRVTKFNKSHAWVHPRIAVRNARKLTD
                     LNAREEDIILKHMWGATIAPPRYKESYIVTMVDKYWAVREASRPLKRIFKKPIRFSRK
                     FLGSHNH"
     gene            complement(5880..6623)
                     /locus_tag="VBE34_000349"
     CDS             complement(5880..6623)
                     /locus_tag="VBE34_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEO7263099.1"
                     /translation="MTTIITSKSNNLIKKTKKLLQKKYRKSSYLIEGWHLFEEAEKYG
                     AQFLNIFVTETAIDRLRKPERAIVVTDDVLKELTDSQTPQGIVAEIAFQETRWTDIKK
                     GRFLVLEDVQDPGNLGTMVRTADAANFDAVFLSQKSADLYNQKTLRSMQGSHFHLPVF
                     RVEIEQFVNFCKAEGITMIATTLSEQSVNYKNLPKYDYFALIMGNEGQGISKTMTEEA
                     DVLAHIEMPGQAESLNVAVAAGVVIFSLI"
     gene            6655..6933
                     /locus_tag="VBE34_000350"
     CDS             6655..6933
                     /locus_tag="VBE34_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEO7263100.1"
                     /translation="MKKVHLIVSGRVQGVGFRYATYSLALEIGDIYGRVWNNDDGTVE
                     ILAQSTDSNKMTQFIQKIRKGPSKWSKVTYVDIKLDNFDDFNDFKMSN"
     gene            7018..7950
                     /gene="yidC"
                     /locus_tag="VBE34_000351"
     CDS             7018..7950
                     /gene="yidC"
                     /locus_tag="VBE34_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO7263101.1"
                     /translation="MKKTLKRILFSSLSLSMLLLLTGCVSVDKAGKPYGVIWNTLGVP
                     MANLITYFAQHQGLGFGVAIIIVTVIVRVVILPLGLYQSWKASYQAEKMAYFKPLFEP
                     INERLRNAKTQEEKLAAQTELMTAQRENGLSMFGGIGCLPLLIQMPFFSAIFFAARYT
                     PGVSSATFLGLNLGQKSLILTVIIAILYFVQSWLSMQGVPDEQRQQMKTMMYLMPIMM
                     VFMSISLPASVALYWFIGGIFSIIQQLVTTYVLKPKLRRKVEEEYTKNPPKAYKANNA
                     RKDVTNSTKATESNQAIITSKKTNRNAGKQKRRG"
     gene            complement(8150..8815)
                     /locus_tag="VBE34_000352"
     CDS             complement(8150..8815)
                     /locus_tag="VBE34_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7263102.1"
                     /translation="MINWDAIFNLELAVKAFPSVIQGLPYTIGLSLVGFILGAIVGFF
                     VALMKMSHFRLLRYLANIHISLMRGIPLMVLLFLIYFGLPFIGIQLDAVTASIVGFTM
                     MSSAYISEIIRAALLAVDHGQWEAARALGLKTPTIYRGIIIPQATRIALPSLSNVLLD
                     MVKGSSLTAMITVPDIFNNAKIVGGTYSDYMTAYILVALIYWVICTLYAIIQDWWEKR
                     LLY"
     gene            complement(8839..9696)
                     /locus_tag="VBE34_000353"
     CDS             complement(8839..9696)
                     /locus_tag="VBE34_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7263103.1"
                     /translation="MKKTILLGLVGLSAMTLAACSNGQSNKETTWDNIKKDGVLKVAT
                     PATLYPTSYYDDHKKLTGYEIDMMKAIAKKLKIKVKFVEVGVAESFTSVDSGKVDVAV
                     NNFDTTPERLKKYNFSQPYKYSVGGMIVRADGSSKITAKDLSDWKGKKAGGGAGTQYM
                     KIAKQQGAEPVIYDNVTNDVYLRDVSTGRTDFIPNDYYTQVIAVKYVTKQYPDIKVKM
                     GDVKYNPTEQGIVMSKKDKSLKTKIDAAIKDMKKDGSLKKISEKYYAGQDLTKEPAGT
                     DKIPVIKVK"
     gene            complement(9857..11317)
                     /locus_tag="VBE34_000354"
     CDS             complement(9857..11317)
                     /locus_tag="VBE34_000354"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEO7263104.1"
                     /translation="MVFKDATAMVQAIKQHKISSQELVEQAIYKIEEQNVSVNAVVSK
                     QYNEARQAAKYANESNAPFAGVPILLKDLGQNQKGQLSTSGSQLFKHYHAKQTDYLVQ
                     SFEKLGFIILGRTNTPEFGFKNISDGQLHGNVNLPFDHSRNAGGSSGGAAAAVSSGMV
                     PIAGASDGGGSIRIPASFNGLIGLKPSRGRIPVGPSSYRGWQGASSHFALTKSVRDTK
                     RLLYYLQSYQVESPFPLKKLSKESLFEFSVSKPLKIAVLMDSPLKTKVSSEAKAAIKE
                     AADFLSQKGNHLELVEQPLDGIHSMKTYCMMNSVETAAMFDDIEKSLGRSMEFSDMEL
                     MTWAMYQSGQRVLAKDYSKLLDSWDQFAATMARFHENYDLILTAATNQPAPFHGQFDL
                     DETLQKQLRHMGEFSVSEQQDLIWKMFEDSMAWTPFTHQPNLTGQPSLAIPTHLTKEG
                     LPLGVQLTAAKGREDLLLAVAELFEKEKQFKGPVYH"
     gene            complement(11421..11903)
                     /gene="greA"
                     /locus_tag="VBE34_000355"
     CDS             complement(11421..11903)
                     /gene="greA"
                     /locus_tag="VBE34_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEO7263105.1"
                     /translation="MAEKTYPMTQVEKDQLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQIQILETKIRYAEIIDSDAVAKDEVAIGKTVLVQEVGTND
                     KDTYHIVGAAGADIFSGKISNESPIAHALIGKKTGDLATIESPAGSYQVEIISVEKTN
                     "
     gene            complement(11976..13778)
                     /gene="mltG"
                     /locus_tag="VBE34_000356"
     CDS             complement(11976..13778)
                     /gene="mltG"
                     /locus_tag="VBE34_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO7263106.1"
                     /translation="MTEFNDDQHSNHDQKSFKEQILAELEEANRLRKLREEELYQKEQ
                     EAKEAARRTAQLMADYEAQRLKDEQEARAKALETKQRLEEQEKARIEAKLLAEAAREE
                     ERRQAEQALASQEEQVINQGMEPSRELDSGSKSSEFRTTENVPDIDLKADKTDVATAV
                     PNQETEEIFLVRATDIPTEGENVKLGETSELEPVAKEPIRVEDLSKEEEGIALSAKNK
                     HNKRERRQKADNVAKRIARILISIIILVLLLTAFVGYRFVDSAIKPVDSNSNKFVQVE
                     IPIGSGNKLIGQILEKAGVIKSATVFNYYSKFKNYSNFQSGYYNLKKSMTLDQIAAEL
                     EKGGTAEPTKPALGKILITEGYTIKQIAKAIESNKIDTKTTSTPYKADDFLKLVQDET
                     FIKKMVAKYPNLLGSLPDKSKAIYQLEGYLFPATYNYYKDTTLEGLVEDMISTMNTKI
                     APYYNTIKAKNMSVNDVLTLSSLVEKEGSTDEDRRKIASVFYNRLSAGQALQSNIAIL
                     YAMGKLGDKTSLAEDAQINTSIKSPYNIYTNTGLMPGPVDSPSISAIEATIKPASTDY
                     LYFVADVKTGNVYYAKDFETHKANVEKYINSQIN"
     gene            complement(13865..14368)
                     /locus_tag="VBE34_000357"
     CDS             complement(13865..14368)
                     /locus_tag="VBE34_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7263107.1"
                     /translation="MIIRNGCLEDLQQVISIEQINFSEAEAASKKAMQERLTIMTDTF
                     LVAEINGRLAGYIEGPVIKGRYLTDDLFHKVSEFPVRVGGFIGITSLSIHPDFKGQGI
                     GTALLAAMKDLVVSQERDGISLTCHDDLISFYEMNGFKDEGESDSQHGGSLWYNMIWN
                     NPTRSIC"
     gene            complement(14454..15785)
                     /locus_tag="VBE34_000358"
     CDS             complement(14454..15785)
                     /locus_tag="VBE34_000358"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEO7263108.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHNVQGSDVDKYYFTQRGL
                     EQAGVTILPFSPNNISEDLEIIAGNAFRPDNNEELAYVIEKGYHFKRYHEFLGDFMRQ
                     FTSLGVAGAHGKTSTTGLLAHVLKNITDTSFLIGDGTGRGSANANYFVFEADEYERHF
                     MPYHPEYSIITNIDFDHPDYFTGLEDVFNAFNDYAKQVQKGLFIYGEDPKLHEITSEA
                     PIYYYGFEDSNDFIAKDITRTVNGSDFKVFYNQEEIGQFHVPAYGKHNILNATAVIAN
                     LYIMGIDMALVAEHLKTFSGVKRRFTEKIIDDTVIIDDFAHHPTEIIATLDAARQKYP
                     SKEIVAIFQPHTFTRTIALLDEFAHALSQADSVYLAQIYGSAREVDNGEVKVEDLAAK
                     IVKHSDLVTVENVSPLLNHDNAVYVFMGAGDIQLYERSFEELLANLTKNTQ"
     gene            complement(15795..16412)
                     /locus_tag="VBE34_000359"
     CDS             complement(15795..16412)
                     /locus_tag="VBE34_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263109.1"
                     /translation="MNRGSRRFPLIADDEPVMSPLVKMNLYDNEDLINNIRGFYQEKT
                     YQSMVKSNYEHEEISHPKVIENDPVPPQSFVKKATELSKSRQEAKRSVREKRQAYYAK
                     QEFKAPSKEAFQQQLKATVPKKQTQRKVTELSHLSDRLQQESYILAEIPIIFQEPDNT
                     PNPKTKKNNFDFLKRSQVYNKQDNQFHKERAKAQELNLTRFKDIN"
     gene            complement(16577..19675)
                     /locus_tag="VBE34_000360"
     CDS             complement(16577..19675)
                     /locus_tag="VBE34_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7263110.1"
                     /translation="MSRMIPGRIRNQGIELYEQGLVSLISQEGNLLKAKVGDCQIEYS
                     LVTEETKCSCDFFARKGYCQHLAALEHFLKNDPEGKAILSKVQVQQESQQETKKKTSF
                     GSVFLDSLIINEDDTIKYQLSAQGEQNPYANDIWWTLKIRRLPDDRSYVIRDIKAFLN
                     TVRKEAYYQIGKQYFETLSLIQFDETSQELIEFLWRLIPSHSSKIDLEFILPNQGRHL
                     SLTRGFFEEGVTLMNALENFSFESDFHQFNHLYFKELEGEDHLYQFKVIVHRQSIELE
                     IKEKDLKPLFANSYLFYRDTFYHLNLKQEKMVTAIRSLPIEGDLAKHIHFDLDDQDKL
                     AAHLLDFKEIGLVDAPRSFSIHDFKVNFEFDINSQNEILLQMVFDYGNDLTVHNRQEL
                     EQLTFASHFKHEEKVFKLLEKYGFAPHFSTSHPAYSAQELYDFYTYMLPQFKKMGTVS
                     LSAKLESYRLIERPQIDIEAKGSLLDISFDFSDLLENDVDQALVALFDNNPYFVNKSG
                     QLVIFDEETKKVSATLQGLRARRAKNGHIELDNIAAFQLSELFANQDNVSFSQHFYQL
                     IEDLRHPEKFKIPGLSVSASLRDYQLTGVRWLSMLDHYGFAGILADDMGLGKTLQTIS
                     FLSTKLTRDSRVLILSPSSLIYNWQDEFHKFAPDVDVAVAYGSKIRRDEIIAERHQVI
                     ITSYSSFRQDFETYSEGNYDYLILDEAQVMKNAQTKIAHSLRSFEVKNCFALSGTPIE
                     NKLLEIWSIFQIILPGLLPGKKEFLKLNPKQVARYIKPFVMRRRKEEVLPELPDLIEM
                     NYPNEMTDSQKVIYLAQLRQIQESIQHSSDADLNRRKIEILSGITRLRQICDTPRLFM
                     DYDGESGKLESLRQLLTQIKENGHRALIFSQFRGMLDIAEREMVAMGLTTYKITGSTP
                     ANERHEMTRAFNAGSKDAFLISLKAGGVGLNLTGADTVVLIDLWWNPAVEMQAISRAH
                     RLGQKENVEVYRLITRGTIEEKILEMQETKKHLVTTVLDGNETHASMSVDDIREILGV
                     SK"
     gene            complement(19830..21140)
                     /gene="der"
                     /locus_tag="VBE34_000361"
     CDS             complement(19830..21140)
                     /gene="der"
                     /locus_tag="VBE34_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEO7263111.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYT
                     TGEWLNRKFSLIDTGGIDDVDAPFMEQIKHQADIAMTEADVIVFVVSGKEGVTDADEY
                     VSRILYKTNKPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPLSSVHGIGTGDILDAIV
                     ENLPVEEENENPDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTNFV
                     DSQGQEYTMIDTAGMRKSGKVYENTEKYSVMRSMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHETGKGIIIVVNKWDTIEKDNHTVSQWEADIRDNFQFLSYAPIIFVSAETKQ
                     RLHKLPDMIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYLRFLENQIREAFVFEGTPINLIARKRK"
     gene            complement(21188..22090)
                     /gene="dnaI"
                     /locus_tag="VBE34_000362"
     CDS             complement(21188..22090)
                     /gene="dnaI"
                     /locus_tag="VBE34_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEO7263112.1"
                     /translation="MKSVGQALENQGRVPRNTNDELIQMILADAQVAEFIKTHQLSQR
                     EINISMSKFNQFLIERQKFKNKDSQYIAKGYEPILVMNEGYADVSYLETRELIEAQKK
                     QAISDRINLVNLPKSYRNIRMTDFDINNESRMKAMSQLLDFVETYPSYNHKGLYLYGD
                     MGVGKSYLMAAMARELSERKGVSTTLLHFPSFAIDVKNAISSGTVKDEIDAVKSVPIL
                     ILDDIGAEQATSWVRDEILQVILQHRMLEELPTFFTSNYSFNDLERKWANIKGSDETW
                     QAKRVMERVRYLAIEFHLEGPNRR"
     gene            complement(22087..23262)
                     /locus_tag="VBE34_000363"
     CDS             complement(22087..23262)
                     /locus_tag="VBE34_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEO7263113.1"
                     /translation="MSPIDEFTYIKQNKIVYDSNSLIQLYFPIMGSDAMALYDYFVHF
                     FDDGIRRHKFSEVLNHLQYGMPRFQDALVMLTALDLLTVYQATGTYLVKLNQAMSNEL
                     FLSNPIYRRLLEKRIGEVAVAELDMKIPKNARDISKKFTDVFSDLGQPKQEVNRSKNV
                     FDLESFKRLMMRDGLRFNNEKDDVLGIYSVSELYHLNWYDTYQLAKQTAINGMIAPQR
                     MKLQQNEGQHIKDNQSFTNNEKVILRESKNDSALVFLEKIKRSRKAVTTSGEKTLLED
                     LAKMNFLDEVINVMVLYTLNKTKSANLNKAYIMKVANDFAFQNVMTAEDAVLKIRDFS
                     DQKVRTKTETKKKQSNVPEWSNPDYKDEVSPEKEIELEQFKTDALKRLERLGKDGES"
     gene            complement(23262..23741)
                     /gene="nrdR"
                     /locus_tag="VBE34_000364"
     CDS             complement(23262..23741)
                     /gene="nrdR"
                     /locus_tag="VBE34_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEO7263114.1"
                     /translation="MRCPKCGYNKSSVVDSRQAEEGTTIRRRRECEKCGNRFTTFERL
                     EELPLLVIKKDGTREQFSRDKILNGIIQSAQKRPVSSEDIENCILRIERKIRSEYEDE
                     VSSITIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITKRVRSKKSGSV"
     gene            complement(23890..25395)
                     /locus_tag="VBE34_000365"
     CDS             complement(23890..25395)
                     /locus_tag="VBE34_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEO7263115.1"
                     /translation="MKNKKDQFIGVKQPLSKKLSQLVFILFFSLFTVFSVLVYTSATR
                     YVLHREKINVGRSLEKTRVRLSQANSSLTSDDILEILYNQVFADDIYPHKRQNGIVRT
                     GESIDSILYVNQEMTLYDVNRKPVFSTLRTGMPTIGKSMGKVIISKVADMEGFVGTKA
                     IYSQKTGQLLGYVQIFYNLGRYYSMRQNIIVFLIMMEVLGTVLALVVINSATKRIVRP
                     VKNLHDLMHQISENPSNLEIRSKVRSEDEIGELSRIFDGMLDQLEDYTRRQSQFISDV
                     SHELRTPVAVVKGHIGLLQRWGKDDPEILEESLAAAYHEADRMSLMINDMLNMIRVQG
                     SLELHQDEVTDLSSSISVVIENFRILREDFQFIFENNISDIVWGKIYKIHFEQALMIL
                     IDNAIKYSPSYKEVSVVLSVDNDFATVVVKDKGEGISDEDIEFIFDRFYRTDKSRNRE
                     STQAGLGIGLSVFKQIMDAYHLKVDIKSELNQGTEFIVRIPIKKFEETKEI"
     gene            complement(25385..26074)
                     /locus_tag="VBE34_000366"
     CDS             complement(25385..26074)
                     /locus_tag="VBE34_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7263116.1"
                     /translation="MGKKILIIEDEKNLARFVSLELLHEGYDVVVETNGREGLDTALE
                     KDFDLILLDLMLPEMDGFEITRRLQAEKTTYIMMMTARDSVMDIVAGLDRGADDYIVK
                     PFAIEELLARVRAIFRRQEIETKTKEKGDSGSFRDLSLNTHNRSAMRGDEEISLTKRE
                     FDLLNVLMTNMNRVMTREELLEHVWKYDVAAETNVVDVYIRYLRGKIDIPGRESYIQT
                     VRGMGYVIREK"
     gene            complement(26309..26842)
                     /locus_tag="VBE34_000367"
     CDS             complement(26309..26842)
                     /locus_tag="VBE34_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEO7263117.1"
                     /translation="MLLTEIKKSPEGLYFDKKIDIKESLMERHSEIMDISDIQVSGHV
                     VYEDGLYLLDYNMAYDITLPSSRSMKPVVLSEKQTINEVFIEAENVSTKKELVDQELV
                     LILEEDDINLEESVIDNILLNIPLRVLAADEVGVEADLSGKNWSLMTEKQYEEKQAKE
                     KEKSNPFAALEGMFDSD"
     gene            complement(26947..27837)
                     /gene="htpX"
                     /locus_tag="VBE34_000368"
     CDS             complement(26947..27837)
                     /gene="htpX"
                     /locus_tag="VBE34_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEO7263118.1"
                     /translation="MLYQQIASNKRKTVVLLIVFFCLLAAIGAAVGYLVLGSYQFGLV
                     LALIIGVIYAVSMIFQSTNVVMSMNNAREVTEDEAPNYFHIVEDMAMIAQIPMPRVFI
                     VEDDSLNAFATGSKPENAAVAATTGLLAVMNREELEGVIGHEVSHIRNYDIRISTIAV
                     ALASAVTLISSIGSRMLFYGGGRRRDDDREDGGNILVLIFSILSLILAPLAASLVQLA
                     ISRQREYLADASSVELTRNPQGMISALEKLDRSEPMGHPVDDASAALYINDPTKKEGL
                     KSLFYTHPPIADRIERLRHM"
     gene            complement(27858..28412)
                     /locus_tag="VBE34_000369"
     CDS             complement(27858..28412)
                     /locus_tag="VBE34_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEO7263119.1"
                     /translation="MGTMILIAIIALFVIWLIVAYNSLVRSRMHTKESWSQIDVQLKR
                     RNDLIPNLIETVKGYAAYEGKTLEKIAELRAQVAKANTPAEAMTASNELTRQLSSILA
                     VAENYPDLKANNSFVKLQEELTNTENKISYSRQLYNTTTSNYNVKLETFPSNIVGKLF
                     GFKPSQFLETPEEEKEVPKVSFDF"
     gene            28540..29253
                     /gene="rsmG"
                     /locus_tag="VBE34_000370"
     CDS             28540..29253
                     /gene="rsmG"
                     /locus_tag="VBE34_000370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEO7263120.1"
                     /translation="MTPQAFYQVLIEHGITLTDKQKKQFETYFRLLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGYIDNSPLSILDIGAGAGFPSIPMKILYPEIDITIIDS
                     LNKRINFLNILANELELSGVHFFHGRAEDFGQDKVFRAKFDIVTARAVARMQVLAELT
                     IPFLKVNGRLIALKAAAAEEELISAEKALKTLFSQVTVNKNYKLPNGDDRNITIVSKK
                     KETPNKYPRKAGTPNKKPL"
     gene            29268..30647
                     /locus_tag="VBE34_000371"
     CDS             29268..30647
                     /locus_tag="VBE34_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO7263121.1"
                     /translation="MKHFFDYKTMSVARKLSISFIAVILLGSILLSLPIFQYANAPKT
                     HYIDHLFTTVSMVCVTGLSVFPISKVYNGWGQIVAILLMQTGGLGLVTLMSLSYYTLR
                     RKMSLNDQTLLQSAITYNSSTDLKKYLYMIFKVTLTLEVLAASILAIDFIPRFGLSHG
                     IFNSIFLAVSAFCNAGFDNLGATSLAQFKLNPLVNIIVCFLIISGGLGFAVWKDLIEA
                     TIQTSHKGPKLIKTFPKRLSNHSKLVLKTTTIILLTGTLLSWLLEFGNFRTIANLSLP
                     KQLMVSFFQTVTMRTAGFSTIDYTQTDFATNLVYIIQMLIGGAPGGTAGGFKVTVIAI
                     LLLLFKAELSGQSQVTFHYRTIPSSIIKQTLSILTFFFIILISGYLLLLELNPHIDPF
                     SLFFEASSALATVGVTMNTTNQLTLGGRIVIMFLMFIGRVGPITVLLSILQKKEKEIH
                     YAETEIILG"
     gene            30660..31331
                     /locus_tag="VBE34_000372"
     CDS             30660..31331
                     /locus_tag="VBE34_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEO7263122.1"
                     /translation="MKTKIIGVLGLGIFGQTLAQELSNFEQDVIAIDSNPENVQAVAE
                     VVTKAAIGDITDLAFLKHIGISDCDTVIIATGNSLESSVLAVMHCKKLGVPQVIAKAR
                     NLVYEEVLYEIGADLVISPERESGQNVAANLMRNKITDVFQIESDISVIEFKIPKSWV
                     GKTVEQLNIRHKFDLNLIGIRKAKNKPVDTEVPINTPLEEGIILVAIANSDAFQRYDY
                     LGYFN"
     gene            complement(31366..32196)
                     /locus_tag="VBE34_000373"
     CDS             complement(31366..32196)
                     /locus_tag="VBE34_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO7263123.1"
                     /translation="MAKQLIGYQEGYGFLYRLSGASKLLFFILVSVACMTTYDTRFLI
                     VVSLGSLVLFRLAKIKWQQVSFVMTLVVVFAVLNIIMVYLFAPHYGDKIYGSSSLLLK
                     GIGPYDVTSQELFYLFNLILKYFCTVPLALLFLMTTNPSQFASSLNQLGLSYKIAYAV
                     SLTLRYIPDVQEEFYTIRRAQEARGIELSKKSNLVARIKGNLQIVTPLIFSSLERIDT
                     VATAMELRRFGKNKRRTWYSKQSLEKSDIVLIILALASLFVSLYLIHLNHGRFYNPWQ
                     "
     gene            complement(32189..33865)
                     /locus_tag="VBE34_000374"
     CDS             complement(32189..33865)
                     /locus_tag="VBE34_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7263124.1"
                     /translation="MKDFIEWKDFTFQYDVQSEPTLKGINLSIPKGEKVLILGPSGSG
                     KSTLGHCLNGIIPNTHKGQYSGIFTINHKNAFDLSIYDKSHLVSTVLQDPDGQFIGLT
                     VAEDIAFALENDVVAQEEMASIVEMWAKRLEIAPLLSKRPQDLSGGQKQRVSLAGVLV
                     DDSPILLFDEPLANLDPQSGQDIMALVDRIHQEQDATTIIIEHRLEDVLYERVDRVVL
                     FSDGQIIYNGEPDQLLKTNFLSEYGIREPLYISALKNLGYDFEKQNTMTSIDDFDFSE
                     LLIPKMRALDLDKHTDKLLSVQHLSVSYDLENNTLDDVSFDLYKGQRLAIVGKNGAGK
                     STLAKALCQFIPNNATLIYNNEDVSQDSIKERAERIGYVLQNPNQMISQAMVFDEVAL
                     GLRLRGFSDNDIESRVYDILKVCGLYQFRNWPISALSFGQKKRVTIASILILNPEVII
                     LDEPTAGQDMKHYTEMMSFLDKLSCDGHTIVMITHDMQLMLEYTDRAIVIDNGLIVAD
                     DHPINILSNTELLKKTHLKKTSLFALADRLGISPQKLTQWYIDNQGGKNG"
     gene            complement(33882..34430)
                     /locus_tag="VBE34_000375"
     CDS             complement(33882..34430)
                     /locus_tag="VBE34_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEO7263125.1"
                     /translation="MNTNTIKKVVATGIGAALFIIIGMLVNIPTPIPNTNIQLQYAVL
                     ALFAVIYGPGVGFFTGFIGHALKDSIQYGSPWWTWVLVSGLLGLMIGFFAKKLAIQLS
                     GMIKKDLLLFNVVQVIANLIGWSVVAPYGDIFFYSEPASKVFAQGFLSSLVNSITIGV
                     GGTLLLLAYAKSRPQKGSLSKD"
     gene            complement(34599..35807)
                     /gene="sstT"
                     /locus_tag="VBE34_000376"
     CDS             complement(34599..35807)
                     /gene="sstT"
                     /locus_tag="VBE34_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEO7263126.1"
                     /translation="MKRFLKAWRKTSLIKKITIGVVIGLFLGILVPKASAIGLLGQLF
                     VGGLKAIAPLLVFTLVISALSQHREGGKTNMSTIIGLYITATFAAALIAVVVNYIFPL
                     TLILKTPAKTDLLPPKGISEVFQSLLLKIVDNPIHAITEANYMSILFWAVIFGLAMRS
                     SNQRTKDLMQTFADATSQVVKWIINLAPIGIMGLVFTSISENGIGILGDYGLLILVLV
                     GTMLFVALVVNPIIAFVMMRKNPYPLVLRCLKDSGITAFFTRSSAANIPVNMRLCEDL
                     GLDKDTYSVSIPLGAAINMAGAAITINILTLAAVNTLGITVDFPTAFLLSVVAAVSAC
                     GASGVTGGSLLLIPVACSLFGISNDVAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     VAEKSVFGKK"
     gene            35983..37350
                     /gene="brnQ"
                     /locus_tag="VBE34_000377"
     CDS             35983..37350
                     /gene="brnQ"
                     /locus_tag="VBE34_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO7263127.1"
                     /translation="MKLKNTYIIIGLMLFALFFGAGNLIYPAYLGIYSGSNWAWAIIG
                     FCLTGVTLPLLGVAAVTYSGATDVESFTKPVSKTYAIFFAVALYLSIGPFFAIPRTGA
                     TSFSVGIQPILGDSSLVKIIYGLIFFGISYFLAIRPSQIVDRIGKYLTPFLLTVISIL
                     IIASLIHPAAIVGTPHNASTNVSDAFKDLPFLAGLIQGYGTMDALASIAFAIIVIQAS
                     KQYGAITKKEITSMTLKSGAIATFLLAFIYIFVGRIGATSQSLFKFANGSFLLHNTPI
                     DGGHVLSQSANFYLGIVGQAILGTAIFLACLTTATGLITACAEYFHKLLPKISHITWA
                     TIFTLIAITFYFGGLSEIIRWSLPVLYLLYPLTIVLIFLVFFDQKFESSRIVYQTSIA
                     ATAVAALYDALSKLGEMTGLFTIPSALTTFFTKVVPLGEYSMGWISFAICGVLVGLIL
                     KKVKA"
     gene            37464..38519
                     /locus_tag="VBE34_000378"
     CDS             37464..38519
                     /locus_tag="VBE34_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO7263128.1"
                     /translation="MRAATYLSANELSLTDKAKPQVIKPTDAVVRLVKTTICGTDLHI
                     LGGDVPACKPGTTLGHEGIGIVEEVGSAVNHFKVGDKVIISCVTACNTCYYCKHQLPS
                     HCEDGGWILGHLIEGTQAEYVHIPHADGSLYHAPEGVCDDALVMLSDILPTSYEIGVL
                     PSHIKPGDTVCIVGAGPIGLSALLTAQFYSPAKIIMVDLSQKRLEASKKFGATHTILS
                     TSTQEVKEEIDKITKGRGVDVVLECVGYPATFDICQNVVSIGGHIANVGVHGKPVEFN
                     LQDLWIKNITLNTGLVNANTTEMLLEVLETGKIDATQLVTHHFKLSEIEEAYKVFKAA
                     EENNTLKVIIENDITPA"
     gene            complement(38568..39260)
                     /locus_tag="VBE34_000379"
     CDS             complement(38568..39260)
                     /locus_tag="VBE34_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000574038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7263129.1"
                     /translation="MIEWIQTHLPNVYQMGWEGAYGWQTAIVQTLYMTFWSFLIGGLM
                     GLLGGLFLVLTSPRGVIANKLVFGVLDKVVSVFRALPFIILLALIAPVTRVIVGTTLG
                     SPAALVPLSLAVFPFFARQVQVVLAELDGGVIEAAQASGGTLWDIIVVYLREGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGSVAITKGYNYSRDDITLVATILILLLIFFIQF
                     LGDFLTRRLSHK"
     gene            complement(39257..40327)
                     /locus_tag="VBE34_000380"
     CDS             complement(39257..40327)
                     /locus_tag="VBE34_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEO7263130.1"
                     /translation="MSKEPIIKLDNIDVTFHQKKREINAVKDVTIHINQGDIYGIVGY
                     SGAGKSTLVRVINLLQEPSAGKITIDDQVIYDNKVTLTSTQLREQRREIGMIFQHFNL
                     MSQLTAEQNVAFALKHSGLSKEAKAAKVAKLLELVGLSDRAQNYPSQLSGGQKQRVAI
                     ARALANDPKILISDESTSALDPKTTKQILALLQDLNKKLGLTIVLITHEMQIVKDIAN
                     RVAVMQNGKLIEEGSVLDIFSHPRESLTQDFIKIATGIDEAMLKIEQQEVVKNLPVGS
                     KLVQLKYAGHSTDEPLLNQIYKEFEVTANILYGNIEILDGIPVGEMVVILSGDEEKLR
                     QACQAITDSQVQLTLLKEGGKA"
     gene            complement(40320..41696)
                     /locus_tag="VBE34_000381"
     CDS             complement(40320..41696)
                     /locus_tag="VBE34_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEO7263131.1"
                     /translation="MTLTNEENQIQKFLSDTIVQDDINILRDLIAIKSIFAQKVGLND
                     LSSYLGEVFRKAGAEVIIDDSYSAPFIVANFKSSKVDAKRIIFYNHYDTVPADEVEQW
                     TEDPFTLSLRYGKMYGRGVDDDKGHITARLSAVKKYLSRHKGELPLDITFIVEGAEES
                     ASVGLDYYLEKYQEQLQGADLIVWEDGPKNPKGQLEIAGGNKGIVTFDLSVSSADVDI
                     HSSFGGVVDSSTWYLIQALNTLRDNKGHILVEGIYDKVIPPTKRELELVEKYSYRSAK
                     ALEGAYQLVLPSLADSHKTFLRKLYFEPSIAIEGITSGYQGEGVKTILPAYAKCKAEV
                     RLVPGLTPKGVLDSIQNHLKENGFKDIELTYTLGEMSYRSDMSAPSILKVVDLAEQFY
                     PEGISLLPTSPGTGPMYLVHQALRAPIAAIGIGHANSRDHGVDENVSIADYYTHIELV
                     EALIESYE"
     gene            complement(41830..42654)
                     /locus_tag="VBE34_000382"
     CDS             complement(41830..42654)
                     /locus_tag="VBE34_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO7263132.1"
                     /translation="MKLKHIVLGLALTTLLGVTFSNQEVSASSTSSKVVKVGVMTFSD
                     TEKARWDKIEKLVGDKAKIKFTEFTDYTQPNQATANKDVDINAFQHYNFLENWNKENK
                     KNLIPLEKTYLAPIRIYSEKVKSLKKLKKGATIAIPNDATNGSRALYVLQSAGLIKLN
                     VSGKKVATVANITSNKKDINIQELDASQTPRALKDVDAAIINNTYIEQANLKPSDAIF
                     VEKSDKNSKQWINIIAGRKNWKKQKNAKAIQAILDAYHTDEVKKVIKDTSADIPQW"
     gene            complement(42795..43628)
                     /locus_tag="VBE34_000383"
     CDS             complement(42795..43628)
                     /locus_tag="VBE34_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7263133.1"
                     /translation="MTIKKVLSVTGIILVTVASLAACSSKSHTTKTGKKEVNFATVGT
                     TAPFSYVKDGKLTGFDIEVAKAVFKGSDNYKVTFKKTEWSSVFTGIDSGKFQMGGNNI
                     SYSSERSQKYLFSYPIGSTPSVLAVPKNSNIKAYNDISGHKTQVVQGTTTAKQLENFN
                     KEHQKNPVTLKYTNENITQILTNLSDGKADFKLFDGPTVNAIIKNQGLTNLKTIPLTM
                     RDQPYIYFIFGQDQKDLQKYVNNRLKQLRKDGTLSKIAKEYLGGDYVPNEKDLVTPKE
                     K"
     gene            complement(43730..44419)
                     /locus_tag="VBE34_000384"
     CDS             complement(43730..44419)
                     /locus_tag="VBE34_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEO7263134.1"
                     /translation="MLTKPIIGITGNEREMSDIPGYYYDSVSRHISEGVKNAGGLPVI
                     LPISEAESAKAYVEMIDKLIISGGQNVLPSYYGEEKIIESDDYSLARDIFEFALVEEA
                     LKQNKPIFAICRGMQLVNVALGGTLNQSIDNHYQEPYIGFAHYLNVEKGSFLEGFISG
                     DFKINSLHRQSVKLLAEGLIVSARDPRDGTVEAYESRTEQCIIGVQWHPELMLHQIEN
                     QTLFGYFVNET"
     gene            complement(44574..45290)
                     /locus_tag="VBE34_000385"
     CDS             complement(44574..45290)
                     /locus_tag="VBE34_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7263135.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKQGEPDPESN
                     SALKFVLDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADTVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            complement(45716..46420)
                     /locus_tag="VBE34_000386"
     CDS             complement(45716..46420)
                     /locus_tag="VBE34_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263136.1"
                     /translation="MRLVWKFLSEKLVCKIMDSSYGVLILFFICLSITAVGGYTLVYN
                     LSGYSSMDAQKVFGIIVLTIILLVFIVGTIFYAIGSAQLIMSILSKKKISFDEKDAPL
                     DGATHVGETVFQGKEVSLYSKQDKFFLWSRKWQKGFSKYQYYHIRPVDQTEISEVYQV
                     ERCCFYCDHIFTQFFIREGKNREKIVELCTIDVQIGNDRDLGFKQVNRGEFEKTVPFK
                     DVEIKEIKKLIDLKQI"
     gene            complement(47081..48034)
                     /locus_tag="VBE34_000387"
     CDS             complement(47081..48034)
                     /locus_tag="VBE34_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEO7263137.1"
                     /translation="MKICILGLGVIGTTYGYIFQKAGHQVEHLVRESKKQDVPLKLDI
                     TMLDGRYNNKGEEKTDIYTVNLAKPNTNYDFILLSVAGGRIKDAIATLSQNNITGSLL
                     LFCNFWNSREEIEKMVGDFKYIIGFPTAGGNLKQKKLNCVLFDHIMLESEEKATITNY
                     KELLEFLESADIKVENPYDMIEWIWIHMAINAGVTSTAARAGKIDDPKQLAVNLMRDN
                     HGLSIAVKSIRETIKVVKNRGVDLRLYKNELFPYKIPSTIAGIIMKRMFARNELTSRI
                     MTLHNDVKDIMYGCKCIYDAGKQEKLNLPLFYHNMDKIFNS"
BASE COUNT        15280 a         9549 c         7349 g        16182 t
ORIGIN      
        1 tacaatgcca gtttttcaga atttacatgt ccaccaatta ttttataaaa ttgctttaaa
       61 tcatagtgat aggacttacg cgaattgaga gataatttct tactctcaat aaaattatta
      121 atatcattta tcattgtttt taggagtgat agtatattcg tctgtaaaat catgtaggag
      181 gctattaata gcttttaaaa tcgatttacg ggttataatg cctctaaaat cattttgatc
      241 actgataaca ggtaaaaatg gataatctac aatcttatgc ataattgctg tcagagtagc
      301 tgcctcatta atcggctcga tttttgtatt aaccatctca acaatatcag tctgtgccat
      361 ctcccaatct gttaattgac ctttagattg gtagcccata atgtctgaga tactaatagt
      421 acctacatat tttttctctt tagtgataac tggtacacgt gaaaaaccat tacttgctaa
      481 tagtaacata acgtggtctg cattgtgtgt atccacaaaa atagcaacgt cttcagctgg
      541 aattagatag tggtccaaat gatttagcaa aaacgactca aattctttag caatcatctt
      601 gaaaattcct ttgtcaagtt aggatagggt tggtgttggc gagtataata ctctaccaaa
      661 accttactat ctgttatagt tacaacagcg taaagcttct catttattgg tcctcttggc
      721 tgcaaaacac tacctggatt gataaaaata gttttaccat ttttccaagc atctgcacga
      781 tgaagatgac catataaaca aatatccgcg tcttcttgct gagctaataa atccaattta
      841 tcccaggtaa aattaatctg atggagatga ccatgggttt gaacaataac agcattatct
      901 atttttgtaa ccaaaacttc cggataacct gaatcataat cacaatttcc tgtgaccaca
      961 tgaatgcctt cccatatagg atctgagcta ggaagttcgg aatccccatt gtgaaaaata
     1021 gcatccactt ttcccaaata atgattcttt atatctttta caatgtcacg atctccgtga
     1081 gaatcactca ttacaactaa ttttctaatt gccattttgg aaatacctcc atcaatttac
     1141 ggacagcttg cccacgatgt gatagattat ttttttcttg tgcgctcaat tcagctgctg
     1201 tcctactacc ttctccgaca ataaagagcg gatcataacc aaagccattt tcccccttag
     1261 gttccgtacc aatataacct ggccactctg cttcaactac caaactttct ttatttggag
     1321 ctgacacgac taaggttgta tgaaactgtg ctgaacgtct ttctttgtca aataccatgg
     1381 ctaattcatg caataatttg gcattattac gagcatctgt cgcgtcagga cctgaaaaac
     1441 gagcagacca tacccctggc aaaccaccta aagcatctac ttttaatccg gaatcatctg
     1501 aaattaccat ttttccagtt aattttgaaa tagtctctgc tttaagtcga gcattttctt
     1561 caaaggtcat accagtctct tcaacttctg gtaaatctgg gtaatcattt aaattttcaa
     1621 ctttgagtcc taatttccca aataattctc taaattcctt cgtttttcct tcattgtgag
     1681 tcgcaattaa tatggtatcc ccaaaattat tatcattctt taaatcaaag aatttttcta
     1741 aagaagtgcc ctctttaggt aagtgaacta agaacaactg atcatcttca ctaactaaga
     1801 tggccccttt atgttgagtc actttgatat gccttgaata ctcttgatta atcatatcaa
     1861 cgaggaatgc taataattga aaatccgaag tcatctgcac aacgtgaagc tgtagacctt
     1921 caactttcaa agttgataat agacgatgaa aaacatcttg gatagcttcg taagattcat
     1981 cattaccaaa acaagtaaaa taattaaaag cttggaaact acctaaaaac caattgcctt
     2041 cagttttact ttcaaaaatt gtttttgtca taatacgata cgctccacat ttatttcttg
     2101 actaagccat tgttctgcaa tttctttaaa accttttggg ctggcggttg tgtaaaagtg
     2161 atgaccaccg tgtttatttt gccaattatg gtttatctca aaatagttca ataaaacaga
     2221 aatatcacga acggtttctg cgccactatc aattaattta acctcagccc ccataacatt
     2281 ttgaatgatg ggacgtaata ggggataatg cgtgcaacct aaaattaaag tatctaattt
     2341 accaactaat ggggacaacg tttcataaac cacctttttg gctaaactag aagacatctg
     2401 atttgattcc acaattggaa caaatttcgg acaagcaagg gataccacag cagtatttgg
     2461 agacaaagct tgaatttttt gacgataagc atctgattta acagtcatgg gagtacctat
     2521 aataccaact ttccctaaat tagttgattt gatagctgcg ctagctcctg gtaaaataac
     2581 gcctaaaaca gggatgtcta gtttttcttt aatttcttgc caggcaactg cagttgctgt
     2641 attacaagct ataacaatca tcttaacatt tttagtcaat aagaagttaa ccatctgcca
     2701 ggtaaactct ctaatctgtt gagcaggtct aggaccatac ggagctctag cctgatctcc
     2761 aatgaagatt acttcctctt ctggaagttg acggaacatt tccttaacaa ccgttaaacc
     2821 acctacacca gaatctagaa agccaatagg cctactatcc atttttattc ctccaatatt
     2881 agtgactaaa agaggctgga caacagtcct agcctctcta tacaataacc aatcagtcaa
     2941 atctacactt gaccaagttt gcccaaatct tgtctagaaa cctgatggac tatcactcac
     3001 tatttcatca ctaaacgctt caatgctaaa acactcttat tttttagttt tgatagctgt
     3061 ttttgcgtgt tttacaatat tacggtatgt ttgttgaact ttagcttcac taggtttttg
     3121 tcccatttga ctcatcattt cacgaatagc atccggtgtt aaacgagggt gctctccaat
     3181 ctctttttca atttgtttac gagcaataaa aatgcctccc acgagaccac caaacaaagc
     3241 gacgataatt aataaaatcc aaatagttgt tgacatctat tcattctcct catttattta
     3301 catgactcat tatagcaaaa aaagaaagag tttacaaata tatgtaaaca aaatcaaagc
     3361 ctctatcgtc cttgtcacgt caatacatta ttaaattttc actagagcat ggttaagcac
     3421 atagtcgagt cctttatctt atcctaagtg ttttccccaa cttttgtgtc atccatttcc
     3481 aaatgaagag agctatgtaa aaatctacca atccatgtag caaagagcct agtcctatca
     3541 aagaaaatag tgaccataaa taagttgaac tactgttaga agtcattgtg attatataaa
     3601 cagtaataaa ttcgagcaga ccatgaatta tgttaatagc tacagcaaat aacaatgttt
     3661 gccatttact catcaataaa gagggctttg acttaatcaa gaaagccgca attaatgcaa
     3721 atccgatatg tgataaagct ctaagaacga ttacaatcgg aaaaccagct agtagaaatc
     3781 caagtcctgt ccctaaagct accaatattg caactggaac agatataaaa atacttaaaa
     3841 ataaaggtac atggcttgct aaggtgaaag aagcaggccc aataattatt ttagcaggca
     3901 tgattatagg aatcatgatt gcaaaagcta ctaaaataga agtataagaa accatttgtg
     3961 ttgttttttt tgacattttt atctcctttg aagctattat acaaaataac cttacaggtg
     4021 taaagacacc tgtaaggtta tttgtctatg tataaaattc ctgctttttt tagagcctca
     4081 catatttggt caaaaactga ctgatcttga caagaaatca tatgtgtatg tagaccatct
     4141 gtcaaagagg atagcaattc tgcattggaa acttttaatt tagcagtaaa attatcaatg
     4201 tctttttggc tttgaatgtt taagggagca gtcagcatcc cataaatagg gtgctctacc
     4261 tccacattca taatgattcc ttgataccgc aaaataattt ccagttcttc ttctgtctgt
     4321 tctatgccat gctgacaaac caatctagct gtgaattgat gagtacttag agcactggac
     4381 ataaggtatc cttttggggt agaaataata tcacattgtt gtgcacgcaa taaggcaata
     4441 tctccaacaa tcacctgcct actaacacta aaaatctttg ctaatgtgct tgccgatata
     4501 gcttcttttg ttccttttaa agtcgttaaa atattttctc gacgctcctg tgccttcata
     4561 taacaaattc cttctttggt gttatttcac aattaaaaac tggctagaat atctaaccag
     4621 ttagcctcaa tcatttcgtg caaaaagacg aagaatattt aagaatagat taataaaatc
     4681 aagataaagg cttagtgcca tcgaaactgc ccaaccatct gcgacttggc cgccttggct
     4741 attgtagaca tatttaatca tttggttatc gtaagcaatc aatccagaaa agatgatgac
     4801 acatacgata ctaatgatat aactcatacc cccactaccg ataaataaat taacaagact
     4861 tgcaattaaa atcccaatta aagcagccat taacgcttta cgcatacctg ataaatcttt
     4921 tttagtctta gctccaatca aagccattgc aaagaaaaca gccgcagatg ttataaaagc
     4981 ttgaagaact gtagtttgcg tgtaccttgc gataatgaaa cttaaagtaa acccattcaa
     5041 agctgaataa atcaaaaata atggtaatgc agctggtgtg tttcgacgcg ccgctccact
     5101 agcaacaaag actaaaacca attctaagat gattgctcca aaatacaacc ctggcatttt
     5161 ttgcatgatg gcaatcatat tttgtggaaa catatacagc ataatcgcag aaacagcagc
     5221 tgacaaacct actcctatcc ctacaagacc gtaaatctta gcaaaaaatt gattcagccc
     5281 tgaatcagat tgtgtgtaaa tgacgttatc attcatatgt attttcctat tctaaatttt
     5341 aatgattatg actacctaag aatttgcgac taaaacggat aggtttctta aaaatacgtt
     5401 tgagtggacg agaagcctct cgtactgccc aatacttatc aaccatagtc acaatataac
     5461 tttctttata acgtggcggt gcaatagtcg ctccccacat gtgcttcaaa ataatatctt
     5521 cttctctagc gtttaaatca gttaattttc gcgcattacg aaccgctatc cttggatgaa
     5581 cccaggcatg gctcttgtta aatttcgtta cacgccaatc ataataaaag aaatcatgta
     5641 gtaaaccacc tcgagcagta ctactcgcat cccaaccaaa cttcttagca attttataac
     5701 ttgtataact aacatttata gaatgctcta agcgagttga gtgatgatgt tggacaatag
     5761 cttcgagttt ttggaaacgt ggatggtcaa taaggtgacc gaccaaatcc atgaagtcct
     5821 tatcgtcttg atactttttc attataatca cccctctggg gatattataa catatgaact
     5881 taaattaagc tgaaaatgac tactcccgca gctactgcta cattaagact ttctgcttgt
     5941 ccaggcattt caatatgtgc taaaacatct gcttcttctg tcatagtttt ggaaattccc
     6001 tgtccctcat ttcccatgat taaagcaaaa taatcatatt tagggagatt cttataattt
     6061 accgattgtt cagataaagt tgtcgcaatc atagttatcc cttctgcttt acaaaagtta
     6121 acaaactgct ctatttctac cctaaatact ggtaaatgaa agtgactgcc ttgcatagaa
     6181 cgaagtgttt tttggttata caaatcagca gatttctgag ataaaaaaac agcatcaaaa
     6241 ttcgctgcat ctgcagttcg aaccattgtt cctaggtttc ctggatcctg cacatcttct
     6301 aatacaagaa aacgtccttt tttaatatct gtccatcttg tctcttgaaa ggcaatctca
     6361 gctacaatcc cttgaggagt ctgtgagtca gttaattctt tcaaaacatc atctgtaaca
     6421 acaatagctc tctcgggttt tctcaaacga tctattgccg tttctgtcac aaaaatattt
     6481 aaaaattgtg caccatattt ttcagcttcc tcaaataaat gccagccttc aataagatat
     6541 gaagatttcc gatatttttt ttgtaacaac tttttcgttt ttttaatgag attatttgat
     6601 tttgaagtta taatagtagt cataaaccta ttatatcaga aaagaggtca agttatgaaa
     6661 aaagttcatt taattgtctc agggcgcgtt caaggagtcg gatttcgcta tgcaacttat
     6721 tccttagctt tagaaatagg tgatatttac ggtcgcgttt ggaataacga tgatggaact
     6781 gttgaaattt tagcacaatc cacagattca aataaaatga cacaatttat tcagaaaatt
     6841 cgaaaaggac cttcaaaatg gtctaaagta acttatgttg atattaaact agataacttc
     6901 gatgatttta atgactttaa aatgagtaat taaaaatcat tctgcggaag tattgtttaa
     6961 ttcttaaaaa aaaagtaaaa tagataggta tattaaaaat tagaggaact aaaaacattg
     7021 aaaaaaacat tgaaacgtat cttattctct agcttatctt tgtctatgtt attattattg
     7081 acaggatgtg ttagcgttga taaggctgga aaaccctatg gagttatctg gaatactctt
     7141 ggagtgccta tggccaacct cattacttat ttcgctcaac accaagggct tggttttggt
     7201 gtcgctatca ttattgtaac cgtcatcgta cgtgtcgtta tcttaccact tggactttat
     7261 cagtcttgga aagctagcta tcaagctgaa aaaatggcgt actttaagcc tttatttgaa
     7321 cctattaatg aacgactacg caatgctaaa acacaagaag aaaaattagc agctcagaca
     7381 gaattaatga cagctcaacg tgagaatggc cttagcatgt ttggcggtat tggctgcttg
     7441 ccattactca ttcagatgcc atttttctca gctattttct ttgctgcacg ctatactcct
     7501 ggagtctcta gtgcaacctt cttgggactt aacttaggtc aaaagagtct gatacttact
     7561 gtcattatcg ccattcttta ttttgtacaa tcttggttgt caatgcaagg tgttccagat
     7621 gaacaacgtc agcaaatgaa aacaatgatg tatctgatgc caataatgat ggtattcatg
     7681 tctattagtt tacctgctag tgttgcgctt tattggttta ttggtggtat tttcagtatt
     7741 atccagcagc tagttacgac ttatgtactt aagcctaaac tacgccgaaa agtagaagaa
     7801 gaatatacta aaaatcctcc taaagcctac aaagctaata acgcacgaaa agatgttaca
     7861 aattcaacta aagccactga atctaatcaa gcaattatta catctaaaaa aacaaatcgt
     7921 aacgccggaa aacaaaaaag acggggataa aacaagcata ctaaataatg gtgtgttgtc
     7981 ctaaacagtt aaagattatt atgtaattca agtaaaaaag acttagttct atgtcctaca
     8041 tagctaagtc tttttagttt tgcctaactt ttcatataaa aagcacccta aaagtcagat
     8101 aaaaatctag cttttaaagt gcaagtaatc tagctaacag actaccttat taataaagta
     8161 atcgtttttc ccaccagtct tgaataattg cataaagcgt acaaattacc caatagataa
     8221 gggctactaa gatataggcg gtcatatagt cggagtaggt tcccccaact attttagcat
     8281 tattaaagat atcaggtacc gtaatcatcg cagtcaatga ggaacccttg accatatcta
     8341 gcaaaacatt acttaaagaa gggagtgcaa ttcttgttgc ttgcggaata ataatgcctc
     8401 tataaatcgt cggtgttttt aaccctaaag ctctagcagc ttcccactga ccatgatcaa
     8461 cagctaaaag agctgctctg ataatttctg atatatatgc actagacatc atggtaaaac
     8521 ctacgataga agcagtcaca gcatctaact gtatccctat aaaaggcaaa ccaaagtaga
     8581 taagaaagag tagaaccatt aaaggaatcc cacgcattaa ggaaatatga atattagcta
     8641 gatacctcag taagcggaaa tgagacatct tcattagagc tacaaagaat cctacgatag
     8701 ctcctaaaat aaagccaacc aaggataatc caatggtata tggtaacccc tgaataacac
     8761 ttggaaaagc cttaacagct agttccaaat taaaaatggc atcccagttt atcacttatc
     8821 tactcctctc gaaatttttt atttaacttt aattactgga attttatccg ttccagcagg
     8881 ttcttttgtg agatcttgac cagcataata tttttcagag atttttttca aagaaccgtc
     8941 tttcttcata tccttgatag ctgcatcaat ttttgttttg aggctcttat cttttttact
     9001 catcacaata ccttgctcag tcggattata cttcacatca cccattttta ctttgatatc
     9061 tggatattgt tttgttacat attttacagc aatgacttgt gtgtagtaat cattcggaat
     9121 gaaatctgta cgtccagttg atacatctcg aaggtaaacg tcattagtta cattgtcata
     9181 aataacaggt tcagcacctt gctgttttgc aattttcata tattgcgttc cagcgccacc
     9241 accagctttt ttacctttcc agtcagataa atcttttgct gtaatctttg aagaaccatc
     9301 tgcacgtaca atcatacctc caacagaata tttataaggt tgagagaaat tgtatttttt
     9361 caaacgctcg ggagttgtgt caaagttatt gacagcaaca tccaccttac cactatctac
     9421 agaagtaaat gattcagcaa ctccaacttc aacaaactta acttttatct taagtttttt
     9481 agcaatcgct ttcatcatat caatctcata accagtcaat tttttatggt catcataata
     9541 acttgttgga taaagcgttg caggggtagc aaccttaagg acaccatctt ttttaatatt
     9601 gtcccatgtt gtttctttgt tagattgacc atttgagcaa gctgctaatg tcattgcaga
     9661 taaaccgact aaaccaagta aaattgtctt tttcataagt actcctgtag attaaattat
     9721 attaaaatgg tttggcgtac tctatcgaaa attaaattta atgaaaatta atacataaat
     9781 ttacgataat aatacaacca ttgttaagag taccatattg tatccgtttt cgcaaaaata
     9841 ttgtttactt ttcaaattaa tggtaaacag gccctttgaa ttgcttctct ttttcaaaca
     9901 actctgcaac agcaagcaat aaatcttctc tccctttagc cgctgtcaat tgtacaccta
     9961 aaggtagccc ctctttagtt agatgagttg gtatggctaa agaaggttgc cctgtcaagt
    10021 ttggctgatg tgtaaaaggt gtccaggcca ttgagtcttc aaacattttc caaattaaat
    10081 cctgttgttc acttactgag aattctccca tatgcctcaa ttgtttctgg agtgtctcgt
    10141 ctaaatcaaa ttgtccatga aaaggagctg gttgattagt tgctgctgtt aaaattaaat
    10201 cataattttc atgaaacctc gccatggtag ctgcaaattg atcccatgaa tctaagagtt
    10261 tactgtaatc tttagccaat accctttgtc cactctgata catagcccag gtcattaatt
    10321 ccatatcgct aaattccatt gaacgcccta aactcttttc gatgtcatca aacattgctg
    10381 ctgtttcaac gctattcatc atacaataag ttttcataga atgaatacca tctaaaggtt
    10441 gttctactaa ttctaaatga ttcccttttt gagacagaaa atctgcggct tctttaatag
    10501 ctgctttagc ctcacttgat acctttgttt ttaagggaga atccatcaat actgctattt
    10561 ttaggggttt actaacgcta aactcgaaaa gactctcttt gctgagtttt ttcaggggaa
    10621 atgggctttc tacttgatat gattgtaaat aatataatag tcgttttgta tctctaactg
    10681 actttgttaa agcaaaatga ctagaagccc cttgccaacc tctataggat gaaggtccaa
    10741 ctggtatacg tcctcgactc ggttttaaac caatcaaacc attaaaactt gctggaattc
    10801 tgattgaccc tcctccatca ctagctcctg caattggaac cattcctgaa ctaacagctg
    10861 cggctgcacc accactagag cctccagcat ttcttgaatg gtcaaatgga agattaacat
    10921 tcccgtgaag ttgaccatca ctaatattct taaaaccaaa ttcaggggta tttgttcttc
    10981 ctaaaataat aaaccctaat ttttcaaagg actgcactaa atagtccgtt tgtttagcat
    11041 gatagtgttt aaataattgt gaacctgagg ttgagagttg tcctttttga ttctgtccca
    11101 aatctttcag aagaatagga actcctgcaa aaggagcatt tgattcattt gcgtatttgg
    11161 ctgcttgacg cgcctcatta tattgcttgc tgacaacagc attaacgctt acattctgtt
    11221 cctcgatctt gtagattgct tgctctacta attcttgcga cgaaatctta tgctgtttta
    11281 tcgcttgtac catagcggta gcatctttaa aaaccatata aacacctcat aaaatgtatt
    11341 tttattataa gataacaaaa aatacttaaa aaaaatagaa taacgtctag actctagata
    11401 ttattctatg atttatacta ttaatttgtt ttttcaacac taatgatttc aacttgatag
    11461 ctaccagcag gtgattcaat tgtagctaag tctcctgttt ttttaccaat taaagcatga
    11521 gcaattgggc tttcattcga aatttttcct gaaaaaatgt cagctccagc agcaccaacg
    11581 atatggtaag tatctttatc atttgtacca acttcttgga caaggacagt tttaccaatt
    11641 gcaacctcat ctttagcaac tgcatcacta tcaataattt cagcataacg aatttttgtt
    11701 tctaaaattt gaatttgacc ctcaacaaat gcttgttcgt cttttgctgc atcatattcg
    11761 ctattttctg ataagtcacc ataagaacga gcaatcttga tacgctctac aacttctggt
    11821 ctacgaacta attttaactc ttcaagttct ttttcaagtt gatctttttc cacttgtgtc
    11881 attgggtaag ttttttctgc cattttattt ctcttttctt ttcatttttt caagtcaaag
    11941 atataaacat gatgactcgc accatgttta taagcttagt taatttgact attgatatat
    12001 ttttcaacgt tagccttgtg tgtttcaaaa tctttagcgt agtatacatt tccggtcttc
    12061 acatcagcga caaaataaag gtaatctgtt gaagcaggtt ttatagtcgc ttcaattgct
    12121 gagatacttg gactatcaac tggtccaggc atcaaaccag tattagtata gatgttgtat
    12181 ggtgatttaa ttgaagtatt aatttgagca tcttcagcta aacttgtttt atcacctaat
    12241 ttccccatag catacaaaat tgcaatgtta ctttgaagag cttgaccggc tgataatcgg
    12301 ttataaaaga cacttgctat tttacggcga tcttcgtctg ttgatccctc tttttcaaca
    12361 agagatgata atgtcaaaac atcatttaca gacatattct tcgctttaat agtgttatag
    12421 taaggtgcta tcttagtatt cattgttgaa atcatatctt caacaagtcc ttctaaggtt
    12481 gtatctttat aatagttgta ggtagctggg aataaatatc cttccaattg ataaatagct
    12541 tttgatttat caggtaaact tcctagtaaa tttggatatt tagctaccat ttttttaata
    12601 aacgtttcat cttgaacaag tttcaagaaa tcatcagctt tataaggtgt acttgtagtc
    12661 tttgtatcaa ttttattaga ctcaatagct ttagcaatct gtttaatagt gtaaccctcg
    12721 gtaattaata tcttacctaa agctggtttt gtcggttctg cagttccgcc tttttccaat
    12781 tcagcagcaa tctgatctaa agtcatactt tttttaagat tgtagtatcc actttgaaaa
    12841 ttactatagt ttttgaactt ggaatagtaa ttaaagactg tggcgctctt aatgactcct
    12901 gctttttcta aaatttgacc aattaattta ttcccagatc ctatcggaat ttcaacttgt
    12961 acaaatttat ttgaattgga atcaacaggt ttaatagcac tatctacaaa acgataacct
    13021 acaaatgctg tcaagagcaa tacaagtatg ataattgata tcagaatacg tgcaattcgt
    13081 tttgccacat tatctgcttt ctgacgtctt tcacgcttgt tatgtttgtt cttggctgac
    13141 aaagctatac cctcttcttc ttttgacaaa tcctctactc ggataggttc ctttgctacg
    13201 ggctctaact ctgatgtctc accaagtttt acattttcac cttctgtagg aatatctgtt
    13261 gctctcacaa gaaagatttc ttccgtttct tgattaggca cagcagttgc aacatcagtc
    13321 ttatctgctt ttaaatctat gtcaggtaca ttttcagttg tcctaaattc actagatttg
    13381 gagccgctat caagctctct tgaaggttcc atcccttgat tgataacttg ttcttcttgg
    13441 gaagctaatg cttgttccgc ctgacgtctt tcttcttctc gagcagcttc tgccagaagt
    13501 ttcgcctcaa tccgagcctt ctcttgctct tctaagcgtt gcttagtctc tagcgccttt
    13561 gctctagctt cttgttcgtc tttcaaacgt tgcgcttcat aatctgccat caattgtgct
    13621 gttctgcgag cagcttcttt ggcttcttgc tctttttgat agagttcttc ttcacgcaac
    13681 tttctcaaac ggttagcttc ttctaactct gccagaattt gttctttaaa acttttctga
    13741 tcatggttag aatgctgatc gtcattaaat tcggtcaaag agatttcctc ctaataataa
    13801 tcacaggcaa ttatatctta aatagtctat aaaatcaaca aaactttgac aatgttatct
    13861 acttttaaca aattgatcgt gttggattat tccaaatcat attataccaa agcgaaccac
    13921 catgttggga gtcagattct ccctcatctt taaatccatt catttcatag aaggaaatta
    13981 aatcatcatg gcaagttaaa cttataccat ctcgttcttg agaaacaacc aggtctttca
    14041 tcgctgctaa taatgctgta ccaatcccct gacctttaaa atcaggatgt attgatagac
    14101 tagtaattcc tatgaaacca cctaccctta cagggaattc agaaacttta tggaataaat
    14161 cgtccgttaa gtaccgacct ttaataaccg gtccttcaat gtaacctgct aaacgaccat
    14221 taatctctgc aactaaaaaa gtatcagtca ttatcgttaa acgttcttgc attgcttttt
    14281 tactcgcagc ctctgcttct gaaaaattta tttgttcaat actaatgact tgctgtaaat
    14341 cctctaggca accatttcta ataatcatac tcatctcctt aatttcttta tgaaattctc
    14401 ttgataaagt cagatagagg actgagaaac ttctctcagc ccttaccatc aacttattgt
    14461 gtatttttag ttaggttagc taataattct tcaaaagagc gctcatacaa ttgaatgtct
    14521 ccagcaccca taaagacata gacagcatta tcatgattga gtaaaggcga gacattttcg
    14581 actgtcacta aatctgagtg tttgacaatc ttagcagcta aatcttctac cttcacctca
    14641 ccattatcta cttctctagc agaaccatat atttgagcga gataaacgct atccgcttga
    14701 ctcaaggcat gggcaaattc gtctaaaaga gctatcgtac gagtgaacgt atgcggttgg
    14761 aaaatagcta caatttcttt tgacgggtat ttttgtcgag cagcatctaa tgtcgcaata
    14821 atctcagtag gatggtgagc aaagtcatca ataatgacag tatcgtcaat aatcttctca
    14881 gtaaaacgac gctttacccc tgaaaatgtc ttcaaatgct cagctactaa tgccatatca
    14941 attcccatta tgtaaaggtt agcaataaca gcagttgcat ttaagatatt atgtttaccg
    15001 tatgctggta catgaaactg accaatttct tcttggttat agaaaacctt aaagtcagaa
    15061 ccattaacag ttcgagtgat gtcttttgct ataaaatcat ttgaatcttc aaaaccataa
    15121 taatatattg gtgcctcaga agtgatttca tgaagttttg gatcttctcc ataaatgaat
    15181 aaaccttttt gaacttgctt agcatagtca ttaaaggcat tgaatacgtc ctctaggcct
    15241 gtaaaataat caggatggtc aaaatcaata ttggtaataa ttgagtattc tggatggtac
    15301 ggcataaaat gacgttcgta ttcatcagct tcaaacacaa agtaattagc attagcagaa
    15361 ccacgtcctg taccatctcc aattaggaaa gaagtgtctg taatattttt taaaacatga
    15421 gctaataaac ctgtcgttga ggtttttcca tgtgccccag ctacacctag actagtgaac
    15481 tgacgcataa aatctccgag aaattcatga tatcgtttaa aatgatagcc cttttcaata
    15541 acataagcca actcttcatt gttatctgga cgaaaagcat ttcctgcaat aatctctaaa
    15601 tcctcactga tattattcgg tgagaaaggt aatatagtta cacctgcttg ctctaaacca
    15661 cgttgggtaa aataatattt gtcaacgtca cttccttgga cgttatgtcc catttgatga
    15721 agcatcagtg ctagggcact cattccggat cctttaatac caataaaatg ataagttttt
    15781 gacatggtct ctccttaatt aatgtcttta aaacgtgtca gatttaattc ttgagccttg
    15841 gctctttctt tatgaaactg attgtcttgt ttattgtaaa cttgactgcg tttcaaaaaa
    15901 tcaaaattat ttttctttgt ttttgggttc ggtgtattat caggttcttg aaatatgatt
    15961 ggaatttctg ctaatatata gctttcctgc tgtaaacgat cagaaagatg acttagttca
    16021 gtcacctttc gctgagtttg ttttttcggt actgttgcct tcaattgttg ctgaaaagct
    16081 tcctttgatg gcgccttaaa ttcctgtttc gcatagtaag cttgtcgttt ctcccgaaca
    16141 gacctctttg cctcttgtcg agatttgctc aactctgtcg ctttttttac aaaactctgc
    16201 ggtggtacag gatcattttc aattacctta ggatgtgata tttcttcatg ttcataattg
    16261 cttttgacca tgctttgata tgttttttct tgataaaagc ctcgaatgtt gttaattaaa
    16321 tcttcattat cataaagatt catctttaca agcggagaca ttactggttc atcatctgct
    16381 atcaacggaa atcgtctaga gcctctattc acattatcac ttcctttcta caacagacct
    16441 attataaact aatttttctg ttttttcaaa gttttcaact aagtaaagct tatcagattg
    16501 gtatttttca ttcatttata taaaaaagaa agcgagagac cactttcttt tttatattat
    16561 agtcacatca tcatatttat ttagaaactc caagtatctc tcgaatatca tctacgctca
    16621 tgctagcatg ggtttcattt ccatccagaa ctgttgtaac caaatgtttt ttagtttctt
    16681 gcatttctag aattttttct tcgattgtgc ctctagtaat caagcgataa acctcgacat
    16741 tctctttttg ccccaaccta tgtgcgcgac taatcgcctg catttcaaca gctggattcc
    16801 accacaagtc aatcagaaca acggtatcag ctcctgtaag gttgagacca acgccaccag
    16861 cttttaaaga aatcaagaaa gcatcttttg atcctgcatt aaatgctctg gtcatttcat
    16921 gacgttcatt tgctggtgta gaaccagtaa ttttataagt tgtaagcccc atagctacca
    16981 tctcacgttc tgcgatatct aacatacccc tgaactgaga aaaaatcaga gcacgatgtc
    17041 cattttcctt aatttgtgtt aaaagttgac gtaggctttc taacttaccg ctttcaccat
    17101 cataatccat aaaaagacgt ggagtgtcac aaatttgacg taatcgtgtg atgccagata
    17161 aaatttcaat tttacgacga tttaagtcag cgtcactaga atgttggata ctttcttgaa
    17221 tttggcgtaa ttgcgccaaa tagataactt tttgactgtc tgtcatttca tttggatagt
    17281 tcatttcaat caaatccggt aattctggga gaacttcttc ttttcgacgt ctcatcacaa
    17341 atggtttgat ataacgagca acttgtttag ggttcaactt caaaaactct ttcttaccag
    17401 gtaataaacc aggtaaaatg atctggaaaa tggaccaaat ttctaacagt ttattctcaa
    17461 taggtgttcc cgataatgca aaacagtttt tgacctcaaa agatctcaag ctatgggcta
    17521 ttttagtttg agcatttttc ataacctgtg cttcatccaa aattaaataa tcataatttc
    17581 cttcagaata agtctcaaaa tcctgcctaa atgatgaata acttgtaatg attacttgat
    17641 gacgttcagc aataatttca tctctacgaa tcttagaacc ataggcaact gctacatcta
    17701 cgtctggcgc aaatttgtgg aattcatctt gccaattata aattaaactt gatggtgata
    17761 aaataagcac acgagaatct ctagttagtt ttgtacttaa aaatgaaatc gtttgaagtg
    17821 tttttccaag ccccatatca tcagctaaaa tccctgcaaa gccataatga tctaacatac
    17881 ttaaccaacg gacacctgta agctgataat cgcgcaagga agctgaaaca gatagaccag
    17941 gtattttaaa cttttctggg tgtctcaaat cttcaatcaa ttggtaaaaa tgttgtgaaa
    18001 agctaacatt atcttgattt gcaaacagtt ctgataattg aaatgctgca atattatcta
    18061 attcaatatg cccattcttt gctcgtctag ctctcaaccc ttgcaaagtt gcacttactt
    18121 tcttagtttc ttcatcaaaa ataactaatt gccctgattt attcacaaaa taaggattat
    18181 tgtcaaacaa agctaccaga gcttgatcaa catcgttttc cagtaaatca gagaaatcaa
    18241 aagaaatatc tagcaggctt cccttagctt caatatcaat ttgcggtcgt tctatgagac
    18301 gataactttc caattttgcg gataagctca ctgttcccat ttttttaaat tgtggaagca
    18361 tataggtata aaaatcgtat aattcttgtg cactgtatgc tggatggctg gttgaaaagt
    18421 ggggtgcaaa gccatatttt tctaagagtt taaatacttt ttcttcatgt ttgaagtgac
    18481 tagcaaatgt taactgttct agttcttgac gattatgaac agttaaatca ttaccataat
    18541 caaataccat ttgcaataaa atttcatttt gactattaat atcgaactca aaatttacct
    18601 taaaatcatg aatagagaag ctccgtggag catccactag tccaatctcc ttaaaatcca
    18661 gtaaatgagc tgctaactta tcttgatcat ctaaatcaaa atggatatgt tttgctaaat
    18721 ctccctcaat gggaagacta cgaatagccg ttaccatctt ttcttgcttc aaattaagat
    18781 ggtagaaagt atcgcgataa aacaaataag aattcgcaaa tagtggtttt aagtctttct
    18841 ctttgatttc taattcaatt gattgcctat ggacaataac cttaaattga taaagatgat
    18901 cctcaccttc taattcctta aagtagaggt gattaaattg atgaaaatca ctttcaaatg
    18961 aaaaattttc taaagcattc atcaaagtaa caccctcttc aaaaaaacca cgagtgagac
    19021 taaggtgacg accttgattt ggtaaaataa actctagatc aattttgctt gaatgacttg
    19081 gaatgagcct ccataaaaat tctatcaatt cttgactggt ctcatcaaac tgtatcaaag
    19141 acaaagtttc aaagtattgt tttccaatct gataataagc ttcttttcga acagtattta
    19201 aaaaggcttt gatgtcacga ataacataac ttctatcatc aggcagtcgt ctaattttta
    19261 aagtccacca gatatcatta gcataaggat tttgttctcc ttgagccgat aattgatatt
    19321 ttatagtatc atcttcatta ataattagac tatctaaaaa aacactacca aaagaggttt
    19381 tcttcttagt ttcttgttga ctttcttgtt gaacttgaac tttagataag attgctttac
    19441 cttctggatc gtttttcaag aaatgctcca gagcagctaa atgttgacaa tagccttttc
    19501 ttgcaaaaaa atcacaagaa cattttgttt cttctgttac taatgaatat tcaatctgac
    19561 aatccccaac tttagctttt aatagattac cttcttgaga aatcaaggac accaaacctt
    19621 gttcgtagag ttctatacct tgatttcgaa tacgtcctgg aatcattcta ctcattatgc
    19681 taccaccttc atcttatccc ttaattataa caaaattcaa gtccaatttc ttgtaaaaat
    19741 actaaattga ttgttttttg atacagtaac aaataaaaaa ggctaaaaca actgtcctaa
    19801 ccttctattt caatttcaac aataagatgt tatttacgtt tacgtgctat tagattaatt
    19861 ggtgttcctt caaaaacaaa tgcttcacgg atttgatttt ctaagaaacg aagatatgaa
    19921 aagtgcatca attcttcttc gttaacaaaa acaacgaaag ttggtggttt aacagcaact
    19981 tgagttgcgt agaaaatctt aagtcgtttt cctttatcag ttggtgttgg attaatagca
    20041 atagcatcca taatcacatc atttaataca gcagacggaa tacgtttgtt ttgactttcg
    20101 ctaatacgtt taatcatatc tggtaacttg tgcaaacgtt gctttgtctc tgcagaaaca
    20161 aagataattg gcgcataaga caagaattgg aagttatcac gtatatcagc ttcccactga
    20221 ctgacagtat gattgtcttt ttcgatagta tcccatttat taactacaat aataatacct
    20281 ttaccagttt catgtgcaaa ccctgcaata cgcttgtcat actcacgaat accttcctct
    20341 gcattaatca ccataagaac aacatctgag cggtcgatgg cgcgcataga acgcataaca
    20401 gaatatttct ctgtgttttc atatacctta ccagatttac gcataccagc agtatcaatc
    20461 atagtatact cttgcccctg gctatcaacg aaattagtat caatagcatc gcgcgtagta
    20521 ccagcaactg ggctagcaat aacacggtct tccccaagga tggcattaat taaactagat
    20581 ttcccaacat ttggacgtcc aatcaaacta aaacgaataa tatctggatt ttcattttcc
    20641 tcttctaccg gaagattttc cacaatagca tccaatatat caccagtacc aataccatga
    20701 actgatgaca agggataagg atcccctaaa ccaagagagt agaaatcgta aatatcattg
    20761 cgcatctcag gattatctac cttattaaca gctaaaataa ccggtttatt ggtcttataa
    20821 agaatacgtg agacatactc atctgcatca gtcacacctt ctttacccga cacaacaaac
    20881 acaataacat cagcctctgt catagcaatg tctgcttggt gcttaatttg ctccataaac
    20941 ggtgcatcaa catcatcaat cccaccagta tcaattaatg aaaacttacg atttagccac
    21001 tcacccgttg tatagatacg atcacgagtg actccttcaa cgtcctcaac aatggaaata
    21061 cgttctcctg caattctgtt aaataatgtt gattttccaa cattcggacg accaacaata
    21121 gcaactgtag gtaataccat aattctcctc tcactattgg gaatactccc aattccattc
    21181 tattttatta acggcgattc ggtccttcta agtggaattc tatcgctaaa taccgtacgc
    21241 gctccataac tcgttttgct tgccaagttt cgtcgcttcc ttttatatta gcccattttc
    21301 tttccaaatc gttaaaacta taatttgacg taaagaaagt gggcaactct tctagcatac
    21361 gatgttgtaa tataacttgt aaaatctcgt cacgaaccca ggacgttgcc tgttcagccc
    21421 caatatcatc taaaatcaaa atcggaacac ttttcacagc atcaatttca tcttttacag
    21481 ttccgcttga aatagcattt ttgacatcaa tagcaaaact agggaaatga agaagagtcg
    21541 ttgacacacc ctttctctct gataattccc tcgccattgc tgccattaaa taggatttcc
    21601 caacccccat atctccatat aaataaagcc ccttgtgatt atatgaagga taagtctcta
    21661 caaaatcaag cagttgtgac atagctttca ttcgactctc gttattaata tcaaagtcag
    21721 tcattctgat attccgatag gacttgggta gattaactaa attaatacga tcactgatag
    21781 cttgtttttt ttgtgcttca attaactctc gtgtttctaa ataagaaaca tctgcataac
    21841 cttcattcat aaccaaaata ggctcatagc ctttagcaat atattgacta tccttatttt
    21901 taaatttctg acgctcaatc aaaaattgat taaatttaga catgctgata tttatttcac
    21961 gctgagaaag ttggtgggtt ttaatgaact ccgctacctg agcatctgct aaaatcattt
    22021 gaataagctc atcatttgta ttacgaggca ctctcccttg attttctaat gcttgtccca
    22081 ctgatttcat gattcaccat cctttcctaa tcgttctaaa cgcttgaggg catctgtttt
    22141 aaactgttct aattcaattt ctttttctgg gctaacctca tctttataat caggattact
    22201 ccattcagga acattcgatt gtttcttctt cgtttctgtt ttagtcctta ctttttgatc
    22261 tgaaaaatca cgaattttaa gcacagcatc ttcggccgtc ataacattct gaaaggcaaa
    22321 atcattagca actttcatga tataggcctt atttaagtta gcagattttg tcttattcaa
    22381 ggtatataaa accataacat taatgacttc gtctaggaaa ttcatttttg ccaaatcctc
    22441 aagtaaagtc ttctcaccac ttgttgtcac tgccttacgg cttctcttga ttttttcaag
    22501 gaacactaga gcactatcat ttttagattc ccttagtatg accttttcat tatttgtaaa
    22561 actctgatta tctttaatat gctgcccctc attttgttga agtttcattc gctgaggcgc
    22621 aatcattcca ttgatagctg tttgttttgc taattgatag gtatcatacc aatttaaatg
    22681 ataaagttct gatacactat aaatacctaa gacatcatct ttttcattgt taaaacgtaa
    22741 gccatctcgc atcatcaaac gtttgaaact ttccaagtca aaaacatttt tactccggtt
    22801 tacctcttgt tttggttgtc ctaaatccga gaaaacatct gtaaattttt tagagatatc
    22861 tctagcattt ttaggtattt tcatatctaa ttctgccact gctacctctc ctatacgttt
    22921 ttctaacaaa cgacgataga tgggattaga taaaaataac tcatttgaca tagcttgatt
    22981 caacttaaca aggtaagtac ctgtggcttg gtaaacagtc aacaaatcca aagctgttaa
    23041 cattaccaat gcatcttgaa atcttggcat cccgtattgc agatgattga gaacctctga
    23101 aaatttatga cgtcgtattc cgtcatcaaa aaaatgaaca aaataatcat agagtgccat
    23161 ggcatcactt cccataattg ggaaatacaa ttgtattaaa ctattactat cataaacaat
    23221 cttattttgc ttaatatatg taaactcatc tattggactc atcacacgct accactcttt
    23281 ttactccgta cgcgctttgt aatttgttga agtagttctt caatttcatc tacatcttta
    23341 aaagatttat aaacactagc aaagcgcaca taagtaatct catctaattc ggcgagttca
    23401 tccatcacta aatttccgat agtgatacta gaaacctcat cttcatactc actcctaatt
    23461 ttacgttcga tacgtaagat acaattttca atatcttcgc tcgaaacagg gcgtttttga
    23521 gcactctgaa taataccatt caaaatttta tctcgagaaa attgctcgcg agtaccatct
    23581 ttctttatga ctaaaagagg caattcttcc aaacgttcaa atgttgtaaa gcggttgcca
    23641 catttttcgc actctctccg gcgtctgata gtcgtacctt cttcagcttg tcggctatca
    23701 accacactag atttattata gccacatttt ggacaacgca tgtcaacacc cctattctat
    23761 catatcattt aatcatctta ttttaacaca aattcagtaa taacaaaaga agcgcctctc
    23821 ttatcacctc atcatatcaa aacacaaaat aatccttatg ccttttatca ttaagcataa
    23881 ggatttttct tatatttctt tagtttcttc aaattttttt ataggaatgc gaacaatgaa
    23941 ttcagtccct tgatttagtt cacttttaat atccaccttt aaatggtaag catccataat
    24001 ttgtttaaac actgaaagcc cgatacctaa accagcttgt gtactttctc tgttacgtga
    24061 tttatcagtt cgataaaaac gatcaaagat aaactcaata tcttcatctg agataccttc
    24121 tcctttatct tttacaacta ctgtcgcaaa atcattgtct actgataaaa caacactgac
    24181 ttctttataa ctaggagagt acttgatagc gttatcaatt aagatcatga gagcttgctc
    24241 aaaatgtatc ttatatatct ttccccaaac gatatctgaa atattatttt caaaaataaa
    24301 ttgaaaatct tctctcaaaa tacggaaatt ttcaataaca actgatatag agcttgataa
    24361 atctgtcact tcatcttgat gaagttccaa gcttccttgg acgcgaatca tatttaacat
    24421 gtcattaatc attaaggaca tacgatctgc ctcatggtat gccgctgcta aactttcttc
    24481 aagaatttct ggatcatctt tcccccaacg ttgtaataaa ccaatatgac cctttacaac
    24541 agcgacaggc gttcttaatt catgactaac atcactaatg aattgtgatt gtcgacgagt
    24601 atagtcttcc aactgatcca acatcccatc aaaaatccta gacaattctc caatttcgtc
    24661 ttctgaacgg accttacttc taatttctaa attactagga ttttcagata tctgatgcat
    24721 taagtcgtgt aaatttttaa ccggacgaac aatacgctta gtagcactat taataacgac
    24781 caaggctaaa acagtcccta aaacttccat cataatcaag aaaacaatga tgttttgcct
    24841 catactataa taacgaccta aattatagaa aatttgaacg tatccaagca actgtcctgt
    24901 tttttgagag tagattgctt ttgtaccaac aaaaccttcc atatctgcaa ctttagagat
    24961 gataactttt cccatggatt taccaatagt tggcataccc gttctcagtg tagaaaaaac
    25021 tggtttcctg ttgacatcgt atagggtcat ttcttgatta acataaagaa tactatcgat
    25081 tgactcgccc gttctaacaa ttccattttg acgcttatga ggatagatgt catccgcaaa
    25141 aacctggttg tacaggatct ctaaaatatc atcggatgtc aaactagagt tcgcttgcga
    25201 caaacgaact ctagtttttt ctaatgacct accgacatta attttttccc tgtgaaggac
    25261 gtatcttgtt gccgaagtat aaactaaaac agaaaatact gtaaataggc taaaaaataa
    25321 gataaaaact aattgcgata atttttttga cagaggttgt tttaccccta taaactgatc
    25381 ttttttattt ttcacgaatc acatagccca ttccgcgaac agtttgaata tatgattcac
    25441 ggcctgggat atcaatttta cctcttaggt aacggatata aacatcaaca acgtttgtct
    25501 ctgctgccac atcgtatttc caaacatgtt ctagcaactc ttctcgtgtc ataacacgat
    25561 tcatatttgt catcaagaca ttcaacaaat caaattcacg ttttgttagt gaaatttcct
    25621 catcaccacg catcgctgaa cgattatgag tatttaatga caaatcacgg aaactaccac
    25681 tatcaccttt ttcttttgtc ttcgtttcaa tttcttggcg tcggaaaatc gctctaacac
    25741 gtgctaataa ttcttcgatt gcaaacggct taacaatata atcatctgct ccacgatcaa
    25801 gaccagctac aatatccata acagaatcac gtgctgtcat catcatgata taggttgttt
    25861 tttcagcctg caggcgacgt gtgatttcga aaccatccat ctctggaagc attaaatcca
    25921 gtagaatcaa atcaaaatct ttttctaatg ctgtgtccaa tccttcacga ccgtttgttt
    25981 caacaacaac atcatatcct tcatgtagta gttctaacga gacgaagcga gctaaatttt
    26041 tctcatcttc gattattaag atctttttac ccatctacac caaccttctt attctttact
    26101 taacactatc aattattttt tagaatatct agataaaaaa caagatagcc cacattttac
    26161 taacgtttct ttaaataaat aaatcgccat cccaaacaat tgttcgatag cgagacttaa
    26221 tttttattat tgagtagtgt cctctgtcac ttttcaattc tactaaaaaa aaattaaaat
    26281 atcaactgat gttattaata atttttatct aatcactatc aaacatacct tctaaggctg
    26341 cgaaaggatt agatttttct ttttctttag cttgtttttc ttcatactgc ttttcagtca
    26401 ttaacgacca atttttacct gataaatccg cttctacccc aacctcatca gctgccagaa
    26461 cacgaagagg tatatttaac aagatattat cgataacgct ttcttctaaa ttaatatcat
    26521 cttcctctaa tattaagacc aactcctgat caacgagctc ttttttagtg ctaacatttt
    26581 cagcttcaat aaaaacttca ttaatagttt gcttttcaga taaaacaact ggtttcatag
    26641 aacgactaga cggtaatgta atatcgtaag ccatattata gtcaagtaga tagagaccat
    26701 cttcatacac cacatgacct gaaacttgaa tatcagaaat atccattatc tctgaatgac
    26761 gctccataag cgactctttg atatctattt ttttatcaaa ataaagcccc tctggtgatt
    26821 ttttgatttc tgtaagtaac acgtgccatc tccctttata gtatatgtca tattatatca
    26881 agttttacag gctttgtctt aaaaaaacaa aacgcaaggc atagctaccc tacgttttgt
    26941 taacgactac atatgacgca aacgctcaat acgatctgca attggtggat gcgtataaaa
    27001 taaagacttt aacccttctt ttttcgttgg gtcattgata tagagagccg cactagcatc
    27061 atctactgga tgccccatag gttcagaacg atcaagtttt tctaaagcag agatcattcc
    27121 ttgtggattt cttgttaact caacagaact agcgtctgct aagtattcgc gctgtcttga
    27181 aatagctaat tgaactaaac tagcggctaa tggtgcaagg attagtgata aaatagagaa
    27241 aatcaaaacc aagatgtttc caccatcttc tctgtcatca tcacgcctac gacctccgcc
    27301 gtaaaataac atacggctac caatgcttga tattagagtt acagcactcg ctaaagctac
    27361 tgcaatggtt gaaatacgga tatcataatt acgaatatga ctaacctcat gtccaataac
    27421 accctctagc tcttcgcggt tcataacagc taataggcct gtcgttgctg caacagcagc
    27481 attttctggc ttagagcctg tggcaaaggc atttagggag tcatcttcaa caatgaaaac
    27541 gcgtggcata ggtatctgag ctatcattgc catatcttca acaatatgaa aataatttgg
    27601 cgcctcatct tctgtaactt cacgcgcatt gttcattgac ataacaacat tagttgattg
    27661 aaaaatcata cttactgcat agataacccc aataattaga gctagaacaa gcccaaattg
    27721 gtagctgcct aataccaaat aacctacagc tgctccaata gctgctaaaa gacagaagaa
    27781 gacaattaac aagacaactg tttttctctt attacttgca atttgttgat acagcaaaag
    27841 cctctctccc ttctcaatta aaagtcaaag gaaacttttg gcacttcttt ttcttcttca
    27901 ggagtttcta agaattggct tggtttgaac ccaaatagtt ttccaacaat attacttggg
    27961 aaagtttcta atttcacatt ataattggat gttgttgtgt tatataactg acgtgaataa
    28021 gaaattttat tttccgtatt agttagttct tcttgaagtt taacaaagct attgttggct
    28081 tttaaatctg ggtaattttc agcaaccgct aaaatacttg aaagctgtct tgttagctca
    28141 ttacttgctg tcattgcttc agcaggtgtg ttagctttag caacttgagc acgcagttct
    28201 gcaatcttct caagtgtttt cccttcgtac gcagcatagc ctttaacagt ttcgattagg
    28261 tttggaatta aatcgttacg acgtttcaat tgaacgtcta tttgactcca tgattcttta
    28321 gtatgcatgc gacttctaac taaactgtta taagcaacaa tcaaccaaat aacaaacaaa
    28381 gcaataattg ctattaaaat catagttccc atagtagtag ctcctttaca ttttttccat
    28441 tatagcaatt ttctttagat taggaaagtc ctgttattcc cattattagt atttgtgata
    28501 aaatagagtc attatacgat atagaaattg aggtttacta tgacacccca agcattttat
    28561 caagttctga tagagcatgg tattacactt actgacaaac agaaaaaaca atttgaaact
    28621 tattttagac ttcttgttga atggaatgag aaaattaacc taacagctat tacagataag
    28681 gaagaggttt acttaaagca cttctatgat tctattgcgc cgattttaca aggttatatt
    28741 gacaatagtc ctcttagcat tttagatatt ggtgctggtg caggttttcc tagtattcct
    28801 atgaaaatcc tttatcctga aattgatatt actattattg attcactgaa taaacgcatt
    28861 aattttctaa acatattagc taatgaactt gaactttcag gagttcactt cttccatggg
    28921 cgcgctgagg actttggtca agataaagta tttcgagcta aatttgatat cgtaacagct
    28981 cgtgcagtag ctagaatgca ggttctagca gaattgacaa taccttttct taaagttaat
    29041 ggtcgcttga ttgcactgaa agctgcagct gctgaagagg aattaatttc tgcagaaaaa
    29101 gctttgaaaa cactattttc acaagtaact gtaaataaaa attataagtt acctaatggt
    29161 gatgaccgaa atatcacaat cgttagcaag aaaaaagaaa cgcctaataa ataccctcgt
    29221 aaagctggta ctccaaacaa gaagcctttg taaagttggg agctagtatg aaacatttct
    29281 ttgattacaa gacaatgtct gtcgcacgta aactcagcat tagttttatc gctgttatcc
    29341 tattgggaag tattttactt tcacttccaa tttttcaata tgctaacgcc cctaaaacac
    29401 attacattga tcaccttttc acgactgttt caatggtatg tgtaactggt ttatctgttt
    29461 ttcctatcag taaggtttat aacggttggg ggcagattgt agctatttta ctcatgcaaa
    29521 cagggggact tggtttagta acactaatgt cgttgtctta ctatacccta agacgtaaaa
    29581 tgtcgttaaa tgatcagacc cttttgcagt cagcgataac ttataacagt tcgacagatt
    29641 taaaaaaata tctctatatg atttttaaag taactttaac tttagaagtt ttagcagcca
    29701 gcattttagc catagatttt attcctcgat ttggtttgag tcatggtatc tttaatagta
    29761 tattcttagc tgtctcagcc ttttgtaatg ccggttttga taatctaggg gcaacgagct
    29821 tagctcaatt taaactaaat cctttggtta acattatcgt ttgtttcttg attatttcag
    29881 gaggacttgg atttgccgta tggaaagatt taattgaagc tactatacaa acatctcaca
    29941 agggaccaaa attaatcaag acatttccaa aacgcctcag caaccattct aaactggttt
    30001 taaaaacaac aaccattatt ttactaacag gaactttgct ttcttggttg ttagagtttg
    30061 gaaattttag aacaattgct aatttatctc tacctaaaca actaatggtc agctttttcc
    30121 aaacagttac gatgcgtacc gctggatttt caactattga ttatacccaa actgatttcg
    30181 caaccaacct tgtttatatt atccaaatgt taattggagg agctcctggt ggaacggcag
    30241 gaggatttaa agtcactgtt atcgccattt tactcttact atttaaagcc gaactgagtg
    30301 gccaaagcca agtgactttt cattatcgaa caattccttc cagtattatt aaacaaacac
    30361 tatcgatttt aactttcttt tttatcatac ttattagcgg ctatctcctt cttctggaac
    30421 tcaatcccca tatagatcct ttttctcttt tctttgaggc atctagtgcc ttagcaaccg
    30481 tgggtgtaac aatgaacact accaatcaat taactctggg cggtcgaatt gtaattatgt
    30541 tcctcatgtt tattggtcga gtcggtccaa ttaccgtact gctatcaatt cttcagaaaa
    30601 aagagaagga aattcactat gctgaaacag aaattattct aggttagaaa aggtctttta
    30661 tgaaaacaaa aattattggt gtacttggtc taggaatatt tggacaaaca ctcgcacaag
    30721 aactaagtaa ctttgaacaa gatgttattg ctattgacag caatcctgaa aatgtacaag
    30781 ctgtcgccga agttgttaca aaagcagcta tcggagacat tactgattta gctttcctaa
    30841 aacacatcgg gatcagtgac tgtgatactg ttattattgc tacaggaaac agtttagaga
    30901 gctcagtatt ggccgtaatg cactgtaaaa agttaggcgt cccacaagtt attgctaaag
    30961 ctcgaaacct tgtatacgaa gaagtacttt atgaaattgg tgctgatttg gttatctctc
    31021 cggagcgaga atctgggcaa aatgttgctg caaacctcat gagaaataaa attacagatg
    31081 tcttccagat tgaatctgat atttctgtca ttgaatttaa aattcccaaa tcatgggtag
    31141 ggaaaactgt cgaacaatta aatatccgtc acaagtttga tcttaacctc ataggtatca
    31201 gaaaagcaaa aaacaaacct gtcgataccg aagttccaat caatactcca cttgaagaag
    31261 gtattattct agtggctatc gctaactcag atgccttcca acgctatgac tatttaggat
    31321 actttaacta aagaaaataa aaaactgact attaaagtca gttttttatt gccatgggtt
    31381 atagaaacgt ccatgattaa ggtgaattaa ataaaggctt acaaacagac tagctagagc
    31441 aagtatgatt aagacaatgt cacttttttc caaagattgc ttagagtacc aagtacgtct
    31501 tttattttta ccaaatcgtc gaagttccat agcagttgct actgtatcaa tacgttctag
    31561 agagctaaaa attaatggtg tgacaatctg caaattcccc ttaatgcgtg ctactaagtt
    31621 cgattttttt gataattcta ttcctcttgc ctcttgtgct cgacgtattg tataaaattc
    31681 ttcttgaaca tcaggtatat atctaagggt caaactaaca gcataagcta ttttatatga
    31741 taaacctaat tgatttaaac ttgatgcgaa ctgactagga ttagttgtca tcaaaaacaa
    31801 aagagctaaa ggcactgtac aaaagtattt taaaatcaga ttaaagaggt aaaacaactc
    31861 ttggctagtc acatcatatg ggccaattcc ttttaacaat aaagaagaac taccataaat
    31921 cttatcacca taatggggag caaaaagata gaccattata atatttaaaa cagcgaatac
    31981 aacaactaaa gtcataacaa aggacacctg ttgccatttg atttttgcta atctaaataa
    32041 gactagcgat cccaatgaaa caacaattaa aaatcttgta tcataagtgg tcatgcaagc
    32101 aacagaaact aaaataaaaa atagtaactt actggcacct gatagcctgt atagaaagcc
    32161 ataaccctct tgataaccaa ttaattgttt agccattctt acctccttga ttatcaatgt
    32221 accattgtgt tagtttctgt ggagatatcc ctaatctgtc tgctaaagca aataaactcg
    32281 ttttctttaa atgagtcttt ttcaaaagct ctgtatttga aaggatgtta ataggatgat
    32341 catctgcaac aatcagacca ttgtcgatta caattgcacg atctgtatac tctaacataa
    32401 gctgcatatc atgtgtaatc ataacaatgg tatgtccatc acaacttagt ttatcaagga
    32461 aagacatcat ttcagtataa tgcttcatat cttgaccagc tgttggttca tccaaaataa
    32521 taacttctgg atttaaaatc agtatcgaag caatcgtaac acgttttttc tgaccgaatg
    32581 atagagccga tataggccaa ttcctaaatt ggtagagccc acaaaccttt aatatatcgt
    32641 agacacgact ttctatatca ttatcactaa aacctcttaa acggagaccc agagcaactt
    32701 catcaaaaac catcgcttga cttatcatct gattgggatt ttgcaaaaca tagccaattc
    32761 gttctgctct ttcttttata gagtcttgac tgacatcttc attattataa attagagtag
    32821 cattattagg gataaattga cataacgctt tagctaaagt agatttacct gcaccatttt
    32881 tcccaacgat agctaaccgc tgacctttat ataaatcaaa cgaaacatca tctaaagtat
    32941 tattctctaa atcatatgac accgacaaat gctgtactga taataattta tctgtatgtt
    33001 tatctaaatc taatgctcgc attttaggta tcaatagttc actgaaatca aagtcatcaa
    33061 tagacgtcat tgtattttgt ttttcaaagt catatcctaa atttttgaga gcagaaatat
    33121 acagtggttc tcgaatacca tattcagata aaaaattagt ttttaaaagt tgatccggct
    33181 ccccattata aataatttgt ccatcactaa aaagaactac acgatctact cgctcataaa
    33241 gaacatcttc caatcgatgt tcaataataa tagtggtcgc atcttgctct tgatgaatac
    33301 ggtcaaccaa tgccatgata tcttgtcccg attgtggatc tagattagcc aaaggctcat
    33361 caaataatag aattgggcta tcgtctacta aaactccagc taaactaacc cgttgttttt
    33421 gtcctcccga caagtcttgt ggacgtttag acaaaagagg agctatttct aaacgttttg
    33481 cccacatttc aacgatagag gccatttctt cctgtgctac aacatcattt tctaatgcaa
    33541 aagcaatatc ttctgctact gtcaatccta taaattggcc atctggatct tgtaaaaccg
    33601 tagaaactaa atgagacttg tcatatattg acaagtcaaa agcattttta tggttaatcg
    33661 taaaaatacc actatattgg cctttatgag tatttgggat aataccattt aaacaatgac
    33721 caagcgttga cttccccgac cctgaaggtc ctaatatcaa gaccttttca cctttaggaa
    33781 tgcttaagtt aatccctttt aaagttggtt cagattgaac gtcatattga aaggtaaaat
    33841 ccttccattc aataaaatct ttcatagatt actctcttat cctaatcttt ggataaacta
    33901 cctttttgtg gtcgtgattt agcgtatgct agcaacaaaa gcgtaccacc tactccaata
    33961 gtaatggaat tcacaaggct tgataagaaa ccttgtgcaa aaactttgct tgcaggttcg
    34021 ctataaaaga aaatatcacc atacggtgca acgacagacc aacctataag gttagcaata
    34081 acttgaacta cattaaatag aagcaaatcc ttcttaatca tgccagataa ttgaatagct
    34141 aattttttag caaaaaagcc tatcatcaaa cctaaaagac cactaactaa aacccaagtc
    34201 caccacggag aaccatattg aatcgaatct ttcaatgcgt gtccgataaa accagtaaaa
    34261 aaaccaactc ctggtccata aataactgca aataatgcta aaactgcata ctgcaattga
    34321 atattagtat ttggaattgg tgttggtata ttaactagca tacctataat gataaaaagt
    34381 gcagctccaa ttccagtcgc tacaaccttt tttattgtgt ttgtattcat aaaaacctct
    34441 ctgaaaaaca agcttaaaca gtcacttaca atctgtttta ttttatcata tttcaagctg
    34501 atttgaaacc ttattgacaa gaagaactga aaaacgattg aaaataatat taaataccaa
    34561 aaaagatctc agatagaata cctaagacct tcattagttt atttttttcc aaaaactgat
    34621 ttttcagcta cagccgtgaa cagaacgtct gtcgatgaat ttaaagctgt ctcacacgaa
    34681 tcctgtatca cccctacaat gaaaccaact cctacgactt gcattgcaac gtcatttgaa
    34741 ataccaaata agctacatgc caccggaatc aataaaagtg aaccaccagt tacaccagaa
    34801 gctccacatg ctgatacagc agcaaccaca cttaataaga atgctgttgg aaaatcaaca
    34861 gtaattccta aagtatttac tgctgctaaa gtcaggatat taattgtaat agctgcacca
    34921 gccatattga tagctgcacc aagcgggata gatactgagt aagtgtcttt atctaatcct
    34981 aaatcctcac atagtctcat attaacagga atattcgccg cagaactacg tgtaaagaaa
    35041 gctgtaatac ctgaatcctt taagcatcgt aataccaaag ggtaaggatt tttacgcatc
    35101 ataacaaaag caatgattgg gttaacgaca agagctacaa ataacatcgt acccaccaaa
    35161 actaatatta aaagaccata atcacctagt ataccaatac cgttttctga aatgctagtg
    35221 aaaaccaacc ccataatacc aattggggcc aaattgataa tccatttgac aacttgtgaa
    35281 gtagcatcag caaaagtttg cattaaatct tttgtacgct gattactgct tcgcatcgcc
    35341 aaaccaaaaa taaccgccca aaatagaata ctcatataat tggcctcggt aatggcatga
    35401 atgggattat caacaatttt caacaaaaga gattgaaaga cttctgaaat ccccttggga
    35461 ggtaataagt ctgtctttgc tggcgttttc aatatcagtg tcaaagggaa aatatagttc
    35521 acaacaacag cgattagggc cgctgcaaaa gttgcagtga tatacaaacc aattatcgtt
    35581 gacatattgg tcttaccgcc ctcacggtgt tgcgacaagg cactaataac taaagtgaaa
    35641 actaaaagag gggcaatagc tttcaaccct cctacaaaaa gttgaccaag taaaccaatc
    35701 gcagaggctt tagggactaa aatacccaag aataagccaa taacgactcc aattgttatt
    35761 ttttttatca agcttgtttt tctccaagct tttaaaaatc gtttcatact aaactcctaa
    35821 aaaattctta aagtctatga tatgataaaa aaaaagattc gtcaaatacc ttttttattt
    35881 tttagaggtt gacgaattct aaatttttct gatataatta ctttttgaaa tattcaatca
    35941 atttcgaaaa taaaatattt ttttgtacag aaaggtacat tgatgaaact aaaaaacact
    36001 tatatcatta tcggcttgat gctttttgcg ctgttctttg gagccggtaa tcttatttat
    36061 ccagcctact taggtatcta ctctggttct aattgggcgt gggctatcat tgggttctgc
    36121 ttaacaggcg taactttacc cctacttggt gttgcagctg tgacgtactc aggagcaacc
    36181 gatgttgaaa gcttcaccaa acctgtctct aaaacatatg ctattttctt tgcagtagca
    36241 ctatacctat ctattggacc tttctttgcc attcctcgaa caggggcaac ctcattctca
    36301 gttggtattc aacctatctt aggcgacagc tccttagtca aaattatcta tggtttaatt
    36361 ttctttggta tttcttattt cttagctatt agacctagtc agattgtaga tcgtattggt
    36421 aaatatctaa ctcccttttt actaactgtt attagtattt taattattgc ctcactcatc
    36481 catccagctg ctatcgttgg aacaccacat aacgcttcaa cgaatgtcag cgatgctttc
    36541 aaggatttac cattcctagc aggactgatt caggggtatg gtacaatgga tgcccttgca
    36601 tcaattgctt ttgctattat cgttattcaa gcaagtaaac agtacggagc aattaccaaa
    36661 aaagaaatta cttctatgac acttaaatct ggtgctatcg ctacatttct tttagctttt
    36721 atttatattt ttgtaggacg tattggtgca acttcacaaa gcttgtttaa atttgcaaat
    36781 ggttccttct tacttcacaa tactccaata gacggtgggc atgttcttag tcaatcagct
    36841 aacttctatt taggaattgt gggacaagct attttaggta cggctatttt tcttgcttgc
    36901 ttaacaacag ctactggatt aatcactgct tgtgcggaat acttccacaa attacttcca
    36961 aagattagtc acattacttg ggctacaatc tttactttaa ttgctattac gttctatttt
    37021 ggtggtttat cagagattat ccgttggtca ctaccagttc tctaccttct ctatccatta
    37081 actatagtct taattttctt agtctttttt gatcaaaaat ttgaaagtag ccgcattgtc
    37141 taccaaactt ctattgccgc cactgctgtc gctgctttat acgatgcctt atcaaaactc
    37201 ggcgaaatga ctggtttatt taccattcca tccgctttga ccacattctt tactaaagtt
    37261 gttcctttag gtgaatattc tatgggatgg attagttttg caatttgtgg tgtcttagtt
    37321 ggtcttattc taaaaaaagt aaaagcataa taatttaaag cagtacgaaa attactgctt
    37381 ttattttaaa ataattattg taaccgcttt cttttggtgg tataatagcc ttgaaaataa
    37441 ataatttttg gaggaactct aatatgagag ctgcaactta tctttcagct aatgaattat
    37501 cacttactga taaggctaaa cctcaagtaa taaaacccac agatgcggtt gtaagacttg
    37561 tcaaaacaac catttgtggt actgatctac atatccttgg tggcgatgtt cctgcttgta
    37621 aaccaggaac aactttaggt catgaaggca ttggtatcgt cgaagaagtt ggttctgctg
    37681 tcaatcattt taaagtcgga gataaagtaa ttatttcttg tgtcactgcc tgtaatactt
    37741 gctattactg taagcaccag ttgccttcac attgtgaaga tggcgggtgg attttaggac
    37801 atcttattga aggtactcaa gcagaatatg ttcatattcc acatgcagat ggtagccttt
    37861 atcatgcacc tgaaggtgtt tgtgacgatg cattggttat gttatctgat attttaccaa
    37921 cttcatacga aatcggcgtc ttaccttctc acattaaacc aggtgatact gtttgtattg
    37981 taggcgctgg accaattgga ctatctgcct tactaactgc acaattctat tctcctgcca
    38041 aaattattat ggttgattta tctcaaaaac gacttgaagc ctctaaaaaa tttggtgcaa
    38101 cacacaccat tctatcaacg tctactcaag aagttaaaga agaaattgat aaaattacca
    38161 aaggccgtgg cgttgatgtc gtattagaat gtgttggtta tcctgcaaca tttgatattt
    38221 gtcaaaatgt agtttctatt ggaggacata tcgcaaatgt tggtgttcat ggtaaacctg
    38281 ttgaatttaa cctccaagat ttatggataa aaaatataac ccttaatact ggattagtta
    38341 atgctaatac aactgaaatg ctccttgaag ttcttgagac aggcaaaatc gatgcaacac
    38401 aactagtaac acatcacttc aagctatctg aaatcgaaga agcttataag gtatttaaag
    38461 ctgctgaaga aaacaatact ctcaaagtta tcatcgaaaa cgatattact ccagcctgat
    38521 aatttttaaa caaatgaaaa tgtgagaaga atagccttct cacattttta tttatgactc
    38581 aagcgacgtg ttaaaaaatc acctaaaaat tggataaaga aaattaataa taaaatcaga
    38641 atagtcgcta ctaaagtaat atcatcacga gaatagttat atcctttagt aatagcaaca
    38701 gaacccaatc ctcctgcacc aatagcgcca gccatagctg tttcacctac taaagaaatc
    38761 aaagtaaccg ttgatactcg aattaaatct ggtagacctt cacgaagata aactacaata
    38821 atatcccaaa gtgttccacc tgaggcttgt gcagcctcaa taactccacc atcaagttca
    38881 gctaaaacaa cttgaacttg acgagcaaaa aatgggaaaa ctgccaaaga aagaggtacc
    38941 aaagctgctg gtgaaccaag tgttgttcct acaattacgc gagttactgg cgcaatcaaa
    39001 gcaagaagaa taatgaaggg cagagctcta aaaacagaaa caactttatc taaaactcca
    39061 aatactaatt tattagcaat aactcctcta ggactagtta aaacaaggaa taaacctcct
    39121 aacaatccca ttaaacctcc aataaggaac gaccaaaaag tcatataaag ggtttgtaca
    39181 atagctgtct gccagccgta agcaccttcc caacccattt gatatacatt tggtaaatgt
    39241 gtttgaatcc actctatcat gctttaccac cttctttcaa taacgttaat tgaacttgcg
    39301 aatctgtaat tgcttggcag gcttgtctta atttttcttc gtcacctgat aagataacta
    39361 ccatttctcc aactggaata ccatctaaaa tttcaatatt cccatagagg atgttagcag
    39421 ttacttcaaa ttctttataa atttgattta aaagcggctc atccgtgcta tgcccagcgt
    39481 atttcagttg aactaatttg ctacctactg gtagattttt gacaacttct tgttgctcaa
    39541 tcttaagcat tgcctcatca atcccagtag ctatttttat aaaatcttgt gtaagactct
    39601 ctctaggatg tgaaaaaatg tctaaaactg atccttcttc aatcaattta ccattttgca
    39661 taacagcaac gcgattagca atgtctttga caatttgcat ctcatgtgta atcaaaacaa
    39721 tcgtaagtcc caatttttta ttcagatctt ggagaagggc aagtatttgt tttgttgttt
    39781 tagggtcaag tgcagaggta gactcatctg aaattaagat tttaggatcg ttagccaatg
    39841 cacgcgcaat ggcaacacgc tgtttttgcc ctcctgataa ttgtgaagga tagttttgag
    39901 cacgatcgga taatccaaca agttctaata atttagcaac cttagctgct ttcgcctctt
    39961 ttgataaacc tgaatgttta agagcaaaag ctacattctg ttctgctgtc aactgtgaca
    40021 ttaggttaaa atgttgaaaa atcattccaa tttcacggcg ctgttcgcgt aattgtgtgc
    40081 tagtcagtgt caccttgttg tcgtaaataa cttggtcatc aatagtaatt ttacctgctg
    40141 aaggttcttg tagcaaatta ataacacgaa caagggtaga ttttccggct cctgaataac
    40201 cgacaattcc atatatatca ccttgattaa tatggatagt aacatcttta acagcattaa
    40261 tctcacgttt cttttggtgg aaagtaacat caatgttgtc taatttaata attggttctt
    40321 tactcatatg attctattaa agcctccact aattcaatat gggtgtaata atcagcaata
    40381 ctaacatttt catcaacgcc atggtctcga ctattggcat gtcctatgcc aatagctgca
    40441 attggcgctc ttagagcttg atgaactaaa tacattggtc ctgtacctgg tgaggtaggt
    40501 aacagagata tcccttctgg ataaaattgc tcagctaaat caacaacctt taggatagat
    40561 ggcgctgaca tatcactacg ataagacatt tcaccaaggg tataggttag ctcaatgtct
    40621 ttaaagccat tctctttcaa atgattttgt attgaatcta gtacaccttt cggggttaac
    40681 cctggtacta acctaacctc tgctttacat ttagcataag caggtaaaat tgttttgacg
    40741 ccttcacctt gataacctga ggttatacct tcaatggcta tagaaggttc aaaataaagc
    40801 tttctcaaaa aagttttatg actatctgct aaactgggca aaactaattg gtaagcacct
    40861 tctagcgcct ttgcagatct ataagaatat ttttcaacta attctaactc tctcttagta
    40921 gggggaataa ctttatcata aataccttct accaatatat gacctttgtt atcccttaac
    40981 gtattgaggg cttgaattaa ataccatgtc gaggaatcaa caaccccacc aaaggatgaa
    41041 tgaatatcaa catccgctga acttactgat aaatcaaaag tgacaatgcc tttatttcca
    41101 ccagcaattt ccaactgtcc tttaggattt ttagggccat cttcccacac gatcaaatca
    41161 gccccttgta gctgctcttg atacttttct aaataataat caaggcctac tgaagctgat
    41221 tcctctgctc cttccacaat aaaagtgata tctagaggaa gttccccttt gtgtcttgat
    41281 aaatattttt taactgctga taaacgtgcg gtaatgtgac ctttatcatc atctacacca
    41341 cgtccataca tcttaccata tcgaagtgaa agtgtaaaag ggtcctcagt ccactgttct
    41401 acctcatctg ctggtacagt atcataatga ttgtaaaaaa taatacgctt agcatctact
    41461 tttgatgatt tgaaatttgc cacgataaag ggagcgctat agctgtcatc tataatgact
    41521 tctgcgccag cttttctgaa aacttctcca agatagcttg ataagtcatt taaaccaact
    41581 ttctgagcaa aaattgactt aattgcaatt aaatctctta aaatattgat atcatcctga
    41641 acaatagtat cagatagaaa cttttgaatt tgattttctt cgtttgttaa agtcatcttt
    41701 catccacctt ccttcaaaaa caatccaaga gcctggcaca ttgtattcgt cccaggccct
    41761 ttatggctat atggctgata agttgtatcc tattaggatt ccctgttaaa cttatacagt
    41821 caattcacgt taccattgtg gaatatcagc tgaagtatct ttgataactt ttttcacttc
    41881 atctgtgtga taagcatcca agatagcttg gatagcttta gcgttctttt gctttttcca
    41941 atttttacgt cccgcaatga tattaatcca ttgttttgaa tttttatctg atttctcaac
    42001 aaagatagca tctgaaggtt ttaaattagc ttgctcaatg tatgtattat taataatagc
    42061 tgcatctaca tctttgagtg cacgtggtgt ttgactcgca tctaactcct gaatattaat
    42121 atccttttta ttagatgtga tattagcaac tgttgcaacc ttcttaccag aaacattcaa
    42181 tttgattaaa cctgctgact gaaggacata caatgcacgg ctaccatttg ttgcatcatt
    42241 tggaattgca atagtggctc cttttttcaa ttttttaaga gattttacct tctcagaata
    42301 gatacgaatt ggagctaagt aagtcttttc aagtggaatt aagtttttct tattttcctt
    42361 attccagttt tctaagaaat tgtaatgttg aaaggcatta atatccacat ccttattggc
    42421 tgtcgcttga tttggttgtg tataatctgt aaattctgta aatttgattt tagctttatc
    42481 acctactagc ttttcaattt tatcccaacg tgctttttca gtgtcagaaa aggtcataac
    42541 accaacttta acaactttac ttgaagttga gcttgctgaa acttcttgat tactaaatgt
    42601 gactcctaaa agtgttgtta aggctaatcc taagacaata tgttttaact tcataataaa
    42661 ttccctcaat ttttgtgata aagatattat cccataggaa gttattagaa tcaaattgtt
    42721 ataaactgtc tcttgttata gttttaaact atataacaaa aaactaagga ttgacatctc
    42781 cttagttttt attactactt ctccttagga gtaacgagat ctttctcatt tggtacgtaa
    42841 tcacctccaa ggtattcttt agctattttt gaaagtgtcc catctttacg taattgttta
    42901 aggcgattat tcacatactt ttgcaagtct ttttgatcct gaccaaagat gaaataaata
    42961 taaggctgat ctctcatagt caaagggatg gtttttaaat tcgtgagacc ttgattcttt
    43021 atgatagcgt taacagttgg tccgtcaaaa agtttaaaat cagcttttcc atcactcaaa
    43081 ttcgttagaa tctgtgtaat attttcatta gtatatttta gagtaacagg atttttctga
    43141 tgctctttat tgaaattttc taattgcttg gcagttgtcg ttccttggac aacctgtgtt
    43201 ttatgaccac taatatcatt ataagctttg atattactat tcttaggaac tgctaaaact
    43261 gaaggagtag agcctattgg gtatgaaaat aaatattttt gagatctctc tgatgaataa
    43321 gaaatattat ttccacccat ttgaaacttt cctgaatcaa tgccggtaaa taccgatgac
    43381 cattctgttt ttttaaaagt gactttatag ttatctgaac ctttaaaaac agctttggct
    43441 acttcaatat caaagccagt cagtttccca tccttcacat aagaaaaagg tgccgttgtt
    43501 ccaacagttg caaaattaac ttcttttttg cccgtcttag tagtatgaga ttttgagcta
    43561 caagcagcta gagacgctac tgtcactaaa ataattcctg ttacacttaa cacttttttt
    43621 attgtcatct tttttctcct ctttaattgt acacgacatc ataacatctt taagttttta
    43681 actaatactt atcttctatc gtctgagagt ttttgctatt atatggatgc tacgtttcat
    43741 taacaaaata accaaataag gtttggtttt caatttggtg aagcattaat tctggatgcc
    43801 attgaacacc tataatacat tgttctgttc gactttcata agcttctact gtcccatctc
    43861 taggatctct agcactaact atcagacctt cagctaacag tttaacactt tgtctatgaa
    43921 gagaattgat cttaaaatca cctgatataa agccttctag aaaactccct ttttccacat
    43981 ttaaataatg agcaaatcca atgtaaggct cttgatagtg attatcaata gattgattaa
    44041 gtgtaccacc aagcgccaca tttactaact gcatgccacg gcaaatcgca aagattggtt
    44101 tattttgttt caacgcttct tcgactaatg caaattcaaa aatatcacgc gcaagtgaat
    44161 aatcatcact ctcaattatc ttctcttcac cataatagga tggtaaaaca ttttggccac
    44221 cagatatgat cagcttatct atcatctcaa cataagcttt ggcactttct gcttcactta
    44281 ttggtaggat aactggtaac ccgcctgcgt ttttaactcc ttccgaaata tgcctagata
    44341 ccgaatcata ataataacct ggaatatctg acatttctct ttcatttcct gtaataccaa
    44401 taataggctt tgttaacatt ttgctccctc atcaaccaat aactgtctaa taattatcat
    44461 tttacaccta aaaaaacttt ttgaataata ttttttacct atcacttctt taacaatacc
    44521 tatcaactct cacaaaaata aaaaccatag tattaatact acggttttat tttttagaaa
    44581 tctgctacat tatgataaac tttttgaaca tcatcatctg attcgagtgc gtcaataagc
    44641 ttttcaaaag tttcaaggtc atcaccttcc aatactactt ctgattgagg aatcatttca
    44701 agttcagtaa cttggaattc ttctacacca ttatcgcgaa gtgcttggat acctttatga
    44761 agatctgttg gggcggtata aactgttatt gttccctctt ctgcttcaac atcatctacg
    44821 tctacatccg cttcaagtaa ttgttcgaag acagtgtcag catcatcacc agcaaaaacg
    44881 atgacacctt ttttatcaaa taagtaggat accgatcctg aagctcccat attgccaccg
    44941 ttcttaccgt aagcagtacg tacatttgct gccgtacggt taacatttga tgtcaaagta
    45001 tccacaataa tcattgaacc atttggacca aaaccttcat agcgtccctc tacgaaagtt
    45061 tcatctgtgt ttcctttggc tttatcaatc gctttatcaa taacatgctt tggaacttgt
    45121 gcttgcttag cacgatccaa aacgaatttt agagctgagt ttgactcggg gtctggttca
    45181 ccttgctttg cagcaacata tatttcaaca ccgaatttag cgtatacttt tgagttagca
    45241 ccatctttag cagtcttttt ggcaacaata ttggcccact tacgtcccat tggaaaatct
    45301 cctttgattt catttttaat aacctgctgg ctcatattgg caagcaatct taattttaac
    45361 tctattatta tagcactttg atagcaattg gtaaaatgga aattctatca cggtatgcag
    45421 tagaattttt tagaattgtc aattttctat taaggagaac ctaccgagtt ccttttataa
    45481 aaagactgaa caaattttcc tttgcccagc cttgacagat tcggtgttat cctgtatctt
    45541 acgataaaga ctaagtaagg gaacgttgta attggactta ctcggtaaag cgtagtggtt
    45601 accataactg cgcttctttt attcgattaa ttcaatagaa ttgtagcaca ctacaaaacg
    45661 aataacgagc tttttaaaga aaaatataag aaaagccctt ttaaaggtgc ttttcttata
    45721 tttgtttcaa atcaatcaat ttctttattt ctttaatttc gacatcctta aaaggaaccg
    45781 ttttttcaaa ctcgcctcta ttaacttgtt taaatcctag atctctgtca ttaccaattt
    45841 gtacatcaat agtgcataac tcaactattt tctctctatt ttttccttct ctaataaaaa
    45901 attgtgtaaa tatatggtca caatagaagc aacatctttc cacttgatac acctcactaa
    45961 tttcagtttg atcaaccggt cgtatgtgat aatattgata tttgctgaaa cccttttgcc
    46021 actttctact ccataggaaa aatttatcct gcttactata taacgacacc tcctttcctt
    46081 ggaaaactgt ctctcccaca tgagtcgcac catcaagtgg tgcatctttc tcatcaaacg
    46141 aaatcttttt cttagacaaa atcgacatta tcaactgtgc agaacctatt gcataaaaaa
    46201 ttgttcctac aataaatact agtaaaatta ttgttagtac aatgattcca aaaactttct
    46261 gtgcatccat tgaggaatac ccagacaaat tatataccaa tgtatatcca cctacagcag
    46321 ttattgatag gcaaataaaa aataatatca acacaccgta cgaagaatcc atgatcttac
    46381 atactaactt ttctgataga aatttccata caaggcgcac aacacttccc tccctaaata
    46441 tattaatata atagctatac acattaaagt gaccatattc ggatattgat catctagtct
    46501 catgtcatca aaaatcaaaa actcaataaa attctatgct acatggctat aattgaagta
    46561 agctgagtta ttttagattc gtataacatt acagtagata tatatattgc aacaagcaaa
    46621 gccctaaaaa atagtaaacc tttgtgattt actatttcta aaaatattct attcaattaa
    46681 tcaagctaga agctatcgtt ctactttcta atgcaatttt ctgtgaaagt tttcttcaac
    46741 tcccgttcag tcgtaagtat taacacaatt aaaatagtta ttcgtataga aactacaatt
    46801 aagctaataa tacctatttc atcatctgtg cttctataaa atagaatatg aataataatt
    46861 aatagccaca gtaatcatgt ataaaactcc atgtacctat tttttcattg ataaagtagt
    46921 acgatatccc attaatctat taattttttc aggtagatga ttccacatca ttcgtccgtc
    46981 aataatcatt gtaactgaaa ctagtaaatc ataataaaat ataaatcact aaaaccattt
    47041 aaaaaattct ccctccaaac actgatttat gaatatggat ttaactatta aatattttat
    47101 ccatattatg ataaaacaat ggtaagttca atttttcctg ttttccagca tcatagatac
    47161 atttacaacc atacataata tcttttacat cattgtgcaa cgtcataatt ctactcgtaa
    47221 gttcatttct tgcgaacatt cgtttcataa ttattcctgc aatagtagaa ggtattttat
    47281 aaggaaaaag ttcattttta tataatctta aatcgacccc tctatttttt actactttaa
    47341 ttgtttctct tattgatttt accgcgatag acaatccatg attatctctc ataagattaa
    47401 ccgcaagttg ttttggatca tcaatttttc ctgcccttgc tgcagtagaa gtaacacctg
    47461 catttattgc catatgaatc catatccact ctatcatatc ataaggattt tctactttta
    47521 tgtcggcaga ttcgagaaat tctaacaact ctttatagtt tgtgatagtt gctttttctt
    47581 cactttcaag cattatatgg tcaaacagaa cacaattcaa ctttttctgc ttcaaattcc
    47641 cacctgctgt cggaaatcca atgatatatt taaaatcacc aaccattttt tcgatttctt
    47701 cacggctgtt ccaaaagtta caaaataata acaaacttcc tgtaatatta ttttgtgaaa
    47761 gtgttgctat ggcatctttt atcctcccgc ctgcaacact taaaaggata aagtcataat
    47821 ttgtatttgg ttttgcaaga ttcacagtat atatatctgt tttttcttct cccttattgt
    47881 tatatcgccc atctaacatg gttatatcca gctttaatgg aacatcttgc tttttacttt
    47941 ctctaactaa atgttcaacc tgatgtcctg ctttttgaaa gatatatcca taagttgtgc
    48001 ctattacgcc taatcctaaa atacatattt tcatttaata atcatctcct taagtttcgc
    48061 ttccagttta ttttactcga taatttttta tattatatca tattttttta cttagtttta
    48121 tagcctaact catacttcct tacctgtatt agatcactgt tttcttacaa taatttttct
    48181 atgctattcc taacactttt agcttgtgct tcttcttttc gtaggtctat tatttgagaa
    48241 tatggactac gctcctgttt aaatgttgac tgacttgact ttaaaaattc tgagatttca
    48301 gctaactgaa agataatatc atcattcaaa taaacatgtt ttctatttcg aatttctgaa
//
LOCUS       DATRUK010000008        48027 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.008, whole genome shotgun sequence.
ACCESSION   DATRUK010000008 DATRUK010000000
VERSION     DATRUK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48027
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.008"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..166)
                     /locus_tag="VBE34_000388"
     CDS             complement(<1..166)
                     /locus_tag="VBE34_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEO7263138.1"
                     /translation="MVSSDNTNLKFLKAFSELLKMRSFEQIKVSDLAKKARLSRRSFY
                     NHYNSKEDFLR"
     gene            349..681
                     /locus_tag="VBE34_000389"
     CDS             349..681
                     /locus_tag="VBE34_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEO7263139.1"
                     /translation="MLRKYKQRNYNVAHTKQRFSIKKFKFGVASVLIGLTFLGMSSHS
                     VLADESSIMSTEVPQTAGVKPEVTVTQPTSDVTSLATDTSTSAVLSTDASHSNPTESA
                     EHAVGLEC"
     gene            complement(802..1946)
                     /locus_tag="VBE34_000390"
     CDS             complement(join(802..1712,1712..1946))
                     /locus_tag="VBE34_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag5 family transposase"
                     /protein_id="HEO7263140.1"
                     /translation="MSTFKRYDEEFKQSLVNLYQTGKTQSELCKDYRVSTSALAKWIK
                     QYSQFKLEDNSVLTAKQIQELQKRNAQLEEENLIPKKSKCHIHAKLKVRLLAVYRLRF
                     EHATTTLCRVLRVNRSTYYKFLKHKPSKRELDNQIYKKQILEIYTKANKRLGVKSIKV
                     ILQRDYDTKISEGRIYHLMKNMALPKMATVKPKTALKKTQKTYPQNLLNQKFYPDKPN
                     QVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHRMDAKLACNTLELALNKRKI
                     EGTLLFHSDQGSQFKAREFRKIIDDNNIMHSFSKPGYPYDNAVTEAFFKYLKHRQINR
                     KHYQNIKQVQLDCFEYIENFYNNYNPHTANLGLTPNQKEENYFNAIK"
     gene            2079..2216
                     /locus_tag="VBE34_000391"
     CDS             2079..2216
                     /locus_tag="VBE34_000391"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263141.1"
                     /translation="MIIARENHQVTHEHVSILLTCVQELIVEVNQTQPLSREFREKYM
                     G"
     gene            2226..2481
                     /gene="mobC"
                     /locus_tag="VBE34_000392"
                     /pseudo
     CDS             2226..2481
                     /gene="mobC"
                     /locus_tag="VBE34_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
     gene            complement(2456..2951)
                     /locus_tag="VBE34_000393"
                     /pseudo
     CDS             complement(2456..2951)
                     /locus_tag="VBE34_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
     gene            complement(3145..4353)
                     /locus_tag="VBE34_000394"
     CDS             complement(3145..4353)
                     /locus_tag="VBE34_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO7263142.1"
                     /translation="MVTFDFKHVQTLHTISQLPISVMSRDKELVQLYSNEDYLLPYYQ
                     FLKHLAIPYNQDITVYEGLFEESFLIFPVCQYLIAIGPFYPYSPDINRQEQLSSRFLE
                     QFSHRSKKEILSYIKLVPCFPTTSIRSLLVSIDAFFQTQFEASCQQVINHLLEESEQI
                     VADPDIVLHLKHTKKNSFQLPTVLNHLNHIIDLVKLGNTQLLKQEINRLPSSSVTSSS
                     IPALRAEKNLTVVYLTKLLELSFEENTDVAKSYALVKHYMALNEEAPDLIDVSRIRCA
                     ALIDFSESLTNKSISDKQQMYNSILHYVDNHLYSKLKVSDIANYLYISDSHLRSVFKK
                     YSDISLQSYILKAKIKEGQLLLQRGVPIGEVAKLLHFYDTTHFLKTFKKYVGISSNEY
                     LTKYRETSCQ"
     gene            4734..6398
                     /locus_tag="VBE34_000395"
     CDS             4734..6398
                     /locus_tag="VBE34_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO7263143.1"
                     /translation="MKLSKKLLFSAAVLTMVAGSTVEPVAQFATGMSIVRAAEVSQER
                     PAKTTVNIYKLQADSYKSEITSNGGIENKDGEVISNYAKLGDNVKGLQGVQFKRYKVK
                     TDISVDELKKLTTVEAADAKVGTILEEGVSLPQKTNAQGLVVDALDSKSNVRYLYVED
                     LKNSPSNITKAYAVPFVLELPVANSTGTGFLSEINIYPKNVVTDEPKTDKDVKKLGQD
                     DAGYTIGEEFKWFLKSTIPANLGDYEKFEITDKFADGLTYKSVGKIKIGSKTLNRDEH
                     YTIDEPTVDNQNTLKITFKPEKFKEIAELLKGMTLVKNQDALDKATANTDDAAFLEIP
                     VASTINEKAVLGKAIENTFELQYDHTPDKADNPKPSNPPRKPEVHTGGKRFVKKDSTE
                     TQTLGGAEFDLLASDGTAVKWTDALIKANTNKNYIAGEAVTGQPIKLKSHTDGTFEIK
                     GLAYAVDANAEGTAVTYKLKETKAPEGYVIPDKEIEFTVSQTSYNTKPTDITVDSADA
                     TPDTIKNNKRPSIPNTGGIGTAIFVAIGAAVMAFAVKGMKRRTKDN"
     gene            6487..7410
                     /locus_tag="VBE34_000396"
     CDS             6487..7410
                     /locus_tag="VBE34_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7263144.1"
                     /translation="MKQTLKLMFSFLLMLGTMFGISQTVLAQETHQLTIVHLEARDID
                     RPNPQLEIAPKEGTPIEGVLYQLYQLKSTEDGDLLAHWNSLTITELKKQAQQVFEATT
                     NQQGKATFNQLPDGIYYGLAVKAGEKNRNVSAFLVDLSEDKVIYPKIIWSTGELDLLK
                     VGVDGDTKKPLAGVVFELYEKNGRTPIRVKNGVHSQDIDAAKHLETDSSGHIRISGLI
                     HGDYVLKEIETQSGYQIGQAETAVTIEKSKTVTVTIENKKVPTPKVPSRGGLIPKTGE
                     QQAMALVIIGGILIALALRLLSKHRKHQNKD"
     gene            7412..8329
                     /gene="srtC1"
                     /locus_tag="VBE34_000397"
     CDS             7412..8329
                     /gene="srtC1"
                     /locus_tag="VBE34_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC1"
                     /protein_id="HEO7263145.1"
                     /translation="MGQKSKISLATNIRIWIFRLIFLAGFLVLAFPIVSQVMYFQASH
                     ANINAFKEAVTKIDRVEINRRLELAYAYNASIAGAKTNGEYPALKDPYSAEQKQAGVV
                     EYARMLEVKEQIGHVIIPRINQDIPIYAGSAEENLQRGVGHLEGTSLPVGGESTHAVL
                     TAHRGLPTAKLFTNLDKVTVGDRFYIEHIGGKIAYQVDQIKVIAPDQLEDLYVIQGED
                     HVTLLTCTPYMINSHRLLVRGKRIPYVEKTVQKDSKTFRQQQYLTYAMWVVVGLILLS
                     LLIWFKKTKQKKRRKNEKAASQNSHNNSK"
     gene            8286..9137
                     /gene="srtC2"
                     /locus_tag="VBE34_000398"
     CDS             8286..9137
                     /gene="srtC2"
                     /locus_tag="VBE34_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC2"
                     /protein_id="HEO7263146.1"
                     /translation="MKKRLVKIVTIIRNNKIRTLIFVMGSLILLFPIVSQVSYYLASH
                     QNINQFKREVAKIDTNTVERRIALANAYNETLSRNPLLIDPFTSKQKEGLREYARMLE
                     VHEQIGHVAIPSIGVDIPIYAGTSETVLQKGSGHLEGTSLPVGGLSTHSVLTAHRGLP
                     TARLFTDLNKVKKGQIFYVTNIKETLAYKVVSIKVVDPTALSEVKIVNGKDYITLLTC
                     TPYMINSHRLLVKGERIPYDSTEAEKHKEQTVQDYRLSLVLKILLVLLIGLFIVIMMR
                     RWMQHRQ"
     gene            9219..11891
                     /locus_tag="VBE34_000399"
     CDS             9219..11891
                     /locus_tag="VBE34_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEO7263147.1"
                     /translation="MKKRQKIWRGLSVTLLILSQIPFGILVQGETQDTNQALGKVIVK
                     KTGDNATPLGKATFVLKNDNDKSETSHETVEGSGEATFENIKPGDYTLREETAPIGYK
                     KTDKTWKVKVADNGATIIEGMDADKAEKRKEVLNAQYPKSAIYEDTKENYPLVNVEGS
                     KVGEQYKALNPINGKDGRREIAEGWLSKKITGVNDLDKNKYKIELTVEGKTTVETKEL
                     NQPLDVVVLLDNSNSMNNERANNSQRALKAGEAVEKLIDKITSNKDNRVALVTYASTI
                     FDGTEATVSKGVADQNGKALNDSVSWDYHKTTFTATTHNYSYLNLTNDANEVNILKSR
                     IPKEAEHINGDRTLYQFGATFTQKALMKANEILETQSSNARKKLIFHVTDGVPTMSYA
                     INFNPYISTSYQNQFNSFLNKIPDRSGILQEDFIINGDDYQIVKGDGESFKLFSDRKV
                     PVTGGTTQAAYRVPQNQLSVMSNEGYAINSGYIYLYWRDYNWVYPFDPKTKKVSATKQ
                     IKTHGEPTTLYFNGNIRPKGYDIFTVGIGVNGDPGATPLEAEKFMQSISSKTENYTNV
                     DDTNKIYDELNKYFKTIVEEKHSIVDGNVTDPMGEMIEFQLKNGQSFTHDDYVLVGND
                     GSQLKNGVALGGPNSDGGILKDVTVTYDKTSQTIKINHLNLGSGQKVVLTYDVRLKDN
                     YISNKFYNTNNRTTLSPKSEKEPNTIRDFPIPKIRDVREFPVLTISNQKKMGEVEFIK
                     VNKDKHSESLLGAKFQLQIEKDFSGYKQFVPEGSDVTTKNDGKIYFKALQDGNYKLYE
                     ISSPDGYIEVKTKPVVTFTIQNGEVTNLKADPNANKNQIGYLEGNGKHLITNTPKRPP
                     GVFPKTGGIGTIVYILVGSTFMILTICSFRRKQL"
     gene            11945..12735
                     /locus_tag="VBE34_000400"
                     /pseudo
     CDS             11945..12735
                     /locus_tag="VBE34_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            12984..13589
                     /locus_tag="VBE34_000401"
     CDS             12984..13589
                     /locus_tag="VBE34_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEO7263148.1"
                     /translation="MARDPYSDRSRYLDPKVLSSSFGAFFPADNIKVAWSNNSSTLFT
                     PPINANYTTQIQAIGTTIKSQIPESILTVTDKKSRKFSINKIDEAKEGLVGATFTLSK
                     RTTVAADHQVQGDFIPVSKETTVGRTTLTFDNLKPGFYDLKETKAPNAYVLDPKTYVV
                     VVQNSGKTTIVDEANFKEADYPMADNTSHVECVALLQRSKG"
     gene            14110..14300
                     /locus_tag="VBE34_000402"
                     /pseudo
     CDS             14110..14300
                     /locus_tag="VBE34_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883557.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
     gene            14687..14863
                     /locus_tag="VBE34_000403"
     CDS             14687..14863
                     /locus_tag="VBE34_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263149.1"
                     /translation="MHVGKSYFDYQENALIPQLGFLYSKLTKKIELDNRLSPTEQKLL
                     ITLLLHTKGLIIDM"
     gene            16208..16477
                     /locus_tag="VBE34_000404"
     CDS             16208..16477
                     /locus_tag="VBE34_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263150.1"
                     /translation="MVSLLLLKVKQEELIEFHNVFYREAISSYLETENFEKAKVTEEN
                     YQVFLGIRTYQKSYNYQDHSENLKGNIEVAMIGVENNISDEHIFC"
     gene            16538..17689
                     /locus_tag="VBE34_000405"
     CDS             16538..17689
                     /locus_tag="VBE34_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO7263151.1"
                     /translation="MVRKFLKSLLSLFLIAVIATGISVACFFFIPENKGNITPILLHR
                     FMRKNNVNGMMIVSDNTGKPITISHGINRGEVETDIENNKLFPMASLQKLMTGIIIQR
                     LIDQDVLSEDDRLSQFFPQVKGSNSITIHQLLTHTSGLREKGVKVSPYLKNEREQLQF
                     CLKHYNFVNKKSWYYSNINFSFLTGIATQVTGRTYAELVDDVIKNPLRLDDTQSYQSV
                     VNHDLVSPMRKNGKLNKINIFNQVSTAYGAGDFFTTPLNFWVLMRSFSKGYFFPTDEY
                     TKHQNDAISHYYGGLYMHGRIVNSNGAFFQYSSFGYADLKTHDTMVLFMNNKVYSDAS
                     HVAPKAFEYYQKFMFLNKIFHLVFYFVFSFFLMLLIRHSFRKRRYKKRL"
     gene            17889..18881
                     /locus_tag="VBE34_000406"
                     /pseudo
     CDS             17889..18881
                     /locus_tag="VBE34_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            18884..19702
                     /locus_tag="VBE34_000407"
     CDS             18884..19702
                     /locus_tag="VBE34_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEO7263152.1"
                     /translation="MNRIWRRYKPFISTGIQGLITYRVDFILYRIGDVIGAFVAFYLW
                     KAVFDSSSQSLIQGFQLSDMILYIIMSFVTNLLTRTDSSFMIGDEVKDGSIIMRLLRP
                     VHFAASYLFMEIGSRWLIFLSIGVPFLLVITGVRLFLGTDLIQAIVLVVFYIISIILA
                     FLINFFFNICFGFSAFVFKNLWGSNLLKNSLVAFMSGSLIPLTFFPKIVADILGFLPF
                     SSLIYTPVMIIIGKYDGSQIVQALLLQIFWLIVMVALSQLIWKKVQSHITIQGG"
     gene            19704..20489
                     /locus_tag="VBE34_000408"
     CDS             19704..20489
                     /locus_tag="VBE34_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7263153.1"
                     /translation="MTKYQRMHFIFIKQYMKQIMEYKIDFFVGVLGVFLTQGLNLLFL
                     NVLFQHIPSLEGWTFQQIAFIYGFSLLPKGIDHLFFDNLWALGQRLIRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGFILLSTTVSSISWIVPKVLLFIFIIPFATLIYTS
                     LKIATSSIAFWTKQSGAVIYIFYMFNDFAKYPVAIYNNLLRWIISFVIPFAFTAYYPA
                     AYFLQDRNVYFNIGGVILISLISFMVSLILWHKGVEVYESAGS"
     gene            21114..21419
                     /locus_tag="VBE34_000409"
     CDS             21114..21419
                     /locus_tag="VBE34_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylX protein"
                     /protein_id="HEO7263154.1"
                     /translation="MGRSNILSRCEQCSDNQCGLYKTRLKDKLLLVVQLVGNIRYSEF
                     LTEGGVYHKGETLLEVITLGISYEYKSYGDIKVIKKYCADGSVVDYNMPILLMERIE"
     gene            21419..22267
                     /locus_tag="VBE34_000410"
     CDS             21419..22267
                     /locus_tag="VBE34_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylD"
                     /protein_id="HEO7263155.1"
                     /translation="MKTVIIYGGQGNVQSKHLCQLVKTPKGEILFSKLQATDLESYQL
                     MMDVINHKVSLDNVHASMLANFLYNDWLTNDEKIVSGIGFSAHSAGIFNVLYASGSTS
                     FENIIHFIKRRAQLIENCHVSEELWLLMVENIDMFSKDVLKEQSEKVKLAIITDDRAG
                     VIAMDQVNRSFFDDVMTNKGYFYKLKQLGVKAPYHTDFLNESKEQYKQLIESLDISQN
                     DDYDYIFHCDQLVDEILYQWTNLFNWRAIKETIIEKDAEIIDISPNRFISSQLQKMKK
                     RGRKQL"
     gene            22264..22986
                     /gene="fabG"
                     /locus_tag="VBE34_000411"
     CDS             22264..22986
                     /gene="fabG"
                     /locus_tag="VBE34_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEO7263156.1"
                     /translation="MKVAIVTGGTRGIGRAITKELYKEGYKVIAIYNSNDAKARALQE
                     ELPKLDVYKCNISDAKAVQKLVTKIFREYGGIDCLVNNAGIVRDGFFLMMSKEKWMDV
                     ININIMGLVNMSKAVLKIMKAKRIQGKVINISSTSGIAGQIGQANYSATKGAIISITK
                     TLAKEFASDGITINCVSPGFIETDMTNELQNKEELKEHLIPLKRFGQPEEVAWLVSFL
                     ASEKANYITGKNIVIDGGMIND"
     gene            22979..23284
                     /locus_tag="VBE34_000412"
     CDS             22979..23284
                     /locus_tag="VBE34_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO7263157.1"
                     /translation="MINWAEERKIIKTRQEVVDEIKGILIDSLMLDIDKDLIMNDQPL
                     FGRGLELDSIDALELSIGISTTFGVELNDDDISILSSVNRLADFVIENSEDIDDQAK"
     gene            23268..23744
                     /gene="fabZ"
                     /locus_tag="VBE34_000413"
     CDS             23268..23744
                     /gene="fabZ"
                     /locus_tag="VBE34_000413"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO7263158.1"
                     /translation="MTKLSSEEIIKMLPHRYPFLMVDRVQSYKKGIIECRKNITINEP
                     YFQGHFPQRPIVPGVLMIEMAAQSAALLYILDALDGSLPSLDELTAEKIAEKVGYLAS
                     VKNFKFKKIVTPGDQLNITCKSQSKLGHLLEIQVVIRDENKKEVASGRMLVSENGN"
     gene            23734..24663
                     /locus_tag="VBE34_000414"
     CDS             23734..24663
                     /locus_tag="VBE34_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7263159.1"
                     /translation="MEIKLKNIGKKYGSFEALRDINLIFEEGKFYGLLGPNGAGKTTL
                     FNLLIQNFKQTSGDIKWEVDGKPLSIKDFYRHIGIVFQSNRLDDNLTVEENLISRGAL
                     YGLSKSQVRNRLKDLQTYLDITAIKKQKYGSLSGGQKRKVDIARALLPQPSLLLLDEP
                     TTGLDPQSRRDLWDAIAQLNQQSQMTVVLITHYLEEMSACDVLNVLIEGNIYYSGDIK
                     SFIEQHSTTNLNVVLKPEKSLDQLSIADFVNKCQVLSEREIVFKDISVEEMMQVISDN
                     QDKSIIETFNVEYSNLEAAYLNLLREKERKNNV"
     gene            24656..25534
                     /locus_tag="VBE34_000415"
     CDS             24656..25534
                     /locus_tag="VBE34_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7263160.1"
                     /translation="MFELISRNRKVYTRDRLAFFMSFLSVIILILVYQIFLGKIQLDA
                     IKAALRSNTVSSDTIHMVNYWLIAGLTTIISMTSTLGAFGVMVSDKEKKLNEDFKVSP
                     ISNIKIELSYAIFAVLFGIVMTMFSCIFAIGIFNGFNSLLEYSAMDYLTILGIVSLGT
                     VLSAAMILPILAFIHTSSAFTTLSTIVGTFIGFISGVYLSIGSVGKALQQVMTWFPLT
                     QINSLLKQVLMKGSIAKVFDKANEATVSNYKESYGVVLRNADGERLSNHFMLIYIIAL
                     ILILLAIHFIIKKVKK"
     gene            25531..27534
                     /locus_tag="VBE34_000416"
     CDS             25531..27534
                     /locus_tag="VBE34_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylE protein"
                     /protein_id="HEO7263161.1"
                     /translation="MKDDNKLKISEASLEDYSEVVHLFNRNHVYQFPDGRPLTVDDLD
                     LTLKVKEVTHLFLLKNHGVLIGTSAFFKFITYGCLDWNSSFSGFLLIDSKSRSGQAIT
                     YLYKTILKKITKLKFSNIYTEISNYNKPSLALSKLNGFKEYDKTYEDILHCRSLRSHL
                     PKILNTFRISNYYGKTYDISTFQIMEEIENPLEEETEIRTKVSDEEILFKAEDSASLP
                     YYLKMSLFQMEIAKLDNRYVLQVDFLSEQVKRVRVKTGKYHLANLTRAHPSLTLSRFA
                     NYYYIQATVETLYGNIDVQLERRKKHYRDATICLKRTFQGYDLLISPNGSLIFEKQKR
                     KILEDSFLIFSQPLDKKLVVKEEENHITIKCFYQGALIEKIMTFTSDEEITCVYKCNQ
                     KAKEMFPKLLKQTFKLHCQEQLIRDSEGYLVNVPGSYPIEHDDFLRADKFEDRQFHYY
                     LPNEDKMISYSPPGKASNQMQFRPLCLIDTDSLSFPLTYHFRISQASSEEALINLKKQ
                     PIWDSNYQASATDLLKHISNLTLEEEKDYGIKRMIANRKHYPSHKLVLAYNQIVLPKN
                     EIPRDSELYSISFDYRIRGKFVQIRQGEHVKYDNKSYVLENSQQLVLYVASDDKYILI
                     SAKNGIFYSYKENNHLKIRCMFKRNSPYATNVSITEYRKCEEK"
     gene            27531..28484
                     /locus_tag="VBE34_000417"
     CDS             27531..28484
                     /locus_tag="VBE34_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEO7263162.1"
                     /translation="MNTIYNTLRTDKGYKVYEGYLYEITGEECEEALDLVIPKNIVFA
                     DTDTCGYTFLLNEDGTVYDDVTFYKFDDKYWLASHKALDSYLDNINFDYTVTDISDEY
                     KMLQIEGRYSGEIAQSFYEYDISTLNFRTLIEMTYKGEKGYLARFGFSGEFGYQFFLP
                     SSIFATFVSDVCEGIAEYGDELDRYLRFEVGQPITDIYQQEEYSLYEIGYSWNLDFTK
                     EEFRGRDSLLEHIRSATVKSVGFSTKEKLASGTPVLFDDQIVGKIFWIADEKDSSENY
                     LGLMIVNQTYAHSGVTFVTEDGQILKTQSSPYCIPESWNKE"
     gene            28481..30676
                     /locus_tag="VBE34_000418"
     CDS             28481..30676
                     /locus_tag="VBE34_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEO7263163.1"
                     /translation="MSVYVSGIGIISSLGKNYSEHKQHLFDLKEGISKHLYKNHDSIL
                     ESYTGSITSDPEVPEQYKDETRNFKFAFTAFEEALASSGVNLKAYHNIAVCLGTSLGG
                     KSAGQNALYQFEEGERQVDASLLEKASVYHIADELMAYHDIVGASYVISTACSASNNA
                     VILGTQLLQDGDCDLAICGGCDELSDISLAGFTSLGAINTEMACQPYSSGKGINLGEG
                     AGFVVLVKDQSLAKYGKIIGGLITSDGYHITAPKPTGEGAAQIAKQLVTQAGIDYSEI
                     DYINGHGTGTQANDKMEKNMYGKFFPTTTLISSTKGQTGHTLGAAGIIELINCLAAIE
                     EQTVPATKNEIGIEGFPENFVYHQKREYPIRNALNFSFDFGGNNSGVLLSSLDSPLET
                     LPARENLKMAILSSVASISKNESLSITYEKVASNFNDFEALRFKGARPPKTVNPAQFR
                     KMDDFSKMVAVTTAQALIESNINLKKQDTSKVGIVFTTLSGPVEVVEGIEKQITTEGY
                     AHVSASRFPFTVMNAAAGMLSIIFKITGPLSVISTNSGALDGIQYAKEMMRNDNLDYV
                     ILVSANQWTDMSFMWWQQLNYDSQMFVGSDYCSAQVLSRQALDNSPIILGSKQLKYSH
                     KTFTDVMTIFDAALQNLLSDLGLTIKDIKGFVWNERKKAVSSDYDFLANLSEYYNMPN
                     LASGQFGFSSNGAGEELDYTVNESIEKGYYLVLSYSIFGGISFAIIEKR"
     gene            30681..31892
                     /locus_tag="VBE34_000419"
     CDS             30681..31892
                     /locus_tag="VBE34_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylJ protein"
                     /protein_id="HEO7263164.1"
                     /translation="MSKFLFAPFMMNLGESQRLSKLANCLYEQGHDIHILGDNYYPFL
                     FNNDAYRYHHCVEDKSVYNSKRYDAFFSLSTDFNFLSEEEIERICSIERDLLRQEKFD
                     AVLTGYRLSIVTSCRLESIPLIWIISGATHISEIVENSEGILPNGKISKASKPQTKDF
                     IKRVITTYSTNVKTWNNYIKKYGGKPFNNALELFTGDLNLVTDYSLFYEFDKDSSYKT
                     IGPILIDNVGFSKCSQINQDNKTVLLSFGTSFKRDWVESFLKTLPRHYHYLLTSCGEA
                     INIPGSYIEIVDFIDFKTIDKEIYFAIIHGGQGTVYAMAAQGIPFIGIPFFNEQFWNI
                     KKFSKEKCAYLVKKPDVREIRKAISCLERDYDIYKSYMIRLSQGIKEEAEASLQKAEA
                     EIESFIRKRQS"
     gene            31861..32436
                     /locus_tag="VBE34_000420"
     CDS             31861..32436
                     /locus_tag="VBE34_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylK protein"
                     /protein_id="HEO7263165.1"
                     /translation="MNPLSEKDKVNYIMIYKNRQSTLENNLSGDRLMRYQQANRQVSK
                     DKLLTGSYFVYQCLKKLGFTNKICQESFSSVSSYLIGLPKGKISYSNSGDYHILTYAP
                     SGSTGVDIEKYKDRSEQTYQNYLGESVSSDMDSKLLFYKAWLQKEISYKCGKSIDITY
                     QTMIDGYIYGYAFDNTFKVEAVYLKELLPDN"
     gene            32779..33120
                     /locus_tag="VBE34_000421"
     CDS             32779..33120
                     /locus_tag="VBE34_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263166.1"
                     /translation="MSEFLSAKPIGHNDSSNTILRIIENTKYIHHYDGKVVVEIPPQK
                     EPIKVFFMDSRLHYLEFSDICRYVTVVLKNSTVDNITNLGLDNHIVYDNGDDMVDPLP
                     SIQHGLFLNDD"
     gene            33154..33669
                     /locus_tag="VBE34_000422"
     CDS             33154..33669
                     /locus_tag="VBE34_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263167.1"
                     /translation="MTFYKKTDKFGFRKSKVCRSLCGALLGTVAVVSLATASTEIHAD
                     EATTSPTTVTKVPQPVQADTTALNTSKTHSTQATTTPVEAKENKVVKSETVQSESRVM
                     PRDKVVERPETVKASVNSDVSQPITTTPPTINEKTVEIPNLAQDTKKVAPKVTVTPEV
                     VNQPKQLPKYE"
     gene            33721..36378
                     /locus_tag="VBE34_000423"
     CDS             33721..36378
                     /locus_tag="VBE34_000423"
                     /inference="COORDINATES: protein
                     motif:HMM:NF017709.1,HMM:NF018035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO7263168.1"
                     /translation="MTKPQGAVAEKATPAVPKPQKVRVIVEFNKESILDYATEQKKTV
                     AQLNQADVEKKLQSIKQEQDKVLKNIEKSVHFDSSKVKRYDAIINGVALDIQAQEIEK
                     LKTIADVRRVYVSQEYVQTKPLLSSSGQLIGLPEVWNNSQYKGEGTVVAVIDSGVDFK
                     HQALKIKEPNRAKYNKTSIEKLIHEKNLKGKFYSEKVPYGYNYYDYNDNLKDSYGVMH
                     GMHVTGIVGANDDNQKLYGVAPNAQILAMKVFSDDQQNPTTFTDVWLKALDDAILLKA
                     DVVNMSLGTPAGFVHEGKDYPELEVIARACKAGIVIAVAAGNEGNITDGNTYGVKPLA
                     ENYDTALIANPALDDNTLAVASMENLKKHAHVLKFKDKKSGTEVTEVINLHVAPNASK
                     TIIGLAVDLGAEAPSELSKHFDLSGKIAMLEIPEDNKSNGFLEKVQAITKLNPAAILL
                     YNNAKVKDDLGSQLLVESEAAKFNIARITRSTYNNIKNNSNKIITILTERQAIDNSLA
                     GQLSSYSSWGPTPDLRLKPEITAPGGHIFSTVEDNQYADKSGTSMAAPQVAGAAAVLK
                     QYITDKKIPVDNAADFIKLLLMNTAQPIINKQSKDGKTPYFVRQQGSGAMNLAKALVT
                     TVVATVTGTNDNNADGKLELRELKEKKFKARILLRNFGKTNKTYIISSEAIADPVDEK
                     GFRTQNSEHLVSKKADAVTRKVTVEAGKTLAVDLDVDYSDAEALTRNNFLEGYLNLKD
                     TEGVADLHLPFLGFYGSWTEQKAIDAFEGISEIGNGDKKRRVQFYVNKETNKTSSTFT
                     TNGMLSLPIYNNTVFFSPNSPFYDKAGVRIAALRNMEYVQYSIIDPDTNKEVRVLGRS
                     HDVRKLYRLDYRNSFAMMPDSIWDGKIKD"
     gene            36424..39742
                     /locus_tag="VBE34_000424"
                     /pseudo
     CDS             36424..39742
                     /locus_tag="VBE34_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060267.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            39926..41011
                     /locus_tag="VBE34_000425"
     CDS             39926..41011
                     /locus_tag="VBE34_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M13"
                     /protein_id="HEO7263169.1"
                     /translation="MQRKSYLKSMSVLTLTACLISGYVVKDIAMLHAVSASEKKANNV
                     SPRENLYRAVNDNWLANTKLKQGQTSVNSFSEIEDKLKQLLVSDMAKMASGKIETTND
                     EQKKMVAYYKQGMDFKTRDKNGLKPLKPVLQKLEAVSSMKDFQSLAHDFVMSGFVLPF
                     GLTVETNARDNSQKQLVLRQAPALLESPDQYKKGNKEGEAKLSAYRTSAMALLKQAGK
                     SNIEDRKLVKQAIAFDRLLSEKTQVDQSKITAESETAAGRYNPESMETVHNYAKEFDF
                     KELIEKLVGPTNKAVNVEDKTYFKQVNDVINSKQLANMKAWMMISMLVDQSDFLGEQN
                     RQAASAFKNVASGLTQIELKEKMLTPN"
     gene            41032..41850
                     /locus_tag="VBE34_000426"
     CDS             41032..41850
                     /locus_tag="VBE34_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEO7263170.1"
                     /translation="MGDYYGKKYFGEAAKKDVEHMAKKIINVYKTRLKNNTWLSENTK
                     AMAIKKLDNMRLMIGYPEDYPDLYRQYQFDSKASFFENNDNYRKLSNKKTFEEFNQSN
                     QREHWQMSANAVNAYNDPNTNSIVFPAAIFQSPLYDKTKTVSQNYGAIGAIIGHEISH
                     SFDINGMKYDEKGNLHDWWTKEDLKHYKKKTQAMIDQWDGLKADGGKVDGKLTLAENI
                     ADNGGVMASLEALKTEKIQTIKNFLNHGQVFGVKKQPKNKVSPQFSQMFMHHMN"
     gene            41847..41957
                     /locus_tag="VBE34_000427"
     CDS             41847..41957
                     /locus_tag="VBE34_000427"
                     /inference="COORDINATES: protein motif:HMM:NF013590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263171.1"
                     /translation="MRANIPVRNFQEFYDAFGVKKGDSMYLKPEKRLTLW"
     gene            41989..43020
                     /locus_tag="VBE34_000428"
     CDS             41989..43020
                     /locus_tag="VBE34_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7263172.1"
                     /translation="MKNITKLSTVALSLLLCTACAASNTSTSKTQSHHPKQTKLTDKQ
                     KEEPKNKEAADQEMHPQGAVDLTKYKAKPVKDYGKKIDVGDGKKMNIYETGQGKIPIV
                     FIPGQAEISPRYAYKNLIERLSKKYKIYTVEPLGYGLSDIPTKPRTLENITKEIHTGL
                     NKIGVKNFYLAAHSLGGMYSLNYAKNYPEEVRGFIGMDTSTPWMEGEQKTKYDPESAK
                     WAMKLPDVDDKTNEQYLSIAKKINGNKSQRDEDERLGDNLGKLKNVKFPKGMPTKYFL
                     ADQSVEGIKLRQADFKGLKNWDQQHIDLSEDPKDASVEILKGSHNIFQTQYEHMAKAI
                     DGFIQSHQK"
     gene            43055..44054
                     /locus_tag="VBE34_000429"
                     /pseudo
     CDS             43055..44054
                     /locus_tag="VBE34_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033944.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            44089..45150
                     /locus_tag="VBE34_000430"
     CDS             44089..45150
                     /locus_tag="VBE34_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7263173.1"
                     /translation="MNKITTLSTIALTLMLCVGCSANKDNQKTKTEDPKQENVTANPK
                     EDKATAAKRMKLTKELEKYNKGTESFISNKQYQKYIKTPIKEYGQKVEVKGKKMNVYT
                     VGEGKVPIVFIPGQGTVTAKHQYHNLISNLSKTHKVVVVEPFGSGLSDVIDQPRNLAN
                     ITSDIHEALQKVGITGKYVIASHSIGGVYALKYISTYPKEVLGLIGLDTSTPGMEGGK
                     QVDFAAPVLKELPKIPKVSDDINAQFFAIGHKILNNSNMKEEAKNSSNMINESANYKI
                     PKGIPAMYLLARESDLGLQLRSLDIPIKGSWQEQHKELSEDPKEVSIHTLDGTHQIYD
                     TNLKEVIDYTNQFMKTFEK"
     gene            45274..46503
                     /locus_tag="VBE34_000431"
     CDS             45274..46503
                     /locus_tag="VBE34_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7263174.1"
                     /translation="MNLWTLSLAYLKRQKMKTVTLFLVFLTIGTCLISLMSIQHSLEK
                     NILTKQGKSIYLTSKEKAYWPEQAYEALKKAKMVESVEASLSIDVGSRLKSVSPYNSS
                     KEENQVTLAGYQSTEDLRAFQTKALVLKKGSHLAADNTKQVLVPLKLAQKNHLSVGNK
                     LRLGKENVTIAGIYDANSAKSKNTFNPNIDNTLIAQATLVRKISKQKGYQTVAVRLSD
                     KRLVDTVIQNIKQWPLDFGKLDVQTAKEFYGDSYRNIETLHRLVGRIILIVSLVAMAI
                     LVVMLTFWINNRIKETGILLAIGKTKFEIIGHYLIEVLLVAGAAFTLSIIGGVFLGKT
                     FAAGLLSQVNGGVSSQIVQNSSLIIDRIDNLAVSVGVMDVFRLYAQGALICLFAVVLS
                     SYSILKLQPKQILSRMS"
     gene            46513..47307
                     /locus_tag="VBE34_000432"
     CDS             46513..47307
                     /locus_tag="VBE34_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7263175.1"
                     /translation="MNLFKRSFLYVSRKKRKSITLFVCLWLVASTLISGIAVKNAGLT
                     AKKTFSRQTGSILHISSDSTDLVGDGYGSGEIPEKAIVNIASNPNVKRVNNNLMAYAG
                     LTSEKMVTRPNDKEQYKEQVLQVHGNSYSDTDPKYTAGMISLKGGRHITPKDQRVIIV
                     HQAFAKTNHLKVGDKLMFSKDPVRNSKDKTLVEATIVGIYKGKTVQKSNYPYEMLENT
                     AFSDTQLIKDLYGYSKGKAFYRQTTVYPKKNADIETLKSFIKKQSY"
     gene            47408..47536
                     /locus_tag="VBE34_000433"
     CDS             47408..47536
                     /locus_tag="VBE34_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000962057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO7263176.1"
                     /translation="MLVVSIILVMMALLFWIGGRTHETGILLAIGKSKAIIIVSTQ"
     gene            47545..47865
                     /locus_tag="VBE34_000434"
     CDS             47545..47865
                     /locus_tag="VBE34_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO7263177.1"
                     /translation="MIAIPAFALSVLISQFIGQKLADSLVYQASRTVRADFMKGFGNM
                     QLGADSDTDMFMQTIKTLNVQVKPEEVWLVILVGLAIVLFAIITTSLIIARYKPRGIL
                     SKLS"
     gene            47962..>48027
                     /locus_tag="VBE34_000435"
     CDS             47962..>48027
                     /locus_tag="VBE34_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7263178.1"
                     /translation="MLELKNIAYRYKGNDNKTLENI"
BASE COUNT        16648 a         7371 c         9251 g        14757 t
ORIGIN      
        1 ccctaaggaa gtcttctttt gaattgtaat gattatagaa agatcttcta ctaagtcgag
       61 ctttttttgc taaatcgcta actttaattt gctcaaagct cctcatcttt aacaattctg
      121 agaaagcttt taaaaatttt aaattagtgt tatctgaact taccaatata ctatcacctt
      181 tatataaaat cttaaagtta taaatcttat cgaatgtgat tatacaatac aaattcaaat
      241 aatagttcac atttatcgag ataatgtaaa acagtaaaac tttaaaaaaa gtaaaaatag
      301 ataactttat tggttaatct atttttactt ttgaaaagga gagaagttat gcttaggaag
      361 tataagcaaa gaaattataa tgtcgcacat acgaaacagc gtttttcgat taagaaattc
      421 aaatttggtg tagcatcggt attaattgga ttgacatttc taggaatgag tagtcattct
      481 gttttggcgg atgagtcaag cattatgagt actgaggtac cacaaacagc tggtgtaaaa
      541 ccggaagtta ctgttacaca gcctacttca gatgtaacaa gtcttgcgac agatacgtca
      601 acttcagcag tgttatcaac agatgcatct cattctaatc ctacagaatc agcggagcat
      661 gctgtaggat tagaatgctg aagatgcagc ggttccagtg acacacgaaa aagaagcaaa
      721 taaaacattg cgagttactt cttcttccac atctgaattc acagagtgga ctaatgtcaa
      781 ataagtagac agaaaaccgt gttattttat tgcgttaaaa taattttctt ctttctgatt
      841 aggggttagt cctagattag ccgtatgtgg gttgtaattg ttataaaaat tctcaatgta
      901 ttcaaagcag tctaattgaa cctgtttgat attttgataa tgttttcggt tgatttgtct
      961 atgctttaaa tacttgaaaa atgcttcagt tacggcatta tcataaggat atccaggttt
     1021 agaaaaagaa tgcatgatat tgttgtcatc aattattttt ctaaattccc tggccttaaa
     1081 ttgtgaccct tggtcggaat gaaagagaag tgttccttca atctttcttt tattaagagc
     1141 taattctaga gtgttacatg ctaactttgc atccatacgg tgactcaatt tccaagcaat
     1201 acattttcta gaatagagat caagtattgc gcagagatag acatatttct tatatccaat
     1261 agaaatatag gtgaagtcag tagaccatac ttgattaggt ttgtcaggat aaaatttctg
     1321 gttgagtaag ttttgaggat acgttttttg agtcttttta agagctgttt ttggcttaac
     1381 ggtagccatt ttagggagcg ccatattctt catcagatgg taaattcttc cttcagagat
     1441 ttttgtgtcg tagtctcttt gaagaatgac tttgatagac ttcacaccaa gtcttttgtt
     1501 tgctttggta taaatctcaa gtatttgttt tttataaatt tgattatcca attctctttt
     1561 tgagggctta tgttttagaa atttatagta agtggagcga ttgacacgta aaacacgaca
     1621 caaggttgtt gtggcgtgtt caaagcgtaa ccgatagaca gcgaggagtc ttactttaag
     1681 ttttgcatga atatggcact tgcttttttt aggataaggt tctcctcttc aagctgcgca
     1741 ttacgttttt gtaactcttg tatttgcttg gcagtaagta cagaattatc ttcgagtttg
     1801 aattgagagt actgtttgat ccattttgca agcgcagaag tggataccct atagtcttta
     1861 cagagttcag actgagtttt tccagtttga taaaggttaa caagagattg tttgaattct
     1921 tcatcatagc gtttaaatgt tgacataggt tgtcctttcg tttttgtgtc ttttaaaaca
     1981 gattgtaaca cacaaagttt tgtctacttt tatagtatac atccaccctt tggcaatacc
     2041 aaaagtttga gcagattagt tgagatgtgc aggaagtttt gattatcgca agagagaatc
     2101 atcaagtgac tcatgaacac gtgtcaatct tattgacctg cgttcaagaa ttgattgtgg
     2161 aagtcaatca aacacagcca ctcagtcgtg agtttcgtga aaagtatatg ggttaggagg
     2221 ctgcaattgt ttatcgttat cgtaccaatc tcaaaaaagt ctttctaaca gattcagaat
     2281 tgaatcaatt aaatgagcgg attgctaaga gcaattgtca aaatttctca gtctatgcta
     2341 gaagagttct tcttaatccc aatatgtcat ttgtcacgat taacacggat tggaaataac
     2401 atcaatcaaa ttgcgcgtgc tatcaaccaa agccatctaa tttctcaggc tcagtatcac
     2461 tttcatcgag aagcacgtta agcccaacag cagggggggt tgctctgatt cggttaagta
     2521 ataagcaaaa tcctcaccaa ttttcccagt aataagaggc gttgttgagg tatagggatt
     2581 acctgtgcca tagtcgatga tggtcacaaa atgaccattc cccataaaag agccaaccaa
     2641 aacttcacct gtggccgttt tttgatgtca acaccagtat tttggatgta ccccttaaca
     2701 tgccctttgg tatctgcaac agagataata tgaccaaagg aagaatcacc gatgacttta
     2761 accgtaatct tgctatcgcc tttttgatta gccgctaata tttgattagc aatcagggtg
     2821 cgaccaagag caactgttga agacgacaag gtatgatgtt tttcctgagc caatttaaca
     2881 gtctcggtgc tatcgaggac ataagacctg aaggcaccag aggcagaaat tgatttaata
     2941 attttatcca taacctatgt tatagcacaa agagagagtt tgtatggact ggattagctg
     3001 aaaattttag actaaaaagt agtgccagtg gattttgaaa ggtaacaaaa gagtcttccg
     3061 tttaaacgag aaggctgttt ttagtcgttt cagatgaagg caaaaggacg atgaaataat
     3121 tccgtacctt catggattgc tatgttactg gcatgaggtc tcacgatatt tagtaagata
     3181 ttcgtttgaa gatattccca cgtatttttt aaaggtttta agaaaatgtg tggtgtcgta
     3241 aaaatgtaat aatttcgcta cttccccaat cggtacccct ctttgcagta gtaattgtcc
     3301 ctccttaatt tttgccttta gaatataact ttgcaaggaa atgtcagagt attttttaaa
     3361 aactgagcgt aagtgggaat ctgagatata taggtagttg gcaatatcag atactttgag
     3421 tttggagtag aggtggttgt ccacataatg gagaatacta ttgtacattt gctgcttgtc
     3481 agagatgctc ttattggtta aggattctga aaaatcaata agagctgcac agcgaattct
     3541 tgaaacatca ataagatcag gagcctcttc gtttaaagcc atatagtgct ttaccagcgc
     3601 ataactttta gccacatcag tattttcctc gaaacttaat tctagtaatt ttgttaagta
     3661 aacaacagtt aagttctttt cagctcttag ggcagggatt gaagatgagg taacactgga
     3721 tgatgggagg cgattaattt cttgctttaa cagttgagtg ttacccagct taacgagatc
     3781 aataatgtga ttgagatggt ttaaaacagt gggtaactga aaagagtttt tcttagtatg
     3841 ttttaggtga agaacaatat caggatccgc aacaatctgt tctgactctt ctaataaatg
     3901 attgatgact tgttggcaac tagcctcaaa ctgtgtttgg aaaaaggcat cgatagacac
     3961 aagaagacta cgtatactgg tagtaggaaa acaagggaca agctttatat aggataagat
     4021 ttctttttta ctacgatgag aaaattgttc tagaaagcga ctggataact gttcttgcct
     4081 attgatatca gggctatagg gataaaatgg tccaatagca ataagatatt gacagacagg
     4141 aaaaattaag aatgattctt caaaaagacc ctcataaaca gtgatatctt ggttataagg
     4201 gatagctaaa tgttttaaaa actgatagta aggcaacaga tagtcttcgt tactatataa
     4261 ctgaacgagt tccttgtctc gtgacatgac tgaaataggt agttgagata tggtatgcaa
     4321 tgtttgaaca tgtttaaaat cgaatgtaac catttgatag accgccttca ttatcatttc
     4381 tagaattttt ctttaggttt gtaaagacta caaaataaaa tgatgaaaac aactatcttg
     4441 tggatacact aaaaagacac gctaattagc aaactctctc ttcatcatct ctcaccatta
     4501 ttatactact atttatatga caaataaagg tgattttgtt aaaatataac tttgaaaatc
     4561 cacatatatt tttaatcttc cgtctgaaaa aataaataaa aatagtaaaa ataaacacga
     4621 atttaaaata agcaaatttt ttaagaaaat ctgtgctaaa ctttaatagt tttgtgctta
     4681 ataataatca gcacttacaa agaacaaagg gaaaagcgag gagagaactt ttaatgaaat
     4741 tatcgaagaa gttattgttt tcggctgctg ttttaacaat ggtggcgggg tcaactgttg
     4801 aaccagtagc tcagtttgcg actggaatga gtattgtaag agctgcagaa gtgtcacaag
     4861 aacgcccagc gaaaacaaca gtaaatatct ataaattaca agctgatagt tataaatcgg
     4921 aaattacttc taatggtggt atcgagaata aagacggcga agtaatatct aactatgcta
     4981 aacttggtga caatgtaaaa ggtttgcaag gtgtacagtt taaacgttat aaagtcaaga
     5041 cggatatttc tgttgatgaa ttgaaaaaat tgacaacagt tgaagcagca gatgcaaaag
     5101 ttggaacgat tcttgaagaa ggtgtcagtc tacctcaaaa aactaatgct caaggtttgg
     5161 tcgtcgatgc tctggattca aaaagtaatg tgagatactt gtatgtagaa gatttaaaga
     5221 attcaccttc aaacattacc aaagcttatg ctgtaccgtt tgtgttggaa ttaccagttg
     5281 ctaactctac aggtacaggt ttcctttctg aaattaatat ttaccctaaa aacgttgtaa
     5341 ctgatgaacc aaaaacagat aaagatgtta aaaaattagg tcaggacgat gcaggttata
     5401 cgattggtga agaattcaaa tggttcttga aatctacaat ccctgccaat ttaggtgact
     5461 atgaaaaatt tgaaattact gataaatttg cagatggctt gacttataaa tctgttggaa
     5521 aaatcaagat tggttcgaaa acactgaata gagatgagca ctacactatt gatgaaccaa
     5581 cagttgataa ccaaaataca ttaaaaatta cgtttaaacc agagaaattt aaagaaattg
     5641 ctgagctact taaaggaatg acccttgtta aaaatcaaga tgctcttgat aaagctactg
     5701 caaatacaga tgatgcggca tttttggaaa ttccagttgc atcaactatt aatgaaaaag
     5761 cagttttagg aaaagcaatt gaaaatactt ttgaacttca atatgaccat actcctgata
     5821 aagctgacaa tccaaaacca tctaatcctc caagaaaacc agaagttcat actggtggga
     5881 aacgatttgt aaagaaagac tcaacagaaa cacaaacact aggtggtgct gagtttgatt
     5941 tgttggcttc tgatgggaca gcagtaaaat ggacagatgc tcttattaaa gcgaatacta
     6001 ataaaaacta tattgctgga gaagctgtta ctgggcaacc aatcaaattg aaatcacata
     6061 cagacggtac gtttgagatt aaaggtttgg cttatgcagt tgatgcgaat gcagagggta
     6121 cagcagtaac ttacaaatta aaagaaacaa aagcaccaga aggttatgta atccctgata
     6181 aagaaatcga gtttacagta tcacaaacat cttataatac aaaaccaact gacatcacgg
     6241 ttgatagtgc tgatgcaaca cctgatacaa ttaaaaacaa caaacgtcct tcaatcccta
     6301 atactggtgg tattggtacg gctatctttg tcgctatcgg tgctgcggtg atggcttttg
     6361 ctgttaaggg gatgaagcgt cgtacaaaag ataactaaat aaaaggctac ttcttaagta
     6421 accatgttta agaaaagaga aatagcctta tttctctttt tgtcgttttt aaaataaagg
     6481 aacatcatga aacaaacatt aaaacttatg ttttcttttc tgttgatgtt agggactatg
     6541 tttggaatta gccaaactgt tttagcgcaa gaaactcatc agttgacgat tgttcatctt
     6601 gaagcaaggg atattgatcg tccaaatcca cagttggaga ttgcccctaa agaagggact
     6661 ccaattgaag gagtactcta tcagttgtac caattaaaat caactgaaga tggcgatttg
     6721 ttggcacatt ggaattccct aactatcaca gaattgaaaa aacaggcgca gcaggttttt
     6781 gaagccacta ctaatcaaca aggaaaggct acatttaacc aactaccaga tggaatttat
     6841 tatggtctgg cggttaaagc cggtgaaaaa aatcgtaatg tctcagcttt cttggttgac
     6901 ttgtctgagg ataaagtgat ttatcctaaa atcatctggt ccacaggtga gttggacttg
     6961 cttaaagttg gtgtggatgg tgataccaaa aaaccactag caggcgttgt ctttgaactt
     7021 tatgaaaaga atggtaggac tcctattcgt gtgaaaaatg gggtgcattc tcaagatatt
     7081 gacgctgcaa aacatttaga aacagattca tcagggcata tcagaatttc cgggctcatc
     7141 catggggact atgtcttaaa agaaatcgag acacagtcag gatatcagat cggacaggca
     7201 gagactgctg tgactattga aaaatcaaaa acagtaacag taacgattga aaataaaaaa
     7261 gttccgacac ctaaagtgcc atctcgagga ggtcttattc ccaaaacagg tgagcaacag
     7321 gcaatggcac ttgtaattat tggtggtatt ttaattgctt tagccttacg attactatca
     7381 aaacatcgga aacatcaaaa taaggattag catgggacaa aaatcaaaaa tatctctagc
     7441 tacgaatatt cgtatatgga tttttcgttt aattttctta gcgggtttcc ttgttttggc
     7501 atttcccatc gttagtcagg tcatgtactt tcaagcctct cacgccaata ttaatgcttt
     7561 taaagaagct gttaccaaga ttgaccgggt ggagattaat cggcgtttag aacttgctta
     7621 tgcttataac gccagtatag caggtgccaa aactaatggc gaatatccag cgcttaaaga
     7681 cccctactct gctgaacaaa agcaggcagg ggtcgttgag tacgcccgca tgcttgaagt
     7741 caaagaacaa ataggtcatg tgattattcc aagaattaat caggatatcc ctatttacgc
     7801 tggctctgct gaagaaaatc ttcagagggg cgttggacat ttagagggga ccagtcttcc
     7861 agtcggtggt gagtcaactc atgccgttct aactgcccat cgagggctac caacggccaa
     7921 gctatttacc aatttagaca aggtaacagt aggtgaccgt ttttacattg aacacatcgg
     7981 cggaaagatt gcttatcagg tagaccaaat caaagttatc gcccctgatc agttagagga
     8041 tttgtacgtg attcaaggag aagatcacgt caccctatta acttgcacac cttatatgat
     8101 aaatagtcat cgcctcctcg ttcgaggcaa gcgaattcct tatgtggaaa aaacagtgca
     8161 gaaagattca aagaccttca ggcaacaaca atacctaacc tatgctatgt gggtagtcgt
     8221 tggacttatc ttgctgtcgc ttctcatttg gtttaaaaag acgaaacaga aaaagcggag
     8281 aaagaatgaa aaagcggcta gtcaaaatag tcacaataat tcgaaataat aaaatcagaa
     8341 ccctcatttt tgtgatggga agtctgattc tcttatttcc gattgtgagc caggtaagtt
     8401 actaccttgc ttcgcatcaa aatattaatc aatttaagcg ggaagtcgct aagattgata
     8461 ctaatacggt tgaacgacgc atcgctttag ctaatgctta caatgagacg ttatcaagga
     8521 atcccttgct tatagaccct tttaccagta agcaaaaaga aggtttgaga gagtatgctc
     8581 gtatgcttga agttcatgag caaataggtc atgtggcaat cccaagtatt ggggttgata
     8641 ttccaattta tgctggaaca tccgaaactg tgcttcagaa aggtagtggg catttggagg
     8701 gaaccagtct tccagtggga ggtttgtcaa cccattcagt actaactgcc caccgtggct
     8761 tgccaacagc taggctattt accgacttaa ataaagttaa aaaaggccag attttctatg
     8821 tgacgaacat caaggaaaca cttgcctaca aagtcgtgtc tatcaaagtt gtggatccaa
     8881 cagctttaag tgaggttaag attgtcaatg gtaaggatta tataaccttg ctgacttgca
     8941 caccttacat gatcaatagt catcgtctct tggtaaaagg agagcgtatt ccttatgatt
     9001 ctaccgaggc ggaaaagcac aaagaacaaa ccgtacaaga ttatcgtttg tcactagtgt
     9061 tgaagatact actagtatta ttaattggac tcttcatcgt gataatgatg agaagatgga
     9121 tgcaacatcg tcaataacga tgttgtgaat ggcttactta cttatcaaat aggtgactaa
     9181 tgatgattgt gaataatggt tatctagaag ggagaaaaat gaaaaagaga caaaaaatat
     9241 ggagagggtt atcagttact ttactaatcc tgtcccaaat tccatttggt atattggtac
     9301 aaggtgaaac ccaagatacc aatcaagcac ttggaaaagt aattgttaaa aaaacgggag
     9361 acaatgctac accattaggc aaagcgactt ttgtgttaaa aaatgacaat gataagtcag
     9421 aaacaagtca cgaaacggta gagggttctg gagaagcaac ctttgaaaac ataaaacctg
     9481 gagactacac attaagagaa gaaacagcac caattggtta taaaaaaact gataaaacct
     9541 ggaaagttaa agttgcagat aacggagcaa caataatcga gggtatggat gcagataaag
     9601 cagagaaacg aaaagaagtt ttgaatgccc aatatccaaa atcagctatt tatgaggata
     9661 caaaagaaaa ttacccatta gttaatgtag agggttccaa agttggtgaa caatacaaag
     9721 cattgaatcc aataaatgga aaagatggtc gaagagagat tgctgaaggt tggttatcaa
     9781 aaaaaattac aggggtcaat gatctcgata agaataaata taaaattgaa ttaactgttg
     9841 agggtaaaac cactgttgaa acgaaagaac ttaatcaacc actagatgtc gttgtgctat
     9901 tagataattc aaatagtatg aataatgaaa gagccaataa ttctcaaaga gcattaaaag
     9961 ctggggaagc agttgaaaag ctgattgata aaattacatc aaataaagac aatagagtag
    10021 ctcttgtgac atatgcctca accatttttg atggtactga agcgaccgta tcaaagggag
    10081 ttgccgatca aaatggtaaa gcgctgaatg atagtgtatc atgggattat cataaaacta
    10141 cttttacagc aactacacat aattacagtt atttaaattt aacaaatgat gctaacgaag
    10201 ttaatattct aaagtcaaga attccaaagg aagcggagca tataaatggg gatcgcacgc
    10261 tctatcaatt tggtgcgaca tttactcaaa aagctctaat gaaagcaaat gaaattttag
    10321 agacacaaag ttctaatgct agaaaaaaac ttatttttca cgtaactgat ggtgtcccta
    10381 cgatgtctta tgccataaat tttaatcctt atatatcaac atcttaccaa aaccagttta
    10441 attctttttt aaataaaata ccagatagaa gtggtattct ccaagaggat tttataatca
    10501 atggtgatga ttatcaaata gtaaaaggag atggagagag ttttaaactg ttttcggata
    10561 gaaaagttcc tgttactgga ggaacgacac aagcagctta tcgagtaccg caaaatcaac
    10621 tctctgtaat gagtaatgag ggatatgcaa ttaatagtgg atatatttat ctctattgga
    10681 gagattacaa ctgggtctat ccatttgatc ctaagacaaa gaaagtttct gcaacgaaac
    10741 aaatcaaaac tcatggtgag ccaacaacat tatactttaa tggaaatata agacctaaag
    10801 gttatgacat ttttactgtt gggattggtg taaacggaga tcctggtgca actcctcttg
    10861 aagctgagaa atttatgcaa tcaatatcaa gtaaaacaga aaattatact aatgttgatg
    10921 atacaaataa aatttatgat gagctaaata aatactttaa aacaattgtt gaggaaaaac
    10981 attctattgt tgatggaaat gtgactgatc ctatgggaga gatgattgaa ttccaattaa
    11041 aaaatggtca aagttttaca catgatgatt acgttttggt tggaaatgat ggcagtcaat
    11101 taaaaaatgg tgtggctctt ggtggaccaa acagtgatgg gggaatttta aaagatgtta
    11161 cagtgactta tgataagaca tctcaaacca tcaaaatcaa tcatttgaac ttaggaagtg
    11221 gacaaaaagt agttcttacc tatgatgtac gtttaaaaga taactatata agtaacaaat
    11281 tttacaatac aaataatcgt acaacgctaa gtccgaagag tgaaaaagaa ccaaatacta
    11341 ttcgtgattt cccaattccc aaaattcgtg atgttcgtga gtttccggta ctaaccatca
    11401 gtaatcagaa gaaaatgggt gaggttgaat ttattaaagt taataaagac aaacattcag
    11461 aatcgctttt gggagctaag tttcaacttc agatagaaaa agatttttct gggtataagc
    11521 aatttgttcc agagggaagt gatgttacaa caaagaatga tggtaaaatt tattttaaag
    11581 cacttcaaga tggtaactat aaattatatg aaatttcaag tccagatggc tatatagagg
    11641 ttaaaacgaa acctgttgtg acatttacaa ttcaaaatgg agaagttacg aacctgaaag
    11701 cagatccaaa tgctaataaa aatcaaatcg ggtatcttga aggaaatggt aaacatctta
    11761 ttaccaacac tcccaaacgc ccaccaggtg tttttcctaa aacaggggga attggtacaa
    11821 ttgtctatat attagttggt tctactttta tgatacttac catttgttct ttccgtcgta
    11881 aacaattgta aggtgtcgtt gaaattatta aataatagaa aatgactagt tttggccttt
    11941 ccctattgtc agtcagatta gttattatca agcttctcat gccaatatta atgcctttaa
    12001 aagaacagtt acaaccattg accgtacgga gattcaacga cggttaggtc tggctaaagc
    12061 ctacaatgct agtatttctg gaacaagtag tcagtcgact caatctgtgc tgagagattc
    12121 ttattctgag gagcaaaaaa ggcagggtgg actgaatacg ctaggatgtt agaagtcaga
    12181 gagcaggttg accatgtgat gattccaaaa atcaatcagg atttaccaat ctacgctggt
    12241 tcagaagagg acaatctgca acggggagtt ggtcatctag aagggataag tttgccgatt
    12301 ggaggggctt ctacacatgc ggtcttgagc ggtcaaagag gtatgccagc tgctcggttg
    12361 tttgcggatt tggataagat gaaaaaaggt gattattttt atgttaccaa tctgaaagaa
    12421 accttggctt atcaagtgga tcgtatcatg gtgattgaac ctagccaatt ggatgccgtg
    12481 agcattgaag aggataaaga ttatgttacc cttctgacct gtacacctta tatgggctct
    12541 ttgtcaactg taatgggtga cttatcatta acaacgagag agaatcagct tggttctctc
    12601 tctttttgga tgttcaaagc gatgagaatt ttgcttttaa aatttttaaa gttgcgaaag
    12661 ccaaaggctt gtcgcttgat gtctttgata agcttgttgg tcgcttctaa tttggcattg
    12721 gaaagggata gctgagggcg tttgtgatgt aagttttgta cttcatgaag gtcctaaaga
    12781 ccattttaaa agcagaattg ccagttgtta cgttatcatc tatgaagcta aagaattcag
    12841 ttgttcgctt ttcttagaaa tgaaagagta tgagctgata aaggtcgtta taatagcgga
    12901 gctcatctga gaaaactttt atacctcaaa gtcagtctag ctttgatatc atgaagccat
    12961 taggagttat tccttatctt ttagtggcgc gcgatccata tagtgataga tcgagatatt
    13021 tagatccaaa agttctatca tcctcttttg gcgccttttt tccagcagat aatattaagg
    13081 tagcttggtc taacaactcc agcactttat ttacaccacc tattaatgca aactacacca
    13141 ctcagattca agctattggg acaacgatta agtcacaaat tccggaatcg attttgacgg
    13201 ttacggataa aaagagcagg aagttcagca ttaacaagat tgacgaagct aaagaaggct
    13261 tagtaggtgc gaccttcacc ttgtctaaac gcacaacagt agcggcagat catcaagtac
    13321 aaggagattt cattcctgtc agcaaagaga cgacagtcgg tcggacaacc cttacctttg
    13381 acaaccttaa acctggattt tatgacctta aagaaacgaa agcgccgaat gcttacgtac
    13441 ttgatcctaa gacttatgtt gtggtcgttc aaaattcagg aaaaacgaca attgtggatg
    13501 aagctaactt caaagaggct gattacccaa tggctgataa taccagccat gtggagtgcg
    13561 tagcgttgct acaacgaagc aaagggtaaa aatcctttat tttaagcact ttttcaagca
    13621 ttttgtcttt attgaaaaga gtgattttaa cataaaaaag gtattaaaaa acatattgac
    13681 gtgaccgttt gttttgaagt ggcttgcgta gacaaaaaaa tagatacgtc agataaattt
    13741 ctggcattac gagaacattt ttagagtgtt ctcttttttt agtttacgga ggaaaaatat
    13801 atatggaaaa acaggattca cgagttctca tccattggga ggggaattct ggggacaagc
    13861 tcattgaaca ccaaaccagc gcaacggggt ggtactacca agtcgatcgt agctttagtc
    13921 aaccaaaagg ggaaccgccc agaatgatcc aaaggcacta gaaagtgtcc gtaatgattc
    13981 gatttcgggc ggtgatgatg tcatgggtta tgcttatagc aaatgtactt ggggagttgc
    14041 ggcacgaatt aatcagtggg actgaaactc aaaggttgaa atggtgagaa gattaccatt
    14101 accagttcaa tgggaaatgg tcaggattgg gttggaacag ccgaaagact agatggtgaa
    14161 actgatacag ttccaaaaga aggtactatt ctctcttttt aggaaagtag ttatggttcg
    14221 tatataggct acggaactat atctttcgtc acattacatc tacagatagt accatgaatt
    14281 ttgcttatat gaccaagtaa agtgaggata tactaacaaa tgaaatattt attatcgtat
    14341 ttgtccattt tatcgaaaag tttgcatatt atcattatgt ttgataagat gcaaatataa
    14401 tgatagtagg agctaaatat ggatatttaa aaaatcaaga gtatcctaag tgctttccat
    14461 tttgaaattc aaatatagct aatagttcta gaacttctaa ttgtttttcg tcgacgatat
    14521 gaattttcaa tcttaactgt taggattcca cctccctttg gttaaagaaa aaaggtcagg
    14581 tcgtttagat aactttgtca aacaagctca agctatctaa aaatagtttg aaatgggcat
    14641 tactctagtt tttaataagc tatctgatga gcagaaggag aagttaatgc atgttgggaa
    14701 gtcttatttt gactatcaag aaaatgctct tatcccacaa ttaggttttc tatattctaa
    14761 attaactaaa aaaattgaac ttgataatcg gttgtctccg actgaacaaa agttattgat
    14821 taccttatta ttacatacta aaggtttaat cattgatatg taagaagtaa gtcagctaac
    14881 cgatctttct attctaaaac ttatattgtt gctttagaaa ttttaaagag cgtggatggc
    14941 ttcataataa acagaaatct taccaatttg cgaagccaaa aaatatgata tttgaagagt
    15001 ccaaagatct aatagatagt ccagttagag aagcgttgat tataagtgat aaggattttc
    15061 aaaaattaaa acaagagcta ttattttaac cgacttattt taaagactta tcatatctag
    15121 gcttgcttga tgattcggaa aaatacggag actatactat ttcaaggaaa agatacaaaa
    15181 gtttcgaatc aagtcttcaa ctatacatcc ttcaaagtca tcggctagag atttggaatt
    15241 atgaaccaat ccctttgatt actagaaaaa taaatagctt ggagaggcta acgtgacact
    15301 ggttgatcca atctcgcttt atttaacact aagaatgatg aagaccctcg tattgaagaa
    15361 gaagttgagc agctagaaga taagattttg agaatataat cgttttttcg tgctacacta
    15421 acttgtcagg gttgcaacag acaaaaaaat aatttaggag ggattcgtta tgcgtacaaa
    15481 gtatatagga tatgaaattg ttttaagggg tggttattaa tatggataag aaaaaggttg
    15541 tttcaactat cgtatctaca gggtttctta tagtttcaat atcaaatttt tttagagagc
    15601 tatcacctat actttttctc ttgaatatta ttggattttt ctgcttccta ttattatttt
    15661 ctgccaacat aaataaattg ttcttaaaac gatagccgtt atataggata tggtatgtat
    15721 gcatttggtg aaattatagg taaaacgaat ggaatagtac ttactcctca aagctatcgt
    15781 gagagatata aaaggatatc gaggagtcac tagatagttg gatctcattg aaaaaatttg
    15841 tttaaagtat ctcatataag cataaaacga ggaatcaatc aaaatttctt gattgattcc
    15901 ttgttttttg gctctttgtc aactgtagtg ggttgatgaa aaattatatt ctggagaaaa
    15961 ccaaattggt tttctccttt ttgatattca aagtgatatg aattctcttt ttaaaatatt
    16021 caaagtttct aaagctaaat gtttgtgctt gatgattttg atgagattat tggttgctca
    16081 agtttggtgt ttgaataagg cgattcaagg acattgataa ccttatctct gtctttaaaa
    16141 aatgtcctaa aaacagttta aaagattgga gtgacactag agaaagtgtt ttaaacaggg
    16201 taaagaaatg gtgagcttgc ttctcttgaa agtaaagcag gaggagttga tagagtttca
    16261 taatgttttt tatagagaag cgatatcttc ttatcttgaa acagaaaatt ttgagaaggc
    16321 taaagtgact gaagagaatt accaagtttt tttaggaatc cgtacttatc agaagagtta
    16381 taactatcaa gatcatagtg agaatctaaa aggaaatatt gaggtagcaa tgataggagt
    16441 tgaaaataat attagcgacg aacatatttt ttgctagaat atataaaatg tctttgaaat
    16501 aatttagccc tgtgttatga tattaaggag gttatttatg gtacgtaaat ttttaaaatc
    16561 gctcctatct ctctttctta tagcagtaat cgccaccggt atttcagtgg catgtttctt
    16621 ttttattcca gaaaataaag ggaatattac accgatttta ttgcatcgct ttatgcgaaa
    16681 aaataatgtt aatggaatga tgattgttag cgataatact ggtaaaccga ttaccatctc
    16741 gcatgggatt aaccgtgggg aagttgaaac ggatattgaa aataataaat tatttccaat
    16801 ggcttctctc caaaaactca tgacaggaat tattatccaa agattaattg atcaagatgt
    16861 cttgagtgaa gacgaccgat tgtctcagtt ctttccccaa gtaaaaggta gtaatagtat
    16921 taccatccac caattgttga cccatacgag tgggttaaga gaaaaaggtg ttaaggtgtc
    16981 tccttatcta aaaaatgaga gagaacaatt acaattttgc ttaaaacact ataactttgt
    17041 taataagaaa tcgtggtatt attctaatat caactttagt ttcctaacag gtatagccac
    17101 tcaagttacg ggaaggacct acgctgaact tgttgatgat gtgataaaaa atcctttgcg
    17161 attagatgat acccaaagtt accaatcagt tgttaatcat gacttagtta gcccaatgcg
    17221 aaagaacggc aagttaaata aaattaatat ctttaatcaa gtatccacag catatggagc
    17281 tggagatttt ttcacaactc cgctgaactt ttgggtgttg atgcggtctt tttctaaagg
    17341 gtactttttc ccaacggatg aatataccaa acatcaaaat gatgcgatat cacattatta
    17401 tggtgggctt tacatgcatg gtcgtatagt taattctaat ggggcctttt tccagtattc
    17461 aagttttggt tatgcagatc taaagacgca tgatacaatg gtgcttttta tgaataacaa
    17521 agtctatagt gatgctagtc acgtggcacc aaaagcattt gaatattatc aaaaatttat
    17581 gttccttaat aagattttcc acttagtctt ttactttgta ttttctttct ttttaatgct
    17641 tttaatacgt cattcgttta ggaaaagaag gtataagaaa cgtctctaaa tcacatgaat
    17701 aaaaaagaag gctaagttgg ccttcttttt tattcaatat taagcagtaa caccatctaa
    17761 gaaaacgttt gcattgaatt tttaattgtg ctataataaa tagctgttaa tcattattat
    17821 agtttaataa tctaactgtc gagctgattt aagctataaa aatactgttg agtaaaagag
    17881 gtattaatat gtcaatgatt gaagtttctc accttcagaa aaattttata aaaaccgtta
    17941 aagcaccagg gttaaaagga gcatttcaat catttcttcg tcctgaaaaa cacacctttg
    18001 aagctgttaa agatttaaca tttgatgtgc ctaaaggaca aatttgggga tttattggag
    18061 cgaacggtgc aggaaaatct acaacaatta aaatgttaac tgggatttta aaaccaactt
    18121 cgggcttttg ccgtattgat ggcaaattgc cacaagaaaa ccgacaaaat tatgttaagg
    18181 atattggagt cgtttttggg caacgtacgt agttgtggtg ggatttagct ctacaagaaa
    18241 cctatacagt gttaaaagaa atctatgacg ttcctgataa agaatttcgc aaacgcatgg
    18301 cttttttaaa tgaggttttg gaactaaatg actttataaa ggatcctgtt agaactttgt
    18361 ctctcggaca gcgtatgaga gcggatatcg cagcttcttt attacataac ccaaaggttc
    18421 ttttcttaga tgaacccacc attggcttgg acgtttcagt gaaagataat atccgacgtg
    18481 caatcacaca aattaatcaa gaagaagaga caacgatttt actaacgaca catgacctca
    18541 gtgatattga acaactttgt catcgtattt ttatgattga tagaggacaa gaaatatttg
    18601 atggcacagt ttctcagcta aaagagacat ttggaaagat gaaaactctt tcttttgatt
    18661 tgcgcccagg acaagagcat atcagctcta gtttaattgg aaagtcggag attaatatta
    18721 aacgtaatga cttagtttta gatattcagt atgatagttc tcgctaccaa actgctgaca
    18781 taattcagca gacattagca gatttttctg tacgtgacct taaaatgaca gatgcggata
    18841 ttgaagatat cattcgtcgc ttctatagaa atgagctata aggatgaaca gaatttggag
    18901 acgttataaa ccttttatta gtacaggaat ccaaggatta atcacatatc gtgttgattt
    18961 catcctttat aggataggtg atgtgatagg agcatttgtt gcattttatc tatggaaagc
    19021 agttttcgat tcctcaagtc aatccttaat ccaaggtttt cagctgtcag atatgattct
    19081 ctatattatt atgagttttg tgactaattt attaacaaga actgatagtt catttatgat
    19141 aggtgacgaa gtaaaagacg gttcaattat tatgcgactc ttacgtccag ttcactttgc
    19201 tgcttcctac cttttcatgg agattggttc tagatggctc atctttttaa gtataggggt
    19261 accgtttttg cttgttatta cgggggtacg cctcttcttg gggactgatt tgattcaagc
    19321 tatagtgtta gtggtatttt atattattag cattatttta gcttttttga ttaacttctt
    19381 ttttaatatt tgttttggct tctcggcatt tgtttttaaa aatttatggg ggtcaaacct
    19441 gttaaaaaat tcgttagtcg catttatgtc ggggagcttg attcctctta ctttttttcc
    19501 gaaaatcgtt gctgatattt taggattttt accattttca tctttaattt acacaccagt
    19561 aatgattatt attggtaaat atgatggcag tcaaattgtg caagccttac ttttacaaat
    19621 attttggcta atagtaatgg tagctttatc tcaattaata tggaaaaaag tacagtcgca
    19681 tatcactatc caaggaggtt gagatgacaa aatatcagcg aatgcacttt atttttatta
    19741 aacaatacat gaagcaaatc atggagtata aaatagactt ttttgtaggc gttttaggag
    19801 tatttttaac acaagggcta aatcttttat ttctgaatgt tctttttcaa catatccctt
    19861 ctttagaagg atggactttt cagcaaatag cttttattta tggtttttct ctcctcccaa
    19921 aaggaattga tcaccttttc tttgataact tgtgggctct tggtcaaagg cttattcgaa
    19981 aaggagaatt tgataagtat ttaaccaggc cgatcagtcc tttatttcat gtcctagttg
    20041 aaacctttca agtagatgca ttgggggagt tattggttgg ttttattctg ttatctacca
    20101 cagtatctag tataagctgg atcgtgccaa aagtattatt atttatattt attatcccct
    20161 ttgcaacttt gatttatacc tccttgaaga ttgctacgtc aagtatcgct ttttggacca
    20221 aacaatctgg tgctgttatc tatatttttt atatgtttaa tgactttgct aagtatcctg
    20281 tcgcaatata taataatcta ttgcgttgga tcattagttt tgtcattcca tttgctttta
    20341 cagcttatta tcccgctgct tacttcttac aagatagaaa tgtctatttt aatattggtg
    20401 gggttatctt gatttccttg atttctttta tggtatcact aatactttgg cataaaggag
    20461 tagaagtata tgagagtgcg ggttcttgat tagtaaggca agccattaat ttttttgagt
    20521 gtttagaagt tttaggctta ctaacttagc tttgttagtg atgatatact ccccttatag
    20581 tgtctagtga atttttgttt ttttactgtc cactataagg ggagtatatc aggtgaagag
    20641 ggactattag tgttgagaat aatgtccctc atagaacaat aattaccata tctttaccaa
    20701 aataaagtta aaaacgctct tagatgtgct ttctaagagc gttttttggt ctataaaagg
    20761 taagcaaaaa gcagatgtca tttatgaaag tgttgacaaa gatgaaatac tttgttacta
    20821 ttttaacata ataatgatat tttaattaga gtgtgaattt ataattaatt tttacaatgt
    20881 ttattttaaa tttaaactaa tgtttatagc agtgttttac tcatgatttt gtgaattcaa
    20941 cacttaagtc atgacctcat tttaaagagt ttcagacgac acagaaagga agaaaacata
    21001 agaaaggcaa tgcacggctt gtccatagta gtgtttgaag tagatgttta gaaatatttt
    21061 attttccaac agtactgata tcagagcgaa gatgattagt tgaaggtgat aatatgggac
    21121 gttctaatat tttgagtagg tgtgaacaat gttctgataa tcaatgtggc ttgtataaaa
    21181 cgcggcttaa agacaagtta ctattagtag tccaactggt tggaaatatt aggtattctg
    21241 agtttcttac ggaaggtggt gtatatcata agggagaaac cttgttggaa gttataacct
    21301 taggaatttc ttatgaatat aaaagttatg gggatatcaa agtaatcaag aagtactgtg
    21361 ctgatggcag tgtcgttgat tataacatgc caatactatt aatggaaagg attgaataat
    21421 gaaaacagta ataatatatg gaggacaggg taatgttcag tcaaaacacc tttgtcaact
    21481 agttaaaact ccaaaaggtg aaattctttt ttcaaagtta caagcaactg atttagaatc
    21541 atatcagtta atgatggatg tcattaacca caaggtaagt ttagacaatg ttcatgcgtc
    21601 tatgttagca aattttttat ataatgattg gctaacaaac gatgaaaaaa tagtatctgg
    21661 gataggattt tcagctcata gcgccggtat tttcaatgtg ttatatgcta gtggtagtac
    21721 tagttttgaa aacattattc actttattaa acgtcgtgcc caacttatag aaaattgtca
    21781 tgtgtctgaa gagctttggc ttttgatggt tgaaaacatt gatatgttta gtaaggatgt
    21841 tttaaaagaa cagtctgaga aagtaaaatt agctattatt actgatgata gagctggtgt
    21901 aattgctatg gatcaagtca accgttcttt ctttgatgac gtgatgacta acaaaggtta
    21961 tttttataaa ttaaagcagt taggcgtaaa agcaccatat catactgatt ttttaaatga
    22021 atctaaagag cagtataaac aattgattga aagcctagat atttcgcaaa atgatgacta
    22081 tgactatatt tttcattgtg atcaattggt agatgaaatt ttatatcagt ggaccaatct
    22141 ctttaactgg cgtgcgatta aggaaactat catagaaaag gatgcggaga ttattgatat
    22201 ttctcctaat aggtttattt ctagtcagtt gcaaaaaatg aaaaagaggg ggaggaaaca
    22261 gttatgaaag tagcaatagt tactggtggt accagaggaa ttggaagggc tattacaaaa
    22321 gagttataca aagaaggtta caaagtcatt gctatttata atagcaatga tgctaaggca
    22381 agagctttac aagaggagtt accaaaatta gacgtttata aatgcaatat ttcagatgca
    22441 aaagcagtac aaaaattggt gactaaaatt tttagagaat atggcggtat tgattgttta
    22501 gttaataatg ctggtattgt tagagatggc tttttcttga tgatgtctaa ggaaaaatgg
    22561 atggatgtca ttaacataaa tattatgggg ctagttaaca tgagtaaagc cgtgttaaaa
    22621 atcatgaagg ctaagcgtat acagggaaag gttatcaata tttcttcaac tagtggtata
    22681 gcagggcaga tagggcaagc gaactattca gcaacgaaag gcgctatcat ttccattact
    22741 aaaactctag cgaaagaatt tgcatcagat ggtattacga ttaattgtgt aagtccagga
    22801 ttcattgaaa cagatatgac aaatgaattg caaaataaag aagagctaaa agagcatctt
    22861 attcctctaa aaaggtttgg acaacctgaa gaggtggctt ggctcgtaag ctttttagca
    22921 agtgagaagg caaactatat tacaggaaaa aatattgtga ttgatggagg aatgattaat
    22981 gattaactgg gctgaagaac gtaaaataat aaaaacacgt caagaagtag ttgatgaaat
    23041 taaaggtatt ttgattgact ctttaatgtt ggatattgat aaagatctga ttatgaatga
    23101 tcaaccatta tttggtcgtg gattagagct agactctatt gatgccctag aactgtcaat
    23161 agggatttca acaacgtttg gtgttgaact aaatgatgat gatatttcaa tattatcatc
    23221 agtaaataga ctagcagatt ttgtgattga aaatagtgag gatatagatg accaagctaa
    23281 gtagtgaaga aattataaaa atgttacctc atcgctatcc gtttctaatg gtggatagag
    23341 tgcagagtta taaaaaaggt atcattgaat gtcgtaagaa tattactatc aacgaacctt
    23401 actttcaagg acattttcca caacgtccta tagttccggg tgttttgatg attgagatgg
    23461 cagctcaatc tgcagcttta ctttatattt tagatgcact tgatggtagc ctcccctctc
    23521 ttgatgaatt aacagcagag aaaatagcag aaaaagtagg ttatttagcc agtgtgaaga
    23581 atttcaaatt caagaaaatt gttactccag gagaccagct aaacattaca tgtaaatctc
    23641 aatcaaaatt ggggcatctt ttagaaattc aagtcgttat tagagatgag aataaaaaag
    23701 aggttgcctc aggaaggatg ttggtatcag aaaatggaaa ttaaactcaa aaatattgga
    23761 aaaaaatatg gttcttttga agcattgaga gacattaatc tcatctttga agagggaaaa
    23821 ttttatggcc ttcttggacc taatggagct ggtaaaacaa ctttatttaa ccttttaatt
    23881 caaaatttta agcaaacgtc aggcgatatt aagtgggagg tggatggaaa acctctatct
    23941 attaaagatt tttatcgtca cattggtatt gtcttccaaa gtaatcgctt agatgataat
    24001 ttaacggttg aggaaaatct aatttctaga ggtgctttgt acggtttatc taaatcacaa
    24061 gtacgtaatc gccttaaaga tcttcaaacg tatctagata tcacagcaat aaaaaaacaa
    24121 aaatatggca gtttatcagg aggtcaaaaa cgaaaagttg atattgcacg cgccttgctg
    24181 ccacaacctt ctctattatt attagatgaa ccaacgactg gtttagaccc tcaatctcgt
    24241 cgcgatttat gggatgctat cgcacaacta aatcaacaat cgcagatgac agttgtacta
    24301 attactcatt atcttgaaga aatgtctgct tgtgatgttt taaatgttct tattgaagga
    24361 aacatttatt attcgggaga tattaaaagc tttattgagc aacattccac aacaaacttg
    24421 aatgtggtat tgaaaccaga aaagtcatta gaccaacttt cgatagctga ttttgttaat
    24481 aaatgtcaag tcctttcaga aagggagata gtatttaagg atatttctgt tgaggaaatg
    24541 atgcaggtta tctcagacaa tcaagataaa tctattattg agacatttaa tgttgaatat
    24601 tcgaacttag aggcggcgta tcttaacttg cttagagaaa aagaaaggaa aaacaatgtt
    24661 tgaactaatt tctagaaatc gtaaagttta cacgcgagat cggttagcgt ttttcatgtc
    24721 ttttttatca gttattatct taattttggt ctatcagatt ttcttgggaa aaattcaatt
    24781 agatgctata aaagcagcat tgaggagtaa tacagtatcc agtgatacta ttcacatggt
    24841 taattattgg ctcattgcag gtttgacgac tattatttca atgactagta cattaggtgc
    24901 ctttggagtt atggtgtccg acaaagaaaa aaaattaaat gaagatttta aggtcagtcc
    24961 tatatcaaat atcaaaattg aattgtccta tgctatattt gccgttttat ttggtattgt
    25021 catgacgatg ttctcttgca tctttgctat cggtattttt aacggcttta attccttgct
    25081 agagtattca gctatggatt atttaactat tttagggatc gtttccttag ggactgtttt
    25141 atctgcggcg atgattttac ctattttagc ctttattcat actagttcag cttttacgac
    25201 tcttagcaca attgtaggga cttttattgg ttttatttca ggagtatatc tctcaattgg
    25261 ctcggtagga aaagcattac aacaggttat gacatggttt ccattgactc aaatcaattc
    25321 tcttttgaaa caggtcttaa tgaagggttc tattgcgaag gtatttgaca aagccaacga
    25381 agccactgtc tctaactata aagaatcata tggtgttgtt ttgcgtaatg ctgatggaga
    25441 aaggctgagt aatcacttta tgttgattta tatcattgcc ctcattctta ttttattggc
    25501 aattcatttt attattaaaa aggttaaaaa atgaaagatg ataataaatt aaagatttct
    25561 gaagcttcct tagaagatta ttctgaagtg gttcatttat ttaataggaa tcatgtttat
    25621 caatttccag acggtaggcc tttaactgtt gatgaccttg atttaacgtt aaaagttaaa
    25681 gaagtgacac atttattttt actgaaaaat catggtgttt tgattggaac atcagctttc
    25741 tttaaattta ttacctatgg ttgtttagat tggaatagta gctttagtgg ttttctttta
    25801 attgattcga aaagtcgtag tggacaggca atcacttatc tatataaaac tatcttaaag
    25861 aagataacaa aactgaagtt ttctaatatc tataccgaaa tcagtaacta caataaaccc
    25921 tccttagctt tatcaaaatt gaatggcttc aaagagtatg ataagacata tgaagacata
    25981 ctgcattgtc gatcattgcg tagtcacctc cctaagatat tgaatacgtt tcgtatttcg
    26041 aattattatg gtaaaacata cgacatatca acttttcaaa ttatggagga aattgaaaat
    26101 cccttggaag aggagacaga aattaggacc aaagtctctg atgaagaaat attatttaaa
    26161 gcagaagata gtgcttcact tccttattat ttaaaaatga gcttgtttca aatggaaatt
    26221 gctaagttag ataaccgtta tgttttacaa gttgactttt tatcggaaca agtaaagagg
    26281 gttcgggtta aaactggaaa atatcacttg gcaaacttaa cgagggctca ccctagccta
    26341 actttaagtc gttttgccaa ttattattac attcaagcaa ctgtggaaac cctttatggt
    26401 aatatcgatg ttcaactgga acgtcgaaaa aagcattata gagatgcaac tatttgtctt
    26461 aagagaactt ttcaaggtta tgatttactt atctctccaa atggcagcct tatttttgaa
    26521 aagcagaaaa gaaagatact tgaagatagt tttttgattt ttagccaacc tttggacaag
    26581 aaattagttg taaaagaaga agaaaatcac attacaatca aatgtttcta tcaaggtgct
    26641 ttaattgaga aaatcatgac atttacaagt gacgaagaaa tcacttgtgt ttataaatgt
    26701 aatcaaaagg ctaaagagat gttcccaaaa ttattgaaac agactttcaa gttacactgc
    26761 caagaacagt tgattagaga tagtgaaggt tatcttgtga atgttccagg aagttacccg
    26821 attgagcatg atgattttct tcgagcagat aagtttgaag atagacaatt ccattattac
    26881 ctcccgaatg aagataaaat gatttcctat tctcctcctg gcaaagccag caatcaaatg
    26941 caatttagac ccttatgtct tattgataca gatagccttt cttttccact gacttatcat
    27001 tttaggattt cacaagcatc atcggaggag gcgttaatca atctaaaaaa acagccaata
    27061 tgggacagca actaccaagc gtcagcaact gaccttttaa aacatatctc taacctaaca
    27121 ttagaagaag agaaggatta tggcatcaag agaatgatag ctaacagaaa gcattatcct
    27181 agtcacaaac tggttttagc ctataatcag attgttttgc ctaagaatga aattccaaga
    27241 gacagtgaat tatattctat ttcttttgat tatcgaatta gaggaaagtt tgtccaaatc
    27301 cgccaagggg aacatgtcaa atatgataat aaatcctatg ttttggaaaa tagtcaacaa
    27361 ttagtattgt atgtagctag cgatgataaa tatatactaa tttctgctaa aaatggaata
    27421 ttttattctt acaaagagaa caatcattta aaaataaggt gcatgtttaa aaggaactca
    27481 ccttatgcta caaatgtgag cattacagaa tatagaaagt gtgaagaaaa atgaatacta
    27541 tttataatac attgagaaca gataaaggtt ataaagttta tgaggggtat ttatatgaaa
    27601 ttactggtga agaatgtgaa gaagccttag accttgtgat tcctaagaat attgtatttg
    27661 cagatacaga tacttgtggc tacacttttt tactcaatga agatggaaca gtttatgatg
    27721 atgtgacttt ctacaaattt gatgataaat attggttggc tagtcataaa gctttggatt
    27781 cttatttaga caacatcaat tttgactata ccgtaacaga tatttctgac gagtataaaa
    27841 tgctgcaaat tgaaggaaga tattcgggag aaattgctca gtcattttat gaatatgata
    27901 tttcaacact taattttcgt actttgatag agatgactta taaaggtgag aaaggttatc
    27961 ttgctagatt tggtttttct ggagaatttg gctatcaatt tttcctacca tcttctattt
    28021 ttgctacttt tgtttcggat gtctgtgaag gtatagcaga gtatggggat gaacttgata
    28081 gatatttaag gtttgaagtg ggacaaccca ttactgatat ttatcaacaa gaagaatatt
    28141 ctttatatga aataggttat tcttggaatc tagatttcac aaaggaagaa tttagaggtc
    28201 gcgatagctt gttagagcac atcagatcag caacagttaa aagtgttgga ttctcaacga
    28261 aggaaaaact cgcttcagga acaccagtgc tatttgatga ccaaattgtt ggaaagattt
    28321 tttggatagc agacgagaaa gactcttcgg aaaattacct aggtttgatg attgttaacc
    28381 aaacatatgc tcattcagga gttacttttg taacagaaga tggccaaatt ttgaaaacac
    28441 aatcaagccc ttattgtatc ccagaaagtt ggaacaaaga atgagcgtat atgttagtgg
    28501 aataggaatt atttcttctt tgggaaagaa ttatagcgag cataaacagc atctcttcga
    28561 cttaaaagaa ggaatttcta aacatttata taaaaatcac gactctattt tagaatctta
    28621 tacaggaagc ataactagtg acccagaggt tcctgagcaa tacaaagatg agacacgtaa
    28681 ttttaaattt gcttttaccg cttttgaaga ggctcttgct tcttcaggtg ttaatttaaa
    28741 agcttatcat aatattgctg tgtgtttagg gacctcactt gggggaaaga gtgctggtca
    28801 aaatgccttg tatcaatttg aagaaggaga gcgtcaagta gatgctagtt tattagaaaa
    28861 agcatctgtt taccatattg ctgatgaatt gatggcttat catgatattg tgggagcttc
    28921 gtatgttatt tcaaccgcct gttctgcaag taataatgcc gtaatattag gaacacaatt
    28981 acttcaagat ggcgattgtg atttagctat ttgtggtggc tgtgatgagt taagtgatat
    29041 ttctttagca ggcttcacat cactaggagc tattaataca gaaatggcat gtcagcccta
    29101 ttcttctgga aaaggaatca atttgggtga gggcgctggt tttgttgttc ttgtcaaaga
    29161 tcagtcctta gctaaatatg gaaaaattat cggtggtctt attacttcag atggttatca
    29221 tataacagca cctaagccaa caggtgaagg ggcggcacag attgcaaagc agctagtgac
    29281 tcaagcaggt attgactaca gtgagattga ctatattaac ggtcacggta caggtactca
    29341 agctaatgat aaaatggaaa aaaatatgta tggtaagttt ttcccgacaa cgacattgat
    29401 cagcagtacc aaggggcaaa cgggtcatac tctaggggct gcaggtatta tcgaattgat
    29461 taattgttta gcggcaatag aggaacagac tgtaccagca actaaaaatg agattgggat
    29521 agaaggtttt ccagaaaatt ttgtctatca tcaaaagaga gaatacccaa taagaaatgc
    29581 tttaaatttt tcgtttgatt ttggtggaaa taatagtggt gtcttattgt catctttaga
    29641 ttcacctcta gaaacattac ctgctagaga aaatcttaaa atggctatct tatcatctgt
    29701 tgcttccatt tctaagaatg aatcactttc tataacctat gaaaaagttg ctagtaattt
    29761 caacgacttt gaagcattac gctttaaagg ggctagacca cccaaaactg tcaacccagc
    29821 acaatttagg aaaatggatg atttttccaa aatggttgcc gtaacaacag ctcaagcact
    29881 aatagaaagc aatattaatc taaaaaaaca agatacttca aaagtaggaa ttgtatttac
    29941 aacactttct ggaccagttg aggttgttga aggtattgaa aagcaaatca caacagaagg
    30001 atatgcacat gtttctgctt cacgattccc gtttacagta atgaatgcag cagctggtat
    30061 gctttctatc atttttaaaa taacaggtcc tttatctgtc atttcgacaa atagtggagc
    30121 gcttgatggt atacaatatg ccaaggaaat gatgcgtaac gataatctag actatgtgat
    30181 tcttgtttct gctaatcagt ggacagacat gagttttatg tggtggcaac aattaaacta
    30241 tgatagtcaa atgtttgtcg gttctgatta ttgttcagca caagtcctct ctcgtcaagc
    30301 attggataat tctcctataa tattaggtag taaacaatta aaatatagcc ataaaacatt
    30361 cacagatgtg atgactattt ttgatgctgc gcttcaaaat ttattatcag acttaggact
    30421 aaccataaaa gatatcaaag gtttcgtttg gaatgagcgg aagaaggcag ttagttcaga
    30481 ttatgatttc ttagcgaact tgtctgagta ttataatatg ccaaaccttg cttctggtca
    30541 gtttggattt tcatctaatg gtgctggtga agaactggac tatactgtta atgaaagtat
    30601 agaaaagggc tattatttag tcctatctta ttcgatcttc ggtggtatct cttttgctat
    30661 tattgaaaaa aggtgataaa atgagtaaat ttttatttgc tcctttcatg atgaatttag
    30721 gagagagcca aagattatca aaactggcta attgtttata tgaacaagga catgatattc
    30781 atatccttgg agacaattat tatccatttt tatttaataa tgatgcttat cgctatcacc
    30841 actgtgtaga agataaatct gtttataata gtaagcgata tgatgctttc ttttctcttt
    30901 caacagactt taatttttta tcagaagaag aaattgaacg catctgttct attgaaagag
    30961 acttgttacg ccaagaaaaa tttgatgctg tattgacagg ttaccgtcta tctattgtta
    31021 caagttgtcg tttagaaagc ataccattaa tttggattat ttcaggggca acgcatatca
    31081 gtgaaattgt tgaaaactca gaaggaatat tgcctaatgg gaagataagt aaagctagta
    31141 agcctcaaac aaaggatttt atcaaaagag taatcactac ttattcaaca aatgttaaaa
    31201 cgtggaataa ctatattaaa aaatatggtg gcaaaccttt taataatgct ttagaattat
    31261 ttactggtga tctaaacttg gtaacagatt actctttgtt ttatgaattt gataaagatt
    31321 cgtcctataa aacgatagga cctattttga tagataatgt aggtttttca aaatgcagcc
    31381 aaattaatca agacaataag actgtactgc tcagctttgg tacttcattt aaacgagatt
    31441 gggtggaatc ttttttaaag acattaccaa ggcattatca ttatttactg acaagctgtg
    31501 gtgaggctat aaacattcca ggaagttata ttgagatagt tgattttatt gattttaaaa
    31561 ccatagataa agagatttat ttcgctatta tacacggtgg gcaaggaaca gtttatgcga
    31621 tggctgcaca aggtattccc tttataggta tccctttctt taacgaacaa ttttggaata
    31681 ttaagaagtt cagtaaagaa aagtgcgctt atttggtaaa gaaacctgat gtaagagaga
    31741 tacgtaaagc aattagctgt cttgaaagag actatgatat ctataaaagt tatatgataa
    31801 gactaagcca aggaattaaa gaagaagcag aggcatcttt acaaaaagcg gaggcagaaa
    31861 ttgaatcctt tatcagaaaa agacaaagtt aactatatta tgatttataa aaataggcag
    31921 tctacattgg agaataattt atctggcgat cggttgatga gatatcaaca agcaaataga
    31981 caggtaagca aagataaact gttaacaggg agttattttg tgtaccaatg cttaaaaaag
    32041 ttaggtttta ccaataagat ttgtcaggaa tctttttcta gtgttagtag ttatttaatt
    32101 ggtttgccaa aggggaaaat tagttactct aattctggtg actatcatat tctaacctat
    32161 gctcccagtg gttcaactgg ggttgatatt gagaaataca aagatagatc agaacaaacc
    32221 taccaaaact acctaggaga atcagttagt agtgatatgg attctaaatt attattttat
    32281 aaggcatggt tacaaaaaga aatttcctat aaatgtggga aatctataga tattacctat
    32341 caaacaatga tagatggcta tatttatggt tatgcttttg ataatacttt taaagtagaa
    32401 gcggtttact taaaagaatt attaccagat aactaactcg caacatgttg ttcctacata
    32461 tgcatctata aaaagcactt tgaagtattt caagggcttt ttataatacc ttgggtagtc
    32521 tttaacaatt atggttagag ttgatatgat aaactgatca tttccacatt ttatgagaaa
    32581 atcagaaaat agaggtgttt ttttgtgata ataattagaa ataataaaaa ctgaaatatt
    32641 ttctaatgta aaaaaagata aaagaactta ttgctgaaaa cgattttaac ttaacatttg
    32701 ccattgacaa gtacttagag gagtgctaaa atttcgttag ctggttaaca aaccggttaa
    32761 gtaatgtgag gttcttttat gagtgaattc ctttcagcca aacctattgg tcataatgat
    32821 tcatctaata ctattttaag aattatagag aatacgaaat atattcatca ctatgatgga
    32881 aaagttgttg tcgaaattcc tcctcaaaaa gagcctatca aagtcttttt tatggattca
    32941 cgtcttcatt atttagaatt ttcagacata tgtcggtatg taactgttgt tttgaaaaat
    33001 agtaccgttg ataatataac caatttaggg ctagataatc atattgttta tgacaatggt
    33061 gatgatatgg ttgatccgtt gccgagtatt cagcatggat tatttcttaa tgatgattaa
    33121 agatgtagac aaaaatttag aaacgaggaa gccatgacat tttataaaaa gacggacaaa
    33181 tttggattta ggaaaagtaa agtgtgccgc agcctatgcg gtgctttatt gggtacagta
    33241 gcagttgtta gtctagctac agcaagtaca gagattcacg ctgatgaagc aacaacctca
    33301 cctaccactg taacgaaagt acctcagcca gttcaagcag acacaacagc tctaaatacc
    33361 tctaagactc acagtacaca agcaactaca actcctgttg aagctaagga gaataaggtt
    33421 gtcaaaagtg aaactgttca atctgagagc cgagtaatgc ctagagataa ggtagtagaa
    33481 aggccagaaa cagtaaaagc tagtgttaac tcagacgtta gccaacctat tacaaccaca
    33541 cccccaacaa ttaatgaaaa aacagttgag attcctaact tagctcaaga tactaagaag
    33601 gtagctccaa aagtaactgt tacaccagaa gttgttaatc agccaaagca gttgcctaaa
    33661 tacgagtaag aaaactcaga gaaaggcaat aagagtacag aagcaactaa ggcagttact
    33721 gtgactaaac cacagggggc ggttgctgag aaagctactc cggctgtgcc aaagcctcaa
    33781 aaagtaagag taattgttga atttaataag gaatctatct tagattatgc gacagaacag
    33841 aaaaaaacag ttgcacagtt aaatcaagct gatgttgaga aaaaattaca aagcatcaag
    33901 caagagcaag ataaagttct taagaatatt gaaaaaagtg ttcactttga ttcaagcaaa
    33961 gtgaagcgtt acgatgctat tattaatggt gtcgcacttg atattcaagc tcaagaaatt
    34021 gaaaaattaa aaacgattgc tgatgttaga agggtctatg tctcacaaga atatgttcag
    34081 acaaaacctt tactatcttc ctcagggcaa cttattggtt tgccagaagt ttggaacaac
    34141 tcccagtata aaggtgaggg aactgttgtt gcagttattg attcaggggt tgatttcaaa
    34201 caccaagcac taaaaatcaa agaaccaaat cgtgctaagt ataataaaac tagtattgaa
    34261 aaactgattc atgaaaagaa tctcaaagga aaattctatt ccgaaaaagt accttatggt
    34321 tataactatt atgactataa tgataacctc aaggatagct acggtgtaat gcatggtatg
    34381 catgtaacag gtatcgtagg agctaatgat gataatcaaa aattgtatgg tgttgcacca
    34441 aatgctcaaa ttttagcgat gaaggtattt tcagatgacc aacaaaatcc cacaaccttc
    34501 acagatgtgt ggctaaaagc tctagacgat gctattttac taaaagctga tgttgttaat
    34561 atgagtcttg gaacacctgc tggatttgta catgagggga aagactatcc tgaacttgaa
    34621 gttattgcaa gagcgtgtaa ggcaggaatc gtcattgcag tagcagcagg taatgaagga
    34681 aatatcactg acggtaatac ttatggtgtt aagccgctag cagaaaatta tgatacagcc
    34741 ttgattgcca acccagccct cgatgataat accttggctg ttgcctctat ggagaacctt
    34801 aaaaagcatg ctcatgtttt gaaatttaaa gataaaaaat ctggaacaga agttacagaa
    34861 gtcattaatc ttcatgttgc cccaaacgct tcaaaaacta ttattggatt ggctgtagat
    34921 ttgggagcag aagctccttc ggaattgtca aaacattttg atttaagtgg taaaatcgcc
    34981 atgcttgaaa ttccagaaga taataaatct aatggatttt tagaaaaagt acaggcgatt
    35041 actaagttaa atccagctgc tatcttgctt tacaataatg cgaaggttaa agatgattta
    35101 ggcagtcaat tattagttga atcagaagca gctaaattta atattgctcg cattaccaga
    35161 agcacgtata acaatatcaa aaataatagc aataaaatta ttactatttt aacagagcgt
    35221 caagctattg ataatagttt agcaggacaa ttatcttctt attcttcttg gggacctaca
    35281 ccagacttac gtctaaaacc tgagattaca gctccaggtg gtcatatttt ctcaactgtg
    35341 gaggataatc aatatgctga taaatcagga acatcaatgg ctgcacctca ggtagctggg
    35401 gcagctgcag ttttaaaaca atacattaca gataaaaaaa ttcctgttga taatgctgct
    35461 gattttatta aattactctt gatgaacact gcacagccaa ttatcaataa acaatcaaaa
    35521 gatggcaaaa caccttactt tgtgcgccaa caaggtagcg gagcaatgaa tcttgccaaa
    35581 gctcttgtta cgacagtcgt tgcaactgta acagggacta acgataataa tgcagatggt
    35641 aagttggagt tacgtgaatt aaaagagaaa aaatttaaag cacgcattct acttcgtaac
    35701 tttggaaaaa ctaataaaac gtatatcatc tcatcagaag cgattgcgga cccagttgat
    35761 gaaaaaggat ttcgtacgca aaattcagaa cacctagtat caaaaaaagc tgatgcagtt
    35821 acaaggaaag ttactgtaga agcaggtaag acattagctg ttgaccttga tgtggattat
    35881 tctgatgcag aggctttaac tcgcaataat ttcttggaag gttatcttaa cttaaaagat
    35941 actgaaggtg tagccgattt acacttacca tttttaggtt tttacggttc atggacagag
    36001 caaaaagcta ttgatgcttt tgaaggcatt tctgaaattg gtaacggtga caaaaaacgt
    36061 cgcgttcaat tctatgttaa taaagaaacc aacaagacct catcaacctt tacaactaac
    36121 ggcatgttga gtcttccaat ctataacaat acagtatttt tctctcctaa tagtcctttc
    36181 tatgacaaag caggagttcg tattgcagct ttgcgtaata tggagtatgt tcaatattct
    36241 attatagatc cagataccaa taaagaagtt cgtgtacttg gtagatcaca cgacgttaga
    36301 aaactttatc gcctagatta tcgtaatagt tttgccatga tgccagattc tatttgggat
    36361 ggtaagatta aagattaaat tgcaaaaggg gataaacaat acatttatca aattaaagta
    36421 cagttgaata ataaaggtgt cggtggcgat ggtgtccaaa tttatcaata ctatatcaaa
    36481 atggacaaca ataaacctta cttaagtccc aaagataaga ctactgtaga gaagttagaa
    36541 gatcgctgga aaaaaattac tttcaaagtt caggatactg gcattggttt gaaagacgtt
    36601 tatcttcaat ctgttaagta tgttggtggt ggcaataata atttagacct tatcacacct
    36661 ccaggattta aaaaagaaga taaaaaagtt gaaaaaccaa aattagaccg tccaccagga
    36721 attgatttac cagcaccaac ttcaatgaga agttttgatt attcaacccc accgggaact
    36781 aagccaagca aacccaaaga tagtttatca actcctccag gtttcccaga tttaaacacg
    36841 ccgccggatg aagccaccaa aggatagtaa aaaagacgct attgaagata aatcaggagc
    36901 aattaaatat gctaagtctc ttcaacttag ctttgttgat gaccctattt tagctagcaa
    36961 agtaaatggc aaaatattac aagtcgaatc tgatggcaaa ttagtcattc ctagaaatgc
    37021 tttgtcagct aatcaatttg atgacactag tcttaaaatt tatcgtaata ataatcgcaa
    37081 taaagaaatt actatcacaa cagattattt tgcagataca aaatatgtca atatcacagc
    37141 ggttgactat ttgagcaata ctacttttga gcaattagct actggtgaaa cagtagatta
    37201 ccatgccatt gtattttcaa gctttgctgc tattaaagac aagggtggta agatttatgt
    37261 taacgataaa ttgcaagaaa cttctcgtat agcgcttaaa gataaatctg ttaagattgg
    37321 tattgaatta ccaaatgatg tcagacatat tgatagttta tctgttcgtc gtttgaatga
    37381 ggttaaaact gttgataata tcttgaaaaa tgatgaacaa gacattaatc tcagcaaaac
    37441 ttaccaatta aaatacaacc cgacaaatcg tcgtctagag tttactatta ataacattaa
    37501 ctcaagttca gaaatcatga ccactttcaa agatggaaag atgccagaat tggttgaaca
    37561 aaaagatgtt tctttggata taaacgatat ggacatgagt aagtttaaaa ctattcgact
    37621 tggacgaaag gattctgaat ttaagggaca acttattgca aaaactggaa cagttgaatt
    37681 agatatgttt ttcaaacaat ctcaagaccc agcttcaatt attaaaaaaa tataccttat
    37741 ccaaaatggt gttccaaatg aattgaaaaa atttgactct agttttggtt taactgaaag
    37801 tcagatagat ggatactata tttataaaga tgcaattaac cttaaattta aattaaccag
    37861 tggtgcaagt cttaaagttg tttataaagg gcaagaagat ccatatagtc atcagaaaga
    37921 agatatgact aaaaaaggtg aacagctcag tcattcaact caagccaatg aaaatacagc
    37981 aaaagtaacc tttgctaata ttgactggtc acattatagt aaggttactg tgaatggaaa
    38041 agaagttggt aaaggtagtg agttaccttt aactaaagga tggacaacat ttgtattaca
    38101 taaaacagaa aattcattaa atgttaaaag tttgattatg gagacgggta gtgtaagtaa
    38161 gaaagttcaa caacttcctt taagtcctag attatctaaa aataagcata tgagggatat
    38221 gctacttact atgcaaaaag attcagcgta ttacgaaaca agtgacagtc tagtccttcg
    38281 aattaatctc actgcagata ctaaacttaa ttttaatgct gttaaaggag cgagtgctct
    38341 tactgaaaat atgatgatga gacagtttgc agttgctgga ccacaagatg atcctgttag
    38401 tgaacataaa tacccatcag tatttctctt aactcctgcc ttattggaaa ctgctagtga
    38461 ggcaactcta aatggtaagg aaatcacagc atctggtatt atcggtcaca tcaaggatgg
    38521 tgataaaagc aagcatgttg aagtcaaaat ggtgaatgaa aatggagaca tgctaggaac
    38581 ccctgttatt attcaaggta aagacttgac taatcgaaca aaaccattaa tgagtggacg
    38641 tagagtactt tatgccggta aacaatatga gttccgggct aaattaccac ttagtcgttt
    38701 taacacttgg attagggttg aagtggtaac agaagcagga gagaaagcaa gtattgttcg
    38761 tcgcatgttc tttgaccaat cagttccaga gcttaacaca gcagttgcta aacgtgattt
    38821 gacttctgat actgctctta tccacatcgt tgccaaagat gactctctaa aactaaaatt
    38881 atatcaagat gattcattac ttgaatctgt tgataaaacc ggtctttata gttttagaaa
    38941 tggtgtagaa atcactaaag atatgacagt accactagaa tttggagata atattattaa
    39001 gttatctgct gttgacttat caaattatcg tcgtaatgag acccttcata tctatagaaa
    39061 ccgttttgat gttaaagcaa gccaaatgac agctgacaaa ggagctaaag taactgtgga
    39121 tatgttgatg aagcacttag ttgttccaga aatggcagga gcttatacat taacaatcga
    39181 cgaagctcca aacacaaatg aatcaggaat gttaacaaac gctaaagtat cgattcatta
    39241 tgtaaatggt ggtgttgata aagttgatgt tccgattaaa gtagttgact tagaagctat
    39301 tcgtaaagct gaagaagcac ataaagctga cgaagcacgt aaagctgaag aagcacgtaa
    39361 agctgaagaa gcacataaag ctgaagaagt acgtaaagct gaagaagcac ataaagtcga
    39421 agaagcacgt aaagctgaag agggacataa aacccaagaa gcacctatag ttgaagaagg
    39481 ctacaaagtt aataacgttc atcaaactga tactacagtt aaagcgtctg atttaccaaa
    39541 gactaagaca gtttccgcag ttcatatggc tagaacagac aataaacaga taacttcaca
    39601 tcagacacat gttgaaaaac aaattaaaaa tacattgcca tccactggtg acagcaaacg
    39661 tggttattat atcactggaa tggctatcgt tatgctgagt gtattattta gtttagctaa
    39721 aaagtttaaa agcaaatatt aatgataaaa taaaatctat ttagcaacat taattgtgat
    39781 ggataaaagg ttttatctct taaatcaaaa gtagtagctc attagctact acttttttgt
    39841 ataagtgtaa aaaagtgaat tcttaacaag aatttaacac cctacttgct atactaaaag
    39901 tgaataagaa agagaggtgt catttatgca aagaaaatcc tatttaaaat ccatgagtgt
    39961 tcttacttta acagcttgtc ttatatcagg atatgtggtt aaagatattg ctatgttaca
    40021 tgcagtatct gccagtgaga agaaagcaaa taatgtcagt ccgagagaaa atctctacag
    40081 ggctgtcaat gataattggc tagccaatac aaaactcaaa caagggcaga ctagtgttaa
    40141 tagtttttca gaaattgagg ataaattaaa gcaactgtta gtgtctgata tggctaaaat
    40201 ggcctcagga aagattgaaa caaccaatga tgaacagaaa aaaatggttg catactataa
    40261 acaaggtatg gactttaaaa caagagataa aaatggtctc aaacctctaa aaccagtttt
    40321 acaaaaactt gaagcagtct cttcaatgaa agactttcaa agtttggccc atgattttgt
    40381 gatgagtggt tttgttttac catttggttt gactgtggaa accaatgctc gagataatag
    40441 ccaaaagcaa ttggtgcttc gtcaagcacc cgcattactt gaatcacctg accaatataa
    40501 gaagggcaat aaagaaggtg aggctaaatt atcagcttac cgtacttcag caatggcttt
    40561 gcttaaacaa gctggaaaaa gtaacattga agatagaaaa ctagttaaac aagctatagc
    40621 atttgataga ctcttatcag aaaaaacgca agttgatcaa agtaaaatca cagctgaaag
    40681 tgagacagct gcggggcgat ataaccctga aagtatggaa acggttcaca attacgccaa
    40741 ggaatttgac tttaaagaat tgattgaaaa actagttggg ccaacgaata aggcagtcaa
    40801 tgtagaagat aaaacttatt ttaaacaggt taatgatgtt ataaatagta aacaattagc
    40861 caatatgaaa gcatggatga tgatttctat gctagttgat caatcagatt ttctaggaga
    40921 acaaaatcgt caagcagcga gtgcttttaa gaatgttgcg tctggtttga ctcagattga
    40981 attgaaagaa aaaatgctta cacccaatta gaatatactt ttggccatat tttgggtgat
    41041 tattatggta agaaatattt tggtgaggca gctaaaaaag acgtcgaaca tatggctaag
    41101 aaaatcatta atgtctataa aacacggtta aaaaacaaca cttggttatc agaaaataca
    41161 aaagcaatgg ccattaagaa acttgataac atgagattaa tgattggcta tccagaagat
    41221 tatcctgatc tttatcgtca gtaccaattt gatagtaaag caagcttctt tgaaaacaat
    41281 gataactaca gaaaattatc gaacaagaaa acatttgaag aatttaacca gtctaatcaa
    41341 cgtgaacatt ggcaaatgag tgccaatgct gtaaatgctt ataatgatcc taataccaat
    41401 tccatagtct ttccagcagc gatttttcaa tcaccactct acgataaaac taaaacagtt
    41461 agtcaaaatt atggagctat cggagcaatt attggtcatg aaatttcaca ctcatttgat
    41521 attaatggta tgaaatatga cgagaaaggg aatcttcacg attggtggac taaagaagat
    41581 ttaaaacatt ataagaaaaa aacacaagct atgattgacc aatgggatgg ccttaaagca
    41641 gatggcggta aagttgatgg taaattaact ttagcagaaa atattgcaga taatggtggt
    41701 gttatggcat ctctagaagc tcttaagact gaaaaaatcc aaactataaa gaattttttg
    41761 aatcatgggc aagtatttgg cgtcaaaaag caaccaaaga acaaagtaag tcctcaattc
    41821 agtcagatgt tcatgcacca tatgaattga gagctaacat cccagtacgt aatttccaag
    41881 aattttatga tgcctttggt gttaaaaaag gcgattcaat gtatctaaaa ccagaaaaac
    41941 gtttgacact ttggtaaagc tatgttttcc agacaataag gagaatgcat gaagaatata
    42001 acaaagctat caactgttgc tttaagccta ctactttgta cggcgtgtgc tgcatcaaac
    42061 acgtctacat ctaaaacaca gtctcatcat cctaaacaaa ctaaactcac agataagcaa
    42121 aaagaagaac ccaaaaacaa agaagctgct gatcaagaga tgcatcccca aggcgctgtt
    42181 gatttgacaa aatataaggc aaaaccggtc aaagattatg gtaaaaaaat cgatgttggt
    42241 gatggcaaga aaatgaacat ttatgaaact ggtcagggaa aaattccaat tgtttttatt
    42301 cctggtcaag ctgagatttc gccacgctat gcttataaga atttaattga gcggttaagt
    42361 aaaaaatata aaatttatac agtagaacca ttaggctatg gtctcagtga tattcctact
    42421 aaaccacgta cgctagaaaa cattacaaaa gaaattcaca caggcctaaa taaaattgga
    42481 gttaaaaatt tctaccttgc agctcactca cttggcggta tgtattcttt aaattatgct
    42541 aaaaattatc cagaagaagt cagaggattt attggtatgg ataccagtac accttggatg
    42601 gaaggggaac aaaaaacaaa gtatgaccct gaaagtgcta aatgggcaat gaaattacca
    42661 gatgttgatg ataagactaa cgaacagtat ctgtcaattg cgaaaaaaat taacggcaat
    42721 aagtctcaac gagatgaaga tgagcgctta ggtgataatc ttggtaaatt gaagaatgta
    42781 aaattcccta aagggatgcc aactaagtat tttcttgctg atcaatctgt ggagggaatc
    42841 aagctacgtc aagcagattt taaaggtttg aaaaattggg atcagcagca tattgattta
    42901 tctgaagatc caaaagatgc ttccgttgaa attcttaaag gtagtcacaa tattttccaa
    42961 acacaatatg aacatatggc taaagctatt gatggattta tccaatccca tcaaaagtaa
    43021 tcgttgaatg aaatacctaa gtcaaggaga aaaaatgaat aaaataacga aattatcaac
    43081 cgttgccctc gctttgatgc tttgcgttgg atgttctgcc aataaagata atcaaaaaac
    43141 taaaactgag cagcctaagc aagaaaagtc aatcgctaag caaaaacaag agagagaagc
    43201 agaaaaagct atgattgcta aaggtgctat tgacctaact aaatataaag cgaaaccaat
    43261 tgaagattac ggacaaaaaa ttgatgtcgg tgacggtaaa aaaatgaaca tatatacaat
    43321 gggtcaaggg aaaattccgc tagtagtctt ccctggtcaa gctgaaattt caccacgtta
    43381 tgcttacaaa aacttgcttg atagattagg gaaaaaatat aaaatttata cggttgaacc
    43441 gctcggttat gggcttagtg atatgacaga taagccacgt acgattgaaa atattaccaa
    43501 agaaagtcgg tgtcaaaaat ttctatctta tggctcactc gttaggtggc atatacggtc
    43561 taaattatgc taagacatat tctaacgatg tcaaaggttt cattggcatg gatacaagca
    43621 caccaaatat ggaaggtgat ggcacaactg gagaatcttc agcttcaacc ttaaaatggg
    43681 cagaatccat tccagaagtt gataagaaaa ccaatgaaca atacttatca atcggtgcta
    43741 aaaaatcagg taataaaaca gagcgcgatg aagataggcg tgttagtgac aatcttcata
    43801 aaatggaaaa agttaagttc cctaaaggta tgccagctaa gtatttctta gctaatgagt
    43861 cagcagaaga tataaaaatg cgtaaaaaaa tattccctaa gcttaaaagc tgggaacaac
    43921 aacatattga cttatctgaa gatccaaaag acgtttctgt tgagacacta gatgctagcc
    43981 acttgattta tcatacgcaa tataaacata tggcgaaggc tattgacgag tggattcaat
    44041 cgcatgaaaa ataattattc cccccaatta ttttactaag gagaaaagat gaataaaata
    44101 acgacattat caaccatcgc cctgacttta atgctttgcg ttggatgttc tgccaataaa
    44161 gataatcaaa aaactaaaac tgaggatcct aagcaagaaa acgtaactgc aaatccgaag
    44221 gaagacaagg caacagcagc caagcgaatg aagttaacga aagaacttga aaaatataat
    44281 aaaggcacag agagctttat tagtaacaaa caatatcaaa aatatatcaa aacacctatc
    44341 aaagaatatg gtcaaaaggt tgaagtcaaa ggcaagaaga tgaatgtcta tactgttgga
    44401 gaaggtaaag taccaattgt ctttattcct ggtcaaggga cggtgactgc caaacatcag
    44461 taccataatt tgattagtaa tttaagtaaa actcataagg ttgtggtagt tgagccattt
    44521 ggttcaggtt taagtgatgt cattgaccaa ccacgaaatc tagctaatat tacctcagat
    44581 atccacgaag ctttacaaaa agttggtatt accggaaaat atgtaattgc ctctcattca
    44641 attggtggtg tttatgccct taaatacatc agtacttatc caaaggaagt gctaggatta
    44701 ataggtttag atacatcaac gccgggtatg gaaggtggta agcaagttga tttcgctgct
    44761 ccagtgttaa aagaattacc taaaattcct aaagtatccg acgatattaa tgcacaattt
    44821 tttgctattg gacataagat acttaataat tctaatatga aagaagaagc gaagaattct
    44881 tctaatatga ttaacgaaag tgccaactat aagattccaa agggaattcc tgctatgtat
    44941 ttattagcac gtgaaagtga cttagggctt caattacgta gtttagatat accaattaaa
    45001 ggcagttggc aagagcaaca taaggagtta tcagaagatc caaaagaagt ctctattcat
    45061 acactagacg gaactcacca aatttatgat actaacctta aagaagtaat tgattatact
    45121 aaccaattta tgaaaacatt tgagaaataa ttgtttttag aaacagtgct ataaaatcac
    45181 taattgatag ataacgtaga agtcgataga gaatatctaa cgacttctat cgttattcta
    45241 gagattatta agatagaaaa aggagggaaa atagtgaacc tatggacatt aagtttagct
    45301 tatttgaaac gtcaaaaaat gaaaacagta accttgttct tagtttttct aactataggc
    45361 acatgtttaa tcagtctgat gagtattcag cattctttgg aaaaaaatat cctaacaaaa
    45421 caagggaaaa gcatttattt aacctccaaa gaaaaggctt attggcctga acaagcatac
    45481 gaagctttga aaaaagcaaa aatggttgag tcagtagagg caagtttaag cattgatgtt
    45541 ggtagccgtt taaaatctgt atcaccatat aactcttcta aagaagagaa tcaagttaca
    45601 ttagcaggtt atcaaagtac agaagattta cgagcttttc agacaaaagc attggtctta
    45661 aagaaaggaa gccatttagc ggccgataat acaaaacaag tactagttcc attgaagtta
    45721 gcacaaaaga atcatttaag tgtgggtaac aaattgagat taggcaaaga aaatgttact
    45781 attgcaggaa tttatgatgc gaatagtgct aagtctaaaa atacttttaa tcccaatatt
    45841 gataatacat tgattgctca ggcaacattg gttaggaaga ttagtaaaca aaaaggttat
    45901 cagacggttg cagtgcgctt aagtgataaa cgtttagtgg atacagttat ccaaaacatc
    45961 aaacaatggc cgcttgattt cgggaaatta gatgttcaaa cagctaaaga gttttatgga
    46021 gattcttatc gtaatatcga gacattgcat cgtctagtgg gacgaatcat tcttattgtt
    46081 agtctcgtgg cgatggcaat attagttgtc atgctaacat tttggattaa taaccgcatt
    46141 aaagagacgg gaattttact cgctatcgga aaaactaagt ttgaaatcat aggtcattat
    46201 ctgatagaag tactgttagt agcaggtgct gccttcacac tatctattat tggtggtgtc
    46261 tttcttggaa aaacatttgc agctggcctt ttatcacaag ttaatggtgg tgtgagttca
    46321 caaattgtac aaaatagtag tctaattatt gatagaattg ataacctcgc tgtttctgtt
    46381 ggagtaatgg atgtctttag actctatgct cagggtgctt taatttgctt atttgcagtt
    46441 gtattatcta gttattctat tcttaaacta caaccaaagc aaatattgtc acgaatgtca
    46501 taggaggtta atatgaattt atttaaacgt tcctttttat atgttagccg taaaaagcgt
    46561 aagagtatca ctttattcgt atgtttatgg ctagttgctt ctacattaat tagcggtatt
    46621 gcggtaaaaa atgctggtct tactgctaag aagacatttt caaggcaaac tggatcaatt
    46681 ttgcatattt catcggatag tactgacctt gttggtgatg gttacggatc tggagaaatc
    46741 cctgaaaaag caatagttaa catagcaagt aatccaaatg ttaagcgcgt taataataat
    46801 ttgatggctt atgctggact aacctcggaa aaaatggtta cacgaccaaa tgataaagaa
    46861 caatataaag aacaagtttt acaagttcat ggtaatagtt actccgatac agatcctaaa
    46921 tacacagcgg ggatgatttc tcttaaggga gggcgacata tcacgccgaa agatcagcga
    46981 gttataattg ttcatcaggc ttttgcgaag acaaatcatc ttaaagttgg cgataagtta
    47041 atgttttcaa aagaccccgt tagaaacagt aaggataaaa cattagttga agcaactatt
    47101 gttggcatat ataaaggtaa aacggtacaa aaatcaaatt acccttatga gatgctagaa
    47161 aatacagcct tttcagatac gcaattgatt aaggatttat acggctattc taaaggtaag
    47221 gccttttatc gccaaacaac tgtatatcct aaaaagaatg ctgatataga aactcttaaa
    47281 agttttatca aaaaacaatc ctattgattg gaataaatat caactgacaa ttacagcaac
    47341 cttgctaaca agttatgcaa aaagtataga tgtcttgaat caattaatta cttctctaca
    47401 aggcggcatg ttagtagtat ctattatcct agtgatgatg gctctgctat tctggattgg
    47461 tggtagaacc catgagacag gtattttatt agctattggt aagtctaaag caattatcat
    47521 tgtcagtacg cagtagaagt actaatgatt gctattccag cctttgcttt atcagtactt
    47581 atcagtcaat ttattggtca aaaattagca gactccttag tgtatcaggc ctctagaact
    47641 gtcagagcag actttatgaa agggtttggg aatatgcagc ttggggctga tagtgataca
    47701 gatatgttta tgcaaacgat taagacgtta aatgtacaag tgaagcctga ggaagtctgg
    47761 ttagtaattc tagttggttt agcaatcgtc ttatttgcta ttattactac tagtttaatt
    47821 attgctcgct acaaaccaag gggaatctta tcaaaactgt cctaaagtgc cataagtata
    47881 ccgaccaata gtgacatcta tattaacggt taaaatgaat atgctctgct attgaataca
    47941 tagctcaaga aagagaaatc tatgctcgaa ttaaaaaata ttgcttatcg ttataagggg
    48001 aacgacaata aaacattaga aaatatc
//
LOCUS       DATRUK010000009        47738 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.009, whole genome shotgun sequence.
ACCESSION   DATRUK010000009 DATRUK010000000
VERSION     DATRUK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47738
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.009"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..329)
                     /gene="dhaS"
                     /locus_tag="VBE34_000436"
     CDS             complement(<1..329)
                     /gene="dhaS"
                     /locus_tag="VBE34_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEO7263179.1"
                     /translation="MTSSLITKKKIAKSFKRLFISQAFDKISVSDIMEDAGIRRQTFY
                     NHFVDKYALLEWIFQTELSEQVTDNLDYISGFQLLSELLTFFKMNQEFYIKLFQIEDQ
                     NDFSSYF"
     gene            503..1492
                     /gene="dhaK"
                     /locus_tag="VBE34_000437"
     CDS             503..1492
                     /gene="dhaK"
                     /locus_tag="VBE34_000437"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEO7263180.1"
                     /translation="MKKILNQPTDVVTEMLDGLAYVHNDLVHRIEGFDIIARNEEKSG
                     KVALISGGGSGHEPSHAGFVGEGMLSAAVCGAVFTSPTPDQVLEAIKEADEGAGVFMV
                     IKNYSGDIMNFEMAQDMAEMEGIEVASVVVDDDIAVEDSLYTQGKRGVAGTILVHKIL
                     GHAARHGKSLQEIKAIADELVPNIHTVGLALSGATVPEVGKPGFVLAEDEIEFGIGIH
                     GEPGYRKEKMQPSKALATELVDKLIESFDAKSGEKYGVLINGMGATPLMEQYVFANDV
                     AKLLEDKGIEVNYKKLGNYMTSIDMAGLSLTLIKLENQEWLEALNSDVTTIAW"
     gene            1530..2108
                     /gene="dhaL"
                     /locus_tag="VBE34_000438"
     CDS             1530..2108
                     /gene="dhaL"
                     /locus_tag="VBE34_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEO7263181.1"
                     /translation="MEVKTAIEWMHTFNQKIQSNKDYLSELDTPIGDGDHGGNMARGM
                     TAVIENLDNNEFSSAADVFKTVSMQLLSKVGGASGPLYGSAFMGITKAEQSESTISEA
                     LGAGLEMIQKRGKAELNEKTMVDVWHGVIEAIEKNELTEDRIDSLVDATKGMKATKGR
                     ASYVGERSVGHIDPGSFSSGLLFKALLEVGGV"
     gene            2108..2482
                     /locus_tag="VBE34_000439"
     CDS             2108..2482
                     /locus_tag="VBE34_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEO7263182.1"
                     /translation="MSDIGIIVVSHSKNIAQGVVDLISEVAKDVSITYVGGTEDGEIG
                     TSFDQVQQIVEQNDKKTLLAFFDLGSAKMNLELVADFSEKNIIINSVPVVEGAYTAAA
                     LLQAGADLDSIQSQLAELTINK"
     gene            2492..3205
                     /locus_tag="VBE34_000440"
     CDS             2492..3205
                     /locus_tag="VBE34_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO7263183.1"
                     /translation="MTQFLGEFLGTFILVLLGDGVVAGNVLSKTKEEGTGWTAIVFGW
                     GIACTVAVYVSGLFSPAHLNPAVTLAMASIGAISWGQVIPFIIAQMLGAMVAATILWL
                     HYYPHWKETKDSGLILASFSTGPAIRHTPSNLLGEIIGTAILVITIMAIGPSKVAAGL
                     DPIIVGIVIFAVGFSLGPTTGYAINPARDLGPRLMHAILPIENKGNSDWSYAWIPVVG
                     PIIGGVLGAILYNIALTLV"
     gene            complement(3470..3874)
                     /locus_tag="VBE34_000441"
     CDS             complement(3470..3874)
                     /locus_tag="VBE34_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO7263184.1"
                     /translation="MKGFHGNIEKLTLGNTNFRQVLYTAEHCQLVLMTLPVGGEIGSE
                     IHAENDQFFRFEAGHGKVVIDGNEYEVADGDAIIVPAGAEHNVINTSETEMLKLYTIY
                     SPAHHKDGIIRATREEAEENEEDFDGVTTETK"
     gene            complement(4013..4727)
                     /locus_tag="VBE34_000442"
                     /pseudo
     CDS             complement(4013..4727)
                     /locus_tag="VBE34_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859560.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(4820..5929)
                     /locus_tag="VBE34_000443"
     CDS             complement(4820..5929)
                     /locus_tag="VBE34_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEO7263185.1"
                     /translation="MTVTGIVAEFNPFHNGHKYLLEQAQGIKVIAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVIELPFLVSVQSADYFASGAVSILARLGVDNLCFGTEEMLD
                     YARIGDIYVNKKEEMEAFLKKQSDSLSYPQKMQAMWQEFAGITFSGQTPNHILGLAYT
                     KAASQNGIRLNPIQRQGAGYHSSEKTEIFASATSLRKHQSDRFFVEKGMPNSDLFLNS
                     PQVVWQDYFSLLKYQIMTHSDLTQIYQVNEEIANRIKSQIRYVETVDELVDKVATKRY
                     TKARIRRLLTYILINAVESPIPNAIHVLGFTQKGQQHLKSVKKSVDIVTRIGSQTWDS
                     LTQRADSVYQMGNANIAEQTWGRIPFYQPVSQSDL"
     gene            complement(5926..6177)
                     /locus_tag="VBE34_000444"
     CDS             complement(5926..6177)
                     /locus_tag="VBE34_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263186.1"
                     /translation="MIIFLYACLMIFGILYIIERRVSQKKSKVILELLMNPKLHLDLL
                     SALKPFEKETDRLIYLRKTYHLDIIDAVTLNNQLKEKEV"
     gene            complement(6200..6934)
                     /locus_tag="VBE34_000445"
     CDS             complement(6200..6934)
                     /locus_tag="VBE34_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7263187.1"
                     /translation="MATYETFAAVYDAVMDDTLYDKWTDFSLRHFPKGKKKLLELACG
                     TGIQSVRFAQAGYAVTGLDLSGDMLKLAKKRATSAHQSIQFIEGNMLDLSNVGKYDLI
                     TCYSDSICYMQDEVEVGDVFIEVYKALEENGVFIFDVHSTYQTDKVFPGYSYHENADD
                     FAMVWDTYEDDAPHSIVHELTFFVQEEDGRFTRHDEVHEERTYDILTYDILLEQAGFK
                     DVKVYADFEDKKPTATSARWFFVAHK"
     gene            complement(7000..7356)
                     /gene="rsfS"
                     /locus_tag="VBE34_000446"
     CDS             complement(7000..7356)
                     /gene="rsfS"
                     /locus_tag="VBE34_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEO7263188.1"
                     /translation="MTEKDLLQLVVKAADEKRAEDIVILDLQPVTSVADYFVIMSASN
                     SRQLEAIADNIREQVKGNGGDASHLEGDSKAGWVLLDLNSVVVHIFSEDERQHYNLEK
                     LWHEAPLLDAEVFMTE"
     gene            complement(7358..7879)
                     /locus_tag="VBE34_000447"
     CDS             complement(7358..7879)
                     /locus_tag="VBE34_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO7263189.1"
                     /translation="MTCALLLIDIQQGIMDKKPKHLTNFAELLDDLLLSAKGSNCEVI
                     WIRHHDKELPQGSPQWEIWEQRHLVTHHKIIDKTYNSCFKDTHLHDYLQSKHISQLIM
                     MGLQTEYCFDTSVKVAFEYGYDIFIPQGGHLTFDTPTLSGDSIKKHYENIWHHRFATM
                     VAKDSLLKLINEE"
     gene            complement(7942..8529)
                     /gene="yqeK"
                     /locus_tag="VBE34_000448"
     CDS             complement(7942..8529)
                     /gene="yqeK"
                     /locus_tag="VBE34_000448"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEO7263190.1"
                     /translation="MTYKDYTGLDRTELLSKVRHMMSDKRFNHVLGVERAAIELAERY
                     GYDKEKAGLAALLHDYAKELSDDEFLRLIDKYQLDPDLKKWGNNIWHGLVGIYKIQED
                     LAIKDQDILAAIAKHTVGSAQMSTLDKIVYVADYIEHNRDFPGVEEARELAKVDLNKA
                     VAYETARTVAFLASKAQPIYPKTIETYNAYIPYLD"
     gene            complement(8526..9158)
                     /locus_tag="VBE34_000449"
     CDS             complement(8526..9158)
                     /locus_tag="VBE34_000449"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEO7263191.1"
                     /translation="MALELLTPFTKVELEEKKRDTNRKQIGIMGGNFNPVHNAHLVVA
                     DQVRQQLCLDQVLLMPEFQPPHIDKKETIDEQHRLKMLELAIEGIDGLSIEPIEIERK
                     CISYTYDTMKLLIEKNPDVDYYFIIGADMVEYLPKWHRIDELVKMVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRQFIKSNRQPNYLLPREVLDYIRKEGLYK"
     gene            complement(9288..9605)
                     /gene="yhbY"
                     /locus_tag="VBE34_000450"
     CDS             complement(9288..9605)
                     /gene="yhbY"
                     /locus_tag="VBE34_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEO7263192.1"
                     /translation="MLTSKQRAFLKSEAHSMKPIIQIGKNGLNDQIKTSVRNALDARE
                     LIKVTLLQNTDEDIHDVAEVLEDEIGCDTVLKIGRILILYKESARKENRKISVKVKAV
                     KSN"
     gene            complement(9698..10816)
                     /gene="yqeH"
                     /locus_tag="VBE34_000451"
     CDS             complement(9698..10816)
                     /gene="yqeH"
                     /locus_tag="VBE34_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEO7263193.1"
                     /translation="MEELFCIGCGARIQTENKDAAGYTPRAALEKGLETGELYCQRCF
                     RLRHYNEITDVHITDDEFLKLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFVAGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPVDVILTSAQNHHAIKDLIDTIEKY
                     RHGRDVYVVGVTNVGKSTLINAIIREITGSRDVITTSRFPGTTLDKIEIPLDDGSYIF
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNSEQTLFLAGLARFDFISGQKQ
                     GFTAYFDNNLNLHRTKLVGADEFYTKHVGKLLTPPTGKEVSDFPKLVRHEFTIKDKMD
                     IVYSGLGWIRVKSEAENPVVVAAWAPEGVAVVLRKALI"
     gene            complement(10816..11376)
                     /locus_tag="VBE34_000452"
     CDS             complement(10816..11376)
                     /locus_tag="VBE34_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEO7263194.1"
                     /translation="MKSKIYFLEMKLSIDDYRPTFVVEAVYDLRAEDLLRHGIRAVLV
                     DLDNTLIAWNNPDGTAEVRAWLDEMTTADISVVVVSNNNHARVERAVSRFGVDFVSRA
                     MKPFTRGINMAIERYGFDRDEVIMVGDQLMTDIRASYRAGIKSVLVKPIVKSDAWNTK
                     FNRLRERRVWKKIEENYGKIVYQKGI"
     gene            complement(11460..12374)
                     /locus_tag="VBE34_000453"
     CDS             complement(11460..12374)
                     /locus_tag="VBE34_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEO7263195.1"
                     /translation="MTKKEKGTMMTLAAGLAWGISGISGQYLMSHGVHVNLLTSLRLL
                     ITGIFLLSLARSKQKEHLVAAWKQPKFLKQVLLFSIFGLVLNQYAFLRAIHLTNAGTA
                     TVLQYMAPILILSIVCILNRQRPTSFEIIAIAMAILGTYMIATHGKLGSLAITPKGLM
                     WGLGSAITYSIYILLPVKLIHEWGSTIVIGSGMFIGGILFSLVTKAWQYPLQINVMSI
                     LAYIGIIGIGTIFAYTFFLKGVSIVGAVKGSLLASVEPVSSVFLTVLVLGEIFYPIDL
                     LGMLFIFLAVTLISYKDLIALKKMKRVR"
     gene            complement(12504..13946)
                     /gene="gatB"
                     /locus_tag="VBE34_000454"
     CDS             complement(12504..13946)
                     /gene="gatB"
                     /locus_tag="VBE34_000454"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEO7263196.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPSSAHFGQEQNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHQNMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIELEDGTRKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEEFGTKAELKNLNS
                     FNNVRKGLIHEEKRQAQVLRSGGQIQQETRRFDETTGETILMRVKEGSSDYRYFPEPD
                     LPLFDISDEWIDQVRLELPEFPQERRAKYVSSFGLSSYDASQLTATKATSDFFEKAVA
                     IGGDAKQVSNWLQGEVAQFLNSESKSIEEIGLTPENLVEMIGLIADGTISSKIAKKVF
                     VHLAKNGGSAEEFVKKAGLVQISDPEVLIPIIHQVFADNEAAVIDFKSGKRNADKAFT
                     GYLMKATKGQANPQVALKLLAQELAKLKEE"
     gene            complement(13946..15412)
                     /gene="gatA"
                     /locus_tag="VBE34_000455"
     CDS             complement(13946..15412)
                     /gene="gatA"
                     /locus_tag="VBE34_000455"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEO7263197.1"
                     /translation="MSFNNQSIDQLHDFLVKKEISATELTKATLEDIHAREQAVGSFI
                     TISDEMAIAQAKEIDDKGIDADNVMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATAVEKLYAKDMIVIGKANMDEFAMGGSTETSYFKKTNNAWDHSKVPGGSSGGSAAAV
                     ASGQVRLSLGSDTGGSIRQPASFNGIVGMKPTYGRVSRFGLFAFGSSLDQIGPMSQTV
                     KENAQLLTVISGHDVRDSTSSERTVGDFTAKIGQDIQGMKIALPKEYLGEGIAQGVKE
                     TIIKAAKHLEKLGAVIEEVSLPHSKYGVAVYYIVASSEASSNLQRFDGIRYGYRTENY
                     KNLDDIYVNTRSEGFGDEVKRRIMLGTFSLSSGYYDAYYKKAGQVRSLIIQDFEKVFA
                     DYDLILGPTAPTTAFDLDSLNHDPVAMYLADILTIPVNLAGLPGISIPAGFDQGLPVG
                     MQLIGPKFSEETIYQVAAAFEATTDYHKQQPKIFGGEN"
     gene            complement(15412..15714)
                     /gene="gatC"
                     /locus_tag="VBE34_000456"
     CDS             complement(15412..15714)
                     /gene="gatC"
                     /locus_tag="VBE34_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEO7263198.1"
                     /translation="MKISEEEVRHVAHLSKLRFSDQETKEFASSLSKIVDMIELLNEV
                     DTEGVPVTTTMADRKTVMREDIAQPGHNRDDLFKNVPQHQDYYIKVPAILEDGGDA"
     gene            complement(15852..18497)
                     /locus_tag="VBE34_000457"
     CDS             complement(15852..18497)
                     /locus_tag="VBE34_000457"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEO7263199.1"
                     /translation="METKFVYHFDEGCKEMKELLGGKGANLAEMTSIGLPVPQGFTIT
                     TQACNDYYDNACHIRESILSQIDQAMAQLEVEQNKQLGSVDDPLLVSVRSGSVFSMPG
                     MMDTVLNLGLNDRSVQGLVKKTEDERFAYDSYRRFIQMFADVVTGIPKYKFDTILDRL
                     KTDKCYQDDTELTGSDLKRLVEFYKELYQKEAGEKFPQDPKRQLLLAIEAVFKSWNNP
                     RAKIYRKLNDIPETLGTAVNIQAMVFGNMGNNSGTGVAFTRNPSTGAANLFGEYLINA
                     QGEDVVAGIRTPQSISKLAEQMPIIYQEFVSVTQKLEAHYRDMQDMEFTIENGNLYML
                     QTRSGKRTAKAAIKIAVDQVNEGLISKEEAILRIEPKQLDQLLHPSFDLKSLKKAIIL
                     TTGLPASPGAAYGKVYFHAEDVVKEMKKGNPVLLVRQETSPEDIEGMVSANGIITARG
                     GMTSHAAVVARGMGKPCVAGCSQLLVDEVRREISIGHQTIKEGEMLSIDGATGNVYIG
                     QVPMAETSVDRDFEIFMKWVDENRDMMVCSNADNPRDAQKALDFGAEGIGLCRTEHMF
                     FDDERIPVVREMILADEILSRRKALERLLSFQRDDFYQIFKVLKGKACTIRLLDPPLH
                     EFLPHDKESIESMARQMGISTLAIEKRIQTLEEFNPMLGHRGCRLAITYPEIYQMQVR
                     ALVQGAILAMKEGYEAKPEIMIPLVTAHEEISIIRDLIEETIVEESKSKKINLSFPIG
                     TMIETPRACMIADDIAKFADFFSFGTNDLTQMSFGFSRDDAGKFLGEYVDKGLLKKDP
                     FQVLDQKGIGRFIEQAVRLGKEVKPNLKIGICGEHGGEPSSIEFCYQQGLHYVSCSPF
                     RIPIAKLAAAQAKIKQSRNDITIDK"
     gene            complement(18510..19340)
                     /locus_tag="VBE34_000458"
     CDS             complement(18510..19340)
                     /locus_tag="VBE34_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEO7263200.1"
                     /translation="MEDQLTIFIISDSLGETAKAIAKACLSQFPGHDDWHFQRFSYIN
                     SQERLEQVFEEASQKTVFMMFSLVDVALASYAQKRCESEHYAYVDLLTNVIQGISRIS
                     GIDPLGEPGILRRLDNDYFKRVESIEFAVKYDDGRDPRGILQADLVIIGISRTSKTPL
                     SMFLADKNIKVINIPLVPEVPVPKELRMIDSRRIIGLTNSVDHLNQVRKVRLKSLGLS
                     STANYASLERILEETRYAEEVMKNLGCPIINVSDKAIEETATIILEILKTNGQVAKNL
                     "
     gene            complement(19351..20001)
                     /locus_tag="VBE34_000459"
     CDS             complement(19351..20001)
                     /locus_tag="VBE34_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7263201.1"
                     /translation="MYKEGDLIQLNQRQERIIDIIKQFQPITGELIAEKLGVTRAALR
                     SDLAVLSMLGIIDAKPKVGYFYLGQYHASIGTSHFEKMTVSEIMGIPLTVHQKDSVYD
                     VIVHIFMEDAGCAFILDDDDFLCGVVSRKDLLKTSIGGGDLSKMPIGMVMTRMPHVTT
                     VLENESLFAAADKLVSRKVDSLPVVRHDKQYPEKFKVIGKLSKTILASLFLEIRDY"
     gene            20169..21134
                     /locus_tag="VBE34_000460"
     CDS             20169..21134
                     /locus_tag="VBE34_000460"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEO7263202.1"
                     /translation="MTIKNLTVAGSGVLGSQIAFQAAYKGMSVTIYDINDEALNKGKE
                     RIKKLAKVYQSEIETAKEAYSDKAKSIKYNKNLLPSLDHIFLSKVADSLDLIADLPNQ
                     ITFSKNLDQAVSDADLVIEAVPETVSIKEDFYKQLAKVAPSKTIFATNSSTLVPSQFA
                     DITGRPDKFLAMHFANNIWQNNIVEIMGHKGTDDEVVKEALAFSKDIGMVPLYIHKEQ
                     PGYILNSILVPFLESALALYYNKVSDSETIDKTWKLGTGAPMGPLEILDIIGIDTAYN
                     IMKNYSDTTSDPNSLHAHLAKMLKEEFIDKGRTGKAAGHGFYDYD"
     gene            complement(21181..21729)
                     /locus_tag="VBE34_000461"
     CDS             complement(21181..21729)
                     /locus_tag="VBE34_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO7263203.1"
                     /translation="MTKALISIDYTYDFVADDGKLTAGKPAQSIASAIADVTEKAYRS
                     GDYIFFAIDNHDIGDVFHPETNLFPEHNIKGTSGRNLYGPLGTLYETIKEDSRVFWID
                     KRHYSAFSGTDLDIRLRERRVDTLILTGVLTDICVLHTAIDAYNLGYKIEVPAAAVAS
                     LNDSNHQWALNHFKTVLGATIL"
     gene            complement(21796..22581)
                     /gene="codY"
                     /locus_tag="VBE34_000462"
     CDS             complement(21796..22581)
                     /gene="codY"
                     /locus_tag="VBE34_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEO7263204.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLDAELPYNTMAAQLADIIDCNAC
                     IINGGGNLLGYAMKYKTNTDRVEEFFETKQFPDYYVKSASRVYDTEANLSVDNDLSIF
                     PVETKENFQDGITTIAPIYGGGMRLGTFIIWRNDKEFSDDDLILVEIASTVVGIQLLN
                     LQTENLEENIRKQTAVTMAINTLSYSEMKAVAAILGELDGLEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFDKLKEYN"
     gene            complement(22707..23918)
                     /locus_tag="VBE34_000463"
     CDS             complement(22707..23918)
                     /locus_tag="VBE34_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7263205.1"
                     /translation="MKIFDKSMKLEHVAYDIRGPVLEEADRMRANGEKILRLNTGNPA
                     AFGFEAPDEVIRDLITNARESEGYSDSKGIFSARKAVMQYYQLQNIHVDMDDIYIVNG
                     VSEGISMSMQALLDNDDEVLVPMPDYPLWTACVSLAGGNAVHYICDEEANWYPDIDDI
                     KSKITSKTKAIVLINPNNPTGAVYPREILQEIVDIARQNDLIIFSDEVYDRLVMDGME
                     HIPIASIAEDIFTVTLSGLSKSHRICGFRVGWMVLSGPRQHVKGYIEGLNMLANMRLC
                     SNVLAQQVIQTSLGGQQSIDSMLLPGGRIYEQRNYIHKAINEIPGLSAVKPNAGLYLF
                     PKIDTDMYRIDNDEEFVLNFLKQEKVLLTHGRGFNMNTADHFRIVYLPRVDELTELQE
                     KMARFLSQYKR"
     gene            24185..24637
                     /locus_tag="VBE34_000464"
     CDS             24185..24637
                     /locus_tag="VBE34_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO7263206.1"
                     /translation="MSQKYERILVAIDGSTESELAFEKAVNVALRNDSELILTHVIDT
                     RALQSFATFDTYIYEKLEKEAKDVLEEYEKQAREKGADKVRQVIEFGNPKTLLAHDIP
                     EKEKVDLIMVGATGLNTFERFLIGSSSEYILRHAKVDLLIVRDPNKTM"
     gene            complement(24692..26074)
                     /locus_tag="VBE34_000465"
     CDS             complement(24692..26074)
                     /locus_tag="VBE34_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO7263207.1"
                     /translation="MAIKAVFFDIDGTLLNDRKNVQKSTIKAIRNLKDQGILVGLATG
                     RGPSFVQPFLENLGLDFAVTYNGQYIYSRSEIIYTNQLSKTTVYRLIRYAGARRREIS
                     LGTASGLLGSGIIGLGTSRLGQIVSSLVPRKWAKAIERSFKHFIRRIKPQNIDSLMVI
                     LREPIYQVVLVATEGESERIQKQFPRVKLTRSSPYSMDVISEGQSKVKGIERVGQCYG
                     FDLSEVIAFGDSDNDIEMLSQVGIGVAMGNASQQVKENARYTTADNNDDGISKALAHY
                     GLIQFEIEKTFSSRDENFNKVKSFHLLMDGETIETPRLYDSKEAGFRSDFKVEEIVEF
                     LYAASQGNQEVFDQSIRNLHLAIDKARDKVISKDHPETPLVGEVDALTDLLYLTYGSF
                     VLMGVDPKPLFDTVHEANMGKIFPDGKAHFDPVTHKILKPDDWEEHFAPEPSIRRELD
                     SQIQKSLNRK"
     gene            26143..27105
                     /locus_tag="VBE34_000466"
     CDS             26143..27105
                     /locus_tag="VBE34_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEO7263208.1"
                     /translation="MKKILVLHTGGTISMNANEKGQVMSSADNPMKYVDLSLDDLDLT
                     VVDFLNLPSPQITPHHMLDIYHYLKQHASNFDGVVITHGTDTLEETAYFLDTMILPKI
                     PIIITGAMRSTNELGSDGVYNYLSALRVANSTKAADKGVLVVMNDEIHAAKYVTKTHT
                     TNVSTFQTPTHGPLGIIMKQDLLFFKATEERVRFDLDKITGTVPIVKAYAGMGDSGII
                     SFLNSQNISGLVIEALGAGNMPPKAAQEIEELIEQGVPVVLVSRCFNGIAEPVYGYEG
                     GGAKLQESGVMFVKELNAPKARLKLLIALNAGLTGQNLKDYIEG"
     gene            complement(27140..28018)
                     /gene="aroE"
                     /locus_tag="VBE34_000467"
     CDS             complement(27140..28018)
                     /gene="aroE"
                     /locus_tag="VBE34_000467"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEO7263209.1"
                     /translation="MNSLNGETLLTGLIANPARHSLSPLMWNTSFQEKNMNYAYLTFE
                     VEEGKLTEAVRGVRALSIRGVNVSMPFKQSVIPLLDDLSPQAKLVGAVNTIVNQGGTG
                     RLVGHMTDGIGCFKALAAQGFSAKNKIITIAGIGGSGKAVAVQAAMEGVAEIRLFNRN
                     SSNYDKVIDLSDKIKKQFQIKVVVDYLENKTAFKDAIRTSHFYIDATSLGMRPLDNYS
                     LINDPEILTPNLVVVDLVYKPKETALLRFVRQNGVKHAYNGLGMLIYQGAEAFQLITN
                     QEMPISSVERVLQTEN"
     gene            complement(28116..29030)
                     /locus_tag="VBE34_000468"
     CDS             complement(28116..29030)
                     /locus_tag="VBE34_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO7263210.1"
                     /translation="MLQNIGQTGIQATRIALGCMRMSDLKGKQAEEVVGTALDLGINF
                     FDHADIYGGGLSELRFRDAIKHLNVNRDKMIIQSKCGIREGYFDFSKEYILSSVDGIL
                     ERLGTEYLDFLILHRPDVLVEPEEVAEAFTKLRAEGKVKHFGVSNQNRFQMELLQSYL
                     DEPLAVNQLQLSPAHTPMFDAGLNVNMLNKASIEHDDGIVDYCRLKRVTIQAWSPFQI
                     DLSRGLFVNHPDYKELNETIAKLAKNYNVSSEAIVIAWILRHPAKMQAIVGSMNPSRL
                     KAIDKANDIALTRKEWYDIYRSAGNILP"
     gene            complement(29321..31336)
                     /gene="recG"
                     /locus_tag="VBE34_000469"
     CDS             complement(29321..31336)
                     /gene="recG"
                     /locus_tag="VBE34_000469"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEO7263211.1"
                     /translation="MLLQSPISNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVSQGISQSTLIKAIKSAFE
                     INAHLELKENLPATLLGKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENKSETNGLSILYSKHAMETKISSLPFILTNAQKRSLDEILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSIIDELPAGRKPIITRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     YKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARRVASDIVKDNNWKENTEWALILDNLRQHSDFD"
     gene            complement(31416..32954)
                     /locus_tag="VBE34_000470"
     CDS             complement(31416..32954)
                     /locus_tag="VBE34_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7263212.1"
                     /translation="MSKQKVTATLLLSTLVLSLSSPLVTLAETINPETSLTMATASTE
                     SSSEAEKQEKTQPTDSETASPSAEGSISTEKTEIGTTETSSSNESSSSSSHQSSSNED
                     AKTSDSASTASTPSTNTTNSSQADSKPGQSTKTELKPEPTLPLVEPKITPAPSQIESV
                     QTNQNASVPALSFDDNLLSTPISPVTATPFYVEHWSGQDAYSHYLLSHRYGIKAEQLD
                     GYLKSLGIQYDSNRINGAKLLQWEKDSGLDVRAIVAIAVLESSLGTQGVAKMPGANMF
                     GYGAFDHDSSHASAYNDEEAIMLLTKNTIIKNNNSSFEIQDLKAQKLSSGQLNTVTEG
                     GVYYTDNSGTGKRRAQIMEDLDRWIDQHGGTPEIPAALKALSTASLADLPSGFSLSTA
                     VNTASYIASTYPWGECTWYVFNRAKELGYTFDPFMGNGGDWQHKAGFETTHSPKVGYA
                     VSFSPGQAGADGTYGHVAIVEEVKKDGSVLISESNAMGRGIVSYRTFSSAQAAQLTYV
                     IGHK"
     gene            complement(33047..34147)
                     /locus_tag="VBE34_000471"
     CDS             complement(33047..34147)
                     /locus_tag="VBE34_000471"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO7263213.1"
                     /translation="MISSYHRPTRALIDLEAIANNVKSVQEHIPSDKKTFAVVKANAY
                     GHGAVEVSKYIESIVDGFCVSNLDEAIELRQAGIVKMILVLGVVMPEQVILAKNENIT
                     LTVTSLEWLRLCQTSAVDLSGLEVHIKVDSGMGRIGVRQLDEGNKLISELGESGASVK
                     GIFTHFATADEADNCKFNQQLTFFKDFISGLDNCPDLVHASNSATSLWHSETIFNAVR
                     LGVVMYGLNPSGTDLDLPYPINPALSLESELVHVKQLHDGSQVGYGATYQVTGDEFVG
                     TVPIGYADGWTRDMQGFSVIVNGELCEIIGRVSMDQMTIRLPQKYTIGTKVTLIGQQG
                     SCNITTTDVAQKRQTINYEVLCLLSDRIPRYY"
     gene            complement(34144..34503)
                     /locus_tag="VBE34_000472"
     CDS             complement(34144..34503)
                     /locus_tag="VBE34_000472"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEO7263214.1"
                     /translation="MIVGHGIDLQEIEAITKAYERNQRFAERVLTEQELLLFKGISNP
                     KRQMSFLTGRWAAKEAYSKALGTGIGKVNFHDIEILSDDKGAPLITKEPFNGKSFVSI
                     SHSGNYAQASVILEEEK"
     gene            complement(34528..35535)
                     /locus_tag="VBE34_000473"
     CDS             complement(34528..35535)
                     /locus_tag="VBE34_000473"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEO7263215.1"
                     /translation="MGFHQLSDKINIEILKQKTSLDLEVSQKKLAKEEELKNIIKGED
                     QRFLVIVGPCSADNPKAVLTYAKRLAKLEAAFKDKMFLVMRVYTAKPRTNGDGYKGLV
                     HHSDKLGVFFQARKMHYDIIRETGLLTADELLYPEMLSVMDDLVSYYAIGARSVEDQG
                     HRFISSGIDAPVGMKNPTSGNLRVMFNAVYAAQNQQELFYQNKQVRTDGNLLSHVILR
                     GYHNADYRSIPNYHYENLLETITHYEETDLQNPFIVVDTNHDNSGKQFLEQIRIVKSV
                     LADRQWHTKIRNYVRGFLIESYLEDGRQDKPDVFGKSITDPCLGWDKTEMLIRDIYNS
                     L"
     gene            complement(35661..38189)
                     /gene="secA"
                     /locus_tag="VBE34_000474"
     CDS             complement(35661..38189)
                     /gene="secA"
                     /locus_tag="VBE34_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEO7263216.1"
                     /translation="MANILRTVIENDKGELKKLDKIAKKVDSYADHMATLSDEALQAK
                     TPEFKERYQNGETLDQLLPEAFAVVREASKRVLGLYPYHVQIMGGIVLHHGDIPEMRT
                     GEGKTLTATMPVYLNAISGLGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PFEKREAYNCDITYSTNAEVGFDYLRDNMVVRQEDMVQRPLNYALVDEVDSVLIDEAR
                     TPLIVSGPVSSEMNQLYTRADMFVKTLNSDDYIIDVPTKTIGLSDTGIDKAENYFHLN
                     NLYDLENVALTHYIDNALRANYIMLLNIDYVVSEEQEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKESVPIQEESKTSASITYQNMFRMYHKLAGMTGTGKTEEEEFREIYNMRVIP
                     IPTNRPVQRIDHSDLLYPTLDSKFRAVVADVKERYEQGQPVLVGTVAVETSDLISRKL
                     VAAGVPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGTDRIKVVLERMNLAE
                     DDTVIKSKMLTRQVESAQRRVEGNNYDTRKQVLQYDDVMREQREIIYANRREVITAER
                     DLGSELKGMIKRTIKRAVDAHSRSDKNTAAEAIVNFARSALLDEEAITVSELRGLKEA
                     EIKELLYERALAVYEQQIAKLKDPEAIIEFQKVLILMVVDNQWTEHIDALDQLRNSVG
                     LRGYAQNNPIVEYQSEGFRMFQDMIGSIEFDVTRTLMKAQIHEQERERASQHATTTAE
                     QNISAQHVPMNNESPEYQGIKRNDKCPCGSGMKFKNCHGLRCLQ"
     gene            complement(38298..39245)
                     /gene="manA"
                     /locus_tag="VBE34_000475"
     CDS             complement(38298..39245)
                     /gene="manA"
                     /locus_tag="VBE34_000475"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEO7263217.1"
                     /translation="MSEPLFLEASMHDKIWGGTKLRDEFGYDIPSETTGEYWAISAHP
                     NGVSRVKNGRFKGCFLDKLYQGEKSLFGNPDDTVFPLLTKILDANDWLSVQVHPDDAY
                     ALKHEGELGKTECWYIISADEGSEIIYGHNAKTKEELRQMIESGDWEHLLTRIPVKSG
                     DFYYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRPDASGKLRDLRIEQSIDVLTI
                     GKPANTVPANMKLKHLSSTLLVSNDFFTVYKWEISGVTNFKQFAPYLLVSVLDGAGHI
                     TVDNKVYTLKKGDHFILPNDVVKWDIDGQLEIIASHVKK"
     gene            complement(39363..40244)
                     /locus_tag="VBE34_000476"
     CDS             complement(39363..40244)
                     /locus_tag="VBE34_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO7263218.1"
                     /translation="MTKLYGSIEAGGTKFVCAVGDEELKVVEKMQFPTTTPQETIKKT
                     VDFFKRFEKKLEAVAIGSFGPIDIDKKSKTYGYITTTPKLHWANVDLLGLISKDFNVP
                     FYFTTDVNSSAYGEVIARNNIDSLVYYTIGTGIGAGAIQKGEFIGGTGHTEAGHTYMA
                     MHPQDQANDFKGICPFHNSCLEGLASGPTLEARTGIRGELIEENSMVWDVQAYYIAQA
                     AIQATVLYRPQVIVFGGGVMAQEHMLRRVRQTFATLLNGYLPVPDLSDYIVTPAIEEN
                     GSATLGNFALAKKISKG"
     gene            complement(40312..42231)
                     /locus_tag="VBE34_000477"
     CDS             complement(40312..42231)
                     /locus_tag="VBE34_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO7263219.1"
                     /translation="MASNTEIAKQVINAIGGASNVRSVAHCATRLRIMVKDETVIDKN
                     TVENIEKVQGAFFNSGQYQIIFGTGTVNKIYDEVVAQGLPTLSTSDQKAEAAKQGNAF
                     QRAIRTFGDVFVPLLPAIVATGLFMGIRGAINNDTVLALFGTTSKAFSSSNFYTYTVV
                     LTDTAFAFFPALISWSAFRVFGGNPVIGLVLGLMMVNSALPNAWAVASGDAHPIKFFG
                     FIPVVGYQNSVLPAFFVGLLGAKLEKWLHKKIPDVLDLLLVPFLTFTVMSILALFVIG
                     PIFHSVENYVLAGTKFVLNLPLGLSGLILGGVHQIIVVTGVHHIFNLLEAQLIAADGK
                     DPFNAIITAAMTAQAGATLAVGVKTKNKKLKALAFPAALSAGLGITEPAIFGVNLRFG
                     KPFIMGLIAGAAGGWLASILKLAGTGFGITIIPGTLLYLNGQIVKYLIMVIGTTSLAF
                     VLTYMFGYEDKDEKAVAEVSPLVEETDDDPTITQTSQLRAETIVSPLDGQVIALDTVS
                     DPVFSSGIMGDGLAIKPRGNTIYSPVDGFVQIAFETGHAYGIKSDKGAEILIHIGIDT
                     VTMNGTGFTSKVKADQKVKKGDILGTFDSAKIAEAGLDNTAMIIVTNTADFADVIPLV
                     KEGVVTTGDMIIDVK"
     gene            42416..43855
                     /locus_tag="VBE34_000478"
     CDS             42416..43855
                     /locus_tag="VBE34_000478"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEO7263220.1"
                     /translation="MNLPTEIRYRPYDEWTEEDKENILKNVSKSPWRATYHLEAKTGL
                     LNDPNGFSYFNGKFHLFYQNWPFGAAHGLKQWVHTESDDLVHFKETGIKLKPDHVNDS
                     HGAYSGSALAIDDKLFLFYTGNVRDMKWNRDPRQIGAWMTSDGKITKFDKVLISQPND
                     VTEHFRDPQIFNYDNQFYAVIGAQNSKKCGFIKLYKALNNDIHHWEFVGDLDFGGTGS
                     EYMIECPNIIFVKGKPVLLYSPQGLDKNELDYQNIYPNTYKIGQYFDANSSKIVEPSP
                     IYNLDYGFEAYATQGFNTSDGRTFIVSWIGLPDIDYPSDQFDYQGAMSLVKELSIKNG
                     NLYQYPVPAMKNLRQHQAEFKTQLQTNNTYELELLVPRNDLSSFVLFANPKGQGLSIT
                     IDTAKGKVIVDRSQAGQQYATEFGTSRQCDIPKDATSINIFIDKSIFEIFINKGEKVF
                     TGRVFPDAEQSGIQLKEGHVHGKYFELKY"
     gene            43857..44819
                     /locus_tag="VBE34_000479"
     CDS             43857..44819
                     /locus_tag="VBE34_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7263221.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVTKVNEAMRTL
                     GYKPNNLARSLQGKSAKLIGLIFPNIRNIFYAELIEHLEIELFKHGYKTILCNSEKDP
                     IKEKEYLEMLGANQVDGIISSSHNLGIDDYEKVEAPIVAFDRNLAPHIPIISSDNFFG
                     GKMAAQTLKKHGCQKMIMITGNDNSDSPTGLRRLGFSYESKESKVITVTNGLSNMRRE
                     MELKSIISTHKPDGIFTSDDLTALLVIKLISQLGLSIPEDIKVIGYDGTSFIQDYVPH
                     LTTIKQPIREIAQLMVEILLAKIEGQKTNKDYILPVSLIPGSSV"
     gene            complement(44903..45337)
                     /gene="nusB"
                     /locus_tag="VBE34_000480"
     CDS             complement(44903..45337)
                     /gene="nusB"
                     /locus_tag="VBE34_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEO7263222.1"
                     /translation="MTSVFKDSRRDLRERAFQTLFSLETGGEFIDAAHFAYGYDKTVS
                     EDKVLEVPIFLLNLVNGVVDHKDELDTLISSHLKSGWSLERLTLVDKSLLRLGLYEIK
                     YFDETPDRVALNEIIEIAKKYSDETSAKFVNGLLSQFITNEN"
     gene            complement(45330..45719)
                     /locus_tag="VBE34_000481"
     CDS             complement(45330..45719)
                     /locus_tag="VBE34_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7263223.1"
                     /translation="MTTENLGEIVISPRVLEVITGIAATKVDGVHSLRNKAVTDSLSK
                     KSLGRGVYLKNEEDDTVAADIYVYLQYGVNVPAVSIAIQQAVKTAVYDMAEVKISSVN
                     IHVEGIVPEKTPKPDLKSLFDEDFLDD"
     gene            complement(45808..46368)
                     /gene="efp"
                     /locus_tag="VBE34_000482"
     CDS             complement(45808..46368)
                     /gene="efp"
                     /locus_tag="VBE34_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEO7263224.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNNETYDQYEIPTVNIENE
                     LLYILENSEVKIQFYGTEVIGVQIPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(47296..47595)
                     /locus_tag="VBE34_000483"
     CDS             complement(47296..47595)
                     /locus_tag="VBE34_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HEO7263225.1"
                     /translation="MLLPVGSVVYLIDGNQKLVIVNRGAIVEQEGQEVYFDYLGGIFP
                     EGLNLEQVYYFNQEDIDEVVFEGYHDEEEERVSRLIEKWKNTEGKNLPKGKTSVE"
BASE COUNT        15074 a         9486 c         7608 g        15570 t
ORIGIN      
        1 tcaaaatacg aagaaaaatc attttgatct tcaatttgaa ataatttaat atagaattct
       61 tgattcattt taaaaaaagt caacaactct gataacaact gaaaacctga gatatagtct
      121 aaattatccg taacttgttc gcttaattct gtttggaaaa tccattctaa taaagcatat
      181 ttatcaacaa agtgattata aaatgtttga cgtctaatac ctgcatcttc cataatatca
      241 ctcaccgata ttttgtcaaa agcttgcgaa ataaataaac gtttaaatga cttggctatt
      301 tttttcttag taataagcga agaagtcata atttctcctt tattttatat tgaattgctg
      361 atgatttatt atggtttagt gtaacatgat ttatgattat tgaaaaccct aacaaagcgg
      421 acaaacaaga aaatctgtcc attccagaaa aggcttacaa attttataat aaaagagaat
      481 taaacataaa aggagtttcc aaatgaaaaa aattctcaat caaccaactg atgttgttac
      541 tgaaatgcta gatggattag catatgttca taatgattta gtacatcgca tagaaggttt
      601 tgatatcatt gctcgcaatg aagagaagtc aggaaaagtg gctctcattt ctggcggagg
      661 aagtggacat gagccttcac atgctggttt cgtaggcgaa ggaatgttat ctgcagctgt
      721 gtgtggtgca gtatttactt ctccaactcc tgaccaagtt cttgaagcaa ttaaagaagc
      781 tgatgagggg gctggagttt tcatggttat caaaaattat tcaggggata tcatgaactt
      841 tgaaatggca caagatatgg ctgaaatgga aggcatagaa gttgcaagtg tggtcgttga
      901 tgatgatatt gcagtcgaag acagtcttta cacacaagga aaacgtggtg tagctggtac
      961 gatactcgtc cataaaattc taggacatgc tgctcgtcat ggcaaatcac ttcaggaaat
     1021 caaagctatt gctgatgaat tagtaccaaa cattcacact gttggacttg cactaagcgg
     1081 ggctactgtt cctgaagtag gcaagccagg atttgttctt gcagaagatg aaattgaatt
     1141 tggtatcggt attcatggtg aaccaggtta tcgtaaagaa aaaatgcaac cttcaaaggc
     1201 tttagcgaca gaattagttg ataagttaat tgaatcgttc gatgctaaat ctggcgaaaa
     1261 atatggtgtc cttattaacg gtatgggtgc aaccccactt atggaacaat atgtctttgc
     1321 taacgatgtt gccaaactcc ttgaggataa aggcattgaa gtgaattaca aaaaacttgg
     1381 taactatatg acttcaattg atatggctgg tttatcacta accttgatta aactggaaaa
     1441 tcaagagtgg ttagaagcac ttaatagtga cgttacaacc attgcttggt aatcattcct
     1501 gttaattaga acaaaaggaa ggaactctta tggaagtaaa aactgcaata gaatggatgc
     1561 acacttttaa ccaaaaaatt caatccaaca aggactattt atccgaacta gatactccaa
     1621 taggtgatgg cgaccatggt ggcaatatgg cgcgtggtat gactgctgtt attgaaaacc
     1681 ttgataacaa tgaatttagt agtgctgccg atgttttcaa aaccgtgtcc atgcaacttt
     1741 taagcaaagt aggcggagca tctggaccac tctatggttc tgcatttatg ggaattacaa
     1801 aagctgaaca gagtgaaagt accatttcag aggcacttgg cgcaggactt gaaatgattc
     1861 aaaaacgtgg taaagctgaa cttaatgaaa aaacaatggt tgatgtttgg cacggtgtta
     1921 ttgaagcgat tgaaaaaaat gaattgactg aagaccgtat cgattcctta gtagatgcta
     1981 caaaagggat gaaagcaaca aaaggacgcg cttcttatgt tggagaacgc tcagtaggac
     2041 acattgatcc aggatcattt tcttcaggat tattatttaa agcattacta gaagttggag
     2101 gtgtctaatg tctgatattg gtattattgt tgtttctcac tctaaaaata ttgctcaagg
     2161 agttgttgat cttatttcag aggtagcaaa ggatgtctca ataacttacg tgggtggaac
     2221 agaagatggt gagattggta cttcttttga tcaagttcaa cagattgtag agcaaaatga
     2281 taaaaagacg ttattagctt tctttgattt aggttcagca aaaatgaatt tagagttagt
     2341 agctgatttt tcagaaaaaa atattatcat taactctgtc ccagtagtgg agggagctta
     2401 caccgctgct gctcttctac aggcaggtgc tgacctggat agtattcaat cacaattagc
     2461 tgaattaact atcaataaat aaggagatat tatgactcaa tttcttgggg aatttttagg
     2521 aacatttatt ttggttcttc ttggggatgg cgtagttgca ggaaatgtgc tcagtaaaac
     2581 caaagaagaa ggaactggat ggactgctat tgtttttggc tggggaattg cttgtaccgt
     2641 tgctgtttat gtttctggat tattcagtcc cgctcatcta aatccagcag tcacattagc
     2701 aatggcttct attggagcaa tttcttgggg acaagttatc ccctttatca ttgcacagat
     2761 gttaggagca atggttgcgg caactatttt atggttacac tattatcctc attggaaaga
     2821 aacaaaagat agtggactta tccttgccag cttctcaacc ggacctgcta tccgccatac
     2881 accatcgaat ctccttggtg aaattatcgg tactgctatt cttgtgatta caattatggc
     2941 aattggtcct agtaaagttg cagcaggtct tgatccaatt atcgttggta tagttatttt
     3001 cgcagtagga ttctctcttg gtccaacaac tggttatgct attaaccctg cacgtgacct
     3061 agggcctcgt cttatgcatg ctatcttacc tattgaaaat aagggtaact cagattggtc
     3121 atacgcttgg attccagttg taggtccaat tatcggcggt gtccttgggg ctatcttata
     3181 caatattgct ttgactttag tttaactaaa aaaccagaaa gttaatactt tctggttttt
     3241 catagttgca gtatgctttc aaaatagaaa aaacaccaaa atacttagtg ttctttctgg
     3301 agattttatg aaaaaagttt aggatgttta tagtataacc gcccttactt aacgtttcct
     3361 taaaagctcg gttattaaca ttacttccta aattactatt tatattctcc aagaaatgac
     3421 tactattaag ctaaaaagcg ctattgacat agcgcttttt agtctagtat tatttagttt
     3481 cagtagtaac tccatcaaaa tcttcttcat tttcttcagc ttcttctctt gtggcacgga
     3541 taataccatc tttgtgatga gctggactgt aaattgtata gagttttaac atctcagttt
     3601 cagaagtgtt aattacgtta tgttcagcac ctgccggaac tattatagcg tctccatctg
     3661 caacttcata ttcattacca tcaatgacaa ccttaccatg accagcttca aaacggaaaa
     3721 actgatcatt ttctgcatga atctcgctac caatttctcc accaacaggc agtgtcatta
     3781 aaacgagttg acaatgttct gcggtataga ggacttggcg gaaatttgta tttcctagtg
     3841 tcaatttttc gatattccca tgaaatcctt tcataacgat acctaccttt ctatgcctac
     3901 agtctactct ttttgctcct tactgtcaaa aatttagata agagtctgac atttttaata
     3961 atatctactg ttccttgatt cctatcattt cattgcaaac ctaaaatata gcctaatgac
     4021 aatagctatc tatagcttta gaaacaaata atgctgttcc ctggcctccc caattatcaa
     4081 tagacttttg gaaacgctca tcttagatgt acattatttc aagacttgct aataaatcgt
     4141 tagtgcagtt gtaaaactga gctgtgatat aatcttgtaa aatctgaact tgtttttgaa
     4201 ctttctcatc tgtcggagaa agttctttca actgtccaaa gtctttaaaa atctgactca
     4261 tagcttgtga aatcattgaa aaatcattct tactatggct attttcaaat tctttgtaac
     4321 tatctgtatc ccccccagcg tgttctagct tcctcttgaa atacttctaa ttcttcttga
     4381 ttataagctg taaaatctga catattttta cccctctgga tagactttgc atgttcaatt
     4441 acttcttcta gtcgctgttt ctttagttcc agccacctaa tttgatcaag aagtgcttga
     4501 ttacgatcat aatttggact ttccattatc tcttttattt ttttaagagg aaattccagt
     4561 tccttaaata acaaaatttc ttgtaactta atcagtgatt catcatcata gtaacgataa
     4621 ccattctctc ctacaaatga aggagataac aaatctattt catcataata atgaagtgtg
     4681 cgcacactaa taccagaaat gtgacttatt tctttaactg ttttcataat aacctctctg
     4741 taaagagtat aatgtataac gttaagtaag ggtcaaggga aaaatattat tttgtgatga
     4801 ttaatttata aagcttaaat caaaggtcac tttgactaac aggttgataa aaagggatac
     4861 gtccccatgt ttgctctgct atattagcat ttcccatctg ataaacagaa tctgctcgtt
     4921 gcgtcaaact atcccacgtt tgtgaaccaa tcctcgtcac gatatccaca gacttcttca
     4981 cagactttaa atgctgttga cctttttgtg taaaacctaa gacatgaata gcatttggta
     5041 taggactttc cacagcattt atcaagatat aggttaataa acgtcgaatt cttgctttgg
     5101 tataccgttt agttgcgact ttatcaacta actcatccac agtctccaca taacgaattt
     5161 gactttttat acgattggct atctcttcat tcacttgata aatttgtgtc aaatcagaat
     5221 gcgtcattat ctgatatttg agtaaactaa aataatcttg ccaaactact tgtggactat
     5281 tcaaaaacaa gtcactgtta ggcatacctt tttctacaaa gaaacgatca gattgatgtt
     5341 tacgaagact tgttgcagaa gcaaatatct ctgttttttc tgatgaatga taaccagctc
     5401 cttgacgttg tatcggattt aatcgaatac cattttggct agctgctttg gtgtaggcta
     5461 agcctaaaat gtgatttggg gtttgtcccg aaaatgtaat ccctgcaaat tcttgccaca
     5521 tagcttgcat tttttgtgga taggaaagag aatccgattg ttttttcaaa aaagcttcca
     5581 tctcctcttt tttattcaca taaatatccc caatacgggc ataatcaagc atttcttcgg
     5641 taccgaagca taagttatcc acacctaatc gtgccaggat agacacagct ccactagcga
     5701 aataatccgc tgactgtacc gaaaccagaa aagggagttc aatcactaaa tcagcaccat
     5761 tttctagtgc catctgggca cgggtccatt tatctacaat cgccggctca ccacgttgca
     5821 taaaattacc cgacatggct attactttta tgccttgagc ttgttctaaa aggtacttat
     5881 gcccattatg aaagggatta aattcggcaa caattcctgt aactgtcata cttctttctc
     5941 tttcaattga ttatttaaag taacagcatc aataatatct aggtgataag ttttacgaag
     6001 gtatatgaga cgatctgttt ctttttcaaa tggttttaat gccgataata aatccaaatg
     6061 aagtttaggg ttcatcaata attccaaaat aactttagac tttttctgac ttacccgacg
     6121 ttcaataata taaagtatcc caaatatcat cagacaagcg tataagaata ttatcatctt
     6181 tttacctcca aattattact tatttatgag ctacaaaaaa ccagcgagca gaagttgcag
     6241 ttggtttttt atcttcaaaa tctgcataca ccttaacatc tttaaaacct gcctgttcaa
     6301 gtaaaatatc ataagtcaaa atatcataag tacgctcttc atgaacttca tcatgacgtg
     6361 taaatcgacc atcttcttct tgaacaaaaa atgttaattc atggacaata gaatgaggtg
     6421 catcatcttc ataagtatcc cacaccatag caaagtcatc tgcattttca tgataagaat
     6481 aaccaggaaa tactttatct gtttgataag tcgaatgaac atcaaaaata aagacgccat
     6541 tttcttctaa ggccttatag acttcgataa aaacatcacc aacctcgact tcatcttgca
     6601 tataacaaat agagtctgaa tagcacgtga tgagatcata tttaccaaca ttagataaat
     6661 ctaacatatt tccttcaata aactggatac tttggtgagc agaagttgca cgttttttgg
     6721 caagtttcag catatcgcca ctaagatcaa gtcctgtaac agcataacca gcttgtgcaa
     6781 atcgaacaga ctgaattccc gtaccacatg ctaattccaa aagttttttc ttaccttttg
     6841 gaaaatgacg aagtgaaaaa tcagtccatt tatcatataa agtatcatcc atgacagcat
     6901 cataaacagc tgcaaaagtt tcatacgttg ccatataata gttctcaaat ctttcttgat
     6961 attacatctc tcaaaaagca gtcccaagac tgcttagctt tattctgtca taaagacttc
     7021 agcatcaagc aatggtgctt catgccaaag tttttcaagg ttatagtgct gtctctcatc
     7081 ttcagagaag atatgaacca cgacgctatt gaggtcaaga agtacccaac cagctttact
     7141 atcgccttca aggtggctag catccccacc atttcctttt acttgttcac ggatattgtc
     7201 tgcgattgct tctaactgcc tactattact tgcactcata atcacaaagt aatctgcaac
     7261 actcgttaca ggctgtaaat ctaaaattac aatatcttct gctcgttttt catccgcagc
     7321 cttaacgact agctgtagta agtctttttc tgtcatttta ttcctcgttt attaatttca
     7381 ataagctatc ttttgctacc attgtcgcaa aacgatgatg ccaaatattc tcgtaatgtt
     7441 ttttaataga atccccactt aaagttggtg tatcaaatgt taggtgtcca ccttgtggaa
     7501 taaagatatc ataaccatat tcaaaagcaa cttttactga tgtatcaaaa cagtattctg
     7561 tttgaagtcc catcatgatt aattgactaa tatgcttact ttgcaagtaa tcatgtaaat
     7621 gagtatcttt aaaacaacta ttgtaagttt tatcaattat tttgtgatgc gtcacaagat
     7681 gtcgctgttc ccaaatttcc cactgtggag aaccttgagg caattcctta tcatgatgcc
     7741 ttatccatat cacttcacaa ttagaacctt tggcacttaa aagtaaatca tctaaaagct
     7801 ctgcaaaatt tgttaaatgc tttggtttct tatccataat accttgttgg atatcaatta
     7861 gtaaaagtgc acaagtcata ctttcctcca aaatgaccac tgcatcgcca aacctatcat
     7921 gaccattatt caataaaaga attagtcgag ataaggtata taagcattat aggtttcaat
     7981 cgttttagga tagataggct gagccttact tgctaaaaac gcaactgttc tagcagtctc
     8041 ataagctact gctttattaa ggtcaacttt cgctaactca cgagcttctt caactcctgg
     8101 aaaatctcga ttgtgttcaa tgtaatctgc tacataaacg attttatcca atgtggacat
     8161 ttgggctgaa ccaacagtat gttttgcaat agcagctaaa atatcttgat ctttgatagc
     8221 tagatcctct tgtattttat aaatgccaac aagaccatgc caaatattat taccccattt
     8281 ttttaggtcg ggatctagtt ggtatttatc aattaatcta agaaattcat catcagatag
     8341 ttccttagca taatcatgta ataaagcagc tagacccgct ttttccttgt cataaccata
     8401 ccgttctgct aactctattg cagcacgctc tactcctaac acatgattaa aacgtttgtc
     8461 ggacatcata tgacgcactt tactcaaaag ttcagtccga tctaaacctg tgtaatcttt
     8521 gtaggtcatt tgtaaagacc ttctttcctg atataatcta atacctctct aggcagtaaa
     8581 tagttgggct gacgattact tttaataaat tgacgaatca tacttgatga gatatccatc
     8641 aatggcagat ccacccaaat aacgggatat gatgtaccag ctttatattt tggacgttga
     8701 acaccaacaa actgcaccat tttaacaagt tcatcaatac gatgccactt aggtagatat
     8761 tccaccatat ctgccccaat aatgaaataa taatcaacat ctggattttt ttcaattaga
     8821 agcttcatcg tatcgtatgt atagctgata catttacgtt caatttcaat tggttcaata
     8881 gataaaccat caatgccttc tattgctaat tcaagcatct tgagacgatg ctgttcatca
     8941 attgtctctt ttttatcaat atgtggtggc tgaaattcag gcatcaacag aacttggtct
     9001 aaacacaatt gctgacgtac ttgatctgca acaacaaggt gggcgttatg aacagggtta
     9061 aaatttcctc ccataattcc aatttgtttt cggttagtat ctcgcttttt ctcttctaat
     9121 tcaacttttg taaatggtgt tagcaattcg agtgccataa ttcctccatg aacaagtagt
     9181 atctctaaaa aaacaaacac tgattatcat gatcagctat catacggaag tgtaacttag
     9241 gtaaatcgtt ttttagttct atactctatt tcttaatcta ttgtagttta gtttgatttt
     9301 actgctttta ctttaactga aattttacga ttttctttgc gcgctgactc tttataaaga
     9361 atcaagatac gtcctatttt aaggacagta tcgcagccaa tttcatcttc cagaacttca
     9421 gcgacatcgt ggatatcttc atcggtattt tgtaaaaggg ttactttgat tagctcacgt
     9481 gcatcgagtg cattacgtac gcttgttttg atttgatcat ttaaaccatt tttcccaatt
     9541 tgaataatgg gtttcataga atgtgcttct gattttaaga aagcacgttg tttagatgtt
     9601 aacatattag tttccttttt atctattata ggattatgac tgtatcatca gctaaattag
     9661 atgaaaaacc gtcatatgtt gctggttaat ttagttatta aattaaagct ttgcgtaaaa
     9721 caactgccac gccttctgga gcccaagcag ctactactac tggattttct gcttctgatt
     9781 tcacacgtat ccaaccaaga cccgaataaa caatatccat tttatctttg attgtaaatt
     9841 catgacgaac taatttagga aaatctgaaa cttccttacc tgttggtggt gtcaataatt
     9901 ttccaacatg tttagtgtaa aattcatctg ctccaactag tttagtgcgg tgtaaattca
     9961 ggttattatc aaaatatgcc gtaaaacctt gtttttgacc agaaataaaa tcaaaacgtg
    10021 ctaggccagc caagaataat gtttgttctg aattaagctg ataagttttg ggttttattt
    10081 cttttttagg actaacatat ttaagatttt ttgctgtcaa ataatgagcc atttgatgac
    10141 gatgaataat acccggggta tcaaaaatat agctaccatc atcaagaggt atttctattt
    10201 tatctaacgt tgtacctggg aaacgtgagg tcgtaataac atcacgacta ccagtaattt
    10261 cacgaataat agcgttaata agagttgact ttccaacatt agtaacccca acaacataaa
    10321 catctcgacc atgacgatac ttttcaatcg tatcaatcaa atccttaata gcatgatgat
    10381 tttgggcact tgtcaagatg acatcaacag gacgaagccc ctcttcatga gctctctcag
    10441 ttaaccattg tgttactttt ccatctttaa ccgatttggg aagaatatct tttttatttc
    10501 ctactaaaag aacatcgttt cctgcaacga aacgactaag accaggaata attgaaccat
    10561 tgaaatcaaa aatatcaatg acattcacca caagggcatc actatctcca acctcatgta
    10621 gtaatttcaa aaattcatca tcagtgatat gaacgtcagt aatctcatta tagtggcgca
    10681 atctgaaaca acgctgacag tataattctc ctgtctctaa acctttttca agtgctgcac
    10741 gaggtgtata gccagcagca tctttatttt cagtttgaat cctggctccg cagccaatac
    10801 aaaataactc ttccattaaa ttcctttttg atatacgatt ttaccgtaat tctcttctat
    10861 ttttttccaa acgcgacgtt cacgtaagcg attgaacttg gtattccaag catcagattt
    10921 aacaattggt tttaccaata cggactttat tcctgcgcga taactagcac gaatatctgt
    10981 catcaattga tctccaacca taatcacctc gtcacgatca aagccataac gctcaattgc
    11041 catgtttata ccacgagtaa atggtttcat cgcacgactt acaaaatcca caccaaaacg
    11101 tgatactgca cgttctacgc gagcatgatt gttattagac actactacaa ctgaaatatc
    11161 cgctgttgtc atctcatcta accatgcacg cacctcagca gtaccatctg gattattcca
    11221 agcaatgagt gtattatcta aatcaactaa aaccgcacga atgccatgac gtaataaatc
    11281 ttctgctctt aaatcataaa ctgcctcaac cacgaaggtc ggcctataat catctatact
    11341 caatttcatc tccaaaaaat atattttact cttcattcta ccataattct taagagatag
    11401 cgagtaaaga aaaaaggtct ttaaagacct ttaaattagg gtttcaaccc caaaagatac
    11461 tatcttactc gtttcatttt cttcaatgca attaaatctt tgtaagagat tagagttaca
    11521 gcaagaaaaa taaacagcat cccaagtaaa tctattggat aaaaaatttc tcctaaaaca
    11581 agtactgtca aaaataccga agaaactggc tctaccgatg ctaataagct acccttgaca
    11641 gcacctacaa tactaacccc tttaaggaaa aaagtgtaag caaaaatagt cccaatacca
    11701 ataatgccaa tataagcaag tatactcatc acattaattt gtaaggggta ttgccacgct
    11761 tttgtcacca aactaaataa aatcccacct ataaacattc ctgaaccaat aacaatcgta
    11821 cttccccatt catgaataag tttcacaggt aataaaatat aaatagaata agtaatggcg
    11881 gaaccaagcc cccacattaa tcctttcggt gtgatagcta aacttcctaa cttcccatgc
    11941 gttgcaatca tataggttcc taaaattgcc atagcaattg ctattatttc aaaagatgtt
    12001 ggtctttgtc tgtttaggat acaaacaata ctcaaaataa gtattggtgc catatattgc
    12061 aaaaccgttg cagttccagc atttgttaaa tgaatcgctc tgagaaaagc atattgattt
    12121 aaaactaaac caaatataga aaaaagtaaa acctgcttta aaaattttgg ctgtttccac
    12181 gctgctacta aatgctcctt ttgttttgat ctagctaaag acaagagaaa gatacctgtt
    12241 atcaacaatc gtaaagaagt caaaaggtta acgtgtactc cgtgcgacat caagtattga
    12301 ccactaattc cagaaatgcc ccatgctagt ccggcagcta aggtcatcat tgttcctttt
    12361 tcttttttag tcatttttcc cctcattttt atttcttaaa cactgtagtt aaagtcatct
    12421 gataaatatc ttagcttctt aattatttat ccctaggtgt tcgtgctaaa ttagcgaata
    12481 aaaaccagcc aaaaggctga tttctattct tcttttaatt ttgctaattc ttgcgctaac
    12541 agcttcagag caacttgtgg attagcttgt ccttttgttg ctttcattag ataacctgtg
    12601 aaggccttat cagcattgcg ttttcctgac ttaaagtcaa tcacagcagc ttcattatct
    12661 gcaaagactt gatgaataat tgggattaat acttcaggat ctgaaatttg aactaaacca
    12721 gcctttttaa cgaattcttc agctgaacca ccatttttag ctaaatgcac aaaaactttt
    12781 ttagcaattt tggatgaaat tgtaccgtca gcaatcaagc caatcatctc aaccaaattc
    12841 tcaggagtca gaccaatttc ttcgattgat tttgattcac tgttaagaaa ttgcgccact
    12901 tctccttgta accaattgga aacttgtttt gcatcacccc caatagcaac agctttttca
    12961 aagaaatcag aagttgcttt tgtagctgtt aattggctag catcataaga tgatagacca
    13021 aaagacgata catatttcgc acgacgttcc tgtggaaatt ctggtaattc taaacgaact
    13081 tgatcaatcc attcatccga gatatcaaat agtggtagat ctggttcagg gaaatagcga
    13141 taatctgatg agccttcctt gacacgcatc aaaatagtct cacctgttgt ttcatcaaaa
    13201 cgacgagtct cttgctgaat ttgaccacca gatcgtaaaa cttgtgcttg acgtttttct
    13261 tcatgtatca aacctttacg tacattattg aatgagttaa ggttcttaag ttctgcttta
    13321 gtaccaaact cttcttgacc ataaggacga agtgaaatat tggcatctac acgcatagaa
    13381 ccttcttcca ttttaacgtc agaaattcca gtatattgga taatctcttt gagagctgtc
    13441 aaataagcat aagcttcctc aggagaacgc atatcagctt ctgatacaat ttcaatcagt
    13501 ggaacacctt gtctattaag gtcaacatag gaataaccat ctgtcccatg tgtatttttt
    13561 cctgcatctt cttcgaggtg ggcacgctca atacgaattt tttttcgtgt accatcttct
    13621 aattcgattt caatccaacc attgtaacca ataggctcat caaactgtga aatttgataa
    13681 gctttaggat tatctggata gaaataattt ttacgatcaa aatgcatgtt ttgatggata
    13741 tccatattca aggcaagtgc cgccttgatt cctgcatcaa taactccttt attcatgact
    13801 ggaaggacac ctgggaaaga ccagtcaatt acatttgtat tagcattttg ttcctgacca
    13861 aaatgagcgg aagaaggtga gaaaatttta gagttcgtat ttaattcaac atggacttca
    13921 agtccaatga ctgtttcaaa attcattagt tctcacctcc aaaaatttta ggttgttgtt
    13981 tatgataatc tgtcgttgct tcgaaggcag cagcaacctg atatattgtt tcttcactga
    14041 atttcggacc aatcaactgc ataccaaccg gtagaccttg atcaaaacca gcagggattg
    14101 aaatacctgg taaaccagcc aaattaactg gtattgttag aatatcagct aggtacattg
    14161 caactggatc gtgatttaaa gaatctaagt caaaggctgt tgttggagct gttggaccta
    14221 aaatcaagtc ataatctgcg aaaacttttt caaaatcttg aataatcaaa ctacgaactt
    14281 gacctgcttt tttataataa gcatcgtaat acccagaaga taaactgaac gttcccagca
    14341 taatgcgacg cttaacttcg tctccaaaac cttcagatcg tgtattgaca tagatatcat
    14401 ccaagttttt ataattttct gtacggtatc cataacgaat accatcaaaa cgttgtaagt
    14461 ttgaagaagc ttccgatgaa gcaacaatat aataaacagc aacaccgtat ttagaatgcg
    14521 gtaaactaac ttcctcaata actgcaccta gtttttccag atgttttgcg gccttaataa
    14581 tcgtttcttt aactccttgc gctattcctt ctcccaaata ttcttttggt aaagcaattt
    14641 tcattccttg aatatcttga ccaattttag cagtaaaatc gcctacagta cgctcagaag
    14701 aagttgaatc tcttacatca tgcccagaaa taactgtcaa aagctgtgca ttctctttta
    14761 ctgtttgaga catcggtcca atctggtcaa gcgaactacc gaaagcaaac aaaccaaagc
    14821 gtgatacacg tccgtaagtt ggcttcatac caacgattcc attaaatgaa gccggttggc
    14881 gaattgaacc acctgtatca gagcccaatg ataaacgtac ttgtcctgaa gctactgctg
    14941 ctgcagaacc acctgatgat cctcccggaa ctttactatg gtcccaagcg ttatttgtct
    15001 ttttaaaata agatgtttct gtagagcctc ccatggcaaa ttcatccatg tttgctttac
    15061 caatcacaat catatcttta gcatataact tttcaacagc cgtagcatca aagattggct
    15121 catagttata gagcatttta gaagcagctg ttgtaagaat cccctttgtt gaaatattat
    15181 ctttaactgc taagggaata cctgacataa cattatcagc atcaattcct ttatcatcaa
    15241 tttctttggc ttgagctatt gccatctcat cagaaatagt aataaaagat ccaaccgctt
    15301 gttcacgcgc atgaatatct tccaatgtag cttttgttaa ttcagttgct gaaatttctt
    15361 ttttaacgag aaaatcatgc aactgatcaa ttgattgatt attaaacgac attaggcatc
    15421 tcctccatcc tcaagaattg caggtacttt aatataataa tcttgatgct gtggtacatt
    15481 tttaaataag tcatcacgat tatggcctgg ttgagcaata tcttcacgca tcacagtttt
    15541 gcgatctgcc atggtcgttg ttactggtac gccttctgtg tcaacttcat ttaagagttc
    15601 aatcatatca acaatctttg acaaacttga cgcaaattct ttagtctctt gatcagaaaa
    15661 gcgcaatttt gacagatggg caacatgacg cacttcttct tcagaaattt tcatgagttc
    15721 tccttatctc tgtatcattt tcgttttaag atagtctcta ctattatagc atattaaact
    15781 ctcaatgtta tcacattatc ccagcttttc ataataaacg cttcttacct atgataagaa
    15841 gcgtttactg tttatttatc tattgttata tcatttctgc tttgtttgat tttagcctgt
    15901 gcagccgcta atttagcaat aggaatccta aacggcgaac acgatacata atgtaacccc
    15961 tgttggtaac agaactcaat agatgatggt tctccaccat gctctccaca gatacctatt
    16021 ttaaggtttg gcttaacttc ttttcctaac ctcactgcct gctcaataaa ccttcctatt
    16081 cctttttgat ctaaaacttg gaaagggtct ttttttagaa gtcctttatc aacgtattct
    16141 cctaagaatt ttccagcatc atctcgtgaa aatccaaagc tcatttgtgt taaatcattg
    16201 gtcccaaaac taaagaaatc cgcaaacttt gcaatatcat ccgctatcat acaagctctt
    16261 ggtgtttcaa tcattgtccc aatagggaat gataagttaa tttttttaga tttactctcc
    16321 tcaacaattg tctcttcaat cagatcacgt attatagaga tttcttcatg cgctgtcact
    16381 aaaggaatca taatttctgg ctttgcctca tatccctctt tcattgcaag tatagcgcct
    16441 tgtacaagag ctctcacctg catttggtaa atttccggat aggtaatcgc aagtctacaa
    16501 ccacgatgac ctaacatagg gttgaattct tctaatgttt gaatccgttt ttcaatagct
    16561 aaagtagata tccccatttg tcttgccata gattctatag actctttatc atggggtaag
    16621 aattcatgta aaggagggtc aagcaaacga atcgtacaag ctttaccttt caatacttta
    16681 aaaatttgat aaaaatcgtc tctttggaaa gataaaagcc tttctagagc cttccttcgg
    16741 ctcaatattt catccgctaa aatcatttct ctaacgactg gaatacgttc atcgtcaaaa
    16801 aacatatgtt ccgtgcgaca aagacctatt ccttctgctc caaagtccaa ggccttttga
    16861 gcatctctcg gattatctgc atttgaacac accatcatat ctcgattttc atctacccat
    16921 ttcataaata tctcaaaatc acgatctacg ctagtctctg ccatcggaac ttgtcctata
    16981 taaacatttc ctgtagcacc atcgattgat aacatctcac cttcttttat cgtttgatgc
    17041 ccaatagaaa tttctcgtct tacttcatca accaataatt gactacagcc tgcaacgcat
    17101 ggtttcccca ttcctctggc tactactgcc gcatgggaag tcattccgcc tcttgctgtt
    17161 ataatcccgt ttgcactaac catcccctcg atatcttcag gtgatgtctc ttgtctgacc
    17221 aatagcactg gatttccctt tttcatctct ttaacaacat cttctgcatg gaaataaact
    17281 ttcccataag cagctccagg agaagctgga agtcctgtcg tcaaaataat agctttcttt
    17341 aaacttttca agtcaaaaga agggtgtaat aactgatcta attgtttggg ctcgatacgt
    17401 agtatagctt cttctttact aattaggcct tcatttactt gatctactgc tattttgata
    17461 gctgccttcg ctgtacgctt accgcttctc gtctgaagca tataaagatt accattttca
    17521 attgtaaact ccatatcttg catatcgcga taatgagctt ctaacttttg ggtaacgctt
    17581 acaaactctt ggtaaataat aggcatttgt tctgccaatt ttgatatgga ttgtggtgtt
    17641 ctgatacctg caacaacatc ttctccttgt gcattaatca aatactcacc aaataaattt
    17701 gctgctccag ttgatgggtt tcgtgtaaaa gcaactcccg ttccgctgtt atttcccata
    17761 ttcccaaaaa ccatagcttg gatattaacg gctgtcccaa gcgtctccgg aatatcattt
    17821 aatttacgat aaatcttagc tcttggatta ttccatgatt taaaaactgc ctcaattgct
    17881 aacaaaagct gccttttagg atcttgcgga aatttttctc ctgcttcttt ttgatatagt
    17941 tccttgtaaa attcaacaag tctctttaaa tctgaaccag ttagttctgt gtcatcttga
    18001 taacatttat ctgtcttaag tcgatctaaa atggtatcaa atttatattt tggaatgcct
    18061 gtaaccacat ctgcaaacat ttggataaaa cgacgataac tgtcataagc aaaacgctcg
    18121 tcttctgtct ttttcactaa accttggaca ctacgatcat ttaaccctaa atttaaaact
    18181 gtgtccatca taccaggcat tgaaaaaact gatcctgacc gaactgacac caacaaagga
    18241 tcatcaacac ttcctaattg cttgttctgt tcaacctcca attgtgccat tgcttgatca
    18301 atttgactaa gaatactctc tcggatatgg caagcgttat cataataatc attacaagcc
    18361 tgtgttgtaa tagtaaaacc ttgtggtacc ggtaacccaa tactcgtcat ttcagctaga
    18421 ttagccccct taccacctag cagttccttc atttccttac aaccttcatc aaaatgatac
    18481 acaaacttag tttccatcag cgtctccttt tacagatttt tagctacttg tccatttgtt
    18541 tttaagattt ctaaaataat cgttgctgtt tcttcgattg ctttatcaga tacattaatg
    18601 attgggcaac ctaaattttt cataacttct tcagcatacc tcgtctcctc caaaatccgt
    18661 tctaaactgg cataatttgc cgtactagaa agacctaagg actttaatcg aacttttcgg
    18721 acttgattaa gatgatcaac cgaattagtt agtccgataa tccttctaga atcaatcata
    18781 cgtaattctt taggaacagg aacctctgga accaaaggaa tattaataac tttaatattt
    18841 ttatctgcta aaaacatact taatggtgtt ttagaagttc ttgaaattcc tataataact
    18901 aaatctgctt gtaaaatacc tcgcggatct ctcccatcat catattttac tgcaaattct
    18961 attgattcta ctcttttgaa ataatcattg tctaagcgtc gtaaaatccc tggttctccc
    19021 aaaggatcta tacccgatat ccttgatatt ccttgaataa catttgttaa taagtcaaca
    19081 taagcataat gttcactttc acatcgtttc tgtgcatagg aagccaaagc aacatcaact
    19141 aaactaaaca tcataaatac tgtcttctgg ctagcctctt caaaaacttg ttctaagcgt
    19201 tcctgactgt taatatagga aaaacgttga aaatgccaat catcatgtcc tggaaattgt
    19261 gacaaacatg ctttcgcaat agctttggct gtttccccta aagaatctga aataataaat
    19321 atagtgagtt gatcttccat cagtttcctc ctaataatct cttatttcta aaaacaaact
    19381 agcgaggatt gttttagata atttcccgat cactttaaat ttttcgggat attgcttatc
    19441 atgacgaacg acagggagac tatccacttt tctgctcact aatttatcag ctgccgcaaa
    19501 aagactttca ttttctaaaa cagttgtcac gtgtggcata cgtgtcatca ccattcctat
    19561 tggcatttta gaaagatctc ctccgccaat actggttttt agtaaatctt tacgtgacac
    19621 gactccacag agaaaatcat catcatccaa gataaaagca caaccagcat cttccataaa
    19681 aatatgtaca ataacatcat aaactgaatc tttttgatga actgtcagag ggatccccat
    19741 aatttctgaa actgtcatct tttcaaaatg acttgtccct attgaagcat gatactgtcc
    19801 taaataaaaa taaccaacct taggttttgc atctatgata cctagcatac ttaaaaccgc
    19861 caaatcagac cttagtgctg ctcttgttac tcctaatttc tcagctatta gctcaccagt
    19921 aatgggctga aactgtttga taatatcaat aattctttct tgcctttgat ttaattgaat
    19981 aagatcacct tctttatata ttgtatacta tttattttat atgttatatt atatatcttt
    20041 ttttactgaa atcaagtaaa aaaagatact attttaaaaa taatcatact atatatttgc
    20101 ttttagacat atcttttgtt aaactttctt cgaaaacgtt ttcgaatata agtatcggag
    20161 gttgtaatat gacaattaaa aatctcactg ttgcaggaag tggcgttcta ggtagtcaaa
    20221 tcgcctttca agcagcttac aaaggaatgt ctgtcaccat atatgacatt aacgatgaag
    20281 ctttaaataa gggaaaagaa cgcatcaaaa aattagctaa agtctatcaa tcagaaattg
    20341 aaacagcaaa agaagcatat agtgataaag ctaaaagtat caagtataat aaaaacttat
    20401 tgcctagctt agaccatatt tttctttcaa aagttgccga ttcattagac ttgattgctg
    20461 atttaccaaa ccaaattact ttttcaaaaa acttagacca agctgtaagt gacgccgatt
    20521 tagtaatcga agctgtccca gaaacagtta gtataaaaga agatttttac aaacaattag
    20581 ctaaagtggc accttcaaag acaatctttg caacaaattc ttcaacactt gttccaagtc
    20641 agtttgctga tataacagga cggcctgaca aatttttagc aatgcatttc gctaacaata
    20701 tttggcaaaa taatattgtt gaaattatgg gacataaggg aactgacgac gaggttgtca
    20761 aagaagcttt ggcattctca aaagatattg gtatggtacc tttatatatt cataaagaac
    20821 aacctggcta tattcttaat tcaatactag ttccattctt agagagtgca ttagcattgt
    20881 actataataa ggtttctgat tctgaaacta ttgataaaac ctggaagtta ggaacaggag
    20941 caccgatggg acctttagaa attttagata ttattggcat cgataccgct tataatatca
    21001 tgaagaatta ttcagatacc accagcgatc ctaatagttt acatgctcac cttgcaaaaa
    21061 tgcttaaaga agaattcatt gataaaggac gtaccggtaa agctgctgga cacggctttt
    21121 atgattacga ctaaactata aaagaggtta gataaaaatc taacctcttt tataattcaa
    21181 ctaaagaata gttgcaccaa gaacagtttt aaaatgattt aaagcccatt gatgattact
    21241 atcatttaag ctggctacag cagctgctgg gacctcaatt ttataaccta agttataagc
    21301 atcaattgct gtgtgtaata cacaaatatc tgtcagtact cctgtcaata tcaaagtatc
    21361 tactcgtctt tcacgtaggc gaatatctaa atcagtacca gaaaaggcag aataatggcg
    21421 cttatctatc caaaaaacac gactatcttc cttaatagtt tcatatagag ttcccaaagg
    21481 accatatagg tttctaccag aagtcccttt aatattatgc tcaggaaaaa gattggtctc
    21541 tggatgaaaa acatctccaa tatcatgatt atcaatcgca aaaaaaatat aatcccctga
    21601 ccggtatgct ttctctgtaa cgtcagctat agctgaagcg atagattgcg ctggtttacc
    21661 tgccgttaat ttcccatcat ctgccacaaa atcatatgtg tagtctattg agatgagtgc
    21721 ttttgtcatt tttcttctta tctcctcttg atttcaaaaa aggaactgaa agctactccc
    21781 agtcccatgc tatgattaat tatattcttt taatttgtca aaaatacctt cgttaataac
    21841 tttgaggtat gttcccttca taccgagtga gcggctctca atgattcccg cagactccaa
    21901 tttacgaaga gcatttacaa taacggaacg tgtaattcca atacggtctg caataacaga
    21961 tgccgttagg cgtccttcaa gtccatctaa ttctcctaag atagcagcta cagctttcat
    22021 ttctgaataa gataaagtgt tgatagccat tgtcaccgct gtttgtttac gaatattttc
    22081 ttctagattt tctgtttgaa ggttaagtaa ttgaatacca acaacggtgc ttgcaatttc
    22141 aactaaaatc aaatcatcgt cgctaaattc tttatcatta cgccagataa taaatgttcc
    22201 caagcgcatt ccaccaccat aaattggagc gattgttgta ataccatctt ggaagttttc
    22261 ctttgtctca actgggaaaa ttgacaaatc attatctact gataagttag cttctgtatc
    22321 ataaacacga ctagcagatt taacatagta atctgggaat tgttttgttt caaaaaattc
    22381 ttccacacgg tcagtgtttg ttttatattt catggcatag ccaagtaagt taccaccacc
    22441 attaataata catgcgttac agtcaataat atcagctaat tgagccgcca ttgtattata
    22501 tggtaattcg gcatctaagc tatctacgga acgctgtaag atggatgtaa tttttctagt
    22561 tttttctaat aaattcggca tttccttcat cctttgtatg ttttgcgtta caattatatc
    22621 aaagcatttt agcttttgca aatcgatatc agtacatttt aagaacttct attgttaatt
    22681 taatgtttat ctaataaaaa ttcattctag cgcttgtatt ggcttaaaaa gcgagccatt
    22741 ttttcttgta gttcagtcaa ttcatcaaca cgtggtaagt aaacaattct aaaatggtca
    22801 gcggtattca tattaaatcc tctaccgtgt gttagtaata ctttttcttg ttttaagaaa
    22861 ttaagcacaa actcttcatc attatcaata cggtacatat ctgtatcaat tttagggaaa
    22921 agatacagtc cagcatttgg ttttacagca gataaaccag gtatttcatt aattgcttta
    22981 tggatataat tacgttgctc gtagatacga cctcctggca acaacatact atcaatagat
    23041 tgctgtccgc ctaacgatgt ttgaataacc tgttgagcaa gaacatttga acacagcctc
    23101 atgttagcta acatattcaa accttcaata taacctttaa catgttgtcg cggaccagac
    23161 aataccatcc aaccaacgcg gaaaccacag atgcgatgtg atttagataa gccagataaa
    23221 gtaactgtaa aaatatcctc tgcaatagaa gcaattggga tatgctccat tccatccata
    23281 actaaacggt cataaacctc gtctgaaaaa ataatcaagt cattttgtct agcgatatcc
    23341 acaatctctt gcagaatttc acgcggatag actgctccag ttggattatt tggattaatg
    23401 aggacgattg cttttgtttt ggatgtgatt tttgatttga tatcatcaat atcaggatac
    23461 caattagctt cctcatcaca aatataatgc acagcatttc ctccagcaag gctaacacaa
    23521 gctgtccata acgggtaatc tggcattgga actagaacct cgtcatcatt atcaagcaaa
    23581 gcttgcattg acatagagat tccctcacta acaccattaa caatgtatat atcatccatg
    23641 tcaacatgga tgttttgtaa ctggtaatac tgcataacag ccttacgagc tgaaaaaata
    23701 cctttcgaat cagagtaacc ttctgattca cgcgcattcg taattaaatc acgaatgact
    23761 tcatctggcg cttcgaagcc aaaggcagct ggatttccag tatttaaccg caatatcttt
    23821 tcaccatttg ccctcatgcg gtccgcctct tctaaaacag ggcctctaat atcataagcc
    23881 acatgttcca gcttcattga tttatcaaat atcttcataa aaatacctcg tatcttccat
    23941 tataaaacaa atattctcaa aattgaaaca tttactgcgg tttatcaagc gattaagtta
    24001 atattaagct atttcagaaa actaacataa aacgctttca tttttctaga aaaaacgtta
    24061 taatatagct ataaaatgaa taaccaacat atctaactta ctcatcactc actccttctg
    24121 ataagatggc tagttgagtg caagaggtta tctcttttgg ttatatttag aggaggacag
    24181 gcttatgtca caaaaatatg aacgcatcct tgtcgctatt gatggatcaa cagaatctga
    24241 acttgctttt gaaaaggcag ttaacgttgc tcttcgcaat gattctgaat taatcttgac
    24301 acatgttata gatacacgtg ctctacaaag ctttgcgaca tttgatactt atatctatga
    24361 aaaattagaa aaagaagcaa aagatgtcct tgaggaatat gaaaaacaag ctcgtgaaaa
    24421 aggagcagat aaagttagac aagtcattga atttggtaat cccaaaacat tacttgcaca
    24481 tgatattcca gaaaaagaaa aagtagactt gattatggtt ggtgcaacag gtcttaatac
    24541 ctttgaacgt ttccttatcg gatcttcctc agaatacatc cttcgtcacg ctaaagtaga
    24601 tttactcatt gtacgtgacc ctaataaaac aatgtaaatt taatacgctc actttctaaa
    24661 atatcctgag ttatattaac tcaggatatt tttattttcg atttaaggat ttctgaattt
    24721 ggctatctaa ttcacgtcga attgatggct caggagcgaa atgttcttcc cagtcgtctg
    24781 gttttaaaat tttatgagta acaggatcaa aatgagcttt gccatctgga aagattttac
    24841 ccatattggc ctcatgtact gtatcaaaaa gaggttttgg gtcgactccc ataagaacaa
    24901 aggagccata agtcagataa agtaaatctg ttaaggcatc cacttctccc actaatggtg
    24961 tttctggatg gtctttagaa ataaccttat ctcttgcttt atcaatagct aagtgtaaat
    25021 tacggataga ttggtcaaat acttcttggt taccttgact agcagcatac aagaactcaa
    25081 cgatttcttc tactttaaag tccgacctaa aaccagcttc cttactgtca tataagcgtg
    25141 gcgtttcaat agtttcacca tccattaata gatggaaaga ttttacttta ttgaaattct
    25201 cgtcacgact actgaatgtt ttttcaatct caaattggat aagtccataa tgggctaatg
    25261 ccttagagat accatcatca ttattgtcag cagttgtata acgtgcattt tctttcactt
    25321 gctgactagc gttccccatg gcaacaccaa taccaacttg agataacatc tcaatatcat
    25381 tatcagaatc tccaaatgct atcacttctg ataaatcaaa accatagcat tgaccaacac
    25441 gttcaattcc cttaactttt gactgccctt cagaaatgac atccattgag taaggactgc
    25501 ttcttgttaa tttaacacga ggaaattgtt tttgaattcg ctctgattcg ccctctgttg
    25561 caactaaaac gacctgataa ataggttctc gtaagataac catgaggcta tcaatatttt
    25621 gaggtttaat ccgacgaata aaatgcttaa agcttcgttc aatcgctttt gcccattttc
    25681 tcggaacaag gctagataca atctgcccca aacggctagt tcctagacca ataatacctg
    25741 aaccgagtaa tcctgaggct gttcctaatg aaatttctct tcttcttgct ccagcataac
    25801 gaatcagacg atagacagtt gtcttagata attgattggt ataaataatt tcacttctac
    25861 tatagatata ttgaccatta taggttacag caaaatctaa accaaggttt tctaaaaaag
    25921 gttgtacaaa actaggacct cgccccgttg ctaaaccgac tagtattcct tggtctttca
    25981 aatttcgaat tgctttaatt gttgactttt gtacattctt acgatcatta agaagtgtac
    26041 catcaatatc aaaaaatact gccttaattg ccactgggtc aacatcccct cttttttatg
    26101 ataaaataaa ttatatcaca acttgaaaga agtataaaat caatgaaaaa aatccttgtt
    26161 ctacacacag gtggaacgat ttccatgaat gctaacgaga agggacaggt catgtccagc
    26221 gccgataacc caatgaaata cgttgattta tcattagatg accttgattt gactgttgta
    26281 gattttctaa atcttcccag tcctcaaatc acccctcatc atatgcttga tatctatcac
    26341 tatctgaagc aacatgccag taattttgat ggagttgtca ttacgcatgg gactgacact
    26401 ttagaagaaa cagcatattt tttagacaca atgatactcc ctaaaatccc aatcatcatt
    26461 accggtgcta tgagaagcac aaatgaactt ggtagcgatg gcgtttataa ttacctatca
    26521 gccctccgtg tcgctaatag tactaaagct gccgacaagg gggtgctagt tgtcatgaat
    26581 gatgagatac atgctgctaa atatgtcaca aaaacacata caacaaacgt ttcgacattc
    26641 caaaccccta cacatggccc ccttgggatt attatgaaac aagacctgct tttctttaaa
    26701 gcaactgaag agagagttcg ttttgattta gataaaatta caggaactgt tcctatcgtg
    26761 aaagcatatg ctggcatggg tgatagtggt atcattagct ttttaaattc tcaaaatatt
    26821 agcggattag ttattgaagc actcggtgca gggaatatgc ccccaaaagc tgcacaagaa
    26881 atcgaagaat taattgagca aggtgtccct gttgttctag tgtctcgctg ttttaatggt
    26941 attgctgagc cagtttacgg atatgaaggt ggcggagcaa aattacaaga atctggtgtc
    27001 atgtttgtta aagaactaaa cgctcccaaa gccagactca aattactcat tgcactcaat
    27061 gcaggtctca cagggcaaaa tctaaaagac tatattgaag gttgataaaa aactctagaa
    27121 atatttctag agttttttat taattttcag tttgtaatac tctctcaaca ctgctaatag
    27181 gcatttcttg attcgttatt aattgaaatg cttctgctcc ttgataaatc agcatcccta
    27241 gaccattata agcatgtttc actccatttt gtctaacaaa tcgtaacaat gctgtttctt
    27301 taggcttgta aaccaagtcg acaactacta aattcggtgt taaaatttct ggatcgttaa
    27361 ttaaactata attatctaat ggcctcattc ctaaactagt agcatcaata taaaaatgac
    27421 tagttctaat agcgtcttta aatgctgtct tattttctag ataatcaacg actaccttta
    27481 tttgaaactg ttttttaatt ttatctgata agtcaatgac cttatcgtaa tttgagctgt
    27541 tacgattaaa taatctaatt tccgcaactc cctccatagc tgcttgaact gcaactgctt
    27601 tacctgaacc accaatacca gctattgtaa ttattttatt tttagcactg aaaccttgag
    27661 ctgctaaagc tttaaaacaa ccaatgccat ctgtcatatg gcctactaaa cgtccggttc
    27721 caccttgatt aacgatagta tttacagcac ccactaattt agcttgagga gataaatcat
    27781 ctagcaaagg gataacactc tgtttaaatg gcattgaaac attaacacca cgaatactca
    27841 atgccctgac acctcgaaca gcttctgtta atttaccctc ttctacttca aatgtcagat
    27901 aggcataatt catgtttttt tcttgaaaag aggtattcca cattaacggg gatagagagt
    27961 ggcgtgcagg attggcaatt agaccagtta gtaaagtttc cccatttaat gaattcattg
    28021 tataaccttc tttcttaaca atttatttca gttcatttta ccaaaaactg actcaattaa
    28081 aaagagagta tgtaatttat actctctccc ttagtttaag gcaatatatt acctgcacta
    28141 cggtaaatat cataccattc tttacgggtt aaagcgatat cattagcctt atcaatcgcc
    28201 tttaaacgag atggattcat tgaaccaaca atcgcctgca ttttggcagg atgacgtaaa
    28261 atccatgcaa tcacaatagc ttcgctgcta acattgtagt tttttgccag tttagctatg
    28321 gtttcattta attccttata atccggatga ttaacaaata aaccacgaga taaatcaatt
    28381 tgaaatggag accaagcttg aatggtaact cttttaagac gacaataatc aacaatgcca
    28441 tcatcatgtt caattgaagc tttatttaac atgttaacat ttaaccctgc atcaaacatt
    28501 ggtgtatgtg ctggcgataa ctgaagttga ttcactgcta agggctcgtc aaggtaggat
    28561 tgtaacaact ccatttgaaa ccggttttga ttgctaacac caaagtgttt tactttacct
    28621 tctgccctca gtttggtaaa tgcttctgct acttcttcag gctcaactaa tacatctgga
    28681 cgatgaagaa tgagaaaatc taaatattct gtccctaatc tttctaaaat accatctact
    28741 gatgataaaa tatactcttt agaaaaatca aagtaacctt ctcgtatccc acatttagat
    28801 tgaataatca ttttgtcacg attgacattt aaatgtttga ttgcatctcg aaagcgtaat
    28861 tcagatagcc ctcccccata aatatcagca tgatcaaaaa aattaatacc caaatctaat
    28921 gctgttccaa ctacttcttc agcttgtttt cctttcaagt cactcattct catacaacct
    28981 aaagcaattc gagttgcttg aatgcctgtt tgtccaatgt tttgtaacat atgcacccca
    29041 ttctaatctg tttgtattaa cagaaagatt aatcaattca ttataacaaa atttctatga
    29101 aaaggcttac tattacaaat gcaaaaagct gagtaaattt aactcaactt tttttctgat
    29161 actcattatt gactaaataa gtcggtaaga aaaatctatt ctgaaaagta ctattataaa
    29221 ttacagtaat cattattgtc ataacatacc tccacatctg tgataggaat atcatatgac
    29281 agcaagctta aactgagctt agcacagcct taatagaaac ttaatcaaaa tctgaatgtt
    29341 ggcgtaaatt atctagaata agtgcccatt ctgtattttc tttccaatta ttatctttga
    29401 caatatcact agcaacacgt ctggcttctt ctaaaatagg ataatcctca acgatatcag
    29461 ctacttgaaa ttctggaatt ccagactgac gagtaccaaa gatttcacca gaaccacgca
    29521 tttttaaatc cgactcagct aaaacgaaac catctgtcgt ttctgtcatg attgtcattc
    29581 gttttttccc agaatcagtt ttgggattag ccactaaaac agcgtatgat tgtttatatc
    29641 cacgaccaac acgcccacga agttgatgta gctggcttaa tccaaaacga tcggcatcca
    29701 taataatcat gatcgttgca tttgggacat ttacccctac ttcaataact gttgtagata
    29761 ctaaaatatg acttttttta tccttgaaat cttgcattat agcatctttt tcatcatttt
    29821 tcatacgtcc atgtacaaga gcaaccttag caattccttc aaaataagta gataattcag
    29881 catgcaatgc tactgcattc tttaaatcta aagcttctga ctcttcaatc aacggtgaaa
    29941 tgacatacac ttgagcatct ttttgcaatt caccttttac ccattccaac acagtaccta
    30001 gctgctcgtg tttcacccag cgtgtaataa taggtttacg accggcaggt aattcatcaa
    30061 taatagaaac atccatttct ccaaaagctg tgattgctag agttcgggga attggggtgg
    30121 ctgtcatcat taaaacatca ggattttctc ccttttcacg gaaaattcta cgctgtttaa
    30181 caccaaaacg atgttgctcg tctgtaatta caagccccag tttatggtac tgtaccgagt
    30241 cctggataag agcatgagtt cctacaatca tatcaaccga gccatttgca atagctgcta
    30301 aaaccgtacg cttaacagct gctttcatac ccgaagttaa tatagcgatt gataaatctg
    30361 gaaataactc ttgcagacta atgtagtgtt gttcagctag gatttccgtt ggaaccatca
    30421 aagccgattg aaaacctgct gtataagctg catacattga tagaccagca atgactgtct
    30481 ttcctgatcc tacatctcct tgcaataaac gattcatatg agctcccgat gacatatcag
    30541 ataatatttc atctaaagag cgcttttgag cattcgttag aataaaaggt aaagatgaaa
    30601 tctttgtctc catagcatgt ttactataaa gaatagacaa accatttgtt tcagatttat
    30661 tttcggcttt caaaacttga aggttcattt gaaagtaaaa taattcttca aatttaatcc
    30721 gacggagcgc ttgcttatac tctgtgatat cttttgggaa atgcatagct aaacaagcct
    30781 gactacgacc catcaatcgg tattttccca ataaagtagc tggtaaattt tccttcaatt
    30841 ccaaatgcgc attgatttca aaagctgact taatagcttt aatcaaagta gactgtgaaa
    30901 ttccctgaga tacgcgataa acaggttgca tgtcatcttc aacttgagct aaaaccttca
    30961 tcccagtaat agctgattta gttgcatccc atttaccaaa gacagctacc tcttgaccaa
    31021 gttctatttt atcagctaaa tagggttgat taaaaaaact aacatttaag acagcttccc
    31081 cttgacgcaa tttgaaactt aaacggttac gtttaaaacc ataatattgt acattagctg
    31141 gagtaacgac taagcctgta atgactgctt tttcaccatc cactaaatca aaaacagatt
    31201 ttgatttaaa atcttcatag cgaaacggat aataaagaag taaatcttct actgtataaa
    31261 tatctaattt ctgaaatttt tctgctgatt ttggtccaaa tcccttaaga ttagaaattg
    31321 gtgattgtaa tagcataaga ttctccttct aatatctttt attatactaa aagatcacca
    31381 aaaaggcgcc tcaagagcgc ctttattatg tctaattatt tatggccaat aacataagtt
    31441 aattgtgcag cttgtgctga actaaaagta cggtaagaga caataccacg tcccattgca
    31501 ttagattctg aaatgagaac tgaaccatct tttttaactt cttcaacaat agctacgtga
    31561 ccgtaagtgc catcagcacc agcttgtcct ggtgaaaatg atacagcata gcctactttt
    31621 ggtgaatgtg ttgtttcaaa gccagcctta tgttgccaat ctccaccatt acccataaat
    31681 ggatcaaatg tataacctaa ctctttagcg cggttaaaga cataccatgt acattcaccc
    31741 catggataag ttgatgcaat atagctagct gtgttaaccg ctgttgataa gctaaaacca
    31801 cttggtaaat ctgctaaact tgctgtcgat aaagctttca aggcagcagg aatttctggt
    31861 gtccctccat gttgatcaat ccagcggtct aaatcttcca taatctgggc acgacgttta
    31921 ccagttccag agttatctgt ataataaaca ccaccctcag taactgtatt aagttgtcca
    31981 gaagataatt tctgtgcttt caaatcttgg atttcaaagc tagagttgtt gtttttaata
    32041 attgtatttt ttgtcaacaa cataattgct tcttcatcat tataagcact agcatggcta
    32101 gagtcatgat caaaggcacc ataaccaaac atattagcac ctggcatctt agccactcct
    32161 tgagttccca atgaactttc aaggacagca atagctacaa tagcacggac atctaaacca
    32221 ctatcttttt cccattgtaa taacttagca ccattgatac gattagaatc atattgaatc
    32281 cctaaagatt ttaagtaccc atctaattgt tcagctttga taccgtaacg atgtgacaat
    32341 aaatagtgag agtaggcatc ctgaccagac cagtgttcta cgtagaatgg cgttgctgtc
    32401 actggtgaaa tcggtgttga taataagtta tcatcaaagg ataaagcagg aacagaagca
    32461 ttctgatttg tctgaacact ttctatctga gacggagcgg gagttatttt aggctctact
    32521 aatggtaagg taggctcagg ttttaattca gtctttgttg attgacctgg cttactgtct
    32581 gcttgactac tgtttgtagt attagtgcta ggagtagatg ctgttgaagc agaatcagat
    32641 gttttagcat cttcgttgga agaagattga tgtgatgaac ttgatgatga ttcattgctt
    32701 gatgatgtct ctgtcgtacc aatctctgtt ttttctgttg agatacttcc ttcggctgaa
    32761 ggtgaagcag tttctgaatc tgtaggttgt gttttttcct gtttctctgc ttcagaagaa
    32821 ctttctgttg atgctgttgc cattgtcagg cttgtttctg gattaatagt ttctgctaag
    32881 gtcactaaag gtgatgatag cgataagact aaagtggata acaacaaagt tgccgttact
    32941 ttttgttttg acatatacta atgattccct tctataaaaa ttgtcactga ttagtctaac
    33001 atatttttaa attttttaaa acagttaact actgtgtcat ttgtttttaa taatatctcg
    33061 gaatacgatc gcttaaaaga caaagtactt cataattaat cgtctgtctt ttttgagcga
    33121 cgtccgtagt tgtaatgtta caactacctt gttgaccaat caaggttact ttagttccga
    33181 tagtatattt ttgaggtaaa cggatagtca tttgatccat cgagacacgc ccaataattt
    33241 cacaaagttc tccatttact ataactgaaa atccttgcat atctcgtgtc catccatcag
    33301 cgtagccaat cggtactgtt ccaacaaatt cgtctccagt aacttgatag gttgcgccat
    33361 agccaacttg agaaccatcg tgtagttgtt taacatgtac tagctcagac tctaaactta
    33421 aagcagggtt tatagggtaa ggtaagtcta aatcagttcc acttggattt aagccgtaca
    33481 taacaacacc caatctaacg gcattaaaaa ttgtctcact atgccaaagg cttgtagcag
    33541 aattactagc atgaacaagg tccggacaat tatctaatcc tgaaataaag tctttaaaaa
    33601 aagtaagttg ttgattgaat ttacagttat cggcttcatc agctgttgca aaatgggtga
    33661 aaatcccctt gacagaggca ccactttccc ccaattcaga aattagctta tttccttcat
    33721 ctaactggcg aacacctata cgtcccattc cactatcaac tttgatatgt acttctagac
    33781 cagataaatc tactgctgat gtctgacata aacgtaacca ctccaagcta gtaactgtta
    33841 gagtaatatt ttcatttttg gctagtataa cctgctcagg cataacaaca cctaatacca
    33901 atatcatttt tacaattcct gcttgacgta gctcaatagc ctcatctaaa ttggatacgc
    33961 aaaaaccatc tacaatagac tcaatgtatt ttgaaacttc aacagcacca tgcccataag
    34021 cattagcctt aactacagca aatgtttttt tatcacttgg aatgtgctct tgtacagatt
    34081 taacgttatt tgcaatcgcc tctaaatcta tcaaggctct ggttggtcta tgataacttg
    34141 aaatcatttt tcttcctcca aaataacact agcttgtgca taattaccac tatgagatat
    34201 tgaaacaaaa gattttccat taaacggttc ttttgtaatc aaaggcgctc ctttatcatc
    34261 cgataaaatt tcgatatcat gaaaattaac tttcccaatt cctgttccaa gtgctttgct
    34321 ataagcctct tttgctgccc atcgccctgt taaaaaagac atctgacgct tgggattgga
    34381 aattccttta aaaagaagca attcttgttc ggtcaaaacg cgttctgcaa aacgttgatt
    34441 acgctcatat gctttagtaa tcgcctctat ctcttgtaaa tcaattccgt gtccaacaat
    34501 catgtgtaac tcctataagt tcgatactta caaactattg taaatatccc taattagcat
    34561 ttctgtttta tcccaaccta gacatggatc tgtgatagac ttaccaaaaa catctggttt
    34621 atcttgtctt ccatcttcta aataagattc tatcaaaaaa ccacgaacat agtttctaat
    34681 tttagtatgc cactgtcggt ctgcaagtac tgatttcaca atgcgaattt gttctaaaaa
    34741 ttgcttacct gaattgtcat gatttgtatc aacaacaata aagggatttt gcaaatcagt
    34801 ctcttcataa tgagtaatag tttctaaaag gttttcataa tgataattag ggatacttcg
    34861 atagtcggca ttatgatatc ctctcaaaat aacatgtgac agcaaattac catctgttct
    34921 aacttgctta ttttgataga ataattcctg ttgattttgt gctgcataga ccgcattaaa
    34981 catgactctt aaattaccag acgttggatt tttcatacct actggtgcat ctatacctga
    35041 agatataaaa cggtgtccct gatcttccac tgaacgcgca ccaatagcat agtaagaaac
    35101 taaatcatcc atcactgata acatctctgg ataaagtaat tcatcagctg tcaggagtcc
    35161 agtctctcga atgatgtcat agtgcatctt acgtgcttga aaaaaaactc ctagtttgtc
    35221 agaatggtga actaagcctt tataaccatc accattcgtt cttggcttag cggtataaac
    35281 tctcataacc aaaaacattt tatctttaaa ggcagcttcc aatttagcta aacgctttgc
    35341 ataagttagc accgcctttg gattatccgc agaacaagga cctactatta ctagaaaccg
    35401 ctgatcctca ccttttatga tattttttaa ctcttcttct ttggctaatt ttttttggct
    35461 aacttctaga tctaagctag ttttctgctt caatatttca atattaattt tatcacttaa
    35521 ctgatgaaat cccatgtttt ttcctaatac aacctaagtc tattaataag ctctgctata
    35581 caatatagtc gtcatactat tagacaagta atgataaaac agtcttctca gactgtttta
    35641 tttttatttt aaaatcattg ttattgtaaa catctcagac catgacaatt tttaaacttc
    35701 atgccagagc cacaaggaca tttatcatta cgtttaatcc cttggtattc tggtgattca
    35761 ttattcatag gtacatgttg tgccgagata ttttgctctg ctgttgtcgt agcatgttga
    35821 gaagcacgtt cacgctcttg ttcatgaatt tgagctttca tcaatgtacg tgtcacatca
    35881 aactcaatcg aaccaatcat atcttggaac atacggaaac cttctgattg atactcaacg
    35941 attgggttat tttgagcata accacgaagt ccaacggagt tacgtaattg atctaaggca
    36001 tcaatatgct ctgtccattg attatccact accatcaaga ttaagacttt ttggaattcg
    36061 ataatagctt ctggatcttt taatttagca atttgttgct cataaacagc taaggcacgt
    36121 tcataaagaa gttctttaat ttcagcctct tttagaccac gaagttctga aactgtgata
    36181 gcttcttcat ctaataacgc cgatcttgca aaattaacaa tagcttctgc tgcagtattt
    36241 ttatcgcttc tgctatgtgc atcaactgct cttttaatcg tacgtttaat catccctttg
    36301 agctctgatc ctaaatcacg ctcagcagta atcacttctc tacggttagc ataaataatc
    36361 tcacgttgtt cacgcataac atcatcgtat tgcaagactt gtttacgtgt gtcatagtta
    36421 ttaccttcaa cgcggcgttg agctgattca acttgtcgtg tcaacatttt tgatttaata
    36481 actgtatcat cttcagctaa attcattcgc tcaagaacca ctttaatacg atctgtacca
    36541 aagcgacgca tcaaatcatc ttctaacgaa agatagaatt gtgattcacc tggatctcct
    36601 tgacgacccg aacgaccacg aagctggtta tcaatacgac ggctttcatg acgctctgta
    36661 ccaataacac ataaaccacc aagttcacga accccttcac caagcttgat atcagtacca
    36721 cgaccagcca tgttggttgc gattgtaaca gcaccacgtt gcccagcatt cataatgatt
    36781 tgagcttctt taaagtgatt tttagcattc aaaacttcat gtggtactcc agcagcaact
    36841 aattttctag aaattaagtc tgacgtttca acagctactg taccaaccag tacaggttgt
    36901 ccttgttcat aacgttcttt gacatctgct accaccgctc taaatttaga gtcaagagtt
    36961 ggataaagta gatctgaatg atctatacgt tgaacaggac gatttgttgg gataggaata
    37021 acgcgcatat tataaatttc acgaaactct tcttcctcag tcttaccagt accagtcatt
    37081 cctgctaatt tatggtacat acggaacata ttttggtaag taattgaagc gctcgttttt
    37141 gattcttctt ggattggcac tgactctttt gcttcaatag cttggtgtaa gccatctgag
    37201 aaacgacgtc cttccatcgt acgaccggta aattggtcca caattaaaat ctcttgttct
    37261 tcactaacca cataatcaat atttaacaac ataatatagt tagctcgaag agcgttatca
    37321 atgtagtgag ttaaggcaac attttcgaga tcatacaagt tatttagatg gaaatagttt
    37381 tcagccttgt caatacctgt atctgataaa ccaattgttt ttgttggaac atcaatgata
    37441 taatcatcgc tattaagcgt ttttacaaac atgtccgcac gtgtatacag ttggttcatc
    37501 tcagagctta caggacctga aacaatcaat ggcgtcctag cttcatcgat taatacagag
    37561 tcaacctcat caaccaaagc atagttgagt gggcgttgca ccatatcttc ttggcgaaca
    37621 accatattgt ctcgaaggta gtcaaatccg acttctgcgt tagttgagta tgtgatatca
    37681 caattgtaag cttcgcgttt ttcaaatggt gacttagctg ctaagttaat ccctacagat
    37741 aatccaagcc aactataaac ctcacccatt tcggtcgcat cacgtgttga taagtactca
    37801 ttaacagtaa taacatgaac acctaatcct gagatagcat ttagataaac aggcattgtc
    37861 gctgttaaag tttttccctc accagtacgc atttctggaa tatcaccatg gtgaagaacg
    37921 ataccaccca taatttggac gtggtatggg tataaaccta atacacgttt agaagcttca
    37981 cgaactaccg caaacgcttc cggtaacaat tgatctaaag tttcaccatt ttgataacgt
    38041 tctttaaatt ctggagtttt tgcttgtaaa gcttcatctg ataacgtagc catatgatct
    38101 gcgtaagagt caactttctt agctatttta tctaattttt ttaattcacc cttgtcattt
    38161 tcaataacag tgcgtagaat atttgccatt ctaattcctt tcgatactct tatcaccaca
    38221 ttttaccata aataatgttg tctttcaata aatggtcgtt ttaatcgaga ttaaaacaag
    38281 tggtatcccc actctcatta ttttttaaca tgacttgcta taatttctaa ctgtccatca
    38341 atatcccatt tgacaacatc atttggcaag ataaagtggt cacctttttt aagtgtataa
    38401 actttgttat ctactgtaat gtgacccgct ccgtctaaga cactaaccaa aaggtagggt
    38461 gcaaattgtt taaagttggt aacaccagaa atttcccatt tataaactgt aaagaaatca
    38521 tttgaaacta ataaagttga agatagatgt tttaacttca tattggcagg aacagtattg
    38581 gcaggtttac caatcgtgag aacatctatt gattgttcaa tgcgtaaatc acgtaatttt
    38641 cctgaagcat ctggacgatc aaaatcataa acacgataag tagtatcact agattgctgt
    38701 gtttctaaaa ttaatatccc tttacctata gcatgcattg tcccacttgg aacgtagtaa
    38761 aaatcccctg atttaacagg aattcgggtt aaaagatgtt cccaatctcc agattcaatc
    38821 atttggcgaa gttcttcttt agttttagca ttatgaccat agataatttc agatccttca
    38881 tcagcagaaa taatatacca acattctgtt ttaccaagtt caccttcatg tttaagagca
    38941 taagcatcat ccggatgaac ctgtacacta agccagtcat tagcatctaa gattttagtt
    39001 agtaaaggaa agacagtatc atcagggttg ccaaataagc ttttttcacc ttgatacagc
    39061 ttatctaaaa aacaaccttt aaagcgacca ttcttaactc ttgagacacc gttaggatga
    39121 gctgaaatag cccaatattc tcctgttgtt tcacttggaa tatcatagcc aaattcatcc
    39181 cttaactttg ttccacccca gattttatcg tgcatgcttg cttctaaaaa taatggttcc
    39241 gacataagaa tcccctatct tttgtaaaat tcaagcctat tatagcattt tatccatcat
    39301 atcgcttact ttttttaatc ttttatttgc taactaaact aagtagtggc ttaagggttt
    39361 tattaacctt ttgatatttt ctttgctaaa gcaaaatttc ctaacgttgc agaaccattt
    39421 tcttctatag ctggtgtcac aatataatca gacaaatcgg gaactggtaa atacccattt
    39481 aataaagttg caaatgtctg gcgaacacgt cttaacatgt gctcttgtgc catgacgcct
    39541 ccaccaaata caatgacttg tggacgatat aatacagttg cttgtattgc agcctgagca
    39601 atataatagg cttgaacatc ccaaaccatt gaattttctt caatcaattc accacgaata
    39661 ccagtcctag cttctagagt tggaccagat gccaaacctt ctaaacagct attatgaaac
    39721 ggacagatac ctttaaaatc atttgcttgg tcctgcggat gcatcgccat ataagtatga
    39781 cctgcttcag tatgaccagt tcctcctata aattcaccct tttgtatagc tcctgcacca
    39841 attcccgtac caatggtgta ataaacaagt gaatcaatat tgttacgagc tataacttca
    39901 ccataggcag agctattaac atctgttgtg aaataaaatg gaacattgaa atctttagaa
    39961 attaagccga gtaaatcaac attagcccaa tgtaactttg gggtcgttgt gatataaccg
    40021 tatgtctttg attttttatc aatatcaatg ggaccaaaag aaccaatagc aactgcctct
    40081 aacttctttt caaatctttt aaagaaatca acagtctttt taatcgtttc ttgaggcgtc
    40141 gttgttggaa attgcatttt ttctacaact tttaattcct catcaccaac tgcacaaaca
    40201 aactttgtcc cacctgcttc aatactacca tataactttg tcatcttttt tcctatctat
    40261 aatctctatc tcaaagacaa aggttgggat aattatccca acctttaatc ctcacttaac
    40321 atcaataatc atgtcacctg tagtaacaac accctctttt accaatggta taacatcagc
    40381 aaaatcagct gtattagtca caataatcat agctgtatta tccagacctg cttctgcaat
    40441 cttggctgaa tcaaaggtgc caagaatatc accttttttc actttttggt ctgccttgac
    40501 tttagaagta aaccctgtgc cattcatggt gacagtatca atacctatat gaatcaaaat
    40561 ttctgcacct ttatctgatt tgataccgta agcatgacct gtttcaaaag caatttggac
    40621 aaaaccatca acaggtgagt aaattgtatt acctcttggt ttaatagcaa ggccatctcc
    40681 cataatacct gatgaaaaaa ctgggtcact tactgtatca agagcaataa cctgtccatc
    40741 aagagggcta acaatcgttt ctgcccttaa ttggctagtt tgagtaattg ttggatcgtc
    40801 atctgtttct tcaaccaaag gactaacttc agctactgct ttttcatctt tatcttcata
    40861 tccaaacata taagttaata caaaagccaa agacgtagtt ccaattacca taataaggta
    40921 cttaacaatt tgaccattta aataaagtaa tgttcctggg ataatggtaa taccaaaacc
    40981 agttcctgct aatttcaata ttgaagctaa ccagccacct gctgctcctg caattaatcc
    41041 cataataaat ggcttaccaa aacgtaagtt tactccaaat atagccggtt ctgtaatacc
    41101 aagtccagct gaaagcgctg ccggaaaagc aagggctttt aattttttat tttttgtttt
    41161 aacaccaact gccaacgtgg cacctgcttg tgctgtcata gcagctgtaa taatagcatt
    41221 aaatggatct ttcccatcag cagcaattaa ctgagcttca agtaaattaa agatatgatg
    41281 aacacctgta acaacgatta tctgatgaac accacctaaa ataagacctg ataggccaag
    41341 aggtaaatta aggacaaatt ttgtccctgc taaaacatag ttttcaactg aatggaaaat
    41401 aggaccaatg acaaaaagtg ctaaaattga cataactgta aaagttagga acggcactaa
    41461 tagtaaatca agaacatctg ggattttctt atgcaaccat ttttcaagtt tagcaccaag
    41521 taaacctaca aagaatgccg gcaatactga attttgataa ccaactactg gaataaagcc
    41581 gaaaaatttt atgggatggg catcacctga ggccactgcc caagcgtttg gaagggcaga
    41641 gttgaccatc atcaatccta atacgagacc gataacagga tttccaccaa aaactctaaa
    41701 agctgaccaa gaaatcaagg caggaaaaaa agcaaaggcc gtgtcagtca aaacgacagt
    41761 ataggtataa aaattagaac ttgaaaatgc ttttgaagtc gtcccaaata aagctaaaac
    41821 tgtatcatta ttaatcgcac cgcgaatccc cataaataaa ccagtagcaa caatggctgg
    41881 caacaatgga acaaaaacat caccgaaagt acgaatagct cgttggaaag cattcccttg
    41941 tttagccgct tctgcctttt gatcagaggt tgacaaagtt ggcaaacctt gagccactac
    42001 ttcatcataa atcttattaa cagtaccagt tccaaaaata atttgatatt gacctgaatt
    42061 gaaaaatgct ccctgaactt tttcgatatt ttcaacagta tttttatcaa tgactgtctc
    42121 atcttttacc ataatgcgaa gacgagtggc acagtgtgct acgctacgca cattactagc
    42181 accaccaatc gcattaatga cttgtttagc aatttctgta tttgaagcca tctataaatc
    42241 tcctttgaca ttttgaaagc gtttcatttt atttactcta atattctagc atgtctcata
    42301 aatatatgtc aagcgtttga cataaaataa tattttttga tgttaagagt tgatttcaaa
    42361 agagaaaacg tttactatat agttattaga tattaagaca gtcaaggagt atcaaatgaa
    42421 tttacccact gaaatccgct atcgtcctta tgatgaatgg actgaagaag ataaggaaaa
    42481 tattttaaag aatgtctcaa aatccccttg gagagcaact tatcacttag aagctaaaac
    42541 agggctttta aatgacccaa atggcttctc ttactttaat ggaaaattcc atctttttta
    42601 tcaaaattgg ccctttggag ctgcacatgg tttaaaacaa tgggtacata cagaatcaga
    42661 tgatttagtt catttcaaag aaacaggaat caagcttaaa cctgaccatg tcaatgatag
    42721 ccatggtgca tactccggtt ctgcattagc cattgacgat aagctttttc ttttttatac
    42781 tggtaatgtc cgtgacatga aatggaaccg tgacccccgc caaattggag catggatgac
    42841 aagtgatggc aagattacaa aatttgataa agtccttatt tcacaaccaa atgatgtcac
    42901 tgagcatttt cgtgatcctc agattttcaa ttatgataat caattctatg ctgttatcgg
    42961 ggcgcaaaat agtaaaaaat gcggattcat taaattatat aaagcactta ataatgacat
    43021 ccatcattgg gagtttgtgg gagatttaga ttttggtggt actggatctg agtatatgat
    43081 tgaatgtcca aatattattt ttgtcaaagg aaaacctgta ttactttata gtcctcaagg
    43141 actagataaa aacgaattgg actaccaaaa catttatcca aatacctaca aaatcggaca
    43201 atatttcgat gctaactcat ctaagatagt tgaaccatcc cctatctata atttagatta
    43261 tggttttgaa gcctatgcta ctcaaggatt taatacttct gatggtcgta cctttatcgt
    43321 gagttggata ggcttgccag atattgatta tcctagcgat caatttgatt atcaaggagc
    43381 tatgagttta gttaaagagt taagcataaa aaatgggaac ctttatcaat atcccgttcc
    43441 tgctatgaaa aatcttcgtc aacatcaagc agaatttaaa acacaacttc aaactaacaa
    43501 cacctatgaa ttagaattac tagttcctag aaacgatctt tcttcatttg ttctctttgc
    43561 taatccgaaa ggacaaggac tatccattac tattgatact gcaaagggga aagttatcgt
    43621 tgaccgctca caagccggac agcagtacgc cacagaattt ggtactagtc gccagtgtga
    43681 cattccaaaa gatgctacta gtatcaatat ttttatcgat aagtccatct ttgaaatctt
    43741 tataaataaa ggagaaaaag tatttacggg acgagtcttt ccagatgcag aacaatcagg
    43801 aattcaacta aaagaaggac acgttcatgg taaatatttt gaattaaaat attaatatgg
    43861 ttgcaaaatt aacagatgtt gctgcacttg ccggtgttag cccaacgact gtatcacgcg
    43921 ttattaacaa aaaaggctat ttatctcaaa aaacagtcac caaagtcaat gaagccatga
    43981 gaacccttgg ttataaacct aataatttag cacgtagctt acagggaaag tctgctaaat
    44041 taattggact cattttcccc aacatccgta acatttttta cgctgaatta attgaacacc
    44101 ttgaaattga gttgtttaag catggttata aaactatctt atgtaatagt gaaaaggatc
    44161 ctatcaaaga aaaagaatac ctcgaaatgc taggagcaaa tcaagtagac ggtatcattt
    44221 caagtagtca caacctagga attgatgatt acgagaaagt tgaggctccc atagtagcat
    44281 tcgatagaaa tttagctcca cacatcccga tcatctcatc tgataatttc tttggaggaa
    44341 aaatggctgc tcaaaccctt aaaaaacatg gctgtcaaaa gatgattatg attacaggga
    44401 atgataattc agattctcct actggactta ggcggctagg tttttcctat gaatcaaagg
    44461 aatccaaagt tatcacagtc acaaatgggc tatctaacat gagacgtgag atggaattaa
    44521 aatcaattat ctcgactcac aaaccagatg gtatttttac atcagacgac ttaactgctt
    44581 tattagtaat caaacttatc agccaactag ggttgtccat cccagaagat atcaaagtga
    44641 ttggatatga tggtacaagt ttcattcaag attacgtccc acatctaacc actattaaac
    44701 aacctattcg agaaattgcg caattgatgg ttgaaatact cctagctaag attgaggggc
    44761 aaaagacaaa taaggattat attttaccag tcagtctcat ccctggtagt agtgtttagg
    44821 aaatcatatt tataaaataa tttttagtaa tagttatttt tcaaagcaaa ttaaaaatgt
    44881 ggaagaatct tcttccacat ttttaatttt catttgttat aaattggctt aataaaccat
    44941 tgacaaattt tgctgacgtc tcatctgaat attttttagc aatctcgata atctcattta
    45001 gagctacacg atcaggcgtt tcatcaaaat atttaatttc atagaggcct aagcgtaaga
    45061 gagatttatc aaccaatgtt agacgttcta agctccatcc tgattttaag tgactagaaa
    45121 tcaaagtatc taattcatcc ttgtgatcta caacaccatt aacaagatta agtaaaaaga
    45181 ttggaacctc taaaactttg tcttcagata ctgttttatc ataaccataa gcaaagtgag
    45241 ccgcatcaat aaactctcca ccagtttcta agctaaatag tgtttgaaaa gcacgttcac
    45301 gtaaatcacg tcttgaatct ttaaatacac tagtcatcta agaaatcctc atcaaacaat
    45361 gacttcaagt ctggttttgg tgttttctct ggaacaatac cttcaacatg gatattgacc
    45421 gaagaaattt ttacttctgc catgtcatat acagctgttt tgacagcttg ttgaatagca
    45481 attgaaactg ctggtacatt tacaccatac tgcaagtaaa cataaatatc tgcagctact
    45541 gtatcatctt cttcattttt aaggtagaca cctctaccta gagatttctt actaaggcta
    45601 tccgttaccg ctttgttacg taacgaatga acaccatcaa ctttagttgc agcaatacct
    45661 gtaattacct caagtacacg tggtgaaatg acaatttctc caagattttc agttgtcata
    45721 gcttatcctt tcttttagtg tttactaagg tttttcctta gtagagtaaa ttcatcatga
    45781 atctacttaa cacttttttg agagtcatta ggcgcgtgaa acgtaagttc cttctgctgt
    45841 attgattact aacttttgtc cagcttcaat aaagtctgga acgttaacaa caagtcctgt
    45901 ttccatagtt gcaggttttc ctgaaccagt tacagtagca cctttgattg atggttgagt
    45961 ttcagcaact gtcaattcaa cagttgttgg aatttgaaca ccaataactt ctgttccata
    46021 gaattgaatt tttacttctg agttttcaag aatgtagagt aattcatttt caatgttaac
    46081 agttggaatt tcatattgat cgtaagtttc attattcatg aagtaagctg tatcatccat
    46141 cttataaagg tattgagcag gtacagtttc aataatagct tgctcaaatt tttcttcagg
    46201 acggtaactt gtatcaaaag ttgaaccagt acgaacatca cgcaatttca tacgcatgat
    46261 tgtgttacct ttacctggtt tgtggtggct agcttcaaga acacggatta atttcccatc
    46321 agccgtttca aaagtcatgc ctgctttaag cttacttgct tcaatcattt attaatacct
    46381 ctttgtaaaa attatttatc aactaatttt atcacaaata acctgctttc tcaaatagtt
    46441 tattgatttg gtatgtgtct gatttcttga taaataaata aaatcaccaa gagggcgtaa
    46501 ataagactga agcacttgac actttcctct tacttcagtt ttgataacag ttttaatatt
    46561 ataccgatat cttgactaag attgaaatac tgtttaggtg tttttagtga ctttttagta
    46621 aattttaata aaggtagtaa actatagttg tcttattgaa acagataaaa taaatcacct
    46681 acggagctat tcgtatgaaa atgtagttat tctcgatata agttttagat acgaacttaa
    46741 tttgctctat tgaagctggt aaaggaaatc ggggtggtgt tagtggacgt gcgctaagca
    46801 ttggtactga gcttggtgat accaatcttg cttactacat caggtagctc tctgttttag
    46861 tgccatatgc aattggaata agagctgtca ttggtattga aggagcccta gtccactgat
    46921 cattgaatta ctaatttagg aggtggaatg atgaatttta atatgtctca ttatagaaag
    46981 ttacaactat gtcgttttct aatcgtacag atacctgcgc tggctttatt agctattctt
    47041 ttatacggga ttttcctaaa gtacagtcta ttttcaaaag tattttttct tatcttttag
    47101 gggttgaaat agttatcaat tttgggaagt acataccagt ttacaccaac cacaatttgt
    47161 cgagtaaaga attgtaattt agtaaagcct ttttcgtttt cttctgataa gtgtatcatg
    47221 acatctatca atagcatatt cacactagta gcatattgat ttgtttttga gtagaattta
    47281 ctttttagat tatttctatt ctacactagt ctttcccttt ggaagattct ttccctctgt
    47341 atttttccat ttttcaatga gacgagaaac tctttcttcc tcctcatcat ggtaaccttc
    47401 aaaaacaacc tcatcaatat cttcttgatt aaaatagtaa acttgttcaa gatttaaacc
    47461 ctcaggaaaa attccaccta ggtaatcaaa ataaacttct tgtccttctt gctcaacaat
    47521 tgcacctcta tttacaataa ctaatttttg attgccatca ataagataga caacactccc
    47581 tactggtaat aacatacttc agttctcccc aatataataa tttttcccat ttaacgatgg
    47641 ttgaaactca atgaacttta taaaaaataa actgtttgaa aaaataaatc caaataaaaa
    47701 taagtttgaa aagttttcgt cccacaatgc cattcccc
//
LOCUS       DATRUK010000010        44032 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.010, whole genome shotgun sequence.
ACCESSION   DATRUK010000010 DATRUK010000000
VERSION     DATRUK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44032
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.010"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            156..812
                     /locus_tag="VBE34_000484"
     CDS             156..812
                     /locus_tag="VBE34_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7263226.1"
                     /translation="MSYLTFKHVDFKTDDKLVLNDINFAIDEGDFVSIVGPSGSGKST
                     VLKLASGLMSPTAGHIFFDGKDLNQLEPIESRKMISYCFQTPHLFGNTVEDNISFPYH
                     IRHEKVDYRRVDDLFQRFEMDQSYLKQDVKKLSGGEKQRIALIRQLLFEPKVLLLDEV
                     TSALDNHNKAIVEKVIKSLHDKGITILWITHDEEQSRRFANKVLKVVNGSIEAMEVIK
                     "
     gene            809..1582
                     /gene="fetB"
                     /locus_tag="VBE34_000485"
     CDS             809..1582
                     /gene="fetB"
                     /locus_tag="VBE34_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEO7263227.1"
                     /translation="MSGANSISLMSLLMASSLVLITLFFSYWQKLNLEKEVIISAIRA
                     VIQLLAVGFLLDYIFGYQNPIFTALLMLFMIINASYNAAKRGKGINKGFVISFIAIGS
                     GTIITLSVLIFSGILKFVPNQMIPVGGMIISNSMVAIGLCYKQLLSEFRSKQEEVETK
                     LALGADILPASIDIIRDVIKTGMVPTIDSAKTLGIVSLPGMMTGLILAGTSPIQAVKY
                     QMMVTFMLLATTSIASFVATYLAYKIFFNNRKQLVVTKD"
     gene            1746..2564
                     /locus_tag="VBE34_000486"
     CDS             1746..2564
                     /locus_tag="VBE34_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEO7263228.1"
                     /translation="MILKICRAAYSLQWGGVYQLALLDYPRIKAFELERIGAFIAYEK
                     QYKRKTEIQCDDKHLLAKIVHFLKYNSFTFPYIPKYREAAATFNEDGISLTSDFLSHT
                     CTIETAKLIFKEGKILSAVKAFNKPAEVLVKDKRNAAGDPKDYFDYVMLNWSNTNSGY
                     RLVMERLLGKAPSEQELTVGFKPGVSFHFTYQDIINHPDSIFDGYHPAKIKNQLSLAE
                     HLVACVIPKHYQEDYQSLVPNDLKHRVYYLDYCNETLYEWNQKVYDFLCHLENK"
     gene            complement(2601..3485)
                     /locus_tag="VBE34_000487"
     CDS             complement(2601..3485)
                     /locus_tag="VBE34_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7263229.1"
                     /translation="MDIRQLTYFIAVAEAKNYSRAAKSLFVTQPTLSQSIKKLEAELN
                     TILFLQNGRQLALTEAGEILYEKGQLLMTNVNQMVTEIQQLNQEKKEGVRVGLTSLFA
                     IQFMKQISTFMATHSNVEVSLIQDGSRKLQELLAKGKIDIGLLSFPSTRNDITIEPLQ
                     TSTKGYKVSIVMPKSHPLATLPEIELNDLRDYKVASLNEHYMLGEMLPRKCRALGFDP
                     HIVFKHNDWEVLIHSLQDLNAVTILPSEFESISQVQDLCWVPLKDKNNFYPIGIAYRN
                     DTSFSPMIEEFLSLLKTN"
     gene            3678..4736
                     /locus_tag="VBE34_000488"
     CDS             3678..4736
                     /locus_tag="VBE34_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO7263230.1"
                     /translation="MSDVVEKQTTKSFIMNVLNGLALGTVIVLIPGAILGELMKALLP
                     MWSGFATLIAATAVATSMMGLVIGIMVGLNFKFNPIQSASLGLAVMFAGGAATFLKGA
                     IMLKGTGDIINMGITAALGVLLIQFLSDKTKSFTLIVIPTVTLLLVGGVGHVLLPYVK
                     MITTMIGQGIASLLGLQPVLMSILIAMIFCFLIVSPITTVGIALAISLSGIGSGAANL
                     GICAASFGLCMAGWSVNSKGTALAHVLGSPKISMANVLAKPKIMLPMISSAAILGILG
                     ALFNIQGTPASAGFGISGLIGPINALNLAKGGWSVMNMLLIIIIFVAAPIILNFIFNY
                     LFIKVLKIIDPMDYKLDI"
     gene            4750..5742
                     /locus_tag="VBE34_000489"
     CDS             4750..5742
                     /locus_tag="VBE34_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEO7263231.1"
                     /translation="MKLKVFNVREEEATLAQDWANRNHVELSMSEGPLTLETVNEVEG
                     FDGIANAQIEPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLELAKQHGIIISNVPSYSP
                     ESIAEFTVTIALNLIRKVELIRANVREQNFSWTLPIRGRVLGNMTVAIIGTGRIGLAT
                     AKIFKGFGCRVIGYDIYHNPMADGILEYVNSVEEAVEEADLVSLHMPPTAENTHLFNL
                     DMFKQFKKGAILMNMARGALVETKDLLEALDQGLLEGAGIDTYEFEGPYIPKNCQGQD
                     ISDKDFLRLINHPKVIYTPHAAYYTDEAVKNLVEGALNACVEVIETGTTTTKVN"
     gene            5949..7499
                     /locus_tag="VBE34_000490"
     CDS             5949..7499
                     /locus_tag="VBE34_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEO7263232.1"
                     /translation="MEKTMDMEQREEVVSYNRAKVWQLVMFAMNNASTNIYLFTFMFV
                     TYFSTGVLGSAAIFVSQIMGYIRIFDGFIDPAIGIMIDKTDTKFGKYRPILIIGNVIT
                     ALSLIFLLALRGVDENIRFPLFILVLIIHKIGYSMQQTITKAGQTALTNDPKQRPIFN
                     IVDAVMTTSLMTGGQFVVSVFLVPKFGNFTPQFFNVLIFGTILISAILAIVAIIGIWA
                     KDRKEFFGLGENTQKTALKDYWKVLKGNKPLQILSIAAALVKFAIQFFGDSVVMVLLF
                     GILFGNYALSGQFSLLFIVPGVIINILFSTIARKKGLRFSYVRAIQIGMIGLLAFGAV
                     LYVGKPGDLSLTSLNLYTILFIVTNIIARYASQAPASLVLTMGADISDYETSESGRYV
                     SGMIGTIFSLTDSIASSFAPMVVGFVLAGIGFSKSFPTIETPLPPDLKMAAISILVAI
                     PFIALSIALLLMKFYKLDKEEMVRIQEKIQVMKAATTKERAKDIARNVPLTDMDYVDV
                     TKYPIDKD"
     gene            7566..8591
                     /locus_tag="VBE34_000491"
     CDS             7566..8591
                     /locus_tag="VBE34_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO7263233.1"
                     /translation="MAKIISLGEVLLRLSPPQYHTLMQANHLKCQFGGSELNVLASLA
                     QLGYHVGLVSALPDNDLGKMASQFILSQQISPAAIIKKEGRLGIYYYEQGFSVRTNKV
                     IYDRNYSSFWESTLSDYDFTSIFKGVDWFHVSGITPALTKDLYEVTRFLMTKAKEGGV
                     KVSIDLNFRESLWSSFQEAREQLSPLLGLLDVCFGLEPIYLAGESEDLKDELGLSRPY
                     LDIELLEKITQKIVQEYGLDYIAFTQREMEYTNQYMLKSYLYHNNMLYQTDKTGVEVL
                     DRVGTGDAFAAGLIHALLEKETPQRALEIAMATFKYKHTIQGDINIMTRDDIAYLIEK
                     ETNDIKR"
     gene            8608..10407
                     /gene="uidA"
                     /locus_tag="VBE34_000492"
     CDS             8608..10407
                     /gene="uidA"
                     /locus_tag="VBE34_000492"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEO7263234.1"
                     /translation="MLYPLLTKTRNTYDLGGIWNFKLGEHNPNELLPSDEVMVIPTSF
                     NDLMVSKEKRDYIGDFWYEKVIEVPKVSEDEEMVLRFGSVTHQAKIYVDGVLVGEHKG
                     GFTPFEVLVPECKYNNEKIKVSICANNVLDYTTLPVGNYSEIIQEDGSIKKKVRENFD
                     FFNYAGVHRPLKLMIRPKNHIFDITITSRLSDDLQSADLHFLVETNQKVDEVRISVFD
                     EDNKLVGETKDSRLFLSDVHLWEVLNAYLYTARVEIFVDNQLQDVYEENFGLREIEVT
                     NGQFLLNRKPIYFKGFGKHEDTFINGRGLNEAANLMDLNLLKDMGANSFRTSHYPYSE
                     EMMRLADRMGVLVIDEVPAVGLFQNFNASLDLSPKDNGTWNLMQTKAAHEQAIQELVK
                     RDKNHPSVVMWVVANEPASHEAGAHDYFEPLVKLYKDLDPQKRPVTLVNILMATPDRD
                     QVMDLVDVVCLNRYYGWYVDHGDLTNAEVGIRKELLEWQDKFPDKPIIITEYGADTLP
                     GLHSTWNIPYTEEFQCDFYEMSHRVFDGIPNLVGEQVWNFADFETNLMILRVQGNHKG
                     LFSRNRQPKQVVKEFKKRWMTIPHYHNKKNSVK"
     gene            10436..11107
                     /locus_tag="VBE34_000493"
     CDS             10436..11107
                     /locus_tag="VBE34_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEO7263235.1"
                     /translation="MARPLVEQTADRLLHLILEREYPVGAKLPNEYELAEDLDVGRST
                     IREAVRSLATRNILEVRQGSGTYISSKKGVSEDPLGFSLIKDTDRLTSDLFELRLLLE
                     PRIAELVAYRITDDQLQLLEKLVGDIEDAVHAGDPKHLLLDVEFHSMLAKYSGNIAMD
                     SLLPVINQSIHLINANYTNRQMKSDSLEAHREIIKAIREKNPVAAHDAMLMHIMSVRR
                     SALKA"
     gene            11224..11845
                     /gene="eda"
                     /locus_tag="VBE34_000494"
                     /pseudo
     CDS             11224..11845
                     /gene="eda"
                     /locus_tag="VBE34_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771912.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
     gene            11862..13262
                     /gene="uxaC"
                     /locus_tag="VBE34_000495"
     CDS             11862..13262
                     /gene="uxaC"
                     /locus_tag="VBE34_000495"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEO7263236.1"
                     /translation="MAFNTETFMLKNQAAIQLYEEVKRQPIFDYHCHLDPKDIFEDHI
                     FDNIVDLWLGGDHYKWRLMRANGISEAEITGPASNLEKFKAFARTLERAYGNPVYHWS
                     AMELKNIFGVNEILTESNAEEIYHRLNHFLKEHKISPRRLIADSKVMFIGTTDHPLDT
                     LEWHKKLAADESFKTVVAPTFRPDEAFIEHRHFVDFITKLGDITQKEITDFSTFIAAM
                     EERIAYFAQNGCRASDISFTEIVFEQTDELELNDLFNKVCEGYIPNQSEISKWQTAVF
                     MELCRLYKKYGFVTQVHFGALRNNHSTIFEKLGADVGVDSLGDQVALTVNMNRLLDSL
                     VKKDSLPKMIWYNLNPAYNIAVANTLANFQANELGVRSYLQFGAGWWFADTKLGMISQ
                     MNALAEQGMLANFIGMLTDSRSFLSYQRHDYFRRILCTYLGEWIEEGEVPEDYQALGS
                     MAKDIAYQNAVNYFKN"
     gene            13280..14326
                     /gene="uxuA"
                     /locus_tag="VBE34_000496"
     CDS             13280..14326
                     /gene="uxuA"
                     /locus_tag="VBE34_000496"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEO7263237.1"
                     /translation="MEMSFRWYGEDDPVTLENIGQIPTMKGIVTAIYDVPVGEVWSRE
                     RIQQLKEKVEAAGLKISVIESVPVHEDIKLGRPTRDLLIDNYIQTVKNLAAEGIDTIC
                     YNFMPVFDWTRTDLAYQYPDGSTALIFDETVSKKMDPVNGELSLPGWDASYSKEEMKA
                     IMDAYAEIDEEKLWENLTYFIKRIIPEAEAVGVKMAIHPDDPPYSIFGLPRIITGLEA
                     IERFVKLYDSKSNGITLCVGSYASDPQNDVLEISRRAFELDRVNFVHARNIKLGDGKS
                     FKESAHPSEYGSIDMYEVIKLCHEFGFEGAIRPDHGRMIWGETGRPGYGLYDRALGAT
                     YVSGLYEAVIKGSK"
     gene            14450..15289
                     /locus_tag="VBE34_000497"
     CDS             14450..15289
                     /locus_tag="VBE34_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO7263238.1"
                     /translation="MSKTITFTNKVVVITGAGGVLCGYMAKEFAKAGAKVALLDLNQE
                     AAQTFADEIVEEGGIAKAYKANVLSKENLEEVHQAVLEDLGPTDILVNGAGGNNPKAT
                     TDNEFHELDLPSETKTFFELDEAGISFVFNLNYLGTLLPTQVFAQDMVGREGANIINI
                     SSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVGIRCNAIAPGFLVTNQNRSLLF
                     TEDGQPTARAEKILNNTPMGRFGEASELIGGLFFLADEKSSSFVNGVVLPIDGGFAAY
                     SGV"
     gene            15442..16254
                     /locus_tag="VBE34_000498"
     CDS             15442..16254
                     /locus_tag="VBE34_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7263239.1"
                     /translation="MVDTNHKIAFCIDSDGCAMDTMTYKHQLFFGPIAADIFGISEQE
                     RTSFLKEWDRVNLYSRTRGVNRFVGLVMGLEFAGVEHIDKLKEWVETTDSLSNNSLEL
                     ALANDFSRDLEKALEWSNEVNRQIKNYEGDSLAFSGVLESLERLSQFGNVYVVSSANK
                     EAVWQEWEEQGLLKYVDDIYCQDRGKKEDVIATLIASGYCRETILMVGDSLGDLAAAE
                     QNGIAFYPVLVGKEVKSWEILREDIGEAFAKGQFEQQRQKESINTFWANLDN"
     gene            16270..18060
                     /locus_tag="VBE34_000499"
     CDS             16270..18060
                     /locus_tag="VBE34_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEO7263240.1"
                     /translation="MTHLVDLTKKPFNLNQEAIEWIEKTINEMTLDEKIGQLFFNMGA
                     SRSEEYLTDVLDRYHIAAVRYNRGSSSEIYDQNLILQTKSKLPMLIAANTEAGGDGAV
                     TDGTKVGDEIKVAATNDPKYAYEMGRIAGMEASAVGCNASFSPIVDLTRNWRNPIIAS
                     RNWGANVDQIISLSKEYMKGIMQYNIVPFAKHFPGDGIDERDHHLSFASNPMSKEEWM
                     STFGRIYGELADAGLPGVMAGHIHLPNVEKEMHPERDLDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMVGMTASMPRRDLLPTAIEAGCDLFLFFNDPDEDIQWMKEGYEKG
                     ILTEERLHDALRRTLGLKAKLGLHNYEGRRQELFMPKDKAMALINTLESQKIADEVAD
                     KAVTLVKDKQKDIFPVNPERYRHILLVNVEGYKGGFGAMIAGNKQRASDYMKELLEAR
                     GHEVTVWESTEERIMKLPQEERAAAIANVYAQKQPIANLTEHYDLIINLVDVNAGGTT
                     QRIIWPAAKGTPDQPFYVHEIPSIVISVQHAFALADMPQVGTYINAYDGLPSTISAVV
                     AKLAGESEFTGVSPVDAYCGLIDTHLWRQC"
     gene            complement(18118..19203)
                     /locus_tag="VBE34_000500"
     CDS             complement(18118..19203)
                     /locus_tag="VBE34_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO7263241.1"
                     /translation="MSKLNRIRHHLHSVQAELAVFSDPVTVNYLTGFFCDPHERQMFL
                     FVYEDRDPILFVPALEVSRAKQSVPFPVFGYIDSENPWQKIASNLPSFSVSKVLAEFD
                     NLNVTKFQGLQTVFDGHFENLTPYIQNMRLIKSRDEIEKMLVAGEFADKAVQVGFDNI
                     SLNNTETDIIAQIEFEMKKQGINKMSFDTMVLTGNNAANPHGIPGTNKIENNALLLFD
                     LGVETLGYTSDMTRTVAVGKPDQFKKDIYHLCLEAHQAAIDFIKPGVLASEVDAAARN
                     VIEKAGYGQYFNHRLGHGLGMDVHEFPSIMAGNDMEIQEGMCFSVEPGIYIPDKVGVR
                     IEDCGYVTKTGFEVFTKTPKELLYFEG"
     gene            19413..20417
                     /gene="ccpA"
                     /locus_tag="VBE34_000501"
     CDS             19413..20417
                     /gene="ccpA"
                     /locus_tag="VBE34_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEO7263242.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILARGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPIVLAGTVDLEHQLPS
                     VNIDYKAAAVDVIDILAGNHKDIAFVSGPLIDDINGKVRLAGYKEGLKKNGLNFKEGL
                     VFEANYRYAEGFALAQRVINAGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPIAQYTRPNLTSISQPVYDLGAVSMRMLTKIMHKEELEEKEIVLNHGIVKRGTTNN
                     "
     gene            20549..22015
                     /locus_tag="VBE34_000502"
     CDS             20549..22015
                     /locus_tag="VBE34_000502"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEO7263243.1"
                     /translation="MTNELIMQAFEWYLPSDGNHWKKLEESISDLKKLGISKIWLPPA
                     FKGTSSDDVGYGVYDLFDLGEFDQNGTIRTKYGRKEEYLKLIKSLKANGIKPFADIVL
                     NHKANGDHKEKFQVIKVNPENRQEALSEPYEIEGWTGFDFPGRQGEYNDFKWHWYHFT
                     GLDYDAKNNETDIFMIVGDNKGWADDDLIDDENGNFDYLMYNDIDFKHPEVIKNLQDW
                     AKWFIETTGIEGFRLDAVKHIDSYFIQTFINDIRTKIKPDLEVFGEYWKSDQTSMKDY
                     LEATQFQFSLVDVTLHMNFFDASHQNRDFDMRTIFDDSLVIDNPEYAVTFVENHDTQS
                     GQALESRVEDWFKPLAYGLILLRQQGTPCLFYGDYYGIQGEFGQPSFKEVIDKMAELR
                     QNYVFGEQVDYFTHSNCIGWTCLGDEEHNSCLAVVLTNGDQGWKHMEVGEIYAGKTFV
                     DYLGNCEQEVVIGDDGWGDFLVASASISAWVPKIEEAN"
     gene            22060..23058
                     /locus_tag="VBE34_000503"
     CDS             22060..23058
                     /locus_tag="VBE34_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7263244.1"
                     /translation="MKVLLYLEAEEYLKKSGIGRAIKHQEKALQIAGIDYTTNPTDDF
                     DLVHMNTYGIRSWLLMSKAKKTGKKVIMHGHSTEEDFRNSFIGSNLVSPLFKWYLCRF
                     YQKADAIITPTDYSKQLIKAYGIKKPIFVLSNGIDLSRYQRSEKKEAAFRHYFHLSKD
                     DKVVMGAGLYFMRKGIDQFVEVAAKMPDIRFIWFGETNKWVIPRKVRQIVTKQHPSNV
                     TFAGYIKGDVYEGAMSASDAFFFPSREETEGIVVLEALASHQHVVLRDIPVYHGWVTE
                     DSVELATDVDGFVEKLDKVLSGKSDKIKEGYRVAESRSIERIAHELASVYQKVMEL"
     gene            23060..24394
                     /locus_tag="VBE34_000504"
     CDS             23060..24394
                     /locus_tag="VBE34_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO7263245.1"
                     /translation="MRIGLFTDTYFPQVSGVSTSIRTLKEGLEKEGHEVYIFTTTDRN
                     VKRFEDPTIIRLPSVPFISFTDRRVVYRGLISAYRIAKDYELDIIHTQTEFSLGLLGK
                     LVAKALRIPVVHTYHTQYEDYVGYIAKGKLIKPSMVKYIMRTYLSDLDGVICPSRIVL
                     NLLDGYGVKIPKQVIPTGIPVENYRREDISEETIKNLRTELGLADNDTMLLSLSRVSF
                     EKNIQAILMHLSAVVDENPHVKLVIVGDGPYLSDLKELVHSLELENSVIFTGMVEHSQ
                     VAIYYKACDFFISASTSETQGLTYIESLASGRPIIAQSNPYLDDVISDKMFGTLYKKE
                     SDLADAILDAIAETPKMTQEAYEQKLYEISAENFSKSVYAFYLDFLISQKASVKEKVS
                     LTIGNKDSHSTLRFVRKAVYLPKKVFTFTGRASKKVVKAPKRRISSIRDFLD"
     gene            24851..26794
                     /gene="thrS"
                     /locus_tag="VBE34_000505"
     CDS             24851..26794
                     /gene="thrS"
                     /locus_tag="VBE34_000505"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEO7263246.1"
                     /translation="MIKITFPDGAIREFESGITTFEIAQSISNSLAKKALAGKFNGQL
                     IDTTRAIEEDGSIEIVTPDHEDALGVLRHSAAHLFAQAAKRLFPDLCLGVGPAIHDGF
                     YYDTDNKSGQISNDDLPRIEEEMKKIVKENHPCIREEISKEEALELFKDDPYKVELIS
                     EHAEDGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNAMMQRVYGT
                     AWFDKKDLKAYLKRREEAKERDHRKLGKELDLFMVNPEVGQGLPFWLPNGATIRRELE
                     RYIVDKEIASGYQHVYTPPMASVEFYKTSGHWDHYREDMFPTMDMGDGEEFVLRPMNC
                     PHHIEVYKHHVHSYRELPIRIAELGMMHRYEKSGALTGLQRVREMTLNDAHIFVTPEQ
                     IKDEFLKALNLIAEIYEDFNLTDYRFRLSYRDPEDKHKYYDNDEMWENAQAMLKEAMD
                     DFGLDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPIMIHRGGISTMERFTAILIETYKGAFPTWLAPQQVSVIPISNEAHIDYAWEVARA
                     LKDRGIRAEVDDRNEKMQYKIRAAQTQKIPYQLIVGDKEMEEKAVNVRRYGSKATETK
                     SIEEFVESILADIARKSRPDEVK"
     gene            27015..27719
                     /locus_tag="VBE34_000506"
     CDS             27015..27719
                     /locus_tag="VBE34_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7263247.1"
                     /translation="MRTVLVVQGDDETIELLRSYLEGALYKVVMASDGEEAFSLFQQH
                     QIDLAIIDITLPKIDGYELTRLIRQDSQIPIIMLAAKTTDMDRILGLNIGADDFITKP
                     FNSLEVLARINSQLRRYYEFNSLAKPKNQFIKIGELELDEEHVELTKNGKHIKLTATE
                     FKILHILMSSPGKIYTKTQLYEKINGRYLEGDETTIMVHISNIRDKIEDDSKYPKYIK
                     TLRGVGYKIEKPQYLV"
     gene            27817..28836
                     /locus_tag="VBE34_000507"
     CDS             27817..28836
                     /locus_tag="VBE34_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEO7263248.1"
                     /translation="MAKTALNIPFTVEGIENYYQNIKMILSLADKWSIPKDDLLLFLK
                     GLKAIDDIKVGSPIFKYFWTSLSLQAPLKALEFVLEQAKMPTELSGELSETQYLVAQF
                     SDELAPHDDFWIALSQVIYDSFPGNSLAEDTVLNRKLHQFRYLISSQQAQYVRRYFKD
                     VGMTDRDALVNYLSCLREPDSIAYYESARLHNKRRRNGEIFGFPDDEPVINSKLLISF
                     HTEFIIDDKGNFLNEIDAEVITRNGIINGASFNYAFKNNTRHKELDVDPVKLDPKFRN
                     DMTRGYRSPNLSRRKWFFFKEEDYDCSYFNKKGYYAFGRRSAKQSVDKQVKYLKKAVQ
                     KMRIN"
     gene            29003..29569
                     /locus_tag="VBE34_000508"
     CDS             29003..29569
                     /locus_tag="VBE34_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO7263249.1"
                     /translation="MIKKNKVFLGVLLVLVVILGGGVLFYQSQFQKTTNQALAIAYKD
                     AKVAKKDVIHQKIDKEFENFRGSYEIEFNTKSAEYSYHVDAKTGQILERDMDNNGFSK
                     STSQSSSSSSQKSHKISQEEAKKIAFKDANIEESEVSNLKIKEEIENGKSVYDIDFVD
                     LKNKNEVDYQIDAETGKIIERSRDHMND"
     gene            complement(29618..30268)
                     /locus_tag="VBE34_000509"
     CDS             complement(29618..30268)
                     /locus_tag="VBE34_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7263250.1"
                     /translation="MSSVLTLTNILFLLQGFGLTLYISFISILLSMFFGTLLAIMRNS
                     KNPIWKLIASIYIEFVRNVPNLLWIFIIFLVFQMKSVSAGITSFTIFTSAALAEIIRG
                     GLNGVDKGQTEAGLSQGFTYLQVFIIIIFPQAFRKMLPAIISQFVTVIKDTSLLYSVI
                     AIQEIFGKSQILMGRYFEAGQVFTLYAIITAVYFITNFIISSFSRKLSKRWEQATE"
     gene            complement(30280..30975)
                     /locus_tag="VBE34_000510"
     CDS             complement(30280..30975)
                     /locus_tag="VBE34_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7263251.1"
                     /translation="MTLLNISPFAISRWGAFFNHFDLFFKGFLYTLGISFGALLLALI
                     LGILSGGLSTSKSKVGKLISRIYVEVFQNTPLLVQMVFVYYGLAIISNGHIMISAFFT
                     AVLCVGLYHGAYISEVIRSGIEAVPKGQTEAALAQGFTANQTMQLIILPQAIRTILPP
                     MTNQVVNLIKNTSTVAIISGADIMFLAKAWAYDTTNYIPAFAGAAIFYFVICFPLASW
                     ARKQEELNKKTYS"
     gene            complement(30988..31788)
                     /locus_tag="VBE34_000511"
     CDS             complement(30988..31788)
                     /locus_tag="VBE34_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7263252.1"
                     /translation="MLRRKRLTLYLLSCIFIFLLFYPNSTSANQLSEIKKSGVLKVGV
                     KQDVPNFGYYNAETNQYEGMEIDIAKKIAKSLGVKPVFVPTTAQTREPLMDNGQIDIL
                     IATYTITPERKANYNISKAYYHDEIGFLVRKNSHIKTIKELDGKHIGVAQGATTKVNL
                     EKYAKEHKLKFSYAQLGSFPELAISLYANRIDAFSVDKSILSGYLSPHTTILKEGFNT
                     QEYGIATSKQDKVLIPYVNKLLVSWEKDGSLKHIYQKFKLKPAKVKKE"
     gene            complement(31800..32555)
                     /locus_tag="VBE34_000512"
     CDS             complement(31800..32555)
                     /locus_tag="VBE34_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7263253.1"
                     /translation="MSLISYKNVNKYYGDYHALRQINLEIEPGQVVVLLGPSGSGKST
                     LIRTMNALESIDDGSLVVNGHELANISSKELVNLRKEVGMVFQHFNLYPHKTVLENIT
                     LAPIKVLKQSKKEAMEIAEKYLKFVNMWERKDSYPSMLSGGQKQRIAIARGLAMHPKL
                     LLFDEPTSALDPETIGDVLSVMQKLANDGMNMVVVTHEMGFAREVADRIIFMADGEIL
                     VDTTDVQDFFDNPTEPRAKQFLRNIINHTSTDL"
     gene            32895..33605
                     /locus_tag="VBE34_000513"
     CDS             32895..33605
                     /locus_tag="VBE34_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7263254.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYETATAFDGREALVQYAEF
                     QPDLIILDLMLPELDGLEVAKEVRKTSHIPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTENIETAVAEESAQNASSDITIGELQILPDAFIAKKRGEEIE
                     LTHREFELLHHLATHIGQVMTREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPGR
                     PEYILTRRGVGYYMKSYE"
     gene            33598..34947
                     /gene="vicK"
                     /locus_tag="VBE34_000514"
     CDS             33598..34947
                     /gene="vicK"
                     /locus_tag="VBE34_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEO7263255.1"
                     /translation="MNNSAANIRSFELALLFLLVFVAVYFVYLAVRDFKMSKDIRLLN
                     WKVRDLIAGNYSDSILIQGDADLVELGESLNDLSDVFRMAHDNLEQEKNRLASILTYM
                     TDGVLATDRSGKIVMINETAQQQFNLAYDEALSMNIVDMLGSGSPYSFQDLVSKTPEV
                     VLNRRDENGEFVTLRIRFALNRRESGFISGLVAVSHDATEQEKEERERRLFVSNVSHE
                     LRTPLTSVKSYLEALDEGALNEEVAPSFIKVSLDETNRMMRMISDLLSLSRIDNEVTH
                     LDVEMTNFTAFMTSILNRFDQIRNQKTVTGKVYEIVRDYPLKSIWVEIDTDKMTQVID
                     NILNNAVKYSPDGGKITVNLRTTKTQMILSISDQGLGIPKKDLPLIFDRFYRVDKARS
                     RQQGGTGLGLSIAKEIVKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVTYEEWEDVED"
     gene            34951..35760
                     /locus_tag="VBE34_000515"
     CDS             34951..35760
                     /locus_tag="VBE34_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO7263256.1"
                     /translation="MSEIGFKYSILASGSTGNCFYIETPQKRLLIDAGLTGKKVTSLL
                     AEINRKPEDLDAILVTHEHSDHIKGVGVLARKYHLDIYANEQTWKVMDERNMLGKVDV
                     SQKHVFGRGKTLTFGDLDIESFGVSHDAVDPQFYRMMKDDKSFVMLTDTGYVSDRMAS
                     LIENADGYLIESNHDIEILRSGSYPWTLKQRILSDKGHLSNEDGSETMIRTIGNRTKH
                     IYLGHLSKENNIKELAHMTMENNLMRADFGVGTDFSVHDTSPDSATPLTRI"
     gene            35763..36131
                     /locus_tag="VBE34_000516"
     CDS             35763..36131
                     /locus_tag="VBE34_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEO7263257.1"
                     /translation="MKKIFYISLGFISLGIGIAGIVLPVVPTTPLVLLSAFCFSRSSE
                     KFDIWLRQTKVYKYYAADFVESRSIAPARKKSMIWQIYILMGISIYFAPLMWLKLGLL
                     IGTIVGTYVLFYVVPDKIDE"
     gene            36306..36992
                     /locus_tag="VBE34_000517"
     CDS             36306..36992
                     /locus_tag="VBE34_000517"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEO7263258.1"
                     /translation="MKELRSKLEKDYGIVFANQELLDTAFTHTSYANEHRLLNISHNE
                     RLEFLGDAVLQLLISQYLFTKYPQKAEGDLSKLRSMIVREESLAGFSRLCGFDHYIKL
                     GKGEEKSGGRNRDTILGDLFEAFLGALLLDKGVEVVHAFVNKVMIPHVEKGTYERVKD
                     YKTSLQELLQSHGDVKIDYQVTNESGPAHAKEFEVTVSVNQENLSQGIGRSKKAAEQD
                     AAKNALATLQ"
     gene            37000..40539
                     /gene="smc"
                     /locus_tag="VBE34_000518"
     CDS             37000..40539
                     /gene="smc"
                     /locus_tag="VBE34_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEO7263259.1"
                     /translation="MFLKEIEMQGFKSFADKTKVEFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSAKSLRGGKMPDVIFAGTENRKPLNYAQVSVTLDNSDHFIENIADEVRVER
                     RIFRNGDSEYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEAIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQSKLEQTQGNLDRLEDIIYELDMQVQPLEKQASIAKRFLVL
                     DEERQGLHLSILIEDILQHQSDLTTVEEKLLTVREELATYYQQRQSLEDENQSLKQKR
                     HHLSEEIEVKQLALLDVTKLKSDLERQIDLIRLESSQKAEKKEEAGQRLAELEVKAKD
                     CNDQITQKNIELTTLSEKIAQIRSEIVSIESSLERFSTNPDQVIEKLREDFVTLMQEE
                     ADTSNALTALLADIENQKQASQAKSQEIQEVSKNLEVLKSNAKVALERFEAAKKNVRQ
                     LLSHYQDLGQTLQNLEGEYKNQQSILFNHLDEIKSKQARISSLESILKNHSNFYAGVK
                     SVLQAKDQLGGIIGAVSEHLSFDKHYQTALEIALGGSSQHIIVEDESAAKRSIAFLKK
                     NRQGRATFLPLTTIKPRELAQHYLSKLQSSQGFLGIASELVTYDQRLSNIFKNNLGLT
                     AIFDTVDNANVAARQLNYQVRLVTLDGTELRPGGSYSGGANRQNNTVFIKPELDNLKK
                     ELKQAQSKQLIQEKEVAALLEQLKEKQETLAQLKNDGEQARLEEQRADIEYQQLSEKL
                     ADLNKLYNGLQLSSGALEQTTSENEKNRLEKELEQFAIKKEELTTSIAQIKEDKDSIQ
                     EKVNNLTTLLSEAQLEERDLLNEQKFERANCTRLEITLSEIKRDISNLQTLLSHQDSQ
                     LDKEELPRIEKQLLQANKRRENDEEKLVSLRFELEDCEAALDDLAASLAKEGQKNESL
                     IRQQAQLESQCEQLSQQLMIFSRQLSEDYQMTLDEAKVKANVLEDILMAREQLKSLQA
                     KIKALGPVNIDAIAQFEEVHERLTFLNTQRDDLVHAKNLLLETITDMDDEVKTRFKST
                     FEAIRHSFKETFVQMFGGGSANLILTEGDLLSAGVDISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKSSQFIVVTHRKGT
                     MSAADSIYGVTMQESGVSKIVSVKLKEAQEMTN"
     gene            40630..41427
                     /locus_tag="VBE34_000519"
     CDS             40630..41427
                     /locus_tag="VBE34_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7263260.1"
                     /translation="MIKLVATDMDGTFLDENGTYDKKRLANVLKKFKEQGIVFTAASG
                     RSLLSLEQLFADFRDQMAFIAENGSAAVLFNRLAYEQHLSREQYLDIIDHLSKSPYME
                     NNEYVLSGKDGAYILSDANPDYIEFITHYYDNLQKVSHFEDVDDIVFKVTANFTEETV
                     RQAEEWVNQAIPYATAVTTGFKSIDIILSSVNKRNGLEHLCEQYGIRAEEVLSFGDNI
                     NDLEMLEWSGKAIATENARPEVKEIADCIIGHHNDQAVMAYLESMVD"
     gene            41427..42251
                     /locus_tag="VBE34_000520"
     CDS             41427..42251
                     /locus_tag="VBE34_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7263261.1"
                     /translation="MLDIKILALDLDGTLFTTDKKVSEENKVALKAAREKGIKVVITT
                     GRPLKAIGNLLEDLELVSDEDYSITFNGGLVQQNTGKILAKTAMTRQEVEDIHEELYQ
                     VGLPTDILSEGTVYSIANKGHHSQYHLANPLLEFIEVDDLEQVPKDVVYNKIVSVIDA
                     TYLDQQIAKLPDRLKVDYEMFKSRDIILELMPKGVHKAVGLELLTKHLGLDSSQVMAM
                     GDEANDLSMLEWAGLGVAMANGIPEAKATAKATTICNNDESGVAEAVGKYILSEEN"
     gene            42251..43861
                     /gene="ftsY"
                     /locus_tag="VBE34_000521"
     CDS             42251..43861
                     /gene="ftsY"
                     /locus_tag="VBE34_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEO7263262.1"
                     /translation="MGLFDRLFGHKKKDKELEIEASESVVLENEDSVIDKEESSNFSK
                     ESTLNRTSEVPVAEDDSFLELERDTALSESHQPVTSEIHPLESEDTSEIPVKEDDSFL
                     ELEDRAKTKVADTSEVGNVVPDSTTLSDNVSAKSEASFSDEEQSSDSQASEQFSETPL
                     QEEMSSGKTEVQTESEDTSAADAFLADYYAKRKAIEKEISSNSLSTDESELSEAQEVL
                     SQSQADTIKAESQEEKYNRSLKKTRTGFSARLNAFLSNFRRVDEEFFEELEEMLILSD
                     VGVNVATQLTEDLRYEAKLENAKKSEDLKRVIVEKLVEIYEKDGIYNEAINFQEGLTV
                     MLFVGVNGVGKTTSIGKLAHQYKSQGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVVT
                     GEKKADPASVVFDGMEKAVAQGVDVLLIDTAGRLQNKENLMAELEKIGRIIKRVVPDA
                     PHETLLALDASTGQNALSQAKEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVK
                     FIGFGEKIDDIGEFNSEDFMRGLLEGIL"
BASE COUNT        14493 a         6688 c         8973 g        13878 t
ORIGIN      
        1 aaggcctctt acaaagccta ttttatcaga aaatttttaa taagaggtaa aataagttta
       61 cttattaatt tagaattatt ctaaagagct tttttttgat ttttatgtta gacttaatta
      121 taatgattat aaataagaaa taaaggagct taacaatgtc ctatttaacc tttaaacacg
      181 ttgattttaa aacggatgat aagttggttt taaatgatat caactttgca attgatgaag
      241 gtgattttgt ttctattgta gggccatctg gaagtggtaa gagtactgta ttaaaattag
      301 ccagtggttt aatgagtcct actgcaggtc atattttctt tgatggaaaa gatttgaacc
      361 aattagaacc gattgaaagt cgaaaaatga tttcttactg ttttcaaacg ccacatttat
      421 ttggtaatac agtggaagac aatattagtt ttccttacca cattcgacac gaaaaagtag
      481 attatagacg cgttgatgat ttatttcaac gttttgaaat ggaccagtct tacctgaaac
      541 aggatgtcaa aaaactatca ggaggagaaa agcaacgtat tgctctgatt aggcaattat
      601 tgtttgaacc taaagtttta ctcttagatg aagttacttc ggctttagac aatcataata
      661 aagctattgt tgaaaaggtt attaaatcat tacatgacaa aggaataacg attttgtgga
      721 ttacgcatga tgaggaacag agtcgtcgat ttgccaataa agtcttaaaa gttgtcaatg
      781 gtagtattga agcaatggag gttataaaat gagcggtgca aattctattt ctttaatgtc
      841 attactaatg gcatcgtcat tagttttgat tactttattt ttttcttact ggcaaaagtt
      901 aaacctagaa aaagaagtga ttattagcgc tatccgagca gtgattcaat tattagcagt
      961 gggttttttg ctagactata tatttggtta tcaaaatcct attttcacag ctcttttaat
     1021 gctattcatg attattaatg cttcttataa cgcagctaag cgtggtaaag ggattaataa
     1081 agggtttgtt atttcattta tagctattgg gtcagggact ataatcactt tatctgtctt
     1141 gattttttca ggaattttaa aatttgttcc gaatcaaatg attcctgtag gtggtatgat
     1201 tattagtaat tctatggtag caatcggttt gtgctataaa caattattgt ccgaatttag
     1261 aagtaaacaa gaggaagtgg agacaaaatt agctttagga gcagatattt tgccagcttc
     1321 gattgatatt attcgtgatg ttatcaaaac tggtatggta cctacaattg attcagctaa
     1381 aacacttggg attgtatctt tgccagggat gatgacaggg ttaattcttg ctggaacttc
     1441 acctatccaa gcggttaaat accaaatgat ggtgacgttt atgctattag caacgacttc
     1501 gatagcttct tttgtagcta cttatttggc ttataaaatt ttctttaata atcgtaagca
     1561 attagtagta acaaaagatt gattcatttt aataaacagt atgttagtct ggtaagtata
     1621 gtccaggctt ttttggtatc ttaatcttat ttttgatcga aaattgacca agttattgag
     1681 tcgattacgt ttaaatgcta ctatttaata gaaattgttg tagggattat caaaaggagg
     1741 aagaaatgat tttaaaaatt tgtcgtgcag catatagttt acaatgggga ggtgtttacc
     1801 aattagcttt gctggattat cctcgaatta aggcgtttga attggaaagg ataggagctt
     1861 tcatagctta cgagaaacaa tataaaagaa aaactgagat acaatgtgac gataaacatc
     1921 tcctcgcaaa aattgttcat tttttaaaat acaatagttt tacttttccc tatattccca
     1981 aatatagaga agcggcagct acttttaatg aggatggtat tagtttaact tctgattttt
     2041 taagccatac atgtacgatt gaaactgcaa aactaatttt taaagaaggt aaaatcttat
     2101 cagcagttaa agcctttaat aagcctgctg aagtactggt aaaagataag aggaatgctg
     2161 ctggagaccc taaagattac tttgactatg tgatgttgaa ctggtcaaat accaattctg
     2221 gttatcgttt agtaatggaa agattgttag gcaaagcacc atctgaacag gagttaacag
     2281 taggttttaa gccaggggtc agttttcatt ttacttatca agatatcatc aatcatcctg
     2341 attctatttt tgatggttat catcctgcta aaattaaaaa tcagctttct ttagcagaac
     2401 atttagttgc atgtgttatc ccaaaacatt atcaagaaga ttatcaaagc cttgtgccca
     2461 atgacttgaa acacagggtt tattatttag attactgtaa cgaaacactt tatgagtgga
     2521 atcaaaaagt ttatgatttt ctttgtcatt tggaaaataa ataaaaccaa aaagtttaaa
     2581 actttttggt ttaactgcta ttaattggtc tttaaaagac ttaaaaattc ttcaatcata
     2641 ggactaaaag aagtatcatt tcggtaagca ataccaatcg gataaaaatt atttttatcc
     2701 ttaagtggga cccagcataa atcttgaact tggctaatgc tttcaaattc acttggtaaa
     2761 atagtgactg cattgaggtc ttgtaaacta tgaataagaa cttcccagtc attgtgttta
     2821 aagacgatat gggggtcaaa tcctaaagca cgacatttac gaggcaacat ctctcctagc
     2881 atataatgtt cgttgagtga ggcaacttta taatctctta aatcattaag ttcaatttca
     2941 ggaagtgttg caagaggatg cgattttggc atgacaatgc ttactttata tcctttagtt
     3001 gaggtttgca gtggttcgat agtgatatca ttgcgggtac ttggaaagga caataagcca
     3061 atatcgatct ttccttttgc taacaattct tgtaatttac gggaaccatc ttgaatcaaa
     3121 gatacttcaa catttgaatg tgtagccata aaagttgaaa tctgtttcat aaattgaata
     3181 gcaaaaagag aagtaagccc tacacggaca ccttcctttt tttcttgatt taattgctgg
     3241 atctccgtaa ccatttgatt aacattagtc atcaacaatt gtcctttctc atagagaatt
     3301 tctcctgctt cagttaaagc gagttgacga ccattttgta agaataaaat agtatttaat
     3361 tctgcttcta attttttaat ggattgtgaa agtgtaggtt gggtcacaaa aagacttttt
     3421 gcagcgcgtg aataattttt tgcttctgca acagcaataa agtaagtaag ttgtctaata
     3481 tccataatat ctgtcctttt ttcttagtaa tagtatagca caagtctatc aatctgacct
     3541 ttatgattat ttccacaata atattagtaa aagctatact tatataattt tttccaattt
     3601 tccttttgca aatagctatg atatagttaa tatagttagt gaaatcacaa acaatcatta
     3661 ttagtaggag gcatcttatg tcggatgttg tcgaaaaaca aacaactaaa tcgtttataa
     3721 tgaacgtatt aaatggttta gctttaggga ctgttattgt cttgatacca ggagcaatcc
     3781 ttggagaatt aatgaaagct ttactgccaa tgtggagcgg ctttgcaact ttaatcgctg
     3841 caacagcagt tgcaacaagt atgatggggc ttgttattgg tattatggtt ggtctcaatt
     3901 ttaaatttaa tcccattcaa tcagcatcac taggattagc ggttatgttt gcaggtggag
     3961 ctgcaacctt ccttaaaggc gctattatgc taaaaggaac aggcgatatc attaacatgg
     4021 gaattactgc tgccttaggt gtccttttga tccaattttt atctgacaag acgaaatcat
     4081 tcactttgat tgtcattcca acagtaacac tcttactagt tggaggagta ggtcatgtgt
     4141 tattacctta tgtcaaaatg attaccacca tgattggaca agggattgct tcattacttg
     4201 gtttacaacc tgttttgatg tcaattttaa ttgcgatgat tttctgtttc ttgatcgtat
     4261 ctccaatcac aacagttggg attgctttgg caattagttt gtccggaatt ggttctggtg
     4321 ctgctaattt aggtatctgt gctgctagtt ttgggctttg tatggctggg tggtcagtca
     4381 attctaaggg gacagcctta gcgcatgttt tagggtctcc taagatttcg atggctaatg
     4441 tattagcaaa accgaagatt atgctaccta tgatatcttc agcagcaata ctaggaattc
     4501 tcggagctct ctttaatatt caaggaacac cagctagtgc aggttttggt atcagtggtt
     4561 tgattggacc gattaatgct cttaatttag caaaaggtgg atggtcagtt atgaatatgt
     4621 tattgataat tataatattt gttgctgcac caattatatt gaattttatt tttaattacc
     4681 tctttataaa agtacttaaa attattgatc caatggacta taaattagat atttaaggag
     4741 atattagtga tgaaactcaa agtatttaat gttagagaag aagaagcaac attagcacaa
     4801 gattgggcaa accgaaatca tgtggaactt tctatgagtg aaggaccttt gacattggaa
     4861 actgttaatg aggtagaagg ttttgatggg attgctaatg cacagattga gcctttagat
     4921 gatgctattt atcctcttct caaagaaatg ggaattaagc aaatcgctca gcgcagtgct
     4981 ggtgttgata tgtataatct tgagttagct aagcaacatg gtattatcat tagcaatgtg
     5041 ccaagttact caccggagtc tattgcagaa tttacagtga caatcgctct taaccttatt
     5101 cgaaaagttg agcttattag agctaatgtt cgtgaacaga atttttcatg gaccttgcca
     5161 attcgaggac gagtcctagg taacatgact gtggctataa taggaactgg tcgcattggt
     5221 ctagcgactg ctaaaatttt taaagggttc ggatgtcgtg ttattggtta tgatatttac
     5281 cataatccaa tggcagatgg tatccttgag tatgtcaatt cggtagaaga agctgttgaa
     5341 gaagctgatc ttgtgtcctt acatatgcca ccaactgctg aaaatacgca tctatttaat
     5401 ttagatatgt ttaaacagtt taaaaaaggt gctattttaa tgaatatggc acgtggagca
     5461 ttggtggaaa ccaaagatct tcttgaggct ttagatcaag ggcttttgga gggagctggt
     5521 attgatactt atgaatttga ggggccatat atacctaaaa attgtcaagg tcaagacatt
     5581 tcagacaagg atttcctaag actgattaac catccgaaag taatatacac tcctcatgcg
     5641 gcttattata cagatgaagc ggtcaaaaat ctagtagagg gtgccttaaa tgcttgtgta
     5701 gaagtgattg aaacgggaac aactactacg aaagtcaact aaaagatgct taggcatctt
     5761 tttttgtggg gtaacaaaac atatgatgat tatgaccttt aaagcaaaaa aacatcttat
     5821 gtatttatag cttgaaagtc ttttttataa taaaaaaaga tattttttat aaaaatattg
     5881 aaaaaacatc agatgaatag tattatttaa tcataaaagc gtttacaaaa tacaaggagg
     5941 gttctctcat ggagaaaaca atggatatgg aacaacgtga agaagttgta tcttataacc
     6001 gagcgaaagt atggcagtta gttatgtttg ccatgaacaa tgcttctaca aatatatact
     6061 tatttacgtt tatgtttgtt acctatttct caactggcgt tcttggctcg gcagccattt
     6121 ttgtcagtca aattatgggt tatatccgta tttttgatgg ttttattgat ccagctattg
     6181 gtattatgat tgataaaact gatacaaaat ttggtaagta tcgtcctatt ttgattattg
     6241 ggaacgttat tacggctttg tcattgattt ttcttcttgc attgagaggt gtcgatgaaa
     6301 atattcgttt cccattattt atccttgttc taattatcca taaaattgga tactcaatgc
     6361 aacagacaat aactaaagca gggcaaacag cactgacaaa tgaccctaag caacgtccta
     6421 tttttaatat cgttgatgca gttatgacaa catcattaat gacaggcggt cagtttgtcg
     6481 tttcagtttt cttagtacca aaatttggta attttacacc tcaattcttc aatgtcctta
     6541 tttttggaac aattcttatt tcagctattt tagcaatcgt tgcaattatt gggatttggg
     6601 caaaagacag aaaagaattc tttggtttag gagagaatac ccaaaagact gctctaaaag
     6661 attactggaa ggtgttgaaa ggaaataaac cgcttcagat attatccatt gctgcagctt
     6721 tggtaaaatt tgccatccaa ttctttggcg attccgttgt tatggtgcta ttgtttggta
     6781 ttttatttgg taattatgct ttatcaggac aattctcact tcttttcatt gttcctggtg
     6841 ttattattaa tattttgttc tcaacaattg ctcgtaaaaa aggtcttcgt ttttcatatg
     6901 tcagagcaat tcagatcggt atgattggtt tacttgcttt tggtgcagtt ctttacgttg
     6961 gtaagcctgg tgaccttagt ttaacaagcc taaatcttta tactatttta tttatcgtta
     7021 ctaatattat tgcgagatac gcttcacaag cacctgctag tttagtattg actatgggag
     7081 cagatatttc tgactatgaa acttctgaat ccggtcgtta tgtatcagga atgattggaa
     7141 ctatcttctc cttaacagat tcgattgctt cttcatttgc tcctatggta gtaggttttg
     7201 tgttagcagg aattggattt tcaaaatcat ttccaactat cgaaacacca ctgccccctg
     7261 atttaaaaat ggcagctatt agtatccttg tggccattcc atttatagct ttgtctatag
     7321 ctcttctttt aatgaaattt tacaaactag ataaagaaga aatggttcgt attcaagaga
     7381 agattcaagt tatgaaagca gcaactacta aggaacgtgc taaagatatt gctcgtaatg
     7441 ttcctttgac agatatggat tatgttgatg taacaaaata tcctattgat aaagattaaa
     7501 aagtttagct gctcttcaat agagcagcta ttttgttaaa taagagctac cattggagga
     7561 ttcttatggc aaaaattatt tcattaggtg aggttctttt gcgattatct ccacctcaat
     7621 atcacacact gatgcaggct aatcacctaa aatgccaatt cggtggttca gaacttaatg
     7681 tcttagcaag ccttgctcaa ctaggttacc atgtaggatt ggtctcagct ttgcctgata
     7741 atgatttggg caagatggca agtcaattta tattgtcaca gcaaattagt ccagctgcta
     7801 taattaaaaa agaaggacga ctaggaattt attactatga acaaggattt tcggtaagaa
     7861 caaataaggt tatctacgat cgtaactatt catctttttg ggaaagtacc ttgagtgatt
     7921 atgattttac ttctattttt aagggagtgg attggtttca tgtttctgga attactccag
     7981 cactaaccaa ggacttatat gaggtgactc ggttccttat gacaaaagct aaagaaggag
     8041 gtgtaaaagt atcgattgat ctcaattttc gtgagagttt atggtcatct tttcaagagg
     8101 cacgtgagca attatcgcca ctattaggat tattagatgt ttgctttggc ttagagccga
     8161 tttatttagc tggcgaaagt gaagatttaa aagatgagtt gggcttatcc agaccttacc
     8221 ttgatattga attacttgaa aagataaccc aaaagattgt ccaagaatat gggttagact
     8281 atattgcctt tactcaaagg gaaatggaat atactaatca gtatatgtta aaatcatatt
     8341 tgtatcataa taatatgtta tatcaaactg ataaaactgg cgttgaagta ttagatcgtg
     8401 tggggacagg agatgccttt gctgcaggtt taattcatgc gcttctggaa aaagaaactc
     8461 ctcaaagggc cttagaaata gctatggcta catttaaata caaacatacc attcaaggag
     8521 atatcaatat catgacacga gatgatattg cttacctcat tgaaaaagaa acaaatgata
     8581 ttaaacgata gtgtaaaagg agagtgtatg ttatatccat tattgacaaa aacaagaaat
     8641 acttatgatt taggcggtat ttggaacttt aaattaggag aacataatcc aaatgaatta
     8701 ctaccttcag atgaagtcat ggttatcccg acttcattta atgatttgat ggtaagtaaa
     8761 gaaaaacgtg attatatagg tgatttttgg tatgaaaaag tcattgaagt acctaaagta
     8821 tcagaggatg aagagatggt gctgcgtttt ggctcagtga cacatcaagc taaaatttat
     8881 gttgacggtg tattggtagg agagcataag ggaggtttta ctccttttga agttcttgtt
     8941 cctgaatgta agtataataa tgagaaaatt aaggtatcaa tttgtgctaa taacgtttta
     9001 gactatacca cgcttccggt cggaaattat agtgaaatca ttcaagaaga tggtagcatt
     9061 aagaagaaag tacgtgagaa ttttgatttc ttcaattatg caggtgtgca tcgtcctctt
     9121 aagttgatga tacgtcctaa aaatcatatt tttgatatta caattacaag tcgtctatca
     9181 gatgatttac agtcagcaga tttacatttt ttagtggaga ctaatcaaaa ggttgacgaa
     9241 gttagaatta gcgtttttga tgaagataac aaactagtgg gtgaaacgaa ggatagcaga
     9301 ttatttctta gtgatgtcca tctttgggaa gttttaaatg cctatctgta cacagcgcgt
     9361 gttgaaattt ttgttgataa tcaactccaa gatgtctatg aggaaaattt tggtcttaga
     9421 gaaatagaag tgacaaatgg tcaattccta ttgaatcgca aacctattta ttttaaagga
     9481 tttggtaaac atgaagatac gttcattaat ggcagaggtt tgaatgaagc tgctaactta
     9541 atggatttga atcttttgaa ggatatgggg gcaaactctt ttagaacatc ccattatcct
     9601 tattctgaag aaatgatgcg tttagcagat cgaatgggag tgttagtgat agatgaggtt
     9661 ccagcagtag ggttatttca aaattttaac gcttccttag atttgtcacc aaaagataat
     9721 ggtacgtgga atttgatgca aacaaaagcg gcgcatgaac aagctatcca agaattagtg
     9781 aagcgtgata aaaatcatcc tagcgtcgtg atgtgggtag tcgctaacga accggctagt
     9841 catgaagcgg gagcacatga ttattttgag ccattagtaa aactttataa agatttagac
     9901 cctcaaaaac gtcctgtcac cttggttaat attttaatgg caaccccaga tagagaccaa
     9961 gtgatggacc tggttgatgt tgtctgcctt aatcgttact atggttggta cgttgaccac
    10021 ggtgatttaa caaatgcaga agtaggtata agaaaagagt tattagaatg gcaagataaa
    10081 tttcctgaca aaccaattat cataacggag tatggcgctg atacgttacc gggattacat
    10141 tctacttgga atattccata tacagaagaa tttcaatgtg atttttatga aatgagtcat
    10201 cgtgtttttg atggtattcc taatttagtt ggtgagcaag tctggaattt tgcagacttt
    10261 gaaactaatc tgatgatact tcgtgtacag gggaatcaca aaggtctctt ttcaaggaat
    10321 cgccagccga aacaagtcgt caaagaattt aaaaaacgct ggatgactat tcctcattac
    10381 cataataaaa aaaatagtgt aaaatagggg tagttatttt agaagaggtg atgatatggc
    10441 tagaccctta gtagaacaaa cagcagatcg tctattgcat ttaattttag aaagagaata
    10501 ccctgttggt gctaagctcc ccaatgaata tgaattagca gaagatctag atgttggtcg
    10561 cagcacaatc cgagaagcag tcagaagttt agcaacgcgt aatattttag aagtcagaca
    10621 gggctctgga acatatatta gttctaaaaa gggtgtttct gaggatccat tagggttctc
    10681 tctaataaag gacacagata ggttgacatc agatctcttt gaactacgcc ttttattgga
    10741 acctcgaata gcagagctgg tagcttatag aattacagat gatcagttac aattactcga
    10801 aaagcttgtt ggagatattg aagatgctgt tcacgcaggc gatccgaaac atttactttt
    10861 agacgtggaa tttcattcca tgctagctaa atacagtggt aatattgcaa tggatagcct
    10921 tttgccagtc atcaaccagt caattcatct gataaatgcg aattatacaa atcgtcagat
    10981 gaagtcggat agtttagaag cacatagaga aattatcaaa gcaatccgag aaaaaaatcc
    11041 agtagctgca catgatgcta tgcttatgca tatcatgagt gttagacgtt cagcgttgaa
    11101 agcttaatat aagaagaaaa atagctattc ttgaatagct atttttttag tattcttctt
    11161 gacaattcat cagatgactt atataatata gttaaagata acggttacat tttgggaggt
    11221 attatgttaa atcaattgaa aaacaactat ttctttgctg ttattcgagg gaaatcatct
    11281 gagtgaggat gcgctcgaaa tcgcaaagca tgctattttg ggtggtattc ggaatattga
    11341 agtaactttt tcgactcctg aagcaagcaa agtcatcaaa caattatcag atgactttaa
    11401 aaataataaa gaaattattg tgggggccgg aacggtcatg acaactgaac ttgctaaaga
    11461 ggctattgat gctggagcta aatttttggt tagcccacac tttgattcag atattgctaa
    11521 tttagctaat gaaaacaagg tttattattt cccaggctgt gcaacggcaa cagaaatcgt
    11581 ggtagctaga aagtataaat gtcagattat aaagcttttt ccaggtggag ttgtgggtcc
    11641 aggttttatc aaagatattc atggtcctat tccagatgtt gatttaatgc catcaggtgg
    11701 cgtctctgtt tcaaatgtag ttgaatggag aaaagcagga gcagttgcgg ttggtgttgg
    11761 ctcagcatta tctagtaaag tagccacaga aggctatgat agtgttacta aaatagctaa
    11821 acagtttgtt tctgcacttg attagtgagt aagagaagaa tatggcgttt aatactgaaa
    11881 catttatgtt aaaaaatcaa gcagctatac agctgtatga agaggtcaag agacaaccta
    11941 tttttgatta tcattgccac ttagatccaa aggacatttt tgaagatcat atttttgata
    12001 atattgtgga tttgtggctt ggtggtgacc actataaatg gcgtcttatg agggctaatg
    12061 gtatttctga agcagaaatt acaggtccag catccaacct tgagaaattt aaagcatttg
    12121 ctagaacact agaaagagca tacggtaatc ctgtttatca ttggtctgct atggaattga
    12181 aaaatatctt tggtgttaat gagattttga cagaatcaaa tgctgaggaa atttatcatc
    12241 gtttaaatca tttcttgaaa gagcataaaa taagtcctag acgtttaatt gcagatagca
    12301 aagttatgtt tattggtact acagaccacc ctcttgatac tctcgagtgg cataaaaagt
    12361 tggctgctga tgagagtttt aaaacagttg ttgctccgac atttaggcca gatgaagctt
    12421 ttattgaaca tcgacatttt gttgatttta ttactaaact gggtgacatt actcaaaaag
    12481 agattacaga tttttcaaca tttatagcag caatggaaga acgcattgcg tattttgctc
    12541 aaaatggttg tagggctagc gatatcagtt ttacggaaat cgtctttgaa caaactgatg
    12601 aattggagtt gaacgattta tttaataaag tttgtgaagg ctatatccca aatcaatcag
    12661 aaatcagcaa atggcaaaca gctgttttta tggaactttg ccgattatat aaaaaatatg
    12721 gttttgtaac acaggtgcat tttggagctt tgagaaataa tcattctact atttttgaaa
    12781 aattgggagc tgacgtgggt gtggattcac ttggtgacca agtggcttta actgttaata
    12841 tgaaccgttt attggattcc ttggttaaaa aagatagtct gcctaaaatg atttggtata
    12901 atctcaatcc agcttacaat atagcagtag ctaacacttt ggcaaatttt caagctaacg
    12961 aacttggagt gagatcttac cttcaatttg gtgcaggttg gtggtttgca gatactaaat
    13021 tagggatgat tagtcaaatg aatgccttag cagagcaagg catgttggcg aattttattg
    13081 gtatgttaac agattctaga agttttctat cttatcaacg tcatgactat tttcgacgta
    13141 ttttatgtac ctacttagga gaatggattg aagaaggaga agttccagaa gattatcaag
    13201 ctttaggaag tatggctaaa gacatcgctt atcaaaatgc agttaattat tttaagaact
    13261 aaaaaggaag agtgaaatca tggaaatgtc atttaggtgg tatggtgaag acgaccccgt
    13321 tactttagaa aatattggtc aaattccaac aatgaaaggg attgttacag cgatatacga
    13381 tgtaccagta ggagaagttt ggtcacgtga gcgcattcaa cagctgaaag aaaaggtaga
    13441 ggcagcaggg cttaaaattt cggttatcga atcagtacct gttcatgaag atatcaaatt
    13501 gggacgtcca acacgtgatc tcttgattga taattatatt caaaccgtca aaaatttggc
    13561 tgcggaaggt attgatacta tttgttataa ttttatgcca gtatttgact ggacacgcac
    13621 tgatttagca tatcaatatc cagatggttc cacagctttg atatttgatg agacggtttc
    13681 taaaaaaatg gatccagtaa acggtgaatt atctttgcca ggatgggatg ccagctatag
    13741 taaagaagaa atgaaggcta tcatggatgc ctatgctgaa attgatgagg aaaaactttg
    13801 ggaaaatctc acttatttta ttaaacgtat tattcctgaa gcggaagccg taggcgttaa
    13861 aatggccatt catccggatg acccaccata ttctattttt ggtttacctc gcattattac
    13921 tgggttagag gctattgagc gttttgtgaa attgtacgat agtaaaagta atggcatcac
    13981 tttatgtgtg ggttcttacg cttctgatcc ccagaatgat gttttagaga tttcgcgacg
    14041 tgcttttgag ttagatcgtg taaactttgt tcatgctcgc aatattaaat taggtgatgg
    14101 aaagtcattt aaagaatcgg ctcatccatc agaatacggg tcaatagaca tgtatgaagt
    14161 tattaagtta tgccatgaat ttgggtttga gggtgctatt cgacctgacc acgggcgtat
    14221 gatttgggga gaaacaggtc gtccaggata tggcctatac gatagagctt taggagccac
    14281 ttacgtttct ggtttatacg aagctgtgat taaaggttca aaataaggcg atgcttccaa
    14341 atttctcctg ttccaatctc ctgaaagaga aatttggaaa gttattatca taatatgatt
    14401 gtacttagct aagagtagaa tacttataga agttaatgag aggaataaga tgtcaaaaac
    14461 aattacattt accaataaag ttgtcgtcat aacaggtgca ggcggtgttc tctgtggtta
    14521 tatggctaaa gaatttgcga aagcaggcgc taaagtggca ttattagatt tgaatcaaga
    14581 agctgctcaa acatttgctg atgaaattgt agaagaaggt ggtatcgcta aggcttataa
    14641 agctaacgtt ttgtctaaag aaaaccttga agaagtgcat caagccgttt tagaggatct
    14701 tggaccaact gatattttag ttaatggtgc aggaggcaac aatcctaaag ctacaactga
    14761 taatgaattt cacgaattag acttaccttc agaaacaaaa accttctttg agttagatga
    14821 agctggtatt agctttgttt ttaacttaaa ttatttagga acattattgc caacgcaagt
    14881 atttgcccaa gatatggttg gacgtgaagg agcgaatatt attaatatct cttcaatgaa
    14941 tgctttcacc cctctgacca aaataccagc ttattctgga gctaaagcag ctattagtaa
    15001 cttcacacaa tggctagctg ttcatttctc aaaagttggt atccgttgta atgctattgc
    15061 acctggtttc ttggtaacaa atcagaaccg aagcttgtta tttactgagg acggtcagcc
    15121 aactgcgcgt gctgagaaaa ttcttaataa tacaccaatg ggaagatttg gagaagcaag
    15181 tgagctgatt ggcggattgt tctttttagc agatgaaaaa tcatcaagtt ttgttaatgg
    15241 cgttgtcctt ccgattgatg gtggatttgc tgcttactct ggtgtttaat ttacaagata
    15301 atagccactt aacacataag agattattga gttgagtggt tttttaagga aatttgttta
    15361 atgatcttac cactaaatga tgggtaggtt gctaattgga aaatcctcat aagttagata
    15421 aatttagaaa ctggagataa tatggtagat acaaatcaca aaatcgcttt ttgcatagat
    15481 tctgatggtt gcgcaatgga tacaatgaca tataagcatc aactcttttt tggtccaatt
    15541 gctgcagata tatttgggat atcagaacaa gaaagaacaa gttttttaaa agagtgggat
    15601 agagtcaatc tttattcaag aactcgtgga gttaatcggt ttgtgggatt ggttatgggc
    15661 cttgagtttg ccggagtaga acatattgat aaattaaaag aatgggtcga aacaacagac
    15721 tcattgtcca ataattcctt agagttagcg cttgccaacg attttagtag agatttagag
    15781 aaggctttag aatggtccaa tgaagtgaat cgccaaatca aaaattatga gggagattct
    15841 ttagcgtttt caggtgtgtt agaaagttta gaacgattaa gtcaatttgg taatgtttat
    15901 gtcgtgagtt ctgctaacaa ggaagctgtt tggcaagagt gggaagaaca gggattgcta
    15961 aagtatgttg atgatatcta ttgtcaagat agaggtaaaa aagaagatgt tatcgccact
    16021 ttaattgcaa gtggttactg ccgtgaaact attttgatgg taggagattc cctgggtgat
    16081 ctggctgcag cagaacaaaa tggtattgca ttttacccag tacttgttgg taaggaagtc
    16141 aaatcatggg aaattcttag agaagatatt ggtgaagctt ttgcaaaagg tcaattcgaa
    16201 cagcaaaggc aaaaagaatc tattaatacc ttttgggcta atttagataa ctaatagaaa
    16261 ggataggcta tgacacattt agttgactta acaaagaaac ccttcaatct aaatcaagaa
    16321 gcaattgagt ggattgaaaa aacaattaat gagatgacat tggatgagaa aattgggcaa
    16381 ctttttttca atatgggagc tagtcgttca gaagaatatt taacagatgt tttggatcgg
    16441 tatcatattg ctgctgttcg ctataataga ggtagttcaa gtgaaatcta tgatcagaat
    16501 ttaattttac agacaaagtc gaaattgccg atgttgattg ctgcaaatac agaagctggt
    16561 ggagacggtg ctgtcacgga tggaactaaa gttggcgatg aaataaaagt ggctgctaca
    16621 aatgatccga aatatgctta tgaaatggga agaatcgcag ggatggaggc atctgctgtg
    16681 ggttgtaatg cttcattttc tccaattgta gatttgacac gtaattggcg taatcctatc
    16741 atagctagtc gaaattgggg tgctaacgtt gatcaaatta tttcactttc aaaagagtat
    16801 atgaaaggaa tcatgcaata taatattgta cctttcgcta aacattttcc aggggatggc
    16861 attgatgagc gtgatcatca tttgtcattt gcttccaatc ctatgagtaa agaggagtgg
    16921 atgtcgacat ttggccgcat ttatggtgaa ttggctgacg caggtttacc aggagtcatg
    16981 gcaggtcaca tacatttacc aaacgttgaa aaagaaatgc atccagagcg agaccttgat
    17041 gatatgttac cagcttcatt gaataaaaca cttttagatg agttattgag aggagagtta
    17101 ggttataatg gagctattgt aacggacgct tctcatatgg ttggaatgac tgcttcaatg
    17161 ccaaggagag atttgttgcc aacggcgatt gaagcaggtt gtgatttatt cttattcttt
    17221 aatgaccctg atgaagacat tcaatggatg aaagaaggtt acgaaaaagg tattctaaca
    17281 gaggaacgtc tccatgacgc tttacgtcgt actctcggtc taaaagcgaa attaggcttg
    17341 cataactatg agggacgtcg ccaagaactc tttatgccta aagataaggc tatggcttta
    17401 atcaacacat tagagtcgca aaaaatagct gatgaagttg ctgataaagc agtaactctg
    17461 gtaaaagata aacaaaaaga tatctttcca gtaaatccag aacgttatcg tcatatctta
    17521 cttgttaacg ttgaaggata taagggaggt tttggtgcga tgattgctgg aaacaagcaa
    17581 cgtgcatcgg attacatgaa agagttactt gaggcacgtg gccatgaagt aactgtctgg
    17641 gagtctaccg aagagcgtat tatgaaattg ccacaagagg agcgggcagc agctatcgct
    17701 aatgtttacg cacaaaaaca gccaatcgca aatctaacag aacactatga cttgattatt
    17761 aacttggtag atgtcaatgc tggaggaaca acacaacgta ttatttggcc agcagctaaa
    17821 ggcacccctg atcagccttt ttacgttcat gaaataccat ctattgtgat ttcagtacaa
    17881 catgcatttg ctttagcaga tatgccacag gtagggactt atattaatgc ttatgatggg
    17941 cttcctagca cgataagtgc agtagttgcg aaattggctg gagaatcaga atttacagga
    18001 gtttcaccag tagatgctta ctgtggttta attgatacac atttatggcg ccaatgctaa
    18061 tagaaacaag atgatgtact tttatgtaac ataaaaaaag agcttaaagc tcttttttta
    18121 tccttcaaaa tagagtagtt ccttgggtgt tttggtaaat acttcaaagc cggttttagt
    18181 cacataaccg cagtcttcaa ttcgcacacc aactttatct ggtatataaa tacctggttc
    18241 aacagagaag cacatgcctt cttgaatctc catatcgtta ccagccataa tagatggaaa
    18301 ttcatggaca tccataccta atccatgacc aaggcggtgg ttaaagtatt gtccataacc
    18361 agccttttca ataacatttc ttgctgctgc gtcaacttca gaagcaagga ctcctggctt
    18421 aataaaatca atagccgctt gatgtgcttc aagacataaa tggtaaatat cttttttgaa
    18481 ttgatcaggt ttccccactg ctactgtacg ggtcatatct gacgtataac ctagtgtttc
    18541 tacacctaaa tcaaataata acaaagcatt attttcgatt ttattagttc ctgggatacc
    18601 atgtggattt gctgcattat tgccagtcaa aaccatagtg tcgaaactca ttttattgat
    18661 accttgtttt ttcatttcaa attcgatttg agcgataata tcggtttcag tgttgtttag
    18721 cgagatattg tcaaatccaa cttgaacagc tttatcagca aattctccag cgactaacat
    18781 tttctcaatt tcatctcgtg atttaattag gcgcatgttt tgaatataag gtgttaggtt
    18841 ttcaaaatga ccatcaaaaa cggtttggag accttggaat ttagtaacat taagattatc
    18901 aaattcggct agaactttag aaacagaaaa ggatggtaag ttgctagcta ttttttgcca
    18961 aggattttct gaatctatgt aaccaaagac gggaaatggt acactttgtt ttgcacgcga
    19021 tacttctagt gctggtacaa ataaaatagg atcgcgatct tcatagacaa ataaaaacat
    19081 ttgtctttca tggggatcgc aaaagaaacc tgttaaatag ttgactgtca caggatcaga
    19141 aaagacagca agttcagctt ggactgaatg taagtgatgg cgtatacgat taagttttga
    19201 cataataacc cttcttccta aaaggaactt agtctagtta ggctagtttc ttatgcctat
    19261 tatttttcca aaaaaatagg aaaaaatcaa gtttagttag tattttgaaa gtttttcata
    19321 aaaaagcttt ataaaagctt gaaagtgttt ccaaaaggtg ataaaatatc agtgaaagcg
    19381 tcattttcaa atagaaagga agttaaatca atatgaatac agatgatacg attacgattt
    19441 atgatgttgc ccgtgaagca ggtgtttcga tggcaactgt tagtcgtgtg gtaaatggaa
    19501 ataaaaatgt caaggaaaat acacgtaaga aagtccttga agttattgat cgtcttgact
    19561 atcgtccaaa tgctgtggcg cgtggtttag caagtaaaaa aacgacaact gttggtgttg
    19621 tcattcctaa tatcgcaaat agctactttt caattttagc gcgtggtatt gatgatattg
    19681 cagcaatgta caaatataac attgtccttg cttcaagtga tgaagacgat gataaagagg
    19741 ttaatgttgt taatactttg tttgctaagc aagtagacgg tattattttt atgggacacc
    19801 acttaactga aaagattcgt gcagagttct ctcgttcacg cacaccgatt gttttagcag
    19861 gtactgtgga ccttgagcat caattaccaa gtgttaatat cgattacaaa gcagcagcag
    19921 ttgatgttat tgatatttta gctggcaatc ataaagacat tgcttttgta tcaggaccac
    19981 ttattgacga tattaatggc aaggttcgtt tggcaggata caaagaaggt ctcaagaaaa
    20041 atggcttaaa ctttaaagag gggttggttt ttgaagccaa ctaccgttat gctgaaggct
    20101 ttgcattagc tcaacgtgtg attaatgctg gagcaacagc agcatatgtt gctgaggatg
    20161 agttagcggc aggtttgtta aatggtcttt ttgaggcagg taaacgtgtt cctgaagatt
    20221 ttgaaattat cacaagcaat gactcgccaa tcgcacaata tacacgtcct aacttaactt
    20281 ctatcagtca accggtctat gatttgggtg ctgttagcat gcgtatgcta acaaaaatta
    20341 tgcacaaaga agagttggaa gaaaaagaaa tagtgcttaa tcacgggatt gttaaacgtg
    20401 gcacaacaaa taattagtgt tagagtatgt tatttgaaaa atagtggtag aatggtagta
    20461 gcttagcgct taactttctg atacttaaca tgatatattg agaggattag ggtagcttag
    20521 cttcctaatc tttttttaga ggtgtaatat gacaaatgaa ttgataatgc aagcttttga
    20581 gtggtattta cctagtgatg ggaatcactg gaagaaatta gaggagtcta tatcagacct
    20641 taaaaaactt ggaattagta aaatctggtt accaccagca tttaagggaa ctagcagtga
    20701 tgatgtagga tatggtgttt atgatctctt tgatttagga gaatttgacc agaatggaac
    20761 aattagaaca aaatatggta ggaaagaaga gtatctaaag cttattaagt cgttaaaggc
    20821 aaatggcatt aaaccgtttg cagatatcgt tcttaaccat aaagccaatg gtgatcataa
    20881 agaaaaattt caagtcatca aagtcaatcc tgaaaatcgt caagaagcat taagtgaacc
    20941 ctatgagatt gaaggatgga cgggatttga tttcccaggt agacagggtg agtacaatga
    21001 ttttaaatgg cactggtacc actttactgg tttagattac gatgctaaaa ataatgaaac
    21061 agacatcttt atgattgtag gcgataataa aggttgggca gacgatgatt tgattgatga
    21121 tgaaaatggt aatttcgatt atctcatgta caatgacatt gattttaagc atcctgaagt
    21181 tataaaaaac ttacaagatt gggctaagtg gtttattgaa actacaggca ttgaaggttt
    21241 tcgtttggat gctgtgaaac atatcgattc ttactttatt caaactttca ttaatgatat
    21301 tcgtacaaaa ataaaacccg atttagaggt ctttggcgaa tattggaaat ctgaccaaac
    21361 atcaatgaag gactatttag aggcaacaca atttcaattt tctcttgttg atgtcacctt
    21421 gcatatgaat ttttttgatg ccagtcacca aaaccgtgat tttgatatgc gaacaatttt
    21481 tgatgatagt cttgttattg ataatcctga gtatgctgtt acattcgtag aaaatcacga
    21541 tacgcaaagt ggacaagctc tagaaagtag agtggaagat tggtttaaac ctttagctta
    21601 tggcttgata cttttacgcc aacaagggac tccatgtctt ttttatggtg attactatgg
    21661 cattcaagga gaatttggcc aaccatcctt taaagaggtt attgataaaa tggctgagct
    21721 acgccaaaac tatgtatttg gcgaacaagt cgattacttt acgcattcaa attgtattgg
    21781 ttggacttgt cttggggatg aggaacataa ttcttgttta gctgtggtcc taacaaatgg
    21841 tgatcaggga tggaaacata tggaagtagg ggaaatttat gcaggcaaaa cttttgttga
    21901 ttatttaggc aattgtgaac aagaggttgt tataggagat gatggttggg gtgatttttt
    21961 agttgcatct gcgtctatct cggcttgggt ccctaaaata gaggaagcta actgataaat
    22021 caggtgaaaa aaaaggtaaa atgagatata ataagaccta tgaaagtttt actgtattta
    22081 gaggctgaag aatacttgaa aaagtcaggt atcggtcgtg ctatcaaaca tcaagagaaa
    22141 gctcttcaaa ttgctgggat agattatacc actaatccta cagatgactt tgaccttgtt
    22201 catatgaata cctatggtat tagaagttgg cttttgatga gtaaagctaa aaaaactggg
    22261 aaaaaagtaa ttatgcatgg ccattccact gaagaggact ttagaaattc ttttattggc
    22321 tcaaatttag tatccccgct ttttaaatgg tacctttgcc gtttttatca aaaagcagat
    22381 gccattataa ccccaactga ttattctaaa caactaataa aagcatatgg catcaagaaa
    22441 ccaatttttg ttttatctaa cgggattgat ctaagtcgct atcagaggtc agaaaagaaa
    22501 gaagctgcat ttcgtcacta ttttcactta agtaaggatg ataaagttgt tatgggggcc
    22561 ggtctttatt tcatgcgtaa gggaattgac caatttgttg aagtagctgc aaaaatgcct
    22621 gatattcgtt ttatttggtt tggagaaaca aataaatggg ttattcctcg aaaggttagg
    22681 caaatcgtta ccaagcaaca tccctcaaat gttacttttg ctggttacat aaaaggtgat
    22741 gtttatgaag gtgccatgag tgctagcgat gctttcttct ttccgagtcg tgaagagaca
    22801 gaagggatag tagttttaga ggctttagct agtcatcagc atgtggtttt aagagatatc
    22861 cctgtgtatc acggttgggt gaccgaagac agtgttgaat tagcgactga tgttgatggt
    22921 tttgttgaaa agttggataa agtcttatct ggtaagagtg ataagattaa agaaggttat
    22981 cgtgtggctg agagtcgaag tatcgaacga attgcccatg aattggcaag tgtttatcaa
    23041 aaagtaatgg agttgtaaga tgcgtatagg tctatttaca gatacatact ttccccaagt
    23101 ttcgggagtt tcaacaagta tccgaacttt aaaggaaggg ttggagaagg aaggacacga
    23161 agtttatatt ttcacaacta ccgaccgtaa tgtcaaacgt tttgaagacc ctacaattat
    23221 tcgtttacca agtgtccctt ttatttcttt tacagatcgt cgagtagttt atagaggttt
    23281 aatttctgct tataggatag ctaaagacta cgaacttgat attattcata ctcaaacaga
    23341 attcagttta ggacttttag gaaaattagt tgcaaaagct ttaagaatac ctgttgtgca
    23401 tacttatcat actcaatatg aggattacgt tggttatatc gcaaaaggca aattgattaa
    23461 gccaagtatg gtcaaatata tcatgcgtac ctacctaagt gatttggatg gtgtcatatg
    23521 tccttctcga attgttctca atcttctaga tggttacggt gtcaaaattc caaaacaagt
    23581 tattccgaca ggtattcctg ttgagaacta tcgtcgagag gatatctctg aagaaacaat
    23641 caagaatcta cgaacggagc ttggccttgc ggataatgat acaatgctat tgagtttatc
    23701 acgggtatct tttgaaaaaa atattcaagc aatcttgatg catttatcag ctgtcgttga
    23761 tgaaaatcct cacgtaaaat tagtgatagt tggagatggt ccttatcttt cagatctaaa
    23821 agaacttgtt cactctttag aacttgaaaa ttcagttatt tttacaggta tggttgagca
    23881 tagccaagtg gctatctact ataaagcatg tgatttcttt attagtgctt caacaagtga
    23941 aacgcaagga ttaacttaca ttgagagtct ggcgagtgga cgtccaatca ttgcgcaaag
    24001 caatccttat ctagatgatg ttatttcaga taaaatgttt ggaactctgt acaagaagga
    24061 aagtgatttg gcagatgcta tcctagatgc aatagcagag acacccaaaa tgactcaaga
    24121 agcttatgaa cagaaattat atgagatttc tgctgagaat ttcagcaagt ctgtatatgc
    24181 tttttaccta gactttctga tttcacaaaa agctagtgtg aaagaaaaag tctctctgac
    24241 gataggaaat aaggatagcc attctactct tcgttttgtc agaaaagcgg tatatttacc
    24301 taagaaagtt tttaccttta caggaagagc ctctaaaaaa gtggtaaaag cacctaaacg
    24361 tcgtatttca tctattcgag attttttaga ttgagacgaa taaaattagt aaatgtcttg
    24421 aaattataaa aaatattgtt ataataaaaa atgaagacat gttaggttct aatagatttc
    24481 aagagcgagc actggttggt ggaaagagtg caattagaga acttagtact acttccttga
    24541 ttttatgtaa acataaaagg gtggttccct tatcgccact agaggtctga cacaagtata
    24601 ctcgagtttt tgtgttggat aaattgaggt ggaaccacgt agtctttacg tccttttgga
    24661 tgtaaaggct ctttttttat aaaagaagtg gttgcataaa agaggcagat tgcttttcta
    24721 atgttatact tattatccct atctaggata atgtggaatt caatcaaagg tcaatccctt
    24781 tgtttattag tgaattaatg tgtttaggaa ttggtatcac cctaacagtt attaagaaaa
    24841 ggagaacaag atgattaaaa tcactttccc agatggtgca attcgtgaat ttgaatctgg
    24901 catcacaaca tttgaaattg cacaatcaat ttctaactct ttagctaaaa aagctttagc
    24961 tggtaaattt aatggtcaac ttattgatac aacacgtgct atcgaagaag atggtagtat
    25021 tgagattgta acacctgacc acgaagatgc tttaggagtt cttcgtcact cagcagcgca
    25081 tttatttgct caagctgcaa aacgcctttt tccagactta tgtttaggtg taggtccagc
    25141 tattcacgat ggtttttact acgatactga taataaatca ggtcaaattt caaatgatga
    25201 tcttcctcgt attgaagaag aaatgaaaaa aattgttaag gaaaatcacc cttgtattcg
    25261 tgaagaaatc agtaaagaag aagcgttaga actttttaaa gatgaccctt ataaagttga
    25321 attaattagt gaacatgctg aagatggttt aactgtttat cgtcaaggtg aatttgtaga
    25381 cctttgtcgt ggacctcatg tcccatcaac aggacgtatt caagtctttc atcttctaaa
    25441 tgtcgctggg gcttactggc gtggtaacag tgataatgct atgatgcagc gtgtctatgg
    25501 tactgcttgg tttgataaaa aagatttgaa agcatatctt aaacgccgtg aagaagctaa
    25561 ggaacgtgac caccgcaaat taggtaaaga acttgattta tttatggtta atcctgaagt
    25621 aggtcaagga ttgccattct ggttgccaaa tggagcaact attcgccgcg aattggaacg
    25681 ttacattgtt gataaggaga ttgcttcagg ttatcaacac gtttacacac ctccgatggc
    25741 atctgttgaa ttttacaaga cttctgggca ctgggaccac tatcgtgaag atatgttccc
    25801 aacaatggat atgggagatg gggaggaatt cgttcttcgt ccaatgaact gcccacacca
    25861 tattgaagtt tataaacatc atgttcactc atatcgtgaa ttgccaattc gtatcgctga
    25921 acttggtatg atgcaccgtt atgaaaaatc aggggccctc acaggattgc aacgtgtacg
    25981 cgaaatgaca ctcaacgatg cccatatttt cgttacacct gagcaaatta aagatgaatt
    26041 tcttaaagct cttaatctca tagcagaaat ttatgaagat tttaacttaa cagattaccg
    26101 tttccgctta tcttatcgtg acccagaaga taaacataag tattatgata acgatgagat
    26161 gtgggaaaat gcacaagcta tgctaaaaga ggctatggat gattttggat tagattattt
    26221 tgaagcagaa ggcgaagcag ccttttacgg tcctaaattg gatatccagg tgaaaactgc
    26281 tcttggtaat gaagaaacat tatctactat ccaactagat ttccttcttc cagaacgctt
    26341 tgatcttaaa tacatcggtg cagatggtga agaacatcgt ccaattatga ttcaccgcgg
    26401 tggtatttca acaatggagc gatttacagc tattttaatt gaaacttaca aaggtgcctt
    26461 tccaacttgg cttgctccac aacaagtttc tgttattcca atttcaaatg aagcccacat
    26521 tgattatgct tgggaagtgg ctcgtgccct taaagatcgt ggtattcgtg ctgaagtgga
    26581 tgaccgtaac gaaaagatgc aatataagat tcgtgcggcg caaacccaaa aaattccata
    26641 tcaattgatt gttggtgata aggaaatgga agaaaaagcc gtaaatgtgc gtcgctatgg
    26701 aagtaaagcg actgaaacaa aatctataga ggaatttgta gaaagtattt tagctgatat
    26761 tgcaagaaaa tctagacctg acgaagttaa ataaatttta ttagtcatta taaaaatcat
    26821 taataaatga tgtgaactga ttactgtatg atgcagaatc agttttttag atgctaattt
    26881 tttcttgatg tttaattata agctaattat agataaataa tctcatataa cattcacaaa
    26941 attagatttt ttggtataat taatatgatt gacacataaa ggaaaatagt attaataaaa
    27001 aggggtatgg agcattgcga acagtattag tagtgcaagg tgatgacgaa acaatagagt
    27061 tattaaggtc gtatttagag ggagctcttt ataaggttgt gatggctagt gatggtgagg
    27121 aggctttttc tttatttcag caacatcaga ttgatttagc aatcatagat attacgctac
    27181 ctaaaataga tggatatgag ttgacgcgac tcattcgtca agatagtcag attcctatta
    27241 ttatgctagc agctaagaca acagatatgg acagaatatt aggattgaat attggagcag
    27301 atgattttat cacgaaaccg tttaattctt tggaggtttt agctcgaatt aattcgcaac
    27361 tgcgtcgtta ctatgaattc aatagtcttg ccaaacctaa aaatcaattt ataaagatag
    27421 gggaattgga actagatgag gaacatgttg aactaactaa aaatggtaaa catatcaagt
    27481 tgactgctac agaatttaag attcttcata tcttaatgtc atcaccagga aagatttata
    27541 ctaaaacaca actctatgag aagattaatg gtaggtattt agaaggtgat gagacaacga
    27601 ttatggtgca tatttctaat atccgtgaca aaatcgaaga tgatagtaaa tatcctaaat
    27661 atataaaaac acttagagga gtagggtata aaatcgaaaa accgcagtat cttgtttaaa
    27721 aacataatgg ctagtgagag ccatttttta gtgaagatat ttcttggttt taagctttcc
    27781 tttttgctat aataagtaaa attggagggg agagatatgg caaagaccgc tcttaatatt
    27841 ccttttacag ttgaagggat agaaaattat tatcaaaata taaaaatgat actgtcttta
    27901 gcagataaat ggagcatacc taaagatgat ctattactct ttttaaaggg actcaaggct
    27961 attgatgata taaaagttgg ctctcctatt tttaagtatt tttggacttc tttaagttta
    28021 caagcacccc tcaaagcttt agaattcgtg ttagagcagg ctaaaatgcc aactgaattg
    28081 tcaggagaat tatcagaaac acaatacttg gtcgcacagt tttcagatga gttagctccg
    28141 catgatgatt tctggatagc cttatcacaa gttatttatg attcttttcc tgggaatagt
    28201 ctagccgaag atactgtatt aaataggaaa ctccatcaat tccgctattt gatttctagt
    28261 cagcaagcac agtatgttcg acgatacttt aaagatgttg gtatgactga tagagatgct
    28321 ttagtaaact acctatcatg tttaagagaa ccagatagta tcgcctacta tgaatctgct
    28381 cgcttgcata ataaacgtcg gagaaatggt gaaatctttg gtttcccaga tgatgagccg
    28441 gttattaata gtaagctttt aatttcattc catacggaat ttattattga tgacaaagga
    28501 aactttttaa acgagattga cgcagaagta atcacacgta atggcattat caacggtgct
    28561 agctttaatt atgcttttaa aaataataca cgtcataaag aattagatgt tgatcctgta
    28621 aagttagacc caaaatttcg aaacgatatg actagaggtt atagatcgcc aaatctatct
    28681 agaaggaaat ggtttttctt taaagaagag gattacgatt gtagttattt caataaaaaa
    28741 ggatattacg catttggaag gcgtagtgct aagcaatcgg ttgataaaca agtcaaatac
    28801 ttgaagaagg ctgtgcagaa aatgcgcatc aactgataca tatactatta aaagatacca
    28861 tgatactatg gtatctttta atttgcggta ttaatgtcaa atggttttta ttgaccacat
    28921 atttttataa acgagtggtc aaaattgaaa aagtgtctag cttatgttaa aatacaattg
    28981 aataatagaa agaaggtgct gtatgattaa gaaaaataaa gtttttttag gagttctttt
    29041 agtccttgtt gttatccttg gaggtggtgt attattttac caatcacaat tccaaaagac
    29101 aactaatcaa gcgttagcta ttgcttataa agatgctaaa gtggctaaga aggatgtcat
    29161 acatcaaaaa attgacaaag aatttgaaaa ctttagaggg agctacgaaa ttgaattcaa
    29221 taccaaaagc gcagaataca gctatcatgt agatgctaag actggacaaa tccttgagcg
    29281 ggacatggat aataatggct tttcaaaatc gacttctcag tcaagttcat cgtcaagtca
    29341 aaaatctcat aaaataagtc aagaagaagc caaaaagatt gcatttaaag atgctaatat
    29401 agaggaatca gaagtcagta atcttaagat caaagaagaa attgaaaatg gaaaatcagt
    29461 ttatgatatt gactttgtgg acctgaaaaa taaaaatgaa gttgattatc aaattgatgc
    29521 agaaactgga aaaatcattg aacgttctag agatcatatg aatgattaat ttaaataaga
    29581 tataaaaaaa cgaaggtcta aaaggccttc gttttaatta ctcagtagct tgctcccaac
    29641 gttttgaaag tttacgtgaa aaacttgaga tgataaagtt tgtaatgaaa tagacagcag
    29701 ttataatagc gtagagtgta aaaacttgtc cagcttcgaa atatcttccc attagaattt
    29761 gactttttcc gaagatttct tgaatagcta taacagaata aagtagtgat gtatctttta
    29821 taaccgttac aaactgagaa ataatggctg gcaacatttt gcgaaaagct tgtggaaaaa
    29881 taataataat gaaaacttgc aaataagtga atccttgaga aagtccagct tctgtctgtc
    29941 ctttatcaac tccatttaaa cctccccgaa taatttctgc taaagcagcg gaggtaaaaa
    30001 ttgtaaacga agtaatacca gcgctgactg atttcatctg gaatactaag aaaatgataa
    30061 aaatccaaag tagatttgga acatttctga caaattcgat atagatacta gctattaatt
    30121 tccagatagg atttttgcta tttctcataa ttgctaatag agttccaaaa aacattgata
    30181 gtaaaatgga aataaaagat atatataagg ttaaaccgaa cccttgaagg agaaatagta
    30241 tatttgttag tgttaagact gatgacatat caaacctcct taagaataag ttttcttatt
    30301 taattcttct tgttttcttg cccagcttgc tagtgggaaa caaataacaa aatagaagat
    30361 agcagctcct gcaaaagctg ggatataatt agtcgtatca taagcccagg ccttagctaa
    30421 aaacatgata tcggctccag aaatgattgc tacggtagag gtattcttga ttaggttaac
    30481 aacttggttg gtcattggtg gtaaaattgt tctgatagct tgaggcaaaa tgataagttg
    30541 catggtttga ttagctgtga atccttgagc taaagcagcc tctgtttgtc ctttagggac
    30601 agcttcaatt cctgagcgaa taacttcaga aatataggca ccatggtata ggccaacgca
    30661 caaaacagcg gtgaaaaaag cactaatcat aatatgacca ttactaatga ttgctagtcc
    30721 atagtagaca aaaaccattt gaaccaaaag aggtgtgttt tgaaaaactt caacatagat
    30781 acgtgatatc aacttaccaa ctttactttt agaagttgat aaaccaccag agagaattcc
    30841 taatatcaga gcaagtagga gtgcaccaaa cgaaatacct agagtatata agaagccttt
    30901 aaagaatagg tcaaaatggt taaagaaagc gccccacctt gaaatggcaa aaggactgat
    30961 gtttaataaa gtcataaaaa atcctcccta ttcttttttt actttagcgg gtttaagctt
    31021 aaatttttga taaatgtgtt ttaaactacc atctttttcc catgatacca gtaacttatt
    31081 aacgtaggga atcaagactt tgtcctgttt actggttgca ataccgtatt cttgcgtatt
    31141 gaacccttct tttaatatgg ttgtatgagg gctaaggtag ccgctaagga tagatttatc
    31201 aactgaaaaa gcatcaatac gattagcgta cagcgatatc gctaattctg ggaaactccc
    31261 taattgagca tagctaaatt ttagcttgtg ttctttagca tatttttcta aattgacttt
    31321 tgtagtggca ccttgagcca caccaatgtg tttaccatcc aattctttta tagttttgat
    31381 atgactattt ttacgcacca aaaatccaat ttcatcgtga taataagctt tagaaatatt
    31441 atagttagct tttctttcgg gcgtgatagt gtatgttgcg attagaatat caatttgccc
    31501 attgtccatc agaggctctc tggtttgtgc agttgtcggt acaaaaactg gtttgacgcc
    31561 taaggatttg gcaatttttt tggcaatatc aatttccatt ccttcgtatt gattcgtctc
    31621 agcattataa taaccaaagt taggcacatc ttgcttgact ccaactttaa ggacacccga
    31681 ttttttgatt tctgataatt gattagctga cgttgagttg ggataaaata acaggaaaat
    31741 gaaaatacag gataatagat agagcgttaa tctttttcgt ctcagcatat ttcgtcctcc
    31801 taaaggtcag tactggtatg gttgataatg tttcttaaaa attgtttggc acgtggttct
    31861 gtgggattat caaaaaagtc ttgcacatcc gtagtatcta ctaaaatttc cccgtcagcc
    31921 ataaaaataa tacgatcagc aacttctcgt gcgaatccca tttcatgagt tacaacgacc
    31981 atattcattc cgtcatttgc caatttttgc atcactgaaa gaacgtctcc gatggtttcg
    32041 ggatcaaggg cagaggtagg ctcatcaaag aggaggagtt ttggatgcat ggcaagccct
    32101 ctagcaatag caattctttg tttttgacca cctgatagca tagatgggta ggaatctttt
    32161 ctttcccaca tattgacaaa ttttaaatat ttttcagcaa tttccatagc ttctttttta
    32221 gattgtttta atactttaat aggagctaat gtaatatttt ctaacacggt tttgtgggga
    32281 taaaggttaa aatgttgaaa aaccatacca acttctttac ggaggttgac caattcttta
    32341 gatgaaatat ttgctaattc atgtccattc acgaccaaac taccatcgtc aatagattcg
    32401 agagcgttca ttgttctaat gagtgttgat ttacctgatc cagaaggtcc taatagaaca
    32461 accacttgcc ctggctctat ttccaaatta atctgacgaa gggcgtgata atctccataa
    32521 tacttgttaa cattcttata agatatgagt gacataagaa cctcctaaaa taacagttta
    32581 ttgttaggtt acctaacatt atagataagt gtatcacctg tttgtgagct tgtcaataaa
    32641 ttcagaatat taaagagtag gattgattta aacattgcta tttttagagc ggtatttcta
    32701 gtaaaaatat tacgttctcg tatcatttat ttttcagttt aataggatgt attgaaagtt
    32761 tccaattaac taggaaatct gttataatta gtgataatag tggtgcttta ggcccatatt
    32821 atcatatttt cggtcacttt tatttttcag tttatggatg gaagtgggag accagaaagt
    32881 aatagaggta aaatatgaaa aaaatactta tcgttgatga tgaaaaacca atttcagata
    32941 ttatcaaatt taatttaacc aaagaaggat atgaaacggc gacagctttc gatggccgtg
    33001 aagctttagt acaatatgca gaatttcaac cagatttaat cattttggat ttaatgctcc
    33061 ctgaactaga tggtttagaa gtggctaagg aagttcgtaa aaccagccat attcctatta
    33121 ttatgctttc cgcaaaagac agtgagtttg acaaagtcat tggtttagaa atcggtgcag
    33181 atgattatgt tactaaacct ttttcaaatc gtgaactttt ggcacgggtc aaagcacatt
    33241 tacgacgtac tgaaaatatt gaaacagctg tagcagaaga aagtgctcaa aatgcttctt
    33301 cagatattac tattggtgag ttgcaaattt taccagacgc ctttattgct aagaaacgtg
    33361 gcgaagaaat tgaattaacg caccgagaat ttgaattact tcaccattta gcgacacata
    33421 taggacaagt catgacacgc gaacatttat tggaaacggt ttggggatat gattattttg
    33481 gagatgtccg tacagttgat gtgacggtga gacgtttacg tgaaaagatt gaagatactc
    33541 ctggtcgtcc agaatatatt ttgactcgac gtggtgtagg ttattacatg aagtcttatg
    33601 aataatagtg ctgcaaatat taggtcgttt gagctagcct tacttttttt actagttttt
    33661 gttgctgtct attttgttta tctagcggtt agagatttta aaatgtctaa agatatccgg
    33721 cttttgaatt ggaaagtcag agatttgata gcaggaaatt attcagatag tattctaatt
    33781 caaggtgatg cagatttggt agaattaggg gaaagtctaa atgatttatc tgatgtcttc
    33841 cgaatggcac atgataactt agaacaagaa aagaatcgct tagcgagtat cttaacttat
    33901 atgactgacg gtgtgctggc tacagatcgt tcaggaaaaa tcgttatgat taatgagaca
    33961 gcccaacagc agttcaatct agcatatgat gaagctttat caatgaatat tgttgatatg
    34021 ttagggtcgg gaagtccata ttcgttccaa gatttagtct ctaagacgcc agaggtagtt
    34081 cttaatagac gtgatgaaaa tggtgaattt gttaccttac ggattcgatt tgctttgaac
    34141 cgtcgtgaaa gtggttttat ttccggttta gttgcagtat cacacgatgc tactgagcaa
    34201 gagaaagaag agcgtgaaag aagacttttt gtatctaatg tttctcatga acttcgtaca
    34261 cctttgacat ctgtaaaatc ttatttagaa gctttagatg agggagcgct caatgaagaa
    34321 gttgctccaa gcttcattaa ggtatctcta gatgaaacaa atcgtatgat gcgtatgata
    34381 tcagatttat taagtttatc gcgcattgat aatgaagtaa cgcatttaga tgttgaaatg
    34441 acgaatttta cagctttcat gacctcaatt ttgaatcgat ttgatcagat tagaaatcaa
    34501 aaaacagtca caggaaaagt ttatgaaatt gtcagagatt atcctcttaa gtcaatttgg
    34561 gtggaaattg atacagataa gatgactcaa gtgattgata acattttaaa taatgcagtc
    34621 aagtattcac cagatggtgg taagattaca gttaatctac gcacaactaa aacgcagatg
    34681 attttatcaa tatcagacca aggcttaggt attcccaaaa aagatttacc tctcattttt
    34741 gatcgttttt atcgtgttga taaggcgaga agtcgtcaac agggtgggac tggacttggt
    34801 ttgtcaattg caaaagaaat tgttaagcag cataagggat ttatttgggc taagagtgag
    34861 tatggtaaag ggtctacttt tacaatcgtc ttgccttatg ataaagatgc tgtaacttat
    34921 gaagaatggg aggacgttga agattaaaac atgtcagaaa taggatttaa atatagtatc
    34981 ttagcttcag gttcaactgg aaattgtttt tatattgaga caccccaaaa acgtctttta
    35041 attgacgctg gtttaacagg caagaaagtg actagccttc ttgcagaaat taatcgtaaa
    35101 ccagaagatt tagatgctat actagttacc catgaacatt ctgaccacat taaaggtgtt
    35161 ggcgtattgg ctaggaaata tcaccttgat atttatgcca atgagcagac gtggaaagtt
    35221 atggatgagc gtaacatgtt aggcaaagtt gatgttagtc aaaagcatgt ttttggacgc
    35281 ggtaaaacct taactttcgg ggatttagat attgagagct ttggtgtaag tcatgatgct
    35341 gtggatcccc aattttaccg tatgatgaaa gatgataaga gctttgtgat gttgacagat
    35401 acaggatatg ttagcgaccg aatggcaagc ttgattgaga atgcagatgg ttatttgatc
    35461 gagtcaaatc acgacattga gattctacgt tcgggttctt atccttggac acttaagcaa
    35521 cgtattttat cagataaagg acatttatct aatgaagatg gttcagaaac catgattcgc
    35581 actataggta accgtactaa acacatctat ttaggacact tgagtaagga aaataatatt
    35641 aaggaacttg ctcacatgac tatggaaaac aatttaatga gagctgactt tggtgtgggc
    35701 acagattttt ctgttcacga tacttcacct gattcagcta ccccactgac gaggatttaa
    35761 gattgaaaaa gattttttac attagtttag gatttattag tttagggata ggcattgcag
    35821 gtattgtctt gccagtagtt ccaacgactc cattagtatt gctttcagct ttttgctttt
    35881 cgagaagctc tgaaaaattt gatatttggt tgcgtcaaac taaagtttat aaatactacg
    35941 ctgcagattt tgttgaaagc agaagtattg ccccagcgcg taaaaagtca atgatttggc
    36001 aaatttatat cttgatgggg atttcaatat attttgctcc tctaatgtgg ctcaaactgg
    36061 gattattgat tggaactatt gtaggaactt atgtgctttt ttatgtagta cctgataaaa
    36121 ttgatgaata gttaacaaga gactaggtgt gtatcctagt cttttgttga atattcaaat
    36181 atgtaattaa ggaaacgttt tctttggaag ataagtttaa cttccaaaat gtttgattat
    36241 ttgtcaactg tggtataatg gtatttgttt aatattttat ctatgattga aatcaaggag
    36301 aaaaaatgaa agaattaaga tcaaagctgg aaaaagacta tggtattgtt tttgctaatc
    36361 aagaattatt agatacagct tttacccata catcatacgc aaatgagcat cgcctcctaa
    36421 acatttcaca taatgaacgt ttggaatttt taggagacgc tgttctacaa ttacttattt
    36481 cacagtatct ttttacaaag taccctcaaa aggcagaagg tgatttatca aaattacgtt
    36541 caatgattgt tcgtgaggaa agtcttgcag gtttttcacg tctatgtggc tttgatcact
    36601 atattaaact tggaaaagga gaagaaaaat ctggtggacg taatcgtgac actattttag
    36661 gagatttatt tgaggctttt ttaggagctt tattattgga taaaggtgtt gaagtggttc
    36721 atgcctttgt taacaaggta atgatccctc acgttgaaaa agggacctat gagcgtgtta
    36781 aagattacaa gacaagcttg caagaattac ttcaatctca tggggatgta aaaatagatt
    36841 atcaagttac taatgaaagt ggtccagcac atgcaaagga atttgaggtg actgtatctg
    36901 tcaaccaaga aaatttgagc caaggaattg gacgatcaaa gaaagctgct gaacaagacg
    36961 ctgccaaaaa tgctttagca acgttacaat gaggaaatca tgtttttaaa agaaattgaa
    37021 atgcaaggct ttaagtcctt tgcggataaa actaaggtag aatttgatca aggagttact
    37081 gctgttgtag gtccaaatgg atcaggtaaa agtaatatca cagagagttt acgttgggca
    37141 ttgggagaat caagcgcaaa aagtttacga ggaggtaaga tgccagacgt catatttgct
    37201 ggtacagaaa atcgtaagcc ccttaattat gcgcaagtga gtgttacttt agataacagc
    37261 gaccacttta tcgaaaatat agctgacgaa gttcgagttg aacgtcgtat ttttcgaaac
    37321 ggagatagtg aatatttaat agatggacgc aaagttcgct tacgtgatat tcatgattta
    37381 tttatggata ctggattagg gagagattct ttttctatta tttcccaagg acgcgttgaa
    37441 gctattttta atagtaaacc agaagagcgc cgtgccatat ttgaagaagc cgctggtgtt
    37501 ctgaaataca aaacacgtaa gaaagaaaca caatccaaat tagaacaaac tcaagggaac
    37561 ttagatcgct tagaggatat tatttatgaa ttggacatgc aagtccaacc attagaaaaa
    37621 caagcaagta ttgctaagcg ttttttagta ttagatgagg aacgacaagg actccactta
    37681 tctattttga ttgaagatat tttacaacat caatcagatt taactactgt tgaggaaaag
    37741 ctattgacag taagagaaga gttagcaact tattaccaac aacgtcagtc ccttgaagat
    37801 gaaaatcaat cgttaaaaca gaaacgtcac catttatctg aagaaattga ggttaaacag
    37861 ttagctcttt tagacgttac aaagttaaag tcagatttgg aacgccaaat tgatctgatt
    37921 cgtttagagt caagccaaaa ggctgaaaag aaagaagagg caggtcaacg cttagcagag
    37981 ttagaagtta aagctaaaga ttgtaatgac cagattaccc aaaaaaatat agagttaact
    38041 actctgtcgg aaaaaatagc ccaaataaga tcagaaattg ttagtataga aagttcctta
    38101 gagcgttttt caactaatcc ggatcaggtt attgaaaaac ttcgagaaga ctttgttacc
    38161 ttgatgcaag aagaagccga cacttcaaac gctttaacag ctttactagc tgatatagag
    38221 aatcaaaaac aagcttctca agctaaatct caagaaatac aagaagttag caaaaattta
    38281 gaagtattaa aaagtaacgc aaaggtagca ttggaacgat ttgaggctgc aaagaagaat
    38341 gttcgtcagt tattatctca ctaccaagat ttaggtcaaa ccttgcaaaa tctagaaggt
    38401 gagtataaga atcagcagtc aattttattt aatcatttag atgaaatcaa atcaaagcaa
    38461 gcacgtattt cgagtttaga atctattttg aaaaatcata gtaatttcta tgctggagtg
    38521 aagtcagttc tacaggctaa ggaccagctt ggaggtatca taggtgctgt tagtgagcat
    38581 cttagctttg acaaacatta ccaaacagca ttggagatag ctttgggagg tagcagccaa
    38641 cacatcattg ttgaagacga gagtgctgcc aaacgttcaa ttgctttttt aaagaaaaac
    38701 cgccaaggta gagcgacatt tctgccttta actacaataa aaccaagaga attagcacaa
    38761 cattatctct caaaattaca aagttctcaa gggtttttag gaatagctag tgaattggta
    38821 acctatgatc aacgcttgtc aaacattttt aaaaataact tagggttaac tgctattttt
    38881 gatacggttg ataatgctaa tgtagctgca agacaattga attatcaagt acgtttagta
    38941 accttagatg gaacagagtt acgccctgga gggtcgtact cagggggagc aaatcgtcaa
    39001 aataatactg tttttatcaa gcctgaattg gataatctaa aaaaagaatt gaaacaggca
    39061 caaagtaaac agttaattca agaaaaagaa gttgccgcat tgttagagca actcaaagag
    39121 aaacaagaaa ccttagcaca acttaagaat gatggagaac aagctcgtct tgaggaacaa
    39181 agagctgata tagagtatca acaactatca gaaaagcttg ctgatttaaa taaactatat
    39241 aatggcttgc aattaagcag cggggcgttg gaacaaacta cttctgaaaa tgaaaaaaat
    39301 cgtttagaaa aagaacttga acaatttgct attaaaaaag aagagttgac aacttcaatt
    39361 gctcaaatta aggaagataa agattcaatt caagaaaaag tcaataatct aacaacatta
    39421 ttgtcagagg cacagctaga agaacgtgat ttgctaaacg agcagaaatt tgaacgtgcc
    39481 aactgtacac gtctagagat taccttgtct gaaataaaac gtgacattag taatttacaa
    39541 acgttactaa gccatcagga cagtcaatta gacaaagaag agcttccacg tatagaaaaa
    39601 cagttgctac aagcaaataa gagaagagaa aatgacgaag aaaagcttgt gagtctccgt
    39661 tttgaattgg aggattgtga agctgcttta gatgatttag ctgcttctct ggctaaagaa
    39721 ggacaaaaaa atgaaagcct tattcgtcag caagctcaat tagagtcaca atgtgaacaa
    39781 ttatctcaac aattgatgat attctcacgt caattatcag aagactatca gatgacattg
    39841 gatgaagcga aggtaaaagc aaatgtcttg gaagatattt tgatggcgcg tgaacaattg
    39901 aaatctctac aagctaaaat taaagcttta ggacctgtta atattgatgc aattgctcaa
    39961 tttgaagaag ttcatgaacg attgaccttc cttaacacac agcgagacga cttagttcat
    40021 gccaaaaatt tattactaga gacgattaca gatatggatg atgaagttaa gactcgtttt
    40081 aaatcgacat ttgaagccat tcgtcatagt ttcaaagaaa cctttgtaca gatgtttggt
    40141 ggaggttctg cgaaccttat tctgacagaa ggtgatttat tatcagctgg ggttgatatt
    40201 tcggttcaac ctcctggtaa aaaaatccag tctttaaact tgatgtcagg tggtgaaaaa
    40261 gcattatcag cgttagcatt attatttgcc attatccgtg ttaagacaat tccatttgtc
    40321 atcttggatg aagttgaggc cgctttagat gaagcaaatg ttaagcgatt tggcgattat
    40381 cttaatcgat ttgataaatc aagtcaattt atcgttgtta ctcaccgaaa agggactatg
    40441 tctgctgctg atagtatcta tggggtaacg atgcaggaat caggcgtttc caaaattgtt
    40501 tctgtaaaat taaaagaagc tcaagaaatg accaattaga aaacagaagg ggatcccttc
    40561 tgttttttgg attttcagcc ttattttatg ttacaatagt aaatgtaaac cctttagtta
    40621 ggagattcca tgataaaatt agtagcaacc gatatggacg gtacattctt agatgaaaac
    40681 ggtacctatg ataaaaaacg tttagcaaat gtgctaaaaa aatttaaaga acaaggaatt
    40741 gtctttacgg cagcaagtgg gcgttcctta ttatcacttg aacaattatt tgctgacttt
    40801 cgtgatcaga tggcttttat agcagaaaac ggaagtgcag cagtcttatt taatcgcttg
    40861 gcatatgaac agcacttgag tcgagaacaa tacttagata ttattgacca tttgagtaag
    40921 agcccttata tggagaataa tgaatatgtc ttatctggca aggatggtgc atatattcta
    40981 agtgatgcca atccagatta tattgaattc atcactcatt attatgataa tttgcaaaaa
    41041 gtttctcact ttgaagatgt tgatgatatt gttttcaaag taacggctaa ctttactgaa
    41101 gagactgtta gacaagctga ggagtgggta aatcaagcta taccgtatgc cacagcagtg
    41161 acaacaggtt ttaaatccat tgatattatt ctaagctcag ttaataaacg aaatggttta
    41221 gagcacctct gtgaacaata cggtattaga gcagaagaag tcttatcttt tggtgacaat
    41281 attaatgatt tagaaatgct agagtggtca ggaaaagcta tcgcaacaga aaatgctaga
    41341 ccagaagtta aagagatagc agattgtatt ataggtcatc acaatgatca agctgtaatg
    41401 gcttatttag aaagtatggt ggattaatgt tggatattaa aatacttgcc ttagatttag
    41461 atggcacttt atttacaaca gataagaaag tttcagaaga aaataaagtg gccttaaaag
    41521 cagctagaga aaaaggtata aaagttgtta ttacaacagg gcgtccttta aaagcaattg
    41581 gtaatttgtt agaggaccta gagttagtat ctgatgaaga ttacagcatt acttttaacg
    41641 gtggtttagt tcaacaaaat acagggaaaa tcttagctaa aactgcaatg acacgacaag
    41701 aagtggaaga tatccatgag gagttgtatc aagtgggttt gccaacagat atcttaagtg
    41761 agggaacggt atatagtatt gctaacaagg gacaccattc tcaatatcat ttagccaacc
    41821 cactcttaga atttatagaa gttgatgatt tagagcaagt gccaaaggat gtagtttata
    41881 ataaaattgt tagtgttatt gatgctacat atcttgatca acaaattgct aaacttcctg
    41941 atagacttaa agttgactat gaaatgttta aatcacgtga tatcatttta gaattgatgc
    42001 ctaaaggtgt tcataaagca gtcggcttag aactactgac aaaacacctt gggttagata
    42061 gtagtcaagt tatggcaatg ggagatgaag caaatgattt atcaatgcta gagtgggcag
    42121 gcttaggagt tgcaatggct aatggtattc cagaagctaa ggcaacagca aaagcgacaa
    42181 ctatttgtaa taacgatgag tctggagttg ccgaagcagt tgggaaatat attttaagtg
    42241 aggaaaatta atgggattat ttgaccgcct atttggacat aagaaaaaag ataaagaact
    42301 agaaattgaa gcatcagaat ctgttgtttt agaaaatgaa gattcagtta ttgataaaga
    42361 ggaaagcagc aacttttcga aggagtcaac tttgaatcga acctctgaag tccctgtcgc
    42421 agaagatgat tctttcttag aattagaaag agatacagca ttgtcagaat cacatcaacc
    42481 agttacctct gaaattcatc ctttagagtc agaagatacc tctgaaattc ctgtaaaaga
    42541 agacgatagt tttttagaac tagaagatag ggctaagaca aaggtggcag atacttctga
    42601 ggtaggaaat gtagttcctg attccacgac tctttctgac aatgtttctg ctaaatctga
    42661 ggcatctttt agtgatgagg agcaatcaag tgatagtcag gcttcagagc agttttcaga
    42721 aactcccctt caagaagaga tgtctagtgg aaaaactgag gtacaaacag agtcagagga
    42781 tacatctgcg gctgatgctt ttttagcaga ttactatgct aaacgtaaag caattgagaa
    42841 agaaatctcg tctaattcgc tgtcaacaga tgagtcagaa ctttctgagg cacaggaagt
    42901 tttatcgcaa tcacaggcag acactataaa agctgagagt caagaggaaa aatataatcg
    42961 tagcctgaaa aaaacacgca caggttttag tgcacgcctt aatgctttcc tttctaactt
    43021 ccgtcgtgtt gacgaagagt tctttgaaga attagaagaa atgttaatct tatctgacgt
    43081 tggggtaaat gttgcaacac agttaactga agaccttcgt tatgaagcaa aattagagaa
    43141 tgccaagaaa tcagaggatt taaaacgtgt tatcgttgaa aaattagttg aaatctacga
    43201 aaaagatggt atttataatg aggctatcaa tttccaagaa ggtttaaccg taatgctttt
    43261 tgtaggtgtt aatggtgttg gaaaaacgac atctataggt aagttagctc atcaatacaa
    43321 gtctcaaggg aaaaaagtta tgttagtagc agctgatact ttccgtgcag gtgctgtggc
    43381 tcaactagta gaatggggac gccgtgttga tgtaccagtt gtcactggag aaaaaaaagc
    43441 agatccggct agcgttgtct ttgatggcat ggaaaaagcc gttgctcaag gcgttgatgt
    43501 cttgttaatt gatacagcag gacgtttgca aaataaagaa aaccttatgg cagagttgga
    43561 aaaaattgga cgtattatta agcgtgtcgt accagatgct ccccacgaaa ctttgcttgc
    43621 tttagatgca tctacaggac aaaatgcttt aagtcaagct aaagagttct cgaaaattac
    43681 accattaact ggccttattc tgactaaaat tgatggtact gctaagggtg gtgtggtact
    43741 ggctattcgt caagaactag atattcctgt taaattcatt ggtttcggcg aaaaaatcga
    43801 tgatattggt gaatttaatt cagaagattt catgcgtggc cttctggaag gaattttata
    43861 agaaaaaaga actttcattt agaaagttct ttttatagta cgacgggcat gtcgtacatc
    43921 tgaggtgcaa gtcctctgtg ggcacccgct accggtgaac ccaatagcga ctcccaagct
    43981 tgactatcgt gaggtagcgg ggagaggaag ggatagcgaa atcatggctc ta
//
LOCUS       DATRUK010000011        43506 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.011, whole genome shotgun sequence.
ACCESSION   DATRUK010000011 DATRUK010000000
VERSION     DATRUK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43506
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.011"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..998)
                     /locus_tag="VBE34_000522"
     CDS             complement(<1..998)
                     /locus_tag="VBE34_000522"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO7263263.1"
                     /translation="MYLIEPIRNGKRITDGAIALAMQVYILQNVFLDDDILFPYYCDP
                     KVEIGKFQNAVIETNQEYLKEHDIPVVRRDTGGGAVYVDSGAVNICYLMKDHGQFGDF
                     KRAYEPAIKALKTLGASSVEMRGRNDLVIDGKKVSGAAMTIVNGRIYGGYSLLLDVDF
                     DAMEKVLNPNRKKIESKGIKSVRSRVGDIRSHLSEDYRHITTDQFKDLMVCQLLHIDH
                     IDQAKRYHLTEKDWAAIDALADEKYKNWDWNYGNSPQYSYHRDARFPSGTYDFHLEIE
                     KGIITNCRIYGDFFSSKDISDIENLLIGCPMKEELVLEKLSTLSLEDYFGQTSPEE"
     gene            complement(1025..1903)
                     /locus_tag="VBE34_000523"
     CDS             complement(1025..1903)
                     /locus_tag="VBE34_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEO7263264.1"
                     /translation="MTVWKTLEKTNHSQSEILSQLIEESDAIVVGIGAGMSAADGFTY
                     IGPRFEEAFPDFIAKYQLLDMLQASLYDFEDWEEYWAFQSRFVALNYLDQPVGQAYLD
                     LKDILAKKEYHIITTNADNAFAVADYNLEKVFHIQGEYGLWQCSQHCHQQTYRNDQAI
                     RQMIAQQKDMKIPSNLIPKCPKCDQPFEINKRNEEKGMVEDADFHAQRQRYENFLSQH
                     QNDKVLYLEIGVGHTTPQFIKHPFWRFVSLNENSLFVTLNHKHYRIPQKIRSRSVQLT
                     QHIAELIAEAKKEIIG"
     gene            complement(1872..2690)
                     /locus_tag="VBE34_000524"
     CDS             complement(1872..2690)
                     /locus_tag="VBE34_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEO7263265.1"
                     /translation="MPNQKQLLLAMIEYLQSEKLTDVDDLRTTDLQTVWRGLVNQQDP
                     QNISQEYLSLEDRYLSHWWNTQKVKTIDVCHQTVYSNVFTYHGDICLLQVDAIVNAAN
                     SKLLGCFIPNHHCIDNQIHTFAGSRLRLACHQLMTQQGRMEAVGQAKLTESYHLPCKY
                     VIHTVGPYVKVDQKPSRIREDLLKSSYKSCLQLAAQANLKTIVFPCISTGEFGFPNQR
                     AAELAVQEILEWQRENQHKLYIIFNTFTPKDQEIYQKLLLKEEIDDSVEDSRKN"
     gene            complement(2683..3015)
                     /locus_tag="VBE34_000525"
     CDS             complement(2683..3015)
                     /locus_tag="VBE34_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEO7263266.1"
                     /translation="MKKIANYLLIEKNEELYTISLTPELQDDLGTVGYVEFTDDTNLE
                     VDDVILNIEASKTVMAILSPLTGKVVKVNTAASQEPTLLNSEKADENWLVVLTDVDYA
                     AFEALENA"
     gene            complement(3044..4030)
                     /locus_tag="VBE34_000526"
     CDS             complement(3044..4030)
                     /locus_tag="VBE34_000526"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HEO7263267.1"
                     /translation="MKLSVLDYGLIDYGKTASDAIQETILLSQEAEQLGYHQFWVAEH
                     HGVKAFSISNPELMIMHLANQTKSIKIGSGGIMPLHYSSFKLAETLKTLETCHPNRVS
                     IGLGNSLGTVKVSNALRSLHKAHDYEEVLEELKSWLIDESSSKEPLVQPTLSSFPDLY
                     VLGSGQKSAYLAAKLGLGFTFGVFPFMDKDPLTEAKKLSSLYYHQFEEYYPNKSPNLM
                     VAPFVVIADTSEEAENIAKTLDIWMLGNKDFNEFATFPTIEEANHYQLTPEQKAKIKS
                     NRHRMIVGDPKQVKESLDALVNASQAEELLLIPLVPGLDQRIKSLKLLSQLY"
     gene            complement(4027..5226)
                     /locus_tag="VBE34_000527"
     CDS             complement(4027..5226)
                     /locus_tag="VBE34_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEO7263268.1"
                     /translation="MNNVQGNLFRPLTLPNGLSLENRFVLSPMVTNSSTSEGFVTDDD
                     IAYAVRRAKSAPLQITGAAYITEYGQLFEYGFSVSKDEDIPGLTKLAKAMKSKGAKAV
                     LQLTHAGRFSSHTLARHGYVYGPSPMQLQSPYPHQVKELTHKDILRIIDEYVQATRRA
                     IQAGFDGVEISSAQRLLIQTFFSTFSNQRKDEYGPQTLTNRCRLGLEVFKAVQKVIRE
                     EAESDFILGFRATPEETRGSQIGYSIEEFMEFLEKILAIAQVDYLAIASWGHDVFRNT
                     IRSEGVYKGQLVNQVIFEHFGDRVPIMATGGINSASKVFEALQHAHMIGASTPLVVDP
                     EFLQKIKAKRSDQINLRIKVNDLEGLAIPKASFKDIVPLMDYGESLPKEAREVFRELR
                     SNYREER"
     gene            complement(5219..6055)
                     /locus_tag="VBE34_000528"
     CDS             complement(5219..6055)
                     /locus_tag="VBE34_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEO7263269.1"
                     /translation="MEWQDLAQLPVSIFKDYVTDAQDAEKPFIWTEVFLREINRSNQE
                     IILHIWPMTKTVILGMLDRELPHLELAKKEIISRGYEPVVRNFGGLAVVADEGILNFS
                     LVIPDVFERKLSISDGYLIMVDFIRSIFSDFYQPIEHFEVETSYCPGKFDLSINGKKF
                     AGLAQRRIKNGIAVSIYLSVCGDQKGRSQMISDFYKIGLGDTGSPIAYPNVDPEIMAN
                     LSDLLDCPMTVEDVIDRMLISLKQVGFNDRLLMIRPDLVAEFDRFQAKSMANKGMVSR
                     DE"
     gene            6194..6892
                     /gene="coaB"
                     /locus_tag="VBE34_000529"
     CDS             6194..6892
                     /gene="coaB"
                     /locus_tag="VBE34_000529"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEO7263270.1"
                     /translation="MYNMAMKILITSGGTTEKIDTVRSITNHATGALGKIIAEKYLRE
                     GHQVTLVTTKNAVKPESATNLSIFEIEDVDSLIKTLKPLVKEHDILIHSMAVSDYTPV
                     YMADFEKVKSSDDLDTFLRKDNHEGKISSESEYQVLFLKKTPKVISLVKKWNPQITLV
                     GFKLLVNVTKENLFKVARHSLIKNKATFILANDLIDITSKHHIAYLLDHDNVYKATTK
                     EDIAQLIYEKVKKI"
     gene            6889..7431
                     /gene="coaC"
                     /locus_tag="VBE34_000530"
     CDS             6889..7431
                     /gene="coaC"
                     /locus_tag="VBE34_000530"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEO7263271.1"
                     /translation="MIKRITLAVTGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNPIHLDVMDEHNPKIINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALAMPDETPKLIAPAMNTKMYHNTITQRNIDILKKIGYQEIEPRISLLACGDT
                     GQGALADISTILKCIQEVAN"
     gene            7477..8049
                     /locus_tag="VBE34_000531"
     CDS             7477..8049
                     /locus_tag="VBE34_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7263272.1"
                     /translation="MKKKNKSSNIAIIAIFFAIMLVIHFLSSFIFSFWLVPIKPTLMH
                     IPVIIASIAYGPRIGATLGALMGGISVANSSIVLLPTSYLFSPFVENGNFYSLIIALV
                     PRILIGIIPYFVYKLLHNRFGLAISGAIGSLTNTVFVLSGIFIFFSSTYNGNIKLMLA
                     GIISSNSLAEMVIAAIIVYLTVPRILNIKH"
     gene            8159..9877
                     /locus_tag="VBE34_000532"
     CDS             8159..9877
                     /locus_tag="VBE34_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO7263273.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSSGLIQVIGEDVDIEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVNADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQIATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATVGNDLADAETKIANIEKE
                     ITTFVN"
     gene            complement(9941..10138)
                     /locus_tag="VBE34_000533"
     CDS             complement(9941..10138)
                     /locus_tag="VBE34_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263274.1"
                     /translation="MKKRLQLLLLIFISAVSWFISNQLATKNTVLSVLIGLVAVIGIT
                     WLIVSLLYYLIVKLIAVIFHD"
     gene            complement(10152..11885)
                     /locus_tag="VBE34_000534"
     CDS             complement(10152..11885)
                     /locus_tag="VBE34_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7263275.1"
                     /translation="MKASHPKKIQKKLVQDLLSKKSLVGMALLGTVVQVCLTVYLPVL
                     IGQAVDVVLSPHSMILLLPIMWKMVAVILANTIIQWINPLLYNRLIFHYVASLRKAVM
                     EKLSLLPIAYLDKRGIGDLISRVTTDTEQLSNGLLMVFNQFFVGLLTILVTIFSMAKI
                     DLLMLFLVLFLTPLSLFLARFIAKKSYHLYQNQTASRGRQTQFIEEMVSQESLIQAFS
                     AQEESSDHFRTINQEYANFSQSAIFYSSTVNPSTRFINSLIYGFLAGIGALRIMSGAF
                     SVGQLITFLNYVNQYTKPFNDISSVLSEMQSALACAERLYSILEESSPNITGTEKLDS
                     STVKGQIDFKNVIFGYNKSKPLLNGINLHIPAGAKVAIVGPTGAGKSTLINLLMRFYE
                     VDGGNILLDGKPITDYEPSQLRQEIGMVLQETWLKSATIHDNIAYANPKASREEVIEA
                     AKAANADFFIKQLPNGYDTYLEDAGDSLSQGQCQLLTIARIFLKLPRILILDEATSSI
                     DTRTEVLVQEAFQMLMKGRTSFIIAHRLSTIQTADIILVMVSGEIVEVGNHSELIAQK
                     GIYYQMQNAQK"
     gene            complement(11886..13607)
                     /locus_tag="VBE34_000535"
     CDS             complement(11886..13607)
                     /locus_tag="VBE34_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7263276.1"
                     /translation="MKRLTYYFKGYIKETIFGPLFKLLEASFELLVPIVIAKMIDETI
                     PRGDRSSLLLQIGLIFFLAAVGVVVAITAQYYSSKAAVGYTRQLTEDLYQKVMSLGKK
                     DRDELGTASLITRLTADTFHIQTGLNQFLRLFLRAPIIVFGAIIMAFSISPSLTIWFL
                     IMVVTLFIIVFVMSRLLNPIYLKIRTSTDYLVKLTRQQLQGVRVIRAFNQVDRESEAF
                     NDINYHYTNLQLKAGRLSSLVTPLTFLVVNITLVVIIWRGNLNIANHLLSQGMLVALI
                     NYLLQILVELLKMTMLVTSLNQSYISAKRIIAVFERPSEIIDDKLEPKYSNKALEVQE
                     MAFSYPNSSEKALSDITFSMNVGETLGIIGGTGSGKSTLVNLLLHIYKVQEGDIDIYH
                     QGKSPDTISNWRSLVRVVPQNAQLFKGTIRSNLSLGLGKVSEEKLWTALEIAQASDFV
                     KEKDGQLDAPVESFGRNFSGGQRQRLTIARALVQDKIPFLILDDATSALDYLTEARLF
                     KAIIKHFNQTNLIIVSQRINSIQNADRILLLDKGKQVGFDNHQSLLAHNKVYKSIYHS
                     QNFKEEE"
     gene            complement(13619..14221)
                     /locus_tag="VBE34_000536"
     CDS             complement(13619..14221)
                     /locus_tag="VBE34_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEO7263277.1"
                     /translation="MFKFLKRLIALIIIIFIGYRLVIIHENVKKVLQYHDLVQNTLAE
                     NGSEANVHLVLSMIYTETKGDAIDVMQSSESISGTTNSITDSHTSIKHGVTLLSQNIS
                     QAKKAKVDVWTAVQAYNFGSSYIDYVADHGGENSIELAKNYSKNVVAPSLGNYNGDTY
                     FYYHPLALISGGKLYKNGGNIYYSREVQFNLYLIKIMELF"
     gene            complement(14223..15200)
                     /locus_tag="VBE34_000537"
     CDS             complement(14223..15200)
                     /locus_tag="VBE34_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEO7263278.1"
                     /translation="MMDFYIKQIIIHQFSPNDTELVLSDTPLTLTPRIDDYFRKKLSK
                     VFSDEAKRGYFGEDNVFMSHLQDDLYVSSCQIAQLWKEEFVISEDQKTNDLVFIQFDK
                     DGMEHFAFLRISLKEHFAHVSENQEQPITITQNNLPSAAQTPDEALVVNKSSKQYYLI
                     EKRIKHNGSFANYFSENLLQVQPEQSVKKSIKMVEQTAQKIAENFNKDDFSFQSKMKS
                     AIYKNLEEEQELSPEKLADQLFDDNLTARLTFVDELKDAIPGSVQVSDIDHSRQIKKL
                     ENQKLSLSNGIELIVPNNVYQDAESVEFIQNPDGTYSILIKNIQDIQNK"
     gene            complement(15205..16461)
                     /locus_tag="VBE34_000538"
     CDS             complement(15205..16461)
                     /locus_tag="VBE34_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEO7263279.1"
                     /translation="MIFDKDNFKEFDQELWQAIHDEEIRQQNNIELIASENVVSKAVM
                     AAQGSVLTNKYAEGYPSHRYYGGTDCVDVVESLAIERAKMLFNAEFANVQPHSGSQAN
                     AAAYMALIEPGDTVLGMDLAAGGHLTHGASVSFSGKTYHFVSYSVDPKTEMLDYDNIL
                     KIAQETQPKLIVAGASAYSRIIDFEKFRQIADAVDAYLMVDMAHIAGLVASGHHPSPI
                     PYAHVTTTTTHKTLRGPRGGLILTNDEAIAKKINSAVFPGLQGGPLEHVIAAKAVALK
                     EALDPSFKIYGEDIIKNAQAMAKVFKEDDDFHLISDGTDNHLFLVDVTKVIENGKKAQ
                     NVLEEVNITLNKNSIPFERLSPFKTSGIRIGTPAITSRGMGVEESRRIAELMIKALKN
                     HENQDVLTEVRQEIKSLTDAFPLYEN"
     gene            complement(16553..17149)
                     /locus_tag="VBE34_000539"
     CDS             complement(16553..17149)
                     /locus_tag="VBE34_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEO7263280.1"
                     /translation="MNDLGQILEDHGAVIMPTETVYGIFAKALSEEAVNHVYELKKRP
                     RDKAMNLNICDFETILKYSKNQPTYLKQLYDAFLPGPLTIILEASQEVPDWINSGLSS
                     VGFRMPKHPVTLDMIANHGPLIGPSANISGCDSGRVFSEIQKQFNHQVLGIEDDKALT
                     GVDSTIIDLSGDRVKILRQGAITQEVLTATIPELIFEN"
     gene            complement(17142..17972)
                     /gene="prmC"
                     /locus_tag="VBE34_000540"
     CDS             complement(17142..17972)
                     /gene="prmC"
                     /locus_tag="VBE34_000540"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEO7263281.1"
                     /translation="MNYAQLIKHYEQLLEACGEEVENFIYVLKDLKQWSTTDYLLNQN
                     SSVSDTDQQLMENIFQQLKKHRSPQYITGKAYFRDLIFFVDERVLIPRPETEELVDLI
                     LSENKVEDCSVLDIGTGSGAIAISLKKERPSWDVLASDISVSALDLAKENANNCDAEV
                     TFIESDVFSNISDKFDIIVSNPPYISYNDKDEVGKNVLASEPHSALFADEEGLAIYRK
                     IIENSREYLQPRGKLYFEIGYKQGDDLRSLLKRYFPNNRCRVLKDIFGKDRMVVLDNE
                     "
     gene            complement(17972..19051)
                     /gene="prfA"
                     /locus_tag="VBE34_000541"
     CDS             complement(17972..19051)
                     /gene="prfA"
                     /locus_tag="VBE34_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEO7263282.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQNISDAEEMIKDASGDAELEEMAKEELKESKAAKEEYEERLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYEI
                     DQKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTQKLEALNK"
     gene            complement(19086..19655)
                     /locus_tag="VBE34_000542"
     CDS             complement(19086..19655)
                     /locus_tag="VBE34_000542"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEO7263283.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     EFGVVSSRIGMRREAVPISDDMDIFSYIQNLPQKPYCVLIDECQFLSKKNVYDLARVV
                     DDLDVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPDI"
     gene            19793..19975
                     /locus_tag="VBE34_000543"
     CDS             19793..19975
                     /locus_tag="VBE34_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEO7263284.1"
                     /translation="MPFVKIDLFEGRSQEQKNELAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGELKKK"
     gene            20124..21062
                     /locus_tag="VBE34_000544"
     CDS             20124..21062
                     /locus_tag="VBE34_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEO7263285.1"
                     /translation="MQLSHSLRLMGTTIDIQINSKNAQKQIQEVIELLELYKNRFSAN
                     DFNSELMAINNNAGIKPIQVHPDLFELITIGKEHSLARPSNLNIAIGPLVQTWRIGFS
                     DAKLPSPSEVSEAMILSDPTHILLDSKNQSVFLNQIGMKIDLGALAKGYIADKIMTYL
                     KNEMIDSAIINLGGNVLVHGDNPNRSEGYWVIGIQHPKKKRGQNIGAVKIKNQSVVTS
                     GTYERRLIIDDKEYHHIFDRQTGYPIQTEMASISIVSKQSVDCEIWTTRLFGLSIKEA
                     LDILNAVSYIEGIIITKDDRIYLSDGLKHHFQLFYH"
     gene            21080..21682
                     /locus_tag="VBE34_000545"
     CDS             21080..21682
                     /locus_tag="VBE34_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7263286.1"
                     /translation="MKLIGIVGTNSNKSTNRQLLQYMQQHFADKAEIELIEVKDLPLF
                     NKPADKNVPQVILDIAAKIEEADGVIIGTPEYDHSIPSALMSVLAWLSYGIYPLLNKP
                     VMITGASYGTLGSSRAQLQLRQILNAPELKASVLPDEFLLSHSLQAFDKDGNLHDIET
                     SQKLDAIFDDFRLFVKITGKLSNARDLLQKEAENFDWESL"
     gene            21706..22941
                     /locus_tag="VBE34_000546"
     CDS             21706..22941
                     /locus_tag="VBE34_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7263287.1"
                     /translation="MKFVGIVGSNAEQSYNRMLLEFIRKNFKTKFELEVLEIDDIPMF
                     NQVQNWEESFQLRLLNNKITRADGVIIATPEHNHTITAALKSVLEWLSFAVHPLENKP
                     VMIVGASYYDQGTSRAQIHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDDNGNIINPG
                     TVQFLETCLDNFIKYVGVVSSLRKPKAIESEDLYCQGQIDTTITSIDPDDPDWLDKAA
                     EKVNAVEGDAYVKLDNGLLTVNQLNMFLNAMPFELTYADDNNQFLYYNNNHPDPDTML
                     GKRVREQVGNRMATVHGSLPPARMKNVEWVIGTLRNGNQEYVRTVVPGTPPEVINTHN
                     YQAMYYPDGSYAGINEIIFNFKPWLDWYLQTTGQRLVGGTTAAPGASAVDATSSASTS
                     TEDAPVDQATDATSGASEH"
     gene            23040..23828
                     /locus_tag="VBE34_000547"
     CDS             23040..23828
                     /locus_tag="VBE34_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEO7263288.1"
                     /translation="MSLFQEKIAYNCAKKEALYKESLGRYALRSMLAGAYLTMSTAAG
                     IVAADTIGKISPALSGFVFAFIFSFGLIYVLIFNGELATSNMLYLTAGAYNKNISWKK
                     AITILIYCTFFNLVGACILAWLFNQSYSFQHLTNDSFLGHVVAKKLGKPSSGAFLEGI
                     IANMFVNLAILAYMLLKEESAKMTVILSAIFMFVFLSNEHLIANFASFMLAAFSHIEH
                     IKGFTLLNVIRQWTLVFFGNWIGGGVFIGLAYAWLNKTKTSHHE"
     gene            complement(23927..25201)
                     /locus_tag="VBE34_000548"
     CDS             complement(23927..25201)
                     /locus_tag="VBE34_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEO7263289.1"
                     /translation="MKFNEQSNSQAALLGLQHLLAMYAGSILVPIMIASALGYNAKQL
                     TYLIATDIFMCGIATLLQLRLSKHFGVGLPVVLGCAFQSVAPLSIIGAQQGSGYMFGA
                     LIASGIYVVLVAGIFSKVANFFPPIVTGSVITTIGLTLIPVAMGNMGDNAKEPSLQSL
                     TLSLVTIGVVLLINIFAKGFLKSISILIGLISGTILAAFMGLVDASVVAEAPLVHIPK
                     PFYFGAPRFEFTSILMMCIIATVSMVESTGVYLALSDITNDKLDSKRLRNGYRSEGLA
                     VLLSGLFNTFPYTGFSQNVGLVQISGIRTRKPIYFTALFLVILGLLPKFGAMAQMIPS
                     PVLGGAMLVLFGMVALQGMKMLNQVDFEHNEHNFIIAAVSIAAGVGFNETNLFISLPN
                     TLQMFLTNGIVISTLTAVVLNIILNGLPKKLI"
     gene            complement(25201..25782)
                     /locus_tag="VBE34_000549"
     CDS             complement(25201..25782)
                     /locus_tag="VBE34_000549"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEO7263290.1"
                     /translation="MKLLEERILKDGDVLGENILKVDSFLTHQVNFELMQEIGKVFAD
                     KYKEAGITKVVTIEASGIAPAVYAAQALGVPMIFAKKAKNITMTEGILTAEVYSFTKQ
                     VTSQVSIVSRFLSNDDTVLIIDDFLANGQAAKGLLEIIGQAEAKVAGIGIVIEKSFQD
                     GRDLLEKTGVPVTSLARIKAFENGRVVFAEADA"
     regulatory      complement(25886..25981)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(26037..27020)
                     /gene="guaC"
                     /locus_tag="VBE34_000550"
     CDS             complement(26037..27020)
                     /gene="guaC"
                     /locus_tag="VBE34_000550"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEO7263291.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIISSRSQADTSVKLGNYTFKLPVI
                     PANMQTIIDEEVAETLACEGYFYIMHRFNEEARRPFIKRMHDKGLIASISVGVKDYEY
                     DFVTSLKEDAPEFITIDIAHGHSNSVIEMIQHIKQELPGTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCSKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHLESPGKLVEVEGQQFKEYYGSASEYQKGEHKNVEGKKIL
                     LPVKGRLEDTLTEMQQDLQSSISYAGGKELDSLRHVDYVIVKNSIWNGDSI"
     gene            complement(27224..28564)
                     /locus_tag="VBE34_000551"
     CDS             complement(27224..28564)
                     /locus_tag="VBE34_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7263292.1"
                     /translation="MTKKGWRIMPAIITTAILGFSGILIETSMNVTFPLLMKEFGVNP
                     AVIQWVTTGNLLAVAVTVPLSAFMIKNLSERQIFTLANVLFLFGVLIDSFAPNLAILL
                     VGRVLQGVGTGLALPLLFHIILTQIPMERRGLMMGVAAMVTLLAPAVGPTYGGVISGM
                     LGWKMIFMLLAPILIISTFIGLASIPKRQVRINDKLNFPAFVSLGIGLATLLLAIEKM
                     SIFYLLVAIVSFVIFYYLNKQLEFLNLNVFKDKDFSILLYGVLAFQMIPLALSFLLPN
                     LLQLVLHQTSTKAGLFMFPGAIAVVFLSPFAGYLLDKIGAFKPIMIGISLSLIGLIGT
                     AIFIPAKFVVVLLAFDILTKIGMGIGASNMVTTALTKLKPAQSADGNSILNTLQQFAG
                     AFATAVASQIFTIGQVAIPKNGAIIGSQFAVLFVIVVVILAIVGLTYLRKRKAI"
     gene            28856..29527
                     /locus_tag="VBE34_000552"
     CDS             28856..29527
                     /locus_tag="VBE34_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263293.1"
                     /translation="MAFASNESENNFFITLKTYFNYLFSIQIITDISTLNHADFDGSF
                     AFHDIETSIPHLVIDSNYLAISQTNSKTEANDIKTFSELSKTMTEFYYMLNFDLFNHL
                     PYRFRLHNKDGQTIYSNHKPEDPFDIYPEEEYPIDKWVQNSLIEKKAKELHLLLPGAS
                     QDYILVQSYKRLENDSGQLVGYIEHVHNIKPLLEGYLKESGQAIVGWSDVTSGASISN
                     DDFEL"
     gene            complement(29598..31598)
                     /locus_tag="VBE34_000553"
     CDS             complement(29598..31598)
                     /locus_tag="VBE34_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEO7263294.1"
                     /translation="MQHVNHSSFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GISSVTESFILGSISLIIWTLTLITTIKYVLVALKADNHHEGGIFSLYTLVRKMTPWL
                     IVPAVIGGATLLSDGALTPAVTVTSAVEGLKVVPSLQHIFQNQSNVIFATLFILLLLF
                     AIQRFGTGVIGKLFGPIMFIWFAFLGISGLLNSFAHPEVFKAINPYYGLKLLFSPENH
                     KGIFILGSIFLATTGAEALYSDLGHVGRGNIHVSWPFVKVAIILSYCGQGAWILANKN
                     AGNELNPFFASIPSQFTMHVVILATLAAIIASQALISGSFTLVSEAMRLKIFPQFRST
                     YPGDNIGQTYIPVINWFLFAITTSIVLLFKTSAHMEAAYGLAITITMLMTTILLSFFL
                     IQKGVKRGLVLLMMIFFGILEGIFFLASAVKFMHGGYVVVIIAVAIIFIMTIWYKGSK
                     IVSRYVKLLDLKDYIGQLDKLRHDHRYPIYHTNVVYLTNRMEGDMIDKSIMYSILDKR
                     PKKAQVYWFVNIKVTDEPYTAEYKVDMMGTDFIVKVELYLGFKMRQTVSRYLRTIVEE
                     LLESGRLPKQGKTYSVRPDSKVGDFRFIVLDERFSSSQNLKPGERFVMLMKSSVKHWT
                     ATPIRWFGLQFSEVTTEVVPLIFTANRGLPIKEKIELTTTGD"
     gene            complement(31733..32494)
                     /locus_tag="VBE34_000554"
                     /pseudo
     CDS             complement(31733..32494)
                     /locus_tag="VBE34_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649058.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(32556..33548)
                     /gene="pta"
                     /locus_tag="VBE34_000555"
     CDS             complement(32556..33548)
                     /gene="pta"
                     /locus_tag="VBE34_000555"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEO7263295.1"
                     /translation="MRSLFGGLREKVASDYVKIVFPEGSDERVLRAAARLKFEGLVDP
                     VILGKADEVHDSLARLGFVDQDYSVIDPEQYEKFEEMKEAFVEIRKGKATMEDADRLL
                     KDVNYFGVMLVKLGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRENT
                     QERYIFADCAINIDPNAQELAEIAVNTADTAKIFDINPKIAMLSFSTKGSAKAPQAEK
                     VQEAAKIAKDLSPELAVDGELQFDAAFVPETAEIKAPNSDVAGKANVFIFPDLQSGNI
                     GYKIAQRLGMFEAIGPILQGLNAPVNDLSRGSSAEDIYKLAIITAAQAIDEKSK"
     gene            complement(33574..34458)
                     /locus_tag="VBE34_000556"
     CDS             complement(33574..34458)
                     /locus_tag="VBE34_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO7263296.1"
                     /translation="MKFEYIAEERCKVKTLLKSHDVSRGLLAKIKYRGGKIFVNGEEQ
                     NAIFLLEIGDVVTIDIPDEPSHETLEPVPHDLDIVYEDDHFLILNKPFGFASIPSSIH
                     SNTIANFIKHYYVSNNYANQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQAKAIE
                     KRYYALVSGSGDLADSGDIIAPIARDVDSIITRRVHESGKYAHTSYQVVARYGDVRLV
                     DIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRMDLGINRQALHCHSLSFYDPFMGKIN
                     KQTLDLTDDFDSVIMELH"
     gene            complement(34455..35291)
                     /locus_tag="VBE34_000557"
     CDS             complement(34455..35291)
                     /locus_tag="VBE34_000557"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEO7263297.1"
                     /translation="MTQMNFTDRATRVAIIANGKYQSKRVASKLFAAFKHDPDFYLSK
                     KDPDIVISIGGDGMLLSAFHMYEKQLDKVRFVGVHTGHLGFYTDYRDFEVDTLINNLK
                     NDKGEQISYPILKVTITLEDGRVIRARALNESTIKRIEKTMVADVVINQVVFERFRGD
                     GILVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVYRTLGSSVIIPKKDAIE
                     IVPKRVGVYTISIDNKTVHYKNVTKIEYSIDEKSINFVSTPSHTSFWERVNDAFIGEP
                     EH"
     gene            complement(35266..35937)
                     /locus_tag="VBE34_000558"
     CDS             complement(35266..35937)
                     /locus_tag="VBE34_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO7263298.1"
                     /translation="MSMDWETFLDPYIQTVGELKIKLRGIRKQFRKQNRHSPIEFVTG
                     RVKSVESIQEKMVLRGISEENLAQDLQDIAGLRIMVQFVDDVDEVLALLRKRHDMTVV
                     QERDYITHMKSSGYRSYHVVVEYPVDTIDGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPEEIKQRLEKTAKIALELDEEMRKIREDIREAQLLFDPLNRKLSDGVGNSDDT
                     DEFYR"
     gene            36047..36619
                     /locus_tag="VBE34_000559"
     CDS             36047..36619
                     /locus_tag="VBE34_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEO7263299.1"
                     /translation="MTHLEIEYKTLLNKDEFNRLTSLFSHVQPITQTNYYFDTETFEM
                     KAHRMSLRIRTLPNRAELTLKIPREVGNLEHNHDLTLEEAKYIVKNGQFPEDTEIASL
                     ILEKGVDPTKLAVFGQLTTTRREMETSIGLMALDSNIYADIKDYELELEVKQPKQGKR
                     DFDQFLKENNINFKYAKSKVARFSATLKNN"
     gene            36796..37770
                     /locus_tag="VBE34_000560"
     CDS             36796..37770
                     /locus_tag="VBE34_000560"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO7263300.1"
                     /translation="MAEQYADKQIKLFSLTANREIAEKISQASGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVVPYFGYSR
                     QDRIAASREPITAKLVANMLVKAGVDRVLTLDLHAVQVQGFFDIPVDNLFTVPLFAEH
                     YNQLGLSGEDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREEGYIIGEVEG
                     KKAIIIDDILNTGKTFAEAAKILERGGATEIYAVASHGLFAGGAADILESAPIREIIV
                     TDSVLSKERIPSNIKYLTASHLIADAIIRIHERKPLSPLFSYRSDKKD"
     gene            37774..38889
                     /locus_tag="VBE34_000561"
     CDS             37774..38889
                     /locus_tag="VBE34_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO7263301.1"
                     /translation="MIYLDNAATTALTPSVIEKMTNVMTSNYGNPSSIHTFGRQANQL
                     LRECRQIIAEYLNVNSREIIFTSGGTESNNTAIKGYALANQLKGKHIITSEIEHHSVL
                     HTMTYLSERFGFDITYLKPNHGQITAKDVQEALRDDTIMVSLMFANNETGDFLPIQEI
                     GQLLRNHQAVFHVDAVQVFSKMELDPHSLGIDFLAASAHKFHGPKGVGILYCAPHHFD
                     SLLHGGDQEEKRRASTENIIGIAGMSQALTDATTNTLKNWTHISQLRTTFLDAISDLD
                     FYLNNGQDCLPHVLNIGFPRQNNGLLLTQLDLAGFAVSTGSACTAGTVEPSHVLTSLY
                     GANSPRLNESIRISFSELNTQEEILELAKTLRKIIGD"
     gene            38891..39238
                     /locus_tag="VBE34_000562"
     CDS             38891..39238
                     /locus_tag="VBE34_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEO7263302.1"
                     /translation="MAFEKEINLPDCNYIYTISPNIKKYTLRDTTFTQNRLGNYELER
                     LLETVPNSGDGFPLKITINKELSGFKLLITDKSGLRIVNIFKSTDHKILQDKFYFLMD
                     SLVERNIFTKTAK"
     gene            39393..40031
                     /locus_tag="VBE34_000563"
     CDS             39393..40031
                     /locus_tag="VBE34_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEO7263303.1"
                     /translation="MIMDKSIPKATAKRLSLYYRIFKRFNTDGIEKASSKQIADALGI
                     DSATVRRDFSYFGELGRRGFGYDVKKLMNFFAEILNDHSTTNVMLVGCGNIGRALLHY
                     RFHDRNKMQISMAFDLDSNDLVGKTTEDGIPVYGISTINDHLIDSDIETAILTVPSTE
                     AQEVADILVKAGIKGILSFSPVHLTLPKDIIVQYVDLTSELQTLLYFMNQQR"
     gene            complement(40044..40724)
                     /gene="radC"
                     /locus_tag="VBE34_000564"
     CDS             complement(40044..40724)
                     /gene="radC"
                     /locus_tag="VBE34_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEO7263304.1"
                     /translation="MYHIELKKEALLPRERLVDLGADRLSNQELLAILLRTGIKEKPV
                     LEISTQILENISSLADFGQLSLQELQSIKGIGQVKSVEIKAMLELAKRIHKAEYDRKE
                     QILSSEQLARKMMLELGDKKQEHLVAIYMDTQNRIIEQRTIFIGTVRRSVAEPREILH
                     YACKNMATSLIIIHNHPSGSPNPSESDLSFTKKIKRSCDHLGIVCLDHIIVGKNKYYS
                     FREEADIL"
     gene            complement(40820..41953)
                     /locus_tag="VBE34_000565"
     CDS             complement(40820..41953)
                     /locus_tag="VBE34_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO7263305.1"
                     /translation="MKFEKRQVYYVVITFAICYAIQAYWGAVSNILTTLHKAIFPFLM
                     GAGIAYIINIVMSVYERLYIKLFKGSRLLMAIKRSVSMILSYATFIGLIVWLFSIVIP
                     DLISSLSSLLVIDTGALAKLVNNLNENKQISEALNYMGTDKDLVSTLSGYSQQILKQV
                     LSVLTNLLTSVSSIAATLLNVFVSFIFSIYVLANKEQLGRQFNLLIDTYLGSTGKTFH
                     YVRHILHQRFHGFFVSQTLEAMILGSLTVIGMLIFQFPYALTVGVLVAFTALIPVVGA
                     YIGVTIGFILIATESLTEAFLFVLFLILLQQFEGNVIYPKVVGGSIGLPSMWVLMAIT
                     IGGALWGILGMLLAVPVAATIYQIVKDHIIKRQTLRNRARTYR"
     gene            complement(42044..43483)
                     /locus_tag="VBE34_000566"
     CDS             complement(42044..43483)
                     /locus_tag="VBE34_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEO7263306.1"
                     /translation="MMVKQVFPKGFLWGGATAANQCEGAYNVDGRGLANVDVVPTGED
                     RFAIISGQKKMFDFEEGYFYPAKESIDFYHHYKEDLALLAEMGFKTYRMSIAWTRIFP
                     KGDELYPNEAGLRFYENIFKECRKYGIEPLVTITHFDCPIYLIKHYGGWRSHKMIGFY
                     ERLVRALFTRFKGLVKYWLTFNEINMILHAPFMGAGLYFEDGENQEQIKYQAAHHELV
                     ASAIAVKIAHEVDPNSQIGCMLAAGQYYPNTCHPQDYWASMQKNRENYFFIDVQARGK
                     YPNYAKKYFEHLGISIQMTAEDLALLRDYTVDFISFSYYSSRVASGNPTVSEQVQENI
                     FASLKNPYLKSSEWGWQIDPLGLRITLNAIWDRYQKPMFIVENGLGAVDIPDENGYVE
                     DDYRIDYLRQHIAAMRDAIYVDGVNLIGYTTWGCIDLVSAGTGEMEKRYGFIYVDRNN
                     KGEGTLKRYKKKSFYWYKKVIASNGSQIE"
BASE COUNT        14347 a         8375 c         6848 g        13936 t
ORIGIN      
        1 atttcttctg gactagtctg accaaaataa tcttctaatg ataaagtact taatttttcc
       61 aagactaatt cttctttcat agggcaacca atgagaaggt tttcaatatc tgaaatatct
      121 ttagaactga aaaagtcacc ataaatacgg caattcgtaa taataccttt ctcaatttct
      181 aagtgaaaat cgtaagttcc tgacggaaaa cgagcatctc tgtgatacga atactgtggt
      241 gagttaccat aattccaatc ccaattctta tatttttcat cagctagagc gtcaatcgca
      301 gcccaatctt tttcggttag atgatagcgt tttgcctgat caatatgatc aatatgtaat
      361 aattgacaaa ccattaaatc tttaaattga tcggttgtga tatgacgata atcttctgac
      421 aagtggctac gaatatctcc tactcgactt cggacagatt ttatcccttt agattcaatt
      481 tttttccgat taggatttaa cactttttcc atagcatcaa aatcaacatc taataaaaga
      541 gagtaaccac cgtaaatacg accgtttact atcgtcattg ctgcacctga tactttttta
      601 ccatcaataa ctaagtcatt tcgtcccctc atttcaacac tagaagcacc taaagttttt
      661 agggccttaa tagctggctc ataagctcgt ttgaaatcac caaattgtcc atgatctttc
      721 attagatagc aaatatttac agcacctgag tcaacgtaaa cagctcctcc accagtatct
      781 cgacgaacaa caggaatatc atgttctttc aaatattctt gatttgtctc tataactgca
      841 ttctgaaatt ttccgatttc aactttagga tcacaatagt atggaaataa aatatcatca
      901 tctaaaaaaa cattttgtaa aatataaact tgcatggcaa gtgcaatcgc accgtctgtg
      961 atacgcttac catttctaat tggttcaata agatacattt aggtctcctt ttatagcaat
     1021 ttagttatcc aataatttct tttttcgctt cagcaattaa ttcagcgata tgctgtgtca
     1081 gttgcacact ccttgagcga atcttttgag gaatgcgata atgcttgtga ttaagagtaa
     1141 caaataaaga gttttcattt aaagagacga atcgccaaaa cggatgctta ataaattgtg
     1201 gcgtggtatg tccaactcca atctctagat aaagaacttt gtcattttga tgttgtgata
     1261 agaaattttc ataacgttgt cgttgagcat gaaagtcagc atcttccacc ataccttttt
     1321 cttcgttacg tttattaatt tcaaacggtt gatcacactt tgggcatttg gggattaagt
     1381 tactaggaat cttcatatcc ttttgttgtg caatcatttg acgaattgcc tgatcatttc
     1441 gataagtttg ttgatgacaa tgttgactac actgccataa gccatattca ccttgaatat
     1501 gaaaaacttt ttctaaatta taatcagcca cagcgaaggc attatcagca ttagttgtaa
     1561 taatatgata ttcttttttc gctaaaatat cctttaaatc aagataagct tgtcctaccg
     1621 gttgatctaa ataattaagt gccacaaaac gactttggaa ggcccaatat tcttcccaat
     1681 cttcaaagtc ataaagactt gcttgcaaca tatctaatag ttgatattta gcaataaaat
     1741 ccggaaaagc ttcttcaaat ctggggccaa tataggtaaa tccatccgct gcagacatgc
     1801 ctgcaccaat accaactaca atagcatctg attcttctat taactgactt agtatttctg
     1861 attgagaatg attagttttt tctagagtct tccacactgt catctatttc ctcttttaat
     1921 aataattttt gataaatctc ttggtcttta ggtgtgaatg tattgaaaat aatatagagt
     1981 ttgtgctgat tttcgcgttg ccactctaat atctcttgaa cagccaattc agcggctctt
     2041 tgattaggaa aaccaaattc tcccgttgat atacaaggga agacaattgt tttaagatta
     2101 gcttgagctg ccaattgtaa gcaggattta taagaagact ttaataaatc ctctcttata
     2161 cgagatggct tttgatcaac ttttacataa ggtccgactg tatgaataac atacttacaa
     2221 ggaagatgat aggattcggt aagtttagct tgtcctacgg cttccattcg tccttgttgt
     2281 gtcatcaatt gatgacatgc taaacgtaat cgtgaacctg caaacgtatg aatctgattg
     2341 tcaatacaat ggtgattagg aataaaacaa cctaacaact tactatttgc tgcattaaca
     2401 atagcatcca cttgtaataa gcaaatatcc ccatggtagg taaatacatt tgaatatacc
     2461 gtttgatgac agacgtctat cgtttttact ttttgagtat tccaccaatg tgacaaatac
     2521 ctatcttcta aagacaggta ctcttgacta atgttttgag gatcttgttg attaactaga
     2581 ccacgccaaa ccgtttgcaa atcggtggtc ctcaagtcat caacgtctgt taatttttct
     2641 gactgaagat actcaatcat ggctagcaat agttgttttt gattaggcat tttctaaagc
     2701 ttcaaaagca gcataatcaa catctgttaa cacaaccaac caattttcgt cagctttttc
     2761 agaatttaac aaagttggtt cttgacttgc agctgtatta actttaacaa ctttacctgt
     2821 aagaggagat aaaattgcca taaccgtctt tgaagcttca atatttaaaa taacatcatc
     2881 aacttctaaa tttgtatcat ctgtgaattc cacatatcca acagttccca agtcatcttg
     2941 aagttcggga gttaagctga tagtataaag ttcttcattt ttttcaatta ataagtagtt
     3001 cgcaattttt ttcatgagta atttcctttc tgagaaaacg tgattaatat aactgtgata
     3061 ataattttaa ggatttgatt cgttgatcaa gtcctggaac aagaggaatc aaaagtagtt
     3121 cttctgcttg actagcatta actaaagcgt caaggctttc tttaacttgt tttggatcac
     3181 ctacaatcat acggtggcga tttgatttaa tttttgcttt ttgttcagga gtcaattggt
     3241 aatgattagc ttcttcaata gtaggaaatg ttgcaaattc attgaagtct ttatttccta
     3301 gcatccagat atctaaagtc tttgctatgt tttcagcttc ttctgaagta tcagctataa
     3361 ctacaaatgg agctaccatc aaattaggag acttattagg ataatattct tcaaactgat
     3421 gataataaag agatgaaagc tttttagctt ctgtcaatgg gtctttgtcc ataaaaggaa
     3481 aaacaccgaa ggtaaagcct aagccaagtt tagccgctaa ataagctgat ttttgaccac
     3541 tccccaacac atataagtct gggaagctag aaagagtcgg ttgaactaat ggttccttac
     3601 tggatgattc atcaataagc catgacttca attcctccag tacctcttcg taatcatgtg
     3661 ctttatgtaa gctacgaagt gcatttgaaa ctttaactgt ccctagtgaa tttcctaaac
     3721 caatacttac tcgattcgga tgacatgtct ctaatgtctt gagagtctcc gcgagtttaa
     3781 aactactata gtgcagaggc attatacctc cagagccaat tttgatagat ttagtctggt
     3841 tagccaaatg cattatcatt aattctggat tgctaatact gaatgcctta acaccgtgat
     3901 gttcagccac ccaaaattga tgatagccta gttgctccgc ctcttgtgat aaaagaatcg
     3961 tttcttgtat tgcatcactt gcagtttttc cataatcaat aagcccataa tcaaggacac
     4021 ttaatttcat ctttcctccc tataattgct tcgaagttct ctaaaaactt ctcgagcttc
     4081 ctttggtaaa gattctccat aatccatcaa aggaacaata tctttaaagg aggcttttgg
     4141 aatcgccaaa ccttctaaat cattcacttt aatccttaaa ttaatctgat cagaacgctt
     4201 agcttttatt ttttgtaaaa attccggatc aactactaag ggcgtggaag ctcctatcat
     4261 atgagcatgt tgtaaggctt caaacacctt tgaagcacta ttaatacctc cagttgccat
     4321 aattggaact ctatcaccaa aatgttcaaa tataacttga ttaaccagct gccctttgta
     4381 aactccctca gaacgtatag tatttctaaa gacatcatgc ccccagcttg caatggcaag
     4441 ataatctacc tgagcaatcg ctaaaatttt ttcaagaaat tccataaact cttcaatgct
     4501 atatccaatt tgtgaaccgc gagtttcttc aggcgtcgct ctaaaaccaa ggataaaatc
     4561 tgactccgct tcttctctga taactttttg taccgcttta aaaacttcta agcctaaacg
     4621 acagcgatta gtcaaagttt gaggaccata ctcatctttt ctttgatttg agaaggttga
     4681 gaaaaaagtt tgaattagta atcgttgtgc agaagatatt tcaacaccat caaatccagc
     4741 ttgtatagct cgtcttgtag cctggacata ttcatcgata atccttagta tatctttgtg
     4801 tgttagttct ttaacctgat gtgggtatgg cgattgcaat tgcatagggc tgggaccata
     4861 tacatatccg tgtcttgcca aagtgtgact tgagaaacgt ccagcatgcg tcagctgcaa
     4921 aacagctttc gctcccttag acttcattgc tttagccaat ttagtcaaac caggaatgtc
     4981 ctcatccttt gaaacgctaa aaccatattc aaataactga ccatattctg tgatatatgc
     5041 tgctccagta atttgtaatg gagcggattt agcacgacga acagcatatg ctatgtcgtc
     5101 atcagttaca aaaccttctg atgtagaaga attagttacc ataggtgata gaacaaaacg
     5161 attttctaag ctcaatccat tgggaagtgt taatggacga aataaattac cttggacatt
     5221 attcatctct gctcaccatc cccttattag ccatagactt agcctgaaat ctatcaaact
     5281 ctgcaactaa atcgggtcta atcatcagta aacgatcatt aaaacctact tgtttaaggc
     5341 taatcaacat acgatcaata acatcttcta ctgtcatagg acaatctaat agatcagata
     5401 gattagccat aatttcagga tctacatttg gataagcaat tggactaccc gtatcaccta
     5461 gaccaatctt ataaaaatct gaaatcattt gactccgccc tttttgatcg ccacaaacgc
     5521 taaggtaaat tgataccgca ataccattct ttatacggcg ctgagccaag ccagcaaatt
     5581 ttttgccatt tatactaaga tcaaacttac caggacaata ggaggtctct acttcaaagt
     5641 gctcaatagg ttgataaaaa tccgaaaata tacttctaat aaaatcgacc attataagat
     5701 acccatctga gatagacaat tttctctcaa aaacatctgg aataaccaat gaaaaattta
     5761 aaattccttc atcagctaca actgcgagac ctccaaaatt ccgaacaact ggttcataac
     5821 cacgactgat gatttctttt ttagctaatt ctaaatgtgg taattctcga tctaacatcc
     5881 ccagaatgac tgtcttagtc atcggccaaa tatgcaaaat aatttcttga tttgagcgat
     5941 taatctccct taaaaatact tctgtccata taaaaggttt ttccgcgtct tgagcatctg
     6001 taacatagtc tttaaaaata gatacaggta actgcgctag atcttgccac tccaataaat
     6061 ctgacctcct ttctctagaa aactctgcaa gatttatttt atcgcaatcg cttgaaattt
     6121 tccagtaagt ttccctcaaa ttccattact atcaagaaga aatatatctt ttatttacga
     6181 aaagcatttt tttatgtaca atatggctat gaaaattttg attacatcag gtggaactac
     6241 cgagaaaatt gataccgtta ggagtatcac gaatcacgca acaggagctt taggaaaaat
     6301 aattgcagaa aaatatttac gagaaggaca tcaggtgaca ctcgtgacta ctaaaaatgc
     6361 agtcaaacca gaatctgcta ccaacttatc catatttgag attgaggacg ttgactcatt
     6421 aataaaaacc ttgaagccat tagtgaaaga gcatgatatt ttaattcata gcatggcagt
     6481 gtctgactat acccccgtct acatggctga tttcgaaaag gttaaatcaa gcgatgattt
     6541 agatactttt ttgcgcaaag ataaccacga agggaaaata tcatctgaat cagaatatca
     6601 agtccttttt ctcaaaaaaa caccaaaagt tatcagcctt gtgaaaaaat ggaatcctca
     6661 aattactcta gtaggtttta aactacttgt gaacgttact aaagaaaacc tttttaaagt
     6721 tgctcgtcat agcttaatca aaaataaagc tacgtttatt ttagctaatg acctcattga
     6781 tattacctca aaacatcaca tagcctacct acttgatcat gataatgtgt ataaagctac
     6841 caccaaagaa gatattgctc aattaattta cgagaaggta aaaaaaatat gattaaacgt
     6901 attacactag ccgtcacagg ttcaatttcg gcctataaag cagcagacct tacaagtcaa
     6961 ttaactaaaa ttggctatga tgttcatatc ataatgacac aagctgccac acaatttatt
     7021 acaccgttaa ctctgcaagt attatcaaaa aatcctattc atttagatgt tatggatgag
     7081 cataacccaa aaatcatcaa ccatatcgaa ttagcaaaac gcacagattt atttatcgtt
     7141 gcacctgcct ctgctaatac tattgctcat ctagcatatg gcttcgctga taacatcgta
     7201 acaagcgttg ctcttgctat gcccgatgaa actccaaaac ttatcgcacc agcaatgaat
     7261 acaaagatgt atcataatac aatcacccaa cgtaacattg atattttgaa aaaaataggt
     7321 tatcaggaaa ttgaacctcg tattagtttg cttgcctgtg gtgataccgg tcaaggagcc
     7381 ctagctgata tctctactat acttaaatgt atacaagaag ttgcaaatta ggctgtaaat
     7441 ttgtatacta tttctagttt aatttaagga gttataatga aaaagaaaaa caaatcctct
     7501 aacattgcta taattgcaat cttttttgct attatgcttg tcattcattt tttgtcatca
     7561 tttattttta gtttttggtt agtccctatt aaacctactt tgatgcatat cccagttatt
     7621 attgcatcta tagcctatgg acctcgtatt ggtgcaactc taggcgcctt aatggggggg
     7681 atcagcgtag ctaacagcag cattgttcta ttaccaacga gttacctctt ctcacctttt
     7741 gttgaaaatg gtaattttta ttcgctaatt attgcacttg taccacgtat tctaatcggg
     7801 attattcctt atttcgttta caaattacta cacaaccgct ttggtttggc tatctcaggt
     7861 gctataggct ctctaacaaa cacagtattt gttttatctg gaatttttat ctttttttca
     7921 agtacttata atgggaatat caagctaatg ctcgctggga ttatttcatc taattcatta
     7981 gctgagatgg tcattgcagc tatcattgta tatctaactg ttcctcgtat tctcaatatt
     8041 aaacattaaa aactggccag gaagaaatct tcctggcttt tcttacttat attttatagt
     8101 catattgaaa aaaacatgtt ataatgaaag cgtttataaa tattgaagga gtatttcaat
     8161 gacttacaca gaaaattttc aaaaatggtt agattttgag cagcttcctg actatcttcg
     8221 tcaagaatta ctatcaatgg atgaaaaaac aaaagaagat gctttttaca caaacttaga
     8281 atttggtact gctggtatgc gtggttatat tggtgctggt acaaatcgta ttaatattta
     8341 tgttgtccgt caagcaaccg aaggattggc aaaattaatc gaaactaagg gagaggaagc
     8401 taaaaaacgt ggtgtagcca ttgcttatga ttcacgccac ttctctccag aatttgcttt
     8461 tgagtctgca caggtcttgg cacagcatgg aattaaatct tacgtatttg aagctcttcg
     8521 tccaacacca gaactctctt ttgctgttcg ccatttaaat gcctatgctg gtattatggt
     8581 tactgcaagc cacaatccag cacctttcaa tggttataaa gtctatggac aagacggagg
     8641 tcaactacca ccagctgatg ctgatgcact aacagacttt atccgtgcta ttgaaaatcc
     8701 atttgcagtt gaacttgctg atttagatga aagcaaatca tcgggcctta ttcaggttat
     8761 cggtgaagat gttgatattg aatatcttag agaggttaaa gatgttaata tcaatcaaga
     8821 tttaatcaat aactttggaa aagacatgaa aattgtctat actcctctcc acggaactgg
     8881 tgagatgttg acacgccgtg cccttgctca agccggtttt gaatctgttg ttgtagtaga
     8941 atcacaagct aaggcagatc cagacttttc tactgttaaa tcgcctaacc ctgaaagcca
     9001 agctgccttt gctcttgctg aagaactcgg acgagaagtt aatgcagatg tcttagtagc
     9061 tactgatcct gatgcagacc gcctaggcgt tgaaattcgc caaccagatg gttcttataa
     9121 aaacctttct ggaaatcaaa ttggagcgat tattgcgaaa tacatcctgg aagctcataa
     9181 aacagcagga acacttccag aaaatgctgc tctcgctaaa tcaatcgtat caacagaatt
     9241 agtaacaaaa attgccgaaa gttatggcgc tacaatgttt aacgttttaa ctggattcaa
     9301 attcatcgct gagaaaattc aagagtttga agaaaaacat aaccacacct atatgtttgg
     9361 ttttgaagag agcttcggat atcttattaa accatttgtc cgtgataaag acgctattca
     9421 agcagttctt ttggttgctg agattgcagc ttattaccgt tcgcgtggtt taacacttgc
     9481 agatggtatt gatgaaattt acaaagaata tggttatttt gctgaaaaaa cgatttcagt
     9541 aacactttca ggagtggatg gagctgctga aattaagaag attatggaca aattccgtga
     9601 aaatggtcca aaacaattta acaatactga tattgtatta ttagaagatt tccaaaaaca
     9661 aatcgctact aaaaatgatg gaacaatatc taacttaaca actcctccaa gtaatgtttt
     9721 gaagtacacg ttagctgatg attcatggat tgcagttcgc ccttcaggaa ctgaaccaaa
     9781 aatcaagttc tatattgcta cggtcggtaa tgatttagca gatgcagaaa caaaaatcgc
     9841 caatattgaa aaagagatta caacatttgt aaattaataa aaaaagctag gaacttactc
     9901 ctagcttttt ttatatctat ctaacgatgt tctattattt tcaatcatga aatataactg
     9961 caattaactt cacgatcaaa taatagagca gacttacaat taaccacgtt atcccaataa
    10021 ctgcaactaa acctattaaa acagataata ccgtattctt agttgctagt tggttactga
    10081 taaaccatga aactgcagaa ataaatatca gtaataatag ttgaagcctt tttttcatat
    10141 aattcctctt actacttttg tgcattttgc atttgataat aaataccttt ttgagctatt
    10201 aattcagagt ggttacccac ttctacaatt tcaccagata ccataactaa aataatatct
    10261 gctgtttgaa tggtagataa gcgatgtgca ataataaagc ttgttctccc cttcatcaac
    10321 atttggaatg cttcttgaac aagaacttca gtacgagtat ctatagacga tgtcgcctca
    10381 tctaatatca atattctagg taatttaaga aaaatacgag caattgtcaa tagctgacat
    10441 tgcccctgtg ataacgaatc tccagcatct tctaaatacg tatcatatcc attcggtaat
    10501 tgtttgataa agaaatctgc attagctgct tttgcagcct ctatcacttc ttcacggcta
    10561 gctttaggat tggcatatgc aatgttatcg tgaatcgtag cactttttaa ccacgtttct
    10621 tgtaatacca ttccaatttc ttgccttaat tgtgatggct cataatcagt aataggctta
    10681 ccatctaaaa ggatatttcc cccgtcaact tcatagaaac gcattaataa attgattaaa
    10741 gtagacttcc cagcacctgt tggtcctaca atagcgacct tagctcctgc tggaatgtgt
    10801 aaattaatac cattcaatag tggttttgat ttattataac caaaaataac gtttttaaaa
    10861 tcgatttgac ctttgaccgt tgatgaatca agtttttctg tacctgttat atttggtgac
    10921 gattcttcta aaatagagta aaggcgttct gcacaagcca gagcactttg catctcagac
    10981 aggacggaag agatgtcatt aaatggctta gtatattgat tgacataatt taagaaagtg
    11041 attaattgac caacactaaa agcacctgac ataatacgaa gggcaccaat ccctgctaag
    11101 aagccataaa tcaaactatt gataaatcgg gttgatggat tgacagttga agaataaaaa
    11161 attgctgatt gagaaaagtt agcatactct tgattaatag tcctaaaatg atcactactt
    11221 tcttcttgcg cactgaatgc ttgaataagg ctttcttgac tcaccatctc ttcaatgaat
    11281 tgagtttgtc gtcctcgtga tgccgtttga ttttgatata agtgataact ctttttagca
    11341 ataaagcggg ctagaaagag tgataaaggc gtcagaaata ataccaaaaa aagcattaat
    11401 aaatcaatct ttgccatact aaaaatagta actagaatag ttaataaacc aacaaagaac
    11461 tggttaaaaa ccattaataa accatttgat aattgctccg tatcggtagt cacacgacta
    11521 atcaagtcac ctatgccacg tttatcaagg taagctatag gtaatagact caacttttcc
    11581 attacagcct ttcttaaaga agcaacataa tgaaaaatta agcggttata gagcagagga
    11641 ttaatccatt gaataatagt atttgctaaa ataactgcaa ccatcttcca cattataggt
    11701 aacaataata tcatagaatg aggcgataat acaacgtcta ccgcctgtcc aattagaaca
    11761 ggcaaataaa cagtgaggca aacttgtact accgtcccta ataatgccat tcctactaag
    11821 ctttttttag aaagaagatc ttgaacaagc tttttttgta tctttttagg atgacttgct
    11881 ttcatttact cctcctcctt aaaattttgt gaatggtaaa tggacttata aactttgtta
    11941 tgagctaata aagattgatg attatcaaaa ccaacttgtt tgcccttatc aaggagtaag
    12001 attctatctg cattttgtat actattaatt ctctgtgata caataataag attagtttgg
    12061 ttgaaatgtt taattatagc tttaaataaa cgagcttctg ttaaataatc caatgcagat
    12121 gttgcatcgt ctaaaatcaa aaatggaatc ttatcttgaa ctaaagctct tgcaatcgtc
    12181 aacctttgcc tttgaccacc agagaaattc ctaccaaaac tctctacagg ggcatcaagt
    12241 tgaccatctt tttcttttac aaaatcacta gcttgtgcta tttctaaagc agtccaaagt
    12301 ttttcctcac taactttacc aagtcccaaa gaaaggttag aacgaatagt tcctttaaac
    12361 aactgagcat tttgaggaac aactcttacc aggctacgcc aatttgaaat tgtatctgga
    12421 ctttttccct gatgatatat atcaatatcc ccttcttgca ctttataaat atgaagcagt
    12481 aaattaacca aggttgattt cccagagcca gttccaccaa ttatccctaa agtttctcct
    12541 acattcatag aaaaagtaat atcagataaa gctttttcag aggagtttgg ataagaaaat
    12601 gccatttctt gcacttctaa agccttattc gaatattttg gctcaagttt gtcatcaatt
    12661 atttcagacg gtctttcaaa aacggctata attcgcttag cactgatata actttggttc
    12721 agtgatgtca ccaacattgt catttttaac aattcaacaa gaatttgcaa taagtaatta
    12781 attaaagcaa ccaacattcc ttgtgataaa agatgattag ctatatttaa attaccacgc
    12841 caaataatca caaccaaagt gatattaaca actagaaatg ttagaggcgt tactaaacta
    12901 gaaagcctac ctgcttttaa ttgtaaattc gtatagtgat aattgatatc attaaatgct
    12961 tcactctctc tatctacttg attaaaggca cggataactc gtacaccttg aagttgttgc
    13021 ctagtcaact ttaccaaata atcggttgaa gttctgattt taaggtaaat aggatttaat
    13081 agtcgagaca ttacaaaaac aatgataaat aacgtgacaa ccattatcaa gaaccaaata
    13141 gtcaatgagg gactaatgga aaaagccata attatggcac caaaaacaat aatcggagcc
    13201 ctcaaaaata aacgtaaaaa ttgatttaaa ccagtttgga tgtgaaatgt atcagcagtc
    13261 aaacgagtaa tcaaactagc agttcccaat tcatctctgt cttttttacc cagtgacatg
    13321 actttttgat agagatcctc tgtcaattgt ctcgtataac caactgcggc ttttgaagaa
    13381 taatattgag cagttatcgc tactacaaca cccaccgcag ccaaaaagaa aatcaatcca
    13441 atttgcaaca gtaaactact tctatcccct cgtggaatgg tctcgtcaat catttttgca
    13501 ataacgattg gtaccaaaag ttcaaaagag gcttctaata atttgaaaag gggtccaaag
    13561 atagtttctt tgatataccc cttaaaataa taagttagtc ttttcatact tacccttctt
    13621 aaaacaactc cattattttt atcaaataaa ggttgaattg aacttcacgt gagtagtaaa
    13681 tattaccacc atttttatag agcttccctc ctgaaattaa ggcgagagga tgatagtaaa
    13741 agtaagtgtc cccgttataa tttcctaggc tgggagctac aacattttta gagtaatttt
    13801 tggccaactc aatggaattt tcacccccat gatccgctac ataatcaata taagaagaac
    13861 caaaattata tgcttggaca gcagtccata catcaacctt agctttttta gcttgactta
    13921 tattttgtga caacagagtg acgccatgtt taatactggt gtgactatcc gtaatactgt
    13981 tcgtagttcc actgatactt tcacttgatt gcatcacatc tatagcatct cccttagttt
    14041 cagtataaat catagataat actaagtgca cattagcctc tgaaccattc tctgcaagag
    14101 tattctgcac taaatcatga tattgaagca cttttttaac attttcatgg atgataacta
    14161 gacggtaccc aatgaaaata ataataatta atgctatcaa acgttttaaa aatttaaaca
    14221 tattatttat tttgaatatc ctgtatgttt ttgattagta ttgagtatgt accatcaggg
    14281 ttttggataa attcaactga ttcggcatct tggtagacat tgttgggtac aatcaattca
    14341 ataccatttg aaagagatag cttctgattc tctaacttct taatttgacg ggaatgatca
    14401 atatcactta cttgaacaga tccaggtatt gcgtctttca attcatcaac aaaagtcagc
    14461 ctggctgtta aattatcatc aaataattga tctgctaatt tttctggaga caattcttgc
    14521 tcttcttcta aattcttata aatagccgat ttcatttttg attggaaaga aaaatcatct
    14581 ttgttgaaat tttcagcaat tttttgagct gtctgttcaa ccatttttat agatttttta
    14641 acagactgtt ctggttgtac ttgtaataaa ttttcagaaa aataattagc aaatgaacca
    14701 ttgtgtttga tacgcttttc aattaaatag tattgctttg atgacttgtt aacaaccaaa
    14761 gcttcatctg gagtttgagc agcacttgga aggttattct gtgttattgt aattggctgc
    14821 tcttgatttt cagaaacatg agcaaaatgt tcctttaatg agattcgaag gaaagcaaaa
    14881 tgttccattc catccttgtc aaattgaata aaaactaagt cattcgtttt ttgatcttcc
    14941 gatatgacaa actcttcttt ccaaagttgc gcaatttgac aggaagaaac atataaatca
    15001 tcttgtaaat gactcataaa tacattatcc tctccaaaat agccacgctt agcttcatca
    15061 gaaaaaactt tacttaattt tttacgaaag tagtcatcaa tccttggagt tagggttaac
    15121 ggagtatctg atagcactaa ctctgtgtcg ttaggagaaa actgatgtat aataatctgt
    15181 ttgatataaa aatccatcat aacattaatt ctcatacaaa gggaaagcgt ccgttaatga
    15241 tttaatttct tgacgaactt ctgtgaggac atcttgattt tcatgatttt tgagtgcctt
    15301 aatcattaat tcagcgattc ttcgagattc ttcaactccc attcctcgac tagtaattgc
    15361 tggagtacca attcgaatac ctgatgtttt aaatggtgat agtcgctcaa acggtataga
    15421 gttcttgtta agggtgatat tgacttcttc aagaacattt tgtgcctttt tgccattctc
    15481 aataactttt gtgacgtcta ctaagaacaa atgattatca gtaccatcag aaatcaaatg
    15541 aaagtcatca tcttctttaa aaactttagc catggcttga gcatttttaa taatatcctc
    15601 accgtatatt ttaaaggaag gatctagagc ttctttcaaa gctacagctt ttgcagcaat
    15661 tacatgttct aatggtccgc cttggagtcc tggaaagact gcagaattaa tttttttagc
    15721 aattgcttca tcattggtta aaatcaaacc acctctaggt ccacgtagag tcttatgtgt
    15781 tgtcgttgtc gttacatgag cataaggaat aggactagga tgatgaccag aagcaactaa
    15841 acctgcaatg tgagccatgt caaccattaa gtaagcatct actgcgtctg ctatttgacg
    15901 aaacttttca aaatcaataa tgcgagaata ggccgacgcc cctgcaacaa ttaatttagg
    15961 ttgcgtttcc tgtgctattt tcaaaatgtt atcatagtct agcatctccg tcttaggatc
    16021 aacagagtaa gaaacaaaat gataagtttt tcctgagaag ctaactgaag cgccatgtgt
    16081 taagtgccca cctgctgcta agtccatccc taagacagta tctcctggct cgattaaagc
    16141 catgtaagct gctgcattag cctgactacc agaatgtggt tgaacattag caaactcagc
    16201 attaaataac atcttagctc gttctatagc taaactttca actacatcaa cacaatctgt
    16261 tcctccataa taacgatgac taggataccc ttcggcatat ttatttgtca aaacagaacc
    16321 ttgtgctgcc ataacagctt ttgaaacaac gttttctgat gcaattaatt caatattatt
    16381 ttgttggcga atttcctcat catgaatagc ctgccaaagt tcttggtcaa attctttaaa
    16441 attgtcttta tcaaaaatca ttttttactc cttaagctcg ttataacttt taaataatgg
    16501 tttcaatcat tatatttatc acatttttac tattagttgc aagatgatta aattaatttt
    16561 caaaaattaa ttcaggtatc gtagccgtta gtacctcttg cgtgattgca ccttgcctta
    16621 aaatcttaac tctatcacca gacaaatcaa taatagtaga atccacacct gtcaatgctt
    16681 tatcatcttc aatacctaaa acttgatgat taaactgttt ttgaatttct gagaatacac
    16741 gaccactatc acatccagaa atattagcag aaggaccaat caatggacca tggttagcaa
    16801 tcatatcaag ggttacaggg tgcttaggca tccgaaatcc tacgcttgat aacccagaat
    16861 tgatccaatc cggaacttct tgtgaagctt ccaaaataat ggttagcggt cctggtaaaa
    16921 aagcatcgta caattgcttc aaataggtcg gttgattttt agaatatttt aaaatagtct
    16981 caaaatcaca tatatttaag ttcatagcct tatcacgagg tcttttttta agctcataga
    17041 cgtggttcac agcttcttca ctcaaagctt tggcaaagat tccgtagacc gtttctgttg
    17101 gcataataac tgcgccatga tcctctaaaa tctgtcccaa atcattcatt atctaatacc
    17161 accattctat ctttaccaaa tatatctttc aaaactctgc aacgattgtt tggaaaatat
    17221 cttttcaata aacttcgtaa atcatcacct tgtttgtaac ctatttcaaa atataattta
    17281 cctctaggtt gcaaatattc tcttgagttt tcaatgattt tacgataaat agctaatcct
    17341 tcttcgtctg caaaaagggc actatgaggc tctgaagcta gcacattctt ccccacctcg
    17401 tctttgtcat tgtaagaaat atagggagga ttagacacaa taatatcaaa tttatctgaa
    17461 atgttactaa agacatctga ttctataaaa gtcacttccg catcacaatt attagcattt
    17521 tcctttgcca agtctaatgc agatacagaa atgtcactag ctagaacatc ccaagagggg
    17581 cgttcttttt ttagagaaat ggcaattgct ccactacctg taccaatatc taaaacagaa
    17641 caatcttcta ctttattctc tgataagatt agatcaacca actcctctgt ctcaggcctc
    17701 ggaattaaaa ctcgttcatc aacaaagaaa ataagatctc taaagtaagc tttacctgta
    17761 atatactgcg gtgatctatg ttttttaagc tgttgaaaaa tattttccat taattgctga
    17821 tcagtatcac tcacactact attttgattt aaaaggtaat cagttgttga ccattgtttt
    17881 aggtctttta atacataaat aaaattttcg acttcttcac cacaagcctc taacaactgt
    17941 tcataatgct tgattaactg cgcataattc attatttatt taaggcttct aacttttgag
    18001 tctgatcata cataacaagt gcatcaataa cttcatccat ttttccagat aaaatagtat
    18061 ctaatttttg aagtgtcaaa ccaatacgat gatctgttac acgattttgt gggaaattat
    18121 aagtgcgaat acgttctgaa cgatcaccag taccaactgt tgatttacgt tcagcatctt
    18181 gttcatcttg agcaatttgc gcaaaatggt cagcaacacg agcacgaatg attttcatcg
    18241 ctttatcacg gtttttttgc tgagtacgct cttcttgcat ttcaactttg attcctgtag
    18301 gaatgtgtac catacgaact gcagtagcaa ctttattgac gttctgacca ccagcaccag
    18361 acgcgtggta aatatcgacg cgaagatctt tttgatcaat ttcgtattct acttcttcaa
    18421 cttctggcat taccagaaca gttgcagttg aagtatgaac acgaccctga ctctctgtca
    18481 ccggaacacg ttgaacacga tgagctcctg attcatattt aagcttagaa taaactgact
    18541 gaccagaaac catagcaacc acttctttaa taccaccaac accattaacg gatgattcca
    18601 taacttcaaa acgccatcct tgagtttcag catatttttg atacattgtt aaaagatcac
    18661 cagcaaaaag cgctgcttca tcgccaccag cagcaccacg aatttctaaa ataatgtttt
    18721 tatcatcgtt aggatcttta ggtaatagaa ggatttttaa tctttcttca tactcttctt
    18781 tagctgcttt tgattcttta agttcttctt tagccatttc ttccaattca gcatcaccag
    18841 aagcatcctt gatcatttct tcagcatctg aaatattttg aatgacttgc ttatattctc
    18901 tataagctgt gacagtttct cgagtattag cttcttcacg tgataattcc ataaatcgct
    18961 ttgtatctga aacaacatct ggatcactaa gtaactcacc taactcttca taacgatctt
    19021 caactgcctg caattgatca taaatattca tttttctttg tatctccttt aaaaccgatt
    19081 atcttttaaa tatctggatt aaaataatgt tttcgacaaa ctgggatata tgtttcatta
    19141 ccaccaattt gtatctgatc accttcatag acaggttttc cgttttctgt ccttaaaacc
    19201 atggttgctt tcttactaca gtattggcaa attgttttga tttcatcaat cttatcggcc
    19261 aataatagca aatgttttga accttcaaaa agattgtttt ggaaatcatt tttcaaacca
    19321 aaagccatga ctggtacatc taagtcatct actactctag ctaaatcgta gacgtttttc
    19381 ttactcaaaa attgacattc atcaattaaa acacaataag gcttttgtgg taaattctga
    19441 atataggaga aaatgtccat atcatctgaa attggaactg cttcacgacg cattccaata
    19501 cgacttgaaa cgactccaaa ttcatctcga gtgtccaagg cgcttgtcat aataaccaca
    19561 ggttttcctt gttcttcata gttatgagca acttttaaaa tttcaatggt tttaccagaa
    19621 ttcattgtcc catatttata atagagttga gccaaagcgg aaaatcctac tttctatagt
    19681 attctaccta ttttaccaca aaggctgaag ttttcccagc cattgaaaac agtgtgtact
    19741 ttttcacaac attatgttac aatgatgccc aataacataa aggagtaata ttatgccatt
    19801 tgtaaaaatt gacttgtttg agggacgctc acaagaacaa aaaaatgaat tagctcgcga
    19861 ggttactgaa gtagtatcac gcattgcaaa agcacctaaa gaaaatatcc atgtttttat
    19921 caacgatatg cctgaaggaa cctactatcc tcaaggtgaa cttaagaaaa aataagaaat
    19981 agtaaaagag gttacccagg taacctcttt tgatagtctt tgcaagcata tttcttgaca
    20041 aattgaaaat atttgaaaat aagtattgat tttcaagtta gaaaacggta tcattagaat
    20101 atattgcttt gggggtggaa aatttgcaac tttctcatag tttaagactc atgggaacga
    20161 ctatcgatat tcaaattaat tctaaaaatg ctcaaaaaca aatccaagag gtcatagaac
    20221 tcttggagct ctacaaaaat cgctttagtg caaatgactt taattcagag ttaatggcta
    20281 taaataacaa cgctggtatc aagcctattc aagtacatcc agacctcttt gaattaatca
    20341 caattgggaa agaacacagc ttagctaggc ctagtaacct aaatattgct attggtcctt
    20401 tagtgcaaac ttggagaatt ggattttcag atgctaaact acctagccct agtgaagtat
    20461 cagaagctat gattttatct gacccaactc atatacttct agattcaaaa aatcaatctg
    20521 tttttttaaa tcagatagga atgaaaattg atttgggtgc acttgcaaaa ggatatattg
    20581 ctgacaaaat tatgacctat ctcaaaaatg agatgattga ttctgctatc attaatttag
    20641 gcggaaacgt tttagttcac ggagataatc ctaatcgttc agaagggtat tgggttatcg
    20701 gcattcaaca tccgaaaaag aaacggggac aaaatattgg agctgtcaaa attaaaaacc
    20761 aatctgttgt aacctcaggt acttatgaac gccgtctgat tattgacgat aaggaatacc
    20821 atcatatttt tgatcgacag actggttatc cgatacaaac agagatggct agcatttcta
    20881 ttgtttctaa acaatccgtc gactgtgaaa tctggaccac aaggttgttt ggcttatcca
    20941 ttaaagaggc tcttgatatt ttaaacgctg tttcctacat tgaaggcatt atcatcacta
    21001 aagatgaccg tatctattta tctgatggct taaaacatca ttttcaacta ttttatcatt
    21061 aagaaagagg atttctaata tgaaacttat tggcatcgtt ggaactaact caaataaatc
    21121 aacaaatcgt caattattac aatacatgca acaacacttt gctgacaaag cagaaattga
    21181 actcatcgaa gttaaagacc ttccgttatt taataaacct gctgataaaa atgttcccca
    21241 agtaatttta gatattgctg ctaaaattga agaagctgat ggtgttatta ttggtacgcc
    21301 agaatatgat cattctatac cttcggcctt aatgagtgtt ttagcttggc tatcatatgg
    21361 tatttaccca cttctcaaca aacccgttat gattactggt gcttcatatg gaacacttgg
    21421 atcatcacgt gcacaattac aattacgtca aatacttaac gctcctgaac taaaagcaag
    21481 tgttttacct gacgaattct tgttatcaca ctctctccaa gcctttgata aagatggcaa
    21541 cctacacgat atcgaaacaa gtcagaagct agatgctatc tttgatgatt ttcgtctttt
    21601 tgttaagatt actggtaaat tatcaaatgc tcgtgattta cttcaaaaag aagcagaaaa
    21661 ttttgactgg gaaagtttat aagagtatag aagggaataa ctaagatgaa atttgtaggt
    21721 attgttggct caaatgcaga gcaatcatat aatcgtatgt tgcttgaatt tattcgcaag
    21781 aattttaaaa ccaaatttga attggaagtc cttgaaatcg atgatattcc aatgtttaat
    21841 caagtccaaa attgggaaga aagtttccaa cttcgtcttc ttaataataa gatcacacga
    21901 gctgatggcg ttattattgc cactcctgaa cataatcata ctattacggc cgctcttaaa
    21961 agtgtgcttg agtggctctc atttgctgtg catccactcg aaaataaacc tgttatgatt
    22021 gtcggtgctt catattatga tcaaggcaca tcacgtgctc aaatacacct tcgtaaaatc
    22081 cttgatgctc ctggcgttaa tgcttacaca cttccaggaa atgaattctt attaggaaaa
    22141 gctaaagaag cctttgatga taatggaaac attatcaatc ctgggactgt tcaattttta
    22201 gaaacttgtc ttgataattt tatcaaatat gtaggagttg tatcatcatt gagaaaacct
    22261 aaagctattg aatctgaaga cctctactgt cagggacaaa tcgatacaac cattactagt
    22321 atcgatccag atgatcctga ttggttagac aaggcagccg agaaagttaa cgccgttgaa
    22381 ggggatgctt acgttaaact agacaatggt ttacttactg ttaaccaact taatatgttt
    22441 ttaaatgcta tgccatttga gctaacatat gcagatgata ataatcaatt tctatattac
    22501 aataataatc atccagatcc agatacaatg ttaggaaaac gtgttcgtga acaagtagga
    22561 aaccgtatgg ccacagttca tggtagttta ccaccggcac gaatgaaaaa tgttgaatgg
    22621 gttatcggta ctcttcgtaa tggaaatcaa gaatatgtac gtacagtcgt tcctggtaca
    22681 cctccagaag ttatcaatac tcacaactat caagctatgt actacccaga cggatcttat
    22741 gctggtatta atgaaattat ctttaacttt aaaccgtggt tagattggta cctccaaact
    22801 actggacaac gtttggtcgg tggaactact gctgctcctg gagcttcagc agttgacgct
    22861 acttctagcg cttcaactag cacagaggat gctcccgttg atcaagccac agatgccact
    22921 tctggtgcct ctgaacatta atctcctcgc ttttgaggat ctgatacctt aaaaagatat
    22981 caagatcctc ctttttaata tttaatcaag ttaataaaat cacataaaag gaatatacta
    23041 tgtcactttt tcaagaaaaa attgcttaca attgcgctaa aaaggaagcg ctttataaag
    23101 agagtttagg acgctacgcc ttgagatcaa tgctagcagg ggcttatttg acaatgagta
    23161 ctgctgccgg tatcgtcgca gctgatacta ttggtaaaat ttctcctgct ctatcaggtt
    23221 ttgtatttgc tttcatcttt agttttggac ttatttatgt tttaatattt aatggtgaat
    23281 tggcgacatc taatatgctt tatctcactg caggagccta taataaaaat atctcttgga
    23341 aaaaagccat aacaatttta atttattgta cttttttcaa cctcgttggt gcttgtatat
    23401 tagcttggtt gtttaatcaa tcgtactcat tccagcattt aacaaatgat agctttttag
    23461 ggcatgtcgt tgctaaaaag ttaggaaaac ctagttcagg agctttccta gaaggtatta
    23521 ttgctaatat gtttgtgaat ttagctatcc ttgcttacat gttattaaaa gaagaatcgg
    23581 caaaaatgac ggttatttta tctgctattt ttatgtttgt ttttctatca aacgaacact
    23641 taattgctaa ttttgcatcc tttatgttag ctgcttttag ccatatcgaa catattaaag
    23701 gttttactct tctaaacgtt atccgccaat ggacacttgt attttttggt aactggatag
    23761 gagggggcgt tttcatcggc ctagcctatg cttggttaaa taaaacaaaa acttctcacc
    23821 atgaataaaa gaaaaactga gtaatttatt actcagtttt tcttttatat tctcatcatg
    23881 aaatcaataa ctaaaaatta acttttttga taaataaagg aaagtatcat attaattttt
    23941 taggcaatcc attcaaaatg atatttaaaa caacagctgt cagggttgag ataacaatac
    24001 catttgtcaa aaacatttgt aaagtattag gaagactaat gaagagattt gtctcattaa
    24061 aacctactcc tgctgcaatt gatacggctg caatgataaa gttatgctca ttatgctcaa
    24121 aatcaacctg attaagcatt ttcatccctt gaagtgctac cataccaaat aaaactaaca
    24181 tagcaccacc aagaactgga cttggaatca tttgagccat tgcgccaaat ttaggtaaca
    24241 agccaagtat aactaagaag agagctgtaa aatatatcgg cttacgcgta cgtatcccag
    24301 atatctgtac cagtcccaca ttttgagaaa aaccagtata tggaaaggta ttaaataagc
    24361 cgctaagtaa tactgccaat ccttctgaac ggtaaccatt acgaagtctc ttactgtcta
    24421 atttatcgtt tgtaatatct gaaagcgcaa ggtaaacacc tgttgattct accatagaaa
    24481 ctgttgcaat aatacacatc attaaaatag aagtaaattc aaatctagga gctccaaaat
    24541 aaaatggctt cggaatatgt acaagtggtg cttctgctac cacagaagca tcaactaagc
    24601 ccataaatgc tgcaagaatt gtacctgaaa tgagtccaat aaggattgaa attgacttca
    24661 aaaagccttt tgcgaaaata ttaattaata aaacaacacc aatcgttacc agtgataaag
    24721 ttaaagattg taagcttggt tcttttgcat tatcacccat atttcccatc gcaactggta
    24781 ttaatgtcaa accaattgta gtaataactg atcctgttac aattggagga aagaaatttg
    24841 caactttaga aaaaatgcca gcaactaaaa cgacatatat tcctgaagca attaaagctc
    24901 caaacatata gccagaacct tgttgtgcac caataattga taaaggagct acagactgaa
    24961 aggcacaccc taatacaact ggaagaccca ctccaaagtg cttacttaat cgtaattgta
    25021 ataaggtggc aatcccacac ataaaaatat ctgtcgcaat aagataagtt agttgcttag
    25081 cattataacc aagagcacta gcaatcatga taggtacaag aatggatcct gcatacatag
    25141 ctaacaggtg ttgcaagcct agaagtgctg cttgcgaatt actttgctca ttaaatttca
    25201 ttaagcatca gcctccgcaa aaacaactcg accattttca aacgctttaa tacgagcaag
    25261 agaagtaacc ggaacacctg ttttttctaa caaatcacgc ccatcttgga aagatttttc
    25321 aataacgatt ccgataccag caaccttagc ttcagcttga ccaataattt caagtaatcc
    25381 tttagccgct tgaccgtttg ctaaaaagtc atcaatgatg agtacagtat catcgttaga
    25441 taaaaagcga ctcacaatag aaacttgact cgtaacttgc tttgtaaaag aatacacttc
    25501 agcagttaag ataccttcag tcatagtaat gttcttagct tttttagcaa atatcattgg
    25561 tacgcccaat gcttgagctg cgtacactgc tggcgcaatt ccagatgctt caattgtaac
    25621 aaccttcgta atgccggctt ctttatattt atcagcaaaa actttaccta tttcctgcat
    25681 taactcaaaa tttacctgat gagtcaaaaa agaatcaact tttaaaatgt tctcacctaa
    25741 aacatcacca tctttaagaa tacgttcttc caataacttc ataataaccc cttcactctt
    25801 tagaaaataa aaaaataact tacctcaatc agaggacaca aatctcctct aatttatcgc
    25861 ttcattaaca tgaaccaaca ggcaagctat ttattagctt acttattgtt agacattacg
    25921 gtgtcttgta gagacgctaa gccaatcctc agcataaata agtaaatcat atatttaatt
    25981 gttatgtatg atagataact attgtaacat ttttaattaa gatttcaaga tttaatttaa
    26041 atggaatcac cattccaaat agaattttta acaataacgt aatcaacgtg tctcaagcta
    26101 tccaattctt ttccacctgc gtaagaaata gaactttgca aatcttgttg catttctgtc
    26161 aaagtatcct ctagtcgccc tttaacaggt aatagaattt tcttaccttc aacattttta
    26221 tgttctccct tttgatattc agaagctgag ccatagtatt ctttaaattg ctgtccttct
    26281 acttccacaa gttttccagg actttcaagg tgaccagcaa acagcgaacc aatcattacc
    26341 atactagcac caaagcggat agattttgca atatccccat gtgttcgaat accaccatca
    26401 gcaataattg gcttgcgagc agctttagaa caccaacgta aggcagctaa ttgccaacct
    26461 ccagtaccaa aacctgtctt gactttagta atacaaactt taccaggtcc aataccaact
    26521 ttagttgcat ctgcacctgc attttccaat tctctaacag cttcaggcgt tcctacattt
    26581 ccagcaataa cgaaagttcc tggaagctct tgtttaatgt gttggatcat ctctataaca
    26641 ctgttagaat ggccatgtgc aatatcaata gtgataaact caggagcatc ttctttcaga
    26701 cttgtcacaa aatcatattc atagtctttg accccaaccg aaatcgaagc aattaagcct
    26761 ttatcatgca tacgcttgat aaaaggtctt cttgcttctt cattaaaacg gtgcataata
    26821 taaaaataac cttcacacgc taatgtttca gctacctctt catcaataat agtctgcata
    26881 ttagcaggga ttactggaag tttaaaggta tagtttccaa gtttgacact tgtgtcagct
    26941 tgtgagcggc tagatataat acacttgttt ggtattaatt gaatatcttc ataatcaaaa
    27001 acaggaatat cgttaaacat aatggttctc ctaatgagta aaatgacctt cctataactt
    27061 tgtataacaa agcatcgcta ggtaaaaaca tacctttcct ttttaaatta tatactttat
    27121 ttatttttag tcaatttttg atcaatttat ttgaataatg ttcgtttttt tcaaaataaa
    27181 tgtaaaatcc tatagagcag tactctatag gattttacat ttattatatt gcttttcttt
    27241 ttcgaagata agttaatcct acaatagcta agataacaac aacgataacg aatagaactg
    27301 caaattgact accaattata gctccatttt tcggaatagc tacttgtccg atggtaaaaa
    27361 tttgtgaggc tactgcggtt gcaaaagctc ccgcaaattg ttgtagtgta tttaagatac
    27421 tattaccatc cgctgactgt gctggcttta gttttgttaa agctgtcgta accatattac
    27481 ttgctccaat ccccatacca attttagtaa gaatatcaaa ggctaaaagt actacaacaa
    27541 acttcgcagg aatgaatata gctgtaccaa ttaaacctat caaagaaagg gagataccta
    27601 tcataattgg cttaaatgca ccaattttat ccaggagata acctgcaaaa ggggataaaa
    27661 aaactactgc tattgcacct ggaaacataa acaaaccagc tttggttgaa gtttgatgta
    27721 aaacaagttg caagaggtta ggtaataaga acgaaagtgc tagaggaatc atttgaaaag
    27781 ccaggacgcc atacaataag attgagaaat ctttatcttt aaaaacattc aaattcaaaa
    27841 attctagctg tttatttaaa tagtaaaaaa taacaaaact aacaatagct actaataagt
    27901 aaaaaataga catcttttca atagctaaaa gaagggttgc caagccaata cctaaactga
    27961 caaaggcagg aaaattgagt ttatcattaa ttcttacttg acgtttggga atagaagcca
    28021 aacctataaa ggtagatatg ataagtattg gtgccagaag cataaaaatc atcttccatc
    28081 ctaacattcc tgaaatgaca ccaccataag taggtccaac tgccggtgct aaaagtgtga
    28141 ccatagcagc tactcccatc attagtcctc tacgctccat cggaatttgt gtaagaatga
    28201 tatgaaacaa taaagggagg gctaatcctg tcccaacacc ttgcaagact ctccctacta
    28261 acaatatagc taaattaggg gcaaagctat caattaaaac accaaataaa aaaagcacat
    28321 ttgccaatgt aaaaatttgt ctctctgata aatttttaat cataaatgct gacaaaggca
    28381 cagtcacagc aacagctaac aaatttcctg ttgtcaccca ttgaataaca gcggggttaa
    28441 ccccaaattc tttcatcaaa agcggaaaag tgacattcat agatgtttca ataagaatcc
    28501 ctgaaaaacc taatatagcc gttgtaatta tggcaggcat aatacgccat ccctttttag
    28561 tcattaaaat ctccttactt tgattattat cataaaatag agcaccccgc taaatcaagg
    28621 tgctccattt aattcattcg aattaaaatt aaattgttta tattagttgt caaaggacaa
    28681 tcaacagctc ttattatatc aaataaagct aagaaaaaca aagagttttt tgaatattaa
    28741 tgccttcact ttcttatcta ttctctaccc atcaatcacc acttcagaga acataacaat
    28801 tgcaatttac tcttaatacc gttatcatgt tcatatgcta atacccaaga aaatagtggc
    28861 ttttgcctca aatgaatctg aaaataattt ctttattacg ttaaagactt actttaatta
    28921 tttgttttct attcaaatca ttacagatat ttcaactctc aatcatgcag attttgacgg
    28981 tagctttgca ttccatgata tagaaactag catacctcat ctggtaattg actccaacta
    29041 cttagcgatt agtcagacta actcaaaaac agaggctaac gatattaaaa cgttctcgga
    29101 actctccaaa acaatgaccg agttttacta tatgctaaat tttgacttat ttaaccatct
    29161 cccatatcgt tttagattgc ataataagga cggtcaaaca atttattcga accataaacc
    29221 tgaagatcct tttgatattt atcctgaaga agaatatcct atagacaaat gggttcaaaa
    29281 cagtctaata gagaaaaaag cgaaagagct acatttactc ttacctggcg catcacaaga
    29341 ttatattcta gtacaatctt ataaacgctt agaaaatgat agtgggcaac tagttggtta
    29401 tattgaacat gtccataata ttaaaccact attagagggg tatttaaaag aatccggtca
    29461 agctatcgtt ggatggtctg atgtcacgtc tggtgcttct atctctaacg atgattttga
    29521 actataatcc actcgtcact ctaaaaagaa aactaataac taaaaggcac tggtttaatt
    29581 ccagcgcctt ttattaatca atctccagtt gttgtaagtt caattttttc tttaattggt
    29641 agacctcgat tagctgtaaa aatcaaagga actacttctg ttgtaacttc ggaaaattgt
    29701 agaccaaacc atcgtattgg tgttgctgtc caatgcttta cagatgattt cattagcata
    29761 acaaatcgtt caccaggttt taggttttga ctgcttgaaa aacgttcatc aaggacaata
    29821 aacctaaaat ctcctacttt actatcaggt ctaacgctgt atgtctttcc ttgtttaggc
    29881 agacgtccac tttcaagtaa ttcttctaca atcgtacgaa gatagcgtga cacagtctga
    29941 cgcatcttaa atccaaggta aagttctacc ttcacaatga agtcggttcc catcatgtca
    30001 actttatact cagctgtata aggttcatca gtgaccttaa tgtttacaaa ccaataaact
    30061 tgtgcttttt tagggcgctt atcaaggata gaatacatga tagacttatc aatcatgtct
    30121 ccttccatac gatttgtcaa ataaacaacg ttagtatgat aaatcggata acgatgatca
    30181 tgtctaagtt tatctaattg tccgatgtaa tcttttaaat ccaacaactt aacataacgc
    30241 gaaacaattt tactaccttt ataccagata gtcatgatga atataattgc tactgctata
    30301 ataacaacaa catagcctcc atgcataaat ttaacagctg aagcaaggaa gaagattccc
    30361 tctaaaatac caaagaaaat catcatcaat aagactaaac ctctttttac tcctttttga
    30421 attaaaaaga aagacagtaa aatagttgtc attagcatcg taattgttat cgctaatcca
    30481 tatgctgctt ccatgtgcgc tgaagtctta aaaagcaaaa caatagaggt tgtaatggca
    30541 aataagaacc aattaataac aggtatgtag gtttgaccaa tattgtcacc aggataagtt
    30601 gaacgaaatt gtgggaatat ttttagtcgc atagcttcag aaactaaggt aaatgatcca
    30661 gaaatcagtg cctgtgaagc gatgatagct gccaaagtag ctaaaataac gacatgcatt
    30721 gtaaattgcg aaggaatact agcaaaaaag ggattcaatt catttcctgc gttcttatta
    30781 gctaaaatcc atgccccttg cccacaataa gaaagtataa tggcaacctt aacgaacggc
    30841 catgaaacat gtatatttcc acgcccaaca tgacctaagt cagagtatag tgcttctgct
    30901 cccgttgtcg ccaggaaaat agatcctaaa ataaaaatac ctttatgatt ctctggacta
    30961 aataacaatt tcaaaccgta gtatggatta attgctttga aaacttctgg atgggcaaaa
    31021 ctattaagga gaccgctaat acctaaaaag gcaaaccata taaacataat tggcccaaat
    31081 aatttcccaa taacacccgt tccaaacctt tggatggcaa aaagtaacag taaaataaac
    31141 aaggtcgcaa aaataacatt actctgattc tggaaaatat gctgcaaact aggaactact
    31201 tttaatcctt caacggctga agttacggtt acagctggcg tcaaagctcc atctgacaac
    31261 aaggttgcac ctccaataac agccggaaca attaaccaag gtgtcatttt tctaacaagg
    31321 gtatataaag aaaaaatacc accttcgtgg tgattatccg cctttaaagc tacaagcaca
    31381 tacttgatag ttgtaataag tgtcaaggtc catatgatta aagatataga acctaagata
    31441 aacgattctg tgacactaga aataccacct tggttttcaa ccaacgattg catcgtatag
    31501 agtgggcttg taccaatatc tccataaaca atgcctaaag caataataaa tcctgctttt
    31561 gatgctttat caaaagaaga atgattgaca tgttgcattt ctgccaaccc tccgattaaa
    31621 taatattagt caaaaaaatg gctgaactca ttatagcaga ttaagttaaa aaaacaacac
    31681 ttatctataa aaagaccaga aattattaaa gcgtttcctg gtctttacta ttttaatcac
    31741 gataaacagg ttgaggtcca taagtttgac tgactggcat tatttctatg cgattaatat
    31801 taatgtgttc tggttggcta gcaacccatg acacggtatt agcaatatcc tctgcttgaa
    31861 ttgcatgcgc accttcataa agtttttcaa ctcttttatg atctcctttg aaacgtacag
    31921 ttgaaaattc tgtcccttca cataaaccgg gttctaggtt agtaactctg attttagttc
    31981 cagctaaatc ggcacgcaag ttgagagcaa attgttttac aaaagctttt gatgcgccat
    32041 agacattagc tcctggataa ggaattgttc ctgcagttga tcccaaattg ataattaacc
    32101 ctctattaac ttctaccatt ttcggtaaaa tgcatcgtgt caagtaaatt aatcctacaa
    32161 cgttagtatt aatcatagtc atccaatctt caaaatcagc ttcatatgat ttatccaatc
    32221 caagtgctaa acctgcatta ttaaccaaca aatcaattga ttgcaaattt ttaggcagac
    32281 tttctaaagc tttgtcaacc gattctctac ttgtaatatc catttaaagt ggataaaagt
    32341 aaccctctga aaacttttct ccaaggcatt ttaatttatc cattcgacga gcacaaccaa
    32401 taacgctata accatctgaa actaatttag taacaacagc tgcaccaaaa ccagcagatg
    32461 cccccgtcac aagcgcaact ttacgtgata tcataaatac tcctcctcta gtatatagaa
    32521 aaacgagcta ggaaaaatcc ttctcgtttt ataacttatt ttgatttctc atcaatagct
    32581 tgagcagctg taatgatagc aagtttgtaa atatcttcag cacttgatcc acgtgataaa
    32641 tcatttactg gagcattcaa tccttgtaag atcggtccga ttgcttcaaa catacccaaa
    32701 cgttgtgcaa ttttatagcc aatatttcct gattgaaggt ctgggaaaat aaaaacattt
    32761 gcttttccag caacatcact attaggagct ttaatttctg cagtttccgg aacaaaagct
    32821 gcatcaaatt gtaattctcc atcaacagct aattcgggag ataaatcttt ggcaatttta
    32881 gctgcttctt gaactttttc agcttgaggc gcttttgcac ttccttttgt tgaaaaacta
    32941 agcatagcaa tcttgggatt aatatcaaat attttggctg tatccgcagt atttactgca
    33001 atttctgcta gctcttgtgc attaggatca atgttaatag cacagtcagc aaaaatataa
    33061 cgttcttgtg tattttcacg gttcatcaag aatacacctg aagtgcgtga aattcctggt
    33121 tttgttttaa taatttgaag agcaggacgt acagtatcag ctgttgaatg aatggcccct
    33181 gataccatac catctgccaa accaagtttt actaacataa ccccaaaata gttaacatcc
    33241 ttcaaaagac gatctgcatc ttccatagta gctttgcctt tacgaatctc aacaaaagct
    33301 tctttcatct cttcaaattt ttcgtattgt tcagggtcaa taacgctata atcttgatca
    33361 acaaaaccaa gtcttgccaa agaatcgtga acttcgtcag ccttacctaa aataacagga
    33421 tccacaagac cttcaaattt caaacgagct gctgcacgta atacacgttc atcgcttccc
    33481 tcagggaaaa caatcttaac ataatcactt gcaacttttt ctcttaaacc accaaataag
    33541 gaacgcatat atataaaacc ttctatttct aaattaatgt aattccatga taacgctgtc
    33601 aaagtcatct gtcaagtcta gcgtctgttt atttattttt cccataaaag ggtcgtaaaa
    33661 agacaaagaa tgacaatgca atgcttgcct gtttattcct agatccatac gtcctccgta
    33721 caaatcatct cccaaaagag gaaagccgat atgagcaaag tgaactctaa tttgatgagt
    33781 acgccctgta tgtaatttga tatccactaa tctaacatca ccataacgag caactacttg
    33841 ataactagta tgtgcatatt tgcctgattc atggactcgc ctcgtaataa tactatcaac
    33901 atctcgtgca ataggagcta taatatcacc actatctgct aaatcaccac tccctgatac
    33961 taatgcataa tacctttttt caattgcttt agcctgaagt tgtttatcta gacgagcatg
    34021 ggcataacca tgcttggcga ataacattaa tccacttgta tctcgatcca accgggtcac
    34081 aatatggacc tgctgattgg catagttatt agacacataa tagtgtttta taaaattagc
    34141 aattgtattt gaatgaatag agctaggaat actagcgaaa ccaaaaggtt tatttaaaat
    34201 caaaaaatga tcatcctcat aaacaatatc taaatcgtga ggtactggtt ccaaagtttc
    34261 gtgagaaggc tcgtcaggaa tatctatcgt aacaacatcg ccaatctcta ataagaaaat
    34321 agcgttctgc tcttcaccat tgacaaaaat cttaccacct ctgtacttaa tctttgctaa
    34381 taaccccctt gaaacatcat gacttttcaa aagtgtttta actttgcacc tttcttctgc
    34441 tatgtattca aatttcaatg ttcaggttct ccgataaaag catcgttaac acgttcccaa
    34501 aagctagtgt gacttggagt tgaaacaaag ttaatacttt tttcgtcaat actatattct
    34561 attttggtaa catttttgta atggaccgtt ttgttatcta tagaaattgt atagacacca
    34621 actcgtttag gaactatctc tatagcatct tttttgggga tgataaccga tgaccccaag
    34681 gtacgataga cgcgattatt aagactagaa atttctgtta gttgcaaggc ttctatcgtt
    34741 gggtggagta ctgccccccc tagtgattta ttgtaagcag tactaccggt tggtgtagaa
    34801 actaaaatgc catctcccct aaacctttca aataccactt gattaataac aacatccgct
    34861 accattgttt tttcaatacg cttaatagta gactcattta aagcacgcgc acgaataact
    34921 cgaccatctt caagggtaat agttactttc aaaataggat aggagatttg ttcaccttta
    34981 tcatttttca aattattaat taatgtgtca acttcaaaat ctcgatagtc tgtataaaac
    35041 cctaaatgtc ctgtatgaac accgacaaac cttactttat ccagttgttt ttcgtacata
    35101 tggaaggctg acaaaagcat accatctcca ccaatagaaa tgacaatatc aggatccttt
    35161 tttgataaat aaaaatcagg atcatgttta aaagcagcaa agagctttga ggctacacgc
    35221 ttgctttgat attttccgtt agcaattata gcaacacgtg ttgctctatc tgtaaaattc
    35281 atctgtgtca tcactatttc ctacaccatc gctcaatttt cgatttaaag gatcaaataa
    35341 aagctgtgct tcacgaatat cttcgcgaat cttacgcatt tcttcatcca attctaaagc
    35401 gatttttgct gttttctcca gtctttgttt aatttcttca gggaagtccc cctgatactt
    35461 gtaatttaat gaatgttcga tggttgccca aaaattcata gccagagttc taatttgtat
    35521 ttctgctaaa actttctttt gtccatctat tgtgtctaca ggatactcta caacgacatg
    35581 atatgaacga tatccactag atttcatatg ggtaatatag tctcgctcct gaaccactgt
    35641 catgtcatga cgttttctca acaaggcaag gacttcatca acgtcatcta caaattgtac
    35701 catgatacga agacctgcaa tatcttgcaa gtcctgagca agattttctt ctgaaatacc
    35761 acgtaaaacc atcttttctt gaatactttc aaccgacttt acgcgtccag taacaaattc
    35821 tattggagag tgacgatttt gctttctaaa ttgtttacga ataccacgaa gctttatttt
    35881 taattcacca accgtttgaa tataagggtc tagaaatgtt tcccaatcca ttgacattct
    35941 aatcctccaa aatatttaat gtactctctt gtaaaataaa ctttttttct ataaaattaa
    36001 cacaacaatt aatattatac catatttagg aaaaggaagt gataagatga cacacctaga
    36061 aattgaatac aaaactctat tgaataaaga cgaatttaat cgtctaacaa gcttattttc
    36121 tcatgttcaa cctattactc aaaccaatta ctattttgac acagaaacat ttgaaatgaa
    36181 agcacaccga atgtcacttc gcattagaac gttacctaac cgtgccgaac taacactaaa
    36241 aatccctaga gaagtaggaa atttagagca caatcatgac cttactttag aagaagctaa
    36301 atatatcgtt aaaaatggcc agttccctga agatactgag attgcttctt taatccttga
    36361 aaagggagtt gatccaacca aattagctgt ctttggacaa cttacaacaa caagacgaga
    36421 gatggaaact tcaatcggct taatggctct agacagcaat atatatgctg atattaaaga
    36481 ttatgaactt gagttagaag tcaaacaacc taaacaaggg aaaagagatt tcgaccagtt
    36541 tttaaaagaa aataatatta attttaaata cgccaaaagt aaggtggcta gattctctgc
    36601 tactttaaaa aataactaac gttgagagat tggattttcc aatctctttt ataattctca
    36661 tttaagcaag aacttaaaat ctcatactat ctctcctcaa cattgttatt gcaagcattt
    36721 tcatgtttct tatcgtgaat tttttctatt tttttggtaa actattagta tcaatctaaa
    36781 ggagtcatct atctcatggc agaacaatac gccgataaac aaatcaaact tttctcatta
    36841 accgctaata gagaaattgc tgaaaaaatt tcccaagctt caggaattcc tttgggtaag
    36901 atgtcatcac gtcaattctc tgatggcgaa attatgatta atattgagga aacggtgcgc
    36961 ggtgacgata tctatatcat ccaatctaca agcttcccag tcaatgataa tctctgggaa
    37021 ttactcatta tgattgacgc ttgcaaacgt gcaagtgcta atactgttaa catcgtagtc
    37081 ccttattttg gatactctcg tcaggaccgc atcgctgcat ctcgtgaacc tattactgca
    37141 aaattggtcg ctaatatgtt agttaaagct ggagttgatc gagttcttac cctcgactta
    37201 catgcagtgc aagtacaagg gttcttcgat atcccagttg acaatctttt tactgtccca
    37261 ctatttgctg aacactataa tcaactcggt ctctctggtg aagatgtcgt cgttgttagt
    37321 cctaaaaatt ctggaatcaa gcgtgctcgt agtttggcag aataccttga ctctccaatt
    37381 gctatcattg attatgcaca agatgattct gaacgtgaag aaggctatat tattggtgag
    37441 gtagaaggta agaaagctat tattattgat gatattttaa atacaggaaa aacctttgcc
    37501 gaagctgcaa aaattttaga acgtggtgga gcaactgaga tttatgctgt tgctagtcat
    37561 gggctttttg ctggcggcgc agctgatatt ttagagtctg caccaattag agaaattatc
    37621 gtaacagact ctgttttatc taaagagcgt attcctagca atattaaata tctgaccgct
    37681 agccatctca ttgcagacgc tattatccgt atccatgaaa gaaaaccttt aagtccatta
    37741 ttttcatatc gttcagataa gaaggattaa ttgatgattt atttagacaa tgctgctact
    37801 accgctctaa ccccatctgt tattgagaaa atgaccaatg tcatgacaag taactatggt
    37861 aatccatcta gtatacatac ctttgggcgt caagcaaatc aacttttacg tgaatgtcga
    37921 caaattattg ctgaatatct aaatgttaat tcacgtgaaa ttattttcac ttctggggga
    37981 actgagagca acaatacagc tatcaaaggg tatgctcttg caaatcagct aaaaggtaaa
    38041 catattatta cctctgaaat tgaacatcat tcagtcctac atactatgac ttacttatca
    38101 gagcgatttg gttttgatat tacttactta aaaccaaacc atggacaaat tactgcaaaa
    38161 gacgttcaag aagctttacg agatgatact attatggtat ctctcatgtt tgctaataat
    38221 gaaaccgggg actttttacc aattcaagag attggtcagc ttctcaggaa ccaccaagct
    38281 gtttttcacg ttgatgccgt tcaagtcttt agcaaaatgg aacttgatcc tcattcttta
    38341 ggaattgact ttttagctgc ttctgcccat aaatttcacg gtccaaaagg tgttgggata
    38401 ctttactgtg ctccccatca ctttgatagt ctacttcatg gtggagacca agaggaaaaa
    38461 aggcgtgctt caactgaaaa tataattggt attgctggaa tgtctcaagc tcttactgat
    38521 gctacgacta acacccttaa aaattggact cacattagtc agctgagaac gaccttttta
    38581 gatgctattt cagaccttga cttctatctt aataacggtc aagactgctt acctcatgta
    38641 cttaatatag gttttcctag acagaataat ggcttgttat tgacacagtt agatttagct
    38701 ggattcgcag tttcaacagg ttctgcatgt actgcaggaa cagtcgaacc tagtcatgtc
    38761 ttaacaagct tgtatggagc caactcacca cgtctaaatg aatcaatacg tattagtttt
    38821 tcagaactaa atacccaaga agaaattctt gaattagcta aaaccttaag aaaaattata
    38881 ggagattaat atggcatttg aaaaagaaat aaatcttcca gactgcaatt acatttacac
    38941 tatcagcccg aacattaaaa aatatacctt acgcgatact acattcactc aaaatcgctt
    39001 aggtaattat gaattagagc gtctcctaga aacagtacct aattctggcg atggctttcc
    39061 tctaaagatt acaatcaata aagaactttc tggttttaag ctcttaatta ctgataaatc
    39121 tggccttaga atagtaaata tctttaaatc taccgatcat aaaattcttc aagacaaatt
    39181 ttatttctta atggacagcc tcgttgagcg taatatcttt acgaaaactg caaaataatt
    39241 taccgaccac tatttttaac attttagtgg tctttttgcg aaccattttg ttgtcacttt
    39301 cacaatcttt ttgtacaata gagatgtgaa gaattaagtt atataactga ttctaaatcg
    39361 aataataaaa tttttaacaa ggagataatt ttgtgattat ggataagtct attcctaaag
    39421 caactgctaa acgtttatca ctgtactacc gtatttttaa acgttttaat actgatggca
    39481 tcgaaaaagc tagttccaaa caaattgcag atgccctagg tatcgattct gctactgttc
    39541 gacgtgattt ttcttatttt ggtgaactag gacgccgtgg ttttggttat gatgtcaaaa
    39601 aacttatgaa cttctttgca gaaatattga acgatcattc tacaacaaat gttatgctgg
    39661 tggggtgtgg aaatatcggt agagctctct tgcattatcg tttccacgat cgcaataaaa
    39721 tgcaaatttc aatggctttt gatttagata gcaatgattt agttggtaaa acaaccgagg
    39781 atggaattcc tgtctacggt atttcgacta tcaatgacca tttaatagat agtgatattg
    39841 aaactgctat cctaacagta cctagtacag aagcccaaga agttgctgac atcttagtca
    39901 aagcaggtat aaaaggcatc ttgagttttt ctccagttca tttaacatta ccaaaagata
    39961 tcattgttca gtatgtagat ttaacaagcg aattacaaac tttactttat ttcatgaacc
    40021 agcagcgata aatagttcat tatttataaa atatctgctt cttctcgaaa actataatat
    40081 ttattttttc caacgatgat gtgatctagg cagacaattc ccagatgatc acatgatcgt
    40141 tttatttttt tagtgaaact taaatcactt tcactgggat ttggagaacc tgagggatga
    40201 ttatgtataa taatcaaaga agttgccatg tttttacaag cataatgtag aatttctctt
    40261 ggctctgcta ctgaacgacg tacagtacca ataaaaatag ttctctgttc gataatacga
    40321 ttttgtgtat ccatataaat agctactaaa tgttcttgtt ttttatcccc taattcgagc
    40381 atcattttcc tcgctaattg ttcactactt aaaatttgct ctttacgatc atattcagct
    40441 ttgtgaatcc gttttgctag ttctagcata gcttttattt cgacggattt aacctgaccg
    40501 attcctttaa tggattgcaa ctcctgtaag gataattgac caaaatctgc taaactgctt
    40561 atgttttcta aaatttgcgt tgaaatttca agaacaggtt tttctttaat acctgtacgt
    40621 aagagaatgg ctaataactc ctgattactc aatctatctg cgcctaaatc aactaggcgt
    40681 tctcttggta gtaaagcttc cttttttaat tcaatatggt acataaaaca cctccagtca
    40741 taatattcgt aaactaatca aaaactcatg ttttaaatca ataaaaaata cttagatgaa
    40801 aaatctaagt attttttatt taacgatagg ttcgtgcacg atttctaagc gtttgtcgct
    40861 tgataatatg atcttttaca atctgataga tagtagctgc aacaggaaca gcaagtaaca
    40921 tgcctaagat cccccataaa gcacctccga tagtaatagc cattaaaacc cacatagaag
    40981 gcagtccaat cgatccaccg acaactttcg gataaatgac atttccctca aattgttgta
    41041 aaaggatcaa gaaaagaaca aacaagaatg cttcagtaag cgattcagta gcaattaaga
    41101 tgaaaccgat tgtaacacca atgtaggctc ccacaaccgg tattagagct gtaaaagcaa
    41161 ctaaaacccc aactgttaaa gcataaggaa attggaagat caacatacca ataaccgtca
    41221 aacttcctaa aatcatagct tctaaagttt ggcttacaaa aaaaccatgg aaacgttgat
    41281 gaaggatatg acgaacgtaa tggaatgttt tgcctgttga acctaaatag gtatcaatta
    41341 acaaattaaa ttgacgtccc aactgctcct tgtttgccaa aacgtaaatt gaaaaaataa
    41401 aactaacaaa aacattcaga agtgttgccg caatagagga aactgaggtt agtaaatttg
    41461 ttaaaacaga taaaacttgc ttcaaaatct gctggctata accacttaaa gtagaaacta
    41521 agtctttatc tgttcccata taatttaaag cctcagaaat ttgtttattt tcattgagat
    41581 tattaaccaa tttagcaagt gctccggtat caataaccaa taaagaactc aaactagaaa
    41641 tcaaatctgg aatgacaatt gaaaatagcc agacaattaa accaataaaa gttgcatagg
    41701 ataaaatcat agaaacacta cgcttgattg ccattaatag tctagatcct ttaaaaagct
    41761 ttatatataa tcgctcatag actgacatta caatattaat aatataggca attccagctc
    41821 ccatcaaaaa aggaaatatt gccttatgaa gcgtagttaa aatattagaa acagctcccc
    41881 aataagcctg tatagcatag caaatagcaa atgttatgac aacataatag acctgccgtt
    41941 tttcaaattt catgaaatct ccttattaat cttaatttct ttcaattatt atacactatt
    42001 ttatgaatga gaaaaagaga gttaacagtt attagtctgc tctttattct atttgactgc
    42061 cattacttgc aataaccttc ttgtaccaat aaaacgattt tttcttgtaa cgcttgagtg
    42121 ttccctctcc tttattattg cgatcaacat aaatgaaacc atagcgtttt tccatttcac
    42181 cagttcctgc agatactaaa tcaatacatc cccatgtagt atagccaatt agattaacac
    42241 catctacata tatagcatct ctcattgcag caatgtgctg acggaggtaa tcaatacgat
    42301 aatcatcttc tacgtaacca ttctcatcag gaatatctac tgctccaagt ccattttcaa
    42361 caataaacat cggtttttga taacgatccc agatagcatt taaggtaatg cgtaatccaa
    42421 gcggatcaat ttgccatccc cattcagaag atttcaagta aggattctta agagatgcga
    42481 aaatattttc ttgaacctgt tcactgacag ttggattacc tgaagctact cggcttgaat
    42541 agtaagaaaa tgaaataaaa tcaaccgtat agtctcttaa caaagcaaga tcttctgctg
    42601 tcatttgaat tgaaatacct aaatgctcaa aatatttttt agcataatta gggtatttgc
    42661 cacgcgcttg cacatcaata aaaaaataat tttctctatt tttttgcatc gaagcccaat
    42721 aatcttgtgg atgacacgta tttggatagt attgacctgc agctaacata catccgattt
    42781 gactatttgg atcaacttca tgagcaattt ttactgcaat agccgaagcc actaactcat
    42841 ggtgagctgc ttgatattta atttgctctt gattttcacc atcttcaaaa tataagccag
    42901 cgcccataaa aggagcgtgt aaaatcatat tgatttcgtt aaaggtcaac caatatttaa
    42961 ctaacccctt aaaacgagta aataaagctc gtacaaggcg ctcataaaaa ccaatcattt
    43021 tatggctccg ccacccaccg taatgtttaa taaggtagat aggacagtca aaatgtgtga
    43081 tggttaccaa aggttcaata ccatacttac gacactcttt aaaaatattt tcataaaacc
    43141 gaagaccagc ttcatttgga tatagctcat caccctttgg aaaaatacgc gtccatgcta
    43201 ttgacatacg ataggttttg aaacccattt ctgcaagtaa tgcaaggtct tctttataat
    43261 ggtgataaaa atctattgat tcttttgctg gataaaagta tccttcctca aaatcaaaca
    43321 ttttcttttg tcctgaaata attgcaaatc gatcttctcc agtaggaaca acatctacat
    43381 ttgctaggcc acgtccatca acattgtaag ctccttcaca ttgattggca gcagttgccc
    43441 cgccccataa aaaacctttt ggaaatactt gttttaccat catttacccc tcttgttttt
    43501 tcttat
//
LOCUS       DATRUK010000012        42008 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.012, whole genome shotgun sequence.
ACCESSION   DATRUK010000012 DATRUK010000000
VERSION     DATRUK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42008
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.012"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            170..1624
                     /locus_tag="VBE34_000567"
     CDS             170..1624
                     /locus_tag="VBE34_000567"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEO7263307.1"
                     /translation="MANKIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGDFVIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPETHENYRQSERLELYQRYIDQLLAEGKA
                     YKSYVTEEELAAERERQELAGETPRYINEFIGMSETEKEAYIAEREAAGIIPTVRLAV
                     NESGIYKWTDMVKGDIEFEGSNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPQFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     SEAVFNFIALLGWNPGGEEEIFSREQLINLFDENRLSKSPAAFDQKKMDWMSNDYLKN
                     ADFESVFALCKPFLEEAGRLTDKAEKLVELYKPQLKSADEIVPLTDLFFADFPELTEA
                     EKEVMAAETVPTVLSAFKEKLVSLSDEEFTRDTIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIYLLGKEKSVQHIDNMLAKL"
     gene            complement(1664..2632)
                     /locus_tag="VBE34_000568"
     CDS             complement(1664..2632)
                     /locus_tag="VBE34_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEO7263308.1"
                     /translation="MKFGKKLGFLALLMSIVLILGACGKTGLGNSSGNSTKNVTKKSA
                     KDLKLGVSISTTNNPYFVAMKDGIDKYASNKKISIKVADAQDDAARQADDVQNFISQN
                     VDAILINPVDSKAIVTAIKSANNANIPVILMDRGSEGGKVLTTVASDNVAAGKMAADY
                     AVKKLGKKAKAFELSGVPGASATVDRGKGFHSVAKSKLDILSSQSANFDRAKALNTTQ
                     NMIQGHKDVQIIFAQNDEMALGAAQAVKSAGLQNVLIVGIDGQPDAHDAIKKGDISAT
                     IAQQPAKMGEIAIQAAIDHYKGKKVEKETISPIYLVTKDNVEKYNW"
     gene            complement(2685..3626)
                     /gene="rbsC"
                     /locus_tag="VBE34_000569"
     CDS             complement(2685..3626)
                     /gene="rbsC"
                     /locus_tag="VBE34_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771495.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HEO7263309.1"
                     /translation="MKNMMKYISELTTLIALAGLMVIITLINPNFLTTNNLLNLLLQV
                     TANGFIAFGMTFVILTGGIDLSVGSILALSSALTAGLIGHGVPVGIAIVLSVALGGIL
                     GMLNGLFISYGKLTPFIVTLATMTIFRGATLVYSNGNPITAGLSDSFLFQFLGQGYIV
                     GIPFPVILMFLTFIILYILLHKTAFGKSVYALGGNEKAAYISGIKLNKVKIIIYTISG
                     IMASISGLIITSRLSSAQPTAGASYEMDAIAAVVLGGTSLSGGKGRIIGTLIGALIIG
                     VLNNGLNIIGVSAFWQQVVKGIVILMAVLLDRFKVAK"
     gene            complement(3628..5106)
                     /locus_tag="VBE34_000570"
     CDS             complement(3628..5106)
                     /locus_tag="VBE34_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEO7263310.1"
                     /translation="MKIDMRNISKSFGTNKVLEKIDLELQSGQIHALMGENGAGKSTL
                     MNILTGLFPASTGTIYIDGEERTFSNPQEAEEFGISFIHQEMNTWPEMTVLENLFLGR
                     EIKTTFGLLNQKLMRQKALEAFKRLGVTIPLDIPIGNLSVGQQQMIEIAKSLLNQLSI
                     LVMDEPTAALTDRETENLFRVIRGLKQEGVGVVYISHRMEEIFKITDFVTVMRDGVIV
                     DTKETSLTNSDELVKKMVGRKLEDYYPEKHSEIGPVAFEVSNLCGDNFEDVSFYVRKG
                     EILGFSGLMGAGRTEVMRTIFGIDKKKSGKVKIDNQEITITSPSQAIKQGIGFLTENR
                     KDEGLILDFNIKDNMTLPSTKDFSKHGFFDEKTSTTFVQQLINRLYIKSGRPDLEVGN
                     LSGGNQQKVVLAKWIGIAPKVLILDEPTRGVDVGAKREIYQLMNELADRGVPIVMVSS
                     DLPEILGVSDRIMVMHEGRISGELSRKEADQEKVMQLATGGK"
     gene            complement(5122..5520)
                     /gene="rbsD"
                     /locus_tag="VBE34_000571"
     CDS             complement(5122..5520)
                     /gene="rbsD"
                     /locus_tag="VBE34_000571"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEO7263311.1"
                     /translation="MKKTGILNSHLAKLADDLGHTDQVCIGDLGLPVPNGIPKIDLSL
                     TSGIPSFQEVLDIYLENILVEKVILAEEIKEANSDQLSRLLAKLDNSVNIEYVSHNHL
                     KQMTQDVKAVIRTGENTPYSNIILQSGVII"
     gene            complement(5495..6406)
                     /gene="rbsK"
                     /locus_tag="VBE34_000572"
     CDS             complement(5495..6406)
                     /gene="rbsK"
                     /locus_tag="VBE34_000572"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEO7263312.1"
                     /translation="MSNIVIIGSISMDLVMETNRIAKEGETVFGQRFSMVPGGKGANQ
                     AVAIGRLSQDRDNITILGAIGEDSFGPILLDNLNKNHVTTDFVGTIPSSSGVAQITLY
                     NNDNRIIYCPGANGKVDTKKWSQEWSIIKEADLVVLQNEIPHQANMKIANFCKEHSIK
                     LLYNPAPSRKTDIEMLDKVDYFTPNEHECQELFPNQKLEDILATYPEKLIVTLGTKGA
                     IYSDGKESHLIPALETKAVDTTGAGDTFNGAFGYAISKKFKIANALRFATLAAHLSVQ
                     KFGAQGGMPTIKEMEDNQHYEKDWYIK"
     gene            complement(6399..7385)
                     /locus_tag="VBE34_000573"
     CDS             complement(6399..7385)
                     /locus_tag="VBE34_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7263313.1"
                     /translation="MSTIRQVAEKAGVSTSTVSRYISQNGYVSQKASQKIEQAIRELH
                     YVPNFLAQSLKTKKNQLVGLLLPDISNPFFPRLARGVEEFLKEQGYRVMLGNTNNKSH
                     LEEEYLNVLLQSNAAGIITTHDFTKNHPEIDIPVVVVDRVNQETQYGVFSDNKEGGKL
                     AAQAIWTAGATNILLIRGPLDKADNLNQRFQGSQNYLLNKGARFAIEDSASFDFAEIQ
                     IEAKTLLDHHPDIDSIIAPSDIHAIAYLHEILNRGKRIPEDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYIMGQKAAELIFKITNQLPITNKRIKLPVHYVERETLRRKIDE"
     gene            7567..8655
                     /locus_tag="VBE34_000574"
     CDS             7567..8655
                     /locus_tag="VBE34_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7263314.1"
                     /translation="MSHAWKELTFYKKKYLLIELLIIVMMFMVVFLSGLANGLGRAVS
                     AAIENNPAQTYILNEGAEQVITSSVLTTKDQTDLNSLNLKDSTTLNIQRSSLTRQGHE
                     KKIDISYFAIDKDSFMAPTLSEGKQLTSYKKAIILNDSLKAEGIKLGDKVIDKSSSIS
                     LTVVGFVHNSMYGHGPVAFIDKDIYTEINKKINPQYQFLPQALVMKNDKSISHLPTQL
                     EAVSKKDVIQHIPGYSAEQSTLNMILWVLVVASAGILGVFFYIITLQKRHEFSVMKAI
                     GTKMSEIALFQLSQVIILALFGIIVGDRLAIALSYVLPAQMPFVINWQNIILVSFVFL
                     VIAMISSALSIVKVAKIDPVEVINGGGE"
     gene            8655..9341
                     /locus_tag="VBE34_000575"
     CDS             8655..9341
                     /locus_tag="VBE34_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7263315.1"
                     /translation="MSAILELKHISKHYPDGDELLSILDNLDLSVSAGEFVAILGPSG
                     SGKSTLLSIAGLLLGADQGSLYVNHENVTDLSQRQRTQLRREALGFIFQSHQLLPYLT
                     IQEQLQQEARFAKHYDKKTSLEEINKLLSDLGIEQCAHKYPNQLSGGQKQRAAIARAF
                     INHPKVILADEPTASLDEERGRQVTELIRQEVKSHNTAAIMVTHDERVLDLVDTVYRL
                     KDGKLVKENF"
     gene            9372..10043
                     /locus_tag="VBE34_000576"
     CDS             9372..10043
                     /locus_tag="VBE34_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7263316.1"
                     /translation="MIKILVVEDNIVQQKIITTKLTQEGYQFITASNGQEALNCLDTE
                     EVQLIITDIMMPMMDGYQLIQELRSAAYNVPIIVMTAKSQMEDMTKGFGLGADDYMVK
                     PVQLQELALRIKALLRRANIVAQHQLIIGNTCLNEDELSLKYFEQEIVFPQKEFRVLF
                     HLLSYPNRIFTRLELLDSIWGMDTDLDERVVDACINKIRRKVEHLPDFKIETVRGVGY
                     RAKND"
     gene            10027..11106
                     /locus_tag="VBE34_000577"
     CDS             10027..11106
                     /locus_tag="VBE34_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7263317.1"
                     /translation="MQKMTKLRRFRFPLRFYFTLMFVLTMLFSVLASLLLVAAIVFTF
                     FQGVLTTHVLQVSALAVVFLSLVIASISMWYGSYHLTKPILDISHIVSNVAGGDFEGH
                     IYRNSNRRKSYEYYNELDELSESINQMIVSLSHMDHMRKDFITNVSHELKTPIAAVAN
                     IVELLQDPELDEETQSELLGLVKTESLRLTRLCDTMLQMSRVDNQETIGELSSVRVDE
                     QIRQAMISLTERWQAKRINFQLDSKPYTVYSNSDLLMQVWINLLDNAIKYSEDIVDLS
                     VRMEETNNHYLRVIISDKGRGISQYDVQHIFDKFYQADQSHNQQGNGLGLAIVKRIIV
                     LCKGHISVSSQLEIGTEFCVELPLS"
     gene            11260..12450
                     /locus_tag="VBE34_000578"
     CDS             11260..12450
                     /locus_tag="VBE34_000578"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEO7263318.1"
                     /translation="MGKEKLILAYSGGLDTSVAIAWLKKDYDVIAVCMDVGEGKDLDF
                     IHDKALTIGAIESYILDVKDEFAEHFVLPALQAHAMYEQKYPLVSALSRPIIAQKLVE
                     MAHQTGATTIAHGCTGKGNDQVRFEVAIAALDPELKVIAPVREWQWHREEEITFAKAN
                     GVPIPADLDNPYSIDQNLWGRANECGVLENPWNQAPEEAFGITKSPEEAPDCAEYIDI
                     TFQNGKPIAINNQEMTLADLILSLNEIAGKHGIGRIDHVENRLVGIKSREIYECPAAM
                     VLLAAHKEIEDLTLVREVSHFKPILENELSNLIYNALWFSPATKAIIAYVKETQKVVN
                     GTTKVKLYKGSAKVVARHSSNSLYDENLATYTAADNFDQDAAVGFIKLWGLPTQVNAQ
                     VNKG"
     gene            12469..13857
                     /gene="argH"
                     /locus_tag="VBE34_000579"
     CDS             12469..13857
                     /gene="argH"
                     /locus_tag="VBE34_000579"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEO7263319.1"
                     /translation="MTKNHKLWGGRFESSLEKWVEEFGASISFDQKLAPYDMKASMAH
                     VTMLGKTDIISQEEAGLIKDGLKILQDKYRAGQLTFSISNEDIHMNIESLLTAEIGEV
                     AGKLHTARSRNDQVATDMHLYLKDKLQEMMKKLLHLRTTLVNLAENHIYTVMPGYTHL
                     QHAQPISFGHHLMAYYNMFTRDTERLEFNMKHTNLSPLGAAALAGTTFPIDRHMTTRL
                     LDFEKPYSNSLDAVSDRDFIIEFLSNASILMMHLSRFCEEIINWCSYEYQFITLSDTF
                     STGSSIMPQKKNPDMAELIRGKTGRVYGNLFSLLTVMKSLPLAYNKDLQEDKEGMFDS
                     VETVSIAIEIMANMLEKMTVNEHIMMTSTETDFSNATELADYLASKGVPFRKAHEIVG
                     KLVLECSKNGSYLQDIPLKYYQEISELIENDIYEILTAKTAVKRRNSLGGTGFDQVKE
                     QILLARKELKAE"
     gene            13997..14878
                     /locus_tag="VBE34_000580"
     CDS             13997..14878
                     /locus_tag="VBE34_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEO7263320.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKLCKQLIETLVESMGITVPVAIHLDHGHYDDALECIEVGYTS
                     IMFDGSHLPVEENLEKAREVVAKAHAKGISVEAEVGTIGGEEDGIVGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPANWEGLDLDHLKKLTEAVPGFPIVLHGGSGIPDDQI
                     QEAIKLGVAKVNVNTECQLAFCQATRDYAVEFNANEAEYMKKKLFDPRKFLKPGFDAI
                     TEAVEERIDVFGSANKA"
     gene            complement(14963..15880)
                     /locus_tag="VBE34_000581"
     CDS             complement(14963..15880)
                     /locus_tag="VBE34_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO7263321.1"
                     /translation="MTRKIGIIGMGHVGSTVAHALIAQGIGDDYVFIDTNVDKVNADM
                     IDFQDALANATYHANIVLNDYDALSDADVVISALGDISLQNNKEANRFAELPFTSKEV
                     YDVSQKLKSSGFRGILIVVTNPVDAITQLYQEYTQFPKSRVIGTGTLLDTARMKRVLG
                     QRLQVDPRSIGGYNLGEHGNSQFVAWSQVTVKGQPAISLLKVEELEQIKEASLKGGHT
                     VFFGKKYTSYGIATAAVRIVLAVLNDSHELLPVSTFYESEGVYLGYPAIIGRVGIVER
                     ISLSLSPKEEQQLTDSAAFIRNYLKHINL"
     gene            16119..16307
                     /locus_tag="VBE34_000582"
     CDS             16119..16307
                     /locus_tag="VBE34_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEO7263322.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTVLIDGKP
                     KKVWVSARALKSGKVERV"
     gene            16439..16804
                     /locus_tag="VBE34_000583"
     CDS             16439..16804
                     /locus_tag="VBE34_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7263323.1"
                     /translation="MTVKINTRDGQIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQALLRKENYAKGVVVRSSEAGIAVDVYTVMSYGVKISEVSKNIQERVKFNLENQLG
                     LSADMVNVYVQSIKVVGED"
     gene            16804..18468
                     /locus_tag="VBE34_000584"
     CDS             16804..18468
                     /locus_tag="VBE34_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEO7263324.1"
                     /translation="MSKITTSLFQEMVQAASTRLSKQAEYVNSLNVFPVPDGDTGTNM
                     GMTMENGAKEVSDKPATTVGEVGQILSKGVLMGARGNSGVITSQLFRGFGQSIKDKEE
                     LTGQDLAHAFQNGVEVAYKAVMKPVEGTILTVSRGAATAALKKAEETDDAVEVMRATL
                     KGAKRALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFKATPATM
                     TEMVNAEHHKAVVGHVATEDIKYGYCTEIMVGLKQGPTYVKEFNYEEFQGYLSNLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVENMRNQHDAQMQKVEVEETVK
                     ETKEYGIIAVVAGDGLAEIFKSQGVDYIISGGQTMNPSTEDIVKAIEKVNARNVIILP
                     NNKNIFMAAQSAADVVDIPAAVVETRTVPQGFTSLLAFDPAKSLETNVADMTNSLSDV
                     ISGSVTLAVRDTTIDGLEIHENDILGMVDGKILVSTPDMEKALKDTFDKMIDEDSEIV
                     TIYVGEDGKQALAETLSEYLEETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            18616..19500
                     /locus_tag="VBE34_000585"
     CDS             18616..19500
                     /locus_tag="VBE34_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEO7263325.1"
                     /translation="MSLIILTVILVLVIVLLITSLYVVKQQTVAIIERFGKYQKTATS
                     GIHIRVPLGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNIATQYRVNENNVTDAYY
                     KLIKPEAQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIVKT
                     LITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVGIA
                     QQRKAIVDGLADSIQELKDANVTLTEEQIMSILLTNQYLDTLNTFAINGNQTIFLPNN
                     PEGVEDIRTQVLSALKTR"
     gene            complement(19592..19708)
                     /locus_tag="VBE34_000586"
     CDS             complement(19592..19708)
                     /locus_tag="VBE34_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEO7263326.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
     gene            complement(20076..20306)
                     /locus_tag="VBE34_000587"
     CDS             complement(20076..20306)
                     /locus_tag="VBE34_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263327.1"
                     /translation="MKLKISVLTAASAVTLLLATSASLAKADTVSYPSRDAGFIYSRG
                     YAPSESSYPSRDAGFIYSRGYAPKHFLSAYDK"
     gene            complement(20648..21388)
                     /locus_tag="VBE34_000588"
     CDS             complement(20648..21388)
                     /locus_tag="VBE34_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7263328.1"
                     /translation="MTQAILEIKHLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STFLRSINLLEEPSGGEILYHGHNVLEKGYDLNNYREKLGMVFQSFNLFENLNILENA
                     IVAQTTVLKRERQEAEKIAKENLNAVGMTEQYWKAKPKQLSGGQKQRVAIARALSVNP
                     EAILFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFAKEVSDRVIFMDKGI
                     IAEQGTPKQLFENPTQERTKEFLQRFLK"
     gene            complement(21398..22948)
                     /locus_tag="VBE34_000589"
     CDS             complement(21398..22948)
                     /locus_tag="VBE34_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO7263329.1"
                     /translation="MKKILLSLFTALLITFGGMTSIQADEYLRVGMEAAYAPFNWTQN
                     DNTNGAVPIEGTDQYANGYDVQVAKKLAKKLNKKVVVVKTKWEGLVPALTSGKLDMII
                     AGMSPTEERKKEINFSKPYYISEPTLVVNAEGKYTNAKNISDFKNAKVTAQQGVYLYN
                     LIDQINGVKKEVAMGDFNQLRQAVESGVVDAYVSERPDATSAQTANPKLKMIELHQGF
                     KTSDADTNISVGMRKGDNRINQVNQVLESISRDKQIALMDKMIKEQPSVKKEKNGKPN
                     FFEQMATILKNNGSQFLRGTATTLLISMVGTIVGLFIGLLIGVFRTAPKSDNKLKAAL
                     QKLLGWLLNIYIEVFRGTPMIVQSMVIYYGTAQAFGVSLDRTLAAIFIVSINTGAYMS
                     EIVRGGIFSVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFILTFTVTRILRYIEKRFDSDNYT
                     TGANQLQV"
     gene            23084..24967
                     /locus_tag="VBE34_000590"
     CDS             23084..24967
                     /locus_tag="VBE34_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEO7263330.1"
                     /translation="MKKCFLAICLALSFFMVSVQADEVDYNIPHYEGNLTIHNDNSAD
                     FTEKVTYQFDSSYNGQYVTLGTAGKLPDNFDINNKPQVEVSINGKVRKVSYQIEDLED
                     GYRLKVFNGGEAGDTVKVNVQWKLKNVLFMHKDVGELNWIPISDWDKTLEKVDFWIST
                     DKKVALSRLWGHLGYLKTPPKIRQNNNRYHLTAFNVNKRLEFHGYWDRSYFNLPTNSK
                     NNYKKKIEHQEKIIERHGFILSFLLRILLPSFFIIVTLFISIRVFLFRKKVNKYGQFP
                     KDHHLYEAPEDLSPLELTQSIYSMSFKNFQDEEKKTHLISQEQLIQSILLDLIDRKVL
                     NYDDNLLSLANLDRASDAEIDFIEFAFADSTSLKPDQLFSNYQFSYKETLRELKKQHK
                     ASDLQNQMRRRGSNALSRITRLTRLISKDNINSLRRKGISSPYRKMSSEESKELSRLK
                     RFSYLSPLISFVVIIYTLFLNYFTYFCIYLLLFGVILLLNKIIFMMTRKISNGYIVTE
                     DGASRVYQWTSFRNMLRDIKSFDRSELESIVLWNRILVYATLFGYADRVEKVLRVNQI
                     DIPERFANIDSHRFAISVNQSSNHFSTITEDVSHASNFSVNSGGSSGGFSGGGGGGGG
                     GAF"
     gene            25013..25852
                     /locus_tag="VBE34_000591"
     CDS             25013..25852
                     /locus_tag="VBE34_000591"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEO7263331.1"
                     /translation="MLIIELLKALFLGVVEGVTEWLPVSSTGHLILVQEFMKLNQSKS
                     FVEMFNIVIQLGAIMAVIVIYFKRLNPFQPGKSAREIRLTWQLWLKVVIACIPSILIA
                     LPFDNWFEAHFNFMIPIAIALIFYGFVFIWVEKRNAHLKPQVTELASMSYKTAFLIGC
                     FQVLSIVPGTSRSGATILGAIIIGTSRSVAADFTFFLAIPTMFGYSGLKAVKYFLDGN
                     VLSLDQSLILLVASLTAFVVSLYVIRFLTDYVKRHDFTIFGKYRIVLGSLLILYWLVV
                     HLF"
     gene            25972..26727
                     /gene="mecA"
                     /locus_tag="VBE34_000592"
     CDS             25972..26727
                     /gene="mecA"
                     /locus_tag="VBE34_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEO7263332.1"
                     /translation="MEMKQISETTLKITISMEDLEDRGMELKDFLIPQEKTEEFFYSV
                     MDELDLPENFKNSGMLSFRVTPKKDRIDVFVTKSELSKDLNLEELADLGDISKMSPED
                     FFKTLEQSMLEKGDTDAHAKLAEIENMMDKATQEVVEENVSEEQPEKEVETIGYVHYV
                     FDFDNIEAVVRFSQTIDFPIEASELYKNGKGYHMTILLDLENQPSYFANLMYARMLEH
                     ANVGTKTRAYLKEHSIQLIHDDAISKLQMIEMG"
     gene            26729..27889
                     /locus_tag="VBE34_000593"
     CDS             26729..27889
                     /locus_tag="VBE34_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEO7263333.1"
                     /translation="MIPFTIEYIFVLIGAFLLSIILTPIIRVISLKVGAVDKPNARRI
                     NKVPMPSSGGLAIFLSFVVTTLFFMPMAASRHFIEVSYFHYILPVIIGGLVVTTTGFI
                     DDIFELRPRYKMLGIIIAAIIIWKFTHFRFDSFKIPIGGPLLEFGPILTFFLTVLWII
                     SITNAINLIDGLDGLVSGVSIISLATMAVVSYFFLPKIDFFLTLTIVILIASIVGFFP
                     YNYHPAIIYLGDAGALFIGFMIGVLSLQGLKNSTAVAVITPVIILGVPILDTAVAIVR
                     RKLSGKKISEADKMHLHHRLLSMGFTHRGAVLVVYGIAIIFSLIALLLNVSSRIGGIF
                     LLLALLLVMEIFIEGLNIWGENRTPLFNLLKFIGNSDYRQSVIAKYSDKHQK"
     gene            28054..28824
                     /gene="sufC"
                     /locus_tag="VBE34_000594"
     CDS             28054..28824
                     /gene="sufC"
                     /locus_tag="VBE34_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEO7263334.1"
                     /translation="MSVLEIKNLHVSIEDKEILKGLNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTAGEILFDGEDILELEVDERARLGLFLAMQYPSEVPGITNAEFIR
                     AAMNAGKADDDKISIRQFITKLDEKMELLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGEGFGAMIITHYQRLLNYITPDKVHV
                     MMDGKVVLSGGPELAVRLEKEGYAQIAEELGLEYKEEV"
     gene            28861..30123
                     /gene="sufD"
                     /locus_tag="VBE34_000595"
     CDS             28861..30123
                     /gene="sufD"
                     /locus_tag="VBE34_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEO7263335.1"
                     /translation="MSKEAILNFLQAKGEPTWLQELRLKAFEKIEELELPVIERVKFH
                     RWNLGDGTILENDYTANIPDFTELGNNPKLVQIGTQTVLEQVPMELIEKGVVFTDFYS
                     ALEEIPEVIERYFGKARPFEEDRLAAYHTAYFNSGAVLYIPDNVEITQPIEGLFYQDS
                     QSKVPFNKHILLIVGKNAKVSYLERFESIGDGTERTSANISVEVIAQAGSQIKFASID
                     RLGENVTTFISRRGRHSSDATIDWALGVMNEGNVVADFDSDLIGDGSHANLKVVAASS
                     GRQVQGIDTRVTNYGCNSVGHILQHGVILERGTLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDKARSDANPILLIDENDVTAGHAASIGQVDPEDLYYLMSRGLNQKTAEQLVIRGF
                     LGTVIAEIPVKEVRDEMIAVIDTKLEKR"
     gene            30125..31357
                     /locus_tag="VBE34_000596"
     CDS             30125..31357
                     /locus_tag="VBE34_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO7263336.1"
                     /translation="MTLLDSYKLKQDFPILNQLVNDEPLIYLDNAATTQKPNQVLETL
                     RDYYQNDNANVHRGVHTLAERATAQYETAREKARQFLNAKSSKEILFTRGTTTGLNWV
                     AKFAESILERGDEVLISIMEHHSNIIPWQQACERTGAKLVYAYLKDGSLDLEDFYNKL
                     SSKTKFVSLAHISNVLGCITPVKAIAERVHQVGAYMVVDGAQSAPHMAIDVQDLDCDF
                     FALSGHKMLGPTGIGVLYGKESILDKMPPVEFGGEMIDFVYEHSATWKELPWKFEAGT
                     PNIAGAIAFGEALDYLTDVGMDEIHQYEQSLVSYVLPKLQAIDGLTIYGPSDAESHVG
                     VIAFNLEGLHPHDVATAMDYEGVAVRAGHHCAQPLINHLGIHSAVRASFYFYNTKEDC
                     DKLVDAIQKTKEFFNGTL"
     gene            31344..31787
                     /locus_tag="VBE34_000597"
     CDS             31344..31787
                     /locus_tag="VBE34_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEO7263337.1"
                     /translation="MALSKLDNLYMAVVADHSKHPHHHGFLEGVEQVQLNNPTCGDVI
                     SLSVKFDGNIISDIAFAGNGCTISTASSSMMTDAVIGKTKEEALQLADVFSKMVQGNQ
                     NPKQEKLGDAEFLAGVSKFPQRIKCATLSWNALRKAIERDNQAEL"
     gene            31887..33305
                     /gene="sufB"
                     /locus_tag="VBE34_000598"
     CDS             31887..33305
                     /gene="sufB"
                     /locus_tag="VBE34_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEO7263338.1"
                     /translation="MSEINEKVEPQPIDLGEYKFGFHDDVKPIYSTGKGLNEAVIREL
                     SAAKGEPEWMLDFRLKSLETFNKMPMQTWGADLSDIDFDDIIYYQKASDKPARDWDDV
                     PEKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEYDKLGIVFTDTDSALKEY
                     PELFKKYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRATAKKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANKGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKDSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSEAEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(33376..34563)
                     /locus_tag="VBE34_000599"
     CDS             complement(33376..34563)
                     /locus_tag="VBE34_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO7263339.1"
                     /translation="MPKLIVSFLCILLSLTCVNSVQAEEHKDIMQITREAGYDVKDIN
                     KPKASIVIDNKGHILWEDNADLERDPASMSKMFTLYLLFEDLAKGKTNLNTTVTATET
                     DQAISKIYEISNNNIHAGVAYPIRELITMTAVPSSNVATIMIANHLSQNNPDAFIKRI
                     NETAKKLGMTKTHFYNPSGAVASAFNGLYSPKEYDNNATNVTTARDLSILTYHFLKKY
                     PDILNYTKYPEVKAMVGTPYEETFTTYNYSTPGAKLGLEGVDGLKTGSSPSAAFNALV
                     TAKRQNTRLITVVLGVGDWSDQDGEYYRHPFVNALVEKGFKDAKNISSKTPVLKAVKP
                     KKEVTKTKTKSIQEQPQTKEQWWTKTDQFIQSHFVSILIVLGTIASLCLLAGIVLLIK
                     RSR"
     gene            complement(34716..35951)
                     /locus_tag="VBE34_000600"
     CDS             complement(34716..35951)
                     /locus_tag="VBE34_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO7263340.1"
                     /translation="MKKIITSILLLSCIFFMPTISAESFNASAKHALAVDLDSGKILY
                     EKDANKPAAIASLTKIMTVYMVYKEIDNGNLKWNTKVNISDYPYQLTRESDASNVPLE
                     KRRYTVKQLVDAAMISSANSAAIALAEHISGTESKFVDKMTAQLEKWGIHDSHLVNAS
                     GLNNSMLGNHIYPKSSQNDENKMSARDIAIAAYHLVNEYPSILKITSKSVAKFDKDIM
                     HSYNYMLPDMPVFRPGITGLKTGTTELAGQSFIATSTESGMRLLTVIMHADKADKDKY
                     ARFTATNSLLNYITNTYEPNLVLAKGAAYKGKEASVRDGKEQSVIAVAKNDLKVVQKK
                     NITKQNQLKINFKKELTAPITKKENLGKAYYVDLNKVGKGYLIKEPSVHLVAKDSIER
                     SFFLKVWWNHFVRYVNEKL"
     gene            36193..37851
                     /locus_tag="VBE34_000601"
     CDS             36193..37851
                     /locus_tag="VBE34_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7263341.1"
                     /translation="MAKQKNNWRRVGVGVLTLASVATLAACGSKSASQDSNGAINWAI
                     PTEINTLDLSKVTDTYSNLAIGNSSSNFLRLDKDGKTRPDLATKVDVSKDGLTYTATL
                     RKGLKWSDGSKLTAKDFVYSWQRLVDPKTASQYAYLAVEGHVLNADKINEGQEKDLNK
                     LGVKAEGDDKVVITLSSPSPQFIYYLAFTNFMPQKQEVVEKYGKDYATTSKNTVYSGP
                     YTVEGWNGSNGTFTLKKNKNYWDAKNVKTKEVRIQTVKKPDTAVQMYKRGELDAANIS
                     NTSAIYQANKNNKDVTDVLEATTAYMEYNTTGSVKGLDNVKIRRALNLATNRKGVVQA
                     AVDTGSKPAIAFAPTGLAKTPDGTDLAKYVAPGYEYNKTEAAKLFKEGLAESGLTKLK
                     LTITADADAPAAKNSVDYIKSTWEAALPGLTVEEKFVTFKQRLEDSRKQNFDIVVSLW
                     GGDYPEGSTFYGLFKSDSQNNDGKFANKDYDAAYNKAISEDAMKPAESAKDYKEAEKI
                     LFEQGAYNPLYFRSGKGLQNPKLKGVIRNTTGLSIDFTHAYKNN"
     gene            37966..38880
                     /locus_tag="VBE34_000602"
     CDS             37966..38880
                     /locus_tag="VBE34_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7263342.1"
                     /translation="MIKYILKRVAILLVTLWVVITLSFFLMQILPGTPYNNPKLTEEM
                     ITLLNKQYGLDKPVWQQYLTYLWNVLHGDFGTSYQSVNQPVSRMISLRLGVSVHLGVQ
                     ALVFGVLGGILVGAISARHKNDKVDGILSVIATLGISMPSFIIGILLLDYFGFKWNLL
                     PLSGWGTFSQTILPSLALGLPTLASVSRFFRSEMIETLNSDYVQLARSKGMTIRQVTR
                     KHAYRNSMIPILTLIGPLAAGLLTGSALIEQIFSIPGIGQQFVTSIPTKDYPVIMGTT
                     IVYAVMLMVAILITDVVISIVDPRVRLQ"
     gene            38890..39921
                     /locus_tag="VBE34_000603"
     CDS             38890..39921
                     /locus_tag="VBE34_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7263343.1"
                     /translation="MADKNRTFKLVGAGSSSTQEKIEKPALSFMQDAWRRLKKNKLAV
                     VSLYLLALLLTFSLASNLFVTQKDANGFDSKKVTTYRNLPPKLSSNLPFWNGSIKYAG
                     NTESTDAYKSQNVPEKVKYALGTDSLGRSVAKRIIVGIRISLLVAIAATFIDLIIGVT
                     YGLVSGFAGGRLDTLMQRVVEVISSIPNLVIVTMLGLVLGNGITAIIISIAFTGWTSM
                     SRQVRNLTLSYREREFVLAARSLGESPIKIAFKHILPNISGIIIVQIMMTIPSAIMYE
                     AVLSAINLGVKPPTASLGSLISDAQENLQYYPYQVILPALALVMISLAFILLGDGLRD
                     AFDPKSSDE"
     gene            39934..40983
                     /locus_tag="VBE34_000604"
     CDS             39934..40983
                     /locus_tag="VBE34_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7263344.1"
                     /translation="MEKETILSVNNLHVDFHTYAGEVKAIRDVNFELKKGETLAIVGE
                     SGSGKSVTTRTLIGLNAKNSEISGNVQFKGRNLVELSEEEWTKVRGNEISMIFQDPMT
                     SLDPTMKIGMQIAEPMMIHQKISKKDALKLALELMKDVGIPNAEEHINDYPHQWSGGM
                     RQRAVIAIALAADPEILIADEPTTALDVTIQAQILNLMKKIQAERDSSIVFITHDLGV
                     VAGMADRVAVMYAGKIVEFGTVDEVFYNPQHPYTWGLLNSMPTTETESGSLESIPGTP
                     PDLLNPPKGDAFAARNEFALDIDHEEEPPYFKVSETHFAATWLLDERSPKVLPPLPIQ
                     KRWEKWNEIEGRKAQ"
     gene            40980..41912
                     /locus_tag="VBE34_000605"
     CDS             40980..41912
                     /locus_tag="VBE34_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7263345.1"
                     /translation="MTENRKKLVEVKNVSLTFNKGKANEVRAIDNVSFDIYEGEVFGL
                     VGESGSGKTTVGRSILKLYDISDGEITFNGEVISHLKGKALHSFRKDAQMIFQDPQAS
                     LNGRMKIRDIVAEGLDIHKLAKSKSDRDSKVQALLDLVGLNKDHLTRYPHEFSGGQRQ
                     RIGIARALAVEPKFIIADEPISALDVSIQAQVVNLMQKLQREQGLTYLFIAHDLSMVK
                     YISDRIGVMHWGKLLEVGTSDDVYNKPIHPYTKSLLSAIPEPDPESERQRVHQPYNPA
                     IEQDGQERQMHEITPGHFVLSTPQEAEEYKKQIL"
BASE COUNT        13534 a         6902 c         8178 g        13394 t
ORIGIN      
        1 tttatcgaaa attagtgtga ctattattac tgattatcat ttttagtgat ttagtggtat
       61 aatagggttg attggcaatc atctgtaaga gtttgaatga tgttttgccc ccttgactaa
      121 ttcagttaaa ctgaaaaagt ttactatcag aaaatatagg agtagaattt tggctaataa
      181 gattcgtgtg cgttatgcac caagtccaac aggattgtta cacatcggaa atgcgcgtac
      241 tgcgctcttt aactaccttt atgctcgcca tcatggtggg gattttgtta ttcgtattga
      301 agatactgac cgaaaacgtc acgtcgaaga tggtgaacgc tctcaattgg agaacttacg
      361 ttggctaggt atggactggg atgaaagccc tgaaacacat gaaaattatc gtcaatcaga
      421 acgccttgag ctctaccaac gttatattga tcagttgtta gcagaaggta aagcctacaa
      481 gtcctatgtg actgaggaag aattagcagc agagcgtgaa cgccaagaat tggcaggcga
      541 aacacctcgc tatatcaacg aatttatcgg aatgtcagaa actgaaaaag aagcttacat
      601 tgcagaacgt gaagcagcag gcatcattcc gacagtacgc cttgctgtta atgaatctgg
      661 gatttataag tggactgata tggtaaaagg tgacattgag tttgaaggct ctaatatcgg
      721 cggagactgg gttattcaga agaaagatgg ttatccaact tacaatttcg cagtagtcat
      781 tgatgatcat gacatgcaaa tttcgcatgt tattcgtggc gatgaccata ttgcaaatac
      841 gccaaaacaa ttaatggttt atgaagcact cggttgggaa gcgccacaat ttggtcacat
      901 gacattgatt ataaactcag agactggtaa gaaattatca aaacgcgaca caaatactct
      961 tcaatttatc gaagactatc gtaaaaaagg ttatatgtcg gaagctgtct ttaatttcat
     1021 tgcccttctt ggttggaatc ctggtggaga agaagaaatc ttctcacgcg agcaattaat
     1081 taatctattt gatgaaaatc gtcttagcaa atcaccagca gcattcgatc agaagaagat
     1141 ggattggatg agtaatgatt acctaaaaaa tgctgatttt gagagtgttt ttgcactttg
     1201 caaaccattc ttagaagaag ctggtcgact tacagacaaa gctgaaaaat tagttgagct
     1261 ttataaacct caattaaaat cggctgatga gattgtacca ttaacagacc ttttctttgc
     1321 agattttcca gaattaacag aagccgaaaa agaagtaatg gcagcagaaa cagtcccaac
     1381 agtcttatct gcttttaagg aaaagttagt atcgctttct gacgaagaat tcactagaga
     1441 tactattttc cctcaaatta aggctgttca aaaagaaaca ggcatcaaag gtaagaacct
     1501 atttatgcct attcgtattg cggtttctgg tgaaatgcat ggaccagagt taccagatac
     1561 tatttattta ttaggtaaag aaaaatcagt tcaacatatc gataatatgt tagcaaaatt
     1621 ataatataaa aagtcgtgaa ttttcacgac tttttgtgta cttttaccaa ttgtattttt
     1681 caacattgtc tttggtaaca aggtaaattg gagaaatcgt ttctttctct acttttttgc
     1741 ctttataatg atcaattgct gcttgaatag ctatttctcc catcttagct ggttgttgtg
     1801 caatagtggc tgaaatatca ccttttttaa tggcatcatg ggcatctggc tgaccatcaa
     1861 ttcctacgat aagaacattt tgcaagccag cagatttgac agcctgtgca gccccaagtg
     1921 ccatttcatc attttgtgcg aaaataattt ggacatcttt atgtccttga atcatatttt
     1981 gtgtagtatt caaagctttg gcgcggtcaa aattggcaga ttggcttgaa agaatgtcaa
     2041 gtttagattt tgcaactgaa tgaaatcctt taccacggtc tacagtagca gaggcacctg
     2101 gtactccaga taattcaaag gcttttgctt ttttgcctag ttttttaacg gcataatccg
     2161 cagccatctt accagcagca acgttatctg aagcgacagt agttaatact ttgcctccct
     2221 cactaccacg gtccattaga ataacaggaa tattagcgtt gtttgcagac ttaatagctg
     2281 ttacaatagc ttttgagtca acgggattaa tcaaaatagc atctacgttt tgactaataa
     2341 agttttgaac atcatctgct tgacgtgcgg cgtcatcttg agcatcagct accttaatgc
     2401 ttattttttt attgctcgca tatttgtcaa taccgtcttt catcgcaaca aagtaggggt
     2461 tgttagttgt tgaaatagat acacctagtt tcaaatcttt agcagatttt ttggttacat
     2521 tttttgtaga attcccagaa gaatttccaa gtccagtttt accacatgca ccgagaatga
     2581 ggacaataga catcaataag gctagaaagc caagtttttt tccaaatttc atttgaaact
     2641 cctttagata aaatataatg aacttataga aattagagta aagactattt tgctacttta
     2701 aagcgatcaa gtaaaacagc cattaaaata acgatacctt tcacaacttg ttgccaaaat
     2761 gcagagacac caatgatgtt taatccattg ttcaggacac caataatgag agcaccaatc
     2821 aatgttccaa tgatacgtcc tttcccacca gataaagacg ttccacctaa gacaacggcg
     2881 gcaatagcat ccatctcata acttgctcca gctgttggtt gtgctgagct aaggcgtgat
     2941 gtgataatta acccagaaat ggaagccatg atacctgaga tagtgtagat gataatttta
     3001 actttgttta acttaatacc cgagatatac gctgcttttt cattacctcc taaagcgtaa
     3061 acagatttcc cgaaggctgt tttatgtaac aaaatataca aaataataaa agtaagaaac
     3121 atgagaatga ctgggaatgg gataccaaca atatagcctt gtcccaaaaa ttggaataga
     3181 aaactatcgg ataatccagc ggtaattggg ttgccgtttg aataaactag cgttgctcct
     3241 ctaaagatag tcattgttgc cagtgtaacg ataaatggtg ttaattttcc gtaagaaatg
     3301 aagagtccgt ttagcattcc caagatgcca cctaaggcaa cagataaaac gatagcaata
     3361 ccaacgggta ctccgtggcc gattaatcca gctgttaatg cactagatag ggctaatatg
     3421 gacccgactg ataaatcaat accgccagtc aaaataacga aggtcatccc gaaagcgata
     3481 aaaccatttg cagttacttg taataataaa ttgagtagat tattggttgt gaggaaatta
     3541 ggattaatga gtgtgataat aaccattaaa ccagctaatg caatcagggt tgttaactct
     3601 gatatatatt tcatcatatt tttcacttta cttacctcct gtggctagct gcataacttt
     3661 ttcttggtca gcttcttttc gactgagttc tccactgatt cgaccttcgt gcattaccat
     3721 gatgcggtca ctaacaccta gaatttcagg taagtctgat gataccatga caattggaac
     3781 acctctgtct gctagttcat tcattaattg gtaaatctct cgtttagctc cgacatcaac
     3841 accacgtgtt ggctcatcaa gaattaagac tttaggagca ataccaatcc atttagcgag
     3901 aacgaccttt tgttggttac ctccagaaag atttccaacc tctaaatctg gtctccctga
     3961 tttaatgtag aggcgattaa tcaattgttg cacaaatgtt gtgcttgttt tttcatcaaa
     4021 gaaaccatgt ttgctaaaat ctttagtgct agggagagtc atattgtcct tgatattgaa
     4081 gtctaggatg agaccttcat cttttctatt ttcggtcaaa aaaccaatgc cctgcttgat
     4141 agcttggctt gggctagtaa tagtgatttc ttggttatca attttcacct taccagactt
     4201 ctttttatca attccaaaga tagttcgcat aacttcagta cgcccagctc ccattaatcc
     4261 agaaaatcca agaatctcgc ctttacggac ataaaaggaa acgtcctcga aattatctcc
     4321 acagaggtta ctaacttcaa aagcaacagg cccaatttca gaatgttttt caggatagta
     4381 atcttccaat tttcggccaa ccattttttt aaccaattcg tcagaatttg tgagtgatgt
     4441 ttcttttgta tctacaatga caccatctct catgacggtt acgaagtcag tgattttaaa
     4501 aatttcctcc attcgatggg agatatagac aacgcccact ccttcttgtt ttaaacctcg
     4561 gataactcgg aaaagatttt cagtttcacg atctgttaaa gcagcagtcg gttcatccat
     4621 taccaaaata gagagttggt ttaggagact tttggcgatt tcaatcattt gttgttgtcc
     4681 aacagacaag ttaccaatcg ggatgtctag gggaatagtc acacctaatc ttttaaaagc
     4741 ttcaagtgct ttttgtctca ttaatttttg attgagtaag ccaaaagttg tcttgatttc
     4801 acgtccaaga aagagatttt ccaagacagt catttccggc caagtattca tctcttggtg
     4861 aataaaacta ataccaaatt cctctgcttc ttgagggttt gaaaaagttc gttcctcgcc
     4921 atcaatataa atagttcctg tacttgctgg aaaaagtcct gttagaatgt tcattaaggt
     4981 tgatttaccc gcaccatttt cacccataag tgcgtgtatt tgaccagatt gtaattccaa
     5041 atcaatcttc tctagaactt tattagtacc gaaggatttg gagatattcc tcatgtcaat
     5101 tttcatggac taaactcctt tctagatgat aacaccagat tgtagaataa tatttgaata
     5161 cggtgtattt tcacctgtac gaataactgc tttgacatct tgggtcattt gttttaaatg
     5221 attgtgactg acgtactcta tgttaacaga gttatcaagt ttagctaata accttgataa
     5281 ttgatccgaa tttgcttcct tgatttcttc tgctaaaatg actttttcaa ctaagatatt
     5341 ctctaaatag atatcaagta cctcttggaa actaggtatt ccagaggtca aagacaaatc
     5401 aatctttgga atgccatttg gaacaggtag acccagatct ccaatacaaa cttgatcagt
     5461 atgcccaagg tcatctgcta atttagcgag atgactattt aatataccag tctttttcat
     5521 aatgttggtt gtcctccatt tctttaattg taggcatgcc accctgtgca ccaaattttt
     5581 gcacggataa gtgagcagct agagttgcaa aacgtaaggc attagcaatc ttaaattttt
     5641 tcgagatagc ataaccgaat gcaccgttaa aagtatcacc tgcaccagtt gtatcaacag
     5701 ctttagtttc taaggcagga attaaatggc tttcttttcc atcagagtag atggctcctt
     5761 ttgtgccgag tgttacaatt agtttttcag gatatgtggc taatatatcc tctagttttt
     5821 ggtttgggaa cagctcttga cattcgtgtt catttggtgt gaaataatcg accttatcga
     5881 gcatctcaat atcagtcttt ctagatggtg caggattata gagtaattta atactatgtt
     5941 ctttacaaaa attagcaatt ttcatatttg cttgatgagg aatttcattt tgtaatacaa
     6001 ctaaatcagc ttctttaatt attgaccact cctgagacca ctttttagta tcaacttttc
     6061 cgttggctcc aggacagtaa atgattcgat tatcattatt ataaagagtg atttgggcaa
     6121 cacctgaaga agatggtatc gttcccacaa aatccgtagt aacatggttt ttgtttaaat
     6181 tatccaaaag aatgggacca aaagaatcct cgccaatggc accaagaata gtaatattat
     6241 ctcgatcttg gctaagacgc ccaatagcga cagcctgatt agcaccttta cctcctggaa
     6301 ccattgaaaa acgttggcca aatacggttt ctccctcttt ggcgatacga ttggtttcca
     6361 taactaagtc catggaaata ctaccgataa tgacaatgtt actcatctat tttcctcctt
     6421 agggtttcac gttcgacata atgaacgggt aatttaatac gcttattggt aataggtaat
     6481 tgattagtaa ttttgaaaat taattcagct gctttttgtc ccataatata tgatgattgg
     6541 tgaatagttg acaacgaagg gtagataaat tggctcatta aaatatcatc ataaccaata
     6601 atttgtacat cttcagggat tcgcttccct cgattaagaa tctcgtgtag gtaggcaata
     6661 gcatgtatat cagatggagc gataatgcta tcaatatctg gatggtggtc aagtaaagtt
     6721 ttcgcttcaa tttgaatctc tgcaaaatca aaactggcac tatcttcgat agcaaaacga
     6781 gcccctttgt ttaataggta attttggctt ccttgaaacc tttgattaag gttatcagct
     6841 ttatctagcg gaccacgaat gagtaatata ttggtagcac ctgctgtcca aatagcttga
     6901 gctgctaatt ttcctccctc tttattatca gagaaaacgc cgtactgtgt ttcttgatta
     6961 acgcggtcaa caactacaac tggaatatct atttcaggat gatttttggt gaaatcgtgt
     7021 gttgtaataa tccctgcagc attactttgt aatagcacat ttaagtactc ttcttctaaa
     7081 tggctcttgt tatttgtgtt gccaagcatg acacgataac cctgttcttt tagaaattct
     7141 tcaactcctc tagcaagtct tggaaagaaa gggtttgaaa tatcgggaag tagtaatcca
     7201 acaagttgat tttttttggt tttcagagat tgtgcaagga aatttggaac ataatggagt
     7261 tcgcggatag cttgctcgat tttttgactt gctttttgac taacgtaacc attttgggat
     7321 atataccttg agacagtaga tgttgatacg ccagctttct cggcgacttg tctaatcgta
     7381 ctcatagaaa ctcctttctt ttattgtggg aacggtttca taattagaat tgtaatcgct
     7441 tactaaaaaa ttgtcaagct attttttaaa taatttaatg ataaaatatc attattttta
     7501 gaggtcatat tcaagtcata aagctgtgat actatgttca catcaaaaga tcaaaggaga
     7561 aaaaccatgt cacatgcttg gaaagaatta accttttata agaaaaaata cctcctgatt
     7621 gaattgctaa tcattgttat gatgttcatg gtagtttttt tatcaggttt agcaaacggt
     7681 ttaggtagag ctgttagtgc tgctattgaa aataacccag ctcaaaccta tattttgaat
     7741 gaaggtgctg aacaagtgat tacatcatct gtattgacaa ctaaagatca gacagattta
     7801 aacagtttga atttaaaaga tagtaccact ttaaatattc aacgttcgag tttaactcga
     7861 caaggacatg aaaagaaaat tgatatttct tattttgcta ttgataaaga ttcttttatg
     7921 gcacctacac tttctgaggg aaaacaactg acaagttaca aaaaagctat tattttaaat
     7981 gatagtttaa aagctgaagg gataaagtta ggtgataaag tgattgataa gtcatcatct
     8041 atatcactaa cagtcgttgg atttgtccat aatagtatgt atggtcacgg acctgtggca
     8101 tttattgata aagacattta taccgaaatt aataaaaaga ttaatcctca ataccagttc
     8161 ttacctcagg cattagttat gaaaaatgat aaaagtatat ctcatttacc aactcaatta
     8221 gaagctgtta gtaaaaaaga tgtcattcaa catattcctg gttatagtgc tgaacaatcc
     8281 actttgaata tgattttatg ggtccttgtc gtagcttcag ctggtatttt aggtgtcttt
     8341 ttctacatca tcacacttca aaaacgtcat gaatttagcg tcatgaaagc tattgggaca
     8401 aaaatgtcag agattgccct atttcaattg agtcaagtca tcatattggc actgtttggt
     8461 atcattgttg gagatcgatt ggcgattgct ctctcttatg tattaccagc tcaaatgcca
     8521 tttgttatta attggcaaaa tattattcta gtatcgtttg tatttttagt cattgcaatg
     8581 attagtagtg ctttgtcaat tgtaaaagtt gctaaaatcg acccagtcga agttattaat
     8641 gggggaggag aataatgtca gcaatcttag aattaaaaca catctcaaaa cactatccag
     8701 atggagatga gcttttatca atattagata atttagattt atcagtttct gcaggtgagt
     8761 ttgtagctat actgggaccc tcaggttcag gtaaaagcac acttttatcc attgcagggt
     8821 tacttcttgg tgcagatcaa ggtagtttgt atgtcaatca tgagaacgtt acggatttgt
     8881 ctcaaagaca aagaacacaa ttgcgccgtg aagcactagg ttttatattt caaagtcatc
     8941 aactgcttcc ttatttaaca atccaagaac agttgcaaca agaggctcga tttgcaaaac
     9001 actatgataa gaaaacaagc ttggaagaaa ttaataaatt attatcagat ttaggaatag
     9061 agcagtgtgc acataaatac cctaaccaat tatcgggagg acaaaagcaa cgcgcagcaa
     9121 ttgctagagc atttattaat catcctaagg ttatcttggc agatgagcca acagctagcc
     9181 ttgatgaaga acgtgggcgt caagttacag aacttattcg tcaggaagtt aaatcacata
     9241 atactgcagc tattatggtg acacacgatg agcgcgttct agatttagta gataccgttt
     9301 atcgtttaaa ggacgggaaa ttagttaaag aaaacttcta acttgtgtta caataagcta
     9361 gaggtgattt aatgattaaa atattagttg tagaggataa cattgttcaa caaaaaatca
     9421 ttaccacaaa attaacacaa gagggctacc aatttatcac tgcttcaaat ggacaagaag
     9481 ctctaaattg tctagataca gaagaagtac aattgattat aactgatatc atgatgccca
     9541 tgatggatgg ttatcaatta attcaagaac tcaggtcagc tgcttacaat gtcccaatca
     9601 ttgttatgac agcaaaatca caaatggaag atatgacaaa agggtttggc cttggtgcag
     9661 atgattatat ggttaagcct gtgcagttac aagaattagc tttacgtatt aaggcactct
     9721 tgaggagagc taatattgta gctcaacatc agctaattat tgggaatact tgtcttaatg
     9781 aagatgaatt aagcttgaaa tattttgagc aagaaattgt atttcctcag aaagaattta
     9841 gggttctttt ccacttactt agctatccta ataggatttt cacacgttta gaactattgg
     9901 atagtatttg gggaatggat actgatttag atgagcgggt ggtagatgct tgcatcaata
     9961 aaattagacg aaaagtagaa cacttaccag attttaagat tgaaacggtg agaggggtag
    10021 ggtatcgtgc aaaaaatgac taagctaagg cgatttcggt ttccgttaag attttatttc
    10081 acattaatgt ttgtgttaac gatgcttttc tcagtcctag cttctctttt actagttgca
    10141 gcaatagttt ttactttttt tcaaggagtc ttaactactc atgtattaca agttagcgct
    10201 ctcgctgttg tttttttaag ccttgtgata gctagtatta gtatgtggta cggttcttat
    10261 catttaacaa aaccaatttt agatattagc catattgtgt ccaatgttgc aggcggtgac
    10321 tttgaagggc atatttatcg aaacagtaat cgtcgtaaat cctatgagta ttataatgaa
    10381 ttggatgagc tatctgaaag tattaatcag atgattgttt ctttatctca tatggatcat
    10441 atgcgaaaag actttatcac taatgtttct catgagctaa agactccgat tgctgcagta
    10501 gctaatattg tagaactatt acaagatcca gaattagatg aagaaacaca aagtgagtta
    10561 ttaggtcttg ttaaaacaga gagcctaagg ttaacaagat tatgtgatac gatgttgcaa
    10621 atgtcgcgcg tggataatca ggagactatt ggggagctat cctctgttcg tgtagatgaa
    10681 caaattcgtc aagcaatgat tagtttaaca gaacgttggc aagctaaaag gataaatttt
    10741 caactggatt caaaaccata tactgtttac tcaaactctg acttgctgat gcaagtttgg
    10801 ataaaccttt tagataatgc aataaaatat tccgaggata ttgtagactt aagtgtacga
    10861 atggaagaaa ctaataatca ttatctgaga gttattattt ctgataaagg taggggaatt
    10921 tctcaatatg atgttcagca tatatttgat aaattttacc aagcggatca gtcgcataat
    10981 caacaaggaa atggattagg actagcaata gttaaacgta taattgttct ttgtaaggga
    11041 catattagtg tctccagtca gttggaaatt ggaacagaat tttgtgtaga actaccttta
    11101 tcataattat ttaaaacaat aactgccaat tggcagttat tgttttattt gtttagaaat
    11161 aaatatacta aaaatagaca aaaactataa aaatatttga taataaatat agcaagtgta
    11221 taatattaca aaatatagga atttggaagg ggatttttta tgggaaaaga aaaattaatt
    11281 ttagcttatt ctggtggttt agatacctct gttgctatag cttggttaaa aaaagattat
    11341 gacgttattg ctgtctgtat ggatgttggc gaaggtaaag atcttgattt tattcacgat
    11401 aaagctttaa caataggtgc catagaatct tatattttag atgttaaaga tgagttcgct
    11461 gagcattttg ttttaccagc tttacaggca catgcgatgt atgaacaaaa atacccgctt
    11521 gtttcagcgc ttagtcgtcc aattattgcc caaaaattag ttgaaatggc acatcaaaca
    11581 ggtgcgacca caattgcaca tggatgtaca ggtaaaggaa atgaccaagt gcgttttgaa
    11641 gttgcaattg cagcactaga tcctgaatta aaagtaattg cccctgttcg tgagtggcag
    11701 tggcatcgtg aagaggaaat tacttttgca aaagctaacg gcgtacctat tccagcagat
    11761 ttagataacc cttattctat agaccaaaat ttatggggac gtgccaatga atgtggtgtg
    11821 cttgagaatc cttggaatca agcgccagaa gaagcttttg gtattacaaa atcaccagaa
    11881 gaagctcctg attgtgcaga atatattgat attacgttcc aaaatgggaa gcctatagct
    11941 ataaacaatc aagaaatgac actagcagat ttgattttat cgctaaatga aattgcagga
    12001 aaacatggta ttggacgcat tgatcatgtt gaaaatcgtt tagtcggtat caaatcacgt
    12061 gaaatttatg aatgtcctgc ggcaatggta ctgttagctg ctcataaaga aattgaagat
    12121 ttaacattag tccgtgaagt gtctcatttt aaacctatcc ttgaaaatga actatcaaat
    12181 cttatttata atgctttatg gtttagtcca gctacaaaag ctatcattgc ctatgtcaaa
    12241 gaaacacaaa aagtggttaa tggtactaca aaagttaagt tatataaagg ttctgccaaa
    12301 gtcgttgctc gccactcgtc taattcatta tatgatgaga acttagcaac ttatacagct
    12361 gctgataact ttgatcaaga tgcagcagtt ggttttatca aactctgggg acttccaacc
    12421 caagttaatg cacaagtaaa caaaggataa ataaaggaag ataagaggat gacaaaaaat
    12481 cataagttat ggggtggtcg ttttgaaagt agtcttgaaa aatgggttga agagttcggt
    12541 gcatcaatca gtttcgacca aaagttagct ccctatgata tgaaggcttc tatggcccat
    12601 gtcacaatgt taggcaaaac ggatattatt agtcaagaag aagcaggctt gataaaagat
    12661 ggtttaaaaa tactgcaaga taaatacaga gcaggtcaat tgacttttag catatcaaat
    12721 gaagacattc atatgaacat agagagctta ttaaccgctg aaattggaga agtagcaggt
    12781 aagcttcata ctgcacgttc acgaaatgat caagtggcta cagatatgca cctttatttg
    12841 aaagataaat tgcaagaaat gatgaagaaa ttgcttcatt tacgaacaac cttagttaat
    12901 ttagcagaaa atcatatcta tactgttatg ccaggatata cacatttaca acatgctcag
    12961 ccgatttcat ttggacatca tctaatggct tactataata tgtttacgcg tgatacagag
    13021 cgattagagt ttaatatgaa acacaccaat ctatcaccat taggagcagc agctctagca
    13081 gggacaacat ttcctattga ccgccatatg acaactcgtt tattagattt tgaaaaacca
    13141 tatagtaatt cgcttgatgc tgtctcagat cgtgacttta tcatagaatt tttatccaat
    13201 gctagcattt taatgatgca tttatcacgt ttttgtgaag aaatcattaa ttggtgcagt
    13261 tatgaatatc aattcatcac attatcagat actttctcta caggatcatc tattatgcct
    13321 caaaagaaaa atcctgatat ggcagaatta attcgaggga agacaggccg tgtttatgga
    13381 aatttattta gtttactaac tgttatgaaa tcattgccgt tagcttataa caaagattta
    13441 caagaagata aagaagggat gtttgatagc gtagaaacag tcagcatagc tattgaaatc
    13501 atggctaata tgcttgagaa aatgactgtg aatgagcaca taatgatgac ctcaacggag
    13561 actgattttt caaatgcaac agaattagca gattatttag caagtaaggg ggtacctttc
    13621 agaaaagcac atgaaattgt aggtaaattg gttttagaat gcagtaaaaa tggttcttat
    13681 ttacaggata tacctttaaa atattatcaa gaaatttcag agttgataga aaacgatatc
    13741 tatgagatat taactgcaaa gacagcagtt aaacgaagaa attcattagg aggaacaggg
    13801 tttgaccagg ttaaagaaca gatcctgttg gcacgtaaag aattgaaagc ggaatgactg
    13861 ctttctgaaa aaacctcgtt taactctgtt aagtttctca cgtattttca gtaaaaaact
    13921 agcatttttt atagaattgt gctagaatta acatgtaagt gggcaacgcc caaaaaataa
    13981 ataggaggcc tgaataatgg caatcgtttc agcagaaaaa ttcgtccaag cagctcgtga
    14041 taacggctat gcagtaggtg gatttaacac aaacaacctt gagtggactc aagctatctt
    14101 gcgcgcagca gaagctaaaa aagctccagt acttatccaa acttcaatgg gtgcggctaa
    14161 atacatgggt ggttacaaac tttgtaaaca acttattgaa actttggttg aatcaatggg
    14221 tattactgta ccagttgcta tccaccttga ccacggtcat tacgatgatg cccttgagtg
    14281 tattgaagta ggttacactt caattatgtt tgatggttca caccttccag ttgaagaaaa
    14341 ccttgaaaaa gcacgcgaag ttgttgctaa agcacatgct aaaggtattt cagtagaagc
    14401 tgaagttggt actatcggtg gtgaagaaga tggtattgtc ggtaaaggtg aattagcacc
    14461 aatcgaagac gctaaagcta tggttgaaac tggtattgac ttcttggctg caggtatcgg
    14521 taacattcat ggaccatacc cagcaaactg ggaaggactt gaccttgatc acttgaagaa
    14581 attgactgaa gctgttcctg gtttcccaat cgtgttacac ggtggttcag gtattcctga
    14641 tgatcaaatc caagaagcta tcaaacttgg tgttgctaaa gttaacgtta acacagaatg
    14701 tcaattagct ttctgtcaag caactcgtga ctacgctgtt gaattcaacg ctaacgaagc
    14761 tgaatacatg aagaaaaaac tctttgaccc acgtaaattc ttgaaacctg gtttcgatgc
    14821 aattacagaa gcagttgaag aacgtattga cgtgtttggt tcagcaaaca aagcataatt
    14881 aacataaaac agataatagt taaaagctca cttatggtga gctttttttg gattatatga
    14941 ctttttatta aatagatact ctttaaagat tgatatgctt aagataattt cgaataaatg
    15001 ccgctgaatc tgtgagttgt tgctcttctt ttgggcttag tgaaagagaa attctttcta
    15061 caattcctac tctaccaata atagctggat atccaaggta aaccccttcg gactcataga
    15121 aggtagatac aggtagtaac tcatgggaat catttaacac agctaaaacg atgcggacag
    15181 ctgcggtagc gataccataa ctagtatatt ttttaccaaa aaagacagtg tgcccgcctt
    15241 ttaaagaagc ttcttttatc tgttctaatt cttctacttt taatagtgat atagctggtt
    15301 gtccttttac agttacctga ctccaagcga caaattggga gttaccatgc tctcctagat
    15361 tataaccacc gatactcctt ggatcaactt gtaggcgttg accaagcact cttttcatcc
    15421 gagcagtatc caataaggtt cctgttccga taacgcgtga tttaggaaat tgagtatatt
    15481 cttgataaag ctgggtgatg gcatcaacag gattagtaac gacaataagg atccctctaa
    15541 aaccagatga ttttaacttt tggctaacgt cgtagacttc tttactggta aatggtagtt
    15601 cagcaaagcg attagcttct ttgttattct gtagtgaaat atcacctaag gctgaaatca
    15661 caacatcagc atctgacaac gcgtcatagt cgtttaatac tatattagcg tgataagtag
    15721 catttgccag agcatcttga aaatcaatca tatcagcatt aaccttatca acattagtat
    15781 caataaagac ataatcatca ccaatacctt gtgcaataag agcgtgtgca acagttgatc
    15841 caacgtgccc catgcctata attccaattt tacgtgtcat atgaaacctc ctttgattat
    15901 tgttgtctat tataatattt tgaaagaaaa tgtaaactaa aaaatgaatt agaaggaagt
    15961 gtgtcttatt ataaaaaata tgtaagcgat ttagataaaa caaatgaaaa agtaattttg
    16021 aaaagaactt gctagaagaa gtaagttgtg atagaatagt ctagtatgtg gtgctagcac
    16081 atccgtaaat attgatcaat ataggaggaa ctcgtaaaat ggctaaagta tgttatttca
    16141 caggacgtaa aacagtttct ggtaacaacc gttcacacgc gatgaaccaa acaaaacgta
    16201 ctgttaaacc aaaccttcaa aaagttactg ttcttatcga cggtaaacca aaaaaagttt
    16261 gggtttcagc tcgtgcgctt aaatctggta aagtagaacg cgtttaataa actgagaaag
    16321 ccttagggct ttttttagtg tttctaaggc tgatttgata gttagatacc tctatttttt
    16381 tacaataata gtgagatatg atagaataga gtgataaaaa ctttttgagg tagtagatat
    16441 gactgtaaaa atcaatacaa gagatggtca aattgaactc tctgatgacg ttattgcaac
    16501 tgtcgttgga ggttcagcaa cggaaatttt tggcgttgtt ggtatggcaa gtaaaagtgc
    16561 tatcaaagat aactttcaag cacttttgcg taaggaaaac tacgcaaaag gtgttgttgt
    16621 gaggtctagt gaagcgggca tcgcagtaga tgtttatact gtcatgagtt acggcgtcaa
    16681 aattagtgaa gtttcaaaaa atattcaaga gcgagtgaaa ttcaatctcg aaaatcagct
    16741 tggtttatct gctgatatgg tgaatgttta tgtgcaaagt attaaggttg taggagaaga
    16801 ttagtgtcaa agattacgac aagtttattc caagaaatgg tacaagctgc cagtacacgt
    16861 ctgagtaaac aggcagagta tgtcaactca ttaaatgtat tcccagttcc agatggtgat
    16921 actggtacga atatggggat gactatggaa aacggagcca aggaagtttc ggacaaacca
    16981 gcgacgactg ttggagaagt tggtcaaatt ctttctaaag gtgtattgat gggagcacgt
    17041 ggaaattcag gtgtaattac atcacaatta ttccgtggtt ttggtcaaag tatcaaggat
    17101 aaagaagaat taacgggtca agatttggca cacgctttcc agaatggtgt tgaagtagct
    17161 tataaagccg ttatgaaacc agttgaagga actatcttga cagtatcacg cggtgcagct
    17221 accgctgctc taaaaaaagc tgaagaaact gatgatgcag ttgaagttat gcgcgcaact
    17281 ctcaaaggtg ctaagcgtgc tcttgctaaa acaccggata tgctacctgt tcttaaagag
    17341 gttggtgttg ttgattctgg tggtcaagga ctcgtcttta tttacgaagg tttcctctca
    17401 gcactgactg gggaatatat cgcatcagaa gattttaaag caactcccgc aacaatgact
    17461 gaaatggtta atgcagagca ccataaagct gtagtaggcc acgtagccac agaagatatt
    17521 aaatatggct attgtacaga aatcatggtt ggtttaaagc aaggaccaac ttacgttaag
    17581 gaatttaact acgaagaatt tcaaggttat cttagcaatc taggggattc tttactagtt
    17641 gttaatgatg atgaaattgt caaggttcat gttcatacag aagatccagg tcttgttatg
    17701 caagaaggtt tgaaatatgg atcgcttgtg aaggttaaag ttgaaaacat gcgtaaccaa
    17761 catgatgccc aaatgcaaaa ggttgaagtt gaagaaactg tcaaagaaac taaagaatat
    17821 ggtattattg ctgttgtagc tggtgacggt ctagctgaga tttttaaatc acaaggtgtt
    17881 gattatatca tctctggcgg ccagactatg aacccatcta cagaagatat tgtaaaagcg
    17941 attgaaaaag taaacgctag aaatgttatt attttaccaa acaataagaa catcttcatg
    18001 gctgcacagt cagctgcaga tgtcgttgat attccagcag cagtagtaga gacacgtaca
    18061 gttccacaag gatttactag cttacttgct tttgatcctg ctaagtctct tgaaactaat
    18121 gtagctgata tgacaaatag cctctcagat gtcattagtg gtagtgtgac tttagctgtt
    18181 cgtgatacaa cgatagatgg ccttgaaatt catgaaaacg acatccttgg gatggttgat
    18241 ggtaaaattc tagtatctac accagatatg gaaaaagcac ttaaagacac ttttgataaa
    18301 atgattgatg aagatagtga aatcgtgaca atctatgttg gtgaagatgg taaacaagca
    18361 ttagccgaga ccttgtcgga atatcttgag gagacttatg aagatgttga agttgagatc
    18421 caccaaggag atcaacctgt atatccatac ctcatgagcg ttgaataatt gataaaataa
    18481 aaaattagga gagatacttc taatttttta ttttatcaga gtttctattt tagtatgata
    18541 aagcatcagt attttgaaaa ttttgacaaa ttgtgctaga attgttttaa aacattcata
    18601 caaaggagat ttatgatgtc attaattatt cttacggtta ttttggtttt agtgattgtt
    18661 ctgttgataa cttctttata cgttgttaaa caacagacgg tagccatcat tgaacgcttt
    18721 ggtaaatatc aaaagactgc tactagcggt attcatattc gtgtaccatt gggtatcgat
    18781 aagattgcag cacgagtgca gttacgtctt ttacagagtg aaattatagt tgagacaaaa
    18841 acaaaagata atgtttttgt cactttaaat attgctacac aatatcgtgt caatgaaaat
    18901 aacgttacag acgcttatta caaattaata aagccagaag ctcagattaa atcttatatc
    18961 gaagatgctt tgcgttcatc agttcctaaa cttacattag atgagctatt cgaaaagaaa
    19021 gatgaaatag ccttggaagt tcaacatcaa gtggcagaag aaatgtcaac atacggctac
    19081 atcattgtta agactttaat tactaaggtt gagccagatg cagaagtaaa acaatccatg
    19141 aacgaaatca atgctgcaca acgtaaacgt gtagcagctc aagaattagc gaatgctgat
    19201 aagatcaaaa ttgtaactgc tgcagaagca gaagcagaaa aagatcgtct acatggtgtg
    19261 ggtattgcgc aacaacgtaa agcaattgta gatgggttag ctgattcgat tcaagaattg
    19321 aaagatgcta acgtgacatt gacagaagaa caaatcatgt ctatcttgtt aacaaatcaa
    19381 tatttagata ctttaaatac atttgctatt aatggtaatc aaacgatttt cttaccaaat
    19441 aacccagaag gtgttgaaga tattcgtaca caagttttat ctgctcttaa aacaagataa
    19501 tgaacttttt atagaagcaa aataaccaga gcatttgctc tggttatttt gtgtctgact
    19561 tatttattat aattggagag acggaaaaaa gttatttttt accgtaacgt ttgtactcat
    19621 gaatgatttt cagagcacgt tttctagttt taatgttagg acttttaagt ctgcgatagg
    19681 ctgacgatag gtctgttttt tcagccatga ttacctccta tagacaattt attaacgtta
    19741 acagacacca gtataccaca aaaaaattaa ctggtaaagt actttgttga taaatttaaa
    19801 aataaatagc aaagtaatta aatatcaatc aaaagtaata agttcaaaga tatattaaac
    19861 ctttattaat gaggtttaga tttgacaagt gatgttaagt gaaagatgaa tgaggaaaat
    19921 acccaacgag aaagtagcta atggctatca ttgtgcatat aaaaaaacga tcgatgagtg
    19981 atcgtttttc agcaaactaa ataattattt ttaatgtaat tgaatgaaaa tttatacttg
    20041 tgaaagtgta tcggttaagt taataaggtc agtttttatt tatcgtaagc acttaggaag
    20101 tgttttggag cgtatccacg agagtagata aacccggcat cgcgtgaagg atagcttgat
    20161 tcagaaggcg catacccacg agagtagata aatccggcat cacgtgaagg ataagacact
    20221 gtgtctgcct tcgcaagact tgctgaagta gctaataata gagtgacagc tgaagctgct
    20281 gttaaaacac tgattttaag tttcattata aaatcctttc aaagataata aactattctt
    20341 gcacagtggt tagttcattt tgtgcatact atattatatc atcaaaagct cattaaaaca
    20401 acatgttttt aattttaaac aaatatataa ttaattaaat atcgattaaa attaaaaaat
    20461 gagatttagt aacttattta atagtataca atccaataac gactgtctaa tttgatctat
    20521 agttgaggct agtcgtagat ttcatcaaag atgtggtaat aaaaaatccc ctccagttcc
    20581 attgtaatga tgacaatgac aaaacctgta ggggtataaa tagtctatat ttaaatgtgg
    20641 ataaatttta ttttaagaag cgttgtagaa attccttagt tctttcttgt gttggatttt
    20701 cgaaaagttg cttaggagta ccctgctctg caatgattcc tttgtccatg aaaataacgc
    20761 ggtctgatac ttctttagca aattccatct cgtgtgtcac aattatcatt gttaatccag
    20821 atttagctag atcttgcatt gttttaagga cctcaccaac catttctgga tccaaggctg
    20881 aagttggttc atcaaaaagt atagcttctg ggttaacaga aagtgctcgg gcgattgcga
    20941 cacgttgctt ttgcccacct gataattgtt ttggtttagc tttccaatat tgctcagtca
    21001 tgccaacagc atttaagttt tctttagcaa tcttttcagc ttcttggcgt tcacgtttta
    21061 gaacagtagt ctgtgcaaca atagcgttct ctaagatgtt taaattctca aagaggttaa
    21121 aagattgaaa gaccatccca agtttttcac gataattatt taaatcatag cctttttcaa
    21181 gaacattgtg accgtgatat aagatttctc ctccacttgg ttcttctaag aggttaatag
    21241 aacgtaggaa agttgatttt cctgatccag aagaaccaat gatagaaata acttcgcctt
    21301 tgttgaccga aagcgaaatg tcttttaaca cttcattgct tccgtaagat tttttcaaat
    21361 gtttaatttc taaaatagct tgtgtcatac caacccccta gacttgtaat tgattcgcac
    21421 cggttgtgta attatctgaa tcaaaacgtt tttcaatgta acgtaagata cgtgttacgg
    21481 tgaaggttaa gatgaagtaa ataatcgcaa taatggtgaa tgtttggaaa tattggtagg
    21541 tttgtgttgc aacagtatta ccagagaagt agagttcgac aacagagata acgttcagta
    21601 cagaggtatc tttgatattg ataacaaact cattaccagt agctgggagt atgttacgta
    21661 caacttgtgg tagaacaatt tttcgcatgg tttgtccatg ggtgaaacca agtgcagttg
    21721 cggcttcaaa ttgtccttta tcaacagaga aaataccacc acgtacaatt tcgctcatat
    21781 aagcaccagt attgattgaa acaataaata tagctgctag tgtacggtcc agcgaaactc
    21841 cgaaagcttg tgctgttccg tagtagataa ccattgattg tacaatcata ggtgtcccgc
    21901 ggaagacctc aatatagatg ttaagtaacc aacctaataa tttttgtaag gcagccttta
    21961 acttattgtc agattttggt gctgtacgga aaacaccgat gagaaggccg ataaataatc
    22021 cgacgatagt tcctaccata gaaattagga gggttgtagc tgttcctctg aggaattgag
    22081 aaccattgtt ttttaaaata gttgccatct gttcaaagaa gtttggtttt ccattttttt
    22141 cttttttaac ggatggttgt tctttaatca tcttatccat caaggcgatt tgtttatctc
    22201 tagaaatgct ttcgagaact tgattgactt gattaatacg attgtcacct ttacgcatcc
    22261 caacggaaat atttgtatcg gcatcagatg ttttaaaccc ttggtgcagt tcaatcattt
    22321 ttaatttagg attagcagtt tgagcggaag ttgcatctgg acgttctgaa acataggcgt
    22381 ctacaacacc agattctaca gcttgtctta attgattaaa gtcccccatg gcaacttctt
    22441 ttttgacacc attgatttga tcaatgaggt tgtataagta gactccttgt tgggcagtta
    22501 ctttagcatt tttgaaatca ctaatatttt tagcattagt atattttcct tcagcgttaa
    22561 caactagagt aggttctgaa atgtagtaag gctttgaaaa attaatttct tttttacgtt
    22621 cttcggtagg actcattcct gcaataatca tatcgagttt tccagaagtt agtgctggaa
    22681 caaggccttc ccattttgtt ttgacaacaa caactttttt atttaacttt ttagctaact
    22741 ttttagcgac ttgtacatca taaccattag catattgatc agttccttca ataggaactg
    22801 caccatttgt gttatcattt tgtgtccagt tgaaaggcgc ataggctgcc tccataccga
    22861 cacgtagata ttcatctgct tgtatacttg tcatacctcc aaaggtgata agcaaagcag
    22921 tgaataaact tagtaatatt tttttcattc aacagcctcc gataagttac ttcctcctat
    22981 tttaccgaaa aacaaggtcg atttcaatat cattatctta ttgaaagcga ttaattgtat
    23041 ttttatgtta aaatggtata caaacaaagg agggaggatg ataatgaaga aatgtttttt
    23101 ggctatttgt ttagctctta gtttttttat ggtttcagtt caagcagatg aggtggacta
    23161 taacattcct cattatgagg gtaatctaac tattcacaat gataatagtg ctgattttac
    23221 agagaaggtt acttaccaat ttgattcgtc ctataatgga cagtatgtca cgttaggtac
    23281 ggcgggtaag ttacctgaca attttgatat taataataag ccacaggttg aagtttcaat
    23341 taatggtaaa gtaaggaaag ttagttacca gatagaagat ttggaggatg gctaccgttt
    23401 gaaagtgttt aatggtggtg aagcaggtga tactgttaaa gtcaatgttc agtggaaact
    23461 aaaaaatgtt ctatttatgc ataaggatgt tggtgaactt aactggattc ctattagcga
    23521 ctgggataaa acgttagaga aagtagattt ttggatatca actgacaaaa aggttgctct
    23581 ttctcgtctt tgggggcact tgggttatct taaaactcct cctaaaataa gacaaaataa
    23641 taatcgttac catttgacag cttttaatgt aaacaaacga ttagaatttc atggttattg
    23701 ggatagatct tattttaatc tacctacaaa cagtaaaaat aattacaaga aaaaaattga
    23761 acatcaagag aagataatag agcgtcatgg ttttatccta agtttcttgt taaggatatt
    23821 attaccttca ttctttatta ttgtgacact attcatctca attagggtgt tcctgtttag
    23881 aaaaaaagtt aataaatacg ggcaattccc taaggatcat catttatatg aagcacctga
    23941 ggacctttca ccattagagt taactcaaag catttatagt atgagcttta aaaattttca
    24001 agatgaggag aagaaaactc accttatcag tcaagaacaa ctcatacagt caattctatt
    24061 agacttgatt gatagaaaag tattgaatta tgatgataac ttgttatctc tagctaactt
    24121 agatagagct tctgatgcag aaatagattt tatagagttt gcttttgcgg attctacgag
    24181 tttgaagcca gatcaactct tttctaatta ccaatttagt tataaagaaa cactacgtga
    24241 actgaaaaag cagcacaagg cttcagatct gcaaaatcaa atgagacgcc gaggaagtaa
    24301 tgccttatca agaattacgc gtctcacaag gttgatttct aaagacaata taaactctct
    24361 tagaagaaag ggaatttcat ccccttatcg taaaatgtct tcagaagagt ctaaagaatt
    24421 atctaggtta aaaagattca gttacctatc acctcttatt tcttttgttg ttataattta
    24481 tacgcttttt ttaaattatt ttacctattt ctgtatctat ctcttattgt ttggtgttat
    24541 cctgttgttg aataaaatca tttttatgat gacaagaaaa ataagtaacg gttatattgt
    24601 aactgaagat ggagcaagtc gtgtctacca atggactagt tttaggaaca tgctaaggga
    24661 tatcaaatcg tttgatcgtt cagagttaga aagtatcgta ttatggaatc gaatattggt
    24721 ttacgctact ttattcggct acgctgaccg tgttgagaaa gtactcagag tgaaccaaat
    24781 agatattcca gaaagatttg caaacattga tagtcatcga tttgcgattt cagtcaatca
    24841 atctagtaat catttttcaa cgataactga agatgttagt cacgcttcta attttagtgt
    24901 taattcaggc ggttcttcag gtggtttctc aggcggcgga ggcggcggag gtggcggtgc
    24961 cttctaattt tccaaaagta tggtataata aagaaaaatt tatggagtta ttatgttaat
    25021 aattgaatta ctcaaagccc tatttttagg agtcgttgag ggagtgacag aatggttacc
    25081 tgtttctagt actggccatt taatcttggt tcaagagttt atgaagttga accaaagcaa
    25141 atcctttgtt gaaatgttta atattgttat acaacttggg gcaattatgg cagttattgt
    25201 catttatttt aaacgcctta acccatttca gccaggtaaa tctgctagag aaattcgttt
    25261 aacttggcaa ttatggttaa aagtagtgat agcttgtatt ccatctattt taattgcttt
    25321 accatttgat aattggtttg aagctcattt taatttcatg attccgattg caatagccct
    25381 aatcttttat ggttttgtct tcatatgggt tgaaaaacgt aatgcacacc tcaaaccaca
    25441 ggtaaccgaa ttggcaagta tgtcttacaa gacagctttc ttgattggat gtttccaggt
    25501 tctcagtatt gttccgggaa ccagtcgttc tggagctact attttaggag caattattat
    25561 tggaactagt cgttcggtcg ctgctgactt tactttcttc cttgccatcc caactatgtt
    25621 tggttatagt ggacttaagg cggttaaata ttttttagat ggtaacgtct tgagtttaga
    25681 ccaatcttta atacttttag tagcaagtct gacagctttc gtagttagtt tatatgttat
    25741 tcgtttcttg acagactatg tcaaacgaca cgatttcacc atctttggta agtatcgtat
    25801 agtcttagga agtttactca tcctctactg gttagttgtt catttattct aaaatataac
    25861 ataaaaagct gagtattcct cagcttttta gcgtgttagg ctgtgaaaaa ttgctaaaaa
    25921 atagcttttt cggtataata agaaaactga atactcgtat gaggtgatga tatggaaatg
    25981 aaacaaatca gtgaaacaac actgaaaatt acaattagta tggaagattt agaagatcgt
    26041 ggtatggagc tgaaagattt cctaatccct caggagaaga ctgaggaatt tttctattct
    26101 gtcatggatg aattagactt gccagaaaac tttaaaaata gtggtatgtt aagttttcga
    26161 gtaacaccta aaaaagatcg cattgatgtt tttgttacaa agtctgaatt aagtaaagat
    26221 ttaaatttag aagaattagc agatttgggt gacatttcaa aaatgtctcc agaagacttt
    26281 tttaaaacct tggaacaatc gatgttggaa aaaggggata cggatgccca tgccaaatta
    26341 gcagaaattg aaaatatgat ggataaagca actcaagaag tagttgagga aaatgtttct
    26401 gaagaacaac ctgaaaagga agtagaaacg attggatatg ttcactatgt ctttgatttt
    26461 gataatattg aagctgtagt tcgattttca caaacgattg attttccaat agaagcttct
    26521 gaactctata aaaatggtaa aggctatcat atgacaattt tgctagactt ggaaaaccaa
    26581 ccaagctact ttgctaattt gatgtatgct cgaatgcttg aacatgctaa tgtgggcaca
    26641 aaaacacgag cttacttgaa agagcatagt attcaattga ttcatgatga tgctatttca
    26701 aaattacaaa tgattgagat gggataaaat gatacctttt acaattgagt atatttttgt
    26761 attgattgga gcatttttat tatcaatcat tctaactcct attattcgag ttatatcttt
    26821 gaaggtagga gcggttgata aaccgaacgc tcgaagaatc aacaaagtac caatgccaag
    26881 tagtggtggt ttagcaatat ttctctcctt tgttgtaaca acattatttt tcatgccaat
    26941 ggcggccagt agacacttta tagaagtctc ttattttcac tacattttgc ctgtaattat
    27001 tggcggtctc gtagttacaa caacaggatt tatagatgac atctttgaat taagacctcg
    27061 ttataaaatg ttgggaatta ttattgcagc tattatcata tggaaattta cacattttag
    27121 atttgatagt tttaagattc caataggtgg tccactcttg gagtttggtc ctattttaac
    27181 tttctttttg acagttttat ggattatttc aatcactaat gctatcaatc tgattgatgg
    27241 tctagatggt ctagttagtg gtgtttcaat catcagtcta gcaacgatgg cagtggtttc
    27301 ttatttcttt ttacctaaaa tagatttttt tctcacgcta actattgtta ttttaatcgc
    27361 ctcaatagtt ggttttttcc cttataatta tcatccagcc attatctacc taggtgatgc
    27421 tggtgccttg tttataggat ttatgattgg tgtattatcg cttcaaggtt taaaaaactc
    27481 cacagctgtt gcagtgataa cacctgtcat tattttagga gttcctattt tggatacagc
    27541 tgttgctatt gtgaggcgta aattatcagg gaaaaagatt tctgaagctg ataagatgca
    27601 cctgcatcac cgattgctat ctatgggatt tacacataga ggagcagttt tagttgttta
    27661 tggtattgca attatctttt cgcttatcgc tttgctttta aatgtttcta gtcgtattgg
    27721 gggaattttt cttctattgg ctctactatt agtaatggaa atttttatag aaggtttaaa
    27781 tatttggggt gagaatcgga caccgctatt taatctctta aagttcattg gcaacagtga
    27841 ttacagacaa agtgttattg ctaaatatag cgataagcat caaaaataaa agcctgaaaa
    27901 ggctttttta gttttaatca tttagatagc agtcacacct catttttgct ataattattt
    27961 agaatataat atcgggtgag tgccctataa tatcttcttt aaaccataga gaaatatcac
    28021 atattctctg ttaaccaaga aaaggagtgt acaatgtctg tacttgaaat caaaaatctt
    28081 catgtttcta ttgaagataa agaaatttta aaaggtctta atttaacgct gaaaacagga
    28141 gaaattgctg ctatcatggg tccaaatggt actgggaagt cgacactttc agcagctatt
    28201 atgggaaatc ctaattatga agttacagca ggtgaaattc tttttgatgg tgaagatatt
    28261 ttagagctag aagtggatga acgtgctcgt ttgggtctat ttcttgcgat gcaataccct
    28321 tcagaggttc cagggattac taatgctgaa tttatccgag cagctatgaa tgcgggaaag
    28381 gctgatgatg ataaaatttc catccgccaa tttattacta agttagatga aaagatggaa
    28441 ctacttggca tgaaagaaga aatggcggaa cgttatctta acgaaggttt ttcaggtggt
    28501 gagaagaagc gcaatgaaat tttacaactc ctaatgttag agcctaagtt tgccctcttg
    28561 gatgagattg actctggcct ggatattgat gctcttaaag tggtatcaaa gggagtcaat
    28621 gaaatgcgtg gtgaaggttt tggtgctatg attattacac actatcaacg ccttttgaat
    28681 tacattacac cagataaagt acacgttatg atggacggga aggtagtatt gtcaggagga
    28741 ccagaattag cggttcgcct tgagaaagaa ggttatgctc aaatcgctga agagcttggt
    28801 ttagagtata aagaagaagt ctaatttgtc acttcaacta gaagagaaaa ggagttacaa
    28861 atgtctaaag aagctatatt aaatttttta caagccaaag gtgagcctac ttggttacaa
    28921 gaattacgac taaaggcttt tgaaaaaata gaagaactag aattaccagt aattgaacgt
    28981 gtaaaatttc atagatggaa tttaggtgat ggaactattt tagagaatga ctatacagct
    29041 aacattccag atttcacaga gttaggaaac aatcccaagt tggttcaaat tgggacccaa
    29101 actgtccttg aacaagttcc tatggaatta atagagaaag gcgttgtctt tacagatttc
    29161 tattcggctt tggaagaaat tccagaagtc atagagcgct actttgggaa agcacgtcct
    29221 tttgaagaag atagacttgc tgcctatcac acagcttact tcaacagtgg tgcagtgctt
    29281 tatattccag ataatgttga aattactcaa ccaatcgaag gtctttttta tcaagatagt
    29341 caatctaaag taccatttaa taagcatatc cttcttattg ttggaaaaaa tgcaaaagtt
    29401 agttacttag agcgatttga gtcaattggt gatgggacag aacgaacaag tgctaatatt
    29461 tctgtagaag taattgccca agcaggtagc caaattaaat ttgcctcaat tgatcgcctc
    29521 ggagaaaatg tgacaacgtt tataagtcgt cgtggtcgtc acagtagcga tgcaacaata
    29581 gattgggccc ttggggtgat gaatgagggc aatgttgttg ctgactttga ttctgattta
    29641 attggtgatg gaagccacgc taatttaaaa gtagttgctg cttcgtcagg gcgtcaagtt
    29701 caaggaattg atacgcgtgt tacaaactac ggctgtaatt cagtaggaca tattttacag
    29761 catggggtta ttttagagcg tgggactctt acttttaacg gtatcggaca tattataaaa
    29821 ggtgcaaagg gagcagacgc ccaacaagaa agccgagttc tcatgttatc agacaaagca
    29881 cgttcagatg ctaatcctat ccttttgatt gatgaaaatg atgttacagc tggtcacgca
    29941 gcctcaattg gtcaggttga cccagaggat ttatattatt tgatgagccg tggcttgaac
    30001 caaaaaacag cagagcaatt agttattcgt ggatttttag gaacagtcat tgctgaaata
    30061 ccagtaaaag aagttcgcga tgaaatgatt gccgttattg atacaaaact agaaaaacgt
    30121 taatatgaca ttactagata gttataaact aaagcaagat ttcccaattt taaatcaatt
    30181 agttaatgat gagccgctta tctatttaga taatgcagca acgacacaaa aaccgaatca
    30241 agttttagaa actttgcgag attactacca aaatgacaat gcaaatgtcc atcgtggtgt
    30301 tcatacttta gcagaacgag cgactgctca gtacgagact gctcgtgaaa aagcaaggca
    30361 gtttttaaat gctaaatcaa gtaaagaaat tttattcaca cgtggcacca caacaggatt
    30421 aaattgggta gctaagtttg ccgaaagtat tttagaacga ggtgatgagg tacttatttc
    30481 cattatggag catcattcta atattatccc atggcaacaa gcttgcgaga gaactggtgc
    30541 taaattggtt tatgcctatc taaaagatgg ttctttagat ttagaagatt tctataataa
    30601 attatcctct aaaactaaat ttgttagttt ggcacatatc tcaaatgtac ttggctgtat
    30661 tacaccagta aaagcaatag cagaacgtgt gcatcaagtt ggggcataca tggttgttga
    30721 tggagctcaa tcagcgccac atatggcgat tgatgttcaa gaccttgatt gtgatttctt
    30781 cgccttatct ggccataaga tgcttggtcc tactggtatt ggtgttcttt atgggaaaga
    30841 aagcatttta gataagatgc cacctgtcga atttggtggt gaaatgattg attttgtcta
    30901 tgagcatagt gcaacatgga aggaattgcc ctggaagttt gaagctggaa cgcctaatat
    30961 tgctggagcg atcgcttttg gagaagcttt agattattta acggatgttg gtatggatga
    31021 gattcatcaa tatgaacaaa gtttagtttc ttatgtgttg cctaaattac aggctatcga
    31081 tggtttaact atttacggac ctagtgatgc tgaatctcat gtaggcgtta ttgccttcaa
    31141 tttagaaggt ttacacccac atgatgtggc aactgcaatg gattacgaag gagtggctgt
    31201 tcgtgcaggt catcattgtg cacaacccct tattaatcat cttggtatcc attcggcggt
    31261 tcgagcaagt ttctatttct ataataccaa agaggattgc gacaaattgg tagacgctat
    31321 ccaaaaaact aaggagtttt tcaatggcac tctctaaatt agataacctc tacatggcgg
    31381 tggtagctga tcattcaaaa caccctcatc atcatggatt tttagaaggc gttgaacaag
    31441 tgcagttaaa caatcctaca tgtggagatg ttatttcctt atctgttaaa tttgatggga
    31501 atattatttc agatattgca tttgctggta atggttgtac aatttctacg gcttcatcaa
    31561 gcatgatgac agatgcagtt attgggaaaa caaaagaaga agcattgcag cttgctgatg
    31621 tattctccaa aatggttcaa ggcaatcaaa atccaaaaca agagaaactt ggggatgcag
    31681 agtttctagc tggggtatca aaatttccac aacgtatcaa atgtgctacg ctttcgtgga
    31741 atgctttaag aaaggctatt gagagagata atcaggcaga actctgacaa ccatagttga
    31801 aggaattaca tttaaacaac ttttactgac ataaggtagt ctagtagaag aatgtcttaa
    31861 atttaaatat taaagaaagg ataagtatgt ccgaaataaa tgaaaaagta gaaccacagc
    31921 caatagatct tggggaatat aaatttggat ttcatgacga tgtaaagcca atttattcta
    31981 cgggaaaagg tctaaatgag gctgttattc gtgagttatc tgcagctaag ggtgaacctg
    32041 agtggatgtt ggactttcgt ctaaaatcct tggaaacgtt taataaaatg ccgatgcaga
    32101 cctggggagc agatttatca gatattgatt ttgatgatat tatttattat caaaaagcat
    32161 ctgataaacc tgcgcgtgat tgggatgatg ttccagaaaa aatcaaagaa acttttgaaa
    32221 gaattgggat tccagaagct gaaagagcct atcttgcagg agcatcagca caatatgaat
    32281 cagaagtagt ttatcacaat atgaaagaag aatatgataa gctgggtatt gtttttacgg
    32341 atactgactc tgcacttaaa gagtacccag agctattcaa aaaatatttt gctaaacttg
    32401 tccctccaac agataataaa ttagctgctc tgaactctgc tgtatggtca ggtggaacat
    32461 ttatttatgt tcctaaaggt gttaaggtgg atattccact tcaaacttac ttccgtatta
    32521 ataatgaaaa tactggacaa tttgaacgta ctctcattat tgttgatgag ggagcaagtg
    32581 ttcactatgt tgaaggttgt accgccccaa cttattcttc aaatagttta catgcagcta
    32641 tagttgaaat ttttgcactt gatggagctt atatgcgcta tacgactatt caaaattggt
    32701 ccgataatgt ctataattta gtgacaaaac gtgctaccgc taaaaaagat gcaacagttg
    32761 agtggataga tggaaatcta ggagctaaaa caacaatgaa atacccatcg gtttaccttg
    32821 atggtgaagg agcacgtggc acgatgttgt ctattgcttt tgcaaacaaa ggacaacacc
    32881 aagatacggg tgcaaagatg attcataatg ccccccatac tagttcatcc attgtctcta
    32941 aatcaattgc taagggtggg ggaaaagttg attatcgagg tcaagtgaca tttaataaag
    33001 attccaaaaa atcagtgtca catatagaat gtgacaccat attgatggat gatatttcaa
    33061 aatcagatac cataccgttt aatgagattc ataattcaca ggttgcttta gagcatgaag
    33121 caaaggtgtc taagatttct gaagagcaac tgtactactt gatgagtcga ggtttatctg
    33181 aagctgaagc aacagaaatg attgttatgg ggtttgttga gccctttacg aaagaattac
    33241 caatggaata tgcggtagag ttaaatcgtt taatttccta tgaaatggaa ggttcagttg
    33301 gttaattgaa gatttcaaga gataataaaa agcccctatg ttattgatga aggggctttt
    33361 atattagcac agttattatc tagagcgctt tataagtaat actatcccag ctaaaagaca
    33421 aaggctagcg atggtgccca gaacaattaa aatagataca aaatgtgatt ggataaattg
    33481 atctgttttt gtccaccact gttcttttgt ttgaggctgt tcttgaatag atttagtttt
    33541 ggttttagta acttcttttt taggtttaac ggcttttaat acaggagttt tagaagaaat
    33601 atttttagcg tctttaaaac ctttttctac aagagcgttg acaaacggat gacgatagta
    33661 ctctccgtct tggtctgacc aatcgccaac tcctaaaacc acagttatca agcgagtatt
    33721 ctggcgttta gctgtaacta aggcattaaa agcagcgcta gggctagaac cagtttttaa
    33781 gccatctact ccttctaatc ccaatttagc gccgggggta gagtagttat aagttgtaaa
    33841 tgtttcttca taaggagttc cgaccatggc cttgacttca ggatattttg tatagttcag
    33901 tatatcaggg tattttttaa ggaaatgata ggttaaaatt gatagatcac gtgcagtcgt
    33961 aacgttagta gcattgttat cgtattcttt tggggagtaa agtccattaa aagcactcgc
    34021 taccgcccca ctggggttat aaaagtgagt ttttgtcata ccgagtttct tggcggtttc
    34081 attgattcgt ttaataaagg cgtcaggatt gttttgtgat aagtggttag caatcataat
    34141 agttgctaca ttagatgacg ggacagccgt catagtaatc agttcacgaa taggataagc
    34201 aaccccagca tgaatattgt tattactaat ttcataaatc ttacttatgg cttggtctgt
    34261 ttctgttgca gtcactgtgg tgttgaggtt tgtttttcct ttagctaagt cttcaaatag
    34321 taaatataaa gtaaacattt tagacatgct agcgggatca cgttctaaat cggcgttatc
    34381 ttcccacaaa atatgacctt tattgtcaat aacgatagac gctttaggtt tattaatatc
    34441 tttaacatca tatccggctt ctcgggtaat ttgcataata tctttatgtt cttcagcttg
    34501 cacagagttt acacaagtca gggataataa aatgcagagg aaagatacga ttaatttagg
    34561 cataggtaac tcctgaaaac tttttattaa tattataaca aaaaatcact aataaaaaag
    34621 acatttcatt agtgattttt aattttatat attctcctaa acctagctaa agaaactgct
    34681 gtgcagtaac tccttgtgtc aaagggagat tactattaaa gtttttcgtt aacgtagcgc
    34741 acaaaatgat tccaccacac tttgaggaag aaactgcgct caatactatc ttttgccact
    34801 aaatgaacgc taggttcctt tatgagatag ccttttccaa ccttattaag gtcaacgtaa
    34861 taagctttcc ctaggttctc tttttttgta ataggagcag taagctcttt tttaaagtta
    34921 atttttaact gattttgttt agtgatattt ttcttctgta ctactttcaa atcgttttta
    34981 gcaacagcga tgaccgattg ttcttttccg tctctcacac ttgcttcttt acctttatat
    35041 gcagctcctt tagctaatac aaggttaggt tcgtaggtgt ttgtgatata gttcaagaga
    35101 gagttagttg ctgtaaagcg agcatatttg tctttatcgg ccttatcagc atgcataata
    35161 acagtgagta gtctcattcc actttcagta gatgtagcaa taaaagattg gccagctaat
    35221 tccgttgtcc cagttttcaa acctgtaata cctggtctaa agacaggcat atctggtagc
    35281 atgtagttat aagaatgcat aatatcttta tcaaatttag caacggactt actagtaatc
    35341 ttaagaatgg aaggatattc gttgaccaaa tggtaggcag caatagcaat atcacgtgca
    35401 ctcattttat tttcgtcgtt ttgtgacgat tttggataaa tgtgattgcc taacatacta
    35461 ttatttaagc cagaagcatt gactaggtgg ctatcatgaa ttccccactt ttccaattga
    35521 gcagtcattt tatcaacaaa tttactttca gttcctgaaa tatgttcagc taaagcaatg
    35581 gctgcactgt tagcactaga aatcatggca gcgtccacga gttgtttaac agtatagcgc
    35641 cttttttcta aaggaacatt actagcatca gattcgcgtg ttagttgata agggtagtca
    35701 gatatattta ctttggtatt ccacttgagg ttaccgttat caatttcttt atagaccata
    35761 taaacggtca ttattttagt caaggaagca atagcagcgg gtttgttagc atctttttca
    35821 tacaagattt ttcctgaatc taaatcaact gctaaggcat gtttagcgga agcattaaaa
    35881 gattcagcag agatggttgg cataaaaaaa atgcaactaa gtaatagaat agaagtaata
    35941 atttttttca aaataacacc tttagaaatt ttttaaactt tgtccctcct attataccat
    36001 atcactatgt catactagga attattattt tcagataatt gaatgaagaa aacgtttgtt
    36061 ataaaaatgt aaaaaaatgt aaaaaaatga aagttaaaaa attatctgaa aattttgcgt
    36121 ttttgtcttt tcttaagaat tttttgtggt ataatgttag tcactagata aatgaattag
    36181 gagaacaaag ctatggcaaa acagaaaaat aactggcgcc gtgttggagt tggtgtcctt
    36241 acacttgctt cagttgcgac tcttgctgca tgtggaagta aatcagcttc ccaggattct
    36301 aatggagcga ttaattgggc tattccaaca gaaatcaata cactagattt atctaaagtt
    36361 acagacactt actcaaatct agctattggt aactctagta gtaatttcct tcgcttagat
    36421 aaagatggaa agacaagacc agacttggct actaaagttg atgtttcaaa agatggctta
    36481 acttatacag ctacattacg taaaggcttg aagtggtcag atggcagtaa acttactgca
    36541 aaggattttg tttattcatg gcaacgttta gttgatccta aaacagcttc acaatatgct
    36601 taccttgctg ttgaagggca tgtgcttaat gccgataaaa tcaacgaagg acaagagaaa
    36661 gacttgaata agctaggtgt taaggcagaa ggcgatgaca aagttgttat tactttatct
    36721 agtccgtctc cgcaattcat ctactacctt gcattcacta acttcatgcc acaaaaacaa
    36781 gaagttgttg aaaaatatgg aaaagattac gcaactactt caaaaaatac agtttactca
    36841 ggaccatata ctgttgaagg ttggaatggt tcgaatggta ctttcacgct gaagaaaaac
    36901 aaaaattatt gggacgctaa aaatgtaaaa acaaaagaag ttcgcatcca gactgttaaa
    36961 aaaccagata ccgccgttca aatgtataaa cgtggtgagt tagatgcagc taatatctca
    37021 aatacttctg ctatttatca agctaataaa aataataaag atgtcacaga tgttctagaa
    37081 gcgaccactg cctatatgga atataatact actggttctg tgaaagggct tgataatgtt
    37141 aagattcgtc gcgccttaaa cttagcaact aaccgtaaag gagttgttca agcagccgtt
    37201 gatacaggct caaaaccggc aattgctttt gcacctactg gtttagccaa aacaccagat
    37261 ggaactgatt tggcaaaata tgttgcccca ggttatgaat ataataaaac tgaagcagca
    37321 aaactcttta aagaaggttt ggctgaatca ggcttgacta agctaaaatt aacaattaca
    37381 gcagatgctg atgctcctgc tgccaaaaac tctgttgact atatcaagtc tacttgggaa
    37441 gctgctcttc caggacttac tgttgaagaa aaatttgtaa cctttaaaca acgcttagaa
    37501 gacagtagaa aacaaaactt tgacattgta gtttctcttt ggggtgggga ttatccagaa
    37561 ggatcaactt tctacggcct ctttaagtcg gattcacaaa ataacgatgg aaaatttgct
    37621 aacaaggact atgatgctgc ttataacaag gcaatttctg aagatgcgat gaaaccagca
    37681 gaatcagcga aggactataa agaagcagag aaaattttat ttgagcaagg tgcttataac
    37741 ccactctact tccgtagtgg taaaggttta caaaatccaa aattaaaagg tgttattcgt
    37801 aatactacag gtttgtcaat agactttaca catgcttata aaaataatta aattacttta
    37861 cactaaacct ttgcaagaat tggtaattgc cagttcttgc ttttggttaa gtaaagaaag
    37921 tgaaagagtt tgctattttt agcaattcta agaaaaggaa atgatatgat taaatatatt
    37981 ttaaagcgtg ttgctatttt gttagtaacg ctatgggttg tcataaccct atcgttcttt
    38041 ctgatgcaga tccttccagg aacaccctac aataatccaa aactcactga agaaatgatc
    38101 acattgctta ataaacagta tggtttagac aagcctgtat ggcaacaata tttgacttac
    38161 ctttggaatg tacttcatgg tgattttgga acgagttatc aatcagtgaa tcagccagta
    38221 tcacgtatga ttagcttgag attaggagtt tcagtacatc taggtgttca ggctctcgta
    38281 tttggtgttt taggcggtat tttggtagga gctatttcgg cacgtcataa gaatgataaa
    38341 gtcgatggta tcttaagtgt tattgcaacg ctagggattt ctatgccttc atttatcatt
    38401 ggtatcttat tattagacta ttttggattt aagtggaact tgttgccttt atcaggttgg
    38461 gggactttct ctcaaacaat tttaccttct ttagctctag gactccctac attagcatct
    38521 gtttcacgct tcttccgtag tgaaatgatt gaaactttaa attctgatta tgtgcaacta
    38581 gctcgttcaa aaggtatgac aattcgtcaa gtaactcgaa aacatgctta ccgtaattct
    38641 atgattccaa ttttaactct aattggccct ttggcggcgg gtctgttaac tggttctgca
    38701 ctaattgagc agattttctc gattccagga attgggcagc agtttgttac ttcgattcca
    38761 acaaaagact atcctgttat tatggggaca accattgttt atgcagtaat gttgatggtt
    38821 gctatcttga taacagacgt tgttattagt atcgtagacc cacgtgttcg cttgcagtaa
    38881 ggagatgtta tggcagataa aaacagaaca tttaaacttg taggtgcagg atcttctagc
    38941 acacaagaaa aaattgaaaa gcctgctctt tcgtttatgc aagatgcgtg gcgtcgcttg
    39001 aaaaaaaaca aattagcagt agtttcactc tatttattag ctcttttact tactttttcg
    39061 ttagcctcaa atttatttgt aactcagaag gatgctaatg ggtttgattc gaaaaaagta
    39121 acgacatatc gcaacttacc acctaaattg agttcaaacc ttcctttttg gaatggtagc
    39181 attaagtacg ctggaaatac agaatctaca gacgcttata agtctcaaaa tgtccctgaa
    39241 aaagttaagt atgctttagg tacagattct ctagggagaa gtgttgctaa gcgcatcatc
    39301 gtaggtattc gaatttcctt acttgtagct attgcagcta cattcattga tttaattatc
    39361 ggtgttacat acggtcttgt ttcaggtttt gctggaggac gccttgatac attaatgcaa
    39421 cgtgtcgtag aggtaatttc atcaattcca aacttagtta ttgttacaat gttaggtttg
    39481 gtccttggta acggtattac ggctattatt atctctattg cttttaccgg atggacttct
    39541 atgtctcgtc aggtaaggaa tttaacccta tcatatcgag aaagagagtt tgtgcttgct
    39601 gcacgttcac ttggggaaag tccaattaaa atagccttca aacatatttt acctaacatt
    39661 tctggtatta tcattgtaca aattatgatg acaatcccaa gtgctattat gtatgaagcg
    39721 gtactttcag caatcaattt aggtgttaaa ccaccgacag cttcattagg atcattgatt
    39781 tcagatgcac aagaaaattt acaatattat ccatatcaag ttatcttgcc agctttagca
    39841 ttggtaatga tttcattggc atttattttg cttggtgatg ggttgcgaga tgcttttgat
    39901 ccaaaatcta gcgatgaata aggaggtata atcatggaaa aagaaactat tttaagtgtt
    39961 aataatcttc atgttgactt ccacacatat gctggagaag taaaagcaat tcgtgatgtc
    40021 aactttgaat taaaaaaagg tgagactctt gcaatcgttg gtgaatctgg ttcaggaaaa
    40081 tctgtaacta ccagaacttt aattggttta aatgctaaaa attcagagat atcagggaat
    40141 gttcaattta aggggcgtaa ccttgttgaa ctatcagaag aagagtggac taaggtacgc
    40201 gggaatgaga tttctatgat tttccaagac cctatgacta gtttggatcc aacgatgaaa
    40261 attggtatgc aaatagcgga gccaatgatg attcatcaaa aaatttcaaa aaaggatgct
    40321 ttaaaattag ctcttgagct aatgaaagat gtaggtatcc caaatgctga agagcatatt
    40381 aacgattacc cacatcagtg gtctggagga atgcgtcaac gtgcggttat agcgattgct
    40441 ttagctgctg atcctgaaat tcttattgct gatgaaccaa cgactgcttt agatgtaaca
    40501 attcaagcac aaattttaaa cttaatgaaa aaaattcaag cagaacgaga ctcctccatt
    40561 gttttcataa ctcatgactt aggggtcgtt gcagggatgg cagaccgtgt agcagttatg
    40621 tatgcaggga aaatcgttga atttggaact gttgatgaag tcttttataa tccacaacat
    40681 ccatatactt ggggattatt gaattcaatg ccgacaaccg aaacagaatc aggtagttta
    40741 gagtcaattc cgggaacacc accagatttg ttgaacccac ctaaaggaga tgcatttgca
    40801 gctcgtaatg aatttgcatt agatattgat catgaagaag aaccaccgta cttcaaagtt
    40861 tcagaaacac atttcgctgc tacatggctt ttagatgaaa gatcacctaa ggtgcttcct
    40921 ccattaccga tccaaaaacg ttgggaaaaa tggaacgaga ttgaagggag gaaagcccaa
    40981 tgactgaaaa tcgaaaaaaa ttagttgaag tcaaaaatgt ttctttgacc ttcaataaag
    41041 gaaaagctaa tgaagtgaga gcaattgata atgttagttt tgacatttat gaaggtgaag
    41101 tgtttggatt agttggggaa tctggatcag gaaagacaac cgttggacgt tcaattttaa
    41161 aactttatga tatttctgat ggcgaaatta cctttaatgg agaagtgatc tcacatctga
    41221 aaggtaaagc attacatagt tttcgtaaag acgctcagat gatttttcag gatcctcagg
    41281 ctagcttaaa cggccgtatg aagattcgtg atatcgtagc agaaggttta gacatacata
    41341 aattagctaa atcaaaatca gatcgtgata gtaaagttca ggccctatta gatcttgtag
    41401 ggttaaataa agatcattta acacgttatc cgcatgaatt ttcaggtgga caacgacaac
    41461 gtatcgggat tgcacgtgct ttagcagtag agcctaaatt tatcattgct gatgaaccaa
    41521 tatctgcctt agatgtttca atccaagcac aagttgttaa tttaatgcaa aaattgcaaa
    41581 gagaacaagg attgacttat ttgtttatcg cacatgattt gtctatggta aaatacattt
    41641 cagatcgtat cggtgttatg cattggggga aactgttaga agttggaaca tctgatgatg
    41701 tttataacaa accaatccac ccttatacga agagtctatt atcagctatt ccagaaccag
    41761 atccggagag tgaacgtcaa cgtgttcacc agccgtataa tccagctatt gagcaagacg
    41821 gacaagaacg tcaaatgcac gagattacac ctggtcattt tgttttatct acaccccaag
    41881 aagctgaaga atataaaaaa caaatattat aaaagatggc tagcccatct tttatttttt
    41941 tactaataaa ataaatttag gtatggaatt ttaaactcta tgttattggt gttagccaac
    42001 aggaaact
//
LOCUS       DATRUK010000013        37351 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.013, whole genome shotgun sequence.
ACCESSION   DATRUK010000013 DATRUK010000000
VERSION     DATRUK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37351
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.013"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..769)
                     /locus_tag="VBE34_000606"
     CDS             complement(<1..769)
                     /locus_tag="VBE34_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO7263346.1"
                     /translation="MVRLIFSDIDGTLINSNFKVTPKTRQGIKQIVAQGATFVPISAR
                     MPEAITPIMEQIGIDSYIISYNGALIQDMQQKTIASHTMDGQVALQVCSYVSKHYSKI
                     AWNVYRYHEWYSCDKENEWVQKEEEIVGLQSKEMSLMELEKQDRIHKLLLMGEPSLMG
                     ELENTLKAQYPHLSIAQSAPYFIEIMAPGIEKGKSAKTLADYLDISLADSIAFGDNYN
                     DLNLLEIVGKGFVMGNAPKDLQERIGNVTQDNDNDGIY"
     gene            955..1815
                     /locus_tag="VBE34_000607"
     CDS             955..1815
                     /locus_tag="VBE34_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7263347.1"
                     /translation="MPYPYKANHSIESITLKVNDLENLVNFYSDIIGLTVIDKSSTRA
                     LLGVNQKIPLIILEKTELEKHSTYGLYHTAILVPDEYHLSLALNHLLSQHIPLEGGAD
                     HGYSNAIYLSDPEGNGIEIYNDKDISMWDIRESGQIIGITERLDIDNLLDSLVNVPNN
                     YKLSEKTRIGHIHLSVKDAKISSKLYQNVFGLDEKFAIPTASWIASGNYHHHLAFNNW
                     AGPNLSKNQENRPGISLLTIAYNDDNLFRDSLKKAQLYQLTFLEKQDHYYIIEDFDGI
                     RIKVVLKPEN"
     gene            1858..2589
                     /gene="yaaA"
                     /locus_tag="VBE34_000608"
     CDS             1858..2589
                     /gene="yaaA"
                     /locus_tag="VBE34_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEO7263348.1"
                     /translation="MIKILIPTAKEMKVCQNIARPKLSAQTQIIIDYFSTLTVSDLED
                     IYRINTSAARCEAQRWQDFKCKQLTLNPAIKLFNGLMYRNIKRHNLSTSEAQFMENSV
                     FITSALYGIIPAMTLISPHRLDFNTKIKINNNSLKVFWRENYDTFMQSDDIMVSLLSN
                     EFKTVFSPKERQKLIHLNFIEDRDGQLKTHSTISKKARGKCLTSMMENNCQTLEHLKQ
                     LRFDGFCYDNELSDSKQLTFVKKQT"
     gene            complement(2946..3563)
                     /gene="nrdG"
                     /locus_tag="VBE34_000609"
     CDS             complement(2946..3563)
                     /gene="nrdG"
                     /locus_tag="VBE34_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEO7263349.1"
                     /translation="MSEEASWNTPKPGEWKSEELSHGHIIDYKAFNFVDGEGVRNSLY
                     VAGCMFHCKGCYNTATWSFRAGIPYTKELEDQIMKDLAQPYVQGLTLLGGEPFLNTGI
                     LLPLLQRIRRELPEKDIWSWTGYTWEEMMLETQDKLEMLSLIDILVDGRFDQSKRNLM
                     LQFRGSSNQRIIDVQKSLKEGEVVIWEGLNDGDNSYEQVKRDYLL"
     gene            complement(3636..4127)
                     /locus_tag="VBE34_000610"
     CDS             complement(3636..4127)
                     /locus_tag="VBE34_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7263350.1"
                     /translation="MKLRRPVLEDKEEILAMYKEFQKESSSMDGGFYEPTTHFEDWLD
                     HNLNMELGIGVPDNFVPYIQFVSFDNDNNAIGFLNLRLRLNDTLLEKGGHIGYSIRPR
                     QRGKGYAKEQLKLGIEQAHLKNINEILVTCHVDNDASKSVILANGGILEDCRHQTERY
                     WIT"
     gene            complement(4136..5068)
                     /locus_tag="VBE34_000611"
     CDS             complement(4136..5068)
                     /locus_tag="VBE34_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7263351.1"
                     /translation="MLQLGIVGLGGISQKAYLPYMRQVTGVHWHLFTRQKQILEEVNM
                     LFGSSTAYDSLDSLAEHPLDGVFIHVATSAHFDIAKLFLKKGIPVFMDKPLTEDYTST
                     KALYDLAKDHKTFLMAGFNRRFAPRIMEMKKVEDKNHIRTFKNAVNAPADFQYKLFDM
                     FIHPLDTALFLTNNVVKRGYFVTKRDGNKILQVSVTLETDSEIIEASMNLQSGSRREI
                     IEIESPEVTYSLDDLSNLSVIDGFDRRAIGFGSWASTLEKRGFEPMIDAFIQAITTGV
                     NPISPKSSLLSHFICDQINKANAPFGMLNLKIME"
     gene            complement(5081..5224)
                     /locus_tag="VBE34_000612"
     CDS             complement(5081..5224)
                     /locus_tag="VBE34_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263352.1"
                     /translation="MGKYQLDYKGQAQVQKFHEKHSTGENANQKSRLKDLRKQFLEKA
                     KKK"
     gene            complement(5299..7497)
                     /gene="nrdD"
                     /locus_tag="VBE34_000613"
     CDS             complement(5299..7497)
                     /gene="nrdD"
                     /locus_tag="VBE34_000613"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEO7263353.1"
                     /translation="MMVLERERFMTESDIKVIKRDGRLVSFDKYKIYTALLKASNKVI
                     KMSPLVEAKLEMIADHVIAEIYNRFKDNIKIYEIQNIVEHKLLEANEYAIAQEYINYR
                     TQRDFERSQATDINFSIGKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAQLNYQKHLKDAKEWVIEDKQEDYAR
                     AKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTNWFEREIQKAILKIRIQGL
                     GSEHRTAIFPKLIFTLKKGLNLEENSPNYDIKQLALECATKRMYPDVLSYDKIIDLTG
                     SFKAPMGCRSFLQGWRDANGQDVTSGRMNLGVVTVNLPRVAMESNGDMDKFWEIFNER
                     MSIARDALVYRVERVKEAIPANAPILYQYGAFGERLGKYENVDRLFNHRRATVSLGYI
                     GLYEVASVFYGGDWEDNHQAKAFTVDIVRKMKQLCADWSDEYDYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKNPTPFEKLDFEKIYPETGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYQCQFEGDFTPTDRGFTCPNCG
                     NSDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSSIKNQGN"
     gene            complement(7596..9137)
                     /locus_tag="VBE34_000614"
     CDS             complement(7596..9137)
                     /locus_tag="VBE34_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263354.1"
                     /translation="MVENLEKPIGVSYKNSPSMSKRTAIRMKKSSRFSILLYSVLSTL
                     LAIANPLLTYFANGLQTQNLYTGLMMTKGQIPYSDVFATGGFLYYVTIALSYLLGSSI
                     WLFIVQFIAYYVSGIYFYKLVYYVAQSEIVSIGMTLIFYIMNIVLGFGGMYPIQWALP
                     FMLISLWFLIKFCVDNIVDEAFIFYGILAAFSLFIDPQTLIFWLCSFVLLTATNIKQK
                     QSLRGFYQFLCVVFGMILIAYTVGYFMFNLQIISSYIDKAIFYPFTYFARTNHSFLLS
                     LAIQIVVLLGSGCLFGLWDFIQNRKKASYQIGLNFIACIFIIYAIMAIFSRDFNLYHF
                     LPALPFGLLLTSNKITILYQKVIDRRSHRRQYFSGKSLIVDLFVKKTYYLPLLLVSLL
                     IGLLVYNTYQNVTLSKERRDISHYLTTKIDRDGKIYVWDKVASIYSQTRLKSASQFVL
                     PHINTAQKNNEKILKDELLQHGAKYFILNKNEKLPNELKSDIKKHYQEVPLSNITHFV
                     LYRFK"
     gene            complement(9388..9705)
                     /locus_tag="VBE34_000615"
     CDS             complement(9388..9705)
                     /locus_tag="VBE34_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEO7263355.1"
                     /translation="MAHNHNHGHNHEHQHEVITLVDENGNETLFEILLTIDGREEFGK
                     NYVLLVPAGAEEDEQGEIEIQAYSFTENADGTEGDLQPIPEDSDAEWDMIEEVFNSFL
                     DEE"
     gene            complement(9731..10150)
                     /gene="ruvX"
                     /locus_tag="VBE34_000616"
     CDS             complement(9731..10150)
                     /gene="ruvX"
                     /locus_tag="VBE34_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEO7263356.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEESGNFGFDR
                     LAELVKEYKVDKFVVGLPKNMNNTSGPRVEASQVYGDKITELFNLPVEYQDERLTTVQ
                     AERMLVEQADISRGKRKKVIDKLAAQLILQNYLDRMF"
     gene            complement(10150..10416)
                     /locus_tag="VBE34_000617"
     CDS             complement(10150..10416)
                     /locus_tag="VBE34_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEO7263357.1"
                     /translation="MGFTDETVRFRLDDSNKVEISETLTAVYRSLEEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDL"
     gene            complement(10618..11016)
                     /gene="spx"
                     /locus_tag="VBE34_000618"
     CDS             complement(10618..11016)
                     /gene="spx"
                     /locus_tag="VBE34_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEO7263358.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHQLPYKEQNLGKESLTRDEILE
                     ILTKTESGIESIVSSKNRYAKALNCNIEELSVNEVIDLIQENPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNVENAEARLRAAL"
     gene            complement(11232..12371)
                     /gene="recA"
                     /locus_tag="VBE34_000619"
     CDS             complement(11232..12371)
                     /gene="recA"
                     /locus_tag="VBE34_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEO7263359.1"
                     /translation="MAKKTKKAEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIVEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYSSVRLDVRGNTQIKGTGEHKDHNVGKETKIKVVKNKVAPPFREAF
                     VEIMYGEGISRTGELIKIASDLDIIQKAGAWYSYNGEKIGQGSENAKKYLADNPAIFD
                     EIDHKVRVHFGMTEDDSPVQSELVEEKNEADDLVLDLDNAIEIEE"
     gene            complement(12445..13704)
                     /locus_tag="VBE34_000620"
     CDS             complement(12445..13704)
                     /locus_tag="VBE34_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEO7263360.1"
                     /translation="MRAEIIAVGTEILTGQIVNTNAQFLSEKMAELGIDIYFQTAVGD
                     NEERLLSLLDIASQRSQMVILCGGLGPTEDDLTKQTLAKFLGKSLTVDLLASQKLDRF
                     FASRPQFARTPNNERQAQLVEGSIPLQNLTGLAVGGIVTSKGVQYMVLPGPPSELKPM
                     VMEQVVPILSNNGTKLYSRVLRFFGIGESQLVTILEDIIKNQTDPTIAPYAKIGEVTL
                     RLSTKAENQNEADFKLDSLEKEILALKTLDNRKLKDLLYGYGDNNSMARTVLELLKVQ
                     NKTITAAESLTAGLFQSQLAEFSGASQVFNGGFTTYSMEAKSQLLGIPKKKLQEYGVV
                     SHFTAEAMAQQARQLLKADFGIGLTGVAGPDELEGYPAGTVFIGIATPEGVSSIKVSI
                     GGKSRSDVRHISTLHAFDLVRRALLKI"
     gene            complement(13793..14344)
                     /locus_tag="VBE34_000621"
     CDS             complement(13793..14344)
                     /locus_tag="VBE34_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEO7263361.1"
                     /translation="MKRCSWVNLDNPLYVAYHDKEWGRAVHDDHVLFELLCLETYQSG
                     LSWETVLNKRQEFRQVFHHYNIEKVAAMSDADLEIILQNPRVIRHRLKLFSTRQNARS
                     IILIQKEFGSFDRSIWSFVDNKVQVNSVNNYNDVPASTTLSERLSKDLKKRGFKFVGP
                     TCLYSFIQAAGMVNDHENICDFK"
     gene            complement(14367..14957)
                     /gene="ruvA"
                     /locus_tag="VBE34_000622"
     CDS             complement(14367..14957)
                     /gene="ruvA"
                     /locus_tag="VBE34_000622"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEO7263362.1"
                     /translation="MYDYIKGKLSKITAKFIVVETAGLGYVIYVANPYSFSGYVNQEV
                     TIYLHQVIRDDAHLLFGFHTENEKEIFLNLISVSGIGPTTALAIIAVDDNEGLVSAID
                     NSDIKYLTKFPKIGKKTAQQMILDLSGKFVEASGESATSRKVSSEQNSNLEEAMEALL
                     ALGYKATELKKVKAFFEGTNDTVEQYIKSSLKMLMK"
     gene            complement(14959..16191)
                     /locus_tag="VBE34_000623"
     CDS             complement(14959..16191)
                     /locus_tag="VBE34_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7263363.1"
                     /translation="MKEFFALPKQLQLRELLRFISITVGSAIFPFMAMYYVQYFGNLV
                     TGILIIITQLSGFVATLYGGHLSDAIGRKKVVIVGSLLATIGWAITIAANVPNHITPH
                     LTFVGILIIEIAHQFYSPAYEAMTIDLTNEQNRRFVYTIGYWLVNIAVMLGSGIAGIF
                     YDHHFFELLIVLLIISAICCLVVYFKFDETKPQEGTFKHDKGVLGTFKNYSQVLVDKA
                     FVVYTLGAIGSSVVWLQVDNYFSVNLKQNFEVVSVLGHTITGAKMLSLAVFTNTLLIV
                     LLMTTINKFIENWPLKRQLILGSLICGFGMLFNISLNTFGAILIAMTFFTFGEMIYVP
                     ASQVLRAEMMVEGKIGSYSGFLAIAQPVASVLAGAMVSLSYFTGKIGVQITLTIFMLA
                     GLVLILYATKMKNIEIGK"
     gene            complement(16223..18196)
                     /gene="mutL"
                     /locus_tag="VBE34_000624"
     CDS             complement(16223..18196)
                     /gene="mutL"
                     /locus_tag="VBE34_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEO7263364.1"
                     /translation="MSKIIELPDILANQIAAGEVVERPSSVVKELVENAIDAGSSQIT
                     IEVEESGLKKIQITDNGEGMTSEDAVLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     IASISLMTIKTATEQGKQGTLLVAKGGNIEKQEVVSSPRGTKILVENLFFNTPARLKY
                     MKSLQSELAHIIDIVNRLSLAHPEVAFTLINDGKEMTKTSGTGDLRQAIAGIYGLNTA
                     KKMIEISNADLDFEISGYVSLPELTRANRNYITLLINGRYIKNFLLNRSILDGYGSKL
                     MVGRFPIAVIDIQIDPYLADVNVHPTKQEVRISKERELMSLISSAISESLKQYDLIPD
                     ALENLAKTSTRSVDKPIQTSFSLKQSGLYYDRTKNDFFIDADTVSEPIANFTNLDKSD
                     GSVDNDVKNSVNQGATQSPNIKYASRDQADSENFIHSQDYLSSKQSLNKLVEKLDSEE
                     SSTFPELEFFGQMHGTYLFAQGNGGLYIIDQHAAQERVKYEYYREKIGEVDNSLQQLL
                     VPFLFEFSSSDFLQLQEKMSLLQDVGIFLEPYGNNTFILREHPIWMKEEEVESGVYEM
                     CDMLLLTNEVSVKKYRAELAIMMSCKRSIKANHTLDDYSARHLLDQLAQCKNPYNCPH
                     GRPVLVNFTKADMEKMFKRIQENHTSLRDLGKY"
     gene            18422..18625
                     /locus_tag="VBE34_000625"
     CDS             18422..18625
                     /locus_tag="VBE34_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO7263365.1"
                     /translation="MTQGTVKWFNSEKGFGFISSETGTDVFAHFSEIKVDGFKTLEEG
                     QKVTFDIQDGQRGPQATNINLVK"
     gene            complement(18682..21258)
                     /gene="mutS"
                     /locus_tag="VBE34_000626"
     CDS             complement(18682..21258)
                     /gene="mutS"
                     /locus_tag="VBE34_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEO7263366.1"
                     /translation="MAKPTISPGMQQYLDIKENYPDAFLLFRMGDFYELFYDDAVKAA
                     QILEISLTSRNKNAEKPIPMAGVPYHSAQQYIDVLVELGYKVAIAEQMEDPKKAVGVV
                     KREVVQVVTPGTVVESTKPDSANNFLVAIDSQDQQTFGLAYMDVSTGEFQATLLTDFE
                     SVRSEILNLKAREIVVGYQLTDEKNHLLTKQMNLLLSYEDERLNDIHLIDEQLTDLEI
                     SAAEKLLQYVHRTQKRELSHLQKVVHYEIKDYLQMSYATKNSLDLLENARTSKKHGSL
                     YWLLDETKTAMGTRMLRTWIDRPLVSMNRIKERQDIIQVFLDYFFERNDLTESLKGVY
                     DIERLASRVSFGKANPKDLLQLGQTLSQIPRIKMILQSFNQPELDIIVNKIDTMPELE
                     SLINTAIAPEAQATITEGNIIKSGFDKQLDNYRTVMREGTGWIADIEAKERAASGIGT
                     LKIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGEMLEAREQS
                     SNLEYDIFMRVRAQVESYIKRLQELAKTIATVDVLQSLAVVAENHHYVRPKFNDEHQI
                     KIKNGRHATVEKVMGVQEYIPNSIYFDSQTDIQLITGPNMSGKSTYMRQLALTVIMAQ
                     MGGFVSADEVDLPVFDAIFTRIGAADDLISGQSTFMVEMMEANQAVKRASDKSLILFD
                     ELGRGTATYDGMALAQSIIEYIHDRVRAKTMFATHYHELTDLSEQLTRLVNVHVATLE
                     RDGEVTFLHKIEPGPADKSYGIHVAKIAGLPIDLLDRATDILSQLEADAVQLIVSAPQ
                     EAVTADLNEELDPGKQQGQLSLFEEHSNAGRVIEELEAIDIMNLTPMQAMNAIFDLKK
                     LL"
     gene            complement(21315..21752)
                     /gene="argR"
                     /locus_tag="VBE34_000627"
     CDS             complement(21315..21752)
                     /gene="argR"
                     /locus_tag="VBE34_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO7263367.1"
                     /translation="MNKIERQKRIKRLIQSGQIGTQEEIKLHLKNEGIDVTQATLSRD
                     LREIGLLKLRSPEGKLYYSLSTATSNRFSPALRSYILKVSRASFMLVLNTNLGEASVL
                     ANFIDEKGLPEILGTMAGADTLLVICQNEDIAKVFEKELSVGL"
     gene            21974..23665
                     /locus_tag="VBE34_000628"
     CDS             21974..23665
                     /locus_tag="VBE34_000628"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEO7263368.1"
                     /translation="MDTKHLIASEIQKVVPYMEQSAILSLLETPKNSSMGDLAFPAFS
                     LAKTLRKAPQIIASDIAEQIKSDQFEKVEAVGSYVNFFLDKAAISSQVLKQVLSDGSA
                     YATQNIGEGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLANIFDKIGYHPVKINHLGD
                     WGKQFGMLIVAYKKWGNEEAVRAHPIDELLKLYVRINAEAEIDPSVDEEAREWFRKLE
                     ANDPEATELWQWFRDESLLEFNRLYDKMNVTFDSYNGEAFYNDKMDEVLELLESKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSIYVVGNE
                     QSAHFKQLKAVLKEMDYDWSDDMTHVPFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKDKVAQAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKANFNPSNSDNYSLNDVESWEIIKLIQDFPRIIVRAADNFEPSIIA
                     KFAINLAQCFNKYYAHTRILDEDAEISSRLALCYATATVLKESLRLLGVDAPNEM"
     gene            23753..24061
                     /locus_tag="VBE34_000629"
     CDS             23753..24061
                     /locus_tag="VBE34_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO7263369.1"
                     /translation="MPSEKEILDALSKVYSEEVIQADDYFRQAIFELASQLEKEGMNS
                     LLATKIDSLINQYVLTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(24088..24960)
                     /locus_tag="VBE34_000630"
     CDS             complement(24088..24960)
                     /locus_tag="VBE34_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO7263370.1"
                     /translation="MLKLDLKTKIKEAILIAFGVALYTFGFVKFNMANHLAEGGISGV
                     TLIIHALFGVNPALSSLLLNIPLFILGARILGKKSLLLTIYGTVLMSFFMWFWQQIPV
                     TVPLKNDMMLVAVAAGILAGTGSGLVFRYGATTGGADIIGRIVEEKSGIKLGQTLLFI
                     DAIVLTSSLVYINLQQMLYTLVASFVFSQVLTNVENGGYTVRGMIIITKESESAAATI
                     LHEINRGVTFLRGQGAYSGREHDVLYVALNPSEVRDVKEIMADLDPDAFISVINVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(25068..26012)
                     /locus_tag="VBE34_000631"
     CDS             complement(25068..26012)
                     /locus_tag="VBE34_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO7263371.1"
                     /translation="MKTRRTPLEKKVKYIISVWAKKFGLLHTLKSISREKYAEKISAS
                     LLYGILSSVAVNFFFQPGHVYSSGATGLAQVISAVSKHWFSFEIPVALAFYAINIPLL
                     ILSWRKIGHKFTIFTFITVTVSSIFIQLMPQITLTTDPLINAIFGGLIMGAGVGFSFK
                     SRISSGGTDIISLTIRKKTGRDVGSISFIINGIILLFAGLLFGWKYALYSMVTIFVSS
                     RVTDAIFTKQKKMQAMIVTSKPDCVIKRIHRDLHRGVTCINDAEGTYNHEKKAVLITI
                     LTREEFSDFKYLMLKADPKAFVSVAENVHIIGRFVDDD"
     gene            complement(26002..27753)
                     /gene="aspS"
                     /locus_tag="VBE34_000632"
     CDS             complement(26002..27753)
                     /gene="aspS"
                     /locus_tag="VBE34_000632"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEO7263372.1"
                     /translation="MKRSMYAGRVRSEHIGTSITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEEVAASVMATAESLRSEFVIEVSGVVTAREQANDNLPTGEVELKVQELSV
                     LNTSKTTPFEIKDGIEANDDTRMRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDNLEF
                     IDVETPMLTKSTPEGARDYLVPSRVNQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSDQEIQDIVEGMIAKVMKDTKGLEVSLPFPRMAY
                     DDAMNNYGSDKPDTRFDMLLQDLTEIVKEVDFKVFSEASVVKAIVVKDKADKYSRKNI
                     DKLTEIAKQYGAKGLAWLKYVDNTISGPVAKFLTAIEGRLTEALQLENNDLILFVADS
                     LEVANETLGALRTRIAKELELIDYSKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     AETAHELEGDLAKVRAVAYDIVLNGYELGGGSLRINQKDTQERMFKALGFSAESAQEQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SEQQLEELSLTVESYEN"
     gene            complement(27846..29126)
                     /locus_tag="VBE34_000633"
     CDS             complement(27846..29126)
                     /locus_tag="VBE34_000633"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEO7263373.1"
                     /translation="MKLQKPKGTQDILPGESAKWQYVENVIRNLFKQYHYDEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFHDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PTKMYYIGSMFRYERPQAGRLREFHQVGVECFGSNNPTTDVETIAMGHHLFEDLGIKN
                     VKLHLNSLGSPESRQAYRQALIDYLTPIREQLSKDSQRRLNENPLRVLDSKEPEDKLA
                     VENAPSILDYLDESSQAHFDAVCHMLDALNIPYIIDTNMVRGLDYYNHTIFEFITEIE
                     DNELTICAGGRYDGLVSYFGGPETPAFGFGLGLERLLLILDKQGISLPIENTIDLYIA
                     VLGSEANLAALDLAQSIRHQGFKVERDYLGRKIKAQFKSADTFNAKVIMTLGSSEVDS
                     KEVGLKNNQTRQEVKVSFENIKTDFSSVLKQLGL"
     gene            29346..29528
                     /gene="rpmF"
                     /locus_tag="VBE34_000634"
     CDS             29346..29528
                     /gene="rpmF"
                     /locus_tag="VBE34_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEO7263374.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            29544..29693
                     /gene="rpmG"
                     /locus_tag="VBE34_000635"
     CDS             29544..29693
                     /gene="rpmG"
                     /locus_tag="VBE34_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO7263375.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            29940..30611
                     /locus_tag="VBE34_000636"
     CDS             29940..30611
                     /locus_tag="VBE34_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO7263376.1"
                     /translation="MTNYKNNFKKFMSNKLYVGLASITLLGIGGAIGVSGAALAHNHH
                     SEHMSFEHKHEGHDKHHDHREKYSLKTLKNISTKIDYKTALETAKKEARSGFISQIEL
                     NTKGDTPFYEIKVIDNKIAKKYKIDANSGKILSTTSDNQLNSKDAQLKQPSKDIQTAL
                     QAIQAKYKNAKIKEISLANNKSNKLVYKVKLLDGNTPKIAQFDSDKASFIKANKLNTT
                     HKNHD"
     gene            30696..31367
                     /locus_tag="VBE34_000637"
     CDS             30696..31367
                     /locus_tag="VBE34_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7263377.1"
                     /translation="MKILVVEDEFDLNRSIVKLLKKQHYSVDSASNGEEALQFVSVAE
                     YDVIILDVMMPKMDGFTFLKLLRNKGSQVSILMLTARDAVEDRIAGLDFGADDYLVKP
                     FEFGELMTRIRAMLRRTNRQVSSDDIQIQDITINLSTKQVWRNDNLIDLTAKEYEVLE
                     YLARHRDQVLSRHQIREHVWDYDYYGESNIIDVLIKNLRRKLDNNRDGSLIKTKRGLG
                     YVIPK"
     gene            31351..32715
                     /locus_tag="VBE34_000638"
     CDS             31351..32715
                     /locus_tag="VBE34_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7263378.1"
                     /translation="MLFQSKINYPKKSSIITQITLWYSSFIFILVIGVLIGSFFISKS
                     IAENKSKKNLEAKAVQMSQALAKGHRYEAFEDGIFYSVYDQNGKVIYSGFPKGFKRDL
                     DHQHKHKKKLSLFSMENRTFQYVDIPISGKNQWLRAIRTVDRLDKQLTELLFSLGIVL
                     PLMLIIITVGGYLILKRAFRPIQEITETAQFITQNEDYTKRIITKNNENELTELAAVI
                     NTMLASIESSFVREKQFNNDVSHELRTPVTVILSESEYGKNYAENLSEAKESFEVIHR
                     QSLSMKKLVEQLLELTKAENPLSIQLEPLNFSIMMKQLVSDSSRLLDNTPIHLDSQIE
                     DDLWIIGQQTLLKRLFDNLFSNAIKFTNNHISISLRQSDNQIVFSIKDNGLGISVDDQ
                     SKIWNRFYQVDSARTKDSQSGIGLGLSLVKQIATIHRAKIWVDSKPDDGSQFTLTFPK
                     LKKD"
     gene            complement(32826..34379)
                     /locus_tag="VBE34_000639"
     CDS             complement(32826..34379)
                     /locus_tag="VBE34_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEO7263379.1"
                     /translation="MPEKMTNSENKRSKGFRMPGAFTILFILTIFSVLATWWIPAGSY
                     SKLQFDTASSKLVVTDPNGKTVHVPATQTQLDKMNVKIKIKEFTSGAISKPVSVPNTY
                     KRLKQNPAGIGSVTTSMVNGTIEAVDIMVFIMVLGGMIGVVRKSGAFESGLLALTKKT
                     KGREFLLIFLVSLLMVLGGTLCGIEEEAVAFYPILVPIFLAMGYDSIICVGAIFLASS
                     VGTSFSTINPFSSVIASNAAGISFTEGLSWRTAGCIAGAIFVVVYLHWYAKKIKANPE
                     FSYSYEDRVEFNAKWGMTTNHTPSLFTIRQKIILSLFVISFPLMVWGVMSQGWWFPTM
                     ASSFLAITIIIMFLTATGANGIGERDVVDEFVNGASSLVGVSLIIGLARGINIILSQG
                     YISDTMLYTASKLASHVSGPVFIIVMMFIYFVLGFVVPSSSGLAVLSMPILAPLADTV
                     GIPRSVVVMAYQFGQYAMLFLAPTGLVMATLQMLDMKYSHWLKFVWPVVLFLLIFGGG
                     LLVLQVLIL"
     gene            complement(34473..35399)
                     /gene="arcC"
                     /locus_tag="VBE34_000640"
     CDS             complement(34473..35399)
                     /gene="arcC"
                     /locus_tag="VBE34_000640"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO7263380.1"
                     /translation="MAKIVVALGGNALGNSPEEQLRLVKHTAKSLVALIKKGHEIVVS
                     HGNGPQVGAINLGMNFAAESGQGTNFPFPECGAMSQGYIGYHLQQSLLNELRQEGINK
                     EVATIITQIEVDESDQAFSAPTKPIGTFYDKETSEKIAIEKGYTFVEDAGRGYRRVVA
                     SPEPKKIIEINSIKTLIENDTLVIAGGGGGIPVINKGGYEGIAAVIDKDKSSALLAGE
                     LAADQLIILTAVDYVYTQFGKENQKALTEVNENQMIDYVNQGEFAKGSMLPKVIACMS
                     FLDHNPKGIALITSLNGLEDALDGKLGTRITK"
     gene            complement(35411..36409)
                     /gene="argF"
                     /locus_tag="VBE34_000641"
     CDS             complement(35411..36409)
                     /gene="argF"
                     /locus_tag="VBE34_000641"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO7263381.1"
                     /translation="MKNLRNRSFLTLLDFSTAEVEFLLKLSEDLKRAKYAGIEQQKLV
                     GKNIALIFEKDSTRTRCAFEVAAHDQGAHVTYLGPTGSQMGKKETSKDTARVLGGMYD
                     GIEYRGFSQETVETLAEFSGVPVWNGLTDADHPTQVLADFLTAKECLHKPYKDIRFTY
                     VGDGRNNVANALMIGASIVGMTYHLVCPKELEPDPELLSKCQEIAKTTGASIEITADI
                     AEGVRDSDVLYTDVWVSMGEPDEVWKERIALLEPYRITQEMLNMTENPNVIFEHCLPS
                     FHNIDTKVGYDIYEKYGLKEMEVSDEVFEGPHSVVFQEAENRMHTIKAVMVATLGD"
     gene            36512..>37351
                     /locus_tag="VBE34_000642"
     CDS             36512..>37351
                     /locus_tag="VBE34_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7263382.1"
                     /translation="MLDILSEKHLERRQRIIISAIAIALAAQINISILADGFIMTLSL
                     FILPVFLYFNDDINPILLCLGITFASPIFRGIILSIAGEAEIHQIIEFVLTDMAFYIC
                     YGITFYTIYWHRSYRNKGTFFFSIIICDYFANLVEISFLIKFNNYTITIFATLFAIAL
                     LRAFISCAVAYTYSYLSLLLQKDDHERRYYYFMWSTSAVKSEVYFMQKNIIEIENIMK
                     NAYLLDKELSKYHLPKEYQHLSLDISRDVHEVKKDYQNIIKGLGTYFSVKNESTMALK
                     DIFQ"
BASE COUNT        12154 a         7379 c         5747 g        12071 t
ORIGIN      
        1 aataaatacc atcgttatca ttatcctgag ttacatttcc aattctctct tgtaaatctt
       61 taggagcatt ccccatgaca aaacctttac ccacaatttc aagcagattt aaatcattat
      121 agttatcacc aaacgcaata gagtctgcaa gagaaatatc taaatagtct gctaaagttt
      181 tagcagattt tcctttttca atacctggag ccattatttc aataaagtaa ggagcagatt
      241 gtgcgattga taaatgtgga tattgagctt tcaaagtgtt ctccaattct cccatcagag
      301 atggttctcc catcaagaga agtttatgaa tcctatcttg cttctctaac tccatcaaac
      361 tcatctcttt agattgtaaa ccaacaatct cctcctcttt ttggacccac tcattttctt
      421 tatcacagga ataccattca tgataacgat agacattcca tgcaattttc gaataatgct
      481 tgcttacata agaacaaact tgtaaggcaa cttgaccatc cattgtgtgg gaagcaattg
      541 ttttttgttg catatcttgt attaaagccc cattatagga aataatataa gaatctattc
      601 caatttgttc cataatcgga gtaattgcct caggcatcct agctgatatg ggaacgaaag
      661 tagccccttg agctactatt tgtttaattc cctgccgtgt cttaggtgtt actttaaaat
      721 tactgttaat taaagtacca tcaatatcac taaatattag ccttaccata ttatcctcca
      781 aaatataatt gtacgcgctt actttagtct aacacttaaa tttttgtttc gcaaataaac
      841 ttcgtctggt ctaatgagac tactaatata agctatattt caaaacaaat tagtttattt
      901 ctctcaatga acggtttaat atgttactat atagtaataa taggaggatt aactatgccg
      961 tatccataca aagccaatca ttccattgaa tcaatcacct taaaagtaaa tgacctcgaa
     1021 aacctcgtaa acttctatag cgatatcatt gggttaactg ttattgacaa atcaagcact
     1081 agagccttac taggggtaaa tcaaaaaatc cctcttatta tccttgaaaa aactgaactt
     1141 gaaaagcata gtacctatgg cctttatcac actgctattt tagtaccaga tgaataccat
     1201 ctcagtctag ctctaaatca tttattatct cagcatatcc ctctagaagg tggagctgac
     1261 catggctata gcaatgctat ctatctaagt gatcctgaag gtaatggaat tgaaatttat
     1321 aatgataagg atatctctat gtgggatatc agagagagtg gacaaattat tggtattact
     1381 gaaagactag atattgacaa tttacttgac agccttgtca acgttcctaa taactataag
     1441 ctttctgaaa aaactcgtat tggtcatatt catttgagtg ttaaagatgc taagattagc
     1501 tcaaaactct atcaaaatgt ttttggcctt gatgagaaat ttgcaatccc gacagctagt
     1561 tggattgcat ccggtaacta ccatcatcat ctagccttta ataattgggc tggtccaaat
     1621 ctttctaaaa accaagaaaa tagacccgga atttccttat tgacaattgc ttacaatgat
     1681 gacaaccttt ttagagatag cttaaaaaaa gcgcaacttt accaactgac tttccttgaa
     1741 aaacaagatc attattatat tattgaagat tttgatggca tccgaattaa agttgttcta
     1801 aaaccagaga attaaccatc acttgacttt tgactcttca ttttacataa tgtcaatatg
     1861 ataaaaattt tgatcccaac tgcaaaagaa atgaaagtgt gtcaaaacat tgcaaggcct
     1921 aaactgagtg cacaaacgca aattataatt gattattttt ctactttaac tgtttcagac
     1981 ttagaggata tctacagaat taatacaagt gcagctcgtt gtgaggctca aagatggcaa
     2041 gattttaaat gtaaacaact tactctgaat cctgctatta agttattcaa cggtcttatg
     2101 taccgcaata tcaaaaggca caacttatcc acaagtgaag cacaatttat ggaaaattct
     2161 gtttttatta ctagtgctct ttatggcatc attcctgcta tgacactaat atcccctcat
     2221 cgtttagatt ttaacactaa aatcaaaatc aacaataact ctcttaaagt tttttggcgt
     2281 gaaaattacg atacttttat gcagtcagat gacattatgg tttctttgct ttctaatgaa
     2341 tttaaaaccg tcttttctcc taaagagcgt caaaaactaa tacatcttaa ctttattgaa
     2401 gatcgagatg gccaattaaa aacccactct accatatcaa aaaaagcaag aggaaagtgt
     2461 ttaacttcta tgatggaaaa taattgccaa actctagaac acttaaagca attacgcttt
     2521 gatggcttct gttatgataa tgaactctca gactctaagc aattaacttt tgttaaaaaa
     2581 cagacttaag taactattta tccacaattt attcacacaa aaagcaagag ataatcgtat
     2641 ctcttgcttt ttgacaaact tgtaaggtct aaaaacctct ctatatcaag gtttcaaata
     2701 atattaacaa aaaataattg ttatcaacaa cttgtggata actatgggga tagacagagg
     2761 ataatctgtt tatataaagt tgattactta acagtaatgt cattgtatat acctttagat
     2821 ttcaaaaata cttgttaaat taacaataat aaacgtcaac atcttgtcac agactgtcaa
     2881 atacttataa acaagctgtg gataaacata actttcaatt tctatttaaa agttaatttt
     2941 tgacactaaa gtaaataatc tcgtttgact tgttcataac tattgtcacc atcgtttaat
     3001 ccttcccaaa tcacgacttc accttctttt aaagattttt gtacatcaat gattctttga
     3061 tttgaagatc ctctaaattg tagcattaaa tttcgcttac tttgatcaaa cctaccatcc
     3121 actaaaatat caatcaaaga taacatctcc aatttatctt gagtctccag catcatttct
     3181 tcccaagtat accccgtcca tgaccaaata tctttttctg gcaattcacg tcggatacgc
     3241 tgaagaagtg gcagcaaaat acctgtattt aagaacggct ctccacctaa taacgttaaa
     3301 ccttggacgt aaggttgtgc caaatctttc ataatttgat cttcaagctc ttttgtataa
     3361 gggattccgg ctcgaaagga ccatgtagca gtattataac accctttaca gtggaacata
     3421 catccagcta cgtagagtga atttctaaca ccttcaccat ctacaaaatt aaaagcttta
     3481 taatcaatga tatggccatg acttaattct tcgcttttcc actccccagg ctttggagta
     3541 ttccaacttg cttcttctga catttctaac ctctctattg atatgaaaag aaccagaact
     3601 aactggtctt tttattatta tcttaaagta ctatcttagg ttatccaata acgctctgtc
     3661 tgatgacgac aatcttctaa aataccaccg ttagctaata ttactgactt gctggcatca
     3721 ttgtctacat ggcatgtcac aagtatttca ttgatatttt ttagatgtgc ttgctcaatt
     3781 cctagcttca actgttcttt agcataacct ttaccacgct ggcgaggtcg tatggaataa
     3841 ccaatatgtc cacctttttc taaaagagta tcatttagtc ttaaacgtaa atttaagaaa
     3901 cctatagcat tattgtcatt gtcaaaagac acaaactgaa tatatggaac aaaattatca
     3961 ggaacaccta tccctaactc catatttagg ttatgatcta gccagtcttc aaaatgcgta
     4021 gttggttcat aaaaaccacc atccattgaa ctactttctt tttgaaactc tttatacatg
     4081 gctaaaattt cttctttatc ttctaacact gggcgccgta atttcatatt gtttcctatt
     4141 ccattatctt caagttaagc attccaaaag gagcattcgc tttattaatc tggtcacaaa
     4201 taaaatgact caataaacta gactttggtg atataggatt gacacctgta gtgatagctt
     4261 gaataaaagc atcaatcatt ggttcaaaac cacgtttctc tagagtgctt gcccaagaac
     4321 caaaaccgat agcacgacgg tcaaagccat caataacaga aagatttgac aaatcatcta
     4381 atgaataggt tacttctggc gactctattt caattatttc tcgacgagac cccgactgca
     4441 ggttcatgct tgcttctatt atctcactgt ctgtttctag cgttactgat acttgtaata
     4501 tcttattccc atcacgttta gtcacaaagt atcctctttt aacaacatta tttgtcaaaa
     4561 atagcgccgt atctaaagga tggataaaca tatcaaaaag cttatattga aaatccgctg
     4621 gtgcattgac agcattttta aaggtgcgaa tatggttttt atcttctact tttttcattt
     4681 ccataatacg tggtgcaaag cgacgattaa aacctgccat taaaaatgtt ttatgatcct
     4741 tcgccaaatc atacaatgct ttagttgaag tataatcttc tgtcaaaggc ttatccataa
     4801 aaactggaat accctttttt aaaaaaagct ttgctatatc aaagtgagca ctagtcgcga
     4861 catgaatgaa gactccatcc aaaggatgct cggctagact atctaaactg tcataagctg
     4921 tagaagaacc aaaaagcata ttaacttctt ctaaaatctg tttttggcgt gtaaaaagat
     4981 gccagtgcac acctgtcact tgacgcatat aaggtagata agctttctgt gaaatacctc
     5041 ctaaaccgac aattccaagt tgcaacatag catcctcctt ctattttttc tttgcctttt
     5101 ccaaaaattg tttccttaaa tcttttagac gtgatttttg attagcattc tctccagtag
     5161 agtgcttctc atggaacttc tgtacttgtg cttgcccttt gtaatctaat tggtatttcc
     5221 ccatatctta ataactcctt ctattttatc ttcttatttt aacatataat agaccagagg
     5281 ttttaataga ggttttaatt aattaccttg atttttaata cttgatccgt tcatatgttt
     5341 tacacgagca gagatttcct tatgacgacc attaaccatc ggacgagcct gtggattacc
     5401 taagtaacca catgtacgtt tgacaacatc tactgtctta gggtcgctgt taccacaatt
     5461 tggacaagta aagcctcgat ctgtcggtgt aaaatcccct tcaaattgac attggtaaca
     5521 cttatcaatt ggtgtattag tacctagata acctacccga tcataagcat agtcccaaac
     5581 tgcttctaaa gcttttggat tctgttgcaa aacagggtat tcacagtagt gaataaatcc
     5641 tcctgatgca cccgtttcag gatagatttt ctcaaaatct agtttttcaa aaggtgttgg
     5701 attcttacgc acatcataat ggaatgaatt agtgtagtat tccttatcag taatatcagt
     5761 tactatacca aatttttcag tatctaaacg acaaaaccgg tcagtcaagc tctccgatgg
     5821 tgtcgaataa actgaaaaat gataatcata ctcatcagac caatctgcac ataactgctt
     5881 catcttacga acgatgtcta cagtaaatgc tttagcttga tgattatctt cccagtcacc
     5941 accataaaaa acactcgcca cctcatataa accaatataa ccaagagaca ccgttgcacg
     6001 gcggtgatta aagagtcgat caacattctc atattttcct aaacgttctc caaatgctcc
     6061 atactggtaa agaatgggcg catttgctgg gatagcctct ttgacacgtt ccacacgata
     6121 aaccaaggca tctctagcaa tactcatacg ttcattaaat atctcccaga atttatccat
     6181 atcaccgtta gactccatgg caacacgcgg tagattaact gttacaactc caagattcat
     6241 acgaccagat gttacatcct gaccatttgc atctcgccac ccttgtagga aggaacgaca
     6301 gcccataggg gctttaaaag agcccgttaa gtcaataatt ttatcataag aaaggacatc
     6361 tggatacatc cgttttgtcg cacactctaa tgctaattgt ttaatatcat aattggggct
     6421 attttcttct aaattaagac cttttttaag cgtaaaaatt aattttggaa aaatagcagt
     6481 acgatgctcg cttcctaaac cttgaatccg aattttcaaa atcgcttttt gaatttctcg
     6541 ttcaaaccaa ttagttccca atccaaaacc aagcgaagta aatggcgttt gcccatttga
     6601 agtaaataaa gtattaatct catactccaa agattgcatg gcatcatata tatctttttg
     6661 tgttttagcg cgcgcatagt cctcttgttt atcttcaata acccattctt ttgcatcttt
     6721 taaatgtttt tgataattta actgtgcata aggagctaaa aactcatcaa tgcgatctgc
     6781 tgtacagcca ccatattgac ttgaagcaac gttcgcaata atctgtgaaa tctgagcagt
     6841 cgctgtctga atagattttg gactctcaac ttctgcatta ccaatcttaa aaccatttgc
     6901 aagcattccc ttaaaatcga ttaaacagca atttgtcatg ggagtgtatg ggctgtaatc
     6961 taaatcatgg taatggatat cacccttttg atgtgcattt gctacatgag aaggtaacat
     7021 tttcagacca attgatttcc ctacaatacc tgctgttaaa tcacgttggg tattaaacac
     7081 atcagagtct ttattagcat tctcattaac aacagtttga tctttattaa ttaattttcc
     7141 aatagaaaaa ttgatatctg tagcctgtga acgctcaaaa tcgcgttggg tacgataatt
     7201 gatatattct tgtgcaatag cgtactcatt agcttccaat aatttatgtt caacgatatt
     7261 ttgtatttca tatatcttga tgttatcttt aaaacgattg tagatctcag cgatgacatg
     7321 atctgcaatc atctcaagtt tagcctcaac taaaggagac attttgataa ctttattact
     7381 agcttttaat agagccgtgt agattttata cttatcaaag ctgaccaaac gcccgtcacg
     7441 tttaatcact ttgatatcag attctgtcat aaatcgttct ctttccaata ccatcatgtg
     7501 aaatatcctc cagtgacaat tatagcatgc aaagaaaaaa aatcaatata ttgtgtcaag
     7561 ttttttgctc agacacaata tattgatttt ttaatctatt tgaaacgata tagaacaaaa
     7621 tgagtgatat ttgatagagg aacttcttgg tagtgcttct taatatcaga tttcagttca
     7681 tttggtaact tctcattttt atttaagata aagtatttag ccccatgctg aagcagctcg
     7741 tccttcaaaa ttttctcatt atttttttgc gctgtattaa tatgtggcaa aacaaattgc
     7801 gaagcagatt ttaaccgagt ctggctataa atgctagcaa ctttgtccca aacataaatc
     7861 ttaccatctc tatcaatttt tgtagtcaaa taatgtgaaa tgtcacgcct ttctttacta
     7921 agagtaacat tttgataagt attataaaca agtaacccta tcaataagga aactagtaag
     7981 aggggcaaat aatatgtttt ctttacaaaa aggtctacga taagagattt accagagaaa
     8041 tactgacgtc tatgtgaacg cctgtcaatc actttttggt aaagaatagt aattttatta
     8101 ctcgttagca aaagaccaaa tggtaaagca ggtaaaaagt ggtaaaggtt aaaatctctt
     8161 gaaaaaatgg ccattatagc gtaaataata aaaatacagg caataaagtt caagccgatt
     8221 tgataagatg cttttttacg attttgaata aaatcccata gtccaaagag gcaacctgac
     8281 cctagaagga caacaatttg aattgctaaa ctaagaagga atgagtggtt tgtcctagcg
     8341 aaatacgtaa aggggtaaaa aatagctttg tcaatataag aagagataat ctgtagatta
     8401 aacataaagt aaccaacagt ataagcaatt aaaatcatac caaaaacaac gcataaaaac
     8461 tgatagaacc ctcgtagtga ctgcttttgc ttaatattgg ttgctgttaa gagaacaaaa
     8521 gagcaaagcc aaaaaatcag tgtttgggga tctataaata gtgagaatgc tgctaaaata
     8581 ccataaaata taaatgcttc atcaacgata ttatcgacac aaaatttaat taaaaaccat
     8641 agcgaaatga gcataaaagg taatgcccac tgtattgggt acataccacc gaatcctaag
     8701 acaatattca ttatatagaa aatcaacgtc atgcctatcg agacaatttc actttgtgcc
     8761 acataataaa ctaatttata aaaataaatt ccagatacat agtaagcaat aaactgtaca
     8821 ataaataacc agatactaga ccctaaaagg taacttagag caatcgttac atagtataaa
     8881 aatcctcctg tagcaaaaac atcactataa gggatctgtc ctttggtcat catcaaacct
     8941 gtatagagat tctgagtctg caaaccattt gcaaaataag tcaataaagg atttgcaatt
     9001 gctaagagag ttgacaaaac actatatagt aaaattgaaa atcgactaga tttcttcatt
     9061 ctgatagccg ttcgtttact cattgatggg ctatttttat agctcactcc aattggtttt
     9121 tctaggtttt ctaccactat ttttctcctc atatttacat actaccagta tatcagaaaa
     9181 ccttatttag tcaatttagc caacacttga ccgtaacgaa taatatccct aaagtagtgg
     9241 tatcttctag acatatattg tttgagaaat tcttgttctt ctaatgttag ctgagtatct
     9301 gacattaaca tcacctcctt ttactacatc atcactatta ttattcgaaa aaaaaaacga
     9361 ctgaacaatt gctcaatcgc ttcaatatta ttcttcatct agaaagctgt tgaaaacttc
     9421 ctcaatcatg tcccattctg catctgaatc ttctggtata ggctgaaggt ctccttcagt
     9481 tccatcagca ttttctgtaa atgagtaagc ttgaatctca atttcacctt gttcgtcttc
     9541 ttctgcacct gcaggaacga ggagaacata gtttttgcca aactcttcac gtccatcaat
     9601 agttagtagg atttcaaaaa gtgtttcgtt cccattctca tctacaagag tgattacttc
     9661 atgttgatgt tcatggttat gaccgtggtt atgattatgt gccattgttt ctccttttta
     9721 tgccgaatgc ttaaaacatg cggtctaaat aattttgtaa gattagttga gctgctaatt
     9781 tatcgataac tttcttacgc ttaccacgac taatatcagc ttgctcaact agcatacgct
     9841 ctgcttgaac agtcgttaga cgttcatctt gatattcaac tggaagattg aataattcag
     9901 tgattttatc gccatatacc tgactagcct ctacacgagg accacttgta ttattcatat
     9961 ttttaggtag accaactaca aatttatcaa ctttgtactc ttttactaac tctgctaagc
    10021 ggtcaaaacc aaaatttcca gattcttcat cgatcttaat gatttcaaga ccttgtgcag
    10081 tgaaaccgag tggatcacta attgcaacgc caacagtttt cgagccgacg tcaagtccca
    10141 ttattctcat tacaaatcta ttccatttcc ttgtaaataa taacgaacga gctcttctac
    10201 aatctcatca cgttcatatt tacgaatttg attgcgagca tcattgtaac gaggtacata
    10261 agcagggtcc ccactcaata cgtaaccaac aatttgattg atgggattgt aacctttttc
    10321 ttctaatgaa cgataaacag ctgttaatgt ttcactaatt tctactttgt tactatcatc
    10381 taatctaaaa cgaacagttt catctgtaaa tcccatacct acaccttctt tcctttaatt
    10441 ctttcactta ttataactta aataaagtat ttgtacaaga aattgataaa cactatatta
    10501 gaaaatcaaa aagggagcac aagaaaccaa gaggtgattc tttgatttct ttactcccta
    10561 ccttataggt gatttttaca ggttatcatg tgactctcac ttatgcttat ctaaagctca
    10621 aagtgctgca cgaagacgag cttctgcatt ttcaacgtta cgaatagagc gaggtagaaa
    10681 agcacgaata tcatcttcct tataacctac ttgaagacgt ttgtcatcaa ttagaattgg
    10741 actctttaaa atacgtggat tttcttgaat aagatcaata acttcattta cactcaactc
    10801 ctcaatatta caattgagcg ccttagcata acgattttta gaagaaacga tactttcaat
    10861 accactttca gtttttgtta atatttctaa aatttcatca cgtgttagtg attctttacc
    10921 taggttttgt tctttatatg gaagttgatg agcatttagc caagtttttg cttttttaca
    10981 acttgtgcaa cttgaaatag tataaatctt aatcatgtgc cttctcactt tctatcccat
    11041 gatagttata gtttaacata gtcacaaaaa tcaaacatgg gacttaagac ctatttttta
    11101 tcaatttact aataaaaata aatagatttt agggattttt aagcttattt ttagtataaa
    11161 aataagttcc aaccactgtc agaacttatt ttagttacat ttattcaagt tcaagatgta
    11221 tagttgctat tttattcctc aatttcaata gcattgtcca aatctaaaac aagatcatca
    11281 gcttcatttt tttcttcaac gagctcagac tgaactggcg aatcatcttc agtcattcca
    11341 aagtgcacgc gcactttatg gtcaatttca tcaaaaattg ctggattatc tgctaaatat
    11401 tttttggcat tttcagaacc ttgtccaatt ttctcaccat tatatgagta ccatgcacct
    11461 gctttttgga taatatccaa atcactagca attttaatta actcacccgt gcgagaaata
    11521 ccttcaccat acataatttc gacaaaagct tcacggaatg gtggagcaac tttattttta
    11581 acaactttga tttttgtctc tttaccaaca ttatgatctt tatgctcacc agtccccttg
    11641 atttgagtat taccacgaac atctagacga accgatgaat aaaatttcaa agcacgacca
    11701 ccaggtgttg tttcagggtt accaaacata acgcctactt tttcccttag ctggttgata
    11761 aaaattgcga ttgtctttgt cttgttaatg gatgctgaca atttacgcat tgcttgactc
    11821 atcatacgag cttgcaaacc aacgtggctg tcaccaatat caccatcaat ctcagcacga
    11881 ggaacaaggg ctgcaactga atcgacaaca actaaatcaa ctgcgcctga atcaatcaat
    11941 ttacctgcaa tttcaagacc ttgctcacct gagtcaggtt gagacaagag taactcatca
    12001 atattaactc caagagccgc agcataggct gggtcaagag cgtgctcagc atcaataaag
    12061 gcagcaatcc caccttcctt ttgggcttga gctacggcat gaagggcaac cgttgtttta
    12121 ccagatgact ctggtccata aatttcgacg atacgtcctt taggatatcc acctgctcct
    12181 aaagcgatat ctaaagcaag agaacctgaa ctcattactt gaaccttttg ttcagctcgc
    12241 tcacctaagc gcatcactgc gcccttacca aaatcttttt caatattttt caaagcatca
    12301 tctaaggctt ttcgacgttc atcaccgaat ttcttagtaa tttcttctgc ttttttcgtt
    12361 tttttagcca atgtcttctc cttatatttc tatgaatcta ggttgattca aatttagaaa
    12421 tcgttctact aattataaca aatattatat ctttaataaa gccctacgca ccaggtcaaa
    12481 agcatgtaaa gtactgatat gacggacatc tgagcggctc ttccctccaa tcgatacttt
    12541 aatagaagaa acgccttcag gggttgcaat cccaataaaa actgtacctg cagggtatcc
    12601 ttccaactca tcaggaccag caactcccgt taaaccgata ccaaaatctg cttttaaaag
    12661 ctggcgagct tgctgagcca ttgcctcagc tgtaaaatga ctgacaactc catattcttg
    12721 caattttttc ttagggatgc cgagaagttg tgattttgct tccatactat aggtcgtaaa
    12781 gccaccatta aaaacttgcg aagcaccaga aaattctgct aactgagatt gaaataaccc
    12841 tgctgttaag ctctctgcgg cagtaatcgt tttattttgt actttcaata attctaaaac
    12901 agtacgtgcc atactattat tatcaccata gccatataat aaatctttta atttacggtt
    12961 atctaatgtc ttaagagcaa gtatttcttt ttctaagcta tctaatttaa agtcggcttc
    13021 attctgattt tcagctttcg ttgataacct taaagttacc tcaccaatct ttgcatatgg
    13081 tgctatagtt ggatctgtct gattcttaat tatgtcttct aagattgtga ctaactgact
    13141 ctcaccgata ccaaagaatc tcaatacacg tgaatataat tttgtgccat tatttgacaa
    13201 aataggtacc acctgctcca taaccattgg ctttaattca ctaggcggac ctggtaaaac
    13261 catatattgg actcccttag atgtaacgat accaccaaca gctagaccag taaggttttg
    13321 tagaggtatt gagccttcaa ccaattgagc ttgtcgttca ttatttggcg ttctagcaaa
    13381 ctgaggacga cttgcaaaaa aacgatccaa tttttgacta gctaataaat ccactgttaa
    13441 tgatttacct aaaaatttag ccaaagtttg tttcgttaaa tcatcctctg taggtccaag
    13501 tccaccacat aggataacca tttgactgcg ctgactagct atatctaaaa gcgataataa
    13561 acgttcctca ttatctccta cagctgtctg gaaataaata tcaattccca attctgccat
    13621 tttctccgac aaaaattgtg cgtttgtatt aacgatctgt cctgttaaaa tttctgttcc
    13681 tacagcgata atttcagccc tcacaacttc accctcttat ctattcgaaa taatgactac
    13741 attttagcaa aaaaagtagc agaagtgcta cttaaaatat atcacattga tattacttga
    13801 aatcacatat attttcatgg tcattaacca tacccgcggc ctgaataaaa gaatagaggc
    13861 aagttggacc aacaaattta aaaccgcgtt tttttaaatc cttagataag cgttcggata
    13921 aggtagtact tgcaggcaca tcgttgtagt tgttaactga gttaacctgt actttattat
    13981 ctacaaaaga ccaaatggag cgatcaaaac ttccgaattc tttctgaatt aatataattg
    14041 acctagcatt ttgtcgtgtt gagaaaagtt ttagacgatg acgaataacc ctaggatttt
    14101 gtaagattat ctctaaatcc gcatctgaca ttgcagctac cttttcaata ttatagtgat
    14161 gaaaaacttg tctgaactcc tggcgtttat ttaatactgt ttcccaagat aaacccgatt
    14221 gataagtttc taaacatagt aactcaaaga gtacatgatc gtcatgaaca gctctccccc
    14281 actctttatc atggtaagct acatataatg gattatctaa attcacccaa gaacatcgtt
    14341 tcatgtgaaa cctcattact tctaaattat ttcatcaaca tcttcaaact agatttgatg
    14401 tattgctcca cagtatcgtt cgtaccttca aaaaaggctt ttactttttt caattcagtc
    14461 gccttatacc ccaacgctaa caaagcttcc atagcttctt caagattgct attctgctca
    14521 gagctaacct tacgactagt cgcactctca ccagaagctt ctacaaactt ccctgacaaa
    14581 tcaaggatca tctgttgtgc tgtttttttt cctatctttg gaaatttagt caaatactta
    14641 atatcactat tatcaatggc acttacgaga ccttcattgt catcgactgc aataattgct
    14701 aaagcggtcg ttggaccaat tcctgatact gaaattaaat taagaaaaat ttctttttca
    14761 ttttccgtat gaaaaccaaa taaaagatgt gcatcatcgc gaatgacttg atgtaagtag
    14821 atggtaactt cttgattaac gtaacctgaa aaagagtagg gatttgccac atatatcacg
    14881 tatcccaatc cagctgtttc cacaacaata aatttagccg ttattttact caattttcct
    14941 ttaatataat cgtacattct attttcctat ctcaatattt ttcattttgg tagcgtataa
    15001 gattaaaacc agacctgcta acataaaaat ggtcaatgtt atttgaacac ctatttttcc
    15061 tgtaaaatag ctgagcgaaa ccatggcacc tgctaataca cttgcaacag gttgtgcgat
    15121 agctaaaaat ccagaatatg atccaatttt tccctccacc atcatctccg cccttagaac
    15181 ctgactagct ggaacataaa tcatttcgcc aaaggtgaaa aacgtcatgg caatcaaaat
    15241 agctccaaaa gtgtttagag aaatattaaa aagcattcca aaaccacaaa tcaagcttcc
    15301 taatattagt tgacgtttca aaggccaatt ctctatgaat ttatttatag ttgtcattag
    15361 taagacaatt aatagagtgt tcgtaaagac tgctaaacta agcattttag cacctgtgat
    15421 tgtatgtcct aacacagaca ctacttcaaa attttgctta agattgaccg agaaataatt
    15481 atcaacctgg agccagacaa cactggaacc aattgctcct aaagtataaa caacaaacgc
    15541 tttatccact aaaacctgac tataattttt aaaagttcct aaaactccct tatcatgctt
    15601 gaaggttccc tcctgtggtt ttgtctcatc aaacttgaag tagaccacca aacaacaaat
    15661 cgctgaaata ataagaagaa ctattaataa ttcaaaaaaa tggtgatcat agaaaatccc
    15721 agcaatccca gaacctagca ttacagcgat gttaaccaac caataaccta tagtgtaaac
    15781 aaagcgacga ttctgttcgt tcgtcaaatc aatggtcata gcttcatagg caggggaata
    15841 aaattggtga gctatttcaa tgataaggat acctacaaag gtcaagtgtg gcgtaatatg
    15901 atttggaaca tttgcagcta tagttatagc ccaaccaatg gttgccagca gtgaacctac
    15961 aatcacaact tttttcctcc ctatagcatc tgacaaatgg cctccatata aggtcgctac
    16021 aaaccctgat agttgagtta tgatgatcaa aattcctgta actagatttc caaaatattg
    16081 aacataatac attgccataa aaggaaaaat agctgaacca accgtaatgg aaataaaacg
    16141 caataattct cttaactgta gctgttttgg caaagcaaaa aactctttca ctttttctcc
    16201 cttcttccct aacaagtaat tatcaatatt ttcctaaatc cctgaggctt gtatgatttt
    16261 cttggattcg tttaaacatt ttctccatat cagcttttgt aaaattaact aaaacaggtc
    16321 ttccatgagg gcaattataa ggatttttac attgcgctaa ctgatctaat aaatgacgtg
    16381 ctgagtaatc gtctagcgta tggtttgctt taatagatcg cttgcaagac atcataatag
    16441 ccaactctgc gcgatatttt ttcactgata cttcattagt gagcaacaac atatcacaca
    16501 tttcataaac gccagactct acctcttctt ctttcatcca aattggatgt tcccttaaaa
    16561 tgaaagtatt attaccatac ggttccaaga aaataccaac atcttgtaat aatgacattt
    16621 tttcttgtaa ttgaagaaaa tctgaacttg aaaattcaaa tagaaatggt actaacaatt
    16681 gttgaagact attatcaacc tcacctatct tttcacggta atactcgtat tttacacgct
    16741 cttgagctgc atgttgatca ataatataca acccaccatt cccttgagca aataagtagg
    16801 tcccatgcat ttgaccaaaa aattcgagct ctggaaaagt cgaactttct tccgaatcta
    16861 atttttcaac aagtttgtta agtgactgtt tactactaag gtagtcctga gaatgaatga
    16921 agttctcact atcagcttga tctctactag catactttat attaggagac tgcgttgctc
    16981 cttgattaac agagtttttt acatcattgt caactgaacc gtcacttttg tcaaggtttg
    17041 taaagttagc aataggttca gatacagtat ctgcatctat aaaaaagtca tttttagtcc
    17101 tatcgtaata caaaccagac tgttttagtg agaagcttgt ttgtataggc ttatctacac
    17161 ttcgggtact agttttcgct aaattctcta aagcatccgg aatcaaatca tattgtttca
    17221 gactctcaga aattgctgag cttattaaac tcattaattc tcgttcctta gaaattctca
    17281 cctcttgctt agtcggatga acgttgacat ctgctagata aggatctatc tgaatatcaa
    17341 tgacagcaat tggaaatctt ccaaccatca actttgaacc ataaccatct agaattgagc
    17401 ggtttaaaag aaaattttta atgtaacgac cgttaattaa tagggtaata tagtttcggt
    17461 tagctcgtgt caattcaggc aaacttacat aacccgaaat ttcaaaatct aaatctgcat
    17521 ttgaaatctc aatcattttt tttgcagtat ttaaaccata aatacctgca atcgcctgtc
    17581 tcaaatcgcc agtcccacta gttttagtca tttctttacc atcatttatc agtgtaaaag
    17641 caacttccgg atgcgctaga ctgagtctat taacaatatc aataatatga gctagttcag
    17701 actgtaaact cttcatgtat ttcaatctgg caggcgtatt aaagaataaa ttttcaacta
    17761 aaatttttgt tccacgagga cttgaaacaa cttcctgttt ttcaatgttt ccccctttag
    17821 caacaagtaa cgtcccttgt ttcccttgct ctgtagctgt ttttattgtc attaaactaa
    17881 tcgaagcgat tgatggtaga gcttcaccac gaaaacctaa ggtccgaata cgaaaaaggt
    17941 cactctggct tttgattttg gatgtagcgt gacgtcgtaa actgagaacg gcatcctcac
    18001 tagtcatccc ttcaccgtta tccgttattt gaatcttttt aagaccagat tcttcaacct
    18061 ctatggtaat ttggctactt cctgcatcta tggcattttc aactaattct ttaacgacgc
    18121 tactaggcct ttcaacaacc tcaccagcag caatctggtt agctaatata tccggtaatt
    18181 caataatttt agacaagttc atccctcctt tttttatgat tttttgtaac tttttttgtg
    18241 atatcttagc cattatacca tattcaaaat attaatttga caataacaca atttttagtt
    18301 gacatcactc ctattacctg ttagacttag ggagcgtaat aagttttgaa gttgtatttt
    18361 tgtttaacat ttttaccttt tttatttttt acagtaacat ttttagaaag gtaataaaca
    18421 aatgacacaa ggtacagtga aatggtttaa ctcagaaaaa ggttttggct ttatttctag
    18481 cgaaactgga acagacgtct tcgctcattt ttcagagatt aaagttgatg gcttcaaaac
    18541 tcttgaagaa ggacaaaaag taacatttga tattcaagat ggtcaacgtg gaccacaagc
    18601 aacaaatatc aatcttgtta aataattaaa aaagagccta aatgaattat ttagtctctt
    18661 ttttgttgcc taaaggctat tttataataa tttctttaaa tcaaatatag cattcatagc
    18721 ttgcattgga gttagattca ttatatctat cgcttctaac tcctcaataa ccctacctgc
    18781 atttgaatgt tcttcaaaaa gcgataattg tccttgttgc ttcccaggat ctagttcctc
    18841 atttaagtca gcagtaacag cttcttgggg ggctgatacg atcaactgta ctgcatcagc
    18901 ttccaactgt gataaaatat cagttgccct atccaataag tcaattggta aaccagctat
    18961 ttttgcgacg tgtatcccat aagacttgtc cgcaggtcca ggttcaattt tatgtaagaa
    19021 ggtaacttct ccatctctct ctaaagtagc cacgtgtaca ttgacaagcc ttgtcaattg
    19081 ttcagataaa tctgtcaact catggtaatg agttgcaaac attgtttttg ccctaacacg
    19141 gtcatgaata tattctataa tcgattgagc taatgccata ccatcataag tggctgtccc
    19201 tcgacctaat tcatcaaaaa gaatcaaaga tttatcactg gctcgtttta cagcttgatt
    19261 cgcttccatc atttctacca taaaggttga ttgcccagaa attaagtcat cagcagcacc
    19321 aatcctagta aatattgcat caaatacagg caaatcaact tcgtctgccg atacaaaacc
    19381 tcccatttgt gccataataa ctgtcagagc taactggcgc atataggtcg acttaccact
    19441 catatttggt cctgtaatca actggatatc tgtctgacta tcaaaataga tactattggg
    19501 aatatattct tgcactccca tcactttttc aacggttgca tgacgcccat tcttaatctt
    19561 aatctgatgt tcatcattaa atttgggacg aacatagtga tgattttctg caactactgc
    19621 caaactctgt agaacatcaa cggttgcaat agtctttgct aactcttgaa gacgtttaat
    19681 ataagattct acttgagcac gaacacgcat aaaaatatca tattctaaat ttgaagattg
    19741 ctcgcgagct tcgagcattt caccttcaat tttggctagt tctgctgttc catagcgttc
    19801 agaatttttt aatgtcgctt tacggaaaaa atgctccggt actagtgata aattggaatt
    19861 ggtcacatgg aagtaatagc cgtctttttt attataatca attttaaggg taccgatacc
    19921 acttgctgct ctttccttag cttcaatatc agcaatccaa cctgtacctt cacgcatcac
    19981 tgttcgataa ttatccaatt gcttatcaaa tccagacttg ataatgtttc cctcagtgat
    20041 agtagcctgt gcttctgggg ctatcgccgt attaattaaa ctttctaatt caggcatagt
    20101 gtcaattttg ttgacaatga tgtcaagctc aggttgattg aaggactgta aaatcatttt
    20161 aatccgagga atttgtgata aggtctgtcc gagttgcaat agatctttag ggttggcttt
    20221 tccgaaagat actcgacttg ctaggcgttc aatatcatat acacccttta aactttctgt
    20281 gagatcgttt ctctcaaaaa aataatcaag aaacacttga ataatatctt gacgttcctt
    20341 gattcgattc atacttacca aaggcctgtc aatccaagtt ctcagcattc gagttcccat
    20401 cgctgtttta gtctcatcta acaaccagta aagacttccg tgcttcttgc ttgttctagc
    20461 attctccagt aaatctagac tatttttcgt tgcatatgac atttgtaaat agtcctttat
    20521 ttcataatga actactttct gtaaatgact aagttcacgc ttttgtgttc tatgcacata
    20581 ttgtaaaagt ttttccgcag cagatatttc caaatcagtt aactgctcat caatcaaatg
    20641 aatatcatta agtcgttcgt cttcgtatga taaaagcaag ttcatctgct tcgtcagtag
    20701 gtgatttttt tcgtccgtca attgatatcc tactacaatc tcacgagctt ttaaatttag
    20761 tatttcacta cggacggact caaaatctgt taaaagggtt gcctgaaact ctccagttga
    20821 gacatccata tatgctagac caaatgtttg ttgatcttgc gaatcaatcg ctactaagaa
    20881 attattagca ctatccggct tcgttgactc cacaaccgtt cctggggtaa caacttgcac
    20941 tacctcacgc ttgaccactc ccacagcttt tttaggatct tccatctgct cagcaatggc
    21001 tactttgtaa cctaattcaa ctaaaacgtc aatatactgt tgagctgagt gatagggaac
    21061 tcctgccatt gggattggct tttctgcgtt cttatttcga ctagtcaagc taatttccag
    21121 gatttgtgct gcttttaccg catcatcata aaataattca taaaaatcac ccattctaaa
    21181 aagcaaaaaa gcatctggat aattctcttt tatatccaga tactgttgca ttcccggtga
    21241 tatcgttggc ttggccattt ctcactccta actaacgggg ctggctgcca tgctcagctc
    21301 ctcaatcatt atatttataa acctactgat agttcttttt caaaaacttt ggcaatatct
    21361 tcattttgac aaatcaccaa caaggtgtct gctcctgcca ttgttcctaa aatctctggc
    21421 agccctttct catcaataaa gtttgctaaa acgctagcct cccctaaatt agtatttaaa
    21481 actaacataa aacttgctcg cgatactttc aaaatatagg agcgtaaggc tgggctaaat
    21541 cgatttgaag tagctgttga taaactataa tatagcttac cttctgggct acgcaatttt
    21601 aaaagaccaa tctcacgcaa gtctcttgat agagtcgctt gcgtgacatc gattccttca
    21661 ttttttaaat gtaatttaat ttcttcttgt gtgccaatct gccctgattg gattaaacgt
    21721 ttaatacgtt tttggcgttc tattttattc atgttattca atccttttta gttagaatta
    21781 tgcttaccaa attatatcaa aaaaaattgt ctttttcact tagtagtgat atcgtaatca
    21841 ttctgtaaaa aagctataaa gtaggctagt tctttacaga atgcattatg aatttaatca
    21901 acaagttctc gtctattttt cataattatg ttaaaatagg gtgactatat tactaattaa
    21961 aataggagat accatggata caaaacatct aattgcgagt gaaattcaaa aagttgttcc
    22021 atatatggaa caatcggcca ttctttcttt attagaaacc ccaaaaaatt cgagcatggg
    22081 agatttagct ttcccagcat tctctttggc taaaactcta cgcaaagcac ctcaaataat
    22141 tgctagtgac attgctgaac aaattaaaag cgaccaattt gaaaaggtgg aagctgttgg
    22201 atcttacgtt aactttttcc ttgataaagc cgcaatctca agtcaagttt taaaacaagt
    22261 cttatctgat ggttctgcct atgctactca aaatattggt gaaggacgaa atgttgccat
    22321 tgatatgtct agtccaaata ttgctaagcc attctcaatt ggacaccttc gttcaacagt
    22381 tattggtgat agtttagcta atatttttga taaaattggc tatcatcctg ttaaaattaa
    22441 tcaccttggc gactggggta aacaatttgg aatgttaatc gttgcctata aaaaatgggg
    22501 caatgaagag gctgtccgtg ctcatcccat tgatgaactt ttaaaacttt atgtccgtat
    22561 taatgctgaa gccgagatag accctagcgt tgatgaagag gcacgtgagt ggtttcgtaa
    22621 acttgaggct aacgatcctg aagcaactga attatggcaa tggttccgtg acgagtcatt
    22681 attagaattc aaccgtcttt acgataaaat gaatgttaca tttgatagct ataacggcga
    22741 ggcattctac aacgacaaaa tggatgaagt gcttgaactg ctagaatcta aaaacctcct
    22801 agtcgaatct aaaggtgcac aagttgttaa cctagaaaaa tacggtattg aacatcctgc
    22861 cctcattaaa aaatccgatg gagcaactct ctacattaca cgtgacttag ctgctgcact
    22921 ttaccgtaaa cgtacctacg attttgcaaa atcaatctac gttgtaggaa acgaacagtc
    22981 tgcacacttt aaacaactaa aagctgttct taaagaaatg gactacgatt ggtccgatga
    23041 tatgactcat gttccttttg gccttgtaac aaaaggagga gcaaaattat caactcgtaa
    23101 aggaaatgta attctccttg aacctactgt tgcagaagct attaatcgtg cggcatctca
    23161 gattgaagct aaaaacccta acctagcaga taaagataaa gttgctcaag cagttggtgt
    23221 aggtgctatt aaattttacg atttgaaaac tgatcgtaca aatggttatg actttgactt
    23281 agaagcaatg gtatcatttg aaggtgaaac tgggccatat gtacaatatg cacatgctcg
    23341 tattcaatca atcttacgta aagctaactt taacccatct aatagcgata attacagtct
    23401 caacgatgtt gaaagctggg aaatcatcaa actcatccaa gattttcctc gtattattgt
    23461 gcgtgcagca gataattttg agccctctat tattgcaaaa ttcgctatca atttagctca
    23521 gtgcttcaat aaatattacg cccacacacg tattttagat gaagatgctg aaatcagtag
    23581 tcgtctagca ctttgctatg caactgctac agtattaaaa gaatctcttc gcctcctagg
    23641 agttgatgct ccgaatgaaa tgtaagaaat gacattacta agactgtcaa atttatgaca
    23701 gtcttaattt tttatattat aataatagca caataagtta caggagaatg ccatgccatc
    23761 agaaaaagaa atattagatg ctcttagtaa agtttatagc gaagaagtaa tacaagcaga
    23821 tgattatttt cgtcaagcta ttttcgagct cgctagtcaa cttgaaaaag aggggatgaa
    23881 ctcactccta gctacaaaaa ttgactcact tatcaatcaa tacgtcttaa cacatcaatt
    23941 tgatgctcct aaaagcatat ttgatcttag tcgcttggta aaaacaaaag ctagccatta
    24001 caagggaact gctatctcag ctattatgct tggtagtttc ttaagtggag gaccaaaata
    24061 aaaatacagg ccagacagcc tgtattttta tttatcgtaa tttcttcgtc gaattttaaa
    24121 atctgagcta ataacttcat ctacatttat aacagaaata aaagcatctg ggtctaaatc
    24181 agccatgatt tctttcacat cacgcacttc acttgggtta agagctacgt acaagacatc
    24241 atgctctcta cctgagtagg caccttggcc ccgtaaaaaa gttacacccc tattaatttc
    24301 atgcaaaatg gttgctgctg cagattctga ttctttggta ataatgatca ttccacgaac
    24361 agtgtagccc ccattttcaa catttgttaa cacttgacta aatacaaagc tagctactaa
    24421 cgtatatagc atctgttgta aattaatata aacaagtgaa gacgttagaa ctatagcatc
    24481 aataaacaat aaggtttgcc ctaattttat tccagatttt tcctctacaa tacgaccaat
    24541 aatatctgca ccacctgtag ttgcaccata tctaaatact aaaccactac cagttcctgc
    24601 taaaattcca gcagctactg caactaacat catatcgttt ttcaaaggga ctgttacagg
    24661 gatttgttgc caaaaccaca tgaaaaaaga cattaacact gtaccatata tagttaataa
    24721 taaggatttt ttccctaaaa tcctagcccc taatataaat aacgggatat taaggagaag
    24781 tgatgaaagt gctggattaa ctccgaaaag tgcatggata atcaaagtga caccggaaat
    24841 acctccttca gccaaatgat tagccatatt aaatttgaca aagccgaaag tatagagagc
    24901 cacaccaaag gcaattaaaa tagcttcttt tatctttgtt tttaaatcca gtttcaacat
    24961 aggcttaatc cttccttatt caaaaaagct aaagttgttt aaacaactta gcttaataac
    25021 atcataatgt ttccttagtg gcggttgacc aaaagtcata tatacagtta atcatcatca
    25081 acgaatcgtc caataatatg aacattttct gcaacggaaa caaaggcttt tggatctgct
    25141 tttagcatta aatatttaaa atcactaaat tcttcacgtg ttaatatagt tattaaaact
    25201 gcttttttct catgattata agtaccttca gcatcattga tacaggttac accacgatgt
    25261 aaatcacgat ggatacgttt aatgacgcaa tcaggcttac tggtaacaat catcgcttgc
    25321 atcttttttt gctttgtaaa aatggcatct gtaactctac ttgatacaaa aattgtcacc
    25381 atcgagtaca aagcgtactt ccaaccaaat aaaagacctg caaataataa aataatacca
    25441 ttaataataa aagaaatact acctacatct ctgcctgttt tctttctaat tgtcaaactg
    25501 ataatatctg ttcccccact agaaattcta gacttaaagc taaaaccaac accagctccc
    25561 ataattaagc caccaaagat agcattgatt aagggatcag ttgtaagggt gatttgaggc
    25621 attagttgaa tgaagataga gcttacagtt actgtgataa acgtaaaaat agtaaattta
    25681 tgtccaatct tacgccaaga taatatcagc aaagggatat taatggcata aaaagctaaa
    25741 gcaactggta tttcaaaaga aaaccagtgt ttactgacag ctgaaataac ctgagctaaa
    25801 ccagttgctc cacttgaata aacatgtcca ggttgaaaaa agaaattaac agcaacactg
    25861 gatagaatac catacagtaa tgaagccgaa attttctctg catatttttc tcgtgagata
    25921 ctttttaaag tgtgtaagag accaaatttt ttagcccata cactgataat atattttacc
    25981 tttttttcta aaggggttcg tctagttttc ataactttct acagtaagtg ataattcttc
    26041 taattgttgt tctgaaacga gactaggagc ttgagtcatt ggatcactag ccttattgtt
    26101 tttagggaat gcaataactt ctcgaatatt gtccttgcct gctaacaaca taacaaagcg
    26161 gtcaaggcct attgccaatc caccatgtgg tgggaagccg taatccatag cttcaagtaa
    26221 gaaaccaaac tgttcctgag ctgattcagc gctaaatcct aaagccttga acatacgctc
    26281 ttgtgtatct ttttggttaa tacgtaggct accaccgcct aactcatatc cattcaaaac
    26341 aatatcatag gcaactgcac gtactttagc caaatcccct tccaactcat gcgctgtctc
    26401 cgctgttggc aaggtgaatg gatgatgagc cgacatatat cgtccctctt cttctgacca
    26461 ctcaaacatt ggccaatcaa caacccatag gaaattaaat ttagaataat caatgagttc
    26521 aagttctttt gcaatgcgtg tacgaagcgc tcctaaagtc tcgttagcta cttcaagact
    26581 atctgctaca aatagaatta agtcattatt ctctagctgc aaagcttctg tcaacctacc
    26641 ttcaatagct gtcaagaact tagcaaccgg acctgaaata gtattatcta catattttag
    26701 ccatgctaaa cctttggcac cgtattgttt tgcaatttct gttaatttat caatattttt
    26761 tcgagagtac ttgtccgcct tgtctttgac aacaatcgct ttaactactg aagcttctga
    26821 gaacaccttg aagtcaactt cttttacaat ttctgtcaag tcttgtaaaa gcatatcaaa
    26881 ccttgtatca ggcttatcag acccgtagtt attcattgca tcatcatatg ccatccttgg
    26941 gaatggtaaa gaaacctcta aacctttagt gtctttcatt actttagcaa tcataccctc
    27001 aacaatgtct tgaatttctt gatcactaag gaatgatgtc tctaagtcaa cttgtgtaaa
    27061 ctcaggttga cgatctccac gtaaatcctc atcacgaaaa catttgacaa tttgataata
    27121 acgatcaaat cctgcattca tcaatagctg ttttgtaatt tgagggcttt gtggaagggc
    27181 ataaaaatgt ccttggttga cacgtgatgg aaccaaatag tctcgtgccc cctctggagt
    27241 tgattttgtt agcatcggag tttcgacatc aataaactct aagttgtcaa ggtaattacg
    27301 gattgagtgt gttactttcg cacgtaattt gaaattttca agcatttccg gacgacgaag
    27361 atccaagtaa cggtaacgca tgcgtgtatc atcatttgcc tcaataccat ctttaatttc
    27421 aaatggtgtt gttttcgagg tattaagaac cgataattct tgcactttta attcaacttc
    27481 tcctgttggt aaattatcat tagcttgttc tcgtgctgtc actacaccgc taacctctat
    27541 aacaaattca ctacggagac tttctgcggt tgccataact gaggctgcta cttcttcagg
    27601 attaataact aattgcatga ttccctcgcg atcgcgaaga tcaataaaaa taaggcctcc
    27661 aaggtcacga cgacgaccaa cccatccttt taaagtaatc gatgttccta tgtgctcact
    27721 acgaacacgc ccagcataca tactacgttt catctatttt ctccatttaa aatattcatt
    27781 ccaacctatt ataacaaaaa gcacctaaaa accccaccca ttaaggagag tttttaggaa
    27841 ctgttttaca atcctaattg tttaagcaca gatgaaaaat ctgttttaat gttttcaaaa
    27901 ctaactttaa cttcttgacg tgtttgatta ttttttaaac cgacctcttt cgaatctact
    27961 tcactgctac ctaatgtcat aatcacttta gcattaaaag tatctgcaga cttaaattgt
    28021 gctttgattt tacgacccaa ataatcacgc tctactttaa aaccttgatg acgtattgat
    28081 tgtgccaaat ctaaggcagc tagattagcc tctgatccta atacagctat atacagatct
    28141 atagtatttt ctattggtaa agaaatccct tgcttatcta atattaagag caagcgttct
    28201 aatcctaatc caaaaccaaa tgctggtgtt tctggtccac caaaatagga tactaagccg
    28261 tcataacggc ctccagcaca gatcgttaat tcattatctt caatctcagt gataaactca
    28321 aaaatagtat ggttataata atcaagacca cgaaccatat tagtatcaat gatgtatgga
    28381 atatttagag catcaagcat atgacacaca gcatcaaagt gggcttggct tgactcatct
    28441 aaataatcta aaatagaagg agcattttca actgctagtt tgtcttctgg ctccttagag
    28501 tctagtacgc gcaatggatt ttcattcaaa cgacgttgac tatccttaga caattgttca
    28561 cgtatgggtg ttaagtaatc aataagagct tggcgatagg cttgacgact ttctggactt
    28621 cctaaactat taagatgaag tttaacgttt ttgataccta aatcttcaaa tagatgatgt
    28681 cccattgcga ttgtttcaac atcagttgtt ggattatttg atccaaagca ctcaacgcct
    28741 acttgatgaa attctcgtaa acgaccagcc tgtgggcgct catatcggaa cattgatcca
    28801 atataataca ttttggttgg tttttgaact tctggtgcaa atagtttatt ttcaacataa
    28861 gaacgtacaa caggtgctgt tccttctgga cgaagcgtaa tgtgacgatc tcctttatca
    28921 tgaaaatcat acatctcttt tgtaacgata tccgttgtat ccccaactga gcgactaata
    28981 acctcgtaat gttcaaacat tggtgtacga atttcatcat aatgatattg tttaaagaga
    29041 ttgcgtataa cattttcaac gtattgccac tttgctgatt ctccaggtaa gatatcctga
    29101 gtcccttttg gtttttgaag tttcataagt ttacctcttc gattagtttt ctctttaaac
    29161 attctaccat tttttcttat attttaaaca agcatatatc atttgcttta gtttgatagt
    29221 attttcacca tgaaattgta aaaataaaaa gttttgtcaa gtaactttac tttacaaaaa
    29281 agatatgtta tcatagtgaa gttgatgaaa atcaaaactt acacatcgaa aggaagaata
    29341 agaaaatggc agtacctgca cgtcacactt caaaagcgaa gaaaaacaaa cgtcgtacac
    29401 actacaaatt gacagctcca tctgtacaat tcgacgaaac tactggagat tactcacgtt
    29461 cacaccgtgt atcactcaaa ggatactaca aaggacgtaa aatcgctaaa gctaacgaag
    29521 ctaaataata gaagggagat accatgcgcg taaatattac acttgaacat aaagaatctg
    29581 gtgaacgctt gtaccttact tcaaaaaaca aacgtaacac tccagaccgt cttcaattga
    29641 aaaaatactc accaaaactt cgtaaacacg ttgtttttac tgaagttaaa taattgatta
    29701 taaaaaagcc tatgaaatca atatttctgg gctttttatt tttaaaaata aaatctgaca
    29761 aaaaagtata aatttacctc ttaccttcag gtagttagtc attcttttaa aactcacttt
    29821 tttataactg aaaatattgt tcttaatttt aaaaattcat tttagattca ttttacgact
    29881 atatactcag aagtaacaaa cctaatccaa ggtttgaaaa aagaaagaag gaagtcagta
    29941 tgacaaacta taaaaacaac tttaaaaaat tcatgagcaa taaattatac gtagggttag
    30001 cttccattac gctacttggt attgggggag ctataggagt aagtggagct gcattagcgc
    30061 ataaccatca ctcagaacac atgtcttttg aacataagca tgaaggacac gataaacatc
    30121 acgatcatag agaaaaatac tcactaaaaa cattaaagaa tatctcgaca aaaatcgatt
    30181 ataagactgc cttagaaact gccaaaaaag aagctcgctc aggctttatt tcacaaattg
    30241 aattaaatac caaaggtgat acaccttttt atgaaatcaa agttattgat aataaaatag
    30301 ctaaaaaata taaaattgat gctaattctg ggaagatcct ttcaacaaca tcagataatc
    30361 aactcaattc aaaagatgcc caattaaaac aaccaagtaa agatattcag actgcattac
    30421 aagctattca agcaaaatat aaaaatgcaa aaataaaaga aatttctctt gcgaataaca
    30481 aatcaaataa gctagtttat aaagtaaaac tacttgatgg aaatactcct aagattgccc
    30541 aatttgatag tgataaagca tcatttataa aggcaaataa gcttaataca actcataaaa
    30601 atcacgacta atactagtgt attaaaggga ataggacctc agtactccat tcccttttgt
    30661 gatataatgg aaaaaagcca tacggaggcc aaaaaatgaa aatccttgtt gttgaagatg
    30721 agtttgactt gaaccgtagt attgtaaaac ttttaaaaaa gcaacactac agtgttgaca
    30781 gtgcttcaaa tggtgaagaa gccttacaat ttgtttcagt ggcagaatat gatgtgatta
    30841 tcttagatgt aatgatgcca aaaatggacg gcttcacctt tttaaaactg cttcgcaaca
    30901 aaggaagtca agtatctatt cttatgttga ctgctcgaga tgccgttgaa gatcgtatcg
    30961 caggtctaga ttttggtgcg gatgattacc ttgtaaaacc atttgaattt ggagaactca
    31021 tgacccgaat tcgagctatg ttacgacgta caaatagaca agtatcttct gatgatattc
    31081 aaattcaaga tataacaatc aatttatcta caaagcaagt ttggagaaac gacaatttga
    31141 ttgatttaac agctaaggaa tacgaagtcc ttgagtattt ggcacgtcac agagaccaag
    31201 tcctctctcg tcatcaaatt cgtgaacacg tttgggatta tgattattat ggagagtcca
    31261 atattattga tgtccttatc aaaaaccttc gtcgaaaact agataacaat cgagatggat
    31321 cactaataaa aactaaacga ggtttaggat atgttattcc aaagtaaaat aaattaccct
    31381 aaaaagtcta gcatcatcac tcaaattacc ctctggtaca gctcttttat ttttatcctt
    31441 gttatcggtg tattaattgg ttcatttttt atttcaaaaa gcattgctga aaacaaatct
    31501 aaaaaaaatt tagaagctaa agctgtgcaa atgagtcaag cgttagctaa aggtcatcgg
    31561 tatgaggctt ttgaagatgg aatattttat tcagtctatg accaaaatgg aaaagttatc
    31621 tattctggtt ttccaaaagg atttaaaagg gatttagacc atcaacataa acataaaaag
    31681 aaattatctc tattttccat ggagaatcgt acctttcagt atgttgatat tccaatttct
    31741 gggaaaaatc agtggttacg tgctatccgt actgttgaca gactggataa gcaattaact
    31801 gaattgttat ttagtttggg tatcgttctt cctcttatgt tgattataat aactgttggt
    31861 ggctatttaa ttttaaagcg cgcttttaga ccgattcaag aaattactga aaccgctcaa
    31921 tttattaccc aaaatgaaga ctatactaag cgaattatca ctaaaaataa cgaaaatgaa
    31981 ttaactgagc tagcagctgt tatcaatact atgttggctt ctattgaatc atcatttgtt
    32041 cgggaaaaac aatttaacaa tgatgtttct catgaactac ggactcctgt aacagttata
    32101 ctttctgaaa gtgaatacgg aaaaaactac gcagaaaatt taagtgaagc aaaagaatcc
    32161 tttgaggtta tacaccgtca gtcactatca atgaaaaaat tagttgagca acttctagaa
    32221 ttgactaagg cagaaaatcc attatcaatt cagttagaac ctcttaattt ctcaattatg
    32281 atgaagcaac tagtatccga ttcgagtaga ttattagata atacccctat acaccttgac
    32341 tctcaaatcg aagatgacct ttggattata ggtcaacaaa cactactcaa acggcttttt
    32401 gataatctat tttcaaatgc tattaaattt acaaacaatc atatttctat tagtctaaga
    32461 cagtccgata atcaaattgt cttctctatt aaagataatg gtctaggtat ctcagttgat
    32521 gaccaaagta aaatttggaa ccgtttctat caagtggact ctgctcgaac caaggatagt
    32581 cagtctggaa ttggtttagg gctatcttta gtaaaacaaa tcgctacaat acaccgtgct
    32641 aaaatatggg ttgattctaa acctgatgat ggcagtcagt ttacgctcac ctttccaaaa
    32701 ttgaagaaag actagagaaa ggaaataaaa aaaacgatag attacaccta tcgttttttt
    32761 ttatttaact taaactcaat ttattatatc tcttcagata tttttgagac taatagctga
    32821 aatctttaaa gtattaaaac ttgtagaact aacaaaccgc caccaaatat taggaggaat
    32881 aaaacaaccg gccacacaaa ttttagccaa tgggagtact tcatatctaa catctgcaag
    32941 gttgccataa ctagtccagt aggtgctaaa aacaacattg catactgccc aaattgataa
    33001 gccattacta ctactgaacg aggaatccca acagtatctg caagaggtgc tagaattggc
    33061 attgaaagta ctgctaaacc cgacgaggat ggaacaacaa atcctaacac aaagtagata
    33121 aacatcataa caatgataaa gacagggcca ctaacatgtg aagctaattt tgaagctgtg
    33181 taaagcatcg tatctgatat ataaccttgg ctcaaaataa tattgatacc acgcgctaaa
    33241 ccaatgatta atgaaacccc aacaaggctt gatgcaccat taacaaactc atcaacaaca
    33301 tccctttctc caataccatt tgccccagtt gctgttaaaa acataataat aattgtaata
    33361 gctaaaaagg aagaagccat ggttggaaac caccatccct gtgacattac tccccaaacc
    33421 atcaaaggaa atgaaataac aaataaactt aaaataattt tttgtctaat agtaaagagt
    33481 gagggtgtat ggttagttgt catgccccat ttagcattaa attctactct atcttcatag
    33541 gaatatgaaa actctggatt cgcttttatt tttttagcat accaatgtag atacaccaca
    33601 acaaaaatag cacctgcaat acaccctgct gtacgccatg ataagccttc tgtgaaacta
    33661 ataccagctg catttgaagc aataacagaa gaaaacggat taattgttga aaatgatgtc
    33721 ccaacagaac ttgctaagaa aatagcacct acacaaatga tagaatcata acccatggct
    33781 aaaaagattg gaactaaaat aggataaaaa gcaactgctt cttcttcaat accacataga
    33841 gtaccgccta aaaccatcaa taaggaaact aaaaatatca ataaaaactc tctacctttg
    33901 gttttctttg ttaaagctag aagaccagac tcaaaagcgc cactctttct aactactcct
    33961 atcatgcctc caagtaccat gataaatacc ataatatcaa cagcttcaat ggttccattt
    34021 accatactag tcgtcacgga gccaatgcct gctggatttt gcttcaaacg tttgtaagta
    34081 tttggaactg aaactggctt actaatagca ccagaagtaa attctttaat tttaattttg
    34141 acattcatct tatccaactg tgtttgcgtt gctggaacat gaactgtctt accatttgga
    34201 tctgttacta ctagtttgga tgatgctgta tcaaattgta attttgaata ggacccagca
    34261 ggtatccacc atgtagccaa aaccgaaaag attgttaaaa tgaataatat tgtaaaggca
    34321 cctggcattc gaaaaccttt actccttttg ttttcactgt ttgtcatttt ttctggcatg
    34381 acacgcctcc tctataataa aaatagattg acttcatcag aaaaagtgca aagatgaagg
    34441 ttaaataatc atttctttgc acttatctga gattacttag ttatacgtgt tcctaatttc
    34501 ccatctaatg catcttctaa accatttaat gaggtgatta aggctattcc tttcggattg
    34561 tgatctaaga aactcataca ggctattact ttaggtaaca tacttccttt tgcaaactca
    34621 ccttggttaa cgtaatcaat catttgattc tcattaacct ctgttaaagc tttttgattt
    34681 tctttaccaa attgagtata aacataatca actgcagtga gaataatcaa ttgatccgca
    34741 gctagttctc ctgctaacag tgcactagat ttgtctttat caataacagc tgcaatacct
    34801 tcataacctc ccttattaat gacagggatg cctccacctc cacctgcgat aactagggta
    34861 tcattttcaa taagtgtttt aatgctatta atttcgatga tttttttagg ttcaggtgaa
    34921 gcaacaacac gacgataccc tcgtccagcg tcttctacga aagtataccc tttctctata
    34981 gcaatctttt ctgatgtctc cttatcataa aaggtgccta taggcttggt aggtgcgcta
    35041 aatgcttggt ctgattcatc cacctcaatt tgggtaataa ttgtagcaac ttccttattt
    35101 attccctctt gacgtaattc atttaaaagg ctctgttgga gatgatagcc tatgtatcct
    35161 tggctcattg ctccacattc tggaaaagga aaattagttc cttgaccact ctcagccgca
    35221 aaattcattc ctaaattaat ggcaccaact tgtggtccat taccatgact tacaacaatt
    35281 tcgtgtccct tttttatcaa ggccaccaat gattttgcag tatgttttac taatcttaat
    35341 tgttcttctg gagaatttcc taaggcattt cctcctagtg caacaacaat ttttgccatg
    35401 aaaattcctc ctaatcacct aaagttgcta ccataactgc tttaatagta tgcatacgat
    35461 tttcagcttc ttggaaaaca actgagtgag ggccttcaaa aacttcatct gatacttcca
    35521 tttccttcaa accatatttt tcataaatgt cgtatccaac tttagtatct atattatgaa
    35581 atgagggcaa gcaatgctcg aaaataacgt ttggattttc tgtcatatta agcatttctt
    35641 gggtaatacg atagggttct aaaagagcta ttctttcttt ccaaacttca tccggttctc
    35701 ccattgaaac ccaaacatct gtatatagga cgtctgaatc tctgacgcct tctgcaatat
    35761 cagcagtgat ttcaatagaa gcaccagtag ttttcgcaat ttcttgacat ttagagagta
    35821 gttcaggatc tggctctaat tctttaggac aaaccaaatg ataagtcatg cctacaatag
    35881 aagcaccgat catcaaggca ttagcgacat tgttacgtcc atcacccaca tacgtaaagc
    35941 gaatatcttt atagggctta tgtaagcatt ccttagcggt cagaaaatcg gctagtactt
    36001 gcgttggatg atcagcatcg gttaaaccat tccacacagg aactcctgaa aattcggcta
    36061 aagtctcaac tgtttcttga gaaaatccac gatactcaat accatcatac ataccaccta
    36121 gcacacgcgc agtgtctttt gaggtctctt ttttacccat ctgactgcct gttggaccta
    36181 aataagtaac atgagctcct tgatcgtgag cagcaacttc aaaagcgcaa cgtgtccgtg
    36241 tagaatcttt ttcaaaaatc aaagcgatat ttttaccaac aagtttttgt tgttcaattc
    36301 ctgcgtattt agcacgtttt aaatcttctg aaagttttaa aagaaattca acttctgctg
    36361 tactaaaatc caataaagta agaaaactac ggttacgtaa atttttcatt gcttattgcc
    36421 tccttttttt ttatactctt attgtagata gcgctttcaa aaataatcaa aattcatcaa
    36481 aaatcttcaa aaaaacttta aggacattta tatgctagat atattatcag aaaaacatct
    36541 agaaagaagg cagcgtatta ttatctctgc aatagctatc gccttagctg ctcaaatcaa
    36601 tatctcaata cttgctgatg ggtttattat gaccttatct ttgttcattt tgcctgtctt
    36661 tttatatttt aatgacgaca ttaatccaat tcttctctgt ctaggaatta cttttgcttc
    36721 tcctatcttc agaggaatca tattaagtat cgctggcgag gctgagattc atcaaataat
    36781 tgaattcgtt ttaactgata tggcgtttta tatttgttat ggcatcacct tttatactat
    36841 ttattggcat cgttcctacc gtaataaagg tacttttttc ttttctatca ttatttgtga
    36901 ttattttgcc aaccttgtag aaattagttt tctcataaag tttaacaatt atactataac
    36961 tatattcgct actttatttg ccatagcact tttgcgagct tttattagtt gcgctgtggc
    37021 ttatacttat agttatctat cacttctctt acaaaaagac gatcacgaac gtcgatacta
    37081 ttattttatg tggtcaactt cagccgttaa aagcgaggtt tattttatgc aaaaaaatat
    37141 cattgaaatt gaaaacatca tgaaaaatgc ttatttatta gataaagagt tatccaaata
    37201 tcacctacct aaagaatacc aacacctctc tctagatatt tcgcgggacg ttcacgaagt
    37261 taagaaagat taccaaaata ttattaaagg cttaggaacc tattttagtg taaaaaatga
    37321 atcaactatg gcattgaaag atatctttca a
//
LOCUS       DATRUK010000014        35356 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.014, whole genome shotgun sequence.
ACCESSION   DATRUK010000014 DATRUK010000000
VERSION     DATRUK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35356
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.014"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            43..768
                     /locus_tag="VBE34_000643"
     CDS             43..768
                     /locus_tag="VBE34_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7263383.1"
                     /translation="MLKIENVTGGYVNIPVLKNISFEVNDGELVGLIGLNGAGKSTTI
                     NEIIGILRPYQGDITIDGISLEANQELYRKKIGFIPETPSLYEELTLREHLETVAMAY
                     DIATDEVMARAQKLLEMFRLTDKLDWFPMHFSKGMKQKVMIICAFVVSPSLFIVDEPF
                     LGLDPLAISDLINLLAEEKAKGKSILMSTHVLDSAEKMCDRFVILHKGEIRAVGTLEE
                     LRAIFGDSNANLNDIYIALTKEV"
     gene            770..1804
                     /locus_tag="VBE34_000644"
     CDS             770..1804
                     /locus_tag="VBE34_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7263384.1"
                     /translation="MKKLFNKRRSLFLTQNSKYLRYVFNDHFVLVLMFLSGFLLYQYS
                     QLLKDFPKTHWPIIVIVSIIILMLLAMGEIASYLEPADKQFLLIKEEAIKEIINSAKK
                     RTYIFWLVVQTLFLVLISPILIKLGLSVFMITLLIFGLGIIKWLVITYKVKVFYNNQN
                     LNWDAAINHEQERKQAILKFFSLFTNVKGISTSVKRRSFLDVILKLISKTPSRLWTNL
                     FVRAFLRSSDYLGLTIRLVTLNILSVIFVNETYLALALAFVFNYLLLFQLLALGHHFD
                     YQYMNQLYPVRLNAKASQLKGFLRVLSYAVTVIDIILIRELKPVILLIVLMLIVTEYY
                     IPYKIKKMID"
     gene            1862..2662
                     /locus_tag="VBE34_000645"
     CDS             1862..2662
                     /locus_tag="VBE34_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEO7263385.1"
                     /translation="MTISNQELTLTPLRGKSGKAYIGTYPNGERVFVKYNTTPILPAL
                     AKEQIAPQLLWARRTSNGDMMSAQEWLDGRTLTKEDMGSKQIIHILLRLHKSRPLVNQ
                     LLQLGYKIENPYDLLMDWEKQTPIQIRENTYLQSIVTELKRSLPEFRTEVATIVHGDI
                     KHSNWVITTSGLIYLVDWDSVRLTDRMYDVAYILSHYIPQKHWKDWLSYYGYKDNEKV
                     WSKIIWYGQFSYLSQIIKCFDKRDMEHVNQEIYELRKFRELIKKYNAS"
     gene            2652..3287
                     /gene="trmB"
                     /locus_tag="VBE34_000646"
     CDS             2652..3287
                     /gene="trmB"
                     /locus_tag="VBE34_000646"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEO7263386.1"
                     /translation="MRVRKRKGAEEHLENNPHYVISNPEEAKGRWHEIFGNNNPIHIE
                     VGSGKGAFITGMAEQNPDINYIGIDIQLSVLSYALDKVLDSGAKNIKLLLVDGSSLSN
                     YFDTGEVDLMYLNFSDPWPKKKHEKRRLTYKTFLDTYKDILPEQGEIHFKTDNRGLFE
                     YSLASFSQYGMTLKQVWLDLHASDYQQNIMTEYERKFSNKGQVIYRVEARF"
     gene            3331..3418
                     /locus_tag="VBE34_000647"
     tRNA            3331..3418
                     /locus_tag="VBE34_000647"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3365..3367,aa:Ser,seq:gga)
     gene            3776..4252
                     /gene="rimP"
                     /locus_tag="VBE34_000648"
     CDS             3776..4252
                     /gene="rimP"
                     /locus_tag="VBE34_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEO7263387.1"
                     /translation="MANQTIVDIVTQVVTPAIQAPFELVDVEYEKMGGDYVLSILIDK
                     PGGITVEDTAQLTDVVSPLLDTIQPDPFPEQYMLEVSSPGLERPLKTAEALSNAVGSY
                     INVSLYKSIDKVKIFEGDLLSFDGETLTIDYMDKTRHKTVDIPYQTVAKARLAVKL"
     gene            4279..5439
                     /gene="nusA"
                     /locus_tag="VBE34_000649"
     CDS             4279..5439
                     /gene="nusA"
                     /locus_tag="VBE34_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEO7263388.1"
                     /translation="MDTMSKEMLEAFRILEEEKHINKEDIIDAVTESLKSAYKRRYGQ
                     SESCVIEFNEKKADFTVYTVREVVDEVFDSRLEISLKDALAISSAYELGDKIRFEESV
                     TEFGRVAAQSAKQTIMEKMRRQMREVTFNEYKQHEGEIMTGTVERFDQRFIYVNLGSL
                     EAQLSHQDQIPGESFKSHDMIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEREIPEV
                     FDGTVEIMSVSREAGDRTKVAVRSHNSNVDAIGTIVGRGGSNIKKVISNFHPKRVDAK
                     TGLEIPVEENIDVIQWVEDPAEFIYNAIAPAEVDMVLFDDEDTKRATVVVPDSKLSLA
                     IGRRGQNVRLAAHLTGYRIDIKSASEYEKMEAQELQTEEVAQESEVISDKAE"
     gene            5461..5757
                     /locus_tag="VBE34_000650"
     CDS             5461..5757
                     /locus_tag="VBE34_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEO7263389.1"
                     /translation="MAKTKKIPLRKSVVSGEVIDKRDLLRIVKNKEGQVFIDPTGKQN
                     GRGAYIKLDNDEAILAKKKRVFDRSFSMEVSDEFYDELLAYVDHKVKRRELGLE"
     gene            5750..6052
                     /locus_tag="VBE34_000651"
     CDS             5750..6052
                     /locus_tag="VBE34_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEO7263390.1"
                     /translation="MNNSEKVLNLIGLAQRAGRLITGEELVIKAIQNQQVSLIFLAND
                     AGPNLTKKVTDKSNYYKTEVSTVFSTLELSDALGKPRKVVAVADAGFSKKMRTLMA"
     gene            6072..8855
                     /gene="infB"
                     /locus_tag="VBE34_000652"
     CDS             6072..8855
                     /gene="infB"
                     /locus_tag="VBE34_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEO7263391.1"
                     /translation="MSKKRLHEIAKEIGKTSKEVVEQAQSLGLPVKSHASSVEENDAT
                     RIVESFSSSKTKAPTNSVQTNQGVKTESKTVETKQGLSDDKPSTQPVAKPKPQSRNFK
                     AEREARAKAEAEKRQHNGDHRKNNRHNDTRSDDRRHQGQKRSNGNRNDNRQGQQNNRN
                     KNDGRYADYKQKPQTRPQQPAGNRIDFKARAAALKAEQNAEYSRHSEQRFREEQEAKR
                     QAAKEQELAKAAALKAQEEAQKAKEKLASKPVAKVKEIVNKVAATPSQTADSRRKKQT
                     RSDKSRQFSNENEDGQKQTKNKKNWNNQNQVRNQRNSNWNHNKKNKKGKTNGAPKPVT
                     ERKFHELPKEFEYTEGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGDTIELL
                     MVDYGIEAHAKVEVDEADIERFFADEDYLNPDNLTERPPVVTIMGHVDHGKTTLLDTL
                     RNSRVATGEAGGITQHIGAYQIEEAGKKITFLDTPGHAAFTSMRARGASVTDITILIV
                     AADDGVMPQTVEAINHSKAAGVPIIVAINKIDKPGANPERVISELAEHGVISTAWGGE
                     SEFVEISAKFGKNIQELLETVLLVAEMEELKADADVRAIGTVIEARLDKGKGAVATLL
                     VQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGDHFAV
                     YADEKAARAAGEERAKRALLKQRQNTQRVSLENLFDTLKAGEVKSVNVIIKADVQGSV
                     EALAASLLKIDVEGVKVNVVHSAVGAINESDVTLAEASNAVIIGFNVRPTPQARQQAD
                     ADDVEIRQHSIIYKVIEEVEEAMKGKLDPEYQEKILGEAIIRETFKVSKVGTIGGFMV
                     INGKVTRDSSVRVIRDGVVIFDGKLASLKHYKDDVKEVGNAQEGGLMIENYNDLKEDD
                     TIEAYIMEEIKRK"
     gene            8945..9313
                     /gene="rbfA"
                     /locus_tag="VBE34_000653"
     CDS             8945..9313
                     /gene="rbfA"
                     /locus_tag="VBE34_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEO7263392.1"
                     /translation="MKKGLIMANHRIDRVGMEIKREVNEILRLRVNDPRVQDVTITDV
                     QMLGDLSMAKVFYTIHSTLASDNQKAQIGLEKATGTIKRELGKNLTMYKIPDLQFVKD
                     ESIEYGNKIDEMLRNLDKKD"
     gene            complement(9385..10389)
                     /locus_tag="VBE34_000654"
     CDS             complement(9385..10389)
                     /locus_tag="VBE34_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7263393.1"
                     /translation="MKPSFKKLLLLFSIITILSIACTPHAKASGRSWKSWFIEQYFWL
                     KRDKSYYKVQDESSFQKYLNASREQSDKGYYLDPNSVNGGLVQERLFDMQVYSWNDNG
                     KANQKTIFYLAGGSYLNNPTPYHISMLKTLSTSLDAKIILPIYPKTPRYTYDYAIPRL
                     VNLYRHFHEKNANLTLMGDSAGGGLALGLAHALSHQSGQEAIPQPKNIILLSPWLDVT
                     MKHPEIPKYEDTDPILSAWGLARVGEIWANGSNNTNHTYVSPKNAPATKLAPITLFTG
                     TREIFFPDIRDYAAQLQAANHPVNYIAQEGMNHVYPIYPIEEAKTAQYQMIDIINKTP
                     "
     gene            10552..10968
                     /locus_tag="VBE34_000655"
     CDS             10552..10968
                     /locus_tag="VBE34_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEO7263394.1"
                     /translation="MTISNAEWEIMRVVWAQQNTTSNEILAVLLEKYDWTPSTVKTLL
                     RRLLDKGYVSREKMGKGFSYSSLIDEDLAMMSEVDSVLQKVCQTKHVAIVRHLLESIP
                     MTEKDRLNLQASLEAKKGKTLERVACNCIPGQCQCH"
     gene            10981..13215
                     /locus_tag="VBE34_000656"
     CDS             10981..13215
                     /locus_tag="VBE34_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEO7263395.1"
                     /translation="MANRKETFLIDGMTCASCALTIEKAVNKLDHVDSAVVNLATEKM
                     TVTFDDTTLSPNVIEECVSETGYEASLFKEETSKSQSERHQLAIEKMWHRFWMSAVAT
                     IPLLYISMGPMINLWLPSFLMPDKGPLNYGVIQLLLTLPVMYFGRIFYQNGFKALFKR
                     HPNMDSLVAIATTAAFIYSLYGLYEILQGDIHYAHQLYFESVAVILTLITLGKYFEIL
                     SKGRTSASIEKLLTLSAKEARVIKDGEDYMVPLDKVKIGETILVKPGEKIPLDGHVVA
                     GESSIDESMLTGESIPVEKKVGSKVYGASINGQGSLTIFVEKEAGGSLLSQIINLVEV
                     AQASKAPIANLADKVSGVFVPFVIVIAILSGLSWYLILGQSFAFSLKIMIAVLVIACP
                     CALGLATPTAIMVASGKAAENGILFKGGEVLEKAHHIDTIVFDKTGTLTKGKPEVVAI
                     KTYGVDKEEFLGQVASVEKLSNHPLSQTIVNKAKEKELPLREVMAFKNILGYGLSAII
                     NGKTMLVGNANLMTKNDVNLDLAKADIEIAQEEAQTVVYVSENGVLSGLITLTDQLKT
                     DSQETVKQLQRLGFNLVLLTGDNKASADAIAQKLGITTVVSEVLPDQKANVILELKEK
                     GGQIAMVGDGINDAPALASSDVGISMSSGTDIAIESADIVLMKPELTDLLKAMTISKQ
                     TIQIIKENLFWAFFYNVLAIPVAMGVLHLFGGPLLNPMLAGLAMAFSSVSVVLNALRL
                     KVLK"
     gene            13256..13462
                     /locus_tag="VBE34_000657"
     CDS             13256..13462
                     /locus_tag="VBE34_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEO7263396.1"
                     /translation="MKHTYRVSGMKCDGCAKTVSDKLSSVMGVDEVNVDLTKNQVVVS
                     GKTFKCLLKRSLKDTKYSLEEEIN"
     gene            13572..14186
                     /locus_tag="VBE34_000658"
     CDS             13572..14186
                     /locus_tag="VBE34_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEO7263397.1"
                     /translation="MSIDIWNILSLVGTVAFASSGAIVAIEEEFDVLGLFILGFVTAF
                     GGGAIRNVLIGLPIETLWSQGIAFYAAAAAILFIMIFPNLLSGKGRDAEVVSDAIGLA
                     AFSVQGALYATQSHQPLSAVIVAAVLTGAGGGIVRDVLAGRKPGVLRSEIYAGWSILV
                     GIILYFKIAKTTTDYYLLVLVVTSLRMLGYKKQWHLPVVRPRKK"
     gene            14201..15013
                     /locus_tag="VBE34_000659"
     CDS             14201..15013
                     /locus_tag="VBE34_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7263398.1"
                     /translation="MIKLIAIDMDGTLLNDEKKIPKENIQAIKEATQAGIKIVLCTGR
                     PMSGILPYFNELGLTKEEYIIMNNGCSTYSTKDWQLIDSATLTHDELIFLEEVVKEFP
                     NVCLTLTAENTFYAVGEEVPEIVAYDADLVFTKAKSISLDALRNQEEIVFQAMYMGLD
                     ADVTAFQEAVEEALISKFSGVRSQDYIYEIMPQGVTKARGLKSLIAKLGLDINQVMAI
                     GDAPNDIELLDLVPNSVAMGNASDEIKSRCKYITVDNNQAGVAKAIYDYALR"
     gene            15126..17768
                     /gene="polA"
                     /locus_tag="VBE34_000660"
     CDS             15126..17768
                     /gene="polA"
                     /locus_tag="VBE34_000660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEO7263399.1"
                     /translation="MTNKNKLLLIDGSSVAFRAFFALYNQIDRFKNNSGLHTNAIYGF
                     HLMLNHILGRVQPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPYIRQQLD
                     VLGIKHYELEHYEADDIIGTLAKQAEASKEHFDITVVSGDKDLIQLTDTNTVVEISKK
                     GVAEFEEFTPAYLMEKMGITPSQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLSEYGS
                     LEGIYENIEAMKQSKMKENLINDKEQAFLSKTLATINIASPITIGLEDILYSGPQDIK
                     ALSQFYDEMDFKQFKAALGEETSQEDFEVDFTEVEQLKPEMFSDNDFYYFEMLGDNYH
                     VEDLIGIAWGNSDTIYATSDVSLLQEALFKKALSKPIKTYDFKRSKVLLNRFNIDLPE
                     PAFDTRLAKYLLSTTEDNLVSTIARLYTNLPLDTDDAVYGKGAKRAIPEKTRFLEHLA
                     KKVKVLVDSEANVMQQLKANEQEELLFEMEQPLANVLAKMEIRGIKVKKNTLNEMAIE
                     NQKVIETLTQEIYELAGQEFNINSPKQLGKLLFETLGLPVEMTKKTKTGYSTAVDVLE
                     RLAPISPLVTKILEYRQITKLQSTYIIGLQDYILEDGKIHTRYVQDLTQTGRLSSSDP
                     NLQNIPVRLEQGRLIRKAFVPSEDNAVLLSSDYSQIELRVLAHISKDEHLIAAFKEGA
                     DIHTSTAMRVFGIEKPENVTPNDRRNAKAVNFGIVYGISDFGLSHNLGIPRKLAKQYI
                     DTYFERYPGIKNYMETVVREAKDKGYVETLFHRRRSLPDINSRNFNIRQFAERTAINS
                     PIQGSAADILKIAMINLDRVLDKGGYKSKMLLQVHDEIVLEVPNEEIGAIKELVTKTM
                     ESAISLSVPLIADENAGETWYEAK"
     gene            17798..18238
                     /locus_tag="VBE34_000661"
     CDS             17798..18238
                     /locus_tag="VBE34_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEO7263400.1"
                     /translation="MVYHFQNLSDFMLKNYLTKAKTIAVVGLSDRQETAAYQVSKIMQ
                     EAGYQIIPVNPKNAGQKILGQMTYASLKDVTEHIDIVNIFRRSEYLPDIAREFLEVDA
                     DIFWAQLGLESQEAETILKQAGHKQIVMNKCLKVEYQKLHSETN"
     gene            18320..18799
                     /gene="perR"
                     /locus_tag="VBE34_000662"
     CDS             18320..18799
                     /gene="perR"
                     /locus_tag="VBE34_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEO7263401.1"
                     /translation="MDSHSHGHRPLDAYENVLEHLREKRIRITETRKAIISYMVNSRE
                     HPSAEKIYNDLLPEYPNMSLATVYNNLKVLVDEGFVTELKLCNYSTTYYDFMGHQHLN
                     IACEDCGKIVDFVDVDLLDISREAHQQTGFEVTRVQLVAYGICPECQRKRKDKYKKD"
     gene            18952..20517
                     /locus_tag="VBE34_000663"
     CDS             18952..20517
                     /locus_tag="VBE34_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEO7263402.1"
                     /translation="MKISQYNKWSIRRLKVGAASVMIASGSIVALGQSHIVSADEMSQ
                     PKTTITAPTANTSTNVESSTDKALSKVTTMETSSEMPKMQNMAKVEKTSDKPMMVATS
                     VRKMMATPTPVAMTKTTSVDEVKKSTDTAFKQTVDVPAHYVNAAKGNGPFLAGVNQTI
                     PYEAFGGDGMLTRLILKASEGAKWSDNGVDKNSPLLPLKGLTKGKYFYQVSLNGNTTG
                     KEGQALLDQIKANDKHSYQATIRVYGAKDGKVDLKNMISQKMVTINIPHITTDMEVKN
                     SLKMAFKEKVDVPAKYVSAAKAKGPFLAGVNETIPYEAFGGDGMLTRLILKASEGAKW
                     SDNGVDKNSPLLPLKDLTKGKYFYQVSLNGNTAGKKGQALLDQIKANGSHTYQATITI
                     YGTKDGKVDMNTILGQKTVMIHINVAKKDMNSTSMMMKKDKMTMPMKKEMTSSKINTG
                     MMMSNNKMSANMQMSSQAKSNDKAGKKMSMMSKNLPNTGETKQQNVGVLGMLSLAFAT
                     GLTALGLKKSKQR"
     gene            20630..21316
                     /locus_tag="VBE34_000664"
     CDS             20630..21316
                     /locus_tag="VBE34_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7263403.1"
                     /translation="MTQKLLLVDDEFEIIDINRRYLEQAGYEVSVAADGIEALKEVDE
                     NRFDLIISDIMMPKMDGYDFISEVLVREPNQPFLFITAKVSEPDKIYSLSMGADDFIS
                     KPFSPRELVLRVKNILRRIYGNHQQSEVLTIGDLVIDQKQRLVMVDCNTISLTNKSFD
                     LLWILANHLNRVFSKTELYERVWGEEFLDDTNTLNVHIHALRNDLAKFSTDNTPTIKT
                     VWGLGYKLEE"
     gene            21318..22355
                     /locus_tag="VBE34_000665"
     CDS             21318..22355
                     /locus_tag="VBE34_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7263404.1"
                     /translation="MKLKYYIVIGYLISMLITVAGVFFGLNHMLIETRGVYYILSVTI
                     IACIVGGIVNLFLLSSVFTSLKKLKQKMKDISQRRFDTKAQICSPQEFKDLETAFNQM
                     SSELESTFKSLNESEREKTMMIAQLSHDIKTPITSIQSTVEGILDGIISEEEVNYYLN
                     TISRQTNRLNHLVEELSFITLETMSDTAEPHKEETIYLDKLLIDILSEFQLVFEKENR
                     QVMIDVAPDVSKLSSQYDKLSRILLNLISNAVKYSDPGSPLTIKAYSNRQDIVIDIID
                     QGYGIKDEDLASIFNRFYRVESSRNMKTGGHGLGLYIARQLAHQLNGDILVESQYQKG
                     SKFSLVLKLQK"
     gene            complement(22369..23109)
                     /locus_tag="VBE34_000666"
     CDS             complement(22369..23109)
                     /locus_tag="VBE34_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEO7263405.1"
                     /translation="MTNSEIKNEAKTILSNLQGKNQLFLLPILLSIITLYISFYYQYN
                     NMTLLGFFVPLPVYFFYTLFIISVSFVMLDVVKNQKLNVRFSDNTYVFSSHIFWKLLS
                     VLVLKGLILSFFYLLSTFGLLIIISSFRLLLSGNLILAPVLIVVSSLITTKAVIKLVQ
                     QYYSYSISTLVFYTQLESGNYEGPSKVLVASRELMNGNKLRLFLLDLSFIGWHFLTIF
                     SFGLVYIYLLPYQTTARLIFYRNITKNS"
     gene            23296..24438
                     /gene="tgt"
                     /locus_tag="VBE34_000667"
     CDS             23296..24438
                     /gene="tgt"
                     /locus_tag="VBE34_000667"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEO7263406.1"
                     /translation="MTDHPIKYRLIKQEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIAKAGGLHKFMNWDQAILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGAKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLNAHRRPHDQGLFGIVQGAGFEDLRRQSARDLVSMDFPGYSI
                     GGLAVGETHDEMNAVLDFTVPMLPNDKPRYLMGVGAPDSLIDAVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKFAEDFTPLDPNCDCYTCKNYTRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKDVRQAIMDDNLLEFRQDFMERYGYGTNNRNF"
     gene            24544..24855
                     /locus_tag="VBE34_000668"
     CDS             24544..24855
                     /locus_tag="VBE34_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263407.1"
                     /translation="MMQEYFGIGLDSSSRCYHYHTKLDIVALKCAVCQKYYACYKCHD
                     ALEEHCFAATKSDEMFPVLCGSCRQMLTLKEYKTGFCPYCRMLFNPNCQRHDSIYFSK
                     E"
     gene            24862..25401
                     /locus_tag="VBE34_000669"
     CDS             24862..25401
                     /locus_tag="VBE34_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEO7263408.1"
                     /translation="MFHTKSLIQMAMMATLLVVLGFIPALPLGFIPVPIVLQNLGVMM
                     AGVILGGRKGSLSILLFFLVGLVIPVFSGLRSTIPVLTGPTAGYVIAWLFVPLLISYG
                     VKLVNKKNFIAIFLIVWLAGVLFVDVAGAVWLASYMNIPLDKSLLQNLVFLPGDTIKA
                     IIAAVVAVKYKDSFFLTEK"
     gene            25540..26316
                     /locus_tag="VBE34_000670"
     CDS             25540..26316
                     /locus_tag="VBE34_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO7263409.1"
                     /translation="MKLTTLGCWGGYPYKDGGTTSFLLTGENNFHLLIDAGSRSINEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLYHYRLLYPKDKWEPKVLPVYGHNEDDYEFS
                     KLTLPNVAQGIAYHTNGVEQVGPFNITFIKTVHPVVCYAMRITERETGQVLVFTGDSG
                     YFADLVPFTKGADLFLADVYLYEGNENHIAHLTSKEAGEIAYNAKVKKLVLTHVPQFP
                     GEGINPENHLEELRLQSEKYAGGISVDLAQPHKSWDLGKL"
     gene            26316..26822
                     /locus_tag="VBE34_000671"
     CDS             26316..26822
                     /locus_tag="VBE34_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEO7263410.1"
                     /translation="MQKFTQEEKEWFMTEALKEAKSSLEKEEIPIGCVIVKDGHIIGR
                     GHNAREEFNKAILHAEIMAINNANEKVGNWRLLDTTLFVTVEPCVMCSGAIGLARIPH
                     VIYGAKNTKFGAAGSLYDILSDSRLNHRVEVETGILEDQCAKIMQDFFRKGRQRKKEA
                     KLSEKTKI"
     gene            26857..26942
                     /gene="ffs"
                     /locus_tag="VBE34_000672"
     ncRNA           26857..26942
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VBE34_000672"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            27094..28443
                     /locus_tag="VBE34_000673"
     CDS             27094..28443
                     /locus_tag="VBE34_000673"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEO7263411.1"
                     /translation="MTHITFDYSKVLGQFVGEHELDYLQPQVSAADAFLRQGTGPGSD
                     FLGWMDLPENYDKEEFSRIQKAAEKIKSDSEVLVVIGIGGSYLGAKAAIDFLNNHFAN
                     LQTAEERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVVRVEK
                     PRKNVTIPELTEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPTQDAY
                     TLGYTIYFFELAIGLSGYLNSVNPFDQPGVEAYKRNMFALLGKPGFEELSAELNARL"
     gene            28765..29292
                     /locus_tag="VBE34_000674"
     CDS             28765..29292
                     /locus_tag="VBE34_000674"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEO7263412.1"
                     /translation="MEKKLLRKEVLITLKSQPQAYKSEVDSKLLEAFIKTKAYQNSCV
                     IATYLSFDYEYNTQLLIKQALCDGKRVLVPKTYPKGKMIFVDYQKDNLRATPFGLLEP
                     VNDRAVEKASIDLIHVPGLIFNNKGFRIGYGAGYFDRYLSDFEGDTISTIYRCQRQDF
                     VEEKHDVAVKEVLCL"
     gene            29283..29960
                     /locus_tag="VBE34_000675"
     CDS             29283..29960
                     /locus_tag="VBE34_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEO7263413.1"
                     /translation="MSMKKFAKEYPTTVLLVGLTTLVFLLMQLTYGSQAESSQVIFQF
                     GGIQGDYLKAYPTNLWRLISPIFVHIGWEHFLLNGLALYFVGQMGESIWGSLRFLILY
                     ILSGLMGNIFTLFFTPHVVAAGASTSLFGVFSAIAIAGYFGKNPYLKQVGKSYQVMIL
                     LNLFFNIFTPGVSLAGHVGGLVGGVLVAIFLTKQNGSLLFKTWQSILALMIFIIVSIS
                     LIGLSLV"
     gene            30092..31135
                     /locus_tag="VBE34_000676"
     CDS             30092..31135
                     /locus_tag="VBE34_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO7263414.1"
                     /translation="MNRKIIGLTLLSLSVLTLTACGNRSDKSANKSDIKVAMVTNQGG
                     VDDKSFNQSAWEGLQKWGKKKGLTKGNGFDYFQSSNESDHANNLDTAASSGYNLIFGI
                     GFGIHDTIEKVSENNKDVKYVIVDDIIKGKENVASVTFADNEAAYLAGVAAAKTTKTK
                     TVGFIGGMEGVVVKRFEAGFKAGVKSIDPAIKVAVSYAGSFTDAAKGKTIAATQYATG
                     VDVIYQAAGGTGAGIFSEAKTENETRKESNKVWVIGVDRDQSQEGNYVSKDGKKANFV
                     LASTIKEVGKSLQSVAELTEKKQYPGGKVTVFGLKDSGVDIKEHQLSSEGSVAVKKAK
                     EGIVSGKIQVPMK"
     gene            complement(31226..32125)
                     /gene="galU"
                     /locus_tag="VBE34_000677"
     CDS             complement(31226..32125)
                     /gene="galU"
                     /locus_tag="VBE34_000677"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEO7263415.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKNELLKLVDETTGIRLHFIRQ
                     SHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNNKVIPLTKQLINDFEATHASTIA
                     VMEVPHEDVSAYGVIAPQGEGVNGLYSVNTFVEKPSPEEAPSNLAIIGRYLLTPEIFN
                     ILETQKPGAGNEIQLTDAIDTLNKTQRVFARKFTGDRYDVGDKFGFMKTSIDYALQHP
                     QVKDDLKKYIIDLGKSLEKTSKK"
     gene            complement(32162..33178)
                     /locus_tag="VBE34_000678"
     CDS             complement(32162..33178)
                     /locus_tag="VBE34_000678"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO7263416.1"
                     /translation="MTKQKIAVLGPGSWGTALAQVLNDNGHEVRLWGNVVEQIEEINT
                     NHTNQRYFKDITLDSKIKAYTNLEEAINNVDSILFVVPTKVTRLVAKQVASLLKHKVV
                     IMHASKGLEPGTHERLSTILEEEISEQYRSDIVVVSGPSHAEEAIVRDITLITAASKD
                     IEAAKYVQKLFSNHYFRLYTNTDVVGVETAGALKNIIAVGAGALHGLGYGDNAKAAII
                     TRGLAEITRLGVQLGADPLTFSGLSGVGDLIVTGTSVHSRNWRAGDALGRGEKLEDIE
                     KNMGMVIEGISTTKVAYEIAQNLNVYMPITEAIYKSIYEGANIKDSILDMMSNEFRSE
                     NEWH"
     gene            33348..33677
                     /gene="rnpA"
                     /locus_tag="VBE34_000679"
     CDS             33348..33677
                     /gene="rnpA"
                     /locus_tag="VBE34_000679"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEO7263417.1"
                     /translation="MKKTYRVKSDKDFQMIFSRGKNVANRKFVIYYLEKEQKHFRVGI
                     SVSKKLGNAVVRNAVKRKIRHVLLSQKTALQDYDFVVIARKGVEELDYQALEKNLIHV
                     LKIAGLI"
     gene            33690..34505
                     /locus_tag="VBE34_000680"
     CDS             33690..34505
                     /locus_tag="VBE34_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO7263418.1"
                     /translation="MKKKLKTFSLILLTGSLLVACGRGEVSSHSATLWEQIVYAFAKS
                     IQWLSFNHSIGLGIILFTLIIRAIMMPLYNMQMKSSQKMQEIQPRLKELQKKYPGKDP
                     DSRLKLNDEMQSMYKAEGVNPYASVLPLLIQLPVLWALFQALTRVSFLKVGTFLSLEL
                     SQPDPYYILPVLAALFTFLSTWLTNKAAVEKNIALTLMTYVMPFIILVTSFNFASGVV
                     LYWTVSNAFQVFQILLLNNPYKIIKVREEAVRVAHEKEQRVKRAKRKASKKRK"
     gene            34513..35334
                     /locus_tag="VBE34_000681"
     CDS             34513..35334
                     /locus_tag="VBE34_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEO7263419.1"
                     /translation="MVLFTGATVEEAIEKGLQELNISRLRAHIKVVSREKKGFLGFGK
                     KPAKVEIEGITDEVTDINESVALKNIKNVPSSVDVVEEYIEEVDETLEKEDVSQPELP
                     KIDDKNVVTTSEAIEKIDLLPNIEVAAAQVTKYVENIIYEMDLDATIETTTSKRQINL
                     QIETPEAGRIIGYHGKVLKSLQLLAQNYLHDRFSKSFSVSINVHDYVEHRIETLIDFS
                     KKIARRVLETNEPYHMDPMSNSERKTVHKTIATIEGVESYSEGNDPNRFVVVTKK"
BASE COUNT        11913 a         5429 c         7053 g        10961 t
ORIGIN      
        1 tataatagtt aataaagtat atcaaagata gagagaatga caatgttaaa aattgaaaat
       61 gttacagggg ggtatgttaa tatcccagtt ttaaaaaata tttcttttga agtaaatgat
      121 ggagagttag ttggattaat tgggcttaat ggtgcaggaa aatcaaccac tattaacgag
      181 attattggta ttttgagacc ctatcaagga gatattacca ttgatggcat tagtcttgaa
      241 gccaaccaag aactttatag aaagaaaata ggctttattc ctgagacacc aagtctctat
      301 gaggaactaa ctctcagaga acatttagaa acggtagcta tggcgtatga tattgccacc
      361 gatgaagtta tggcacgtgc gcaaaaacta ttagagatgt ttcgtctaac agataaatta
      421 gactggtttc caatgcattt ttcaaaggga atgaaacaaa aggttatgat tatctgtgct
      481 tttgttgtat ctccaagttt atttattgtt gatgaaccat ttttaggttt ggatccacta
      541 gctatttcag atttgattaa tcttctagca gaagaaaaag ctaaagggaa atctattttg
      601 atgtcaacac acgtattaga ttctgccgaa aaaatgtgtg atcgttttgt tattttacat
      661 aaaggagaaa tacgggcagt tggaacccta gaggaattac gggctatttt tggagacagt
      721 aatgctaatt taaatgacat ctacatagct ctgacaaaag aggtgtgata tgaaaaagtt
      781 atttaataaa cgtcgaagtc tctttttaac tcaaaatagt aaatatttga gatacgtctt
      841 taatgatcat tttgtattag tattaatgtt tttgtcaggt tttttgttat atcagtatag
      901 ccagttacta aaagactttc caaaaacaca ttggccaatt atcgtgattg tgtcaatcat
      961 tatcttaatg ttactagcga tgggggaaat agccagctat ttagaaccgg ctgataaaca
     1021 atttttactt ataaaagaag aagcaataaa ggaaattata aattctgcta aaaaacggac
     1081 ttatatcttt tggttggttg ttcaaacctt atttttagta ttaatatcac caattttgat
     1141 aaagttaggc ttgtcagttt ttatgattac attgttaatt tttggtctag gaataatcaa
     1201 atggttggtg ataacttaca aggtaaaggt cttttataac aatcaaaatt tgaactggga
     1261 tgcagctata aatcatgagc aggaaagaaa acaggctata ttaaaatttt tctctttgtt
     1321 tacaaatgtt aagggaattt cgacaagtgt aaaacgtcgt tcatttttag atgttatttt
     1381 aaaacttatt tctaaaacgc catcgcgttt atggactaac ttatttgtga gggcatttct
     1441 aagaagctca gattatcttg gtttaaccat aaggttggta actttaaata tcttatcagt
     1501 catctttgtt aacgaaacct acctcgccct tgctctggca tttgtattta actacttatt
     1561 attgttccaa ttattagctt taggtcacca ttttgattat caatacatga atcagctcta
     1621 tccagttaga ttaaatgcta aagcaagtca gctaaaaggt tttctcagag tgttatcata
     1681 tgctgtgaca gtaattgata tcatacttat aagagaattg aaacctgtta tattattgat
     1741 agttttgatg ttaattgtga cggaatatta catcccttac aaaataaaga agatgattga
     1801 ctaaagccta taaaacaagt aaaataagag gaaacgacta aatgaaaagg agggactgct
     1861 ggtgacaata tcaaatcaag aattgacgct tactccctta agaggaaaaa gtggcaaagc
     1921 ctatattggt acttatccaa atggtgaacg tgtctttgtc aaatataata cgacacctat
     1981 tttgccagcc ttagctaagg aacagattgc gccacaacta ctgtgggcac gccgtacaag
     2041 taatggcgat atgatgagtg ctcaagaatg gttagatggt cgaacattga ctaaagaaga
     2101 tatgggtagt aaacagatta ttcatatttt actgcgcctg cacaagtctc gtcccttagt
     2161 aaatcaactg ttacaattag ggtataaaat tgaaaaccct tatgatttgt tgatggattg
     2221 ggaaaaacaa actcctattc aaatccgaga aaacacttat ttgcaatcta ttgtgactga
     2281 gttaaagcgt agtttacctg agtttcgaac agaggttgca acaatcgttc acggagatat
     2341 caaacatagc aactgggtta ttacaacaag tggtttaatt tatttggttg attgggattc
     2401 tgttcgttta acagatcgca tgtatgacgt tgcatatatt ttaagtcatt atattccaca
     2461 aaaacactgg aaagattggc tgtcttacta cggttataaa gataatgaga aagtctggag
     2521 caaaattatt tggtatgggc aattttcata tttatcacag ataatcaaat gttttgataa
     2581 acgagatatg gagcatgtca atcaagaaat ttatgaatta cgaaagttta gagagttaat
     2641 taaaaagtat aatgcgagtt agaaaacgta agggtgcaga ggaacactta gaaaataatc
     2701 cgcactatgt tatttcaaat ccagaagaag ccaaagggcg ttggcatgag atttttggaa
     2761 ataataaccc cattcacata gaagttggtt ccggaaaagg ggcttttatc actggtatgg
     2821 cagagcaaaa tccagatatc aattatattg gaattgatat tcaattgtca gtgctttcct
     2881 atgctctgga taaagttcta gatagcggtg ctaagaatat caaattacta ttagttgatg
     2941 gttctagtct atcaaactac tttgatactg gcgaagttga tttgatgtat cttaacttta
     3001 gtgatccatg gcctaagaaa aagcatgaga aacgtcgatt gacctataag actttcttgg
     3061 atacctataa agacatactt cctgagcagg gggaaattca ttttaaaaca gataatagag
     3121 gtttatttga atatagcctt gctagctttt ctcaatacgg tatgacttta aaacaagttt
     3181 ggttagattt acatgcaagt gattaccagc aaaatatcat gactgagtac gaacgcaaat
     3241 tttcaaataa aggacaggtc atctatcgtg tagaagcacg attttagaat aagtaaatag
     3301 taagtgggta gttatttgct actcctttat ggagaggtcg catagtggct gagtgcgcac
     3361 gcttggaaag cgtgtagtcc ctaatggggg ctcgggggtt cgaatcccct cctctccttt
     3421 aatcttgtta atgtatcagt gtttattaag tgttcacctt tttcactcgt caaaagtcca
     3481 agtttcgaaa aatttctttt attttatcat cgtttctctt tgagcttgaa gtattagcat
     3541 aaagttttct aagctcttac aaagaattga ggacaaattt taattttttg atataagatt
     3601 agtgtacggt gatgctaatt taaagtaaat gattgcaata atcgtatttt gtgctataat
     3661 agtattaaat aatacagaaa ggggcggagt tttacttcgc ctcttatgtg tgtgattcat
     3721 agaatttata gtcattgtaa gttctaaagc aagttagaaa gaagaggtag ctgttatcgc
     3781 aaatcaaacc attgttgata ttgttactca agtcgtaact cctgctattc aagcaccgtt
     3841 tgaattagta gatgtagaat acgaaaaaat gggtggcgac tatgtgctca gtattttaat
     3901 tgataaaccc ggaggcatta cggttgaaga tacagcacaa ttgacagacg ttgtaagtcc
     3961 cttacttgac acaattcaac cagatccatt tccagaacag tatatgcttg aagtctcaag
     4021 tcctgggttg gaaaggccac ttaaaacagc agaagcatta tcaaatgctg ttgggtctta
     4081 tatcaatgta agtctgtata aatccattga taaagttaaa atatttgaag gtgacttatt
     4141 atcatttgat ggtgaaacct tgacaattga ttatatggac aaaacacgtc ataaaactgt
     4201 agatattccc tatcagacgg ttgcaaaagc ccgtcttgcg gttaaacttt aagaaaggat
     4261 gattcttgcg aagcaagaat ggacactatg agcaaagaaa tgctagaagc cttccgtatt
     4321 ttggaagaag aaaaacacat taataaagaa gatattattg atgcagttac tgagtcacta
     4381 aaatctgcct ataagcgtcg ttatggtcaa tcagaatcat gcgttattga atttaacgag
     4441 aagaaagcag actttaccgt ctatactgtt cgtgaagttg ttgatgaagt ttttgatagc
     4501 cgtttggaaa ttagcctaaa agacgcactt gctattagct cagcgtatga acttggtgat
     4561 aagattcgct ttgaggaatc tgttacagaa tttggacgcg tggcagctca gtcagcaaaa
     4621 caaactatta tggaaaaaat gcgtcgtcaa atgcgtgaag taaccttcaa tgaatacaaa
     4681 caacacgaag gtgaaattat gactggtact gttgaacgat ttgaccaacg ttttatctat
     4741 gtcaatctcg gttcactcga ggcgcaatta tcgcatcaag atcagattcc tggggagagc
     4801 tttaaatctc acgatatgat tgacgtttat gtctataaag tggagaataa tcctaaaggt
     4861 gtcaacgttt ttgttagccg tagtcatcca gagtttatca aacgcattat ggagcgagaa
     4921 attccagaag tatttgatgg aactgttgag attatgagcg tctctcgtga ggctggagat
     4981 cgtactaaag ttgctgtacg cagtcataat tcaaacgttg atgcgattgg aacaattgtt
     5041 ggacgcggtg gaagtaacat taaaaaagtt atcagtaatt tccatcctaa acgcgttgat
     5101 gctaaaacag gattggagat cccagttgaa gaaaacattg atgttattca atgggttgaa
     5161 gatccagcgg aattcattta caatgctatt gcaccagccg aagttgatat ggtacttttt
     5221 gatgatgaag atacaaaacg tgctactgta gttgttccag atagcaagtt atcgctagct
     5281 attggtcgcc gaggacaaaa tgtgcgttta gcagctcatt tgactggcta tcgtattgat
     5341 attaaatctg caagtgaata cgaaaaaatg gaagcgcaag aacttcaaac tgaggaagta
     5401 gctcaagaat ctgaagttat ttcagataag gcagaataat ataaatgaga gaggtgtttt
     5461 atggctaaaa caaaaaaaat acctttaaga aaatcagttg tttctggtga agtgatcgat
     5521 aagcgagatc ttttacgaat tgttaaaaat aaagaaggac aggtttttat agatcctacc
     5581 ggaaagcaaa atggtcgagg tgcctacata aaattggaca atgatgaagc tatcctagcg
     5641 aaaaagaaac gagtgtttga tcgcagtttt tcaatggaag tttctgatga attctatgat
     5701 gaactattgg catatgtcga ccataaggtc aaaagaagag agttaggcct tgaataattc
     5761 agaaaaagta ttaaatctca ttgggttagc acagcgtgcg gggcgcctta ttacaggtga
     5821 agaattggtt attaaagcaa ttcaaaatca acaagtatcg ctaatctttc tagcaaatga
     5881 tgctggccca aatttgacga aaaaagtaac tgataaaagt aattactaca aaacagaagt
     5941 ctccacagtg ttttcaacac tggaattaag tgatgcgtta ggcaaaccaa gaaaagtggt
     6001 tgctgtcgca gacgctggat tttcaaagaa aatgaggact cttatggcat aacagaatag
     6061 gaggacatca attgtcaaag aaaagattac acgaaatcgc taaggaaatt ggaaaaacga
     6121 gtaaggaagt ggttgagcaa gctcaatcgc ttggcttacc tgtaaaaagt catgcttcta
     6181 gcgttgaaga aaatgatgct acacgtattg ttgagagttt tagctcttct aaaactaaag
     6241 ctccaacaaa ttctgttcaa actaatcaag gtgtgaaaac agaatctaaa actgttgaaa
     6301 ccaaacaagg cttgtccgat gataaacctt caactcaacc tgtagctaaa ccgaaaccac
     6361 aaagtcgtaa ctttaaggcg gagcgcgagg ctcgtgctaa agctgaagcg gagaagcgtc
     6421 aacataatgg ggatcaccgt aagaataatc gccataatga tacacgctct gacgacagac
     6481 gccatcaagg tcaaaagcgt tcaaatggta atcgtaatga taaccgacaa ggtcagcaaa
     6541 ataaccgtaa taaaaatgat gggcgttatg ctgattataa acagaaaccc caaacgcgtc
     6601 ctcagcaacc tgcaggcaat cgcattgact ttaaagctcg agcagcagct ttgaaagctg
     6661 agcaaaatgc tgaatactca cgtcatagtg agcagcgttt ccgtgaagag caagaagcca
     6721 aacgtcaagc agctaaggag caagaactag ctaaagcagc agcgttaaaa gcgcaagaag
     6781 aagctcaaaa agctaaggaa aaacttgctt ctaaacctgt tgctaaggta aaagaaattg
     6841 tcaataaagt agccgctacg ccatcacaga cagcagatag tcgtcgtaaa aaacaaactc
     6901 gttctgataa gtctcgtcaa ttttcaaatg aaaatgaaga tggtcaaaag caaacaaaaa
     6961 ataagaaaaa ctggaataat caaaaccaag tgagaaatca aagaaatagt aactggaatc
     7021 ataataagaa aaataaaaaa ggtaagacta atggagctcc aaaaccagta acagagcgta
     7081 aattccatga attaccaaaa gaatttgaat atactgaggg catgaccgtt gcggaaattg
     7141 caaaacgtat taaacgcgaa ccagcagaaa ttgttaaaaa actctttatg atgggagtta
     7201 tggcaacgca gaatcaatca ttagatggag acactattga gctattgatg gttgactacg
     7261 ggattgaagc acatgctaag gttgaagttg atgaagctga tattgaacgc ttctttgcag
     7321 atgaggatta ccttaatcct gataacttaa cggaacgtcc gcctgttgtt actatcatgg
     7381 gacacgttga ccatggtaaa acaactcttc ttgatactct acgtaattca cgtgtcgcta
     7441 ctggcgaagc tggtggaatt actcaacata ttggtgctta tcagattgaa gaagctggca
     7501 agaaaattac tttcttggat acaccagggc atgcggcctt tacttctatg cgtgcgcgtg
     7561 gtgcatcagt aactgatatt actatcctta tcgttgccgc tgatgatggt gtcatgccgc
     7621 agacagttga ggcaattaat cactctaaag ctgcgggtgt cccaattatt gttgctatca
     7681 acaaaattga taaacctggt gcaaatccag aacgcgttat cagtgaatta gctgagcatg
     7741 gtgtcatctc tactgcatgg ggtggtgagt ctgaatttgt tgaaatttca gctaagtttg
     7801 gtaaaaatat tcaagaatta cttgagacag ttcttttagt tgctgaaatg gaagaattaa
     7861 aagcagatgc agatgttcgt gctattggta ctgttattga agcccgtctt gataaaggta
     7921 agggagccgt tgcaacgctt cttgtccaac aaggtacttt aaatgtacaa gatccaatcg
     7981 ttgttggtaa tacatttgga cgtgttcgtg cgatgactaa tgatcttggg cgtcgtgtca
     8041 aagttgcggg tccatctaca ccagtttcta ttactggttt gaacgaagct ccaatggctg
     8101 gtgatcattt tgctgtttat gcagatgaga aagctgcgcg tgctgcaggt gaagaacgtg
     8161 caaaacgtgc tctcttaaaa caacgccaaa acacgcaacg tgttagcctc gagaacttat
     8221 ttgataccct taaagctggt gaagttaagt cagttaatgt tattatcaaa gctgatgtgc
     8281 aaggttctgt agaagcactt gcagcttctc tccttaagat tgatgttgaa ggagtaaaag
     8341 ttaatgttgt tcactcagca gttggtgcta ttaatgagtc tgatgtaact cttgctgaag
     8401 catcaaatgc tgttattata ggatttaacg tacgccctac tccacaagct cgtcaacaag
     8461 ccgatgctga tgatgtcgaa attcgtcaac atagcattat ctataaagtt atcgaagaag
     8521 ttgaagaagc gatgaaaggt aaacttgatc ctgaatacca agaaaaaatc cttggtgaag
     8581 ccattattcg tgagaccttt aaagtttcaa aagtgggaac tattggtgga tttatggtta
     8641 tcaatggtaa agttactcgt gattcaagtg ttcgtgttat ccgtgatggt gttgtcattt
     8701 ttgatggtaa attagcaagt cttaaacact ataaagatga tgttaaagaa gtcggcaatg
     8761 ctcaagaagg tggtctaatg attgagaatt ataatgatct caaagaagat gacactattg
     8821 aagcatacat tatggaagaa attaaacgta aataacccct atgagaaacc caacttgtat
     8881 ctttagttgg tagggaatac aaaattatgg ctaggctcgt cctagctttc aagttatgag
     8941 cacaatgaag aaaggattaa ttatggctaa tcatcgtatt gatcgcgttg gaatggaaat
     9001 taaacgtgaa gtcaatgaga ttttgcgtct ccgagttaat gatccacgtg tacaagatgt
     9061 cacaattact gatgttcaga tgttgggtga cctttcaatg gctaaagtat tttatactat
     9121 tcattcgaca cttgcttctg ataatcaaaa agcacaaatt ggtttagaaa aagcaactgg
     9181 tactattaag cgtgaacttg gcaaaaacct tactatgtat aaaataccag atttacaatt
     9241 tgttaaagat gaatctattg aatacggaaa taagattgat gaaatgttgc gaaatctaga
     9301 taaaaaagat taagaataat aaaaaataaa ccatgtcaaa atcatggttt attttttata
     9361 gctttagagt tgagatagct agtattaagg agttttattg atgatatcaa tcatttgata
     9421 ctgtgctgtt ttagcttctt caatagggta aattggataa acatgattca ttccctcttg
     9481 tgcaatatag ttaacggggt gattagctgc ttggagttgt gcagcataat cgcgaatatc
     9541 tgggaagaat atttctcgtg ttccagtaaa taacgtaata ggagcgagtt tagtagcagg
     9601 ggcattttta ggactaacat atgtatggtt agtattatta gaaccatttg cccaaatttc
     9661 acctacacgt gcgagacccc aagcagatag gataggatcg gtatcttcgt atttgggaat
     9721 ttctggatgt ttcattgtca catctagcca tggagataag aggataatat ttttaggttg
     9781 aggaatagcc tcttgccctg attggtgaga caatgcatgc gctaatccta gtgctaatcc
     9841 tccgccagct gaatcaccca tcaaagtgag attagcgttt ttttcatgaa agtgtctata
     9901 aaggttaacc aatctaggga tagcataatc ataggtataa cgaggagttt ttggataaat
     9961 tggtaaaata atttttgcat caagactagt tgagagcgtc ttaagcatgc tgatatggta
    10021 aggcgttgga ttatttaagt aagagccacc agctaaatag aaaatggtct tttgattagc
    10081 tttaccgttg tcattccaag aatagacttg catatcaaaa agtctctctt gaacaagccc
    10141 tccattaaca gagtttgggt ctaagtaata acccttatca gattgttcac ggctagcatt
    10201 taagtacttt tgaaatgagc tttcatcttg aactttataa taacttttat cgcgctttag
    10261 ccaaaagtat tgttcgataa accaactttt ccatgaacgc ccacttgctt ttgcatgtgg
    10321 tgtacaggcg atggataaaa tggtgataat actaaatagt agcaatagtt ttttgaagga
    10381 cggtttcaag gtattctcct taatataatg ttatttttgt gtaatattat tataataaaa
    10441 ccaacatctt tgtcaataga ataaagaaag atgagaaatg tttttcggtt tacaaatgta
    10501 gatttatttg ctaaaattgg tttacaaacg taaatcgaaa gaggtgagaa aatgactatt
    10561 tcaaacgcag aatgggaaat tatgagagtg gtttgggcac agcagaatac aaccagtaat
    10621 gagatactag cagttttact agagaagtat gattggactc cttcaactgt caaaacctta
    10681 ttaagaaggc tcttagataa aggctacgtg agcagagaaa aaatgggaaa aggatttagc
    10741 tattcttctt taattgatga agatttagct atgatgtcag aggtagatag tgtattgcag
    10801 aaagtttgcc agaccaaaca tgttgctatt gttagacact tattggaaag tataccaatg
    10861 acagagaagg atcgattaaa tttgcaagca tcactagaag ctaagaaagg caaaacactt
    10921 gaacgtgtag cttgtaattg cattcctggt cagtgtcaat gccattagaa aggagaaaat
    10981 atggctaata gaaaagaaac atttttgatt gatgggatga cttgtgcatc ttgtgcttta
    11041 acgatagaaa aagctgtcaa caagttagac catgtggata gtgcagtagt aaatttagca
    11101 acagagaaaa tgacagttac gtttgatgat acaaccttat cgccaaacgt gattgaagag
    11161 tgtgtttctg agactggata cgaagcatcg cttttcaaag aggaaacttc taaaagccaa
    11221 tctgaacggc accaattagc aattgaaaag atgtggcatc gcttttggat gtcagcagtt
    11281 gcaactatac ctcttctcta tatttccatg gggccaatga ttaacttatg gttaccatca
    11341 tttttgatgc cagataaggg acctctaaac tatggtgtga tacaattatt gttaacacta
    11401 ccggtgatgt attttgggcg tatcttttac caaaatggtt ttaaagcact ttttaaaagg
    11461 catcccaata tggattcatt agttgctata gccacaacag cggctttcat ttatagctta
    11521 tatggattgt acgaaatatt acagggtgac atacactacg ctcaccagct ttatttcgaa
    11581 tctgttgcag ttattttaac acttattact ctaggaaaat attttgaaat attatccaaa
    11641 gggcgtactt cggcctcaat tgaaaaatta ttaactttat cagcaaaaga agcaagagtt
    11701 atcaaagatg gtgaagacta tatggttcct ttggataaag ttaaaatagg tgaaacaatt
    11761 cttgtcaaac ctggagaaaa aattcctctt gatggccatg ttgtagcagg agaatcaagt
    11821 attgatgaat ccatgttaac aggtgaaagt attccagttg aaaaaaaggt tggtagtaag
    11881 gtttacggtg ccagcattaa tgggcaaggt agcttaacga ttttcgttga aaaagaagct
    11941 ggcggttccc ttctatcaca aattattaac ttagtagaag ttgcacaggc aagtaaggct
    12001 ccaattgcta acttagcaga caaagtatca ggagtctttg tcccttttgt tattgtcatt
    12061 gcaattttat caggtttgag ttggtactta attctggggc aaagttttgc cttctctctg
    12121 aagattatga ttgcagtctt agtgattgct tgtccatgtg ctttagggtt agcaactcct
    12181 acagctatta tggtggctag tggtaaagca gctgagaatg gtattttatt taaaggtggt
    12241 gaagtgttgg aaaaggctca ccacattgat acaatagttt ttgataaaac aggaaccttg
    12301 accaaaggga aaccagaagt agttgctatc aaaacatatg gggtagataa agaggagttt
    12361 ttaggtcaag tagcatcagt agaaaaactt tcaaatcatc cacttagcca aacaattgtt
    12421 aacaaagcaa aagagaaaga acttccctta agggaagtca tggcttttaa aaatattttg
    12481 ggttacggtt tgagtgcaat tattaatggt aaaactatgc ttgtaggtaa tgctaactta
    12541 atgactaaga acgatgttaa tttagattta gcaaaggctg atatagaaat agctcaagaa
    12601 gaagcacaaa ctgtggttta cgtttccgaa aatggagtgt tgagcggttt aataacttta
    12661 acggaccagc taaagacaga tagccaagaa acagttaagc agttacaaag attaggattt
    12721 aatttagttt tacttactgg tgataataag gctagtgcag atgccatagc acaaaaattg
    12781 ggtatcacga cagttgtttc agaagtttta cctgatcaga aagcaaacgt tattttagaa
    12841 cttaaagaaa aaggcggaca gattgcgatg gttggtgatg gcatcaatga tgctcctgcg
    12901 ctagctagca gtgatgtagg tatctctatg agttctggaa cagatattgc tattgaatca
    12961 gcagatatcg tgttgatgaa acctgagtta acagatctct taaaagcaat gactatcagt
    13021 aaacaaacga ttcaaattat taaggaaaat ttattttggg ctttctttta taacgtttta
    13081 gcgattccag tggcaatggg tgttttacat ctttttggtg gtcccttact caatcctatg
    13141 ttggcaggct tagctatggc gtttagttca gtttcagtag ttttaaacgc acttcgctta
    13201 aaagtcttaa aataataaag ataataagac tgaattttta tgaagggaga agaatatgaa
    13261 acatacctat cgagttagtg gcatgaaatg tgatggttgt gccaaaacag tttctgataa
    13321 gttatcaagt gtgatgggtg ttgacgaggt taatgttgat ttgactaaaa atcaagttgt
    13381 cgtaagtgga aaaacattca aatgtctttt aaaacgttct ttaaaagaca caaagtattc
    13441 actagaagag gaaataaatt aataactaaa gaagtggtta cagcttttaa tttcttaatt
    13501 tgagcagttt ctcacttctt ttttattcta aaaatgataa actagaatag aattagttgt
    13561 gaggagtgca gatgtcaatc gatatttgga acattttaag tttggttgga accgtagctt
    13621 ttgcaagttc gggagctatt gtggcgattg aggaagaatt tgatgttcta gggcttttta
    13681 tcttaggttt tgtaacggca tttggtggtg gagctattag aaatgtacta attggcttgc
    13741 ctattgaaac actttggagt caaggaattg ctttttatgc agcagcagca gctattttgt
    13801 ttattatgat tttccctaac ttgttgagtg gtaagggacg ggatgccgaa gttgtatcag
    13861 atgctattgg gttagcagcg tttagtgttc aaggagcact atatgcgact caatctcatc
    13921 aacctcttag tgctgtcatt gtcgcagcag tactaactgg tgcaggtggt ggtattgtca
    13981 gagatgttct ggctggccga aaacctgggg tactaagaag tgaaatatat gcaggttggt
    14041 caatattagt ggggatcatt ttatatttta aaattgctaa aacaacaact gactattatt
    14101 tgttagtgtt agttgtaact agcctaagga tgttggggta taagaaacaa tggcacttgc
    14161 cagttgtcag accacgtaag aaatgataga ggagaaagag atgataaaat taattgctat
    14221 tgatatggat ggaacgcttt taaatgatga aaagaaaatt ccaaaggaaa atatccaagc
    14281 tattaaagaa gcaactcaag caggtattaa aattgtactg tgtactgggc gaccgatgtc
    14341 aggtatactt ccttatttta atgaactagg tttaacaaaa gaggagtata ttatcatgaa
    14401 taatggttgt agcacttata gcacgaaaga ttggcaattg attgattctg caactttaac
    14461 acatgatgaa ctcatctttc ttgaggaggt tgttaaagaa tttcccaacg tttgtttaac
    14521 gttaacagct gaaaatacat tttacgctgt tggtgaggaa gttcctgaaa ttgttgctta
    14581 tgatgctgat ttagttttta caaaagcaaa gtcaataagt ttagatgctc ttcgcaatca
    14641 agaagaaatt gtctttcaag caatgtacat gggattagat gcagatgtaa ctgcttttca
    14701 agaagctgtt gaagaagcct taatctcgaa atttagtgga gttcgaagcc aagactatat
    14761 ttatgagatt atgcctcaag gagtaacaaa agcaagaggc ttaaaatcct tgatagcaaa
    14821 attaggactg gatattaatc aagttatggc tattggtgat gctcctaacg atattgaatt
    14881 attagattta gttcccaata gcgttgctat ggggaatgca tcagatgaga ttaaatctag
    14941 atgtaaatat ataacggtgg ataataacca agccggggtt gctaaagcta tttatgacta
    15001 tgctttacgt taaaatagat atagaataat aaaaggaact ttaaacactg tttgtttagg
    15061 gttcttttgt ttaataaact aacaaaattc agaagtttac agatagctta tgctataata
    15121 gacctatgac aaacaagaat aaattacttt taattgatgg ctcatctgta gcctttcgtg
    15181 ctttttttgc tctttataat caaattgacc gctttaaaaa caattctgga cttcatacta
    15241 atgccatata tggtttccat ttgatgttga atcatatttt aggtcgtgtc cagcctagtc
    15301 atattttagt ggcttttgat gctggaaaaa ctactttccg tacggaaatg tatgcagatt
    15361 ataaaggtgg gcgtgctaaa actccggatg aatttcgtga acaatttcct tatattcgtc
    15421 aacaattaga tgtcttaggc atcaaacatt atgaattgga acattatgaa gccgatgata
    15481 tcattggtac tcttgcaaaa caagccgaag ctagtaaaga gcattttgat ataacggtgg
    15541 tttctgggga taaagattta attcaattaa cagacactaa cacggtagta gaaatctcta
    15601 aaaagggagt tgctgagttt gaagaattca cgccagctta ccttatggaa aaaatgggaa
    15661 ttacgccgag tcaatttatt gatttaaaag ctttgatggg agataaatca gataatattc
    15721 ctggggttac taaaatcggt gaaaaaactg gcttgaagtt gctatcagaa tatggttcct
    15781 tagaagggat ctatgaaaat attgaggcta tgaaacagtc taagatgaaa gaaaacctta
    15841 tcaatgataa ggagcaagct ttcttatcaa agaccctagc tacaatcaac atagcatcac
    15901 ctataacaat cggcttagaa gatatccttt atagtggacc gcaagatata aaagccttgt
    15961 ctcaatttta tgatgagatg gattttaaac agtttaaagc agctctgggt gaggagacaa
    16021 gtcaggaaga ttttgaggtt gactttactg aagtggagca attaaaacca gaaatgttct
    16081 ccgataatga tttttattac tttgagatgt taggtgataa ctaccacgta gaagatttaa
    16141 ttgggatagc ttggggaaat agcgatacaa tctacgctac tagtgatgta tcgttattgc
    16201 aagaagcttt atttaaaaaa gctttatcaa aacctatcaa aacttatgat tttaaaagaa
    16261 gtaaggtcct tttaaatcgt tttaatattg atttaccaga gccagccttt gatactagat
    16321 tggccaagta ccttttgtct actactgagg ataatctagt atcaacaatt gctaggttat
    16381 atacaaatct gccactggat actgatgatg ctgtttatgg gaaaggtgct aaacgcgcta
    16441 taccagaaaa aacacgattt cttgagcatc tagctaaaaa agtaaaagtt ttagtggatt
    16501 ctgaggctaa tgttatgcaa cagcttaaag ctaatgagca agaagaattg ctgtttgaaa
    16561 tggagcaacc tttagcgaat gttttagcta aaatggaaat tcgaggtatt aaggttaaga
    16621 aaaatacctt gaatgaaatg gctattgaaa atcaaaaagt catcgaaacc ttaacacaag
    16681 agatctatga attggctggg caagagttta atattaattc tccaaaacag ttaggaaagt
    16741 tgttgtttga aacgttaggt cttcctgtag aaatgactaa aaaaacaaaa acaggttatt
    16801 caactgcagt agatgtatta gagcgcttag ctccgatttc tcctcttgta actaaaattt
    16861 tagagtaccg tcaaatcact aaattgcaat caacctatat tattggttta caagattata
    16921 ttttagaaga tggtaaaatt catacacgtt atgttcaaga tttgactcag acaggtcgtc
    16981 tttcaagttc tgacccaaac cttcaaaata taccagttag actagagcaa ggacggctta
    17041 tccgaaaggc ttttgttcca tcagaagaca atgctgtttt attgagttca gattattctc
    17101 aaatagaatt gcgcgtgtta gcacatattt caaaagacga acatttgatc gctgccttta
    17161 aagagggagc agatattcat acttcaacag ctatgcgtgt ttttggtatc gaaaaacctg
    17221 agaatgtcac tcccaatgat cgtcgaaatg ctaaggctgt taactttgga attgtttatg
    17281 gtatttcaga ttttggtcta tcgcataatt taggaatacc tcgtaaacta gcgaagcaat
    17341 atattgacac ctattttgag cgttatcctg gaataaaaaa ctatatggaa acagttgttc
    17401 gtgaagcaaa agataagggt tacgttgaaa cacttttcca tcgtcgtcgt tcactaccag
    17461 atattaattc tcgtaatttt aacattcgcc aatttgcaga gcgcacagca atcaattcac
    17521 caatccaagg aagtgcagct gatattctta aaattgcaat gataaattta gatagggtac
    17581 ttgataaagg tggttataaa tcaaaaatgc tgttgcaagt tcatgatgaa attgtacttg
    17641 aagtccctaa cgaggagata ggagcaatca aggagttggt tacaaaaaca atggagtcag
    17701 caattagttt atcggtacct ttgattgcag atgagaacgc aggtgagact tggtatgaag
    17761 cgaagtagtg gacaacatac gtaaaggagt cattaatatg gtttatcatt ttcaaaatct
    17821 aagtgatttt atgttaaaaa attatttaac aaaagcaaag accatcgcag ttgtaggatt
    17881 gtcagaccgt caagaaactg cagcttatca agtatcaaaa ataatgcaag aagcaggtta
    17941 tcaaattatt ccagttaatc ctaaaaatgc aggacagaaa atacttgggc aaatgacata
    18001 tgcctcactt aaggatgtca ctgaacatat tgatattgtt aatatttttc gacgtagcga
    18061 gtatttacct gatattgcaa gagaattttt agaagtagac gctgatattt tctgggctca
    18121 gcttggactt gaaagtcaag aagcagagac aattctaaag caagcaggac acaaacagat
    18181 tgtcatgaat aaatgtctga aagttgaata ccaaaaatta cactcagaaa ctaactaatt
    18241 accgtgttta ataggcaaat aatataaatt atgctataat aaccatgcta aattaaagtt
    18301 cgtgctggaa aggagtccta tggacagtca ttcacatggt catagacctc tcgatgctta
    18361 tgaaaacgtc ctagagcatc tccgtgaaaa acgtattcga attactgaga ctcgaaaagc
    18421 tattatttcc tatatggtta atagtcgtga gcatccgagt gcagaaaaga tttacaatga
    18481 tttattacca gaatatccta acatgagctt ggcaacagtt tacaataacc ttaaggtcct
    18541 tgtggatgag gggtttgtga cagagctaaa actttgcaat tatagtacaa cttattatga
    18601 ttttatgggg catcagcacc taaacattgc ctgtgaagat tgtgggaaaa tcgttgattt
    18661 tgtcgatgtt gacttattgg atatttctag agaagcacac caacaaacag gttttgaagt
    18721 gactagagtt cagttagtag cttatggtat ctgtccggag tgtcagagga agcgtaagga
    18781 taaatataaa aaagattgaa acgtaattgt tcaatctttt tttgtttcta gactaagaga
    18841 aaaagtttaa gttttactaa gtaataatta agaaataatt tataattgct taaaaaatga
    18901 tttatttttt gttggtatac tctcattaac aaaaagaaag aggaatccct tatgaaaata
    18961 tcacaataca ataaatggtc aattcgccga ttgaaagttg gagctgcatc tgtaatgatt
    19021 gctagtggaa gcattgttgc tttagggcaa tctcatattg tttcagcaga tgagatgtcc
    19081 caacctaaaa caaccattac agccccaaca gctaacactt ctacaaatgt agaaagtagt
    19141 actgataagg cattatcaaa agttactacg atggagacaa gttcagaaat gcctaaaatg
    19201 caaaatatgg caaaagttga aaaaacgagt gacaaaccta tgatggtggc tacctctgtt
    19261 agaaaaatga tggcaacgcc gacacctgta gcgatgacca aaacgactag tgtagatgag
    19321 gtaaagaaat caactgatac tgcttttaaa cagacagtag acgttccggc tcattatgtt
    19381 aatgcagcaa aagggaatgg cccatttcta gcaggtgtaa atcaaacgat tccatatgaa
    19441 gcgtttggtg gagacggcat gttaacgcgt ttaatcctta aagcttcaga gggagcaaaa
    19501 tggtcagaca atggtgttga taaaaatagt ccattattac cattgaaagg cttaactaaa
    19561 ggtaaatatt tctatcaagt ttctttaaat ggtaatacga caggtaaaga ggggcaagct
    19621 ctcttagatc aaattaaagc taatgataaa catagctacc aagccacaat tagagtttat
    19681 ggtgctaaag atggtaaagt tgatttaaaa aacatgatta gccaaaaaat ggtaaccatt
    19741 aacattcctc acattacaac tgatatggaa gtaaaaaatt ctctaaagat ggcctttaaa
    19801 gaaaaagtag acgtcccagc aaaatatgtt agtgctgcaa aagctaaagg tccattttta
    19861 gcaggtgtta acgaaacgat tccatatgaa gcgtttggtg gagatggcat gttaacgcgt
    19921 ttaatcctta aagcttcaga gggagcaaaa tggtcagaca atggtgttga taaaaatagt
    19981 ccattattac cgttaaaaga tttaactaag ggtaaatact tctaccaagt ctctcttaat
    20041 ggcaatacag ccggtaaaaa aggccaagct ctcttagatc aaattaaggc aaatggaagc
    20101 catacttatc aagcaactat tactatttat ggtacgaaag atggcaaagt tgatatgaat
    20161 actattttag gacaaaagac agtgatgatt catattaatg tcgctaaaaa agatatgaat
    20221 agcacatcaa tgatgatgaa aaaagataag atgacaatgc ctatgaagaa agaaatgact
    20281 tcatctaaaa taaataccgg tatgatgatg tctaacaaca agatgtcagc taatatgcaa
    20341 atgtcatcac aagccaaatc aaatgataaa gctggtaaaa aaatgtctat gatgtctaaa
    20401 aatttaccta atacaggtga aacaaagcaa caaaatgtag gtgtgctagg tatgcttagt
    20461 ctagcctttg cgacaggtct aactgcgcta ggccttaaaa aatctaaaca aagataggga
    20521 tagtatttaa ttcagaacac caaatttgtt taaaattatt gagggttatc gtggtaaaat
    20581 agtgttatac tagaggtata gcactatttt gactcattag gagagtagaa tgacacaaaa
    20641 attattatta gttgacgatg aatttgaaat tattgatatt aatagacgtt atctagagca
    20701 agcaggatat gaagtgtcag tagctgcgga tggcatagaa gcactcaaag aggtagatga
    20761 gaatcgtttt gacctaatta ttagtgatat tatgatgcct aaaatggatg gctatgattt
    20821 tattagtgaa gttctagttc gagaacccaa ccaacctttt ctctttataa ccgctaaagt
    20881 atctgaacca gataaaattt actctttgag tatgggtgca gatgacttta tttccaagcc
    20941 tttcagtcct agggaattag ttttacgtgt taaaaatatt ctaagacgta tctatgggaa
    21001 tcatcaacaa tctgaagtat tgacgattgg tgatttggtt atcgaccaaa aacaacgttt
    21061 ggtaatggta gactgcaata caatttcctt aaccaataaa tcatttgact tgctttggat
    21121 attagcaaat catttgaatc gtgttttttc aaaaacagag ctttatgaac gtgtctgggg
    21181 cgaagaattt ttagatgaca ctaatacttt gaatgttcat atccatgcct tacgcaatga
    21241 ccttgctaaa tttagtacag acaatacccc aactatcaaa acagtttggg gattagggta
    21301 taaattagag gaataaaatg aaattaaaat attatattgt tattggttac cttatttcta
    21361 tgttaataac tgtagcaggt gttttctttg ggcttaatca tatgctgatt gagactcgag
    21421 gtgtttacta catcctttca gttacaatta ttgcctgtat cgtaggtggt atagttaatt
    21481 tgttcttatt atcaagtgta tttacatctt tgaaaaagtt aaaacaaaaa atgaaggata
    21541 tttctcaaag acgttttgat acaaaagcac aaatttgttc accacaagaa ttcaaggatt
    21601 tagagacagc ctttaaccaa atgtcttcag agttagagtc aacgtttaaa tctctaaacg
    21661 agagtgaacg cgaaaaaaca atgatgattg cacaactttc acatgatatc aagactccaa
    21721 ttacctctat acaatcaaca gtagaaggta ttttagatgg tattatatct gaagaagaag
    21781 ttaactatta tctcaatacc atttctcgtc aaacaaaccg tcttaaccat ctagttgaag
    21841 aattaagttt tattacatta gagactatgt cagacacggc tgaacctcat aaagaagaaa
    21901 caatttatct ggataaacta ctgattgata ttttatctga atttcaattg gtgtttgaaa
    21961 aagaaaatcg gcaagtaatg attgatgttg cgccagatgt ttcgaaatta tctagtcaat
    22021 atgataaatt atcacgaatt ttattgaatt tgattagcaa tgcagtcaaa tattctgatc
    22081 ctggaagtcc cttgactatc aaggcctatt caaatcgtca agatatcgtt atcgatatta
    22141 ttgaccaagg ctatgggata aaagatgaag atcttgcttc tatttttaat cgtttttatc
    22201 gtgtagaatc ttcacgaaat atgaagacag gaggacatgg cctaggtctt tatattgcgc
    22261 gacaacttgc acatcaactg aatggtgata ttttggtcga aagtcagtat caaaaaggca
    22321 gtaagttctc tttagtactt aaattacaaa aatagctagg aattattcct agctattttt
    22381 tgtaatgttt ctataaaaga ttaagcgcgc agtagtttgg taaggaagga gatagatata
    22441 gactaatccg aaactgaaga ttgtaaggaa atgccaacca ataaaactta agtctaaaag
    22501 gaagagtcta agtttattac cattcattag ttctcgacta gcaactagta ctttagatgg
    22561 tccctcatag tttccacttt ctaattgagt gtaaaatact aaagttgaga tactatagga
    22621 gtaatattgt tgaacgagtt ttataactgc cttagtagta attaggctac taacaactat
    22681 taaaacaggg gcaaggataa ggttaccact caaaagtagc ctaaaacttg aaatgatgat
    22741 tagtagacca aaagtactta gtagataaaa gaaagataaa attagtcctt ttagaacaag
    22801 tactgagagt aatttccaga aaatatggct tgaaaataca taagtattgt ccgagaatct
    22861 aacgtttagt ttctgatttt tgacgacatc taacattaca aaactgactg aaataataaa
    22921 aagtgtgtaa aagaaataaa ctggaagagg tacaaaaaaa ccaagtagtg tcatattatt
    22981 atattgataa taaaatgata tatataacgt aataatacta agaagaatcg ggagtaaaaa
    23041 aagttgattc ttaccttgaa gattgcttaa tatagttttt gcctcatttt taatttctga
    23101 attcgtcata tctcctccat acataaaagt acaattcatt tcattataac acattcctta
    23161 agatgagcat gacaagagta catttttttg ataaaatctt aatggctaaa aaagaaatta
    23221 atacaaagta ttacatttag atattgatta gcactaaagc ccatgactgt ttttcatgga
    23281 agcaaggaga aaacgatgac tgatcatcca ataaaatatc gattaattaa acaggaaaaa
    23341 cacacaggag ccagacttgg tgaaataatc acaccacatg gtacatttcc gacaccaatg
    23401 tttatgcctg ttgggacaca agcaactgtt aagacacaat ctcctgaaga actaaaagaa
    23461 atgggttcag gaattattct atcaaatacc taccatttat ggttacgtcc aggtgatgaa
    23521 ttgattgcta aagctggagg tttacataaa ttcatgaact gggatcaggc tattctaaca
    23581 gacagtggag gctttcaggt ttattctttg gctgatagtc gaaacatcac tgaagaagga
    23641 gtaaccttta aaaaccattt aaatggtgcg aaaatgtttt tatcaccaga aaaagctatt
    23701 tcaattcaaa ataaccttgg ttctgatatt atgatgagtt ttgatgagtg tccacaattt
    23761 taccagcctt atgattatgt aaaaaaatca attgagcgaa caagccgttg ggcagaacgt
    23821 ggattaaatg cccaccgccg tcctcatgat caaggactat ttggtattgt tcaaggggca
    23881 gggtttgagg atttacgtcg tcaatcagca cgagatttag tatcgatgga ttttccaggt
    23941 tattcaattg gagggcttgc ggtcggagaa acacatgatg agatgaatgc agttcttgat
    24001 tttactgttc caatgcttcc gaatgataag cctcgttatt tgatgggagt tggtgcacca
    24061 gatagtttaa tagacgccgt tatccgagga gtagatatgt ttgactgcgt cttacctact
    24121 cgtattgctc gaaatgggac atgtatgaca agtcaaggtc gtttagttgt taagaatgct
    24181 aaatttgcag aagattttac gcctcttgat ccaaattgtg attgctatac ttgtaaaaat
    24241 tatacacgcg cttatatccg tcacttattg aaagcagatg aaacttttgg tattcgtttg
    24301 actagttacc ataatcttta tttccttgtc aatcttatga aagatgtcag acaagctatt
    24361 atggatgata atcttctaga gttccgtcaa gattttatgg agcgctatgg ttatggcacg
    24421 aataaccgta atttttaaaa aagttcctgg taaaacaggg ctttttttga tctgtttgtt
    24481 aaccgcgttt taaaaaaagt tgacaataga aaaacttttg tataatatgt tataggagga
    24541 atcatgatgc aagagtactt tggtattggt cttgatagta gcagccgctg ttaccactat
    24601 catactaaat tggatattgt cgctttaaaa tgtgctgttt gtcaaaaata ttatgcttgt
    24661 tataaatgtc atgatgcact cgaggagcat tgttttgcag caacaaaatc tgatgaaatg
    24721 tttcctgtct tatgcggttc ttgtcgtcag atgttgacat taaaagagta taaaactggt
    24781 ttttgtcctt attgtcgtat gctttttaat ccaaattgtc aacgccatga ttctatttat
    24841 ttttcgaagg agtaattttt aatgtttcac acaaaaagcc taatccagat ggctatgatg
    24901 gctactttat tggtggtact tggtttcatt cctgctctac cacttggttt cattccggtc
    24961 cctatcgtct tacaaaacct aggggttatg atggctggtg ttattttggg cggaagaaaa
    25021 gggagtttat ctattttgct cttctttctt gtcggtttag ttattcctgt attctcaggt
    25081 ttacgttcga ctatacctgt attgacagga ccaacagctg gttatgttat tgcttggttg
    25141 tttgttcctt tgcttatctc atatggtgta aaattagtaa ataaaaagaa ttttatagct
    25201 attttcctta ttgtatggct agcaggggtc ctttttgtag atgtagcagg agcagtttgg
    25261 ctggcatctt atatgaacat cccattagat aaatcacttc ttcaaaactt agtatttctc
    25321 ccaggagata caataaaagc cattatagct gctgttgttg ctgttaaata taaagatagt
    25381 tttttcttga ctgaaaaata atcaagattg ctatatttta gatggactag tagatctaaa
    25441 aatttagatt tactagtctt ttttgtgcat aaaatctaaa acagttattc ttaacaaaat
    25501 ttggtataat agcattaaat cagtaaggag gggattatca tgaaattaac aacgttaggt
    25561 tgttggggtg gttatcctta taaagatgga ggaaccacaa gttttctatt aactggagaa
    25621 aataattttc acttattaat agatgcggga agtcgttcaa tcaatgagtt agaaaaagaa
    25681 ataagcccgt tggatttgga tgcagttatc atcagtcatt atcatccaga tcatgtagca
    25741 gatttgggtg tgttgtatca ttatcgtttg ctatacccta aagataaatg ggaacccaaa
    25801 gtattgccag tttatggaca taatgaggat gattatgagt tttctaaatt gacattacct
    25861 aacgttgctc aaggaattgc ttatcatacc aatggagttg agcaagttgg tcccttcaat
    25921 attacgttta tcaaaacagt ccacccagtc gtctgctatg ccatgaggat tacagaaaga
    25981 gaaactggtc aagtattagt ctttacagga gattctgggt attttgctga ccttgtacct
    26041 tttacaaaag gcgcagattt atttttagca gacgtttatc tttacgaggg aaatgaaaat
    26101 catattgcac atttgactag taaagaagca ggtgagattg cttataacgc taaagttaag
    26161 aaattagttt taacacatgt tccgcagttt ccgggagaag gaataaaccc cgaaaatcat
    26221 ttggaagaac ttcgactaca gtctgaaaag tatgcaggtg gcatctctgt tgatttagct
    26281 cagccacata aatcttggga cttaggaaaa ctataatgca gaaatttacc caagaagaaa
    26341 aagaatggtt tatgacagaa gctctaaagg aggcaaaatc ctccttagag aaagaagaaa
    26401 ttcctatagg ttgtgtcatt gtaaaagatg gtcatattat tggaagagga cataatgctc
    26461 gtgaagagtt taataaagct attttacatg ctgaaatcat ggctattaac aatgcaaatg
    26521 agaaggtagg gaattggcgc ttattagata ctactttatt tgttactgta gaaccttgtg
    26581 taatgtgtag tggagctatt ggcttagcta gaattcccca cgttatatat ggcgctaaga
    26641 atacgaaatt tggagcagcg ggaagtctct atgatatttt aagtgatagc cgacttaatc
    26701 atagagttga agttgaaacc ggcattttag aagatcaatg cgctaaaata atgcaggatt
    26761 tctttcgaaa aggacgacaa cgcaagaaag aagctaaact aagtgaaaaa actaagattt
    26821 gatagcttgc aataaaatat tagttttgtt ataatatctt tggagcaaca tttactcgtg
    26881 aagtgggtca ggggaggaat ccagcagccc taagcgatgc taaatgtgtg ctctttttat
    26941 tttcaaaagt caaagtgttt ttggaaataa ttatgaaagt ttcataaaaa ttggtagcgt
    27001 ttcaaaaaat atgaaaatac agttaaaaaa ccttgaaaaa aaactcacaa aagagtaaga
    27061 tgatagtgaa acataattgt taggagaaat aaaatgacac atattacatt tgactattca
    27121 aaagtcttgg gtcaatttgt aggcgaacac gagttagact acctacaacc acaagtaagt
    27181 gcagcagatg ctttccttcg tcaagggact ggtcctggat cagattttct cggatggatg
    27241 gacctcccag aaaactatga caaagaagaa ttttctcgca ttcaaaaagc cgctgaaaag
    27301 attaaatcag atagcgaagt actcgtggtt attggtattg gtggttcgta ccttggtgca
    27361 aaagcagcaa ttgacttttt gaataatcat tttgctaatt tgcaaaccgc agaagaacgt
    27421 aaagcgcctc agattcttta tgctggaaat tctatttcat ctacttacct tgccgattta
    27481 gttgaatacg tccaagataa agaattctca gtaaatgtca tttcaaaatc aggtacaaca
    27541 actgaaccag cgattgcttt ccgtgtattt aaagaacttc tagttaaaaa gtacggtcaa
    27601 gaagaagcta ataaacgtat ctacgctaca actgataaag ttaaaggtgc cgtaaaagtt
    27661 gaagcagatg ctaacaattg ggaaacattt gtagttcctg ataatgttgg tggtcgtttc
    27721 tcagtactga cagctgtagg tcttcttcca attgctgctt caggagcaga tattactgct
    27781 cttatggaag gcgcaaatgc tgctcgtaaa gatttatctt cagataaaat ctctgaaaac
    27841 attgcttatc agtacgctgc agttcgtaat gttctttatc gcaaaggtta tatcacagag
    27901 attttagcta actacgaacc atcattacaa tactttggtg aatggtggaa acaattagca
    27961 ggtgaatcag aaggtaaaga tcaaaaaggt atctatccta catcagcaaa cttctcaact
    28021 gatcttcact cacttggtca atttattcaa gaaggttacc gtaacctctt tgagacagtt
    28081 gttcgcgttg agaaacctcg taaaaatgtg actattcctg aattaaccga agatttagat
    28141 ggtcttggtt atcttcaagg aaaagatgta gattttgtta ataaaaaagc aacagatggt
    28201 gtgcttcttg ctcatacaga tggtggggtt ccaaatatgt ttgtaacgct tcctacacaa
    28261 gacgcttaca ctcttggtta cactatttac ttctttgagt tagcaattgg cctttcaggt
    28321 tatcttaact cagtaaatcc atttgatcaa ccgggggtag aagcatataa acgtaatatg
    28381 tttgctcttc taggtaaacc tggattcgaa gagcttagcg ctgaattgaa tgcacgtctt
    28441 taaaaatatc taactaaaaa gttcctcaaa gagaggaact ttttagtttt gttgtaggat
    28501 aactaaaaac ccactagcac tggtagcgat agtgggttgg aaaaagggtt gagaaaagtt
    28561 attaactttt ctcaggagat ttatgaaaaa ggaaaataat ttaggataat attttcctaa
    28621 cggctaggat aaaccgctta ttttaggatg atgctagtat acagtagaaa ccttaaataa
    28681 accttaaaaa ttcatacttt atcattttat tttcccctat actatttttg tataaatggt
    28741 caatattaat ggtatcatag atatatggaa aagaaattat taagaaaaga agttttgata
    28801 accttaaaat ctcaacctca agcttacaaa tcagaagttg actctaaact tttagaggct
    28861 tttataaaaa ctaaagctta tcaaaatagt tgtgtaattg ctacttacct atcttttgac
    28921 tacgaataca atacacaact tcttattaaa caagctttat gtgatggcaa acgtgtcctt
    28981 gttcccaaaa cttatccaaa aggtaaaatg atttttgtgg actatcaaaa agataacttg
    29041 agggcaacac cgtttggctt attagaacct gttaatgata gagctgttga aaaagctagt
    29101 atagacttaa ttcatgtgcc cggacttatc tttaacaata aaggttttcg tataggttat
    29161 ggtgcaggtt attttgatcg ctatttgagt gattttgaag gtgatactat tagtactatt
    29221 tataggtgtc agagacagga ttttgtagaa gagaagcatg atgtagcggt taaggaggta
    29281 ttatgtctat gaagaagttc gctaaagagt atccgacgac agtactcttg gtcggtctga
    29341 caacccttgt ttttttattg atgcaactaa cttatggaag tcaagcagag tctagccaag
    29401 ttatttttca atttggtgga atacaaggtg attatctaaa agcatatcca acaaatctat
    29461 ggagactaat aagtcctatc tttgttcata ttggttggga acatttttta ttaaatggtt
    29521 tagctctgta ttttgtggga caaatggggg aaagtatttg gggaagtttg agatttctaa
    29581 ttctctatat tttatctggc ttaatgggta atatttttac gctatttttt actccacacg
    29641 tggtggcagc aggagcctca acgtctttat ttggtgtttt ttctgctata gcaatcgcag
    29701 gttattttgg aaaaaatcct tatttaaaac aagttggaaa atcatatcaa gtgatgatct
    29761 tgttaaattt attttttaat atcttcacac ccggagtgag tttagcaggc catgttggtg
    29821 gtttagttgg tggagtattg gtggctattt ttttgacaaa acaaaatggt tctttattat
    29881 ttaaaacttg gcaaagtatt ttagctttaa tgatatttat tattgtatcg atcagtttaa
    29941 taggtctatc gttggtataa ttctacggct atagtctcta aaaccaaact ttctttggga
    30001 aaaatagaat aaagtttgct aaatgatttt tttatgttat gatagaaatc tgtttttatg
    30061 tgactataac ttattaggag gttcattctc ggtgaacaga aaaattattg gcttaactct
    30121 actatcttta tctgtactaa cattaacagc atgtggtaat cgttcagata agtctgcaaa
    30181 taaatcagat atcaaagttg cgatggtaac taatcaaggt ggagtagacg ataaatcgtt
    30241 taatcaatca gcttgggaag ggctacaaaa gtggggaaaa aagaaggggt taacaaaagg
    30301 taatggcttt gattatttcc agtcatctaa tgaatcagat catgctaata accttgatac
    30361 ggcagcctct agtggctata atttaatttt tggtattgga tttggtattc atgatactat
    30421 tgaaaaagta tcagaaaata ataaagatgt taaatacgtt attgtagatg atatcattaa
    30481 aggtaaagaa aatgtagcta gtgttacttt tgcagacaat gaagctgctt acttagctgg
    30541 agttgctgca gctaagacaa ctaagacaaa aacagttggt tttatcggag ggatggaagg
    30601 cgtcgttgtt aagcgatttg aagcaggatt taaggctgga gtaaaatcaa ttgatccggc
    30661 tattaaagtt gcggtatctt atgcaggctc atttacagat gcagctaaag gtaaaacgat
    30721 tgctgccaca cagtatgcaa caggtgttga tgttatttac caggctgctg gtggtacagg
    30781 agcaggaatt tttagtgagg ctaaaactga aaatgagacc cgcaaggaat ctaacaaagt
    30841 ctgggtaatt ggtgttgaca gagatcaaag tcaagaaggc aattatgttt caaaagatgg
    30901 taagaaagca aactttgttc tggcatctac tataaaagag gtggggaaat ctctccaatc
    30961 tgtagcagaa ttaacagaga aaaaacaata tccaggaggg aaagtaacag tttttggtct
    31021 aaaggatagt ggtgtagata ttaaagagca tcaattgtct agtgagggat ctgttgctgt
    31081 caaaaaagct aaagagggga ttgtttcagg gaaaatacaa gttcctatga aatgataaaa
    31141 gtgtggagga acaataagtt cttcgtcaag tacaataaaa aagtttccta aataggaaac
    31201 ttttttattg tacttgactc atattttatt tcttagatgt tttttctaga gattttccta
    31261 aatcaatgat atatttcttc aagtcatctt taacttgtgg gtgttgaaga gcataatcaa
    31321 tagaagtttt cataaaacca aacttgtcac caacgtcata acggtcccct gtaaattttc
    31381 tagcaaaaac acgttgcgtt ttatttaaag tatcgatagc atcggttagt tgaatctcat
    31441 ttcctgcacc aggtttttga gtttctaaaa tattaaaaat ttctggagtt aacaagtagc
    31501 gaccaatgat tgccaaatta cttggagcct cttcgggaga aggtttttca acgaaagtgt
    31561 taacgctata tagaccatta actccttctc cttgtggagc aataacacca taagcagaaa
    31621 catcttcatg tggaacttcc ataacagcaa ttgttgatgc atgagtagct tcaaagtcat
    31681 taattaattg tttggtgaga ggaataactt tattattggt aatatccatt agatcatcac
    31741 ctagcataac aacaaaaggt tcattaccaa cgaaggcttt tgcttgtagc acagcatctc
    31801 caagcccacg aggatgactt tgtcgaataa aatggagtcg aatgccagtt gtttcatcaa
    31861 caagtttgag aagttcgttt ttccctttct cttttaggtt atattctaat tcaaaatttg
    31921 aatcaaagtg gtcttctata gaacgttttg atttaccagt tacaactaaa atatcttcaa
    31981 ttccagattt tagtgcttct tcaacaataa attgaatagt tggtttatca acgattggaa
    32041 gcatttcttt agctaaagct ttagtagcag gtaaaaaacg tgttcctaaa ccagcggcag
    32101 ggataactgc ttttctgact ttcatatata gctcctttaa atagtattaa tataaacgat
    32161 attagtgcca ttcattctct gaacgaaatt cattggacat catatctaaa atgctatctt
    32221 tgatattagc cccttcatat attgatttat aaatagcttc tgtaatgggc atgtaaacat
    32281 ttaagttttg ggcgatttcg taagcaacct tagttgttga gataccttca ataaccatcc
    32341 ccatattttt ttcaatatct tcaagtttct caccacgtcc taaagcatct cccgctctcc
    32401 agttgcgaga atgaacagat gtaccagtca caattaaatc tccaactccg gataaaccac
    32461 tgaaagtgag agggtcggcc cctaattgta caccaaggcg tgttatctca gctaaaccac
    32521 gcgtaataat agctgcttta gcattatcac cataaccaag accgtgtagt gctccagcac
    32581 caacagcaat aatatttttt agagcaccag ctgtttctac tcctacgaca tcagtgttag
    32641 tataaagtct aaaataatga ttactaaaca atttttggac atacttagct gcttcaatat
    32701 cttttgaagc agcagtaatc aaagtaatat cacgaacgat ggcttcttca gcatgactgg
    32761 gacctgaaac aacaacgata tcactacgat attgttcaga gatttcttct tctaaaattg
    32821 tagaaagtcg ttcatgagtg ccaggttcta gccccttaga agcatgcata attacaactt
    32881 tatgctttaa aagactagcc acttgtttag caacaagtct tgtaacttta gttgggacga
    32941 cgaatagaat gctgtcaaca ttattaatcg cttcctcaag attagtgtag gcttttattt
    33001 tggaatctaa ggtaatatct ttgaagtaac gttgattagt atggttagta ttaatctctt
    33061 ctatttgttc aaccacgttt ccccataagc gtacctcatg tccattgtca tttaatactt
    33121 gtgcaagagc agtcccccat gaaccaggtc caagaacggc aattttttgt tttgtcatct
    33181 tattccttct ttctaataat aaggcatata gcgctaacat tatatcatac tctaatacac
    33241 tttgtcttgt ttagtctgtt gtcgatgata gtagcattag aaaagaattg agtcttttag
    33301 gttatttatg gtataataaa accaatttag ataaatggag cactattttg aaaaaaacct
    33361 atcgggtaaa aagtgataag gattttcaaa tgattttcag cagaggtaaa aatgttgcta
    33421 atcgaaaatt tgttatctac tatttagaaa aagaacaaaa acactttcgt gtgggaatct
    33481 ctgttagtaa gaaattagga aatgctgttg ttcgtaacgc tgttaaaaga aaaattaggc
    33541 atgttttact ctctcagaaa acagcgctgc aagattatga ttttgtcgtg attgcgcgta
    33601 aaggtgtaga ggaattagac tatcaagcat tagaaaaaaa tcttatacac gttttaaaaa
    33661 tagcaggact catctagaaa ggtataaaat tgaagaaaaa acttaaaaca tttagcttaa
    33721 tattgctgac aggttcttta ttggttgctt gcggacgagg agaagtatct agtcattcag
    33781 ctacattatg ggagcaaata gtttatgcat ttgctaaatc aatacaatgg ctctccttta
    33841 accattcaat tggtttggga ataatcttat tcactctaat aatcagagcg attatgatgc
    33901 cgctttataa tatgcaaatg aaatccagcc aaaagatgca ggaaattcag ccaaggctca
    33961 aggaattaca aaaaaaatat ccagggaaag atcctgatag ccgtttaaag ctaaatgatg
    34021 aaatgcaatc tatgtataaa gctgagggag ttaacccata tgcaagtgta ttacctcttc
    34081 taattcagtt acctgtccta tgggctcttt tccaagcttt gactagggtt tctttcttaa
    34141 aagtaggaac gtttttgagc ctagaattat cacagccgga cccatattat attttacctg
    34201 tactggcggc cttatttacc tttttatcaa cttggttaac taacaaagca gctgttgaaa
    34261 aaaatattgc tttaactctt atgacttatg ttatgccgtt tatcatattg gtaacaagtt
    34321 ttaactttgc tagtggtgtt gttttatact ggacggtgtc taatgctttt caagtatttc
    34381 aaattctatt attgaacaac ccatacaaga ttataaaggt tcgagaagaa gcagtaagag
    34441 tggcacacga aaaagaacaa agagttaaac gtgctaagag aaaagcaagt aagaaaagaa
    34501 agtaggaaaa acatggtatt atttacagga gcgactgtag aagaagctat tgaaaaagga
    34561 ctacaagagt taaacatttc acgattgagg gcacatataa aagttgtatc acgtgagaaa
    34621 aaaggttttt tgggctttgg taaaaagcca gcaaaagttg aaatagaagg tatcaccgac
    34681 gaagtaactg atattaatga atcggtagcg ttaaaaaata ttaagaatgt accgtctagc
    34741 gtagatgtgg tggaagaata tatagaggag gttgatgaaa cgcttgaaaa agaagacgta
    34801 tcacaaccag aacttccaaa aattgatgat aaaaacgttg taactacaag tgaagctatt
    34861 gaaaaaattg acctacttcc taatattgaa gtagcagctg ctcaggtgac taaatacgtt
    34921 gaaaatatca tctatgagat ggatttagat gcaacaatag aaactacgac aagtaagcga
    34981 caaataaatc tccaaataga aacgccagaa gctgggcgca tcattggtta tcatgggaaa
    35041 gtattaaaat cgttgcagtt gttagcacaa aattatcttc atgaccgctt ttctaagagt
    35101 ttttcagtat ctattaatgt ccatgactac gttgagcatc gtatagaaac tttaattgat
    35161 ttttctaaaa aaatagctag acgtgtttta gagactaatg agccttacca tatggatcct
    35221 atgagtaaca gtgagcgaaa aactgttcat aaaacaattg caactataga gggtgttgaa
    35281 agttattctg aaggtaacga tcctaataga tttgtagtag ttactaagaa gtaaaaagag
    35341 gtttcacctc tttttt
//
LOCUS       DATRUK010000015        34284 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.015, whole genome shotgun sequence.
ACCESSION   DATRUK010000015 DATRUK010000000
VERSION     DATRUK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34284
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.015"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..550
                     /locus_tag="VBE34_000682"
     CDS             <1..550
                     /locus_tag="VBE34_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEO7263420.1"
                     /translation="KEQQEKIGYLGFIYPNKKNNKGRKDIFILTNSDQIEHLEVLVHH
                     LSDYHFHIAAYTEMSFKLMSFSQEQNVTLYPNISRTDLDNLFEICDIYFDINHGNEVD
                     DVIRRAFEYNHLIFAFDNTCHNRELVLDSNIISHTTCEQLINLMKNLSGSIMYLLEQQ
                     REQTSNETKERYKEILGGYGNA"
     gene            543..710
                     /locus_tag="VBE34_000683"
     CDS             543..710
                     /locus_tag="VBE34_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263421.1"
                     /translation="MPKRNELLNKEIKMSIDKLRYKEPESEHDKRPTFYLVVLILVTV
                     AVILSLFKYFL"
     gene            764..1759
                     /locus_tag="VBE34_000684"
     CDS             764..1759
                     /locus_tag="VBE34_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO7263422.1"
                     /translation="MRTYITNLNGHSITSTAQIAQNMVTDIAVSLGFRELGIHSYPID
                     TDSPEEMSKRLDGICSGLRKNDIVIFQTPTWNTTTFDEKLFHKLKIFGVKIVIFIHDV
                     VPLMFDGNFYLMDRTIAYYNEADVLIAPSQAMVDKLQSYGLTVKKILVQGMWDHPTNI
                     TLQAVNHKKLVHFPGNPERFNFIKNWRIPTELHVYTDHNMQLPTTVVKEPYQSDEQLI
                     MKMSEGGYGLVWMDDRDKQYQSLYCPYKLGAYIAAGIPVIIQKGIANQDIIEKNNLGF
                     IIEKIDDISNIVESTTEEEYMEIVSDVRRFNPLVRQGYFTRKLLTDAVFSALNSM"
     gene            1767..3479
                     /locus_tag="VBE34_000685"
     CDS             1767..3479
                     /locus_tag="VBE34_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEO7263423.1"
                     /translation="MKELEEIVVFPINKSLDYILENHSSVIRFGDGEFDIIMGKSIPY
                     QDYNPELADRLLQLLHKQSTNDLLVCLPDMFQNRQRYNQSAQLFWKNHFQKFGNFYRQ
                     HCYQDWYGSSFISRPYMDLQDKESAVESFKKLKLLWDKRDILVVEGETSRSGVGNDLF
                     NNCNSLKRIICPSKNAYIKYDEILEAVERYAQGRIILLMLGPTAKILVRDLSDRGFQA
                     IDIGHIVSEYEWYKMGATQKIKFSHKHTAEFNDDENINLIDSQNYIDEIVKTITIDDT
                     EFRDTELISIILPVYNMSGSLSRCLDSILRQVYSTFEVILVNHGSTDGSGNICEKYAM
                     KDERIRLFHEEHQVITSVWNFALEKSKGKYVTFVNPDDFLDESYLNYLYNKLRANKSD
                     ISATTYTFFNEEEKCFVYFATDDNYFEKTFFAQEWLEQEHSYHDNLKQIFPLLSMKLF
                     RKSLFNNITFPLEDLTGEYTLYNVYLLSRHITFSHIGLYITSQVFSHTEIKRFTGHDI
                     EKMLSRKEERLALLTMVGFNSEKYIKNYKETLLNYQSESLNIGDINLYNKIKQKIALI
                     DYCY"
     gene            3603..4952
                     /gene="gorA"
                     /locus_tag="VBE34_000686"
     CDS             3603..4952
                     /gene="gorA"
                     /locus_tag="VBE34_000686"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEO7263424.1"
                     /translation="MSKQYDYIVIGGGSAGSGTANRAAMYGAKVLLIEGGQVGGTCVN
                     LGCVPKKIMWYGAQVSETLHKYSSGYGFEANNLSFDFTTLKANRDAYVQRSRQSYAAN
                     FERNGVEKIDGFARFIDNHTIEVNGQQYKAPHITIATGGHPLYPDIIGSELGETSDDF
                     FGWETLPNSILIVGAGYIAAELAGVVNELGVETHLAFRKDHILRGFDDMVTSEVMAEM
                     EKSGISLHANHVPKSLKRDEGGKLIFEAENGKTLVVDRVIWAIGRGPNVDMGLENTDI
                     VLNDKDYIKTDEFENTSVDGVYAIGDVNGKIALTPVAIAAGRRLSERLFNHKDNEKLD
                     YHNVPSVIFTHPVIGTVGLSEAAAIEQFGKDNIKVYTSTFTSMYTAVTSNRQAVKMKL
                     ITLGKEEKVIGLHGVGYGIDEMIQGFSVAIKMGATKADFDDTVAIHPTGSEEFVTMR"
     gene            complement(4995..5447)
                     /locus_tag="VBE34_000687"
     CDS             complement(4995..5447)
                     /locus_tag="VBE34_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263425.1"
                     /translation="MSKGYSRQQKKSTKTNTDSTQSPAKHIKTGFSALQKTIALVGSV
                     LSIIVASITITNAINNHKDSSKTDSSTSKTVIIRESGQNSSNQVSTEKDETRASSSQT
                     LENSSASSTPSSSSNSAANNDQTSSSTNNDQTNNTTTDTSTIENTTTP"
     gene            5699..6844
                     /locus_tag="VBE34_000688"
     CDS             5699..6844
                     /locus_tag="VBE34_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO7263426.1"
                     /translation="MIYFDNSATTIPYPEVLRTYQEVASKIYGNPSSLHELGTTSSRI
                     LEASRKQIASLLELKANEIFFTSGGTEADNWVIKGLAFEKQHFGNHIIVSDIEHPAVK
                     ESAKWLGEYGFEIDYAPVDDKGFVDVEALVKLIKPETILISIMAINNEIGSIQPIKAI
                     SDLLSDKPTISFHVDAVQAIGKIPTKDYLTERVDFASFSSHKFHGVRGVGFLYIKEGK
                     RISPLLTGGGQESDLRSTTENVAGIAATAKALRMVMDKEVVAIPKISKMKTIIHDELA
                     KYEDITLFSGKEGFSPNIITFGIKGVRGEVLVHAFEGHDIFISTTSACSSKAGKPAGT
                     LIAMGISTKLAQTAVRISLDDDNDMGQVEQFLTIFKQVYEKTKKVRG"
     gene            6846..8060
                     /gene="thiI"
                     /locus_tag="VBE34_000689"
     CDS             6846..8060
                     /gene="thiI"
                     /locus_tag="VBE34_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEO7263427.1"
                     /translation="MQYSEIMIRYGELSTKKKNRMRFINKLKNNMEHVLSIYPDVSVK
                     TDRDRGHVYLNGTDYHEVAESLKEIFGIQAFSPSFKVEKNVDTLVKAVQEIMTSVYKD
                     GMTFKITAKRSDHSFELDSRALNHTLGDAVFSVLPNIKAQMKQPDINLKVEIRDEAAY
                     ISYEDIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAHDLTRKLTKFGGNIQFIEVPFTEIQEEIKEKAPEAYLMTLTRRFMMRITDRIR
                     EDRNGLVIINGESLGQVASQTLESMQAINAVTATPIIRPVVTMDKLEIIDIAQKIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNTEQYEKRMDVEGLVERAVAGIMVTTIQPQAD
                     SDDVDDLIDDLL"
     gene            8162..9340
                     /locus_tag="VBE34_000690"
     CDS             8162..9340
                     /locus_tag="VBE34_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEO7263428.1"
                     /translation="MARIPYQKTLIFCVAVIIAIFILGLSKDLAQSKGQKVANNNTVK
                     TARVVANGDILLHDVLYASARQPDGTYNFTPYFKEVKSWIESADLAIGDYEGTISSEY
                     PLAGYPLFNAPNEIATTMKETGYDVVDLAHNHILDSQLAGAINTVKTFNRLGLDTIGV
                     YLKDRNKEDILIKRVNGIKIAILGYSYGYNGMEANVSKSDYEKHMSDLDTKKIKQDIK
                     KAEKEADITIVMPQMGIEYQKKPTTEQVILYHSMIKWGADIIFGGHPHVVEPSEVIKK
                     DGQKKFIIYSMGNFISNQRLETVDDIWTERGLLMDVTIEKKGQKTVIKKVKAHPTLVE
                     AKANGRYVEEGFPAYDYRTLVLEDYIEGGKKRSTIDQTMKDKVDKAYKEMNELVNLKW
                     "
     misc_feature    9416..9495
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            9530..9844
                     /gene="rplU"
                     /locus_tag="VBE34_000691"
     CDS             9530..9844
                     /gene="rplU"
                     /locus_tag="VBE34_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEO7263429.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKLDVEAGAEVTFNEVVLVG
                     GETTKVGTPVVEGATVVGTVEKQGKQKKVVSYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            9851..10189
                     /locus_tag="VBE34_000692"
     CDS             9851..10189
                     /locus_tag="VBE34_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEO7263430.1"
                     /translation="MIKATFTRNQSGYLYSAEISGHAGSGEYGFDVICAAVSTLSINF
                     INSLEALTTCQAQLIINDVEGGYMKIDLSSIPQHKEDKVQLLFESYLLGMTNLSKDSS
                     EFVSTVVMTH"
     gene            10211..10504
                     /gene="rpmA"
                     /locus_tag="VBE34_000693"
     CDS             10211..10504
                     /gene="rpmA"
                     /locus_tag="VBE34_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEO7263431.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGANVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            10720..11625
                     /locus_tag="VBE34_000694"
     CDS             10720..11625
                     /locus_tag="VBE34_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7263432.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAAKLFVSQPSLSVAVRDLETELG
                     FQIFTRTTTGAVLTNQGMTFYENALEVVKSFDSFEKQFSQSEATEQEFSIASQHYDFL
                     PPLITAFSKCNDNFSYFRIFESTTIRILDEVAQGNSEIGIIYINSQNKKGLLQRLDKL
                     GLEFVELIPFKTHIYLGKDHPLASKTSLIMTDLEGLPTVRFTQDRDDYRYYSENFVEV
                     LDSSVTYNVTDRATLNGILERTQAYATGSGFLDSRSVNGITVIPLEDHLDNQMIYIKR
                     KDRNLSQMALKFVAVMEEYFKGFNS"
     gene            11634..12098
                     /locus_tag="VBE34_000695"
     CDS             11634..12098
                     /locus_tag="VBE34_000695"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEO7263433.1"
                     /translation="MRKIIIPIITILLIALDQLSKLWIVKHIELNQIKEFIPNIVSLT
                     YLRNYGAAFSILQNQQWLFTLITIFVVCVAIIYLMKHINGSYWLLISLTLIISGGLGN
                     FIDRLRLGYVVDMVHLDFINFAIFNVADSYLTIGIICLMIALWKEESNGNHN"
     gene            12082..12972
                     /locus_tag="VBE34_000696"
     CDS             12082..12972
                     /locus_tag="VBE34_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO7263434.1"
                     /translation="MEITIKIAGVRLDKALADNSELSRSQANEEIKKGIVLVNGQVKK
                     AKYTVQEGDRITFDIPKEEVLDYQAENIPLDIIYQDDDVAVVNKPQGMVVHPSAGHSS
                     GTLVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKNDRAHQVLAEELKDKKS
                     LRKYLAIVHGNLPNDRGVIEAPIGRSDKDRKKQAVTAKGKPAITRFHVLERFGDYTLV
                     ELSLETGRTHQIRVHMAYIGHPLAGDPVYGPRKTLGGKGQFLHAQTLGFTHPSNGENL
                     IFSVEVPEIFQTTLDKLRKN"
     gene            13148..13669
                     /gene="pyrR"
                     /locus_tag="VBE34_000697"
     CDS             13148..13669
                     /gene="pyrR"
                     /locus_tag="VBE34_000697"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEO7263435.1"
                     /translation="MKRKEIIDDVTMKRAITRITYEIIERNKNLDNIVLAGIKTRGVF
                     LAKRIQERLKQLENLDIPVGELDTKPFRDDMKVEVDTTTMPVDITDKDIILIDDVLYT
                     GRTIRAAIDNLVSLGRPSRVSLAVLIDRGHRELPIRADYVGKNIPTSQFEEILVEVME
                     HDGYDRVSIIDPS"
     gene            13718..14788
                     /locus_tag="VBE34_000698"
     CDS             13718..14788
                     /locus_tag="VBE34_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEO7263436.1"
                     /translation="MAKKLLILEDGTVFEGLSFGSSLDVTGELVFCTGNTGYQEIITN
                     PSHNGKILVFTSPLIGNYGIHRSYSEAIIPTCLGVVVAEYSRCVSSDTSKMNLDEFLK
                     MKKVPAMSGVDTRYLMQVIKEKGFVKATLAEAGDVLSHLQDQLIATVLPTNNVEQVST
                     KTAYPSPASGRNIVVLDFGLKHSVLRELSKRQCDVTVIPYNTSLEGIKNLYPEGIILS
                     NGPGNPEKLQEVLNTIKELQKSVPMLGIGLGHQLIAMANGAEIMRLPVAKKGPNYPMR
                     DIATGRLETVIQFNHFTVNRLNLPHDLLVTHEGLNDQEIVALRHRSFPVMSVQFYPEA
                     APGPHDVTYFFDEFLEMIGSRA"
     gene            14844..16442
                     /locus_tag="VBE34_000699"
     CDS             14844..16442
                     /locus_tag="VBE34_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEO7263437.1"
                     /translation="MLNTKDKQPILLIIFGQKMKEYDDLIRSTVERLSKRNRPVSILI
                     SKELNHVIHHYDAQFENLETLVGYINTHEDSLIYPLCLDKNHELFQEFLAWQSHYSRQ
                     YISDNLSEKSVQAIDNNQDFVQVDCLVMRDSLNNCLYVSDLEYIESNKTTGKSLAIVP
                     SQTLSDAARQTIRDVAFDVCRKANIIGVCYFSFLIDLNSLDYHIISLSSGLSYQSILF
                     ETITTYPVLEIATKLTVGYTFSQLKHSYYPNTSAFLEPQLDYVATVSFSFEKVDYIFF
                     ARNIEQLFLNLLEASSHDHFPFLSDISEEDLMFALIQKKENRLAYLLEAFRRGFDLYD
                     LSSVTKINPFYLDKCLHIVELYENLNKSQYNVDIYKDAKRYGFSDDYIASSWQISLID
                     MLEYRKKHSVAPVLKQVEQSSGVLTGHQIQYFRSYDWHSDYSSSGCQKALIMVDKGYS
                     LVKLNELIKQIKQTHLELLIVTNQPLLIEQLNDTSIIFDTIGIETILTIMGIEEVEEV
                     YLLGESQADMFSKMKNFSISCQSI"
     gene            16644..17888
                     /locus_tag="VBE34_000700"
     CDS             16644..17888
                     /locus_tag="VBE34_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO7263438.1"
                     /translation="MSKRQNLGISKKGAIISGLSVALIVAVGGFLWVQSQPNKSAVKT
                     NYKVFNVREGSVSSSTLLTGKAKANQEQYVYFDANKGNRATVTVKVGDKITAGQQLVQ
                     YDTTTAQAAYDTANRQLNKVARQINNLKTTGSFPAMESSDQSSSSSQGQGTQSTSGAT
                     NRLQQNYQSQANASYNQQLQDLNDAYADAQAEVNKAQKALNDTVITSDVSGTVVEVNS
                     DIDPASKTSQVLVHVATEGKLQVQGTMSEYDLANVKKDQAVKIKSKVYPDKEWEGKIS
                     YISNYPEAEANNNDSNNGSSAVNYKYKVDITSPLDALKQGFTVSVEVVNGDKHLIVPT
                     SSVINKDNKHFVWVYNDSNRKISKVEVKIGKADAKTQEILSGLKAGQIVVTNPSKTFK
                     DGQKIDNIESIDLKSNKKSEVK"
     gene            17891..18601
                     /locus_tag="VBE34_000701"
     CDS             17891..18601
                     /locus_tag="VBE34_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7263439.1"
                     /translation="MTTNRKELIRLHQIVKSYQNGDQKLQVLKNIDLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDSPTSGDYSLNGKRVEELSQTKLAQVRNKEIGFVFQQFFLL
                     SKLTALQNVELPLIYAGVAPKKRKNLAKQFLDKVELRERMNHLPTELSGGQKQRVAIA
                     RALVNSPSIILADEPTGALDTKTGEQIMQFLTELNQEGKTIIMVTHEPEIADYATRKI
                     VIRDGEITADTTDSIRID"
     gene            18616..19863
                     /locus_tag="VBE34_000702"
     CDS             18616..19863
                     /locus_tag="VBE34_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7263440.1"
                     /translation="MENWKFALSSILGHKMRAFLTMLGIIIGVASVVLIMALGKGMKD
                     SVTNEITKSQKNLQIYYKTKEDQKNEDNFGAQGAFMQGSDTNRKEPIIQESWLKKIAK
                     EVDGVSGYYVTNQTNAPVAYLEKKAKTVNITGINRTYLGIKKFKIKSGRQFQEEDYNQ
                     FSRVILLEEKLAQRLFQTNEAALNKVVTVKNKSYLVVGVYSDPEAGSGLYGSNSDGNA
                     ILTNTQLASEFGAKEAENIYFHLNDVSQSNRIGKEIGKRLTDISHAKDGYYDNFDMTS
                     IVKSINTQVGIMTGVIGAIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRRK
                     ILAQFLIESMVLTILGGLIGLLLAYGGTMLIANAQDKITPSVSLNVAIGSLIFSAFIG
                     IIFGLLPANKASKLNPIDALRYE"
     gene            19992..20264
                     /gene="rpsP"
                     /locus_tag="VBE34_000703"
     CDS             19992..20264
                     /gene="rpsP"
                     /locus_tag="VBE34_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEO7263441.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQVTIKEERVLEWLSKGAQPSDTVRNLLSKAGVMTKFHDQKFSK"
     gene            20274..20516
                     /locus_tag="VBE34_000704"
     CDS             20274..20516
                     /locus_tag="VBE34_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEO7263442.1"
                     /translation="MDTIENLIIAIVKPLISQPDQLTIKIQDGPEFLEYHLDLDTQDI
                     GRVIGKKGRTITAIRSIVYSVPTQGKKVRLIIDEKE"
     gene            20648..22159
                     /locus_tag="VBE34_000705"
     CDS             20648..22159
                     /locus_tag="VBE34_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7263443.1"
                     /translation="MLFDFLEEKIKLKAYLMTALLDDGEINLDHFKTEYALSEKILQG
                     LISELQILYSDFYLVVKSRTMVLFHYETLSKLETLHTIYRESNTLQFLAYFIAPQNVS
                     FSEFTAKKFISVASAYRIKKNCKLYLESVKLSIQKNKVTGPEYQIRLLIALLQYHFGF
                     IIYPFDATSEVMIRDFIEYSNFKGCDYDLKDLSEEYYYFYILVNLVWKRKEYKVEIPQ
                     TESFNQLKKSILYMELSKAAQKSIAIHLGIELNEGLLDYLYLVFCISDGPLFSNRDDI
                     NINEEFIFASYKISKSKAFKNFCGRLLGREFIDSQHFKDINAYFLRHFICYCYYFIPD
                     FYFLNTSRLSYSKDLYHLLDKGLADIFNLKGGNLTFSKHETVLLTMQLSNLIETFLAP
                     LSVYVISSSNIRLQTYQVMLNQYFTSKIAEFFFVNYQTTQIDEKLLKKADIIIAERRY
                     ISSLKNDSGVAIQDILEIGMNNKQYHELLLQAIIEKRDQKVFEYISKMKHKIL"
     gene            22267..22338
                     /locus_tag="VBE34_000706"
     tRNA            22267..22338
                     /locus_tag="VBE34_000706"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22299..22301,aa:Gln,seq:ttg)
     gene            22347..22418
                     /locus_tag="VBE34_000707"
     tRNA            22347..22418
                     /locus_tag="VBE34_000707"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22380..22382,aa:Glu,seq:ttc)
     gene            22481..22999
                     /gene="rimM"
                     /locus_tag="VBE34_000708"
     CDS             22481..22999
                     /gene="rimM"
                     /locus_tag="VBE34_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEO7263444.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVTDFVEERFKKGQVLALFDEK
                     NQFVMDIEIASHRKQKNFDIIKFKGMYHINDIEKYKGFTLKVAEDQLSDLKDGEFYYH
                     EIIGLDVYEGEELIGKIKEILQPGANDVWVVERHGKRDLLLPYIPPVVLEVDLSNQRV
                     QVELMEGLDDED"
     gene            22986..23741
                     /gene="trmD"
                     /locus_tag="VBE34_000709"
     CDS             22986..23741
                     /gene="trmD"
                     /locus_tag="VBE34_000709"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEO7263445.1"
                     /translation="MMKIDILTLFPEMFAPLEHSIVGKAKERGLLEINYHNFRENAEK
                     SRHVDDEPYGGGQGMLLRAQPIFDTIDKIDAQKARVILLDPAGRTFDQDFAEELSKED
                     ELIFICGHYEGYDERIKSLVTDEVSLGDFVLTGGELAAMTMVDATVRLIPEVIGKETS
                     HQDDSFSSGLLEYPQYTRPYDYLGMTVPDVLMSGHHENIRKWRLEQSLRKTLERRPDL
                     LENYAMTDEERLILEKIKTEIERTDTVNEQNNL"
     gene            23722..24714
                     /locus_tag="VBE34_000710"
     CDS             23722..24714
                     /locus_tag="VBE34_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7263446.1"
                     /translation="MNKTIYDITIVGGGPVGLFAAFYAGLRGVSVKIIESLSELGGQP
                     AILYPEKKIYDIPGYPVITGRELIDKHIEQLERFKDSIEICLKEEVLSFEKVDDVFTI
                     QTDKDQHLSRAIVFACGNGAFAPRLLGLENEENYADNNLFYNVTKLEQFAGKHVVICG
                     GGDSAVDWANELDKIAASVAIVHRRDAFRAHEHSVDILKASGVRILTPYVPIGLNGDS
                     QRVSSLVVQKVKGDEVIELPLDNLIVSFGFSTSNKNLRYWNLDYKRSSINVSSLFETT
                     QEGVYAIGDAANYPGKVELIATGYGEAPVAINQAINYIYPDRDNRVVHSTSLIK"
     gene            24851..25921
                     /locus_tag="VBE34_000711"
     CDS             24851..25921
                     /locus_tag="VBE34_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO7263447.1"
                     /translation="MTNTVTPKETAGSFINKVLGGTATAIVVALIPNAILATFLKPFL
                     SYGLAAEFLHIVQVFQFFTPIMAGFLIGQQFKFTPMQQLAVGGAAYIGSGAWAYTEVI
                     QKGVATGSFQLRGIGDLINMMLTAALAVLAVKWFGNKFGSLTIILLPIIIGTGVGYLG
                     WKLLPYVSYVTTLIGQGINSFTTLQPIAMSILIAMAFSMLIVSPISTVAIGLAIGLNG
                     MSASAASMGVASTTAVLVWATMKANKSGVPIAIALGAMKMMMPNFLKHPVMAIPMLMT
                     ATVSSLTVPLFKLVGTPASSGFGLVGAVGPIASFEAGASMLIVILSWLVIPFAVGFVS
                     HKICKDILKLYKDDIFVFEGQN"
     gene            25934..26857
                     /locus_tag="VBE34_000712"
     CDS             25934..26857
                     /locus_tag="VBE34_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEO7263448.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNHDVILLDNWADHIMAIKENGL
                     KVTGDTEDLVKLPIMKPTDATEEADLIILFTKAMQLPNMLQDIKKIIGKKTKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLKGPGHAHLEGVGSVNLQSIDPNNQEAGHRV
                     TELLNEAKLQATYDENVLPNIWRKACVNGTMNSTCALLDCTIGQLFASEDGVNMVHEI
                     IHEFVTVGKAEGVELDEEEITKYVMDTSVKAAHHYPSMHQDLVQNQRLTEIDFLNGAV
                     NKKGENLGIDTPYCRLITQLIHTKENVLSIK"
     gene            27069..28703
                     /locus_tag="VBE34_000713"
     CDS             27069..28703
                     /locus_tag="VBE34_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEO7263449.1"
                     /translation="MMKKGQVNDTKQSYSLRKYKFGLASVILGSFIMVTSPVFADQTT
                     SVQVNNQTGTSVDANNSSNETSASSVITSNNDSVQASDKVVNSQNTATKDITTPLVET
                     KPMVEKTLPEQGNYVYSKETEVKNTPSKSAPVAFYAKKGDKVFYDQVFNKDNVKWISY
                     KSFGGVRRYAAIESLDPSGGSETKAPTPVTNSGSNNQEKIATQGNYTFSHKVEVKNEA
                     KVASPTQFTLDKGDRIFYDQILTIEGNQWLSYKSFNGVRRFVLLGKASSVEKTEDKEK
                     VSPQPQARITKTGRLTISNETTTGFDILITNIKDDNGIAAVKVPVWTEQGGQDDIKWY
                     TAVTTGDGNYKVAVSFADHKNEKGLYNIHLYYQEASGTLVGVTGTKVTVAGTNSSQEP
                     IENGLPKTGVYNIIGSTEVKNEAKISSQTQFTLEKGDKINYDQVLTADGYQWISYKSY
                     SGVRRYIPVKKLTTSSEKAKDEATKPTSYPNLPKTGTYTFTKTVDVKSQPKVSSPVEF
                     NFQKGEKIHYDQVLVVDGHQWISYKSYSGIRRYIEI"
     gene            28847..29932
                     /locus_tag="VBE34_000714"
     CDS             28847..29932
                     /locus_tag="VBE34_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7263450.1"
                     /translation="MTLRELTIEEFKEHSGNYDSQSFLQTPEMAKLLEKRGYDVRYLG
                     YQVENKLEIISLSYIMPVTGGFQMKIDSGPVHSNSKYLKQFYKALQGYAKSNGVLELI
                     VEPFDDYQLFTSSGVPSNQGNDNLIEDFTSSGYHHDGLTTGFTGKYLSWHYVKNLEGV
                     TSETLLSSFSKTGRALVKKAMSFGIKVRVLKRDELHLFKEITTSTSNRRDYMDKSLDY
                     YQDFYDSFEGKAEFVIATLNFREYDHNLQIKAEALENKLKLLDERFRENADSPKYHRQ
                     RSEIINQLASFETRRQEVQSFIQKYDNQDVVLAGSLFVYSLKETVYFFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRGSTFYNLLGI"
     gene            29957..30082
                     /locus_tag="VBE34_000715"
     CDS             29957..30082
                     /locus_tag="VBE34_000715"
                     /inference="COORDINATES: protein motif:HMM:NF014445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEO7263451.1"
                     /translation="MLRFKQNFNGYIIRKMGTFNYYPSPFKYKGIQLLKKVLKRN"
     gene            30147..30956
                     /locus_tag="VBE34_000716"
     CDS             30147..30956
                     /locus_tag="VBE34_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO7263452.1"
                     /translation="MLDIERKRDIIKLQTISIQGGTMLKSKRKEIILSRLEQNKSVTL
                     DELTSILETSESTVRRDLDELESEGFLKRVHGGAELPYSLGQELSNQEKSIKNVQKKL
                     DIARQTAKLIAKQDVIFIDAGTTTELLIDFLPHEQLTVVTNSIHHAAKLVDRGIKTII
                     IGGAVKHSTDASIGQVAINQIRQITVDKAFLGMNGIDEVYLTTPDLEEAAIKEAIINN
                     SQQTFILMDSSKIGQVTFAKVKEINDINLVTNKTDSELMTIIKEKMKVIQV"
     gene            30953..31864
                     /gene="pfkB"
                     /locus_tag="VBE34_000717"
     CDS             30953..31864
                     /gene="pfkB"
                     /locus_tag="VBE34_000717"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEO7263453.1"
                     /translation="MIYTVTLNPSIDFIVRLDTLLLGSVNRMTSDDKYVGGKGINVSR
                     ILKRLKIDNTATGFIGGFTGHFVEDGLVSEGIKTDFVSVNEDTRINVKVKAKIETEIN
                     GGGPRITNEQLHRLEKLLSRLTPEDTVVFAGSAPASLGNKVYNTLIPIAKKTGAEVVC
                     DFEGQTLLDALAYQPLLVKPNNHELADIFGVELEGLPDIEKYAHKILDKGAKNVIVSM
                     AGDGALLVTPEASYFAKPIKGEVKNSVGAGDSMVAGFTGEFVKSKNPVEALKWGVACG
                     TATTFSDDLATAEFIQDIYNKVEVEKL"
     gene            31861..33825
                     /locus_tag="VBE34_000718"
     CDS             31861..33825
                     /locus_tag="VBE34_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7263454.1"
                     /translation="MKIQDLLKKEVMIMDLKATSKEAAIDEMITKLVDTGVVTNFAIF
                     KDGIMKREAQTSTGLGDGIAMPHSKNAAVKEATVLFAKSASGVDYEALDGQPTDLFFM
                     IAAPDGANDTHLAALAELSKYLLKEGFADQLRQAKTPDDIIATFDSNSISQETVAPQT
                     VQSTSKGSDYIVAVTACTTGIAHTYMAEEALKKKAAEMGVGIKVETNGASGVGNKLTS
                     SDIARAKGVIIAADKAVEMDRFDGKPLVSRPVADGIKKSEDLINIILDNKAQTYHAKN
                     QNDKQSGESDGKSGLGSAFYKHLMGGVSQMLPFVIGGGIMIAIAFLFDNILGVPKDQL
                     SNLGSYHEIAALFKNIGGAAFAFMLPVLAGYIAYSIAEKPGLVAGFVAGSIASSGLAF
                     GKVPFAEGGKATLALAGVPSGFLGALVGGFLAGGVILLLRKLLSGLPKSLEGIKSILL
                     YPLLGVLITGFLMLLVNIPMAAINTALNTFLQGLSGSSAVLMGLLVGGMMAVDMGGPV
                     NKAAYVFGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFNNEERQSGL
                     TNIVMGLSFITEGAIPFGAADPARAIPSFIVGSALTGALVGLAGIKLMAPHGGIFVIA
                     LTSNPLLYILFILIGAIVSGVLFGLFRKTK"
     gene            34014..>34284
                     /locus_tag="VBE34_000719"
     CDS             34014..>34284
                     /locus_tag="VBE34_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEO7263455.1"
                     /translation="MIKWNEKETKLVDTSFSIMLYLKTLFAVGQVVAGVLLYYFKPTQ
                     IKHLLFLLTEGEVHGGIHGFISAHFLKISSYINDSASHLAAIYLLI"
BASE COUNT        11558 a         5026 c         6799 g        10901 t
ORIGIN      
        1 taaagaacaa caagaaaaaa ttggttattt aggctttata tatcctaata aaaagaacaa
       61 taagggtagg aaagatattt ttattttgac aaactctgac caaattgaac atttagaagt
      121 gttggttcat cacttgtctg attatcattt tcatattgca gcgtacacag aaatgtcttt
      181 taagttgatg tcatttagcc aagaacaaaa tgtaacgcta tatccgaata tttcaagaac
      241 tgatttagac aacttatttg aaatctgtga tatttatttt gacattaatc atggaaatga
      301 agttgatgat gttattagaa gggcatttga atataatcac cttatctttg cttttgataa
      361 tacctgtcat aacagagagt tagtattaga tagcaatatc atttctcaca caacctgtga
      421 acaattgata aatttaatga aaaatttatc aggctccatt atgtatttgc tagagcaaca
      481 aagagaacaa acaagtaatg aaacaaaaga gcgttataaa gaaatattag gagggtatgg
      541 aaatgcctaa aagaaatgaa ttactcaata aagaaattaa aatgagtatt gataaactta
      601 gatataaaga accagagagt gaacatgaca agcgacctac tttttatttg gtagtactta
      661 tacttgttac tgtagcagtt atattgtcgt tatttaaata ttttttatag attcctatta
      721 atggtagaaa agaagcctgt tgttttttag ataggggaag tgtgtgagga catatattac
      781 aaacttgaat ggacattcaa tcactagtac agcacaaata gctcaaaaca tggtaacaga
      841 tatagcagta agcttaggtt ttcgtgagct gggaatacat tcttatccga ttgatactga
      901 ttctcctgag gaaatgagta agcgtttaga tggaatctgt tccggactta gaaaaaatga
      961 tattgtcata tttcagacac ctacatggaa cactacaact tttgatgaaa aattatttca
     1021 caaattaaaa atatttggtg taaagattgt tatttttata catgatgttg taccgctaat
     1081 gtttgatgga aatttttatt tgatggatag aactatagct tattataatg aagcagatgt
     1141 tttaatagcc cctagtcaag caatggtcga taagcttcaa agttatggct tgacagtaaa
     1201 aaaaatctta gttcaaggaa tgtgggatca tcccaccaat attactttac aagcagttaa
     1261 tcataagaag cttgttcatt ttcctgggaa cccagagcgg tttaacttca taaagaattg
     1321 gagaattcct accgagttac atgtttatac tgaccataac atgcaattac ccaccactgt
     1381 ggttaaagag ccatatcaat cggacgaaca attaattatg aaaatgagcg aagggggata
     1441 tggcttagtc tggatggatg accgtgataa acaatatcaa tctctttatt gcccttacaa
     1501 actgggcgct tatatagcag caggaattcc agtgattatt caaaaaggta ttgctaatca
     1561 agatataata gaaaaaaata atttgggatt tattattgaa aaaattgatg atattagtaa
     1621 tatagttgaa agtactactg aagaggaata tatggaaatt gttagcgatg tcaggcggtt
     1681 taatccttta gttagacaag gatattttac gcgaaaacta ttaaccgatg cagtatttag
     1741 tgcattaaat agcatgtgag gggaatatga aagaactaga agaaatcgta gttttcccta
     1801 taaacaaaag tttggattac attttggaaa atcattcgtc agttattcgc tttggggacg
     1861 gtgaatttga cattataatg ggaaaatcga ttccttatca agattacaac ccagagttag
     1921 cagataggtt attgcaactt cttcataagc aaagtacgaa tgatctatta gtatgtcttc
     1981 cagatatgtt tcaaaatcga caaagatata atcaatcggc acaacttttt tggaaaaatc
     2041 attttcaaaa atttggcaat ttctataggc aacattgtta tcaagattgg tatggttctt
     2101 cttttatttc aaggccatat atggacttac aagataagga gtctgctgta gaatcattta
     2161 aaaaattaaa gttactttgg gacaaacgcg atattttggt agttgaaggg gaaacttcgc
     2221 ggtcgggtgt aggtaatgat ctttttaata attgcaatag tttaaaaagg attatttgtc
     2281 cttctaaaaa tgcttatata aaatatgatg aaattcttga ggctgttgaa agatatgctc
     2341 aagggaggat aatcttattg atgctaggtc cgactgcaaa aattttagta agagatttat
     2401 ctgatagagg atttcaagcc attgatatcg gtcatattgt ttctgaatat gaatggtata
     2461 agatgggagc tactcaaaaa ataaaatttt ctcataaaca caccgctgag tttaacgatg
     2521 atgaaaatat taatttaatt gacagtcaga attatattga tgagattgtt aaaacaataa
     2581 ctatcgatga tactgaattc agggatactg aattaatatc aatcatactt cctgtctata
     2641 atatgtctgg ttctttatca aggtgtttag attctatcct taggcaagtt tactcaactt
     2701 ttgaggtgat cttagtcaac catggttcta cagatggaag tggtaatatt tgcgagaaat
     2761 atgcgatgaa ggatgagcgt attcgtttat ttcatgaaga acatcaagtt attacatctg
     2821 tatggaactt cgcattagag aaatctaaag ggaagtatgt tacttttgtg aatccagatg
     2881 attttcttga tgagtcttac ttaaattatc tttacaataa attaagagct aataaatctg
     2941 atatctcagc aaccacctat acttttttta atgaagagga aaaatgcttt gtttattttg
     3001 caacagatga taattatttt gagaaaacat tctttgctca agaatggtta gagcaagaac
     3061 atagttacca cgataattta aaacaaatat ttcctctcct ttctatgaag ctctttagaa
     3121 aatcactttt taacaatatc acttttcctt tagaagattt aacaggagaa tacacactgt
     3181 ataatgttta tttgctatca aggcacatta cattttctca tattggttta tacataacct
     3241 ctcaagtttt ctctcatact gaaataaaaa gattcacggg acatgatata gagaagatgc
     3301 tatctagaaa ggaagagagg ttagccctgt taacaatggt agggtttaat tctgagaaat
     3361 acatcaaaaa ttataaggaa actttattaa attatcaaag tgagtcgttg aatataggtg
     3421 atattaattt atataataaa attaagcaaa agattgcatt gatagattat tgttactaga
     3481 aataatttta acagcaagtt aggatatgat aatcggtaat ataaattgtt tacgtagcag
     3541 aaaattgtga ctgtaaacac tttgtgatag agtataggta tcaatgattc aaggagagag
     3601 taatgtcaaa acaatatgat tatatcgtta ttggtggagg tagtgcaggc agtggtaccg
     3661 ctaatagggc agccatgtat ggagcaaaag tcctgttaat tgaaggtgga caagtaggtg
     3721 gaacttgtgt taacttaggt tgtgtaccta agaaaatcat gtggtatggt gcacaagttt
     3781 ctgagacact ccataagtat agttcaggtt atggttttga agccaataat cttagttttg
     3841 attttactac tctaaaagct aatcgcgatg cttacgtgca gcggtctaga cagtcgtatg
     3901 ccgctaattt tgagcgtaat ggggtcgaaa agattgatgg atttgctcgt tttattgata
     3961 accatactat tgaagtgaat ggtcagcaat ataaagctcc tcacattact attgcaacag
     4021 gtggacaccc tctttaccct gatattattg gaagtgaact tggtgagact tctgatgatt
     4081 tttttggatg ggagacctta ccaaattcta tattgattgt tggggcgggc tatatcgcgg
     4141 cagaacttgc tggagtggtt aatgaattag gcgttgaaac ccatcttgca tttagaaaag
     4201 accatattct acgcggattt gatgacatgg taacaagtga ggttatggct gaaatggaga
     4261 aatcaggtat ctctttacat gctaaccatg tacctaaatc tcttaaacgc gatgaaggtg
     4321 gcaagttgat ttttgaagct gaaaatggga aaacgcttgt cgttgatcgt gtaatatggg
     4381 ctatcggccg tggaccaaat gtagacatgg gacttgaaaa taccgatatt gttttaaatg
     4441 ataaagatta tatcaaaaca gatgaatttg agaatacttc tgtagatggc gtgtatgcta
     4501 ttggagatgt taatgggaaa attgccttga caccggtagc aattgcagca ggtcgtcgct
     4561 tatcagaaag actttttaat cataaagata acgaaaaatt agattaccat aatgtacctt
     4621 cagttatttt tactcaccct gtaattggga cggtaggact ttcagaagca gcagctatcg
     4681 agcaatttgg aaaagataat atcaaagtct atacatcaac ttttacctct atgtatacgg
     4741 ctgttaccag taatcgccaa gcagttaaga tgaagctcat aaccctagga aaagaggaaa
     4801 aagttattgg gcttcatggt gttggttatg gtattgatga aatgattcaa ggtttttcag
     4861 ttgctatcaa aatgggggct actaaagcag actttgatga tactgttgct attcacccaa
     4921 ctggatctga ggaatttgtt acaatgcgct aacgctatca aagaatacaa aaaaagctag
     4981 ggaacctagc ttttttaagg tgttgttgta ttttcaatag ttgaggtatc ggtagtagta
     5041 ttatttgttt gatcattgtt agtactagat gatgtttgat cgttattggc agcagaattt
     5101 gaactgctac tcggagtact actagcagag gaattttcta gtgtttggct ggatgatgcc
     5161 cttgtctcat ctttttcagt agaaacttgg ttactagaat tttgtccact ttctcttata
     5221 atgacggttt ttgaagtaga agaatcagtc ttactagagt ctttgtgatt attaatcgca
     5281 ttagtaatag taatagaggc aacgataata cttaaaacac taccgactaa tgcaattgtt
     5341 ttttgaagtg cagaaaaacc tgtttttata tgcttagcgg gtgattgagt tgaatcagta
     5401 ttagtcttag tagacttttt ctgttgtctt gaatatcctt ttgacatgac gccctcctta
     5461 aatgtacatc aactattata tgattttatc ctaaaaaaag ctaaagaaaa aataatttaa
     5521 aacatttaaa taagcaacct aggttattaa gctaagctat gttagtgtaa taggtagtga
     5581 gttttgtgat aaattatgag aattatcata aatattgttt tgggatgttc acaaactaat
     5641 ataaatagta ggaaaattat gctataataa cactgattga ctttagaagg acagatttat
     5701 gatttatttt gataattcgg caactactat tccctatcca gaagttttaa ggacttatca
     5761 agaagtagct agtaaaatct atggaaaccc ctcaagttta catgagctag gaacaacatc
     5821 ttctcggatt ttagaggcta gccgtaaaca aattgctagt ttacttgagc ttaaagcaaa
     5881 tgaaattttc tttacctctg gtggtacaga ggccgataat tgggttatta agggtcttgc
     5941 gtttgagaaa caacattttg gaaatcatat tattgtttct gatatcgaac acccagctgt
     6001 taaagagtct gctaaatggc ttggagagta tgggtttgag attgattatg cgccagtaga
     6061 tgataaagga tttgtagacg tagaagcact ggtcaaatta attaaaccag aaacgattct
     6121 tatttcaatt atggcaataa acaatgaaat tggttctatt cagccaataa aagctatttc
     6181 agacttgttg tcagataaac ctactatatc gtttcatgtt gatgctgttc aagcaatagg
     6241 taagataccg actaaagatt atttgactga gcgagttgat tttgcatctt tttctagtca
     6301 taaatttcac ggcgttcgtg gggttggctt tctatatatt aaagaaggaa aacgtatttc
     6361 accattacta acaggtggtg gacaagaatc tgatttacgt tcaactactg agaatgtcgc
     6421 agggattgca gctactgcta aagctttacg aatggttatg gacaaggaag tagtagctat
     6481 tcctaaaata agtaaaatga aaaccattat tcatgatgaa ttagccaaat atgaggatat
     6541 tacgttattt tcgggtaagg aaggtttttc acctaacatt atcacgtttg gtatcaaggg
     6601 tgtccgagga gaagttcttg tccatgcctt tgagggacat gatattttta tctcgacaac
     6661 tagcgcttgc tcttctaaag caggtaaacc agcaggaact cttatagcaa tgggaatttc
     6721 aactaagtta gcccaaactg ctgtacgtat cagcttagat gatgataatg atatgggaca
     6781 agtagagcaa tttttaacta tttttaaaca agtttatgaa aaaactaaga aagttagagg
     6841 ataagatgca gtattcagaa attatgattc gttatggaga actctctact aagaagaaaa
     6901 accgtatgcg cttcatcaat aagttaaaaa ataatatgga gcatgtactc tccatttatc
     6961 cagatgtttc agtaaaaaca gatcgtgata gaggacatgt atatctcaat ggtacagatt
     7021 atcatgaagt tgcagagtcc ttaaaagaga tttttggtat ccaagctttt tctccatctt
     7081 ttaaagtaga aaaaaatgtt gatacattgg taaaagctgt ccaggaaatt atgacttccg
     7141 tttataaaga tgggatgact tttaaaatta ccgcaaaacg tagtgaccac tcatttgaat
     7201 tggatagccg tgctctaaat catactttag gagatgccgt tttttcagtc ttgccaaata
     7261 ttaaggctca gatgaagcaa ccagatatca atcttaaagt cgagatacga gatgaggctg
     7321 cttatatttc atatgaggat attaggggtg caggaggatt accggtagga acatctggga
     7381 aagggatgct gatgttgtct ggtgggattg attctccggt agcaggttac ctagcgttaa
     7441 aacgtggtgt agatatagaa gcagtccatt ttgcaagtcc tccttatact agcccaggtg
     7501 cattgaaaaa agcacatgat ttaacacgta aattgacaaa atttggtggt aatattcaat
     7561 ttattgaagt tccattcaca gaaattcaag aggaaattaa ggaaaaagct cctgaagcct
     7621 acttgatgac gttaacacgt aggtttatga tgcgtattac agatcgtatt cgtgaggacc
     7681 gaaatggtct tgttattatt aacggtgaaa gtttagggca ggtggcaagc caaacgctag
     7741 aaagtatgca agctattaat gctgtcactg caacaccgat tattcgtcct gtggtcacga
     7801 tggataagct agaaattatt gatattgctc aaaaaataga tacttttgat atttcaattc
     7861 aaccatttga ggattgctgt acaatttttg caccagatcg cccaaaaact aaccctaaaa
     7921 ttaagaatac agaacagtat gagaaacgta tggatgtaga aggtctggta gagagggcag
     7981 ttgcagggat tatggtaact actattcaac ctcaagcaga tagtgatgat gttgatgact
     8041 tgattgacga tttattataa tgaattttta aactacagat ttactctgta gttttttatt
     8101 aaaatgattg tgctgtcata aggttataaa aaatgctata atacgaataa aggaggcaag
     8161 tatggcaaga atcccttatc aaaaaacact tattttttgt gtagccgtta ttattgctat
     8221 ttttattttg gggttatcta aggatttagc acaatctaaa gggcaaaagg ttgcaaataa
     8281 taatactgta aaaacagcac gtgtggttgc aaacggagat attttgctgc acgatgtact
     8341 atatgccagt gcgcgccaac ccgacggcac ttataatttt actccttatt ttaaagaggt
     8401 aaaatcttgg attgagagcg ctgatttagc gattggtgat tatgaaggga cgattagttc
     8461 agaatatcct ttagcgggct atcctttatt taatgctcct aatgagattg caactactat
     8521 gaaagaaact ggatatgatg tcgttgactt agcacataat catattctgg attcacaact
     8581 ggcaggtgct atcaatactg tcaagacttt taatcgtctg ggattggata ctattggggt
     8641 ttatttaaag gatagaaata aagaagatat cttaattaag cgtgtaaatg gtattaaaat
     8701 tgctatttta ggttattcgt atggctacaa tggtatggaa gctaatgttt caaagagtga
     8761 ctatgaaaaa catatgtctg atttagatac taaaaagatt aagcaagata ttaaaaaggc
     8821 tgaaaaagaa gcagatatca ccattgtcat gccacagatg gggatagaat atcaaaaaaa
     8881 acctactaca gaacaagtaa tactttatca tagtatgatt aaatgggggg cagatattat
     8941 attcggagga caccctcacg ttgttgaacc gtctgaagtg ataaaaaaag atggccagaa
     9001 aaaatttatt atttactcta tgggaaattt tatttcaaat cagcgtttag aaacggttga
     9061 tgatatctgg acagaaagag gattattaat ggatgtaacc atagagaaaa aaggtcaaaa
     9121 aacagtaatt aaaaaagtta aggcgcatcc aacactagta gaagcaaaag caaatggccg
     9181 ttatgttgaa gaaggctttc cagcttatga ttataggacg ctagttttag aggattatat
     9241 tgaaggtggt aagaaacgtt ctacaattga tcagactatg aaagataaag ttgataaagc
     9301 atataaggaa atgaatgagt tagttaattt gaagtggtaa agttgataat tttattttgt
     9361 tttatcttgc ataaaaaaag agtttatgat aaaatttgaa tgttgactaa atgcacatcc
     9421 tgtgcaaccg cgcgaaaatc gttagattaa gtagggcaag gctctcacga tttataggcg
     9481 agtcttcaca gagggaaacc tcacaagaaa tttttatagg aggtgcatca tgagcacata
     9541 cgcaatcatc aaaactggtg gtaaacaagt taaagttgaa gtaggacaag ctatctacgt
     9601 tgaaaaactt gacgttgaag caggagcaga agttacattt aacgaagttg ttcttgttgg
     9661 tggtgaaaca actaaagttg gtactccagt tgttgaagga gctactgttg ttggtactgt
     9721 tgaaaaacaa ggcaaacaaa agaaagttgt ttcttacaaa tacaaaccta aaaaaggtag
     9781 ccaccgtaaa caaggtcacc gtcaacctta tacaaaagtt gttatcaacg caatcaacgc
     9841 ttaatttttc atgattaaag caacttttac tcgtaatcaa tcaggctatc tttatagtgc
     9901 tgaaatcagc ggtcatgctg gtagtggcga gtacggcttt gatgtgattt gtgcagctgt
     9961 tagtacttta tcaattaatt ttattaattc attagaggct ttaacaacct gccaagcgca
    10021 attgattatc aatgacgttg aaggtggtta tatgaagata gatttatctt ctataccaca
    10081 acataaagag gataaggttc aattattatt tgagtcttac cttcttggaa tgactaatct
    10141 gtctaaagat tcttctgaat ttgttagtac agtagtcatg acacactaat ttaagagagg
    10201 acacaacatt atgttaaaaa tgaatcttgc taacttgcaa cttttcgccc acaaaaaagg
    10261 tggaggttct acatcaaacg gacgtgattc acaagcaaaa cgtcttggag ctaaagcagc
    10321 tgacggacaa acagtatcag gtggatcaat tctttaccgt caacgcggta cacacatcta
    10381 tccaggtgct aacgttggac gtggtgggga cgatacactt tttgctaaag ttgaaggtgt
    10441 tgttcgtttc gaacgtaaag gacgcgataa aaaacaagtg tctgtttacc caatcgcaaa
    10501 ataatgccta gttaagactt tatgactatt cataaggtct tttattattg tcagagcttt
    10561 cattttgtaa caaaagacaa tatgttaaga gatttatcat taatactcat aaactttgtt
    10621 gttcttttat agtgaaatag tcctaattca tgaagaattt tccgcaagga tagttatttt
    10681 ttggtataat gaagggtact aaaatgagga agtgataaaa tgaatattca acaactgcgt
    10741 tatgttgttg ctatcgcaaa tagtggtact tttcgtgaag cagctgcaaa actatttgtt
    10801 agtcagccaa gtttatctgt tgccgttcgt gatttggaaa cagaattagg gtttcagatt
    10861 tttacgcgta caactactgg tgcagtgttg accaatcaag gcatgacttt ttatgagaat
    10921 gctttagaag ttgtgaaaag ctttgattca ttcgaaaaac agttttcgca atctgaagct
    10981 acagagcaag aattctcaat agcaagtcaa cattatgatt ttctacctcc tttaatcaca
    11041 gcgttttcaa aatgtaatga taattttagc tatttccgta ttttcgaatc aacaacaata
    11101 cgtatcttag atgaagttgc tcaagggaat agcgaaatag gtatcattta tatcaatagt
    11161 caaaataaaa aaggtctact tcagcggttg gacaaattgg ggttagagtt tgtagagctt
    11221 attcctttta aaactcatat ttatcttggg aaagaccatc ccttggctag taaaacatct
    11281 ctgataatga cagatttaga aggattacca accgtacgtt ttacacagga tagagatgat
    11341 tatcggtatt actcggagaa ttttgtggaa gtacttgata gttctgttac ctataatgtt
    11401 actgatagag caacattaaa tggtatttta gaaagaaccc aagcttatgc gacgggctca
    11461 ggattcttgg atagccgcag tgtgaatggt atcactgtta ttccattaga agatcatcta
    11521 gataaccaaa tgatttatat caaacgaaaa gatcgtaatt tatctcagat ggctttaaaa
    11581 tttgttgctg ttatggaaga gtattttaag ggatttaatt cataggagaa gtaatgagaa
    11641 agattattat accaataata actattttgt tgattgcttt agatcaacta tctaagttat
    11701 ggattgttaa acatattgaa ctaaaccaaa ttaaggaatt cattccaaat atcgttagtc
    11761 taacttatct gagaaattat ggtgctgcat tttctatttt gcaaaatcaa caatggcttt
    11821 ttaccttaat tacaatcttt gttgtttgtg ttgctattat ttatttgatg aaacatatta
    11881 atggaagtta ttggttatta atttctctaa ccttaatcat ctctggtgga ttaggaaact
    11941 ttatagatcg cttaagatta ggttatgtcg ttgatatggt gcatttagat tttattaatt
    12001 ttgctatctt taatgtagca gactcatatc tgacaatcgg tattatttgc ttaatgattg
    12061 cactttggaa agaggaaagt aatggaaatc acaattaaaa tagcaggagt taggctagat
    12121 aaagctttag ctgataattc tgaactgtcg cgtagtcaag ctaatgaaga aattaaaaaa
    12181 gggattgtgt tagtaaatgg acaagtaaaa aaagcgaaat atactgttca agaaggagat
    12241 cggattactt ttgatattcc taaagaggaa gtactagatt atcaagctga aaatatccct
    12301 ttagatatta tctatcaaga tgatgacgtt gctgtggtta ataagccgca agggatggtt
    12361 gttcaccctt ctgcaggcca cagttcgggc accttagtta acgccttgat gtatcatatt
    12421 aaggatttat catcaattaa tggcgttgtt cgcccaggga tcgttcatcg tattgataaa
    12481 gatacttctg gtttattgat ggttgcaaaa aatgatagag ctcaccaagt gttggctgaa
    12541 gagttgaaag ataaaaaatc gcttcgtaaa tatttagcaa ttgtgcatgg taatttacca
    12601 aatgatcgag gcgtgattga ggctccgatt ggtagaagtg ataaagatcg taagaagcaa
    12661 gccgtgactg ctaaaggtaa gccagcaata actcgttttc atgttttaga acgttttggt
    12721 gattatacct tagttgaatt gtcattggag acaggtcgaa cgcaccaaat tcgggttcat
    12781 atggcttata ttggccatcc tctagccggt gatcctgtct acggtccccg caagacttta
    12841 gggggtaaag gacagttttt gcatgcacaa acactaggtt ttacgcaccc tagtaacgga
    12901 gagaatttga ttttttcagt agaggttccg gaaatttttc agacaacttt agacaagttg
    12961 agaaaaaatt aagtttttct tgaaaaatac ttttaccatt tgctataata taagcaaatc
    13021 attcctttaa ccttatccag tgagatagtt aaggagaaac ttcagactaa atcaatgcct
    13081 gagcggcttt gtcattctag agagtctcct tttgaagggg ctcttttatc ttagagagga
    13141 tactgcaatg aaaagaaaag aaattattga tgatgtgacc atgaaacgtg ctattacacg
    13201 tattacctac gaaattatag aacgtaacaa aaatttagat aatattgttt tagcaggcat
    13261 taaaacaaga ggtgtttttt tagccaaacg cattcaagaa cgtttgaagc aattagaaaa
    13321 tttagatatt cccgttggag aattggatac taaacctttc cgtgatgata tgaaagttga
    13381 agttgacacc acaactatgc cagttgacat cactgataaa gatattatat taattgatga
    13441 tgttctctat acaggtcgca ctattcgagc tgctattgat aatcttgtta gtttggggcg
    13501 tccgagtcgt gtcagtttag ctgtattaat tgaccgtggg catcgtgaac tacccattcg
    13561 tgcagattat gtggggaaaa atattccaac aagtcaattt gaagaaattc tagttgaagt
    13621 gatggaacat gatggttacg atcgtgtgag tattattgat ccgagttaag tagacgacta
    13681 ttatttacga tgattttata gaaggggaag aaccacaatg gcaaaaaaat tgttaattct
    13741 agaggatggt acggtttttg aaggactatc ctttggttca tctcttgatg taactggcga
    13801 attagttttt tgtacgggga ataccggcta tcaggaaata ataacgaatc cttcacataa
    13861 tggaaaaatt ttagttttta cctcaccgct aataggaaat tatggtattc atcgtagtta
    13921 ctctgaagcc attattccca cttgtttagg agttgttgtt gcagagtatt ccagatgtgt
    13981 aagtagcgat actagcaaga tgaatctaga tgaatttttg aaaatgaaaa aggttcctgc
    14041 aatgtcgggg gtcgatacaa ggtatttgat gcaagttatt aaagaaaaag gatttgtaaa
    14101 agctacttta gcagaagcag gtgatgtttt atcacattta caagatcaat taatagcgac
    14161 agtattaccg acaaataatg tggaacaagt ttcgacaaaa acagcttatc cgtcgcctgc
    14221 tagcggtcga aatattgttg tacttgattt tggccttaaa cattctgttt tgagagaatt
    14281 gtctaaacgt cagtgtgacg tgactgtcat tccctacaat acgtctttag aaggtatcaa
    14341 aaatctatac ccagaaggta tcatattatc taatggtccg ggaaatccag aaaagcttca
    14401 ggaagtctta aacactatta aagaattaca aaaatctgtt cctatgttag gtattggttt
    14461 ggggcatcaa ttaattgcca tggcaaatgg tgcagaaatt atgagactcc cagttgctaa
    14521 gaaggggcca aattatccca tgagagatat tgctacgggg cgtttagaaa cagttattca
    14581 gtttaatcat tttaccgtta accggctaaa tcttccgcat gatttactgg taacacacga
    14641 aggtttgaat gatcaagaga ttgttgctct aaggcaccgc tcatttccag tgatgtccgt
    14701 gcaattttac ccagaggctg ctccaggccc tcatgatgtg acctattttt ttgatgaatt
    14761 tttagaaatg attggatcac gcgcttaaac ataaggtatt atttaaagtc agtcggtgag
    14821 ctgattaata aggaggtgcg tatatgctaa atacaaagga taaacagcct attttgctta
    14881 ttattttcgg acagaaaatg aaagaatatg acgatttaat tagatcaact gttgagaggt
    14941 tatctaaaag gaatagacct gttagtattc ttatctcgaa ggagctaaac catgtgattc
    15001 accattatga tgcacaattt gaaaatcttg agacgcttgt ggggtatata aatacacatg
    15061 aagatagttt gatttatccc ctttgcttag ataaaaatca tgagctattt caagagtttt
    15121 tagcgtggca gagtcattat tcaaggcagt atatcagtga taatttatca gaaaaaagtg
    15181 ttcaggcaat agacaataat caagactttg tgcaagtaga ttgtttagta atgagagact
    15241 ctttgaataa ttgtttatat gtctctgatt tagaatatat tgaatctaat aaaacaactg
    15301 gtaagtctct agcgattgta ccaagtcaaa ctttatccga tgctgctagg caaacaattc
    15361 gagatgttgc atttgatgtg tgtcgaaagg caaacattat tggagtatgt tactttagtt
    15421 tcttaataga tttgaacagt ttggattatc atataattag cttatcatct ggtttgtctt
    15481 atcaaagcat tttatttgag actattacga cctatcctgt tttagagata gcaactaaat
    15541 tgacagtggg atatactttt agtcaactga agcattctta ttacccaaat acctctgcct
    15601 ttttagagcc gcaattagac tatgtagcta ccgtatcttt tagttttgaa aaagttgact
    15661 atatcttttt tgctcgtaat atagagcaat tatttttaaa tttactcgag gctagcagtc
    15721 atgatcattt tccatttctg agtgatatta gcgaggaaga tttgatgttt gctttgattc
    15781 agaaaaaaga aaatcgtcta gcttatcttt tagaggcttt tagacgtggc tttgatttgt
    15841 atgatttatc ctcagttact aaaatcaacc ctttttactt agataaatgt ttacatattg
    15901 tagagttgta tgaaaacctg aataaaagcc aatataatgt agatatttac aaggatgcta
    15961 aaaggtatgg tttttcggat gactatattg ctagtagttg gcaaatatct ttgatagaca
    16021 tgttagaata tcgaaaaaaa catagtgtcg cacctgttct gaagcaagtc gaacaaagtt
    16081 ctggtgttct aactggccat caaattcagt attttcgaag ctatgattgg cattcagact
    16141 acagttctag cggttgtcaa aaggctttga taatggtaga caagggttat tccctagtaa
    16201 aattaaatga gttaattaag caaatcaaac aaactcattt agaattatta attgttacca
    16261 atcaacctct cctcatagaa caattaaatg atacatctat aatttttgac actataggta
    16321 ttgagaccat attaacaatt atgggtatag aagaggtgga agaagtgtat ttattgggag
    16381 aaagtcaagc agacatgttt tcaaaaatga aaaatttttc tatctcttgt cagtctattt
    16441 agcaattctt aataaaagct gagatataga tctcagcttt tatttgtggt aaaaagtaaa
    16501 gtactttcaa ttttgtaagt tctttctatg agcaaaatag tatacttaat gagtaaaaat
    16561 attattttta aagaattttc ttgacaatat tatttaaaaa taatacactt gttctatatt
    16621 aataatacaa acaggagatg gacatgagta aacgacaaaa tttagggatt agtaaaaaag
    16681 gagcaattat atcagggctc tcagtggcac taattgtagc agtaggtggc tttttatggg
    16741 tacaatctca acctaataag agtgcagtaa aaactaatta caaagttttt aatgttagag
    16801 aaggaagtgt ttcgtcctca actcttttga caggaaaagc taaggctaat caagaacagt
    16861 atgtgtattt tgatgctaat aaaggtaatc gagcaactgt tacagttaaa gtgggtgata
    16921 aaatcacagc tggtcagcag ttagttcaat atgatacaac aactgcacaa gcagcctacg
    16981 acactgctaa tcgtcaatta aataaagtag cgcgtcagat taataatcta aagacaacag
    17041 ggagttttcc agctatggaa tcaagtgatc aatcttcatc atcatcacaa ggacaaggga
    17101 ctcaatcgac tagtggtgcg acgaatcgtc tacagcaaaa ttatcaaagt caagctaatg
    17161 cttcatacaa ccaacaactt caagatttga atgatgctta tgcagatgca caggcagaag
    17221 taaataaagc acaaaaagca ttgaatgata ctgttattac aagtgacgta tcagggacag
    17281 ttgttgaagt taatagtgat attgatccag cttcaaaaac tagtcaagta cttgtccatg
    17341 tagcaactga aggtaaactc caagtacaag gaacgatgag tgagtatgat ttggctaatg
    17401 ttaaaaaaga tcaggctgtt aaaataaaat ctaaggtcta tcctgacaag gaatgggaag
    17461 gtaaaatttc atatatctca aattatccag aagcagaagc aaacaacaat gactctaata
    17521 acggctctag tgctgtaaat tataaatata aagtagatat tactagccct ctcgatgcat
    17581 taaaacaagg ttttaccgta tcagttgaag tagttaatgg agataagcac cttattgtcc
    17641 ctacaagttc tgtgataaac aaagataata aacactttgt ttgggtatac aatgattcta
    17701 atcgtaaaat ttccaaagtt gaagtcaaaa ttggtaaagc tgatgctaag acacaagaaa
    17761 ttttatcagg tttgaaagca ggacaaatcg tggttactaa tccaagcaaa actttcaagg
    17821 atgggcaaaa aattgataat attgaatcaa tcgatcttaa gtctaataag aaatcagagg
    17881 tgaaataatc gtgacaacta atcgaaaaga actgattaga ttgcatcaga ttgttaaaag
    17941 ttatcaaaat ggggatcaaa aacttcaagt cttaaagaac attgatttga cggtttatga
    18001 aggtgaattt cttgcaatca tggggccatc tggctcaggg aaatccacac taatgaatat
    18061 tattggcttg ttggattccc caacttcagg agattatagt ttaaatggta aacgtgtcga
    18121 agaactaagt caaacaaaat tagctcaagt tcgaaataag gaaatcggtt ttgtctttca
    18181 acaatttttc ttgttatcta aactaacggc tttgcaaaat gttgaattac ccttaattta
    18241 tgcgggtgta gctcctaaaa agcgtaaaaa tttagcaaaa caatttttag ataaagttga
    18301 attgagagag cgtatgaacc atctgccaac agaattatct gggggacaga agcaacgtgt
    18361 agcaatcgcg cgtgctttgg tgaatagccc atctattatt ctagcagatg agccgactgg
    18421 tgccttggat acaaagacgg gtgagcaaat tatgcagttc ctaacggaat taaatcaaga
    18481 aggtaaaacc attatcatgg taactcatga accagaaatc gccgattatg cgacacgtaa
    18541 aattgttatt cgtgatggcg aaattacagc agatactact gatagtatta gaattgatta
    18601 aggggagatt cgattatgga aaattggaaa tttgccctat catcgattct agggcacaag
    18661 atgcgtgctt ttctgaccat gttagggatt atcattggag ttgcatcagt tgtgttaatt
    18721 atggccttag gtaagggcat gaaagattcg gttacaaatg agattacaaa gtctcagaag
    18781 aatcttcaaa tttattataa aacaaaagaa gaccaaaaaa atgaggataa ctttggagct
    18841 caaggggctt tcatgcaagg tagtgatacc aaccgtaagg aaccaattat tcaagaatca
    18901 tggttaaaaa agattgctaa ggaagtagat ggtgttagtg gctactatgt aaccaatcaa
    18961 acaaatgctc cagtagctta ccttgaaaaa aaggcaaaga cggttaatat aacaggaatt
    19021 aaccgcactt accttggtat caaaaagttc aaaattaaaa gtggtcgtca attccaagag
    19081 gaggattata accaattctc gagagttatt ttactagaag aaaaattagc tcagagatta
    19141 tttcagacaa atgaagcagc gttaaataaa gtggtgactg taaaaaataa atcttactta
    19201 gtagtggggg tttattcgga tccagaagct ggctcaggtt tatatggaag taattctgat
    19261 gggaatgcta ttttaaccaa tacacagtta gcttcggagt ttggtgctaa agaagcagaa
    19321 aacatatatt ttcatcttaa tgatgtgtcg caatccaatc gcattggtaa agaaataggc
    19381 aaacgtttaa cagacattag ccatgctaag gatgggtact atgataattt tgatatgaca
    19441 tctatagtta aatctatcaa tacccaagtt ggtattatga caggtgttat tggagcgatt
    19501 gcagcaattt ctctcctagt tggtggtatc ggagtgatga atattatgtt agtctctgta
    19561 actgagcgca cgagagaaat tggccttcgt aaagcccttg gggcaactcg acgtaaaatt
    19621 ttagctcaat ttttaattga atcaatggtc ttaacgattt tgggtgggtt aattgggtta
    19681 ttactagcct atggaggtac gatgttaatt gctaatgcgc aagataagat tactccttct
    19741 gtgtcactta acgtggctat aggcagttta attttctcag catttattgg aattatattt
    19801 ggtttattac ctgctaataa agctagcaaa ttaaacccaa ttgatgcttt acgatatgaa
    19861 taaaaaaaca tgtactctgt caagattttt tcttgacaga gtttgtattt atgttatact
    19921 tagtaaggta aagttgttag ttaagctatc aacgaccaaa tataaagaaa aagaaaagag
    19981 actatttaaa aatggcagta aaaatccgtt taactcgtat gggttctaag aagaaacctt
    20041 tctaccgtat caatgttgca gattcacgtg caccacgtga cggacgtttt attgaaactg
    20101 ttggaacata caacccatta gttgcagaaa accaagttac aatcaaagaa gaacgtgttc
    20161 ttgaatggtt atcaaaaggt gcacaacctt cagatacagt tcgtaacctt ctttcaaaag
    20221 ctggagttat gactaaattc cacgatcaaa aattctcaaa ataataaaag cctatggata
    20281 ccattgaaaa tctcattatt gctattgtga aacctttgat ttcacaacca gatcagttaa
    20341 ccataaaaat tcaggatggc ccagaatttt tagagtacca tcttgattta gatacccaag
    20401 acatcggtcg tgttatcggc aaaaagggtc gtacaattac agcgataagg tcgattgtct
    20461 attcggtacc aacacaaggg aaaaaagtaa gactcatcat tgatgagaaa gaataattta
    20521 ttctaaaaga taggaacttc ctgtcttttt tgttatatct acacaaaaat tgtgagtttt
    20581 ttagtctttt agaatataat ggatgtgaaa gggaattttc tgatttttta ttaaggagga
    20641 taagatattg ctatttgatt ttctcgaaga aaaaataaaa ctgaaagcat acctgatgac
    20701 cgccttactt gatgatggag aaattaacct tgatcacttt aaaacagaat atgctttgag
    20761 tgaaaagatt ttacaaggtc ttatttcgga actacaaata ctttattctg atttctacct
    20821 tgttgtcaaa agtagaacaa tggtattgtt ccattacgaa acactttcta agctagagac
    20881 tttgcatacc atttatcgag agtctaatac gttacagttt ttggcatatt ttattgctcc
    20941 tcaaaatgtc agtttttctg aattcacggc taagaaattc atatccgtcg caagtgccta
    21001 ccgaataaag aaaaactgta aactttattt ggagagtgtt aagttatcaa tacaaaaaaa
    21061 taaagtgaca ggtccagaat atcagattag gctgctgatt gctttgttac agtatcattt
    21121 tggttttatt atttatcctt ttgatgctac aagtgaggta atgataaggg attttattga
    21181 atactcgaat tttaaagggt gtgactacga ccttaaagac ttatctgaag aatattacta
    21241 tttttatatt ttggtgaatt tagtttggaa aagaaaagaa tataaagtag aaattcctca
    21301 aacagaatcc tttaatcaac taaaaaaatc tattctctac atggagttat ccaaagcagc
    21361 tcaaaagagt attgctatac atcttggtat agagttgaat gaaggtttac ttgattatct
    21421 atatttagtg ttttgtatta gtgatggtcc tctatttagc aatcgagatg atatcaatat
    21481 caacgaagaa tttatttttg cttcttataa gatatcaaag tctaaagcct ttaagaattt
    21541 ttgtggtcga ttattgggta gagaatttat tgactcgcag cattttaaag atattaatgc
    21601 ctattttttg agacatttta tttgttattg ttattatttt attcctgatt tttatttttt
    21661 aaatacatca aggctgagct attctaagga tttatatcac ctattagata aaggtctggc
    21721 tgatattttt aaccttaaag gagggaattt aactttttca aaacatgaga cagtgctact
    21781 aacaatgcaa ttatctaact taatagagac tttcttagct cctctttctg tatatgtaat
    21841 ttcatctagt aatattagat tacaaaccta tcaagtaatg ttaaaccaat attttacatc
    21901 taagatagcg gaattctttt ttgtaaacta tcaaacgact cagatagatg aaaaattact
    21961 aaaaaaagca gatatcatta ttgctgagag acgctatata tcttctttaa aaaatgactc
    22021 aggtgtggct atacaagata tattagaaat tgggatgaat aataagcagt accatgaact
    22081 acttttacaa gctattatcg aaaaaaggga ccaaaaagtt tttgaatata tttcaaaaat
    22141 gaaacataaa atactttgat agactctata tgagctttat taaaagggat aaaatcgaaa
    22201 tttaaaattt aattcgaaaa gatattgaca gtaatctaga atttcgatat actagatttt
    22261 gtcttttggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    22321 cgaatccagc taccccagtt tcacatggtc cgttggtcaa ggggttaaga caccgccttt
    22381 tcacggcggt aacacgggtt cgaatcccgt acggactatc gtggccagtt gcattttagc
    22441 ggctggtttt tcttttattt tcaaaaatat cggagttact atggaatact ttaacgttgg
    22501 aaaaattgtc aatacacaag gattacaagg cgaaatgcgt gtattaagcg tgactgactt
    22561 tgtagaagaa cgctttaaaa aaggtcaagt gttagcttta ttcgatgaaa agaatcagtt
    22621 tgttatggat attgaaattg ctagtcatcg taagcagaag aattttgaca ttataaaatt
    22681 caaaggaatg tatcatatta atgatattga aaaatataaa ggtttcacac tgaaggtggc
    22741 agaggatcaa ctgagcgacc ttaaagatgg tgaattttat tatcatgaaa ttattggctt
    22801 agatgtctat gaaggggaag agctgattgg aaaaatcaaa gaaattttac aaccaggagc
    22861 aaatgacgtt tgggttgtgg aacgtcatgg aaaacgtgat cttttattac cctatatccc
    22921 gccagtagta ttagaagtgg acctttctaa tcagcgtgtt caagttgaat tgatggaagg
    22981 tttagatgat gaagattgat attttaactt tatttcctga aatgtttgcc cctctggaac
    23041 attctatagt tggtaaggca aaagaaagag gacttcttga aattaattat cacaatttca
    23101 gagaaaatgc tgagaagtct cgacatgttg atgatgagcc ttacggtggg gggcaaggga
    23161 tgcttttacg tgctcaacct atttttgata caattgataa aatagatgct caaaaagcac
    23221 gtgttatttt gctagaccca gcgggcagaa catttgatca ggattttgca gaagaactat
    23281 ccaaagaaga tgaattaatt tttatttgtg gtcactatga aggttatgat gaacgtataa
    23341 aatcattagt aacagacgaa gtatctttgg gagattttgt tcttacggga ggagaattgg
    23401 cggctatgac aatggtcgat gctaccgttc gtctgattcc agaagttata ggtaaagaaa
    23461 caagtcatca ggatgattca ttttcatcgg gcctattaga atatcctcag tatacgcgcc
    23521 catatgatta tttaggcatg acagttcctg atgttctcat gagtggtcat catgagaaca
    23581 tccgaaaatg gcgtttagaa caaagtttgc gaaaaacact agaacgtcga ccagacttgt
    23641 tagaaaatta tgcaatgacg gatgaagaga gacttatctt agagaaaatt aaaacagaaa
    23701 tagaaagaac agatacagta aatgaacaaa acaatttatg atatcactat tgtaggaggc
    23761 ggaccagtag gtctctttgc tgctttttat gctggactac gcggagtatc cgttaaaatt
    23821 attgaaagcc tttcggaatt aggaggccag ccagctatcc tttatcctga gaaaaaaatc
    23881 tatgatattc ctggttatcc ggtgattact ggacgtgaat taattgataa acatattgaa
    23941 caattagaga gatttaaaga ctctattgaa atctgtttaa aagaagaagt tttatcattt
    24001 gaaaaagtag atgatgtttt cacaattcaa acagataaag atcaacattt gtcacgtgct
    24061 attgtttttg cttgtggcaa cggtgctttt gctccgcgtt tactaggttt ggaaaatgaa
    24121 gaaaattatg cagataataa tttattctat aacgttacaa agttagaaca gtttgcaggt
    24181 aaacatgttg ttatttgtgg tggaggcgac tcagctgttg attgggccaa tgaattagat
    24241 aagattgcag ctagtgtagc gattgttcat cgtagagatg cttttagagc gcatgagcat
    24301 agtgtggata tactaaaggc atcaggtgtt agaattttaa ccccttatgt cccaattggt
    24361 ttaaatggag atagtcagag agttagcagc ttggtcgttc aaaaagttaa aggtgatgaa
    24421 gtaattgaat tacctttaga caacttgatt gttagttttg gcttttctac ttctaataaa
    24481 aatttacgtt attggaattt ggattataaa cggtctagta tcaatgtttc ttcacttttt
    24541 gagacgactc aagaaggcgt gtatgctatt ggagatgctg ctaattatcc aggaaaggta
    24601 gaattgattg caacaggata tggggaagca ccagtcgcta taaaccaagc tatcaattat
    24661 atttatcctg atcgagataa ccgtgtagtc cattcaacat cattgataaa ataactataa
    24721 ataaaactga taagtttata aaaaaggatt atatatattg ttaaaaaaat aacaaaactt
    24781 cctatttatt ttgtgatata attaactttg tcgttatgaa tgatattaaa taaatgaagc
    24841 gaggtatttt atgacaaata cggtaacacc aaaagagacg gctggctctt ttatcaataa
    24901 agtgttaggt gggacagcaa ctgcaatcgt tgtagcactc atcccaaatg ctatcctagc
    24961 cacttttttg aaaccatttt tatcttacgg acttgctgca gagttcttac acatcgtgca
    25021 ggtattccaa tttttcacac caattatggc aggatttttg attggtcaac agtttaaatt
    25081 caccccaatg caacagcttg ctgttggagg tgctgcatat attggttctg gtgcgtgggc
    25141 ttacacagaa gttattcaaa aaggagttgc tactggtagt ttccaattgc gtgggattgg
    25201 tgatctcatc aatatgatgc taacagcagc tttagcagtt ttagcagtta agtggtttgg
    25261 taataaattt ggttccctaa ccattatctt gttacctatt atcataggta caggcgtagg
    25321 ctatttagga tggaaattac tcccttacgt ttcatatgtg acaacactta ttggtcaagg
    25381 aattaattct tttactactt tacaaccaat tgcgatgtca attctaattg cgatggcttt
    25441 ttcaatgctt attgtaagtc ctatttcaac agtagcgatt ggtctagcga ttggtcttaa
    25501 tggtatgtca gcaagtgcag cttcaatggg agttgcttca acaacagcag tgctagtttg
    25561 ggcaacgatg aaggctaata agtctggagt tccaattgct attgctttag gtgcaatgaa
    25621 gatgatgatg ccaaatttcc taaagcatcc agtaatggcg attccaatgc ttatgactgc
    25681 tacagtaagt tctctaacag tcccattgtt taaattggtg gggacaccag cttcgtctgg
    25741 ttttgggctt gttggtgcag taggtccgat tgcttcattt gaagcaggag caagtatgtt
    25801 gattgttatc ttatcttggc tcgttattcc ttttgcagta ggttttgtat cacacaaaat
    25861 ttgtaaagac attctgaaac tttataaaga cgatattttc gtttttgaag gtcaaaatta
    25921 atttggaggt tgtatgttag tttatattgc tggttcaggc gctatgggat gtcgttttgg
    25981 atatcaaatt tctaaaacaa atcatgatgt tattttactt gataattggg cagaccatat
    26041 tatggctatt aaagaaaatg gcttgaaggt aacaggtgat acagaggacc ttgttaaact
    26101 tcctatcatg aagccaactg atgcgacaga agaagcagat ttaattattt tgtttactaa
    26161 agcaatgcag ttgccaaata tgcttcaaga tattaaaaaa attattggta agaaaacaaa
    26221 ggtcttatgt cttttaaatg gattaggaca tgaagatgtc attcgtcaat atatccctga
    26281 acacaatatt ttgatgggag taacagtttg gactgcaggg ttgaaaggac cagggcatgc
    26341 tcatctggaa ggagttggtt ctgtaaatct tcaaagtatc gatcctaaca atcaagaagc
    26401 aggtcatcgc gttactgaat tgcttaatga agcaaaatta caggctactt atgacgaaaa
    26461 tgttttgcca aatatttgga gaaaagcttg tgttaacgga acgatgaatt caacttgcgc
    26521 cttgctagat tgtacaattg gtcaactctt tgctagtgaa gacggcgtaa atatggtaca
    26581 tgaaattatc catgaatttg ttactgttgg aaaagcagaa ggtgtagagc ttgatgaaga
    26641 agaaataaca aagtacgtta tggatacatc tgttaaagca gcccatcatt acccttcaat
    26701 gcatcaagac ttagttcaaa atcagcgctt aactgaaatt gatttcttaa atggcgcagt
    26761 gaataaaaaa ggtgaaaacc taggcattga tacaccttac tgtcgattaa tcacacaatt
    26821 aattcacact aaagaaaatg tacttagcat aaaataataa tcaaccggga gaatagtgcc
    26881 cggttttttg atatgcttaa tgataatgat tttgttatac taagaataat aagagttggg
    26941 tatttttttc gagaagtatt tacagtataa aatattacat ttgtattaca aatatttcta
    27001 aaatatgttg acatgttaca aaaaggatat tataatagta gtgtaaataa ataaggaagt
    27061 aggtgtttat gatgaaaaaa ggacaagtaa atgatactaa gcaatcttac tctctacgta
    27121 aatataaatt tggtttagca tcagtaattt tagggtcatt cataatggtc acaagtcctg
    27181 tttttgcgga tcaaactaca tcggttcaag ttaataatca gacaggcact agtgtggatg
    27241 ctaataattc ttccaatgag acaagtgcgt caagtgtgat tacttccaat aatgatagtg
    27301 ttcaagcgtc tgataaagtt gtaaatagtc aaaatacggc aacaaaggac attactactc
    27361 ctttagtaga gacaaagcca atggtggaaa aaacattacc tgaacaaggg aattatgttt
    27421 atagcaaaga aaccgaggtg aaaaatacac cttcaaaatc agccccagta gctttctatg
    27481 caaagaaagg tgataaagtt ttctatgacc aagtatttaa taaagataat gtgaaatgga
    27541 tttcatataa gtcttttggt ggcgtacgtc gatacgcagc tattgagtca ctagatccat
    27601 caggaggttc agagactaaa gcacctactc ctgtaacaaa ttcaggaagc aataatcaag
    27661 agaaaatagc aacgcaagga aattatacat tttcacataa agtagaagta aaaaatgaag
    27721 ctaaggtagc gagtccaact caatttacat tggacaaagg agacagaatt ttttacgacc
    27781 aaatactaac tattgaagga aatcagtggt tatcttataa atcattcaat ggtgttcgtc
    27841 gttttgtttt gctaggtaaa gcatcttcag tagaaaaaac tgaagataaa gaaaaagtgt
    27901 ctcctcaacc acaagcccgt attactaaaa ctggtagact gactatttct aacgaaacaa
    27961 ctacaggttt tgatatttta attacgaata ttaaagatga taacggtatc gctgctgtta
    28021 aggtaccggt ttggactgaa caaggagggc aagatgatat taaatggtat acagctgtaa
    28081 ctactgggga tggcaactac aaagtagctg tatcatttgc tgaccataag aatgagaagg
    28141 gtctttataa tattcattta tactaccaag aagctagtgg gacacttgta ggtgtaacag
    28201 gaactaaagt gacagtagct ggaactaatt cttctcaaga acctattgaa aatggtttac
    28261 caaagactgg tgtttataat attatcggaa gtactgaagt aaaaaatgaa gctaaaatat
    28321 caagtcagac ccaatttact ttagaaaaag gtgacaaaat aaattatgat caagtattga
    28381 cagcagatgg ttaccagtgg atttcttaca aatcttatag tggtgttcgt cgctatattc
    28441 ctgtgaaaaa gctaactaca agtagtgaaa aagcgaaaga tgaggcgact aaaccgacta
    28501 gttatcccaa cttacctaaa acaggtacct atacatttac taaaactgta gatgtgaaaa
    28561 gtcaacctaa agtatcaagt ccagtggaat ttaattttca aaagggtgaa aaaatacatt
    28621 atgatcaagt gttagtagta gatggtcatc agtggatttc atacaagagt tattccggta
    28681 ttcgtcgcta tattgaaatt taaagctaaa tctttttaga tttagcttta ctaatattat
    28741 gtgtgttgga aaaaacagtg gagatttacg tcaaaattat attatttgga tagtatcgaa
    28801 tagatagaat gtggagaaaa gtaggaaata gttaagaaag gtatttatga cgcttcgaga
    28861 attaacaata gaagaattta aagaacattc aggaaattat gattcacaat catttttaca
    28921 aacacctgag atggctaaac ttttagaaaa acgcggctat gatgttaggt atttgggata
    28981 tcaagtagaa aataaactag agataatcag tttatcttat attatgccag tcactggtgg
    29041 ttttcaaatg aaaattgatt caggaccagt tcattcaaat tctaagtatc taaaacaatt
    29101 ttataaagca ttgcaaggct atgccaaatc caacggtgtt ctagaattaa tagttgagcc
    29161 ttttgatgat taccaattat tcactagttc gggagttcct agtaatcagg gaaatgataa
    29221 tctgattgaa gattttacca gttcaggtta tcaccatgat ggtttaacaa ctggttttac
    29281 tggtaaatat ttatcttggc actatgttaa aaatttagaa ggtgtcactt ctgaaacgtt
    29341 actatcttca ttctctaaga caggacgagc tttggttaag aaagcaatgt cttttggaat
    29401 caaggttcgc gttcttaaac gtgatgagct acatttattt aaagagataa caacttctac
    29461 gtcaaataga cgtgattata tggataagtc cttagattat tatcaagatt tttacgatag
    29521 ctttgaaggc aaggctgaat ttgtgattgc cactttaaat tttagagaat acgaccataa
    29581 cttgcaaata aaagctgaag cattggaaaa taagcttaaa ttgcttgatg aacgttttag
    29641 ggaaaatgca gactctccta agtatcatag gcaacgctct gaaataatta atcaactggc
    29701 tagttttgaa acacgacgac aagaagttca gtcatttatt caaaagtatg ataaccaaga
    29761 cgttgtttta gctggtagtc tatttgttta tagtcttaag gagacagttt atttcttttc
    29821 aggatcctac acagaattta ataaatttta tgcaccagca gttcttcagg aatatgttat
    29881 gcaagaggct ctcaaaagag gttctacttt ctataacctc ctaggtattt agggaacttt
    29941 tgatggttca gatagtattc ttcgctttaa acagaatttt aatggttata tcattcgtaa
    30001 aatgggaact tttaactatt atccaagtcc ttttaaatac aaaggaattc agcttcttaa
    30061 aaaggttttg aagagaaatt aaagtatttg taaaagctaa tctttagatt aggctttttt
    30121 agtttattga tagtttttgg aagaaaatgc ttgacattga aagaaaaaga gatataatta
    30181 aattgcaaac gatttcaatt caaggaggga ctatgctaaa gtcaaagaga aaagaaatta
    30241 tcttatcacg acttgagcaa aataagagtg ttactttaga tgaattaacg tctatcttag
    30301 agacttctga atctacagtt cgcagggatt tagatgaact tgaatctgaa ggttttttaa
    30361 aaagggtgca tggtggtgct gaattaccat actcacttgg ccaggaatta agtaatcaag
    30421 aaaaatctat caaaaacgtt caaaaaaaat tggatattgc tagacaaact gcaaagctga
    30481 tagcaaagca agatgttata tttattgatg ccggaactac gacagaatta ttaattgatt
    30541 ttctccctca cgagcaatta acggtggtga cgaattctat tcatcatgct gcaaagttag
    30601 ttgatagagg gataaaaaca atcattatcg gaggagcggt taaacattca acagatgcta
    30661 gtattggcca ggtagccatt aatcagatta ggcagataac ggttgacaaa gcttttctgg
    30721 ggatgaatgg tatagatgaa gtttatttaa ctacaccaga tttagaagaa gctgctatta
    30781 aagaagctat tattaataat agtcaacaaa cctttattct aatggattca tctaaaattg
    30841 gacaagttac atttgctaaa gtgaaagaga taaatgacat taatttagta actaataaga
    30901 ctgattcaga acttatgaca ataataaaag agaaaatgaa ggtgattcaa gtatgattta
    30961 tacagtaaca ttaaatccat cgattgattt tattgttcgt ctagatacac tattgttggg
    31021 tagtgttaac cgtatgacta gcgatgataa atatgtaggt ggtaaaggaa ttaatgttag
    31081 tcgcatatta aaacgcttaa aaattgacaa tactgctaca ggttttatcg gtggatttac
    31141 aggacatttt gttgaagatg gacttgtatc agaaggtatc aaaactgact ttgtttctgt
    31201 taatgaagat acacgtatca acgttaaagt aaaagcaaaa attgaaactg aaatcaatgg
    31261 cgggggtcct aggataacaa atgaacaact tcatcgtcta gagaaacttc tttcgagact
    31321 aacacctgag gacacagtag ttttcgcagg ttcagcacca gctagccttg gaaataaggt
    31381 ctataataca ttgattccaa ttgctaaaaa gacaggagcg gaagttgttt gcgattttga
    31441 aggtcagaca cttttagacg ctcttgctta tcagcctctt ttagtaaaac cgaataacca
    31501 tgaattagca gatatttttg gcgtagagct agaaggtctg cctgatatcg aaaaatatgc
    31561 acataagatt ctagataaag gagctaagaa tgttatcgtt tctatggcag gggatggtgc
    31621 attgttagta actccagaag cttcatattt tgcaaaaccg attaagggtg aagtgaaaaa
    31681 ttccgttggg gctggtgatt caatggtagc tggctttaca ggagaatttg ttaagtcaaa
    31741 aaatccagta gaagcactaa aatggggcgt agcttgtggt acagcaacca ctttctcaga
    31801 tgatttagca acagctgagt ttatccaaga tatttataat aaagttgagg tagaaaaatt
    31861 atgaaaatcc aagacctact aaaaaaagaa gttatgatta tggatttaaa agcaaccagt
    31921 aaagaggctg caattgatga aatgattaca aaattagttg acacaggtgt tgtgacaaac
    31981 tttgctattt ttaaagatgg tatcatgaag cgtgaggcac aaacatcaac tggattaggt
    32041 gatggtatcg ctatgccaca tagtaaaaat gctgccgtta aagaagctac tgtccttttt
    32101 gcaaaatctg catcaggtgt tgattacgaa gcacttgatg gacagccaac tgatttattc
    32161 tttatgattg ctgcgccaga tggtgctaat gatacgcatt tagctgcctt ggcagaacta
    32221 tctaaatatc ttttaaaaga agggttcgct gatcaattac gtcaagcaaa aacgccagat
    32281 gacattattg ctacttttga tagtaatagc atttctcaag aaacagtagc tccacaaaca
    32341 gtacaatcaa caagtaaagg ttcagattat attgttgcag taacagcttg cactacggga
    32401 attgcccaca cgtatatggc agaagaagct ttaaagaaaa aagcagctga aatgggtgta
    32461 ggtattaaag ttgaaacaaa tggcgcttct ggtgtaggaa ataagttgac ttcaagtgat
    32521 attgcaaggg ctaagggtgt tattattgca gcagataagg ctgtagagat ggatcgtttt
    32581 gatggcaaac ctttggtatc acgtccggta gctgatggta ttaaaaaaag tgaagactta
    32641 attaacatta ttctcgataa taaggcacaa acctatcatg caaaaaatca aaatgataaa
    32701 caatcaggtg aatctgatgg aaaatcaggc ttaggctctg ctttttataa acaccttatg
    32761 ggtggtgtat cacaaatgtt accattcgtt attggtggtg gtattatgat tgcgattgct
    32821 ttcctttttg ataacatact tggtgtacca aaagaccaat taagtaattt aggttcatac
    32881 catgaaattg cagccttatt caaaaatatc ggtggcgcag catttgcatt tatgttacca
    32941 gtattggcag gttatatcgc ttattcaata gctgaaaaac caggactagt agctggcttc
    33001 gtagctggtt caattgcatc aagtggactg gcattcggta aagtaccttt tgcagaaggt
    33061 gggaaagcaa ccttagcatt agcaggtgtg ccatcaggtt tcttaggggc tttagtaggt
    33121 ggtttcttag ctggtggagt tatcctacta cttagaaaat tactttcagg tctcccaaaa
    33181 tcgcttgaag gtattaaatc aattcttcta tacccattgt taggtgtttt gattacaggt
    33241 ttcttgatgt tgctcgtaaa cattccaatg gcagctatca acacagctct taacactttc
    33301 ttacaaggtt tatctggtag ttcagcagtc ttgatgggct tactagttgg tggtatgatg
    33361 gctgtagata tgggtgggcc tgttaataaa gcagcttatg tttttggtac aggtactctt
    33421 gcagcaactg ttgcaaatgg cggctctgtt gttatggcag ctgtaatggc tggaggaatg
    33481 gttccaccgt tagcagtatt tgtggcaact ttattattta aagataaatt taataatgaa
    33541 gaacgtcaat ctggtctaac aaatatcgtt atggggttat cttttatcac tgaaggtgct
    33601 attccgtttg gtgctgctga cccagctcgt gcaatcccta gctttattgt aggttcagcc
    33661 ttaactggag cacttgttgg tttagctggt atcaagttga tggctcctca tggagggatt
    33721 tttgtcatcg cattaacaag taatccttta ctttatattc tatttattct aattggagcg
    33781 atagtctcag gtgtactctt tggcttgttc cgtaaaacaa aatagttaat ctcaacttat
    33841 ccaaatttac attgaataaa aggtgaataa actatcgtta ttgggtctat tcgccttttt
    33901 atgttgtaaa agaatggtag attaatcatt aataattgct aatctttttt caataggata
    33961 tgatttattt aagcaaaaaa atggataata tgattgagga aaggaggtaa gcaatgatta
    34021 aatggaatga aaaagaaaca aaactagttg atacaagttt ttcaattatg ttgtatctaa
    34081 aaacgctatt tgcagtcgga caagtagtag ctggcgtttt attatattat tttaaaccta
    34141 cacaaataaa acatctccta tttcttttaa ctgaaggtga agttcacggt gggattcatg
    34201 gttttatttc tgctcatttc ttgaaaataa gcagttatat taatgattcg gctagtcatt
    34261 tagccgctat ctatctatta attc
//
LOCUS       DATRUK010000016        33792 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.016, whole genome shotgun sequence.
ACCESSION   DATRUK010000016 DATRUK010000000
VERSION     DATRUK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33792
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.016"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(22..1266)
                     /locus_tag="VBE34_000720"
     CDS             complement(22..1266)
                     /locus_tag="VBE34_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEO7263456.1"
                     /translation="MGLFGFGKPKDEALEAKISSLESKLKDKQSILDDLTRQIKVAKD
                     ILKLDDELKKRQSELKALQKEVIVLNDDLDLQEYGFFERKYKFSDSTKYKEELETLRK
                     RQKQLVKDKKAGVIVRPMLLDNSKSKGRAMQNQLIKAMIRGFNGEVDAHLVKVTATNL
                     SKKVDAVVKSFNQLNKMYSRNLVCLSNDYLELKISELQLAVEYELQKLEEKELLREQR
                     AKEREDKKLQAELAAKRKQIEKDRKHFKQMLDNVEELLKTANEEEKEKLNLQLAEYQD
                     KLSELDELEEDIDYREGHATAGYVYIISNIGAFGEDVYKIGVTRRLDPMERIRELGSA
                     SVPFQFDVHALIFSEEAFSLEKELHNRFEKNKINKVNGRKEYFKVPFSDIKAVLEEHK
                     ELAIELTEKAEAFEYRQSLMTK"
     gene            complement(1276..2349)
                     /gene="purK"
                     /locus_tag="VBE34_000721"
     CDS             complement(1276..2349)
                     /gene="purK"
                     /locus_tag="VBE34_000721"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEO7263457.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPASDCPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSAGQLPQGTDLLRISQNR
                     IFEKDFLANKAGVTVAPYKVVTSSLDLEGLDLTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAECVLEEFVDFDLEISVIVSGNGKDVTVFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKELQLSGTLCVEMFVTADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGQPLPPIKLHDPAVMLNVLGQHVQQAIDYVAQNPSAHLHMYGKLE
                     AKHNRKMGHVTVFVEDADEVEEF"
     gene            complement(2336..2824)
                     /gene="purE"
                     /locus_tag="VBE34_000722"
     CDS             complement(2336..2824)
                     /gene="purE"
                     /locus_tag="VBE34_000722"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEO7263458.1"
                     /translation="MQPIISIIMGSKSDWTTMQKTAEVLDNFGIAYEKKVVSAHRTPD
                     LMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSNE
                     LI"
     gene            complement(3110..4372)
                     /gene="purD"
                     /locus_tag="VBE34_000723"
     CDS             complement(3110..4372)
                     /gene="purD"
                     /locus_tag="VBE34_000723"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEO7263459.1"
                     /translation="MKLLVVGSGGREHAIAKKLLVSKGVDQVFVAPGNDGMTLDGLGL
                     VNIVISEHSRLIDFAKENEIAWTFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTYSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPTAQDHKCAY
                     DGDMGPNTGGMGAYAPVPHLPQSVVDTAVETIVKPILEGMITEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFAENGKALLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIK"
     gene            4654..5457
                     /locus_tag="VBE34_000724"
     CDS             4654..5457
                     /locus_tag="VBE34_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEO7263460.1"
                     /translation="MTIYDQIESALDLMTDLEHEIACYFMGQPISKDALASSIVTKQL
                     HISQAALTRFAKKCGFKGYREFVFEYLKSHETISQQLYGLQNDNTKKVFMNYQEMVSK
                     SADIIDEEQLLEVSHMIEQADRVYFYGKGSSSLVAKEFKIRLMRLGVICEALDDTDSF
                     SWTNSIVNDRCLVIAFSLSGNTNSVIGALKIASCHGAKTVLFTKQPHTIDYAFDKIIQ
                     VASARHLDYGNRISPQIPMLIMVDIIYAQFLDINKIEKERIFRETIIQR"
     gene            complement(5480..6457)
                     /locus_tag="VBE34_000725"
     CDS             complement(5480..6457)
                     /locus_tag="VBE34_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO7263461.1"
                     /translation="MIETMSLDDMREYLGQDQIPEDFDDFWKKQTMKYQGNIEYRLDK
                     KDFNITFAQAYDLHFKGSNNSIVYAKCLFPKTNKPYPVVFYFHGYQNQSPDWSDQLNY
                     VAAGYGVVSMDVRGQAGQSQDKGHFDGITVKGQIVRGMISGPNHLFYKDIYLDVFQLI
                     DIIATLESVDSNQLYSYGWSQGGALALIAAALNPKIVKTVAVYPFLSDFRRVLDLGNV
                     SEPYDELFRYFKYSDPFHKTENNVLKTLAYIDVKNFAHRISCPVVLLTALKDDICPPS
                     SQFAIFNRLTSTKKHLLLPDYGHDPMTVQVKDYIFDQLTGSQFTKQKIE"
     gene            complement(6465..7346)
                     /locus_tag="VBE34_000726"
     CDS             complement(6465..7346)
                     /locus_tag="VBE34_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO7263462.1"
                     /translation="MTRTVAIDIGGTMIKHGIVDNLGCIVEASELATEAYKGGPGILQ
                     KVCQIIDNYLTEGSIDGIAISSAGMVDPDEGCIFYSGPQIPNYAGTQFKKVLEDTYQV
                     RTEIENDVNCAGLAEAVSGSAKDSSIALCLTIGTGIGGCLIIDKTVFHGFSNSACEVG
                     YMHLSDGDFQDLASTTALIADVAKAHGDEINRWDGRRIFQEAKEGNEKCIASIDRMIN
                     YLGQGIANMVYVVNPEKVVLGGGIMAQKDYLQDKLSESLKRNLVTSLAEKTIIVFAQH
                     ENQAGMLGAYYHFKNRG"
     gene            complement(7363..8280)
                     /locus_tag="VBE34_000727"
     CDS             complement(7363..8280)
                     /locus_tag="VBE34_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEO7263463.1"
                     /translation="MKDLQKYQGIIPAFYACYDDKGDICPERVKALTNYFIDKGVQGL
                     YVNGSSGECIYQSVADRKLVLENVMSVAKGKLTVIAHVACNNTKDSVELAMHAEAMGV
                     DAIAAIPPIYFRLPEYAIADYWNTISQAAPQTDFIIYNIPQLAGVALTSDLYRKMLQN
                     PQVIGVKNSSMPVQDIQNFVAIGGENHIVFNGPDEQFLGGRLMGAAAGIGGTYGVMPE
                     LYLTLNQLIVDKDLEKARELQFTINDIITKLCSGHGNMYAVIKAVLEINEQLTIGSVR
                     LPLASVTEEDKPIIKEAAEMIRHAKKQFC"
     gene            complement(8277..8939)
                     /locus_tag="VBE34_000728"
     CDS             complement(8277..8939)
                     /locus_tag="VBE34_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEO7263464.1"
                     /translation="MKKANQLIAAIFDLNNPFMQGCNVVFDLALLNLLFMITCLPLVT
                     IGAAKISLYRTLWQKLEGDQTNLLILYIKHLKKEWFQGMLLGLVELSILVVIIFDLTI
                     LHYQIGFIVPFLKIICYAFLLLTVMTSIYLFPMAARYEMSLLDTVKKSFIMACLNLKW
                     TGVLMFLLIMTWFIMVQSSLLFMLTVSAIFIFAYTAFAYFKIIILQKQFAYFSKQQGD
                     YQ"
     gene            complement(8959..9402)
                     /locus_tag="VBE34_000729"
     CDS             complement(8959..9402)
                     /locus_tag="VBE34_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEO7263465.1"
                     /translation="MIYDHLLNLTHYKDINPNLDLAIDYLLSHDLRNLDIGTYHISPE
                     VILMVQSNQLSESFDHIFEYHKKYLDIHYVIEGYEVIKLGKGDKVEVEEYLGDIGFVK
                     CSEETSFDLRDNYIAFFFPEEAHQPNGMGSLGNYVKKGVLKVLMA"
     gene            complement(9415..10245)
                     /locus_tag="VBE34_000730"
     CDS             complement(9415..10245)
                     /locus_tag="VBE34_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO7263466.1"
                     /translation="MKKKTFSAYNFLTALILCLLTVLFIFPFYWIMTGAFKSQPDTII
                     IPPQWWPKAPTLENFKALTVQNPALRWLWNSVFISIMTMFLVCCTSSMAGYVLAKKRF
                     YGQKILFSLFIAAMALPKQVVLVPLVRIINFMGIHDTLWAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFINVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(10255..11142)
                     /locus_tag="VBE34_000731"
     CDS             complement(10255..11142)
                     /locus_tag="VBE34_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO7263467.1"
                     /translation="MRTNKLKMRETMIAYAFLAPILLFFLIFVFAPMVMGFVTSFFNY
                     SMTQFTFIGLANYNRMFHDSIFMKSLINTVIIVIGSVPVVVFFSLFVAANTYEKNVFS
                     RSFYRCVFFLPVVTGSVAVTVVWKWIYDPMSGILNYILKSGHVIEQNISWLGDKHWAL
                     LAIIIILLTTSVGQPIILYIAAMGNIDNSLCEAARVDGANEMQVFWQIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIALISFAQFKILGNDVEY"
     gene            complement(11230..12546)
                     /locus_tag="VBE34_000732"
     CDS             complement(11230..12546)
                     /locus_tag="VBE34_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7263468.1"
                     /translation="MSIKKSVIGFCLGAAALSMFACVDSSQSVMAAEKDKVEITWWAF
                     PTFTQEKAKDGVGTYEKKVIKAFEKKNPNIKVKLETIDFTSGPEKITTAIEAGTAPDV
                     LFDAPGRIIQYGKNGKLADLNDLFTDQFIKDVNNKNIIQASKSGDKAYMYPISSAPFY
                     MAFNKKMLKDAGVLKLVKEGWTTSDFEKVLKALKNKGYTPGSFFANGQGGDQGPRAFF
                     ANLYSAPITDKEVTKYTTDTKNSVKSMKKIVEWIKKGYLMNGSQYDGSADIQNFANGQ
                     TAFTILWAPAQPKTQAKLLESSKVDYLEVPFPSEDGKPDLEYLVNGFAVFNNKDENKV
                     KASKKFITFIADDKKWGPKDVIRTGAFPVRTSFGDLYKGDKRMMKISKWTQYYSPYYN
                     TIDGFSEMRTLWFPMVQSVSNGDEKPADALKDFTQKANDTIKKAAK"
     gene            complement(12593..13291)
                     /locus_tag="VBE34_000733"
     CDS             complement(12593..13291)
                     /locus_tag="VBE34_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEO7263469.1"
                     /translation="MPHLSKEAFKKQIKNGIIVSCQALPGEPLYTESGGVMPLLALAA
                     QEAGAVGIRANSVRDIKEIQEVTNLPIIGIIKREYPPQEPFITATMTEVDQLATLDIA
                     VIALDCTLRERHDGLSVAEFIQKIKRKYPKQLLMADISTFEEGKNAFEAGVDFVGTTL
                     SGYTDYSRQEEGPDIELLNKLCQAGIDVIAEGKIHTPKQANEINHIGVAGIVVGGAIT
                     RPKEIAERFISGLS"
     gene            complement(13538..14842)
                     /locus_tag="VBE34_000734"
     CDS             complement(13538..14842)
                     /locus_tag="VBE34_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO7263470.1"
                     /translation="MKMNKKVLLTSTMAASLLSVASVQAQETDTTWTARTVSEVKADL
                     VKQDNKSSYTVKYGDTLSVISEAMSIDMNVLAKINNIADINLIYPETTLTVTYDQKSH
                     TATSMKIETPATNAAGQTTATVDLKTNQVFVADQKVSLNTISEGMTPEAATTIVSPMK
                     TYSSAPALKSKEVLAQEQAVSQVAANEQVSPAPVKSITSEVPAAKEEVKPTQTSVSQL
                     TTVSPASVAAETPAPVAKVAPVRTVAAPRVASAKVVTPKVETGASPEHVSAPAVPVTT
                     TSPATDSKLQATEVKSVPVAQKAPTATPVAQPASTTNAVAAHPENAGLQPHVAAYKEK
                     VASTYGVNEFSTYRAGDPGDHGKGLAVDFIVGKNQALGNEVAQYSTQNMAANNISYVI
                     WQQKFYSNTNSIYGPANTWNAMPDRGGVTANHYDHVHVSFNK"
     gene            complement(14989..15888)
                     /locus_tag="VBE34_000735"
     CDS             complement(14989..15888)
                     /locus_tag="VBE34_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEO7263471.1"
                     /translation="MNKWLVKASSLVVLGGMVLSAGSRVLADTYVRPIDNGRITTGFN
                     GYPGHCGVDYAVPTGTIIRAVADGTVKFAGAGANFSWMTDLAGNCVMIQHADGMHSGY
                     AHMSRVVARTGEKVKQGDIIGYVGATGMATGPHLHFEFLPANPNFQNGFHGRINPTSL
                     IANVATFSGKTQASAPSIKPLQSAPVQNQSSKLKVYRVDELQKVNGVWLVKNNTLTPT
                     GFDWNDNGIPASEIDEVDANGNLTADQVLQKGGYFIFNPKTLKTVEKPIQGTAGLTWA
                     KTRFANGSSVWLRVDNSQELLYK"
     gene            complement(16081..17628)
                     /gene="purH"
                     /locus_tag="VBE34_000736"
     CDS             complement(16081..17628)
                     /gene="purH"
                     /locus_tag="VBE34_000736"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEO7263472.1"
                     /translation="MTKRALISVSDKSGIIDFAKELKNLGWDIISTGGTKVALDDAGV
                     ETIAIDDVTGSPEMMDGRVKTLHSNIHGGILARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDVTNDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     SQTTFKTRQRLAAKAFRHTAAYDALIAEYFTAQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAEKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEVTALEFAWKAIKYVKSNGIIITNDHMTLGLGVGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQESIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(17648..18400)
                     /locus_tag="VBE34_000737"
     CDS             complement(17648..18400)
                     /locus_tag="VBE34_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7263473.1"
                     /translation="MKLVKNLEIVESIFGDWDETIIWSCVQGIMGEVFVDSLDQPKSS
                     LAKLGRKSSFGFLAGQPTLFLLEVCSGEDIILVPQHKGWSDLIESTYGQNAHSFKRYA
                     TKKDTLFERSRLEKFVTQLPNGFELRVIDEKVYNSCLEKEWSQDLVANYATYQYYKKQ
                     GIGYVIYYQGNIIAGASSYSTYKNGIEIEVDTHPDFRRRGLATIVAAQLILTCLDKGI
                     YPSWDAHTRTSLNLSEKLGYEFSHEYIAYEID"
     gene            complement(18423..18971)
                     /gene="purN"
                     /locus_tag="VBE34_000738"
     CDS             complement(18423..18971)
                     /gene="purN"
                     /locus_tag="VBE34_000738"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEO7263474.1"
                     /translation="MKIAVFASANGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAVVDLLDKHEIDLVCLAGYMKIVGETLLSAYEGRIINIHP
                     AYLPEFPGAHGIKDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPDVLDSLGIKRK"
     gene            complement(19138..20160)
                     /locus_tag="VBE34_000739"
     CDS             complement(19138..20160)
                     /locus_tag="VBE34_000739"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEO7263475.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVSRTERAGVMGALGGFG
                     GVFDLSKTGVREPILVSGTDGVGTKLMLAIKYQQHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYIATGKNNPVKLEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQLIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVQAALPLIKEELVNGIGHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            complement(20188..21642)
                     /locus_tag="VBE34_000740"
     CDS             complement(20188..21642)
                     /locus_tag="VBE34_000740"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEO7263476.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNPSFMGKVKE
                     ALSTVKGGFAYLLMTEDKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDSGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEETSFYIQKVK"
     gene            complement(21876..25601)
                     /locus_tag="VBE34_000741"
     CDS             complement(21876..25601)
                     /locus_tag="VBE34_000741"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEO7263477.1"
                     /translation="MNKRIFVEKKADFDIKSASLVKELTHNLQLTSLKDLRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDCLLTETEITAELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLLGSDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPSLDFFETYKADDFAAYKAEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQATYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRDGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRPSDVDTFIA
                     ACNKENIDAVVVATITAKPNLVMTWDGETIVDLERRFLDTNGVRVVVDAKVVDKDLTV
                     PEVRTTSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFDQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFETIQAQHKIT
                     AASAVKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADSVKIG
                     QTQAAFTLTVNGNDLAGASLLSVFEGKLEEVYPTEFEQADALEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEAAIADSVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNKKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLVGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQALFASAVKYFTGK"
     gene            complement(25724..26428)
                     /locus_tag="VBE34_000742"
     CDS             complement(25724..26428)
                     /locus_tag="VBE34_000742"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEO7263478.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNMAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVTAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHMKFLGIVNDEEIAYLKGETRRINEL
                     LKDWFAQIGLNLIDFKLEFGFDKDGKIILADEFSPDNCRLWDAEGNHMDKDVFRRDLG
                     SLTDVYQVVLEKLIAL"
     gene            complement(26552..26791)
                     /locus_tag="VBE34_000743"
     CDS             complement(26552..26791)
                     /locus_tag="VBE34_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085641.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO7263479.1"
                     /translation="MSRDEVFEKMLELLRQQLGDPQLDITPESSLHDDLAIDSIALTE
                     FIINLEDVFHLEIPDEAVEHMSSVQQLLDYIIEHK"
     gene            complement(26802..27794)
                     /gene="plsX"
                     /locus_tag="VBE34_000744"
     CDS             complement(26802..27794)
                     /gene="plsX"
                     /locus_tag="VBE34_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEO7263480.1"
                     /translation="MKKIAVDAMGGDYAPKAIVEGVNQAISDFSDIEVQLYGDQKKIE
                     KYLTVTERVSIIHTEEKINSDDEPAKAVRRKKQSSMVLGAKAVKDGVAQAFISAGNTG
                     ALLAAGLFVVGRIKGVDRPGLMSTMPTLDGVGFDMLDLGANAENTASHLHQYAILGSF
                     YAKNVRGIEVPRVGLLNNGTEETKGDSLHKEAYELLAAEPSINFIGNIEARDLMSSVA
                     DIVVTDGFTGNAVLKTMEGTAMNIMGSLKSSIKSGGVKAKLGALLLKDSLYQLKDSMD
                     YSSAGGAVLFGLKAPIVKCHGSSDSKAVYSTLKQVRTMLETQVVDQLVDAFTDE"
     gene            complement(27872..28750)
                     /locus_tag="VBE34_000745"
     CDS             complement(27872..28750)
                     /locus_tag="VBE34_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7263481.1"
                     /translation="MELIFRIFEMKKTLYITNKLFFANWFVPVIGAVLIQVIGIIVSR
                     RLVVKLLPDDYKFNLYIALLLFIPILASILLFLIYFQKRDFTSLGFTKDNNVLTTISG
                     FITGSLLMFFIYICHWLSGFVEISSSNSFSFGPFFFLLLAYFIQSLTEEILFRGYVMT
                     TVTKFKGSFAGVLCNSMLFSFIHFRNYGITAIALFNLFLLGIIFSILFNMTKNILFVT
                     GVHTAWNFTMGCVLGNKVSGGYSPVSLFRITENSSFAIWSGGDFGFEGGVLCSLILVG
                     CLAYFSLERVKVSVRY"
     gene            complement(28813..29574)
                     /gene="recO"
                     /locus_tag="VBE34_000746"
     CDS             complement(28813..29574)
                     /gene="recO"
                     /locus_tag="VBE34_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEO7263482.1"
                     /translation="MRVSQTYGLVLYNRNYREDDKLVKIFTETEGKRMFFVKHASKSK
                     FNAVLQPLTIAHFILKINDNGLSYIDDYKEVLAFQETNSDLFKLSYASYITSLADVAI
                     SDNVADAQLFIFLKKTLELIEDGLDYEILTNIFEVQLLERFGVALNFYDCVFCHRAGL
                     PFDFSHKYSGLLCPNHYYKDERRNHLDPNMLYLINRFQSIQFDDLQTISVKPEMKLKI
                     RQFLDMIYDEYVGIHLKSKKFIDDLSSWGSIMKSD"
     gene            complement(29564..30739)
                     /locus_tag="VBE34_000747"
     CDS             complement(29564..30739)
                     /locus_tag="VBE34_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7263483.1"
                     /translation="MTLEKRFNKYLDRIEVSLIRQFDQSISDIPGMVKLTLGEPDFTT
                     PDHVKEAAKSAIDANQSYYTGMSGLLALRQAAADFAKDKYNLTYNPDSEILVTIGATE
                     ALSASLIAILEAGDVVLLPAPAYPGYEPIVNLVGADIVEIDTRENDFRLTPEMLETAI
                     IQQGEKLKAVLLNYPTNPTGITYSRQEIAALAEVLKKYDIFVISDEVYSELTYTGQQH
                     VSIAEYLPNQTILINGLSKSHAMTGWRVGLVYAPEASTAQIIKSHQYMVTAASTISQF
                     AGVEALSVGKNDTLPMRQGYIKRRDYIIDKMSKLGFKIIKPSGAFYIFAKIPDSYPQD
                     SFKFCQDFAYQQAVAIIPGVAFGKYGEGYIRLSYAASMEVIETAMARLKVFMESYEG"
     gene            complement(30847..31815)
                     /locus_tag="VBE34_000748"
     CDS             complement(30847..31815)
                     /locus_tag="VBE34_000748"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO7263484.1"
                     /translation="MSYSNLKLFALSSNKELAKKVSQTIGIPLGQSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAESVSVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRQ
                     GLVGDDVVVVSPDHGGVTRARKLAQCLKTPIAIIDKRRSVTKMNTSEVMNIIGNIKGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQNSAIEKLIVL
                     DTIYLPEERLIDKIEQISIAELIGEAIIRIHEKRPLSPLFEMNKLK"
     gene            complement(31939..33264)
                     /locus_tag="VBE34_000749"
     CDS             complement(31939..33264)
                     /locus_tag="VBE34_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7263485.1"
                     /translation="MKKRILSAVLVSGVTLGTAAVTVNADDFDSKIAATDSVINTLSG
                     QQAAAQNQVTAIKGQVGALESQQSELEAQNAQLEAVSQQLGQEIQTLSNKIVARNESL
                     KKQVRSAQKGNLTNYINTILNSKSVSDAVNRVVAIREVVSANEKMLAQQEADKAALEA
                     KQIENQNAINTVAANKQAIENNKAALATQRAQLEAAQLELSAQLTTVQNEKASLIQAK
                     AQAEEAARKAAEAQAVAEAKAQAESVAKAQAAAQVESATAPTETVQTQPRTEIKPSNL
                     TATSSATTVAKTTATATNEPKVTQPSVVTKAVEAPKAVVSSTPRAVSKPVVRSYDSSN
                     TYPMGQCTWGAKSMASWVGNYWGNANQWGASARAAGYSVGTTPRVGAVAVWPYDGGGY
                     GHVAVVTSVANNSSIQVMESNYAGNMSIGNYRGSFNPSASGSVYYIYPN"
     gene            complement(33500..33587)
                     /locus_tag="VBE34_000750"
     tRNA            complement(33500..33587)
                     /locus_tag="VBE34_000750"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33551..33553),aa:Ser,seq:gct)
     gene            complement(33601..33674)
                     /locus_tag="VBE34_000751"
     tRNA            complement(33601..33674)
                     /locus_tag="VBE34_000751"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33638..33640),aa:Ile,seq:gat)
     gene            complement(33709..33779)
                     /locus_tag="VBE34_000752"
     tRNA            complement(33709..33779)
                     /locus_tag="VBE34_000752"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33745..33747),aa:Gly,seq:tcc)
BASE COUNT        10292 a         7244 c         5822 g        10434 t
ORIGIN      
        1 aatacagtct tttctatata attattttgt catcagactt tgtctatatt caaatgcttc
       61 tgctttttca gtcaactcga tagcaagttc tttatgctcc tctaacactg ctttgatgtc
      121 agaaaatggg actttaaaat actcttttcg cccattaact ttattaattt tatttttttc
      181 aaatctgtta tgtaactctt tttctaatga aaaagcttcc tcactaaaaa ttaaggcatg
      241 tacatcaaat tgaaatggaa cagatgcact ccctaattcg cgaatacgtt ccataggatc
      301 tagacgcctt gtaacaccaa tcttgtaaac atcttctcca aatgcaccaa tatttgaaat
      361 gatataaaca tatccagcag ttgcatgccc ttctcgataa tcaatatctt cttctaactc
      421 atctagttct gataatttat cttggtattc tgctaattga agatttaatt tttccttttc
      481 ttcctcatta gctgttttaa gtaattcttc aacgttatct aacatctgtt taaagtgttt
      541 tctatctttt tcaatttgtt ttctcttagc agctaattct gcttgaagtt tcttatcttc
      601 tctttccttc gcacgttgtt cacgaagaag ttctttttct tctaatttct gaagttcata
      661 ttctactgct aattgtaact ctgaaatttt tagctcaaga tagtcattag atagacatac
      721 caaatttctt gaatacatct tattaagctg attaaatgat ttaacaactg catctacttt
      781 tttacttaga ttagtagccg ttacctttac caggtgagca tcaacttctc cattaaaacc
      841 tcttatcatc gctttaataa gttgattttg cattgctcgt ccttttgatt tactgttatc
      901 cagcaacata ggtcttacaa taacacccgc ttttttatct tttactaact gtttttgcct
      961 ctttctcaga gtttccaatt cttctttata cttagtacta tctgagaact tatattttct
     1021 ctcaaaaaat ccgtactctt gaagatctaa atcatcatta agaactatta cctctttttg
     1081 taaagcttta agttctgatt ggcgtttctt aagttcatca tcaagtttta aaatatcttt
     1141 agcaaccttt atctgccttg ttaaatcatc caaaatagat tgtttatctt ttaacttact
     1201 ttctaaacta ctgattttag cttccaaagc ctcatcttta ggctttccga atccaaatag
     1261 acccatttat tccccctaaa actcctccac ctcatccgca tcttccacaa acaccgttac
     1321 atgtcccatt ttgcggttat gtttcgcttc tagtttacca tacatgtgga ggtgagcgct
     1381 agggttttgg gcaacatagt caatcgcctg ctggacatgt tgtccaagga cattaagcat
     1441 aacggctggg tcatggagct tgattggtgg gagaggttgc cccagtacac ccaagatgtg
     1501 ggtgtcaaac tgtgaaaagt cacaggcttc gatagaatag tgccctgagt tgtgggggcg
     1561 tggggcgatt tcattgacga tgatatcatc tgcggtcaca aacatttcca cacagagggt
     1621 tcctgatagc tggagttcct tggcaatctg taccgccatt ttcttagcct tgtcagctag
     1681 ttggtctgag atgcgagctg gtacgatagt ttttgatagg atattgttgc ggtggatatt
     1741 ttcctgaacg ggaaagaccg tcacatcctt gccatttcca gacacgatga ctgagatttc
     1801 aaggtcaaag tcgacaaact cttccaagac acactcagct gagttggcta attgttgcgc
     1861 ctctggcagg tcttctgctg agcggataac cttttgccca tgaccgtcat aaccacctgt
     1921 cgctgtcttg aggacatagg tcttggtcaa gtcaagcccc tctaggtcaa ggctagacgt
     1981 caccacctta tagggagcga cagtcactcc agccttattt gccagaaagt ctttttcaaa
     2041 gatacggttt tgagaaatgc ggagcagatc agtcccctgc ggtagctgac cagctgacac
     2101 aacggcatcc agaccatcgg catcaacatt ttcaaactca taggtcaaaa catcgcaacg
     2161 cgcagctaat tgtcccaaag cctcaacgtc atcgtaaggc gccacaatca cctcgctaac
     2221 gcgggaggca gggcagtcgc tagcaggatc cagcgtaatg accttgtggc ccatgtagat
     2281 agccgcaatc gccatcatct gccccagctg accaccaccg ataatcccaa tggtcttaaa
     2341 tgagttcatt gcttgactcc tctgcgattt ttccttgttc ctcatggaaa tgagctagcg
     2401 catcagccag attttggtcc tcgatagaga gaatacggag ggctgttagg gcagcgttag
     2461 tcgctcccgc ttctccgata gccatggttg ccacaggcac accgccaggc atctgcacaa
     2521 tggaatagag cgaatccaag cctgaaaggg cacgtgattt gacaggcaca ccgataacag
     2581 gcagggttgt tttagctgct accataccag gcaagtgggc tgccccacct gctccagcaa
     2641 tgatgatttt gataccacga ccacgcgcct cctcagcatg cttgaacatg aggtctggcg
     2701 tacggtgggc agagacgact ttcttctcgt aagcaatgcc aaagttatct agaacttcag
     2761 ccgttttttg catggttgtc cagtcggatt tggaacccat gataatagaa ataattggtt
     2821 gcataatatt aagatacaaa gggacgtttc gacttgtcga aaattttcgt agaaaaatat
     2881 gcggtaagtc cgaaatcttt gatttcgaag acgcacccac gtcagggcga ctaggtgctt
     2941 tagcagcaga cgcctacgac gcaaaaatct gacgaagatg ctttagcatc taggaagatt
     3001 tatctttttc actagaaaat tagtcccgtg ttcagttcct ttcttctttc tttttggagc
     3061 gaccttcggt cgctgattgc tgacgctaat atttgaatga gtagtcttat tacttaatag
     3121 ctttgcttcc gatgtcgttt cggtagaaga ggcctgtggt gtcttgttgg gctagttggg
     3181 tatagatttt gtcctgccct gctttgacgc tgtcttctgt ggtgacaagc atatagactc
     3241 gtccaccgtt tgagagcagt gctttgccat tttccgcaaa cttagctccg gcatagtagg
     3301 tgatgatgtc gccgtcggtc ttttcaggca gtggcacacc tttctcgtaa tcgagcggat
     3361 agccttctga ggcaacgaca acgcccaaag tcacgccgtc cttctgccaa gtgatgtaag
     3421 gctcgatgcc catcatgatg tcgtcgatgt tctgagcaaa atcagaagtc aggcgaggga
     3481 ggataatctg agtttctggg tcaccgaaac gtgagttgaa ctcgataacc ttcggaccat
     3541 cagccgtcag gataagcccc gcatagagga cgcctagata aggacgccct tcagtaatca
     3601 taccctcaag gataggctta acgatggttt caaccgccgt atccactacg ctctgaggca
     3661 ggtggggaac tggcgcatag gcacccatac cgccagtgtt tggtcccata tcaccgtcat
     3721 aggcacactt gtgatcctga gctgtcggca tgatgtaaaa cttgtcgcca ttagcgaagg
     3781 caaagaggga gaactcttca ccatccaaaa attcctcgat aaccacacgc gctcctgagt
     3841 cgccaaactt gttgtccaaa agcatctctt gcgccgcctc taccgcctgc tcaacggttt
     3901 cagccacgac cacgcccttg cctaacgcca agccatcagc cttgaccacg attggtgcac
     3961 cctgctcttc gatgtaggct ttagcttttt caaaatctga atatgtgcca taggctgctg
     4021 ttggaacgtt gtatttaacc atgatttcct tagcaaagtc ttttgaccac tctagctccg
     4081 ctgctgcctt tgttggccca aaagctctaa gtccagcact gttgaaatca tcaacgatac
     4141 cagccgctag cgcatcatca ggaccaataa aagtccaagc aatctcattc tccttagcaa
     4201 agtcaatcag cctagaatgt tcggaaatta cgatatttac caagcctaga ccatccaagg
     4261 tcataccatc attaccaggt gccacaaaaa cctgatctac acccttagac actaacaact
     4321 tcttagcaat cgcatgctca cgaccaccag aaccaacaac aagcaatttc atatcagaat
     4381 aactcctatt aaataatata aaaagaaatt tgcaataaaa acgtattatt tataaaatta
     4441 taacacattt gttcgctttt aagttttttg ttgcatcttt tttgaaaaat taataacata
     4501 atatatactt cacatatatt atgttattaa tccttcatat tgctatttaa aacttatcat
     4561 tgctgtttct tagaacttag tttggcccct cgctaacttt atgttatact gaatatgtta
     4621 agcgctttct taaagaggag aggaccgtgc attatgacta tttatgacca aattgagtct
     4681 gctcttgatt taatgactga tttagaacat gaaattgctt gttattttat ggggcagccc
     4741 atttcaaaag acgctcttgc ttcttccatt gtcactaagc aactacacat ttcacaagca
     4801 gccttaacgc gttttgctaa aaaatgtggg tttaaaggct atcgtgaatt tgtttttgaa
     4861 tatttaaaaa gccatgagac catcagtcaa caattatacg gtcttcaaaa tgacaacact
     4921 aagaaagtat ttatgaatta tcaagagatg gtttctaaat ccgctgatat tattgatgaa
     4981 gaacagcttc tagaagtatc acatatgatt gagcaagctg atcgtgttta tttttatggc
     5041 aaagggagct catcgctagt agctaaagaa tttaaaatac gccttatgag gctgggagtg
     5101 atttgtgagg ctctggatga tacagatagt ttctcatgga ctaatagtat tgttaatgat
     5161 agatgcctcg tcattgcctt ctcactttct gggaacacca actctgttat cggtgcctta
     5221 aaaatcgcaa gttgtcacgg agctaaaacc gttctattta caaaacaacc tcacacaatt
     5281 gactatgctt ttgacaagat tattcaggtg gcctctgcta ggcatttaga ctacggcaat
     5341 agaatttcgc ctcagatccc aatgttaatc atggtagata ttatctatgc acaattttta
     5401 gatattaata aaatagaaaa agagcgaatt tttagagaaa ccattattca acgctaaatg
     5461 aaaatcgagt agaaataatc tactcgattt tttgctttgt aaattgactt cctgtcagtt
     5521 gatcaaaaat atagtctttt acttgtactg tcattggatc atgtccatag tctggtaata
     5581 aaagatgctt tttagttgat gtcaaacggt taaaaatggc aaattgactt gatggaggac
     5641 aaatatcatc tttcaaggct gttagtaaaa caacggggca agaaatccta tgtgcaaaat
     5701 ttttaacatc aatataagcc aaagttttta acacattatt ttctgtttta tgaaaaggat
     5761 cactatattt aaaatagcgg aagagttcgt cataaggctc actgacattc cctaaatcta
     5821 atactcgtct aaagtctgac aaaaatgggt aaacagcaac ggtcttgacg attttgggat
     5881 taagagctgc agctatcaaa gcaagtgctc caccttgaga ccagccataa ctataaagtt
     5941 gattactatc aacactctct aatgtagcaa tgatatcaat caattggaaa acatcaagat
     6001 aaatgtcttt ataaaaaaga tgatttggtc ctgagatcat tcctcgcaca atctggccct
     6061 taactgtaat gccatcaaaa tgccccttgt cttgagactg tccagcttga cctcgaacat
     6121 ccattgaaac aacgccgtaa cctgctgcta cataattaag ttggtcactc cagtcaggac
     6181 tttggttttg gtaaccatga aaataaaaaa ctacaggata gggtttattt gttttaggga
     6241 ataaacattt ggcatagaca atagagttat tgctgccttt aaaatgaaga tcataagctt
     6301 gtgcaaaagt aatattgaaa tcttttttgt ctagacgata ctcaatattc ccttgatatt
     6361 tcattgtttg ttttttccag aagtcatcaa aatcctcggg aatttggtct tgacctaggt
     6421 attctctcat gtcatctagc gacattgttt caatcatata cccgtcaccc cctgttttta
     6481 aaatggtagt aagctcctag cataccagct tggttttcgt gttgtgcaaa aactattatt
     6541 gttttttcag caagacttgt cacaagattt cgtttcaaag attcactaag tttatcttgt
     6601 aaataatctt tttgagccat gattccacca cctaaaacaa ctttttcagg atttaccaca
     6661 taaaccatat tagcgattcc ttgaccaagg tagtttatca tacggtctat acttgcaata
     6721 catttctcat tgccttcttt agcctcctga aaaatacgac gtccatccca tcggttaatt
     6781 tcatctccat gcgctttggc aacatcagca atcagtgcag ttgtagaggc taaatcttga
     6841 aaatcaccat ctgaaaggtg catatagcca acttcacaag cagaattact aaagccatga
     6901 aaaactgttt tatcaataat cagacaacca ccaatacctg ttccaatagt aagacaaagc
     6961 gctattgaac tatctttggc actacctgag acagcttccg ctaatcctgc acaattaaca
     7021 tcattttcaa tttctgtgcg tacttgataa gtgtcttcta aaactttctt aaattgtgtc
     7081 ccagcatagt ttggaatttg aggaccagag taaaatatgc atccttcatc tggatctacc
     7141 atgccagcag atgagatagc aattccatca atgctccctt ctgttagata gttatcgata
     7201 atttgacaaa ctttttgtaa aatgcctgga ccacctttgt aggcttctgt ggcaagctca
     7261 cttgcctcaa caatacaacc taaattatcc acaatgccat gcttaatcat tgtaccgcca
     7321 atatcaattg ctactgttct ggtcataatt atctacctca ctttaacaaa actgtttttt
     7381 agcatgtcta atcatttctg ctgcctcttt aataataggt ttatcttctt ctgttacaga
     7441 tgctaaaggt aaccgaacgg aacctatagt tagttgctca ttgatttcca atacagcttt
     7501 gatcacagca tacatattac catgacccga acaaagcttt gtgataatat cgttaattgt
     7561 aaattgaagt tcacgcgctt tttctaaatc tttatcaaca attaactgat ttaaagtcaa
     7621 gtataattct ggcattacgc cataggtacc accaatacct gcagctgctc ccatgagacg
     7681 accacccaaa aattgttcat ctggaccatt aaaaacaata tgattttctc ctccaatagc
     7741 cacaaaattc tgaatgtctt gaactggcat ggaagaattt ttaacaccaa ttacttgcgg
     7801 attttgtaac atttttcgat ataaatccga tgtcaaagca acccctgcca attgaggaat
     7861 attgtaaata ataaaatcag tttgtggtgc tgcttgacta atagtattcc aataatctgc
     7921 tatggcatac tctggtaatc gaaaataaat tggtggaatt gctgcaatag catcaacacc
     7981 catagcttca gcatgcatag ccaactcgac gctatcctta gtattattac atgctacatg
     8041 cgctatcact gttagtttac ccttagcaac ggacataaca ttttccagta caagtttacg
     8101 atctgctaca ctttggtaaa tacattctcc tgatgaacca ttaacataaa gtccttgaac
     8161 acctttatca ataaaataat tggtcagggc ttttactcgt tcgggacaaa tatcaccctt
     8221 atcatcatag caagcataaa atgcaggaat aattccttga tatttttgta aatctttcat
     8281 tgataatccc cttgttgttt gctgaaatag gcaaattgtt tttgtaaaat aataatttta
     8341 aagtaagcaa aggcggtata agcaaaaata aatatagctg acactgtaag cataaataag
     8401 agactagact gcaccataat aaaccaagtc atgattaata aaaacatcaa tacaccagtc
     8461 catttcaagt ttaaacatgc cattataaag ctctttttca cggtatctaa aagagacatt
     8521 tcatagcggg cagccatagg aaataggtat atgctcgtca tcacagtcaa taacaaaaag
     8581 gcgtaacaaa ttatcttcaa gaaaggcaca atgaaaccta tttgataatg aagaattgtt
     8641 aaatcaaata tgatcacaac caaaatactt agttcaacca aacctaaaag cataccttgg
     8701 aaccactctt tttttaggtg ttttatatac aatattaaaa gatttgtctg atcaccttct
     8761 aatttttgcc acaacgttcg atacaaacta attttagcag caccaatagt taccaatggt
     8821 aggcaagtta tcataaagag aaggtttagc aatgccagat caaaaacaac attgcaacct
     8881 tgcataaagg ggttgttaag atcaaaaatc gctgctatta attgatttgc ttttttcaca
     8941 atatactccc tgtatttctt acgccattaa cactttcaat accccttttt tgacataatt
     9001 acctagactt cccataccat ttggctgatg agcttcttct gggaaaaaga aggctatata
     9061 attatctctc aaatcaaaag aagtctcttc tgagcacttg acaaatccta tatcgcctaa
     9121 gtattcctct acctctacct tatcaccttt tcccaatttg ataacttcat atccttcaat
     9181 gacataatgt atatcaaggt attttttatg gtattcaaaa atatgatcaa atgactcact
     9241 aagttgattt gattggacca tgagtatcac ttctgggcta atatgatagg tgcctatatc
     9301 taaattcctt aaatcatgac ttagtagata atcaatcgct aaatctaaat taggattaat
     9361 atctttataa tgcgttaaat tcaataaatg atcgtaaatc ataataaatc ctacctatcc
     9421 tttaacagct cccattgtaa tcccctgagt aaaggatttt tggaaaacta agaatactgt
     9481 cacaatagga acagcagcta aggctgcacc tgccataatt aagccatagt ttgttgccat
     9541 ttcagcctgc atagtagcta ccccaagaga aatggtcaga ttatttctag aagttagcat
     9601 aactaattgc ataaaatagt cattccaagt attgataaat gtgaaaatag ctagggcagc
     9661 aaatccaggt ttgacaattg gaaatgctac attaataaat gttcgaatct ctccacaacc
     9721 atcaatttta gctgattcca gtaattctgt tggaatattt tcagaaaact gtttcatcaa
     9781 gaagacccca aaaggccaac caacaagagg taaaataacc gcccataagg tatcatgtat
     9841 ccccataaaa ttgataattc taactaatgg cactaaaaca acttgtttag gtaaagccat
     9901 agcagcaata aaaagggaga acaatatttt ttgaccgtaa aatcgtttct tagctaaaac
     9961 atagcctgcc attgacgatg tacagcaaac taggaacatt gtcataattg agataaagac
    10021 actattccaa agccatctca aagctgggtt ttgtacagtt aaagctttaa aattttctaa
    10081 ggtcggtgct ttgggccacc attgtggggg gatgataata gtatcaggtt gagatttaaa
    10141 agctcctgtc ataatccaat aaaatggaaa gataaaaagc actgtcaaaa gacaaaggat
    10201 aagagccgtt aaaaagttat aagcactgaa tgttttcttt ttcatgtttt cctcctaata
    10261 ttccacatca tttcctaaga tcttaaattg agcaaaactt attaaagcaa tcattactgc
    10321 taggaatact cccatagtat tagcatagcc atattctgat agtttaaagg ctttttcata
    10381 aagataatac atcaaagtgc ttgttgaata atttggtcca ccagatgtta ggagctggat
    10441 taaggcaaaa cattgaaatg aattaattgt tgttataact gcaatataga gtgtagttgg
    10501 taagagactc ggccatttaa tttgccaaaa aacttgcatt tcattggctc catcaacacg
    10561 cgcagcttcg caaagagaat tatcaatatt tcccatcgcc gcaatatata gaataatagg
    10621 ttgaccaact gaggtcgtta gcaaaataat aataatagca agtaatgccc agtgcttatc
    10681 accaagccag ctaatattct gttcaatgac atgacctgat ttcaagatgt aatttaaaat
    10741 tccggacata gggtcataaa tccatttcca aacaactgtg actgcaacac taccagttac
    10801 tactggcaag aagaagacac aacggtagaa agagcgtgaa aaaacatttt tttcataagt
    10861 attggcagca acaaaaagtg agaagaaaac cactactggc acagagccaa tcacaatgat
    10921 tactgtattt atcaaggatt tcatgaaaat agaatcatga aacatacgat tgtaattagc
    10981 taacccaata aaagtgaact gtgtcataga ataattaaaa aaactagtga caaagcccat
    11041 aaccatcggt gcaaaaacaa aaattaagaa aaataagaga ataggcgcta gaaaagcata
    11101 agctatcatc gtttccctca tctttaactt attagtacgc atacggtact ctcctttaca
    11161 tcttaacttg actaaaaact cctcttctat cccaatatgt gattgaccat agaaaaggag
    11221 tctccttaat tatttagctg cttttttaat ggtatcattt gctttttgag taaagtcttt
    11281 caaagcatct gctggttttt catcaccatt ggatacagat tgaaccattg ggaaccataa
    11341 ggttctcatt tcagaaaatc catcgatagt gttgtaatat ggtgaataat attgagtcca
    11401 ttttgaaatc ttcatcatac gtttatcacc tttataaaga tccccaaatg atgttctaac
    11461 tgggaaagca cctgtacgta taacatcttt tggtccccat tttttatcat cagcaataaa
    11521 agtgataaat ttcttagagg cttttacttt gttttcatct ttattattaa agaccgcaaa
    11581 accattaaca aggtattcta aatctggttt tccatcttct gatgggaatg gcacttcaag
    11641 gtaatccact ttacttgact ctaataattt tgcttgagtt tttggttgag ctggagccca
    11701 taggatagtg aaagcagttt gtccattggc gaagttttga atgtcagctg agccatcata
    11761 ctgagaccca ttcatcaagt agcctttctt aatccattca actatttttt tcattgattt
    11821 tacagaattt ttagtgtcag tggtatattt tgttacttct ttatctgtta ttggagcact
    11881 ataaagatta gcaaaaaatg cacgtggtcc ttgatctcct ccttgcccgt ttgcaaagaa
    11941 tgaacctggt gtatagcctt tattttttag tgcttttagt actttttcaa aatcactagt
    12001 agtccaacct tcttttacaa gtttcaaaac tcctgcatct ttaagcattt ttttattgaa
    12061 cgccatataa aatggggcag aacttattgg atacatgtag gctttatcgc cagacttaga
    12121 agcttgaatg atgttcttat tattgacatc cttaataaat tggtctgtaa ataaatcatt
    12181 caaatctgct aatttaccat ttttaccata ttgaataatt cgccctggtg catcaaaaag
    12241 cacatcaggt gctgtccctg cttcaattgc tgtagtgatt ttttcaggtc cagatgtgaa
    12301 atcaattgtc tctagtttta cttttatatt aggatttttc ttttcaaaag ccttgatgac
    12361 ttttttctca taagtaccta ctccatcctt agccttttct tgagtaaagg ttggaaaagc
    12421 ccaccacgta atttcgactt tatccttctc ggcagccata acagattgac tactgtctac
    12481 acaagcaaac attgataatg ctgcagctcc gaggcaaaaa ccaatcacac tttttttgat
    12541 actcataact attccccttt tttattttta ttttgatttt tgagtaacac ttttaactaa
    12601 gtcctgagat gaaacgctcc gctatttctt ttggtctagt gatagcaccc ccaactacaa
    12661 ttcctgcaac acctatatga ttaatttcat tagcctgctt aggagtatga attttacctt
    12721 ccgcaatcac atctataccg gcttgacaaa gcttattaag gagttctata tccggtcctt
    12781 cttcttggcg gctgtaatct gtgtatcccg atagagttgt acccacaaaa tcaactcctg
    12841 cttcaaaagc atttttgcct tcttcaaaag tacttatatc agccattaac aactgtttag
    12901 gatatttcct ttttatcttt tgaataaact cagctacact caaaccatca tgacgctctc
    12961 taagtgtaca atctaaggct attactgcaa tatctaaagt agctaattga tccacctctg
    13021 tcatcgtagc agtgataaat ggttcttgtg gaggatattc acgtttaata atgccgatga
    13081 taggtaaatt agtaacttct tgaatttcct taatgtcgcg gacactattg gctcttatac
    13141 caaccgctcc tgcttcttga gctgccaaag ctaaaagagg cataacacct ccactttcag
    13201 tataaagagg ctccccaggc aaagcttgac atgacacaat aatgccattt tttatttgct
    13261 ttttaaaagc ttctttactt agatgtggca tattctcact tccttatctt ttttaatttt
    13321 agatatctac aaaccttatt atatctattt ttaaagcgtt ttcaataatc tttacagact
    13381 tagattttag ttttaacgtt ttgatttagt cacggttaac gtgaaagtag tttcagactt
    13441 atagcttaat ctcctcaatt taataaaaat tatataagaa ccttgaaagt ctattcaagg
    13501 catataaaaa agaagccaaa tagcttcctt ttttatatta tttgttaaat gatacgtgaa
    13561 cgtggtcata gtggttggca gtaacgccac cacgatctgg cattgcattc caagtattag
    13621 caggtccata aatactattt gtatttgaat aaaacttttg ttgccagata acatatgaaa
    13681 tgttatttgc tgccatattt tgtgtagagt actgtgcaac ttcattacca agtgcttggt
    13741 ttttacctac aataaagtca actgctaaac ctttaccatg atcacctgga tctcccgcac
    13801 ggtatgtact gaattcatta actccataag ttgacgctac tttttcttta taagctgcaa
    13861 catgaggttg gagccctgca ttttcaggat gtgcagctac tgcatttgtt gttgaagctg
    13921 gttgtgctac cggtgttgct gttggagctt tttgtgctac cggaacgctc ttaacttcag
    13981 tcgcttgtaa cttactgtct gtagctggtg aagtcgtagt cacaggaact gctggagctg
    14041 atacatgctc tggtgatgca ccagtttcta ctttaggagt gactacttta gcacttgcca
    14101 ctctaggggc tgctacagtt cttaccggtg ctactttagc tactggagct ggtgtttcag
    14161 cggcaacaga agctggtgat actgttgtta actgactgac tgacgtctga gttggtttaa
    14221 cttcctcttt agctgctgga acttctgaag taatcgactt cacaggagct ggtgatacct
    14281 gttcattagc tgctacttga ctaacagctt gctcttgtgc taatacttct tttgatttca
    14341 aagctggcgc agaagaatat gtcttcattg gcgaaacaat cgttgttgct gcttctggtg
    14401 tcataccttc cgaaattgta ttgagagaaa ctttttggtc tgcaacaaaa acttgattgg
    14461 ttttcaaatc gacagtagct gttgtttgac cagcagcatt tgttgctggt gtttctattt
    14521 tcattgaagt ggcagtatga ctcttctgat cgtaagttac tgtcagtgtt gtctcaggat
    14581 aaataagatt gatatctgca atgttattaa tttttgctaa gacattcata tcaattgaca
    14641 ttgcttctga aataacgctt agtgtatcac catatttcac agtatatgat gatttattgt
    14701 cttgctttac caaatcagcc tttacctctg aaacagtacg tgctgtccac gtcgtatctg
    14761 tttcttgtgc ttgaacactt gcgactgata atagcgaagc tgccattgtc gatgtcaata
    14821 gtaccttttt attcattttc attgcttcaa ttcctatttc ctttttatta gtccgagact
    14881 ataatatcac actttactgg actataaaat tattttcaga cggtttaaag aaattgtact
    14941 tagttagtaa caaaactgtc atttaaaaca atgaatcaat acctcaaact atttgtaaag
    15001 cagttcttga ctgttgtcaa cgcgaagcca aactgaacta ccattagcaa agcgtgtctt
    15061 agcccaagtt aaaccagctg ttccttggat gggtttttct acagtcttaa gagttttagg
    15121 attaaagata aagtaaccac ctttttgaag aacctggtca gctgtcaaat taccattagc
    15181 atcaacctca tcaatttctg atgctggtat accattatcg ttccaatcaa acccagtcgg
    15241 cgttagggtg ttatttttga ctaaccaaac accattaacc ttttgtaatt catctactcg
    15301 atacactttt aatttactag attgattctg tacaggagct gattgtaatg gcttaatgct
    15361 tggagctgat gcttgcgttt ttccactaaa ggtcgcaacg ttagcaatta gtgacgttgg
    15421 attgatacgt ccatggaaac cattttgaaa attagggtta gctggtaaaa attcaaaatg
    15481 aaggtgaggt cccgtcgcca taccagttgc tcctacgtaa ccgatgatat ctccttgttt
    15541 gactttttcc ccagtcctag ccaccacacg tgacatatga gcgtaaccac tatgcattcc
    15601 atccgcatgt tgaatcatga cacaatttcc tgctaagtct gtcatccaag aaaagttggc
    15661 tccagctcct gcaaatttca cagtaccatc tgccactgcc ctaataatcg ttccagtcgg
    15721 aacagcataa tccaccccac aatgtccagg ataaccattg aaacctgttg taattctacc
    15781 attatcaatt ggacggacat aagtatccgc taaaactcgg gaacccgcag ataaaaccat
    15841 accacctaaa acaactaagg aacttgcctt aactaaccat ttattcaaga aaaacctcca
    15901 aatagaatta atataatgaa gtgctcaaac acttgcaatt aacttattcg aaaatttaaa
    15961 tcaaaatatc aaaaaaagta ctcaccttga tgagtactta ggaggtttaa taaaaagaaa
    16021 agtttttagg atatacgtag tatacctgag ttaacttaag aaaacctaaa ctactatttt
    16081 ttaatgtcta aaatgtctca cgcctgtgaa gatcatggtc aagccatgtt tgtttgcggc
    16141 gtcaatagat tcttgatcac gaactgaacc acctggctgg atgattgctt tgatgcctgc
    16201 ggcagctatt tcttcaatgt tgtccgcaaa tgggaagaag gcatctgatg ctagaacggc
    16261 accgtcaagg tggtccttag cctgctcgat agcaatcttg actgagccga cacggttggt
    16321 ttgacctaca cccagtccaa gcgtcatgtg atcgttagta ataataatcc cgttagactt
    16381 aacatacttg atagccttcc aggcaaactc aagggcagtc acctcttgtt ctgttggctg
    16441 gcggtctgtc accacttgcc agtcagatgg attttcagcc acaacgtctt ggttttgcac
    16501 caaaagtcca ccaactacgc cagtgtactc agcttccact tcgctggcag cttgggcatc
    16561 aaacggcaac tcaaggatac gcaagttttt ctttttattt gtgagaatag ctagcgcttc
    16621 ttctgagtat gatggtgcga tgatgatttc caagaagata gggtgcatct tctcagctgt
    16681 cgctgcgtca acttcacggt taaggacaac aattccacca aagattgaaa ctggatcagc
    16741 ttcataagcg taatcccaag ctgtctcaat atcatcagcc tgtccgatac cacatgggtt
    16801 catgtgtttg agggcaacaa ccgttggact gtctttgaaa tcgcggataa tacggattgc
    16861 tgcatcagca tcacggatat tattgaagga caattctttt ccattgagct gtttagctga
    16921 agcgattgag tagtctgttg gtaaggcttt ttggtagaaa tcagcgtctt gctgtggatt
    16981 ttctccgtaa cgcatagcct gtttaagatc ataagtgatg gtcaattttt caggcttagc
    17041 ctctcccact tgagctgtga agtactcagc aatcaaagcg tcgtaggctg ccgtatgacg
    17101 gaaggcctta gctgccaagc gttgacgagt tttaaatgtc gtctgactag cgtcagccaa
    17161 ttctcccaaa acagtggcat agtcagctgg atcaaccaca acggttacgc tagcgtggtt
    17221 tttagcagct gagcgaagca ttgatggacc gccgatgtcg atgttttcca ccgccaaatc
    17281 gttggtcacg tctgggcgaa ggatagtctc cttgaaggga tagaggttga caaccacgag
    17341 gtcaatcaac tcgatgttgt tgtccttagc agcctgaagg tggctgtcag cgtcgcgacg
    17401 agccaaaatc ccaccgtgaa tgtttgagtg gagggtctta acacgaccat ccatcatttc
    17461 tggggagcca gttacatcgt caatagcaat ggtctcaaca ccagcatcgt caagggcaac
    17521 cttagtccca ccagttgaga taatatccca acccaagttt ttcaattctt ttgcaaagtc
    17581 aataattcct gatttgtcag aaactgagat taaagcacgt ttagtcatga gttctccttt
    17641 tcttacttca atctatttca taggctatgt attcatgaga aaattcataa ccgagttttt
    17701 cagataaatt caatgaagtc cttgtatgag catcccagct aggataaatt cccttatcta
    17761 aacaagttag tatcaactga gctgcaacta ttgttgctaa accacgccga cgaaaatctg
    17821 gatgcgtatc tacttctatc tcgatcccat ttttataggt tgaataagat gaagctcctg
    17881 caatgatatt cccctgataa tatataacat aacctatacc ttgtttttta tagtactgat
    17941 aagtagcgta atttgctact aaatcctgtg accattcctt ttctaagcaa gagttgtaaa
    18001 ctttctcatc aataacacgt aattcaaaac catttggcaa ctgagtaaca aacttctcta
    18061 acctacttcg ctcaaacaaa gtatcttttt tcgtcgcata acgcttaaaa gaatgggcat
    18121 tctggccata agttgattca atcaaatctg accatccttt atgctgagga acaaggataa
    18181 tatcctcccc agagcaaact tctagtagaa ataaagtagg ctgacctgct agaaagccaa
    18241 aagatgattt ccttcccaat tttgccaaag aagattttgg ttggtctaaa ctatctacaa
    18301 aaacttctcc cataatacct tggacacacg accagattat ggtctcgtcc caatcaccaa
    18361 aaatagactc tactatctct aaatttttca ctagtttcat tagcttatta taacaaattt
    18421 tactattttc tctttatgcc caaactatcc aaaacatctg gatagagttg atattcggtt
    18481 tcatggatgc gggtttcaaa gctttctagg ctatcgtctg ctaggcgtgg cacgcgcact
    18541 tgttggatga cctgaccggt atccacacca gagtccaccc aatggatggt cacaccagac
    18601 tggtcaacac ctgcctccca agcatccttg ataccgtgag cacctggaaa ttcaggcaga
    18661 taggctgggt gaatattgat gatacgccct tcataggctg agagcaaggt ttctccgaca
    18721 atcttcatgt agcccgccag acagaccaag tcaatctcgt gtttgtccaa caaatcaacg
    18781 acggcttgct cgtaagccgc cttattctca aactctttga gttcaaaagc gaagcttgga
    18841 atggctaagt tctgagcacg ctctaaaaca taggcatcac gatgatctga aaagacaaaa
    18901 ctaactggaa actgctctgc tatgacctga aagttggaac cattagcaga agcaaaaaca
    18961 gcgattttca tattcgatac aaagggatga ttcggcttgc cgaaaaattt ctagaaaaat
    19021 aggaaaacga acaacgacgc ttgcgtctaa gtcgttttat cttttttccc agaaatttaa
    19081 tcccgtgttc agttaggcaa actaactaaa cactcctttc ttataaaatt tagtgactta
    19141 tttaatcacc acactatcgt ctgctttctt gatgatacga ccgatttcat aaactggttc
    19201 gtccaagagc tctttgacgc ggttaacatt ttcaggacta acagctagca taagaccgac
    19261 acccatattg aagatttcaa acatttcttc gtgcttgatg tcaccatatt tttcaagcgc
    19321 cttgaaaatc ggaagtactg gcaccttatc ctcatcgatt tccgcagcca aatcatccgc
    19381 aaacatacga ggaacattct cgataaagcc accacccgtg atgtggccga taccgttgac
    19441 tagttcttcc ttgattaatg gcagagctgc ttgaacatag atacgagttg gctcaagaag
    19501 gacatccttg agttgtttac cttcaagctc tggaagcagc tctttaccag tatagtcagc
    19561 aaagacacga cgcaccagcg aataaccgtt tgagtggata ccacttgaag caagtcccaa
    19621 aagaatatcg ccttccttta ctttggaacc atcaatgagc tgagattttt cagccacacc
    19681 aacagcaaag ccagcaaggt cataatcatc ttcgccatac atcccaggca tttcagcggt
    19741 ctcacctcca atgagtgctg ctcctgcttg aacacaacct tctgcaacgc cagaaacaac
    19801 ttcttcaagt ttgactgggt tattttttcc tgtcgcaata taatctaaaa aatataatgg
    19861 ctcagcacct gctgcaataa tatcattgac acacatggca acacaatctt ggcctatggt
    19921 gtcatgctgt tgatatttaa tcgcaagcat gagttttgtg ccaacaccgt ctgttcctga
    19981 aaccaaaata ggctctctaa ctcctgtttt actcaagtca aacaccccac caaagccacc
    20041 tagagctccc atgactcccg cacgttctgt acgagaaacg tgttttttta ttctttcaac
    20101 cacttcataa ccagcttcaa catcaacacc agattttgca taagcatttt tttcagacat
    20161 gttttctcct tatataatta gcagagctta tttcactttt tgaatataaa aacttgtttc
    20221 ttcttctaaa cttctgaggt attcttcttc ataatcataa agcggtgttg gataatgtcc
    20281 atcaaagtaa gctacgcata aaccaccatt tggtgctttt gtttcgagtc caattgattc
    20341 aatcaaccca tctagtgaga gataggtcag actatctgcg ccaataatat cacacacttc
    20401 gtcagcagcg tgatttgctg aaattaattc tcgtcttgtt tgaatatcaa taccataaaa
    20461 acaaggatac tttaattctg gactagctat agcaacatgt acttcactag ctcctgcttc
    20521 tcttaataat ccgacaatcc ttctagaagt cgttcctctt acaattgagt catcaatcat
    20581 aacaacgcgc tttcctttga caacaccaga taccgctgat agtttcattc gaacaccttg
    20641 ttcccttaat tcttgtgtcg gttgaataaa ggttcgctgc gtatactgat tttttacaag
    20701 acccatctca tttggtaatc cggattcttc agcaaagccc atagcagccg ataaggacga
    20761 atttgggaca ccaattacaa tatcagcatc ctgtttaaat tcttgtgcaa gacgctttcc
    20821 catatttttt cgagccgtat gaacattaac accatgtata gttgaatccg gccttgcaaa
    20881 atagacatat tccatcgaac aaattgccag ttgcgtttca tcagtataac gatcacattg
    20941 aataccacta tcgtctataa ggataacttc gccaggttca acatctctaa cccattttgc
    21001 gcctaccacc tcaaaagcac aggtctcact ggaaataacc caagcaccat tttgcatttg
    21061 tccaattgac aaaggacgaa aggcattagg gtcaagagca gcaattaatt tatcttctgt
    21121 catcagtaga taggcgaagc ctccctttac agtgcttaaa gcttctttta ccttccccat
    21181 aaaacttggg ttatggcttc gacgaatcaa gtgcatcaaa atttcagtat ctgaggaggc
    21241 attgaaaatt gcaccttgct tttctaattc tttccttaaa gaaatagcat ttgtcaaatt
    21301 accattatgg cataaagcaa attgcccgtc atgaaattta taaagaaaag gctgaatatt
    21361 gcgaatatct gcagaacctg cagtagcata ccgaacatgt ccaatagccg cattcccagt
    21421 taaattatct aattcagatt gattcttaaa aacttcagaa aggagcccaa catttcgata
    21481 accatagagt ttcccattat cattcgaaac aataccagca ccttcttgac cgcgatgttg
    21541 aaggctatga agcccaaagt aagtgacttg agctgcctga ggatgccccc agataccaaa
    21601 gactccacat tcttcattta gagattttac ttcgtatgtc attctttttt cctaattctt
    21661 tttgtatctc atcttaagta gctttgatga ttttctcaat tttggatcta tcgtcaccaa
    21721 tgccaacatc aaaatagaaa atctatcaag ctattttcaa gctaatcatt gagtcttagc
    21781 ctcaacggct cccaaatacc actacgacag aaagatacag ttaagctcgc ttcgctcttg
    21841 tttccttatc tgttttatga aaaaatttga aaaaattatt ttcctgtaaa atattttact
    21901 gcactagcaa acaaggcttg gtctttgtta ccagggatat tttggaagag gccgtcttcc
    21961 cagcgttctg agtgccccat cttaccgatg atttgaccat tcttgctggt aatcccttcg
    22021 atggcattga cagagccatt tggattgtat ttagaatcca tagatggttg tccgtcaaag
    22081 tccacatatt ggctccagat ttgaccattg tctcttagct ctgcaaattc agaagcgctg
    22141 acaacaaatt taccttcacc gtgtgaaact gggatggcat gaatatcgcc gacctcaact
    22201 cctaccaacc aaggtgagtt ggtatttgcg atacgagtct caaccatctt ggcaacgtgc
    22261 tggttggcat cgttatagaa gagggttgga cttgtctcac cagcttcctc gaagtttccg
    22321 tatggaagaa gacctgattt aacaagggct tggaaaccat tacagatacc gatgataagg
    22381 ccaccttttt cgatgaagct gtcaatagct gcgcgaacct tcttgttaag caagatattg
    22441 acgataaact tagcagaccc atctggttca tccgctgctg agaatcctcc agcaaagaag
    22501 atgatatttg ccttagcaat attagcgacc attgtgtcaa ctgagtcagc aatagcagcc
    22561 tcattcaagg ttacaaatgg taccaagttg acactagctc caacctgttc aaaggcctta
    22621 gctgaatcat attctgagtt ggtaccaggg aagactggaa tgtaaaccac tggtttttca
    22681 attgtttcct tagccttgat aacagtatct gataccacag caggaacttc ttcaagagcg
    22741 tctgcctgtt caaactctgt tgggtaaacc tcttccaatt tgccttcgaa gactgataga
    22801 aggcttgccc cagcaaggtc atttccattg acagtaagtg taaaggctgc ctgagtttga
    22861 ccgattttca ccgagtcagc aatttcctca gctgatgtaa agacaaagcc tccgagttga
    22921 gctgtcaagc tactgtcaag ctctgcaatt tcaacagagg ccccgatacg gttaccaaaa
    22981 gtcatgagag caagactttc taggacacca ccgtatttaa cagctgaagc agcagtaatc
    23041 ttatgttgag cttgaatagt ctcgaactgg ctaaagttag ccttgataag gtcaaaatca
    23101 atatcttctg aaatagcttg acctggaatg tagtagatat tctcaccagc cgctttaaac
    23161 tcaggagaga gaaccttgcg gctgtccgct gttgtcacac cgaaagctac caaggttggc
    23221 ggtactgtca attcttcgaa agtaccagac atagagtcct taccgccgat tgatggcaat
    23281 ccaagttgaa tctgagcctc aatagaacca agaagagctg atactggctg atcaaaacgc
    23341 tctgcctgtt tatccatacg ctcgaaatac tcttggtaag agaaacgtgc acgagaccag
    23401 tcagcacccg ttgctaccaa gcgagctgtc gcttcaatga cagcataggc agcaccgtgg
    23461 taaggtgacc attctgcaat ataaggattg taaccttgag ccataacaga tgcagttgtt
    23521 gtgacaccgt gttgaactgg caatttttga acagagcttt ctgttggtgt gatttggtaa
    23581 cggccaccga ttgggtggtt aacggttgag cgaccaacag atgagtcaaa gatagtttgg
    23641 agaccttttt gactagcgtg gttgaggtca gacaagacct taagcgtatc cgcttcaagt
    23701 gtctctgctg atgttgtgcg tacctctgga actgtcaagt ctttgtcaac gactttagca
    23761 tcaacaacga cacggacacc gttggtatca aggaaacggc gttccaagtc aacaattgtt
    23821 tcaccatccc aagtcatgac aaggtttggt tttgcagtaa tagtagcaac cacaacggca
    23881 tcgatatttt ccttgttgca ggctgcgatg aaggtatcca cgtcactagg acgaacaacg
    23941 actgacatac gctcttgtga ttctgagatt gcaatttcag taccattaag accttggtat
    24001 ttaagcggta ccttgtccaa atcgatttca agaccgtcag ccaattcacc gatggcaaca
    24061 cagacaccac ctgcaccgaa gtcatttgat ttcttgatta ggcgagtgac attaccatca
    24121 cggaaaaggc gttgaatctt acgttcttcg atggcattcc ctttttgtac ctctgcgcca
    24181 gctgtttcca cagattcaac cgtttgaacc ttagatgaac ctgtcgcacc gccgacaccg
    24241 tcacgacctg ttttaccacc gagcaagatg accacatcgc ctgcttctgg tttttcacga
    24301 accacatttt ccttaggtgc agcaccaacc acagctccaa gctccatgcg tttggctaca
    24361 aagccagggt gaaagtactc acgcacataa gttgtcgcaa gcccaatctg gttaccatat
    24421 gaagaatagc cgtgcgccgc agttttagaa ataacctgtt gcggcaattt accagcacgt
    24481 gtttccgcaa tcggagtcgt aatatcgcct gcgcctgaaa tacgcatagc ctgataaacg
    24541 tatgaacgtc ctgacaacgg gtcacgaatg gcaccaccga tacaagttgc tgcgccacca
    24601 aatggctcaa tttctgttgg gtgattgtga gtctcattct taaacatgag gagccaaggc
    24661 tcttttacac catcaacatc tacttcaatt tcaactgagc aggcattgat ttcatctgag
    24721 acttccatat catccagtcg tccattggca cgttcataac gaccaaagat agtcgccata
    24781 tccataagtg tctgcggctt ttcagaacga ccaagttcat cacgcatggc gatatatttg
    24841 tcataagtcg cctgcaattg tttttggaat ttagaagctg aaaagtcgat gtttttcaac
    24901 tcagtttcaa aggttgtgtg acggcagtgg tctgaccagt aagtatccaa aactttcaac
    24961 tcagtttcag ttggcacacg cccgattgat ttgaaataat cttggatgaa gagaagatca
    25021 tcgacctcca tagccaagcc ctgctctgcc ttataggccg caaaatcgtc agccttataa
    25081 gtttcaaaga aatcaagact agggattgtc ttatcagaca cagagaaagc ctgctcttca
    25141 agcggcaagg taatgtcctt gaaacgtgaa tcaacaggat tcaaaagata gttcttaacg
    25201 gcttcaagct ctgcttctgc aatatcctta ttgaccaagt aaagctgggc tgtattgacc
    25261 ttaacctgac tgtcacttcc aagcaatagc aaagcttctt gcgaactagc agcacgttgg
    25321 tcaaattgac caggaagcgc ctcaatggca aagaaggcaa ccttatcaag ctccgcagtg
    25381 atttcagttt ccgtcaaaag acagtctgtc acctgctcag agaaaatatg cttctcagca
    25441 cgcgccagca aatcctcagc caaattgaag acatcataga cctgcacaat acgcaaatcc
    25501 ttcaaagaag tcagttgtag attatgcgtc aactctttca caagactagc cgatttaatg
    25561 tcaaagtcag cctttttctc aacaaaaata cgtttattca tataagatac caagtccgac
    25621 aagggagcga ctgaaaaata ggaggatatc tgaaattgag atttcagtaa gcccaatctt
    25681 ttttctgagt ttcccgaacg gattcaattc cttcctcttc taattacaat gctatcaact
    25741 tctcaagtac aacttgatag acatcggtca agctaccaag atctctacga aaaacatcct
    25801 tgtccatatg atttccttca gcatcccata gacggcagtt gtcaggtgag aactcatcag
    25861 ccaagatgat tttgccatcc ttgtcaaatc cgaactccaa cttgaagtca atcaagttaa
    25921 gcccaatctg agcaaaccaa tctttcaaga gttcattaat acggcgagtc tctcctttca
    25981 gataagcaat ttcttcatca ttaacaatac ccaaaaactt catatgctca tcattgataa
    26041 aaggatcatt cagatcatca tttttgtagt aaaattccac aattggagtt tcaagaacgc
    26101 gtccttcttc cactccaaaa cgttttgaaa atgagcctgc tgtcacattc cgtaatacca
    26161 cctcaagtgg aataatatca acttttttat tcaattgttc gttttttgaa atttgctcaa
    26221 tataatgtgt caccacacca gccatattta atttctcaaa aataagagat gaaatctgat
    26281 tatttagtgc tcccttgcca tcaatagtct ccttacgagc tccattcaac atagtagctt
    26341 ggtctttata gaccgttcta atcacatttt catcttttgt actataaata tctttcgctt
    26401 ttcctttata aataagctgg ttagtcactt gattattcgc ctttctctta ttttaagagt
    26461 tattctaaca cacaacatta tctaatacaa caaaaacttt atttaaaatc aaaaaaaaga
    26521 agaaacgttc gtattttctt cctttttatt attacttatg ttcaatgata taatctaata
    26581 gttgttggac gcttgacatg tgttcgacag cctcatctgg aatctctaga tgaaacacat
    26641 cttccaaatt gataataaac tctgttaaag cgatagagtc aattgctaaa tcatcatgta
    26701 acgatgactc tggtgtgata tctaactgtg ggtcaccaag ttgctgcctt aataattcca
    26761 gcatcttttc aaaaacttca tctcttgaca tgagttcact cctattcatc tgtaaaggca
    26821 tcaactaatt gatcaactac ttgagtttcc aacattgtac gaacttgctt caaagtagaa
    26881 taaacagctt tagaatcact tgatccatgg cattttacta ttggagcttt taaaccaaaa
    26941 agaaccgcac ctcccgcgct tgaatagtcc atactatctt ttagctgata caggctatct
    27001 tttaatagaa gtgctcctaa tttagctttc acgccacctg atttaattga agattttaga
    27061 cttcccataa tattcatagc tgtaccttcc attgtcttta gaacagcatt tccagtaaaa
    27121 ccatcagtga caacaatatc tgctacacta gacatcaaat cacgtgcttc aatatttcca
    27181 ataaaattaa tagaaggctc tgctgctaac agttcatagg cttctttatg aagagaatcg
    27241 ccttttgttt cttccgttcc gttatttaag agacctacac gaggaacttc aataccacgg
    27301 acattttttg cataaaacga ccctaaaata gcatattgat gaagatgaga agctgtgttt
    27361 tctgcattag ctcctaaatc aagcatatca aaacccacac catctaacgt aggcatagtt
    27421 gacattaagc ctggtcgatc aacacctttt atacgtccta ctacaaacaa accagcagct
    27481 aataaagctc ctgtattccc tgcagaaata aaggcctgtg caacaccatc tttgactgcc
    27541 ttagctccta agaccatcga agactgtttt ttacgacgaa cagctttagc aggctcatca
    27601 tcagaattaa ttttttcttc agtatgaata atactaactc gctccgttac tgttaaatat
    27661 ttctcaattt ttttctgatc gccatagagt tgtacttcaa tatcactaaa atctgaaata
    27721 gcttgattaa caccctctac aatagctttg ggagcgtaat ctccacccat agcatctaca
    27781 gcaattttct tcatgaatga actcctcata aaaacttagt ggctatatta tagcatgttt
    27841 ctttattttt ttcacaagaa ggcaataata gctaatatct aacagatact ttcactcttt
    27901 ccaaagaaaa gtatgctaaa caacctacta atatcaaaga acatagtact cccccttcaa
    27961 aaccaaaatc cccaccactc caaatagcaa agctagaatt ttcagtaatt ctaaaaagag
    28021 aaacaggaga atacccccca ctaaccttat tacctaatac acatcccata gtaaaattcc
    28081 atgcagtatg aactcctgta acaaaaagaa tatttttggt catattaaaa agaatagaaa
    28141 aaataatacc aagtaaaaat aaattaaaaa gtgctattgc tgtgatacca tagtttctaa
    28201 agtggataaa ggaaaacaac atagaattgc ataaaacacc tgcaaaacta cctttaaatt
    28261 tggtaactgt tgtcataaca tatcctctaa ataaaatttc ctctgtcaag ctttggataa
    28321 agtatgcaag taataaaaag aaaaaaggtc caaaagaaaa ggaattgctg gaagaaattt
    28381 ccacaaaacc tgataaccag tggcaaatat aaataaaaaa catcagtaaa ctaccagtaa
    28441 taaatcctga gatcgttgtt aaaacattat tatctttagt aaatcccaag gaagtaaaat
    28501 cccttttttg aaaatagatt aaaaatagta aaatacttgc caaaatagga ataaatagta
    28561 aaagcgcaat gtataaattg aatttataat catcaggtag caattttact acaaggcgtc
    28621 tacttacaat gatgccgata acttgaatta gaactgcacc aataactggc acaaaccagt
    28681 tagcaaaaaa gagtttattg gtaatgtata atgttttttt catctcaaat atcctaaaaa
    28741 ttaattccat attagtttat cctaatatta aaaaaaagaa catgacacta atgtcatgtt
    28801 cctctttaca acttaatctg atttcataat acttccccaa ctagacaaat catcaataaa
    28861 ttttttactt ttaagatgaa tccctacata ttcatcgtaa atcatgtcca aaaattgacg
    28921 aattttaagt ttcatctcag gtttcacaga aattgtttgt aaatcatcaa attgaattga
    28981 ctgaaaacga ttgattaagt acagcatatt aggatctagg tggtttcttc tctcgtcttt
    29041 ataataatgg tttggacata ataatccaga atatttgtgt gaaaaatcaa aaggtaatcc
    29101 tgccctatga caaaaaacac aatcataaaa atttaaagca acaccgaacc tctctagtag
    29161 ttgtacttca aaaatattcg ttagaatctc ataatctaaa ccgtcctcaa tcaattctaa
    29221 cgttttcttt aggaaaataa ataattgagc atccgctaca ttatcactaa tagccacatc
    29281 agccaaagaa gtaatataac tcgcatatga caacttaaac aagtctgaat tggtttcttg
    29341 aaatgctaaa acttctttat aatcatcaat ataagaaaga ccattatcat taatttttaa
    29401 aataaaatga gcaatagtca aaggttggag cacagcattg aattttgact ttgaagcatg
    29461 ctttacaaaa aacattcttt tcccctcagt ctccgtaaag attttaacta atttatcgtc
    29521 ttcacgataa ttacgattat acaaaacgag accgtatgtt tgactaaccc tcatatgatt
    29581 ccataaaaac tttcaatcta gccattgctg tttcaataac ttccatacta gcagcataag
    29641 aaagacgtat atacccttca ccatatttac caaaagccac ccctggaata atagctacag
    29701 cttgttgata agcaaaatcc tgacaaaact tgaaagaatc ctgtggataa ctatcaggta
    29761 ttttagcaaa gatataaaaa gcgccgctcg gtttaattat tttgaaacca agtttagaca
    29821 ttttatcaat gatataatct ctacgtttaa tatatccttg acgcataggt aacgtatcat
    29881 ttttacccac agataaggct tctaccccag caaattgcga aatagtacta gcagctgtca
    29941 ccatgtattg atgactttta atgatttgag cagtagatgc ttcaggagca tacaccaatc
    30001 caactctcca accagtcata gcatgtgatt ttgataagcc attaattaag atagtttggt
    30061 taggtaaata ttctgcaatt gaaacatgtt gctgaccagt gtaagttaac tcagagtaaa
    30121 cttcatcact aataacaaaa atatcatatt ttttaaggac ttcagctaat gcagcaattt
    30181 cttgccttga ataggtaata cctgtaggat tagtaggata attaagtaaa acagccttta
    30241 atttttcacc ttgttggata atagccgttt ctaacatttc aggggttagt ctaaagtcat
    30301 tttctctagt atcaatttct acgatatcag ctcctacaag gttaactatt ggctcatagc
    30361 ctggataagc gggggcaggt aatagaacaa catcaccagc ttctaaaata gctataagag
    30421 atgcagacag agcttctgtg gcacctatcg taaccaagat ctcactatca gggttataag
    30481 taagattata tttatccttt gcaaaatctg cagcagcttg ccttagagct aataaaccgc
    30541 tcataccagt ataatacgat tgattcgcat caatagctga tttagctgct tctttaacat
    30601 gatcaggagt ggtaaaatct ggttctccaa gagttaattt taccattcca ggtatatctg
    30661 aaatagattg atcaaactga cgaattaagg agacctctat tctatctaag tacttattaa
    30721 atcgtttctc taatgtcatc ataacctcct gataagaaat aatatattta attatacaag
    30781 aaaaataatt tgtgtccagt tttccgtcat taaatgataa aaaacctgtt aataacaggt
    30841 tttttatcat tttagtttat tcatttcaaa taatggtgat aaaggacgtt tctcgtgaat
    30901 acggataata gcttcaccaa tcaattccgc aattgaaatt tgttcaatct tatcaatcaa
    30961 acgttcttca gggaggtaaa tcgtatctaa cacaattaat ttttcaatag ctgaattttg
    31021 aatattatcc aaagccggcc ctgataaaac agggtgagta catgaagcat aaacagctgt
    31081 tgcccctgct tcagctaaag cgtctgcagc atggcaaata gtacctgcag tatcaatcat
    31141 atcatcaatt aaaatacatt ttttaccttt gatattacca ataatattca ttacttcaga
    31201 tgtattcatt ttagtaacac tacggcgctt atcaatgata gcaatcggtg tctttaaaca
    31261 ctgtgccagc ttacgtgcac gtgttacgcc accatgatca ggactaacga caacaacatc
    31321 atcaccaact aatccctgac gatcaaagta atctgcaata aggggagccc ccataaggtg
    31381 atcaacggga atatcaaaga atccctgaat ttgagctgca tgtaaatcaa ctgttaaaag
    31441 acgatcaaca ccagcaactt ctagcatgtt cgcaacgagt ttcgaagtaa taggttcacg
    31501 agaacgtgcc ttgcgatctt gacgagcata cccatagtaa ggcattacca cactaacaga
    31561 ctccgcacta gcacgcttta aagcatcaac cataattaaa atttccatca aattatcatt
    31621 aactggtgag cttgttgatt gtaaaataaa aacatgatgg ccacgaatag actcttcaat
    31681 attaacttga atctcaccat cagaaaattg gcgaactgtc gattgaccta gcggaattcc
    31741 aatcgtctga gacacttttt tcgctagttc tttgtttgat gacagtgcaa acaattttaa
    31801 attagaataa gacatgattt cctccaaaac tttagtccta taccatttta acgcttttta
    31861 cacaattttt caatccaaaa taaaccatac ctaattaata aacaaaaagg agaccacgaa
    31921 agatctcctt agtattcatt aatttgggta aatatagtaa acagagccag aagcactagg
    31981 gttaaatgaa ccacggtaat tgccaatgct catatttcca gcatagtttg attccataac
    32041 ttggatagat gaattgttag ccactgacgt tactactgca acgtgcccgt aaccaccacc
    32101 atcatatggc caaacagcta ctgcaccaac acgaggggta gttcctacag aataaccagc
    32161 tgcacgtgca ctagcacccc actgattagc atttccccaa tagttaccaa cccaagaagc
    32221 cattgactta gctccccaag tacattgtcc cattgggtat gtgttacttg aatcataact
    32281 tctgacaaca ggttttgata ccgctctagg tgttgaagaa acaactgctt taggtgcttc
    32341 aactgcttta gttactacag aaggttgagt aacttttggc tcattagtag ctgtagctgt
    32401 agttttagca acagtagttg ccgaagaagt agccgtaaga ttagaaggct ttatttcagt
    32461 tctcggttgt gtttgaactg tttcagtagg agctgtggca gactcaactt gtgctgctgc
    32521 ctgtgctttt gctacagact cagcttgtgc tttcgcttca gctactgctt gtgcttcagc
    32581 tgccttacgc gcagcttcct cagcttgtgc ttttgcctga attaaagatg ctttttcatt
    32641 ttggactgta gtcaattgag cagataactc taattgagct gcttctaatt gtgctctttg
    32701 tgttgctaag gctgctttat tattttcgat agcttgcttg ttagctgcta cagtatttat
    32761 agcattttga ttttcaattt gtttagcttc taaggctgct ttgtcagcct cttgttgtgc
    32821 taacatcttt tcattagctg aaacaacctc acgaatagca acaacacgat taacagcatc
    32881 agatactgat ttcgaattta aaatagtatt aatatagtta gtcaagttac ccttttgagc
    32941 actacgaact tgctttttca aagattcgtt acgtgcaaca attttattag aaagagtttg
    33001 aatctcttga cctaactgct gagatactgc ctcaagttga gcattttgcg cttctaattc
    33061 tgattgttga gactctaaag caccaacttg tcctttaata gcagtcacct ggttttgtgc
    33121 tgctgcttgt tgccctgata aagtattgat tactgaatca gtagcagcaa ttttcgagtc
    33181 aaagtcatca gcgtttactg ttacggcagc agttccaaga gttactccac tcacaaggac
    33241 tgctgataat attctttttt tcatttttat taaactactc ctttacgata agacattaaa
    33301 tattttacca aaaaattcac gaaattatat tacgtcattg ttacatttat atttgaaatc
    33361 aactatttct aaatgaacca taatcaaatc tagaaaacga taaccttctt ctattcactc
    33421 tatcaatata attactccat agtgaaacta aaagagaaat aaaaaaagag tataattact
    33481 cttaaaatta taatatttac ggagaataag ggattcgaac ccttgcgcca gttacccgac
    33541 ctaacgattt agcaaaccgt cctcttcagc ctcttgagta attctccaaa ttaatattaa
    33601 tgggcacgag tggactcgaa ccaccgacct cacgcttatc aggcgtgcgc tctaaccacc
    33661 tgagctacgc gcccaagcaa atgcttggtt ttacttttat gtaaagtaag cgggtgacga
    33721 gaatcgaact cgcgacaaca gcttggaagg ctgtagtttt accactaaac tacacccgct
    33781 aaaaacttat at
//
LOCUS       DATRUK010000017        32731 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.017, whole genome shotgun sequence.
ACCESSION   DATRUK010000017 DATRUK010000000
VERSION     DATRUK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32731
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.017"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..792
                     /locus_tag="VBE34_000753"
     CDS             <1..792
                     /locus_tag="VBE34_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEO7263486.1"
                     /translation="FAYREQLQNKNIEEVMELVDSDLNHYFERVDTQVYLFNDDTLIG
                     RYMVYLGKNYHLIEDGYNCFQAKLFLGGSVVKRVIKTYLFKKYVPYGFSKYCLSIEVN
                     SLVGLPHDIRSKKYKELPRKKLFDSLNKEQKSLIFKIFKTKPLTITPKSVLLLTQPLA
                     QDKCYKTPTERFQSIQEQYDYFDDIVQEYRTLGYNVYLKVHPRDVVDYSKLPVELLPS
                     NVPMEIIELMSTGRFECGITHSSTALDFLTCVDKKITLVDLKDIK"
     gene            789..2189
                     /locus_tag="VBE34_000754"
     CDS             789..2189
                     /locus_tag="VBE34_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO7263487.1"
                     /translation="MNTKKLLQSGFIYTLGNLLVQGLAFITLPIYTRVISTEVYGQYS
                     LYVAWMNIIMLFIGLQTSGSLSSARVKYGEEFKSYSGSAFSVGNIWFLIILLIAFLFR
                     SFLAPLVGFSESIFLLMVCQSYASYVVTFFGQYFIQQQRSLANLILSLANAVSSVALS
                     LFLIFHWSDDFLSRVFGAFVPTIITGIVAFAYIYYHSKSFYNPKYFRFIVTVSVPLIF
                     HLLGHQLLGQLDRIMLARLYNTKEVAMYSFGYSLGMIIQIVLNSINMAWIPWFFDARK
                     EKLLQLSTYISRYLYLGVFLTLGYLTVFPELAQIMGGDKYSSSVQFISLIIVSYFLVF
                     LYTFPVNIQFFYANTTWIPIGTLLAAGVNWLLNLVLIPHYAAYGAAMATIISYLALLI
                     FHHIVSKVKYHYSDVSVRQYIILSGIVFSYAMLMNMFLDTIVIRWSLGIIILIVYSIV
                     FQKVILDLLSKKRRRR"
     gene            2189..3214
                     /gene="neuB"
                     /locus_tag="VBE34_000755"
     CDS             2189..3214
                     /gene="neuB"
                     /locus_tag="VBE34_000755"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEO7263488.1"
                     /translation="MVYIIAEIGCNHNGDINLAKKMVDVAVSCGVDAVKFQTFKAEKL
                     ISKFAPKAEYQKATTGTADSQLEMTKRLELSFEEYLEMRDYAISKGVETFSTPFDEES
                     LEFLISTDMPIYKIPSGEITNLPYLEKIGKQQKKVILSTGMAVMEEIHQAVNILRQNG
                     TTDISILHCTTEYPTPYPSLNLNVIHTLKDEFKDLTIGYSDHSIGSEVPIAAAAMGAE
                     VIEKHFTLDTNMEGPDHKASATPDILAALVKGVRIVEQALGRFEKIPDPVEEKNKIVA
                     RKSVVALKPIKKGDIYSIENITVKRPGNGISPMNWYDILGQEAQDDFEEDEVIRDSRF
                     ENQLPEL"
     gene            3291..4445
                     /gene="neuC"
                     /locus_tag="VBE34_000756"
     CDS             3291..4445
                     /gene="neuC"
                     /locus_tag="VBE34_000756"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEO7263489.1"
                     /translation="MKKICLVTGSRAEYGIMKPLIQRLSKDKEVNLQIIATAMHLEEK
                     YGYTYRQIEEDGFDIAYKVPLHLYDTDRRTVSTAMAHLQLGLTKIFDKEDYDLVIILG
                     DRYEMLPVVNVALIYNVPVCHLHGGETSLGNFDEYIRHAITKMSHLHLVSTEDFRQRV
                     IQMGEQPQFVINTGALGVENALSIPSLTKEAIEKQLGIVLEESYFVVLYHPVTFEQGK
                     SAGEQMKAVLSALSKFGVQCLFIGSNSDTGSDDIAKAINTYLINHENSYCFASLSTQL
                     YHSLIRHSLGLIGNSSSGLIEVPSLMKPTLNIGDRQKGRLHGESVVSVPVETPSVLEG
                     LSKLNEVTNFDNPYYKENASSIAYEAIKLYLKDEPSISQPFYDLKENNLK"
     gene            4442..5071
                     /locus_tag="VBE34_000757"
     CDS             4442..5071
                     /locus_tag="VBE34_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO7263490.1"
                     /translation="MKKKLAFLGAGSHADAIAPVIDPLVYDFVGFFDDKDITEHDGYP
                     VLGKLYDVLPYLEDGSIDAVFITIGDNAKRKELFEYVAKDYYDFIINIISPNALVLTP
                     DSICGRGIFIGFGAFIGSKVKLFDNNVVNTGALIEHHTVVESHCNIAPNATINGLCYI
                     REEVYVGSASVIIQTLDISSCTTIGAGAVVVKDIIEPGTYVGVPAKKIK"
     gene            5082..6323
                     /gene="neuA"
                     /locus_tag="VBE34_000758"
     CDS             5082..6323
                     /gene="neuA"
                     /locus_tag="VBE34_000758"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEO7263491.1"
                     /translation="MKPICIIPARSGSKGLPDKNMLFLAGKPMIFHTIDAAIESGMFD
                     KKDIFVSTDSELYREICLERGISVVMRKPELSTDQATSYDMLKDFLSDYEDNQEFVLL
                     QVTSPLRKSWHIKEAMEYYSSHDVDNVVSFSEVEKHPGLFTTLSDKGYAIDMVGADKG
                     YRRQDLQPLYYPNGAIFISNKETYLREKSFFTSRTYAYQMAKEFSLDVDTRDDFIHVI
                     GHLFFDYAIREKENKVFYKEGYSRLFNREASKIILGDSKTISISLENYHNYSQGGVTL
                     ATMLENLPNFLTANVTEAFVSIGVNDLITGHSVEEIFSNFQKLYSLLAENKIKMRLTT
                     IAYTLFRETVNNADIEKINQWLTEFCYQNQIPLLDINRFLSKDGNLNYHLTSDGLHFT
                     QEANDLLQSQYQLFVDEVKTL"
     gene            6437..6922
                     /locus_tag="VBE34_000759"
     CDS             6437..6922
                     /locus_tag="VBE34_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263492.1"
                     /translation="MRETETHDHQALIQKLLVSIHYLTLFRDEIILVEKTPSLLGKHF
                     SIAIVQNELGEILSKIEALSKQKKLIRSIYWYDESSFKVMNKALAIVEEWIKGLDNLL
                     EFCQSQTVFQAILGDERAHVFGILIDVYTSLNIINTSLKEENSRPVSLSDLALGLKDE
                     D"
     gene            7021..7674
                     /locus_tag="VBE34_000760"
     CDS             7021..7674
                     /locus_tag="VBE34_000760"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEO7263493.1"
                     /translation="MKHSSWHDLIKRELPNHYYNKINTFMDAVYESGIVYPPRDKVFN
                     AIQITPLENVKVVIIGQDPYHGPQQAQGLSFSVPDNLPAPPSLQNILKELAEDIGSRS
                     HHDLTSWAQQGVLLLNACLTVPEHQANGHAGLIWEPFTDAVIKVVNQKETPVVFILWG
                     GYARKKKSLIDNPIHHIIESPHPSPLSAYRGFFGSRPFSRTNHFLEEEGINEIDWLN"
     gene            complement(7740..8378)
                     /gene="plsY"
                     /locus_tag="VBE34_000761"
     CDS             complement(7740..8378)
                     /gene="plsY"
                     /locus_tag="VBE34_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEO7263494.1"
                     /translation="MNIIIMIIIAYLLGSIQTGLWIGKYFYQVNLRQHGSGNTGTTNT
                     FRILGVKAGIVTLTIDILKGTLATLIPIILGVTTVSPFFIGFFAIIGHTFPIFAQFKG
                     GKAVATSAGVLLGFAPSFFLYLLVIFLLTLYLFSMISLSSITVAVVGILSVLIFPLVG
                     FILTDYDWIFTTVVILMALTIIIRHQDNIKRIRKRQENLVPFGLNLSKQKNK"
     gene            8492..10441
                     /gene="parE"
                     /locus_tag="VBE34_000762"
     CDS             8492..10441
                     /gene="parE"
                     /locus_tag="VBE34_000762"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEO7263495.1"
                     /translation="MTKQDITVTNYGDDAIQVLEGLDAVRKRPGMYIGSTDGTGLHHL
                     VWEIVDNAVDEALSGFGNRIDVIINKDGSITVTDHGRGMPTGMHAMGKPTVEVIFTVL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEIIRDGAIYRQRFENGGKPVTTLK
                     KIGTAPKSKSGTSVSFMPDQSVFSTIDFKFNTIAERLKESAFLLKNVTLTLTDNRSEE
                     AEHLEFHYENGVQDFVEYLNEDKETLTPIMFFEGEEQEFHIEVALQYNDGFSDNILSF
                     VNNVRTKDGGTHETGLKSAITKSMNDYARKTGLLKEKDKNLEGSDYREGLSAILSILV
                     PEEHLQFEGQTKDKLGSPLARPIVDGIVSEKLTYFLMENGDLASNLIRKAIKARDARE
                     AARKARDESRNGKKSKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTAKAKMADIIKNEEINTMIHTIGAGVGPDFNLDDINYDKVIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEEGHVYIALPPLYKMSKGKGKKEIVEYAWTDIEL
                     EELRQKFGKGSLLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLM
                     GDKVPPRRQWIEDNVKFTLEENTVF"
     gene            10575..13034
                     /gene="parC"
                     /locus_tag="VBE34_000763"
     CDS             10575..13034
                     /gene="parC"
                     /locus_tag="VBE34_000763"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEO7263496.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGFRKSAKSVGNVMGNFHPHGDSSIYDAMVRMSQDWKNRETLIEMH
                     GNNGSMDGDPAAAMRYTEARLSEIADYLLQDIDKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGATGISAGYATDIPPHNLAEVIDAVVYMIDHPKAKLDKLMEFLPGPDFPTGAI
                     IQGKDEIRKAYETGKGRVAVRSRTAIETLKGGKKQIIVTEIPYEVNKSVLVKRIDDVR
                     VNNKVPGIAEVRDESDRDGLRIAIELKKEADETIVLNYLFKYTDLQVNYNFNMVAIDD
                     YTPKQVGLSRILTSYIAHRREIIIARSKFDKEKAEKRLHIVEGLIRVLSILDEVIALI
                     RASENKADAKENLKVSYEFSEAQAEAIVTLQLYRLTNTDIVTLREEEEELRQQITMLK
                     AIISDERTMYNVMKRELREVKKKFANTRRSELQELAETIEIDTASLIIEEDTYVSVTR
                     GGYVKRTSPRSFNASTVDELGKREDDELIFVSNAKTTQHLLMFTNLGNLAYRPVHELA
                     DIRWKDVGEHLSQNLVNFASNEEIIYAELVDDFTKETYFAVTSLGQIKRFERQEISPW
                     RTYKSKTAKYAKLKNVDDYVVTVAPIQLEDVILVTYNGYVLRFSINDVPVVGSKAAGV
                     KAMNLKDSDHIVSAFIANTTSLYLLTHRGSLKRMAIDVIPTTSRANRGLQVLRELKSK
                     PHRVFKAGPVYLEDSSFEFDLFSSVSNHEGDTFVLEIMSKTGKVYDVDLSQWSFSERT
                     SNGSFVSDKISDEEVFSVKIK"
     gene            13148..14170
                     /locus_tag="VBE34_000764"
     CDS             13148..14170
                     /locus_tag="VBE34_000764"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEO7263497.1"
                     /translation="MTVNLDWDNLGFAYRKLPFRYISHFKDGKWDDGKLTDDATLHIS
                     ESSPALHYGQQAFEGLKAYRTKDGSIQLFRPDQNAERLQRTADRLLMPHVPTDKFIAA
                     VKSVVRANEEFVPPYGTGATLYIRPLLIGVGDIIGVKPAEEYIFTVFAMPVGSYFKGG
                     LTPTNFIVSKEYDRAAPNGTGAAKVGGNYAASLLPGKYAHEKQFSDVIYLDPATHTKI
                     EEVGAANFFGITKDNQFITPLSPSILPSITKYSLLYLAKERFGMEAIEGDVFVDELDK
                     FTEAGACGTAAVISPIGGIQNGDDFHVFYSETEVGPATRKLYDELVGIQFGDVEAPEG
                     WIYKVD"
     gene            14253..14483
                     /locus_tag="VBE34_000765"
     CDS             14253..14483
                     /locus_tag="VBE34_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001870505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEO7263498.1"
                     /translation="MSKKDKKIEIQILDAQVTVNGTKVDGYQLVIGKKIVGQIAELDS
                     KFAVIKGEQVSGFYKNLDQAMGNIIEEYNLNR"
     gene            14544..14624
                     /locus_tag="VBE34_000766"
     tRNA            14544..14624
                     /locus_tag="VBE34_000766"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14578..14580,aa:Tyr,seq:gta)
     gene            14630..14701
                     /locus_tag="VBE34_000767"
     tRNA            14630..14701
                     /locus_tag="VBE34_000767"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14662..14664,aa:Gln,seq:ttg)
     gene            14827..16029
                     /gene="rpsA"
                     /locus_tag="VBE34_000768"
     CDS             14827..16029
                     /gene="rpsA"
                     /locus_tag="VBE34_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEO7263499.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIDGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVVEESAAAARKEVFSNIEVGSVVTGKVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVTVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AAGDVIEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSAGQEVTVKVL
                     EVNSDAERVSLSMKALEERPAQAEGEKEEKRQSRPRRPRRQEKRDYELPETQTGFSMA
                     DLFGDIEL"
     gene            16102..16173
                     /locus_tag="VBE34_000769"
     tRNA            16102..16173
                     /locus_tag="VBE34_000769"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16135..16137,aa:Arg,seq:ccg)
     gene            complement(16442..17065)
                     /locus_tag="VBE34_000770"
     CDS             complement(16442..17065)
                     /locus_tag="VBE34_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEO7263500.1"
                     /translation="MIPLKKITVGTIVCLSFLGLTACSSSNTQQTSTSKSNVSQHKNI
                     KADHEELRLKFNKVKLGVKANNFKGGTSLAELKQLFGGEPNEKFDTPAGNVTLKGYRW
                     NVDDISITIQLLNDSSIVRSISNFKFIRDANITTKDYNSLKNGMSYNKVKELLGEPDD
                     ISQAVSSDKEELQAAWISGIQSSDSDPGINLTFENDKLTNKQQHGLK"
     gene            complement(17134..17829)
                     /locus_tag="VBE34_000771"
     CDS             complement(17134..17829)
                     /locus_tag="VBE34_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEO7263501.1"
                     /translation="MATLTNNPIFGIMLTVWAYYIGIRIFRKYPSPATTPLLLATILL
                     IAFLKLTHISYKDYYNGGSFLTMLITPSTVVLAIPLYRTFHLMKHHIKSISISIILAS
                     VINTVFTAIVAKFFGMKYFLAISLFPKSVTTAMAVGITSKAGGLATITLVVVVITGIL
                     TSVLGPIFLKLLKIEDPVAIGLALGGTGHAIGTGQALKYGQVQGAMAGLAIGITGICY
                     VIVSPLVAGLILK"
     gene            complement(17810..18202)
                     /locus_tag="VBE34_000772"
     CDS             complement(17810..18202)
                     /locus_tag="VBE34_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO7263502.1"
                     /translation="MKLYVQLMVILTFSFAGEVLSTIFNLPIPGSIIGLILLFLALKY
                     KIIRLRHIDTVGNFLLANMTILFLPPAVGLMEHFQDIKPYLFGIAIIILGALFLNILT
                     IGLVSQWIKKRYEGDYPEIGGKNGNFNE"
     gene            complement(18361..19596)
                     /locus_tag="VBE34_000773"
     CDS             complement(18361..19596)
                     /locus_tag="VBE34_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO7263503.1"
                     /translation="MATSTENLQLAKRGPIISIIAYITLAVAKLAAGYWFDATSLVAD
                     GFNNLSDILGNVALLIGLHLASQPADSNHRFGHWKIEDLASLITSFIMFVVGIQVFIQ
                     TVTKIINNTDTNIDPLGAIVGAISALVMLGVCFYNKQLSQRVKSSALVAASKDNLSDA
                     VTSIGTSIAIIAASLNFPIIDRLAAIIITYFILKTAYDIFIESAFSLSDGFDDYQLKQ
                     YEKAILTIPKISAVKSQRGRTYGSNIYLDIVLEMNPDLSVFESHAITEKVEKLLSDKF
                     SVYDIDIHVEPASIPEDEIFDNVYQKLYKNEKIILAKIPGYETFISPDFYMINEKGNI
                     ITSDMLTNATNHSLASNFKYFNVKSISQKTKLVSYELEGKRHTSIWRRNEKWFLIYHQ
                     ITAKSSPYKTRRYQITSLD"
     gene            complement(19660..21006)
                     /locus_tag="VBE34_000774"
     CDS             complement(19660..21006)
                     /locus_tag="VBE34_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEO7263504.1"
                     /translation="MLTLFTHINSFVWGPPLLALLVGTGIYLSFRLGFVQLRQLSRAF
                     KLIFREDNGQGDISSYAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGLLAIKYRTKDTNGEISGGPMYYIVNGMGQKWKPLAVFFSAAGILVALLGIG
                     TFTQVNAIASSLEHTFKISTRFTSLILAVIVLFIIFGGIKSISKVSEKIVPFMAISYI
                     LATLIIIAVNYNKIPHTFQLIFSGAFSGTAAIGGFSGAIVKEAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTKEPVEQGLISMTGTFIDTIVICTLTGIAILVTGKWLEFDLQGAPL
                     TQASFNTVFGSLGSFALTFCLVLFAFTTILGWSYYGERCFEYLLGTKFINAYRIIFVI
                     MVGLGGFLQLDLIWVIADIVNGLMALPNLIALLALSPIIVKETQKYFSETKNITKP"
     regulatory      complement(21163..21295)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            21603..22928
                     /locus_tag="VBE34_000775"
     CDS             21603..22928
                     /locus_tag="VBE34_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEO7263505.1"
                     /translation="MTLNAKGESNQILVDKEVHSTNLLLDIDEKPELFQGLLLSFQHV
                     FAMFGATILVPLILGMPVSVALFASGCGTLIYQVATKFKVPVYLGSSFAYITAMALAM
                     KQMHGDISAAQTGILFVGLIYVVVATVIKFVGNSWVDKILPPIIIGPMIIVIGLGLAN
                     SAVTNAGFVAKGDWRKMLVAVVTFLIAAFINTKGKGFIKIIPFLFAIIGGYILSIILG
                     LVDLSPVEKAAWFELPKFYLPFKTGLFHSYKLYFGPEMLAILPISIVTIAENIGDHTV
                     LGQICGRNFLKKPGLNRLLIGDGLATAFSALIGGPAETTYGENTGVIGMTRIASVTVI
                     RNAAFIAIAFSFFGKFTALISTIPSAVLGGMAILLYGVIASNGLKVLIENRVNFAEVR
                     NLIIASSMLVLGLGGAVLDLGALTLSGTALSAIVGIILNLILPKESMKS"
     gene            23061..23447
                     /locus_tag="VBE34_000776"
     CDS             23061..23447
                     /locus_tag="VBE34_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEO7263506.1"
                     /translation="MKFNLEQVKVFENYEIENWEEGQVTLTTKVVDSSLNYYGNAHGG
                     YLFTLCDQVSGLVAISTGYEAVTLQSNINYLRAGRLDDLLTVIGTCVHNGRTTKVVDV
                     HIKNQKDEMVTTAIFTMFVTGERKEK"
     gene            23553..25832
                     /gene="pcrA"
                     /locus_tag="VBE34_000777"
     CDS             23553..25832
                     /gene="pcrA"
                     /locus_tag="VBE34_000777"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEO7263507.1"
                     /translation="MNPLIIGMNDKQAEAVQTTDGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKYVNPWNILAITFTNKAAREMRERAIALNPATQDTLIATFHSMCVRILRREADYI
                     GYNRNFTIVDPGEQRTLMKRIIKQLNLDTKKWNERSILGTISNAKNDLLDEIAYEKQA
                     GDMYTQVIAKCYKAYQEELRRSEAMDFDDLIMMTLRLFDQNKDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPQAKVVL
                     LEENYRSTKKILQAANNVINHNKNRRPKKLWTQNDEGEQIVYHRANNEQEEAVFVAST
                     IDNIVREQGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVIA
                     YLNILANTSDNISFERIVNEPKRGVGPGTLEKIRSFAYEQNMSLLDSSSNVMISPLKG
                     KAAQAVWDLANLILTLRSKLDSLTVTEITENLLDKTGYLEALQVQNTLESQARIENIE
                     EFLSVTKNFDDNPEITVEGETGLDRLSRFLNDLALIADTDDSATETAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRAIEDADELEEERRLAYVGITRAEQILFLTNANTRTLF
                     GKTSYNRPTRFIREIDDELIQHQGLARPVNSSFGVKYSKEQPTQFGQGMSLQQALQAR
                     KSNSQPQVTAQLQALNANNSHETSWEIGDVATHKKWGDGTVLEVSGSGKTQELKINFP
                     GIGLKKLLASVAPISKKEN"
     gene            26244..26489
                     /locus_tag="VBE34_000778"
     CDS             26244..26489
                     /locus_tag="VBE34_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO7263508.1"
                     /translation="MGLIWSLIVGAIIGAIAGAITNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLADMALIPSIVGAIIVIIVTSFVLGKKS"
     gene            26537..26785
                     /locus_tag="VBE34_000779"
     CDS             26537..26785
                     /locus_tag="VBE34_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO7263509.1"
                     /translation="MSLIWSLIVGAIIGAIAGAVTNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLAGMALIPSIVGAIIVVIVTSFVLGKMNN"
     gene            26800..27381
                     /gene="amaP"
                     /locus_tag="VBE34_000780"
     CDS             26800..27381
                     /gene="amaP"
                     /locus_tag="VBE34_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEO7263510.1"
                     /translation="MSKGLKSLYTLLGLISLTLLGFVAVISKQHIYLPSFNWLDWDFN
                     LPSPIDVGMYHYFFWGALVLFVIVLLAILVVLFYPRRYTEYKLADKTGKLMLKKSAIE
                     GFVKTEVLKTGLMKSPSVTAHLYKKKVKVDVKGLLTSRTNVPEQLEHIQSDVELGLKE
                     FFGLEKKMNTRVFVKQVEEENVGNAKTNKSRVE"
     gene            27391..27585
                     /locus_tag="VBE34_000781"
     CDS             27391..27585
                     /locus_tag="VBE34_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEO7263511.1"
                     /translation="MSEFVRKYRYPLGGAVIGLVLAAMIVTIGFFKTILALVIIVLGA
                     YAGLYVQRTGMLDQFFNKRK"
     gene            27641..28183
                     /locus_tag="VBE34_000782"
     CDS             27641..28183
                     /locus_tag="VBE34_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7263512.1"
                     /translation="MTETYIKNTTNNSGTTAVRGELTFEDKVIEKIVGIAIEHVDGLL
                     AVNGGFFSNLKNSVVNSDSVTDGVNVEVGKKQVAVDLDIVAEYQKHVPTIFADIKKVV
                     EAEVKRMTDLEVVEVNVNVVDIKTRAQHEEDSVTLQDRVTSAAQATGEFASNQVSNVK
                     SAVGNGVDKVEDMKSEPRVQ"
     gene            28230..28427
                     /locus_tag="VBE34_000783"
     CDS             28230..28427
                     /locus_tag="VBE34_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO7263513.1"
                     /translation="MSEEKFDAKVDKVSGSVKESVGKLTGDKEVESEGKVDKLKGHAK
                     EKLADIKDTIKGASESFKKKD"
     gene            28449..29000
                     /locus_tag="VBE34_000784"
     CDS             28449..29000
                     /locus_tag="VBE34_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7263514.1"
                     /translation="MENQKQDIKTSVTYEEKVIAKIVGHALESVDGLLAVSGGFFSNL
                     KNSVVNSDSVTDGVNVEVGTKEVAVDLDIVVEYGKDIPAIVESIKAIVSQNVEVMTHL
                     KVVELNANVVDVKTKAEHEADSVTVQDRVSDAAQATGNFASEQAGKAKAAISSGAEKT
                     KEAVSNGTEAAKEKISEARTSES"
     gene            29164..29805
                     /locus_tag="VBE34_000785"
     CDS             29164..29805
                     /locus_tag="VBE34_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7263515.1"
                     /translation="MVSEQSEIVTSKYQKIAVAVAQRIANGDYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLQILTLKHGSGAIILSKEKAIAFLNQYETSHSVAILKGKIR
                     DNIKAQQQEMEELATLVDDFLLQTRAVSKQYPLAPYEIIVSEDSEHLGKSIGELNVWH
                     QTGATIVAIEHEGKFIVSPGPFSVIEQGDHIFFVGDEDVYARMKTYFNLRMGL"
     gene            29810..30574
                     /locus_tag="VBE34_000786"
     CDS             29810..30574
                     /locus_tag="VBE34_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO7263516.1"
                     /translation="MDFVFDIDGTLCFDGMSLSKEIQGILERAQIDYGHRVTFATARS
                     YRDTIGILGDKLSLSKIIGLNGATLHENGHLVDSYYLQSDFFSTIISYCHRYQIPYFV
                     DEVFNYATYQASKIPFIAYVDPQKRGELLEVSKIEKPIKMVLYFGDQLRRADQMLAKL
                     NRFGLSSHFFHEFEKCLYINPIAVDKGKATKKLFGNRFIAFGNDKNDISMFDAAHYSV
                     QVGDFDELTPYANLRVSRESVHEGITMLFEKYKGIT"
     gene            30574..31233
                     /locus_tag="VBE34_000787"
     CDS             30574..31233
                     /locus_tag="VBE34_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEO7263517.1"
                     /translation="MDLFLSLERKFKAASDKEVSKQQEAYLRHHFKCYGIKSPERRML
                     YKELIKAAKRQAKIDWQLLDKCWQSDYREYHHFVLDYLLAMSQFLTYNDCSRLEFYAR
                     HQQWWDSIDVLTKIFGNLSLKDDKVMNLLSEWSLDQDFWMRRLAIEHQLGFKEKTNTD
                     ILSLFILRNTGSQEFFINKAIGWALRDYSKYNKVWVKDFISNHYDELSTLSIREGSKY
                     L"
     gene            31306..31800
                     /gene="tpx"
                     /locus_tag="VBE34_000788"
     CDS             31306..31800
                     /gene="tpx"
                     /locus_tag="VBE34_000788"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEO7263518.1"
                     /translation="MTTFLGNPVTFTGKQLQVGDIAKDFLLIATDLSQKSLKDFEGKK
                     KVISVVPSIDTGICSKQTRTFNEELSELDNTVVITVSMDLPFAQKRWCSAKGLDNVIL
                     LSDFYDHSFGQEYALLMNEWHLLTRAVLILDEHNKVTYTEYVDNVNSDVDYEAAINAA
                     KILP"
     gene            complement(31876..32409)
                     /locus_tag="VBE34_000789"
     CDS             complement(31876..32409)
                     /locus_tag="VBE34_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEO7263519.1"
                     /translation="MKEKKNLGIKLIKSLYGINGPFDEYREQKINYLGNLLFATTSLY
                     HILSSIIAWFTVSKYPTETACTLLALTFFLYFCIFLIVIPSYLNSHDLITPLEVSKND
                     YPVAIKKNKQKFIKIGIFYTVIQWLILGLTTNNNTPLIVSLFSLKVILSSIMSGVAYN
                     GVMYLIFKNNVDIEKDE"
     gene            complement(32509..32706)
                     /locus_tag="VBE34_000790"
     CDS             complement(32509..32706)
                     /locus_tag="VBE34_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7263520.1"
                     /translation="MNRLREYRIKASISQLALAKSVGVARQTINLIENNKYNPSLDLC
                     IRLAECLQTDLNTLFWDSRHL"
BASE COUNT        10800 a         5006 c         6369 g        10556 t
ORIGIN      
        1 tttgcttatc gggagcagtt acaaaataaa aatattgaag aagtgatgga gcttgtagat
       61 tcagacttaa atcactactt tgagagagta gatacacaag tctatctatt taatgatgat
      121 actttaatag gtcgctatat ggtgtattta ggtaaaaatt atcatctaat tgaagatggt
      181 tataactgtt ttcaagccaa gttattcttg ggtggttctg tcgtaaaacg agtaattaaa
      241 acttacttat ttaaaaagta tgttccgtat ggtttttcaa aatattgtct ctcaattgag
      301 gttaattctt tagtggggtt acctcatgac ataaggagta agaaatataa agaactaccg
      361 agaaaaaaat tatttgatag tcttaacaaa gaacaaaaat cactgatttt caaaatattt
      421 aaaacaaaac cattaactat aactccaaag tcagtattat tgttgacaca gccacttgca
      481 caagataaat gttataaaac acctacagag aggtttcaaa gtattcaaga gcaatacgat
      541 tattttgacg atattgtcca ggaatataga acgttagggt acaatgttta tttaaaagtt
      601 catcctagag atgtagtaga ttattccaaa ttgccggtag agctattacc atcaaatgtt
      661 cctatggaaa ttatagagtt gatgtcaaca ggtcggttcg aatgtgggat aacacattcg
      721 tccactgcgc tggatttttt aacttgtgtt gataaaaaaa taactttagt agatctgaag
      781 gatattaaat gaatactaag aaattacttc aatctggttt tatttataca ctcgggaatt
      841 tattggtgca aggattagcc tttatcacac ttcccatcta tactcgggtt atctcaacag
      901 aagtttatgg ccaatatagt ttatatgtcg cttggatgaa tattattatg ctttttattg
      961 gccttcagac gagcggttct ttaagttcag cccgggtcaa atacggagaa gaatttaaaa
     1021 gctattcagg gagtgccttt tctgtaggta atatatggtt tcttattata cttttgatag
     1081 cttttctatt tagaagtttt cttgcaccat tagttggttt ttctgaatct atttttttat
     1141 taatggtgtg tcaaagttac gctagctatg tggtgacttt ctttggtcag tattttatac
     1201 aacaacagag gagtttggct aatttaatat tatccttagc caatgcagtt tcatctgttg
     1261 cactatctct atttttaatt tttcattggt ccgatgactt tttatctagg gtttttggag
     1321 cttttgttcc tactataata actggaatag ttgcctttgc ttatatttat tatcatagca
     1381 aatcttttta caatcctaag tattttcggt tcattgtcac tgtgtctgtt cccttgattt
     1441 tccatttgtt aggacaccag ttgttggggc aattagatag aattatgcta gctcgtttat
     1501 acaatactaa agaagttgca atgtatagtt tcggttattc gcttggaatg attattcaga
     1561 ttgttttgaa tagtattaat atggcttgga ttccatggtt ttttgatgct agaaaagaaa
     1621 aattactgca actttcaact tatattagcc gttatctcta cttaggagtt ttcttaacat
     1681 taggttattt gactgttttt cctgagttag ctcaaataat gggaggagac aagtatagta
     1741 gtagtgttca atttatttct ttaattattg tcagctactt tttagttttt ctctatacat
     1801 ttcctgtaaa tatccaattc ttctatgcta atacgacatg gattcctatt gggacattat
     1861 tagctgctgg tgtaaattgg ttactaaacc ttgtattaat tcctcattat gcagcctatg
     1921 gcgctgctat ggctacaata atatcttatt tagccttgtt gatttttcat catattgttt
     1981 cgaaagttaa ataccattat tcagatgtct cagttaggca gtacatcatt ttaagtggta
     2041 tagtatttag ttatgctatg ttaatgaaca tgttcctaga tactatcgtt attagatgga
     2101 gtttaggaat aattattctt atagtatata gcattgtttt tcaaaaagtc attttagact
     2161 tattgagtaa aaaaagaagg agaagataat ggtttatatt attgcagaga ttggttgcaa
     2221 tcataatgga gatattaatc ttgcgaaaaa aatggtagat gttgccgtgt cttgtggtgt
     2281 tgatgctgtt aaatttcaga cttttaaagc tgagaaactt atttctaaat ttgctcccaa
     2341 agctgaatat caaaaagcaa ctacaggaac agcagacagt caacttgaga tgacgaaacg
     2401 tttagagtta agctttgaag aatacttaga aatgcgtgat tatgcaattt caaaaggtgt
     2461 ggagaccttt tcaacacctt ttgatgaaga gtcattagag ttcttaattt ctacagatat
     2521 gccaatttac aaaattccat caggagaaat cactaattta ccttacttag aaaagattgg
     2581 caagcaacaa aagaaagtta ttctttcgac gggtatggcg gtaatggaag agatccatca
     2641 agcggtgaat attttacgtc agaatggtac aaccgacatt tctattttac attgtacaac
     2701 agagtaccca acaccttacc cctctctaaa tttaaacgtt attcatactt tgaaagatga
     2761 atttaaagat ttaacgatag gttattcgga tcattcaatt ggatcagaag tacctatcgc
     2821 agcagcagca atgggtgcag aagttattga aaaacacttt actttagata ctaatatgga
     2881 aggtccggat cataaagcca gtgcaacacc tgatatttta gctgctttag ttaaaggggt
     2941 tcgcattgtt gaacaagcct taggtagatt tgaaaaaatc ccagatccag tagaagagaa
     3001 aaataagatt gttgctcgta aatcagtcgt tgctttaaaa ccaattaaaa aaggcgatat
     3061 ttattcaata gaaaatatta cggtgaagcg cccaggtaat ggtatttctc ctatgaactg
     3121 gtatgacatc ttgggacaag aagcgcaaga tgatttcgaa gaggatgaag ttattcgtga
     3181 ttcacgcttt gaaaatcaat tgcccgagtt ataataacac ctaattataa tcggcaacag
     3241 atgtattaca cggctaagat tatgtaatta ctatatatag aagggatacg atgaaaaaaa
     3301 tttgtttagt aaccggatcg cgtgctgaat acggaataat gaagccattg attcaaagat
     3361 tatcaaaaga taaagaagtc aacttacaaa ttattgcaac agcaatgcat ctggaagaaa
     3421 agtacggcta tacttatcgt caaattgaag aagacggttt tgatattgct tataaagttc
     3481 ccttacatct ttatgatact gacagaagaa ctgtatctac tgcaatggcg catttacaac
     3541 taggattgac caaaattttt gacaaggaag actatgatct agtcatcatt ttaggggatc
     3601 gttacgaaat gttaccagtt gtgaatgtag cgttgattta taatgtccca gtatgccacc
     3661 ttcatggagg ggagacctca ttaggcaatt ttgatgagta tattcgccat gcaattacta
     3721 agatgagtca cctacactta gtctctacag aggactttcg tcaacgtgtg attcagatgg
     3781 gagaacaacc tcaatttgta attaacacag gagctctcgg agtggaaaat gctctatcaa
     3841 tcccttctct aaccaaagaa gcaatagaaa agcaattagg tattgtacta gaggaatcct
     3901 attttgttgt tctctatcat cctgttactt ttgaacaagg aaaaagtgct ggagaacaga
     3961 tgaaagcggt actatcagcc ctttctaaat ttggtgtcca gtgtctattt ataggatcca
     4021 attcagatac tggttctgat gatattgcaa aggctatcaa tacttatttg ataaatcatg
     4081 aaaatagtta ttgctttgct tctttatcaa cccaacttta tcattcctta attcgtcatt
     4141 cactaggact gattggtaat tcgtcttctg gtttaataga agttccttct ttgatgaaac
     4201 ctactcttaa tattggtgat cgccaaaaag gtaggctaca tggagaaagt gtagtaagtg
     4261 taccagtcga aaccccctct gtattggagg gattatcaaa attgaatgaa gtgaccaact
     4321 ttgacaatcc ctactacaaa gagaatgctt catctattgc atatgaagca ataaagcttt
     4381 atttgaaaga tgaaccttca atttcccaac cattttatga tttaaaggag aataatctaa
     4441 aatgaaaaaa aagttggcat ttttaggcgc aggaagtcat gctgatgcga ttgctccggt
     4501 tattgatcct cttgtgtatg atttcgtagg tttttttgat gataaagata ttacggagca
     4561 tgatggttat cctgttcttg gaaaacttta tgatgtgcta ccttaccttg aagatggctc
     4621 aatagatgca gtatttatta caataggtga taatgctaaa aggaaagaac tatttgaata
     4681 tgtagcaaag gattattatg actttattat taacatcatt agtcccaatg ctttagtatt
     4741 gacaccagat agtatttgtg ggcgtggtat ctttattggt tttggggctt ttataggttc
     4801 taaagtgaag ctgtttgata acaacgttgt taatacagga gcgctcattg aacatcatac
     4861 tgttgtagaa tcacactgta atatagcacc taatgctacc ataaatggtc tttgttatat
     4921 tagagaagaa gtttatgtag gtagtgccag tgttattatt caaaccttgg atatttcatc
     4981 gtgtacgaca attggcgcag gagctgttgt tgtaaaagat attatagaac ctggaaccta
     5041 tgtaggggtt ccagccaaaa aaataaaata aaggatatgt tatgaagcca atttgtatta
     5101 ttcctgcgcg atcagggtca aaaggattac cagataagaa tatgttattc ctagcaggta
     5161 aacccatgat tttccatact atagatgctg caattgaatc tggaatgttt gataagaaag
     5221 atatttttgt tagtacagat tcagaattgt acagagagat ttgtttagaa cgcggtattt
     5281 cagtggtgat gagaaaaccg gaactttcaa ctgatcaggc aacttcgtat gatatgttaa
     5341 aagatttttt atctgactat gaagataatc aggagtttgt gttacttcaa gtaacctctc
     5401 cactaagaaa atcatggcat ataaaggaag caatggagta ttattcttca catgatgttg
     5461 acaatgttgt aagtttttct gaagttgaga aacaccctgg tctgtttacg acattgtctg
     5521 ataaaggcta tgctatagat atggtgggag cagataaagg ttatcgtcgc caagatttac
     5581 aacctttata ctatccgaac ggcgctattt ttatttctaa taaagaaact tacttaaggg
     5641 aaaaaagctt tttcacctct aggacatatg cttatcaaat ggcaaaggaa ttttcattag
     5701 atgttgatac gagagatgat tttatccacg tcatcggtca cctctttttc gattatgcca
     5761 ttagggaaaa agagaataaa gttttttata aagaaggcta tagtcgtttg ttcaatagag
     5821 aagcttcaaa gataatttta ggtgattcaa aaacgatatc tatctcacta gaaaattacc
     5881 ataattattc ccaaggtggt gtaacattag caacgatgtt ggaaaactta cctaactttt
     5941 tgacagctaa tgtaacagag gcttttgttt ctataggggt aaatgatctc attacaggtc
     6001 atagtgtgga agaaatattc agcaatttcc aaaaactcta ctcattatta gctgagaata
     6061 aaataaagat gagattgaca actattgctt acaccctttt tagagaaact gtcaataatg
     6121 cagatattga gaaaatcaat caatggctaa cagaattttg ttatcaaaat cagattccac
     6181 tgttagatat taatagattt ttatcaaagg atggtaatct aaattatcat ttaactagtg
     6241 atggattaca ttttactcaa gaggctaatg atttgttaca aagtcaatat caattatttg
     6301 tagacgaagt taaaacctta taatatttat tttataaaat tattcatctt atttttgcga
     6361 tttcaatagt gatatggtat atttaaagta agctacgtat agttttacca gtcagaaata
     6421 gtaaggatgg ttgagtatga gagaaactga aacacacgat caccaagcgc taattcaaaa
     6481 attgttagtc agtattcatt atttaacctt gtttcgtgat gaaattatat tagtagaaaa
     6541 aaccccatct ttactgggga aacatttttc tattgctatt gtgcagaatg aactaggtga
     6601 gatactttct aaaatagaag ctttgagtaa gcaaaaaaaa ctgattcgtt ctatttattg
     6661 gtatgatgaa tcgtcattta aagtcatgaa taaggctctg gctattgtag aagaatggat
     6721 taagggctta gataatcttt tagaattctg tcaatcacag acggtttttc aagcaatttt
     6781 aggtgatgaa cgtgcacatg tatttggaat ccttattgat gtttatactt ctttaaacat
     6841 tataaatact tctttgaaag aagaaaattc tagaccagtg tccttatctg atttggctct
     6901 aggtcttaag gacgaagatt aataaaaaaa cgttactgct ttgaatgaat aaagcagtaa
     6961 cgttttttat aaaatattgc tatactagta atacagatat ctaaaagtaa ggagttataa
     7021 atgaaacatt ccagttggca tgatttaata aaaagagaac tacccaatca ttactacaat
     7081 aaaattaata catttatgga tgcagtttac gaaagcggaa tcgtttatcc tccaagagat
     7141 aaggtgttca atgccataca gataacacca ctagaaaacg taaaagtagt gattattggc
     7201 caagatcctt accatggccc tcaacaagct caaggactct cattttctgt tccagacaat
     7261 ttaccagctc ctccttcact tcaaaatatt ctaaaagaat tagcagaaga tattgggtca
     7321 cgttcacatc atgacttaac atcatgggcg caacaaggag tgctgctatt gaatgcttgt
     7381 ttaacagttc cagaacacca agctaatggt catgctgggc ttatatggga accttttact
     7441 gatgctgtca tcaaagtggt taaccaaaag gaaaccccag tagtctttat tctgtggggt
     7501 ggctatgctc gaaagaaaaa gtcgctaatt gataatccta ttcatcatat tattgagagt
     7561 cctcatccga gtcccctatc tgcttatcgt ggtttctttg gtagcagacc tttttctaga
     7621 acaaatcatt tcttagaaga agaaggaata aatgagattg attggttaaa ttagaaggga
     7681 aatctactct cgaaacattg ttagtcttat aataaaaaac cgctagtagc ggttttttat
     7741 tatttatttt tttgtttact taggtttaag ccaaacggaa ccaaattttc ttgccttttt
     7801 cgaatacgtt tgatattatc ctgatgtcgg ataataatcg tgagggccat taagataacc
     7861 acagtggtaa atatccagtc atagtccgtt aatataaaac ctactaatgg aaaaattaag
     7921 acactaagga tacctacgac agctactgta atactggata gcgaaatcat gctaaaaaga
     7981 taaagcgtta ataaaaagat cactaataag taaaggaaaa aagaaggtgc aaatcctagc
     8041 aaaaccccag cgcttgtagc aacggctttc ccacctttga attgtgcaaa aatagggaaa
     8101 gtgtgaccta taatagcaaa aaaaccgata aaaaatggag atactgtcgt tacacctaat
     8161 atgataggaa taagtgttgc tagagtccct tttaaaatgt caatagtcaa agtgacaatg
     8221 ccagctttta cacctaaaat acggaaggtg ttcgtggtgc cagtattacc actaccgtgt
     8281 tgacgaagat tgacttggta aaaatattta ccaatccaaa gcccagtttg gatagagcct
     8341 aacagatagg caatgataat cataattata atattcatac ccttattata acataagcat
     8401 aaaagtcttt ttgctatttt taaaaaaata attttgcaaa aacagcaaaa aacttgtaat
     8461 ataaaaaaga tgaaaaaatt tggaggtcac tttgactaaa caagatataa cagtgacgaa
     8521 ttatggcgac gatgctattc aagtcttaga aggattagat gctgttcgta agcgtccagg
     8581 gatgtatatc ggttccacag atggaactgg tttacatcat ttagtgtggg aaattgtaga
     8641 taatgcagtt gatgaagctc tatcaggttt tggaaatcgc attgatgtga ttattaacaa
     8701 agatgggagt ataactgtta cagaccatgg acgtggtatg ccaactggaa tgcatgctat
     8761 gggaaaacct actgttgaag tcatcttcac ggtgcttcat gcaggtggaa aatttggaca
     8821 aggtggttat aaaacatcag gtggtctcca tggtgttgga tcatctgtag ttaatgcttt
     8881 gtcttcttgg ttagaagttg aaatcattcg cgacggagct atttacagac aacgttttga
     8941 aaatggtggt aagcccgtta caactctgaa aaaaattggc acggcaccaa aatcaaaatc
     9001 tggaacttct gtcagcttta tgccagatca atcagtattt tcaacgatag attttaagtt
     9061 taatacaatt gcagagcgct taaaggaatc tgcctttctc ttaaaaaatg ttactttaac
     9121 cttaacagat aatcgttcag aagaagcaga gcatctagaa tttcattatg aaaacggtgt
     9181 tcaagatttt gtagagtatt taaatgaaga taaagaaacg ttgacaccaa tcatgttttt
     9241 cgaaggtgaa gaacaagaat ttcatatcga agttgctttg cagtataatg atggtttttc
     9301 agataatatc ctttcttttg tcaataatgt tcgtactaaa gatggtggaa cacatgaaac
     9361 tggcttgaaa tcagctatta caaagtctat gaatgactat gcacgtaaaa caggacttct
     9421 taaagaaaaa gataaaaacc ttgaaggttc tgattatcgt gaggggctat cagccatttt
     9481 atctattctt gtacctgagg agcacttgca atttgaaggt caaactaaag ataaactagg
     9541 aagtccctta gcacgaccaa ttgttgatgg tattgtttct gaaaagttaa cttactttct
     9601 tatggaaaat ggagatttag catctaattt aatccgtaag gcaataaaag cacgtgatgc
     9661 gcgtgaagct gcacgtaaag cacgtgatga aagtaggaat gggaaaaagt cgaaaaaaga
     9721 taaaggtcta ctttctggaa aattaacccc tgcacaatca aaaaatgcta agaaaaatga
     9781 actctattta gtcgaaggag actctgcagg tggctctgct aagcaaggtc gtgatagaaa
     9841 gtttcaagct attttaccgt tgcgaggaaa agtattgaat acagctaagg caaagatggc
     9901 tgatattata aaaaacgaag aaattaatac aatgatccat accattggtg caggtgttgg
     9961 tcctgatttt aatcttgacg atattaatta tgataaagtt atcattatga ccgatgctga
    10021 tactgacgga gctcatattc agacactatt attaactttc ttttatcgct atatgagacc
    10081 tcttgttgaa gagggacatg tctatattgc tttgccacct ttgtataaaa tgtcaaaagg
    10141 aaaaggtaag aaagaaattg ttgagtatgc ttggacggat atagagttag aagaattacg
    10201 acaaaaattt ggtaaaggct cactattaca acgatataaa gggcttggag aaatgaatgc
    10261 cgatcaattg tgggaaacca ctatgaaccc tgagacaagg acattaattc gtgtaacaat
    10321 tgaagattta gcaagagcag aacgtcgagt taatgtcttg atgggtgata aagttcctcc
    10381 aagacgccaa tggattgaag ataatgtcaa gtttacttta gaagagaata cagtatttta
    10441 ataaagagcc ttggttattc atgttttata gtgtacaagt aatattgtat gcgttagaca
    10501 agagagccaa tcattcattt caaataagtg gaaaaataat actaagccac tccaactagg
    10561 agaaaattaa aaatatgtct aatattcaaa atatgtccct tgaggacatt atgggagagc
    10621 gttttgggcg ctattctaag tatatcattc aagaacgggc gttgccggat attcgtgatg
    10681 gcttaaaacc tgttcaacgt cgaatccttt attcaatgaa taaagatggt aatacttttg
    10741 aaaagggatt tcgcaaatct gcaaaatcag tcggtaatgt tatggggaat ttccatcctc
    10801 atggggattc ctctatctat gacgcgatgg ttcgtatgtc tcaagattgg aaaaatcgtg
    10861 agacattgat tgaaatgcac ggaaataatg gttctatgga tggagatcct gcggcagcta
    10921 tgcgttatac ggaagcccgt ctatcagaaa ttgctgatta tcttcttcaa gatattgata
    10981 agaatactgt accctttgcg tggaattttg atgatacaga gaaagaacca actgttttac
    11041 cagctgcctt tcctaatctt ttagtcaacg gagcaacagg aatatctgca ggctatgcga
    11101 cagatattcc accacataat ttagcagaag tcattgatgc tgtcgtgtac atgattgatc
    11161 accctaaagc taaattagat aaattaatgg aatttctacc tggtccagat tttccaactg
    11221 gcgctatcat tcaaggaaaa gatgaaattc gtaaggcata tgagactggt aaggggagag
    11281 tagcggttcg ctcgcgaact gctattgaaa ccttaaaagg tggtaagaaa caaattattg
    11341 ttactgaaat tccttatgaa gttaataagt cagttttagt aaaacgaatc gatgatgttc
    11401 gtgttaataa taaagttcca ggaatagcag aagtgcgtga tgagtctgat agagatggcc
    11461 ttcgtatcgc tattgagctc aaaaaagaag ccgatgaaac aattgtctta aattatcttt
    11521 ttaaatatac agacctacaa gttaattata atttcaatat ggttgctatt gatgactata
    11581 cccctaagca ggtgggtcta tcaagaatat taactagcta tatcgctcac cgaagagaaa
    11641 ttattattgc tcgttcaaaa tttgataaag aaaaagccga aaaacgtctc catattgttg
    11701 agggactgat tagggttctt tctattttgg atgaagtgat tgcattaatt cgtgcttctg
    11761 agaataaagc tgatgcgaaa gagaatttga aggtcagcta tgagttttca gaagctcaag
    11821 cagaggctat cgtcacattg caattatatc gtctcacaaa tactgatatt gtgacgttga
    11881 gagaagaaga agaagaactt cgtcaacaaa tcacaatgct caaggcaatt attagtgatg
    11941 aacgtacgat gtataacgta atgaaacgtg agttacgtga ggtcaaaaag aaatttgcca
    12001 atactagacg ttctgaactt caagaattag ctgaaactat tgaaatcgat actgcaagtt
    12061 taattatcga agaggacaca tacgtcagtg ttactagagg cggatatgta aaacgcacca
    12121 gccctcgctc tttcaatgca tcaaccgttg atgaacttgg taagcgagaa gatgacgaat
    12181 tgatatttgt gagcaatgca aagactaccc aacatttatt gatgtttact aaccttggta
    12241 atcttgctta tcgtccagtt catgaattag ctgatattcg atggaaagac gttggtgaac
    12301 atttatcaca aaatcttgtt aactttgctt caaatgaaga aattatttat gctgaattag
    12361 tagatgattt tactaaagaa acttattttg ctgtcactag tttagggcaa ataaagcgtt
    12421 ttgaacgtca agaaatcagt ccatggcgga catataagtc aaaaacagct aaatatgcta
    12481 agttaaaaaa tgtagatgat tacgttgtga ctgttgctcc tatccaatta gaggatgtta
    12541 tacttgtcac ttataatggt tatgtgttga gatttagtat aaatgatgta ccagtcgttg
    12601 gtagtaaagc cgcaggtgta aaagcgatga atttaaaaga tagtgatcac atcgtatctg
    12661 catttatagc taacaccaca agtttatatt tattaactca tagaggaagt cttaaacgaa
    12721 tggccattga tgttatcccc acaactagtc gtgctaatcg aggacttcaa gttcttcgtg
    12781 aattgaaatc caaaccgcat cgcgttttta aagcaggacc tgtatattta gaggattcat
    12841 cttttgaatt tgatttattt agttcagtat cgaatcatga aggagatact ttcgtacttg
    12901 agattatgtc taaaacagga aaagtttacg atgttgattt aagtcaatgg agtttttctg
    12961 aacgtacaag taatggctct tttgtatctg ataagatttc tgacgaagaa gtattctcag
    13021 ttaaaattaa gtaaaaacat atttgtgttt ttactagttt ggtaatttat ctgaatttta
    13081 caaataatat tgaaaaaata atgataatag cgtacaatag ttagaaaccc aaagaaagag
    13141 gtaccttatg acagtaaatt tagattggga taatttaggt tttgcttaca gaaaattacc
    13201 attccgatac atttcgcatt ttaaagatgg gaaatgggat gatggcaagt tgacagatga
    13261 tgcaacgcta catatttcag aaagttctcc tgccctccac tatgggcaac aagcttttga
    13321 aggattgaaa gcttatagaa ctaaagatgg ctctattcag ttatttcgtc cagatcaaaa
    13381 tgcagaacga cttcaaagaa cagcagatcg tcttttaatg ccacatgttc cgacagacaa
    13441 atttattgca gcggttaaat ctgttgtacg cgctaatgag gagtttgttc caccgtatgg
    13501 tacaggggca acattataca ttcgtccctt attaattggt gtgggagata ttattggagt
    13561 taaacctgct gaagaatata tttttacggt atttgcaatg ccagtaggta gttattttaa
    13621 aggtggttta acaccaacta actttattgt ttccaaagaa tatgatagag cagcaccgaa
    13681 tggtacagga gctgcaaaag ttggtggtaa ctatgctgct agtcttcttc ctgggaaata
    13741 cgctcatgag aaacaatttt cagatgttat ctatcttgat ccagcgacac atactaaaat
    13801 tgaagaggtt ggagcagcta atttctttgg aattactaaa gataatcagt ttatcacccc
    13861 tttgagtcct tcaattttac cttcaattac taaatattca ttgctttacc ttgctaaaga
    13921 aaggtttggt atggaagcga ttgaaggaga tgtctttgtc gacgaattag ataaattcac
    13981 agaagctggc gcatgtggta cagccgcagt tatttcacca attggaggaa ttcaaaacgg
    14041 agatgacttc catgtcttct acagtgagac agaagttggt ccagcgacac gtaaactata
    14101 tgatgagtta gtagggatcc agtttggtga tgttgaagca ccagaagggt ggatttataa
    14161 agtagattaa gtctatacta aaagcttgct cttgcaagct tttagtattt tgtgtacaat
    14221 aaaaaacaat aaaagagata ttggagttaa tagtgagtaa aaaagataag aaaattgaaa
    14281 ttcaaatttt agacgctcaa gtaacagtta atggaactaa ggttgatgga taccaactcg
    14341 ttattggaaa aaaaattgta ggtcaaattg cagagctaga ttctaaattt gctgtgatta
    14401 aaggtgagca agtgagtgga ttctataaaa atttagatca agctatggga aatattattg
    14461 aagaatataa tcttaatcgc taatatttat ttaaaaagct tgcgtgctaa caacatctgt
    14521 ggtagaatag tcagtgttgt tacggaaaga tagcgaagag gctaaacgtg gcggactgta
    14581 aatccgctcc ttcgggttcg ggggttcgaa tccctctctt tccattattt ggggtatagc
    14641 caagcggtaa ggcaagggac tttgactccc tcatgcgttg gttcgaatcc agctacccca
    14701 gtcaaaggta tcaaactttt gtttgacacc gtcaaagaga tatttgttgt ccttgcgggt
    14761 taccttgaga aaatatattg ttatcaaaag ctagctcggc tagcttttgt tggaggattt
    14821 tttagaatga atgaatttga agatttgcta aacagtgtta gcgaagtgaa tcctggtgac
    14881 gttgtcactg cggaagtttt aacagttgat aacggtcaag caaacgttgt tatcgatggt
    14941 actggtgttg aaggtgtctt gacacttcgt gaattgacta acgatcgtga tgctgatatc
    15001 aatgattttg ttaaagctgg cgacacagtt gaagtacttg ttcttcgtca agttgtaggt
    15061 aaagatactg atacagtaac tttccttgta tctaaaaaac gcttggaagc tcgtaaagca
    15121 tgggacaaac ttgttggtcg tgaaggtgaa gttgttactg ttaaaggtac acgtgctgtt
    15181 aaaggcggac tttcagttga atttgaagga cttcgtggtt tcatcccagc ttcaatgatt
    15241 gatactcgtt ttgtacgtaa cactgaaaaa tttgttggac aagaatttga tgctaaaatc
    15301 aaagaagttg atgcagcaga aaatcgcttc atcctttcac gtcgtgaagt tgttgaagaa
    15361 tcagcagctg ctgcacgtaa agaagttttc tcaaacattg aagtaggttc agttgtaact
    15421 ggtaaagttg ctcgtttaac aagctttggt gctttcattg accttggtgg tgttgatgga
    15481 cttgtacacg taactgaatt atcacatgag cgcaatgttt cacctaaatc agttgtaact
    15541 gttggtgaag aagttgaagt taaagtactt tcaattgatg aagaagctgg tcgtgtatca
    15601 ctttcactta aagcaactac acctggccca tgggatggcg ttgaacaaaa acttgctgct
    15661 ggtgatgtta ttgaaggtaa agtaaaacgc ttgactgact ttggtgcttt tgttgaagtg
    15721 ttacctggca ttgatggact tgtacacatt tcacaaattt cacacaaacg tgttgaaaat
    15781 cctaaagacg tattatctgc aggacaagaa gttactgtta aagttcttga agttaattca
    15841 gatgcagaac gtgtatcact ttcaatgaaa gctcttgaag agcgtccagc gcaagcagaa
    15901 ggtgaaaaag aagaaaaacg tcaatcacgt ccacgtcgtc cacgtcgtca agaaaaacgc
    15961 gactatgagc ttccagaaac acaaactgga ttctctatgg ctgacttatt cggtgatatt
    16021 gaattataat aattaaaaga cttgctaaag caagtctttt tgtttttgtt gataaagtat
    16081 gctataatgt aacatgtact accatcttta gtgtaatgga tatcacacaa gattccggtt
    16141 cttgggatag gggttcgatt cccttaagat ggataataga tgctccataa agctttatgt
    16201 tataggcttt atattagttt atctccaacc ttgcctcaca gactgattct cctcatttct
    16261 gataagtaaa actagggttg acaaataagg gagaatcagt ttgaaatctg tgtaagttag
    16321 tatcataatt agttagtatg accattaagg ggctaagtta aggtactaag attaaaccat
    16381 tgtgtaataa aatgtttcta ataagtaatt aaggtattaa taaaaaggcg ttaatgcctt
    16441 tttattttaa accatgctgt tgtttgttag tgagtttatc attttcaaat gtaaggttaa
    16501 ttcccgggtc actgtctgac gactggatgc cactaatcca tgcagcctgt agttcctctt
    16561 tatctgaaga aactgcttga gaaatatcat caggttctcc tagaagttct tttactttat
    16621 tgtatgacat gccatttttt aaactattat aatcttttgt tgtgatgttg gcatcacgaa
    16681 taaacttaaa attactaatt gaacgaacaa tgctactatc gtttaataat tgtatagtga
    16741 tggagatatc atcgacattc caacgatagc ctttaagagt gacatttccc gctggtgtat
    16801 caaatttttc atttggttcg ccaccaaata attgttttaa ttcagcgaga ctagtgcctc
    16861 ctttaaagtt atttgcttta acccctaact ttactttatt aaatttcaaa cgtaattctt
    16921 cgtggtcagc tttgatattt ttatgttgtg atacatttga ttttgaagta gaggtttgct
    16981 gagtatttga agaagagcat gctgttaaac ccaaaaatga gagacagaca atcgttccaa
    17041 cagttatttt ttttaacggt atcataatta tttatcctaa attctatttt taaatattat
    17101 aacataatta ttttgggttt acttttcttt tcctcatttc aatattaagc ctgcaactaa
    17161 aggactgaca ataacatagc aaattcctgt gataccaatt gctagtccag ccatggcgcc
    17221 ttgaacttgg ccatatttta aggcttgtcc agttcctata gcatgcccag ttcctcctag
    17281 cgcgagccct atagcaacag gatcttctat ttttaataat tttagaaaaa taggccccaa
    17341 aacgcttgtt aagatgcctg taataacaac tacgaccaga gtgatagtag ctaatccacc
    17401 tgctttactt gtaattccaa cggccatagc tgtagttacg gactttggga aaagtgagat
    17461 tgctaagaaa tatttcatac caaaaaactt agcaactatt gcggtaaaaa ctgtgttgat
    17521 aacagaagct aagatgatgc taatgctaat acttttaatg tgatgcttca ttaaatgaaa
    17581 ggttctatag agaggaatag ccaatacaac cgttgaaggc gtaattaaca tcgttagaaa
    17641 tgaaccacca ttgtagtaat ctttatagga gatatgtgtt aattttaaaa aagcaattaa
    17701 taaaatagtc gccaaaagaa gtggagttgt tgcaggggaa ggatattttc taaaaatcct
    17761 tatgccaatg taatacgccc atacggttaa cataatacca aagatagggt tattcgttaa
    17821 agttgccatt tttacctcca atttctggat aatctccttc atatctcttt ttgatccatt
    17881 gggataccag tccaatagtt agaatattga gaaatagcgc tcctaagata atgatagcta
    17941 tcccaaaaag atagggcttt atgtcttgaa agtgctccat cagtccaact gctggtggta
    18001 aaaataaaat ggtcatattt gctaatagaa aattccccac agtatcaata tgcctgagcc
    18061 tgatgatttt gtattttaac gctaggaata gtaatattaa tccaataata ctacctggta
    18121 taggtaagtt gaatatagta gatagaacct caccagcgaa tgaaaaggtt aaaataacca
    18181 ttaattgaac gtataatttc atgaaaaact ccttccatta tagtctacta ctctgaaagt
    18241 aattttcaag gctatgaaac gtttacataa aaacaaatta aaaagacgga gattcttctc
    18301 aatcttttta atttgttttt gtaagctatt tttatcaaga gtaactgatt aaatagtata
    18361 ttaatcaagg gaagtgattt gatatcgtct cgttttatac ggacttgact tggcagttat
    18421 ttgatggtag attaagaacc atttttcatt acggcgccaa atactagtat gtcgtttacc
    18481 ttccaattca taagacacaa gttttgtttt ttgactaatt gattttacat tgaaatattt
    18541 aaagttgctt gccaatgaat gatttgtagc attagtaagc atgtcagaag tgataatatt
    18601 tcccttttca ttaatcatat aaaagtcagg gctaataaaa gtctcataac cggggatttt
    18661 tgctaggatg attttttcgt ttttgtataa tttttggtaa acattatcga aaatttcgtc
    18721 ttctgggata gaagcgggtt ctacatgaat atctatgtca tatacagaaa atttatcact
    18781 gagtagtttt tccacttttt cggtaatggc atgactttca aagactgaga gatcagggtt
    18841 catttcgaga acaatatcaa ggtaaatatt actaccgtaa gtacgtccac gttgagattt
    18901 tacagcggat attttaggaa ttgttaatat agctttttcg tattgtttta attgataatc
    18961 atcgaaacca tccgataagc tgaaagcaga ttctataaaa atatcataag ctgttttaag
    19021 aataaaataa gtaataatga tagcagctaa acgatcaata ataggaaaat ttagagaagc
    19081 cgcaatgata gcaatagatg tgccgataga tgtcacagca tcagaaaggt tatctttaga
    19141 agctgcaacc agagcgctag actttactct ttgagataat tgtttattat agaaacaaac
    19201 gccaagcata acaagagcag atatagcacc tactatagca cctagaggat caatgttagt
    19261 atcagtatta ttaataatct tagtgacagt ttgaataaaa acttgaatac ctacgacaaa
    19321 catgataaaa gaggtaatca ggcttgctaa atcttcaatt ttccagtgac caaaacgatg
    19381 gtttgagtcg gctggttgac tagccaagtg taaaccaatt aagagtgcta catttcctaa
    19441 gatatcagat aagttattaa atccatctgc tacaagtgaa gtagcatcaa accaataacc
    19501 tgcagctaat ttagcgactg ccagtgttat ataggcaatg atactaataa taggccctct
    19561 ttttgcaagt tgtaagtttt cagtagaagt tgccattttc ctaatttcct ttcatctaca
    19621 cattatatca cttaggagca gactataaaa ctagtaagtt caaggcttag tgatattttt
    19681 agtctctgaa aaatatttct gtgtttcttt aacaataatt ggtgacaaag ctaggagagc
    19741 tattagattt ggtaaagcca ttaaaccatt aacgatatca gcgataaccc aaatgagatc
    19801 gagctgtaag aaaccaccta agccgaccat aatgacaaaa ataatgcgat aagcgttaat
    19861 aaatttagtc cccaatagat attcaaagca acgctctccg tagtagctcc atcctaaaat
    19921 agtagtaaaa gcaaataaga caaggcagaa ggttagggca aaactaccta gtgagccaaa
    19981 tacggtatta aaagatgctt gtgttaacgg agcaccctgt aaatcaaatt ctagccattt
    20041 tcctgtaact aaaattgcaa tccctgtcaa agtacaaata acaatagtgt caatgaaggt
    20101 tcctgtcatt gaaataagcc cttgttcaac aggttctttt gttttagctg ctgctgcagc
    20161 tattggagca gaacccaatc cagattcatt tgagaaaact cctctagcaa ttcccttttg
    20221 aattgcttct ttaacgatag ctccagaaaa accaccaatg gctgctgtcc cagaaaaagc
    20281 accgctaaag atgagttgga aagtatgtgg aatcttattg taattaactg caataataat
    20341 cagagttgct aaaatatagc ttatagccat aaaaggcact attttttctg aaacttttga
    20401 aatagatttt atacctccaa atataataaa taatactata acagcaagaa tgaggctggt
    20461 aaatctagtt gatattttga aagtatgctc aagagaagag gcaattgcat tgacttgagt
    20521 gaatgtccct attcctaaaa gagctactaa gatacctgct gcagaaaaaa agactgctaa
    20581 tggtttccat ttttgtccca ttccattgac aatgtagtac attggtcccc cagaaatttc
    20641 tccattagta tcttttgtgc gatatttgat agctaataat ccttctgcgt attttgttgc
    20701 cattccaaaa aaggcggcta cccacatcca aaacaaagct cctggtcctc cagatttaat
    20761 agccgtagcc acaccaacga tattacctgt ccctaccgtt gcagcaagag cagttgcaag
    20821 agcagcataa cttgaaatat ctccttgtcc attatcttct cggaaaatca atttgaaagc
    20881 tctagaaagt tgtctcaatt gaacaaaacc taagcgaaat gataggtaaa tacctgttcc
    20941 gactaataaa gcaagtaaag gtggacccca aacgaagcta ttgatatgag taaaaagtgt
    21001 tagcatatgt tctcctttga tacatgacat acctatggat tctgtaatga aacgaaagaa
    21061 gttatctcta aaagagataa cttctgaaag atgctagcta aaaagcgcac aaataaaggt
    21121 agtagtaaac ttaataagct tactactcat tattaatatt ggcttttagc ttctgtcctt
    21181 ttgcctgaga gattggagga gttgttttac tcttcttgcc ccttcggcgc ctagagcaca
    21241 tcttggtgtc aatacaaaga tgttgctact tataggtctc tccagaagtc cgtctgtcaa
    21301 tcagtcctgt cttaagacac ctgagagtgt aattccttcg gcgaaagtct aactttcttc
    21361 tcctgaatga cgtcattcgg tatattttat tatgttaact atcataatga aaaagcatag
    21421 ttttgtcaat atagtttcaa aaaactcttg ttttctaaaa tgtcttatgc tataattctt
    21481 tcagcgtagg tttttaaact catcccgtga ggtgagaaaa acatcaggaa aaaaataaag
    21541 cctatgacta tattgctata gaaagtgggc ttttttgtat gcccaagagg actagtttag
    21601 atatgacttt aaatgctaaa ggtgaaagta atcaaattct agtagataag gaagtacatt
    21661 ctactaactt attactagat atcgatgaaa aaccagaatt atttcaaggt ttgcttttaa
    21721 gttttcaaca tgtttttgcc atgtttggag ctacgatatt agttccgctt attttaggga
    21781 tgcctgtatc agtggcttta tttgcttctg gttgcggaac gctcatttat caagttgcga
    21841 caaaatttaa agtccctgtt tatctaggct catcatttgc ttatattaca gctatggcat
    21901 tagccatgaa acaaatgcat ggcgatattt cggctgctca aacaggtatt ctttttgtag
    21961 gtctaatcta tgtcgttgtg gcgacggtta ttaagttcgt aggaaattca tgggttgata
    22021 agattttgcc accaataatc attggaccta tgattattgt tattggttta ggtttggcaa
    22081 actctgctgt aactaatgca ggttttgtcg ctaaaggtga ttggcgtaaa atgctagttg
    22141 ctgttgttac attcttgatt gcagctttta tcaatactaa agggaaaggt tttatcaaga
    22201 ttattccatt tttatttgca attattggag gttatatttt atcaatcatt cttggcttgg
    22261 tagatttatc tccagttgaa aaagctgcct ggtttgaatt accaaaattc tatttaccgt
    22321 ttaagacagg tttatttcac agttataaac tttactttgg accagaaatg cttgcaattc
    22381 ttccaatctc aattgtaacg attgctgaaa atatcggtga tcatacagtt ttaggtcaga
    22441 tttgtgggcg taactttcta aaaaaaccag gtttaaatcg tcttttaatt ggagatggtt
    22501 tagcaaccgc attttctgct cttataggag ggccagctga aactacttat ggtgaaaata
    22561 caggtgttat tggcatgacg cgtatagctt ctgtaacggt tattcgtaat gcagctttta
    22621 ttgcaatcgc tttttcattc tttggtaaat ttacagcttt gatctctaca attcctagtg
    22681 cagtacttgg tggaatggct atattacttt atggtgttat tgcaagtaat ggattaaaag
    22741 tgttgattga aaatcgtgtc aattttgctg aggttcgtaa tctgattatt gccagttcca
    22801 tgttagtttt aggcctcggt ggagcagtcc ttgatctagg tgctctaact ttatcaggaa
    22861 cagcattaag tgctatagtg ggtattatat taaatttaat attgccaaaa gaaagcatga
    22921 aatcataaca aaagagctca gagtatctga gctcttttgt tatgatagat cagttaaaaa
    22981 ctttaaaata aaaacgttta cagttatggt ttccttattt tctgctataa tggtcataga
    23041 aaaagtgagg ttatagtaga atgaaattca atttagaaca agtaaaagta tttgagaatt
    23101 acgaaattga aaactgggaa gaaggtcaag ttaccttaac aacgaaggtc gttgactctt
    23161 cgctcaacta ttatggtaat gctcatggtg gttatttgtt tacactgtgt gatcaagtca
    23221 gtggtttagt tgctatttca acaggatatg aagcggtaac tctacaatca aatatcaact
    23281 atttgagggc aggccgatta gatgatctgc ttactgtcat cggtacttgt gttcataatg
    23341 gtcgtactac aaaagtcgtt gatgttcaca ttaaaaatca gaaagacgaa atggttacaa
    23401 ctgctatttt tacaatgttt gtaactggag agagaaaaga gaagtaaaaa aggtaggagt
    23461 tagacagcgt ctgtttaact cctttttcta ttgggaaaaa gatgaaaaaa tgatatacta
    23521 gagaggcaat tttgacaaaa ttaggagtag aaatgaatcc tttaataatt ggaatgaatg
    23581 ataaacaagc agaagcggta caaacgacag acggaccgct tttgattatg gcaggagctg
    23641 gctctggaaa aacacgtgtt ctgactcatc gtattgctta tttaatagat gaaaaatatg
    23701 ttaatccttg gaatatttta gcgattactt ttactaataa agcagcgcgt gaaatgcgtg
    23761 aacgtgctat cgccttgaat ccagctaccc aagatacttt gattgcaact tttcatagta
    23821 tgtgtgttcg tattttacgt cgtgaagctg attatattgg atataatcgt aactttacaa
    23881 tcgttgatcc aggtgagcag cgtactttaa tgaagcgaat cattaagcaa ttaaatttgg
    23941 acacaaaaaa gtggaatgaa cgttcaattt taggtacaat ctctaatgct aaaaatgacc
    24001 ttcttgatga aattgcatat gagaagcaag ccggagatat gtatacacag gtaattgcta
    24061 aatgctataa agcttatcaa gaggaattac gtagaagtga ggccatggat ttcgatgact
    24121 tgattatgat gacacttcga ttatttgatc agaataaaga tgttttggcc tattaccaac
    24181 agaggtatca atatatccat gtagacgagt atcaagatac taaccacgct caataccaat
    24241 tagttaagtt attagcttcg cgtttcaaaa atatttgtgt tgttggtgat gccgatcaat
    24301 ccatttatgg atggcgtgga gctgatatgc aaaatattct tgattttgaa aaggactatc
    24361 cgcaagccaa agttgtatta ttagaagaaa attatcgatc aactaagaaa atacttcaag
    24421 ctgctaataa tgtgatcaat cataataaaa atcgccgtcc taaaaaatta tggactcaaa
    24481 atgatgaagg tgagcaaatt gtatatcata gagctaacaa cgagcaagaa gaagccgttt
    24541 ttgtagcgtc aactattgat aacatcgttc gagaacaagg aaaaaatttc aaagattttg
    24601 ccgttcttta tcgtacgaat gcgcaatctc gtactattga ggaagcactt ttaaaatcca
    24661 atattccata tacaatggtt ggtggaacaa aattctatag tcgtaaagaa attcgagatg
    24721 ttattgctta tctcaatatc cttgcaaata cttctgataa tatttctttt gagcggattg
    24781 taaatgaacc taaaagaggg gtggggccag gaactttaga aaaaatacgg tcatttgcct
    24841 atgaacagaa catgtctctt cttgattcct cttcaaatgt catgatatcg cccttaaaag
    24901 ggaaggctgc tcaagctgtt tgggatttag ctaatctgat tttgactcta cgtagtaagc
    24961 tagatagttt aactgtaacg gagattacag aaaacctact ggataagaca ggatatctag
    25021 aagcacttca agttcaaaat acattagaaa gtcaagcgcg tattgaaaat attgaagaat
    25081 tcttatcagt gactaaaaat tttgacgata acccagagat tacagtagag ggtgaaactg
    25141 gtttagatcg tctatcgcgt tttttaaatg atttagcttt aatagcagat actgatgata
    25201 gcgccactga aacggctgaa gtcactttaa tgacattaca tgctgccaag ggtctagaat
    25261 ttccagttgt tttcttaatc ggtatggaag aaggagtatt tcctttatcg cgtgcaatag
    25321 aggatgctga tgaattagaa gaagagcgtc gtttagctta tgtcgggatt acgagagcag
    25381 aacaaatact tttccttacc aatgctaata cccgtacttt atttggtaaa actagttata
    25441 atagaccaac ccgttttata cgtgagattg atgatgagct gatacaacat caagggttgg
    25501 ctcgaccagt taattcttca tttggtgtga aatattcaaa agaacagcct acacaatttg
    25561 gtcaaggaat gagtcttcaa caagcgcttc aggcacgtaa aagtaattca caacctcaag
    25621 taactgccca actccaggct cttaacgcga acaatagcca tgagacatca tgggagattg
    25681 gtgatgttgc aactcataaa aaatgggggg acggtacggt gcttgaagtt tcaggtagcg
    25741 gaaagactca agagttaaaa ataaattttc caggtattgg gttaaagaaa ttattagcga
    25801 gtgtcgctcc tattagtaaa aaggaaaact aactttcttc tattatatgc ttaaataatc
    25861 agagactaag aattgtattc ttagtctttt tgcatcattt gaatagacaa aataagttat
    25921 catttggttt ttacttacta aaaaggttga tattaaagcg ttttccaaaa gtgaccatac
    25981 tattttaaag aaagaagcgt ttctcttgca ttcttttcta aaaagagtat aataacaata
    26041 tcaattaaat atcaagtggt cacttaaaaa taaaagcatt taccaagctt tttacttggc
    26101 agtcacttta taaagttgta taatattagt atagttaaca acttaattaa ttgccgagtg
    26161 gtcactttag aaactcttgt tatttagtta agaacaaaat aagtttgtac actgagatgt
    26221 acttaggaag aaaggtattt atcatggggt taatttggtc attaatcgtt ggtgctatta
    26281 ttggtgctat tgcaggagca atcactaaca aaggtggttc aatgggttgg atagctaaca
    26341 tcttagctgg tttagttggt tcatttgttg gtcaaagttt actaggaaca tgggggccta
    26401 agttagcaga tatggctttg attccttcaa tcgtaggtgc gattatcgtt atcattgtta
    26461 cttcatttgt attaggcaaa aaatcataac tacataacta gtagaatgat tagtcaagaa
    26521 agagaaggta tttatcatga gtttaatttg gtcattaatc gtaggtgcta ttatcggtgc
    26581 tattgcaggg gctgttacta acaaaggtgg ttcaatgggt tggatagcta acatcttagc
    26641 tggtttagtt ggttcatttg ttggtcaaag tttactagga acatgggggc ctaaattagc
    26701 aggtatggct ttgattcctt caatcgtagg tgcgattatt gttgtcattg ttacttcatt
    26761 tgtactaggt aaaatgaata actagaaaag gagttaccta tgtcaaaagg actaaaaagt
    26821 ttatatactt tgctaggtct aatctccctt accctactag gatttgtagc tgttatcagt
    26881 aaacaacaca tttatctacc atcttttaat tggttagatt gggattttaa tttgccatca
    26941 ccgattgacg ttggtatgta tcattatttc ttttggggag cattagtgct gtttgtaatc
    27001 gttttactgg ctatattggt agttctgttt tatccaagac gatacacaga gtataaactg
    27061 gctgataaaa ctggtaaatt gatgttaaaa aaatctgcaa tagagggatt tgttaaaaca
    27121 gaagtattaa aaactggttt gatgaaatct cctagtgtga cagctcattt atataagaaa
    27181 aaagtgaaag ttgatgttaa aggtctttta acttctcgta caaatgtacc ggaacaactt
    27241 gaacatattc aaagtgatgt tgaacttggc ttaaaagaat tctttgggtt ggaaaagaaa
    27301 atgaacacta gagtatttgt taagcaagtt gaagaagaaa atgttggaaa tgctaagacg
    27361 aataaaagtc gtgtagaata ggagtctaat atgtcagaat tcgtgaggaa atacagatat
    27421 ccactaggag gtgcagttat tgggctcgtt ttagctgcaa tgattgtaac cattggattc
    27481 tttaaaacaa ttcttgctct tgttatcatt gtgctaggtg cttatgctgg gctttatgtg
    27541 caaagaacag gtatgcttga ccaattcttt aataagagaa aataaaattt ttcgtttata
    27601 tttattctac attatttaaa taaaagaaag aggaactatt atgactgaaa cttacatcaa
    27661 aaacacaact aacaattctg gcacgacagc tgttcgtgga gaattaactt tcgaagataa
    27721 agtgattgaa aaaattgttg gaattgcgat tgagcatgta gatggtcttt tggctgtaaa
    27781 tggtggtttt ttctcaaatt taaaaaatag cgttgtaaat tctgattcag ttacagatgg
    27841 cgtaaatgtt gaagtgggta aaaagcaagt tgctgttgat ttggatattg ttgctgaata
    27901 ccaaaaacat gttccaacta tctttgccga tatcaaaaaa gttgttgaag ctgaagtgaa
    27961 acgtatgaca gaccttgaag tggttgaagt taatgttaat gttgttgata ttaaaactcg
    28021 tgctcaacat gaagaagatt cagtaactct tcaagatcgt gtaacaagtg cagcacaagc
    28081 aacaggtgaa tttgcttcga atcaagtttc aaacgttaaa agtgccgtag gtaacggtgt
    28141 tgataaagtg gaagacatga agtctgaacc acgtgtacaa taattgattt aagtatagtt
    28201 taaagtattg ttattagtag gaggctatta tgtctgaaga aaaatttgat gcaaaagttg
    28261 ataaggtttc tggttcagta aaagaatctg ttgggaaact tactggcgat aaagaagttg
    28321 aatctgaagg taaagtcgat aaattgaaag gtcatgcaaa agaaaaactt gctgacatta
    28381 aagataccat taagggtgct tcagagagct ttaagaaaaa ggactagaag gtttgaggtc
    28441 atttatttat ggaaaatcaa aaacaagata ttaaaacttc agtaacttat gaagaaaagg
    28501 taattgctaa aattgttgga catgctttag aatcagtaga cggactcttg gctgtgagtg
    28561 gaggtttctt ctctaatcta aaaaatagcg ttgtaaattc tgactcagta actgatggtg
    28621 taaatgttga agttggtact aaagaagttg cagttgattt agacatcgtt gttgaatacg
    28681 gcaaggatat cccagctatc gttgaaagca ttaaagcgat tgtcagtcaa aacgttgaag
    28741 tgatgaccca ccttaaagtt gttgaattga acgcaaatgt cgtagatgtt aagactaaag
    28801 ctgaacatga agctgactca gtgacagtac aagatcgtgt atctgatgca gcacaagcaa
    28861 caggaaactt tgcttctgaa caagctggca aagctaaagc agcaatttct tctggagcag
    28921 agaaaacaaa agaagcagta tcaaatggta ctgaggctgc aaaagaaaaa atctcagaag
    28981 ctcgtactag tgaatcttaa ttcagtaaac tactttttat gataagacac tcggagctgg
    29041 gagaccagct ccttttatat gatatttact aaagacatta gatattaaca aaaaagatat
    29101 atttgcaata ttttgctaat tgtggtttaa tatgtatgtt tttattagga taaggagggt
    29161 tggatggttt ctgaacaatc tgaaattgta acatctaaat atcaaaaaat agcagtagcg
    29221 gttgcacaaa gaatcgcaaa tggagattat gaagttggag agaagttgaa atctagaaca
    29281 actattgctt caacattcaa cgtatcacca gaaacagcac gtaagggtct taatatttta
    29341 gcagatttac agattttaac tttaaagcat ggcagtggag ccattattct ttctaaagaa
    29401 aaagcaattg cattcttaaa ccaatatgaa acctctcatt cagttgctat tttgaaaggt
    29461 aaaatccgtg ataatattaa ggctcagcaa caagaaatgg aagagttagc tactttagtt
    29521 gatgattttt tgcttcaaac acgtgcggtt agtaaacaat acccactagc accatatgaa
    29581 attattgtca gtgaagattc tgaacacctt ggtaagtcaa taggagaatt aaatgtatgg
    29641 catcaaactg gtgcgactat tgtggctatt gaacacgaag ggaaatttat tgtttctcca
    29701 gggcctttct cagttataga gcaaggagat catatctttt ttgttggtga tgaagatgtt
    29761 tatgcacgta tgaaaaccta ttttaactta aggatggggc tttgagagaa tggattttgt
    29821 ctttgatata gatggaactc tttgttttga tggtatgagt ctatctaaag aaatacaagg
    29881 aatactagag agagcccaga ttgattatgg tcatagagtc acttttgcta cggcgcgatc
    29941 ttatagagac actataggta tccttggcga caagctatct ttatcaaaaa ttattggttt
    30001 aaatggtgcg actttacatg aaaatggtca cttagtggat agttactatt tacaaagtga
    30061 cttttttagt actattattt catattgtca tcgctatcag attccatatt ttgttgatga
    30121 ggtctttaat tacgctactt atcaggcttc taaaattccc tttattgctt atgttgatcc
    30181 tcaaaaaagg ggagagcttc tagaagtttc taaaattgag aaaccgataa aaatggtgtt
    30241 gtattttggt gatcagctga gaagggcaga ccaaatgtta gcgaaattaa atcgttttgg
    30301 acttagtagt cactttttcc acgaatttga aaaatgtttg tacataaatc ccattgcagt
    30361 tgacaaggga aaagccacta agaaattatt tgggaatcgt tttatagctt ttggcaatga
    30421 taaaaatgat atttctatgt ttgatgctgc gcattatagt gttcaagtag gggattttga
    30481 tgaactgact ccttacgcta atttacgtgt atcaagagaa tctgtacatg aaggaattac
    30541 aatgcttttt gaaaaataca aggggattac gtaatggatt tattcctaag tttagagagg
    30601 aaatttaaag ctgcaagtga caaagaagtt tctaagcaac aggaggctta tttacgccat
    30661 cattttaagt gctatggtat taaatcacct gagcgacgta tgctctacaa agagcttata
    30721 aaggccgcta aaagacaagc aaaaattgat tggcaattgc ttgataaatg ttggcaaagt
    30781 gactatcgtg aatatcatca ttttgtactt gattatctat tagctatgag tcaatttctc
    30841 acctataatg attgctctag actagagttt tacgctcgtc atcaacagtg gtgggatagt
    30901 attgatgttc tgactaagat ttttgggaac ttatccttaa aagatgacaa ggttatgaat
    30961 ttactgtcag aatggtcact tgatcaagat ttttggatga ggcgactagc tattgagcat
    31021 caactaggat ttaaagaaaa aacaaatact gatattttgt cactttttat tttaagaaat
    31081 acaggaagtc aagaattttt tatcaataag gcgattggct gggcgcttag ggattattca
    31141 aagtataata aagtatgggt aaaggacttt atcagtaatc attatgatga gttgtcgact
    31201 ttgagcatac gcgaaggaag taagtacctc taattatcag ttcaattatt cgaaaaagaa
    31261 gaagcttcat agtaaaataa aggtaattga taaggagata aacttatgac gacatttctt
    31321 ggaaatcctg ttacttttac aggaaaacag ctacaagttg gggatattgc aaaagatttt
    31381 ttactaatag ctacagactt atctcaaaaa tctttaaaag attttgaagg gaaaaagaaa
    31441 gttattagcg ttgttccttc tattgataca ggtatttgtt caaaacaaac acgtactttt
    31501 aatgaggaat tatcagagct agataacaca gtggttatta ctgtttctat ggaccttcca
    31561 tttgctcaga agcgttggtg cagcgctaaa ggtcttgata acgtgatttt attatcagat
    31621 ttttatgatc attcgtttgg tcaagagtac gctcttttga tgaatgagtg gcatttgtta
    31681 acgcgtgcag ttcttatttt ggatgagcat aataaggtga cttacaccga atacgttgac
    31741 aatgttaatt cagatgttga ctacgaagcg gcgattaatg ctgcaaagat attaccataa
    31801 ataataaggc tggaaaattt tccagcctta ttatttatat agacagtgag aacaattaga
    31861 gatgaaatgt tgtcattatt catctttttc aatatcaaca ttgtttttaa atattagata
    31921 cataactcca ttgtaagcta caccggacat aatactagac aaaataactt ttaaagaaaa
    31981 tagtgataca atcagcggag tgttattgtt agttgttaaa cccaagataa gccactggat
    32041 gacagtataa aatatcccga ttttgataaa tttttgtttg tttttcttta ttgctactgg
    32101 atagtcattt tttgagactt ctagtggcgt tattaaatca tgactgttga gataagaggg
    32161 aataacaatt agaaaaatac aaaaatatag aaagaatgtt aaagctagga gagtacatgc
    32221 agtctctgtg ggatatttac ttaccgtgaa ccaggcgatg atagaggata aaatgtgata
    32281 gagactagtg gttgcaaata atagatttcc aaggtaattg attttttgtt ctctgtattc
    32341 atcgaatggg ccattaatac catataggga ttttattaat tttatcccca aatttttctt
    32401 ttctttcata atattctcct atccttgaac tatttaataa agtttgagat tgaaataata
    32461 atacgaccta acctcccgag ttttcataga tttagagagt ctaatctgtt atagatgtct
    32521 actatcccaa aaaagagtat tgaggtctgt ttgtaggcat tccgctagtc ttatacaaag
    32581 atctagcgaa ggattgtatt tattattttc aataaggttt attgtttgtc tggcgacacc
    32641 aactgatttt gctaaagcta attgagatat gctagcttta attctgtatt ctctaagacg
    32701 attcacgatt attagctcct ttagtaaaga a
//
LOCUS       DATRUK010000018        30684 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.018, whole genome shotgun sequence.
ACCESSION   DATRUK010000018 DATRUK010000000
VERSION     DATRUK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30684
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.018"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(353..1162)
                     /locus_tag="VBE34_000791"
     CDS             complement(353..1162)
                     /locus_tag="VBE34_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco47II family restriction endonuclease"
                     /protein_id="HEO7263521.1"
                     /translation="MWGLSFISEEDFYNHVQLTIDKYGKKLQSFDLKRFNKNIVDPIK
                     LIFDKSVYRSSWEQIISNEIFRQRDKSNNNDIGYFHQTIFQYIEHCRVPNNGEEGGWD
                     VIFENPNGITMPDGSQVSRVYVEMKNKHNTMNSASSGKTFMKMQNQLLQDDDCACFLV
                     EAIAKNSQNIQWEPKVDGRKMGHKYIRRVSLDQFYSLVTGQEDAFYNMCMVLPEVINK
                     AVSELDSSIIPNDTVFDEIRDISAQQNYDSEDLSIAMSFYLLGFSSYLGFE"
     gene            complement(1163..2164)
                     /locus_tag="VBE34_000792"
     CDS             complement(1163..2164)
                     /locus_tag="VBE34_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEO7263522.1"
                     /translation="MKSIELFAGAGGLALGIEKAGFDTIGLVEFDSAASETLKYNRPN
                     WNVIHDDVANISKLDLEEYFLIKKGELDLLSGGAPCQSFSYAGKRLGLEDTRGTLFYH
                     YAVFLEKLQPKMFLFENVKGLTSHDKGKTYKTILNVFEEEGYTVQSKVLNAWDYGVAQ
                     KRERMIMVGIRNDLVNMTSFEYPKPHDYKPVLRDILLDCPPSQGMQYSEQKRKIFELV
                     PPGGYWKDIPESIAKEYMKSTWYMGGGRTGILRRLSLDEPSLTVLTSPTQKQTERCHP
                     LEVRPFTIRENARIQSFPDDWEFQGSVGNQYKQVGNAVPVNLAYEVGLKIKESLEVL"
     gene            complement(2459..2824)
                     /gene="rplL"
                     /locus_tag="VBE34_000793"
     CDS             complement(2459..2824)
                     /gene="rplL"
                     /locus_tag="VBE34_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEO7263523.1"
                     /translation="MALNIENIIAEIKEATILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGEAAAAKDSFDVELTAAGDKKVGVIKVVREITGEGLKEAKAIVDNAPSVIKEGASEA
                     EANEIKEKLEAAGASVTLK"
     gene            complement(2888..3388)
                     /locus_tag="VBE34_000794"
     CDS             complement(2888..3388)
                     /locus_tag="VBE34_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEO7263524.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVEQDTNLRR
                     SLRESDVEFKVIKNSILTRAAEKAGLEDLKELFVGPSAVAFSNEDVIAPAKVISDFAK
                     DAEALEIKGGSVDGKFTSVEEINALAKLPNKEGMLSMLLSVLQAPVRNIAYAVKAVAE
                     KDEEVA"
     misc_feature    complement(3418..3551)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            3821..5929
                     /locus_tag="VBE34_000795"
     CDS             3821..5929
                     /locus_tag="VBE34_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO7263525.1"
                     /translation="MANNNFYNRDPFGNMDDIFNSLMGNMGGYNSENKRYLINGREVT
                     PEEFSQYRQTGKLPGQELNNQNTPTNQVSADSVLTKLGTNLTDQARQHLLDPVIGRNK
                     EIQETAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIINGDVPAAIKNKEIISIDIS
                     SLEAGTQYRGSFEENIQNIIKEVKETGNIILFFDEIHQILGAGSTGGDSGSKGLADIL
                     KPALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAQDTFNILMGIRNLY
                     EQHHNVVLPDSVLKAAVDLSIQYIPQRSLPDKAIDLIDMTAAHLAAQHPVTDLKSLEK
                     EIAEQRDKQEKAVNTEDFEEALKVKTRIEELQNQIDNHTEGQKVTATINDIAMSIERL
                     TGVPVSNMGASDIERLKELGNRLKGKVIGQNDAVEAVARAIRRNRAGFDDGNRPIGSF
                     LFVGPTGVGKTELAKQLAFDMFGSKDAIVRLDMSEYNDRTAVSKLIGATAGYVGYDDN
                     SNTLTERIRRNPYSIVLLDEIEKADPQVITLLLQVLDDGRLTDGQGNTINFKNTVIIA
                     TSNAGFGNEAFTGDSDKDLKIMERISPYFRPEFLNRFNGVIEFSHLSKDDLSEIVDLM
                     LDEVNQTIGKKGIDLVVDENVKSHLIELGYDEAMGVRPLRRVIEQEIRDRITDYYLDH
                     TDVKHLKANLQDGQIVISER"
     gene            complement(6022..6966)
                     /gene="mmuM"
                     /locus_tag="VBE34_000796"
     CDS             complement(6022..6966)
                     /gene="mmuM"
                     /locus_tag="VBE34_000796"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HEO7263526.1"
                     /translation="MGRFKELLESKKTLILHGALGTELESRGCDVSGKLWSAKYLIED
                     PAAIQTIHEDYIRAGADIVTTSTYQATLQGLAQVGVSESQAEDLIRLTVQLAKAAREQ
                     VWKSLTKEEKSERIYPLISGDVGPYAAFLADGSEYTGLYDIDKQGLKNFHRHRIELLL
                     DEGVDILALETIPNAQEAEALIELLAEDFPQVEAYMSFTSQDGKTISDGSAVADLAKA
                     IDVSPQVVALGINCSSPSLVADFLQAIAEQTNKPLVTYPNSGEVYDGASQSWQSSPDH
                     SHTLLENTSDWQKLGAQVVGGCCRTRPADIADLSAHLK"
     gene            complement(6971..8347)
                     /locus_tag="VBE34_000797"
     CDS             complement(6971..8347)
                     /locus_tag="VBE34_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO7263527.1"
                     /translation="MENHRYEDEGKFQRKMTSRHLFMLSLGGVIGTGLFLSSGYTIAQ
                     AGPLGAVLSYLIGAVVVYLVMLSLGELAVAMPVTGSFHTYATKFISPGTGFTVAWLYW
                     ICWTVALGTEFLGAAMLMQRWFPNVPAWAFASFFALVIFGLNALSVRFFAEAESFFSS
                     IKVIAIIIFIILGLGAMFGLVSFEGQHKAILFTHLTANGAFPNGIVAVVSVMLAVNYA
                     FSGTELIGIAAGETDNPKEAVPRAIKTTIGRLVVFFVLTIVVLASLLPMKEAGVSTAP
                     FVDVFDKMGIPFTADIMNFVILTAILSAGNSGLYASSRMLWSLANEGMLSKSVVKINK
                     HGVPMRALLLSMAGAVLSLFSSIYAADTVYLALVSIAGFAVVVVWLAIPVAQINFRKE
                     FLKENQLEDLSYKTPFTPVLPYITIILLLISIVGIAWDSSQRAGLYFGVPFIIFCYIY
                     HKLRYKKW"
     gene            complement(8477..9127)
                     /locus_tag="VBE34_000798"
     CDS             complement(8477..9127)
                     /locus_tag="VBE34_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HEO7263528.1"
                     /translation="MSYFRNYWYRFGAILFIILAVILLVFRPDWSMLHYLLYFYFMAL
                     LAHQFEEYQFPGGASPIINYVVYDEEELMDCFPGNTQSIMLVNTIAWLLYIASIAFPQ
                     AYWLGLGVMFFSLTQLLGHGFQMNIKLKTWYNPGLATTVFLLVPIACAYIYQASAEGM
                     LTWGDWLGGFIMLIVCVLTSIIAPVQLLKDKETNYIISPWQMDRFHKVVNFVRIKK"
     gene            complement(9139..9788)
                     /locus_tag="VBE34_000799"
                     /pseudo
     CDS             complement(9139..9788)
                     /locus_tag="VBE34_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
     gene            9880..10428
                     /locus_tag="VBE34_000800"
     CDS             9880..10428
                     /locus_tag="VBE34_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO7263529.1"
                     /translation="MTESVTVNKKRIKQITQTQEDILTAFGQLLEEYPYEKIQISQIA
                     KKSGYARRTFYRHFDSRDDLLTLFIERLTLNLFKQLGQLEQPTFSQVFQNFFSYWSDY
                     KSLLLILRKNDLLPQFQQSWFRHIDLIELGRGDLSSNTYAQRFAIGGIFSVLIEWIHQ
                     DCQTSIEELTQVSFDIINHLKN"
     gene            complement(10490..11086)
                     /locus_tag="VBE34_000801"
     CDS             complement(10490..11086)
                     /locus_tag="VBE34_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEO7263530.1"
                     /translation="MAEEFLNTHNASILLSAANKSHYPQDDLPEVALAGRSNVGKSSF
                     INTLLGRKNLARTSSKPGKTQLLNFYNIDDKLRFVDVPGYGYAKVSKTERAKWGKMIE
                     EYLVTRDNLRVVVSLVDFRHDPSADDIQMYEFLKYYEIPVIIVATKADKIPRGKWNKH
                     ESSIKKKLNFDKKDHFIVFSSVDRTGLDESWDTILSEL"
     gene            complement(11097..12323)
                     /gene="clpX"
                     /locus_tag="VBE34_000802"
     CDS             complement(11097..12323)
                     /gene="clpX"
                     /locus_tag="VBE34_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEO7263531.1"
                     /translation="MAGNRNNDMNVYCSFCGKSQDEVKKIIAGNGVFICNECVALSQE
                     IIKEELAEEVLADLAEVPKPKELLEILNQYVVGQDRAKRALAVAVYNHYKRVSYTESS
                     DDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENIL
                     LKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVA
                     SVPPQGGRKHPNQEMIQINTKNILFIVGGAFDGIEDLVKQRLGEKVIGFGQTSRKIDD
                     NASYMQEIISEDIQKFGLIPEFIGRLPVVAALELLTAEDLVRILTEPRNALVKQYQTL
                     LSYDGVELEFDQDALLAIADKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVRI
                     TKAAVEGTDKPVLETA"
     gene            complement(12541..13035)
                     /locus_tag="VBE34_000803"
     CDS             complement(12541..13035)
                     /locus_tag="VBE34_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO7263532.1"
                     /translation="MTKQIIAIWAEDEDHLIGVNGGLPWRLPKELHHFKETTMGQALL
                     MGRKTFDGMNRRVLPGRETIILTKDEQFQADGVTVLNSVEQVIKWFQEHNKTLFIVGG
                     ASIYKAFLPYCEAIIKTKVHGKFKGDTYFPDVNLSEFKVISRDYFEKDEQNAHAFTVT
                     YFEK"
     gene            complement(13115..13954)
                     /locus_tag="VBE34_000804"
     CDS             complement(13115..13954)
                     /locus_tag="VBE34_000804"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEO7263533.1"
                     /translation="MTKADLLFKDNITKIMSEGVFSEQARPRYKNGEMANSKYITGAF
                     AEYDLSKGEFPITTLRPIPIKSAIKEIFWIYQDQTNDLAVLNDKYGVTYWNDWEVGHT
                     GTIGQRYGAVVKKHNIISKLLKQLEDNPWNRRNVISLWDYEAFEETEGLLPCAFQTMF
                     DVRRVNGELYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWRVGKFFYFINNL
                     HIYDNQFEQAQELLKRQPSECNPKLVLNVPDGTDFFDIKPDDFALVDYDPIKPQLRFD
                     LAI"
     gene            14003..15280
                     /locus_tag="VBE34_000805"
     CDS             14003..15280
                     /locus_tag="VBE34_000805"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEO7263534.1"
                     /translation="MRNKTLKVKAFFHIFSFFMVKWNLAKMTFIQKGLPMKIGIDKIG
                     FATSQYVLEMTDLAIARQVDPEKFSKGLLLDSLSITPVTEDIVTLAASAANDILSDED
                     KETIDMVIVATESSIDQSKAASVYVHQLLEIQPFARSFEMKEACYSATAALDYAKLHV
                     EKHPDSKVLVIASDIAKYGIKSTGESTQGAGSIAMLISQNPSILELKEDHLAQTRDIM
                     DFWRPNYSDVPYVNGMFSTKQYLDMLKTTWKVYQKRFNTSLSDYAAFCFHIPFPKLAL
                     KGFNKILDNNLDEQKKAELQENFEHSITYSKKIGNCYTGSLYLGLLSLLENSQNLKAG
                     DQIAFFSYGSGAVAEIFTGQLVDGYQNKLQSDRMDQLNKRQKITVTEYEKLFFEKTIL
                     DENGNANFNTYRTGTFSLDSICEHQRIYKKINN"
     gene            15282..16565
                     /locus_tag="VBE34_000806"
     CDS             15282..16565
                     /locus_tag="VBE34_000806"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEO7263535.1"
                     /translation="MTKISWTGFSKKSPEERIHYLEEQDFLADSSLEIVTNQDLLSLS
                     LANQMAENVIGRIALPFSLVPDVLVNGKVYQVPYVTEEPSVVAAASFAAKIIKRSGGF
                     LTTVHNRKMIGQVALYDVQDSQHTKESILNQKQQLLEIANAAHPSIVKRGGGACDLTI
                     EIKEDFLIVYLMVDTKEAMGANMVNTMMEALSSPLEDISKGKSLMSILSNYATESLVT
                     ATCRVDLRFLSRQKEEAIKLAQKMTMASQLAQVDPYRASTHNKGIFNGIDAIVLATGN
                     DWRAIEAGAHTYAVKDGQYRGLSRWSYKVDDNCLEGTLTLPMPVATKGGSIGINPSVH
                     LAHDLLGRPNAKELASIILSIGLAQNFAALKALVSTGIQAGHMKLQAKSLALLAGAKE
                     EQISEVVKQLLDSKHMNLETAQKIVNKLTKSKKRI"
     gene            16657..17106
                     /locus_tag="VBE34_000807"
     CDS             16657..17106
                     /locus_tag="VBE34_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEO7263536.1"
                     /translation="MNKNQYPKWEELPELDLYLDQVLLYVNQLINPKTITNDKLLTAS
                     MINNYVKHNYISKPIKKKYNRRQVARLIVITAFKQIFAIQEISQTLELLTADNHSEEA
                     YNGFAACMNKEEVHDLPPVVISACQTLNLYQETQKLVLELGGKNKNA"
     gene            17099..17755
                     /locus_tag="VBE34_000808"
     CDS             17099..17755
                     /locus_tag="VBE34_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEO7263537.1"
                     /translation="MLKEMSIKLSPQLSFGEELANSITHAVGALLMLILLPITAVYSH
                     NHFGLQAALGTSIFVTSLFLMFLSSSIYHSMTYNSLQKYVLRMIDHSMIYIAIAGSYT
                     PVALSLIGGWLGYLIIFLQWGITLFGILYKIFAPKINDKFSLVLYLIMGWLVIFIFPA
                     IITKTGPAFWGLLLAGGICYTIGALFYARKRPYDHMIWHLFILLASILQYIGIVYFML
                     "
     gene            17842..18756
                     /locus_tag="VBE34_000809"
     CDS             17842..18756
                     /locus_tag="VBE34_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO7263538.1"
                     /translation="MKSAYIFFNPKSGKDEQALAKEVKSYLIEHDFQDDYVRIITPSS
                     VEEAVALAKKASEDHIDLVIPLGGDGTINKICGGVYAGGAYPTIGLVPAGTVNNFSKA
                     LNIPQERNLALENLLNGHVKSVDICKVNDDYMISSLTLGLLADIAANVTSEMKRKLGP
                     FAFLGDAYRILKRNRSYSITLAYDNNVRSLRTRLLLITMTNSIAGMPAFSPEATIDDG
                     LFRVYTMEHIHFFKLLLHLRQFRKGDFSQAKEIKHFHTNNLTISTFKRKKSAIPKVRI
                     DGDPGDQLPVKVEVIPKALKFIIPNSLP"
     gene            18857..19711
                     /locus_tag="VBE34_000810"
     CDS             18857..19711
                     /locus_tag="VBE34_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO7263539.1"
                     /translation="MTTFTAKFIDQEWEVPVESGRYHMIVGEFCPYAQRPQIARQLLG
                     LDKHISISFVDDVPSDIGLIFSQPEQVTGAKSLRDIYHLTDPTYQGPYTIPILIDKTD
                     NRIVCKESADLLRLFTTDFSDLHQEDAPVLFSQETASLIDNDIKDINKNFQSLMYKLA
                     FLDKQADYDTYSKEFFTFLDQKEHLLGQRPFLLGDNLSEVDIHFFTPLVRWDIAGRDL
                     LLLNQKALEDYPNIFSWAKTLYNDFNLKTLTNPQSIKNNYYLGKFGRAVRHHTIVPTG
                     PNMAKWEK"
     gene            complement(19757..19975)
                     /locus_tag="VBE34_000811"
     CDS             complement(19757..19975)
                     /locus_tag="VBE34_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEO7263540.1"
                     /translation="MIKLYYKDAYDTERCQVFENPNDFIRVLMGCVTVPDNYPITKMT
                     YKDKDIAYSGTYGDLFKTFIEFDWSTLD"
     gene            complement(20043..21038)
                     /locus_tag="VBE34_000812"
     CDS             complement(20043..21038)
                     /locus_tag="VBE34_000812"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEO7263541.1"
                     /translation="MTNRKDDHIKYALKYQSHYNSFDDIELIHSSLPKYNVNDIDLST
                     HFAGQSFEFPFYINAMTGGSEKGKAVNHKLAQVAQATGIVMVTGSYSAALKNDEDDSY
                     PTTDLYPDLKLATNIGLDKPVPAAESTVKAMNPIFLQVHVNVMQELLMPEGEREFHMW
                     RSHLKEYVDNIQCPLILKEVGFGMDLQSIKDAYDIGITTVDISGRGGTSFAYIENQRG
                     RDRSYLNTWGQTTAQSLINAQSMMDKMDILASGGIRHPLDMVKCLVLGAKAVGLSRTV
                     LELVERYPVDDVIAILNSWKEDLRMIMCALNCKKITDLRQVNYILYGQLKEANAK"
     gene            complement(21035..22027)
                     /locus_tag="VBE34_000813"
     CDS             complement(21035..22027)
                     /locus_tag="VBE34_000813"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEO7263542.1"
                     /translation="MVKVQTGGKLYIAGEYAILYPGQVAILKNVPIYMTALATFADNY
                     SLYSDMFNYTASLQPDKQYSLIQETILLMEEWLINFGKNIKPIHLEITGKLERYGLKF
                     GIGSSGSVVVLTIKAMAALYEIEMPSDLLFKLSAYVLLKRGDNGSMGDIACIAYEHLI
                     SYSAFDRRAVSKMIETKPLEQVLEAEWGYRITKIQALLEMDFLVGWTMQPSISKEMIN
                     IVKSTITQRFLDDTKYQVVQLLSAFKEGDKEAIKRCLEEISLLLFNLHPSIYTDKLQK
                     LKEASKGLDIVTKSSGSGGGDCGIAISFNKNDNQTLIKRWESAGIELLSKETLS"
     gene            complement(22020..22964)
                     /gene="mvaD"
                     /locus_tag="VBE34_000814"
     CDS             complement(22020..22964)
                     /gene="mvaD"
                     /locus_tag="VBE34_000814"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEO7263543.1"
                     /translation="MDGKSISVKSYANIAIIKYWGKADAEKMIPATSSISLTLENMYT
                     ETRLTALGKDAKKDEFYISGVLQNDHEHDKMSAILDRFRQNRSGFVKIETTNNMPTAA
                     GLSSSSSGLSALVKACNDFFGTNLSQSQLAQEAKFASGSSSRSFFGPVAAWDKDSGDI
                     YKVHTNLDLAMIMLVLNDKRKPISSREGMKICTETSTTFNEWVRQSEQDYQDMLVYLK
                     NNDFQKVGQLTERNALAMHSTTKTATPAFSYLTEETYKAMDVVKKLREKGHECYYTMD
                     AGPNVKVLCLRQDLEALAAILEKDYRIIVSTTKELADG"
     gene            complement(22946..23824)
                     /gene="mvk"
                     /locus_tag="VBE34_000815"
     CDS             complement(22946..23824)
                     /gene="mvk"
                     /locus_tag="VBE34_000815"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEO7263544.1"
                     /translation="MKEKFGIGKAHSKIILMGEHSVVYGYPAIAIPLKNIEVTCLIEE
                     APQLIALDMTDPLSTAIFAALDYLGKTSSKIAYHIESQVPERRGMGSSAAVAIAAIRA
                     VFDYFDEDLEADLLECLVNRAEMIAHSNPSGLDAKTCLSENTIKFIRNIGFSTVPMHL
                     NAYLVIADTGIHGHTKEAVDKVKSSGEAVLPFLKELGYLAEASEDAIHKSDSKQLGSL
                     MTKAHQSLKQLGVSSLEADHLVEVAISCGALGAKMSGGGLGGCIIALVKEKREAERLS
                     QQLEREGAVNTWTEKV"
     gene            complement(23940..25169)
                     /locus_tag="VBE34_000816"
     CDS             complement(23940..25169)
                     /locus_tag="VBE34_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7263545.1"
                     /translation="MFRNLRLRFIGIAALAILVVLFSVVGVLNSANHYQTKNEIYRVL
                     TILADNNGRIPNKLEFSKELGDDLSTDAIFQFRYFSARTDAKGNVTSFDSRNIFEVSD
                     RQIKNYAKRIVSQNSHSGHITYNFSTYSYLLKKVGKNDYLVVFLDTTNQYLDNQRLLQ
                     LSIWMSLVSFIVFMVIVSVLSGRVILPFVANYEKQRRFITNAGHELKTPLAIISANNE
                     LVEMMSGESEWTKSTNDQIQRLTGLINGMVSLARFEEQPDISMVDLDFSHITKDAAED
                     FKGPIIKDGKDFIMSIQPGIHVKAEEKSLFELVTLLVDNANKYCDPMGTVTVKLSRSS
                     RLRRAKLEVSNTYKNGKDIDYSKFFERFYREDESHNNKKSGYGIGLSIVTSLVHLFKG
                     SIDVNYKHDTITFVIYI"
     gene            complement(25169..25855)
                     /locus_tag="VBE34_000817"
     CDS             complement(25169..25855)
                     /locus_tag="VBE34_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7263546.1"
                     /translation="MRVLIAEDEEQMSRVLSTAISHQGYVVDVAYDGQTAIDLANQNA
                     YDVMVMDVMMPVKTGVEAVKEIRQSGNKSHIIMLTAMAEIDDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMSRRLEDFTPNVLSLGRVTLSVGEQELQCENTIRLAGKEAKMLAFF
                     MLNHDKELSTQQLFEHVWGADKDQEDVDEGYIFIYVSYLRQKLQAIGANLEIIGQEGS
                     SYKLSEINGG"
     gene            complement(25857..26483)
                     /locus_tag="VBE34_000818"
     CDS             complement(25857..26483)
                     /locus_tag="VBE34_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO7263547.1"
                     /translation="METNIYGDYGRYLPLILEDFSQRIQLENDKAKVETGYKLYEHII
                     GRIKTSDSMIEKCRRKQLPVTVDSALKVIRDSIGVRIICGFVNDIYQIVERIKTFDDC
                     RIVVEKDYIQHVKPNGYRSYHVILEIDTPYPDCLGNSEGKYYMEIQLRTIAQDSWASL
                     EHQMKYKHDIENPERIVRELKRCADEIASVDLTMQTIRQLIESGTKKE"
     gene            complement(26591..28345)
                     /locus_tag="VBE34_000819"
     CDS             complement(26591..28345)
                     /locus_tag="VBE34_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7263548.1"
                     /translation="MKTKSQKTSFFSNLSPYLKGYKLLFALAIVLATLSSLTTVIGPD
                     RLKEMTDLMVKGLAGNIDLAKIGHIAGQLAVLYVLGALVSYLASFIVTTIIQKFAQGM
                     RNAIADKINKIPLSYFDSHTQGDTLSRVTNDVDLMTQSFNQSLVTMVASTVLLVGSLI
                     MMFKTDWHLATTAIISVFSGFALSSIIMVKSQPLFKKQQVNLANISGYVEEIYSGHNI
                     VTSHNARQQAKSDFQLLNDKLHSSMWKSQFFSGIMMPLMQFIGNFGYVMVCVVGAMLA
                     IKGTITMGTIVAFMTYVRIFTQPIGQIAQGITQLQSASAAMGRVFEFLNETNMQEDKH
                     KVLNLPKFKGHVSFEHVKFGYSDNKTIIHDFTSEAQPGQKVAIVGPTGAGKTTIVNLL
                     MRFYDVNSGSISIDDINIQDMKRSQVHDAFSMVLQDTWLFEGTIKENLIFNQTTISDD
                     EVQEAAKAVGIHHFIMTLPKGYDTVLDDAVTLSVGQKQLLTIARALLKNSPLLILDEA
                     TSSVDTRTEELIQKAMDKLMEGRTSFVIAHRLSTIKNADLILVMKDGNIIEQGNHDVL
                     MSQSGFYADLYNSQFESA"
     gene            complement(28335..30152)
                     /locus_tag="VBE34_000820"
     CDS             complement(28335..30152)
                     /locus_tag="VBE34_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7263549.1"
                     /translation="MFNIFKRLNGKEWLMIVASTILVCLAVWMDLKTPEYMSDITRML
                     QTKGTKLEALWDPGLKMLAFSFGSFLVAVMVGFLGARTAASFTSRLRSDIFNHVMDYS
                     DAEIKRFSIPSLLTRTTNDLTQLQILIVMGMQVVTRGPIMAIWALTKIGGKSDQWTGA
                     VGVAVIIVMILLSVLLLLAFPRQRQIQGFTDALNATTREALSGVRVIRAYNAEKYQTD
                     KFKRENDDLTKLNLFVYRLMSLMNPVMTVVSSGLTLAIYWIGSYLINDISVPKVTGTS
                     VSVLAAKSAITDRVDLFSNMVVFSSYAMQVVMGFMMMVAIFIIMPRALVSARRINEVL
                     TLAPTVTFKENSHIQSAPKGQIEFQDVSFHYSKDSEAVIEHVSFKADTGQTVAFIGST
                     GSGKSTLVNLVPRFYDATEGSIKLDGIDIKEYSHNDLNNKIGYIPQKAVLFSGTVRSN
                     MIFGESGREELSDEQIWQALELAQAKEFIEAKEKGLDTEVAQGGSNFSGGQKQRLAIA
                     RALARHPEILIFDDSFSALDYKTDHILRHVLKEKTAAMTKLIVAQRISTIMDADLILV
                     LDQGKVVGQGTHKELLSNNEVYQEIAYSQLSKEELENEN"
     gene            complement(30199..30639)
                     /locus_tag="VBE34_000821"
     CDS             complement(30199..30639)
                     /locus_tag="VBE34_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7263550.1"
                     /translation="MENPLQKARILVNQLEKYLDRYAKEYDVEHLAGPQGHLVMHLYK
                     HPDKDMSIKDAEEILHISKSVASNLVKRMEKNGFIAIVPSKTDKRVKYLYLTHLGKQK
                     ATQFEIFLEKLHSTMLAGITKEEIRTTKKVIRTLAKNMAMEDFD"
BASE COUNT         9973 a         6106 c         4978 g         9627 t
ORIGIN      
        1 cacaattctt aagattattc acaaatctgt tagatagtga aatggctttt atcaaatctc
       61 tacttgggtc agcccaaaaa tgtctccctc catttatact atcattcaat attcttaatg
      121 tttccactaa ctgatggtgt aaactagaac atagctgact caacctatca tcaaaacttt
      181 ctttgtaaag atgttcataa tcaatagcaa actcttttcc aaagtaacta gtataatctc
      241 tttcaatact cactatgcca ttcaatagtt ttgcattgtt atttacagaa atcaattcta
      301 tttttctccc actatttata ttgctacaat ataaacatcc attcaaatta tatcattcaa
      361 aacctaaata actagaaaaa cctagtaaat agaatgacat tgcaatagat aaatcttcag
      421 aatcatagtt ttgttgagca ctaatgtctc taatttcatc aaatactgta tcatttggaa
      481 taattgaaga atctaattct gatacagcct tattaatgac ttctggcaaa accatgcaca
      541 tattatagaa ggcatcttcc tgtcccgtca caagtgaata gaattggtct aaactaacac
      601 gtcttatata tttatgtccc atttttcgac catcaacttt tggctcccat tggatatttt
      661 gagaattttt tgcaattgcc tcaactaaaa aacacgcaca atcatcatct tgtaatagct
      721 gattttgcat tttcataaaa gttttaccag aagaagcaga attcattgtg ttatgcttat
      781 ttttcatttc cacatagaca cgactgactt gagaaccatc tggcattgtt ataccatttg
      841 gattttcaaa aatgacatcc caaccaccct cttctccatt attcggaact cgacagtgct
      901 ctatgtattg gaaaatagtt tgatgaaaat atccaatatc attattgttt gatttatcac
      961 gttgtctaaa aatctcgtta cttataattt gttcccacga acttctataa acagatttat
     1021 caaaaatcaa ttttattgga tcaacaatat ttttattaaa tcgtttcaaa tcaaaggatt
     1081 gtaatttttt gccatacttg tctatagtca attgaacatg attataaaaa tcttcttcac
     1141 taataaatga taagccccac atttataata cctccaaact ttcttttatc ttcaatccta
     1201 cttcataagc tagatttaca ggaacagcat ttccaacttg tttatattga tttcctacac
     1261 ttccttgaaa ttcccaatca tctggaaaac tttgtatgcg tgcattttca cgaatagtga
     1321 acggacgtac ttccagtggg tgacagcgct ctgtctgctt ttgagttgga gaggtgagga
     1381 cagtcaaaga gggttcatct aaactcaatc ttcttaaaat acccgtacga ccaccaccca
     1441 tataccaagt tgatttcata tattcttttg caatgctctc tggaatgtct ttccagtatc
     1501 ctcctggagg tactagttca aatatttttc tcttctgttc tgaatattgc attccttgac
     1561 ttggtggaca atcaagtaaa atatccctta atactggctt ataatcatga ggttttggat
     1621 attcaaaaga cgtcatattt actaaatcat tacggattcc aaccataatc atccgttctc
     1681 tcttctgagc aacaccataa tcccatgcat tcagaacttt tgattgcact gtatagcctt
     1741 cttcctcaaa gacattcaaa atagtcttgt aggtcttacc cttatcgtgg ctggtcaaac
     1801 cttttacgtt ttcaaaaaga aacattttag gttgcaactt ttccaagaaa acagcatagt
     1861 gataaaacaa agtaccacga gtatcttcca agcctaaccg tttccctgca taagaaaaag
     1921 actgacaagg agctcctcca ctaagtaagt ctaattctcc ttttttaatt aaaaaatatt
     1981 cttccaaatc aagttttgaa atattagcaa catcatcatg aatcacattc caatttggtc
     2041 gattatattt caacgtttca gaagcagcgc tatcaaattc gactaatcca attgtatcaa
     2101 aacccgcttt ttcaatacct agtgccagac ctccagcacc tgcaaataat tcaattgact
     2161 tcaaatataa ctccttaatt gatttttaat gcgcattcta taacaataat tactagttat
     2221 tacagaaaat aagtgcttgc tttctattat accactattt cactcatagt ataatgaaat
     2281 tcaatgataa agataacctt gaattgaaat ctttaaatac caaaaataaa accccaccaa
     2341 gaataaaact tagtggggtt gtggtgggga actaaaattt ttcccaccat tttatcacca
     2401 tgaaatcgag tttaaaccgt gatttactag gcttccacaa tcaaattgcg gtactctttt
     2461 atttaagagt aactgaagca cctgcagctt caagtttttc tttgatttcg ttagcttctg
     2521 cttctgaagc accttctttg ataactgatg gtgcgttatc aacgatagct ttagcttctt
     2581 taagaccttc gcctgtgatt tcacgaacaa ctttgataac gccaactttt ttgtctccag
     2641 cagctgttaa ttcaacgtca aatgagtctt tagcagcagc agcttcacca ccagcagcgg
     2701 cagcagctac aggagcagca gcagttacac caaattcttc ttcgatagct tttacaagat
     2761 cgttaagttc aagaattgta gcttctttaa tttcagcaat aatattttca atgtttaatg
     2821 ccattgttat ttcctccaaa aatatgtttt aatataaaat tatgtagcta ggctacttga
     2881 agagtgctta tgccacttct tcgtcttttt ctgcaactgc tttgacagca tatgcaatgt
     2941 tgcgtactgg cgcttgaagt actgaaagga gcattgaaag cataccttct ttgtttggta
     3001 atttagcaag agcgttaatt tcttcaactg aagtgaattt accgtctact gaaccacctt
     3061 tgatttcaag agcttctgca tctttagcga aatcgctaat aactttagct ggtgcaataa
     3121 catcttcgtt agaaaacgca actgctgatg gaccaacaaa aagttcttta aggtcttcaa
     3181 gacctgcttt ttcagctgca cgagttaaaa ttgagttttt gataacttta aactcaacat
     3241 cgctttcacg aagtgaacga cgtaagttag tatcttgctc aacagtaaga ccacgtgaat
     3301 caacaacaac gatacttgct gctgctttca ttttctcagc aataagttca acttgttcag
     3361 ctttttttgc aataattgct tcactcatta gttttacctc cgtttttatt ttttgggata
     3421 ggcacaaaaa aatcgcacct aaacctagac acgaaagtac aatacgttat tttataatga
     3481 cgttttgtgc ctcggtagga atattatgag cactgctccc ctactgtctt aggtcagttt
     3541 catcagaaac tttaaatagt ataacttata attctagcat tgtcaataat ttttattatt
     3601 tttctgaaat aattttatag taccaaaaaa tactaaaata gttatcttta tatcaaaaaa
     3661 ataatatttt agtatttatt tattgacctt tactgactaa aggtgtatta tagtaccaga
     3721 aagataatta aatatttaaa ttagacctct ctatatgtat taaagtatag gaatcaataa
     3781 ttaactttca aaaattaaaa tcaattgagg tatttaatat atggcaaata ataattttta
     3841 taatcgtgac ccttttggaa atatggatga tatttttaat tctctaatgg gaaatatggg
     3901 gggctataac agtgagaata agcgatactt aatcaatgga cgggaagtta ctcctgaaga
     3961 attcagtcaa tatcgtcaaa ctggaaaact ccccggtcaa gagctaaata accaaaacac
     4021 tcctacaaac caagtctctg cagatagtgt tttgactaaa ttaggaacta atttaaccga
     4081 tcaagcacgt cagcatttgc tagatccagt tattggacga aacaaagaaa ttcaggaaac
     4141 tgcagaaatt ctagctcgtc gtactaaaaa taatcctgtt ctggttggtg atgcaggtgt
     4201 tggtaaaaca gctgttattg aaggtttggc acaagcaatt ataaatggtg atgtccctgc
     4261 tgctatcaaa aataaagaaa tcatttcgat agatatttct agccttgaag ctggcaccca
     4321 atataggggt agttttgaag aaaatattca aaatatcata aaagaagtta aggaaacagg
     4381 aaatattatt ctcttctttg atgaaataca ccaaatatta ggagccggtt caactggtgg
     4441 tgatagtggc tctaaggggc tcgcagatat acttaagcct gcactttcac gtggtgagtt
     4501 aacggttata ggtgcaacta ctcaagatga atatcgtaac accattctca agaatgctgc
     4561 cttagcacgt cgctttaacg aagttaaagt caatgcacct tctgcacaag atactttcaa
     4621 tatccttatg gggataagaa acctctatga gcaacatcat aatgttgttt tacctgactc
     4681 tgttttgaaa gcagctgtag atttatctat tcaatacatc ccacaaagaa gtctccctga
     4741 taaagcaatt gaccttattg atatgactgc tgcacaccta gctgcgcaac atcccgtaac
     4801 tgaccttaaa tccttagaaa aagaaatcgc tgagcaaaga gacaagcaag aaaaagctgt
     4861 caatactgaa gattttgaag aagctcttaa ggtcaagaca cgcattgaag agttacaaaa
     4921 ccagattgat aatcataccg aaggacaaaa agtcactgca actatcaacg atatcgctat
     4981 gtcaattgag cgtctaactg gagttcctgt atcaaatatg ggtgctagtg atattgaacg
     5041 cttaaaagaa cttggcaatc gtctaaaagg taaagtcatt ggtcaaaatg atgctgttga
     5101 ggcagttgcg agagctattc gccgaaatcg tgctggtttc gatgatggta atcgtccgat
     5161 tggtagcttt ctatttgttg gaccaactgg agttggtaaa accgagctag caaagcaatt
     5221 agcttttgat atgttcggat caaaggatgc tatcgttaga cttgatatgt ctgaatataa
     5281 tgatcgtaca gcagtttcta agctaattgg agcgactgct ggttacgttg gttatgacga
     5341 caatagtaat acactaacag aacgtatccg tcgaaatcct tattctattg ttcttcttga
     5401 tgaaatcgaa aaagctgacc cgcaagttat cacgcttctc cttcaagttc tagatgatgg
     5461 ccgtttaact gatggtcaag gaaacactat taactttaaa aatactgtta tcattgcaac
     5521 ctcaaatgca ggttttggaa acgaagcatt tacaggtgac agcgataaag acttgaaaat
     5581 tatggaacga atttctccat atttccgtcc agaatttcta aatcgtttca atggtgttat
     5641 tgaattctct cacctaagca aagatgactt aagcgaaatt gtagatttga tgcttgatga
     5701 agttaaccaa acaattggca aaaaaggaat tgaccttgtg gtagatgaaa atgttaaatc
     5761 acacttaatt gaactgggtt atgacgaagc aatgggagta cgtccattgc gccgtgtcat
     5821 cgagcaagaa attcgagatc gcatcacaga ctactatctc gatcatacag acgttaaaca
     5881 cctaaaagct aatttgcaag atggccaaat cgtcatttct gaaagataaa aacaaaaccc
     5941 gtttggaaga atccaagcgg gttttctagt ggattgaaaa aggaaatcaa acttacccta
     6001 accttcttca agatacttta cttattttaa atgtgcagag aggtcagcaa tatcagctgg
     6061 tctagttcgg caacaacctc cgacaacttg ggcgccaagt ttttgccagt cacttgtatt
     6121 ttcaagtagg gtgtgtgaat gatcagggct agattgccaa gattggcttg ccccatcata
     6181 aacctctcca gagtttggat aggtcacaag aggcttgtta gtctgctctg ctattgcttg
     6241 caagaaatca gcaactaacg atggacttga acagttaatg cccagcgcca cgacctgtgg
     6301 gctaacatca atagctttcg ccaagtctgc aacagcacta ccatctgaga tggtcttacc
     6361 atcttgagag gtgaatgaca tataagcctc aacctgaggg aaatcctcgg caagtagctc
     6421 aataagagcc tcagcctcct gagcgtttgg aatggtttca agtgccagaa tgtcaacacc
     6481 ctcatctaaa agaagctcaa tacgatggcg atggaagttt ttaagacctt gcttatcaat
     6541 atcgtagagt ccagtgtact cagagccatc cgctaaaaag gcagcgtagg gaccaacatc
     6601 cccagaaatc aaaggataga ttctctctga tttttcttct tttgtcaaac ttttccaaac
     6661 ttgctcacgc gcagcttttg ccaactgaac agtcaagcga atcaaatctt ctgcctgact
     6721 ttcagagaca ccaacttgag ctagtccttg taaagtcgcc tgataagttg atgtcgtcac
     6781 aatatcagcg ccagcacgaa tataatcttc atgaatggtc tgaatagctg ctggatcctc
     6841 aatcagatat ttggctgacc aaagtttccc agaaacatca caacctctac tctcaagctc
     6901 ggtccccaag gcaccatgta ggatgagagt ttttttactc tctaaaagtt ctttaaatct
     6961 tcccataaga tcaccatttc ttgtaacgta gtttgtgata aatatagcag aaaataataa
     7021 atggaacacc gaagtaaagc ccagcacgtt gtgagctatc ccaagcaatc cctacaatcg
     7081 aaatcaagag caaaatgatt gtgatataag ggagaactgg tgtaaaaggt gttttgtaag
     7141 acaaatcttc tagttgattc tctttcaaga attccttgcg gaaattgatt tgtgcgactg
     7201 gaatggctag ccatacgaca acaacagcaa agcccgcgat tgaaaccaag gctagataaa
     7261 ctgtgtctgc agcgtaaata cttgaaaaga gcgacagcac tgctcctgcc attgacaaga
     7321 gaagagcacg cattgggaca ccgtgtttat tgattttcac aacagatttt gacaacatac
     7381 cttcattggc aagggaccag agcatacggc ttgatgcgta gagacctgag ttaccagcag
     7441 acaggatggc tgtcaagata acgaagttca tgatatccgc cgtaaaaggg attcccatct
     7501 tgtcaaagac atcaacgaat ggtgctgtgg atacgcctgc ctctttcatt ggcaatagcg
     7561 aagctaggac aacaattgtc agtacaaaga aaacaaccaa gcgaccgatt gtcgttttaa
     7621 tagcccttgg tacagcttct ttgggattat ccgtttcacc agccgcaata ccaattaact
     7681 cagtaccaga gaaggcatag ttaacagcca acatgactga gacaactgca acgataccat
     7741 ttggaaaggc accattggca gtcagatgag tgaagagaat agccttgtgc tgaccttcaa
     7801 aggaaactag accaaacata gcacctaagc ccaagataat aaagataatg atagcaataa
     7861 ccttaatact tgagaagaaa gactctgctt ctgcaaaaaa gcgtacgcta agagcattta
     7921 aaccaaaaat cacaagggca aaaaaggaag caaatgccca agccggcaca tttgggaacc
     7981 agcgctgcat cagcatggca gcacctaaaa attcagtccc caaggcgacc gtccaacaaa
     8041 tccaatatag ccaagcaaca gtaaaacctg ttccaggact gataaactta gtggcataag
     8101 tgtggaatga ccccgtcacc ggcatggcaa ccgccaattc cccaagtgat agcatgacca
     8161 aataaaccac aacggcacca atcaaataag acagcacagc tccaagcgga ccagcctgtg
     8221 caatggtata acctgaactc aagaaaagcc cagtcccgat aacaccacct agcgataaca
     8281 taaagagatg acgactggtc atcttacgct ggaatttacc ttcatcttca taacgatgat
     8341 tttccataac aacctccaag aaactaatac ttttatccta ccacaatcta gtcgtattgg
     8401 ggaataggta ttagctatca tatatgataa agaaaaacta ttacaaaaaa tcagcgaatc
     8461 ataaatagcg gtaaccttat ttttttatcc ttacaaaatt aacgacctta tgaaaacggt
     8521 ccatttgcca aggactaata atataattgg tctccttatc cttcaatagc tgtacaggtg
     8581 caataatgct agttagtaca cagacaatca acatgataaa accacctagc caatctcccc
     8641 aagtgagcat tccttctgca ctagcttgat agatgtatgc gcaagctatt ggtactagga
     8701 gaaatactgt cgttgctaga ccaggattat accaagtttt aagtttaata ttcatctgaa
     8761 aaccatgacc caagagctgc gttagactaa agaacatgac tcctaatcca agccaataag
     8821 cttgaggaaa agcaatacta gcaatgtaaa gcaaccaagc aatagtatta accaacataa
     8881 tagactgagt attgcctgga aaacaatcca tcagctcttc ttcatcataa acaacatagt
     8941 taatgatagg tgatgcccca ccgggaaact gatattcttc aaattgatgc gctagaagtg
     9001 ccataaagta aaaatacaat agatagtgaa gcattgacca gtcaggtcta aaaacaagca
     9061 atattactgc taaaataata aataaaattg ctccaaaacg ataccagtaa tttctaaaat
     9121 atgacatgat aatacctcct atttaaactg tgtttcacga aaatgtgtta aaaatggttt
     9181 tgagcgtgaa agatcaacat cactaaatgc gtaatttgaa tgtcgaccta aacgcttata
     9241 gattggggaa cgaaaggcta taaagtagtt aagaacaatc catatcagcc taaaaaccaa
     9301 tcaagtccag aatacaactt ccaatcatga gctaaataat agacagaaac agggacaaga
     9361 cctactaagg tcgtaagaag tcctggatta taccacttct tcaaggaaag attaaaaaag
     9421 aaaagatgca tggcaagttc tgcaaatgca aaaatcatga ctgcaagtgt taaaaatggt
     9481 atattgggac aaaaaatcgg caaaagataa acaatcactg caaaccactg attcccgaaa
     9541 aaggctgata gatggtttaa gtcccactta ctagtatcag tttcaaccga tttaagttca
     9601 actagcatag tgataagcgg aaaacctcca gggaaaccaa attcttcgaa aaagtgtaat
     9661 tgaataaaaa gtcccgaaat aagtagtgac tgcactcgta aatctcaatt tccaaaaaat
     9721 aatgctaagg caaaaagaga agcaagatag ggacaaaaga ggtaccattt tgttatcaat
     9781 gctttcataa gatgacctcc aagatttgtg acatttgata ccatatagta taatcaagtg
     9841 aacctataat atcaatacct ttggaggttt tatgcacaaa tgactgaaag tgtgacagtt
     9901 aataaaaaga gaataaaaca aatcactcaa acacaagagg atatcttaac agcttttggc
     9961 caacttttgg aagagtatcc ctatgagaaa attcagatta gccaaattgc caagaaatct
    10021 ggctatgcta gaaggacctt ttaccgacac tttgacagtc gagatgacct acttaccctt
    10081 ttcatcgaaa gactaacatt aaatctcttt aagcaacttg gtcaattgga gcaacctact
    10141 tttagccaag ttttccaaaa tttttttagt tattggtctg attataaatc ccttcttctc
    10201 attcttagaa agaatgacct actccctcaa tttcaacaat cctggttccg acatattgat
    10261 ttgatagagt taggtcgtgg tgatttatct tccaatacct atgctcagcg atttgcgatt
    10321 ggtggtattt tcagtgttct aattgagtgg attcatcagg actgccaaac aagtatcgaa
    10381 gaattaactc aagtttcctt cgatattatc aatcatttaa aaaattaaaa agagctttgc
    10441 agtagtctca acagctactg caaagctctt tttgtcataa actctcaaat tataactcag
    10501 ataaaatagt atcccatgat tcatcaagac ctgtgcggtc aacagatgaa aagacgatga
    10561 aatggtcctt tttatcaaag tttaatttct tcttaataga cgactcatgt ttattccact
    10621 tgccacgagg tattttatct gcctttgtcg ctacaataat tactggaatc tcatagtact
    10681 taagaaattc atacatttga atatcgtccg cacttggatc atgacgaaaa tcaactaaac
    10741 taacaacaac acgaaggtta tcacgtgtta caaggtactc ttcaatcatc ttgccccact
    10801 tagcacgctc agttttagac accttagcgt agccatatcc agggacatcg acaaaacgca
    10861 gtttgtcatc aatgttataa aagttgagca gttgtgtctt acctggctta ctcgacgtac
    10921 gcgctaggtt tttacgacca agaagcgtgt tgataaagct tgatttacca acatttgaac
    10981 gaccagcgag agcaacttct gggagatcat cctgaggata atgtgacttg ttagctgcac
    11041 tcaataaaat agacgcatta tgagtattta aaaattcttc tgccatctct atctccctaa
    11101 gccgtctcta aaacaggctt gtcagtaccc tcaacagcag cctttgtgat acgaactttt
    11161 gttacatctt cttggcttgg aatttcaaac atgatatcaa gcatcgtttc ttcaataata
    11221 gaacgtaaac cacgtgcacc agtcttgcgc tcgatagcct tatcagcgat agccaataga
    11281 gcatcctggt caaattccaa ttctacacca tcataagata ataaggtttg gtattgttta
    11341 accaaagcat tgcgtggttc tgtcagaata cgaaccagat cttctgcagt aagaagttct
    11401 aacgctgcaa ctactggtaa acggccaata aactctggaa tcagtccaaa cttttgaata
    11461 tcctcagaaa ttatctcttg catataagaa gcgttgtcat caattttacg gcttgtctgt
    11521 ccaaaaccaa taactttttc gcctaaacgt tgcttcacaa ggtcttcaat accatcaaaa
    11581 gcaccaccga caataaaaag gatgttcttg gtattaattt gaatcatttc ttggttagga
    11641 tgtttacgcc caccctgtgg gggaacactt gctaccgtac cctcaataat tttaagaagg
    11701 gcttgctgta caccttcacc agacacatca cgtgtgatag aaacattttc gcctttctta
    11761 gcaattttat ctatttcatc aacgtagata ataccacgct cagcacgttc gacattataa
    11821 tcagcagctt gaatcaattt aagaagaata ttctcaacat cttctccaac gtatcctgct
    11881 tcggtcaatg aagtcgcatc tgcaatagca aacggtacat taaggctttt agccagtgtt
    11941 tgtgctaaga aggtttttcc tgagccagtt ggaccaatca tcaaaatgtt ggatttttgc
    12001 aaatctacat catcgtcact actctcggta taactaacac gcttgtaatg attgtagaca
    12061 gcaactgcta aagcacgttt agcacgatct tgccctacaa catattgatt taatatttct
    12121 aatagttcct taggttttgg tacttctgct aaatcagcca gtacttcctc agctaattct
    12181 tccttaataa tttcttgtga taaggccaca cattcattac aaatgaaaac accattacct
    12241 gcaataattt tttttacttc atcttggctt ttgccacaaa atgaacaata gacattcata
    12301 tcgttattac gattcccagc cattttttcc tcaacttttc tattcattta atttaaaata
    12361 atgttccata aaaggcatgg attgtgttga caaggttagt gaaagcattc atcaaaaaaa
    12421 ctgttcctaa tccatatctt tttttcttta tagcaggaat tgcacataaa acacaaatca
    12481 cagctaaaaa tgcggtaaag accccaaaaa ggctacgttg cattgaatag tattccttcc
    12541 ttatttttca aaatatgtca cagtaaaagc atgagcattc tgctcatcct tttcaaaata
    12601 atctctagag ataactttaa actcagatag attaacatca ggaaaatagg tatcaccttt
    12661 gaattttcca tgaactttag tttttatgat tgcttcacaa taaggcagaa atgctttata
    12721 aatacttgca ccacctacaa taaataaggt cttattatgt tcctgaaacc attttataac
    12781 ttgttcaaca ctatttagga ctgtcactcc atctgcttgg aattgttcat cttttgttaa
    12841 gatgattgtc tctctaccag gtaaaacacg acggttcatt ccatcaaagg tctttcgtcc
    12901 cataagcaaa gcctgcccca tggtcgtttc tttgaagtga tgtaactctt taggaagcct
    12961 ccatggtaaa ccgccattaa ctccaatcaa atggtcttca tcttcagccc aaatggcaat
    13021 tatttgcttt gtcatgactt ttttcctcac taacttatct ctagatattc taacaaattt
    13081 tacctcaaat tgcttgttaa aaaacatgag atagttagat tgctaaatca aaacgtaatt
    13141 gaggtttaat tgggtcataa tcaactaacg caaaatcatc tggtttaata tcaaaaaagt
    13201 ctgttccatc tggaacattc aatactagtt tggggttaca ctcagacggt tggcgtttta
    13261 agagttcctg agcttgttca aactggttat cataaatatg aagattattg ataaaataga
    13321 aaaacttccc aactctccaa ccaaaatgct ttgcaatcat catctgaagt gctacatact
    13381 gcatagcatt aatatgatgt gcaactaaca tgtcattaga acgttgtgtt aatgtggcat
    13441 ccaaataaag ctcaccatta acgcgtctca catcaaacat cgtttgaaaa gcacaaggta
    13501 gtaggccttc tgtttcttca aatgcttcat agtcccataa tgaaataaca ttacgacgat
    13561 tccaagggtt atcttcaagc tgttttaaga gttttgatat aatattatgt tttttaacaa
    13621 ctgcaccgta gcgctgacct atcgtgccag tatggccaac ttcccaatca ttccagtatg
    13681 tcacaccata tttatcattc aaaactgcta aatcatttgt ctgatcttga taaatccaga
    13741 aaatttcttt aatagcagat tttattggaa ttggacgtag agttgtaata ggaaattcac
    13801 cctttgataa atcatactct gcaaaggcac ctgtaatata cttagaatta gccatttccc
    13861 catttttata tctaggtctg gcttgctcgc taaatacacc ttcagacata attttcgtga
    13921 tattgtcttt aaataataaa tcggcttttg tcatgagata cctccaaagt cttattttat
    13981 cagattttaa aaagatattc tagtgagaaa caagactttg aaggttaaag ctttttttca
    14041 tatttttagc ttctttatgg taaaatggaa tttagcaaaa atgactttta ttcagaaagg
    14101 tttaccaatg aaaataggaa ttgataagat tggttttgca accagtcaat atgtccttga
    14161 aatgactgat ttggctattg ctcgccaagt tgaccctgaa aaattcagca agggattatt
    14221 gttagattca cttagtatta cgccagttac agaagatatc gttacacttg cagcttctgc
    14281 agctaatgat attttatctg atgaggacaa agaaactatt gatatggtga ttgtcgctac
    14341 agaatcaagt atcgatcaga gtaaggcggc ttctgtctat gtacatcaac ttttagagat
    14401 tcaacctttc gcaaggtcat tcgagatgaa agaagcatgc tatagcgcaa ctgctgctct
    14461 tgattacgct aaattacatg tagaaaaaca tccagattct aaagtattag tgatagcttc
    14521 agatattgct aaatatggca ttaaatctac tggagaatcc actcaaggtg caggcagtat
    14581 cgctatgctt attagtcaaa atccatccat tctagagcta aaagaggacc atctagcgca
    14641 aacccgtgat attatggact tttggcgacc aaattattct gatgttcctt atgttaatgg
    14701 catgttttca acaaaacaat atctagatat gctaaaaact acttggaaag tatatcaaaa
    14761 acgttttaat actagtctaa gtgactatgc tgctttttgt ttccatattc cttttccaaa
    14821 attagcttta aagggcttta ataaaattct agataataac ttagatgaac aaaagaaagc
    14881 tgaactacaa gaaaattttg agcactctat tacctatagt aagaaaattg gaaattgcta
    14941 tactggctca ttgtatctag gacttctatc gttactagaa aatagtcaaa atttaaaagc
    15001 aggtgatcaa attgccttct tttcatacgg tagcggagct gttgctgaaa tttttaccgg
    15061 tcagttagta gacggctacc aaaataaact tcagagcgat cgtatggatc aactcaataa
    15121 acgtcaaaaa attacagtaa ctgagtatga gaaactcttc tttgaaaaaa caatcctaga
    15181 cgaaaatgga aatgctaact tcaacaccta ccggactggc actttttctc tagactctat
    15241 ttgcgaacat caacgtatct acaagaaaat taataattaa tatgacaaaa atttcctgga
    15301 caggattttc aaaaaaatcc cctgaagaac gtattcatta ccttgaagaa caagattttt
    15361 tagctgattc ttccctagaa atagtgacga atcaggactt actctcttta tcactagcca
    15421 atcaaatggc tgaaaatgtc atcggtcgaa tagccttacc attttcactt gtcccagatg
    15481 ttttggttaa cggtaaagtc tatcaagtcc catatgtcac tgaagaaccg tcagttgttg
    15541 ctgctgcaag ttttgctgct aaaattataa aacgatctgg tggttttctg acaaccgtcc
    15601 ataatcgtaa aatgattgga caagtagccc tttatgatgt acaagatagt caacacacta
    15661 aagaatctat attaaatcaa aaacagcaat tattagaaat agccaacgct gctcatccat
    15721 caatagtcaa gcgtggaggc ggtgcttgcg acctaactat tgagattaaa gaagactttt
    15781 tgattgttta ccttatggtt gatacaaaag aagccatggg tgctaatatg gttaacacca
    15841 tgatggaagc attatcttcg ccattagaag acatttcaaa aggcaaaagc ttaatgtcaa
    15901 ttttatctaa ctatgctacc gaatctttag tgactgctac atgccgagtt gatttaagat
    15961 ttctaagtcg ccaaaaagag gaggccatca agttagctca aaaaatgacg atggcaagtc
    16021 aattagctca agtagaccct tatcgtgcta gcacacataa taaaggtatt tttaatggca
    16081 ttgatgctat cgtcttagct actggaaatg attggcgagc tattgaagct ggagcgcata
    16141 cctatgctgt caaagatggg caatatcgag gattatctag atggtcttat aaggttgatg
    16201 ataattgcct agaaggtact ctgacgctac cgatgcctgt tgcaacaaaa ggtggatcca
    16261 ttggtattaa ccctagtgtc cacctagctc acgatctact aggtcgtcct aatgctaaag
    16321 agttggcaag tattattctt tcgattggac tagctcaaaa ttttgcggct cttaaggcat
    16381 tggttagcac aggaattcaa gctggtcaca tgaaacttca agcaaagtcg ttggcactac
    16441 ttgctggagc taaagaagaa caaatttcag aagtcgttaa acagcttcta gactctaaac
    16501 acatgaatct agagacagca caaaaaattg tcaataaact tactaaaagt aaaaaaagaa
    16561 tttgagaaaa atcatctcaa attctttttt tacttgactt gaatttccat aagtgtataa
    16621 tctagttata aagactagat tatatggagt tttaaaatga ataaaaatca atacccaaaa
    16681 tgggaagaat tacctgaact agatttgtat cttgaccaag tacttctcta tgtgaatcaa
    16741 ttaattaatc ctaaaacaat aactaacgat aaactactca ccgcttcgat gatcaacaat
    16801 tatgtcaaac ataattatat atccaaacct ataaaaaaaa aatacaatcg ccgtcaagta
    16861 gcacgattga ttgttattac agcttttaaa caaatttttg ctattcaaga aatcagtcaa
    16921 acattagaac ttctgacagc tgacaatcat tctgaagaag catataacgg atttgctgct
    16981 tgtatgaaca aagaagaggt tcacgattta ccaccagttg tcattagtgc ctgtcagacg
    17041 ttgaaccttt atcaagaaac acaaaaatta gttttagaac taggaggaaa aaataaaaat
    17101 gcttaaagaa atgtctatca aattaagtcc tcaactttcc tttggcgaag agcttgcaaa
    17161 tagtatcact cacgctgtag gagctttgct tatgctaatc ctattaccaa tcaccgctgt
    17221 ctatagtcac aaccacttcg gattacaagc agcacttggt acctcaatat tcgtcacttc
    17281 tttatttttg atgtttttat catcaagtat ttaccactcc atgacttata acagtcttca
    17341 aaaatatgtt ctacgcatga ttgatcactc tatgatttat attgctattg caggtagtta
    17401 tactcctgtc gcattgtcac ttattggcgg ctggttagga tatctcatca tttttttaca
    17461 atgggggatt acattatttg gtatccttta taaaatcttc gctcccaaaa tcaatgataa
    17521 atttagcctt gtactttacc tcattatggg atggctggtt atctttattt tccctgctat
    17581 tatcacaaaa actggaccag ctttttgggg attactcttg gctggtggta tttgctatac
    17641 tatcggtgca ctcttttatg ctcgcaaacg tccttatgat cacatgattt ggcacctttt
    17701 tattctatta gcttctattt tacaatacat tggaattgtc tacttcatgc tataaaattt
    17761 aactacattt tattgattat ttttttgcta aaccatgata taataataat catctaaatc
    17821 ttagagagaa ggcgtggaca tatgaaaagt gcttatatct tttttaatcc aaaatctggt
    17881 aaagatgaac aagcattggc aaaagaagta aaatcttatt taatagaaca tgattttcaa
    17941 gacgattatg ttcgtattat tacaccttca tctgttgagg aagctgtcgc cttagcaaaa
    18001 aaagcatctg aagaccatat cgacttggtt attcctttag ggggagatgg cactattaat
    18061 aaaatctgtg gtggtgtcta cgctggtggt gcttatccta ctatcggact agtacctgcc
    18121 gggaccgtca ataacttttc aaaagctttg aacatcccgc aagagaggaa tctcgctctt
    18181 gaaaaccttc taaacggtca tgtaaagtcc gtagatatct gtaaagttaa tgacgattac
    18241 atgattagca gtttaacact tggtctcctt gctgatattg ctgctaatgt cacttctgaa
    18301 atgaagcgta aattaggccc ttttgctttc ttaggtgatg cctaccgtat attaaagcgt
    18361 aatcgatcat attctatcac tcttgcttac gataataatg tacggtcttt acgcacgcgg
    18421 ttactcctta ttaccatgac caattcgatt gccggtatgc cagccttctc tccagaagca
    18481 acaattgatg atggactttt tagagtctac actatggaac atattcactt cttcaaatta
    18541 ttgttgcatc taagacaatt tcgtaaggga gattttagtc aagcaaagga gataaaacat
    18601 tttcatacca ataatctgac tatttcaact tttaaacgta aaaaatcagc tatccctaaa
    18661 gttcgtattg atggcgatcc aggtgaccaa ttacctgtaa aagttgaagt catccctaaa
    18721 gccttaaagt ttatcattcc taactcatta ccataatata aaaggaccta gacataagta
    18781 ttagagtaaa tactatctgt ctaggttctt ttttgttata cttaaagcaa taaatatcat
    18841 tgaaaggaag tatacgatga caacatttac tgctaaattt attgaccaag agtgggaagt
    18901 cccagtagaa tcaggtcgct accatatgat tgttggggaa ttttgtcctt atgcacaacg
    18961 tccgcagatt gcgcgccaac tgcttggttt ggataaacat atttctatca gttttgtgga
    19021 tgatgttcca agtgatatag gacttatttt tagccaacca gaacaggtca ctggtgctaa
    19081 gtcgcttcgt gatatttatc atttgactga cccgacctac caaggtcctt atactatccc
    19141 aattttaatt gacaagaccg ataatcgtat tgtctgtaag gaatctgcag atttgcttcg
    19201 actttttact acagatttct ctgatttgca tcaagaggat gctcctgtcc ttttcagcca
    19261 agaaactgct tcacttattg ataatgacat taaagacatt aataagaact tccaaagctt
    19321 aatgtataag ttagcttttc ttgataaaca agcagattat gatacttata gtaaggaatt
    19381 cttcactttt ctagatcaaa aagaacactt actaggacaa cgtcctttct tgctcggaga
    19441 taatctaagt gaggttgata ttcatttctt tacaccatta gtccgatggg atattgctgg
    19501 gcgtgatctt ctactactta atcagaaagc gctagaagac tacccaaata ttttttcttg
    19561 ggctaaaact ttatacaatg actttaattt aaaaacatta accaatccac aatccataaa
    19621 aaataactat tacttaggca aatttggaag agctgttcgt catcatacca tcgtcccaac
    19681 aggtccaaat atggcaaaat gggaaaaata aagctaaaaa gctaaggcaa ttcacagcct
    19741 tagcttttta gctatactag tccaaagtgg accaatcaaa ttcaataaaa gtcttgaaga
    19801 gatctccata agtaccactg taagcaatat ctttatcttt ataagtcatt ttcgtaatag
    19861 gataattgtc aggaacagta acacatccca ttaaaactcg aataaaatca tttggatttt
    19921 caaaaacttg gcatcgttca gtgtcgtaag catctttata atacagttta atcatcttat
    19981 tacctctgac gttttacaag gaaactttga caggaagcta tatctacttc acaaaaataa
    20041 tatcatttag cattggcttc cttcaattga ccatacaata tataattgac ttgtcgtaaa
    20101 tcagttattt ttttacaatt cagtgcacac attatcattc ttaaatcttc tttccaagaa
    20161 ttgagaattg caataacatc atctacagga taacgctcaa ctaattctaa aactgttcta
    20221 gaaagtccaa cagctttagc acccaacact aaacatttta ccatatctaa aggatgacga
    20281 atgccaccag atgctaggat atccattttg tccatcatag attgagcgtt tattaaggat
    20341 tgagctgttg tttgccccca ggtattgaga tacgaacggt cgcgtccacg ttgattttcg
    20401 atgtaagcaa aactagtccc cccacgcccc gaaatatcta cagttgtgat acctatatcg
    20461 taagcatcct ttattgattg taaatccatc ccaaaaccaa cctcttttaa aataagtggg
    20521 cattggatat tgtcgacgta ttcttttaag tgagaacgcc acatgtgaaa ttcacgctct
    20581 ccttcaggca tcaaaagttc ttgcatcaca ttaacatgaa cttgtaaaaa aatggggttc
    20641 atagccttaa cggttgactc tgctgctggt acaggcttgt ctaaccctat atttgtcgcc
    20701 agttttaaat caggatacaa gtctgttgtt gggtaggaat catcttcatc gtttttgaga
    20761 gctgccgaat atgaacctgt caccataaca atccctgtag cttgagcaac ctgtgccaat
    20821 ttatgattga ctgctttccc tttttcacta ccgcctgtca tagcattgat ataaaaagga
    20881 aattcaaagc tttgaccagc aaaatgtgtt gataaatcga tatcgttgac attatatttt
    20941 ggtaaggatg aatgtattaa ctcaatatcg tcaaaactat tataatgcga ctgatattta
    21001 agagcatatt ttatgtgatc atcttttcga tttgtcatga cagtgtctcc ttactaagta
    21061 gttcaatacc agcactctcc caacgcttga ttagagtttg attatcattt ttattaaagg
    21121 atatggctat accacaatcg cctcctccag atcctgaaga ttttgtgact atatctaggc
    21181 ctttgcttgc ctctttaagc ttttgaagtt tatcggtgta aatagatgga tgaagattaa
    21241 ataatagcaa gctaatttct tctaaacatc tcttaatcgc ttctttatct ccttctttaa
    21301 aggctgaaag taactggaca acttgatact ttgtatcatc taaaaaacgc tgcgtaatag
    21361 ttgatttaac tatatttatc atttcttttg aaatggacgg ttgcattgtc catcccacta
    21421 aaaaatccat ctccaataaa gcttgtattt tggtaatacg ataaccccat tcagcttcca
    21481 atacttgttc caaaggtttt gtttcaatca tttttgaaac agccctacga tcaaaagctg
    21541 aataagaaat taaatgctca taagcaatac aagcaatatc tcccattgag ccattatcac
    21601 ctctttttaa gagaacatag gcagataatt taaaaagcaa gtcgcttggc atctctatct
    21661 cataaagcgc agccatagct ttaatagtca aaacgacaac acttccgcta gagcctatac
    21721 caaatttaag tccatagcgc tctaattttc ccgtaatttc gaggtgaatc ggttttatat
    21781 ttttgccaaa attaatcaac cattcttcca taagcaatat cgtttcctga atgagactat
    21841 attgcttatc tggctgtaag cttgccgtat agttaaacat atcagaataa aggctgtagt
    21901 tatctgcaaa agtagctaat gcagtcatat aaataggaac gttctttaaa atagctactt
    21961 gcccaggata cagtatcgcg tattcacctg caatatataa tttgccacct gtttgcacct
    22021 taaccatctg ctaattcctt tgtcgttgaa acaataatac gataatcttt ctccaagata
    22081 gcagctaatg cctcaaggtc ttgacgtaag caaagtactt taacatttgg acccgcatcc
    22141 atcgtataat aacattcatg acctttttcg cgaagtttct tcacaacatc catagcttta
    22201 tatgtctctt ctgtcaaata tgaaaaggct ggagttgctg ttttagttgt tgagtgcatt
    22261 gcaagtgcat tacgctctgt cagttgacct accttttgaa aatcattatt ttttaggtat
    22321 actaacatat cttgataatc ttgttcagat tgccttaccc attcattaaa agtagttgaa
    22381 gtttcggtgc agattttcat accttcacgg cttgaaatcg gctttctttt gtcatttaag
    22441 accagcataa tcatagctaa atctaaatta gtgtgaactt tatagatatc accactatct
    22501 ttatcccaag cagcaacagg tccaaaaaaa gagcgtgaag aggagcctga agcaaacttg
    22561 gcttcttgag ctagctgtga ttggctaagg ttagtaccaa aaaaatcatt gcaggccttt
    22621 actaaggctg ataaaccact tgatgacgat gataagcctg cagctgttgg catattatta
    22681 gttgtttcaa tctttacaaa gccggaccta ttttgtcgaa agcgatccaa gatagctgac
    22741 attttgtcat gttcatgatc attttgaaga acaccagaga tgtaaaattc atctttctta
    22801 gcatccttcc caagggcagt caaacgtgtt tcagtgtaca tgttttcaag tgttagagaa
    22861 atactactgg tagctggaat cattttttca gcatctgctt ttccccaata tttgataata
    22921 gcaatgttag cataagactt aacacttata ctttttccgt ccatgtgttg acagctcctt
    22981 ctctttctaa ttgctgactt aagcgctcag cttctctttt ttcttttaca agtgcgataa
    23041 tacaaccacc taatccgccg ccactcattt tagcgcctag agcaccacaa ctaatagcta
    23101 cctctaccag atgatcagct tctaaacttg agacacccaa ttgttttaaa ctttgatgtg
    23161 ctttagtcat caagctacca agttgtttgg aatcactttt atgaatagca tcctctgatg
    23221 cctctgctaa ataacctaat tcttttaaaa agggtagtac tgcctcgcca gatgatttca
    23281 ccttatccac tgcttcttta gtatgcccat gaattcctgt atcagcaata actaaataag
    23341 cattcaaatg catgggtact gttgaaaaac caatattgcg gataaattta atagtatttt
    23401 cactaagaca agtttttgca tctaatccac tcggatttga atgtgctatc atttctgctc
    23461 gattaacaag gcattctaac aaatctgcct ccaaatcttc atcaaaataa tcaaaaactg
    23521 cacgaatagc tgcaatagca actgcagctg atgatcccat accacgtctt tctgggactt
    23581 gtgactcaat atgatatgca attttagaag aagttttacc taaatagtcc aaggcagcaa
    23641 aaatggctgt cgacaaagga tcagtcatat ctaatgctat taattgagga gcctcttcaa
    23701 taaggcaagt tacttcaata ttttttaaag gaatagcaat ggctggataa ccatatacta
    23761 ctgagtgctc acccatcaag ataatcttgc tatgagcctt tcctatacca aacttttctt
    23821 tcatcttttt ctctttacaa ttctattacc ttactatttt aatataaatc cttaaaaaaa
    23881 gcgactttaa cgtgataata acataaatta aaggaactac aattgcagtc cctttaatct
    23941 taaatgtaaa tgacaaaggt aatggtatca tgtttataat tcacatcgat acttccttta
    24001 aatagatgca ccaaactagt tacgatagaa agaccaatac catatcccga ttttttgtta
    24061 ttatgtgact catcctctcg ataaaatcgt tcaaaaaact tgctatagtc tatgtcttta
    24121 ccattcttat aagtatttga aacttcaagc ttagctcgtc tcaaacgact tgaacgtgat
    24181 aacttaactg tcacagtccc cataggatca caatacttat tagcattatc aaccaataat
    24241 gtaaccaatt caaataatga tttttcttca gctttgacat gaatacctgg ttggatactc
    24301 attataaagt ctttaccatc tttaatgatt ggacctttaa aatcttctgc agcatctttt
    24361 gtaatatgtg aaaagtctaa gtcaaccata cttatatcag gctgttcttc aaagcgggca
    24421 agactgacca tgccattaat tagacccgtt aaacgttgga tttgatcatt tgtagattta
    24481 gtccactctg attcaccact catcatttcc accaattcat tattagcgga gatgatagca
    24541 agtggagttt ttagttcatg tcctgcattg gtaataaaac gcctttgctt ttcatagtta
    24601 gcaacaaatg gtaagataac tctccctgat aaaactgaaa caattaccat gaagacgata
    24661 aaacttacca aggacatcca tattgaaagt tgcaataatc tttgattatc aagatattgg
    24721 ttggttgtat ctaaaaatac cactaaataa tcatttttac caaccttttt tagtagataa
    24781 gaataagtac taaaattata ggtgatatga cctgaatgac tattttgtga tacgatacgc
    24841 ttggcataat tttttatttg cctatcgctt acttcaaaga tatttctaga atcaaaacta
    24901 gtcacgttac cttttgcatc tgttcgcgca ctgaaataac ggaattggaa aatagcgtcc
    24961 gttgataggt catcacctaa ttctttacta aattcaagtt tattcggtat acgtccattg
    25021 ttgtctgcaa ggatagttaa cacacgataa atttcattct tcgtttgata gtgattagca
    25081 gagttgagga caccgactac tgaaaataag acaacaagaa tagctagcgc agctattccg
    25141 ataaaacgaa gtcgtaaatt tctaaacatt agccaccatt tatttcactt aatttatagc
    25201 tactaccttc ttgaccaata atctccaagt tagcaccaat tgcctgcaat ttttgtctca
    25261 aataagaaac atagataaag atgtatccct catctacatc ttcttggtcc ttatctgctc
    25321 cccagacatg ttcgaaaagt tgttgtgtag ataattcttt atcatggttc aacatgaaaa
    25381 atgctaacat tttagcttct ttacccgcta gacgaatagt gttttcacat tgcaactctt
    25441 gttcaccgac tgataaggtt actcttccca atgacaatac attaggtgtg aaatcttcca
    25501 atcttcggga catagagcga agccttgcta ataattcttt tagagagaag ggttttgtta
    25561 aataatcatc tgcaccagca tctagacctg ttactctatc atcaatttct gccatggctg
    25621 ttagcataat aatgtgagat ttatttcctg attgtctaat ttctttgaca gcctcaacac
    25681 ctgttttaac aggcatcatg acatccataa ccatgacatc atatgcattt tgattagcta
    25741 aatcaatagc agtctgacca tcgtaggcga catcaacaac ataaccttga tgactaattg
    25801 ccgtactaag gacacgtgac atctgttctt catcttctgc aattaatact ctcattttat
    25861 tccttctttg ttccactctc tatcaattga cgaatcgttt gcatcgttaa atcaacagat
    25921 gctatttcat cagcacaacg ttttaattct cttacaattc gttcgggatt ttcaatatca
    25981 tgcttgtatt tcatctgatg ttctaaacta gcccaagaat cttgcgcaat tgtacgcaac
    26041 tgaatctcca tgtaatattt accttctgaa ttacccaaac agtccggata gggagtatca
    26101 atttctaaaa tcacatggta agagcgatac ccatttggct taacatgctg gatatagtct
    26161 ttttcaacta caatacgaca atcatcaaat gtcttaatac gttctacaat ttgataaatg
    26221 tcgttaacaa aaccacaaat aatacggact ccaatactat ctctaataac ttttagtgca
    26281 gaatctactg ttacaggtaa ctgtttacga cgacatttct ctatcatact atctgatgtc
    26341 ttaatacgac caatgatatg ttcgtacaat ttatagcctg tttcaacctt agctttatca
    26401 ttttctaatt ggatacgctg actgaaatct tctaaaatta atggtagata tcgaccataa
    26461 tctccataaa tatttgtttc cataagaaac cttcctattc tctcttaagc tccattatat
    26521 catagaaact taaggcaaac taaaaaggaa tgagaatttt ttcttcccat ttccttctaa
    26581 aatgtttatg ttaagctgat tcgaattgag aattatacag atcagcataa aaaccacttt
    26641 gagacatcaa aacatcatga ttgccttgct caatgatatt cccatctttc atgaccaaaa
    26701 tcaagtctgc gtttttaatc gttgataaac gatgtgcgat aacaaatgat gttcttcctt
    26761 ccataagttt gtccatagct ttttgaatta actcttctgt acgtgtatca acagatgaag
    26821 ttgcttcatc taagataagt aagggagaat ttttcaaaag agctcgagca atggttaata
    26881 attgcttttg tccaactgac aatgtcacag cgtcatcaag gacagtatca taccctttag
    26941 gtagtgtcat tataaaatga tgtataccga ctgccttagc agcttcttga acctcatcat
    27001 cactgattgt cgtctggtta aaaatcaaat tttctttaat agttccctca aacaaccatg
    27061 tatcttgaag aaccatcgag aaagcatcat gaacttgact acgtttcatg tcttggatat
    27121 tgatgtcatc tatggaaata ctacctgaat taacatcata aaaacgcatc aataaattaa
    27181 caattgtcgt tttacctgcg ccagttggtc ctacaatagc aactttctga ccaggctgtg
    27241 cctccgaagt aaagtcatga ataatagtct tgttatcact ataaccaaac ttgacatgtt
    27301 caaaggacac atgcccctta aacttaggaa gatttaacac tttgtgttta tcctcttgca
    27361 tatttgtttc atttaaaaat tcaaaaacac gtcccatggc tgcactagca gactgtaatt
    27421 gtgtaattcc ttgagcaatc tgaccaatag gctgtgtaaa aatacgaaca taggtcataa
    27481 aagcaacaat tgtacccata gtaattgttc ctttgatagc taacatggca ccaaccacac
    27541 acaccatcac ataaccaaaa ttaccaataa actgcatcag aggcatcata attccagaga
    27601 aaaattgtga tttccacatg gaactgtgaa gtttatcatt taatagttga aaatctgact
    27661 tagcctgctg tcttgcatta tgactagtaa caatgttgtg tcctgagtaa atttcttcta
    27721 catatccaga tatattagcc aaattcacct gttgcttttt aaataaaggc tgactcttca
    27781 ccataatgat tgaagaaaga gcgaatcctg aaaataccga tataatcgcc gttgttgcta
    27841 aatgccaatc tgttttgaac atcataatta atgaaccaac caataacact gtagatgcaa
    27901 ccatagtaac taaactttgg ttaaaagact gtgtcatcaa atccacatca tttgtaacac
    27961 gagataaagt atcaccttga gtatggctat caaaataact caaaggtatt ttattgattt
    28021 tatcagcaat agcattgcgc ataccttgag caaatttttg aataatagtc gtcacaataa
    28081 aacttgctag ataggatact aatgcaccta aaacatataa tactgccaat tgccctgcaa
    28141 tatgaccgat tttggctaag tcaatattac cagccaagcc ttttaccata agatccgtca
    28201 tctctttaag acggtctggt cctataactg ttgtcaagct tgataaagtt gctaacacaa
    28261 ttgctaacgc aaaaagaagt ttgtaacctt tgaggtacgg agataaatta gagaaaaaag
    28321 acgttttttg tgatttagtt ttcattttcc aattcctcct tagacaattg agaataggct
    28381 atctcttgat atacttcatt atttgataac aattccttat gtgttccttg cccaacaact
    28441 ttaccttgat ctagcactaa aatgagatca gcatccatga tagttgaaat gcgttgagcg
    28501 acaattaatt tcgtcatagc agctgttttt tctttcagca catggcgtaa aatatgatct
    28561 gttttataat ctaacgctga aaatgagtca tcaaaaatga gaatttcagg gtgacgtgct
    28621 aaagcacgtg cgattgccaa gcgttgtttt tgtcctcctg aaaagttact accaccttga
    28681 gcgacttctg tatctaatcc tttttcttta gcttctataa attctttagc ttgcgctaat
    28741 tcgagtgctt gccaaatttg ttcatcacta agttcctccc tgccactctc tccaaaaatc
    28801 atattagaac gtactgtccc actaaagaga acagctttct gtggaatata gccaatttta
    28861 ttattcaaat cattgtgaga gtattccttt atatcaatgc catcaagttt aattgacccc
    28921 tctgtggcgt cataaaaacg tggaactaaa ttgactaaag tggattttcc agaacctgta
    28981 gacccaataa atgccactgt ctgaccagta tctgctttga aagatacatg ttcaataacc
    29041 gcttcagaat ctttactata atgaaatgac acatcttgaa actcaatttg accttttggt
    29101 gcactttgga tatgactatt ttctttaaaa gttactgttg gggctaaagt tagtacctca
    29161 ttaatacgtc tagccgatac taaagcacga ggcatgatga taaaaatagc taccatcatc
    29221 ataaatccca taacaacttg catagcataa gatgaaaaaa caaccatgtt tgaaaaaaga
    29281 tccactcgat cagtgatagc tgatttagca gccaatacag atacacttgt tcctgtaact
    29341 ttaggcacag aaatatcatt aatcaaataa gagccaatcc aataaatagc taaagtcaat
    29401 ccagatgaaa ccactgtcat cacaggattc ataagcgaca tcaatctata aacaaaaagg
    29461 ttcaattttg ttaaatcgtc attttcacgt ttgaacttat ctgtttgata cttttcagca
    29521 ttgtaggcac gaataactct aacaccagag agtgcctctc tagtagttgc attaagcgca
    29581 tctgtaaaac cttggatttg acgttgtctc ggaaaagcaa gtaagagtag aacgctaagt
    29641 aatatcataa cgataataac cgctactcca actgccccag tccattgatc gcttttaccc
    29701 ccaattttag tcaaggccca tattgccata attggtccac gggtaacaac ctgcattccc
    29761 attacaataa ggatctgtag ctgagtcaag tcgttagttg tgcgcgttaa tagagagggg
    29821 atagaaaatc gcttaatttc tgcatcagaa taatccatca catggttaaa aatatctgaa
    29881 cgtaatcgac tagtaaaact agctgctgtc ctagcaccta aaaaacctac cattactgct
    29941 actaaaaaac taccaaatga aaaagcaagc atttttagtc caggatccca aagagcctct
    30001 aactttgtac ctttagtttg caacatacgt gtgatatcag acatatattc tggagtcttt
    30061 aggtccatcc aaaccgctag acacactaat atagtactcg ccacaatcat caaccattct
    30121 ttaccattga gtcgcttgaa aatattaaac atcgcaaact ccttttactt tcatatttat
    30181 cctattatcc taaaatagtt aatcaaaatc ttccatcgcc atatttttag ctaatgttct
    30241 aataaccttt ttggtagtac gtatctcttc tttagtaata cctgctaaca tggtactgtg
    30301 caatttttct aagaaaattt caaattgtgt agccttttgt ttacctagat gagtcaaata
    30361 aaggtatttc acacgcttat ctgtcttaga tggaacaatc gcaataaatc catttttttc
    30421 catccgtttt accaaattag acgctacaga cttagaaatg tgtaaaattt cttcagcatc
    30481 ttttatagac atatccttgt caggatgttt atagagatgc atcactaagt gtccttgtgg
    30541 acccgctaaa tgttcaacat catactcttt tgcataacga tctaaatact tttcaagttg
    30601 attaacaagt attcgtgctt tttgaagagg attctccata ataatactcc tttaatagtt
    30661 atcgtgagaa ctattttaaa acaa
//
LOCUS       DATRUK010000019        28360 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.019, whole genome shotgun sequence.
ACCESSION   DATRUK010000019 DATRUK010000000
VERSION     DATRUK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28360
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.019"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            5..829
                     /locus_tag="VBE34_000822"
     CDS             5..829
                     /locus_tag="VBE34_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEO7263551.1"
                     /translation="MDLITRLKVERDGRVYGGIYDITQKRFAFNSNKIEGSRLTEEQT
                     SFIYETKTIANIGGTGIKIDDLVETTNHFKCFDYIINTVDEQLTEDYVKKLHSILKAG
                     TSSEYNEYVPVGRYKVFENEVGQIATAAVDQVEEEMYSLLLGYNFKKEKDLAYLASFY
                     ARFEQIHPFADGNGRVGRLILYKECLKEGITPFIVDDTRKADYYNALEQFQVYDNHQP
                     LIDYFAIEQKFYANYLKNKGFVGKINDSKDRDVINKKSKSADDSHKRPNKTKGFSL"
     gene            911..2260
                     /locus_tag="VBE34_000823"
     CDS             911..2260
                     /locus_tag="VBE34_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
                     /protein_id="HEO7263552.1"
                     /translation="MTGMRFDEKVFVVELDDKRKYDFLKRVKTYDANIASAMRIAGYK
                     RINQTERTVAFTFGEVTFSRSRWRKGNKTRCPIDEWLGLKKYMRFSPELICHIAKMAT
                     VMSYRQVCHFVKEFYHLEITKDTVLKAVKRAGDLLSEKEKFRFWLEEEPTKKIKPKIL
                     YIEGDGVMVKSSDSARDDKRNVDLTHFLVHTGSKKIAQNRYVLENKHEIIHTNYEKAK
                     EELLDYLYNHYEITDETILITNSDNGKGYTPKVFQEIKKALGIKRHEHFWDAFHLHEK
                     IKIFFKKYPAELKELAFEAINTHKRELMKTVLDTTESLIDGEGDEYYNYFNFRRKILN
                     NFKYTKPAKLRNLSSKGIGVMESQHRKITYRMKHRGMYWSLKGAYTMSRLIILERINL
                     VDDLFFGDWRKEYQYYQNQRFSVGYLKEIKAETIIKTRKIFQRAGKFTSLDRQKFKY"
     gene            2682..2816
                     /gene="rpmH"
                     /locus_tag="VBE34_000824"
     CDS             2682..2816
                     /gene="rpmH"
                     /locus_tag="VBE34_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEO7263553.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            3172..3846
                     /locus_tag="VBE34_000825"
     CDS             3172..3846
                     /locus_tag="VBE34_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7263554.1"
                     /translation="MRLLVVEDEKSIAEAIQALLADKGYSVDLAFDGDDGLEYILTGL
                     YDLVLLDIMLPKRSGLSVLKRVREAGLETPIIFLTAKSQTYDKVNGLDLGADDYITKP
                     FEADELLARIRLRTRQSSLIRANQLRLGNIRLNTDSHELESKESSVKLSNKEFLLMEV
                     FMRNAKQIIPKNQLISKVWRPSDNSEYNQLEVFISFLRKKLRFLKADIEIITTKGFGY
                     SLEERT"
     gene            3846..5033
                     /locus_tag="VBE34_000826"
     CDS             3846..5033
                     /locus_tag="VBE34_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7263555.1"
                     /translation="MFSDLRKKFVFLTMSILIVVVLFLFAVSNRYNQYWDEYDAYRIV
                     KLVAKNDYLGIPGDEPIALVTIDNQKMVKIQSNNTDLTNDVIEKSSLKLLEQGKKSRK
                     WKSFIYSIKEYKDKTYTIAIMDLASYEVPYARRFLILVFTIFGFCLLAAVSLYLSRFI
                     VGPVETEMTREKQFVSDASHELKTPIAAIRANVQVLEQQIPGNRYLDHVVSETKRMEF
                     LIEDLLNLSRLDEKRSKVNFKKLNLSVLCQEVLLTYESLAYEEEKCLNDTIEDDVWIV
                     GEESQIKQILIILLDNAIRHSLSKSAIQFSLKQARRKAILTISNPSAIYSKEVMDNLF
                     ERFYQAKDDHADSLSFGLGLSIAKAIVERHKGRIRAYQEKDQLRLEVQLPIDGFWTNT
                     MIN"
     gene            5044..5166
                     /locus_tag="VBE34_000827"
     CDS             5044..5166
                     /locus_tag="VBE34_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEO7263556.1"
                     /translation="MKPYLSFIGRTLLYFGILLLLIYFFAYLGRGQGSFIYNEF"
     gene            5179..6714
                     /gene="dltA"
                     /locus_tag="VBE34_000828"
     CDS             5179..6714
                     /gene="dltA"
                     /locus_tag="VBE34_000828"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEO7263557.1"
                     /translation="MIHDMIKTIEHFAETQADFPVYDILGEVHTYGQLKVDSDSLAAH
                     IDSLGLVEKSPVLVFGGQEYEMLATFVALTKSGHAYIPVDQHSALDRIQAIMTVAQPS
                     LIISIGEFPLEVDNVPILDVSQVSAIFEEKTPYEVTHSVKGDDNYYIIFTSGTTGLPK
                     GVQISHDNLLSFTNWMISDDEFSVPERPQMLAQPPYSFDLSVMYWAPTLAMGGTLFAL
                     PKTVVNDFKKLFATINELPIQVWTSTPSFADMALLSNDFNSETLPQLTHFYFDGEELT
                     VKTAQKLRQRFPKARIVNAYGPTEATVALSAVAITDEMLETCKRLPIGYTKDDSPTYV
                     IDEEGHKLPNGEQGEIIIAGPAVSKGYLNNPEKTAEAFFQFEGLPAYHTGDLGSMTDE
                     GLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSQYVKSAVAVPRYNKDHKVQNLLAYIV
                     LKEGVRDDFERDLDLTKAIKEDLKDIMMDYMMPSKFIYREDLPLTPNGKIDIKGLMSE
                     VNK"
     gene            6711..7976
                     /gene="dltB"
                     /locus_tag="VBE34_000829"
     CDS             6711..7976
                     /gene="dltB"
                     /locus_tag="VBE34_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEO7263558.1"
                     /translation="MIQAFLEKLPHLDVYGNPQYFFYLILAVLPIYIGLFFKKRFALY
                     EIIFSLSFIVMMLTGSTFNQLKSLLAYVVGQSLLVFIYKAYRKRFNHTLVFYVTVCLS
                     IFPLFLVKLIPAISEDGHQSLFGFLGISYLTFRAVAMIIEMRDGVLKEFTLWEFLRFL
                     LFFPTFSSGPIDRFKRFNEDYINIPDRNELLDMLGQAIHYLMLGFLYKFILAYIFGSL
                     IMPPLKELALEQGGVFNWPTLGVMYAFGFDLFFDFAGYTMFALAISNLMGIKSPINFD
                     KPFKSRDLKEFWNRWHMSLSFWFRDFVFMRLVKLLVKNKVFKNRNVTSSVAYIINMLL
                     MGFWHGLTWYYIAYGLFHGIGLVINDAWVRKKKNINKERRLAKKPLLPENKWTYALGV
                     FITFNVVMFSFLIFSGFLDLLWFPQPHNK"
     gene            7991..8230
                     /gene="dltC"
                     /locus_tag="VBE34_000830"
     CDS             7991..8230
                     /gene="dltC"
                     /locus_tag="VBE34_000830"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEO7263559.1"
                     /translation="MDIKSEVLAIIDDLFMEDVSSMMDEDLFDAGVLDSMGTVELIVE
                     LESHFNIDIPIAEFGRNDWNTANKIVAGVTELCNA"
     gene            8223..9485
                     /gene="dltD"
                     /locus_tag="VBE34_000831"
     CDS             8223..9485
                     /gene="dltD"
                     /locus_tag="VBE34_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEO7263560.1"
                     /translation="MLKRLGKVFGPLVCALLLLVGLYFVFPVSQPHHLGKEKNSAVAL
                     TKAGFKSRVQKVRAFSDPKANFVPFFGSSEWLRFDAMHPSVLAEAYNRSYIPYLLGQK
                     GAASLTQYYGIQQIKGQIKNKKAIYVISPQWFVRKGANKGAFQNYFSNDQTIRFLQNQ
                     TGTTYDRYAARRLLKLYPEASMSDLIEKVADGQKLSNKDKQRLKFNDWVFEKTDAIFS
                     YLPLGKTYNQAIMPHVGKLPKAFSYNHLSRIASQDAKVATRSNQFGIDDRFYQTRIKK
                     HLKKLKGSQRHFNYTKSPEFNDLQLVLNEFSKQNTDVLFVIPPVNKKWTDYTGLDQKM
                     YQKSVEKIKHQLQSQGFNHIADLSRDGGKPYFMQDTIHLGWNGWLELDKHINPFLTEE
                     NSKPNYHINNKFLKKSWAKYTERPSDYK"
     gene            9660..10052
                     /locus_tag="VBE34_000832"
     CDS             9660..10052
                     /locus_tag="VBE34_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 domain-containing protein"
                     /protein_id="HEO7263561.1"
                     /translation="MKSIKLLSFIVIILLGLGFTVGGLKVTENNKQQELWEIANSKDA
                     KNAYQKWIYAEDEDAFKENAVIKSYDIDKESIKKNPMGGISVRLIINKDPNLYITCNL
                     DRDNQGHLVSQSSHQSPQLTHLLESRGH"
     gene            10052..11344
                     /locus_tag="VBE34_000833"
     CDS             10052..11344
                     /locus_tag="VBE34_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO7263562.1"
                     /translation="MEKVLTDLDRVNIAKQEYELGSQLDTLVKIMSQDKVLPIGKVAH
                     VQDGGKETGEQIYTITPNGTLDKPEDVKEVTVLFKGSTAPFGGDDWKTDWFKNDIPIA
                     SKLLLKKFGSQSVSHKQGTKQLEQSAHLLKEVMNKYPNAKISVYGHSLGSMNAQYAIA
                     ELDDNQIYRVQGAWIYNGPNLYSILTDKQKEQVDKIKGVVNNYVDSKDKIGIGYPKEG
                     SDNAVGIVKIVDSKAVSGGSWVANFTNQHIWGGYQYNPDGSLKLAEDKFAYKENYSVV
                     HTNVNQRMHAIKAKLSSFKSTGKEVSKNQAIFLDSQQAKVISSGLVTISKEAVEEVKK
                     VEQKAVAKAEELYQSLKVAPTGVTELSPQELEEAYAQSGVSYASIVGEAQTFFSNKTQ
                     AAESISHAYVELENQINKGVEAMLRQDSNLSGEFKQWH"
     gene            11335..11727
                     /locus_tag="VBE34_000834"
     CDS             11335..11727
                     /locus_tag="VBE34_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263563.1"
                     /translation="MALIDIIEKQLADTQRKISDLDDAYHHSCCQFEEKLDDLSVRKN
                     KIINMLQETYDAVEYDLRYSNDSSDMMTLNRILGSYHDDLEQAYHKEYYALSAQEEEY
                     RANYIRQRSEHELTFEELQREKKRELMK"
     gene            11737..12018
                     /locus_tag="VBE34_000835"
     CDS             11737..12018
                     /locus_tag="VBE34_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263564.1"
                     /translation="MSLMWDVLTGKESVRTLKKQLDKELIHSVYAPRKRSKFHDIITS
                     HHKEIDEVAINTKTAIHKVANQLDEAIKGEFRSKVEEAIESNQQKYNHL"
     gene            12200..12982
                     /locus_tag="VBE34_000836"
     CDS             12200..12982
                     /locus_tag="VBE34_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEO7263565.1"
                     /translation="MIKIFDTHTHLNVENFEGKIDEEINLASELGVTKMNVVGFDQDT
                     ISKSLELSSQYAQVYSTIGWHPTEAGSYDDNIESMIISHLENPKVIALGEIGLDYYWM
                     EDPKDIQIEVFKRQIELSKEYNLPFVVHTRDALEDTYEVIKESGVGPFGGIMHSFSGS
                     LEMAQKFIDLGMMISFSGVVTFKKALDVQEAARELPLDKILVETDAPYLAPVPKRGRE
                     NKTAYTRYVVEKIAELRGITVEEVAEATYQNAVRIFRLDEKN"
     gene            12969..13529
                     /gene="rnmV"
                     /locus_tag="VBE34_000837"
     CDS             12969..13529
                     /gene="rnmV"
                     /locus_tag="VBE34_000837"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEO7263566.1"
                     /translation="MKKIDIQEVIVVEGKDDTANLRRFYNVDTYETRGSAIDEDDLER
                     IERLHNLRGVIVFTDPDYNGERIRKIIMNAIPTVRHAFLNRDEAKPGSKTKGRSLGVE
                     HASFEDLQKALSKVTQHFDDEDHFDITQADLIRWGFITASDSRKRREYLGNQLRIGYS
                     NGKQLLKRLRLFGVTKAEVEECMEGY"
     gene            13548..14039
                     /locus_tag="VBE34_000838"
     CDS             13548..14039
                     /locus_tag="VBE34_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263567.1"
                     /translation="MEYNVTEALTRAGVDLAMLAVKGTATMVTSKIQSIKDVKDAATL
                     RATYDDLINELLAEREQAIRIAQSYKEEYEKINIDDEDIEYLSNTLEQGISLLYNFSG
                     LNEEQERTMKQLVTLLNKDTLKTMQLIGFNYKQAIGEPLTEACATAIKNKFITQNSKG
                     KRK"
     gene            14119..14991
                     /gene="rsmA"
                     /locus_tag="VBE34_000839"
     CDS             14119..14991
                     /gene="rsmA"
                     /locus_tag="VBE34_000839"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEO7263568.1"
                     /translation="MRIADKTVTRAILERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DKGVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLIPILADTLARFDNVQVVNQDIL
                     KADLQTQIQAFKNPDLPIKVVANLPYYITTPILMHLIESKIPFAEFVVMIQKEVADRI
                     SAMPNTKAYGSLSIAVQYYMTAKVSFIVPRTVFVPAPNVDSAILKMVCRDQPVVSVQD
                     EDFFFRVSKVAFVHRRKTLWNNLTSHFGKSEDTKAKLEKALEIAKIKPSIRGEALSIP
                     DFASLADALKEVGI"
     gene            14995..15846
                     /locus_tag="VBE34_000840"
     CDS             14995..15846
                     /locus_tag="VBE34_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO7263569.1"
                     /translation="MLVDKKWRFEDSASYFACPKCQNPLIKESNSLKCSDNHCFDLSK
                     FGYVNLLGGKKVDEHYDKKSFENRQLVLENGYYNHILEAISKVLENNSQFHSVLDIGC
                     GEGFYSRQLVNKHEKTFLAFDISKDSIQLAAKSDQSRLVKWFVSDLANLPIQDSSIDI
                     ILDIFSPANYKEFRRVLSDDGILVKVVPVAEHVQELREKASQYLKQKDYSNQKILDHF
                     RENFEIISEQKVVQSYNCSQQERQAFIDMTPLLFSVDKTTIDWASISEITVGALIVIG
                     KKRSVSK"
     gene            16002..16874
                     /gene="rsgA"
                     /locus_tag="VBE34_000841"
     CDS             16002..16874
                     /gene="rsgA"
                     /locus_tag="VBE34_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEO7263570.1"
                     /translation="MQGRIVKSLAGFYYVESDGVVYQTRARGNFRKKGQIPYVGDWVE
                     FSSQDQSEGYILSIEERKNSLVRPPIVNIDQAVVIMSAKEPDFNANLLDRFLVLLEYK
                     MIQPIIYISKLDLLDDLVVIDDIRELYQNIGYVFCYSQEELLPLLANKVTVFMGQTGV
                     GKSTLLNKIAPELKLETGEISGSLGRGRHTTRAVSFYNVHKGKIADTPGFSSLDYEVD
                     NAEDLNESFPELRRLSHFCKFRSCTHTHEPKCAVKEALTQGQLWQVRYDNYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            16881..17543
                     /locus_tag="VBE34_000842"
     CDS             16881..17543
                     /locus_tag="VBE34_000842"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEO7263571.1"
                     /translation="MSTNKIAPSILAADYANFANELKRIEETTAEYVHIDIMDGQFVP
                     NISFGADVVSSMRKHSKLVFDCHLMVVDPERYIEAFAQAGADIMTIHVEATKHIHGAL
                     QKIKEAGMKAGVVINPGTPVESLIPILDLVDQILIMTVNPGFGGQAFIPEMMSKVKTV
                     AAWRKEYGHHYDIEVDGGIDNTTIKAAAEAGANVFVAGSYLFKASDLPAQVETLRVAL
                     DD"
     gene            17536..18168
                     /locus_tag="VBE34_000843"
     CDS             17536..18168
                     /locus_tag="VBE34_000843"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEO7263572.1"
                     /translation="MTKIALFAGGDLTYFEYDFDYFVGIDRGSLFLLKNGLSLDMAVG
                     DFDSITEDELLYIKHYCSNIVSASAEKNDTDTELALKTIFKEFPEAQVTVFGAFGGRI
                     DHMMSNIFLPSDRDLEPFMSQIRLKDEQNIVTYLPSGKNQVSRIEGMSYVSFMPESES
                     TLQISGAKYELNKSNYFKKKMYSSNEFMTSPIEVELKDGYLIIIYSKDRG"
     gene            18170..19444
                     /locus_tag="VBE34_000844"
     CDS             18170..19444
                     /locus_tag="VBE34_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEO7263573.1"
                     /translation="MLVIILIIVLASLTVTIISYPKMTELTKSVEKQLEDNADNLSDQ
                     LTYQIEVAQKDQILTLTNQLNRMQQEIYQLLTDMRTELNQHLTESRDRSDKRLELINS
                     NLSQSVQKMQDSNEKRLDQMRQTVEEKLEKTLQTRLQTSFETVSRQLESVNQGLGEMK
                     TVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTVSQYEREFPTVSGSSERVEYAI
                     KLPGNGQGDYIYLPIDSKFPLEDYYRLEDAYELGDKVQIELYRKSLLASIRKFAKDIN
                     NKYLNPPETTNFGIMFLPTEGLYSEVVRNATFFDSLRRDENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNANDISKILGNVKVEFGKFGGMLSKAQKQLNTASKSIDSLLTTRTNAI
                     IRVLNTVEEHQDQATTSLLNLPITEEEEINEN"
     gene            19434..20375
                     /locus_tag="VBE34_000845"
     CDS             19434..20375
                     /locus_tag="VBE34_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEO7263574.1"
                     /translation="MKINQMKKDELFEGFYLIKKAEVRKTRAGKDFIAFTFQDDTGEI
                     SGNMWDAQTYNVEEFVAGKIVHMKGRREVYNGTPQVNQITLRNIKDGEPNDPRDFKEK
                     PPINVDNVREYMEQMLFKIENATWQRVVRALYRKYNKEFFTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPELNKSLMFAGIMLHDLAKVIELSGPDNTEYTIRGNLIGHIS
                     LIDEELTKILAELNIDDTKEEVTVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALNRVNEGEMTNRIFAMDNRSFYKPNIK"
     gene            20472..21284
                     /gene="purR"
                     /locus_tag="VBE34_000846"
     CDS             20472..21284
                     /gene="purR"
                     /locus_tag="VBE34_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEO7263575.1"
                     /translation="MKLRRSERMVVISNYLINNPYTLTSLNTFASKYGAAKSSISEDI
                     AIIKKAFEQAQIGDIKTVTGASGGVIFTPTIAEAEAKEIVEELRQRLSENDRILPGGY
                     IYLSDLLSTPKMLQSIGRIIANAYRGQKIDAVMTVATKGVPLANAVANVLDVPFVIVR
                     RDLKITEGSTVSVNYASGSSGRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTVSGMISL
                     LSEFDSTLVGVAVFAENAQEQREKMAYKSLLRVSEIDVKNNRVSVEAGNIFD"
     gene            21503..21916
                     /gene="rpsL"
                     /locus_tag="VBE34_000847"
     CDS             21503..21916
                     /gene="rpsL"
                     /locus_tag="VBE34_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEO7263576.1"
                     /translation="MPTINQLVRKPRKSKVEKSDSPALNIGYNSHRKVHTKLSAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            21938..22408
                     /gene="rpsG"
                     /locus_tag="VBE34_000848"
     CDS             21938..22408
                     /gene="rpsG"
                     /locus_tag="VBE34_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEO7263577.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFEAIKEATGTDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            22563..24641
                     /gene="fusA"
                     /locus_tag="VBE34_000849"
     CDS             22563..24641
                     /gene="fusA"
                     /locus_tag="VBE34_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEO7263578.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQSLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYVDQANEYREKLVEAV
                     ADTDEDLMMKYLEGEEITNEELMAAIRKATINVEFYPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDEEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     SKVILESIEVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEARGNTQVVRAFVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNSGE"
     gene            24845..25855
                     /gene="gap"
                     /locus_tag="VBE34_000850"
     CDS             24845..25855
                     /gene="gap"
                     /locus_tag="VBE34_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEO7263579.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGQFVKVSAEREPANIDWATDGVEIVLEATGFFAS
                     KEKAEQHIHENGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALQDNFGVKQGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVATLEKDVTVEEVNAAMKAAANDSYGYTED
                     PIVSSDIVGISYGSLFDATQTKVQTVDGNQLVKVVSWYDNEMSYTSQLVRTLEYFAKI
                     AK"
     gene            26064..26933
                     /locus_tag="VBE34_000851"
     CDS             26064..26933
                     /locus_tag="VBE34_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEO7263580.1"
                     /translation="MKTTKVFSVLSLSVAVFMMTACQSNHSTKKADMSPDKVEQKTKF
                     KNISLSSNKLLAKENTMSVLWYQNSAEAKALYLQGYNVAKMKLDDWLQKPSEKPYSII
                     LDLDETVLDNSPYQAKNIKDGSSFTPESWDKWVQKKSAKAVAGAKEFLKYANEKGIKI
                     YYVSDRTDAQVDATKENLEKEGIPVQGKDHLLFLKKGMKSKESRRQAVQKDTNLIMLF
                     GDNLVDFADFSKSSSTDREQLLTKLQSEFGSKFIVFPNPMYGSWESAIYQGKHLDVQK
                     QLKERQKMLHSYD"
     gene            27068..28264
                     /locus_tag="VBE34_000852"
     CDS             27068..28264
                     /locus_tag="VBE34_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEO7263581.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGAKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
BASE COUNT         9545 a         4489 c         5671 g         8655 t
ORIGIN      
        1 aatcatggat ttaattacaa gacttaaagt tgaacgagat ggcagagttt atggtggcat
       61 ttatgatatt actcagaagc gttttgcatt taattcaaat aagattgaag gcagtcgttt
      121 aactgaagag caaacgagtt tcatttatga aacaaaaaca atcgcaaata ttggtggaac
      181 aggtatcaaa attgatgatc ttgttgaaac tactaatcat tttaaatgtt ttgattatat
      241 tattaataca gttgatgagc agctaacaga agactacgtt aaaaaactac atagcatttt
      301 aaaagctgga acaagctcag aatataatga atatgtccca gttggacgtt ataaggtatt
      361 tgaaaacgaa gtaggacaga ttgctacagc tgctgttgat caagttgaag aggaaatgta
      421 ctcactactt ttgggttata atttcaaaaa agaaaaagat ttagcttatc tagcatcttt
      481 ctatgcacgt tttgagcaaa ttcatccgtt cgctgatggt aatggtcgtg ttggacgttt
      541 gatcctctac aaggaatgtt taaaagaagg aataactcca ttcattgtag atgatacaag
      601 aaaagcagat tattacaatg cactagaaca atttcaagtt tatgataacc atcaaccttt
      661 gattgattat tttgcgattg aacaaaaatt ttatgcaaat taccttaaaa acaaaggatt
      721 tgtgggaaaa ataaacgata gcaaagacag agatgttatc aataaaaaaa gtaaaagtgc
      781 tgatgatagc cataaaaggc caaacaaaac aaaaggtttt tcgttataaa tatagttata
      841 tttaaaaatc ttcatttcca tgaggagttt tttgtgtgat agtttatcca aaattaggta
      901 aaatacctgt atgacaggta tgagatttga tgaaaaggtt tttgttgttg aattagatga
      961 taaacgtaaa tacgactttt taaaaagagt taaaacgtat gatgcaaata tcgcctcagc
     1021 aatgcggata gctggataca aacgtatcaa tcaaacagag agaacggttg cttttacttt
     1081 tggagaagta acattctcta ggtcacgttg gcgaaaagga aataaaacac gttgtcctat
     1141 agatgaatgg ttagggctta aaaagtatat gcgtttttct cctgaattga tttgccatat
     1201 agctaaaatg gctacagtga tgtcgtatcg tcaagtgtgt cattttgtta aagaatttta
     1261 tcatcttgag attactaagg atactgtttt aaaagctgtt aagcgagctg gggatttgct
     1321 gtctgaaaaa gaaaagtttc gcttttggct agaggaagaa cctaccaaga aaattaaacc
     1381 aaaaatcctt tatattgaag gagatggcgt gatggtgaaa tcatcagatt ctgctagaga
     1441 tgacaaaaga aatgttgatt taacgcactt tcttgttcat actggttcta aaaaaattgc
     1501 tcaaaatcgt tatgtcctag aaaataaaca tgagataatc catacaaatt acgaaaaggc
     1561 aaaagaagaa ttactagact atttgtataa tcattacgag atcacagatg agacgatatt
     1621 aatcactaat tctgataatg gtaaaggcta tactccaaaa gtttttcaag aaataaaaaa
     1681 agcgcttggt atcaaaagac acgagcactt ttgggatgct tttcacttac atgaaaagat
     1741 aaaaatattt tttaaaaaat atccagctga attaaaagaa ttagcttttg aggcaattaa
     1801 tactcacaaa agagagttaa tgaaaacagt tcttgatacc acagagtctt taatcgatgg
     1861 tgaaggtgat gaatactata attattttaa ttttcgccgt aaaattctta ataatttcaa
     1921 atatacgaag cctgctaaat taagaaattt gtcttctaaa ggaataggtg tcatggaaag
     1981 tcagcatcga aaaataacct atcgaatgaa acacagaggt atgtattggt ctttgaaagg
     2041 ggcatataca atgtccaggt taatcatttt agagagaatc aatcttgttg atgatttatt
     2101 ttttggtgat tggcgaaaag agtatcaata ctatcaaaat caacgtttta gcgttggtta
     2161 ccttaaggag ataaaagcag agacgattat aaaaacaaga aaaatatttc agcgtgctgg
     2221 gaagtttact tcacttgatc gtcaaaaatt taagtattaa agactttaaa attttttgat
     2281 tttaaggctt ttttcagctg ttttcgtttg catttttctg acaattttgt tataatatca
     2341 ttgtagaaaa tcaaaaaagg agcagaagcg gtaacttcta ctccaccgat taagacaaat
     2401 ctacactttg cttaatcagt ctgaagttat tgtaacacga aaaagccaat aaagtcaaga
     2461 ctgaagcaag gaaacctttg tttcggtatt tttttgaaaa aataattgag aaatctcttg
     2521 atttataggc tggtgagtat tctcaaaaaa ttagacactt acaacttgaa agtatttctt
     2581 gacaagaggc ctaaaattgt tataaaatag tatggtatgt ttagtaactg atcaaaatag
     2641 ccggctaaac gaatacgaaa atctatcagg aggtattcaa cgtgaaacgt acttatcaac
     2701 caagtaaaat ccgtcgccaa cgtaaacacg gattccgtca ccgtatgtca actaaaaacg
     2761 gacgtcgcgt gcttgctagc cgccgtcgta aaggacgtaa agtgttatca gcttaatata
     2821 gatagcaaaa taatatcaag ccaacaggta ctcgagtctg gtggcttttt tggtaccttc
     2881 ataacgataa aggtttttgt gagagtagtt tgagtatatt atagataaag ttaatgaaaa
     2941 gtgagtttat aatgcggaaa ttcgtagaca aatagcccct atttttgtta gaatatgagg
     3001 agatttctta ttgagaaata tctttatagc gagtgatatt ttatttttta gacgcttgta
     3061 ttattgaagt agtttagtga cttaggaaat acttttatta atatcgtttt ttcaaggtta
     3121 tttcaatttt gctagtttat actataatat ataaggtaag gtgactagaa aatgagactt
     3181 cttgtagttg aggatgaaaa aagtatagca gaggctattc aggctttatt ggcagataaa
     3241 ggttattctg ttgatttagc atttgatgga gatgatgggt tagaatacat tttgacaggt
     3301 ctctatgatt tagtcctgtt ggatattatg ttacccaaac gctcaggatt atctgtttta
     3361 aaaagagtga gggaagctgg tttagaaacc ccaataatct ttttaactgc aaaatcacag
     3421 acttatgata aagttaatgg tttagattta ggggcagatg attatatcac aaaacccttt
     3481 gaagcagatg aattgctagc tagaattagg ctgagaacca gacaatcttc tctaatcaga
     3541 gcaaatcaat tgcggcttgg aaatataagg cttaacacag attcacatga gctagaatct
     3601 aaagaaagta gtgtaaaatt atccaataaa gaatttctct tgatggaagt ttttatgcga
     3661 aatgcaaagc agattattcc taaaaatcag ttgatttcta aggtttggag accttcagac
     3721 aattcagaat ataaccagtt agaagtattt atttcttttt tgcgaaaaaa attgcgcttt
     3781 ttaaaagcag acattgaaat aataaccact aagggctttg gatattcctt ggaggagagg
     3841 acataatgtt tagtgattta cgcaaaaaat ttgtcttttt gaccatgtca atccttattg
     3901 tcgttgttct ctttctattt gcagttagta atcgctacaa tcaatattgg gatgaatatg
     3961 atgcatatag gattgttaaa ttagttgcca aaaatgatta tttaggaata ccaggcgatg
     4021 aaccgattgc tttggtgact atagataacc aaaagatggt taagattcaa agcaataata
     4081 ctgatttgac aaatgatgtt atcgaaaaat ctagtttgaa gctccttgag caaggaaaga
     4141 agtcacgaaa atggaagtct tttatatatt ccataaaaga atataaagat aaaacctaca
     4201 ccatagctat tatggatttg gctagttatg aagtccctta tgcaaggcgt tttctaattc
     4261 ttgtttttac tatttttgga ttttgtttat tagcagctgt aagtctttat ctttctcgat
     4321 ttattgtagg acctgtagag acagaaatga ctcgcgaaaa acagttcgtt tcagatgcga
     4381 gtcatgagct gaagacccct attgcagcta tacgagccaa cgttcaggtt ctggaacagc
     4441 aaattccagg gaatcgttat ctggatcatg ttgtttcgga gactaagcga atggagtttt
     4501 taattgaaga tttgcttaat ttgtctagac ttgatgaaaa acgctccaag gtaaatttca
     4561 aaaagttaaa cttatctgtc ttatgtcaag aagtacttct aacatatgaa agtttggcat
     4621 atgaagaaga aaagtgttta aatgatacga ttgaagatga tgtctggatt gttggagaag
     4681 agagtcagat taagcaaatt ttaataattc ttttggataa tgctattagg cattctttgt
     4741 caaaatcagc aatccagttt agtcttaaac aagctcgaag aaaagctatt ctgacaatat
     4801 caaatccaag tgctatctat tccaaagagg ttatggataa tctctttgag cgtttttatc
     4861 aagctaaaga tgatcatgca gattctctca gttttggtct aggcttatct attgctaaag
     4921 caatcgtgga acgccataag gggagaatta gagcatatca agaaaaagac caattacgtc
     4981 ttgaagtcca gttaccaatt gatggatttt ggaccaatac catgattaat taaaggaaga
     5041 atgatgaaac catatttatc ttttattggt agaacgttat tatacttcgg tattttattg
     5101 ttactaattt acttttttgc ataccttggt cgcggacaag gcagttttat ttataatgaa
     5161 ttctaggaga tggtacaaat gatacatgat atgattaaaa caattgagca ttttgctgag
     5221 acacaagctg attttccagt gtatgatatt ttaggggaag tccatactta tggacaactt
     5281 aaagtagact ctgactctct agctgctcat attgatagcc taggccttgt tgaaaaatca
     5341 cctgtcttag tattcggtgg tcaagaatat gaaatgttgg cgacatttgt tgctttaaca
     5401 aagtcagggc atgcttatat accggttgac caacactctg ctttggatag aatacaggct
     5461 attatgacag ttgctcaacc aagccttatc atttcaattg gtgaatttcc tcttgaagtt
     5521 gataatgtcc caatcctaga cgtttctcaa gtttcagcta tttttgaaga aaagactcct
     5581 tatgaggtaa cacattctgt taaaggtgat gataattact atattatttt cacttcaggg
     5641 actactggtt taccaaaagg tgtgcaaatt tcacatgaca atttattgag ctttacaaat
     5701 tggatgattt ctgatgatga gttttcagtt cctgaaagac cgcaaatgtt ggctcaaccg
     5761 ccatattcat ttgacttatc agttatgtat tgggcaccaa cactagctat gggaggcacc
     5821 ctgtttgccc taccaaaaac agtagttaat gatttcaaaa aactattcgc taccattaat
     5881 gaattgccaa tacaggtttg gacttcgaca ccatcatttg ctgatatggc gctactatct
     5941 aacgatttca attcagagac cttgccacag ttaacacatt tttattttga tggggaagag
     6001 ttaactgtca agactgcaca aaaacttcgt caacgttttc caaaagctcg tatcgttaat
     6061 gcatatgggc caacagaagc aacagttgct ctatccgcag tagcaattac tgatgaaatg
     6121 ttagaaacat gcaaacgcct tccaattggt tacactaaag atgactcacc aacgtatgtg
     6181 attgatgaag aaggtcataa attaccaaac ggagagcaag gtgaaatcat tattgctgga
     6241 ccagcagtat caaaaggcta tcttaacaat ccagaaaaga cagctgaggc atttttccaa
     6301 ttcgaaggtc tacctgctta ccataccggt gacttaggaa gtatgaccga tgaaggtctt
     6361 ctgctttacg gtgggcgtat ggatttccaa attaaattta acggctatcg tattgaatta
     6421 gaagatgttt ctcaaaactt aaacaaatcg cagtatgtaa aatcagcagt agcagtgcca
     6481 cgttataaca aggatcataa agttcaaaac ttattagcct atattgtctt aaaagaaggt
     6541 gtaagagatg attttgaacg tgatttggat ttgacaaaag caattaagga agacttaaag
     6601 gacattatga tggattacat gatgccatct aaatttatct atcgagagga tttacctttg
     6661 acaccaaatg ggaaaattga tatcaaaggt cttatgagcg aggtaaacaa gtgatacagg
     6721 catttttaga aaaacttcct cacttggatg tttatgggaa tccacaatat ttcttctacc
     6781 ttatcttagc tgtcctacca atttacatcg gcttattctt taagaagcgt tttgccttat
     6841 atgagattat ttttagtcta agttttattg taatgatgtt gactggtagt acttttaatc
     6901 aattgaagtc actattggca tacgttgtcg gacagtctct gctagttttt atctataaag
     6961 cttaccggaa acgatttaat catactttgg tcttttatgt aacggtttgt ttatctattt
     7021 ttccgctatt tttggtaaaa ttaattccag ctatatctga ggatgggcat cagtcacttt
     7081 ttgggtttct aggaatttct taccttactt ttagagctgt tgctatgatt attgaaatga
     7141 gagacggtgt cttgaaagaa tttactttat gggaattctt aagattttta ctcttctttc
     7201 caactttctc aagtggacca attgatcgtt ttaaacgatt caatgaggat tacattaata
     7261 tcccagatcg aaacgaactc ctagatatgt taggtcaagc gattcattat ttgatgttag
     7321 gttttctcta taagtttatt ttagcctata tttttggaag tctgattatg cctcctctaa
     7381 aagaattagc gctagaacag ggtggtgtgt ttaattggcc aacacttggg gttatgtatg
     7441 cctttggttt tgatttgttc tttgattttg caggttacac aatgtttgcg ttggctattt
     7501 ctaacctaat ggggattaag tctccgatta actttgacaa acctttcaaa tcacgcgacc
     7561 taaaagaatt ttggaataga tggcatatga gcctttcttt ctggtttaga gactttgttt
     7621 tcatgaggct tgttaagctt ttagttaaaa ataaagtttt taaaaaccgt aatgttactt
     7681 caagtgtagc ttatattatc aatatgcttc ttatgggatt ctggcatggg ttaacttggt
     7741 actatatagc ctatggtctc tttcatggga ttggcctagt tattaatgac gcttgggtac
     7801 gtaagaagaa aaatattaat aaagaaagaa gattggctaa aaaaccactt ttaccagaaa
     7861 acaaatggac ttatgctttg ggtgtcttca tcacctttaa tgtagttatg ttttctttct
     7921 tgattttttc aggattttta gatcttttgt ggttcccaca accgcataac aaataataag
     7981 gagtattata atggatatta aatcagaagt attagcaatt attgatgacc tatttatgga
     8041 ggatgtttct tctatgatgg atgaagactt atttgacgca ggagttctag acagcatggg
     8101 tactgttgaa ctaattgttg agttggaaag ccactttaat attgatattc cgattgcgga
     8161 atttggtcgt aatgactgga acacagctaa taaaattgtt gctggagtaa cggagctctg
     8221 caatgcttaa acgtctaggt aaagtatttg gacctctagt ttgtgcttta ctattgttgg
     8281 taggattata ttttgttttt cctgtttctc agcctcatca tttaggtaag gaaaaaaaca
     8341 gtgcagtagc gttgacgaag gcaggtttta aaagcagagt tcaaaaagtt agagctttca
     8401 gtgatcctaa agccaatttt gtccctttct ttggttcaag tgagtggcta agatttgacg
     8461 caatgcatcc atcagtttta gcagaggctt acaataggtc ttatatccca tatcttttag
     8521 gtcaaaaagg ggcggcttct ctgacacaat actatggcat tcaacagatt aaaggacaaa
     8581 tcaaaaataa aaaagctatc tatgttattt ctccgcaatg gtttgttcgc aagggagcca
     8641 acaaaggtgc ttttcaaaac tatttcagca acgatcaaac cattcgattt ttgcaaaatc
     8701 aaacagggac aacctacgat aggtatgctg ctcgtcgatt gttaaaatta tatcctgaag
     8761 cttctatgtc agatttgata gaaaaagttg cagatggcca aaaactatca aataaagaca
     8821 aacaaagact aaagtttaat gattgggtat ttgagaagac agatgctatt tttagctatc
     8881 taccactagg aaaaacttat aatcaggcaa taatgcctca tgttggtaaa ttaccgaaag
     8941 cattctcata taatcattta tcgcgtattg catcacaaga tgctaaagta gcaacgagat
     9001 caaatcaatt tggtattgat gatcgctttt accaaacaag aattaaaaag cacttaaaaa
     9061 aattgaaagg gtcacaacga catttcaatt atactaagtc accagaattt aatgatttac
     9121 agttggttct taatgaattc tcaaaacaaa atacagatgt cctttttgtc ataccaccag
     9181 taaataaaaa gtggacagac tacacagggc ttgatcaaaa aatgtatcaa aaatctgtag
     9241 aaaaaataaa acaccaactt caaagtcaag gtttcaatca tatcgctgac ctttctcgag
     9301 atggaggtaa gccatacttt atgcaagata caatccattt aggttggaat ggttggttag
     9361 agctagataa gcatatcaat ccatttttaa cagaggaaaa cagcaagccg aattatcaca
     9421 ttaataataa atttttgaag aaatcttggg caaaatatac agaacgtcca agtgattaca
     9481 agtaaatagt agagtcagat gatttatagc attagagcta ttaatcatct tttttaatat
     9541 ctttgtattt ggtaatccta agataactaa tccatgtttt atagtttttc atttataatg
     9601 agaataaatt tatgataaac tagatatcaa atgagtgtta tatgataaaa ggaggagaga
     9661 tgaagtcaat aaaattactt agttttattg taataatcct tttagggctt ggtttcactg
     9721 taggaggttt aaaagtgact gaaaacaata aacaacaaga attatgggaa atagctaata
     9781 gcaaagacgc taaaaatgct tatcaaaagt ggatttatgc agaagatgaa gatgctttca
     9841 aagagaatgc tgttattaag tcgtatgata ttgacaaaga aagtatcaaa aaaaatccta
     9901 tgggagggat ttcggtaagg ttaatcatta ataaagatcc taacttatat attacttgta
     9961 atttggatag ggataatcaa gggcatttgg tcagtcaaag tagccatcaa tctccccagt
    10021 taactcattt attggaaagt agaggtcatt aatggaaaaa gtcttgactg atttagatag
    10081 ggtaaatata gcgaagcaag agtatgaatt aggtagccaa cttgatacac tagtcaaaat
    10141 aatgagccaa gataaggtac tccccatcgg taaagttgct cacgttcaag atggaggcaa
    10201 agaaaccggg gaacaaattt ataccatcac tccaaatgga actttagaca agccagagga
    10261 tgttaaggaa gttacggttt tatttaaggg gtcaacagca ccgtttggag gggatgattg
    10321 gaaaacagac tggtttaaaa atgatattcc aatagctagt aaactattat taaagaaatt
    10381 tggttcacaa agtgtatctc ataaacaagg tacaaagcag ttagaacaat cagctcatct
    10441 gctaaaagaa gttatgaata aatatcctaa cgcaaaaatt tcggtctacg gacactcgtt
    10501 agggtcaatg aatgcacagt atgctattgc agaattggat gataatcaaa tttaccgagt
    10561 tcaaggagct tggatatata acggtccaaa cctttatagt attttgacag ataaacaaaa
    10621 agaacaggtt gataaaataa aaggggtggt caataattac gttgattcca aggataaaat
    10681 tggaataggt tatcctaaag aaggaagcga taatgctgtc ggaattgtta aaatagttga
    10741 cagtaaagct gtttcaggag gtagctgggt tgctaatttt accaatcagc atatttgggg
    10801 aggctaccag tacaatccag atggttcttt aaaattagca gaagataaat ttgcttacaa
    10861 agaaaactat tcagtagttc atacgaatgt gaatcaaaga atgcatgcta ttaaagcgaa
    10921 acttagtagc tttaaaagta cgggaaaaga agttagtaag aatcaagcta ttttcctaga
    10981 tagtcaacaa gcaaaagtga tctctagtgg tttggtaacg atatcaaaag aagcagttga
    11041 agaagttaaa aaagttgagc aaaaagcagt tgctaaagca gaagagcttt atcaatcttt
    11101 aaaagtagct cctacaggag ttactgaact aagtcctcaa gaattggaag aagcgtatgc
    11161 tcagtcaggt gttagctatg cgagcattgt cggagaagct caaacctttt tctcaaataa
    11221 aactcaagcg gcagaaagca tttctcacgc ttacgttgaa ttagaaaacc aaattaataa
    11281 aggagttgaa gctatgttgc gacaagatag taacttgtca ggggagttta aacaatggca
    11341 ctgattgata tcattgaaaa acaattagct gatactcaga gaaaaatatc agatttagat
    11401 gatgcctatc accatagttg ttgccagttt gaagaaaaat tagacgatct ctcagtaaga
    11461 aaaaataaga ttattaatat gcttcaagaa acttacgacg ctgttgaata tgatttgaga
    11521 tatagtaatg atagctcaga tatgatgacg ctcaatcgta ttttaggttc ttatcatgac
    11581 gacttagagc aagcttacca taaagagtac tatgccttgt ccgctcaaga agaagaatat
    11641 agagctaact atattcggca acgttcagaa catgaactaa cctttgaaga gttgcaaagg
    11701 gaaaagaaaa gagaattaat gaagtaggag aaattattga gtttaatgtg ggatgtcctg
    11761 actggtaagg agtcagtaag gactttaaaa aaacaattgg ataaagaatt gatacatagc
    11821 gtgtacgcgc cacgcaagag atctaagttt cacgatatta ttacgtcgca tcataaagaa
    11881 atagacgaag tagccatcaa cacaaagaca gctattcata aagttgctaa tcaattggac
    11941 gaggcaataa aaggagaatt tcgttcaaag gttgaggagg ctattgaaag caatcagcaa
    12001 aaatataatc atttatagtc tagtttctct accattagct agacacttgt agaggatttt
    12061 tacagcaagt attcaaaagt cagagcaaaa atgttttgac ttttttgatt tactctttct
    12121 gatcagaatg cttcagtctt atgttataat aataaagatt aatgcttgta gctacgataa
    12181 gcaaagaaag aacgatgata tgataaaaat atttgatacc catacccacc ttaacgtaga
    12241 aaattttgaa ggaaagatag acgaggaaat taatttagct agcgaactcg gtgttacaaa
    12301 aatgaatgtt gtcggctttg accaagatac catttcaaaa tctttggaat tatcttccca
    12361 atatgcacag gtatatagca caataggatg gcatccaact gaagctggtt cttatgatga
    12421 taatattgag tcaatgatta tcagtcattt agaaaatccc aaggttattg cacttggtga
    12481 aattggtctg gattattatt ggatggagga tcccaaagat attcagatcg aggtgtttaa
    12541 gcgtcaaatt gaactttcaa aagagtataa ccttcctttt gtcgttcata cacgtgatgc
    12601 gttagaagac acttatgaag tcattaagga atctggagtt gggccttttg gtggtataat
    12661 gcactctttt tctggttctt tggaaatggc tcagaaattt attgatttag ggatgatgat
    12721 ttccttttct ggtgttgtga cctttaagaa ggcattagat gtacaagaag ctgcacgaga
    12781 gttgccatta gataagatat tagtggaaac agatgctcct tatttggctc ctgtacctaa
    12841 aagaggtcga gaaaacaaaa ccgcttatac gagatatgtc gttgaaaaaa tagcagagct
    12901 acgaggcata actgtggaag aggtagcgga ggctacatac caaaatgcag taaggatatt
    12961 tagacttgat gaaaaaaatt gatattcaag aagtgatcgt tgtcgaaggg aaagatgata
    13021 ctgctaatct gcgacgcttt tacaatgttg atacctatga aactagaggt tcagcaattg
    13081 atgaagatga tttagagcgt atcgaacgtt tgcacaattt gcggggagtt atcgtcttta
    13141 cagacccaga ttataatggc gagcgtatcc gtaaaatcat catgaatgcc attccgacag
    13201 tccgccatgc cttcctcaat cgagacgagg caaagccagg ctcaaagacc aagggacgat
    13261 ctctgggtgt cgagcatgcc agctttgaag atttgcagaa ggcactctca aaagtgactc
    13321 agcatttcga tgatgaagat catttcgata tcacccaagc agacctcatt cgttggggat
    13381 tcattactgc tagcgatagc cgaaaacgcc gtgagtatct tgggaatcaa ctccgtatcg
    13441 gctactcaaa tggtaaacaa ctcctcaaac gcctcagact cttcggagtg accaaggcag
    13501 aagttgaaga gtgtatggag gggtattaat agaaaaggag aaaaatagtg gagtataatg
    13561 taacggaagc attgactaga gctggggtag atttagcaat gcttgcagtc aaagggactg
    13621 caactatggt aaccagtaaa attcaatcaa taaaggatgt aaaagatgct gcaacattga
    13681 gagctactta tgatgattta attaacgaat tattagctga acgggagcaa gcaattcgca
    13741 ttgcacagtc atataaagaa gagtatgaaa aaattaatat agatgatgag gatattgaat
    13801 atctatccaa tactctcgag caaggaatct cattgttata taatttttct ggtctaaatg
    13861 aggaacagga acgaacaatg aaacaattgg ttacattatt gaataaagat actttaaaaa
    13921 caatgcagtt gattggattt aattataagc aagcaatagg agaaccacta accgaagctt
    13981 gtgcaactgc aattaaaaat aagtttataa ctcagaatag taaaggcaaa aggaagtagt
    14041 ggtataatcc agttgtaata tcacaaatag cctcaaatgt tatttcaaaa atgatcaata
    14101 ttaagaaata gaggaattat gagaatcgca gataaaaccg ttacccgcgc tattttggag
    14161 cgtcatgggt ttacatttaa gaaatcattc ggtcagaact ttttgaccga tactaatatt
    14221 ttacagaaga ttgttgacac ggcagagatt gacaagggtg tcaacgtgat tgagattggt
    14281 cctggtatcg gggctttgac agagtttttg gctgagaatg cagctgaggt tatggctttt
    14341 gagattgatg atcgtctcat tcctatcctt gctgatacgc ttgcgcgttt tgacaatgtc
    14401 caagtggtca atcaggatat cctcaaggct gatttgcaga cccagattca ggctttcaag
    14461 aatcctgatc tgcctatcaa ggtggtcgca aacctacctt actacatcac aacaccaatc
    14521 cttatgcact tgattgagag caagattcct tttgcggagt ttgtggtcat gatacaaaaa
    14581 gaagtggcag atcgtatttc agctatgcca aatactaaag catacggttc tttgtcaatc
    14641 gcagtgcagt attatatgac tgccaaagta tcatttatcg ttccgagaac tgtttttgtt
    14701 cctgctccaa atgtagattc agctattttg aaaatggtct gtagagatca accagtagtg
    14761 agtgttcaag atgaggattt ctttttcaga gtgtcaaaag tagccttcgt acatcgtcgt
    14821 aaaacacttt ggaataatct aacgagtcat tttggaaaat cagaagatac taaagcaaaa
    14881 ttggaaaaag cattagaaat agcaaaaatt aagccatcta ttcgcggaga agccttatct
    14941 atccctgatt ttgcaagttt agcagatgcc ttgaaagagg ttgggatata aactatgttg
    15001 gttgacaaga agtggcgttt tgaagatagt gcaagttatt tcgcttgtcc taaatgtcaa
    15061 aatcccttga taaaagaaag taacagcctc aaatgctctg ataatcattg ttttgattta
    15121 tccaaatttg gatatgttaa tcttcttgga ggaaagaagg tagatgaaca ttatgataaa
    15181 aaatcatttg aaaatcggca gcttgtctta gaaaacgggt attataatca tattttagaa
    15241 gctatttcaa aggttttgga aaataattct cagtttcatt cggttttaga tattggttgt
    15301 ggagagggct tttattctcg tcaattagtt aacaagcatg agaagacttt cttggctttt
    15361 gatatttcta aagattcgat tcaattagct gctaaaagtg atcagtctag gcttgtgaaa
    15421 tggtttgttt ctgatttagc taaccttcct attcaagact catcaattga tatcatttta
    15481 gacatttttt ctcctgcaaa ttataaagaa tttaggcggg tgctgagtga tgatggtatc
    15541 cttgtaaagg ttgttcctgt agctgaacat gttcaagaat tgcgagaaaa agctagtcaa
    15601 tacttgaaac agaaagatta ctctaatcaa aaaattctag atcactttag agaaaatttt
    15661 gaaattattt ctgagcagaa agttgttcaa agctataact gttctcaaca agaacgtcaa
    15721 gcttttattg atatgacacc gttgttattc agcgtagata aaacgactat tgattgggcg
    15781 agtatatcag aaattacagt aggtgcctta atagtaattg gaaaaaagcg cagtgtatct
    15841 aaataagatc actgctttta tgttgggaat tcataaaatt gaggttattt gagagaaatc
    15901 gaacacctgc aaatagtttc tatttagctt atctctgaga gtagagctta tttctcattt
    15961 tatgatataa tagtggtatt ctaaacaatt aaggagaaca attgcaaggt agaattgtca
    16021 agtcgctagc aggtttttat tatgtggaat ctgacggtgt tgtttatcag acacgtgcgc
    16081 gtggaaattt tagaaaaaaa ggtcaaattc cctacgttgg ggattgggtt gaattttctt
    16141 cgcaagatca atcagaagga tatattctat caattgaaga gcgtaaaaat agcctcgtac
    16201 gaccacctat tgttaatatt gatcaagctg tcgttatcat gtctgctaaa gaacctgatt
    16261 ttaacgctaa cttattagac cggttcttag tacttctaga atataagatg attcagccta
    16321 tcatttatat ttccaagttg gatttgttag acgatttggt tgttattgat gatattcgcg
    16381 aactttatca gaatataggc tacgttttct gttattctca agaggaactc ttgcctctgt
    16441 tagcaaataa agtgactgtt tttatgggcc agacaggtgt cggaaaatca acgcttctca
    16501 acaaaattgc tcctgagttg aaacttgaaa ctggtgaaat ttcgggtagt ttaggccgtg
    16561 gccgacacac cacaagagca gttagtttct ataacgttca taaaggaaaa attgcagaca
    16621 ctcctggttt ttcttctttg gactatgaag tggataatgc agaggactta aacgaatcct
    16681 ttccagagct tcgtagactt agtcattttt gtaaattccg ctcgtgtacg catacacatg
    16741 agcctaaatg tgctgtaaaa gaagccttaa ctcaaggtca gttatggcaa gtccgctatg
    16801 ataactacct ccaattttta agtgaaattg agaaccgtcg tgaaacatat aagaaagtta
    16861 ttaaaagaaa gtaggtttct atgtcaacca ataaaattgc cccatcgata ttagcagcag
    16921 actatgctaa ttttgctaac gaattgaaac gtattgaaga gacaacagca gaatacgttc
    16981 atattgatat catggatggt caatttgtcc ctaatattag ttttggtgcg gatgttgtat
    17041 ccagcatgcg taaacacagt aaacttgttt ttgattgtca tctgatggtt gttgatccag
    17101 aacgttatat tgaggctttt gctcaagctg gtgctgatat catgaccatt catgttgaag
    17161 caacgaaaca tatacatggt gcgcttcaaa agataaaaga ggcaggaatg aaagcaggtg
    17221 tggttattaa cccaggaaca cccgtagaat ctcttattcc tatcttagat ttggttgacc
    17281 agattttaat tatgactgtt aatcctggct ttggtggaca agcctttatt ccagaaatga
    17341 tgtcaaaagt aaagacagtt gcagcatggc gcaaagagta cggtcatcat tatgatattg
    17401 aagttgatgg cggtatagat aatactacaa ttaaggcagc agcagaggct ggtgcaaatg
    17461 tctttgttgc aggttcttat ctctttaaag caagtgattt acctgcacaa gtagaaacct
    17521 taagggtggc gctcgatgac taaaattgct ttatttgctg gcggtgactt aacttatttt
    17581 gaatatgatt ttgactattt tgtcggaatt gaccgtggaa gcctattttt attaaaaaat
    17641 gggctttctt tggacatggc agtgggtgat tttgattcta taacagaaga tgaactgctt
    17701 tatatcaaac actattgtag caatatcgtt tctgctagtg cagaaaaaaa tgatacagat
    17761 acagaattag ctttaaaaac tattttcaaa gaatttccag aagctcaggt gaccgtattt
    17821 ggagcatttg gtggcaggat tgaccatatg atgtctaata tctttttacc aagtgacaga
    17881 gatctagaac catttatgtc acaaatcaga ctcaaagatg aacaaaacat agtgacttat
    17941 ctaccatccg ggaaaaatca agtctcaagg attgaaggga tgtcctatgt ctcgttcatg
    18001 cctgagagtg agtctacttt gcagatttct ggggctaaat atgaattgaa caaaagtaat
    18061 tattttaaga aaaaaatgta ttccagtaat gaatttatga cttcgccaat tgaagtagag
    18121 ctcaaagatg gttacttaat cattatttat agtaaggata gaggttaata tgcttgtcat
    18181 cattttgatc attgtactag ctagtctgac agtgacgata atttcttacc caaaaatgac
    18241 ggaattaaca aagtccgttg aaaaacaact tgaagataat gctgataatc tatcagacca
    18301 actgacatat cagatagaag tggcgcaaaa agatcaaatt ctaacgttga ctaatcagct
    18361 aaaccgtatg caacaggaaa tttatcagtt gcttaccgat atgcgtacag aattaaatca
    18421 gcatttaacg gagagtcgag atcgatcaga caaacgcttg gaattgatta actccaattt
    18481 atctcagtca gttcagaaaa tgcaagattc caatgaaaaa cgcttggatc aaatgcgcca
    18541 aactgttgag gaaaagctag aaaaaacgct acaaacgcgt ttgcaaactt cttttgaaac
    18601 tgtatcgcgt caactagaga gcgtcaatca aggtcttggg gagatgaaaa ctgttgccca
    18661 agatgttggt acactgaaca aagtcttgtc aaatactaag acaaggggaa tattagggga
    18721 gttacaactc ggccaaatta tagaagatat tatgacagtt agtcaatatg agagagaatt
    18781 tcctacggtg tctggctctt ctgagcgtgt tgaatatgct attaaattac ctggaaatgg
    18841 tcagggagat tatatctatt tgcctattga ctctaagttt cctctagaag attattaccg
    18901 attggaagat gcttatgaat tgggtgacaa ggttcaaata gaactctatc gtaaatcttt
    18961 actggcaagt attcgtaaat ttgcaaaaga tataaacaat aagtacttaa atcctcctga
    19021 aacgacaaat tttggtatca tgttcttacc aactgaaggg ctctattctg aagtggtaag
    19081 aaatgcaaca ttctttgata gtctaagacg tgacgaaaat attgtagtag ctggaccgtc
    19141 aaccttatct gctttactaa attcattatc tgttggcttt aaaaccttaa atattcaaaa
    19201 gaatgctaat gatattagta aaattttagg gaatgtcaag gttgaatttg gaaaatttgg
    19261 tggcatgtta tcaaaagctc aaaaacaatt aaatacagca agtaaaagta ttgatagttt
    19321 actcacaacg cggacaaacg ctattatcag agtcttaaat acagttgaag aacatcaaga
    19381 tcaagcaacc actagcttgt taaaccttcc tataacagaa gaagaggaaa ttaatgaaaa
    19441 ttaaccaaat gaaaaaagac gagctttttg aggggtttta ccttataaag aaagcagaag
    19501 ttcgtaaaac gcgtgcaggt aaagacttta ttgcctttac ctttcaagat gatactggcg
    19561 aaatttcagg aaatatgtgg gatgcacaga cctataatgt agaagagttt gtagccggta
    19621 aaattgttca tatgaaaggg cgtcgtgaag tttataatgg aactccccaa gtcaaccaaa
    19681 ttacattaag aaacatcaaa gatggtgaac ctaatgatcc tagagatttt aaagaaaaac
    19741 ctccaatcaa tgttgacaac gtgagagagt acatggaaca aatgctcttt aaaattgaaa
    19801 atgcaacctg gcagcgtgtg gtaagagcac tttatcgtaa atacaataag gaatttttta
    19861 cctatccagc cgccaaaaca aaccaccacg cttttgaatc aggattggca tatcacacgg
    19921 caacaatggt tcgtttggca gatagtatcg gagatatcta tccagaactt aataaaagtt
    19981 tgatgtttgc tggtattatg ctacatgatt tagccaaggt cattgagtta tcgggtcctg
    20041 ataatacaga atatactatt cgaggtaatc ttatcggtca tatttcactt attgatgagg
    20101 aattaacaaa aatactagca gagcttaata ttgatgatac aaaagaagag gttacagtgt
    20161 tacgccatgt tattctgagt catcatggac aattagaata tggaagcccg gttcgtccta
    20221 gaatcatgga ggcagaaatt atccatatga tcgataacat tgatgcaaat atgatgatga
    20281 tgacaacagc tttaaaccgt gtcaacgaag gtgaaatgac aaatcgcatt tttgccatgg
    20341 ataaccgttc tttttataaa ccgaacatta aataatttag gtagaataat taagacctaa
    20401 aataaaatgt tcgtttttag catttgttat gttatactat atgtaaagaa tgctatgaat
    20461 aaggagaaaa tatgaaatta agacgtagcg aacgcatggt tgttatttca aattacctaa
    20521 taaataatcc ctatacatta actagtttaa atacatttgc tagtaaatac ggtgccgcga
    20581 aatcttcgat ttcagaagat attgctatta tcaaaaaagc ttttgaacaa gctcaaatcg
    20641 gagatattaa aactgtgact ggtgcaagtg gaggtgttat ctttacacca actattgctg
    20701 aagcagaagc gaaagaaatc gttgaagaat taagacagcg tttatctgaa aacgatcgta
    20761 ttcttccggg aggttacatt tatctttcag atctactcag tacaccaaaa atgttacaaa
    20821 gtattggtcg cattatagcg aacgcttaca gaggtcaaaa aattgatgct gtcatgacgg
    20881 ttgcaacaaa aggtgttcct ttagcaaacg cagtagcaaa tgttttagat gttccttttg
    20941 ttattgtgag acgtgatttg aagattacgg aaggttctac tgtgtcagta aactacgcta
    21001 gtggttcaag tggccgtatt gaaaaaatgt ttctttctaa gcgtagctta aaacctaaca
    21061 gtcgagtttt aatagttgat gatttcttga aaggtggagg tactgtttcg gggatgatta
    21121 gtttactctc agaattcgat agcacattag taggtgtcgc tgtatttgca gaaaatgctc
    21181 aggaacaacg tgagaagatg gcctataaat ctcttctgag agtatctgaa attgatgtaa
    21241 aaaataatag agtctctgta gaagcgggga atatatttga ttaaagagtc aaggtgacat
    21301 ccttgattct ttttgcttgt aaaagtaata aaagcttgac acattatgtg taaaatgata
    21361 ctatgataaa cggtactttt acttttggtc tctcaaaagt gtacagagac gtgctgacaa
    21421 ttgttgcaaa agtacacgca gatataggct gtcaccaagt gctatatcaa ccaaaaataa
    21481 aaaacttaca ggagaaatgt agatgcctac aattaaccag ttggtacgta aaccacgtaa
    21541 atctaaagtt gaaaaatcag attcacctgc tttgaatatt ggttacaaca gtcacagaaa
    21601 agttcacact aaattatctg caccacaaaa acgtggtgtt gcaactcgtg ttggaactat
    21661 gacacctaaa aaacctaact ctgcccttcg taaatttgct cgtgtacgtt tgagcaacct
    21721 tattgaagtt actgcttaca ttccaggtat cggacacaac ttgcaagaac acagtgttgt
    21781 gcttatccgt ggtggacgtg taaaagacct tccaggggta cgttaccata tcgttcgtgg
    21841 tgcacttgat actgcaggtg tagctgatcg taaacaaggc cgttctaaat acggtgctaa
    21901 acgtcctaaa gggtaattag aagggggata agagaaaatg agtcgtaaaa atcaagcgcc
    21961 taaacgcgaa gtattgccag atccattgta taactcaaaa atcgttacac gtcttatcaa
    22021 ccgtgtaatg cttgatggta aacgtggtac tgctgcaact atcgtatatg atgcttttga
    22081 agcaattaaa gaagctactg gaacagatgc tcttgaagtg ttcgaaacag ctatggaaaa
    22141 catcatgcct gtacttgagg tacgtgcacg ccgtgttggt ggatcaaact accaagttcc
    22201 tgtcgaagtt cgtccagaac gtcgtacaac tttaggactt cgttggttgg ttaacgcatc
    22261 acgtgctcgt ggtgaacaca ctatgaaaga tcgtcttgca aaagaaatca tggatgctgc
    22321 aaacaacaca ggagcatcag ttaagaaacg tgaagatact cacaaaatgg ctgaagctaa
    22381 ccgtgccttt gcacacttcc gttggtaaga ttactctctt tttgagactt atcatagcta
    22441 ctaaacaaat tgtttggtag atagcaatgt tagcaattag aacgggtagg tcctgcctat
    22501 ccgtttttta taaatagtgt tataataaag aagtaaataa tttaaaatat aggagaaacc
    22561 aaatggctcg cgaattttca cttgaaaaaa ctcgtaatat cggtatcatg gctcacgtcg
    22621 atgctggtaa aacaacaaca actgagcgta ttctttacta tactggtaaa atccacaaaa
    22681 tcggtgaaac acacgaaggt gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    22741 gtatcacaat tacatcagct gcaacaacag cacaatggga tggtcaccgt gttaacatca
    22801 tcgatacacc aggacacgtg gacttcacaa ttgaagttca acgttcactt cgcgtacttg
    22861 atggtgcggt aacagttctt gatgctcaat caggtgtaga acctcaaact gaaacagttt
    22921 ggcgtcaagc aacagaatac ggagttccac gtatcgtttt tgctaataaa atggataaaa
    22981 tcggtgctga cttcctctat tcagtacaat cacttcatga ccgtcttcaa gcgaatgcac
    23041 acccaattca attgccaatt ggttcagaag acgatttccg tggtattatt gacttaatca
    23101 aaatgaaagc tgaaatctat actaatgacc ttggtacaga tatccttgaa gaagatattc
    23161 cagcagagta tgttgatcaa gcaaatgaat atcgtgaaaa attagtagaa gcagttgctg
    23221 atactgatga agatttgatg atgaaatacc ttgaaggtga agaaatcact aacgaagagt
    23281 tgatggctgc tattcgtaaa gcaactatca atgttgaatt ctaccctgta ttgtgtggtt
    23341 cagccttcaa gaataaaggt gttcaattga tgcttgatgc tgttattgat taccttccaa
    23401 gcccacttga tattcctgca atcaaaggta ttaaccctga tactgacgaa gaagaaacac
    23461 gtcctgcttc agatgaagag ccatttgcag cgcttgcctt caagatcatg acagatcctt
    23521 tcgtgggtcg cttaacattc ttccgtgtgt actcaggtgt tcttaattca ggttcatacg
    23581 tgttgaatac atctaaaggt aaacgtgaac gtatcggacg tatcttacaa atgcacgcaa
    23641 atagtcgtca agaaattgaa actgtttatg ctggggatat cgcagcggct gttggtttga
    23701 aagatacaac tacaggtgat tcattgactg atgaaaaatc aaaagttatt cttgaatcaa
    23761 ttgaagttcc agaaccagtt atccaattga tggttgagcc aaaatctaaa gctgaccaag
    23821 ataaaatggg tatcgccctt caaaaacttg ctgaagaaga tccaacgttc cgcgttgaaa
    23881 ctaacgttga aactggtgaa acagttatct caggaatggg tgaacttcac cttgatgtcc
    23941 ttgttgaccg tatgaaacgt gaattcaaag ttgaagcaaa cgttggtgca cctcaagtat
    24001 cataccgcga aacattccgc gcttctacac aagcacgtgg attcttcaaa cgccaatctg
    24061 gtggtaaagg tcaatttggt gatgtttgga ttgaatttac accaaacgaa gaaggtaaag
    24121 gattcgaatt cgaaaatgct atcgtcggtg gtgtggttcc tcgtgaattt attccagccg
    24181 ttgaaaaagg acttgtagaa tctatggcaa acggtgttct tgctggatat ccaatggttg
    24241 acgtaaaagc taaactttac gatggttcat accatgatgt tgactcatct gagacagcct
    24301 tcaagattgc tgcatcactt gcacttaaag aagctgctaa atcagcgcaa ccagctatcc
    24361 ttgagccaat gatgcttgtt acaattacag ctccagaaga caatcttggt gacgttatgg
    24421 gtcacgtgac tgcacgtcgc ggtcgtgttg atggtatgga agcacgtggt aatacacaag
    24481 ttgttcgtgc ctttgttcca cttgctgaaa tgtttggtta tgcaacagtt cttcgttctg
    24541 caacacaagg acgtggcaca ttcatgatgg tatttgacca ctacgaagat gttcctaagt
    24601 cagttcaaga agaaatcatc aagaaaaact ctggtgaata atttctgaaa acttatgaaa
    24661 ggtactcgaa agagtatctt tttttctttt tgaaagtggt aaattcattg caatttttct
    24721 cgaaaaaaga tataataatt gtgttgaaag atttttgcac atatgtacaa gttaatcttt
    24781 tcacattggt tagggggatc cctaaaataa aatatttgag attttcataa ggaggaaatc
    24841 actaatggta gttaaagttg gtattaacgg tttcggtcgt atcggtcgtc ttgcattccg
    24901 tcgcatccaa aacgtagaag gtgttgaagt tactcgtatc aacgacctta cagatccaaa
    24961 catgcttgca cacttgttga aatatgacac aactcaaggt cgtttcgacg gtactgttga
    25021 agttaaagaa ggtggattcg aagttaacgg tcaatttgtt aaagtttctg ctgaacgcga
    25081 accagcaaac attgactggg ctactgatgg cgtagaaatc gttcttgaag caactggttt
    25141 ctttgcatca aaagaaaaag ctgaacaaca catccatgaa aatggtgcta aaaaagttgt
    25201 tatcacagct cctggtggaa acgacgttaa aacagttgtt ttcaacacta accacgatat
    25261 ccttgatgga actgaaacag ttatctcagg tgcttcatgt actacaaact gtcttgctcc
    25321 aatggctaaa gctttacaag acaactttgg tgttaaacaa ggtttgatga ctactatcca
    25381 cgcatacact ggtgaccaaa tgatccttga cggaccacac cgtggtggtg accttcgtcg
    25441 tgctcgtgca ggtgctgcaa acatcgttcc taactcaact ggtgctgcaa aagctatcgg
    25501 acttgttatc ccagaattga acggtaaact tgatggtgct gcacaacgtg ttcctgttcc
    25561 aactggatca gtaactgaat tggttgcaac tcttgaaaaa gacgtaactg tcgaagaagt
    25621 aaatgcagct atgaaagcag cagctaacga ttcatacggt tatactgaag atccaatcgt
    25681 atcatctgat atcgttggta tttcatacgg ttcattgttt gatgctactc aaactaaagt
    25741 tcaaactgtt gacggtaacc aattggttaa agttgtttca tggtacgata acgaaatgtc
    25801 atacacttca caacttgttc gtacacttga atactttgca aaaattgcaa aataattttt
    25861 aattaatatg atgtaaaaga gggatttatc cctcttttat ttttataaat aaaaaataat
    25921 acgaatgttt tttcatttta ttatagaaaa ttatcagaaa ataaatgaaa aatgaattat
    25981 attgactata agttaaaata aggtttgatt ttgaaagtaa gcagtggtat gatttaattg
    26041 aaaaaatata gaggagaagt tttatgaaga ctacaaaagt tttttcagtt ttatcgttat
    26101 ctgttgctgt atttatgatg acagcatgtc agtcaaacca cagtactaaa aaagctgata
    26161 tgtctccaga taaggtggag caaaagacga aatttaaaaa tatttctcta agttctaata
    26221 aactgttggc taaggaaaac actatgtctg tcctttggta tcaaaattct gcagaagcca
    26281 aggctttata tttacaaggt tataatgttg ctaaaatgaa gttagatgat tggttacaaa
    26341 agcccagtga aaaaccatat tcaattatct tagatttaga tgaaacagtt ttagataata
    26401 gcccatatca agcaaagaat attaaagatg gctctagttt cacgccagag agttgggata
    26461 aatgggtgca aaagaaatca gctaaggctg ttgcgggtgc caaagaattt ttgaagtatg
    26521 ctaatgaaaa gggaataaaa atttattatg tctcagatcg tacagatgct caagttgatg
    26581 cgactaaaga aaatttagag aaggaaggta tacctgttca agggaaagac cacttgcttt
    26641 tccttaaaaa aggaatgaaa tctaaagaga gtcgccgtca ggcagttcaa aaagatacca
    26701 atttaattat gctttttgga gataatttag ttgattttgc tgatttttct aaatcatcta
    26761 gtacagatag agaacaacta ctaactaaac ttcaaagtga gtttggtagt aaatttattg
    26821 ttttcccaaa tcctatgtac ggttcttggg aaagtgctat ttatcaagga aaacatctgg
    26881 atgttcaaaa acaattgaaa gaacgacaaa aaatgttgca ttcgtatgat taataagtat
    26941 acgaattctc ttaaaaacaa ctaggaaaat ctatgaaatc tatttgtgaa aaagtaatca
    27001 ataattacaa ctgttcacaa aaatatgtta taataaataa tatcaaatta tataaggagt
    27061 tttactaatg gctaaattga ctgttaaaga cgttgatttg aaaggtaaaa aagtcctcgt
    27121 tcgtgttgac tttaatgtgc ctttgaaaga cggcgttatc actaacgaca accgtatcac
    27181 tgcggctctt ccaacaatca agtatatcat cgaacaaggt ggtcgtgcta tcctcttctc
    27241 tcaccttgga cgtgttaaag aagaagctga caaagaagga aaatcacttg caccggtagc
    27301 tgctgattta gctgctaaac ttggtcaaga tgttgtattc ccaggtgtta ctcgtggtgc
    27361 aaaattagaa gaagcaatca atgctttgga agatggacaa gttcttttgg ttgaaaacac
    27421 tcgttttgaa gatgttgacg gtaagaaaga atctaagaat gacgaagaac ttggtaaata
    27481 ctgggcttca cttggagatg gaatcttcgt taacgatgca tttggtacag cacaccgtgc
    27541 tcatgcatca aacgtaggta tttcagcaaa cgttgaaaaa gctgtagctg gtttccttct
    27601 tgaaaacgaa attgcttaca tccaagaagc agttgaaact ccagaacgcc cattcgtagc
    27661 tattcttggt ggctcaaaag tttctgataa gattggtgtt atcgaaaacc ttcttgaaaa
    27721 agctgataaa gttcttatcg gtggtggtat gacttacaca ttctacaaag ctcaaggtat
    27781 cgaaatcggt aactcacttg tagaagaaga caaattggat gttgctaaag acctccttga
    27841 aaaatcaaac ggtaaattga tcttgccagt tgactcaaaa gaagcaaacg catttgctgg
    27901 ttatactgaa gttcgcgaca ctgaaggtga agcagtttca gaagggttcc ttggtcttga
    27961 catcggtcct aaatcaatcg ctaaatttga tgaagcactt actggtgcta aaacagttgt
    28021 atggaacgga cctatgggtg tctttgaaaa ccctgacttc caagctggta caatcggtgt
    28081 aatggacgct atcgttaaac aaccaggcgt taaatcaatc atcggtggtg gtgattcagc
    28141 agcagctgct atcaaccttg gtcgtgctga caaattctca tggatctcta ctggtggtgg
    28201 agcaagcatg gaattgctcg aaggtaaagt attaccaggt ttggcagcat tgactgaaaa
    28261 ataatagtca ttataattta tattaaagga catgctgatg catgtctttt tttagttgat
    28321 aatgtagagt tattgaactt tccttgactt catttatcat
//
LOCUS       DATRUK010000020        26753 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.020, whole genome shotgun sequence.
ACCESSION   DATRUK010000020 DATRUK010000000
VERSION     DATRUK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26753
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.020"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..428)
                     /locus_tag="VBE34_000853"
     CDS             complement(<1..428)
                     /locus_tag="VBE34_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEO7263582.1"
                     /translation="MKQEKTGRNLNFWKWAFLLLLAINLSFTAVIASRLIQVREPNTG
                     KISTGVQDKVKVGTFTTNKSQLNKTIALYLKQYQTKKMNYKIYAASSSILFEGSYQLL
                     GYEVPLYIYFEPYRLTNGAVQLKVTSFSVGTLPLPEKDVL"
     gene            complement(403..1242)
                     /locus_tag="VBE34_000854"
     CDS             complement(403..1242)
                     /locus_tag="VBE34_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO7263583.1"
                     /translation="MNKKKILTGLSFFLVSLLLSFGIFSLIIPKSNPKLTKKDFLTKK
                     VIPLNYVALGDSLTEGVGDTTSQGGFVPLLSESLHNRYSYQVTSVNYGVSGNTSQQIL
                     KRMTTDPQIEKDLEKADLLTLTVGGNDVLAVIRKELSHLSLNSFEKPAEAYKERLKEI
                     LAKARQDNPKLPIYVLGIYNPFYLNFPQLTKMQTVIDNWNKATKEVVDASENVYFVPI
                     NDRLYKGINGKEGITESSNSQASITNDALFTGDHFHPNNIGYQIMSNAVMEKINETRK
                     NWP"
     gene            complement(1235..2071)
                     /locus_tag="VBE34_000855"
     CDS             complement(1235..2071)
                     /locus_tag="VBE34_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO7263584.1"
                     /translation="MSKIKIVTDSSITIEPELIKELDITVVPLSVMIDGTLYSDNDLK
                     AQGEFLNLMRGSKELPKTSQPPVGVFAEIYEKLMNEGAEHIIAIHLTHTLSGTIEASR
                     QGANIAGADVTVIDSTFTDQCQKFQVVEAAKLAKEGADLDTILARVEEVRQKSELFIG
                     VSTLENLVKGGRIGRVTGLLSSLLNIKVIMELTNHELVPIVKGRGLKTFSKWLDNFVE
                     SAQTRKIAEIGISYCGKADMANNFKEKLAVLGAPISVLETGSIIQTHTGEDAFAVMVR
                     YE"
     gene            complement(2184..3842)
                     /gene="recN"
                     /locus_tag="VBE34_000856"
     CDS             complement(2184..3842)
                     /gene="recN"
                     /locus_tag="VBE34_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEO7263585.1"
                     /translation="MLLEISIKNFAIIEEISLNFETGMTVLTGETGAGKSIIIDAMNM
                     MLGARASVEVIRHGANKAEIEGFFSIEKNQSLVQLLEENGIELADELIIRREIFQNGR
                     SVSRINGQMVNLSTLKAVGHYLVDIHGQHDQEELMKPNMHILMLDEFGNTEFNVIKER
                     YQSLFDAYRQLRKRVLDKQKNEQENKSRIEMLEFQIAEIESVALKSDEDQTLLKQRDK
                     LMNHKNIADTLTNAYLMLDNEEFSSLSNVRSAMNDLMALEEFDREYKDLSTNLSEAYY
                     VIEEVTKRLGDVIDDLDFDAGLLQEIENRLDVINTITRKYGGDVNDVLDYFDNITKEY
                     SLLTGSEESSDALEKELKILEHDLIESANQLSLERHKLAKQLENEIKQELTELYMEKA
                     DFQVQFTKGKFNKEGNEIVEFYISTNPGEGFKPLVKVASGGELSRLMLAIKSAFSRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIHKIGSHGQVLAISHLAQVIAIADYQYFIEKIS
                     SDSSTVSTVRLLSYEERVEEIAKMLAGNNVTDTARTQAKELLGS"
     gene            complement(3854..4327)
                     /locus_tag="VBE34_000857"
     CDS             complement(3854..4327)
                     /locus_tag="VBE34_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEO7263586.1"
                     /translation="MKKSERLNLIKQIVLNHAVETQHELLRRLEAYGVTLTQATISRD
                     MNEIGIIKVPSAKGRYIYGLSNENDPIFTTAVAKPIKTSILSISDKLLGLEQFININV
                     IPGNSQLIKTFIMSHCQEHIFSLTADDNSLLLIAKSEADADHIRQSMIAMLEKKD"
     gene            complement(4314..5141)
                     /locus_tag="VBE34_000858"
     CDS             complement(4314..5141)
                     /locus_tag="VBE34_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEO7263587.1"
                     /translation="MAKERVDVLAYKQGLFDTREQAKRGVMAGLVINVINGERYDKPG
                     EKVADDTELKLKGEKLKYVSRGGLKLEKALQVFEISVADKLTIDIGASTGGFTDVMLQ
                     SGARLVYAVDVGTNQLVWKLRQDHRVRSMEQYNFRYAQKEDFKEGLPEFASIDVSFIS
                     LNLILPALKEILVDGGQVVALIKPQFEAGREQIGKNGIVKDKLVHEKVLTTVTNFTKD
                     YGYTVKHLDFSPVQGGHGNIEFLMHLQKCQDPQNLVLDQIQDVIEKAHKEFKKNEEE"
     gene            complement(5134..6006)
                     /locus_tag="VBE34_000859"
     CDS             complement(5134..6006)
                     /locus_tag="VBE34_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO7263588.1"
                     /translation="MVTIEKIDEAIHRHYKQTHSVVSPDLIKAILYSVDGGGKRIRPR
                     ILLEILEGFGVELIDGHYDVAAALEMIHTGSLIHDDLPAMDNDDFRRGRLTNHKKFDE
                     ATAVLAGDSLFLDPFDLVVKAGFKADVTVRLIELLSMSAGSFGMVGGQMLDMKGENKV
                     LSIDDLSLIHINKTGRLLAYPFVAAGILAEKSEEVKGKLHQAGLLIGHAFQVRDDILD
                     VTASFEELGKTPNKDIVAEKTTYPNLLGLDKSQEILDDTLKKAQAIFQNLEKKANFNA
                     RKIIDIIEGLRLNG"
     gene            complement(6006..6221)
                     /locus_tag="VBE34_000860"
     CDS             complement(6006..6221)
                     /locus_tag="VBE34_000860"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEO7263589.1"
                     /translation="MSDKKTFEENLQELETIVSRLETGDVALEDAIAEFQKGMLISKE
                     LQKTLKEAEETLVKVMQADGTEVEMDT"
     gene            complement(6199..7539)
                     /locus_tag="VBE34_000861"
     CDS             complement(6199..7539)
                     /locus_tag="VBE34_000861"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEO7263590.1"
                     /translation="MSDYLSVSTLTKYLKLKFDKDPYLERVYLTGQVSNFRRRPNHQY
                     FSLKDDKSVIQATMWSGQFKKLGFELEEGMKVNVVGRVQLYEPSGSYSIIVEKAEPDG
                     IGALAIQFEQLKKKLSQAGYFDDRHKQLLPQFVRKIGVVTSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGEGAAQEIAQTIALANEKKDLDLLIVGRGGGSIEDLWAFNEECVV
                     EAIFESRLPVISSVGHETDTTLADFVADRRAATPTAAAELATTVTKIDILSWITEREN
                     RMYQSSLRLIRTKEERLQKSKQSVIFRQPERLYDGFLQKLDNLNQQLTYSMRDKLQTV
                     RQKQGLLHQKLQGIDLKQRLHTYQERVVQSRRLLSSTMTSQYDSKLARFEKAQDALIS
                     LDSSRIVARGYAIIEKNHTLVSTTNGINEGDHLQVKMQDGLLEVEVKDVRQENI"
     gene            complement(7665..8501)
                     /locus_tag="VBE34_000862"
     CDS             complement(7665..8501)
                     /locus_tag="VBE34_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEO7263591.1"
                     /translation="MIVGEQEARALIKPRPKSSHKGDYGSVLLIGGFYPYGGAIIMAA
                     LACVKTGAGLVTVATQSCNIPSLHSQLPEVMAFDSDDYKWLEKSIVQSDVIVIGPGLG
                     VSESSRKILNRTMEKIQSHQSVIIDGSALTLLSEGAFPQTKAKNLVLTPHQKEWERLS
                     GIAVSQQTKENTQTALKSFPKGTILVAKSSHTRIFQDLDEKEIIVGGPYQATGGMGDT
                     LCGMIAGMLAQFKEASPLDKVSVGVYLHSAIAQELSKEAYVVLPTTISDEIPKEMARL
                     SK"
     gene            complement(8498..9352)
                     /locus_tag="VBE34_000863"
     CDS             complement(8498..9352)
                     /locus_tag="VBE34_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEO7263592.1"
                     /translation="MTELIDGKALSQKMQAELGRKVERLKEQHGIIPGLAVILVGDNP
                     ASQVYVRNKERSALEAGFKSETLRLSESISQEELIDIIHQYNEDKSIHGILVQLPLPQ
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPMMVPCTPAGIMEMFREYHVDLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRNLSEVTKEADILIVAIGQGHFVTKD
                     FVKEGAVVIDVGMNRDENGKLIGDVVFEQVAEVASMITPVPGGVGPMTITMLLEQTYQ
                     AALRSVSL"
     gene            complement(9491..11185)
                     /locus_tag="VBE34_000864"
     CDS             complement(9491..11185)
                     /locus_tag="VBE34_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO7263593.1"
                     /translation="MNYKEIYQEWLENDSLGKDIKSDLEAIKGDESEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAIARGIAVSYDVRYQSKEFA
                     ELTCSIMAANGIKSYIYKGIRPTPMCSYAIRALGCVSGVMVTASHNPQAYNGYKAYWK
                     EGSQILDDIADQIANHMDAITDYQQIKQIPFEEALASGLASYIDESIEEAYKKEVLGL
                     TINDTNIDKSVRVVYTPLNGVGNLPVREVLRRRGFENIYVVPEQEMPDPDFTTVGYPN
                     PEVPKAFAYSESLGKSVDADVLLATDPDCDRVALEVKDSKGEYIFLNGNKIGALLSYY
                     IFSQRCALGNLPHHPVLVKSIVTGDLSKVIADKYNIETVETLTGFKNICGKANEYDIS
                     KDKTYLFGYEESIGFCYGTFVRDKDAVSASMMVVEMTAYYKERGQTLLDVLQTIYDEF
                     GYYNERQFSLELEGAEGQERISRIMEDFRQNPILQVGEMTLENSIDFKDGYKDFPKQN
                     CLKYYFNEGSWYALRPSGTEPKIKCYLYTIGCTEADSLSKLNAIESACRAKMNSTK"
     gene            complement(11472..12206)
                     /locus_tag="VBE34_000865"
     CDS             complement(11472..12206)
                     /locus_tag="VBE34_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7263594.1"
                     /translation="MVEPIISIQGLHKSFGKNEVLKGIDLDIHQGEVVVIIGPSGSGK
                     STFLRTMNLLEVPTKGTVTFEGIDITDKKNDIFKMREKMGMVFQQFNLFPNMTVLENI
                     TLSPIKTKGLSKLDAQTKAYELLEKVGLKEKANAYPASLSGGQQQRIAIARGLAMNPD
                     VLLFDEPTSALDPEMVGEVLTVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDAGII
                     VEQGTPKKVFEQTKEIRTRDFLSKVL"
     gene            complement(12199..12891)
                     /locus_tag="VBE34_000866"
     CDS             complement(12199..12891)
                     /locus_tag="VBE34_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7263595.1"
                     /translation="MNFSFLPQYWSYFNYGVMVTIMISTCVVFFGTIIGVLIALVKRT
                     NLHFLTILANFYVWVFRGTPMVVQIMIAFAWMHFNNLPTISFGVLDLDFTRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPSGQIEAAYSLGIRPKNTLRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPVAPLLFAAFYYLMLTTILSALLKQM
                     EKYLGKGVKIDG"
     gene            complement(13032..13262)
                     /locus_tag="VBE34_000867"
     CDS             complement(13032..13262)
                     /locus_tag="VBE34_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HEO7263596.1"
                     /translation="MESHLVRIINRLELMATDGGNLKRNFEREGVVVAEVSFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            13560..15821
                     /locus_tag="VBE34_000868"
     CDS             13560..15821
                     /locus_tag="VBE34_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO7263597.1"
                     /translation="MLCQNCKLNESTIHLYTNVNGKQKQVDLCQNCYQIIKTDPNNPL
                     FSGLNHVSHAPGGINPFFDDFFGDLNNFRAFNGQDLPNTPPTQAGGNRGGGNGNGRNN
                     NRNQTATPPQAKGILEEFGINVTEIARHGDIDPVIGRDSEIIRVIEILNRRTKNNPVL
                     IGEPGVGKTAVVEGLAQKIVDGNVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKL
                     MEEIRQRQDVILFIDEIHEIVGAGTAGEGSMDAGNILKPALARGELQLVGATTLNEYR
                     IIEKDAALERRMQPVKVDEPSVEETITILKGIQKKYEDYHHVKYNNDAIEAAAVLSNR
                     YIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDQRLIEAENLKAQATREEDYERAA
                     YFRDQIAKYKEMQQQKVDDQDTPIITEKTIEHIIEEKTNIPVGDLKEKEQSQLINLAD
                     DLKQHVIGQDDAVIKIAKAIRRNRVGLGSPNRPIGSFLFVGPTGVGKTELSKQLAIEL
                     FGSADSMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLILLDEIE
                     KAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGSGKTEASVGFGASREGR
                     TNSVLGQLGNFFSPEFMNRFDGIIEFKALDKENLLNIVDIMLSDVNARLAINGIHLDV
                     TDKVKEKLVDLGYDPKMGARPLRRTIQEHIEDAITDYYLENPSEKELRAIMTSNGNII
                     IKSSKKTEESTKG"
     gene            16007..16462
                     /locus_tag="VBE34_000869"
     CDS             16007..16462
                     /locus_tag="VBE34_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7263598.1"
                     /translation="MTNPTFGEKIDNVDYRSRFGVYAIIPNPTHDKIILVQAPNGAWF
                     LPGGEIEENENHLEALTRELIEELGYSATIGHYYGQADEYFYSRHRDTYYYNPAYIYE
                     VTAYHKEQAPLEDFNHLAWFPIQEAKEKLKRGSHRWGVQAWEKNHHSRK"
     gene            16526..16828
                     /locus_tag="VBE34_000870"
     CDS             16526..16828
                     /locus_tag="VBE34_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEO7263599.1"
                     /translation="MRLINTTSSHPELVRNQLQNTDAKLVEVYSAGNTDVVFTTAPKH
                     YELLISNKYRAIKDEELEAIREFFLKRKIDQSIIIQEQMKSLHTAKLIEISYPTTA"
     gene            complement(16898..19690)
                     /locus_tag="VBE34_000871"
     CDS             complement(16898..19690)
                     /locus_tag="VBE34_000871"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEO7263600.1"
                     /translation="MKLKETLNLGQTAFPMRAGLPNKEPQWQEAWDQADIYKKRQALN
                     EGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFRAPYVPGWDTHGLPIE
                     QVLAKKGVKRKEMDLAEYLEMCRDYALSQVDKQRDDFKRLGVSADWENPYITLTPDYE
                     ADQVRVFGAMADKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTVTASRGLTVGPDMEYVVVAPVGSERKYLLAEVLVDSLAAK
                     FGWENFEIVTHHTGKELNHIVTEHPWDTEVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLDVVVTVDSRGLMMENAGPDFEGQFYDKVTPLVKEKLGDLLLASEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKTNFQPEWGKKRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFAEYGSIVWWQRDAKDLLPAGY
                     THPGSPNGLFEKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAVLSQGFVLDGKGEKMSKSLGNTILPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILKQTSETYRKIRNTLRFLIANTSDFNPKQDAVAYENLGAVDRYMT
                     IKFNQVVDTINKAYAAYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSPERRR
                     MQTVFYDILVKLTKLLTPILPHTAEEIWSYLEHEEEEFVQLAEMPVAQTFSGQEEILE
                     EWSAFMTLRTQAQKALEEARNAKVIGKSLEAHLTIYASQEVKTLLTALNSDIALLMIV
                     SQLTIADEADKPADSVSFEGVAFTVEHAEGEVCERSRRIDPTTRMRSYGVAVCDASAA
                     IIEQYYPEAVAQGFEA"
     gene            complement(19975..20745)
                     /locus_tag="VBE34_000872"
     CDS             complement(19975..20745)
                     /locus_tag="VBE34_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEO7263601.1"
                     /translation="MPLTALEIKDKTFSSKFRGYSEEEVNEFLEIVVDDYEDLIRRNR
                     EQEQYIKDLEEKIAYFNEMKESLSQSVILAQETAERVKISAQDEASNLMGKATFDAQH
                     LIDEAKLKANQILRDATDDAKRVAIETEDLKRQSRVFHQRLLSELEGQLKLANSSAWE
                     ELLKPTAIYLQNSDASFKEVVEKVLDEDDALPVVDDTESFDATRQFSPDEMEELQRRV
                     EESNKQLEESGLLDTNNFQMEEPINLGETQTFKLNIED"
     gene            complement(20755..21543)
                     /locus_tag="VBE34_000873"
     CDS             complement(20755..21543)
                     /locus_tag="VBE34_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEO7263602.1"
                     /translation="MTLDDIYQHFRPEEYAFINKIDHLAQYVENTYSFITTEFLNPRE
                     FKILESVLERRGSHYYTSGQYFQTEYVKVIIAPEYYQLDMVDFNLSLIEIKYNAKFNH
                     LTHAKIMGTLLNYLGVKRSILGDILVEEGCAQVLVDSQMTNHLVHSVTKIGTASVQLA
                     EVPLSKLLTPKQDIQKLTVIASSLRLDKILATILKISRTQSTKLIEADKVKVNYAIVN
                     RVSEQLVEGDLISVRGYGRFTLNHNLGLTKNQKYKLEVDKMIHN"
     gene            complement(21545..21799)
                     /locus_tag="VBE34_000874"
     CDS             complement(21545..21799)
                     /locus_tag="VBE34_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEO7263603.1"
                     /translation="MILVLLILLKTIRVYSYILLAYALLSWLPGAYDSWIGRLVVQIV
                     DPIIRPLRRFNLQFLGLDFTVIVAFFLLNMLSRFLILLFT"
     gene            complement(21802..22407)
                     /locus_tag="VBE34_000875"
     CDS             complement(21802..22407)
                     /locus_tag="VBE34_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEO7263604.1"
                     /translation="MALKDRFDKIISYFDTDDVSENEVHEVQERTSVQRDSRAATAQE
                     ASQRSHMTNSAEEEMIGSRPRTSTYNPNRQERQRVQRDNAYQQATPRIQNKDSVRQQR
                     EQVTIALKYPRKYEDAQEIVDLLIVNECVLIDFQYMLDAQARRCLDYIDGASRVLYGS
                     LQKVGSSMFLLTPANVMVDIEEMNIPKTGQETSFDFDMKRR"
     gene            complement(22419..23093)
                     /locus_tag="VBE34_000876"
     CDS             complement(22419..23093)
                     /locus_tag="VBE34_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEO7263605.1"
                     /translation="MMNLQENKTAIFDNVSKLALKAGRAPESVHIVAVTKYVNCQTTE
                     ALIRTGVNHIGENRVDKFLEKYQALKDEKLTWHLIGSLQRRKVKDVINYVDYFHALDS
                     VKLAAEIQKRAQKPVKCFLQVNISREDSKHGFTIEQIDDALNLISRYDKIELIGIMTM
                     APLKATKEEISSIFEETESLRKRLQARNIERMPFTELSMGMSRDYDIAIQNGSTFVRI
                     GTSFFK"
     gene            complement(23099..24379)
                     /gene="ftsZ"
                     /locus_tag="VBE34_000877"
     CDS             complement(23099..24379)
                     /gene="ftsZ"
                     /locus_tag="VBE34_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEO7263606.1"
                     /translation="MVFSFDTASVQGAVIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEVLTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVITRPFGFEGNKRSNFAIEGIQELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERITEAARKAIYSPLLETTIDGAEDVIVNVTGGMDMTLTEA
                     EEASEIVSQAAGKGVNIWLGTSIDMDMKDEIRVTVVATGVRKDKTNQVSGFTTSAPTN
                     QAPSERQSTSNSNFDRRGNFDMTESREMPTQQNQPHAQNQQQSSAFGNWDLRRDNISR
                     PTEGELDSKLSMSTFSENDDMDDELETPPFFKNR"
     gene            complement(24401..25774)
                     /gene="ftsA"
                     /locus_tag="VBE34_000878"
     CDS             complement(24401..25774)
                     /gene="ftsA"
                     /locus_tag="VBE34_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEO7263607.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFIANEMNVIGVSNVPSSGVKD
                     GIIIDIEAAATAIKEAVKQAEEKAGITIDKINVGLPANLLQIEPTQGMIPVPNESKEI
                     KDEDVESVVKSALTKSITPEREVISLIPLEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPTTILHNLRKTVERAGIKVEHVVIAPLALAKSVLNEGEREFGATVIDMGGGQTTVAS
                     MRNQELQYTNIYSEGSDYVTKDISKVLRTTVEIAEALKFNFGQANVEEASTSDTVQVN
                     VVGNEEPVEITESYLSQIISGRIRQILEHVKQDLGRGRLLDLPGGIILVGGGAIMPGV
                     VEVAQQIFGTRVKLHVPNQVGIRNPMFANVISIVDYVGMMSEVDIIAQHAVTGDEMLR
                     HKPVDFDYKEKTNTMSTMPYSEPLTSSMEDSNLEPIRARENAQEPTEPKTNIGERIRG
                     IFGSMFD"
     gene            complement(26047..>26753)
                     /locus_tag="VBE34_000879"
     CDS             complement(26047..>26753)
                     /locus_tag="VBE34_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEO7263608.1"
                     /translation="NIQKNDYFFSLIFKHKAIEQRLAAEDVWVKTAQMTYQFPNKFHI
                     QVQENKIIAYAHTKQGYQPVLETGKKADPVNSSELPKHFLTINLDKEDSIKLLIKDLK
                     ALDPDLISEIQVISLADSKTTPDLLLLDMHDGNSIRIPLSKFKERLPFYKQIKKNLKE
                     PSIVDMEVGVYTTTSTIESTPVKAEDTKNKSTDKTQTQNGQVAENSQGQTNNSNTNQQ
                     GQQIATEQAPNPQNVN"
BASE COUNT         8343 a         5384 c         4216 g         8810 t
ORIGIN      
        1 tgtaacacat ctttttctgg aagcggaaga gttcctactg aaaatgaggt cactttaagt
       61 tgaacagcgc catttgttaa acgataaggt tcaaaataga tgtataaagg cacttcataa
      121 cctaataatt gataagagcc ttcaaatagt atagaagatg aagcagcata aatcttataa
      181 ttcatcttct tagtttgata ttgttttaaa taaagtgcaa ttgtcttatt cagttgcgac
      241 ttattggtcg taaaagtacc tacttttact ttatcttgta ccccagtcga aatttttcct
      301 gtattaggct cacgtacttg aattaaacga cttgcaatca ctgctgtgaa ggaaaggtta
      361 attgccaaca ataagagaaa tgcccatttc caaaaattta aattacggcc agttttttct
      421 tgtttcattt attttctcca taacggcgtt agacatgatt tgatagccaa tattattggg
      481 atgaaaatgg tctccagtaa agagagcatc attagtgata cttgcctgac tatttgatga
      541 ctctgtaata ccctctttac catttattcc cttataaagg cggtcattaa ttgggacaaa
      601 ataaacattt tctgaagcat caactacttc ttttgtagct ttattccaat tatcaataac
      661 ggtttgcatt ttagttaatt gtggaaagtt taggtaaaaa ggattataaa tgcctaaaac
      721 ataaataggc aatttaggat tatcttgtct tgcttttgca agaatttctt tcaaacgttc
      781 cttatatgct tctgctggtt tctcaaagga atttagtgat aaatgactga gctctttacg
      841 aataacagcc aagacatcat taccaccaac agttagcgtc aataaatcag ctttctctaa
      901 atctttttcg atttgaggat ctgtcgtcat acgttttaaa atttgttgac tagtattccc
      961 agacacacca taattaacag aagtcacttg gtaagagtat cgattatgga gtgattctga
     1021 tagcagtggg acaaaaccac cttgagaggt tgtatccccc acaccttcgg tcagagaatc
     1081 tccaagagca acatagttaa gtgggataac tttctttgtt aggaagtctt tttttgttaa
     1141 tttaggattt gattttggaa tgatcaagga aaaaatccca aaagataaaa gaagacttac
     1201 cagaaaaaaa gaaagtccgg taagtatttt tttcttattc ataacgaacc ataacagcaa
     1261 atgcatcctc accagtatga gtttggatga ttgagccagt ttctaatacg gatataggag
     1321 cacctagaac agctagtttt tctttaaagt tattagccat atcagcttta ccacagtagg
     1381 agatgccaat ttcagcaatt tttcgtgttt gggcactttc aacaaaatta tctaaccatt
     1441 tgctgaaggt ctttagaccg cgacctttaa caatcggtac taattcatgg tttgttagct
     1501 ccataattac ttttatgtta agcaatgaac ttaggagacc tgttacacgt ccaatacgac
     1561 caccttttac taaattttca agggtcgata cgccaataaa taattctgac ttctggcgta
     1621 cttcttccac acgagccaaa attgtatcta aatcagctcc ctctttagct aatttcgcgg
     1681 cttctacaac ctggaatttt tgacactggt ctgtaaaagt agaatcaata actgtaacat
     1741 ctgcaccagc aatattagct ccctggcgtg atgcttcaat agttcctgat agtgtatgcg
     1801 ttaaatgaat tgcaatgata tgttccgcac cttcattcat taatttttca taaatttctg
     1861 caaaaacacc tacaggtggt tgacttgttt ttggcaattc cttacttcca cgcattaggt
     1921 tcaagaattc cccttgtgct tttaagtcat tgtctgaata caaggtacca tctatcataa
     1981 ctgacaaggg aacaactgta atgtcaagct ctttaataag ttctggttca atggtaatgg
     2041 aagaatccgt tacaattttg attttactca tatcctatcc tattctatac tttaatattt
     2101 caataccctt attataacaa aatttaagtg tatcaacatt aatttctcct aacaatgtta
     2161 gctttttaaa gacgttacca aagctaacta ccaagtaatt ctttagcttg agtacgtgca
     2221 gtatcagtta cattgtttcc tgccaacatt ttagcgattt cttctacacg ttcttcatag
     2281 cttaaaagcc ttaccgttga aactgttgaa gagtcactgg aaattttttc aataaaatat
     2341 tggtaatccg ctattgcaat aacctgagct aaatgagaaa ttgctaaaac ttgtccatga
     2401 ctaccaatct tgtgaatttt ttgagcaata gcctgggcca cgcgaccaga aactcctgta
     2461 tccacctcat caaatacaat ggaagtttta tcttccttac gtgaaaaagc agatttaata
     2521 gctaacatca atcgagataa ctctcctcct gaagccactt ttaccaatgg cttaaagccc
     2581 tctccgggat tcgttgaaat ataaaattca acgatttcgt tcccttcttt attaaatttt
     2641 ccttttgtaa attgaacttg gaaatcagct ttctccatat aaagctctgt taattcttgt
     2701 ttgatttcat tttccaattg tttagctagc ttatgacgct ctaaacttaa ttgatttgct
     2761 gattcaatca aatcgtgttc taaaatcttt aattcttttt ctaaagcatc tgaggactct
     2821 tcactacctg tcaatagact atattcttta gtaatgttat caaaatagtc taaaacatca
     2881 ttcacatcac cgccatattt tcgcgttatt gtgttaataa catctagtct attttcaatt
     2941 tcttgtagta aaccagcgtc aaaatctaaa tcatcgataa cgtcacctaa acgtttagta
     3001 acttcttcaa taacgtagta agcttctgaa agattggtgg aaagatcttt atattctcga
     3061 tcaaattctt ctaaagccat aaggtcattc attgcagaac gaacattcga taaacttgaa
     3121 aactcttcgt tatctaacat aagatatgca tttgtcaaag tatctgcaat attcttatga
     3181 ttcattaatt tatcacgttg cttgagtagc gtttggtctt catctgattt aagggctaca
     3241 gactcaattt ctgctatttg aaattctagc atttcaatac gtgatttatt ctcttgttca
     3301 tttttttgct tatccaatac acgtttacga agctgacgat aagcatcaaa aagactttga
     3361 taacgctctt ttatgacatt aaattctgta ttaccaaatt catctaacat cagaatgtgc
     3421 atgttaggtt tcattagttc ctcttggtca tgctgaccat gaatgtctac caaataatgc
     3481 cccacagctt ttagtgttga caaattcacc atttgcccat taattcgact aacgctgcgc
     3541 ccattttgaa agatttctcg gcgaataatt aattcatctg ctaattcaat gccattttct
     3601 tccaataatt ggactaatga ttgatttttt tcaatagaga aaaatccttc aatttctgct
     3661 ttgttagcac catggcgaat cacttcaaca ctagcacggg ctcctaacat catattcata
     3721 gcatcaataa tgatagattt ccctgcacca gtctcaccag ttaaaacagt cattcctgtt
     3781 tcaaaattaa gtgaaatttc ttcgataata gcgaaattct taatagaaat ttctaaaagc
     3841 ataaggtcct ctactaatct tttttctcca acattgcaat cattgattga cgaatgtgat
     3901 cagcatctgc ttctgatttt gcaatcaaaa ggagactatt atcgtcagct gtcaaactaa
     3961 aaatatgttc ttgacaatgt gacattatga aggttttaat taattgactg ttacctggta
     4021 tgacattaat attgataaat tgctctaaac ctagtagctt atctgatatt gataaaatac
     4081 ttgttttaat aggctttgcc acagcagttg taaagatagg gtcgttttca tttgacaaac
     4141 cgtaaatata gcgacctttt gctgatggca cttttataat gccaatttca ttcatatcac
     4201 gtgaaatagt tgcttgagtt agagttaccc cataagcttc caatctgcgt aacaactcat
     4261 gttgtgtttc aacagcatgg ttaagaacaa tttgcttaat taaatttaaa cgttcactct
     4321 tcttcatttt tcttaaattc cttatgtgct ttttctataa catcttgtat ttggtcaagc
     4381 acaagatttt gtggatcttg acacttttgc aaatgcatta aaaactcaat atttccatgt
     4441 ccaccttgaa cgggcgaaaa atcaagatgt ttaaccgtat atccataatc tttcgtgaaa
     4501 ttggtcactg ttgtcaaaac cttttcatga accaacttgt ctttgacaat accattttta
     4561 ccaatttgct cacgacctgc ttcaaattgt ggtttaatta atgccactac ttgtccacca
     4621 tccactaaaa tttcttttag agctggtaaa atcaaattaa gagagataaa tgagacatct
     4681 atcgatgcaa attcaggcag tccctccttg aaatcttctt tttgggcata cctaaaatta
     4741 tattgttcca tagaacgaac acgatgatcc tgacgtaact tccaaactaa ttgatttgtt
     4801 cctacatcta ctgcgtaaac taaacgcgct cctgattgta gcataacatc agtaaaacca
     4861 cccgtagagg cgccaatatc tatagttagc ttatctgcaa ctgaaatttc aaaaacttgt
     4921 aaagcttttt ctaatttcaa tccacctcta ctaacatatt ttagtttttc accttttagt
     4981 tttaattcag tatcgtctgc aaccttttcg cctggtttat cataacgttc tccattgata
     5041 acgttaatca ccagtcctgc cataacacca cgtttcgctt gctctcgtgt atcaaaaagt
     5101 ccctgtttat aggcaagaac atctaccctc tctttagcca ttcaaccgta atccctctat
     5161 tatatctatt atttttctag cattaaagtt agcttttttc tctagatttt gaaaaattgc
     5221 ctgagctttt ttcaaagtat cgtcaagtat ttcctgtgac ttatccaaac ccaataaatt
     5281 tggataagtt gtcttttctg ctacaatgtc tttatttggt gtcttaccca attcttcaaa
     5341 actagcagtc acatctaaaa tatcatcacg tacttgaaaa gcatgaccga ttaaaaggcc
     5401 agcttgatgc agttttcctt ttacttcttc cgacttctca gctaaaatac ctgctgcaac
     5461 aaagggataa gctaacaggc gtcccgtttt attaatatga atcaagctta aatcgtcaat
     5521 agataaaact ttgttttctc ccttcatatc taacatttgt ccaccaacca tgccaaaact
     5581 acccgcagac atagacaata actctattaa cctaacagta acatcagctt taaaaccagc
     5641 ttttacaact aaatcaaatg ggtctaaaaa aagggaatct ccagctaaaa ccgctgtagc
     5701 ttcatcaaac tttttatggt ttgttaagcg gccacgacga aaatcatcat tatccatcgc
     5761 cggtaaatca tcatgaatta aagagccagt gtgaatcatc tccaaagcag cagccacatc
     5821 ataatgacca tctattagtt caactccaaa tccctctaaa atttctaaaa ggatgcgtgg
     5881 acgaatacgt ttcccacctc catcaactga gtagaggata gccttaatta aatcaggaga
     5941 aacgacacta tgtgtctgtt tgtaatgacg atgaatagct tcatcaattt tttcaatagt
     6001 aaccatcaag tatccatttc tacttccgtt ccgtcagctt gcatcacttt tacaagcgtc
     6061 tcttcagctt cttttaaagt tttttgtaac tcttttgaaa taagcattcc tttttgaaat
     6121 tcagcaatgg catcttctaa ggcaacatca cctgtttcta aacgtgagac aattgtctct
     6181 aattcctgca aattttcttc aaatgttttc ttgtctgaca tccttcacct caacttctaa
     6241 aagaccatct tgcatcttaa cttgcaaatg atctccttca tttattccat tagtagtaga
     6301 tactaaagta tggttttttt cgataatagc gtaaccacgc gctacaattc tagaactatc
     6361 caatgatata agcgcatctt gagccttttc aaaacgtgct agcttgctgt catactgact
     6421 agtcattgta cttgataata gtcgtctact ttgcacaaca cgctcttggt aagtatgaag
     6481 ccgctgtttt aaatctatgc cttgcagttt ttgatgaagc aaaccttgct tttgtcttac
     6541 tgtttgtagt ttatcacgca tagaatatgt taactgctga tttaggttat ctaatttttg
     6601 aaggaaacca tcatatagac gctctggttg cctaaaaata actgattgct ttgatttttg
     6661 caacctctcc tcttttgtcc tgataagacg aaggctagac tgatacattc tattttcacg
     6721 ttcagtaatc caagacaaga tatcaatttt agtaaccgtt gtagcaagct cagctgctgc
     6781 agttggagtt gctgctctgc gatcggctac aaaatctgct aaagttgtat cagtttcatg
     6841 tccgacacta gaaataacgg gaagacgaga ttcaaaaata gcttctacaa cacattcttc
     6901 attaaatgcc cataagtcct caatagaacc tcccccacgg ccaacaatta atagatccaa
     6961 atcctttttc tcgttagcta aagcaatagt ttgtgctatc tcctgagctg cgccctctcc
     7021 ttgaactttt gtggggaaaa gtaatatttc cactcctggg aatcgacgag atactgttgt
     7081 aataatatcc cgaataacag ctccacttgg actagtcaca actcctattt ttctaacaaa
     7141 ttgaggaagt aattgcttat gacgatcatc aaagtaacct gcttgagaga gtttcttctt
     7201 tagttgttca aattggatag caagagctcc tataccatcc ggttctgctt tttcaacaat
     7261 aatagagtag gagccgcttg gttcataaag ttgaacacgg ccaactacat taaccttcat
     7321 cccttcctct aattcaaaac ccagtttttt gaattgacct gaccacattg tcgcctgaat
     7381 gactgattta tcatctttta aagagaagta ttgatggttt gggcgacgac gaaaattaga
     7441 cacttgccca gttaaataaa ccctctctaa ataagggtct ttgtcaaatt ttaattttaa
     7501 atacttagtc agtgtcgaca cggataaata gtctgacata gcacctcctc atgagagttt
     7561 tattaacttc attataccaa aaaaacttaa aaaataccta tttgctctac aaatagctcg
     7621 ataaagcaaa agaagtgtca tgctaacacc tctctactta ttttttattt agatagtcta
     7681 gccatttctt ttggaatctc atcactaatc gttgtcggta gaacaacgta ggcttcttta
     7741 gataattctt gagcaattgc cgaatgtaga taaactccca ctgatacctt atctagagga
     7801 gaggcttctt taaattgcgc taacatacct gcaatcatac cacacaaagt atcccccatc
     7861 cctccagtcg cctggtaagg acctcctact ataatttctt tttcgtctaa atcttgaaaa
     7921 atacgcgtat gcgaactctt agcgactaag atcgtccctt tgggaaaaga tttaagagcg
     7981 gtttgggtat tttcttttgt ctgttgcgat acagcaatac ctgacaatcg ctcccattct
     8041 ttttgatgag gtgtcaacac taaattctta gcctttgttt gcggaaacgc accttctgat
     8101 aacagagtca aggctgatcc gtcaatgatg acactttgat gtgattgaat cttctccatg
     8161 gtccggttca aaatttttcg agatgattct gatactccta atccaggacc aattacaata
     8221 acatcacttt gaacaattga tttttccaac catttgtaat catcactatc aaacgccatt
     8281 acctctggta gttgactatg caaagagggg atattgcaac tttgggttgc tacagtaact
     8341 aatcctgcac cagttttgac acaggccaaa gctgccatta taatagcacc tccataggga
     8401 taaaaacctc ctatcagaag aacactacca taatcacctt tatggcttga tttagggcgt
     8461 ggcttaatta acgccctcgc ttcttgttca ccaacaatca tagactcaca cttctaagag
     8521 ctgcttgata agtttgttcc aaaagcattg taatcgtcat aggtcctacg cccccaggaa
     8581 caggtgttat catactagca acttctgcca cttgttcaaa tacaacgtct ccaatcaatt
     8641 taccattttc atcgcgattc ataccaacat caatcacaac agcaccttct ttaacgaagt
     8701 cttttgtaac aaagtgcccc tgaccaatcg caacaataag gatatcagcc tcctttgtta
     8761 cctcagaaag gtttcgagtt cttgaatgtg tcaaagtaac tgttgcgttt ttatcaagga
     8821 gaagttgtgc cattggctta ccaacaatat tggaccttcc gatgataaca gcatgttttc
     8881 cctctaaatc cacatggtac tctctaaaca tctccataat accagctggc gtacaaggta
     8941 ccatcatcgg tcgtcctgac caaagatgac ccgtattcat tgggtggaaa ccatcaacat
     9001 ctttcttagg atctattgct aaaataattt ttttatcgtt aatatgttgt ggtaacggaa
     9061 gttgaactaa aataccatgg atacttttat cttcattgta ttgatggata atatcaatca
     9121 gttcttcttg agaaatggat tcagataatc gtaaagtctc acttttaaaa ccagcttcta
     9181 atgcagagcg ttctttattt cgaacgtaca cttggctagc tggattatcc ccaacaagga
     9241 ttacagctaa accaggtatg ataccatgct gttcctttaa cctttcaact tttcgcccta
     9301 actcagcctg cattttttgt gataaggctt taccatctat taattcagtc attgatactc
     9361 cttcttcatc cgttttttct atattatatc aaaaaatagc gattatgcta ttttatatcg
     9421 ctattcattt tgtatcaatt ttaatcaata gaaaaggcaa tgaatccctc atcgcctttt
     9481 tacatatgac ttatttagta ctattcattt tagcacgaca agccgactca attgcattaa
     9541 gtttcgataa actatctgct tctgtacaac caatcgtata aaggtaacat tttatcttag
     9601 gttctgtccc tgacggcctt aaagcatacc atgaaccctc attaaaataa tattttaaac
     9661 aattttgctt tggaaaatcc ttataaccat ccttgaaatc aatagaattc tccaatgtca
     9721 tctcacctac ttgtaatatt gggttctgtc taaaatcctc cataatacga ctaatacgtt
     9781 cttgcccctc agcaccctct aactcaagag aaaattggcg ctcgttgtaa tagccaaatt
     9841 catcgtaaat ggtttgcaaa acgtctaaaa gtgtttgccc tcgttcttta taataggcag
     9901 tcatttctac taccatcatt gaagcactca cagcatcttt atcacgtaca aaagtgccat
     9961 agcaaaaacc aatactttct tcatagccaa agagataagt tttatccttt gagatatcat
    10021 attcattagc ttttccacaa atatttttaa atcctgttaa agtttcaaca gtttcaatat
    10081 tatatttatc tgcaataact tttgatagat caccagttac aatggatttt accaatacag
    10141 gatgatgtgg caaattccct aaggcacatc gttgtgaaaa aatataatag gaaagaagtg
    10201 cccctatctt attaccattt aagaaaatat attctccctt actatctttg acttccaatg
    10261 ctactcggtc acaatctgga tctgtggcaa gtaagacatc tgcatcaact gactttccta
    10321 gagattctga ataggcaaat gctttaggaa cttcagggtt tggatagcca actgttgtaa
    10381 aatcaggatc gggcatttcc tgctcaggta ctacataaat attttcaaaa ccacggcgtc
    10441 ttaaaacttc gcgcacaggt aaatttccta cgccatttaa aggagtataa actactcgga
    10501 ctgacttatc aatgttagta tcattaatag ttaaaccaag cacttctttt ttatatgctt
    10561 cttcaatact ctcatcaata taacttgcca aaccacttgc cagagcctct tcaaagggta
    10621 tttgcttaat ttgctgataa tcggttatag catccatatg attggcaatt tggtcagcaa
    10681 tatcatctaa aatctgagat ccttctttcc aatatgcctt ataaccatta taagcttgag
    10741 gattatgact agcagtaacc atcacacccg aaacacatcc tagagcacga atagcatatg
    10801 agcacattgg tgttgggcga atccctttat aaatataaga cttaatacca tttgctgcca
    10861 taatggaaca agttaattct gcaaattcct tagattgata acggacatca taactaactg
    10921 caattccacg tgcaatagct tcagggccat gatcaataat cgtattagct aatgcttgtg
    10981 ctgctttccc caccatataa gtattcatac gattggttcc tgctccaagt ttacctctca
    11041 atcccgccgt tccaaattct aatgttttgt aaaaacgatc ctgaatttca gattcatctc
    11101 ctttaatagc ttctaaatct gacttaatat ctttaccgag tgagtcgttt tctaaccact
    11161 cttgataaat ctctttataa ttcatatgag acatcctatt ctaagaccaa aatggtaaaa
    11221 atcagctcca tgataagaga ttttagttct tcaggagcta actgaatagt atcaaattaa
    11281 ccttataaag tcaacttatt ttataaatta actacactta atatatttaa agcatctaaa
    11341 tttttttcat caccctatca ttcttaatct acattatata tagacttacc aacttccaaa
    11401 caatacagta acaatttact cataacaatg gaataataaa agtctaaaac aagttgtttt
    11461 agacaatgaa attataatac tttacttaag aagtctcttg tgcggatttc ttttgtctgc
    11521 tcaaatactt tcttaggggt cccttgctca acaataatcc ctgcatccat aaaaatgaca
    11581 cgatccgcta cttcacgtgc aaaacccatt tcatgagtga caataaccat cgtcatacca
    11641 gatttagcta aatcttgcat aacagtcaag acttcaccta ccatttcagg atcaagagct
    11701 gaagtaggtt catcaaaaag aaggacatca ggattcattg caagacctct tgcaatagca
    11761 atccgttgtt gttgtcctcc agataagctt gctggataag cattagcctt ctctttgagt
    11821 ccaacttttt caagtagctc gtatgctttt gtctgagcat caagcttaga aagtcccttt
    11881 gtcttaatag gtgataaagt aatattttct agtacagtca tattgggaaa tagattgaac
    11941 tgttgaaaaa ccatgcccat tttttcgcgc attttaaaaa tatcattctt tttgtctgtt
    12001 atatcaatcc cttcaaaagt cactgttccc tttgttggta cttccaagag attcattgtt
    12061 cttaaaaatg ttgacttacc agagccagaa gggccaataa taaccaccac ttctccttga
    12121 tgaatatcca agtcaatgcc ttttaaaacc tcatttttcc caaaactttt atgaagtcct
    12181 tgtattgaaa taattggctc aaccatctat ttttacccct ttcccaagat atttctccat
    12241 ttgttttaac aaagctgaga gaatcgttgt caacattaaa tagtaaaatg ctgcaaataa
    12301 taacggtgca actggtgagt aagtagccgt tacaactgat tgtgctccgt tccataattc
    12361 catgacacca atagtttgaa ggagagcact atctttaata attgtaataa attcattccc
    12421 tagagcaggt aaaatatttt taaaagcttg gggtaagata acatagcgaa gtgtattttt
    12481 aggtcgaatc cccaacgagt aagctgcttc tatttgtcca gatggtacag cctcaatccc
    12541 tgcacgtaca atttccgaaa tataggcacc actatttaag gaaatgataa tgataccagg
    12601 taaaagtcgt gtaaaatcta aatctaaaac accaaagcta attgttggta aattgttaaa
    12661 atgcatccat gcgaaagcaa tcataatttg aactaccatc ggtgtcccac gaaataccca
    12721 tacatagaaa ttagctaata ttgtgagaaa atgtaaatta gtacgcttta ctaaagcaat
    12781 taacacgcct ataatagttc caaaaaaaac aacacatgtt gaaatcataa tggttaccat
    12841 cacaccataa ttaaaatagg accaatattg tggtaaaaat gaaaaattca ttgacatctc
    12901 ctaaaaaagt aattgccaat agtctatcac acaaagaata aaaatacaag tttaaataaa
    12961 tatttttgtt tttattctat tttaatgtat atttagatat aaaaaaagac cttcaaggtc
    13021 ttttataatt atcagtataa taaatcgtaa atttccattg cgataaggtc aatgctgtca
    13081 aatgaatatg attcacgcgc ttctacatca cgtaatgtga aaacaggacc gttttcaggg
    13141 tcattactaa aagatacttc tgctacaaca acaccttcac gttcaaaatt acgttttaaa
    13201 ttaccaccat cagtcgccat taattcgagg cgattgatga ttctcactaa atgtgattcc
    13261 atcttactta ctcctatata ttttctagtc ttttctattt taacacaaaa gtgaccaaag
    13321 caacatgaaa agcaacattt ttactaactt tttaacgatt ttatcgtcta aaatccgcaa
    13381 ataacgattt ttctcagtag tcatctattt ttcaatttag cactctaata tattcagtgc
    13441 taaaaacaga acttttttct tgatttctat gctttaggtg ctataataaa agcataaagt
    13501 ttgactatat ttgactatat acacatcaat agttaaatgt cataagaaag gggtagccta
    13561 tgctctgtca aaactgcaaa ctaaacgaat caactattca tttgtataca aatgttaatg
    13621 gaaaacaaaa gcaagttgat ctttgtcaaa attgctacca aattattaaa actgacccta
    13681 ataatccact tttttcaggt ttaaatcatg tttcacatgc gcctggtggc atcaatcctt
    13741 tctttgatga tttctttgga gatttaaata atttcagagc ctttaatggc caagatttac
    13801 caaacactcc tcctacacaa gcaggaggaa accgaggtgg aggaaacggt aatggacgaa
    13861 ataataatcg caaccaaact gcaactcctc ctcaagccaa agggattcta gaagaatttg
    13921 gtatcaatgt cactgaaata gcccgccatg gtgatattga ccctgttatt ggacgtgact
    13981 cagaaattat tcgtgttatc gaaatcctta atcgtcgtac aaaaaataat cctgtactta
    14041 ttggcgaacc cggcgtaggg aaaactgccg ttgttgaagg acttgctcag aaaattgttg
    14101 atggtaatgt gcctcataaa cttcaaggca aacaagttat tcgtttggat gttgtgagtc
    14161 tagtacaagg aacgggcatt cgtggtcaat ttgaagaacg catgcaaaag ttgatggaag
    14221 aaattcgtca acgtcaagat gttattctat tcatagatga aattcacgaa atagttggag
    14281 ctggtacagc tggcgaaggt agtatggatg caggtaatat tttaaaacct gcccttgcac
    14341 gtggagaatt acaactagta ggtgccacta ctctcaatga atatcgtatt atcgaaaagg
    14401 acgctgcctt agaacgccgt atgcaacctg taaaagtcga tgaaccttct gttgaagaaa
    14461 ccattactat tttgaaaggt atccaaaaaa aatacgaaga ttatcatcac gtaaaatata
    14521 ataatgatgc catagaagca gctgcagtac tatctaatcg ttatatccaa gaccgctttt
    14581 tacctgataa agcaatagac ttattagatg aagctggttc taaaatgaac ctaacactaa
    14641 attttgttga tccaaaagaa attgatcaac gtctcattga agcagaaaat ttaaaagcgc
    14701 aagcgactcg tgaagaagat tacgaacgtg cagcttactt ccgtgaccag attgcaaaat
    14761 ataaagaaat gcagcaacaa aaggtcgacg atcaagatac acctattatt accgaaaaaa
    14821 caattgagca catcattgaa gaaaaaacga atatccctgt tggtgattta aaagaaaaag
    14881 aacaatctca attaattaat ctcgcagatg acttgaaaca gcatgtgatc ggccaggatg
    14941 acgctgtcat taagattgca aaagctattc gtcgtaatcg agttggtctt ggtagcccaa
    15001 accgtcctat tggttccttt ttatttgtag gaccaaccgg tgttggtaaa actgaacttt
    15061 ctaaacaact agcaattgag ctctttggtt cagctgatag tatgattcgt tttgatatgt
    15121 cagagtacat ggaaaagcat gctgttgcta aattagtcgg agcgcctcca ggatacgtgg
    15181 gatacgagga agctggacaa ctaactgaaa aggttcgtcg aaatccttac tcgctcatcc
    15241 ttctagatga aattgaaaaa gctcatcccg atgtcatgca tatgttcttg caggtccttg
    15301 atgacggtcg attaacagat ggacaaggaa gaactgttag ttttaaagat accattatca
    15361 tcatgacctc aaatgctggt tctggtaaaa ctgaagcaag tgttggcttt ggtgcctcac
    15421 gagaaggtag gacgaattcc gtactaggtc aactaggtaa cttcttcagc cctgaattta
    15481 tgaaccgctt tgacggtatt attgaattca aggctttaga taaagagaat ctcctcaata
    15541 tcgttgatat tatgttatct gacgttaatg cacgtctcgc catcaatggt attcatctag
    15601 atgtcactga taaagtgaaa gaaaaattgg ttgatttagg ttacgatccc aaaatgggag
    15661 cacgtccatt acgtcgtacc attcaagaac atattgaaga tgctatcaca gattactatc
    15721 ttgagaatcc aagcgaaaaa gaacttcgtg ctattatgac tagcaatgga aatatcataa
    15781 taaaatcttc taaaaaaact gaggaaagta caaaaggttg agtaagaaac tcaacctttt
    15841 ttcacactct tctcaaaaaa taatagcctc tataaagctg tttctctcct atctcgtctt
    15901 taaattttaa tacaattatt aaggaaatat tccaaaaatt ttagtataat aaataaaaaa
    15961 taattaaagc ttttaaaatc ataaaacaca atcaggagag agaactatga ctaatcctac
    16021 tttcggtgaa aaaatagata atgtagacta taggtcacga tttggtgtct atgctattat
    16081 tccaaatcca actcatgata aaattatttt agtacaggca cctaatggtg cttggtttct
    16141 tccaggtggg gaaattgaag aaaatgaaaa tcacctagaa gctttaactc gtgagttaat
    16201 tgaagaatta ggttattctg caacaattgg ccattactat ggtcaagctg atgaatattt
    16261 ttactctaga caccgtgata cttactacta taatcccgcc tatatttatg aagttactgc
    16321 ctatcataag gagcaagcgc cactagaaga tttcaaccat ctagcctggt ttcctatcca
    16381 agaagctaaa gaaaagctaa aacgaggaag ccatagatgg ggtgtccaag cttgggaaaa
    16441 aaatcaccat tctaggaaat aattcatacc tttcttccta aatagtgtta taataaacct
    16501 aataagaatc agggggaaaa tagttatgag actaattaat acaaccagta gtcatcctga
    16561 actagttcga aatcaactcc aaaatacaga tgcaaaatta gtggaagtct attcagctgg
    16621 caacaccgat gtcgttttta caacagcacc taaacattat gaattattaa tttcaaacaa
    16681 atatcgtgct atcaaagatg aagaattaga agctattcgt gagttcttct taaagcgtaa
    16741 aatagatcaa tctattatta ttcaagagca gatgaaatca ctccatactg ccaagttaat
    16801 tgaaatttca tatccaacaa cagcataatt aaaagaagcc taatcaaggc ttcttttttg
    16861 atgactacta aacaaatata gcattaaata atttttatta agcttcaaaa ccttgagcta
    16921 ctgcttcagg ataatattgc tcaataattg ctgcactagc atcacaaaca gctacaccat
    16981 acgaacgcat ccttgtggtt ggatcaatac ggcgtgagcg ttcgcagact tcaccttcag
    17041 cgtgttcaac agtaaatgca acaccttcaa atgatactga gtcagctggt ttgtctgctt
    17101 catcagcaat tgttaattgt gagacaatca tcaagagtgc aatatcgcta tttaacgctg
    17161 ttaataacgt tttcacttct tgactagcat aaattgttaa atgtgcttct agagatttac
    17221 caatgacttt agcgttacgt gcttcttcga gagctttttg agcttgtgtt cgtaacgtca
    17281 tgaatgctga ccactcttca agaatttctt cttgaccaga gaatgtctgt gcaacaggca
    17341 tttctgctaa ttgaacaaat tcctcctcct catgttcaag gtatgaccaa atttcttcag
    17401 cagtatgtgg tagaatcgga gtcaaaagtt ttgtaagttt aactaaaata tcgtagaaaa
    17461 cagtttgcat acgacgtcgt tctggactgt tagccgcttc aatgtaaacc acatcttttg
    17521 caaaatcaag gtaaaatgct gatagatcaa ctgttacgaa gttaacgaca gctttataaa
    17581 ttgccataaa atcataagca gcataagctt tgttgattgt atccacaact tgattaaatt
    17641 taatagtcat ataacgatca acggcaccta aattttcata ggctactgca tcttgtttag
    17701 gattaaaatc ggaagtattt gcaatcaaga aacgcaatgt atttctgatc ttacgataag
    17761 tttctgatgt ttgttttaag atatccattg aaatacgaac atcattactt gaatcaacag
    17821 aagttaccca cagacgcaag atttccgcac caaattgttt ttcaacatca ctcggaagaa
    17881 ttgtatttcc tagagattta gacattttct cacctttacc atcaagtaca aaaccttgag
    17941 ataagactgc tttgtatgga gcgtgaccat taacagcaac tgatgtaatt agtgatgagt
    18001 tgaaccaacc acggtattga tcagaacctt caagataaag atctgctgga taggaaaggt
    18061 tttcacgagc attcattact ccattccatg atgaaccaga atcaaaccaa acatccatga
    18121 tgtctgtttc tttctcaaag agaccattag gtgaacctgg atgagtataa cctgctggta
    18181 gaaggtcctt agcatcacgt tgccaccaaa cgattgagcc gtattcagca aataaatctg
    18241 caacgtgatc agtcacttct tttgtcatga tagctgtacc atcttctgca taaaaaattg
    18301 gaagtggaac accccaagca cgttgacgtg agattaccca gtcgccacgg tcacggatca
    18361 tgttataaag acgtttttta ccccactcag gttggaaatt tgtcttttca atttcatcga
    18421 ggatttcttg acggaatttc gaaacagagg caaaccattg tggaaccgca cgccaaatga
    18481 ttggcttttt cgtacgccag tcaaatggat atgagtggtt gataacttcg ctggctaaaa
    18541 gaagatctcc caatttttct tttacaagtg gtgtcacttt gtcatagaat tgaccttcaa
    18601 aatcaggacc agcattttcc atcatcaaac cacgactatc tacggtaact acaacatcaa
    18661 gtccattagc aataccaacg ttatagtcat cttcaccaaa accaggagcc gtgtggacaa
    18721 tacctgtacc agaatctgtt gtaacatggt ctccaaggat aaccaactct tctacttctg
    18781 tatcccatgg atgttctgta acaatgtgat taagttcttt accagtgtga tgagtcacaa
    18841 tttcaaagtt ttcccagcca aacttagcag cgagactatc tacaagaacc tctgcaagaa
    18901 ggtatttacg ctcactacct actggtgcaa ctacaacata ctccatatct ggtcctactg
    18961 ttaaaccgcg tgaagctgtt acagtaaatg gtgttgtcgt ccaaacgacg atataggtat
    19021 ctgtatcaag aattccctta ccatctttaa ctttattggc atagtagagt gatgtcgaat
    19081 caatatcatg atattcgatt tcagcctcag caagggcaga ctctgatgac catgaccaat
    19141 acactggttt agcaccacga tagatatatc ctttatctgc catagcaccg aaaacacgta
    19201 cttgatctgc ttcataatct ggtgtcagtg taatataagg attttcccaa tcagcagaaa
    19261 cgcccagacg tttaaaatca tcacgttgtt tgtcaacttg gctgagagca taatcacgac
    19321 acatttcaag gtattcagcc aagtccattt ctttacgttt aacccctttt ttagctaata
    19381 cttgctcaat tgggagtcca tgagtatccc aaccaggcac ataaggagct cgaaaacctg
    19441 acattgattt tgaacgtaca ataatgtctt tagaaatttt atttagtgca tgtcctacat
    19501 gaatattccc attagcatag ggaggtccgt catgaaggtg aaaggctggt tttccttcat
    19561 tcaatgcttg acgtttttta taaatatcag cttgatccca tgcttcttgc cattgaggtt
    19621 ccttatttgg aagccctgca cgcattggaa aagctgtttg tcctaaattc aaggtttctt
    19681 ttaatttcat actaactcct tatctcatag taatagttat cacaattttt taaattgcta
    19741 aataaaaaag ctttcatcac ataaaggacg aaagctcgtg gtaccacctt aattcagagc
    19801 agaaaatagc tatttcttac tccctcaaca acccgtaagg tgggttaagc cttctacctt
    19861 acttaaataa tagttcaggt agaatgattg aaaatgataa tttcaatatt agggattgta
    19921 gggctctcac catctcctac tcgctgataa tataatatta aaaccttgtt tttcttaatc
    19981 ttcaatattt aatttaaaag tttgagtttc acctaaattg atcggttctt ccatttggaa
    20041 attattagta tctaataaac ctgactcttc aagttgtttg ttactttctt caacacgacg
    20101 ctgtaattct tccatttcat ctggtgagaa ttgacgagta gcatcaaatg actcagtgtc
    20161 atccaccact ggtaaagcat catcttcatc aagaactttt tcaacaactt ctttgaaaga
    20221 agcatcagaa ttttgaagat aaatagctgt tggtttcaac aattcttccc aagcagatga
    20281 gttagctaat tttaactgac cttctaattc agaaagtaag cgttggtgga aaacacgtga
    20341 ttgacgtttc aaatcttctg tttctatagc gacacgctta gcatcatctg tcgcatcacg
    20401 aaggatttga tttgctttta atttagcctc atcaattaaa tgttgagcat caaatgtagc
    20461 tttccccatt aggttagatg cttcatcctg tgctgaaatt ttcacacgtt cagcagtttc
    20521 ttgagctaaa ataactgatt gacttaacga ttccttcatt tcgttgaagt aagcgatttt
    20581 ctcttctaaa tctttaatgt attgctcttg ctcacgatta cgtctaatca agtcttcgta
    20641 atcgtcaaca acaatctcta aaaattcatt aacttcttct tcgctataac cgcgaaattt
    20701 tgatgaaaat gttttatctt taatttcaag tgctgtaagt ggcatgtttt ctccttagtt
    20761 atgtatcatt ttgtctactt ctaacttata tttttgattt ttagttaacc ctaggttatg
    20821 gttcaacgta aatcgaccat atcctctaac actaatcaaa tccccctcta ctaattgttc
    20881 agaaactcga ttgacgattg cataattgac cttgacttta tctgcctcaa tcaatttcgt
    20941 cgactgtgtt cgtgaaatct ttaaaatcgt tgctaaaatt ttatcaagac gtaaactgga
    21001 agcaataaca gttagttttt gaatatcttg tttgggagtt agaagtttgg acaagggaac
    21061 ttcagctaat tgtacactag cagttccaat ttttgtaact gaatgaacaa ggtgatttgt
    21121 catttgacta tcgaccaaaa cctgagcaca gccttcttca actaggatat cccctaaaat
    21181 tgatcgtttt acacctaagt aattcagtaa agttcccata attttagcat gggtaaggtg
    21241 attaaattta gcattatact ttatttcaat taggctaagg ttaaaatcaa ccatatctaa
    21301 ctgatagtat tccggtgcta ttattacttt aacatattct gtttgaaaat actgaccaga
    21361 agtgtaataa tgactacctc gcctctctag aacactttcg agtattttaa attccctagg
    21421 atttagaaat tcagtggtaa taaacgaata ggtgttttcg acatattgag ctaaatggtc
    21481 tattttattg ataaacgcat actcttctgg tcgaaaatgc tgataaatat catctaacgt
    21541 catcttatgt aaaaagcaag attaaaaatc tactcaacat atttagtaaa aagaatgcta
    21601 ctattactgt aaaatctaga cctaaaaatt gtagattaaa tcttctgagt gggcgaataa
    21661 tcggatcaac aatctgtacc actagtctac caatccatga atcgtatgct cccggaagcc
    21721 aagataacaa ggcataggct agtaagatat aagaatagac cctaattgtt tttaataata
    21781 ttaatagtac taatatcata tttatcgtct cttcatatca aaatcaaaac tcgtctcttg
    21841 accagtcttt ggaatattca tctcctcaat atcgaccata acattggctg gtgttaatag
    21901 aaacattgaa ctcccaacct tttgtaatga accatataat actctacttg caccatcaat
    21961 ataatccaag caacgtcgtg cttgcgcatc aagcatatat tgaaaatcaa tcaagacaca
    22021 ttcattaacg ataagtaaat caacaatttc ttgtgcatcc tcatacttac ggggatactt
    22081 cagagcaatt gttacttgtt ctctctgttg tcgaactgag tctttatttt gaatcctagg
    22141 agtagcttgc tgataagcat tgtctctctg taccctttgt ctctcctgac gattaggatt
    22201 ataggtagaa gttctcggac gactaccaat catctcttcc tctgcggagt tagtcatatg
    22261 actacgctga gaagcttcct gtgctgtagc tgctctggaa tctctttgca ctgaagttct
    22321 ctcttgtact tcgtgtactt cattttcact tacatcgtca gtgtcaaaat aagaaataat
    22381 tttgtcaaat ctatctttta atgccatatt cccctccatt atttaaagaa tgaagttcct
    22441 atccttacaa atgttgatcc attttgaata gcaatatcat aatctctact catgcccatg
    22501 cttaattctg taaacggcat tcgttctatg ttcctagctt gaagcctttt cctaagactc
    22561 tcagtctctt caaaaattga tgatatttcc tctttagttg cttttaaggg agccatagtc
    22621 atgatgccga taagttcaat tttatcgtac cgagaaatta agttcagtgc atcatctatt
    22681 tgctcaatag taaaaccatg cttactgtct tctcgtgata tattaacttg tagaaaacat
    22741 ttaactggtt tttgagcacg tttttgaatc tcagctgcta acttaacaga atctaaagca
    22801 tgaaaataat caacataatt aatgacatct ttaacttttc gacgttgtaa actaccaatg
    22861 agatgccatg taagcttttc atcttttaat gcttgatatt tttcaagaaa tttatcaaca
    22921 cgattttcac cgatatgatt aacacctgtt ctaataagcg cttctgttgt ttgacagtta
    22981 acatattttg ttacagctac gatatgaact gattcaggag cgcgacctgc ttttagtgct
    23041 aatttactaa cattgtcaaa aatcgctgtt ttattttctt gtaaattcat cattttaatt
    23101 aacggttttt aaagaatgga ggtgtttcaa gttcatcatc catatcatca ttttctgaaa
    23161 acgttgacat agacaacttg ctatcaagtt caccttctgt cggacgtgaa atattatcac
    23221 gacgaaggtc ccaattacca aaagcagaac tttgttgctg attttgtgca tgaggctgat
    23281 tttgttgagt tggcatttcg cgagactctg tcatatcaaa gttgccacga cgatcaaagt
    23341 ttgaatttga agtactttga cgttctgaag gtgcttgatt agttggagct gatgttgtaa
    23401 aaccagatac ttgattagtt ttatctttac gcacacctgt tgcaacaact gttacgcgga
    23461 tttcgtcttt catatccatg tcaattgaag tgcctaacca aatattgaca cctttacctg
    23521 ctgcttggct aacaatctct gatgcttcct ctgcttcagt aagtgtcata tccataccgc
    23581 cggtaacatt gacaataacg tcttctgctc catcaatcgt tgtttcaaga agtggtgaat
    23641 aaattgcttt tcgagcagct tcagtaatac gttcctcacc agaaccgata ccaattccca
    23701 tcagtgcatt acctttattt gccataactg ttttaacatc tgcaaagtca aggttaatta
    23761 aacctgggtt agtaatcaaa tctgtaatac cttgaacacc ttgacgtaaa acattatctg
    23821 cttcactaag agcttcaagc aatggtgtct tcttatctac aatttcaaga agattattat
    23881 ttgaaatgat aagcagtgta tcaacttgtt ctcttaattc ttgaatacct tcgatagcga
    23941 agttagaacg tttattaccc tcaaagccaa aaggacgtgt gataaccgct actgtaagtg
    24001 ctcctaaact ctttgcaata cgtgcaataa ctggagccgc acctgtacca gatccgcccc
    24061 ccatacctgc tgtgataaat accatatcag cgccagtaag tgcttctgtt aaaacttctt
    24121 cactttcttc agctgcttta cgaccaactt caggttgtcc tcctgcacca agtccgcgag
    24181 ttaattttgg accaagttga atgacagttt cagcttttga gctacttaag gcttgaatat
    24241 cagtatttgc tgcaataaac tcaacgccag cgacaccttc gtcaatcatg cggttaatag
    24301 cgttaccgcc accgcctcca acaccaataa ctttaataac tgcaccttgt actgatgctg
    24361 tatcaaatga aaataccatt tttattcctc acttattgtt ttagtcaaac atacttccaa
    24421 aaattcctct gatgcgctca ccaatattag ttttaggctc agtaggctct tgagcgtttt
    24481 ctcttgctcg gattggttct aaattagaat cttccataga agaggttaac ggctcagaat
    24541 aaggcattgt cgacattgta tttgtttttt ctttataatc aaagtcgact ggtttatgtc
    24601 ttagcatttc gtcgccagtt acagcatgtt gtgcgataat gtcaacttct gacatcattc
    24661 ctacataatc aacgatacta ataacattag cgaacattgg attgcgaata cctacttggt
    24721 ttggaacgtg caatttaact ctagttccaa aaatttgttg agcaacctcg acaactcctg
    24781 gcataattgc accacctcca acaagaataa taccaccagg taaatcaagt aaacgacctc
    24841 tacccaagtc ttgtttgaca tgttctaaaa tttgacgaat acgtcctgaa ataatttgcg
    24901 aaagatagct ttctgtaatt tcaacaggtt cctcgttacc aacaacatta acttgaactg
    24961 tatcagaagt acttgcttct tcaacattag cttgtccaaa gttgaatttc aatgcttcgg
    25021 caatttctac tgttgtacgt aacactttag aaatatcttt ggtgacataa tcgctacctt
    25081 cagaataaat attagtatat tgcaattctt gattacgcat agaagcgact gttgtttgtc
    25141 cgcctcccat atcaatcaca gttgcaccaa actcacgttc accttcgttt agaactgatt
    25201 ttgctaaggc tagaggggca atcacaacat gctctacttt tatacctgct cgttcaacag
    25261 ttttacgaag attatgaagg atagttgttg gtccagtata gataagacca cgcatctcaa
    25321 ggcgaatccc catcatccct ctagggtctc taatcccttg gaaaccatct acaatgaatt
    25381 caagcggaat caatgaaata acttctcttt caggagtaat gcttttagtc aatgctgatt
    25441 taacaacact ttcgacatct tcatccttaa tttcttttga ttcattggga acaggaatca
    25501 ttccttgagt tggttcaatt tggagaagat ttgctggtaa tccaacattg attttgtcaa
    25561 tggtgatgcc tgctttctcc tcagcttgtt ttaccgcctc tttgattgca gttgctgctg
    25621 cctcaatatc aataattata ccatctttta caccggaact agggacgttg ctaaccccaa
    25681 ttacattcat ttcatttgca ataaattctg caactaaaac tttaatcgag cttgttccta
    25741 tatccaaacc agtaaaaaag ccatttctag ccattcactc gacctcgcta tctttccatt
    25801 cttttctatt ctacatctgc caaaaacaga attattcaga aaatattata ccataaaaaa
    25861 agcagaattg ttaccaaatt acgttttttt attaagcaac atcaaattga aatatttcta
    25921 acttatttaa accattcgca acttataaca aacttaaatt tttttcaaga aatcaatttt
    25981 tactagcaat ctatgactga cttgatatgt ataaaaaaga ctgataaatc cagtcttttt
    26041 ttatcgctaa ttaacatttt gagggttggg tgcctgctct gttgctatct gttgtccttg
    26101 ttgattagta tttgagttat ttgtttgtcc ttgactattt tccgcaacct gaccattttg
    26161 tgtttgtgtt ttatcagttg atttattttt tgtatcttcc gctttcacag gggttgattc
    26221 aatagtactt gttgttgtgt aaactcccac ttccatatca acaatagaag gttccttaag
    26281 gttcttctta atttgtttgt aaaaaggaag tctttcttta aatttagata atggtattct
    26341 aatactattt ccatcatgca tatctaacag caggaggtca ggtgtcgttt tagaatcagc
    26401 taaacttatc acctgaatct cacttattaa atcagggtct aaagccttta aatctttaat
    26461 taatagctta atactatctt ccttatcaag gttaattgtt aagaagtgct ttggtagctc
    26521 tgaactattt acaggatcag ccttttttcc agtttccaag acaggctgat atccttgctt
    26581 tgtatgtgca tatgcaataa tcttattttc ttgaacttga atatgaaact tattgggaaa
    26641 ttgataagtc atctgagctg tttttaccca tacatcttct gcagctaaac gttgttcaat
    26701 agctttatgt ttaaaaatta aagaaaagaa ataatcgttt ttttgaatat tcg
//
LOCUS       DATRUK010000021        26462 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.021, whole genome shotgun sequence.
ACCESSION   DATRUK010000021 DATRUK010000000
VERSION     DATRUK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26462
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.021"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..648
                     /locus_tag="VBE34_000880"
     CDS             <1..648
                     /locus_tag="VBE34_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7263609.1"
                     /translation="TFEKPVQLLESICEKGMHNLYFLDIEIKNDEQRGLEVAKQIRQI
                     DPYAQIVFVTTHSELMPLTFRYQVSALDYIDKGLSQEAFSRRIEDVLLYANNACSKNL
                     LEDSFYFKSRYSQVQVPFNDILYIETSLRSHRVVLYTAKDRMEFTATLGDILKQEPRL
                     FQCHRSFLVNPLNIFKVDRSNKLIYLKNGSNCLISRNKIKDIMVVIEENQRGRGI"
     gene            645..1979
                     /locus_tag="VBE34_000881"
     CDS             645..1979
                     /locus_tag="VBE34_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO7263610.1"
                     /translation="MNSTLSILEIALTIILTIFLFSKISGRQLTLIEIGICIALRLGL
                     VILFIAANTIINLSFLKYWLLPIYMLSLAFLLLRPISKTLVVFYGLFPIVLRNLCSRS
                     LAFFVFPLFNIDFHTTNQGFWGYFVDVLSTLCVLLFLRWMQYDFKTLRSSEVSPIDKR
                     ILQFTNGCMVSYYLLMQFSTYLEYELAINTVIYRQFLLVVYWIIFTGAIASLDRRLRN
                     SLQERLAVQRELQLANMIDYSHHIEGLYKEVRSFRHDYSNMLTTLKLGIDNEDISIVK
                     SVYQSIFKESNTHFRHPKYNIDRLVNVDNPALKSLLAAKLSQAIDNHIMVSIEVPDSI
                     KPQGMELIDFITIVSVLWDNAIEASLETDDPKIAMSYFTLKHKQIFIIENSTKEKAID
                     TSTIFNYEISSKGRNRGIGLYNVRGVINRYPNISIATSSQDFSFCQTFEIIL"
     gene            complement(2050..2232)
                     /locus_tag="VBE34_000882"
     CDS             complement(2050..2232)
                     /locus_tag="VBE34_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7263611.1"
                     /translation="MLTYKKALVRQVDTKDCGVTALASIAKFYGSKYLLNHLRELAKT
                     NRDGTTASGIIAGTTK"
     gene            2521..2694
                     /locus_tag="VBE34_000883"
     CDS             2521..2694
                     /locus_tag="VBE34_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO7263612.1"
                     /translation="MKHYHMLKVALVIDGYTVRTGKHTYTSAWHLSENTAAANARNRM
                     GLYPGSVCRVYLV"
     gene            2763..3611
                     /locus_tag="VBE34_000884"
     CDS             2763..3611
                     /locus_tag="VBE34_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7263613.1"
                     /translation="MLLQLDNITKSYGKKIVLNQISYQFTPGLYGLLGANGTGKTTLL
                     NLMSHFTFADSGNIYWNGQEQSEEFYRHIGFLPQHFRYYDQFTGIAFLNYIATLKGVD
                     KKKAKPEIPRLLELVGLGDVGKKKISSYSGGMKQRLGIAQALINDPEILILDEPTVGL
                     DPKERVKFRHILSQLSTNKIIILSTHIVSDVEAVAKEIIVLKNGKFIEQGNTAQLLKT
                     IEGKVWEITTEPDLSQIPNIAIVNEKVISDSLVFRVVSDICPSDSAQLVVPTLEDFYI
                     YHFRGE"
     gene            3617..4771
                     /locus_tag="VBE34_000885"
     CDS             3617..4771
                     /locus_tag="VBE34_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEO7263614.1"
                     /translation="MISIFPFELKKLLQRKAALGAFIIFLLAIFGLFYQHFFVGQISG
                     YSADKIHGREAVAINRRIAEKYSGDLSEERVTNIIKNYVNPPAGRPPIFDVFSYYVAD
                     TFDRDFLIYYNQLKDDKTIDFNKINIKDQKQLGSALPLKQLKLGNFAPWNQLFEVSNS
                     TYILIVLFTLFLSAPLFSGDRAKKINPILLTTRYGRGQLTWAKLASTMTIGISTFILA
                     YALILTVFADYFGFSGWETSVQLNLYWTSPLANIMGFPVPMTISTAFVHIIVLQFVGL
                     LFIASLNFFILSITNSPLTSFAVSTLAFFAPSFLMAMFNKGVINKLLTIFSVSTTDTS
                     ALLLKLSRNGSHGFFFDSFSANEICLISLRMLIAVLLIAYTYRLMRRRSL"
     gene            complement(4766..4948)
                     /locus_tag="VBE34_000886"
     CDS             complement(4766..4948)
                     /locus_tag="VBE34_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263615.1"
                     /translation="MIPISSEEYLNYPQKSLKRLKWYLIIIVNTLSTILGNKKADFAK
                     ENQLRIISCSDNQWEL"
     gene            5000..6034
                     /locus_tag="VBE34_000887"
     CDS             5000..6034
                     /locus_tag="VBE34_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO7263616.1"
                     /translation="MDNKQHNTSMISLLQYLFSILVILVHSGRLFSQDVIHFTFKSFL
                     GRMAVPYFLICTAFFLRGRIQQGLCNHSYFRKLIKKYSMWTIIYLPYGYFFFESLNIA
                     KIYLLPGFIVAFLYLGMSHTLWYIPAVILGWFIIQGLLKYVGTRGTFITVVVLYCIGA
                     VETYSVFVQSTKFYPLMSTYMSIFQTTRNGLFYTPVYLLAGYLLYDYFNTDLFTKSRG
                     LKYILFLLLLALENVLIYFNQGLDKNFFLLAPLCAVFLFNWSIRTSLFKGYRLSPLKQ
                     LSVYYFFLHPLFVGIVSYCLKSTSLVAHHQGKVIFVVTLALTHACALLIIKVVNKIPY
                     QKIIPLSLSK"
     gene            6281..6706
                     /locus_tag="VBE34_000888"
     CDS             6281..6706
                     /locus_tag="VBE34_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEO7263617.1"
                     /translation="MKRKFLIGSHGKLASGLQSSIDILTGKGQEIQTIDAYIDDSDYT
                     KSIVEFIDEIAPDEQGLIFTDLLGGSVNQKMATAVMNSGKNNVFLITNSNLATLLSLL
                     FLKPEEELTKEEIVTVINESQVQLVDLSFKACSEDDFFD"
     gene            6724..7227
                     /locus_tag="VBE34_000889"
     CDS             6724..7227
                     /locus_tag="VBE34_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7263618.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEESIALFKDPRATDKRIFVIVNSVKDACTIAKNITDLEAVN
                     VANVGRFDKSDPATKVKLTSSLLLNPEELEAAKELASLPDLDVFNQVLPSNTKVNLSQ
                     LVKQSEV"
     gene            7231..8040
                     /locus_tag="VBE34_000890"
     CDS             7231..8040
                     /locus_tag="VBE34_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO7263619.1"
                     /translation="MIMPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGMLLGDI
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISNIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNSFVHHISQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDIELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            8037..8864
                     /locus_tag="VBE34_000891"
     CDS             8037..8864
                     /locus_tag="VBE34_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO7263620.1"
                     /translation="MKSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASTGSAMGAVVFLLLYNIPAFLIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQSYLDQLFVGVVPLLVTILAFWLLRKKVNINWIMFGIMVLGIVLGLLGIC"
     gene            9002..10651
                     /locus_tag="VBE34_000892"
     CDS             9002..10651
                     /locus_tag="VBE34_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7263621.1"
                     /translation="MRGYRMEERFKKRLQDDISKHFSRQSLILSLLLIALFVLFSLAP
                     QQIGLYKDVNSVSYSYKQLIQKHDTLLDDLGKNSLKPFVSGHLGSADLSKQYYHLRNH
                     LQSQTELLVFSPNQELLFASNSHLGNFFSKSIYISEVLDKAKINQRLLKIIVDSEGGH
                     YLALIKPIIVNKKVSGYAFLLMNGKDFLLPTKAINSDLIIADQLNNSFTFTNRDFISS
                     SLDKVDSQFLTRYFSFHDHRAFVVRKVALQDNILLYMYRPLIPVTLVVLFSLVSSVII
                     FVILRRKSRVLADRIAVKNSGAINQMVLDMDAISRQEKSSIELDSQDEFQYLSVQINQ
                     MVSRLKDLHEKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSQLTERIV
                     IQLTKLLRYSLSGSTEAAVLKDDLAIIESYLLINQVRFEELTYTISVSPELEHMRVPK
                     LFLLPLIENAIKYGLKERHDVAINIDIWQDSDGIWFTVSNNGSGISLARQKAIRTMLR
                     SNHSHHGLINSYRRLQYQFSTVLLEFTKTDDAFRVSYIVKE"
     gene            10655..11428
                     /locus_tag="VBE34_000893"
     CDS             10655..11428
                     /locus_tag="VBE34_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO7263622.1"
                     /translation="MYRLLIVEDEHLIRKWLRYAIDYQSLNILVVGEAKDGKEGAQLI
                     QEEQPDIVLSDINMPIMTAFDMFEATKGQSYAKIILSGYADFPNAQSAIHYGVLEFLT
                     KPLEKQALIDCLKTIMARIEEHKEKHLQEHTELYLPLPQANDQVPEVIKDMLAWIHSH
                     FHGKIVISQLAHDLGYSESYLYTVTKKHLHITLSDYINQYRINQAIQLMFREPDLMVY
                     QIAEAVGIYDYRYFDRVFKKYLGQTVKAFKEEHIFKQMD"
     gene            11440..12477
                     /locus_tag="VBE34_000894"
     CDS             11440..12477
                     /locus_tag="VBE34_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO7263623.1"
                     /translation="MKEKQSKRLIYILLIVPIIFISVFTYSISQPSKLLPPKELVILS
                     PNSQAILTGTIPAFEEKYGIKVKLIQGGTGQLIDRLSKEGKQLKADIFFGGNYTQFES
                     HKALFESYVSKNVHTVIPDYIHPSDTATPYTINGSVLIVNNELAKGLTIKSYEDLLQP
                     SLKGKIAFADPNTSSSAFSQLTNILLAKGGYTNPKAWNYVKKLQHNINAIKSSSSSEV
                     YQSVAEGKMIVGLTYEDPSVNLQKSGANVSIVYPTEGTVFVPSSVAIIKNAPSMKEAK
                     LFINFMLSLDVQNAFGQSTSNRPIRKDAQTSNGMKALKDIATLKEDYRYVTKHKSQIL
                     KTYNRIRRNAD"
     gene            complement(12699..13196)
                     /locus_tag="VBE34_000895"
     CDS             complement(12699..13196)
                     /locus_tag="VBE34_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO7263624.1"
                     /translation="MVATLIIIRGNSASGKSTIAKQLQAELGEDTLLLSQDYLRREML
                     GTKDGENTTTIPLLINLLNYGYHNCSYIILEGILRSDWYTPVWKHILKHNPNNTYAYY
                     YDLSFQETVKRHSTRLKSLEFGEDSLARWWLEKDFLKEIPEKILTKSMSLEDVKKLIM
                     TDVLP"
     gene            complement(13196..14233)
                     /locus_tag="VBE34_000896"
     CDS             complement(13196..14233)
                     /locus_tag="VBE34_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEO7263625.1"
                     /translation="METTLNYIKTLTSIPSPTGFTQTIMTYIIKELEAFGYSPIRTNK
                     GGVMVSLKGKNNTKHRMITAHLDTLGAMVRAIKPDGRLKIDLIGGYTYNAIEGENCTI
                     HLSKNGKEISGTALIHQTSVHVYKDAGTAERNQTNMEIRLDEKVTTADETRALGIQVG
                     DFISFDPRTIITGSGFIKSRYLDDKVSAGILMELLSVYKKEDIQLPYTTHFYFSAFEE
                     LGHGANSSIPNETVEYLAVDMGAMGDDQETDEYTVSICVKDASGPYHYELRQHLVSLA
                     ENNNIPYKLDIYPYYGSDASAAMRAGAEVKHALLGAGIESSHSYERTHIDSIQATELL
                     VDAYLKSNMVN"
     gene            complement(14244..14699)
                     /gene="nrdI"
                     /locus_tag="VBE34_000897"
     CDS             complement(14244..14699)
                     /gene="nrdI"
                     /locus_tag="VBE34_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO7263626.1"
                     /translation="MPKLTLVYISLSGNTQSFVKRLSEQLSTWHNCQVSTINIKEQNH
                     QTFPVDQPFVALLPTYLEGGNGIDNGDQEILTIPLGDFIAAHNNVKNCIGIVGSGNRN
                     FNNQYCLTAKQYSERFGFPMLGDFELRGTSSDVERIANIIVETLSNFKA"
     gene            complement(14856..17258)
                     /locus_tag="VBE34_000898"
     CDS             complement(14856..17258)
                     /locus_tag="VBE34_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HEO7263627.1"
                     /translation="MSKHYFSKSIFALTVLTATTTSGLAVQAEDIVTTPSSTATKVES
                     TTPTSTIAEEKSNVTSTPTAITDASTATSTNTTANNQNPQPVATETTTGDLKPIEGEK
                     VDLRIMSTTDLHTNLVNYDYYQDKESQKIGLAKTAVLIEEAKKENPNTVLVDNGDVIQ
                     GTPLGTYKAIVKPVAENEEHPMYQAMNALGYDASTLGNHEFNYGLDYLKKIIATANLP
                     ILNANVLDFKTHQPVFKTYDIITKTFKDSTGRAVALNIGITGIVPPQILNWDKANLEG
                     KVIVKDSVKAIEEIVPTMRAKGADVILVLSHSGIGDDRYEEGEENVGYQIASIKGVDA
                     VVTGHSHAEFPSGNGTGFYEKYTGVDGINGKINGTPVTMAGKYGDHLGIIDLGLSYTN
                     GKWQVSESSAKIRKIDMNSTTADERIIALAKEAHDGTINYVRQQVGTTTAPITSYFAL
                     VKDDPSVQIVNNAQRWYVANELKGTPEANLPLLSAAAPFKAGTRGDATAYTDIPAGPV
                     AIKNVADLYLYDNVTALLKVTGADLREWLEMSAGQFNQIDPNNKAPQNIINTEYRTYN
                     FDVIDGLTYKFDITQPNKYNKDGKVVNSQASRVRDLMYNGKPVADKQEFMIVTNNYRA
                     SGTFPGAKNATMNRLLNLENRQTIINYIISEKTINPTADNNWGFTESIKGLDLRFQTA
                     DKAKNLVTNSKDIQYIASSTKDEGFGDYRFVYTEQEKVDLTEPSHQEKLSQIEKQKIH
                     HMVATAYTKAKASPNIAGTIYTQQKEKTLPKTGQEKQSYLAFFGVAILGLVGNLLKKK
                     QI"
     gene            17518..19734
                     /locus_tag="VBE34_000899"
     CDS             17518..19734
                     /locus_tag="VBE34_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEO7263628.1"
                     /translation="MVKEINLTGEEVVAITSQYMSETDVAFVKFALNYATAAHYYQAR
                     KSGEPYIIHPIQVAGILADLHLDAVTVACGFLHDVVEDTEITLDEIETDFGKDVRDII
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLSFRYLNETEFYKISHMMSEKRREREELV
                     DIIVDKIRSYTEEQGLYGDIYGRPKHIYSIYRKMRDKKKRFDQIYDLIAIRCIMETAS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKEMHQV
                     AEFGVAAHWAYKKGITSKVNQAEQSVGMGWIQELVELQDESKDAKDFVDSVKEDIFTE
                     RIYVFTPNGAVQELPRESGPIDFAYAIHTQVGEKATGAKVNGRMVPLTAKLKTGDVVE
                     IITNPNSFGPSRDWIKIVKTNKARNKIRQFFKNQDKETSINKGRELLVDYFQEQGYVP
                     NKYLDKKHIEEILPRVSVKSEEALYAAVGFGDLSPISIFNKLTEKERREEERAKAKAE
                     ADELINGGEIKTDKRDVLKVKSENGVIIQGASGLLMRIAKCCNPVPGDLIEGYITKGR
                     GVAIHRSDCQNLKSQENYEQRLIDVEWDDDGSKKEYMAEIDIYGLNRSGLLNDVLQTL
                     SNATKLVSTVNAQPTKDMKFANIHVSFGISNLAQLTTVVDKIKIIPDVYSVKRTNG"
     gene            19744..20187
                     /locus_tag="VBE34_000900"
     CDS             19744..20187
                     /locus_tag="VBE34_000900"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEO7263629.1"
                     /translation="MKIIIQRVSQASVSIEDDVVGSIEKGLVLLVGIAPEDTTEDIAY
                     AVRKITSMRIFSDDEGKMNLSIQDIKGSVLSISQFTLFADTKKGNRPAFTGAADPVKA
                     NQFYDIFNQELANHVSVETGQFGADMQVSLINDGPVTIVLDTKNK"
     gene            20442..21365
                     /locus_tag="VBE34_000901"
     CDS             20442..21365
                     /locus_tag="VBE34_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEO7263630.1"
                     /translation="MKRIRKSLIFVLGIVTLICLCACTKQSQQKNGLSVVTSFYPVYS
                     ITKAVSGDLNDIKMIRSQSGIHGFEPSSSDVAAIYDADLFLYHSHTLEAWARRLEPSL
                     HHSKVSVIEASKGMTLDKVHGLEDVEAEKGVDESTLYDPHTWNDPVKVSEEAQLIATQ
                     LAKKDPKNAKVYQKNADQFSDKAMAIAEKYKPKFKAAKSKYFVTSHTAFSYLAKRYGL
                     TQLGIAGVSTEQEPSAKKLAEIQEFVKTYKVKTIFVEEGVSPKLAQAVASATRVKIAS
                     LSPLEAVPKNNKDYLENLETNLKVLVKSLNQ"
     gene            21392..23974
                     /locus_tag="VBE34_000902"
     CDS             21392..23974
                     /locus_tag="VBE34_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO7263631.1"
                     /translation="MNRKKTVIISALSVALFGTGVGAYQLGSYNAQKSDNSVSYVKTD
                     KSDSKAQATAANKTPDQISKEEGISAEQIVVKITDDGYVTSHGDHYHYYNGKVPYDAI
                     ISEELIMKDPSYVFNKADVINEVKDGYIIKVNGKYYLYLKEGSKRTNVRTKEQIQKQR
                     EEWSKGGSKGESGKHSSAKTQALSASVREAKASGRYTTDDGYVFSPTDVIDDMGDAFL
                     VPHGDHFHYIPKADLSPSELSAAQAYWNRKTGRSGNSSKPSNSSSYIHASAPSGNVST
                     GRHANAPISIPRVTHANHWSKPAGNHATAPKHHAPTTKPINKDSALDKMLKRLYAQPL
                     SARHVESDGLVYDPAQVNAFTAIGVSIPHGNHFHFIHYKDMSPLELEATRMVAEHRGH
                     HIDALGKKDSTEKPKHISHEPNKEPHTEEEHHAVTPKDQRKGKPNSQIVYSAQEIEEA
                     KKAGKYTTSDGYIFDAKDIKKDTGTGYVIPHMTHEHWVPKKDLSESELKAAQEFLSGK
                     SEANQDKPKTGKTAQEIYEAIEPKAIVKPEDLLFGIAQATDYKNGTFVIPHKDHYHYV
                     ELKWFDEEKDLLADSDKTYSLEDYLATAKYYMMHPEKRPKVEGWGKDAEIYKEKDSNK
                     ADKPSPAPTDNKSTSNSSDKNLSAAEVFKQAKPEKIVPLDKIAAHMAYAVGFEDDQLI
                     VPHHDHYHNVPMAWFDKGGLWKAPEGYTLQQLFSTIKYYMEHPNELPKEKGWGHDSDH
                     NKGSNKDNKAKNYAPDEEPEDSGKVTHNYGFYDVNKGSDEEEPEKQEDESELDEYELG
                     MAQNAKKYGMDRQSFEKQLIQLSNKYSVSFESFNYINGNQVQVTKKDGSKVLVDIKTL
                     AEVK"
     gene            complement(24255..25031)
                     /locus_tag="VBE34_000903"
     CDS             complement(24255..25031)
                     /locus_tag="VBE34_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO7263632.1"
                     /translation="MLKRERLQKIIEKVNINGIVTVNEIMEELDVSDMTVRRDLDELD
                     KAGLLIRIHGGAQKVNAAPIPQNYEKSNTEKYDIQTNEKLEIAQFAKQFINDGETIFI
                     GPGTTLEKLATQLLDFKIRVVTNSLPVFNILNQSSTLDLILVGGEYREITGAFVGSVT
                     INSIKSLNFSKAFVSSNGVFEKSIATYDEGEGEIQRIALNNSFEKFLLVDSQKFGKYD
                     FYTFYQLDDIDFVLTDHNIDNVVKEQYSSFTKILTNNNYM"
     gene            25240..25713
                     /locus_tag="VBE34_000904"
     CDS             25240..25713
                     /locus_tag="VBE34_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7263633.1"
                     /translation="MFAVTQDILFIDAHSQEELFDLVSKALIKQHYVSPDYRQAVKER
                     EREFPTGLKIDLKDGTPIQYVAIPHTETQYCLVDRIFYVKNSQPITFKHMINPEEECR
                     VQDFFFIINSRNSNQSDILSNLITFFITKGNLDRLHELGDNKEKINHYLIEKGVF"
     gene            25715..26020
                     /locus_tag="VBE34_000905"
     CDS             25715..26020
                     /locus_tag="VBE34_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7263634.1"
                     /translation="MIKILAACGAGINSSHQIKDAIETQLGDRGYNVHCDAVMVKDIT
                     EEMVNKYDIFTPIAKTDLGFNVPIPVVEAGSILYRIPVMSEPVFTALEQVIKEHNLS"
     gene            26060..26462
                     /locus_tag="VBE34_000906"
                     /pseudo
     CDS             26060..26462
                     /locus_tag="VBE34_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195826.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
BASE COUNT         8504 a         4247 c         4998 g         8713 t
ORIGIN      
        1 acctttgaaa aaccagttca attattggaa tcaatctgtg aaaaaggtat gcataattta
       61 tatttcttag acattgaaat aaaaaatgat gagcagaggg gcttagaggt cgcaaaacaa
      121 attagacaaa ttgatcctta tgctcagatt gtttttgtta caactcattc ggaattaatg
      181 cctttaacct ttcgttatca ggtatcagct ttagactata tcgataaagg actttcacag
      241 gaagcattta gcagaaggat tgaagacgtt ttactatatg ctaataatgc ttgcagtaaa
      301 aacttactag aagattcttt ttattttaaa tcacgctata gtcaagtaca agtacctttt
      361 aacgatatcc tttatataga aacgtcatta aggtcacata gagttgtcct ttatactgca
      421 aaagacagaa tggaattcac agccactttg ggggatattt taaaacaaga acctaggctc
      481 tttcaatgtc atcgctcttt tttagtaaat cccttgaata tttttaaagt ggataggtca
      541 aataaactaa tctatttaaa aaatggttcg aattgtttga tttctcgtaa taaaataaaa
      601 gatattatgg tagtaataga ggaaaatcaa agaggtagag gaatatgaat tctactctat
      661 ccatcttaga gatagcttta acgataatat taacaatatt tctcttttct aaaatcagtg
      721 gacgtcaatt gactttaata gagattggaa tttgtattgc tctgagatta ggacttgtaa
      781 ttttatttat agcagctaat actattatta atctttcatt tctaaagtac tggcttctcc
      841 ccatctatat gttaagtctg gctttcttgt tgctaagacc tatatctaaa actttagtag
      901 tcttttatgg cttgtttcca attgttctca gaaatctttg ttcacgttct ttagcttttt
      961 ttgtctttcc tttgtttaat attgattttc atacaaccaa ccaggggttt tggggatatt
     1021 ttgttgatgt cttgtctact ctttgtgttc tcctattttt aagatggatg caatacgact
     1081 ttaaaacttt gcgaagtagt gaagtttcgc ccatagataa gagaattctc caatttacga
     1141 atggttgtat ggtcagttat tacttactga tgcagttttc aacttatttg gaatatgaat
     1201 tagccatcaa tactgttatt taccggcaat ttttgcttgt tgtttattgg attatattta
     1261 cgggagcaat agccagttta gaccggagac ttcgaaattc tttacaggag aggttagctg
     1321 ttcaaagaga actccaattg gctaatatga tagattatag ccaccatatt gaggggttgt
     1381 ataaagaagt taggagcttc cgacatgatt atagtaacat gttaacgacc ttgaaactag
     1441 ggattgataa tgaagatata tctattgtca aatcagttta tcagtctatc tttaaagaat
     1501 ctaatacaca tttccgacat cctaagtata atatagaccg gttggttaat gtagataacc
     1561 cagccttaaa aagtctttta gcagccaagc tttcacaagc gatagacaat catattatgg
     1621 tatctataga agtaccagac agtattaaac ctcaagggat ggaactgatt gattttatta
     1681 ccattgtatc tgttttatgg gataacgcta ttgaggcatc tttagagaca gacgatccta
     1741 agatagccat gtcttatttt acattaaaac ataaacaaat ttttatcata gaaaattcaa
     1801 ctaaggaaaa ggcaatagat acgtcaacga tattcaatta tgagataagt agtaaggggc
     1861 ggaatagagg aataggtctt tacaatgtca ggggagttat caatcgttat ccaaatattt
     1921 ctattgcaac aagtagccaa gattttagtt tctgccaaac tttcgaaatc atattatagt
     1981 tttaaaaagt tcaatttatc taagtttatt attttcttaa taccatcaat ccaaccacca
     2041 ccagtgatat tatttagttg ttccggcgat aattcctgaa gctgtagttc catctcgatt
     2101 agttttggct agttctcgta agtggtttaa taaatatttt gaaccataga actttgcgat
     2161 tgaagcaagt gcagttactc cacaatcctt agtatcaacc tgacgcacca atgctttttt
     2221 gtacgtcaac attgtgtaat tcctttcttc gttatcgtat taatattttg ccataaaaat
     2281 atatacttat taaaaatagc ttaaacagct attgcaacgc gtccaatggt ttctataagg
     2341 atataaaggt agatagtact tttctgtttt gaggcgattc aacgacattt cagggaataa
     2401 acgtttctat ttttatttga atgctatatt aaatgtgtca agttgcaaca gacaaaaatt
     2461 ttatatagga gatattcaaa ataaatgtaa aatcaattga acaattagaa gttatggata
     2521 atgaagcatt atcacatgtt gaaggtggcc ttggtcatag atgggtatac agtaagaacc
     2581 ggtaaacata cttatacaag tgcatggcat ctttctgaaa atactgctgc agcaaatgca
     2641 agaaatcgta tgggccttta ccctggaagc gtttgtcgtg tctatcttgt ataattgatt
     2701 ttttatttaa gcgataggga atcttccttg ccgcttaagt ttgtataggg agagacaata
     2761 ctatgttatt acagctagat aatatcacta aaagctacgg aaaaaaaata gtacttaacc
     2821 agatttccta ccagtttact cctggtttgt atggactctt aggagcaaat ggtactggca
     2881 aaacaacctt attaaatttg atgagccact ttacattcgc agatagtggg aatatttatt
     2941 ggaatggaca ggagcagtcg gaagaatttt atcgtcacat tggtttttta ccgcaacatt
     3001 ttcgctatta tgatcaattt actggcattg ctttcttaaa ttatatcgct accttaaaag
     3061 gtgttgataa aaagaaagct aaaccagaaa ttcctagatt actagagctt gttggtttag
     3121 gtgatgtagg taaaaagaag atttcatcat attccggtgg tatgaagcaa cgtttaggca
     3181 ttgctcaagc acttatcaat gaccctgaaa tacttattct ggatgaacca actgttggac
     3241 ttgatcctaa ggaacgggtt aaatttcgcc atattttgag tcagttatcc accaataaaa
     3301 ttatcatttt atcaacgcat attgtgtcgg atgtggaagc tgttgccaaa gaaattatag
     3361 tattaaaaaa tggtaaattt atcgaacagg gcaacaccgc acagttgttg aaaactattg
     3421 agggaaaagt gtgggaaata acgactgaac cagatttatc tcagataccc aatattgcta
     3481 ttgttaatga aaaagtcatt tcagatagtc tggtatttcg tgtagtgagt gatatttgtc
     3541 cgagtgattc ggcacagttg gttgttccga ctctagaaga tttctacatc tatcatttta
     3601 gaggggagta agtaagatga tatcaatttt tccttttgaa ttaaaaaaac ttctacaacg
     3661 taaggcagca ttgggggctt ttattatctt ccttcttgct atttttggtc tattttacca
     3721 gcattttttt gttgggcaaa ttagtggcta ttctgcagat aaaatacatg gtcgagaagc
     3781 ggttgcaatt aatcgtagaa tagctgaaaa atattcaggg gatttatctg aagaacgagt
     3841 aacaaatatt attaaaaact atgttaatcc gccagcagga cgccccccta tatttgatgt
     3901 ttttagttat tatgtggcag acacttttga tcgtgatttt ttaatttact ataaccaatt
     3961 gaaagatgat aaaacaattg attttaataa aataaatatt aaagatcaaa aacagttggg
     4021 ttctgcgcta ccactaaaac aactaaagct aggtaacttt gctccttgga atcaactttt
     4081 tgaagtaagt aatagtactt atattttaat tgtcttattt actctattct tatcagctcc
     4141 tcttttttca ggagataggg ctaagaagat taatcctata ctactgacga ctcggtatgg
     4201 acgtggtcag ttgacctggg ctaagttagc tagtacaatg accatcggta tttccacatt
     4261 tatcctagca tatgccctca ttctgacggt atttgcagat tattttggtt ttagtggttg
     4321 ggagacaagt gttcaactga atttatactg gacatcacct ttggctaata ttatgggatt
     4381 tcctgtacct atgacaatat caactgcttt tgttcatatt attgtcttac aatttgttgg
     4441 actccttttt atagccagtc ttaatttctt tatattaagt atcacaaatt cgccattaac
     4501 atcttttgct gtttcaacgc ttgccttttt tgctcctagc tttttaatgg ctatgtttaa
     4561 taaaggtgtg ataaacaagt tattaactat tttctcagta tcaacgacag acacttcagc
     4621 tttactttta aaattatcac gtaatggtag tcatggcttt ttctttgata gtttttctgc
     4681 taatgaaatc tgtctgatta gtctacgtat gttaattgca gtgttattga tagcttatac
     4741 ctatcgttta atgcgacgaa gaagtttata gttcccactg gttatctgaa caagaaatta
     4801 ttctaagctg attttctttt gcgaaatcag cttttttatt tcctaatatg gttgataagg
     4861 tattaacaat aattatcaga taccatttaa ggcgtttcaa tgacttttgg ggataattga
     4921 gatattcttc tgaagagatt ggtatcatag taatgataac ttatagaaag taatgttaag
     4981 gatgaagaga agtcagtgca tggataataa gcagcataat acatcaatga ttagcttgct
     5041 acaatatctt ttttccatac tggtcatttt agttcatagt ggaaggttat ttagtcagga
     5101 tgtcattcat tttaccttta aaagtttcct aggtcggatg gctgttcctt attttttgat
     5161 ttgtactgct ttctttttga gaggtcgtat acagcaaggt ttgtgtaatc actcctactt
     5221 tcgtaaactg attaaaaagt atagcatgtg gactatcatt tatttgcctt atggttattt
     5281 cttttttgaa tctctaaata tagcgaagat ttatttgctg ccgggtttta ttgtggcatt
     5341 tttgtattta ggtatgtcac atactctttg gtacatccct gctgttattc taggttggtt
     5401 cattattcaa ggattattga agtatgtagg aactaggggg acatttatca cagttgttgt
     5461 actctattgt attggagcag tagagaccta ttctgtcttt gtacagtcaa caaaatttta
     5521 tcccctgatg tccacttata tgtcaatttt tcagacaaca cgaaacggat tattttacac
     5581 tccagtttat cttctagcag gatatttact ctatgattat tttaatactg acctatttac
     5641 caaatcgcgt ggtcttaagt atattctctt ccttttactt ctagctttag agaatgttct
     5701 tatttatttt aatcaagggt tggataaaaa tttcttctta ttagctcctt tatgtgcagt
     5761 attcttattt aactggagta tacgtacatc actgtttaag ggctatcgtt taagtccttt
     5821 aaaacagttg agtgtctatt atttcttcct tcaccctctt tttgttggca tagtgtctta
     5881 ctgtttaaaa tcaacttcct tagttgcaca ccatcaagga aaagtgattt ttgtggtgac
     5941 cttggcatta acacatgctt gtgctctact cataataaag gttgttaata agatacccta
     6001 tcaaaaaatt attccattat ctctttcgaa atagtgattc atataagaga aagtttaaag
     6061 ttatagtgat gtaaaggttg tgtaaactat taggatacat gaccttttta ttgttctgtt
     6121 tttaaatatt aattatatag aatagtccac acatagagta gaatagtcca tttttaaacc
     6181 gttttcattt gttagaatga tagtaaaggt tatcttattc aaagtattga taaaactttc
     6241 agaactaatt tagggtaatg taataaatag gaggagaaag atgaagcgga aattcctaat
     6301 cggcagtcac ggaaaacttg cgagtgggtt gcagagttct attgatattc tgacaggcaa
     6361 gggacaagag attcagacaa tagatgctta cattgatgat agtgactata ccaagtctat
     6421 tgtagaattt attgatgaga ttgcaccaga tgaacagggg cttattttta cagatttgtt
     6481 aggtggcagc gttaatcaga aaatggcgac tgctgtaatg aatagtggga aaaataatgt
     6541 ttttttaata acgaacagca atttagcgac cttgttgtca ttactatttt tgaaacctga
     6601 agaggaatta actaaagaag aaattgtaac cgttatcaat gaatctcaag tgcagctggt
     6661 agatctcagc tttaaagcgt gttcagaaga tgactttttc gattaataat taggaggaaa
     6721 aaaatgatta ctcaaattcg tgttgacgac cgtttgattc acgggcaagt ggcagttgta
     6781 tggactaaag aattaaatgc accactttta gtggtggcta atgatgaggc tgccaaaaat
     6841 gaaatcacac agatgactct taaaatggct gtgcctaatg gcatgaaatt attaatccgt
     6901 tcagttgaag aatcaatagc tctatttaaa gatccacgcg caactgacaa gcgcattttt
     6961 gtgattgtca attctgttaa agatgcctgt acaattgcta aaaatataac cgatttagaa
     7021 gcagtaaacg tcgctaatgt aggacgcttt gataagtctg atccagctac aaaagtcaaa
     7081 ctaacatcaa gtctcttatt aaatccagaa gagttggaag ccgcaaaaga attagccagc
     7141 ttaccagatt tagacgtctt taaccaagta ttgccttcaa atactaaggt taacttgagt
     7201 cagctagtca aacaaagtga ggtatgacat atgataatgc cagcgacgat ggcagcttta
     7261 gctgtgttga tttgttttgg gggaaattac ttaacaggtc aaagtatgat ggagagacca
     7321 cttgtagttg gtcttgtaac aggaatgcta ctaggtgata ttaaggttgg tatcttaatg
     7381 ggtgcttccc tagaagcgct atttttggga aacgttaata ttgggggtgt tattgccgca
     7441 gagccagtaa cagcaacagc gatggcaaca acttttacca ttatttcaaa cattgatcaa
     7501 aaagcagcaa tgactctagc tgttccgatt ggaatgttgg cagcatttgt tgttatgttt
     7561 ttgaaaaacg tcttcatgaa tatctttgca ccgatggtgg ataaagccgc agccgcaaac
     7621 catcaaggta aacttgtgat gcttcactat ggtacatgga ttatttacta tctcattatc
     7681 gcttctattt ctttcattgg tattttagta ggtagtgggc ctgttaatag ttttgtccat
     7741 catatttctc aaaacctgat gaatggcttg agtgcggcgg gaggcctcct tccagctgtt
     7801 ggttttgcca tgctgatgaa attattatgg acaaataaat tagctgtttt ttacttgctt
     7861 ggttttgttt taacagctta tttaaaatta ccagcggttg cagttgctgc cctaggagca
     7921 gttatttgcg tcattagttc acaacgtgat atagaattag acgcaattac tagaggtgcg
     7981 atcagtaaac agacaacctt tgactctaaa gaatcagaag aggaggactt cttcgcatga
     8041 aatcacagga taatttgact aaagaagacc gtaaaatgct tcgctcagtc ttctggcgct
     8101 catggacgat gaatgccagt cgtactgggg caacacaata ccacgcagta ggagttattt
     8161 atactttact accagtcatc aatcgttttt ataagactga taaggataaa gcagaggcac
     8221 tggtccgtca tacaacatgg tttaatgcca ccatgcacat taataatttt attatgggtc
     8281 ttgttgcatc tatggaaaag aaaaacagtg aagatcctga ttttgacgct agtgctatca
     8341 ccgcagttaa ggcttctttg atgggaccta tatctggtgt tggtgattca ttcttttggg
     8401 gaattctaag ggttattgcg gcaggtatcg gtatctcctt agcaagtaca ggctcagcca
     8461 tgggagctgt tgtctttcta ctcttgtata acattccagc ttttctgatt cattattata
     8521 gtctctatgg aggttactct gtaggtgcag ggtttatcaa aaaattgtat gaatcaggtg
     8581 gtattaaaat tgttactaag acttcaagta tgcttggttt aatgatggta ggctcaatga
     8641 ctgcttcaaa tgttaaattc aagacaatct taacggtagc agctaaagga gctaaggaag
     8701 cagcttccat acaaagttac cttgaccaat tatttgttgg cgtagtgcca ctgttggtaa
     8761 cgatattagc cttctggctt ctacgtaaaa aagtcaatat caactggatc atgtttggta
     8821 ttatggtgtt aggtattgta cttggtctct taggtatttg ctaataatct cctagcatta
     8881 aaggtaagtg gaacaagacg atacttgttt agcttatctt tttttctttt gaaagttatt
     8941 gtatgaaagg agttgactat ctttattgga tagctatttg ctataataaa aggagatgga
     9001 aatgagagga tatagaatgg aagaacgctt taagaaacga ctacaagatg atatctctaa
     9061 acatttttcc cgccagtctt taattttatc cttgttattg attgcacttt tcgtcctttt
     9121 ttctttagcg cctcagcaga taggccttta taaagatgtt aattcagttt cctatagtta
     9181 taagcaattg attcaaaaac atgatacctt attagatgac ttaggtaaaa atagtttgaa
     9241 accatttgtc tcaggacact taggaagtgc tgatttgagt aagcaatatt atcatttaag
     9301 gaatcattta caaagtcaaa cagaactttt agtattctca cctaatcaag aacttttgtt
     9361 tgctagtaat tcacatttag gtaatttttt tagtaagtct atttacatta gtgaagtttt
     9421 agataaggct aaaataaatc aaaggctact aaaaattatt gtagatagtg agggagggca
     9481 ttaccttgca ctcattaagc caattatagt taataaaaaa gtttcaggtt atgccttttt
     9541 attgatgaat gggaaagact ttttacttcc aacgaaggct attaactcag acttgattat
     9601 tgccgatcag ctaaataata gctttacttt tactaatcgt gattttatct cctctagttt
     9661 agataaggta gatagtcaat tcttaactag atattttagt tttcacgatc atcgagcttt
     9721 tgttgttagg aaagtagctc tgcaagataa tatcctactt tatatgtatc gtcccctgat
     9781 accagtaact ctggtcgtcc ttttttctct agtatcatca gttattattt ttgtgatttt
     9841 acgacgaaaa tctagagttc tagccgatcg catcgcagta aaaaattctg gcgccataaa
     9901 tcaaatggtg ctagatatgg atgccatttc ccgccaagaa aaatctagta ttgagcttga
     9961 tagtcaagat gaatttcaat atctatctgt tcaaatcaat cagatggttt cacggttaaa
    10021 agatttacat gagaaaactc tcgatttgga aacacaaaag ttgctgtttg aaaagaggat
    10081 gttagaagct cagtttaatc ctcacttcct ttataatacc cttgagacaa ttttaataac
    10141 cagtcactat gattcacaat taacagaaag aatcgttata caactaacaa aattattgcg
    10201 ctatagcctt agtggtagca cagaggcagc tgtgcttaag gatgatttag caatcattga
    10261 atcctacctt ttgattaatc aagtgagatt tgaagaatta acttacacta tttcagtatc
    10321 tcctgaactt gaacacatgc gtgtaccaaa acttttcttg ttaccgctaa ttgagaatgc
    10381 tattaaatat ggcttaaaag aacgtcatga tgtagcaata aacatagata tttggcaaga
    10441 tagtgatgga atttggttta ctgtttcaaa taatggatcg ggtattagtt tggctaggca
    10501 aaaagctata cgtacaatgt taagatcaaa tcactcgcat catgggctta ttaattctta
    10561 tagacgatta caatatcaat tttcaacagt attgttagag tttacgaaga cagatgatgc
    10621 ttttcgagtt agctatatag taaaggagtg agttatgtat agattactta tcgttgagga
    10681 tgagcacctt attcgtaagt ggttgcgcta tgctattgat tatcaatcat tgaatatttt
    10741 agtggttggt gaagcaaaag atggtaaaga aggagctcaa ttaattcagg aagagcagcc
    10801 agacattgtt ttaagtgata ttaatatgcc aattatgaca gcattcgata tgtttgaggc
    10861 aaccaaagga caatcttatg ccaaaattat tttatctggc tatgctgatt ttccaaatgc
    10921 gcaatcagct attcactatg gcgttcttga atttttaaca aaaccactag aaaagcaagc
    10981 tttgattgat tgtttaaaaa caattatggc tagaattgag gaacataagg agaagcacct
    11041 acaagagcat acagaattgt atttacctct accacaagct aatgaccaag ttcctgaagt
    11101 tattaaagat atgctagcat ggattcatag ccattttcat gggaaaattg tgattagcca
    11161 gttagctcac gatttaggtt acagtgaaag ttatctttac acagtcacta aaaagcacct
    11221 tcatataact ttgagtgatt atataaatca atatcgtatt aatcaagcca ttcagttgat
    11281 gtttagagaa ccagacctaa tggtttacca aattgcagaa gctgttggta tttatgatta
    11341 tcgttatttt gatagggttt ttaaaaagta cttgggtcag acagtaaaag cgtttaagga
    11401 agaacatatt tttaaacaga tggattaggt aggtgaaaga tgaaagaaaa acagtcgaaa
    11461 aggcttattt atatactact gattgttccc attatcttta taagtgtttt tacatacagt
    11521 attagccagc cttctaaact acttccacca aaagaattag ttattctaag tccaaatagt
    11581 caagccattt taacaggaac gattccagct tttgaggaaa aatacggtat aaaagttaag
    11641 cttattcaag gtgggacagg gcaactaata gatagattaa gtaaggaggg taagcagttg
    11701 aaggcggata ttttctttgg aggaaattat acgcaatttg aaagtcataa ggcattgttt
    11761 gagtcttacg tatcaaagaa tgttcatact gttattccag actatatcca tccgagtgat
    11821 acggcgacac cttatactat aaatgggagt gtcttgattg taaataacga attagctaag
    11881 ggacttacca tcaagagtta tgaagattta ttacagcctt ccttaaaagg taaaattgcc
    11941 tttgcagatc cgaatacttc ctctagtgct ttctcacaac tcactaatat actcttggcc
    12001 aagggtggtt acaccaatcc aaaagcgtgg aactatgtta aaaagctaca acataatatt
    12061 aatgctatca aatcttctag ctcttcagaa gtttatcaat cagttgcaga aggaaaaatg
    12121 attgtggggt tgacttacga agaccctagt gtcaatttgc aaaaaagtgg tgccaatgtt
    12181 tctattgtat acccgacaga agggacagtt tttgtcccat cttcggttgc aattataaag
    12241 aatgctcctt ctatgaaaga agcaaagtta tttattaatt ttatgctttc tttagatgtt
    12301 caaaatgcct ttgggcagtc aacgagtaac cgacctattc gtaaagatgc ccaaacaagt
    12361 aatggcatga aagctttaaa ggatatcgct actcttaaag aagattatcg ctatgtcact
    12421 aagcataaga gccaaatcct taaaacctat aatcgcattc gtagaaatgc tgattaaatg
    12481 aaaatataga aaaactcctc ggaatcattg attccgagga gtttaatcat cgtcctcgtc
    12541 gctttcgaaa tcttttttcc aataacgata aagactaatg catgccagaa atccggctat
    12601 aataggaacg ataataaata agaggtcaat cactgtatcc atgggcaata tactccaatg
    12661 ataagaagtc attattgttt atagtgcgta caataatgtt aaggtaaaac gtcggtcatt
    12721 ataagttttt tgacatcctc taaactcata gattttgtta atattttttc aggtatctct
    12781 tttaagaagt ctttctcaag ccaccacctt gcaagagaat cttctccaaa ttctaaagat
    12841 ttaagtcgag ttgaatgtct tttaacagtt tcttgaaagc ttaaatcata gtaataagca
    12901 taagtattgt tggggttatg ttttaaaata tgtttccaaa caggcgtata ccaatcagaa
    12961 cgaagtatgc cttcaaggat gatgtaggaa cagttatggt aaccataatt tagtaaattg
    13021 atcaaaaggg gaatggtagt agtattttca ccatccttag ttcctaacat ctctctacga
    13081 aggtaatcct gagataagag aagggtatct tcccctaatt cggcttgaag ttgtttagct
    13141 attgtagact ttccgctagc tgaatttccc ctgataataa tgagcgtggc taccattagt
    13201 tcaccatatt gcttttgaga taggcatcca ctaagagttc agttgcttga atagaatcga
    13261 tatgggtacg ttcataagaa tgactagatt caatacctgc accaagtagc gcgtgtttaa
    13321 cttccgcacc agcacgcatg gcagcggagg cgtcactacc ataataagga taaatatcaa
    13381 gtttataagg aatattattg ttttcagcta gagaaacaag atgttgacgt aattcataat
    13441 gataaggacc agaagcatct ttaacacaga tagagacagt atattcgtca gtttcttgat
    13501 cgtctcccat agctcccata tcaactgcta gatattctac agtttcattt gggatgcttg
    13561 aatttgctcc atgtcctagc tcttcaaagg cactaaagta gaaatgagta gtataaggaa
    13621 gttgaatgtc ttctttcttg taaacagaaa gaagttccat taggatacca gcggatacct
    13681 tgtcatctag gtaacgtgat ttaataaagc cgctgcctgt tatgattgta cgcggatcaa
    13741 atgaaatgaa atcaccgacc tggatgccta aagcacgtgt ttcgtcagca gttgttactt
    13801 tctcatctaa acgaatttcc atatttgttt gattacgttc agcagttcca gcatctttgt
    13861 aaacatggac actagtttga tgaataagag cagttccaga aatttcttta ccattttttg
    13921 agaggtgtat agtacagttt tctccttcaa tggcattgta tgtgtatccg ccaataaggt
    13981 caatttttaa ccgaccatct ggtttgatgg ccctaaccat agcaccaagt gtatcaaggt
    14041 gagcagttat catgcgatgt ttagtattat tttttccttt tagagaaacc atgacgcctc
    14101 ccttgtttgt gcgaattggt gagtagccaa acgcttccaa ttctttgatg atataggtca
    14161 tgattgtttg ggtaaaccct gttggtgaag ggattgatgt cagggtttta atgtagttta
    14221 atgttgtttc catactattt cctttaggct ttaaagttcg ataatgtttc aacgataata
    14281 tttgcgatac gttcaacgtc gctagaagtt cctcgcaatt caaagtctcc taacatagga
    14341 aatccaaacc gttcagagta ttgttttgca gttaaacagt actgattgtt aaaatttcta
    14401 tttccagagc caacaattcc gatacaattt ttgacattat tgtgtgcagc tataaaatca
    14461 ccaagtggaa tggtaagaat ttcttgatcg ccattgtcaa tgccatttcc tccctcgagg
    14521 taagtgggta atagtgctac gaaaggttgg tcaacaggaa aggtttgatg attttgttcc
    14581 ttgatattaa tagtcgaaac ctgacagtta tgccaagttg acagttgttc actaagacgt
    14641 ttgacaaagc tttgagtatt gccgctaaga ctgatataga ctagggttaa tttaggcatt
    14701 tagtactcct taaatctaaa tttcttgtta tcagtttagc aaaatcatgt cagataaaca
    14761 agctagagtg gtatctcaat atctgactgt ttagagagaa aatctattgt tgagactaaa
    14821 aactgaggtt gattggcctc agtttttagt gatgactata tttgtttctt tttaagtaaa
    14881 tttcccacga gtccaagtat tgcgacgcca aagaaagcca gataagattg tttttcttgt
    14941 ccagttttag gtaatgtttt ttctttctgt tgtgtataga tagtgcctgc gatatttgga
    15001 gaagcttttg cttttgtata agcagttgca accatgtggt gaattttttg tttctctatt
    15061 tggcttaact tttcttgatg tgatggctca gtaaggtcta ctttttcttg ttcagtatag
    15121 acaaatcgat aatcaccaaa tccttcatct ttggtagaac tagcgatata ctgaatatcc
    15181 ttgctattag taacaagatt tttagcttta tcggctgttt ggaatcgtaa atcaaggcct
    15241 ttaatagact ctgtgaagcc ccagttatta tcagctgtag gattgatggt tttctctgaa
    15301 ataatatagt ttataatagt ttggcgattt tctaagttca gcaaacggtt cattgtggca
    15361 tttttagccc cagggaaggt accactagca cggtagttat tggtcacaat cataaactct
    15421 tgtttgtctg caacaggctt accattgtac attaaatcgc gtactcgact agcttgacta
    15481 ttaacaactt ttccatcttt gttgtattta ttaggttgtg taatatcaaa tttataagtc
    15541 aagccatcaa taacgtcaaa attataggta cgatattcgg tattgataat attttgcggt
    15601 gctttattgt tagggtcaat ttggttaaat tgaccagcgg acatttctaa ccattcacgt
    15661 aagtctgctc cggtcacttt taaaagtgca gtaacattat cataaaggta aagatcagct
    15721 acgtttttaa ttgcaacagg tccagctgga atatcagtat atgcggtagc atcgccacgt
    15781 gttccggctt taaatggtgc tgcagcagat agaagtggta aatttgcctc aggtgttcct
    15841 tttaattcat ttgcaacgta ccaacgttga gcgttgttaa caatttgaac tgaagggtca
    15901 tctttaacta gtgcaaagta acttgtaatt ggcgcagttg ttgtacctac ttgttggcga
    15961 acatagttga tagtgccatc gtgtgcttcc ttagccaatg caatgatacg ctcgtcagca
    16021 gttgttgagt tcatatcaat tttacggatt ttagcactgc tttcggagac ttgccatttt
    16081 ccattagtat aactaagtcc taaatcaata ataccaaggt gatccccgta cttgcctgcc
    16141 attgtaacag gtgttccatt tatttttcca ttgataccat caactccagt gtatttttca
    16201 tagaagccag taccgttacc tgatggaaat tcagcgtgtg agtgtcccgt aacaacggca
    16261 tccactccct tgatgctggc aatttgatag ccaacgtttt cttcaccttc ttcatatcga
    16321 tcatctccaa tgccagagtg tgataaaacg agaatgacat ctgctccttt agcacgcata
    16381 gtaggaacga tttcttcgat tgctttaact gaatctttga caataacctt tccctctaag
    16441 ttagccttgt cccagtttaa gatttgagga ggtacaattc ctgtaattcc aatatttagt
    16501 gcaacagctc ttcctgttga atctttgaag gttttagtaa tgatgtcata agttttaaaa
    16561 actggttgat gtgttttgaa atccaaaaca tttgcgttga ggattggaag gttggctgta
    16621 gcaatgattt ttttgaggta atctaaaccg taattaaatt catgattacc tagtgttgat
    16681 gcatcataac caagagcatt catggcttgg tacataggat gttcttcatt ttctgcgaca
    16741 ggtttaacga ttgctttata agttcctaaa ggtgtccctt gaatgacatc tccgttatca
    16801 actaagacgg tattaggatt ttcttttttt gcttcttcga taaggacagc agtttttgct
    16861 aaccctattt tttgagattc tttatcttga taataatcat agttaactaa attggtgtgt
    16921 aggtctgtgg ttgacatgat gcgaaggtca actttttcac cttcaatagg tttgagatca
    16981 ccagttgtgg tttctgtggc aacaggctgt ggattttgat tattggcagt ggtatttgtg
    17041 cttgtggctg ttgaagcatc agttattgca gttggagtag aagtaacgtt tgacttttcc
    17101 tctgcaattg tgctagtggg ggtagttgat tcaacctttg tagcggttga acttggtgtt
    17161 gtaacaatgt cttctgcttg aacagctaaa ccacttgttg ttgtagctgt taagacagtt
    17221 aaagcgaata tgctttttga aaaataatgt tttgacataa atctcctcca ttaaaacatt
    17281 acatgtatat tatcctaatc taaaaactat tatagcttat tgaaaacgtt taatcaatct
    17341 catttacgaa ttatttttag taattacact aaaaacaatc gtttatttat aaaggataac
    17401 ttggttagac acatttactt ggtatttaat tctttttgag cctctaactt cttttatttt
    17461 taggttaaaa agtgataaaa taggtacaat ctatcactat gattaaagag gataactatg
    17521 gtcaaagaaa ttaatttaac aggagaagag gttgttgcaa tcacatccca gtacatgagt
    17581 gagacggatg ttgcttttgt aaaatttgcc ttaaattacg caaccgcagc tcattattat
    17641 caagcaagaa agtcgggcga gccctacatt attcatccaa ttcaagtagc aggtattctt
    17701 gcagatttac accttgatgc cgttacggta gcttgtggtt ttttacatga tgttgtagaa
    17761 gatacggaaa ttacccttga tgagattgaa actgattttg gcaaggatgt tcgcgatatt
    17821 attgacggtg taacaaaatt gggtaaagtg gagtacaaat ctcatgaaga acagttagca
    17881 gaaaatcacc gtaaaatgtt aatggctatg tctaaggata tccgtgttat tttggttaag
    17941 ctcgctgatc gcttacataa tatgagaacc cttaaacacc tcagaaaaga taaacaagag
    18001 cgtatttcac gtgagactat ggaaatatac gctcctttag cgcatcgttt ggggattagt
    18061 cgtatcaagt gggaattaga agatttatct tttcgttact tgaatgagac ggaattttat
    18121 aagatttctc atatgatgag tgaaaaacgt cgtgaacgcg aagagttggt tgatataatt
    18181 gtcgataaaa tcagatccta tactgaggaa cagggtttat atggtgatat ttatggtaga
    18241 ccaaaacaca tttattctat ttacagaaaa atgcgtgata aaaagaaacg ctttgatcag
    18301 atttatgatt taattgcgat acgctgtatt atggaaactg ctagtgatgt ttatgccatg
    18361 gtggggtata tccatgagtt atggcgtccg atgccaggaa ggtttaaaga ttatattgcg
    18421 gcaccaaagg ccaatggtta tcaatctatt cacacgacgg tttatggacc taaagggcca
    18481 attgaaattc agattcgaac taaagaaatg catcaagttg ccgagtttgg tgttgcagcc
    18541 cattgggcct ataaaaaagg aatcaccagt aaggttaatc aagcagagca atcggttggt
    18601 atgggatgga ttcaagaatt agttgagctt caagatgaat caaaagacgc caaggatttt
    18661 gttgactctg ttaaggaaga tatctttaca gaacgcatct atgtttttac cccaaatggt
    18721 gctgttcagg aattgccaag ggaatctgga ccaattgatt ttgcttatgc tattcacacg
    18781 caagttggag aaaaagctac tggcgctaaa gtaaatggac gtatggttcc attgactgct
    18841 aagttaaaaa caggagatgt tgttgagatt atcacaaacc ctaattcatt tggaccaagt
    18901 cgtgactgga ttaagattgt taagaccaat aaagctcgca ataaaattcg tcagttcttc
    18961 aaaaaccaag ataaagaaac ttctattaat aaaggtagag aattactggt tgattatttc
    19021 caagaacaag gttatgtgcc taacaaatat ttagataaga aacatattga agaaatcctt
    19081 ccacgtgtca gtgttaaaag tgaggaagct ttgtatgcag ccgtaggttt tggtgatttg
    19141 agtccgatta gtatttttaa taaactgacg gaaaaagaac gccgtgagga ggaacgtgct
    19201 aaggctaaag cggaagcaga cgaacttatt aacggtggcg aaatcaaaac cgataagcgt
    19261 gatgttctca aagttaagag tgaaaatggt gttatcattc aaggagcttc aggtttatta
    19321 atgcgtattg caaaatgttg taatcccgtt cctggagatc tcattgaagg ctatataact
    19381 aaaggtagag gcgtggctat ccatcgttca gattgtcaaa atttaaagag tcaggagaac
    19441 tacgaacaac gtttgattga tgttgagtgg gatgatgatg gatctaaaaa agagtacatg
    19501 gctgaaatcg atatttatgg tttgaatcgt agtggcctcc ttaatgatgt tcttcaaacc
    19561 ttatcaaatg caacaaagct agtatcgaca gtaaatgcac aaccaacaaa agatatgaaa
    19621 tttgctaata ttcatgttag ctttggaatt tcaaatttag cacaattgac aactgtggtt
    19681 gataaaatca aaattatccc agatgtttat tctgttaaac gtacgaatgg ttaggagagc
    19741 aaaatgaaaa tcattattca acgtgttagt caagcaagtg tttctattga agatgatgta
    19801 gtcggatcta ttgagaaggg tttagtactt ctagttggga ttgctccaga agatacaaca
    19861 gaagatattg cttatgcagt tcgtaaaatt actagcatgc gtattttttc tgatgatgag
    19921 gggaaaatga acctttcaat tcaagacatt aaaggaagcg tcttatctat ctcacaattt
    19981 acactctttg cagataccaa aaaaggaaat cgtccagctt ttactggggc agctgatcct
    20041 gttaaagcga atcagtttta tgatatcttt aatcaagaat tagcaaatca tgtttcggta
    20101 gaaacaggtc aatttggagc tgatatgcaa gtcagtctga ttaatgatgg accagtaact
    20161 atcgttttag atactaaaaa taaataaaaa atcaaagagt tgggcaaaat gcttggctct
    20221 ttttattgtg gttctaatta tactccttaa cccattcatg tttataaaaa gattcactgt
    20281 agcttggaaa ggcatgaata aaggttgttg atatgaaatt aagtgcactt aacaatatta
    20341 aaaatagaaa aatattagat ttatggtttt tcgttgacac taaaataaga atactctaaa
    20401 acgttaactg gttaagtact ttttatttta aaggaggttt aatgaaaagg atacggaaaa
    20461 gccttatttt tgttctcgga atagttaccc taatttgctt atgtgcttgt actaaacaga
    20521 gccagcaaaa aaatggcttg tcggtcgtga ctagctttta tccagtatat tccattacaa
    20581 aagcagtttc tggtgatttg aatgatatta aaatgattcg atcacagtca ggcattcatg
    20641 gttttgaacc ctcatcaagt gatgttgctg ccatttatga tgctgatcta tttctttatc
    20701 attcgcacac actagaagct tgggcgagac gtttggaacc tagtttgcat cactctaaag
    20761 tatctgtaat tgaagcttca aaaggtatga ctttggataa agttcatggc ttagaagatg
    20821 tagaggcaga aaaaggagta gatgagtcaa ccttgtatga ccctcacact tggaatgacc
    20881 ctgtaaaagt atctgaggaa gcacaactca tcgctacaca actagctaaa aaggatccta
    20941 aaaacgctaa ggtttatcaa aaaaatgctg atcaatttag tgacaaggca atggctattg
    21001 cagagaagta taagccaaaa tttaaagctg caaagtctaa atactttgtg acttcacata
    21061 cagcattctc atacttagct aagcgatacg gattgactca gttaggtatt gcaggtgtct
    21121 caaccgagca agaacctagt gctaaaaaat tagccgaaat tcaggagttt gtgaaaacat
    21181 ataaggttaa gactattttt gttgaagaag gagtctcacc taaattagct caagcagtag
    21241 cttcagctac tcgagttaaa attgcaagtt taagtccttt agaagcagtt cccaaaaaca
    21301 ataaagatta cttagaaaat ttggaaacta atcttaaggt acttgtcaaa tcgttaaatc
    21361 aataggtagt tatataaaga aaggacgcag gatgaatcgt aaaaaaacag ttattattag
    21421 tgctttatca gtagctctat ttggtactgg tgtaggtgct taccaattag gtagctataa
    21481 tgcccaaaaa tcagacaata gtgtttctta tgtgaaaaca gataaatctg atagcaaagc
    21541 acaagcaaca gctgcaaata aaacgcccga ccaaatcagt aaagaagaag gcatatctgc
    21601 agaacaaatc gttgttaaaa ttactgatga tggttatgta acttctcatg gagaccatta
    21661 ccattattac aatgggaaag tgccatatga tgccatcatt agtgaagaat tgattatgaa
    21721 agaccctagc tatgtcttta ataaagctga tgtcattaat gaagtcaaag atggttatat
    21781 tatcaaagtt aatggtaagt attacctcta tcttaaagaa ggtagcaaac gaacaaatgt
    21841 tcgtacaaag gaacaaatcc aaaagcaacg cgaagaatgg tctaagggtg gttctaaggg
    21901 agaatcagga aagcatagta gtgcaaaaac tcaagcttta tcagccagcg tacgtgaagc
    21961 gaaagctagc ggtcgctata ctacagatga tggttatgtt tttagtccaa cagatgttat
    22021 tgatgacatg ggagatgcct ttttagtacc acatggcgat catttccatt atatcccgaa
    22081 agctgattta tctccatcag agttatcagc cgcacaggca tattggaacc gcaagactgg
    22141 ccgatcaggt aatagctcaa aaccatcaaa ttcaagttca tacattcatg caagtgcacc
    22201 aagcggtaat gtatcaacag gaagacatgc taatgcccct atttctattc caagagtcac
    22261 tcacgcaaat cactggtcaa aaccagctgg taatcatgct acggcaccaa aacaccatgc
    22321 acctacaacc aaaccaatca acaaagattc tgctttagac aaaatgttga aacgtttata
    22381 tgctcaaccg ttatctgctc gtcacgtaga aagtgatggt ttggtttatg acccagcaca
    22441 agttaatgct ttcacagcaa tcggtgtttc tattccgcac ggtaatcatt tccactttat
    22501 tcactataag gatatgtctc cattagagtt agaagcaaca aggatggtgg cagagcatag
    22561 aggacatcat attgatgcat tagggaaaaa agattctaca gagaaaccaa agcatatttc
    22621 tcatgaacct aataaggaac ctcacacaga ggaagaacac catgcagtaa caccgaaaga
    22681 ccaacgtaaa ggcaaaccaa atagccagat tgtctacagt gctcaagaaa ttgaagaggc
    22741 aaaaaaagct ggtaaataca caacatctga tggttacatt tttgatgcta aagatattaa
    22801 aaaagataca ggtacaggtt atgtcattcc acatatgaca catgagcatt gggtaccaaa
    22861 gaaagattta tcagagtcgg aattaaaagc agctcaagaa tttctttcag gaaaatctga
    22921 agcaaatcaa gacaaaccaa aaacaggtaa aacagctcaa gaaatctatg aggcaattga
    22981 accaaaagca attgttaaac ctgaagattt attatttgga attgcacaag cgacagacta
    23041 taagaatggt acatttgtaa ttcctcataa agatcattac cattatgtgg aattaaaatg
    23101 gtttgatgaa gaaaaagatc ttttagctga ttcagataag acatattctt tagaagacta
    23161 tttagctacg gctaaatatt acatgatgca cccagaaaaa cgtcctaaag ttgaaggatg
    23221 gggtaaagat gctgaaattt ataaggaaaa ggactctaat aaagcagata aaccaagtcc
    23281 tgcaccaact gataataaat caacatcaaa ttctagtgac aaaaacttaa gtgctgcaga
    23341 agtattcaaa caagcaaaac cagaaaaaat tgtaccgctt gataaaattg ctgctcacat
    23401 ggcatatgca gttggatttg aagatgatca attgattgtt cctcatcatg atcattatca
    23461 taatgttcct atggcatggt ttgacaaggg tggtttatgg aaagcaccag aaggctatac
    23521 attacaacaa ctcttctcaa caattaaata ctacatggaa catcctaatg aattaccaaa
    23581 agaaaagggt tggggacacg acagtgatca taacaaaggc tcaaataaag acaataaagc
    23641 caaaaattat gctccagatg aagaacctga agattcaggg aaagtaactc acaactatgg
    23701 tttttatgat gttaataaag gttcagacga agaagaacca gaaaaacaag aagatgaatc
    23761 agagctagat gaatatgaac taggaatggc acaaaacgct aagaaatatg gtatggatag
    23821 acaatctttt gaaaagcaac tcatccaatt atcaaataaa tatagtgtaa gttttgaaag
    23881 ctttaattat attaatggta atcaagttca agttactaaa aaagatggct ctaaagtgct
    23941 tgttgatatt aaaacactag cagaagtgaa ataataaaag agttgaataa acttagctga
    24001 caactaaaaa caaacaagtc accgcacttg tttgtttttt agttatgatt tctagcgtgc
    24061 gaagatttta atgatatatc taattcttct tttgaacaac cattgtttta cgcttaaaag
    24121 agagttaatg tatttttgtt tgaggaagtt ttcttgtttt gacttttata aaacgactaa
    24181 agttataatc actaaataaa gagccactac taaaagctgt ataacaggtt tctagtagtg
    24241 gctcacttgt tttattacat gtaattgtta ttagttaaaa ttttggtgaa gctactgtat
    24301 tgctccttaa caacattatc aatattatga tctgttaata caaaatcaat atcgtctaat
    24361 tgataaaaag tataaaaatc atatttacca aatttttggc tatcaactaa aaggaatttt
    24421 tcaaaggaat tattaagagc aatgcgctgg atttcacctt ctccctcgtc ataagtagcg
    24481 atagactttt caaaaacacc attactacta acaaaagctt ttgaaaaatt aagtgattta
    24541 atagagttaa tagtaacaga accgacaaaa gcaccagtaa tttctctgta ctctcctcct
    24601 acaaggataa ggtcaagtgt tgaactttga ttcagaatat tgaaaacagg aaggctattg
    24661 gttactaccc taattttaaa atcaagtaat tgcgtagcta atttctctaa ggttgttcca
    24721 ggcccaataa agattgtttc accatcattg atgaattgtt ttgcaaattg ggcaatttct
    24781 aatttttcat ttgtttgaat atcgtatttt tcagtgtttg atttttcata attttgtgga
    24841 attggtgctg cgtttacttt ttgtgcacca ccatggatgc gtattaacag tcctgcttta
    24901 tctaattcat ccaagtcacg cctaacagtc atgtctgata catcaagttc ttccatgatt
    24961 tcattaacag tgacaatacc atttatattg actttttcta ttattttttg taatctttct
    25021 cgctttaaca taataatctc tcctacctct tcattgtata tatcatacca taaatagagc
    25081 taaaataata ataattttga aaaaacaaac aataataaac aaaaataaat aaaaaacgtc
    25141 ttgtgatgtt ttattttgtt ttgtataata ttgatgtaga taagttgata aggcttgttt
    25201 gccttacaac aaaatgtaac cgcttaacta aaaagaaagg tgtttgctgt gactcaagat
    25261 attttattta ttgatgccca ttctcaggaa gaattatttg acttagtatc aaaagcattg
    25321 ataaaacaac actatgttag tcctgactac cgacaggcag taaaagaaag ggagagagag
    25381 tttccaacgg ggttaaaaat tgatttaaaa gatgggactc ctattcaata tgttgctatt
    25441 ccacacactg aaacgcagta ttgtctagta gatagaatct tttatgtcaa aaatagtcag
    25501 ccaattactt tcaaacatat gattaatcca gaagaggagt gtagggttca ggattttttc
    25561 ttcatcatta atagtaggaa tagtaaccaa agtgatattt tatctaattt gattaccttc
    25621 tttattacaa aaggaaacct agatcggcta catgagcttg gcgacaataa ggaaaaaatt
    25681 aatcattatt taatagaaaa aggagtgttt taacatgatc aaaatattag cagcttgtgg
    25741 tgcaggcata aattcaagtc atcaaattaa agatgctatc gagacacaat taggagatag
    25801 aggttacaat gtacattgtg atgctgttat ggttaaggat atcactgagg aaatggtcaa
    25861 caaatatgat atctttactc ctattgctaa aactgattta ggttttaatg taccaattcc
    25921 agttgtagaa gcaggatcta ttctttatcg cataccagtt atgagcgaac cggtctttac
    25981 tgctttagaa caagttatca aagaacacaa cctatcataa catatttaaa attggaatta
    26041 gtcttaaagg agaaggaaca tgcaggtatt tttaaatatt gtcaataaat tctttgatcc
    26101 agttattcat atgggttcgg gagttgtgat gctaattgtc atgacaggtt tagccatgat
    26161 atttggagtg aagttttcta aagcacttga aggtggtatt aagttagcta ttgctcttac
    26221 gggtattggt gctattattg gtattttaac tggtgctttt tccgaatcac ttcaagcttt
    26281 tgttaaaaat acaggaatca atctaagcat tattgacgtt ggttgggctc cattagcaac
    26341 tattacatgg ggatcaccat atacgcttta cttcttatta atcatgctta ttgtcaatat
    26401 tgttatgatt gttatgaaaa aaaaacggat accttagatg ttgatatttt tgatatttgg
    26461 ca
//
LOCUS       DATRUK010000022        26376 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.022, whole genome shotgun sequence.
ACCESSION   DATRUK010000022 DATRUK010000000
VERSION     DATRUK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26376
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.022"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..408
                     /locus_tag="VBE34_000907"
     CDS             <1..408
                     /locus_tag="VBE34_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO7263635.1"
                     /translation="TCAVVSKFNNRAFTKEVFQTICKTLISQNHKLTAEDLFWFNHVL
                     LNCVFVGLCLNSEECLAEMLEVSRQTMVSTHDFHKMPLYFMYQWKYFITIDNDIKSAE
                     NAYQQSIMFSKMIDDKHLIKKLELEWQEDITGH"
     gene            705..1703
                     /gene="ruvB"
                     /locus_tag="VBE34_000908"
     CDS             705..1703
                     /gene="ruvB"
                     /locus_tag="VBE34_000908"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001902090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEO7263636.1"
                     /translation="MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEA
                     AKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAIEKSGDLVAILNDLE
                     PGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPR
                     IANRLLKRVRDYAQIMGDGLIDDNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGG
                     PVGLGTLSVNITEERDTVEDMYEPYLIQKGFIMRTRTGRVATDKAYEHLGYQRFDK"
     gene            1855..2292
                     /locus_tag="VBE34_000909"
     CDS             1855..2292
                     /locus_tag="VBE34_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEO7263637.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMANLDKEDSLSVESRATSTWEHG
                     NPIHRGTQAILQRYQIPYTNDKGSQPISKDDLAYFDYIIGMDEQNVKDLKALSQGTYD
                     DKISLFCDGGVPDPWYTGDFEETYRLVSDGCKQWLTLINNSRD"
     gene            2299..2679
                     /locus_tag="VBE34_000910"
     CDS             2299..2679
                     /locus_tag="VBE34_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263638.1"
                     /translation="MKNFKITRTHLEILSLIIIVVFGLSVFTLTTSSQGVLSYDGGKI
                     KYVGSIVNHHMTGKGKLTYENGDYYKGDFVNGVFEGKGTFVSVHGWSYTGDFKKGQPD
                     GQGRLNAKNKKVYKGTFKQGIYQK"
     gene            2676..4454
                     /locus_tag="VBE34_000911"
     CDS             2676..4454
                     /locus_tag="VBE34_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO7263639.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFKNSFPGGFVGVDIFFTFSGFL
                     ITALLIDEFSKTKKIDFVSFCRRRFYRIFPPLVLMVLVTIPFVFLVKSDFRASIGSQI
                     MTALGFTSNFYEILTGGNYESQFIPHLFVHTWSLSIEVHFYVLWGLTVWLLSKRSKDQ
                     KQLRGTLFLVSMGVFGVSFLTMFVRAFFVDNFSTIYFSTLSHIFPFFLGAMVATISGI
                     REITGRFKKNIKNLTLKHNLIMMGSAFAGLMILTFALDFDNRLTYLFGFVLSSIFASV
                     MIYNARILHEHTPDISEPFVITYLADISYGMYLFHWPFYIIFSRLSPNWIAVILTVVL
                     SAVFSTLSFYIIEPFILGRKPKFLDYEFDLLPYKKWLFSIGGVLTLITVVTMLTAPSI
                     GSFETELLQNSLQQARTNTNRTHTLAAGDAGALSDVTVIGDSVALRSSAAFNKLLPEV
                     QLDAAVSRNFSKSFDIFENRIQNKALSKIVVLAVGVNSLDNYKTDLSQFIKSLPKGHR
                     LIIVTPYNAKNMSQVTTVRDYELSLMKKYNYITVADWYKVATEHPEIWGNTDGVHYSD
                     SDTTGADLYVSNVKKAIQKSAQRAAK"
     gene            4723..7365
                     /gene="adhE"
                     /locus_tag="VBE34_000912"
                     /gene_synonym="adhC"
     CDS             4723..7365
                     /gene="adhE"
                     /locus_tag="VBE34_000912"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEO7263640.1"
                     /translation="MTEKTKAVETTDVALAIDTLVQNGLKALDEMRQLNQEQVDYIVA
                     KASVAALDAHGELALHAVEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEEDDV
                     TGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWIEQPSIDATNALMKHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYKEFVEEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKSAVWIAEQAGFTVPEGTN
                     ILAAECTEVSEKEPLTREKLSPVIAVLKAESTEDGVEKARQMVEFNGLGHSAAIHTKD
                     ADLAREFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTDLMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVMTVPGFIAADTGMDVLTHATEAYVSQMANDY
                     TDGLALQAIKIVFDYLERSVKDADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPAATP
                     EEAVESYAKAVYDLGTRLGIKMNFRDQGIDEKEWKEKSRELAFLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYEERPGRRK"
     gene            7544..8560
                     /gene="adhP"
                     /locus_tag="VBE34_000913"
     CDS             7544..8560
                     /gene="adhP"
                     /locus_tag="VBE34_000913"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEO7263641.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPKVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLRVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAIVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIAVYGAGGLGNLAVQYAKKVFNAHVVAVDINADKLQLAKEVGADLTVNGKEIK
                     DVAAYIQEKTGGCHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIRVVGSLVGTRKDLEEAFAFGAEGLVVPVVEKVPVDTAPQVFDEMERGLIQGRKVL
                     DFTK"
     gene            8680..10170
                     /locus_tag="VBE34_000914"
     CDS             8680..10170
                     /locus_tag="VBE34_000914"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEO7263642.1"
                     /translation="MAIVYQSTRDASNKVTASQAILKGLADDGGLFTPITFPKVDLDF
                     TKLKDASYQEVAKLVLSAFFDDFTEQELDYCISQAYDTKFDTTEIAPIVKIGDRYHLE
                     LFHGPTIAFKDMALSILPYLLTTAAKKQGVDDKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKHGVSYIQELQMITQAGQNTHVVAIEGNFDDAQTSVKEMFNNSLLRSKLSQ
                     QHMQLSSANSMNIGRLVPQIVYYIYAYAQLVKSKEISIGQLINFSVPTGNFGNILAAY
                     YASQIGLPVTKLICASNDNNVLTDFFKTQTYDKNREFKVTSSPSMDILVSSNLERLIF
                     HLLGDDAETTKKLMEDLVTTGEYALEARQANILESFVAGFATEQFVELDIKRLFDQYQ
                     YIEDPHTAVASAVYQAYQTETKDQTPTVIVSTASPYKFPCVVTKAITNKEEIQDFAAI
                     SILNDLSGVSLPKAVTDLQKAEVIHRMVVPTSNMRETVEECLGLKQ"
     gene            10275..11555
                     /locus_tag="VBE34_000915"
     CDS             10275..11555
                     /locus_tag="VBE34_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO7263643.1"
                     /translation="MRYSKEIIQLAIPAMIENILQMLMGVVDNYLVAQLGVVAVSGVS
                     VANNIITIYQAIFIALGASIASLLAKSLAGSEKDDAISVCSQAIFLTSLIGAVLGIIS
                     IVFGQTFFKLLGTTKSVAQVGGLYLAIVGGGVVTLGILTTLGSFLRVQGQPRLPMYVS
                     IFVNFLNAVLSGFAIFEWRYGLVGVAVSTLIARLIGICILAKYLPIKKIIKRMTWKIS
                     AQIWNLALPSAGERLMMRAGDVVIVAIVVQLGTNVVAGNAIGETLTQFNYMPGLGIAT
                     ATIILTAKYVGQKNRESIEETIQSSYYIGLVLMILISSFMLLAGKPLTQLFTNNPSAI
                     KGSLIVILLSFVGVPATIGTLVYTAAWQGLGNAKLPFYTTTIGMWLIRVVLGYLLGIV
                     FELGLLGVWMATIADNIFRWLFLKVRYHRYIQKM"
     gene            11764..12072
                     /gene="rpsJ"
                     /locus_tag="VBE34_000916"
     CDS             11764..12072
                     /gene="rpsJ"
                     /locus_tag="VBE34_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEO7263644.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            12145..12771
                     /gene="rplC"
                     /locus_tag="VBE34_000917"
     CDS             12145..12771
                     /gene="rplC"
                     /locus_tag="VBE34_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEO7263645.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVVLIKGNVPGAKKSLITIKSAVKAAK"
     gene            12795..13418
                     /gene="rplD"
                     /locus_tag="VBE34_000918"
     CDS             12795..13418
                     /gene="rplD"
                     /locus_tag="VBE34_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEO7263646.1"
                     /translation="MANVKLFDQTGKEVSSVELNEAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVENLSFAAPKTAEFASVLSALSIDSKVLVILEEGN
                     EFAALSARNLPNVTVATATTASVLDIVNADKLLVTKEAISTIEGVLA"
     gene            13418..13714
                     /locus_tag="VBE34_000919"
     CDS             13418..13714
                     /locus_tag="VBE34_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEO7263647.1"
                     /translation="MNLYDVIKKPVITEKSMVALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVTVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            13732..14565
                     /gene="rplB"
                     /locus_tag="VBE34_000920"
     CDS             13732..14565
                     /gene="rplB"
                     /locus_tag="VBE34_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEO7263648.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTNTPEKSLLVSLKNKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YILAPKGLEVGQRIISGPEADIKVGNALPLANIPVGTVIHNIELQPGKGAELIRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCSATIGTVGNEQQSLVNIGKAGRNRWKGVRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQ
                     K"
     gene            14664..14942
                     /gene="rpsS"
                     /locus_tag="VBE34_000921"
     CDS             14664..14942
                     /gene="rpsS"
                     /locus_tag="VBE34_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEO7263649.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            14958..15302
                     /gene="rplV"
                     /locus_tag="VBE34_000922"
     CDS             14958..15302
                     /gene="rplV"
                     /locus_tag="VBE34_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEO7263650.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKNVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            15315..15968
                     /gene="rpsC"
                     /locus_tag="VBE34_000923"
     CDS             15315..15968
                     /gene="rpsC"
                     /locus_tag="VBE34_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEO7263651.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            15972..16385
                     /gene="rplP"
                     /locus_tag="VBE34_000924"
     CDS             15972..16385
                     /gene="rplP"
                     /locus_tag="VBE34_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEO7263652.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGTPEGWVAPVKRGKV
                     MFEIAGVSEEVAREALRLASHKLPVKCKFVKREAE"
     gene            16395..16601
                     /locus_tag="VBE34_000925"
     CDS             16395..16601
                     /locus_tag="VBE34_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEO7263653.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            16627..16887
                     /gene="rpsQ"
                     /locus_tag="VBE34_000926"
     CDS             16627..16887
                     /gene="rpsQ"
                     /locus_tag="VBE34_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEO7263654.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            16912..17280
                     /gene="rplN"
                     /locus_tag="VBE34_000927"
     CDS             16912..17280
                     /gene="rplN"
                     /locus_tag="VBE34_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEO7263655.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            17360..17665
                     /gene="rplX"
                     /locus_tag="VBE34_000928"
     CDS             17360..17665
                     /gene="rplX"
                     /locus_tag="VBE34_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEO7263656.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVVVEGVALIKKH
                     QKPNNENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYNKKSGEVLD"
     gene            17689..18231
                     /gene="rplE"
                     /locus_tag="VBE34_000929"
     CDS             17689..18231
                     /gene="rplE"
                     /locus_tag="VBE34_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEO7263657.1"
                     /translation="MANRLKEKYTNEVVPALTEKFNYSSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            18249..18434
                     /locus_tag="VBE34_000930"
     CDS             18249..18434
                     /locus_tag="VBE34_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEO7263658.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCEKCGRPHSVYRKFQLCRVCF
                     RDLAYKGQVPGVTKASW"
     gene            18589..18987
                     /gene="rpsH"
                     /locus_tag="VBE34_000931"
     CDS             18589..18987
                     /gene="rpsH"
                     /locus_tag="VBE34_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEO7263659.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIADILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGQNGERVITNLKRISKPGLRVYTKHEDMPKVLNGLG
                     IAIVSTSEGLLTDKEARQKNVGGEVLAYIW"
     gene            19097..19633
                     /gene="rplF"
                     /locus_tag="VBE34_000932"
     CDS             19097..19633
                     /gene="rplF"
                     /locus_tag="VBE34_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEO7263660.1"
                     /translation="MSRIGNKVITLPAGVEIINKDNVVTVKGPKGELTREFNKNIGIT
                     VEGTEVTVTRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKALEMRGVGYRAQLQGSK
                     LVLSVGKSHQDEVEAPEGVTFEVPTPTTINVIGINKESVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            19734..20090
                     /gene="rplR"
                     /locus_tag="VBE34_000933"
     CDS             19734..20090
                     /gene="rplR"
                     /locus_tag="VBE34_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEO7263661.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNIFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSNGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADSARENGLKF"
     gene            20109..20603
                     /gene="rpsE"
                     /locus_tag="VBE34_000934"
     CDS             20109..20603
                     /gene="rpsE"
                     /locus_tag="VBE34_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEO7263662.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDRNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMVEVPMVGTTIPHEVRSEFGGAKVLLKPAVEGA
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            20618..20797
                     /gene="rpmD"
                     /locus_tag="VBE34_000935"
     CDS             20618..20797
                     /gene="rpmD"
                     /locus_tag="VBE34_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEO7263663.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVNAISHLVTVEEA"
     gene            20922..21362
                     /gene="rplO"
                     /locus_tag="VBE34_000936"
     CDS             20922..21362
                     /gene="rplO"
                     /locus_tag="VBE34_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEO7263664.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRMPKRGFSNINAKEYALVNLDQLNVFEDGTEVTPVVLKEAGIV
                     RAEKSGVKILGNGELTKKLSVKAAKFSKSAEAAITAKGGSIEVI"
     gene            21383..22687
                     /gene="secY"
                     /locus_tag="VBE34_000937"
     CDS             21383..22687
                     /gene="secY"
                     /locus_tag="VBE34_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEO7263665.1"
                     /translation="MFLKLLRDALKVKMVRNKILFTIFILLVFRIGTHITVPGINVKS
                     LEQMGELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLFLAFVQSIGITAGFNTLSSVALVKTPNVQTYLLIGAILTTG
                     SMVVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAITTIYEDFFVNVRSSAITNSYIF
                     VGILIVAVLAIVFFTTFIQQAEYKIPIQYTKLVQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFFQNGKEIPWLTKLQELLNYQTPVGMIIYAILIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSIRPGRETEEYMSSLLKKLATIGSVFLAFISLLPIIAQQALHL
                     SSSIALGGTSLLILIATGIEGMKQLEGYLLKRRYVGFMNTTE"
     gene            22782..23420
                     /locus_tag="VBE34_000938"
     CDS             22782..23420
                     /locus_tag="VBE34_000938"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEO7263666.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTQ
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPTCLIERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPILEHYRKLGLVTDIEGNQEITEVFADVEKALLELK"
     gene            23536..23754
                     /gene="infA"
                     /locus_tag="VBE34_000939"
     CDS             23536..23754
                     /gene="infA"
                     /locus_tag="VBE34_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEO7263667.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            23780..23896
                     /gene="rpmJ"
                     /locus_tag="VBE34_000940"
     CDS             23780..23896
                     /gene="rpmJ"
                     /locus_tag="VBE34_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEO7263668.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            23914..24279
                     /gene="rpsM"
                     /locus_tag="VBE34_000941"
     CDS             23914..24279
                     /gene="rpsM"
                     /locus_tag="VBE34_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEO7263669.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLSTSKKILAAAGISEDIR
                     VKDLTPDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            24297..24680
                     /gene="rpsK"
                     /locus_tag="VBE34_000942"
     CDS             24297..24680
                     /gene="rpsK"
                     /locus_tag="VBE34_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEO7263670.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            24730..25668
                     /locus_tag="VBE34_000943"
     CDS             24730..25668
                     /locus_tag="VBE34_000943"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEO7263671.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELDVEGP
                     AEITAGDILTDSDIEIVNPDHYLFTIAEGHSLKATMTVAKNRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKTEPEMMKVRNLGRKSLEEVKIKLADLGLGLKNDK"
     gene            25683..26069
                     /gene="rplQ"
                     /locus_tag="VBE34_000944"
     CDS             25683..26069
                     /gene="rplQ"
                     /locus_tag="VBE34_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEO7263672.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEASDKYTSTTALQKLFDDIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
BASE COUNT         8479 a         4481 c         5447 g         7969 t
ORIGIN      
        1 acttgtgcag ttgtatctaa atttaacaat agagcattta ctaaagaagt atttcaaact
       61 atatgtaaaa ctttgataag tcagaaccac aaacttacag cagaagattt gttttggttt
      121 aatcatgtac tcctgaattg tgtatttgtt ggactttgtt taaatagtga agagtgtctt
      181 gcagaaatgt tagaagttag ccgacaaacg atggtgtcaa cacatgattt tcataaaatg
      241 cctttatatt ttatgtacca atggaaatac ttcattacaa ttgacaatga tataaagtct
      301 gcagaaaatg cttaccaaca atcaattatg ttttcaaaaa tgattgatga caagcatcta
      361 attaaaaaat tagaattgga gtggcaagaa gatataacag gacattgatg tcctgtgact
      421 ctgaggtcta aaaatttata ataaacgtac aaaatattat ttaggagttt attatagtga
      481 ttaaaaaatg ttttttaata gtattacttg ttctattatc aaatttaggt tcatttgatt
      541 tgtttttagg aatgggttgg tggaatatgg gctaagtaag ctgttctgtg atttcttgag
      601 gttggattaa ctataagatc agtttattct tcctttaaat aagggagata ctaatacacc
      661 aacttagttc ttaacttttc ctatacaaat gttataataa tatcatgaca agatttttag
      721 atagtgatgc aatgggtgac gaagaattgg tagaacgtac acttcgtccg cagtatttaa
      781 gagagtatat tggacaagat aaggttaaag atcagctaaa aatatttatt gaagctgcta
      841 aattgcgtga tgagtcattg gatcatgtgt tattatttgg ccctcctggt ttagggaaaa
      901 caaccatggc atttgtaatt gctaatgagt tgggtgtcaa tctcaaacaa acatcaggtc
      961 ccgcaattga aaaatcaggg gatttagtag ccattttaaa tgatttagaa ccaggtgatg
     1021 ttctttttat tgatgaaatt catcgtatgc cgatggcggt tgaagaggta ctttatagtg
     1081 caatggaaga cttctatatt gacattatga tcggtgcagg agaaactagt agaagtgttc
     1141 atttagattt gccgcccttt accttaattg gtgcaacgac acgtgcaggt atgttatcta
     1201 atcccttacg tgctcgcttt ggtattacag ggcatatgga gtattatgaa gaaaacgatt
     1261 tgacagaaat tattgagcgt acagcagata tttttgaaat gaaaattact tatgaagctg
     1321 cttctgaatt agcgcgtcgc agtcgtggaa cgccacgtat cgctaaccgt ttattgaaac
     1381 gtgttcgaga ttatgctcaa atcatgggag atggtttgat agatgacaat attacagata
     1441 aagcattaac gatgttagat gttgatcacg aggggcttga ttacgtcgat caaaaaatct
     1501 taagaaccat gattgaaatg tataatggag gtcctgttgg tttaggaact ctatccgtta
     1561 atattactga agaacgagat actgttgaag acatgtacga accttattta attcaaaaag
     1621 gttttattat gcgtacccgt accggtcgtg tagctacgga taaggcatac gaacatttag
     1681 gttatcagcg atttgataaa taaaacataa gtcattttaa ggctagggta tctagtctta
     1741 attttttaat tttcatgcaa tgttaagtat tagatatgtt gtagttattc tttgagcagt
     1801 taaacgtatt ataagtacct tttttctgtt ataatagacc aaaaggagtt tgctatgaag
     1861 aaagtctgtt ttgtttgtct tggaaatatc tgccgtagcc ctatggctga gtttgttatg
     1921 gcgaatttag ataaggagga tagcctctcg gttgagagtc gcgcgacttc aacatgggag
     1981 catggaaatc ctatacatag gggaacgcaa gcgattttgc agcgttatca aattccttac
     2041 actaacgata aagggtcaca acctatttct aaagacgatt tagcatattt tgattatatc
     2101 atcggtatgg atgagcaaaa tgtcaaggat ttaaaagctc tatcacaagg aacctatgat
     2161 gataagatta gccttttttg tgatggtggt gttcctgatc catggtatac tggtgatttt
     2221 gaggaaacct atcgtctagt cagtgatggc tgtaaacagt ggttaacgct aataaataat
     2281 agtagagatt aagtagaaat gaagaatttt aaaataacac gaactcatct agaaatatta
     2341 tctttgataa ttattgttgt ttttggttta tcagtattta ccttaacgac ttctagtcaa
     2401 ggagtcctta gctatgatgg cggtaagata aaatatgtgg gctctattgt taatcaccat
     2461 atgactggta aaggtaaatt aacctatgag aatggggatt actataaggg cgattttgta
     2521 aatggggtct ttgaagggaa aggaacattt gtttcagttc atggctggtc ttatacaggt
     2581 gactttaaaa aaggtcaacc tgatggacaa ggtcgcctca atgctaaaaa taagaaagtc
     2641 tataagggga catttaaaca ggggatttat caaaaatgag aataaaatgg ttttctttgg
     2701 tacgtataac aggactacta cttgttttac tttaccattt ctttaagaat agttttccag
     2761 gaggatttgt tggtgttgac atcttcttta cattttctgg ttttctgatt acggccttgt
     2821 tgattgatga attttctaaa acgaaaaaaa ttgattttgt tagtttttgt cgtagacgtt
     2881 tttaccgtat tttcccacca ttagtattga tggttttggt gacaatacct tttgtatttc
     2941 tggttaaaag cgattttaga gcaagtattg gctctcaaat tatgactgct ttaggtttta
     3001 ccagcaactt ttacgaaatc ttgactggtg gtaactacga aagtcaattt atccctcact
     3061 tatttgtgca tacttggagt ctttctattg aagtccattt ttatgtttta tggggcttaa
     3121 cggtatggct cttatcgaaa agatcaaaag atcaaaagca attgaggggg actttgttcc
     3181 ttgtttccat gggagttttt ggagtcagct tccttaccat gtttgttaga gcctttttcg
     3241 ttgataattt ttcaacgata tatttctcta cattatcaca tatatttccg ttctttttag
     3301 gagcgatggt ggctacaata tctggtattc gagagattac cggacgattc aagaaaaata
     3361 tcaaaaatct gactttgaag cataatttaa tcatgatggg tagtgctttt gctggtctaa
     3421 tgattttaac ttttgcactt gattttgata atcggctaac atatcttttt ggttttgttt
     3481 tgtctagcat ttttgctagt gtaatgattt acaatgcacg tattttacac gagcatacac
     3541 cagatatttc agaacctttc gtgattactt atcttgctga tatcagttac ggtatgtacc
     3601 tttttcattg gccattttat attattttta gtcgtttatc cccaaactgg atagctgtta
     3661 ttttgacagt agtattatca gcagtcttct caacattatc attttatatc attgaacctt
     3721 ttattttagg tagaaaacct aaattccttg attatgaatt tgacttgttg ccttacaaaa
     3781 agtggttatt tagtatcgga ggggtactaa ctcttattac tgtagtgaca atgttgacag
     3841 ctccaagtat aggaagtttt gagacagaat tattgcagaa ttcgttacaa caagctagaa
     3901 caaatactaa ccgaacacat acactagccg caggggatgc aggtgctctt agtgatgtga
     3961 cggtaatcgg agattcagtt gccttaaggt caagtgctgc ttttaataaa ttgttaccag
     4021 aagtacaatt agatgctgcg gtgagtcgta attttagtaa atcatttgat atttttgaaa
     4081 accgtattca aaataaagcc ttgtccaaaa ttgttgtttt ggcagttggt gtgaattcat
     4141 tagataacta caagacagac ttgagccaat ttattaaatc tttgcctaag ggacaccgtt
     4201 tgattatagt aacgccttat aatgctaaaa atatgagtca agttacgaca gtgcgtgatt
     4261 atgaactctc gctaatgaaa aaatataatt acataacggt tgctgactgg tataaggttg
     4321 cgacagaaca tccagaaatc tggggaaata cagatggtgt gcattatagt gatagcgata
     4381 ctacaggggc tgatttatat gttagtaacg tcaaaaaagc tattcaaaaa tcagctcaac
     4441 gagctgcaaa ataaacaagt ccgagtcatg tactcggctt ttttgtgaaa acgaataata
     4501 gatacctccg tttatggtga tacaaatgaa atcgattggc tttaatctga aaattagtaa
     4561 tgaaaaaaca aatttaatta aagcgctttc aagaaatgtt tccgaaagat tgaaaaaaag
     4621 ttcacaaaaa agttgtgaaa ctatttacaa agtttttaaa tatgctataa taatctcgta
     4681 aacaaatttg tgaaacattt aacaaatcaa aaggagtctc aaatgactga aaaaactaaa
     4741 gcggtagaaa ctacagatgt tgctcttgca attgatacat tagttcaaaa cggtttaaaa
     4801 gcacttgatg agatgcgtca acttaatcaa gaacaagttg attatattgt agctaaagct
     4861 tctgttgcag ccttggatgc ccatggagaa cttgctcttc atgcagtaga agaaactggt
     4921 cgtggtgtct ttgaagataa agcgacaaaa aatttatttg catgtgaaca cgtcgttaac
     4981 aacatgcgtc atacaaagac agtgggagtt attgaagaag acgatgtcac aggcttgaca
     5041 ttaattgctg aaccagtggg agttgtctgt ggtattacgc caacaaccaa cccgacttca
     5101 actgctattt tcaagtcatt aatctctctt aaaacacgta atccaattat ttttgccttc
     5161 cacccatcag cacaagaatc atcagcacat gcagcacgta ttgttcgtga tgctgctatc
     5221 gctgctggag ctcctgaaaa ttgtgtccaa tggattgaac aaccatcgat tgatgcaact
     5281 aatgctttga tgaaacatga tggtattgcc actatccttg cgacaggtgg taatgcgatg
     5341 gttaaagctg cctactcatg tgggaaacct gcccttggtg taggtgctgg gaatgttcca
     5401 gcttatgttg aaaaatcagc taatatccgt caagctgctc acgatattgt tatgtctaaa
     5461 tcatttgata acggtatggt atgtgcgtca gaacaggctg ttattattga taaagaaatt
     5521 tataaagaat ttgttgaaga atttaaatct tatcatacct atttcgttaa taagaaagaa
     5581 aaagctcttc ttgaagaatt ctgttttggt gcaaaagcaa atagcaaaaa ctgtgctggt
     5641 gcaaaattaa atccaaatat tgttggaaaa tcagcagttt ggattgctga acaagctgga
     5701 tttactgttc cagaaggaac aaatattctt gccgcagaat gtactgaagt tagtgaaaaa
     5761 gaacctttga cacgtgaaaa actatcacct gttattgcag tattgaaagc tgaatctaca
     5821 gaagatggtg ttgaaaaagc tcgtcaaatg gttgagttta atggtctagg gcactcagct
     5881 gctattcata caaaagatgc agacttagct cgtgaatttg gaactagaat tcgtgctatc
     5941 cgtgttattt ggaattctcc ttctactttt ggtggtattg gggatgttta caatgctttc
     6001 ttgccatcat tgactcttgg ttgtggttcg tatggacgta actcagttgg tgataacgtt
     6061 agtgctataa atctcttgaa tatcaaaaaa gtaggaagac gtagaaataa tatgcaatgg
     6121 tttaaagtcc cttcaaaaac atacttcgaa cgtgattcta ttcaatattt gcaaaaatgt
     6181 cgcgatgttg aacgtgtcat gattgttaca gatcacgcca tggttgagct tggtttcttg
     6241 gatcgtatca ttgaacaact tgatcttcgt cgcaataaag ttgtttatca gatttttgct
     6301 gaagtagaac cagatccaga cattacaaca gtaatgaaag ggactgattt aatgcgtacc
     6361 ttcaaaccag acactattat tgcattaggt ggtggttctc ctatggatgc tgcgaaagtt
     6421 atgtggctct tctatgaaca accagaagtt gatttccatg acctcgttca aaaattcatg
     6481 gatatccgta aacgtgcgtt caaattccca gaacttggta agaaaacaaa atttgttgct
     6541 attccaacaa cttctggaac aggttctgaa gtgacaccat ttgccgttat ctctgacaaa
     6601 gcaaataatc gtaaatatcc aatcgctgat tattcattaa caccaactgt agctatcgtt
     6661 gatccggccc ttgttatgac tgttcctggt tttattgcgg ctgatacggg tatggatgta
     6721 ttgacacatg caacagaggc ttatgtttca cagatggcaa atgattatac agacggactt
     6781 gctcttcaag caattaaaat tgtatttgac tacttagaac gctcagtaaa agatgctgat
     6841 tttgaagcac gtgaaaagat gcacaatgcg tcaactatgg caggtatggc tttcgccaat
     6901 gcattcttag gtatttctca ctcaatggcg cataaaattg gcgcacaatt ccataccgta
     6961 cacggacgta caaatgcaat tcttttgccg tatgttatcc gctataatgg aactcgtcca
     7021 gctaaaacag caacatggcc taagtacaat tactatcgtg ctgatgaaaa gtatcaagat
     7081 atcgctaaat tactcggctt accagcagca acaccagaag aagctgtcga atcatatgca
     7141 aaagctgtgt atgaccttgg aacacgttta ggtattaaga tgaatttccg tgaccaaggc
     7201 attgatgaaa aagaatggaa agaaaaatct cgtgaactag ctttcttagc atatgaagat
     7261 caatgttcac cagcaaatcc acgccttcca atggtagatc acatgcaaga aatcatcgaa
     7321 gatgcttatt atggttatga ggaacgtcca ggacgccgta agtaacaaca gtcgaagaat
     7381 ctcactctat tgagtgggat ttttagtttc aaaaagatta accagttaat aatattttca
     7441 aaaaattttc tgaaaatcac ttgacatagt ttgtgaataa taatacaata taaaaatgta
     7501 aacgattaca aataagatat atttatttct aggaggtttt atcatgaaag cggttgttgt
     7561 taatcaagct agtacaggtg ttgaagttgt tgagcatgat cttccaaaag ttggtcatgg
     7621 tgaagcactt gtaaaagtag aatactgtgg tgtgtgccat actgatttac acgttgcaca
     7681 tggagatttc ggccaggtac caggacgcat tttgggtcac gaaggcattg gtatagtaga
     7741 agaaattgga gaaggcgtaa cgtctttgag ggttggtgat cgtgtctcta ttgcatggtt
     7801 ctttgaaggg tgcggtcatt gcgaatactg tactacagga cgtgagacgc tttgtcgtag
     7861 tgttaaaaat gctggataca gtgttgatgg tggtatgagt gaatacgcta tcgttaccgc
     7921 ggactatgcg gttaaggttc ctgagggatt agatccagct caagcatcat caatcacttg
     7981 tgctggagta acaacataca aggctatcaa agaagctgga gctgctcctg gtcagtggat
     8041 tgcagtgtat ggtgcaggtg gtcttggaaa cttagcagtc caatatgcaa aaaaagtatt
     8101 caatgctcat gttgtagctg ttgatattaa cgcagataaa cttcaattag ctaaagaggt
     8161 tggagcagat ttgacagtta atggcaaaga aataaaagat gttgcggcct atattcaaga
     8221 aaagacaggc ggttgtcatg gagtggttgt gactgctgtt tctaaagttg cgtttaatca
     8281 ggcgattgac agcgttcgtg ctggaggtac agtggtagct gttggtcttc catcagaata
     8341 tatggaattg agtattgtaa aaacagtact tgatgggatt cgtgtggttg gttccttagt
     8401 tggaactcgt aaagatttag aagaagcctt cgcatttgga gcagaaggtt tggtagtacc
     8461 agtggttgaa aaagtgcctg tggatacagc tcctcaagta ttcgatgaaa tggaaagagg
     8521 attgattcaa ggacgtaaag ttctcgactt tacaaagtga taatgatatt acgcccccct
     8581 ttattttaat aaaggggggt taattatata acgaacaatg gacatgttat taaattgtgt
     8641 tacaataact aaaagatatt tatataaaga gggattctta tggcaatagt atatcaatca
     8701 acacgtgatg ctagtaataa agtgacagct agtcaagcga ttctaaaggg attagctgac
     8761 gatggaggac tctttacccc catcacattt ccaaaagttg atttagattt cactaaactt
     8821 aaggatgctt cctaccaaga agtggctaaa ctcgttttgt cagctttttt tgatgatttt
     8881 actgaacaag aattggatta ctgtattagt caagcttatg atactaaatt tgatacaact
     8941 gaaatagccc caatagtaaa gattggagac cggtatcacc ttgaattatt tcacggtcct
     9001 acaattgcat ttaaagatat ggctttatcc attcttcctt atttgctaac aacagcagcc
     9061 aaaaaacaag gggttgatga taaaatcgtt attttaacag caacatcagg tgacactggt
     9121 aaggcagcga tggcaggttt tgcagatgtt ccaggaactg agattattgt attttatcct
     9181 aaacatggtg ttagttatat tcaagaattg cagatgataa cacaagccgg gcaaaatacc
     9241 catgttgttg cgattgaagg aaattttgat gatgcacaga cttcggttaa agaaatgttc
     9301 aataattccc ttcttcgatc aaaattatca cagcagcata tgcagttatc atcagccaat
     9361 tcaatgaaca tcggtcgcct tgttcctcaa attgtttact atatatatgc atatgctcaa
     9421 ttagttaagt caaaggaaat ctcaattggt caactaatta attttagtgt acctacagga
     9481 aattttggta atattttagc agcatattat gctagtcaaa ttggattacc tgttaccaaa
     9541 ctcatttgtg cctcaaatga taataacgtt ctaacagatt tttttaagac acaaacctac
     9601 gataaaaatc gagaatttaa agtgacatca agtccctcta tggatatttt ggtctcctct
     9661 aatctagaac gattaatctt tcacctttta ggcgatgatg cagaaacgac taaaaaatta
     9721 atggaagatt tagtgactac tggggagtat gccttagagg ctaggcaagc aaatatttta
     9781 gagagctttg tagcgggatt tgcgacagaa caatttgttg aattagacat caaacgttta
     9841 tttgaccagt atcagtatat agaggatccg catacagctg tagcatcagc tgtttatcaa
     9901 gcttatcaga cagaaactaa agatcaaact ccaacagtaa ttgtgtcaac agcaagtcct
     9961 tataagttcc cgtgtgttgt tacaaaggct attacaaata aagaagagat acaggatttc
    10021 gctgctattt caatcttaaa cgatttgtca ggtgtttctt tacctaaggc ggttacagat
    10081 ctacaaaagg ccgaagtaat ccatagaatg gtagtaccta cctcaaatat gcgagaaacg
    10141 gttgaagagt gtctaggttt gaaacaataa taaaaatacg attgttttaa aatatttaga
    10201 gcgagaaaga aacattttag aaattttccc atcactctct ttttaatgat cctgggtaat
    10261 agaaaggaga tactgtgcgt tatagtaaag agattattca gttagctata ccagctatga
    10321 ttgaaaatat cttacaaatg ctcatgggag tagttgataa ttatctagtg gctcagttag
    10381 gtgttgtagc agtatcaggt gtttcagttg ctaataatat aattactatt tatcaagcta
    10441 tttttatagc tttaggggcg agtatagcaa gtctattggc caagtcgtta gcaggtagtg
    10501 agaaggatga tgcaatttca gtatgttctc aagccatttt tctaacatca ctgatagggg
    10561 cagtattagg aattatctcg attgtttttg gacaaacttt ctttaaactt ttagggacaa
    10621 caaaaagtgt cgcacaagtt ggtggtttat atttagcaat tgtgggtgga ggtgttgtta
    10681 ctttaggaat attaaccacc ctaggctcat ttttacgtgt gcaagggcaa cctcgtttac
    10741 caatgtatgt cagtattttt gtcaatttcc tcaatgcagt cttatcaggt tttgctattt
    10801 ttgagtggcg ctatggtttg gtcggagtgg cagtatcaac tcttatcgca agattaattg
    10861 gtatttgtat ccttgcgaag tatcttccta ttaaaaaaat aataaaaaga atgacttgga
    10921 aaatcagtgc acaaatttgg aatcttgcct taccatcagc aggagaacgt ctgatgatgc
    10981 gagctggaga tgttgttata gttgctattg ttgttcagtt aggtacaaat gttgtagcgg
    11041 gaaatgctat tggagaaacc ttgacacaat ttaattatat gccaggactt ggcatcgcaa
    11101 ctgctacaat tatcctaact gcaaaatatg taggacaaaa aaatagagaa tctatagaag
    11161 aaactattca aagttcgtat tatattggct tagttctaat gatccttata tcaagtttta
    11221 tgcttcttgc cggcaaacca ttgacacagt tatttacaaa taatccttct gccattaaag
    11281 gaagtttgat tgttattttg ctatctttcg taggagtacc tgctactatt gggaccttgg
    11341 tttatacagc agcttggcaa ggattaggaa atgctaaact cccattttac acaacgacta
    11401 taggcatgtg gttaatcaga gtagttcttg gttatttatt aggaatagtt tttgaattag
    11461 gtttgcttgg ggtttggatg gctacgatag cagataatat ctttaggtgg ctgtttttga
    11521 aagttcgcta tcatcgatat attcaaaaaa tgtaaccaga aatggttgca ttttttttga
    11581 aaaaaaaaat aaaataatgc ttgcgtgtgc tgtttttttt tgatatcata tactggtgct
    11641 gtgtatgaac agctttagct atgatgcaag aggttgcgac acgctaggtt gcattgccac
    11701 gcaactcgtg ttggttttct tgaggagcta gcctattatc gtaaatagac gagaggagaa
    11761 aagatggcaa acaaaaaaat ccgtatccgt ttgaaagcgt acgaacaccg tacacttgat
    11821 acagcggcag aaaaaatcgt tgaaactgca acacgtacag gtgctacagt tgctggacca
    11881 gttccgcttc caactgaacg cagtctttac acaattattc gtgcgactca caaatataaa
    11941 gattctcgcg aacagtttga aatgcgtaca cacaaacgtt tggtagacat catcaaccca
    12001 acacaaaaaa ctgttgatgc tttgatgaaa cttgatcttc caagtggtgt caacgtagaa
    12061 atcaaacttt aatttgattt ctgagcacaa aaaacgctcg taaaaaactt tttgaaataa
    12121 aataagaaaa ggaaatattt tctcatgaca aaaggaatct tagggaaaaa agtgggaatg
    12181 actcaaatct tcactgaatc aggcgaattc atccctgtta ctgtcattga agcaactcca
    12241 aacgttgtgc ttcaagttaa aactgttgaa acagacggtt acgaagcagt tcaggttggt
    12301 tttgatgaca aacgtgaagt attgagtaac aaacctgcca aaggccatgt tgcaaaagca
    12361 aacacagctc ctaagcgctt cattcgtgaa ttcaaaaaca ttgaaggctt agaagttggt
    12421 gcagaattat ctgtagaaca atttgaagct ggtgatgttg ttgacgtcac agggacatca
    12481 aaaggtaaag gtttccaagg tgttatcaaa cgccatggtc aatcacgtgg tcctatggct
    12541 cacggttctc gttaccatcg ccgtccaggt tctatgggac ctgttgcgcc taaccgcgtt
    12601 ttcaaaaaca aacgcttggc aggacgtatg ggtggtaacc gtgtaacagt tcaaaacctt
    12661 gaaattgtac aagttatccc agagaaaaac gttgtcctca tcaaaggtaa cgtaccaggt
    12721 gctaagaaat ctcttatcac tatcaaatca gcagttaaag ctgctaaata ataagaaagg
    12781 agaaaacagt taaaatggca aacgttaaac tatttgacca aactggtaaa gaagttagct
    12841 cagttgagct taacgaagct atcttcggta tcgaaccaaa cgaatcagtt gtttttgatg
    12901 ttgtaatcag ccaacgcgct agccttcgcc aaggtactca cgcggttaaa aaccgttcag
    12961 cagtatctgg tggtggacgt aaaccatggc gtcaaaaagg aactggacgc gctcgtcaag
    13021 gttctatccg ctcaccacaa tggcgtggtg gtggtgttgt ctttggacca actccacgtt
    13081 catacggcta caaacttcca caaaaagttc gtcgccttgc gttgaaatca gtttattcag
    13141 caaaagttgc tgaagataaa tttgtagctg tagaaaacct ttcatttgca gctccaaaaa
    13201 ctgctgaatt tgcgtcagtt ctttcagcac ttagcattga ttcaaaagta cttgtaatcc
    13261 ttgaagaagg taacgaattt gctgcacttt cagcgcgtaa ccttccaaac gttacagttg
    13321 ctactgcaac tactgcaagt gttcttgata tcgttaacgc agacaaactt cttgttacta
    13381 aagaagcaat ctctacaatc gagggggttc ttgcataatg aatttgtatg acgtaatcaa
    13441 aaaacctgtt atcactgaga aatcaatggt tgctcttgaa gcagggaaat atacatttga
    13501 agttgacaca cgtgcacaca aacttttgat caaacaagct gttgaagctg catttgacgg
    13561 agtaaaagtt gcaagtgtaa acactgtgac agttaaaccc aaagcaaaac gtgttggtcg
    13621 ttacacaggt tttacttcaa aaactaaaaa agctattatc actcttacag ctgattcaaa
    13681 agcaatcgag ttgtttgctg ctgaagctga ataatctaag gaggaaataa cgtgggtatt
    13741 aaagtttata aaccaacgac aaatggccgt cgtaatatga cttctttgga ttttgcagaa
    13801 atcactacaa acactcctga gaaatcattg cttgtttcac taaagaataa agcaggacgt
    13861 aacaacaacg gacgtattac tgttcgtcac caaggtggtg gacacaaacg tcattaccgt
    13921 ttgattgact tcaaacgtaa taaagatggt gttgaagcag ttgttaaaac aattgaatac
    13981 gatccaaatc gtactgcaaa tattgctctt gtacactata ctgatggtgt taaagcttac
    14041 atccttgcgc ctaaaggcct tgaagtaggt caacgcatta tttctggtcc agaagcagac
    14101 atcaaagttg gtaacgcact tccgcttgct aacattccag tcggtacagt tatccataat
    14161 attgaattgc aaccaggtaa aggtgctgaa ttgattcgtg ccgctggtgc ttctgctcaa
    14221 gtacttggtc aagaaggtaa atatgttctt gttcgccttc aatcaggtga agtacgtatg
    14281 atccttggta cttgcagtgc aacaattggt actgttggta atgaacagca atcacttgtt
    14341 aacattggta aagcaggacg taatcgttgg aaaggtgttc gccctacagt tcgtggttct
    14401 gtaatgaacc ctaacgatca cccacacggt ggtggtgaag gtaaagcacc agttggtcgt
    14461 aaagcaccat ctactccatg gggtaaacct gcgcttggac ttaaaactcg taataagaaa
    14521 gctaaatcag acaaacttat tgttcgtcgt cgtaaccaaa aataataggt taatagcata
    14581 atcttataca aatccgccag ctcggtagta cactatagtg tacaggccgt tgtggtacat
    14641 tatttaaagg agaaaactac aaaatgggac gtagtcttaa aaaaggacct ttcgtcgacg
    14701 agcatttaat gaaaaaagtt gaagctcaag caaatgacga aaagaaaaaa gtaatcaaaa
    14761 cttggtcacg tcgttcaacg attttcccaa gtttcatcgg atacacaatc gcagtttatg
    14821 atggacgtaa acatgttcct gtttatatcc aagaggacat ggtaggtcac aaacttggtg
    14881 aatttgcacc aacacgtact tacaaaggtc acgcagctga cgacaagaaa acacgtcgtt
    14941 aataggagga ggacacaatg gcagaaatta cttcagctaa agcaatggct cgtacagtgc
    15001 gtgtttcacc tcgtaaaaca cgtttagtac ttgatcttat ccgtggtaag aatgttgctg
    15061 acgcaatcgc aatcttaaaa ttcactccaa acaaagcagc tcgtgttatt gagaaaactc
    15121 ttaactcagc aattgcaaat gcagaaaaca actttggttt ggaaaaagca aacttggtag
    15181 tatctgaaac tttcgctaac gaaggaccaa caatgaaacg tttccgtcca cgtgcgaaag
    15241 gttcagcttc accaatcaac aaacgtacaa ctcacgtaac tgtagttgta tcagaaaaat
    15301 aaggaggtaa aatcgtgggt caaaaagtac atccaattgg tatgcgtgtc ggaatcatcc
    15361 gtgactggga tgcgaaatgg tatgctgaaa aagaatacgc ggattacctt catgaagatc
    15421 ttgcaatccg taaattcatt aataaagaat tagctgacgc atcagtttca actattgaaa
    15481 ttgaacgtgc agtaaataaa gttattgttt cacttcacac tgctaaacca ggtatggtta
    15541 tcggtaaagg tggagcaaac gttgacgctc ttcgtggtca acttaacaaa ttaactggaa
    15601 aacaagtaca catcaacatc attgaaatca aacaacctga tttagatgct caccttgttg
    15661 gtgaaaatat tgctcgtcaa ttagagcaac gtgttgcttt ccgtcgtgct caaaaacaag
    15721 caatccaacg tacaatgcgt gcaggagcta aaggtattaa aactcaagtt tctggtcgtt
    15781 tgaacggtgc tgatatcgct cgtgctgaag gttattcaga aggaactgtt ccacttcaca
    15841 cgcttcgtgc tgatatcgac tatgcttggg aagaagctga cacaacttat ggtaaacttg
    15901 gcgttaaagt ttggatttac cgtggagaag ttcttccagc tcgtaaaaac actaaaggag
    15961 gcaaataaca aatgttagta cctaaacgtg ttaaacaccg tcgcgaattc cgtggaaaaa
    16021 tgcgcggtga ggctaaaggt ggtaaagaag tttcattcgg tgaatacgga cttcaagcta
    16081 caactagcca ctggattaca aaccgtcaaa tcgaagctgc ccgtattgcg atgactcgtt
    16141 acatgaaacg tggtggtaaa gtttggatta aaatcttccc acacaaatca tacactgcaa
    16201 aagctatcgg tgtacgtatg ggttctggta aagggacacc tgaaggttgg gtagcaccag
    16261 ttaaacgtgg taaagtgatg tttgaaattg ctggtgtttc tgaagaagtt gcacgtgaag
    16321 cattacgtct tgctagccac aaattaccag ttaaatgcaa attcgtaaaa cgtgaagcag
    16381 aataaggaga agacatgaaa cttcaagaaa ttaaagattt tgttaaagag cttcgtggat
    16441 tgtctcaaga agaacttgct aagaaagaaa acgaactcaa aaaagaattg tttgatcttc
    16501 gtttccaagc tgcagcaggt caacttgaaa agactgctcg ccttgacgaa gttaagaaac
    16561 aaattgcacg tgttaagact gtgcaatcag aaatgaaata atagattggg aaaggagaaa
    16621 ttctaaatgg aacgtaatca acgtaaaact ctttacggac gcgtagtgtc tgacaagatg
    16681 gacaaaacaa tcacagttgt agttgaaact aaacgtaacc acccagtcta tggtaaacgt
    16741 atcaactatt ctaaaaaata taaagcacat gatgaaaaca acgttgctaa agaaggcgat
    16801 atcgttcgta tcatggaaac tcgcccactt tcagctacaa aacgtttccg tcttgtggaa
    16861 gtagtggaaa aagctgttat tatctaatca aactaaaagg agaaaattga aatgattcaa
    16921 caagaaactc gcttgaaagt tgctgataat agcggtgctc gtgagatctt gactatcaaa
    16981 gtacttggtg gttcaggacg taaattcgct aacatcggtg acgtaatcgt tgcttctgta
    17041 aaacaagcta ctcctggtgg agcagttaaa aaaggtgatg tggttaaagc tgttatcgtt
    17101 cgtacaaaaa ctggtgcacg ccgcccagac ggttcataca tcaaatttga cgacaatgct
    17161 gctgtaatca tccgtgacga taaaactcct cgcggaactc gtatctttgg cccagttgca
    17221 cgtgaattgc gtgaaggtgg ctacatgaag atcgtttcat tggcaccaga agtactttaa
    17281 tctcagatta acaaacgtag tcccctagga tttcctaggg tgcctttcga ggcgtaagaa
    17341 aaataggaga aaaactcaaa tgtttgtaaa aaaaggcgac aaagttcgcg ttattgctgg
    17401 taaggacaaa ggcactgaag ctgtagttct taaagctctt ccaaaagtaa acaaagttgt
    17461 tgttgaaggc gtagccctca tcaagaaaca tcaaaaacca aataacgaaa accctcaagg
    17521 cgctatcgtt gaaaaagaag ctccaatcca tgtgtcaaac gttcaagttc ttgataaaaa
    17581 tggtgtagca ggacgtgttg gttacaaagt tgttgacggc aaaaaagttc gttacaacaa
    17641 aaaatcaggc gaagtgcttg attaatcacg aaggaaagga gaagcataat ggcaaatcgc
    17701 ttaaaagaaa aatatactaa tgaagttgtt cctgcgttga cagaaaaatt taactattca
    17761 tctgttatgg ctgtgccaaa agttgagaaa atcgttctta acatgggtgt tggtgatgct
    17821 gtatcaaacg ctaaaaatct tgagaaagct gctgctgagt tagcacttat ttcaggtcaa
    17881 aaaccactta ttactaaagc taagaaatca atcgctggct tccgtcttcg tgaaggtgta
    17941 gcgatcggtg caaaagttac cctccgtggt gaacgtatgt acgaattctt ggataaatta
    18001 gtttctgttt cacttccacg tgtacgtgac ttccatggtg ttccaacgaa atcatttgat
    18061 ggacgtggaa actacactct tggagttaaa gaacaattga tcttcccaga aatcaacttt
    18121 gatgacgttg ataaagtacg tggtcttgat atcgttatcg ttactactgc taacactgat
    18181 gaagagtcac gtgaattgct aaaaggcctt ggaatgcctt ttgcaaagta atctaggagg
    18241 taaataaatt ggctaaaaaa tctatgattg ctaagaacaa acgcccagcg aagttctcta
    18301 cgcaagctta cactcgttgt gaaaaatgcg gacgtccaca ctcagtttac cgcaaattcc
    18361 aactttgccg tgtttgcttc cgtgacttgg cttataaagg tcaagtacca ggcgtaacta
    18421 aagcatcttg gtaataaatt aatgatgctt tgtgtttaaa aggtgtcatt gataatggca
    18481 ctgattttta aacgcaaata tattaagacc tagtcttaca acgtccgtaa ggactcatta
    18541 attagcaagt gattaaattc aaactgctaa taagaggaga attaaaaaat ggttatgact
    18601 gacccaattg cagacttttt aactcgtatt cgtaatgcta accaagcaaa acatgaagtg
    18661 ttagaagtac ctgcatcaaa cattaaaaaa gggattgctg atatccttaa acgtgaaggt
    18721 tttgtaaaaa acgttgaagt tattgaagat gacaaacaag gcatcatccg tgtgttcctt
    18781 aaatatggac aaaatggtga acgtgttatc actaacttga aacgtatttc aaaaccaggt
    18841 cttcgtgttt atactaaaca tgaagatatg cctaaagttc ttaacggact tggtattgca
    18901 attgtatcaa cttctgaagg tcttttgact gacaaagaag ctcgccaaaa aaatgttggt
    18961 ggagaagtac ttgcatatat ttggtaaaat tttacctaaa atgctcattg agtcacagta
    19021 taatcctgtg gtctcatgag tccccgtgaa aactagttgc tcaagcagct tgacaattta
    19081 acaggagaaa ataaacatgt cacgtattgg taataaagta attacattgc ctgcaggtgt
    19141 tgaaatcatt aacaaggata atgttgttac tgtaaaagga cctaaaggcg aactcactcg
    19201 tgagtttaat aaaaatattg gaatcactgt tgaagggact gaagtaacag ttactcgtcc
    19261 taacgactca aaagaaatga aaacaattca tggtacaact cgtgcgaact tgaacaacat
    19321 ggtcgttggt gtttctgaag gtttcaaaaa agctcttgaa atgcgcggtg ttggttaccg
    19381 tgcacaactt caaggatcaa aacttgttct ttctgttggt aagtctcacc aagacgaagt
    19441 tgaagctcca gaaggtgtta cttttgaagt gccaactcca acaacaatca acgtaatcgg
    19501 tatcaacaaa gaaagcgttg gacaaactgc tgcttacgtt cgtagcttac gttcaccaga
    19561 accatataaa ggtaaaggta ttcgttacgt tggtgaattc gtacgccgta aagaaggtaa
    19621 aacaggtaaa taataggatt gatagatttt gtagaatctt ttctgcaaat tctctatctt
    19681 ttcattttaa tattgtcatt ttgacataac aatatattta agaggtgaaa attgtgattt
    19741 cgaaaccaga taaaaataaa atccgccaaa aacgccaccg tcgcgttcgc ggtaaactct
    19801 ctggaactgc tgatcgccca cgtttgaaca ttttccgttc taatacaggc atctacgctc
    19861 aagtaattga tgacgtagcg ggtgtaacgc tcgcaagtgc atcaactctt gataaagaag
    19921 tttctaacgg aacaaaaaca gaacaagccg ttgttgtcgg taaacttgtt gctgaacgcg
    19981 cagtagctaa aggtatttct gaagtggtgt ttgaccgcgg tggatatctc tatcacggac
    20041 gtgtaaaagc tttggctgac tcagctcgtg aaaacggatt gaaattctaa tagggaggac
    20101 acttaataat ggcatttaaa gataatgcag tagaacttga agagcgcgtt gttgcgatta
    20161 accgcgttac aaaagttgtt aaaggtggac gtcgtctacg ttttgcagct cttgtagttg
    20221 ttggtgaccg taatggtcgt gttggttttg gtacaggtaa agctcaagaa gtaccagaag
    20281 ctattcgcaa agctgttgaa gctgctaaga aaaacatggt tgaagttcct atggtcggta
    20341 caacaattcc tcacgaagtt cgttcagaat ttggtggagc gaaagtattg ttgaaaccag
    20401 ctgttgaggg tgctggggtt gccgctggtg gtgctgttcg tgccgtcatc gaattagcag
    20461 gtgttgcaga tattacatct aaatcacttg gttcaaacac tccaatcaac attgttcgcg
    20521 caactgttga gggcttgaaa caattaaaac gtgcagaaga agttgctgct ttacgtggca
    20581 tttctgtttc tgacttagca taagaaaggg gattataatg gctcaaatta aaattacttt
    20641 gactaagtct ccaatcggtc gcaaaccaga acaacgtaaa acagttgttg cccttggact
    20701 tggtaaatta aactcttcag ttgttaagga agataatgct gccatccgtg gtatggttaa
    20761 cgctatctct cacttagtaa ctgttgaaga agcttaatta aaagctaaat cgcactgatt
    20821 gttattggtg cgatttaaac aaatcgtata ggcgagtctt tgcggggagt acctttcccc
    20881 tcgtaaaggc gttagcattt tacaaataag gagaaaaaat aatgaaactt catgaattaa
    20941 aacctgcaga aggatcacgt aaagtccgca atcgtgtggg tcgcggtaca tcttcaggta
    21001 atggtaaaac ttctggacgt ggtcaaaaag gacaaaaagc gcgtagtggt ggtggagtac
    21061 gccttggttt tgaaggtgga caaactccat tgttccgtcg tatgccaaaa cgtgggttct
    21121 caaacatcaa tgcaaaagaa tatgctcttg tgaaccttga ccaattaaat gtctttgaag
    21181 atggtactga agtaactcca gttgttctta aagaagctgg aatcgtgcgt gctgaaaaat
    21241 caggcgttaa aattcttggt aatggtgaat taaccaagaa attgtctgtt aaagcagcta
    21301 aattttctaa atctgctgaa gcagctatca ctgctaaagg tggttcaatc gaagtcatct
    21361 aattaatgag aggtaactca ttatgttttt aaaattactt agagatgcac tcaaagtaaa
    21421 gatggtaaga aataaaattt tatttactat ctttatcctt cttgtatttc gtatcggaac
    21481 gcatatcaca gtaccgggca tcaatgttaa gagtttagaa caaatgggtg aacttccatt
    21541 tctaaatatg cttaacttag ttagtggtaa tgcaatgaga aacttttcgg tattctctat
    21601 gggagttagc ccatacatta ctgcgtctat tgttgttcag ctacttcaaa tggatattct
    21661 tccgaagttt gttgaatggg gaaaacaagg agaggtaggt cgtcgtaagc ttaaccaagc
    21721 tacacgttat atttctttgt tccttgcatt cgtacaatca attggtatta ctgctggttt
    21781 taacactcta tcaagtgttg ctttagttaa aactccgaat gtacaaacat atttgcttat
    21841 tggtgcaatc cttacaacag gtagtatggt ggttacttgg ctaggtgaac aaattactga
    21901 taagggattc ggaaatggag tgtctatgat tatctttgcg gggatcattt cttccattcc
    21961 aagtgcaatt acaactatct acgaagattt ctttgtaaat gtacgctctt cagcaattac
    22021 aaattcttac atctttgtcg ggattcttat tgtagcagta ttagcaattg tattttttac
    22081 aacatttatt caacaagctg aatacaaaat tccaatccaa tatacaaaac ttgttcaagg
    22141 agctcctaca agttcatatc ttcctctaaa agtaaatcca gctggtgtta ttcccgttat
    22201 ctttgctagc tcgattacaa caattccaag tacgattatt cctttctttc aaaatggtaa
    22261 agaaattcct tggcttacta aactccaaga attacttaat tatcaaaccc cagtcggtat
    22321 gattatctat gctatcttga ttattctgtt ctctttcttc tatacttttg tacaagtaaa
    22381 ccctgaaaag acagcagaga atcttcaaaa aaattcgtcc tacattccaa gtattcgacc
    22441 aggacgagaa acagaagagt atatgtcatc gctattgaaa aaattagcaa caattggttc
    22501 agtatttcta gcatttattt ccttgttacc aattattgct caacaagcgt tgcatttgtc
    22561 atcaagtatt gcgttaggtg gtacaagctt actcattttg attgcaacag gtattgaagg
    22621 tatgaaacaa cttgagggct atcttttgaa gagacgctat gttggattca tgaatacaac
    22681 agaatagaat ataggttgac tctgtcaacc ttctattttg ttttttaata agtccgacat
    22741 gctgtgttgg atttatttaa aaacaaaata aggagactat aatgaatctt ttaattatgg
    22801 gtttgcctgg tgctggtaaa ggtactcaag cagctaagat tgttgaagaa tttggtgttg
    22861 ctcacatctc aacaggggat atgttccgcg ccgcaatggc taatcaaacc caaatgggac
    22921 gtttagctaa aagttatatt gataaaggtg aattggttcc tgatgaagta acaaacggga
    22981 ttgtaaaaga gcgcttagct gaggatgata tcgcagaaaa aggtttttta cttgatggat
    23041 atccacgtac tattgagcaa gcacacgcct tagatgctac gcttgaagaa ctaggactac
    23101 gcttagatgg tgttattaat attaaagtgg atccaacatg ccttatagag cgtttgagtg
    23161 gccgtattat caatcgtaaa actggtgaaa ctttccacaa agtgttcaac ccaccagtag
    23221 attataaaga agaagattac tatcaacgtg aagatgataa gcctgaaact gtcaaacgtc
    23281 gcttggacgt taatattgct caaggagaac ctattcttga acactatcgt aagcttggtc
    23341 ttgttacaga tattgaaggt aatcaagaaa taacagaagt ttttgcagat gttgaaaaag
    23401 cgttgctaga actcaaataa attatgctaa tctacttgca taagtagatt agtaatgata
    23461 tactaagtta gtctgactta taattgttac ctctttatag aggacatcaa atcgaaattt
    23521 agggggtact tttgcgtggc aaaagaagat gtgattgaaa ttgaaggtaa agttgttgaa
    23581 actatgccta acgcaatgtt tactgttgag ttggaaaatg gacaccaaat tttggcaact
    23641 gtatcaggaa aaatccgtaa aaactacatc cgtattttgg taggtgatcg tgttactgtt
    23701 gaaatgagcc catacgattt aacacgtgga cgcatcacat accgctttaa atagtcgaaa
    23761 taattggagg gattaaaaca tgaaggtaag accatcggtt aaaccaattt gcgaatactg
    23821 taaagttatt cgtcgtaacg gtcgtgttat ggtaatttgt ccgacaaatc caaaacacaa
    23881 acaacgtcaa ggataatata gaaaggagat aaaatggctc gtattgctgg agttgatatt
    23941 ccaaatgaca aacgtgtagt aatttcactt acttatgtat acggaattgg tctttctaca
    24001 tctaaaaaaa tcttagcagc tgcaggtatt tctgaagata tccgcgttaa agatttgaca
    24061 ccagatcaag aggacgctat ccgtcgtgaa gttgatgcaa tcaaagttga aggtgaccta
    24121 cgtcgtgagg taaacttgaa tattaaacgt ttgatggaaa tcggttcata ccgtggaatc
    24181 cgtcaccgtc gtggacttcc tgtccgtgga caaaatacta aaaataacgc tcgcactcgt
    24241 aaaggtaaag ctgttgcgat tgcaggtaag aaaaaataaa ataggaggta aaacaattgg
    24301 ctaaaccaac acgcaaacgt cgtgtgaaaa agaacatcga atctggtgtt gctcatattc
    24361 acgctacatt caataacact attgttatga ttacagatgt gcatggtaac gctcttgcat
    24421 ggtcatcagc tggtgctctt ggatttaaag gttctcgtaa atctactcca tttgctgctc
    24481 aaatggctgc tgaagctgct gctaaatctg cacaagaaca cggtcttaaa actgttgaag
    24541 ttactgtaaa aggtcctggt tcaggtcgtg aatctgctat ccgtgccctt gctgcagctg
    24601 gtcttgaagt aactgcaatt cgtgatgtga ctcctgtacc acataatggt gctcgtcctc
    24661 caaaacgtcg tcgtgtataa tcatagcatt ttatagtaca caacttttcg tttaagaggg
    24721 agtaactaaa tgattgagtt tgaaaaacca ataataacaa aaattgatga aaataaagat
    24781 tacggcagat ttgtcatcga accactagaa cgtggctacg gtacaactct aggaaactct
    24841 cttcgtcgtg tgcttctgtc ttcacttcca ggtgcagcag tcacatcaat caaaattgat
    24901 ggagtattac acgaatttga tactatccca ggtgtacgtg aagatgtgat gcaaatcatc
    24961 cttaatgtaa aaggacttgc tgtgaaatct tacgtcgaag acgaaaagat tatcgaacta
    25021 gacgttgaag gtcctgcaga aattactgcc ggagatattt taacagatag tgatatcgaa
    25081 attgtaaacc ctgaccatta tcttttcaca attgctgaag gtcacagttt gaaagcaaca
    25141 atgaccgttg ctaaaaaccg aggttatgtt ccagcagaag gaaataaaaa agatgatgca
    25201 ccagtaggaa cattggctgt agattcaatc tatacaccag tgaaaaaagt taattatcaa
    25261 gttgaacctg ctcgtgtagg tagtaatgat ggctttgata aattaactat tgaaatcatg
    25321 acaaatggca caatcattcc tgaggatgct cttggtcttt cagctcgcgt cttaattgaa
    25381 cacttaaact tgtttactga tttaacagag gttgctaaag caactgaagt aatgaaggaa
    25441 acagagaaag tgaacgatga gaaagtgctt gaccgcacaa ttgaggaact tgatttgtca
    25501 gtgcgctcat ataactgttt aaaacgtgct ggaattaata cagtatttga tttaacagaa
    25561 aaaactgagc ctgaaatgat gaaagtccga aacttaggac gtaagagtct tgaagaagtt
    25621 aaaatcaaac ttgccgatct aggtcttgga ttaaaaaacg ataaataaaa taggaggaca
    25681 taatggctta ccgtaaacta ggacgcacta gctcacaacg taaggcaatg cttcgtgatt
    25741 tgacaaccga tcttcttatc aacgaatcaa ttgtaactac tgaagcacgt gctaaagaaa
    25801 tccgtaaaac agttgaaaaa atgattactc taggtaaacg tggtgattta catgctcgtc
    25861 gtcaagctgc tgcttatgta cgtaatgaaa ttgcatcaga aaactatgat gaagcttctg
    25921 ataaatatac atcaacaaca gctcttcaaa aactttttga cgacatcgca cctcgttatg
    25981 ctgaacgtaa cggaggatac actcgtattc ttaaaacaga accacgccgt ggcgatgctg
    26041 ccccaatggc aattattgaa ttagtataat ttttatcaat tttgttgagt gttatgatga
    26101 tggaatcaat cgattcttag tctagctctg gtctaccgct gagaattatt ctcagcggga
    26161 acactcatca tatattgtta aataggtaac gcttgtttac gaaatgcaaa caattttgtt
    26221 tatatttcgt aagcaggcgt tttatgtatt atatttattg gatattttcg atgagcaaag
    26281 agtttaatag ctagactaca tttctcaata gtggttatat aatttctctt aattgaatat
    26341 agaggaatag ttatgtctat actaaattta taaaat
//
LOCUS       DATRUK010000023        25561 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.023, whole genome shotgun sequence.
ACCESSION   DATRUK010000023 DATRUK010000000
VERSION     DATRUK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25561
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.023"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            106..1398
                     /locus_tag="VBE34_000945"
     CDS             106..1398
                     /locus_tag="VBE34_000945"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEO7263673.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDNKKFKLHLIPSGIFFKEKISVIGNGVVVNPKSLVKELAYLHGEGVTTDNLRISDRA
                     HVILPYHIKLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDREVFAERL
                     KINLAEKNRLFEKMYDSTPLEFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGDRIREIGKEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWSEDITACRSLDDLPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWSNI"
     gene            complement(1421..1714)
                     /locus_tag="VBE34_000946"
     CDS             complement(1421..1714)
                     /locus_tag="VBE34_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263674.1"
                     /translation="MILINLIALPICFLAELSAGLLLKLEMHKQHGYYPLERLLMTGS
                     ILTVVSLVLLHTLIHNINSLLFLSIIVVLLISGKSLSLISIYLLKRKSKHQKV"
     gene            2053..2670
                     /locus_tag="VBE34_000947"
     CDS             2053..2670
                     /locus_tag="VBE34_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263675.1"
                     /translation="MRKFFQDVIVEVAYYLEIALSVILATAIIFFSLALFHDLFSMLS
                     VDQNVDKLFQTFLARAMTIAVGIELIKMFSKPSPDTLIEVLLFALARQLVIEHGSVSD
                     YLMGIASVAILFAVRKYLFTHFDESSNIVLRANNKVKKANILARVQLPCQPNETLGDL
                     LVRHLNDEEKDVAIGSVVYINNVALRVDSMHDGLVTRVEIIRSLH"
     gene            2838..4277
                     /locus_tag="VBE34_000948"
     CDS             2838..4277
                     /locus_tag="VBE34_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7263676.1"
                     /translation="MENFLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPLHDVFAG
                     FIKATVGYLILNVGAGGLVNTFRPILVALAKKFHLEAAVIDPYFGLASANAKLETMGF
                     ISVATTALLIGFGINILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQLRNG
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGGILAVLGPDIMSNVKLIGPGAFVPTKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVTELTNAFQGISNKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVVFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLAGGYHGNIDFEFPWLAFGYIFKYLGIAGYVIV
                     CLFFLAIPQLQFMKSKDKEAYYRGDAPAE"
     gene            4305..4583
                     /locus_tag="VBE34_000949"
     CDS             4305..4583
                     /locus_tag="VBE34_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7263677.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVSNFESASCSVGEA
                     KGLAANYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEIL"
     gene            4650..5135
                     /locus_tag="VBE34_000950"
     CDS             4650..5135
                     /locus_tag="VBE34_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7263678.1"
                     /translation="MNLKQAFIENDSIRLKLSASDWKEAIKLSIDPLIESGAVDAEYY
                     DAIIESTEEFGPYYILMPGMAMPHARPEAGVKRDAFSLITLTEPVVFPDGKEVSVLLA
                     LAATSSAIHTSVAIPQIIALFELENSIQRLTECQEAKEVLAMVEESKNSPYLEGLDLE
                     S"
     gene            5248..5913
                     /locus_tag="VBE34_000951"
     CDS             5248..5913
                     /locus_tag="VBE34_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEO7263679.1"
                     /translation="MTKRLPNLQVALDHSDLQGAIKAAVSVGHEVDVIEAGTVCLLQV
                     GSELVEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKEERGDRGEIQIELYGDWTYEQAQQWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLSTDTLQLFEGVDVFTFIAGRGITEADDPAAAARAFKDEIKR
                     IWG"
     gene            5917..6780
                     /locus_tag="VBE34_000952"
     CDS             5917..6780
                     /locus_tag="VBE34_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEO7263680.1"
                     /translation="MTRPIGIYEKATPKHFNWLERLQFAKELGFDFVELSIDESDERL
                     ARLEWSKEERLELVKAIFETGVRVPTITFSGHRRFPMGSNDPEKEARAMDMMKKCIVF
                     AQDIGIRNIQLAGYDVYYEEKSPETRARFIKNLRQACTWAEEAQVILSIEIMDDPFMN
                     SIEKYLAVEKEIDSPYLFVYPDTGNVSAWHNDLWSEFYNGHRSIAALHIKDTYAVTET
                     SKGQFRDVPFGQGCVDWEEMFAVIKKTNYNGPFLIEMWSENCETVEETRAAIKEAQDF
                     LYPLMEKAGVK"
     gene            6782..7498
                     /locus_tag="VBE34_000953"
     CDS             6782..7498
                     /locus_tag="VBE34_000953"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEO7263681.1"
                     /translation="MAKSLQEMRERVCEANKSLPVHGLVKFTWGNVSEVDREAGLIVI
                     KPSGVDYDQLTPENMVVTDLEGNIVEGDLNPSSDLPTHVQLYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLSEDEVNTAYEKETGSVIIEEFERRNL
                     DPMAVPGIVVRNHGPFTWGKDPAQAVYHSVVLEEVAKMNRFTEQINPRVEPAPKYIMD
                     KHYLRKHGPNAYYGQKGQEH"
     gene            7509..8156
                     /gene="fsa"
                     /locus_tag="VBE34_000954"
     CDS             7509..8156
                     /gene="fsa"
                     /locus_tag="VBE34_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO7263682.1"
                     /translation="MKYFLDTADVSEIRRLNRLGIVDGVTTNPTIISREGRDFKEVIN
                     EICQIVDGPVSAEVTGLTCDEMVTEAREIAKWSPNVVVKIPMTEEGLAAVSQLSKEGI
                     KTNVTLIFTVAQGLSAMKAGATFISPFVGRLEDIGTDAYALIRDLRHIIDFYGFQSEI
                     IAASIRGLAHVEGVAKCGAHIATIPDKTFASLFTHPLTDKGIETFLKDWDSFKKK"
     gene            8319..9302
                     /locus_tag="VBE34_000955"
     CDS             8319..9302
                     /locus_tag="VBE34_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7263683.1"
                     /translation="MFEKVTISDIANLVGVSKATVSYYLNGNYKKMSLQTKEKIRLAI
                     KETGYQPSKIAQSLVTKNTRTIGVVIADITNPFISSVMKGIHDTCQQFGYSVNFTNSD
                     NDIDIELENLNRLNQQNVSGIILDSVDPNHSFIETLSNDRLVMVDRQAKDIKVDTVVS
                     DNKESTQIFLEKMQETGYHDIYFVTYPIEGISTRELRYEGFKEVVSSNPDKLIIITED
                     GSTQRILDIIEHSEQKPGFLMMNGPTLLNFMKKLNQSTVRYPEDYGLGSYEDLEWMQV
                     LTPNVSCIKQDSYGIGCLAAQCLIEKISQGNEPTTARLLEVKNQIVIRQSF"
     gene            9537..10556
                     /locus_tag="VBE34_000956"
     CDS             9537..10556
                     /locus_tag="VBE34_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263684.1"
                     /translation="MKINKLLSDRLEACYSVCRLQNQDYDFLLVASEVDQACFAYDLN
                     SDLAKIVVWEDVGGTMSIIPIPGTMDFLATQKFYPGFKAKDCQVVYASFQGDHWDICN
                     AFDFPYLHRFDLIKGKENQLYFIGCTIANSKAYTEDWSDKGRIFVARFNTQNHTLEGL
                     QQLPQTLLKNHGYYAIQDEGYSLITSVEGVLKLTYPEFSTTGDWQLERLFDEETSDVV
                     KVDINQDGKDEYVIIQGFHGDRLRIFTEDFGRELFHYPEKTPFGHAIWSGRLLNQTCF
                     VFGWRSEKAELRLFHFVDGHLVSELVDAKAASSNVLAFEKDGKAYLFSANNGRGEVAL
                     YQLVK"
     gene            10575..11531
                     /locus_tag="VBE34_000957"
     CDS             10575..11531
                     /locus_tag="VBE34_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO7263685.1"
                     /translation="MDKKKILVTGIVPKEGLRKLMDRFDVTYSEDRPFSRDYVLEHLS
                     EYDGWLLMGQKGDKEMIDAGENLQIISLNAVGFDHVDTAYAKEKGIVVSNSPQAVRVP
                     TAEMTFALILAASKRLAFYDSIVRSGEWIDPSEQRYQGLTLQGSTLGIYGMGRIGLTV
                     ANFAKAFGMTVVYNDVYRLPEDKEKELGVTYLEFDQLIETADVITIHAPALPSTIHKF
                     NKDVFAKMKNRSYLINAARGPIVSEEALIEALKEGEIAGAGLDVFENEPQVSEGLRSL
                     DNVIMSPHAGTGTIEGRRTLAEEAADNIIAFFDGKPQNIVNK"
     gene            11629..13080
                     /locus_tag="VBE34_000958"
     CDS             11629..13080
                     /locus_tag="VBE34_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO7263686.1"
                     /translation="MLEALHKFFEVFGAATVVPAMIFIVSLFLKVNPRRAFFSALRAG
                     VGLTGFGWIISAFAPMVTKYIQQMVKTTGLHLPIVDIGWQAGSLTAFSSEIGLSFFVF
                     GLLIELGLFLLGITRVFVPSNLWNNFGYMIWGTMAYAATGNFILSFAFMVFVLLYSLV
                     MSEVLADRWSEYYGVKNATINSIHNIETLIPALILDPLWNLLGVNKVKLNPESLKTKL
                     GIFGEPMTLGFILGVIIGVLGSLRNLASIDTWGGILGFAVALAAVMTIFPLITGVFAS
                     AFAPLAEAVERNKKKESQAEQGALDKKRWFIAVDDGVGFGEPATIIAGLILVPIMVVI
                     SLILPGNEALPVVDLIAIPFMIEAMIAVSKGNILKAILNGIIWFSLGLYAASALGPIY
                     TEAVKHFGTALPAGVTLIMSFNLLAHPLTALVFFAWISGNPLYIGVTVIIYLAFLFLL
                     RTRRESIYAYLKTMADKNSGYEGSCEKIFIAED"
     gene            13102..14091
                     /locus_tag="VBE34_000959"
     CDS             13102..14091
                     /locus_tag="VBE34_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO7263687.1"
                     /translation="MKKIDGHAHIVDTIAGFNGKGRLNALGNGYAIWDDGDLIKLIPD
                     EYGDKAFTAESFLKYMDANGVDKAVILQGHLNGYQNYYTHLAIKRYPERFTGAFSVDP
                     FADNAMQIVKRHVEVLGFRAIKFEISQGGGIHGYRGQKTPFRLDTDPHVSRILTYLLD
                     YPGFVVTVDYGNWDQISHQPDAIANLARLYTSLDFVVCHLSFPHVEHSNRLRAELNMW
                     KNFNNIYTDISAIQDIDAPDSFPFPKSEQNVRIAKEVLGAKRIIWGTDSPWSATFNTY
                     EELATWLENVDIFSQEELEDVMYNNAERVYFKEEHVKANRAGIDDTTKELNLY"
     gene            14157..15674
                     /locus_tag="VBE34_000960"
     CDS             14157..15674
                     /locus_tag="VBE34_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEO7263688.1"
                     /translation="MTYYLSIDYGGTNTKALIFDKLGHQIAVSSFETLKNEAQSGHRQ
                     VNLVKTWNAITSAIREVIQISKLSPEQISAVACIGHGKGLYLLDNKLEPLEQGILSTD
                     NRAKDLAQYFESKLDNIWELTRQHIFPSQSPVILRWLKDYQPETYKSIGAVLSAKDFI
                     RYKLTGKVQQEYGDASGNHWINFQTGTYDPAILDFFGIREIENSLPELIDSADLVPGG
                     ISSQAAKETGLVEGTPVVGGLFDIDACALGSGVLESDTFSVISGTWNINTYPSLKPAK
                     QDSGLMTSYFPDRRYLLEASSPTSAGNLDFMLKMLMHQEIDNAKSSGGSIYDNLEEFL
                     THTDATHHGLIFFPFLYGSNTSQDASACFFGLTTKSTKSQMIRAVYEGIAFAHKQHIT
                     DLIKSRGGVPKIIRFSGGATNSPAWMQMFSDILNLPIETVEGTELGGLGGAILARHAL
                     DKISLKEAVQDMVRVKAIYKPQLSEVKGYEKKYHAYQKLLETLDPIWSELGHLNK"
     gene            15694..16455
                     /locus_tag="VBE34_000961"
     CDS             15694..16455
                     /locus_tag="VBE34_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP endonuclease"
                     /protein_id="HEO7263689.1"
                     /translation="MKQEDIIINSIVFKKQLDKGFTQVQLIKLVKDLGFSRFEIRQEL
                     LQDPDRELPALKAEADFYDINLYYSANEDLIKGGKVNPYLNKGLKEASQLGAPFIKLN
                     VGQTRNLSKEELEPLKEILKSQTIGIKVENNQDPKAATVENCQYFMTLVKELQIPISF
                     VFDTANWAFINQDLYQAVNNLACDTTYLHCKNFIQVAGKPHLSKSLFEGEINLTDLLK
                     SFSNCEYLALEYPTELEILKRDVQRLISISNSQNF"
     gene            16521..18200
                     /locus_tag="VBE34_000962"
     CDS             16521..18200
                     /locus_tag="VBE34_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEO7263690.1"
                     /translation="MIILDKKSYDLLFYLLKLEEPETVMAIANALNQSRRKVYYHLEK
                     INDALPSDVPQIVSYPRVGILLTEKQKAACRLLLDEVTDYSYVMKSSERLQLSLVSIV
                     VAKDRITIDRLMQLNDVSRNTILNDLNELRSELAEKEYNLQLQSTKCRGYFLDGHPLS
                     IIQYLYKLLDDIYHNGSSSFIDLFNHKLSQAFGASTYFSKEVLDYFHHYLFISQRSLG
                     KKINSQDGQFMIQILPFILMAYRKMRLSPEVQTSLNSDFSLVWQRKEYEIAKELADEL
                     EENFQLSLDEIEVGLVAMLMLSFRKDRDNHLESQDYDDMRATLTSFLKELEERYHLHF
                     VHKKDLLRQLLTHCKALLYRKRYGIFSVNPLTEHIKDKYEELFAITSSSVKLLEKAWQ
                     IKLTDDDVAYLTIHLGGELRNSQQSPNKLKLVIVSDEGIAIQKLLLKQCQRYLTNSDI
                     EAVFTTEQYQSVSDLMHVDMVVSTSDALESRFPMLVVHPVLTDDDIIRLIRFSKKGNC
                     ANSNQFTNELEKTIAQYVKEDSERYVLKSKIEKLIHQELLQDVLPLQSTVC"
     gene            18517..19608
                     /gene="ulaG"
                     /locus_tag="VBE34_000963"
     CDS             18517..19608
                     /gene="ulaG"
                     /locus_tag="VBE34_000963"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEO7263691.1"
                     /translation="MPNVKDITRESWILSTFPEWGTWLNEEIEEEVVAEGNFAMWWLG
                     NCGVWIKTPGGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVI
                     DPFAINELDYYLVSHFHSDHIDINTAAAIINNPNLDHVKFVGPYECGEIWKKWGVPEE
                     RIIVIKPGESFEFKDIKVTAVESFDRTCLVTLPVDGAEEHDGELAGLAVTDEEMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDYKIDVAINNYGDNPVGIQDKMTSIDLLR
                     MAENLRAKVIIPVHYDIWSNFMASTDEILQLWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DKDRIEYHHPRGFDDCFEQESNIQFKALL"
     gene            19698..22076
                     /locus_tag="VBE34_000964"
     CDS             19698..22076
                     /locus_tag="VBE34_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEO7263692.1"
                     /translation="MSEFDTKSYLEKLDAWWRAANYISAAQMYLKDNPLLRRELVEND
                     LKVHPIGHWGTVPGQNFIYAHLNRAINKYDLDMFYIEGPGHGGQVMVSNSYLDGSYTE
                     LNPNIEQTEDGFKQLCKIFSFPGGIASHAAPETPGSIHEGGELGYALSHATGAILDNP
                     DVIAATVIGDGEGETGPLMAGWLSNTFINPVNDGAVLPIFYLNGGKIHNPTIFERKTD
                     EELSQFFEGLGWKPIFADVVELSEDHAAAHALFAEKLDQAIQEIKTIQSEARQKPAEE
                     AIQAKFPVLVARIPKGWTGPKAWEGTPIEGGFRAHQVPIPVDAHHMEHVDSLLSWLQS
                     YRPEELFDENGKIVDEIAAISPKGDRRMSMNPITNAGIVKAMDTADWKKFALDINVPG
                     QIMAQDMIEFGKYAADLVDANPDNFRIFGPDETKSNRLQEVFTRTSRQWLGRRKPDYD
                     EALSPAGRVIDSQLSEHQAEGFLEGYVLTGRHGFFASYESFLRVVDSMVTQHFKWLRK
                     SKTHTTWRKNYPALNLIAASTVFQQDHNGYTHQDPGILTHLAEKTPEYIREYLPADTN
                     SLLAVMDKAFKAEDKINLIVTSKHPRPQFYSIAEAEELVAEGYKVIDWASNVSLNQEP
                     DVVFAAAGTEPNLEALAAISILHKAFPELKIRFVNVLDILKLRHPSQDARGLSDEEFN
                     KVFTTDKPVIFAFHGYEDMIRDIFFSRHNHNLHTHGYRENGDITTPFDMRVMSELDRF
                     HLAQDAALASLGNKAQAFSDEMNQMVAYHKDYIREHGDDIPEVQNWKWENIK"
     gene            22453..23676
                     /locus_tag="VBE34_000965"
     CDS             22453..23676
                     /locus_tag="VBE34_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO7263693.1"
                     /translation="MTNILEVKNLTKIFGKKQKAALEMVKQGKSKTEILEKTGATVGV
                     YDASFEIKEGEIFVIMGLSGSGKSTLVRMLNRLIDPSSGNIYLDGKDIAKMNVEDLRN
                     IRRHDINMVFQNFGLFPHRTILENTEFGLEMRGVSKEERTTLAEKALDNAGLLPFKDQ
                     YPSQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDTNKQTI
                     IFISHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTVLEIDGPQVALTRMHREEVSMLMATNRRRQLLGSLTADAAIEARKKGL
                     PLSEVIDKDVVTVSKDTVITDIMPLIYDSSAPIAVTDDNDRLLGVIIRGRVIEALANV
                     QDETVVESPKETVEA"
     gene            23695..25422
                     /locus_tag="VBE34_000966"
     CDS             23695..25422
                     /locus_tag="VBE34_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEO7263694.1"
                     /translation="MENLLQHKLPVAPFVESTTNWITKTFSGLFDFIQTIGNALMDWM
                     TKTLLFINPLLFIVLITIAVFFLAKKKWQLPTFTFIGLLFIYNQGLWEQLINTFNLVL
                     VASLISIIIGVPLGIWMAKSDKVKQVVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVVFALPPTVRFTNLAIREIPLELIEASDSFGSTAKQKLFKVELPLAKNTIMAGIN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGTGFVSGLSLVILAIVLDRVSQFF
                     NSKPGEKQAKTSKVKKWVGLGALALFILAALGRIVVNMTSGNEAKGQKVKIAYVQWDS
                     EVASTNVIAEVLKSKGYDVELTPLDNAVMWQTVANGNADFTTSAWLPKTHGQYFNKYK
                     NSLDDLGPHVENVKIGLVVPKYMNVNSIEELSNQADKQITGIEPGAGIMKSAKQSLKD
                     YPNLSSWKLLSASTGAMTTTLGKAIKNKDQVVITGWSPHWMFAKYDLKYLKDPKKSFG
                     GEEHINTIARKNLKKDMPKVYKIIDKLKWTKEDMESIMLDMDKGMEPAKAAQKWIKNH
                     KKEVSEWTK"
BASE COUNT         8071 a         4192 c         5155 g         8143 t
ORIGIN      
        1 tttgctataa taaggtgtca gacaaactcg caggtgaaat tcctgccatg tttttccaga
       61 aaggattggg gagtcccaaa aaggctcact cagtgttaaa taaatatgac ttcagttgtt
      121 gtagtaggta cccaatgggg tgacgaaggt aaaggtaaaa ttacagattt tttatctgct
      181 gacgcagaag taattgcacg ttatcaaggt ggtgataacg ctgggcatac tatcgttatt
      241 gataacaaaa aatttaaact tcatttaatt ccttcaggaa ttttcttcaa agaaaaaatt
      301 tctgtcattg gtaatggtgt tgtcgttaat ccgaaatcat tagttaaaga gttagcttat
      361 cttcacggag aaggtgtaac aacggataac cttcgtattt cagatcgtgc gcatgttatc
      421 ttgccatacc acatcaagct tgaccaacta caagaagatg ctaaaggtga taataaaatt
      481 ggaacaacca tcaaaggaat tgggccagct tacatggaca aggctgcgcg tgtcggtatc
      541 cgtattgctg accttcttga tagagaagtt tttgctgagc gtttgaaaat taatttagct
      601 gagaaaaatc gtttatttga aaaaatgtat gacagtacac ctcttgaatt tgatgatatt
      661 ttcgaagaat attatgaata tggtcaacaa attaagcaat atgtgactga tacatccgtt
      721 atcttaaatg atgctcttga tgccggtaaa cgtgttcttt ttgaaggagc acaaggagtt
      781 atgctcgaca ttgatcaagg aacataccca tttgtaacat cttccaatcc agtagcaggt
      841 ggtgtcacaa ttggttcggg agttggacca agtaaaatta ataaagtggt aggtgtatgt
      901 aaagcttaca ctagccgtgt tggcgatgga ccattcccaa cagaactttt tgatgaggtt
      961 ggtgaccgta ttcgtgagat tggtaaagag tatggtacaa cgactggtcg tcctcgtcgc
     1021 gttggatggt ttgattctgt tgttatgcgt cacagccgtc gagtatcagg tattactaac
     1081 ctctctctga attcaattga tgttctttca gggcttgata cggtgaaaat ttgtgtggct
     1141 tatgaccttg atgggaaacg tattgactat tacccagcaa gccttgaaca gctaaaacgt
     1201 tgtaaaccaa tctatgaaga attaccgggc tggtctgaag atattacagc ttgccgtagc
     1261 ttagatgatc ttccagaaaa tgcacgtaat tacgttcgcc gtgttggcga attggttggt
     1321 gttcgtattt ctactttctc agtagggcct ggtcgtgaac aaacaaacat tcttgagtct
     1381 gtttggtcaa atatttaata ttaaaaatct gactgtacag tcagactttt tgatgtttac
     1441 tttttctttt taatagatag atactaataa gacttaagga cttcccagaa atcaataaaa
     1501 ctactataat tgataaaaaa agaagtgaat tgatattgtg aatcaaagta tgtaataata
     1561 ctagcgatac aactgttaga atactaccag tcatcaataa gcgttccaga ggatagtagc
     1621 catgttgctt atgcatttct aattttaata gtagaccagc tgacaattct gctaggaagc
     1681 aaataggtag agctatcaaa ttaataagaa tcatagacca accaaatcct tgaccataac
     1741 ctatatatga tagactattt ttgggaattg ttgacgtgtg aattataaaa tcaatcgtaa
     1801 agataatgtc tataagaagt agtagcgcta acagataatg tagtaagaag attgttctta
     1861 accgtttatt aatcaatatt tcatttcctc ctacttattt accaataagc ttattatatc
     1921 ataatgtata atcaaacatt cgttagtact gattaaaagg tttatggagg ggcggttact
     1981 attgtttcat tgaattagta actttaacga cgaaatgtta taatgttatt agtttataaa
     2041 agaggagatt gtatgcgtaa atttttccaa gatgttattg tagaggtggc ttattattta
     2101 gagattgctt tgtcagttat tctggcaaca gctattattt tctttagttt agcactcttt
     2161 catgatttgt ttagcatgtt gtctgttgat caaaatgttg ataagttgtt ccagactttc
     2221 cttgctcgcg ccatgacaat agcagtcggg attgagttga tcaaaatgtt ttcaaaacct
     2281 tcccctgata ctttaattga agtgttattg tttgccttag ctcgtcaact tgtaattgaa
     2341 catggtagtg tttcagatta ccttatgggg attgcttcag ttgctatttt gtttgctgtt
     2401 cgtaaatatc ttttcaccca ttttgatgag tcaagcaaca ttgttttacg tgctaataac
     2461 aaagtcaaaa aagctaatat tttagctagg gttcaactac cttgtcaacc caatgaaact
     2521 ttaggagact tacttgttcg tcatttaaat gatgaggaaa aggacgttgc tattggttca
     2581 gtagtctata ttaataacgt tgcattgcgt gttgatagta tgcacgatgg tcttgtgacg
     2641 cgtgtagaaa tcattaggag tctacattaa aaaatataaa aaatcatgta tcttaggata
     2701 catgattttt ttgtaaatta ggttcagcac aattttgcac acttaagtgt gtacttcttg
     2761 gtctttttta aaacgcttac aaaaaatata ataaagatac aaaaaaacat tacttaaata
     2821 aagagaggtc tttagttatg gaaaacttcc tagctcccct taattggttc tcgcaaaata
     2881 tcttgcagaa tccagccttc ttcgtaggtc tgttggtact cattgggtat cttttattga
     2941 agaaaccact tcatgatgtc tttgctggtt ttatcaaagc gaccgttgga tatttgattt
     3001 tgaacgtagg tgcaggagga ttggttaata cattccgtcc aatcttagtt gctcttgcta
     3061 aaaaatttca tttagaagct gctgttattg acccttactt tggtttagca tcagcaaatg
     3121 ctaagttaga aacaatgggc tttatcagtg ttgcaacaac agcactcttg attggttttg
     3181 ggattaacat tctcttggtt gctcttcgta aagtaactaa agtccgtact ctttttatta
     3241 ctggtcatat catggtacaa caagctgcaa caatttcagt atttgtactg ctcttgattc
     3301 cacaacttcg taatggtttt ggtgcatggg ctgttggtat catttgtggt ctatattggg
     3361 cggtaagttc taatatgacc gttgaagcaa ctcaacgcct tacaggtggt ggcggatttg
     3421 ccatcggaca tcaacaacaa tttgcaattt ggtttgttga taaagttgcg ccatttttcg
     3481 gtaaaaaaga agaaaatctt gataacttaa aattaccaac attcttaaat attttccatg
     3541 atacagttgt tgcatcagca acattgatgc tagttttctt tggaggaatc ttagctgtcc
     3601 ttggtccaga cattatgtct aatgttaaat taatcggacc tggtgcattt gtaccaacta
     3661 agcaagcttt cttcatgtac atccttcaaa catcattgac attctcagtt taccttttca
     3721 ttttgatgca aggtgtacgc atgttcgtta cagaattgac aaatgccttc caaggtatct
     3781 caaacaaact tcttccagga tcattcccag cggttgacgt tgcagcttca tatggttttg
     3841 gttcttcaaa tgcagttctt tcaggatttg cttttggttt gattggtcaa ttaattacaa
     3901 tcgctctact tgttgtcttc aaaaatccaa tcttgattat cacaggattc gttccagtat
     3961 tctttgataa tgcggcaatc gctgtttatg cagataaacg tggtggctgg aaagcagcag
     4021 tagctctatc atttatctca ggtattattc aggtggcact tggtgctgtt gctgtaggtc
     4081 ttttaggact tgcaggtggt tatcatggaa atatcgactt tgaattccca tggttagcat
     4141 ttggttacat cttcaagtat ttaggtattg ctggttacgt gattgtatgt ctcttcttct
     4201 tagctattcc acaacttcaa tttatgaaat ctaaagataa agaagcatac tatcgtggtg
     4261 atgcgcctgc agaataatag aaatttataa cggaggaaat aaaaatggtt aaagttctta
     4321 cagcatgcgg aaacggcatg ggatcatcaa tggttatcaa aatgaaagtt gaaaatgcgc
     4381 ttcgtcaatt aggcgttagc aactttgagt cagcatcttg ctcagtcgga gaagcaaaag
     4441 gtttagctgc gaactatgat atcgtggttg cttcaaacca ccttatccat gaattagacg
     4501 gacgtacaaa aggtcattta gtaggtcttg acaaccttat ggatgacaat gaaataaaaa
     4561 caaaactaca agaaattttg taagttaata gacaaaggga gtgagacaaa agtttctctc
     4621 ccatatcttt agacttttaa ggagaaatca tgaatttaaa acaagccttt atcgaaaatg
     4681 actcaattcg tcttaaatta tctgcaagcg attggaaaga agcaatcaaa ttatcaattg
     4741 atccattaat tgaaagtggt gctgttgatg cagaatatta cgatgccatt attgaatcta
     4801 cagaggaatt tggtccgtac tacattctta tgccgggtat ggcgatgcct catgctcgtc
     4861 cagaagcagg cgtaaaacgt gatgcatttt cattaattac attaacagag ccagtagtct
     4921 ttccagatgg caaagaagtc tcagtattgt tagcacttgc tgcaacaagt tcagctattc
     4981 atacttctgt tgctatccct caaattattg ccctttttga attggaaaat tcaattcagc
     5041 gcttgacgga atgccaagaa gcaaaagaag tccttgcaat ggtagaggaa tcaaaaaaca
     5101 gcccatattt agaaggttta gatttagaaa gctaatcaat aaactgatat taatagaaag
     5161 gataggactt ataaaaaatc tctgtgataa gaaactcttt gccacaaata aagtgagtat
     5221 ttttatcagg tccttagaat aaacaacatg acaaaacgtt taccaaattt acaagttgca
     5281 cttgaccact cagatttaca aggagctatc aaagctgccg tatcagtagg acatgaagtt
     5341 gatgttattg aagctggaac tgtttgcctt cttcaagtag gtagtgaatt agttgaggtc
     5401 cttcgcagtc tcttccctga caaaataatt gttgctgata caaaatgtgc tgatgctggt
     5461 gggactgttg ctaaaaataa tgctgttcgc ggtgcagact ggatgacttg tatctgttgt
     5521 gctactattc caacaatgga agcagcctta aaagctatca aagaagaacg cggcgatcgt
     5581 ggagaaattc aaatcgaact atatggtgat tggacttatg agcaagcgca acaatggttg
     5641 gatgcaggta tttcacaagc catttatcac caatctcgtg atgcgcttct tgctggcgaa
     5701 acatggggag aaaaagatct taataaggtt aaaaaattga ttgatatggg cttccgagtt
     5761 tctgtaactg gtggtttatc aactgatact cttcaattat ttgaaggtgt tgatgtcttt
     5821 acttttattg cgggtcgtgg tattacagag gcagatgatc cagccgcagc agcacgtgca
     5881 tttaaggatg aaattaaacg tatttggggg taatgtatga cacgtccaat aggaatttac
     5941 gagaaggcaa caccaaaaca ttttaactgg ttagaacgcc tacaatttgc taaagaattg
     6001 ggatttgatt ttgtagaatt atccattgat gagtcagatg aacgtttggc acgtttggag
     6061 tggtcaaaag aagaacgcct tgagttggtc aaagctattt ttgagacagg agttcgtgtt
     6121 ccaacaatta catttagtgg tcatcgtcgt ttcccaatgg gatctaatga tcctgagaaa
     6181 gaagcgcgtg cgatggatat gatgaaaaaa tgtatcgtct ttgctcaaga tattggtatt
     6241 cgtaatattc aattagcagg atatgatgtg tactatgaag aaaaatctcc agagacacgt
     6301 gcacgcttta ttaagaatct tcgtcaagct tgtacttggg ccgaagaagc acaagttatt
     6361 ctgtctattg agattatgga tgacccattt atgaattcca ttgagaaata tttggctgtg
     6421 gaaaaagaga ttgattctcc ttatctcttt gtctacccag atactggaaa tgtatcagca
     6481 tggcataatg atctttggag tgaattttat aatggacatc gctcaattgc agcgcttcat
     6541 attaaagata cctatgctgt taccgaaacg tctaaaggtc aattccgtga tgttccattt
     6601 ggtcaagggt gtgttgattg ggaagaaatg tttgctgtca ttaagaaaac aaattataat
     6661 ggtccattct tgattgaaat gtggtcggag aactgtgaga ctgtagagga aacacgtgca
     6721 gccattaaag aggcgcaaga ttttctttac ccattaatgg agaaagcagg agtgaaataa
     6781 tatggcaaaa tcattgcaag aaatgcgtga acgcgtgtgt gaagctaata aatcactacc
     6841 agttcatggt ttagttaaat ttacttgggg taacgtttct gaagttgacc gcgaagcagg
     6901 attgattgtt atcaaacctt caggagttga ctatgaccaa ttgacacctg aaaatatggt
     6961 tgtcacagac ttagagggaa atatagtgga gggggatttg aatccatcat ctgatcttcc
     7021 aactcatgtt caactttata aagcatggcc agaagtcggt ggtattgttc atactcattc
     7081 aacagaagca gttggctggg cacaagcggg tcgcgatatt ccattttatg gaacaacaca
     7141 tgcagattat ttttatgggc cagtaccatg tgctaggtca cttagtgaag atgaagttaa
     7201 cactgcttat gaaaaagaaa caggtagtgt tattatagag gaatttgaac gtcggaattt
     7261 agatccaatg gctgttcctg gtattgttgt acgtaatcat ggaccattta cttgggggaa
     7321 agatccagca caagcagttt atcactcagt cgttcttgaa gaagttgcca agatgaatcg
     7381 tttcactgaa caaattaatc cacgtgttga accagctcca aaatatatta tggataaaca
     7441 ctatttacgt aagcatggac caaatgctta ttatggtcaa aaaggacaag aacattaagg
     7501 agaataatat gaaatacttt ttagatactg ccgatgtttc tgaaattcgt cgtcttaatc
     7561 gtttaggaat cgttgatggt gttactacca atcctactat tatttcacgt gaggggcgtg
     7621 actttaaaga agttattaac gaaatttgcc aaatcgttga tggtccagtt agtgcagaag
     7681 tgacaggttt aacttgtgat gaaatggtta ccgaagctag agaaatcgct aagtggtcac
     7741 caaatgttgt tgttaaaatt ccaatgactg aagaaggttt agcagcagtt tcacaattat
     7801 caaaagaagg tataaagaca aatgtgacat taatatttac tgtggctcaa gggctttcgg
     7861 caatgaaagc tggtgcaacc tttattagtc cttttgtggg tcgtcttgaa gatattggaa
     7921 cagatgctta tgctttgatt cgtgaccttc gtcacattat tgatttttat ggtttccaat
     7981 ctgagattat tgctgctagc attcgtggat tggcgcatgt ggagggcgtc gctaaatgcg
     8041 gtgcgcacat tgccactatt ccagataaaa catttgcatc actatttact cacccgctta
     8101 ctgataaagg gattgaaact ttcttaaaag attgggatag tttcaagaag aagtagtgtt
     8161 acgataaagc aaactcttaa ttatactagt gaaagacaaa ctttgataat gggtttgtct
     8221 tttttataat cttctccaat aagaacactt attttcattt ttcttattaa atgatataat
     8281 acggataaat taagtctaat tagaataagg aaatagagat gtttgagaag gtaaccatta
     8341 gtgatattgc aaatttagta ggtgtgtcaa aagctacagt ttcctattat ctcaatggga
     8401 actataagaa aatgtctctg caaacgaaag aaaaaatccg tttagctatt aaggagactg
     8461 gttatcaacc aagtaagatt gctcaaagtt tagttacaaa aaatacccgt actattgggg
     8521 tcgttattgc tgatattacc aatccattta tttcgtctgt gatgaaggga atacatgata
     8581 cttgtcaaca atttggttac tcggttaact ttactaactc ggataatgat attgacattg
     8641 agttggaaaa tttaaaccgt ttaaatcagc aaaacgtttc aggaattatt ttagattctg
     8701 tagatcctaa tcattcattt atagaaactt tgagtaatga tcgtcttgtc atggttgata
     8761 gacaagctaa agatattaag gtagatactg tagtatccga caataaagaa tcgactcaaa
     8821 tatttcttga aaaaatgcaa gaaactgggt atcatgacat ctattttgtc acttatccca
     8881 ttgaagggat ttcaacccgt gagcttcgtt acgaaggttt taaagaagtg gtcagttcaa
     8941 atcctgataa gttgattatt atcactgaag atggtagcac acagagaatt cttgatatta
     9001 ttgaacattc agagcaaaaa ccagggttct taatgatgaa cggtccaact cttttgaatt
     9061 ttatgaaaaa attaaatcaa tcaacggtca gataccctga ggattatggt cttgggagtt
     9121 acgaagattt agaatggatg caggtgctta caccaaacgt ttcctgtata aaacaggatt
     9181 catatggcat tggttgtctg gctgcccaat gtttaattga aaaaattagt caaggaaacg
     9241 aaccaactac tgcaaggtta ctagaagtaa aaaatcaaat cgttattcgt caatcttttt
     9301 aaaaatgttt gcgagagcaa gcattttttt ataaaaaagc aaaaaacact atgtaaacgc
     9361 ttgcttttat aaaaaatgat agtataatag aactataaat gagttaaccg tttaacttaa
     9421 gaataggtaa gcttttaacg aaccatctct aactctctta actcaaatgt ttttattttt
     9481 ttgcaccatt agttaaacgg ttaactcaag ttttatcttc accaaaggag gatagaatga
     9541 agattaataa attattgtca gacagacttg aagcttgtta ttcagtttgt cgtttgcaaa
     9601 atcaagacta tgatttttta ttagtagctt cagaggttga tcaagcctgc tttgcttatg
     9661 atttgaatag cgatttagca aaaattgttg tttgggaaga tgtgggaggt accatgagta
     9721 ttattccaat tcccggaacc atggattttt tggcaaccca gaaattttac ccagggttta
     9781 aggccaaaga ttgtcaggtt gtttatgcaa gttttcaagg agatcattgg gacatttgta
     9841 acgcatttga ttttccgtat ttacatcgct ttgatctcat taaaggtaaa gaaaatcaac
     9901 tttactttat aggttgtaca attgctaaca gtaaagccta cactgaggat tggagtgata
     9961 aaggccgaat ttttgttgct cgttttaata ctcaaaacca tacattggaa ggattgcaac
    10021 aattgcctca aactttatta aaaaatcatg gatactatgc cattcaggat gaaggatatt
    10081 cattgattac ttcagtagaa ggggtactca aactcactta tccagaattt tctactacag
    10141 gcgactggca attagaacgg cttttcgatg aggagacaag cgatgtggtg aaagtggata
    10201 ttaatcagga tggtaaggat gagtatgtga tcatccaagg ttttcatgga gatcgtttac
    10261 gtatcttcac tgaagatttc ggtcgagaat tattccatta tcctgaaaaa accccatttg
    10321 gtcacgctat ttggagtggt cgtttactta atcagacttg tttcgtattc gggtggcgat
    10381 cagaaaaagc agaattaagg ctttttcact ttgtagatgg gcacttggtt tcagaattag
    10441 tagatgcaaa agcagcttct agtaatgtct tagcttttga aaaagatgga aaagcttatc
    10501 ttttctcagc caataacgga cgtggcgaag ttgctcttta tcaattagta aaataaattt
    10561 aagaaggaga caatatggac aagaaaaaaa tcttagtaac gggtattgtg cctaaagaag
    10621 gtctaagaaa gcttatggac cgatttgatg ttacttattc agaagatcgc ccattttcac
    10681 gtgactatgt gttagagcat ttatctgaat atgacggatg gttactcatg ggacaaaaag
    10741 gtgataaaga gatgattgat gcaggtgaaa acttacaaat tatttcttta aatgcggttg
    10801 gttttgatca tgtagatact gcgtatgcta aagaaaaagg aattgtagtc tctaattccc
    10861 ctcaagcagt acgagttccg acagcggaaa tgacttttgc tttaatttta gctgccagta
    10921 agcgccttgc attttatgat agtattgtaa gaagtggaga gtggattgat ccgagcgaac
    10981 aacgttatca aggtttgact ttgcaaggtt caacattagg tatttacgga atgggacgca
    11041 tcggtttgac agtcgcaaat tttgctaaag catttgggat gactgttgtt tacaatgatg
    11101 tttatcgact tcctgaagat aaggaaaaag agcttggtgt aacctatctc gaatttgatc
    11161 agcttattga aacagctgat gtcataacca tccacgcccc agcattacca tcaacaatcc
    11221 acaaattcaa taaagatgtt tttgctaaga tgaaaaatcg aagctactta atcaacgctg
    11281 ctagaggccc aatcgtttct gaagaagcac ttattgaagc tctgaaagaa ggtgaaatcg
    11341 cgggtgcggg attagatgtt tttgagaatg aaccacaggt atctgaaggc ttgagaagtc
    11401 ttgataatgt tatcatgagt ccacatgctg ggacaggtac tattgaggga cgtagaactt
    11461 tggctgaaga agcagctgac aacatcattg ccttctttga tggcaagcca caaaatattg
    11521 tcaataagta attggctagt gagtgcagag ccatcttttg aaaaaatggg acctcccatt
    11581 tttttacggg taaatgtaag cgcttaataa tttaataaag gtgaacttat gctagaagct
    11641 ttacataaat tctttgaagt ttttggtgca gcaactgttg tacccgctat gatttttatt
    11701 gtcagccttt ttctaaaggt caatccaagg cgagctttct ttagcgcctt acgagctggt
    11761 gttggactca ctggctttgg ttggattata tcagcttttg ctcctatggt gactaagtat
    11821 atccagcaga tggttaaaac tactggtctc catttaccaa ttgtagatat tggttggcag
    11881 gctggttctc tcaccgcctt ttcttctgag attggcctct ctttctttgt ttttggcttg
    11941 ctaattgaat tggggctttt cttgcttggg attacccgag tatttgtccc atctaatctt
    12001 tggaataact ttggctatat gatttggggt acaatggcct atgcagcaac aggtaatttt
    12061 atattgtctt tcgcatttat ggtttttgtc ttactctatt cattagtgat gtcagaagta
    12121 ttggcagatc gatggtcgga gtattacggt gttaaaaatg caacgatcaa ctcgattcat
    12181 aatattgaaa cccttattcc agctcttatt cttgatcctc tttggaacct acttggtgtc
    12241 aataaggtaa agttgaatcc agaatcatta aagactaagt tagggatatt tggtgagcca
    12301 atgacacttg gttttatact tggtgtgatt attggtgtat taggtagttt acgtaacctt
    12361 gcaagtattg atacttgggg aggtatactt ggttttgcag ttgctcttgc agcggttatg
    12421 actatttttc ctttgattac gggggtattt gctagtgctt ttgctcctct tgctgaagca
    12481 gttgaacgca ataagaaaaa agaaagtcaa gctgagcaag gagcactgga taaaaaacgt
    12541 tggtttattg cagtagatga cggtgttggt tttggagaac cagcaactat tatcgcaggt
    12601 ctcattttag taccaataat ggttgttatt tccttgattt tacctggaaa tgaagcttta
    12661 ccagtcgttg accttattgc tattcctttt atgattgaag ctatgatagc tgtgtcaaaa
    12721 ggaaatatcc ttaaagctat tttgaatgga atcatatggt tttcactagg tctttatgct
    12781 gctagtgccc taggtccaat ctacacagaa gctgttaagc attttggaac tgctttacca
    12841 gcaggggtaa ctttgattat gagctttaat ctcctagctc atcctttgac agctctagtc
    12901 ttctttgctt ggatatctgg gaatccactc tatattggtg tgacagttat tatctatctt
    12961 gctttcttgt tcttgcttcg tactcgtcgt gaatctattt acgcatacct taaaactatg
    13021 gctgataaga atagcggata tgaaggtagt tgtgaaaaaa tctttattgc tgaagattaa
    13081 aaatctgttg gaggaatact catgaaaaaa attgatggac atgctcatat tgttgataca
    13141 attgcaggat ttaatggtaa agggcgtctt aatgcccttg gaaacggtta tgctatttgg
    13201 gatgatggtg atttaattaa gctcatccca gacgaatacg gtgacaaagc atttacagct
    13261 gaatcttttt tgaagtatat ggatgctaat ggtgtagata aagcagtcat tttgcaaggt
    13321 catcttaatg gttatcaaaa ttattatacg catttagcta ttaaacgtta tccagaacgt
    13381 tttacaggcg cattctctgt tgatccattt gcagataatg caatgcaaat tgtaaaacgt
    13441 catgttgaag tccttggttt tcgagctatt aaatttgaaa ttagtcaagg agggggtatc
    13501 catggatacc gtggacaaaa aacgcctttc cgtcttgata cagacccaca tgtgagtcgt
    13561 attttgactt atctgttaga ctatccagga tttgtagtta cagttgatta tggcaattgg
    13621 gatcaaatta gtcaccaacc tgatgctatt gctaatttag ctcgccttta tacgagtctc
    13681 gattttgttg tttgtcacct ctcttttcca catgttgagc attcaaatcg attgcgtgct
    13741 gaacttaata tgtggaaaaa cttcaacaat atctatactg atatttcagc tattcaagat
    13801 attgatgccc cagatagttt tccttttcca aaatcagaac aaaatgtacg tattgctaaa
    13861 gaagttcttg gggctaagcg tatcatttgg ggaactgatt ctccatggtc agcgaccttc
    13921 aatacttacg aagaattagc tacttggcta gagaatgttg atattttcag tcaagaggaa
    13981 ctagaagatg ttatgtacaa taatgctgag cgtgtttact ttaaagaaga acatgtaaag
    14041 gctaacagag caggtataga tgatacaacc aaagagctca atctttatta gaagagtttt
    14101 caaggtacaa gagtttcttg tacctccttt ttaaatcaac aatgaggaga caaattatga
    14161 cttactacct tagcattgac tacggcggta caaataccaa ggcgcttatt tttgacaaat
    14221 taggacacca aatcgctgtt tcgagttttg aaactttaaa aaatgaggct caatctggtc
    14281 atcgtcaagt aaaccttgtt aaaacgtgga atgctataac ttctgctatt agagaggtta
    14341 ttcaaatctc aaaactcagc cctgagcaga ttagtgcagt agcatgtatt ggacatggga
    14401 aaggtcttta tctgctagat aataagttgg agccacttga acaaggaatt ttgtctacag
    14461 ataatcgtgc caaagatttg gcgcaatatt tcgaatctaa acttgataat atttgggagt
    14521 tgactcggca acatattttc ccttcacaaa gtccagttat tttacgttgg ctcaaagatt
    14581 atcagcccga aacctataaa tcaatagggg cggtcctttc tgcaaaggac tttattcggt
    14641 ataagcttac aggaaaagta cagcaagaat atggtgacgc ttcaggtaat cattggataa
    14701 attttcaaac aggaacttat gatccagcta ttttagattt ttttggcatt agagagatag
    14761 aaaactcact tcctgaactt atagatagtg cagatttagt tcctggggga attagttctc
    14821 aagcagcaaa agagactggt cttgtagaag gaacccccgt tgttggaggg ctctttgaca
    14881 tcgatgcttg tgctcttgga tcaggtgttt tagagtcaga tacttttagt gttatttcgg
    14941 gaacttggaa tattaatact tatccaagtt taaaaccagc aaagcaagat agtggtctta
    15001 tgacttccta ttttccagat cgtcgttatc tcttagaggc aagtagccct acttctgcag
    15061 ggaatcttga ttttatgtta aaaatgctca tgcatcaaga aattgataac gctaaatcta
    15121 gtggaggttc tatctatgat aatttagaag aatttctcac tcatactgat gccacacatc
    15181 atggacttat tttctttccg tttctttacg gtagtaacac atcacaagat gctagcgctt
    15241 gcttttttgg gctaacaact aaatcgacga aatctcagat gatacgtgcg gtatatgaag
    15301 gtattgcgtt tgcacataag cagcatatca ctgatttaat aaaaagtagg ggcggtgtgc
    15361 caaaaataat tcgtttctct ggcggagcta ccaactcacc agcatggatg caaatgtttt
    15421 ctgatatctt aaaccttcct attgaaacag tagaaggcac agaattagga ggattaggag
    15481 gagctatttt agcacgtcat gctttagata agatttcgtt aaaggaagca gtccaagata
    15541 tggttcgtgt aaaagctatt tataaacctc aattatccga agtaaagggg tacgaaaaaa
    15601 aatatcacgc ctaccaaaaa ttattggaaa cactggatcc tatttggtcg gaactcggtc
    15661 atctgaataa gtagggatat caaaggagtt gttatgaaac aagaagatat tattatcaat
    15721 agtattgtat tcaaaaaaca gttagacaaa ggttttacac aagttcagct cattaagtta
    15781 gtaaaggatt taggattttc acgatttgag atacgtcaag aattgcttca agatccagat
    15841 agagagctac ccgctttaaa agcagaggct gacttttatg atatcaatct ttactatagt
    15901 gctaatgaag accttatcaa aggagggaaa gttaatcctt atcttaataa aggccttaaa
    15961 gaagcatcac agttaggagc tcctttcatt aagctaaatg ttggacaaac cagaaactta
    16021 agtaaagaag agttagaacc tcttaaagag atattgaaaa gccaaactat tggtataaag
    16081 gtggaaaata atcaagaccc taaagcagct acagtggaga attgccaata ttttatgact
    16141 ctagtgaaag aattacaaat accaataagt tttgtttttg atactgctaa ttgggccttt
    16201 atcaaccaag acctatacca agcggtaaat aacttagctt gtgataccac ctaccttcac
    16261 tgtaaaaatt ttatacaggt agctggaaag ccccacctct cgaagagcct cttcgaagga
    16321 gagattaatc tgactgattt gttaaagagt ttctctaatt gtgagtatct agctctagaa
    16381 taccccacag agttggagat attaaaaagg gatgttcaac gtttaatctc tatcagtaat
    16441 agtcaaaact tttaatgaaa aggctttaaa aagatgttat aataagtctg tatgaattcg
    16501 agtagaaaag aggtatattc ttgattattc tcgataaaaa aagttatgac ctcctctttt
    16561 acctattgaa attagaggaa cctgaaacag ttatggcaat tgccaacgca cttaatcagt
    16621 ctagacgtaa agtgtattat cacttagaga agataaacga tgcactgcct agcgatgtgc
    16681 ctcagattgt tagttatcca cgtgtaggaa tcttgctaac agaaaaacaa aaagcagcct
    16741 gtcgtctttt attagatgaa gtgactgatt atagttacgt catgaaaagt agtgagaggt
    16801 tgcagctgtc tttagtatct atcgtagtag ctaaggaccg cataacgatt gataggttga
    16861 tgcaactaaa cgatgtttct cgaaatacta tcttaaacga tttaaacgaa ttaagaagtg
    16921 agttagcaga gaaagaatat aatttacagt tacaatcaac aaaatgtcgt ggttattttt
    16981 tagatggtca tccattgtcc attatccagt acttatataa gctcttagat gatatctacc
    17041 ataatggaag tagtagtttt atagaccttt ttaatcataa actgtctcaa gcttttggtg
    17101 ccagcactta tttttctaaa gaggttcttg attattttca tcattatctc ttcatttctc
    17161 aacgaagtct aggtaagaaa atcaacagtc aagatggtca gtttatgatt cagattttgc
    17221 cttttatact aatggcttat cgtaagatgc gattaagtcc tgaagtacag acctctctta
    17281 atagtgattt tagcttggtt tggcaacgta aggaatatga gattgctaaa gagttggctg
    17341 atgagctgga agaaaatttt cagttatcac tggatgagat tgaagtggga ctagtagcca
    17401 tgcttatgct tagtttccgc aaggaccgtg acaatcattt agagagccag gattatgatg
    17461 atatgcgagc tactctaacc agttttttga aagaattgga agaacgatat caccttcact
    17521 ttgttcataa aaaggactta ctaagacaac ttcttactca ctgcaaggca ctcttatatc
    17581 gtaaacgtta tggtattttt tctgttaatc ctttaacaga gcatattaaa gacaaatatg
    17641 aagaactttt tgccataacc tcgtcttctg taaagctttt agagaaagct tggcaaatca
    17701 aattgaccga tgatgatgta gcatatctaa cgattcattt aggaggggaa cttcgtaata
    17761 gtcaacaatc tcctaataaa cttaagttag ttattgtatc tgatgaagga atagcgattc
    17821 agaaacttct tttaaagcaa tgtcaacgct acttaacaaa tagtgatata gaagctgttt
    17881 ttacaaccga acagtatcaa agtgtgagtg atcttatgca tgtagatatg gttgtctcta
    17941 ctagtgacgc tttagaatct cgttttccga tgttagtagt tcaccctgtt ttgacagatg
    18001 atgatattat tcgcttgatt cgcttttcta aaaaaggtaa ctgtgcaaat agtaatcaat
    18061 ttaccaatga acttgaaaaa acaattgctc aatatgtcaa ggaagatagt gaacgctacg
    18121 tgctgaaatc taagattgag aaacttattc atcaagaatt gctccaagac gtccttcccc
    18181 ttcaaagtac agtttgttaa aatataattt tatggtagcc tctcagcttt tctgaagaca
    18241 gaaaaagaag ctggggcttt tttggtgctt ttaaacaatg tgctagatat ttaatcgcaa
    18301 tgagttcctc tatgacatga taattaaagt tgagaaagct agtactttta atgggagtac
    18361 tttttatttg tgcttatttt aattaaaatt atgagtctaa tttggaacag acagcccagt
    18421 tttgaacaca taaatgtgtg acacttggtc tttttttcaa aagtatttga acttatacta
    18481 gtagatgtaa gaaacaatta atcaggagga aataaaatgc ctaacgttaa agatattact
    18541 agagaatcat ggattttatc aacattccca gaatggggta catggctaaa cgaagaaatt
    18601 gaagaagaag ttgttgctga gggcaacttt gcaatgtggt ggttaggaaa ctgtggtgtt
    18661 tggattaaaa caccaggtgg tgctaatgtt gttatggacc tatggtcaaa ccgcggtaaa
    18721 tcaacaaaaa aagtaaaaga tatggttcgt ggacaccaaa tggctaatat ggcaggtgtt
    18781 cgtaaactcc aaccaaactt acgcgcacaa ccaatggtta ttgatccatt tgctattaat
    18841 gaattggatt actatcttgt ttcacatttc cacagtgacc acattgatat caacactgcg
    18901 gcagccatta ttaataatcc taacttagac catgttaaat ttgttgggcc atatgaatgt
    18961 ggtgaaattt ggaaaaaatg gggagttcca gaagaacgaa tcatcgtgat taaaccaggt
    19021 gaatcatttg agtttaaaga tatcaaagtt actgctgttg aatcatttga ccgtacttgc
    19081 ttggtgacac ttccagtaga tggtgcagaa gaacatgatg gagaacttgc tggtttagcc
    19141 gtgactgatg aagaaatggc tcgtaaagca gttaactata tttttgaaac accaggtgga
    19201 acaatctatc atggagctga ctcacatttt tcaaattatt ttgctaaaca tggtaaagac
    19261 tacaagattg acgtagctat taacaactat ggtgataacc cagttggtat tcaggataaa
    19321 atgacttcaa tcgatcttct tcgtatggca gaaaatctcc gtgccaaagt cattattcct
    19381 gtacactacg atatctggtc taacttcatg gcttctacag acgaaatctt acaattgtgg
    19441 aaaatgcgta aagaacgcct tcaatatgac ttccatccat ttatttggga agttggaggt
    19501 aaatacactt atccacaaga taaagataga attgaatacc atcacccacg tggttttgat
    19561 gattgcttcg aacaagaatc aaatattcaa tttaaagcac ttctataatg gctaatgaga
    19621 gactgtatgt cgcatgatat gcagtttttc aattttaaaa tatgttacta aaatcacaat
    19681 taagattagg agaaataatg tcagaatttg atacaaaatc atatttagaa aaacttgatg
    19741 catggtggag agcagctaat tatatttctg cagcacaaat gtatctaaag gataaccctc
    19801 tcttgagacg agagctggtt gaaaacgact taaaggttca tccaattggt cactggggca
    19861 ctgtacctgg acaaaatttt atctatgctc acttaaatcg cgctattaat aaatacgatt
    19921 tagacatgtt ctatattgaa ggtccaggtc atggtggtca agttatggta tctaattcat
    19981 atttagatgg ttcatatact gaattaaacc caaatattga gcaaacagag gatggcttta
    20041 agcagttatg taaaatcttc tctttcccag gtggaattgc atcccatgct gcaccagaaa
    20101 caccagggtc aattcatgaa ggcggtgaac ttggttacgc actttctcat gcgacaggtg
    20161 ccatcctaga caatccagat gttattgctg cgactgttat tggtgatggt gaaggtgaaa
    20221 caggaccact tatggctggt tggctatcta atacctttat caatccagta aatgatggtg
    20281 ctgttctacc aatcttctat ttaaatggtg gtaaaatcca taacccaact atctttgaac
    20341 gtaagacaga tgaagaatta tctcagtttt ttgaaggatt aggttggaaa cctatttttg
    20401 cagatgttgt tgaactctct gaggatcatg cggctgctca tgctttgttt gcagaaaaat
    20461 tagatcaagc tattcaagag attaaaacca ttcaatcaga agcacgacaa aaaccagcag
    20521 aagaagctat ccaagcaaaa ttccctgtct tagttgcacg tattcctaag ggttggactg
    20581 gtccaaaagc ttgggaagga acaccaattg aaggtggctt ccgcgctcac caagtaccaa
    20641 ttccagtaga tgcccaccat atggaacatg ttgattctct tttgtcatgg cttcaatcat
    20701 accgtccaga agaattattt gatgaaaatg gtaaaatcgt tgatgagatt gctgctattt
    20761 caccaaaagg tgatcgtcgc atgtccatga atccaataac caatgctggt attgttaaag
    20821 caatggatac agcagattgg aagaaatttg ctcttgatat taatgttcca ggtcaaatta
    20881 tggcacaaga tatgattgaa tttggaaaat atgcagcaga tttggtagat gctaatccag
    20941 ataatttccg tatttttggt ccagatgaaa cgaaatcaaa tcgtcttcaa gaagtgttta
    21001 cacgtactag ccgtcaatgg cttggtcgtc gtaaaccaga ttatgacgaa gctttaagtc
    21061 cagctggacg tgttattgat tcccaattgt ctgaacacca agcagaaggt ttcttagaag
    21121 gttatgtttt aactggtcgt cacggcttct ttgcttcata cgaatcattc cttcgcgttg
    21181 tagattcaat ggtaacacaa catttcaaat ggttacgtaa atcaaaaaca cacacaacat
    21241 ggcgtaaaaa ttatccagcg cttaacctaa ttgcagcttc aactgttttc caacaagatc
    21301 ataatggtta tactcaccaa gatccaggta ttttaactca tttagctgaa aaaacacctg
    21361 aatacattcg tgaatattta ccagcagata ctaactcact tctagctgtt atggataaag
    21421 cgtttaaagc tgaagataaa attaatttaa ttgtgacatc taagcaccct cgtccacaat
    21481 tttactctat tgctgaagca gaagagttag tggcagaagg atataaggtg attgactggg
    21541 cttcaaatgt gtcgcttaat caagagccag atgttgtctt tgctgcggcg ggaacagaac
    21601 ctaacttaga agctttggca gctattagca ttcttcataa ggctttccca gaacttaaga
    21661 ttagatttgt taatgtatta gacatcctaa aacttcgtca tccttcacaa gatgctcgcg
    21721 gtttatcaga tgaagagttt aacaaagtct ttaccacaga taaacctgtt atttttgctt
    21781 tccatggtta tgaagatatg attcgagata ttttctttag tcgtcacaat cataatttgc
    21841 atacgcatgg ttaccgtgaa aatggtgaca tcacaacacc atttgatatg cgcgtgatgt
    21901 cagaattgga ccgattccat ctggcacagg atgcagctct tgcttcttta ggaaataaag
    21961 cacaagcctt cagtgatgaa atgaatcaaa tggttgctta tcacaaagac tatattcgtg
    22021 aacacggaga cgatattcca gaagtacaaa attggaaatg ggaaaatatc aagtagtcac
    22081 ttttttcagg cctatactta caaacaaaaa cacttttaga ttttttctaa gggtgttttg
    22141 ttattttata gaagaatgac cgctttttca aaagtattaa gtggtcactt ttaaagttgt
    22201 aatattaata ctaataagtc tttaaataat tcaataatgc cataacaata atggtgtggg
    22261 ctaattaaaa tatctctaag caaaggtgtt gacataaaga gaccatacat gatattatag
    22321 ttatgttctg tttcggcagt ttattttttt gagtaaaaaa gtgactaccc ttttacgata
    22381 acttaacgtc actttataga taagacacat aaccttaaaa ataagatacg aaatttttag
    22441 taggagataa gaatgacaaa tattttagaa gtgaagaatt taaccaaaat ttttgggaaa
    22501 aagcaaaaag ctgctcttga aatggttaag caaggaaaaa gtaagacaga aattcttgaa
    22561 aaaacaggag cgactgtagg tgtctatgat gcgagttttg aaataaaaga aggcgaaatt
    22621 ttcgttatca tgggactttc tggttcagga aaatcaactt tagttcggat gttaaaccgt
    22681 ttgattgacc catcatcagg aaatatctat ttagatggta aagatattgc aaagatgaat
    22741 gtagaagatt tacgtaacat tcgtcgtcat gatatcaaca tggttttcca aaattttggt
    22801 cttttcccac atcgtacaat tttagaaaat acagagtttg ggttagagat gcgtggtgtt
    22861 tctaaggaag aacgtacaac gttagctgaa aaagctttgg acaatgcagg tctattaccg
    22921 tttaaagatc agtaccccag tcaattatca ggaggaatgc aacagcgtgt tggtttagca
    22981 cgtgcccttg ctaacagccc taaaatcctt ctgatggatg aggccttctc agctttagac
    23041 ccattaattc gtcgtgaaat gcaagatgag cttcttgatc ttcaagacac gaataaacaa
    23101 actattattt ttatcagcca tgatttgaat gaagcgttac gtattggtga tcgtattgct
    23161 ctgatgaaag atggagaaat catgcagatt ggtacaggag aagagatttt gactaatccg
    23221 gctaatgatt ttgttcgtga gtttgttgaa gacgttgatc gctcaaaagt gttaactgct
    23281 caaaatatta tgataaaacc tttaacaact gttttagaaa ttgatggccc acaagttgct
    23341 ttaacaagaa tgcatcgtga ggaagtctca atgttaatgg caacgaatcg tcgtcgccaa
    23401 cttctgggat ccctaactgc agatgctgca attgaagctc gtaagaaagg tttgccgctt
    23461 tctgaagtga ttgataaaga tgttgtaacg gtctcaaaag atacagttat tacagatatc
    23521 atgcctttaa tttatgattc atctgctccg atagccgtta ctgatgataa tgatagactc
    23581 ctaggggtta tcattagagg acgtgtgatc gaagcacttg ctaacgttca agatgaaaca
    23641 gtagtagaat cgccaaaaga aacggtagaa gcttaataga gaggacaatt cattttggaa
    23701 aatttattac aacataagtt accggtcgca ccatttgtag agtcaacgac caattggatt
    23761 acgaaaactt tttctggttt atttgacttt atccagacaa tcggtaatgc attgatggat
    23821 tggatgacaa aaacattact attcattaat cctttacttt ttattgtact aattacaatt
    23881 gctgtatttt tcttagctaa gaaaaaatgg caattaccga catttacttt cattggtttg
    23941 ctatttatct ataaccaagg gctgtgggaa cagttgatta atacttttaa cttggtttta
    24001 gtagcaagtt taatttctat tattattggt gtaccactag gtatttggat ggcaaaaagt
    24061 gataaagtta aacaagttgt taatccaatt cttgacttta tgcaaactat gccggcattc
    24121 gtttacctta ttcctgcagt agccttcttt ggtatcggta tggtaccagg tgtttttgcc
    24181 tctgttgtat ttgctctgcc tccaacagtg cgctttacta acttggctat tcgtgaaatc
    24241 ccacttgaat taatagaagc atctgattca tttggttcaa cagctaagca aaaattattc
    24301 aaagttgaac taccacttgc taagaacact attatggcag gaatcaatca aactatgatg
    24361 ttggctcttt ctatggtggt tacaggatct atgatcggtg cacctggact tggtcgtgag
    24421 gtgttatcgg cgcttcaaca tgctgatatt ggtacaggat ttgttagtgg tctatcgcta
    24481 gttattttag caatcgtgtt agaccgtgtt agtcaattct ttaattccaa accgggtgag
    24541 aagcaagcta aaacatcaaa agttaaaaaa tgggtaggtt taggggccct ggctctcttc
    24601 attctagcag ctcttggtcg cattgttgtc aatatgactt caggaaatga agccaaaggt
    24661 caaaaagtga agatagctta tgtccaatgg gattcagaag ttgcttcaac caacgttatt
    24721 gctgaagttt taaaatctaa aggatatgat gttgaattga caccacttga caatgcggtc
    24781 atgtggcaaa cagtcgccaa tgggaatgct gattttacaa caagtgcttg gttaccaaag
    24841 acccacgggc aatattttaa taaatacaaa aatagtttag atgacttagg tcctcacgtt
    24901 gagaacgtca aaataggttt agttgttcct aaatacatga acgtaaatag tattgaagaa
    24961 ttatcaaatc aagcagataa acaaattaca ggtatcgaac caggtgcagg tattatgaag
    25021 tctgctaaac aatcccttaa agattatcca aacctttcaa gttggaagtt gttatcagca
    25081 tcaacaggag ctatgacaac aaccttaggg aaggctatca aaaacaaaga tcaagtagtc
    25141 attacaggtt ggtcacctca ctggatgttt gctaagtatg atttaaaata ccttaaagat
    25201 cctaagaaat catttggtgg tgaagaacat atcaatacaa tagcacgcaa gaatttgaaa
    25261 aaagatatgc caaaagtata taaaattatc gataagttga agtggactaa agaagatatg
    25321 gaatctatca tgttagatat ggataaaggt atggagcctg ctaaagcagc tcaaaaatgg
    25381 attaaaaacc acaaaaaaga agtttcagag tggactaaat agtatagaaa aaaacacgtc
    25441 tctcttagag cgtgtttttt aatgtgactt aagtcgtttt agctatttaa tataagtttt
    25501 atattatttt aatacatcaa aagtgagaga atgaaagaaa tggcaggtta taaagtatta
    25561 c
//
LOCUS       DATRUK010000024        25484 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.024, whole genome shotgun sequence.
ACCESSION   DATRUK010000024 DATRUK010000000
VERSION     DATRUK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25484
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.024"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(338..952)
                     /locus_tag="VBE34_000967"
     CDS             complement(338..952)
                     /locus_tag="VBE34_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO7263695.1"
                     /translation="MSFLEELKNRRSIYALGRNTEVSDEKIVEIIKEAVRQSPSAFNS
                     QTSRVVILLNDEVTKFWDELVANDLVETMKVQGAPETAIAGTKEKLASFGASKGTVLF
                     FEDQDVVKSLQEQFVLYADNFPVWSEQSTGIASVNTWTALSAELGLGGNLQHYNPVID
                     ASVQAVYGVPASWKLRGQLNFGSIEAETGEKEFMNDDDRFKVIG"
     gene            complement(1085..1510)
                     /locus_tag="VBE34_000968"
     CDS             complement(1085..1510)
                     /locus_tag="VBE34_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7263696.1"
                     /translation="MGNFKNSAVKSMVVMRKAFRTIDGKVSESFKEFELTPTQFAVLD
                     VLYAKGTMKIGELIENMLATSGNMTVVIKNMEKKGWVLRHSCPNDKRAFLVSLTTEGE
                     EVIKKALPEHIKRVEDAFSVLTETEQEDLINLLKKFKTL"
     gene            complement(1637..6043)
                     /locus_tag="VBE34_000969"
     CDS             complement(1637..6043)
                     /locus_tag="VBE34_000969"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEO7263697.1"
                     /translation="MSELFKKLMDQIEMPLEIKNSSVFSSADIIEVKVHSLSRLWEFH
                     FSFPELLPIEVYCELQTRLVNSFEKADIKATFDIRAETIDFSDDLLQDYYQQAFCEPL
                     CNSASFKSSFSQLKVHYNGSQMIISAPQFVNNNHFRQNHLPRLEQQFSLFGFGKLAID
                     MVSDEQMTQNLKSSFETNREQLLEKANQEAMQALEAQKSLEDSAPPSEEVTPTQNYDF
                     KERIKQRQAGFEKAEITPMIEVTTEENRIVFEGMVFSVERKTTRTGRHIINFKMTDYT
                     SSFAMQKWAKDDEELKKYDMISKGSWLRVRGNIENNNFTKSLTMNVQDIKEIVHHERK
                     DLMPADQKRVEFHAHTNMSTMDALPTVESLIDTAAKWGHPAIAITDHANVQSFPHGYH
                     RAKKAGIKAIFGLEANIVEDKVPISYNEVDMNLHEATYVVFDVETTGLSAANNDLIQI
                     AASKMFKGNIIEQFDEFIDPGHPLSDFTTELTGITDNHVRGSKPILQVLQEFQNFCQG
                     TVLVAHNATFDVGFMNANYERHNLPLITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQ
                     VALEHHHMANYDAEATGRLLFIFLKEARENRDVTNLMELNTKLVAEDSYKKARIKHAT
                     IYVQNQVGLKNIFKLVSLSNVKYFEGVARIPRSVLDAHREGLLLGTACSDGEVFDALL
                     SNGIDAAVTLAKYYDFIEVMPPAIYRPLVVRDLIKDEAGIQQIIRDLIEVGRRLDKPV
                     LATGNVHYIEPEDEIYREIIVRSLGQGAMINRTIGRGEDAQPAPLPKAHFRTTNEMLD
                     EFAFLGKDLAYEIVVTNTNTFADRFEDVEVVKGDLYTPFVDRAEERVAELTYAKAFEI
                     YGNPLPDIIDLRIEKELASILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVA
                     TMIGITEVNPMPPHYVCPNCQHSEFITDGSCGSGYDLPNKNCPKCGTLYKKDGQDIPF
                     ETFLGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGEEYAFRAGTVGTVAEKTAFGFVK
                     GYERDYNKFYNDAEVERLATGAAGVKRSTGQHPGGIVVIPNYMDVYDFTPVQYPADDM
                     TAAWQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPSNILPDDPDVMKLFS
                     GTEVLGVTEEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWL
                     GNAQDLIKEGIATLSTVIGCRDDIMVYLMHAGLQPKMAFTIMERVRKGLWLKISEDER
                     NGYIQAMRDNNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHYPIFYYCAYF
                     SIRAKAFELRTMSAGLDAVKARMKDITEKRQRNEATNVENDLFTTLELVNEMLERGFK
                     FGKLDLYRSHATDFIIEEDTLIPPFVAMEGLGENVAKQIVRAREDGEFLSKTELRKRG
                     GVSSTLVEKFDEMGILGNLPEDNQLSLFDDFF"
     gene            complement(6166..6750)
                     /locus_tag="VBE34_000970"
     CDS             complement(6166..6750)
                     /locus_tag="VBE34_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEO7263698.1"
                     /translation="MKSRKKDKLVLRLTTTLLVFGLGGVWFYNYKNDNVEPTVTSASD
                     QTTTFIQTISPTAIEISKTYDLYASVLLAQAILESSSGQSDLSKAPNYNLFGIKGEYK
                     GKSVQMPTLEDDGKGNMTQIQAPFRAYPNYSASLYDYAELVSSQKYASVWKSNTSSYK
                     DATAALTGLYATDTAYASKLNQIIETYSLDAYDK"
     gene            complement(6875..8728)
                     /locus_tag="VBE34_000971"
     CDS             complement(6875..8728)
                     /locus_tag="VBE34_000971"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEO7263699.1"
                     /translation="MKQSKMLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMRQEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDQS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHKDYEDLDVTYEDYRKAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAVTPNRT
                     DLNRWLVLDKTIPSIDDIPEDVLEEIKAELSAWLVSGEDTIAYSTESSYAANLEMATN
                     EYKPSTKAATFEEVTKVETPNCKSIDEVAGFLSIDENQTIKTLLFIADEQPVVALLVG
                     NDQVNDVKLKNYLAADFLEPASEEQAKEIFGAGFGSLGPVNLPESVKIIADRKVQDLA
                     NAVSGANQDGYHFTGVNPERDFTAEYVDIREVKEGEISPDGKGTLKFARGIEIGHIFK
                     LGTRYSDSMGANILDENGRSNPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGAYRF
                     AWGINFPEELAPFDVHLITVNVKDQESQDLTEKIEADLMLKGYEVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKASEGIVEVKIKASGDTIEVHADNLIETLEILTKK"
     gene            complement(8820..10079)
                     /gene="rseP"
                     /locus_tag="VBE34_000972"
     CDS             complement(8820..10079)
                     /gene="rseP"
                     /locus_tag="VBE34_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEO7263700.1"
                     /translation="MLGILTFIIIFGVIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHIDKEGTTYTIRILPLGGYVRMAGWGDDKTEIKTGTPASLTLNKEGIVTRINLSGK
                     QLDNTSLPINVTAYDLEDKLTITGLVLSETKTYSVDHDATIIEEDGTEIRIAPLDVQY
                     QNASVWGRLITNFAGPMNNFILGLVVFIALAFIQGGVQDLSTNQVRVSENGPAASAGL
                     KNNDRILQIGSHKVSNWEQLTAAVEKSTSHLEKNQKLALKIKSKEVVKTINVKPQKVD
                     KSYIIGIMPALKTSFKDKLLGGFKLAWESFFRILNELKKLIAHFSINKLGGPVALYQA
                     SSQAAKNGFVTVLNLMGLISINLGIMNLIPIPALDGGKIVMNILEAIRRKPLKQETET
                     YITLAGVAVMLVLMIAVTWNDIMRAFF"
     gene            complement(10110..10904)
                     /locus_tag="VBE34_000973"
     CDS             complement(10110..10904)
                     /locus_tag="VBE34_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEO7263701.1"
                     /translation="MKERVIWGAVALAIFIPFLVMGGLPFQFLVGLLAMIGVSELLRM
                     RRLEIFSFEGALAMIGAFVLTVPLDSYLSFLPVDASLSAYGIVIFMILAGTVLNSNSY
                     SFEDAAFPIASSFYVGIGFQNLVSARMAGIDKVLLALFIVWATDIGAYMIGRQFGQRK
                     LLPSVSPNKTIEGSLGGIASAIVVAFFFMLFDKTVYAPHSFLVMLVLVAIFSIFGQFG
                     DLVESSIKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHFFGLF"
     gene            complement(10919..11671)
                     /locus_tag="VBE34_000974"
     CDS             complement(10919..11671)
                     /locus_tag="VBE34_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEO7263702.1"
                     /translation="MFIFKRKKQVEMPLEKIPAHIGIIMDGNGRWAKKRLKPRVMGHK
                     AGMDALQEVTIAASELGVKVLTVYAFSTENWSRPDDEVKFIMNLPVEFFDKYVPELDK
                     NNVRVQVIGDTHKLPKATYDAMQRACLRTKHNSGLVLNFALNYGGRSEITNAIKEIAQ
                     DVLEAKLNPDDITEDLVANHLMTNSLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEF
                     YFTPVLWPDFKKDELHKAIVDYNQRHRRFGSV"
     gene            complement(11838..12179)
                     /gene="yajC"
                     /locus_tag="VBE34_000975"
     CDS             complement(11838..12179)
                     /gene="yajC"
                     /locus_tag="VBE34_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEO7263703.1"
                     /translation="MSLPIIMLVVMVGMMFFMQRQQKKQAQERQKQLNAVQKGDEIVT
                     IGGLFGVVDEVNTEAQRMVLDVDGVYLTFELAAIKSVVSKAATPTEPVEDVKIIEVNE
                     DENKETAIETE"
     gene            complement(12256..12600)
                     /locus_tag="VBE34_000976"
     CDS             complement(12256..12600)
                     /locus_tag="VBE34_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEO7263704.1"
                     /translation="MYSFEELLATMTLITAAEIEDKIDSNQDFVLFIGRISCPFCHLF
                     VPKIVEVADEDEFELFHLDSEDFDHWTANKDFRNKYDIPTVPGLMVVKNGTIKVKCDS
                     KMTKEEIREFIG"
     gene            complement(12791..13954)
                     /locus_tag="VBE34_000977"
     CDS             complement(12791..13954)
                     /locus_tag="VBE34_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEO7263705.1"
                     /translation="MSENLGQLAINQARENGGKLEVISKVKVEDKRDLSIAYTPGVAS
                     VSSAIAEDVELAYELTTKKNTVAVVSDGSAVLGLGDIGPEAAMPVMEGKAALFKRFAN
                     VDAVPIVLKTNDTEEIISIVKAISPTFGGINLEDISAPRCFEIEQRLIEECDIPVFHD
                     DQHGTAIVVLAALFNSLKLVKKDIEDIKVVVNGGGSAGLSITRKLLSAGAKHVTVVDR
                     FGIINDKDRESLAPHHKAIAKLTNREFQSGSLEDALENADVFIGVSAPEALHAEWISK
                     MADKPIVFAMANPIPEIYPDQALKAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAARGIASLIPEEELSTTHIIPNAFQNDVADVVAKSVSNAVQK"
     gene            complement(13979..15316)
                     /locus_tag="VBE34_000978"
     CDS             complement(13979..15316)
                     /locus_tag="VBE34_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEO7263706.1"
                     /translation="MADVKVVNNEGSRGQKQDLKTKLFHIKVGSVPLPVYVCLALLIL
                     LAGFLQKLPVNMLGGFAVILTMGWFLGTIGASIPGFKNFGGPAILSLLVPSILVFFNL
                     INKNVLESTNMLMKQANFLYFYIACLVSGSILGMNRKMLIQGLLRMIFPMLLGMVCAM
                     MVGTFVGVILGLEWRHTLFYIVTPVLAGGIGEGILPLSLGYSSITGVASEQLVAQLIP
                     ATIIGNFFAILCTALLNRLGEKKPHLSGQGQLVRLNKGEDMSDIIADHSGPIDVKKMG
                     GGVLTACSLFIFGHLLQQLTGFPGPVLMIIAAAILKYINVIPRETQNGAKQLYKFISG
                     NFTFPLMAGLGLLYIPLKDVVATLSIQYFIVVISVVFTVISVGFFVSRFLNMNPVEAG
                     IISACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRMLF"
     gene            15470..16996
                     /locus_tag="VBE34_000979"
     CDS             15470..16996
                     /locus_tag="VBE34_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7263707.1"
                     /translation="MKKKLSLWAFLSLILVTMTICIFSIFYYVTIHQSYRMVRVQEEK
                     ILKNTGYALSRNPQVIQTLKDNHYNQSLQKQMLFLSKKSNLDYIVLINLKGIRFTHPD
                     STKIGKPFQGGDEQAVFKGKAIMSTAEGSLGKSLRYLIPVYDHQKQVGAIAVGLKLTT
                     LGDLSQSSIKEFSKPLLISILISLVVTSIISYGLKKQLHNLHPSDIFQHLEERNATLD
                     QIQAAVFVIDQRHIIKRNNPAASLLFKKEGQRDLFSGKLLESLIPQLKQDHFSKKTEQ
                     VLHFQGQDYLLSISPITVKTQNRGYVVFLRNVTETLFTLDQLAHTTAYASALQAQTHQ
                     FMNQLHVIYGLADIEYYDELKIYLKELLEPQNEFLARLSMLVREPRLASFIIGEREKF
                     AEKHINLSTEILVEIPTKSTVEDVNNYLLLHRYINTKILTLLNSTTLVSLRLNYQNNL
                     IETDYQWENEKWLLNDYHQYFNDAYFQQLLVDSRATYKISLKQNKVIIRTNIIYSEGS
                     "
     gene            16998..17678
                     /locus_tag="VBE34_000980"
     CDS             16998..17678
                     /locus_tag="VBE34_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO7263708.1"
                     /translation="MDVLIIEDDPMVEFIHRNYLEKLNYFQNIYSTASQTQAIAYLND
                     IKIQLVLLDIHIKEGNGLELLKLLRNQHQNTEVIVISAANEAHTVKEAFHLGIVDYLI
                     KPFTFERFESSIEKFLNHYHTFEADKIYQDNIDHFQKIDSGWLEGEVKLDEKGLSEIT
                     YQHILDAIQELEQPFTIQELAKCSQFSHVSVRKYIAYMEEKGLLTSQQIYTKVGRPYK
                     VYKLIKKT"
     gene            complement(17699..18694)
                     /gene="galE"
                     /locus_tag="VBE34_000981"
     CDS             complement(17699..18694)
                     /gene="galE"
                     /locus_tag="VBE34_000981"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEO7263709.1"
                     /translation="MAVLILGGAGYIGSHMVDQLITQGKEKVIVVDNLVTGHRQAVHS
                     DAIFYEGDLSDKTFMRQVFRENPDVDAVIHFAAFSLVAESMENPLKYFDNNTAGMIKL
                     LEVMNECDIKNIVFSSTAATYGIPEQVPILETAPQNPINPYGESKLMMETIMKWADQA
                     YGIKFVALRYFNVAGDKPDGSIGEDHKPETHLLPIILQVAQGVRDKIMIFGDDYNTPD
                     GTNVRDYVHPFDLADAHILAVDYLRQGNESNVFNLGSSTGFSNLQMLEAARRITGKEI
                     PAQKAARRPGDPDTLIASSEKARQMLGWEPKFDNIDKIISSAWAWHSSHPNGYED"
     gene            complement(18782..20389)
                     /locus_tag="VBE34_000982"
     CDS             complement(18782..20389)
                     /locus_tag="VBE34_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEO7263710.1"
                     /translation="MKKHWWHKATIYQIYPRSFMDSDGDGVGDIKGITSKLDYLEKLG
                     ITAIWLSPVYQSPMDDNGYDISDYQAIADIFGDMNDMDQLLQEANQRGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDKASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIDKGIGGFRMDVIDLIGKIPDKGITGNGPKLHDYL
                     KEMNRASFGKHDLLTVGETWGATPDIAKQYSNPDNEELSMVFQFEHVGLQHKPDAPKW
                     DYSDGLDVPALKDIFTKWQTQLELGQGWNSLFWNNHDLPRVLSIWGNDSDNRKQSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFECLADVDDIESLNYAKEAMDNGVSEATILDS
                     IRKVGRDNARTPMQWSQGHQAGFTNGTPWLAVNPNYQEINVEAALNDTESIFYTYQKL
                     VALRKEHDWLVDADFKLLETADKVFAYVRQTDKERYLIVANLSDQNQSFEFPEAVKET
                     IISNTEVQEVLSSNTLKPWDAFCIELF"
     gene            complement(20518..21651)
                     /gene="ugpC"
                     /locus_tag="VBE34_000983"
     CDS             complement(20518..21651)
                     /gene="ugpC"
                     /locus_tag="VBE34_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEO7263711.1"
                     /translation="MVELNLNHIYKKYPSASHYSVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIDGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKFSKQEIDKRVREAAANIGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHQRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPDGD
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPSMNFFKVKVENGMIISEDGLRIAIPEG
                     QEKLLESRGYKGKELIFGIRPEDISSNLLVQDTYPNANVEAEVLVSELLGSETMLYVK
                     LGQTEFASRVEARDFHNPGEKVNLTFNVAKGHFFDADTEQAIR"
     gene            complement(21752..22603)
                     /locus_tag="VBE34_000984"
     CDS             complement(21752..22603)
                     /locus_tag="VBE34_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7263712.1"
                     /translation="MDFKELFPEVITKQEVKQSEDYIIVEQDGHVLHFPKSSLTKREL
                     CLLQMTQSLEEASSVDSQNPWYRYLVEGRGRLPQSHSAVQFIFIEHQFTLSEELKDFL
                     SPLVINVETIMTINQTQSVMILNQDNFFNATELLTDILPTIENDFNTRLRCYFGNSWT
                     HLQAVDWKELYEEEYKLFTLFLSHKAEQHYCRFPKMALWALANQSPMPSIKAKCLQHI
                     LDTSDTSAIIKALWQEQGNLAKTAKALFIHRNSLQYKLDKFTQSSGLNLKILDDLAYA
                     YLISMDT"
     gene            complement(22698..23594)
                     /locus_tag="VBE34_000985"
     CDS             complement(22698..23594)
                     /locus_tag="VBE34_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEO7263713.1"
                     /translation="MAITIQNHELQVTLKALGATMTSITDSQGVEYLWQGEPTYWGGQ
                     APILFPICGSVRNDCVIYRPAQAPHFTGIIPRHGFVRHKTFDYDYISDSSVRFTIKSS
                     EETLINYPYRFSLEITYTLRNKSIAITYIVKNLESEKNMPYAIGAHPGFNCPLFEKEV
                     FSDYYLEFEQFETCTIPESFPDTGLLDLQARHPFLENQKQLSLNYALFEKDAITLDQL
                     RSKTVYLKSRNHAKGIQLDFDDFENLILWTSNNGGPFLALEPWSSLSTSIEESDILED
                     KQNIVRLNPKQSKQHSIRITIL"
     gene            complement(23648..24625)
                     /gene="lacD"
                     /locus_tag="VBE34_000986"
     CDS             complement(23648..24625)
                     /gene="lacD"
                     /locus_tag="VBE34_000986"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEO7263714.1"
                     /translation="MGLTEQKQKHMEQLSDKNGIISALAFDQRGALKRLMAKYQSEEP
                     TVSQIEALKVLVAEELTPYASSMLLDPEYGLPATKVLDDNAGLLLAYEKTGYDTSSTK
                     RLPDCLDIWSAKRIKEEGADAVKFLLYYDVDSSDEVNEEKEAYIERIGSECVAEDIPF
                     FLEILSYDEKITDSSGIEYAKIKPRKVIEAMKVFSNPRFNIDVLKVEVPVNMDYVEGF
                     AQGETAYNKATAAAYFREQDQATLLPYIFLSAGVPAQLFQETLVFAKEAGAKFNGVLC
                     GRATWAGSVKEYVEKGEAGARQWLRTIGFQNIDELNKILQKTATSWKER"
     gene            complement(24627..>25484)
                     /locus_tag="VBE34_000987"
     CDS             complement(24627..>25484)
                     /locus_tag="VBE34_000987"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEO7263715.1"
                     /translation="NRVTDVSKTPGGKGLNVTRVLSQLGDNVVATGLLGGHFGDFIRS
                     GLDALEIRHQFLSIGGETRHCIAVLHEGQQTEILEKGPHITKDEAGAFLNHLKLIFDA
                     ATIITVSGSLPKGLPSDYYARLISLANHFNKKVVLDCSGEALRSVLKSSAKPTVIKPN
                     LEELTQLIGKPISYSLDELKSTLQQDIFRGIDWVIVSLGARGAFAKHGNHYYQVTIPK
                     IEVINPVGSGDATVAGIASALEHQLDDINLLKRANVLGMLNAQETLTGHINLTYYQEL
                     ISQIQVKEV"
BASE COUNT         8148 a         5205 c         4045 g         8086 t
ORIGIN      
        1 agagaaagaa tatgaagaac tactacaaaa agatagatta ttcaagcatg agtacacaga
       61 gtttacaaaa agtctaaatt tgaatgaaac attctattca caattaaaag aaggagaaat
      121 gaaactaagt gaaaatcccg aaaaaggaga aacgaacacg aattaacctt tcaaatatct
      181 acatcaaata acagaagtct ccgtatcaag cgaattgtag cttttttgaa tatactaaaa
      241 acactcctac ctctatttta ctagaagtag gagtgttttt ttcattttag ttcatagatg
      301 cagtgccatt gctatccttg cctttttttg tacagtatta tccgatgact ttaaagcggt
      361 catcatcatt catgaattct ttttctcctg tttcagcttc gattgaacca aaatttagtt
      421 gtccacgaag tttccaacta gctggaacac cataaacagc ttgtactgat gcatcaataa
      481 ctggattgta atgttgtaaa ttaccaccaa gtccaagttc tgcagaaagg gctgtccaag
      541 tatttactga ggcaatacct gttgattgct cagaccaaac tggaaagtta tccgcataaa
      601 gaacgaattg ttcttgaaga gatttgacaa catcttgatc ttcaaagaag agtactgttc
      661 cctttgaagc tccaaaacta gctaattttt ccttagttcc agcaatagct gtctctggag
      721 caccttgtac tttcattgtt tctaccaaat cattagcaac caattcatcc cagaacttag
      781 taacttcatc attcaataaa atgacaacac gggaagtttg actattaaaa gcagatgggg
      841 attggcgaac tgcctcttta ataatctcta ctattttctc atcagaaact tctgtattgc
      901 gaccaagagc gtaaatagag cgacggtttt ttaattcttc caaaaatgac atattttttc
      961 tccttgttct tcaatctcag atatcttact acatttgttc tagtgacata tgaattagat
     1021 taaagttatt tattactatg tagtaattat attaccacta tatcgtaaag tcaaagaatg
     1081 tgactcacaa ggttttaaat ttttttaaaa ggttaatcaa gtcttcttgc tctgtttctg
     1141 ttaagactga aaaagcatct tctacacgtt taatatgctc tggtagagct ttttttatca
     1201 cttcttctcc ttcagttgtt aaactcacca aaaaggctct cttatcattc ggacaagaat
     1261 ggcgtaatac ccatcccttc ttttccatat ttttaataac aacagtcata ttacctgatg
     1321 tagcaagcat attttcaatc aattctccaa ttttcattgt gcctttagca tagagaacat
     1381 ccaaaactgc aaactgagtt ggtgttaatt caaattcttt aaatgattct gagacttttc
     1441 cgtctattgt tctaaatgct tttcgcatga caaccataga tttaaccgct gaatttttaa
     1501 agtttcccat ttcatctccc aaacgtattt tttcttattt tatcactaat tagttatttt
     1561 aagcaagaat atcatctttg ccctatcaaa agcagctggc aaataataag gttgagtaac
     1621 tactcaacct tattgcttag aagaaatcat caaaaagact caactgattg tcttctggta
     1681 aatttcccaa aatacccatc tcatcaaatt tttcaactaa ggtacttgag actcctcctc
     1741 gtttacgaag ctctgttttt gataaaaatt caccatcttc acgtgctcga actatctgtt
     1801 ttgcaacgtt ttctcccaag ccttccatag caacaaatgg agggattagc gtatcctctt
     1861 caataataaa gtcagtcgca tggctacgat aaagatctaa tttcccaaat ttaaagccac
     1921 gctcaagcat ttcattaacc aactctaatg tcgtaaaaag atcattttcg acattcgtcg
     1981 cctcattacg ctgacgcttc tcagtgatat ccttcatgcg cgccttaacg gcatcaagtc
     2041 cagcactcat tgtgcgtaat tcaaaagctt tggcgcgaat agaaaaataa gcacaataat
     2101 aaaatattgg ataatgtact ttaaagtacg ccacacgtaa ggccattaaa acataggctg
     2161 ctgcatgggc tttagggaac atatacttga tttttccaca cgattcaata taccaatccg
     2221 ggacattatt atcacgcatt gcttggatat aaccgttacg ctcatcttct gaaattttca
     2281 accataaccc cttacgcacg cgctccatga ttgtaaaggc catctttggt tgtagaccag
     2341 cgtgcattaa ataaaccatg atgtcatcac gacaaccaat aacggttgat aaggtagcaa
     2401 taccttcttt gattaagtct tgagcattac ctaaccaaac atccgtacca tgtgatagtc
     2461 ctgacagttg caacaattct gcaaatgtgg ttggatgggt ttcgttcacc ataccacgaa
     2521 caaagttcgt accaaactca ggaataccaa gcatccctgt aggagttcca atttgctctt
     2581 ctgtcactcc taaaacttcc gtaccagaaa ataacttcat aacgtctgga tcatctggaa
     2641 ggatatttga tggatcaatt cctgataaat cctgaagttt acgaatcata gtaggatcat
     2701 catgtcctag aatatctaac ttcaagacat tctcatcaat atcatggaaa ttgaagtgag
     2761 ttgtctgcca agcagctgtc atatcatctg ctggatattg aaccggagta aaatcataaa
     2821 catccatata gttaggaata acaacgatac caccggggtg ttgacctgta gaacgtttga
     2881 ctcccgcagc accagtcgct aaccgttcga cctctgcatc gttataaaat ttattgtaat
     2941 ctcgttcgta acctttgaca aaaccaaagg cagttttttc agctacggta cctaccgttc
     3001 ctgcacgaaa agcatattct tctccaaaga tatcacgaac atctaagtga gcagaaggtt
     3061 gatcatctcc agagaaattt agatcaatat caggaacctt atctccatca aatcctaaga
     3121 aagtttcaaa cggaatatcc tgtccatctt tcttgtacaa agtaccacat ttaggacaat
     3181 ttttatttgg taaatcgtaa cctgaaccac aagaaccatc tgtgataaat tcagaatgct
     3241 gacaatttgg gcaaacataa tgaggtggca ttggattaac ttcagtaatt cctatcatgg
     3301 tcgctacaaa actcgaccca acagacccac gtgatcctac tagataacca cgttcattag
     3361 aacgttgcac caacatttgc gaagcaagat agataacggc aaatccatta ccaagtatag
     3421 atgctaactc tttttcgata cgtaaatcaa taatatcagg aagaggatta ccatatattt
     3481 cgaatgcttt agcataagtt aattcagcaa ctcgttcttc agcacgatca acaaacgggg
     3541 tatagagatc cccttttaca acttcaacgt cttcaaatcg atctgcaaaa gtattcgtat
     3601 tggttacaac gatctcatag gctaaatctt taccaagaaa ggcaaattca tctaacatct
     3661 catttgttgt acggaaatgt gcttttggca aaggcgctgg ttgagcatcc tccccacgac
     3721 cgatagtcct attaatcatg gcaccttgtc caagactacg aacaataatc tcacggtaaa
     3781 tctcatcttc aggctcaata taatgaacat ttcccgtagc taaaacaggc ttatctaaac
     3841 gccgaccgac ctctattagg tctctaataa tttgttgaat ccccgcctca tctttaatta
     3901 aatcacgaac caccaagggg cgataaatag caggtggcat gacttcaata aaatcataat
     3961 atttagctag ggtcactgct gcatcaattc catttgataa caaggcatca aaaacttctc
     4021 catcagaaca ggcagtacct agcaagagac cttctctgtg agcatctaaa actgatctag
     4081 gaattcttgc caccccttca aaatatttaa catttgaaag gctaactaac ttaaatatat
     4141 ttttaagtcc aacctgattt tggacataaa tggttgcatg ctttatgcgt gcctttttgt
     4201 aagagtcttc agctaccaat ttagtattga gctccatcaa attagtcaca tcacgatttt
     4261 cccgcgcctc ttttaagaaa ataaagagta agcgacccgt tgcttcagca tcgtagttgg
     4321 ccatatgatg atgttccaaa gcaacttgga aacgtttggt tagaggcccc aaaccatgac
     4381 gcttatattc aggataaaga tttcttgcaa attctaaggt atcaatgacc ggctgtgtga
     4441 tcaaaggcag attatgacgt tcataattcg cattcataaa accaacgtca aaggttgcat
     4501 tatgcgcaac taaaaccgta ccttgacaaa aattctgaaa ttcctgaagg acttgtaaaa
     4561 taggttttga acctctgaca tgattatccg taatacctgt caactcagtt gtaaagtcag
     4621 aaagaggatg gcctgggtca ataaactcat caaattgctc aataatatta cccttgaaca
     4681 tttttgatgc tgcaatctga attaggtcat tattagctgc agaaagacct gtagtttcca
     4741 catcaaatac aacataagtt gcttcgtgca aattcatatc aacttcgttg taggaaatag
     4801 gcactttatc ttcaacaata tttgcctcta atccaaaaat tgcttttata cctgcttttt
     4861 ttgctctgtg gtagccgtgc ggaaaacttt gaacattggc atggtcagta attgcaatag
     4921 ctgggtgtcc ccacttagct gcagtatcaa tgaggctctc gacagttggt aaagcatcca
     4981 tcgttgacat attggtatga gcatgaaatt ccacacgttt ttgatctgct ggcattaagt
     5041 ctttacgttc atgatgcaca atctctttga tatcttgaac attcattgtc aaagatttag
     5101 taaagttatt gttctcaata ttccctctga cccgcaacca cgaaccttta gaaatcatat
     5161 cgtacttctt caattcttca tcatctttag cccacttttg catagcaaaa cttgaggtat
     5221 aatcagtcat cttaaaatta atgatatgac gtccagtgcg ggttgttttt cgttcaacac
     5281 taaaaaccat tccttcaaaa acgatacgat tttcttctgt ggtcacctca atcatgggtg
     5341 taatctctgc tttttcaaag ccagcttgac gttgtttgat acgctctttg aaatcataat
     5401 tttgagttgg tgtcacttct tctgatggag gcgctgaatc ttctagtgat ttttgtgctt
     5461 ccaaggcttg catagcctct tggttagctt tttcaagcaa ttgctcgcgg ttagtttcaa
     5521 atgatgattt taagttttgc gtcatttgtt catcagaaac catatcaata gcaagtttac
     5581 caaatccaaa caatgaaaat tgttgttcca agcgcggcaa atgattctga cgaaaatgat
     5641 tattattgac aaattgaggt gctgaaataa tcatctgact gccattgtaa tgaactttta
     5701 attgtgaaaa agaactctta aaacttgcac tattacacaa gggttcacaa aaagcttgtt
     5761 gataataatc ctgtagcaga tcatcagaaa aatcaatagt ttctgctctt atatcaaaag
     5821 tagcctttat atctgctttt tcaaaactat tcactaaacg agtttgtaat tcacaataca
     5881 cctcaattgg caataattct ggaaatgaaa aatgaaattc ccataagcgt gatagagaat
     5941 gaacctttac ctcgataata tcagcagacg aaaaaacact tgaattctta atttcaagcg
     6001 gcatttcaat ctgatccata agtttcttga acaattctga catagttttc tcctgaatat
     6061 cttacttcca ttttatcaca tatctgtaaa tatttcgaga taacaaaagg aggctgttaa
     6121 aaaaggcatt tattagcctt cttaacaaca ctctcttaac tgagactatt tatcataagc
     6181 atctagactg taggtttcaa taatttggtt taatttacta gcataagcag tatctgtcgc
     6241 ataaagacct gttagagctg cagtagcatc cttataagaa gaagtatttg atttccaaac
     6301 agatgcatac ttttgactag atactaactc agcataatca tatagtgaag cagaataatt
     6361 tggataggcg cgaaaaggag cttggatttg agtcatattg cctttcccat catcttctaa
     6421 agtaggcatt tggacagatt tacctttata ttctcctttg atgccaaaga ggttataatt
     6481 aggagcctta gacaaatctg attgtccact ggatgattcc aaaatagctt gtgctaataa
     6541 gactgacgca tacaaatcat aggtcttaga aatttcaata gctgttggag aaatcgtttg
     6601 aataaaagtc gtcgtttgat ccgatgcact agtgactgtc ggttcgacat tatcattttt
     6661 ataattgtaa aaccaaaccc cacccaaacc aaaaactaat agtgttgttg ttaacctcaa
     6721 taccaattta tctttttttc gacttttcat gatggctcct ttctaaaata tcatttcatt
     6781 ataacaaaag gcagttgctt tttaccaata agaaccatac taaatttcac tccatataaa
     6841 aaagttagca catcagacta acttttttat cggcttattt ttttgttaga atctctaaag
     6901 tttcaattaa gttatcagcg tgaacctcaa tcgtatcacc acttgcttta attttcactt
     6961 caacgatacc ttcagaagct ttcttcccaa cagttactcg aattggaagt ccaattaagt
     7021 cactgtctga gaatttagaa ccaacacgtt cattacgatc gtctgtcagg acttcataac
     7081 ctttaagcat caaatcagct tcaattttct cagtaagatc ttgtgactct tgatctttga
     7141 cattgacagt aatcaaatgt acatcaaatg gggctaattc ttctgggaaa ttaatccccc
     7201 aagcaaagcg ataagctcct tttggtgtct tattcacaaa aagtcgggca tgttgttcaa
     7261 tgacagctga gagaatacga ctaacaccaa taccataaca ccccataaca ataggatttg
     7321 agcggccatt ttcatcaagg atgtttgctc ccatactatc tgaataacgg gtacctagtt
     7381 taaagatatg tccaatttca ataccacgag caaatttcag agtaccttta ccgtctggag
     7441 aaatctcccc ttcttttact tcacgaatat caacgtattc agctgtgaaa tcacgttctg
     7501 gattaactcc agtaaaatga taaccatctt ggttagcacc agacacagca ttagcaaggt
     7561 cttgaacttt acgatcagca ataattttaa cagactcagg taggttaact ggcccaagag
     7621 aaccaaaacc tgcaccaaaa atctcttttg cttgctcttc acttgcaggc tctaagaaat
     7681 cagccgctaa ataatttttt aacttcacat cattgacttg atcgttacca actagtaaag
     7741 caaccacggg ctgttcatca gcaataaaca atagtgtctt gatagtttga ttttcatcaa
     7801 ttgataaaaa tccagctacc tcatcaattg atttgcagtt aggagtttct acttttgtta
     7861 cttcttcaaa ggtagcagcc ttagtagatg gtttgtactc attcgttgcc atctcaaggt
     7921 tagcagcata gcttgattca gtggagtatg caatagtgtc ctcaccagaa actagccaag
     7981 ccgagagttc tgccttaatt tcttctaaaa catcctctgg aatatcatca atagaaggaa
     8041 tagttttatc aaggactaac caacgattta aatctgtacg attgggagtt actgccataa
     8101 attcttggct atctttacct cccatagcac ctccgtcacc tataatgcct ttaaagtcaa
     8161 gtccagcacg tgtgaagatg gcttcatatg ctttacggta atcttcgtaa gtgacatcaa
     8221 ggtcttcata atctttgtgg aagctgtatc catctttcat gataaattca cgtgttcgaa
     8281 gaagtccatt acgaggacgc ttctcatcac gatatttaga ctgaatttga taaaggttta
     8341 gtggtaattg cttataagat tttactgcat cacgaacaag agttgtgaaa gtctcttcat
     8401 gagttggtcc taagataaaa tccgattgat cacgattctt taatttataa aggtcttcac
     8461 catatgtttc gtagcgccca gattcacgcc ataaatctgc tgtaagaaga gctggagcta
     8521 acatttcaac tgcaccaatc ttctcaaatt cttgacgcat aattgtttta aatttttcaa
     8581 tagtacggtt agctagaggc aaataagcat agattccagc cgaaacttgg cggacatatc
     8641 ctgcacgaac cataagagca tggctgataa cctgagcatc acttggcatt tcgcgaagtg
     8701 ttggaataag cattttactt tgtttcatta caataagata cgactaatca aagttgattc
     8761 tttttcaatt gagaattgca attgaaaaat tcgtatttcc tttcacgttt tattatcttt
     8821 tagaagaaag ctcgcatgat atcgttccag gttacagcaa tcattaatac taacataact
     8881 gctaccccag cgagcgtaat ataagtttct gtttcttgtt taagtggttt gcgacgaatg
     8941 gcttctaaaa tattcatcac tatcttaccc ccgtcaaggg ctggtatagg gatgagattc
     9001 ataataccta agttgataga gattaatccc attaagttga gaactgtgac gaaaccattt
     9061 ttagcagctt ggctactagc ctgatagaga gctactggac ctcccaattt attaatacta
     9121 aaatgagcaa taagtttctt taattcattt aaaattctaa agaaactttc ccatgctagt
     9181 ttaaatccac ctaaaagctt gtctttaaag cttgtcttta gagctggcat aatgccgatg
     9241 atgtaggatt tatcaacttt ctgtggctta acgttgatcg ttttcaccac ctctttgctc
     9301 ttaattttaa gggcaagttt ttggtttttt tctaaatgac tagttgactt ttctactgct
     9361 gctgtcaact gttcccagtt agaaactttg tgcgacccta tttgtaaaat acggtcatta
     9421 ttttttaaac ctgcactagc agcaggacca ttttctgata cacggacctg attggttgat
     9481 aaatcttgaa cacctccttg aataaaagca agtgcaataa agactacaag acctaaaata
     9541 aaattattca tcggacctgc aaaattggta attaagcgcc cccatacaga agcattctga
     9601 tactgcacat ctaagggagc aatacgaatt tcagtaccat cctcctcaat gatagtagca
     9661 tcgtgatcaa cactataggt ttttgtctca cttaagacca atcctgtgat agttagttta
     9721 tcttctaaat cataagctgt tacattgatt ggtaaagaag tattgtctaa ttgttttcct
     9781 gataaattaa tacgagttac aatgccttct ttatttaaag taaggctagc cggcgtccct
     9841 gtcttaattt cagtcttatc atcaccccaa ccagccatac ggacataacc acctagcggt
     9901 aatatacgaa tagtataggt tgttccttct ttatcaatgt gagaaaagat ttttggaccc
     9961 ataccaatag caaattcacg aacaagaatt cctgatttct ttgcaaaata gaaatgccca
    10021 aattcgtgaa caactacaat gacaccaaaa ataataataa aggttaaaat tcctagcatt
    10081 caattcctct ctaaatacaa tatattattt tagaataagc caaagaagtg catgattggg
    10141 aaaacaaaaa tcatactatc aaagcgatcc aaaatccccc catgacccgg aatcaattta
    10201 cccgaatctt taacaccaaa atgacgttta atactacttt caactaaatc tccaaattga
    10261 ccaaaaatgc taaagatagc cactaaaact aacatgacta ggaagctatg aggcgcatag
    10321 acggtcttat caaaaagcat aaaaaagaaa gctaccacaa tggctgaagc aattcctccc
    10381 agactccctt caattgtttt atttggagac actgaaggta gtagtttgcg ttgtccaaat
    10441 tgacgaccaa tcatataagc accgatatca gtcgcccaga caataaataa agctaacaac
    10501 accttatcta ttccagccat acgagcactg actagatttt gaaagccaat acccacataa
    10561 aaactagacg caattggaaa ggcagcatct tcaaaactgt aagaattact gttcaaaaca
    10621 gtacctgcca aaatcataaa aataacaata ccgtatgctg ataaactagc gtcaactggt
    10681 aaaaaggaca aataactatc taaaggaact gttaaaacaa aggctcctat catagctaat
    10741 gcaccttcaa aggaaaatat ttctaaacga cgcatacgaa gtaattcaga aacaccaatc
    10801 atagctaaaa gtccaactaa aaattggaaa ggcaaaccac ccattactaa aaaagggata
    10861 aatatggcaa gcgcgacagc tccccaaata acacgttctt tcattttctc tcttctttct
    10921 atacactacc aaatcgacga tggcgttgat tataatccac aatggcctta tgtagttcgt
    10981 cttttttaaa atctggccat aaaacgggag taaagtaaaa ttcactataa gctgattgcc
    11041 aaggtaaaaa gttactcaag cgtaactctc cactagtgcg gataatcaaa tcagggtccc
    11101 tataaagata gggtaagcta tttgtcatta aatgatttgc tactaaatcc tctgtaatat
    11161 cgtcaggatt caacttagct tccaaaacat cctgagcaat ttccttaatg gcattagtga
    11221 tttctgaacg tccgccataa tttagcgcaa aattcaatac gagccccgaa ttatgttttg
    11281 tcctcaaaca agcgcgttgc attgcatcgt atgttgcctt aggtaattta tgagtatctc
    11341 cgatgacctg tacacgtaca ttatttttat ctaactctgg tacatattta tcaaaaaatt
    11401 caaccggaag gttcataata aacttaactt catcatctgg acgtgaccaa ttttctgtag
    11461 aaaaggcata gacagttagt actttaacac ctaattctga agctgctatt gtaacttctt
    11521 gaagagcatc catccccgcc ttatgtccca taacgcgtgg ttttaaacgt ttttttgccc
    11581 aacgtccgtt accatccatg ataataccaa tatgtgctgg tattttctcc aaaggcattt
    11641 ctacttgttt ttttctttta aatataaaca taatgttcct taatttagca atcgtttttc
    11701 ttatcagccc ttttaaaaga gctggaaata tcccatacta taacatttta ccacatttta
    11761 acttattttt gagtattaaa atgccatttg tgacagcaaa ctaaaaagac cagaacaatc
    11821 atctggtcct acatatctta ttcagtttcg atggctgttt ctttattttc gtcttcattg
    11881 acttcaataa tcttaacatc ttcaactggt tctgttggtg ttgctgcctt agagacaaca
    11941 gatttgatag ctgccaactc aaacgtcaag tagacaccat caacatctaa aaccattcgt
    12001 tgtgcttcag tattaacttc atcaactact ccaaaaagac caccaattgt gacaatttca
    12061 tcaccttttt gaacagcatt caattgtttt tgacgttctt gtgcttgttt cttttgctga
    12121 cgttgcatga agaacatcat accaaccata acaacaagca taataatagg taagctcatt
    12181 tgattctcct ttccgtgttc tacgtaatca ttactatagc agatttatgc tatatctgca
    12241 aggagatact gtctcttagc caataaattc acggatttct tcttttgtca tttttgaatc
    12301 gcacttcacc ttgatagttc cgtttttaac aaccatcaaa cctggcactg taggaatgtc
    12361 atatttattt cgaaaatctt tatttgcagt ccaatgatca aaatcttcac tatctaaatg
    12421 gaataattca aattcatctt catcagctac ttctacaatt ttaggaacaa agaggtgaca
    12481 aaatggacaa gaaatacgac caatgaacag tacaaaatct tgatttgaat caattttatc
    12541 ttcaatttcg gcagccgtta tcaaagtcat ggttgccaaa agttcttcaa aagaatacat
    12601 agttaaccaa ctttctaaat cttatattta aagaataacc tatgatggca aaacttgcaa
    12661 ggcaagagta acaacgattc tatcaaaatc attaactcac ttctcgcttc aaactatcct
    12721 caaaaatgct cactcatatt taaatgactc atcaaaaaaa ctgagcatag ctcagttttt
    12781 attttacacc ttatttttgt acagcattac taacgctttt agcaactaca tcggccacat
    12841 cattttgaaa agcatttgga atgatgtgag ttgtgcttaa ttcttcctcc ggtattaaac
    12901 tagctatgcc tctcgctgcc gcaatttgca tttcaacagt tatcgttttt gctctagcat
    12961 ctaatgctcc tctgaaaata cccggaaatg ctaaaacatt attaatttga tttggaaaat
    13021 cactacgacc agttccaact atgtaagctc cggcttttaa ggcttgatct ggataaattt
    13081 ctggaatagg attcgccatt gcaaatacaa taggtttatc agccatcttg gaaatccatt
    13141 ctgcatgtaa tgcttccgga gcagaaacac ctataaaaac atcggcattc tccaaagcat
    13201 cttctaagga tccagattgg aattctctat tagtcaattt tgcaatcgct ttgtgatgag
    13261 gggctaatga ctcccgatct ttatcgttaa taatgccaaa tcgatcaact accgttacat
    13321 gttttgctcc tgctgataga agcttccttg ttatagataa gcccgctgaa cctccaccat
    13381 taacaactac tttgatatct tcaatgtctt ttttaactaa cttcaaactg ttaaacaaag
    13441 ccgctaatac tacgattgct gtaccatgtt ggtcatcatg gaaaactggt atatcacatt
    13501 cttcaattag gcgttgttca atttcgaaac aacgtggtgc tgaaatatcc tctaaattaa
    13561 tccctccaaa agtcggcgaa atcgctttta ctatagaaat aatttcctct gtatcatttg
    13621 ttttcaacac aattggcaca gcatctacat ttgcaaaacg tttaaagagt gctgctttac
    13681 cttccatgac tggcattgct gcctcaggac caatatctcc taatccaaga acagctgaac
    13741 catcacttac tacggctact gtattttttt tagtcgtcaa ctcatatgca agctccacat
    13801 cctctgcaat agccgatgat actgacgcca cccctggggt ataggcaata ctaagatccc
    13861 tcttatcttc caccttaact tttgaaatta cttctaattt tccaccattt tctctagctt
    13921 ggttgatagc taattgacct aaattttcac tcattttgat aactcctttt gataaatatt
    13981 agaataacat gcgtaaaatg gctgtcatcg tgataacagt aatagctcct cctaaacgcg
    14041 tagcaacttg agcaaatggc atcaagttca ttcggtctgc tgtacttaaa atggcaacat
    14101 ctcctgttcc tcccatacca ctttgacaag ctgaaataat acctgcttca acaggattca
    14161 tattaaggaa tcgcgataca aagaatccaa cagaaataac tgtaaataca acactaataa
    14221 caactatgaa atattgtatg ctaagcgttg ccacaacatc ttttaacggg atataaagca
    14281 atcctagccc tgccattaga ggaaatgtaa aattaccaga aataaattta taaagttgct
    14341 tagctccatt ttgtgtttct ctaggaataa cattgatata tttcaaaata gctgctgcga
    14401 taatcattaa tacgggacca ggaaatccag ttaattgctg caacaaatgt ccaaaaataa
    14461 agagactaca tgctgttaaa acacctccac ccattttctt aacgtcaatt gggccagaat
    14521 gatcagcaat aatatctgac atgtcctctc ctttatttaa ccttactaat tgcccttgac
    14581 cagacaagtg tggtttcttt tctcccaaac gattcaatag tgcagtacat aaaatggcaa
    14641 agaaattacc aataatagtg gctgggatga gttgagcaac tagttgttcg ctagctacac
    14701 cggtaattga actatagcct aacgataatg gtaaaatacc ttcaccaata ccaccagcta
    14761 aaacgggtgt tacgatataa aacaaagtgt gtcgccactc taagcctaaa ataacaccga
    14821 caaatgtccc taccatcatc gcacaaacca ttcctaataa catggggaaa atcattctta
    14881 gtaatccctg aatcaacatt ttccgattca tccctaaaat actaccggac actaaacaag
    14941 caatataaaa ataaagaaag ttagcttgct tcatcaacat atttgttgac tctaaaacat
    15001 ttttattaat gaggttgaaa aacaccaaaa tagatggtac taataaagat aaaatagctg
    15061 ggccaccgaa gtttttaaaa ccagggatgc tagctccaat agtacctaag aaccatccca
    15121 ttgttaagat gactgcaaat cctcctagca tattgactgg caatttttgt aaaaagcctg
    15181 ctagaagaat caataatgct aaacaaacat atactggtaa gggaactgag cctaccttaa
    15241 tatgaaatag tttagtcttt aagtcttgct tttgacctct agaaccctca ttattcacta
    15301 ccttcacgtc tgccatgaaa gcacctcctt ttacttctta tgtacagttt actttgggac
    15361 ttagtgataa ggaagtttat ttaagtaatt gaattatttc aacttattta attaatttaa
    15421 gctggattct agtttttagg ctatgttata ctaaactcgg aggatgctga tgaaaaaaaa
    15481 actatctctt tgggcatttc tatcattgat tttagtcact atgaccattt gtatattttc
    15541 tattttttat tatgtcacta tacatcagtc ctatcgtatg gtaagggtac aggaagaaaa
    15601 aatcttaaaa aatactggtt atgctttatc gagaaatcca caagttatac aaacactcaa
    15661 agataatcac tataatcaaa gtctccaaaa acaaatgcta ttccttagca aaaaatcaaa
    15721 tttagactat attgtcctta taaatttaaa agggatccgt tttacccacc ctgactccac
    15781 taagattggt aaaccatttc aaggcggtga tgaacaagct gtttttaagg gaaaagctat
    15841 tatgtctact gcagaaggtt ctttaggaaa atctttacga tacttaattc cagtatatga
    15901 tcaccaaaaa caggttgggg caattgcagt tggcttaaaa ttaacgacac ttggagactt
    15961 atctcaaagc agcataaaag agtttagtaa gcctctcttg attagtattt tgattagctt
    16021 agtcgtcaca agtataattt cttatggctt aaaaaaacaa cttcacaacc tccatccaag
    16081 tgatattttt caacatttag aagagcgcaa tgcaacactg gaccaaattc aagccgctgt
    16141 atttgttatt gaccagaggc atatcatcaa aagaaataat cctgcagcta gtttattatt
    16201 caaaaaagag gggcaacgag acctattttc aggtaaatta ttagaatctt taattcctca
    16261 actcaagcag gatcattttt caaaaaaaac tgaacaagta ttacattttc aaggacaaga
    16321 ctatttacta tctatctcgc caattacggt taaaacacaa aatagaggct atgttgtctt
    16381 cttaaggaat gttactgaaa cacttttcac tttggatcaa ttagcacaca ctacagctta
    16441 tgcttctgct ctacaggccc aaacacatca atttatgaat caacttcatg ttatttatgg
    16501 acttgcagac atcgaatact acgacgagtt gaaaatttat ttaaaagaat tattggagcc
    16561 acaaaatgaa tttttagcac gcttatcaat gttagttaga gagcctcgac tggctagttt
    16621 cattattggc gaaagagaaa aattcgcaga aaaacacatc aatctatcca cagaaatttt
    16681 agtagaaatc cctaccaagt caactgttga ggatgttaat aactatctct tgctccacag
    16741 atatattaat acgaagatac tcacgctatt aaattcaacg actttagttt ctttacgttt
    16801 aaattatcaa aataacctca ttgaaacaga ttatcaatgg gagaatgaga agtggttact
    16861 caatgactac catcagtatt tcaacgacgc ttattttcaa cagttacttg tagattcaag
    16921 agctacttat aagatctctt taaaacaaaa taaggttatt ataagaacaa atattatcta
    16981 ctcagaaggg tcttagtatg gacgtattaa ttatcgaaga tgatcctatg gtggaattta
    17041 tccaccgcaa ttaccttgaa aaactcaatt attttcaaaa catctattca actgctagtc
    17101 aaacacaagc tattgcttat cttaatgaca ttaaaatcca acttgttctt ttagatattc
    17161 atattaaaga aggcaatggt ctagaattat taaagttatt acgcaatcaa caccaaaata
    17221 ccgaagtcat tgttatttca gcagccaacg aggcgcacac tgtcaaagaa gcatttcatt
    17281 taggtattgt agattacctg ataaaaccat ttacatttga acgctttgag tctagtattg
    17341 aaaaattcct aaatcattat catacatttg aagctgataa aatctatcaa gacaacatcg
    17401 atcactttca aaaaatagat tctggatggc tagagggaga ggttaaactt gatgagaaag
    17461 ggctctctga aattacctat cagcatattc ttgatgccat tcaagagtta gagcagcctt
    17521 tcactatcca agagttagca aaatgcagtc aattttctca tgtctctgtt aggaaatata
    17581 ttgcctatat ggaagaaaaa ggccttctga caagtcagca aatttatact aaggtcggtc
    17641 gtccttacaa agtttataag cttataaaaa aaacttaggc ctgtagacct aagttttttt
    17701 aatcttcgta gccatttgga tgactagaat gccatgccca tgccgatgaa ataattttat
    17761 caatattatc aaatttaggc tcccacccaa gcatttgacg agctttctct gaggaagcaa
    17821 taagcgtatc tggatctcct ggacgacgag ctgccttttg agcaggaatt tctttcccag
    17881 taatacgacg agccgcctct aacatctgaa ggttagaaaa acctgtcgaa gatccgagat
    17941 taaacacgtt tgattcattc ccttggcgaa ggtaatcaac tgctaatata tgagcatctg
    18001 ccaaatcaaa tggatgcaca taatctcgaa cattagttcc atctggagta ttgtaatcat
    18061 ctccaaaaat cattattttg tcacgtactc cttgagcaac ttgaagaatg attggtaaca
    18121 aatgtgtttc tggtttatga tcttccccaa ttgagccatc cggtttatct ccagcaacgt
    18181 tgaagtaacg tagagcgaca aacttaatac cgtaggcttg atcagcccat ttcataattg
    18241 tttccatcat aagcttactt tcgccataag gattaatagg attttgagga gctgtttcta
    18301 aaataggaac ttgttctgga ataccatatg ttgcagctgt tgatgaaaag acaatatttt
    18361 tgatatcaca ttcattcatt acttctaaaa gttttatcat tccagcagtg ttattatcaa
    18421 aatattttaa aggattttcc attgactcag caactagtga aaatgccgca aaatggataa
    18481 cagcatctac atcaggattt tccctaaaaa cttgacgcat aaacgtttta tcggataaat
    18541 ctccttcata aaaaatcgca tctgaatgca ccgcttgacg atgacctgtt accaagttat
    18601 caacaacaat aactttctct tttccttgtg tgattaactg atcaaccata tgcgagccaa
    18661 tgtaaccggc gccacctaaa atcagaactg ccatattttt ctcctatttc aatactcttt
    18721 taacatcatt tgttacctca ttatactgag aataagcttt ttttgcaaat atctaccgaa
    18781 attagaatag ctctatgcag aaggcatccc acggcttgag agtgttactt gatagtactt
    18841 cttgaacctc tgtatttgaa ataatagttt ctttaacagc ttctggaaat tcaaaagatt
    18901 ggttttgatc ggataagttt gcaactatta aataacgttc tttatctgtt tgacgaacat
    18961 aagcaaatac cttatctgct gtttccaaaa gtttaaaatc agcatcaact aaccagtcgt
    19021 gttctttacg taaagcaact aatttttgat aagtataaaa aatactttcc gtatcattta
    19081 acgctgcctc cacattaatt tcctgataat tgggattgac tgctagccat ggggtaccgt
    19141 tagtaaaacc agcttgatgt ccttgactcc actgcattgg agtacgagca ttatcacgac
    19201 cgactttacg aatactatct aaaattgttg cttcagatac cccattatcc atggcttctt
    19261 tagcatagtt aagggattca atatcatcta catctgctaa acactcaaat ggataattgg
    19321 tcataccaat ttcttcacct tgatagatgt aaggcgttcc acgcatcaaa tgtagtagga
    19381 ttgctagagc cttggcagat tgttttctgt tatcactatc atttccccaa atcgagagca
    19441 cccgaggaag gtcgtgatta ttccagaata aagaattcca accttggcca agctccaatt
    19501 gtgtttgcca ttttgtaaaa atatctttca atgctggaac atctaatccg tcactgtaat
    19561 cccacttagg agcgtcaggt ttatgttgaa gacctacatg ttcaaattga aagaccatag
    19621 ataattcttc attatcggga tttgagtatt gttttgcaat gtctggcgtt gctccccaag
    19681 tttcacccac cgtcaataag tcatgctttc caaaactagc tctgttcatt tctttaagat
    19741 aatcatggag ttttggtcca ttaccagtaa tgcccttatc tggaattttc ccaattaaat
    19801 caatgacatc catacggaaa ccaccaatac ccttatcaat ccagaaattc atcatatcat
    19861 aaattttatg acgtaaagct tcattctccc agttgagatc tggctgacgt ttgctaaaaa
    19921 ggtgcaaata atattgacct gatgctttat catattccca agctgagcca ctaaaaatag
    19981 aagtcaaatc gttaggttca tctcgccaaa tataaaaatc acgttcagga ctattagggt
    20041 tttctcgcgc ttctacgaac caagcatgtt catcagaggt atgattcact accaaatcca
    20101 tgatgatttt aatcccgcgt tgatttgcct cttgtaacag ttggtccata tcattcatgt
    20161 caccgaaaat atctgcaatg gcttgataat ctgaaatatc ataaccattg tcatccattg
    20221 gactttggta aacaggtgat aaccagatag cagtaatccc taatttttca aggtagtcta
    20281 gtttgctagt aatcccttta atgtccccca caccatcacc atctgaatcc ataaatgaac
    20341 gtgggtaaat ttgataaatc gtagctttat gccaccagtg tttcttcatt attcttctcc
    20401 tatcaataat tcattcatct tttattttaa cgtttttcct cttttagtgc aagcgtttgc
    20461 gcaaataaca ttataaaaag gctggaaccc aaccttttta tgaatatcgc ttatacctta
    20521 acgaatagct tgttcagtat ctgcatcaaa gaaatgcccc ttagcaacat taaacgttag
    20581 gttaactttt tctcctggat tatggaagtc acgcgcttca acacgagaag caaattctgt
    20641 ttgacctaac ttaacataaa gcatagtttc gctaccgagt aattcagaaa ctaaaacttc
    20701 tgcttcaaca ttggcatttg gataagtatc ttggacaaga agattgcttg aaatgtcttc
    20761 tggacgaata ccaaaaatca attcttttcc tttatagcct cgtgattcta aaagtttttc
    20821 ttgaccttca gggatagcga ttcgaagacc atcttcacta ataatcatac cattttcaac
    20881 tttaactttg aagaagttca tagatggact accgatgaat cccgcaacaa atttattagc
    20941 aggaagatta tagagttctt gtggtgaacc aacttgctca attttaccaa ttgtaccatc
    21001 accatcaggg ttcttagtag ctgacataat gacaatacgg tctgccaaag tcatcgcctc
    21061 tgtttggtcg tgggtaacat aaatagttgt tgaaccaata cgttgatgaa tttttgcaat
    21121 ttcagcacgc attgaaacac gtaatttagc atctaagtta gataaaggct catccattaa
    21181 gaacactttg gcatcacgca caatagcacg tcccatagca acacgttggc gttgtccacc
    21241 tgacaaatcc gcaggtttgc gctctaagaa ttcagtaagg ccaatattag ctgctgcttc
    21301 acgaacgcgc ttatcaattt cttgttttga gaatttacgc aatttgagcc caaaagccat
    21361 attatcgtaa acagtcatat gtggataaag agcatagttt tggaaaacca tagcaatatc
    21421 acgatcttta ggtgatttgt cattgacaac ttcgccatca atcttgagct caccttcact
    21481 aatatcttca agacctgcga tcatacgaag tgttgttgat ttcccacaac ctgatggtcc
    21541 aacaaaaaca ataaattctt tgtctttaat atccaagtca aagtcttcaa cagaataatg
    21601 cgatgcactt ggatattttt tgtaaatatg atttaaattt aattctacca ttagattgac
    21661 ctcttttatt attgtgattt tattatagca aagaaagggt tttcatataa tggtaacctt
    21721 gcctaaattt cttgtgcaaa ctaactaaat tttaggtatc catcgaaatt aaataggcat
    21781 aggctaaatc atctaatatt tttaaattaa gccccgaaga ttgggtaaat ttatctaatt
    21841 tatattgcaa tgaattccga tgaatgaaaa gtgccttagc cgttttagca aggttccctt
    21901 gctcttgcca caaggctttt attatagccg aagtatcact cgtatcaagg atatgttgta
    21961 aacattttgc tttaatagaa ggcatgggcg attgatttgc caaagcccat aaagccatct
    22021 ttggaaatcg acagtaatgt tgttcggcct tgtgactaag aaaaagcgta aaaagtttat
    22081 attcttcttc atataattct ttccagtcca ctgcttgtag atgcgtccaa ctatttccaa
    22141 aatagcatct taaacgcgtg ttaaagtcat tttcaatcgt tggtaaaata tctgttagta
    22201 attccgtagc attaaagaaa ttatcctgat ttaaaatcat gacactttgg gtttgattaa
    22261 ttgtcataat tgtttctacg tttattacta aaggcgacaa gaaatcctta agttcttctg
    22321 ataaggtaaa ttgatgctca ataaaaataa attggactgc cgaatgagat tggggcaacc
    22381 ttccacgacc ttcaactaga tagcggtacc aaggattctg agaatccact gaactcgcct
    22441 cttccaggct ttgtgtcatt tgtaataagc atagttcacg cttagttagc gaagattttg
    22501 gaaagtggag gacatgacca tcttgctcaa cgatgatata atcctcactc tgtttaactt
    22561 cctgtttagt aataacttct ggaaacagtt ccttaaaatc catggcatcc tccactacag
    22621 ttttgttcat tatatcatat ttataacctc tttttgagca cagaaaaaga ggagaatgtc
    22681 tcctcagaaa gtttctgtta taaaatagtg atccgaatag aatgctgttt cgactgcttt
    22741 ggattaagcc gtacgatatt ttgtttatct tctaaaatat cagactcttc aatagaagtt
    22801 gacaaggaac tccagggttc caaagccaaa aaaggaccac cattgttact tgtccaaaga
    22861 ataagattct caaaatcatc aaagtcaagt tggattcctt ttgcatgatt tctagatttt
    22921 aagtagaccg tcttagatcg tagttggtct aaagtaatag catctttttc aaataaagca
    22981 taattaagag ataattgttt ttgattttct aaaaaaggat gtctagcttg taaatctaac
    23041 aagcctgtat ccggaaacga ctcaggaatt gtgcaagttt caaactgttc aaactctaga
    23101 tagtaatcac taaatacttc cttttcaaac aaagggcaat taaagcctgg gtgagctccg
    23161 atagcatatg gcatattttt ttctgattct aagtttttga caatatatgt aatggcaatg
    23221 cttttatttc ttaaggtgta agttatttct aaactaaaac gataaggata atttatcaac
    23281 gtttcttcac tacttttaat tgtaaaacga acagaagaat ccgatatgta atcatagtcg
    23341 aatgttttgt gacgtacaaa gccatgcctt ggtatgatac ccgtaaaatg tggagcttga
    23401 gctggtctgt aaatgacgca atcatttcga acacttccgc atataggaaa taaaatagga
    23461 gcttgcccac cccaataagt gggttcccct tgccaaaggt actcaacgcc ttgactatct
    23521 gtaatagaag tcattgttgc acctaacgct tttaatgtca cttgcaactc atgattttgt
    23581 atcgtaatag ccattatttt tcctttcata aagagcaata attgctttac caacttttct
    23641 ctagcaatta tctttccttc caagaagttg ctgttttttg caaaatttta tttagttcat
    23701 caatattttg aaaacctatt gtacgtaacc attgacgtgc accagcttct cctttttcaa
    23761 catactcttt aacacttcca gcccaagttg ctctaccaca aaggacaccg ttaaatttag
    23821 caccagcttc tttagcaaac acaagagtct cttgaaaaag ctgagcagga actcctgcac
    23881 tcaaaaaaat gtaggggagt agtgtcgcct gatcttgttc tctaaaataa gccgcagctg
    23941 tcgctttatt ataagcagtc tctccttgcg caaaaccttc tacatagtcc atattgacag
    24001 gcacttccac cttcaagaca tcaatattaa atcgaggatt tgaaaaaact ttcatagctt
    24061 caataacctt acgtggctta attttggcat actcaatacc tgagctatcc gtaatctttt
    24121 catcatatga caaaatttca aggaaaaaag gaatgtcttc tgcaacacat tcagaaccaa
    24181 tccgttcaat ataagcttct ttttcttcgt ttacttcatc cgaactatca acatcataat
    24241 aaagtaagaa cttaactgcg tctgccccct cttctttaat gcgtttcgca gaccaaatgt
    24301 ctaaacagtc tgggagtcgc ttggtactac tggtatcata acctgttttt tcgtacgcaa
    24361 gtaaaagacc ggcattgtca tctaaaactt tcgtagctgg caacccatat tctggatcta
    24421 acaacattga tgaagcgtaa ggggttaatt cttcagctac caaaacttta agtgcttcta
    24481 tttgagaaac agtgggctcc tcagattgat attttgccat taatcgcttc aatgctccac
    24541 gttggtcaaa agccaaggcc gaaataatac catttttatc actcaattgt tccatatgtt
    24601 tttgtttttg ttccgttaat cccatcctat acctctttca cttggatttg actgattaat
    24661 tcttgatagt aggtcagatt gatatgccct gttaatgttt cttgtgcatt aagcataccc
    24721 aagacattag ctctcttaag taaatttata tcatctagtt gatgttctaa agcagacgcg
    24781 attcctgcaa ccgttgcatc ccctgaacca actggattga taacttctat ttttggaatc
    24841 gtcacttgat agtagtgatt cccatgttta gcaaaagctc ccctagcacc taaggaaaca
    24901 ataacccaat caattcctct aaaaatatct tgctgtaagg tagattttaa ttcgtctaaa
    24961 ctatagctaa ttggctttcc aattaattgt gttaattctt caaggttagg tttaatgacg
    25021 gtcggcttag cactagattt taaaaccgaa cgtaatgctt ctcctgaaca gtctaaaaca
    25081 actttcttat taaagtgatt cgctagggag attaacctag cataataatc tgaggggagt
    25141 ccctttggta aacttcctga tactgttatg atagttgcag catcaaagat aagttttaga
    25201 tgattcaaaa aggcgcctgc ttcatcttta gtaatatgtg gacctttttc tagaatttca
    25261 gtctgctgac cctcatgtaa aactgcaata caatgacgtg tttctccacc aatacttaaa
    25321 aattggtgtc tgatttccaa tgcatctaag ccacttcgga tgaaatctcc aaaatgcccc
    25381 cctaatagac cagttgccac tacattatcg ccaagttgag aaagaacacg agtcacattt
    25441 aatcccttac caccaggtgt tttggagacg tcggtaaccc gatt
//
LOCUS       DATRUK010000025        25104 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.025, whole genome shotgun sequence.
ACCESSION   DATRUK010000025 DATRUK010000000
VERSION     DATRUK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25104
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.025"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            69..719
                     /locus_tag="VBE34_000988"
     CDS             69..719
                     /locus_tag="VBE34_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001900118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO7263716.1"
                     /translation="MCKYKAIIFDMDGVLFDTELFYYKRRERFLKQHGITINHLPMNF
                     FIGGNMKQVWKSVLGDQYDTWDIDKLQQDYSRYKEDNPLPYKDLIFQDCKRVIEKLHH
                     KGYLLGLASSSTRHDIMLALESFNLDTYFKVILSGEEFSESKPNPAIYNRAAELLDIP
                     KQQILIVEDSEKGIAAGIAAGIDVWAIEDKYFGMNQSQANVLVSDLSQFFVKLDNY"
     gene            857..1648
                     /locus_tag="VBE34_000989"
     CDS             857..1648
                     /locus_tag="VBE34_000989"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEO7263717.1"
                     /translation="MKFENIIYGIDGSVATIMLNRPDISNGFNIPMCQEIIDAIRLMS
                     ENKDVMFLVIEAQGPIFSIGGDLKVMKDAVESDDISSLTKIAELVNQISYDLLQLEKP
                     VVMCVDGAVAGAAANIALAADFVIASKKSKFIQAFVGVGLAPDAGGLLLLSKSIGITR
                     AVQLALTGESLSAEKAEALGIVYKLCESDKIGKIKDQLLKRLSRHSINSYQAIKSLAW
                     EAAFKDWEQYKKLELQLQESLAFKQDFKEGVRAHADRRRPNFLGK"
     gene            1744..2178
                     /locus_tag="VBE34_000990"
     CDS             1744..2178
                     /locus_tag="VBE34_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7263718.1"
                     /translation="MEYDQINSYLVDIFNRIMIIEEMSLKTSQFSDVSLKEMHTIEII
                     GKHSEVTPSDVARELMLTLGTVTTSLNKLEKKGYIERKRSSIDRRVVHLSLTKRGRLL
                     DRLHSKFHKSMVSHIIEDLGEEDIKMLTSALGNLHKFLEDLV"
     gene            2178..3149
                     /locus_tag="VBE34_000991"
     CDS             2178..3149
                     /locus_tag="VBE34_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEO7263719.1"
                     /translation="MVFAKISQLAHYAPSQIIKNEDLSLIMDTSDDWISSRTGIKQRH
                     ISKNETTADLANKVAEQLIEKSGYSASQIDFIIVATMTPDSMMPSTAARVQAHIGASN
                     AFAFDLSAACSGFVFALSTAEKLISSGSYQKGLVIGAETVSKVLDWTDRGTAVLFGDG
                     AGGVLLEASKEKHFLAESLNTDGSRQGLQSSQVGLNSPFSDEVLDDKFLKMDGRAIFD
                     FAIKEVSKSINHLIETSHLEKEDIDYLFLHQANRRILDKMSRKIDIARDKFPENMMDY
                     GNTSAASIPILLSESYENGLLKLDGNQTILLSGFGGGLTWGSLIVKI"
     gene            3207..3431
                     /locus_tag="VBE34_000992"
     CDS             3207..3431
                     /locus_tag="VBE34_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO7263720.1"
                     /translation="MAVFEKVQEIIVEELGKDAEEVTLNTTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKVK"
     gene            3586..4545
                     /gene="fabK"
                     /locus_tag="VBE34_000993"
     CDS             3586..4545
                     /gene="fabK"
                     /locus_tag="VBE34_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEO7263721.1"
                     /translation="MKTRITELLNIKYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSMTDKPFGVNIMLLSPFVDDIVDLVIEEGVKVVTTGAGNPGKY
                     MERFHEAGITVIPVVPSVALAKRMEKLGADAIITEGMEAGGHIGKLTTMTLVRQVVDA
                     VTIPVIAAGGIADGRGAAAGFMLGADAVQVGTRFVVAKESNAHPNYKAKILKAKDIDT
                     AVSAQVVGHPVRALKNKLVTTYSQAEKDYLAGRISINEIEELGAGALRNAVVDGDVIN
                     GSVMAGQIAGLIKSEETCQEILEDIYSGARQVILSEASRWSDL"
     gene            4565..5491
                     /gene="fabD"
                     /locus_tag="VBE34_000994"
     CDS             4565..5491
                     /gene="fabD"
                     /locus_tag="VBE34_000994"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEO7263722.1"
                     /translation="MNKVAFLFAGQGAQKLGMARDLYETFPIVKETFDKASHVLGYDL
                     RELIDKDLDKLNQTKYTQPAILTTSTAIYCLILKEIGLRPDMVAGLSLGEYSALVASG
                     AIRFEDAVVLVSRRGQLMEAAAPAGSGKMVAVLNADRQIIEDACKKASQFGIVSPANY
                     NTPKQIVIGGESIAVNAAVEELKQQGVKRLIPLNVSGPFHTALLKPASQKLSDVLDKV
                     HFSVSEIPVIGNTEAQIMKKDDIKSLLARQVMEPVRFDESIETMKKMGMTQVVEIGPG
                     KVLSGFLKKIDSSLSVYSVEDKIGFNNLKELN"
     gene            5500..6234
                     /gene="fabG"
                     /locus_tag="VBE34_000995"
     CDS             5500..6234
                     /gene="fabG"
                     /locus_tag="VBE34_000995"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEO7263723.1"
                     /translation="MQLKDKNIFITGSSRGIGLAIAHQFAQLGANIVLNGRSEISEDL
                     IAEFADYGVKVIAISGDVSSFEDANRMIKEAIASLGSVDVLVNNAGITNDKLMLKMTV
                     EDFESVLKINLTGAFNMTQSVLKPMTKARQGAIINISSVVGLTGNVGQANYAASKAGL
                     IGFTKSVAREVAARGIRVNAIAPGFIESDMTDVIPEKMQEAILAQIPMKRIGKGKEVA
                     QVASFLAEQEYLTGQVIAIDGGMTMQ"
     gene            6250..7482
                     /gene="fabF"
                     /locus_tag="VBE34_000996"
     CDS             6250..7482
                     /gene="fabF"
                     /locus_tag="VBE34_000996"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEO7263724.1"
                     /translation="MTLQRVVVTGYGVTSPIGNTPEEFWNSLKEGNVGIGPITKFDSS
                     DFMVKNAAEIHDFPFDKYFVKKDLNRFDMYSLYALYASSEAIQHANLNLDEIDADRFG
                     VIVASGIGGIQEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMRLGAHGVCKSI
                     NTACASSNDAIGDAFRNIKFGIQDIMVVGGAEAAITKFAIAGFQSLTALSTTEDPSRA
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     LGATKAIQLALAEANIKPEEVNYVNAHGTSTPANEKGESQAVVAALGTDVPVSSTKSF
                     TGHLLGAAGAVEAIATIEAIRHSYVPMTAGTTELSEDITANVIFGQGQDADIRYAISN
                     TFGFGGHNAVLAFKRWED"
     gene            7448..7984
                     /locus_tag="VBE34_000997"
     CDS             7448..7984
                     /locus_tag="VBE34_000997"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEO7263725.1"
                     /translation="MQFWPSNAGRIKLDIQEIKDLMTQFDESSLREFSFKTSDGELSF
                     SKNEGKAPLVPTMSPMSHQPEATPTIATPVSNEAGEQTKQATEVVSEIPESTVTVAEG
                     DVVESPLVGVAYLASGPDKPNFVSVGDSVKKGQTLMIIEAMKVMNEVPAPHDGVVTEI
                     LVANEEVIEFGKGLVRIK"
     gene            7981..8403
                     /gene="fabZ"
                     /locus_tag="VBE34_000998"
     CDS             7981..8403
                     /gene="fabZ"
                     /locus_tag="VBE34_000998"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO7263726.1"
                     /translation="MIDIKEIREALPHRYPMLLVDRVLEVSEDEIVAIKNVSINEPFF
                     NGHFPEYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTAKFVKRRGTIAVVEAIAEVDGKLAASGTLTFAIGN"
     gene            8441..9811
                     /gene="accC"
                     /locus_tag="VBE34_000999"
     CDS             8441..9811
                     /gene="accC"
                     /locus_tag="VBE34_000999"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEO7263727.1"
                     /translation="MFKKILIANRGEIAVRIIRAAREMGISTVAIYSEADKESLHTIL
                     ADEAICVGPAKSAESYLNVNAILSAAIVTGAEAVHPGFGFLSENSKFATMCEEMNLKF
                     IGPSGEVMDKMGDKINARAEMIKADVPVIPGSDGQVTSVEEAVSIAEEIGYPLMLKAS
                     AGGGGKGIRKVKSADELKPAFESASQEALAAFGNGAMYIEKVIYPARHIEVQILGDSF
                     GKIVHLGERDCSLQRNNQKVLEESPSVAIGNTLRQQIGEAAVRAAEAVSYENAGTIEF
                     LLDENSGQFYFMEMNTRVQVEHPVTEFVTGVDIVKEQIRIAAGIPLSVSQNDIKLTGH
                     AIECRINAENPQFNFAPCPGTINGLHLPAGGMGLRVDSAVYTGYTIPPYYDSMIAKVI
                     VHGENRFDALMKMQRALYELEIDGIITNTEFQMDLISDKKVLAGDYDTSFLMEDFLPR
                     YYGNNQ"
     gene            9820..10695
                     /locus_tag="VBE34_001000"
     CDS             9820..10695
                     /locus_tag="VBE34_001000"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEO7263728.1"
                     /translation="MALFSKKDKYIRISPNKALGSSDKRSLPEVPDELFAKCPSCKHM
                     IYQKDLGLAKICPACSYNFRISAQERLLLTVDEDSFEELFTGIETKDPLNFPNYREKL
                     AATRQKTNLDEAVVTGLAKIKGQTTALAIMDSHFIMASMGTVVGEKLTRLFELATEKK
                     LPIVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNQGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQALVGFAGRRVIETTVREDLPEGFQKAEFLLEHGFIDAIINRTELRDC
                     IAQLIAFHGNEVGHE"
     gene            10688..11461
                     /locus_tag="VBE34_001001"
     CDS             10688..11461
                     /locus_tag="VBE34_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEO7263729.1"
                     /translation="MNNDVTRILKDARDQGRLTALDYAELIFDNFMELHGDRQFADDK
                     SIIGGLGYLAGRPVTIVGIQKGKNLQDNLDRHFGQPHPEGYRKALRLMKQAEKFGRPV
                     ITFINTAGAYPGVGAEERGQGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALAVAD
                     KVWMLEHTVYSILSPEGFASILWKDGTRTTEAAQLMKMTAGELYHMEVVDKVIPEHGY
                     FSSEIVDMIKTSLISELEMLSQLSLEDLLEQRYQRFRKY"
     gene            11929..12561
                     /locus_tag="VBE34_001002"
     CDS             11929..12561
                     /locus_tag="VBE34_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEO7263730.1"
                     /translation="MYQELNDYLEFQSMKYIMPEKAGPLKDDMQTFKAKGQLARNSFT
                     ELSRALEQRMDGFKMQRVSNWANQAQVGRPHFWVYYRKDTDQLDDVAVALRVYGVKDS
                     FGVSLEVSFVERQKSDKTLEKQARVLSIPIASPLYFMVQRQGETHREEGNEENRQRLM
                     QEIKSGKVRKVLVKYDVLLTENQSLENILQRLLEGFEKVLPYYQVCQTIS"
     gene            complement(12607..13884)
                     /gene="serS"
                     /locus_tag="VBE34_001003"
     CDS             complement(12607..13884)
                     /gene="serS"
                     /locus_tag="VBE34_001003"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEO7263731.1"
                     /translation="MLDLKRIRTDFDAVAKKLATRGVDQETLTTLKELDIKRRELLIK
                     SEEAKAQRNVASAAIAQAKRNKENADEQITAMQTLSADIKAIDAELADVDANLQSMVT
                     VLPNTPADDVPLGADEDENVEVRRWGTPREFDFEAKAHWDLGESLGILDWERGAKVTG
                     SRFLFYKGLGARLERAIYSFMLDEHAKEGYTEVIPPYMVNHDSMFGTGQYPKFKEDTF
                     ELADSPFVLIPTAEVPLTNYYRDEIIDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLNLPYRVITLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEVDGKVRLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGNIDIIKPN"
     gene            14183..15244
                     /locus_tag="VBE34_001004"
     CDS             14183..15244
                     /locus_tag="VBE34_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEO7263732.1"
                     /translation="MEKYTSIKQSGRHYGLDLLRIISMFMIVITHVLGKGGLRSSVEG
                     HADSYFIVTWIIQVLVYGAVNCYALISGYVGINSRYRYSKLLSIWTQVFFYTFTITAL
                     FAITGHEVTLLNWRDAFFPIVSGQYWYITAYFGLLVFMPVINNGLNALTDKQLKQLVL
                     LMFIIFSILPAVLNNRVPEFSLSKGFEMTWLLILYIIGAYLKRIDLNIFKTSYLLIVY
                     LLSLVATYVMKFSIGDIWYWYVSPTLTLGAVSLFILFARASIKPSGFLKKIIVVLAPS
                     TLGVYLCHLHPLIVKYFVRDFAETFVYESIYLYPFLILGAGILIYLLAFIVERIRLRI
                     FKAIHFSNFLRWFDDKFPY"
     gene            complement(15282..15644)
                     /locus_tag="VBE34_001005"
     CDS             complement(15282..15644)
                     /locus_tag="VBE34_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEO7263733.1"
                     /translation="MAQSLNKTVEFQTTGVSYLGMGNKVGKFLVGDQALEFYNDKNVN
                     DYIQIPWTSINQIGANVSRKKISRHFEVFTDQGKFLFASKDSGTILKHARRHIGDDKV
                     VKLPTLIQTILKIFKFKK"
     gene            complement(15763..16674)
                     /locus_tag="VBE34_001006"
     CDS             complement(15763..16674)
                     /locus_tag="VBE34_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO7263734.1"
                     /translation="MTEQIKLSKSDRQKVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTTKEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEEKASGTPVEDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASAGNILGPIIFFVGWNLIRMSFLWYTQE
                     LGYKSGKEITKDMSGGILQDITKGASILGMFILAVLVKRWVAINFTVDLPKKTLSEGA
                     YINFPKDHVSGQQLHDILGQVQSGLSLDKMQPQTLQGQLDSLIPGLAGLLLTFFCMWL
                     LKKKVSPITIIIGLFIVGILARLAGVM"
     gene            complement(16689..17501)
                     /locus_tag="VBE34_001007"
     CDS             complement(16689..17501)
                     /locus_tag="VBE34_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEO7263735.1"
                     /translation="MADISIISMILVVIVAFFAGLEGILDQFQIHQPLVACTLIGLVT
                     GHLEAGIILGGSLQMIALAWANIGAAVAPDAALASVAAAIILVKSGDFTQKGINFAFS
                     TAVPLAIAGLFLTMIVRTISTALVHAGDKAASEGNFAAIERFHFIALLLQGLRIAFPA
                     ALLLAIPSSSVQSILEAMPDWLNGGMQVGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAALNQLTLIAMGTIGVAIALIYISLSKMGGSKGTSNAGSNDPIGDILEDY"
     gene            complement(17534..18544)
                     /gene="manX"
                     /locus_tag="VBE34_001008"
     CDS             complement(17534..18544)
                     /gene="manX"
                     /locus_tag="VBE34_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEO7263736.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIAQFDADDEVLVLADLWSGSPFNQASRVMGENPERKMAIITGLNLPMLIQ
                     AYTERMMDANAGVEQVAANIIKESKEGIKALPEELNPVVEATPVAGVPADVPAEVKQS
                     GSIPEGTVIGDGKLKINLARIDTRLLHGQVATAWTPASKANRIIVASDEVSKDELRKQ
                     LIKQAAPGGVKANVVPISKLIEVAKDPRFGNTRALILFETVQDALRAVEGGVEIPELN
                     VGSMAHSTGKTMVNNVLSMDKDDVAAFEKLRDLGVSFDVRKVPNDAKKNLFDLINKAN
                     VK"
     gene            complement(18846..19658)
                     /locus_tag="VBE34_001009"
     CDS             complement(18846..19658)
                     /locus_tag="VBE34_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7263737.1"
                     /translation="MTKKIIAVDLDGTLLHNNNTISDYTADTLRKVQAQGHKVIITTG
                     RPYRMALAHYLRLDLKTPMINFNGALTHIPEKKWAFEHSATIDKKLLLETLNLSDAIQ
                     ADFIASEYRKNFYITMDNRDKINPQLFGVNEITDKMALDVTKITRNPNALLMQTRHKD
                     KYELAKELRQHFNHELEVDSWGGPLNILEFSPKGVNKAYALKHLLKSLNLSQENLIAF
                     GDEHNDTEMLAFAHTGYAMKNASPTLLPYADQQIQWTNEEDGVAKTLEKLLL"
     gene            19747..20331
                     /locus_tag="VBE34_001010"
     CDS             19747..20331
                     /locus_tag="VBE34_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263738.1"
                     /translation="MKKLIWSHLFAIVSILGIWYYHVKIDHLMVILLASVLSLEMGFI
                     AYYSRNIFIRLLASLGLLFFYPQSLGIFLSLVSLEWSTKDIWQSNHFMQYMAYLLAIL
                     FIISSALLSLKLLVNAYDISKRYQLATVIPVMLFSSFAFCLEKSGSLGLEGIILNPSA
                     FFNGIQTNMANLDFKALSCLFLVQLLLWGILMED"
     gene            20421..21032
                     /locus_tag="VBE34_001011"
     CDS             20421..21032
                     /locus_tag="VBE34_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEO7263739.1"
                     /translation="MKVKLFAIHLFFIVISFGIKYYHLQGPNLIWNMTLALIALDFAY
                     LTSLFKKKILIGLFALAWFFFYPNTFYMLTDIIHMHFVGDVLYNKTNLILYILYVSSI
                     LFGFLSGIESFSVIMRKFRISNIFLRWGIIGIVSFVSSFGIHIGRYARLNSWDILTKP
                     QVVINELLAVPSRDSFHFILGFTFLQVLCLIFMDYSAGKKLEK"
     gene            21135..22556
                     /locus_tag="VBE34_001012"
     CDS             21135..22556
                     /locus_tag="VBE34_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEO7263740.1"
                     /translation="MEKFFKLKEHGTTIRTEITAGLTTFFAMSYILFVNPAILSQTGM
                     PAQGVFLATIIGAVVATSVMAFYANLPYAQAPGMGLNAFFTYTVVFALGYTWQEALAM
                     VFICGLISLIITLTKVRKMIIESIPTTLKSAITAGIGTFLAYVGIKNAGFLKFSIDPG
                     TYDVVGKGAAKGLATITANSSATPGLVSFDNPAILLSLIGLSITIFFIVKGIRGGIIL
                     SILTTTLLGILMGVVKLDAINWEATNLSASFRDLKQVFGVALGEKGLISLFSNPSRLP
                     SVLMAILAFSLTDIFDTIGTLIGTGEKVGILATTGDNHESKSLDKALYSDLIGTTFGA
                     ICGTSNVTTYVESAAGIGAGGRTGLTALVVAGLFAISSFFSPLVSIVPSQATAPILVI
                     VGIMMLSNLKDIKWDDMSEAIPAFFTSLFMGFTYSITYGIAAGFLTYTLAKVIKGQAK
                     DIHVVLWILDILFILNFISLAIL"
     gene            22639..23148
                     /gene="tsaE"
                     /locus_tag="VBE34_001013"
     CDS             22639..23148
                     /gene="tsaE"
                     /locus_tag="VBE34_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEO7263741.1"
                     /translation="MLWGCQNQQTVGRQRYFWYNISMFYTQNEEELIALGQKLGTVLK
                     SGDIVLLTGNLGAGKTTLTKGIAKGLDIKQMIKSPTYTIVREYEGRVPLYHLDVYRIG
                     DDPDSIDLDDFLFGQGVTVIEWGELLSDNLINNYLEIVITRSNQGRQVQLEAYGHRAR
                     EIIEAIQDV"
     gene            23102..23662
                     /locus_tag="VBE34_001014"
     CDS             23102..23662
                     /locus_tag="VBE34_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001890427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7263742.1"
                     /translation="MGIAQEKLLRRFKMSKQFAVDFIEASREEASALLEFLNTVTEET
                     DFILHTVSNQLSLPEMETFIENTLITKNCICLIAKLKNKVIGLITIISQSDIEIEHIG
                     DLFIAVQKDYWGYGIGHILMEEAIEWASDNDITRRLELSVQGRNERAIHLYQKFSFEI
                     DGLQTRGIKRENGEFLDIYRMSKLID"
     gene            23671..24978
                     /locus_tag="VBE34_001015"
     CDS             23671..24978
                     /locus_tag="VBE34_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO7263743.1"
                     /translation="MKIWKKITLMFSAIILTTVIALGVYVASAYNFSTNELSKTFKDF
                     KLAKSKSHAIEETKPFSILLMGVDTGSEHRKSKWSGNSDSMILVTINPKTNKTTMTSL
                     ERDVLIKLSGPKNNGQTGVEAKLNAAYASGGAEMALMTVQDLLDINVDYFMQINMQGL
                     VDLVNAVGGITVTNKFDFPISIAANEPEYKAVVEPGTHKINGEQALVYSRMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALNSISSYKKILSAVSNNMQTNIEISSKTIPNLLAYKD
                     SLEHIKSYQLKGEDATLSDGGSYQILTKKHLLAVQNRIKKELDKKRSKTLKTSAILYE
                     DYYGTTASNDSSTYSSTQENNYNTTPYSEAPPSYSGNTTYSSETNQTTHQSYYNSSTP
                     ASNYSSNTNTGQADSSGSVNNYNGAATPNPNTGTQPVPSQTNP"
BASE COUNT         7987 a         3909 c         5103 g         8105 t
ORIGIN      
        1 ataaattata acaaaagtca gaaaatttgt cattacctct aaatttaaaa gaatgatata
       61 atagttacat gtgtaaatat aaagcaataa tatttgatat ggatggtgtt ctatttgaca
      121 ctgaattatt ttattataaa aggcgagagc gttttttaaa acaacatgga attacaatta
      181 accatttacc aatgaatttt tttattggag gaaatatgaa gcaggtttgg aaatctgttt
      241 taggtgatca atatgatacc tgggatattg ataagctcca acaagattat agtcgctata
      301 aagaggataa tcccttgcca tacaaagatt taatttttca ggattgtaaa agagtcattg
      361 aaaagcttca tcacaaaggc tatcttttgg gtctagcttc aagttctact agacatgata
      421 tcatgttagc attagaaagt tttaatctgg atacatattt taaggtaatt ctgtctggtg
      481 aggaattttc agaaagtaaa cctaatccag ctatttataa tagagcagca gaacttttag
      541 atattcctaa acagcaaatt cttattgtag aagacagtga gaaggggatt gcagctggca
      601 ttgcagctgg tattgatgtg tgggctattg aagacaaata ctttggaatg aatcagtcgc
      661 aggctaatgt attagtgagc gatctgagtc aattttttgt gaaattagat aactattaaa
      721 aacgggaggt ttaattccgt tttttttatt ttttatcttg ccatgaaagc taggtttatg
      781 ataaaataca aagcatgata atttgataat caaattattt tttatttttc tttcactctt
      841 aaagagagga gacgttttga agttcgaaaa tataatatat ggtattgatg gcagcgtggc
      901 cactattatg cttaatcgac cagatatttc aaatggtttt aacatcccaa tgtgtcaaga
      961 aattattgat gcaataaggc ttatgtctga aaataaagat gtcatgtttt tagtgattga
     1021 agcacaagga ccaatctttt caatcggagg agatttaaaa gtgatgaaag atgctgttga
     1081 gagcgatgat attagctcat taactaaaat tgctgagtta gttaatcaga tatcgtatga
     1141 tctactacag ctagagaaac ctgttgttat gtgtgtagat ggggctgtag ctggtgccgc
     1201 agctaatatt gcattagcag ctgattttgt tatcgcaagc aagaagagta aatttatcca
     1261 ggcttttgtg ggtgtcggtt tagctccaga tgcaggaggt ttattgttac ttagtaaatc
     1321 aatcggtata actcgtgcag tgcaactagc tttaactgga gaaagcttat ctgctgaaaa
     1381 agctgaagct ttgggaattg tttataaact ttgtgaatca gataaaatag gtaaaattaa
     1441 ggatcaacta ttaaagcgtt taagtagaca ttctataaac tcatatcaag ccataaaatc
     1501 attagcttgg gaagctgctt ttaaagattg ggaacaatat aaaaagcttg aattacagtt
     1561 acaagaatca ttagccttta agcaagattt taaagagggt gttcgggcac acgctgatag
     1621 aaggcgtcct aattttttag gcaaatagtt tgaaaatcaa attatttaag attaaagtct
     1681 tgcaaataag tttgaacttt gatataattt gactatcaaa gtttaatgtg aggtggtgat
     1741 atcttggaat acgatcagat taatagttat ttagttgaca tttttaatag aatcatgatt
     1801 attgaggaaa tgagtttaaa gactagccaa tttagcgatg tatctttaaa agaaatgcat
     1861 actattgaaa ttattgggaa acattctgag gtaactccta gcgatgtcgc tcgagaatta
     1921 atgttaactt tgggaactgt aacaaccagc cttaataagt tagaaaaaaa aggctacatt
     1981 gagcgtaaac ggtcaagtat tgatcgtcga gttgttcact tgagtcttac taagagaggt
     2041 aggttattag atcgattgca tagtaaattt cataaatcta tggtatctca tattattgaa
     2101 gacctaggcg aagaagacat aaaaatgttg acgagcgctt tagggaatct tcataaattt
     2161 ttggaggact tagtctagtg gtttttgcta aaattagtca acttgcacat tatgcacctt
     2221 ctcaaatcat taaaaatgag gatttatcat taatcatgga taccagtgat gactggattt
     2281 cgtcacgtac tggtataaaa caacgtcata ttagtaaaaa tgaaacaaca gctgatttgg
     2341 caaataaggt cgcagaacaa cttattgaaa aatccggtta ttccgctagt caaattgact
     2401 ttattattgt tgcaacgatg acaccagact ctatgatgcc ttctactgcc gcaagagtgc
     2461 aagctcatat tggagcgagt aatgcttttg cctttgactt gagtgcagca tgtagtggtt
     2521 ttgtttttgc cttgtctacc gccgaaaaat tgatatcatc aggtagctat caaaaaggtt
     2581 tagtaatagg agcagaaact gtttctaaag tattagactg gactgataga gggacagcag
     2641 ttttatttgg agatggagct ggaggtgtct tgctagaagc ttctaaggaa aaacattttc
     2701 ttgcagagtc tctcaataca gatggtagtc gtcagggact tcaatctagt caagtcgggt
     2761 taaattcacc tttctcagat gaagtacttg atgataaatt tttgaaaatg gatggtagag
     2821 caatctttga ttttgctatc aaagaagttt caaaaagtat caatcatctt atagaaacat
     2881 cccatttgga aaaagaagat attgattatc ttttcttaca ccaagcgaat cgaaggattt
     2941 tagataagat gtctcgaaaa attgatatag cacgtgataa atttccagag aatatgatgg
     3001 attatggtaa tactagtgca gctagtatcc ctattttact ttcagaatct tatgagaacg
     3061 gtttgttgaa actagatggt aaccaaacaa tcctcttatc gggtttcggt ggaggtttga
     3121 catggggaag tctaattgtt aaaatttagt tatatatagt gcatagcaca tttataaaag
     3181 attatttaaa atttaaggag ctaaaaatgg cagtatttga aaaagtacaa gaaattattg
     3241 ttgaagaact tggtaaagat gcagaagaag taacgttgaa cactactttt gacgatcttg
     3301 atgctgattc gcttgatgtt ttccaagtga tttcagaaat tgaagatgca tttgatatcc
     3361 aaattgagac agaagaagga ctaaatactg ttggtgattt agttgcttat gttgaagaaa
     3421 aagtaaaata attaagtcgt aagaaaaaaa gaagcaagtg ataataaaag atattcttac
     3481 caaatcactt attagagtga gtattcaatc aatcgttgat cggtactcac tcttcttctt
     3541 atcaatatag tttgattgtc aaattattaa ttttaggaaa gaaaaatgaa aacacgtata
     3601 acagaattac taaatataaa atatccaatt tttcaagggg gtatggcgtg ggtcgctgat
     3661 ggagatcttg caggagctgt ttctaaggca ggtggtctag gtattattgg tggaggaaat
     3721 gcccctaaag aggttgtcaa agcaaatatt gataaaataa aatctatgac tgataaaccc
     3781 tttggtgtga atattatgct tttatcacct tttgtagatg atattgttga tttggttatt
     3841 gaagaaggtg taaaagttgt aacgactggt gcaggtaatc ctggtaagta catggaacgc
     3901 tttcatgaag caggtataac agtaatccct gttgttccta gtgttgcatt agcaaaacgt
     3961 atggagaaat taggggctga tgctatcatt acagaaggaa tggaagcggg tggtcacatc
     4021 gggaagctga ccacgatgac attagtgcga caggttgttg atgctgttac aattccagtc
     4081 attgccgcag gtggtatagc agatgggcgt ggggctgctg cgggtttcat gttaggagct
     4141 gatgctgttc aggtaggaac tcgttttgtt gttgctaaag aatctaatgc tcaccctaat
     4201 tacaaagcaa aaattttaaa agcaaaagat atcgacactg ctgtttctgc tcaggtagta
     4261 ggtcatccag ttcgtgcctt aaaaaataaa ctagtaacaa cctatagcca agcagaaaaa
     4321 gattatttag ctggccgtat ttcaatcaat gagattgaag aattaggagc gggagctctt
     4381 cgcaatgcag ttgttgatgg ggatgttatt aatggttcag ttatggcagg acaaattgca
     4441 ggattaatta aatcagaaga gacttgtcaa gagattcttg aagatattta ttcaggagcg
     4501 cgtcaagtta ttctttctga ggcaagccgc tggtcagatt tataaataca aataaggaga
     4561 cactatgaat aaagttgcat ttctatttgc tggtcaaggt gctcaaaagt taggaatggc
     4621 tagagattta tatgaaactt tcccgattgt taaagaaact tttgataagg caagtcatgt
     4681 tttaggatat gatttgagag aattgattga taaagactta gacaaattaa atcagacgaa
     4741 atatacgcaa ccagctattt taacgacttc gactgctatt tattgtttaa ttcttaaaga
     4801 aattggactt agaccagata tggttgcagg actttctttg ggagaatata gtgctttagt
     4861 tgcttcaggt gctataagat ttgaagatgc tgtagtactt gtttctagac gaggccaact
     4921 aatggaagca gcagcacctg ctggtagtgg caaaatggta gcggttttaa atgctgatcg
     4981 tcaaataatt gaagatgctt gtaagaaggc ttctcagttt ggtattgtta gtcctgcaaa
     5041 ttataatact ccaaagcaaa ttgttatagg gggagagtca atcgctgtaa atgcagctgt
     5101 agaagaacta aaacaacaag gagtaaaacg tttaattcct ctgaatgttt caggtccttt
     5161 tcacactgct ttattgaaac ctgcaagtca gaaattgagc gatgttttag ataaagttca
     5221 tttttcagtt tctgaaatcc ctgtaattgg aaatacagag gcgcagataa tgaaaaaaga
     5281 cgatatcaaa tctttattgg cacgccaagt tatggaacct gtacgctttg atgagtctat
     5341 tgaaaccatg aaaaaaatgg gaatgacaca ggttgttgaa attggtccag ggaaggtact
     5401 ctcaggtttc ttgaaaaaaa tagatagctc attgtcagta tattctgttg aagataaaat
     5461 tggatttaat aatcttaagg aattaaacta ggggaaaata tgcaattaaa agataagaac
     5521 atttttatta cgggttcaag tagaggaatc ggccttgcaa ttgcccatca atttgcacaa
     5581 ttaggtgcta atattgtttt aaatggacgt tctgaaatat cagaagactt aatagctgaa
     5641 tttgctgatt atggtgtaaa agttattgct atttcaggag atgtttcttc ttttgaagat
     5701 gctaatcgca tgattaaaga agctattgca agtctaggta gcgtggatgt gcttgttaac
     5761 aacgctggta ttactaatga taaattgatg cttaaaatga ctgttgaaga ttttgagagc
     5821 gttcttaaaa ttaatttaac aggtgcattt aatatgacac aatcagtctt gaaaccgatg
     5881 acaaaagcgc gtcaaggtgc tattattaat atctctagcg tcgttggatt aactggaaat
     5941 gtagggcaag ctaattacgc agcctctaaa gcaggtttaa ttggctttac aaaatcagtt
     6001 gccagagaag tagcagcacg aggcattcgt gtcaatgcca ttgctccagg ctttatcgaa
     6061 tctgatatga cagatgttat cccagaaaaa atgcaagaag ctattttagc tcaaattcca
     6121 atgaaacgta ttggaaaagg taaagaagta gcgcaggtag cgtctttctt agcagaacaa
     6181 gaatacctta caggacaagt tattgccatt gatggcggta tgacgatgca ataataaaga
     6241 ggaaataata tgacattaca gcgagtagta gtaacaggtt acggtgtaac ctcacctatt
     6301 ggaaacacac cagaagaatt ttggaatagc cttaaagaag gaaatgttgg tattggacct
     6361 atcactaaat ttgattcaag tgatttcatg gtcaaaaatg ctgctgaaat tcatgatttt
     6421 ccatttgata aatattttgt caaaaaagat ctgaacagat ttgatatgta ttccctttat
     6481 gccttatatg cttcaagtga agctattcaa catgctaatt taaatttaga tgaaattgat
     6541 gcagatcgat ttggtgtcat cgttgcatca ggtattggag ggattcaaga gattgaagaa
     6601 caagtgattc gtcttcatga aaaagggcca aaacgcgtaa aaccaatgac gcttccaaaa
     6661 gctctaccaa atatggcagc tggaaatgtt gcgatgaggt taggcgctca tggtgtatgt
     6721 aaatcaatta atacagcttg tgcttcttct aatgatgcta ttggagatgc tttccgtaat
     6781 ataaaatttg gtattcaaga tattatggta gtcggtggtg cagaagctgc tattacaaaa
     6841 tttgccattg ctggattcca aagtcttaca gcattatcaa caactgaaga tccatcaaga
     6901 gcatctattc catttgataa agatcgcaat ggatttatca tgggagaagg ttctggaatg
     6961 ttggtcttag aaagtttaga acacgctgaa aaacgaggag ctactattct tgcagaagtt
     7021 gttggttatg gtaacacttg tgacgcatat catatgactt caccacatcc agaaggttta
     7081 ggagccacta aagctattca acttgcactg gctgaagcta atattaaacc tgaagaagtt
     7141 aactatgtaa atgctcatgg aacttcaaca cctgctaacg aaaaaggaga gagtcaagct
     7201 gttgttgctg cacttgggac agacgttcca gtttcttcga ctaaatcttt tactggtcat
     7261 cttttgggtg ccgcaggagc tgttgaggca attgcaacta ttgaagcgat acgtcatagt
     7321 tatgttccaa tgactgctgg aacaacagaa ttatcagaag atattacagc gaatgttatt
     7381 tttggtcaag ggcaagacgc tgatattcgt tatgccattt caaatacatt tggttttgga
     7441 ggacacaatg cagttctggc cttcaaacgc tgggaggatt aaattggata ttcaagagat
     7501 taaggattta atgactcaat ttgatgagtc aagtctgaga gaattttcat tcaaaacaag
     7561 tgacggtgag ctgtctttta gtaaaaatga gggaaaagct ccacttgtac caactatgtc
     7621 tcctatgagt catcaacctg aagcgactcc aactatagca actcctgtct ctaatgaagc
     7681 aggtgagcaa acgaaacaag ctactgaagt agtttcagaa atcccagagt cgactgtgac
     7741 tgttgcagaa ggtgacgttg tcgaaagtcc tttagttggg gttgcttatt tagcttctgg
     7801 accagataaa cctaactttg tttcagtagg tgatagcgtg aagaagggac aaactttgat
     7861 gattattgag gcaatgaagg tgatgaatga agtgcctgcc cctcatgatg gtgtagttac
     7921 tgaaattctt gtagccaatg aagaagttat tgaatttggt aaaggattgg tacgaatcaa
     7981 atgattgata taaaagaaat tcgtgaggct ttacctcacc gctatccaat gttattagtg
     8041 gatcgtgttt tagaggtatc agaagatgag atagtcgcta tcaaaaatgt tagtatcaat
     8101 gagccttttt tcaatggtca ttttccagaa tatcctgtaa tgccaggcgt tttaattatg
     8161 gaagctttgg ctcaaactgc aggtgtttta gaattatcta aagaagaaaa caaaggaaag
     8221 cttgtttttt atgcaggtat ggataaagtg aaatttaaaa agcaagttgt tccaggagat
     8281 caattagtta tgactgctaa gtttgtcaaa cgtagaggaa ctatagccgt tgttgaagca
     8341 atagcagaag ttgatgggaa attagctgcc agtggaacgt taacatttgc aataggtaat
     8401 tgaagatagt attgaattta gagggagaga agcatcaaaa atgtttaaga aaatattgat
     8461 tgctaatcgc ggagaaattg cagtacgtat cattcgtgca gcccgtgaaa tgggaatttc
     8521 gacagttgct atctattcgg aggctgacaa ggaatcattg cacacaattt tagcagatga
     8581 agctatttgt gttggaccag ccaaatcagc agaatcttat ttaaacgtta atgctatatt
     8641 atctgctgct attgtgactg gagcggaagc tgttcatcca ggttttgggt ttttgagtga
     8701 aaattctaaa tttgctacta tgtgtgaaga gatgaactta aaattcattg gtccatctgg
     8761 agaagttatg gataaaatgg gtgataaaat caatgctcgt gcagagatga taaaagctga
     8821 tgttccggtt atcccaggat cagatggtca agttacaagt gtagaagaag ctgtatcaat
     8881 tgctgaggaa atcggctatc cattgatgtt aaaagcctca gccggtggcg gtggaaaagg
     8941 aattaggaag gtcaaatcag cagatgaatt gaagcctgct tttgagtctg cttcacagga
     9001 ggctttggct gcgttcggta atggtgctat gtatattgaa aaagttatat acccagctag
     9061 acatattgaa gtacaaatac ttggagatag ctttggtaag atagttcacc taggagaacg
     9121 tgattgttct ctgcagcgta acaatcagaa ggtattggaa gaaagtccgt ctgtggcaat
     9181 aggtaacact ctgcgtcaac aaattggtga agcagctgtt cgtgcagctg aggctgtatc
     9241 ttatgaaaat gcaggaacaa ttgagttttt attagatgag aatagcggac agttctattt
     9301 catggaaatg aatactcgtg ttcaagtaga acaccctgtt acagaatttg taacgggtgt
     9361 tgacatcgtt aaagagcaga ttcgtatcgc tgctggaatt cctttatcag tttctcaaaa
     9421 tgatattaag ttaactggtc atgcgattga atgtcgtatt aacgcagaga atccacaatt
     9481 taattttgcg ccatgtccag gtaccattaa tgggttacat ctacctgctg gcggtatggg
     9541 cttacgtgtc gattcagcgg tttacacagg ctacactatt cctccttatt atgatagtat
     9601 gattgcgaaa gtaattgttc acggagaaaa tcgttttgat gcacttatga aaatgcaacg
     9661 tgccttatat gagcttgaaa ttgatggtat tataacaaat acagaatttc aaatggatct
     9721 catttctgat aaaaaagtct tggctggaga ttatgatact tcattcctga tggaagactt
     9781 tttaccaagg tattatggta acaatcaata ggagggaaga tggctttatt tagtaaaaag
     9841 gataaatata tccgtattag tccaaacaaa gcattagggt cttctgataa acgtagtctt
     9901 cctgaggttc ctgatgaact ctttgctaag tgtccttctt gtaaacatat gatttatcag
     9961 aaagatttag gattagcaaa gatttgtcca gcttgttctt acaattttag aatttcagcg
    10021 caagaacgtc tgttgctaac agttgacgaa gatagttttg aggaattatt tacagggatt
    10081 gaaactaagg atcctttgaa ttttccaaac taccgtgaaa aactggctgc tacacgtcaa
    10141 aagactaact tagatgaagc ggtggtgaca ggtttagcta aaataaaagg acaaactacg
    10201 gccttagcta ttatggattc tcattttata atggcgtcta tgggaacggt tgtaggcgaa
    10261 aagttgacaa gattatttga gctagcaact gaaaagaaat tacctattgt tatttttaca
    10321 gcgtcagggg gcgccagaat gcaagaaggt attatgagtt tgatgcaaat ggcaaaagta
    10381 tcggcagcag ttaaacgcca ttctaaccag ggattgtttt atttaacaat tttaacagac
    10441 ccaacaaccg gcggcgttac tgcgagtttt gctatggaag gtgatattat tctggctgag
    10501 ccgcaagcgt tggttggttt tgctggtcgt cgtgttatcg aaacaacggt gagagaagat
    10561 ttacctgaag gctttcaaaa ggcagagttt ttgttagaac atggttttat tgatgctatt
    10621 ataaatcgaa cagaattacg cgactgcatt gctcaactaa ttgctttcca tggaaatgag
    10681 gttggtcatg aataatgatg tgacaagaat tttaaaagat gcgcgtgatc aagggaggct
    10741 aactgctctt gactacgctg aattgatttt tgataatttt atggaacttc atggtgatcg
    10801 ccagttcgca gatgacaaat caattatagg aggtttgggg tacttggcag ggcgtcctgt
    10861 gacgattgtc ggtattcaaa aaggtaagaa ccttcaagat aatttagacc gtcattttgg
    10921 tcaaccacat cctgaaggtt atcgtaaggc tttacgttta atgaaacaag ctgagaagtt
    10981 tggaagacct gttataacct tcatcaatac tgctggagca tatcctgggg taggtgctga
    11041 ggagcgaggt cagggtgaag caattgctag aaacctctta gaaatgagtg atttaaaagt
    11101 ccctattata gctattatta ttggtgaagg gggttctgga ggagcattag ctttagctgt
    11161 tgccgataag gtttggatgt tggaacatac tgtttattct attttaagtc ctgagggctt
    11221 tgcctctatt ttatggaaag atggtacaag aacaacagaa gcagcacaat taatgaagat
    11281 gactgctgga gaactttacc atatggaagt tgtagacaaa gtaatccctg aacatggtta
    11341 tttttcaagt gagattgtcg atatgattaa gacaagttta atttcagagt tagaaatgct
    11401 ctcacaatta tctcttgagg atttattgga gcaacgttac caacgtttta gaaaatatta
    11461 atagaaaaag gctgagttat ttactcagcc tatatttagt ttttcaaggt attatcatag
    11521 ttatagtttc atattttgct ataattgata aaaaggagat atgtcaatga cactagcatt
    11581 agccaatttt tagattgtag tctaatccaa aattttcaga aagagtcatt tcgaacactt
    11641 tattctaagt accattattt aggctctcct taacttgaga ctctatctgt tattgaagag
    11701 aaattttcta gaaaaaatag ctattgttag attttagaag gctatgaaaa gattggtttt
    11761 taaagagttc tattaagtga tgaagaaagt aaaacttggt tagttataat tgcgattaga
    11821 ctttgtttct agcaacgaga cttcgctata catactttaa aagaacttga aagtatgtat
    11881 agatatataa tcgtgaaaac ttaactattg tttacatgga gaaaaagaat gtatcaagaa
    11941 ttaaatgatt atttagaatt ccaatcaatg aagtatatta tgcctgagaa ggcaggccct
    12001 ttaaaggacg atatgcaaac ttttaaggca aagggccaac ttgctaggaa tagctttaca
    12061 gagttatcaa gagccttaga acaacgtatg gatggcttta aaatgcaaag agtgagtaat
    12121 tgggctaatc aagctcaagt agggcgtcca catttttggg tttattatcg aaaggataca
    12181 gaccaattag atgatgtggc tgttgcgcta agggtttatg gtgtaaaaga tagttttggg
    12241 gttagtcttg aggtaagctt tgtagagagg caaaaatctg ataagacact cgagaagcag
    12301 gctagagtgt tatcgattcc gattgcctct ccattgtatt ttatggtcca aaggcaaggt
    12361 gaaactcatc gagaggaagg caatgaggaa aatcgtcaac gtttaatgca agaaattaag
    12421 tcagggaagg taaggaaagt attggtaaaa tatgatgttc ttttgactga aaaccagagc
    12481 ttagaaaata ttctgcaacg gttgttagaa ggatttgaga aagtgctacc ttactaccaa
    12541 gtttgtcaaa ctattagcta agttcatgga aaaaatgagt tggtatacca actcatttta
    12601 gagtgcctaa tttggtttaa taatatcgat attacccata tatggacgaa gaacttctgg
    12661 gatagtcaca gaaccatctt cattttgata attttcgagg attgctgcta cagtacgtcc
    12721 aactgcaagt cctgaaccat ttaaagtatg aagcaaccta actttgccat caacttcgtc
    12781 acggtaacga atttgagcac gacgtgcttg gaaatcttca gtattagaac atgaagaaat
    12841 ttctcgataa gtattttgag cgggaatcca tacctctaag tcatacgttt tagctgctga
    12901 gaatcccata tctcctgtac acaaggtaat gacacggtat gggagattaa gtttttgaag
    12961 aatattttca gcatttgctg tcattttttc taattcttgg tacgattcct caggcttggc
    13021 aaatttcacc atttctactt tatggaattg atgaaggcga attaatccac gtgtgtcacg
    13081 tcctgcagag cctgcttctg aacggaatga tggactcatg gcagtaaagt atataggtaa
    13141 ttctttccca tcgataattt catcacgata atagtttgtt aaaggaactt cagctgttgg
    13201 gataagtacg aatgggctat ctgctaattc gaaagtatct tccttgaact ttggatattg
    13261 tcccgtaccg aacattgaat cgtggttaac catataagga gggatcactt cagtgtaccc
    13321 ttccttagcg tgttcatcca gcatgaagct gtagatagca cgctctaaac gagcacctag
    13381 acctttgtag aagaggaagc gagaaccagt aacttttgca ccacgttccc aatcgagaat
    13441 tccaaggctt tcccctaaat cccagtgagc tttggcctca aaatcaaatt cacgaggagt
    13501 tccccagcga cgaacttcaa cattttcatc ctcgtctgct ccaagcggca catcatcagc
    13561 tggtgtatta ggaagtacgg tcaccattga ttgtaaatta gcatccacat ctgccaattc
    13621 cgcatcaatt gctttgatat cagcggaaag tgtttgcata gctgtgattt gctcgtctgc
    13681 attctcttta tttcgttttg cttgagcaat ggcagcagaa gcaacattac gttgcgcttt
    13741 cgcctcttca gatttgataa gtaattcacg gcgttttata tcaagttctt taagggttgt
    13801 taatgtttct tggtcaacac cacgcgtggc caatttttta gcgacagcat caaaatctgt
    13861 acgaatacgt tttaagtcta acatatagga atctccttta tactaaaaaa aacaccctgt
    13921 catgctggag cgcaaaggcg cggttccatc cagcttcaca gaagtgcact tgattgattg
    13981 attaaattat ttcccataaa tggaacacgg tagaatttca taaaacaatc ttatctgctc
    14041 gcaacgaccg cagactctct gaaaagaact attatactac ttatccgtta aggttattat
    14101 atccaaaaat aagtaaaatg taaaccttaa ctttataagc ctgctctttg ttttagtgaa
    14161 atagctatgc taaaatatga ttatggaaaa gtatacaagt attaaacaat caggtcgtca
    14221 ttacggctta gaccttttaa ggattatttc catgttcatg attgttatta cgcatgttct
    14281 tggaaaaggt ggtctgagat cttcggttga gggacacgca gactcctatt ttatagtaac
    14341 gtggattatc caagtcttag tttatggggc cgttaactgt tacgcactaa taagtggtta
    14401 tgtgggaatt aattcgcgtt atcgttactc aaaattactg tctatctgga cacaggtttt
    14461 tttctacacc tttacaataa cagcactttt tgcaatcaca ggtcatgaag tgaccttgct
    14521 taattggaga gatgcttttt tccctattgt cagtgggcag tattggtaca ttactgctta
    14581 ctttggtcta ttggttttta tgcctgttat taataatgga ttaaatgcct tgactgataa
    14641 acaattaaaa caattggttt tgttaatgtt tattattttt tcaattttac cagctgtttt
    14701 aaacaatcgt gttccagaat tttcattgag caagggtttt gaaatgactt ggctactgat
    14761 tttatatatt attggtgcct atttgaaacg tattgattta aatatattta aaacatcata
    14821 cttacttata gtttatctat tatcattggt tgctacttat gtcatgaagt ttagtattgg
    14881 tgatatttgg tattggtatg tttcgcctac tttaacttta ggggcggtaa gtttgtttat
    14941 attatttgct agagcaagca ttaagccctc gggattctta aagaaaataa ttgttgtatt
    15001 agccccttca accttgggag tttatctctg tcatctccac ccactaatag taaaatattt
    15061 tgtaagagat tttgcagaga cgtttgttta tgagtccatt tatctttacc catttttaat
    15121 tttaggagca gggattttaa tttatttgtt ggcttttatt gtagaacgta ttagattacg
    15181 aatatttaaa gcaatccatt tttctaactt tttgagatgg tttgatgaca agttcccata
    15241 ttaatattag agttaaaaaa taagatagac tatagtctat cttatttttt aaacttgaat
    15301 atttttaaaa tggtctggat gagggtagga agcttaacaa ctttatcgtc tccaatatgt
    15361 cgtctcgcat gctttaatat cgttccagag tctttagagg caaataaaaa ttttccttgg
    15421 tctgtaaaga cctcaaagtg acgactaatt ttttttcgac taacattagc tccaatttga
    15481 ttgattgatg tccatggaat ttgaatatag tcgttgacat ttttatcgtt atagaattct
    15541 aatgcctggt caccaactaa aaattttcca actttattcc ccatacctaa atacgaaact
    15601 cctgttgttt gaaattcaac agttttgttt aatgattgtg ccatgactaa tcccttttta
    15661 atttatagta gtttgattat atcataatag tgggactcta agtggatttt tggtaataaa
    15721 atataagaga tggaatacta ttgtaaccat ctcttagttt tattacatca cacctgcaag
    15781 gcgagctaga ataccaacga taaacaaacc gatgataatt gtaattggtg atactttctt
    15841 cttaagtaac cacatacaga agaatgtaag aagtaatcca gctaaaccag gaataagtga
    15901 gtcaagttgt ccttgaagtg tttgtggttg cattttatca aggcttaagc cactctgaac
    15961 ttgtcctagg atatcatgca attgttgtcc actaacatgg tctttaggga aattgatgta
    16021 tgccccttct gacaatgtct ttttaggaag gtctacagta aagttaattg ctacccaacg
    16081 cttaacaagg acagcaagaa tgaacatacc aaggattgaa gcacctttag taatatcttg
    16141 gaggataccg ccagacatat ctttagtaat ttctttacct gatttgtaac ctaactcttg
    16201 tgtataccat aagaatgaca tacgaatgag gttccaacca acaaagaaga tgattggtcc
    16261 gaggatatta ccagcagagg caagtgaagc accaagagca cctaagatag gacgtacagt
    16321 aaaccagaat actggatcac caataccagc aagaggtccc atcataccga ttttaacccc
    16381 ttgaattgct ttgtcttcga caggtgtacc gcttgctttt tcttcctcaa gagcaagggt
    16441 tacaccgata attggagcag caacgtatgg atgtgtgtta aagaattcca tatggcgctc
    16501 aagcgcagca gcacgatctt ctttagtagt gtaaagtttt ttcaatgctg ggataagagc
    16561 gtaggcccac cccatatttt gcatacgctc atagttccaa gaaccttgaa ggaattgtga
    16621 acgccaccaa actttttgac gatctgattt tgataattta atttgttcag tcattgttta
    16681 gctcctttct agtagtcctc taagatatcg ccgataggat cgttagaacc tgcgttagaa
    16741 gtacctttgc ttccacccat ttttgataaa ctgatgtaga tgagggcgat agcaacacca
    16801 atagtaccca ttgcaataag agttaactgg ttcaaagctg ctagagcaaa tccaagagca
    16861 aagaaaggcc aaacttcgcg agtagccatc atattgataa ccatagcgta accaacggca
    16921 acaaccatgg caccaccaac ttgcatacca ccatttaacc aatcaggcat tgcttcaaga
    16981 atactttgta ctgaagaaga tggaatagct aaaagaagcg cagctgggaa tgcaatacgt
    17041 aatccttgga gaaggagggc aataaagtga aaacgttcaa ttgctgcgaa gtttccttca
    17101 gaagcagctt tgtcaccagc gtgaacaagt gcagttgaga ttgtacgaac aatcattgta
    17161 aggaaaagtc cagcaatagc tagaggtaca gcagtagaga aggcaaagtt gatacctttt
    17221 tgagtgaagt cgccactttt aaccaaaata atagcagcag caacagaggc aagtgcagcg
    17281 tctggtgcta cggcagctcc gatatttgcc caagcaagag caatcatttg gagactacca
    17341 ccaagaataa tacccgcttc gaggtgacca gttacaagac cgattaaagt acaagcaaca
    17401 agaggttgat ggatttggaa ttggtcgagg ataccttcaa gaccagcaaa gaaggcaacg
    17461 ataacgacca aaatcattga gataattgaa atatccgcca ttttgatatc ctttcgtttt
    17521 cagagataat aagttatttt acgtttgctt tgttgattaa atcaaacaaa ttctttttag
    17581 cgtcatttgg tactttacgt acatcaaatg atacacctaa gtcacgtaat ttttcaaagg
    17641 cagcaacatc gtctttatcc attgaaagaa cgttgtttac cattgtttta ccagttgagt
    17701 gagccattga accaacgttc aattcaggga tttccacacc accttcaact gcacggagtg
    17761 catcttgtac agtttcaaat aagatcaagg cacgtgtgtt tccgaaacgt gggtctttag
    17821 ctacttcaat taatttacta attgggacaa cgtttgcttt gactcctcca ggagcagctt
    17881 gcttaatcaa ttgtttacga agttcatctt tagaaacttc atcagaagca acgataatac
    17941 ggtttgcttt tgatgctgga gtccatgcag ttgcaacttg accatgtaaa agacgtgtgt
    18001 caatacgagc aagattaatt ttaagcttgc catctccaat aacagttcct tcagggatag
    18061 atccactttg tttaacttcc gcaggaacat ctgcaggaac acctgctaca ggagtagctt
    18121 ctacaacagg attaagttct tctggaaggg ctttgatacc ttccttagac tctttaataa
    18181 tatttgcggc aacttgttca acacctgcat tagcgtccat catacgctca gtataggctt
    18241 ggataagcat tggcaaattg agaccagtga tgatagccat tttacgttca ggattttctc
    18301 ccataacacg gcttgcttgg ttaaatggtg acccactcca aaggtcagct aacacaagca
    18361 cctcatcatc agcatcaaat tgtgcgatag cattgttgaa gtgtccatac aaatcatctg
    18421 gtccttcatt tggcatgaaa gttacaactt gaactttttc ttgttcacca aagatcattg
    18481 aacctgattg atgaatacct tcagcaaatt taccatggct ggcaataata ataccgatac
    18541 ccattcttgt tcttcctcct taaaaaattt tttcgttgac ataaagaaga ataatctcct
    18601 tccaatcgta aaattggcca ttctttttca tttcaacgaa attatagaac agatatattt
    18661 taaaacgttt tcagaaaaaa atcaatacta tttctaataa aaattaaaaa aaagatattt
    18721 ttttcacaaa taccttttat aatgggggaa gagagtggta actttttttg ttaataatat
    18781 agtagaaaac ctaaaaaacg atttctatta gaattctgtt tcaatagaga tcgtttttga
    18841 aaatactaca ataataattt ttctagagtt ttagcaacac catcttcttc gtttgtccat
    18901 tggatttgtt ggtcggcata tggaagtaga gttggactag catttttcat agcatatcct
    18961 gtatgtgcaa aggccagcat ctcggtgtca ttgtgttcat ctccaaaggc aatcaaattt
    19021 tcttgtgaaa gattgagtga ttttaataaa tgtttgagag cataagcttt gttaacacct
    19081 ttaggtgaaa attcgagtat attcaatgga ccaccccaag aatctacttc tagttcgtga
    19141 ttaaaatgtt gtcgtaactc tttagctaac tcatacttat ctttatgacg tgtttgcatc
    19201 aatagagcat ttggattgcg tgtgatttta gtaacatcta atgccatttt atcagtaatt
    19261 tcattgacac cgaataattg tgggttaatt ttatcacgat tatccatagt aatgtaaaaa
    19321 tttttacggt actcgctagc aataaaatcg gcttggatag catctgataa atttaaggtt
    19381 tcaaggagta attttttatc aatcgttgca ctatgctcaa acgcccactt tttttcgggg
    19441 atatgagtta aagcaccgtt aaaatttatc attggtgttt ttaaatctag gcgtaaataa
    19501 tgagcgagtg ccatacggta tgggcgtccg gttgtaataa ttactttgtg tccttgagct
    19561 tgaacttttc gaagtgtatc agcggtgtag tcagaaatag tgttattatt atgtaataga
    19621 gtaccgtcta aatctacggc gataattttc tttgtcatgc tgacattata tcaaaaatag
    19681 atttcaataa aaagagacag tagaaatatg atataataac tgtggaaata tggaagttgg
    19741 tggattatga aaaagttaat ttggagtcat ctttttgcca tagtcagtat tttagggata
    19801 tggtattatc atgtaaaaat tgatcatttg atggtaattc tattggcatc agtcttgtcg
    19861 ttagagatgg gattcattgc ttattatagt cgtaatattt ttattcgtct tttagcaagt
    19921 ttaggccttt tattttttta cccacagagt ttagggattt ttcttagttt agtgagctta
    19981 gagtggtcaa caaaagacat ttggcagtct aatcacttta tgcaatatat ggcatacctt
    20041 cttgcaattc tttttattat ctcttcagct ttactaagtt tgaagctatt ggtaaatgca
    20101 tatgatatta gtaaaaggta tcaattagca actgttatac ctgtaatgct ttttagtagt
    20161 tttgcctttt gcttagaaaa atcaggatca ttaggtttag aaggaatcat tttaaatccg
    20221 tcagcatttt ttaatggaat acagactaat atggctaacc ttgattttaa agccttgtct
    20281 tgcctttttt tagtacaatt attactttgg ggaatattaa tggaagacta accacaacag
    20341 ggttagtctt ttaggtttga agtttattta accttaattt aataatatgt tgtataatga
    20401 gatattaatt ggaggttaga atgaaagtaa aactatttgc gatacattta ttttttattg
    20461 ttatttcttt tgggattaaa tattatcatt tgcagggacc aaatttgatt tggaatatga
    20521 ctctagccct aatagcgtta gattttgcat accttactag tttatttaag aaaaaaattc
    20581 ttatcggtct ttttgcactt gcttggtttt ttttctatcc taataccttt tatatgttga
    20641 cggatatcat tcatatgcat tttgtcggag acgttcttta taataagaca aatttaatac
    20701 tttatatctt gtatgtatct agtatcttgt ttggtttttt atcaggtatc gaaagttttt
    20761 ctgtgattat gcgtaaattt agaattagca atatattttt aagatgggga attattggaa
    20821 ttgtatcatt tgtttcaagt tttggtattc atattggtcg ttatgcccgt ctaaattcat
    20881 gggatatctt aacgaagcca caagtggtaa ttaacgaact tttagcggtt ccgagtaggg
    20941 attcattcca ctttatttta ggatttacat ttttacaggt attgtgtctt atttttatgg
    21001 attattctgc agggaaaaag cttgaaaagt gaattatttt ttgttaatat atattaatcg
    21061 ttcgtaaata tacgaacgat taattttttt aaaagtgtaa aataacaaaa aataaaggag
    21121 aatccgattt taaaatggaa aagtttttta agctaaagga acacgggacg acaattcgga
    21181 ctgaaatcac tgctggtcta acgactttct ttgcaatgtc gtacattttg tttgtaaatc
    21241 cagctatttt atctcagact gggatgccag cacaaggagt tttcttagcg acgattattg
    21301 gtgccgttgt agctacatct gttatggctt tctatgctaa ccttccctat gcacaagctc
    21361 ctgggatggg attaaatgct ttctttacat atacagttgt ctttgcattg ggatatacat
    21421 ggcaagaagc cttagccatg gtatttattt gtggtttgat ttctctgatt attactttaa
    21481 cgaaagttag aaagatgatt attgagtcta tcccaacaac tttaaaatca gccattacag
    21541 caggaatcgg aactttttta gcatacgttg ggattaaaaa tgctggtttt ttgaaatttt
    21601 caattgatcc tgggacgtat gatgttgttg gaaagggtgc tgcgaaagga ttggctacta
    21661 ttacagcaaa ttcttcagca acgccagggc tagtgtcgtt tgataatcca gcaattttat
    21721 tatctttgat tggtttaagc attaccatct tctttattgt aaaaggtatc cgtggaggga
    21781 ttattctatc gatattaaca acgaccctct taggtatttt aatgggagtg gttaagttag
    21841 atgctattaa ttgggaagca actaacctct cggcctcttt tagagatttg aagcaagtat
    21901 ttggagttgc attaggagag aaaggattaa tttctttatt ttctaatcct tcacgtctac
    21961 caagtgtatt aatggccatt cttgcttttt cactgactga tatttttgat accattggaa
    22021 cgctcattgg taccggagaa aaagttggta tcttagcaac gactggtgat aatcatgaat
    22081 ctaagagttt agacaaagca ctctattcag acttaattgg gacaactttt ggtgctattt
    22141 gtggaacatc taacgttaca acctatgttg agtcagcagc aggaatcgga gctggtggtc
    22201 gtacaggatt gacagcctta gtagttgctg gtttgtttgc tatatctagt ttttttagtc
    22261 cactagtatc tatcgttccg agtcaagcta ctgcaccaat attggttatt gttggtatta
    22321 tgatgctttc aaatttaaaa gatatcaaat gggatgatat gtcagaagct atcccagctt
    22381 tctttacttc tctttttatg gggtttactt atagcattac ttatggtatt gcagctggtt
    22441 tcttgaccta tacactagct aaagtcatca aaggacaagc aaaagatatt catgtggtac
    22501 tgtggatttt agatatatta tttattctaa actttatcag cttggctatt ttgtaatatt
    22561 atagtttgat aaataagata aaaggaaatc agatttgtag cctggtttcc ttttttctta
    22621 agaaaagtga agaaatatat gctatggggc tgtcagaatc agcaaactgt tggaagacag
    22681 agatatttct ggtataatat ctctatgttt tatactcaaa atgaagaaga gcttattgcc
    22741 ctagggcaaa aactgggtac tgttttaaag tcaggagata tcgtattatt gactggtaat
    22801 ttaggtgcag gcaaaacaac tcttacaaaa ggtattgcaa agggattaga cataaaacaa
    22861 atgattaaga gcccaactta cactattgtt agagaatatg aagggcgtgt accactttac
    22921 catttggatg tttatcgtat tggagatgat cctgattcta ttgacttaga tgacttcttg
    22981 tttggtcaag gagtaacagt tattgaatgg ggagagttgc ttagtgataa ccttatcaat
    23041 aactacttag aaattgttat tacgaggagc aatcaaggac gtcaagttca gttagaagct
    23101 tatgggcatc gcgcaagaga aattattgag gcgattcaag atgtctaaac aatttgctgt
    23161 tgattttata gaagcttcga gagaggaagc atctgcgctt cttgaatttt taaatactgt
    23221 tactgaggaa acagatttta ttttgcatac ggtgagcaat caactatctt tgccagaaat
    23281 ggaaacgttt attgaaaata ccttaataac gaaaaattgc atttgtttga ttgctaaatt
    23341 aaaaaataaa gtgatcggct tgataacaat cattagtcag tctgatattg aaattgaaca
    23401 cattggggat ctttttattg ctgttcagaa ggattattgg gggtatggta ttggtcatat
    23461 tctcatggaa gaagcaatag aatgggcgtc agataacgat attacacgcc gcttagaact
    23521 atcagtccaa ggacgtaatg aacgtgctat tcacctctat caaaaattca gttttgaaat
    23581 agatgggctt caaacaagag gtattaaaag agaaaatgga gaatttctag acatttacag
    23641 aatgagtaag ttgatagatt aggaatatac atgaaaattt ggaaaaaaat aaccttaatg
    23701 ttttctgcaa ttattttaac aacagtaatt gcattgggag tctatgttgc ctcagcttat
    23761 aatttttcga ctaatgaatt gtctaagact tttaaagatt ttaagctagc taaatcaaaa
    23821 agtcatgcta ttgaagaaac aaagccgttt tcaatactat taatgggtgt ggacacaggt
    23881 tcagagcatc gaaaatctaa gtggtcagga aatagcgatt ctatgatctt agtcactata
    23941 aatcctaaaa ctaataaaac aacgatgaca agcttagaac gtgacgtatt gattaaattg
    24001 agtggtccca aaaataatgg acagactggc gtagaagcaa agctaaatgc agcctatgct
    24061 tctggtggtg cggaaatggc attgatgact gttcaagact tattagatat taatgttgat
    24121 tactttatgc aaattaatat gcaaggatta gttgatttgg tcaatgctgt tggtggtata
    24181 acagtaacta ataaatttga ctttccaata tcaattgctg ccaatgaacc agagtacaag
    24241 gctgttgttg aaccagggac acataaaata aatggagaac aagcacttgt ttattctcgt
    24301 atgcgctatg atgatccaga gggagattat gggcgtcaaa aaagacaacg tgaagtaatt
    24361 caaaaagtcc ttaaaaaaat attggcgtta aatagtatta gttcatacaa aaaaattctt
    24421 tccgcagtaa gtaataacat gcaaactaat attgagatat catcaaaaac gattcctaat
    24481 ttgttagctt ataaagattc attggaacat attaaatctt atcagttgaa gggtgaagac
    24541 gctactctat cagatggtgg ctcttatcaa attttaacta agaaacatct acttgcagtt
    24601 caaaatagaa ttaagaaaga gctggataaa aagcgtagta aaactctgaa gacaagcgcg
    24661 attctatatg aagattacta tggtactact gctagtaatg attcttctac ttattcatca
    24721 acacaagaga ataattataa tacaacacct tattcagaag caccaccaag ttacagtggt
    24781 aatactactt atagttctga gactaatcaa acaactcatc aaagttacta taatagtagc
    24841 actcctgcta gtaactatag cagtaacact aacacaggtc aggctgattc aagtggaagt
    24901 gttaataatt ataacggggc tgcaacgcct aatccaaaca caggaacgca accagtacca
    24961 agtcaaacta atccataaaa tataaaaaag agcattattt gctcttttta tatttagagt
    25021 agatattttt ataccgtcgg cttattcgtc tttctcttga tatttagcca tgcgttgatt
    25081 aatttcttct aaacgaggtt gaat
//
LOCUS       DATRUK010000026        24332 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.026, whole genome shotgun sequence.
ACCESSION   DATRUK010000026 DATRUK010000000
VERSION     DATRUK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24332
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.026"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(34..744)
                     /locus_tag="VBE34_001016"
     CDS             complement(34..744)
                     /locus_tag="VBE34_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7263744.1"
                     /translation="MNIKRHSIFLWLTTSILAILMAVGMTIYFNTYQASPLARRIAKT
                     ATNHKNYLLFRSKGKVKANIIFYQGALVEEEAYSQLARDLADKGYNTYILKTPLNLPV
                     LSPYKAKTIINQNHLTNVYLAGHSLGGVVASQNAKVAPVRGLILLASYPSSKSDLSHK
                     KLRVLSITASNDHILNWEKYEEAKKRLPNSSTFRTIVGGNHSRFGNYGHQKGDGKATL
                     SHKSSEKQLATFISNFIH"
     gene            complement(741..1439)
                     /locus_tag="VBE34_001017"
     CDS             complement(741..1439)
                     /locus_tag="VBE34_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001907086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO7263745.1"
                     /translation="MPFIFRHSFFNKVLIFWYTIIMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDTFDNPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIINVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPSHYSVHPGHGMN
                     TTIGHEKNFNPFFQ"
     gene            1607..2371
                     /locus_tag="VBE34_001018"
     CDS             1607..2371
                     /locus_tag="VBE34_001018"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEO7263746.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGVLDYNEETAQAAVD
                     KLSLEDAVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGIAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            2479..4986
                     /locus_tag="VBE34_001019"
     CDS             2479..4986
                     /locus_tag="VBE34_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEO7263747.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLVKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEQLFLEGFELRTPRIDTVELSQVFYPCLEKYSLGALAESLNIEL
                     TDAHTAIADARATAQLFIKLKAKISSLPKEVLETILTFADNLLFESYLLIEEAYQEAD
                     FVNPKEYYFWQGLVLKKEKAVGKPKKLSSDFQVNMALLGMDARPKQIVFADLVKAHFN
                     DQTTTFLEAQSGLGKTYGYLLPLLDQSQKQQIIVSVPTKILQDQIMAKEIKHIQELFH
                     IPCHSIKGPRNYLKLDAFYKSLQVQDRNRLINRFKMQLLVWLTETTTGDLDEIKQKQR
                     LESYFDQLKHDGEVTQSSLFYDLDFWKRSYDKVAQSQLVIINHAYFLERVQDDKDFAK
                     GKVLVFDEAQKLVLGLENFSRGQLDISHQLQVIQKIIDSPIPLLQKRLLESISYELSH
                     AAELFYRHNSFEFSETWLKRLKNSINALEVVGLDDLQTFFTATYTNYWFETDKVNEKR
                     LTILRGAREDFLKFSEFLPPTKKTYMISATLQISPKVYLSDLLGGFSSISTEKIAHEK
                     NANQKVWIDTSMPNILDLSPEQYAYEIAKRLQDIMTLKQPTLVLLTSKQTMFMVSDYL
                     DKWEIKHLTQDKNGLAYNVKKRFDRGESNLLLGTGSFWEGVDFVHRDRLIEVITRLPF
                     DNPKDYFIQKLSQSLTKEGKNFFYDYSLPMTVLKLKQALGRTTRREEQKSAVIILDSR
                     LVIKSYGQTIMHSLGRDFEISKEKINKVLTEMAKFLI"
     gene            5072..6265
                     /locus_tag="VBE34_001020"
     CDS             5072..6265
                     /locus_tag="VBE34_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7263748.1"
                     /translation="MTYLSERVLNMEESVTLAAGAKARELRVQGRDILSLTLGEPDFA
                     TPKNIQQAAIEAITDGRASFYTPSSGLPELKSAINAYFERFYGYSLKPNQVVVGTGAK
                     FILYTFFMTVLNPGDEVIIPTPYWVSYADQIKMAEGKPVFVTAKEVNHFKVTVEQLEA
                     VRTDKTKVILLNSPSNPTGMIYKAEELEAIGNWAVEHDILILADDIYGRLVYNGNIFT
                     PISSLSESIRNQTIVINGVSKTYAMTGWRVGFAVGNHDIIAAMSKVVSQTTSNLTAVS
                     QYATIEALNGSQESFEKMRLAFEERLNIIYPLLCQVPGFEVVKPQGAFYLFPNVTKAM
                     EMKGYTDVTAFTDAILEEVGLALVTGAGFGAPENVRLSYATDLGTLKEAVRRLHVFMG
                     SNEIN"
     gene            6286..7632
                     /gene="asnS"
                     /locus_tag="VBE34_001021"
     CDS             6286..7632
                     /gene="asnS"
                     /locus_tag="VBE34_001021"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEO7263749.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEVIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYSTYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYIAEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIKP"
     gene            complement(7672..8229)
                     /locus_tag="VBE34_001022"
     CDS             complement(7672..8229)
                     /locus_tag="VBE34_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7263750.1"
                     /translation="MTKSPARLISFISIAIAINLIGANLALFLRLPIYLDTIGTLLIA
                     VILGPWYAASTAFLSALINWMTTDIFSLYYSPVAIVVAIITGILIKRNCKPSSLLWKS
                     LIISLPGTIIASVITVILFKGITSSGSSIIAQFLHGIGLDMTSSLILVQVGTDYIDRL
                     ISLILVFSTITLLKKHSPNLINQRF"
     gene            complement(8226..9209)
                     /locus_tag="VBE34_001023"
     CDS             complement(8226..9209)
                     /locus_tag="VBE34_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEO7263751.1"
                     /translation="MNKEKIIIDCDPGIDDTLALMYAIQHPKLEVVAITITAGNSPVE
                     LGLKNTFVTLELLNRRDISVYVGNNLPLQREFVSAQDTHGMDGLGENNFTLAQPIIFQ
                     EESADCFLANYFEHKNDTSIIALGPLTNIARALQTNPKLGKHCKRFVSMGGNFKSHGN
                     CSPVAEYNYWYDPHAAQYVFENLDKKIEMVGLDITRHIVLTPNHLSYMERINPDVSTF
                     IQKITKFYFDFHWQYEHIIGCVINDPLAIAYFVNENIATGFDSYTDVACHGIAMGQTI
                     VDQYHFYKKDANSKILTSVNTNLFWNDFMTVLLHAQNSVIIEDLEMLGLNK"
     regulatory      complement(9353..9397)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            complement(9455..9868)
                     /locus_tag="VBE34_001024"
     CDS             complement(9455..9868)
                     /locus_tag="VBE34_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEO7263752.1"
                     /translation="MLYEVTSSNTQGVDGKVYLSNGKIVETNHPLNHLPGFNPEELIA
                     LAWSTCLNATIKAILEQKGFKDLKSRVDVNCQLMKEKQVGKGFYFQVNAVASIEKLSL
                     SDSKLIVNKAHSRCPISKLISNAKTINLDTVKWSV"
     gene            10022..10912
                     /gene="rapZ"
                     /locus_tag="VBE34_001025"
     CDS             10022..10912
                     /gene="rapZ"
                     /locus_tag="VBE34_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEO7263753.1"
                     /translation="MSDEQIKLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPTLVPK
                     FLELAAQSGDTSKIAMVVDMRSRLFFREINSILDSLEINDNINFKILFLDATDTELVS
                     RYKETRRSHPLAADGRVLDGISLERELLAPLKSMSQNVVDTSELTPRQLRKVISEEFS
                     NQDSQSSFRIEVMSFGFKYGIPLDADLVFDVRFLPNPYYKPELRDKTGLDTEVYDYVM
                     SFDESDDFYDHLLALIKPILPGYQKEGKSVLTVAIGCTGGQHRSTAFAHRLSEDLKAD
                     WTVNESHRDKNKRKETVNRS"
     gene            10909..11883
                     /locus_tag="VBE34_001026"
     CDS             10909..11883
                     /locus_tag="VBE34_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEO7263754.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLRLEDVEITAVVTVADDGGSSGE
                     LRSVMQLTPPGDLRNVLVALSDMPKFYEQIFQYRFAEGDGDFAGHPLGNLIIAGVAEM
                     QGSTYNAMQSLTQFFHTTGKIYPSSEHPLTLHAVFKDGHEVVGESQIADYKGMIDHVY
                     VTNTYNEETPTASRKVVDAILESDMIVLGPGSLFTSILPNLVIPEIKQALLETRAEVA
                     YVCNIMTQRGETEHFTDADHVEVLKRHLGQDAIDTVLVNIEKVPESYMENNHFDEYLV
                     QVEHDFSGLRKHARRVISSNFLKLEKGGAFHHGDFVVEELMNLVGTHL"
     gene            11880..12791
                     /gene="whiA"
                     /locus_tag="VBE34_001027"
     CDS             11880..12791
                     /gene="whiA"
                     /locus_tag="VBE34_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEO7263755.1"
                     /translation="MSFTVKVKEELLGHKSENKMELSAIIKMSGSLGLANHGLNLSIT
                     TENAKIARHIYSMLEEHYHLQPEIKYHQKTNLRKNRVYTVFIEEKVDVILADLKLADA
                     FFGIETGIEHSILDNDENGRAYLRGAFLSTGTVREPDSGKYQLEIFSVYLDHAQDLAN
                     LMKKFMLDAKVIEHKHGAVTYLQKAEDIMDFLIVIDAMEARDAFEEIKMIRETRNDIN
                     RANNVETANIARTITASMKTINNIIKIMDTIGFDALPSDLRQVAQVRVAHPDYSIQQI
                     ADSLETPLSKSGVNHRLRKINKIADEL"
     gene            12806..14203
                     /locus_tag="VBE34_001028"
     CDS             12806..14203
                     /locus_tag="VBE34_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEO7263756.1"
                     /translation="MACTTILVGKKASYDGSTMIARTEDSVNGDFTPKKLKVMTSKDQ
                     PRHYKSVLSNFEVDLPDNPLPYTSVPDALGKDGIWGEAGINSKNVAMSATETITTNSR
                     VLGADPLVSDGIGEEDILTLVLPYIQSAREGVERLGAILEKYGTYESNGIAFSDTEEI
                     WWLETIGGHHWIARRVPDDVYVTNPNQLGIDHFEFNNCDDYMCSSDLKEFIEQYHLDL
                     TYSNEHFNPRYAFGSQRDKDRHYNTPRSWAMQRFLNPEIEQDPRSLFIPWCQKPYRKI
                     TVEDIKYVLSDHYQDSVYDPYGPEGDAVSRRAFRSVGINRTSQTSILQLRPNKSLETT
                     GVQWLSYGSMPFATMVPLFTQVETVPDYFSNTTKDASTDNFYWTNRLIAALADPHFYQ
                     HEADIESYIERTMAQGHAHINGIDREVAENKEIDFQQKNQEMSDYIQKESQELLNRIL
                     FDASNLMTNRFSMGD"
     gene            14345..15865
                     /locus_tag="VBE34_001029"
     CDS             14345..15865
                     /locus_tag="VBE34_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEO7263757.1"
                     /translation="MRKKFLLLISFVAMFAAWQLVQVKQVWADSKLKVVTTFYPVYEF
                     TKNVVGDKADVSMLIKAGTEPHDFEPSTKNIAAIQDSNAFVYMDDNMETWAPKVAKSV
                     KSKKVTTIKGTGDMLLTKGVEEEGEEHEGHGHEGHHHELDPHVWLSPERAISVVENIR
                     NKFVKAYPKDAASFNKNADAYIAKLKELDKEYKNGLSNAKQKSFVTQHAAFGYMALDY
                     GLNQVPIAGLTPDAEPSSKRLGELAKYIKKYNINYIYFEENASNKVAKTLADEVGVKT
                     AVLSPLEGLSKKEMAAGEDYFSVMRRNLKVLKKTTDVAGKEVAPEEDKTKTVETGYFK
                     TKDVKDRKLTDYSGNWQSVYPLLQDGTLDPVWDYKAKSKKDMTAAEYKKYYTAGYKTD
                     VESIKIDGKKHQMTFVRNGKSQTFTYKYAGYKILTYKKGNRGVRYLFEAKEKDAGQFK
                     YIQFSDHGIKPNKAEHFHIFWGSESQEKLFEEMENWPTYFPAKMSGREVAQDLMSH"
     gene            complement(15978..16238)
                     /locus_tag="VBE34_001030"
     CDS             complement(15978..16238)
                     /locus_tag="VBE34_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEO7263758.1"
                     /translation="MKKDIHPDYRPVVFLDTTTGYKFLSGSTKSTKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAQ"
     gene            complement(16347..17282)
                     /locus_tag="VBE34_001031"
     CDS             complement(16347..17282)
                     /locus_tag="VBE34_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEO7263759.1"
                     /translation="MIIFQQILDKIKEYDTIIIHRHMRPDPDALGSQIGLRDIIRHNF
                     PKKKVLATGFDEPTLAWIAKMDQVTDQDYQGALVIVTDTANTPRIDDERYKKGDFLIK
                     IDHHPNDEVYGDLSYVDTNASSASEIVTDFALSCDLLLSTSAARVLYNGIVGDTGRFL
                     YPATTSKTLKIASKLREFDFDFSAMARQMDSFPFKIAKLQGFIFEQLEIDKNGAACVT
                     LTQEDLKRFDVTDAETAAIVGIPGKIDIVESWAIFVEQSDGHYRVRLRSKSHIINEIA
                     KRHNGGGHPLASGANSYSLEENQAIYQEIQEVLSL"
     gene            17548..18570
                     /locus_tag="VBE34_001032"
     CDS             17548..18570
                     /locus_tag="VBE34_001032"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEO7263760.1"
                     /translation="MTQAVLKELAKAELHCHLDGSLSLPAIRKLANMADIILPNSDKE
                     LRKYVIAPAQTESLVDYLKTFEFIRPLLQTKEALRFAAYDVARQAALENVIYIEIRFA
                     PELSMDKGLTASDTVFAVLEGLADAQKEFNIVARALVCGMRQSSHKTTKEIIKHIVDL
                     APKGLVGFDFAGDEFSYPTDSLVDLIQEVKRSGYPMTLHAGECGCAKHIADSLNLGIK
                     RMGHVTALTGQRALIKRFVEEDAVAEMCLTSNLQTKAASSIQSFPYQELYDAGGKITI
                     NTDNRTVSDTNLTKEYSLFVTYFGTKIEDFLVFNQNAVKASFTSDSEKDTLLHKLQEN
                     YDSYLK"
     gene            18629..19072
                     /locus_tag="VBE34_001033"
     CDS             18629..19072
                     /locus_tag="VBE34_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEO7263761.1"
                     /translation="MALAKIVYASMTGNTEEIADIVADKLRDLGLDVEVEECTMVDAA
                     DFEDADIAIVATYTYGDGDLPDEIVDFYEDLAEVDLSGKVYGVVGSGDTFYDYFCKSV
                     DEFEAQFALTGAQKGADCVKVDLAAEDEDIENLEAFAEEIASKLN"
     gene            19149..19424
                     /locus_tag="VBE34_001034"
     CDS             19149..19424
                     /locus_tag="VBE34_001034"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEO7263762.1"
                     /translation="MELEEIRQEIDEIDQQLVSLLETRMGLILEVIAFKKKHRLPVLD
                     NNRENEVLNNVLKKVQNHQFDDVIRATFKDIMTESRVYQKENIVDGD"
     gene            19417..20613
                     /locus_tag="VBE34_001035"
     CDS             19417..20613
                     /locus_tag="VBE34_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEO7263763.1"
                     /translation="MIKRFDNYAQYVLVLGFSLLIGGVVGAITAVFGRVLLFLTAFRS
                     DYIAYLLPFLSIVGLFIVFVYQKFGGKSVKGMGLVFEVGHGNEETIPKRLVPLVILTT
                     WLTHLFGGSAGREGVAVQIGATVSHYFQKYCRLQNASQLFLVMGMAAGFAGLFQTPLA
                     ATFFAIEVLVVGRLMVSYILPSLIAALTANFVSHSLGLEKFSHSIATSMALTPDIILK
                     LLVLGLCFGLCGNLFAYLLAKAKLIASSRLLNPYKRIFTLGLLATFLLFIFHFGRYSG
                     LGTNLIEASFTNKNLYDYDWLLKLCLTVITLAAGYQGGEVTPLFAIGASLGVIIAPIL
                     GLPVILVAALGYTSVFGSATNTLLGPILIGGEVFGFANTPYFVIVCLVAYSISHAHTI
                     YGAQSR"
     gene            21197..21544
                     /gene="rplS"
                     /locus_tag="VBE34_001036"
     CDS             21197..21544
                     /gene="rplS"
                     /locus_tag="VBE34_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEO7263764.1"
                     /translation="MNPLIQSLTEGQLRSDIPEFRAGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            21655..21726
                     /locus_tag="VBE34_001037"
     tRNA            21655..21726
                     /locus_tag="VBE34_001037"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21688..21690,aa:Arg,seq:cct)
     gene            complement(21689..22303)
                     /locus_tag="VBE34_001038"
     CDS             complement(21689..22303)
                     /locus_tag="VBE34_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7263765.1"
                     /translation="MKFLETNEYLRLIKICKEKSQHQSYAALYLIAKTGMRFAECLGL
                     TVNDIDYTNKYLSINKTWDYHFNQRYLPTKNKSSIRNIPIDNDTLFFLHEFTKNKNDR
                     LFDKLSNNAVNKTIRKITGREVRVHSLRHTFASYLISISQVLDHENLNITLEVYAHQL
                     QEQKDRNDKLNQRNLGRIWGKIALNRYLHAMNMSPAGIEPAITP"
     gene            complement(22306..22572)
                     /locus_tag="VBE34_001039"
     CDS             complement(22306..22572)
                     /locus_tag="VBE34_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001881173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEO7263766.1"
                     /translation="MAYFRKRDNGWEYRISYKHIIGNILLCNITSTKYQQVINHYSQT
                     HVQDTVKRFNIHVKACVSMAVHEGYIEKNFCLFTKVKAKNKGRK"
     gene            22572..22976
                     /locus_tag="VBE34_001040"
     CDS             22572..22976
                     /locus_tag="VBE34_001040"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO7263767.1"
                     /translation="MIYSTLAKEQGVQGYLDGKGSLRDICKWYDISSRSVLQKWIKRY
                     TSGEDLKATSRGYSRMKQGRQATFEERVEIVNYTIAHGKDYQAAIEKFGVSYQQIYSW
                     VRKLEKNGSQGLVDRRVKGLESRPDLTEIEQL"
     gene            23138..23338
                     /locus_tag="VBE34_001041"
     CDS             23138..23338
                     /locus_tag="VBE34_001041"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7263768.1"
                     /translation="MPIQALCQLLKGSRSGYYKWLNRQKTDFETKNTKLMAKIKELHR
                     LYNGILGYRRMTTFINRQLGTT"
     gene            23363..23620
                     /locus_tag="VBE34_001042"
     CDS             23363..23620
                     /locus_tag="VBE34_001042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7263769.1"
                     /translation="MNILGISSVIRRVSHACTKAGDRFYEENILNREFTATAHNQKWC
                     TDVTYLQYGLGAKAYLSAIKDLYNGSIIAYEISHNNEIHLL"
     gene            23746..23955
                     /locus_tag="VBE34_001043"
     CDS             23746..23955
                     /locus_tag="VBE34_001043"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7263770.1"
                     /translation="MSRIGKCIDNAPTESFFGFFKTESYHLKKYNSYDELVNDVARYI
                     EFYNTQRYQSKLNNLTPLEFRNQVA"
     gene            complement(24128..24289)
                     /locus_tag="VBE34_001044"
     CDS             complement(24128..24289)
                     /locus_tag="VBE34_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEO7263771.1"
                     /translation="MGGIGAHKFYAGKIGQVFLYIIFSITFIPGIIDLIEALMALGKQ
                     TDEYGNIIV"
BASE COUNT         7843 a         3873 c         4843 g         7773 t
ORIGIN      
        1 tttcatctct ttttattaat tgtagatatc atgctagtgg ataaaatttg aaataaacgt
       61 agccaattgt ttttcagatg atttgtgaga aagggtagct ttaccatccc ctttttgatg
      121 tccgtagtta ccaaatcggc tatggtttcc cccgacaata gttcgaaaag tacttgaatt
      181 tgggaggcgt tttttagctt cctcatattt ttcccagttt aaaatatgat cgttacttgc
      241 tgtaatggag agtactctta actttttatg agataggtcg gatttacttg aagggtagga
      301 agctaataat atgagccctc taacaggggc tactttggca ttttggctag caacaactcc
      361 tcctaaagaa tgtccagcaa ggtaaacatt agttaagtgg ttctgattaa taattgtttt
      421 agctttataa ggtgataaaa ctggcaaatt taaaggtgtc ttcaaaatat aggtattata
      481 ccctttatca gctaaatctc tagcaagttg agaataagct tcttcttcca caagagcccc
      541 ttgatagaaa ataatatttg cttttacctt acccttgctt ctaaaaagaa ggtagttctt
      601 atggtttgta gcggttttag caattctcct agctaatggg gaggcttggt aagtattgaa
      661 gtaaatggtc atcccgacgg ccattaaaat agcaaggata cttgttgtta accaaagaaa
      721 aatagagtga cgttttatgt tcattggaag aaaggattaa agtttttttc gtggccaata
      781 gttgtattca tgccatggcc tgggtgaaca ctataatgac taggtaaggt gaatagttct
      841 tggcgaatac cagtaatgag gtcctcaaag ttactggtag gtaagtcagt acgaccgata
      901 gtttcacgaa atagagcatc gccagtaacg actaattcat ctgagtggaa aacaaatgag
      961 actcctcccc aagaatgacc aggtgttggt aaaactgtga attcaaaacc atttagttga
     1021 tagggttgct ttaatttaaa aaagttctct gccggtcttg ctataacatt aatgatatca
     1081 tcatgacggc ctaacccaga taaattatca tctggggaag atagccaagc ggcttctttt
     1141 tctgaaacat aaacaggtgg attatcaaaa gtatctctga ccaaatctaa actaaaaata
     1201 tgatcatagt gagtatgagt taagaggata gccactaaag gtttttcaaa ggatttaatt
     1261 ttagcaatga tttcttggcc attgctccct ggatcaatga ggataacagc ttgatcatta
     1321 acgaggtaat aagtattttc accagcaatg tggtttattg ttttatagat tttcataatg
     1381 atagtgtacc aaaaaatcaa gactttgtta aaaaaagagt ggcggaaaat aaatggcaac
     1441 tagtttcaga aaatatggga tctcacagca atttcttgac accgttttca taaaggtgta
     1501 tcataaaagt aatcaataag ccaagcctaa agctgaactt agtttaagaa cactgacttt
     1561 ttgagagcaa cctaattggt tgactaaaga atatgaggtg gaagatatgt caaaagttgc
     1621 tattgttact ggtgctggtc aaggaattgg atttgctatt gcgaaacgtc ttcatgcaga
     1681 cggctttaaa ataggcgttt tagactataa tgaagagact gctcaggctg cggttgataa
     1741 actatcactt gaggatgctg ttgcagttgt agcagatgtt tcaaaacgtg atcaagtctt
     1801 tgatgctttc caaaaagttg tagatacttt tggtgatttg aatgttgttg ttaacaatgc
     1861 aggtattgcc ccaacaaccc ctcttgacac tattactgaa gagcagtttg aaaaggcttt
     1921 tgcaatcaat gtcggaggaa ctatttgggg aagtcaagca gctcaaaaac attttagaga
     1981 attaggtcat ggcggaaaaa ttataaatgc aacttcacaa gcaggatgtg aagggaatcc
     2041 taatttgact gtttatggtg gtacaaaatt tgcagttcgt ggtattacac agactttggc
     2101 aaaggatttg gcatcagaag gaatcacagt caatgcctac gcaccaggta ttgttaaaac
     2161 acctatgatg tttgatattg cccatgaagt tggtaagaat gcaggcaaag atgatgaatg
     2221 gggtatggag cagtttgcaa aagatatcac tttgaaacgc ttgtctgaac cagaagatgt
     2281 tgctaatgca gtaggcttct tagcgggaga tgattcaaat tatatcactg gacaaacaat
     2341 agttgtcgat ggtggtatgg tattccatta aaaatgatta atgctaacac tgtgagtgaa
     2401 actcacagtg ttttttatat ttcttaaaaa attatcttat cttggttatt ggagtgatgg
     2461 catctttgag gtacagtcat gttttgtttt atagatatag cgtgctataa tagacttact
     2521 atgacccaga aaaaacttag aaaatatgca gtagttgact tggaggctac tggagcaggt
     2581 cccaatgctt ctattattca ggttgggatt gttattatcc aaggaaataa aattattgat
     2641 agttatgaaa cagatgttaa tcctcatgaa tctttggatg agcatattgt tcatttgaca
     2701 ggtattacgg ataagcagtt ggttaaagct ccggattttg gtcaagtggc gcatcacatt
     2761 tatcaattga ttgaagattg tatttttgtt gcccacaatg taaagttcga tgcaaattta
     2821 ttggctgagc aactattttt agaaggcttc gagttacgta caccaaggat agatacagta
     2881 gagttatcac aagtgtttta tccctgttta gaaaaatatt ctttaggggc attagcagaa
     2941 agcttaaata ttgaattaac agatgcgcac actgctattg cagatgcaag agctacagca
     3001 cagttattca ttaagttaaa agctaaaatt agtagtttac ctaaagaagt gttggagacc
     3061 attttaacat ttgctgataa tcttttgttc gaatcctatt tattgattga agaagcttat
     3121 caagaagctg atttcgttaa tcctaaagaa tattatttct ggcaaggatt ggtcttaaaa
     3181 aaagaaaaag ctgttggaaa gcctaagaaa ttatctagtg attttcaagt taacatggct
     3241 ttgttaggta tggatgctcg ccctaagcaa attgtttttg ctgacttggt taaagcgcat
     3301 tttaatgatc aaacaacaac gtttttagaa gctcaatcag gacttggtaa aacatatgga
     3361 tatctcttgc ctttattgga tcagtctcaa aaacaacaaa taatagtatc tgttccaaca
     3421 aagattttgc aagatcaaat aatggctaaa gaaataaagc acattcaaga actttttcat
     3481 attccctgtc acagtatcaa aggaccgaga aattacttga agttagatgc gttttataag
     3541 agcctccaag tgcaggatcg aaatcgattg ataaatcgtt ttaaaatgca actgctagtg
     3601 tggttgacgg aaacgactac aggtgatttg gatgaaatta aacaaaaaca acgattagaa
     3661 tcttactttg accagctaaa acacgatgga gaagtgactc aatcatcact tttttatgat
     3721 ctggattttt ggaaacgcag ttatgataaa gttgcacaaa gtcaacttgt tattattaat
     3781 catgcttatt ttcttgagcg tgttcaggat gacaaagatt ttgcaaaggg gaaggtttta
     3841 gtttttgatg aagctcagaa acttgttttg ggactggaaa atttttctcg tgggcaactt
     3901 gatattagtc accaattgca ggttattcaa aaaataattg atagtcctat tccgttatta
     3961 caaaagcgcc ttctggaaag tatttcttat gagttaagtc atgcagcaga acttttttat
     4021 cgtcataatt cttttgaatt ttcagagacc tggttgaaac gtctaaaaaa tagtataaac
     4081 gctttagaag tagtaggatt agatgattta caaacttttt tcacagctac ttatactaac
     4141 tattggtttg aaactgataa agtcaatgag aagcgtttaa ccattttacg tggggcgaga
     4201 gaagattttc ttaaattttc agagttcctc cctcccacaa aaaagaccta tatgatatca
     4261 gctactttac aaatcagtcc caaagtctac ttgtcagatc ttttaggagg attttcaagt
     4321 ataagcacag agaagatagc gcatgagaaa aatgccaatc agaaagtatg gattgatacc
     4381 tctatgccta atatacttga tttatcaccg gaacagtatg cttacgaaat agccaagcgt
     4441 ttacaagata taatgacgtt aaagcagcct accttagtct tattaacttc taaacagaca
     4501 atgtttatgg tatctgatta tttggataag tgggagataa aacatcttac tcaagataaa
     4561 aatggcttag cttataacgt taaaaaacgt tttgatcgtg gagaaagcaa tcttctttta
     4621 ggtacaggat ctttttggga aggtgttgac tttgtacatc gtgatcgtct tattgaagtt
     4681 atcacgcgat tgccttttga taatcccaaa gactatttta tccagaaact atcgcagagt
     4741 ttaacaaaag aggggaaaaa cttcttttat gactattctt taccaatgac tgtcttaaaa
     4801 ttaaaacagg ccttgggacg tactacaagg agagaagaac aaaagtcagc ggtaattatt
     4861 ttggatagca gattggttat taaatcttat ggacaaacca tcatgcattc tcttggtaga
     4921 gattttgaaa tttctaagga aaaaattaac aaggtcttga ctgaaatggc taaatttttg
     4981 atataatggt aaagatcatt gtctaaaaat aagatgttga cagtgcgact caagattaaa
     5041 taatagtagt acgttgataa aaggaggagt gatgacctat ttgtcagaac gtgtactcaa
     5101 tatggaagag agtgttactt tagctgcggg agctaaagca agagaactca gagtacaagg
     5161 aagagatatt cttagtttaa ctttagggga gcctgatttc gctactccta aaaacattca
     5221 gcaagcggct attgaggcta taacagatgg acgtgcaagt ttttacacac catcaagtgg
     5281 tttgccggag ttgaagtcag caattaatgc gtattttgaa cgtttttatg gctactccct
     5341 taaacctaat caggtggttg tgggaactgg tgctaagttt atcctttata ctttctttat
     5401 gacagtactg aatcccggtg atgaagtaat tattccgact ccttattggg tatcttatgc
     5461 tgatcagatt aagatggctg aaggtaagcc agtttttgtc acagcaaaag aagttaatca
     5521 cttcaaagta actgtggaac agttagaagc tgttaggact gataagacaa aagtcatact
     5581 tcttaatagt ccatcaaatc ctacaggtat gatttataaa gctgaggagt tggaggctat
     5641 tggaaattgg gcagtcgaac atgatatcct aattttagca gatgatattt acggtcgatt
     5701 ggtttacaat ggcaatattt ttacccctat ttcaagcctt tcagagtcca ttcgaaacca
     5761 aacgatagtc ataaatggag tttctaagac ttatgcgatg acaggttggc gtgttggttt
     5821 tgcagtgggt aatcatgata ttatagcggc aatgtctaaa gtagtcagtc aaacaacttc
     5881 taaccttacg gctgtctcac agtatgctac tattgaggct ttaaatggtt ctcaagagtc
     5941 atttgaaaaa atgaggctag catttgaaga acgccttaat atcatttatc cattgttgtg
     6001 tcaggtccct gggtttgagg ttgttaaacc acaaggtgct ttttatcttt tcccaaatgt
     6061 tacaaaagca atggaaatga aaggatatac ggatgttaca gcattcacgg atgccattct
     6121 tgaagaagtt ggacttgctt tagtaacggg tgctggtttt ggagcgccgg aaaatgttcg
     6181 tctgagttat gctacagatc ttggaaccct taaagaagct gttcgtagac tgcatgtctt
     6241 tatgggaagt aatgaaatta attaaataaa aatagagaga aacttatgtc aaaaaaactt
     6301 atttcaattg ttgatgttaa agattatgtt ggtcaagaag ttactattgg tgcttgggtt
     6361 gctaacaaat caggtaaagg taagattgcg tttgtacagt tgcgcgatgg ttctgccttt
     6421 ttccaaggtg tcgcttttaa accaaacttc attgaaaaat atggtgaaga atcaggtctt
     6481 gaaaaatttg acgttattaa acgtctcaac caagaaacat ctgtttatgt aacagggatt
     6541 gttaaagagg atgaacgttc aaaatttggc tatgagcttg atattactga ccttgaagtc
     6601 attggagagt cacatgaata tcctataact cctaaagagc atgggacaga cttcttgatg
     6661 gacaaccgtc atctttggtt gcgttctcgt aaacaaatgg ctgttatgca aattcgtaac
     6721 gctattattt attcaactta tgaattcttt gaccaaaatg gttttatcaa atttgatagc
     6781 ccaattttat cagaaaatgc agcagaagat tcaacagagt tattcgaaac agactatttt
     6841 gggaaaccag catttcttag ccaatctggt caattatacc ttgaagctgg agcaatggct
     6901 ctaggccgag ttttcgactt tggtccagtc tttcgtgctg aaaaatctaa aacacgtcgc
     6961 catttaacag aattttggat gatggatgcc gagtattctt tcttgtcaca tgaggaatca
     7021 cttgaccttc aagaagctta tgttaaagca ttgatccaag gcgttctaga tcgtgcgcct
     7081 caagcacttg atattttaga acgtgatgtt gaggctctca aacgttatat cgcagagcca
     7141 ttcaagcgtg tttcttatga tgatgctatt acacttcttc aagagcatga agcagatgaa
     7201 gatacagatt atgaacattt agagcatggt gatgactttg gttcaccaca tgaaacttgg
     7261 atttctaatt actttggagt gccgacattt gtagtgaatt acccagcaag ctttaaagca
     7321 ttttatatga aaccagttcc tggaaatcca gaacgcgttc tttgtgctga tcttttagct
     7381 ccagaaggtt atggagagat cattggcggc tcaatgcgtg aagacaacta cgatgctctt
     7441 gtggctaaaa tggatgaact aggtatggat aaatcagaat acgactttta ccttgacctt
     7501 cgtaaatacg gctctgttcc tcatggcggt tttggtatag gtattgaacg tatggtaact
     7561 tttgttgctg gtaccaaaca tattcgcgaa gctattccat tcccacgtat gttacatcgt
     7621 atcaaaccat aataaaaaag cgctagctag tgcgcttttt tattatggtc tttagaatcg
     7681 ttggttaatt aaattgggac tatgtttttt taacaatgtg attgtcgaaa agacaaggat
     7741 aagactgatt agtcgatcaa tataatctgt tccaacttga acaagtatca aactacttgt
     7801 catgtctaat ccaattccat gtaagaattg tgctataata cttgagccac tagaggtaat
     7861 tcctttaaaa aggataactg ttataactga agcaataata gttcctggta aagaaataat
     7921 cagtgatttc cacaataggc tagagggttt acaattcctt ttaatcaaga taccagtaat
     7981 gatagcaact acgatggcga ctggtgaata ataaagtgaa aaaatatcag tagtcatcca
     8041 attgatgagt gcactgagaa aagcagtgct ggcagcgtac catggtccca ggataactgc
     8101 tattagcaat gtacctatag tatctagata gataggtagg cgtaaaaata gagctaaatt
     8161 agccccaata aggttgatgg cgatagcgat tgagataaat gaaattaaac gagcaggtga
     8221 ttttgtcatt tgttaagtcc taacatttct aagtcttcaa tgatgactga attttgtgca
     8281 tgtaatagga cggtcataaa atcattccaa aataaattag tattgactga ggttaaaatc
     8341 ttactgttag catctttttt ataaaaatga tactgatcta caattgtttg tcccatggcg
     8401 attccgtgac aagcaacatc ggtatagcta tcaaaaccag tagcgatatt ttcattaaca
     8461 aagtaagcaa tagctaaagg atcatttatc acacatccaa taatatgctc atattgccag
     8521 tgaaaatcga agtagaactt tgtgattttt tggataaaag ttgatacgtc tggattaata
     8581 cgctccatat aagataaatg attgggggtt aatacaatat gacgtgtgat gtctagtcca
     8641 accatctcta tttttttgtc aaggttttca aatacatatt gtgcagcatg gggatcatac
     8701 caatagttgt attcagcaac tggagaacaa ttgccatgtg atttaaaatt tcctcccatg
     8761 ctaacaaatc gcttgcaatg tttgcctaat ttgggattag tttgtaaggc tcgagcgata
     8821 ttggtcagag gtccgagagc tataatagaa gtatcatttt tgtgttcaaa atagtttgct
     8881 aaaaaacaat ctgctgattc ttcttgaaag ataattggtt gcgccaatgt aaagttattc
     8941 tctccaaggc catccattcc atgagtatct tgagcagata caaattcacg ttgtaaaggc
     9001 aagttattac caacataaac tgaaatgtcg cgacgattta gtaattcaag cgttacaaag
     9061 gtattcttta gtcctagctc aacaggcgaa ttgcctgctg ttatagtaat agcaacaact
     9121 tctaacttag gatgctgaat agcatacatc agtgctaagg tatcatcaat accgggatcg
     9181 caatcgataa taattttttc tttgttcatt tgtctccttt cttttaatat tagtgaatgt
     9241 tctatgcttt tatgaaacta ttaacaatag tcatggcaat aaaaaagatc ttggcagtta
     9301 ccaagatcac acgaaaaaat gaatttagtt ttctaaattc aatcatattt tcagtcactt
     9361 agttttttat tctgggaagt tttcgaacca gtccgagaaa tcctcgatta tgcacgtgtt
     9421 ttattataat atgaaagcac taacttgtaa agattcaaac tgaccattta acagtgtcta
     9481 gattgattgt tttagcattt gaaattaatt ttgaaatcgg acatctgctg tgtgctttgt
     9541 taacgattag cttactatca gataatgaca gtttttcaat actagcaaca gcgttaactt
     9601 ggaaataaaa tcccttacct acttgctttt ccttcatgag ctggcaatta acatcaacac
     9661 gagattttag gtctttgaat cctttttgtt ctaaaatagc ttttatagtt gcatttaaac
     9721 atgttgacca tgctaaagca attaattctt caggattaaa tcctggtaaa tgatttaaag
     9781 ggtgattggt ctccacaatt tttccattgg agagataaac ttttccatca acgccttgag
     9841 tgtttgatga ggttacttca taaagcataa taaactcctt tgggtctagt ttaccaaatt
     9901 tgggagcatt ccgacaagat tttactattt agaatgagtt tcttttcctc tgaagtaaaa
     9961 tcctaattgc tagccatttg tggtataatg gcataaaaca taagagaaag aggtaatcgt
    10021 aatgtcagac gaacaaatca aacttgtgat tgtaacaggt atgagtgggg caggaaagac
    10081 agtggctatt cagtcatttg aagatttagg atattttaca atagataata tgccgcctac
    10141 tctagtgcct aagtttttag aattagcagc acaatcagga gatacaagca agattgctat
    10201 ggtagttgac atgcgtagtc gtcttttctt tagagaaatt aactctattt tggatagcct
    10261 tgaaattaat gataatatta actttaagat tctattttta gatgctactg acaccgagct
    10321 agtgtcacga tataaagaga cacgtcgtag ccaccctctt gctgccgacg gcagagtgtt
    10381 agatggtatt tctctagaga gagaactatt agcaccgctt aaaagtatga gtcagaacgt
    10441 tgtggacact agtgaattaa caccaagaca actacgtaag gtcatttctg aagaattctc
    10501 aaatcaagat agtcaaagtt cttttcgtat tgaagttatg agttttgggt ttaaatatgg
    10561 tatacctttg gatgcggatt tagtattcga tgtgcgtttt ttaccaaatc catactataa
    10621 accagaatta agagataaga caggtttaga tactgaggtt tatgactatg tcatgagttt
    10681 tgatgaatca gacgattttt atgaccatct tcttgcctta ataaagccaa tcttaccagg
    10741 ctatcaaaaa gaagggaaat ctgttttgac tgtggcaatc ggatgtacag gtgggcaaca
    10801 tcgtagcacc gcttttgctc atagattatc agaagattta aaagcagatt ggacagtgaa
    10861 tgaaagccat cgtgataaaa ataagcgtaa ggagacggtg aatcgttcat gagaaaacct
    10921 aaaataaccg ttattggcgg aggaacggga attccagtta ttttgaaaag tcttcgctta
    10981 gaagatgttg agattacagc ggttgtcacg gtggcagatg atggtggttc gtcaggtgag
    11041 ttgcgctcag ttatgcaact aacaccgcca ggagatttaa gaaatgtttt ggttgccttg
    11101 agtgatatgc cgaagtttta cgaacaaatt ttccaatatc gttttgcaga aggtgatggg
    11161 gattttgcag gtcatccttt aggaaatctt attattgctg gtgttgctga aatgcaaggt
    11221 tcaacttata acgctatgca gagtttaaca cagttctttc atacaacagg aaagatatat
    11281 ccatctagtg aacatccgct cactctgcat gctgttttta aagatggcca tgaagtggtt
    11341 ggagagagtc aaatagccga ttataaaggc atgattgacc atgtgtatgt cactaatact
    11401 tataatgaag agactcctac ggccagccgc aaagttgttg atgcaatttt agaaagtgat
    11461 atgattgtgt tgggtccagg gtcacttttc acctctattt tacctaacct agttattcca
    11521 gagattaaac aagctctttt agaaactcgg gcagaggtag cttatgtatg caatattatg
    11581 acacaacgtg gtgaaactga gcattttaca gacgcagacc acgttgaagt attgaaacgt
    11641 catttaggac aagatgctat cgatacagtt ttggttaata ttgaaaaagt gccagagtct
    11701 tatatggaaa acaatcattt tgatgagtat ttggtacagg ttgaacatga tttttcaggg
    11761 cttagaaaac atgccagacg tgtcatctcg tctaactttt taaaattaga aaaaggtgga
    11821 gccttccatc atggtgactt cgttgttgag gaactaatga atttagtagg aacacattta
    11881 tgagttttac agtaaaagtc aaagaagagt tgctaggtca taagagtgaa aataagatgg
    11941 aactgtcagc tattatcaaa atgtcaggta gtttaggatt agctaaccac ggcctcaacc
    12001 tttctattac cacagaaaat gctaaaattg ctcgtcatat ttatagcatg ttagaagaac
    12061 attatcattt gcagccagaa atcaaatacc atcaaaaaac gaacttacga aagaatcgtg
    12121 tgtatactgt ttttattgaa gaaaaagttg atgttattct tgctgatttg aagttggctg
    12181 atgctttttt tggtattgaa actggtattg aacactctat attggacaat gatgaaaatg
    12241 gaagagctta tctcagggga gcattcttat caacgggtac tgttagagaa cctgattctg
    12301 gaaaatatca actagaaatt ttttctgttt atctagacca tgcacaagac ttagctaatc
    12361 ttatgaaaaa atttatgtta gatgcaaagg ttattgaaca taaacatgga gcagtaactt
    12421 atttgcaaaa agcagaagat attatggatt tcttgatagt cattgatgcc atggaagcta
    12481 gagatgcttt tgaagagatt aaaatgatac gtgagacacg taatgatatt aaccgtgcta
    12541 acaacgttga aacagctaat attgctagaa caatcacagc tagtatgaag actatcaata
    12601 atattatcaa gattatggat actattggtt ttgatgcact gcctagtgat ttaagacaag
    12661 tagctcaggt gcgtgtggct catccagatt actctattca acaaatagca gacagtttag
    12721 aaacgcctct gagtaagagt ggcgttaacc atcgcttacg gaaaataaat aaaattgcag
    12781 atgaattata gaaggagata ttagcatggc ttgtacaaca atattggttg gtaaaaaggc
    12841 ttcttatgat ggttcgacta tgatcgctag aacggaagac tctgttaatg gcgatttcac
    12901 acccaaaaaa ttaaaggtaa tgacatctaa agatcaaccg cgtcattaca aatcagtttt
    12961 atcaaatttt gaagtagatt taccagataa cccacttcct tatacttcag taccggacgc
    13021 attgggaaaa gatggtatat ggggtgaagc cggtattaac agtaaaaatg tagcgatgag
    13081 tgctacagaa actattacaa cgaattcccg cgttttgggt gcagatcctt tggtttcaga
    13141 tggtataggg gaagaggata tactcacttt agtgcttccc tatattcagt cagcgcgaga
    13201 aggtgtggag cgtttaggtg ctattttgga aaaatatgga acctatgaat caaatggtat
    13261 tgctttttca gataccgaag aaatatggtg gttagaaaca attggtgggc atcattggat
    13321 tgctcgtcgc gtacctgatg atgtttatgt tactaatcct aaccaactag gaattgatca
    13381 ttttgaattt aataactgtg atgactacat gtgctctagt gatttgaaag agtttatcga
    13441 acaataccat ttagatttga cctattctaa tgagcatttc aatcctcgat atgcttttgg
    13501 tagccaacgt gataaagatc gtcattacaa cacaccaaga agttgggcaa tgcagcgttt
    13561 tttaaatcct gaaattgaac aggatccacg tagtttgttt attccctggt gtcaaaagcc
    13621 ttaccgaaaa attactgttg aggatattaa atatgtgttg agtgatcatt atcaagacag
    13681 tgtgtatgac ccatatggac cagaagggga tgcggtaagt aggagagctt ttcgttcagt
    13741 tggtatcaac cgaactagtc aaacgtctat tctacaattg cgaccaaata aatcacttga
    13801 aacgacaggt gttcaatggt tatcttatgg ctctatgcca tttgcaacca tggtgccgtt
    13861 gtttacacaa gttgagactg taccagacta tttttcgaat acaaccaagg atgcttcaac
    13921 agataatttt tattggacca atcgtttaat tgcagctcta gcagatccac acttttatca
    13981 acatgaagct gatattgaaa gctatatcga gagaacgatg gcccaaggac atgcacatat
    14041 taatggtatt gatagagaag ttgctgagaa taaagagatt gattttcaac agaaaaatca
    14101 agaaatgagt gactatattc aaaaagaaag ccaagaattg ttaaatcgta ttctatttga
    14161 tgcaagtaat ttaatgacaa atcgcttttc aatgggagat taaaaaactc ggaatatttt
    14221 ccgggttttt taagataaaa acgcttgcat aagtatataa aacttgcaaa aaaaaaatat
    14281 cgtgctatac tttactagta aaatattaac cggtaaataa cgggttaaat aaaaaggaga
    14341 aaaaatgaga aagaaatttc ttttattgat aagctttgta gctatgtttg cagcttggca
    14401 acttgttcaa gttaaacaag tttgggctga tagtaaactt aaagtggtaa caacttttta
    14461 cccagtttat gagtttacaa aaaatgtcgt tggtgataaa gctgatgtat ctatgttaat
    14521 taaagcaggt acagagccgc atgattttga accatcaact aaaaacatcg ctgccatcca
    14581 agattcaaat gcttttgttt acatggatga taacatggaa acttgggctc caaaagtagc
    14641 taagtcagtt aaatccaaaa aagtaacaac tattaaaggt actggcgata tgttacttac
    14701 taaaggcgtc gaagaagaag gtgaagaaca tgaaggacat ggtcatgaag ggcatcatca
    14761 tgaacttgac ccacacgtat ggttgtctcc agaacgtgcg atttctgttg tagaaaacat
    14821 ccgtaataaa tttgtcaaag cttatccaaa agatgcagct tcatttaata aaaatgcaga
    14881 tgcttacatt gcaaaattaa aagagcttga caaagaatac aaaaatggtt tgtcaaatgc
    14941 taaacaaaag agttttgtga ctcaacacgc agcgtttggt tacatggcgc ttgattacgg
    15001 tttaaatcaa gttccaattg ctggtcttac tccagatgca gaaccttcat caaaacgttt
    15061 aggcgaatta gctaaataca tcaagaaata taacatcaac tacatttatt ttgaagaaaa
    15121 tgcttcaaat aaagttgcta aaactttagc agatgaagtt ggcgtgaaaa cagctgtgct
    15181 tagtccactt gaaggacttt ctaaaaaaga aatggcagct ggcgaagatt acttctcagt
    15241 tatgagacgt aatttgaaag ttcttaaaaa gacaacagat gttgcaggta aagaagtagc
    15301 tcctgaagaa gataaaacta aaacagttga aacaggttac tttaaaacta aagatgttaa
    15361 agaccgtaaa ttgacagatt actctggtaa ttggcaatca gtatatcctc ttcttcaaga
    15421 tggaacactt gatccagttt gggattacaa agctaaatct aaaaaagata tgactgctgc
    15481 agagtacaaa aaatattata cagcaggtta caagactgac gtagaatcaa tcaagattga
    15541 tggtaaaaaa caccaaatga cctttgtacg taatggtaaa tcacaaacat ttacatacaa
    15601 atatgcaggt tacaaaatct taacttataa aaaaggtaat cgtggagtac gttatctctt
    15661 tgaagctaag gaaaaagatg ctggtcaatt caaatatatc caatttagtg accatggtat
    15721 taaaccgaat aaagctgaac acttccatat cttctggggt tcagaaagcc aagaaaaatt
    15781 atttgaggaa atggaaaact ggccaacata cttcccagct aaaatgtctg gacgtgaagt
    15841 tgcccaagac cttatgtctc attaatattt aaaagatgat atcggatacg atatcatctt
    15901 tttttcttcg tacgtgatag gctcaaacat agaaaaaagc acttctatta gaagtgcttt
    15961 taaaattaat gctatgctta ttgagcagcg tttgcgtctt tgagaccata ttttttgttg
    16021 aaacggtcca cacgtccatc tgcttgagtg aatttttgac gtcctgtata gaatgggtgt
    16081 gaatctgatg agatttccac acggataagt gggtaagttt caccttcgaa ttctactgtt
    16141 tctttagttg atttagttga accactaagg aatttgtaac ctgtagttgt atctaagaaa
    16201 acaactggac gataatctgg atggatgtct tttttcatct ttaaaaattt cctttctgcc
    16261 ataactcgtc tgagtcatag ttattaacca ctctattcta tcaaataaga atagccttga
    16321 caagtgtttt gataaaaaaa gtaaaattat aaagaaagta cttcctggat ttcttggtaa
    16381 atagcttgat tctcttccaa actataagaa ttagctccac tggctaaagg atggccgcct
    16441 ccattatgac gtttagctat ttcattaatg atgtgtgact tactacgtag gcgcacacga
    16501 taatgcccat cgctttgttc aacgaaaatt gcccaacttt ctacaatatc aatttttcca
    16561 ggtataccaa cgattgcagc agtctctgca tctgttacat caaagcgttt taagtcttct
    16621 tgtgttaaag taacgcatgc agcgccgttc ttgtcaattt caagttgctc aaaaataaag
    16681 ccttgtaatt ttgcaatttt gaaaggaaag gaatccattt gacgtgccat tgcagaaaaa
    16741 tcaaagtcaa attcacgaag tttgctagca atcttcagtg ttttacttgt ggtagcagga
    16801 tataaaaagc gtcctgtatc tcctacaata ccattatata gaactcttgc tgcacttgtt
    16861 gataacaaca aatcacaaga aagagcaaag tctgtcacaa tttcacttgc actagaggcg
    16921 ttagtatcta cataagagag gtctccatag acttcatcgt ttggatgatg gtcaatttta
    16981 attaaaaagt cacctttttt ataacgttca tcatcaatgc gaggggtatt tgctgtatct
    17041 gtaacaataa caagagcgcc ttgatagtct tggtcggtca cttgatccat tttagcaatc
    17101 caagcgagtg tgggttcgtc aaaaccagta gcaagtactt ttttctttgg gaaattatgc
    17161 ctgatgatat cccttaagcc gatttggcta ccaagagcat cagggtctgg tctcatatgt
    17221 cgatggataa tgatggtgtc gtactctttg attttatcaa gaatttgttg aaaaataatc
    17281 attttttacc tacctaactg ttttgtcttt tagtgaagaa caaggctata ttgttgacta
    17341 tattatagct taaatctttt attgaggaaa gaaattaaga tagcggccgc aatgtttgtt
    17401 tatttttata tgatggtatg ttattttttt ataaaattcg gatactgcta actaacaatc
    17461 ttttttatga taatattttg taaatgtatt gctttttagc taacttttgt taatatagta
    17521 aaaatgcttt ttaataggag ataaattatg acacaagcag tattaaaaga attggctaag
    17581 gcggagttgc attgtcatct agatggctca ctttctttac cagctattag aaaattggct
    17641 aatatggctg atataatact gccaaatagt gataaagaat taagaaaata tgttatagct
    17701 ccagctcaaa cagaaagctt ggtagattac cttaaaacgt ttgagtttat cagaccgctt
    17761 cttcaaacga aggaagcatt acgttttgct gcttatgatg ttgctagaca ggcagcttta
    17821 gaaaatgtta tttatattga aattcgtttt gcgccagaac tctctatgga taaaggttta
    17881 accgcaagtg atacagtctt tgcagtctta gaaggtttag cagatgctca gaaagaattt
    17941 aatatcgttg cacgcgccct tgtctgcggt atgcgccaat cgtctcataa aacgaccaag
    18001 gaaattatta aacacattgt tgatttggct cccaaaggcc tggtaggttt tgattttgca
    18061 ggtgatgaat tttcctatcc aacagatagt ttagtagatc ttattcagga agtaaaacgc
    18121 tcaggttacc cgatgacttt acatgcaggt gagtgtggtt gtgctaagca tattgcggat
    18181 agtttaaatt tagggattaa gcgtatggga catgtgacag ctttaacagg acagagggct
    18241 ttaataaagc gatttgtcga agaagatgct gtagcagaga tgtgtttaac tagcaattta
    18301 caaacgaaag cagcctcaag tattcaaagt tttccttatc aagaacttta tgatgctgga
    18361 ggtaagatta caattaatac agataatcgt actgtttccg atacaaattt gacgaaagaa
    18421 tatagtcttt tcgtaactta ttttggaacg aaaatagaag actttcttgt ttttaatcaa
    18481 aacgctgtga aggcctcctt tacaagtgat tcagagaaag atactttact acataagcta
    18541 caagaaaact atgacagcta tctaaaataa ccttcacgat tctttaaaaa tatgatagaa
    18601 tagtggtaat ttacaagtgg aggaagatat ggctttagca aagattgttt atgctagcat
    18661 gaccggaaat actgaagaaa ttgctgacat tgttgctgat aaattacgtg acttgggtct
    18721 agatgttgaa gtagaagagt gtacgatggt tgatgctgca gattttgaag atgctgacat
    18781 tgcaattgtt gccacttata catatggtga tggagattta ccagatgaaa tcgtggactt
    18841 ttatgaagat ttagcagaag ttgatttatc tggtaaagtt tacggcgtag tgggttcagg
    18901 tgatactttc tatgattact tctgcaaatc tgttgatgag ttcgaggctc aattcgcatt
    18961 aacaggagca caaaaaggtg ctgattgtgt taaagttgat ttagcggcag aagacgaaga
    19021 cattgaaaac ttagaagctt ttgctgaaga aattgcttct aaacttaact aagctaccaa
    19081 aaagacgaac acgagtcgtc tttttaactt ttttaccatc ttttgtaact agaatatgag
    19141 atatagatat ggaattagaa gagattcgtc aagaaataga tgaaatagat cagcaattgg
    19201 taagcctttt agaaactaga atgggtctaa ttttagaggt catagcattt aaaaagaaac
    19261 accgacttcc agttttagat aataatcgtg aaaacgaagt gcttaacaat gtattgaaga
    19321 aagtccaaaa tcatcaattt gacgatgtga tacgagcaac tttcaaagac attatgacag
    19381 aatctcgtgt ttatcaaaag gagaatattg tagacggtga ttaaacgatt tgataattat
    19441 gctcagtacg tacttgtttt agggtttagc ttactaatcg gtggtgtggt tggggctatt
    19501 acagcagtct ttggacgtgt tctattattc ctgacagcct ttagaagtga ttatatagct
    19561 tacctactcc cttttcttag cattgttggt ttatttatag tttttgtcta tcaaaaattt
    19621 gggggtaaga gtgttaaagg gatgggattg gtatttgaag taggtcatgg taatgaggag
    19681 actattccga aacgtcttgt tcctctcgtg atattaacaa cttggttaac ccacttattt
    19741 ggtggtagtg caggtcgtga gggggttgct gtacagattg gggctaccgt ttcacactac
    19801 tttcaaaaat attgccgttt acaaaacgct tctcaacttt tcctagtaat gggaatggca
    19861 gcaggttttg ctggtttatt tcagactcct ttagctgcta ctttttttgc gatcgaagtt
    19921 ttggtagtag gtcgtttaat ggtttcgtat atattaccaa gtttaattgc tgctctgaca
    19981 gcaaatttcg tctcacattc tttaggttta gaaaaatttt ctcatagtat agcaacatca
    20041 atggcgttaa caccagatat tattctaaaa ttattggttt taggactatg ttttggtctg
    20101 tgtggcaatt tatttgctta tttattggct aaagctaaat taattgcttc tagtaggtta
    20161 ctaaacccat ataaacggat tttcacactt ggattgttag ctactttttt attgtttatc
    20221 ttccattttg gacgatattc aggactggga acaaatctaa ttgaggctag cttcactaat
    20281 aagaatcttt atgattatga ttggttgtta aaattgtgtt tgacagttat tactttggct
    20341 gcaggttatc agggaggaga agtaactccc ttatttgcaa taggagcaag tttaggagtg
    20401 attattgcgc ccattttagg tttgccagtt attttagttg ctgctttagg ctatactagc
    20461 gtttttggga gtgcaacgaa tactctgtta ggtcctatct taataggtgg cgaagtcttt
    20521 ggctttgcca acactcctta ttttgttatt gtatgtcttg ttgcttactc catatcacat
    20581 gctcatacga tttatggtgc tcaatctaga tagttagtga tgagttttaa aagagtttat
    20641 cagtttatag aacggaatat tccgttctct tttttattct cttaaagttt ataaaaatga
    20701 agtttgtaaa ttttatgaat gcaaaaatta tgtggctaat ataattttct tcaaagaaat
    20761 tgattttatg attgtcatta aaatactgta aaatccaatt gaatgtgtaa gaacttaggt
    20821 taggataatg tctacaagca tttaagtcgc gatggtgaaa tgcattagaa gataaaaagt
    20881 tgatgtggat ttcaacttta tgggaaagag tcaggtgaca cgaaagctaa tatagaaaac
    20941 gtagtttaaa aaatcatata atattaaatg aattaaactc tcaaaaatca gaaaaaagct
    21001 aacctagaaa cagtaaatca aatttcaata atatacaaat aactattcta aaggatgaca
    21061 ccttgttctt ctagataaaa tagctttaca agcaggttaa aatttggtag aattgtgaga
    21121 gtgtgtaatg gacgcacaaa aaacggctaa tccgctgaga caagtactta agattagtaa
    21181 gagaaggaga ataaaaatga atccattaat tcaaagtttg acagaaggtc aacttcgttc
    21241 tgatatccct gagttccgtg ctggtgatac tgtacgtgtt cacgctaaag ttgttgaagg
    21301 tactcgcgaa cgtattcaga tctttgaagg tgttgttatc tcacgtaaag gtcaaggaat
    21361 ctcagaaatg tacacagtac gtaaaatttc tggtggtatc ggtgtagagc gtacattccc
    21421 aattcacact cctcgtgttg ataaaatcga agttgttcgt tatggtaaag tacgtcgtgc
    21481 taaactttac tacttacgcg cattgcaagg taaagctgca cgtattaaag aaatccgtcg
    21541 ttaattttga tgatcagatt ttaaaaatgc ttggttgttt gaggatagta actatgtttt
    21601 aaaactggac aaccaagacg taaaaaatct gcctgtgggc agttttttta ctaggtcccc
    21661 ttagttcaat ggatataaca actccctcct aaggagtaat tgctggttcg attccggcag
    21721 gggacatatt cattgcatgt aaatagcggt ttagagctat tttgccccaa atccgcccca
    21781 aatttctctg attaagttta tcgttcctat ctttttgttc ttgtaattga tgtgcgtaaa
    21841 cttctaaagt gatatttaaa ttctcgtgat ctaaaacttg agagatggaa attagatagc
    21901 ttgcaaatgt atgcctgaga gagtgcactc gtacctcgcg accagttatt tttcggatag
    21961 ttttattgac tgcattattt gaaagtttgt cgaataatct gtcgttttta ttttttgtaa
    22021 attcatgcaa aaaaaataat gtatcattgt caattggtat atttctgata ctacttttgt
    22081 tttttgttgg caggtatctt tggttgaaat gataatccca agttttatta attgataaat
    22141 atttgttagt gtaatcaata tcattaactg ttaaacctaa acattcagcg aagcgcatgc
    22201 cagttttagc gatgaggtat aacgctgcat acgattgatg ttgtgatttt tctttacaaa
    22261 tttttatcaa gcgtaagtat tcattggttt caagaaattt tatctttatt tacgcccctt
    22321 attttttgct ttaaccttag tgaataaaca aaaatttttt tctatatatc cctcgtgaac
    22381 agccatggat acgcaggctt ttacatgtat gttaaaacgc tttactgtat cttgcacatg
    22441 cgtttgacta taatgattta tgacttgttg atatttagtg gaagtaatat tgcaaagtaa
    22501 tatatttcct attatatgtt tatacgatat tcgatattcc cacccgttgt cgcgtttacg
    22561 gaaatacgcc attgatatac tccacattag ctaaagaaca gggtgttcaa ggctaccttg
    22621 atggaaaagg ctctcttaga gatatttgta aatggtatga tatctcaagt cgctctgttc
    22681 tccaaaagtg gataaaacgg tatactagtg gtgaagactt gaaagccact agtagaggat
    22741 atagccgtat gaaacaagga aggcaagcca catttgaaga acgtgtagag attgttaact
    22801 acaccattgc ccatgggaaa gactatcaag cagctattga gaagtttggt gtttcctacc
    22861 aacaaattta ttcttgggtg cgtaagcttg agaagaatgg ctcacaaggt ttggttgata
    22921 gacgtgtgaa agggttggag agtaggcctg atttaaccga gattgagcaa ctttaactca
    22981 agattaaaca attggaggaa cgtaatcgtc tcttagaaat cgaggttagt ttactaaaaa
    23041 agttagaaga catcaaacga ggaaacagac ggtaagacta ggtaagcatt tagcggagtt
    23101 ccaagtaatc aagaattatt acgatgagga atctaatgtg cctattcagg ccttatgcca
    23161 actcttgaag gggtctcgtt caggctatta caagtggctc aatcgtcaaa aaacagattt
    23221 tgagacaaaa aatacaaagc taatggctaa aatcaaggaa cttcatagac tctacaatgg
    23281 tatcttaggt tatcgccgta tgacaacatt tattaatcgt caacttggga caacttaaaa
    23341 caagaaacgg attcgttgat tgatgaacat tctggggatt agttcagtca ttcgtcgtgt
    23401 tagccatgct tgtacaaaag ctggtgacag attttacgaa gaaaatattc ttaatcgtga
    23461 atttacagcc acagctcata accagaaatg gtgcacagat gtcacctatc ttcaatacgg
    23521 tctgggagct aaagcttatc tcagtgcgat taaagacctg tataacggtt ctattatcgc
    23581 ttatgagatt agtcacaaca atgaaatcca cttgttatga agaccattaa aaaggggcta
    23641 gagctcaatc caggagccac acctatcatc catagcgatt gaggtagtca atatacttcc
    23701 aaagaatacc gttatatcat acaacaagct ggtctgacct tatccatgtc ccggattggc
    23761 aaatgtattg ataatgcacc aactgaaagt ttctttgggt ttttcaagac tgagtcttac
    23821 caccttaaga aatacaactc ttatgatgag ttggtcaatg atgtggcacg ttatatcgaa
    23881 ttctacaaca cacaacgtta tcaatcaaaa ttaaacaatc tgactcctct agaattcagg
    23941 aatcaggttg cataacttat cttttattat ttgactgtct acttgacagg gagccgttca
    24001 gattgcttaa cctttctaaa tttgctaaaa tagctacaag aaaacgagcc atttaatgct
    24061 tatttcttat actgtcttgc ctcacgctct cctcgaccaa aaattgagcg tgaggctttt
    24121 tgtttcatta aacgatgata tttccatatt catcagtttg ttttccgaga gccatcaaag
    24181 cttcgataag gtcgataatt ccaggaataa aggtaatact aaaaataata tataaaaaaa
    24241 cctggcctat ttttcctgcg taaaatttat gcgctccaat gccgcccaaa agaacgttaa
    24301 taaaacataa actactatgt tagcataaga ct
//
LOCUS       DATRUK010000027        24015 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.027, whole genome shotgun sequence.
ACCESSION   DATRUK010000027 DATRUK010000000
VERSION     DATRUK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24015
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.027"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            419..682
                     /locus_tag="VBE34_001045"
     CDS             419..682
                     /locus_tag="VBE34_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263772.1"
                     /translation="MFGFPDVLTMTHKQKMQIGQYLAEGYVTSTEVLNMIERIPKDSA
                     SPLAYLLKSLENLKQERQFEQKRMAHLNAENYYSMKKEGIENG"
     gene            675..839
                     /locus_tag="VBE34_001046"
     CDS             675..839
                     /locus_tag="VBE34_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263773.1"
                     /translation="MVNLILVLIAAVIVFIFVFLAVEIHDTPLMADDEMTVISSPKHD
                     HDEHHSQKAA"
     gene            1073..1399
                     /locus_tag="VBE34_001047"
     CDS             1073..1399
                     /locus_tag="VBE34_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263774.1"
                     /translation="MSNLLQVSDKPFLTLSDLRLLGRQGEAMAYLDDGRQVIIKPKFA
                     VVQKKRTINGKITIVGEDILRFHEIYSAIKFIKRKHFIIAERVKRNGKYVLVLTGRGY
                     HRQRKE"
     gene            1410..2159
                     /locus_tag="VBE34_001048"
     CDS             1410..2159
                     /locus_tag="VBE34_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7263775.1"
                     /translation="MKKVKALALFSTAILATASTGVYADEVISDGTTSPSTEQVDPTP
                     APSSSEVTPGSESNIPTPSSSSSTEAPEGSKRGSETTETEPTQPATEPEKSTETPSEQ
                     PGEVEVPTTDGGKATVVPDTSVPTNNPNITAETAQNAGASQVGTTSTVTGQVVRDVTR
                     SNPVTLYNGASLVDIRDGLVTLSTGEKVAPEVVGVKSNADGTYTAKTIQGDTVTLPHT
                     GEKNSTVLSVVGALILSVLGFGLKKRKNQEI"
     gene            2220..4877
                     /locus_tag="VBE34_001049"
                     /pseudo
     CDS             2220..4877
                     /locus_tag="VBE34_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEC10/PgrA surface exclusion domain-containing
                     protein"
     gene            5601..10124
                     /locus_tag="VBE34_001050"
     CDS             5601..10124
                     /locus_tag="VBE34_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7263776.1"
                     /translation="MSNKFIQQSQIKKGHGFFRKKKWAKGLASGIALGAVVTFSSSAY
                     ADDIANTVDTQPEVTIVSDSNATNLADAQADNSQEHTGLTNQSGEQTGQMTDKITSDG
                     LDQSVSDAQTAGVEVDQNGTVTHSSYADAQADLAKQKEAIDQAVETQKEVDNAKATAE
                     EAAKAAGVEVSTEASKSAKTYTSVDDAKVDAKKQVDNLSTVTEAQKQVNEQLPKAVEA
                     ATGSGVKVSVKAGAKTTDAKKALENLANQVANLKKAQDTQNTISNSMAQALEEAKKAG
                     VTVSTTGSKSYDDLSKALADAQAQVAKLTEFKETQASIDEAIKKSADNAKASGVTVST
                     GDAKTYTSAADAQADSDKQTATLDKAAETQKVADKVISDLTKEAQNAGLDLTKGTSQT
                     YTSVEEAKKDIAKQERALKDATTAKANAETTNSSNKKAAADAQAAANKAINDAIAKAT
                     ASGTTVTKTSTIETTVEEAQKIAKEQSDKVNDVAAKNEQIQNENTAKKNAYDAKKAEV
                     DKKNKAAFDRAGLNYTGDYATDKATADKFNSDEIERVKSQFEKANDNNVLGATDGWVQ
                     HVGSNGYTQSGTSQFKKVQNVNGVEIVANGSLEPGKATVYVKGNVNQSHVVANMNWGN
                     VAPQGDNLTSNIGSKWAEAYDYNADGTTTQIWKGKILKWYRIPNAITMLDGSRHDAYV
                     LFHTDTSGLSSPGDEVVFWNQDGAINAVDGYRYGVNDKSDGIRTVIRVDSPDNNDNYL
                     WISLLGDFDIGQFLEADGVTVLGVGGGFATNSGVGGHRVAVDENLGLTYGKNTTPTSA
                     LTGFNSAPDGVALIAQYSNQYSTVIRNTAGGNGVAVARTDFGAQAKVSIVTPRHVNVE
                     NVETPTYTELLPVSVQDVHITPGQVTVPKAVTLHDVNVQAETHAIKLATEVAPVTVET
                     EVTTPALSATVHDITLKTAVHPVEVTQKPTNEKAVTNNDNVDINGKTVAKGSIVNWVL
                     KNGNLLAGREELTAVVMNDPFPAGFEVDSEATAKANADYYTLKTTENGYQLAGTAKLL
                     SLLNAHRDQDVEVPGFVFVGKPLNDNAHYDNAFETIYSTAKGYYKVVSNIPSIDTPDN
                     PKPTKTVTDKAGSDIDGKTVFDKDVTFHLITDYSTYVNTVVDVNTTTKAAGIYDDTDD
                     ATVKPDVTKATVTDSKGNDITAKGTFYDIEAGQPIPTEIQTFLDGANLTPNGEFIVWI
                     PNDLKDYYENYVLTGNNVTVNLPVEVIAQPGEKAENTFVQVEFGNGYQSNIVFVKVPD
                     VSPEKHAISKDGTVLDGQEVGLNQVFNYKLDGVTVPKDHDTLWQYDGKDKLDIEHDRY
                     TGNWKGVITGTEYTAKEDMTLTYDVITEDGTVIKAGDTIAAGTNYHFIFEFNQDTNDD
                     FINKIVKVTWDAQKGEWAYSIDEDFLKSLGVEGTFDADFWIEVERIKDGEVENTFINT
                     INGRELVAKVTTHTPTPETPAKPVEEVPAVKASVLPTTGEKTTALNVLASIVGLVMTV
                     GVAFGLRRKEKHN"
     gene            10342..10569
                     /locus_tag="VBE34_001051"
     CDS             10342..10569
                     /locus_tag="VBE34_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263777.1"
                     /translation="MDTAVRVVTALGAILSIIGLGWVLTGAFDYFAGRKNGNPAMMDQ
                     GMTAMVSGGAVAVISAGVAAAIVAAMHSISF"
     gene            10650..11207
                     /locus_tag="VBE34_001052"
     CDS             10650..11207
                     /locus_tag="VBE34_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263778.1"
                     /translation="MNKEFDYVAFAKNFEATNGRPPTADELDNAKWEHFGIRWHTPEE
                     SQAELERINASYKPTLGEHLKDALGFLGRGFFKALFFVLMSPLYLTMLFFNLIKSALG
                     VFLVWFVSKFMFLLVIGMIADLRGESEITGVSKSLVDCLFGSDFISTGNPNFFPHPVL
                     DAWIIGIVIVLLALVATFSKVEEHK"
     gene            11266..12015
                     /locus_tag="VBE34_001053"
     CDS             11266..12015
                     /locus_tag="VBE34_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263779.1"
                     /translation="MDKIVSELTETLGSYNKTVNSSIGAINKSLMAIGYILISILFLI
                     EMLSWYRFIRNQGGEMTWKLFLEVAVKYFIAYFLVAQSGAVLNAIMWFTNGITKLIGV
                     DFKGAGFQYVPIKHGKYGIRLMIDGIGFIVGAAATLSLKVIVLLRFIELYFFKAIAPV
                     IVAFWMSDNTRHIAVNFLKMFGAVALQGAVIILLLAIWQGFRVDTLVQLDKNSWLGAY
                     ASGFSYIGKCVVFLILLIGSQRKAKQLLNVS"
     gene            12132..12539
                     /locus_tag="VBE34_001054"
     CDS             12132..12539
                     /locus_tag="VBE34_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001877537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7263780.1"
                     /translation="MSEETGIAYPTLSGYNQGIRTPKKKNAQILADYFGVSVPYLLGL
                     DDNPPALNDSLEKIMVIHEVLNAGDNILVAQELLKNSLETLVKYADNDYRVQFKHHLL
                     ISNTLSEVIGKLDIVTENDELNFTPDKLNLSNN"
     gene            12646..13002
                     /locus_tag="VBE34_001055"
     CDS             12646..13002
                     /locus_tag="VBE34_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEO7263781.1"
                     /translation="MNKLGSEFFKAIDQFERSVVGGFTLRQLIMLIGIVIGVALATLI
                     SFLKLPDILFYLVLALTVPPSFIYGIKKDEQIKEVLMFKFTVQERAYQTDFESEDMNG
                     TFIQQKGVHEWNDTFD"
     gene            12977..15337
                     /locus_tag="VBE34_001056"
     CDS             12977..15337
                     /locus_tag="VBE34_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7263782.1"
                     /translation="MNGTTPLTKEDKERAKQLKKALKASTQNTIKYTSLFEDGLMHIV
                     DEEYSKTWELGDTNYLTADEDEKLDIIDYYVEALNGLDSDNTYQLLIINRPVPSTLLS
                     QITYELESDDRDVYRQEYNDMITSRFATDQNNFKVEKFITVSTSARDRKQAYRKLNDV
                     ENNFSKQFQVIDIPFTPLTGTERLNIFADLLRGNPYLNVDYKDVRISGLTTKSFVAPG
                     RIWFQHHQFMIDKKYCKVLFARSFPAFLNDRLIKSITDIGIELAITIHAKPYDPADAV
                     QKINTAEAGVKMDMIKSQKVAADKGISGNLAVSGVAQATAEEAAKWSTEINDNDQKMF
                     SGIFAVMLKADTPEELADYTNRVKQAGRKHVVDFEETYYHQEEALNTILPIGKTYLDV
                     KHRFMRDMTTTNIATQIPFTNTDLQSSSPMAIYYGQNQISNNIITLDRQRDLNTSSGV
                     ILGSSGSGKSVFVKTSEVIPTILRYPDDRIIIVDPEDEYSDIGRAFDAQLIDIFPGSQ
                     THLNLMDLPDEENLSAEDADPIGEKSSLIMGLFENILQEVTDEDFSIIDRVTRLCYER
                     ITDRTPTLKDWYDILVEQPEEVAQDLALKSESYTNGSQDIFAHETNVNLNNQVVIFNL
                     KKLDGKLKPFALMVVQDYIWNQVVNNQGKLTTRIYFDEMQNQFLTDNQAEFFTNLYAR
                     VRKYGAIPTGITQNVETLLNRTEGRKLISNSEFIVLLKQKKTDLAYLTQTINLTDALI
                     RYIEKPKAKGTGLIIAGQTAVPFENPIPKNTELFRLVATDAYRQID"
     gene            15381..18044
                     /locus_tag="VBE34_001057"
     CDS             15381..18044
                     /locus_tag="VBE34_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7263783.1"
                     /translation="MSNKDDLRKRVVRKRHIKESVQRHNQLSAKKAQNAAKKEHREAK
                     IKVKHARAALRRAEKGIDKDVDGEKIVDDLEKALAEKKATKKIKKKVRKTNPTIGQKA
                     RRTGKNIALNASHQIKSTAMDQDDTLSDLQNLQYRTRQSKAVAKQAGKVTWRGAKLAY
                     RKSKGMVNVSRGAYRSAKDSAKFATKTTAKVATKVAQTTASAVKTATSAAATILANPM
                     TWIVSAVVGFTLFLVILVSSIFSSNVIQQNDFTLNQSWLHISQVDRQKSSDQVEYYTD
                     IDSILLYMNFRYGGEWEPDANWDDGTGGKLTGALGFNHFSDALDDIWKEENSDIDNLK
                     TMAELYTNGKAWMKLDKDDLADYKEILESQSETGKYLATQELINPFYSADDAKSDTDY
                     LTISKRYGYTSKDKIDPTTTLQAQSGQKLYAVMDGKITVTTKDLKGNETKTTNLIISD
                     NDARFIFYNISAIRVKTGDKVEAGAELGTVSGGSQKIAYAKNYGEVDSKDSQWLQDIK
                     NKDVSYGYTEQTKKNKTNVWVLVNPGFYFPFVKYAQTTVVMRTSSELSGRAKQFYDLM
                     KKFYPSATDNGIAAVVGNFAVESSITPKRAEGDYLNPPIGASDGCWDDVNWLNMGGTA
                     IYGGGYPNILHRGLGLGQWTDTTDGGTRHTMLINYANSIGKKWYELETQIRFMLEGDS
                     PYYQNILKSVLTSNSDTDSLTRTFLTKWEGINNGTLSQRQSYAKQALSWFHQSIAGGG
                     TMASSWNFPEEYRDKIKSMPTGVSMTTQPGSGYPAGQCTWYAYNRLVELGEITDLSGS
                     YGHLGDGKSWVGNLVAKGWKFSTMPTAGAVCSTAGGFDSTYPQYGHVMIVEVVNADGS
                     FLVSECNYAGNKSQIHWRVCRNAPYYTFATP"
     gene            18061..18666
                     /locus_tag="VBE34_001058"
     CDS             18061..18666
                     /locus_tag="VBE34_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263784.1"
                     /translation="MIDEFKVTFFKRVMMIVGIIIIFLIGLGFGSMSKKTDNQSSSHT
                     TLTKKVKEGHQLTEGWVKQFLIAYYTKKDLEENRNRYKDYMTDGMYNAAVSEEETAQN
                     QAYKGFVVNFEFKDAQIYIDKTNDKVICYVNYTNDLLQKKNSTEGAQTGVDNNTIIQL
                     TYNKVDGKYLVNNMSTLLITDSQDPTASQNSYGDVIASNDE"
     gene            18678..19064
                     /gene="ssb"
                     /locus_tag="VBE34_001059"
     CDS             18678..19064
                     /gene="ssb"
                     /locus_tag="VBE34_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO7263785.1"
                     /translation="MLNQSIITGRLTKNTELRKVNDRSVISFTLASSRDYKTEDGSYP
                     ADFIDCVLWGAPAESFNTLTAKGDTLQVTGRLQTRTYKDKKEVTHKVTEVQVDKWYIV
                     AVNQNQGDYPVKEKTIVNDSLDNLSL"
     gene            19080..19355
                     /locus_tag="VBE34_001060"
     CDS             19080..19355
                     /locus_tag="VBE34_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263786.1"
                     /translation="MNETTFGTEQHTSKSARRQKSTSDRLKDKLKQLNKAKASLEAAQ
                     KKIKQLEADIKELEAKRQQEILKEYGMSLSDLEAFLADNKDKLGGDA"
     gene            19355..20164
                     /locus_tag="VBE34_001061"
     CDS             19355..20164
                     /locus_tag="VBE34_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263787.1"
                     /translation="MFRITNLSNGQQRDFQNREELEFYLEGEENRCLQLNTTATFQLF
                     HLDKNGELLESMELTIPSSGDQDVKELLGEFGLKKDKKFFWSRGSKQVNVQKVAEKPT
                     KKIKAKNKLTKPTSSARVTHTSTSHHHFWTMIALLFSIFSLAISLTNHYNNKVVDKPK
                     IEKTTINQGADVFCRYFISNYFANSSAREDFISNSLNLNQFDTDKATTVSVLLEKEVM
                     TKKVTTLTYVINCRYDDNTTANKRLTLTVKQNKEAKYGYLVSKAPELTAYP"
     gene            20179..20670
                     /locus_tag="VBE34_001062"
     CDS             20179..20670
                     /locus_tag="VBE34_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3801 domain-containing protein"
                     /protein_id="HEO7263788.1"
                     /translation="MEQEQILDRGARVTLETGKQLFHFLAYAAQQLYKAYDEQKLVGQ
                     QSWKNFNQSSLTKDHIDFLEADVNLKKFTDELKKSGVRFAFKDLGDGTKQVWFEAPNR
                     EVIADALRNTLNEIINDPKKAKEKYMKSEKELTPKEQINKIKKNTKEKLDTVQTKKKG
                     KSI"
     gene            20670..22736
                     /locus_tag="VBE34_001063"
     CDS             20670..22736
                     /locus_tag="VBE34_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEO7263789.1"
                     /translation="MKLQKKPLIPYLIISLVLAFLTHRAYALYSIAPKPDMTNLFSQY
                     TFVLEKYVEPPYFYPNFSPFAILAAMVGFFIGMMFYLKIKPDENYRHGEEAGSAKFAI
                     AQEIAGFKDPEPTNNMIFTKNAQMGLFNKNLPFQWQLNKNVLVVGLPGDGKTFTYVKP
                     NLMQMNSSFVVTDPKGLLVRETGKMLEEHGYQVKVFDLVNLTNSDMFNPFNYMKSELD
                     IDRITEALVEGASENEHKGEPFWDKAQLLLNRALIGYLYFDSKTRDYIPNLSMVAELL
                     RNIRRPNDKEPSTVEKMFVELEESMPGNYACKQWNLFNDNFDAETRTSVFAMVATQYS
                     VFDHQAVTDLIKSDTMEMDTWNTEKTAVFVAISETNKAFSFLASTLFTVMFDQLTHNA
                     DAIIQGQKDGYTADDLVHVQFIFDEFANIGKIPHFNEVLASIRSREMSVKIIIQAISQ
                     LDTIYGDKARKSMVNNCATLLFLGTNDEDTMRYFSMRAGKQTITQKSYSEQRGQRVSG
                     STSTQAHQRDLMTPDEIARIGVDEALVFISKQNVFRDKKAMVTDHPMKDELSNNPNDE
                     KWYDYTRFMVEDPELFNNARDGKIDPKNIWYPDMKGYDQFVKDNDIDTSKLTEIEAAQ
                     ADEASTQATEEPVYEQVPVQLPEMTVESQSQTQESEIKAVDFATGEIFELPPEDEEDD
                     EYYGEV"
     gene            22772..22915
                     /locus_tag="VBE34_001064"
     CDS             22772..22915
                     /locus_tag="VBE34_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263790.1"
                     /translation="MATSKQSPFFKTLGISEDELLHYLKEHPDDPLVTKLEQLLLNLS
                     DKK"
     gene            22992..23426
                     /locus_tag="VBE34_001065"
     CDS             22992..23426
                     /locus_tag="VBE34_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263791.1"
                     /translation="MRALKKLIEGNLSSLGLIELIDDRHDKFGGYYLLYSLSLTAITP
                     EFSESGTLDIEVSFNDYQELTYELGKYSTPFYVNSKDELKCLLEGIVDLPLPFSVSYK
                     PIKEVTWDRYIVSIDGVETTRIIELVKMIKVLEDYLSQVLRG"
     gene            23445..23765
                     /locus_tag="VBE34_001066"
     CDS             23445..23765
                     /locus_tag="VBE34_001066"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263792.1"
                     /translation="MKLFKRKHPIKQELKPEIDVENLIGIGYVRHPNFDNMYRQYTSE
                     QLEELIASLKETIRFLEYQKSLSVAFHEGQTQEVQHLLENKIEGNLSYLRKDEGWLLR
                     ILKR"
BASE COUNT         8462 a         3903 c         4601 g         7049 t
ORIGIN      
        1 ttatggatta attgatgaag ttcgacaagg tgttacaaaa gcaaatcgta tttatgttaa
       61 aaatattatt actgatatta atattctaaa tatggatttt gatgaagcaa aaccttttgt
      121 aaatgcagta aaatcaactg aagtcgggaa attcgacttc aagaaatcgg gaaatccgac
      181 ttcaagaaat cgggaaatcc gacttcaaga agtcgggaaa atcgactcaa gtaagaataa
      241 agagagtaag aataaagaga gtaagaatat tagttctaga aagactgaga aaaattctaa
      301 cgaattttca cagtctgctg aaacttatca gtcatcatca aaaaatcaat caattccatt
      361 cgtcgaacaa aaatattatt cgctcttaca aataattgct gataaataca atgatcaaat
      421 gtttggtttt ccagatgttt tgacaatgac tcacaagcaa aaaatgcaaa ttggtcaata
      481 tttagcagaa ggttatgtta caagcactga agtattaaac atgattgaac gtattccgaa
      541 ggatagtgca tcaccattag cctatttgct aaaatctttg gagaatttga agcaagaacg
      601 tcaatttgaa caaaaaagaa tggctcattt aaacgcggaa aattactaca gtatgaaaaa
      661 agaaggtatt gaaaatggtt aatttgatct tagttttaat tgcagcagtt attgtattta
      721 tctttgtttt cttagcagtt gaaattcatg ataccccttt aatggctgac gatgagatga
      781 cagttatatc atcacccaaa cacgatcacg atgagcatca ttcacaaaaa gctgcttaat
      841 attactcttg acccaagcaa gtctttaaac tacttgatta gatctttgat aatttaacac
      901 aaacaccgac ctgatcagca agcctatagg ataacaatga ataaggaagg cggtgtaaca
      961 gcctgctaaa gtagcaggca cgtcttggct tacctctttt tgaagaggtg tgagtattac
     1021 tcatgcgttt ttgcagatgg aaaaatccac ataagcaaga aaggagtttg ttatgtcaaa
     1081 cttgctacaa gtatctgata aaccattttt aacattatca gatttacgtc ttttaggacg
     1141 tcaaggtgaa gcgatggctt atcttgacga tggaagacaa gttattatca aacctaaatt
     1201 tgccgttgtt cagaaaaaaa gaacaattaa cggtaaaatc acaattgttg gtgaagatat
     1261 tttaaggttt catgaaattt attcagctat taaattcatt aagcgaaaac actttatcat
     1321 cgctgaacga gttaaacgaa acggaaaata tgtcctcgtt ttaacaggaa gaggttatca
     1381 tcgacaaagg aaagaataaa ggagcaaaca tgaaaaaagt aaaagcgtta gctttatttt
     1441 caacagctat tttagcgaca gcatcaacag gagtttatgc agatgaagtt atttcagatg
     1501 ggaccacttc tccatcaacc gagcaagtcg atccaacacc tgctccttca tcatcagaag
     1561 taacacctgg aagtgaatcg aatatcccaa ctccatcatc atcttcttca acagaagctc
     1621 ctgaaggaag taaacgagga tctgaaacaa cagaaacaga accaacgcaa ccagctacag
     1681 agcctgaaaa gtcaacagaa acaccatcag agcagcctgg agaggtagaa gtcccaacta
     1741 cagatggtgg taaagctacc gtagttcctg atacaagcgt tccaacgaac aatccaaata
     1801 ttacagcaga aacagctcaa aatgctggag cttcgcaggt cggaacaact tcaacagtaa
     1861 ctggtcaagt tgttcgtgat gtgactcgta gtaatcctgt gacactgtac aacggtgcaa
     1921 gtcttgttga tattcgagac ggtttagtca ctctctcaac aggtgaaaaa gtagctcctg
     1981 aagttgtagg tgttaaatct aatgctgatg gaacttacac agccaaaacc attcaaggtg
     2041 atacagtcac tttaccgcat actggtgaaa aaaattcaac tgttttatca gttgtaggtg
     2101 ccctcatttt atcagttctt ggttttggct tgaaaaaacg caaaaatcaa gaaatttaat
     2161 ttcaaagtgc taaaataagg aagaataaga aaatggcagt taaaggagta aaagacgtta
     2221 tgaataaagc aaaagtagtt acagcagcag cgacagtggt agcaagtttt ggagttgtta
     2281 cccaagtaca tgctgatgac actgatttag cgacagtatc aaataatacg gaagtagcac
     2341 aaaatcaagc aacagaaaat gttacaaaat ctgatgttga tgtagcaaaa gctaatcttg
     2401 atacagctaa ccaagcagtt agcaatcaag aacaagttgt gagtgatgca acacaatcag
     2461 cagataaagc acaaacagcg tatgacaaag ctcaacagga aacaagtgaa gctcaaaaac
     2521 tcgttgatca agcaacacca gaaaatattg ctaccgccca aaatgatgtt acagaagcgg
     2581 aaaaagaagt agcacaagct caaggtgctg aaaaagtggc agaagatgta gtcaacaaag
     2641 cgcaagaagc ggttagcaat caatcttcag ttgttagtca agcacaacaa tctgttaatg
     2701 attcccaagc agctgtagat gcagctcaaa aagatgttaa cgacaagcaa gctatcttgg
     2761 acggtacagg tcaagctgaa attattgcaa atcgtgacaa agcccaaaat gatgttaatt
     2821 cagctcaaga gcaagttaaa caatcacaat ctgatttaac taaagcgcaa gaagcggatg
     2881 ccaatcgtca agaagcgatt gataatgctc agaaaacagt tgatgaagct aaccaaactg
     2941 ttacgtcaac caaatcagat ttagatgcta aaacagcaac ggtaactcag acacaagcta
     3001 ttgaagatgc agctcaatca gctgtagatg cagcacaaaa agatgtcaat gacaagcaag
     3061 caatcctaga tggtacaggt caaaaagcca ttttggatga agctgatgct gctaaagttg
     3121 ataaagcaaa taaagaaaca gcgttaactg atgctcaaaa aggtttgaaa gaagcacaag
     3181 aagctgatgc aaatcgtcaa atagcaattg ataacgctaa aacagccgtt gatacagcaa
     3241 atcaaaatgt tacttcaaca aaatcagatt tagatgctaa aacagtaaca gctcaacaaa
     3301 ctgaacaagc tctaaaagat gctgaagctg catataaaac agctgaaaat gattataaag
     3361 ctattaacaa aattactttg actcaagctt atgttgaagc gttgaaaaaa tataatgatt
     3421 attcattgtc ttctgaagaa cgtcaacaag tattgaaaac attagttgaa gaatcagaag
     3481 ccttagctaa attgaatagc tataagagta atcctaacga tgataattca acaaaatatc
     3541 aaattaacga tcttccagaa gatgttatta aagaattatc tcaatttgct gctgatttag
     3601 ttaatcaaat cagaaaagct ttcggaacac cacaagtttc agttactagt tcatctgtaa
     3661 aatttgcaga tcttgttact gatggatatg tttcagatgg ttggaatgtt acaaaagcta
     3721 taaacgaagg tcaagttggt catgatgcta aagctgtcaa taatgcagct cgatactttg
     3781 gattgccaac ttcaccatct gacgatgaag caacaggtgg tcaatattat gaagattggg
     3841 cttcttcagc agtttatgac tgtattacat ttgctacatt aaaacaaaga gtatatgaag
     3901 ctgtcacaga ctttatgttt aatgcggatg aatggcgcca tgctcgagat attgttggcg
     3961 gtggagatgt caattctcat gtttactttg ctatcgactt ttcaaaacct tcatcagaaa
     4021 atggagcttt tgctggtgta cactttattt ctgttgcgga agatcaacta gtaactaaaa
     4081 ataataattt tgatacaatt gctattgcta atccaaaatc agctgaaaat gtaactgcca
     4141 cttacaatgc agctaaagat agttacaatc aagcaacagt agctaataca gcagctcaat
     4201 cagcaaaacc aacagctcaa gcagcatacg ataatgcaat ttcacaatta tcgtcagcaa
     4261 gttctgcttt aacgcaagtc caatcagtgg ctgttcaaac tccgactgct caagctaatc
     4321 ttactttagc agagacagct ttaactaaag cacaagaacg tttaacaaaa gcaactcaag
     4381 cagtagaaga tttgaatgct gatattaaag ctaaacaaga agcattaact tcagctaaag
     4441 cagttcttga aacagcccaa tcaactttgg cacaagccca atcaaaaaaa tcaactgccc
     4501 aacaagctaa agctactgct caagcagcat acgataatgc aatttcacaa ttatcgtcag
     4561 caagtgctac cttaacacaa acacagtcag tagctattca aacgccaact gctaaagcta
     4621 accttgcttc agctcaatca acattgaagc aagcacagga aagtcttgtt aaagctcaaa
     4681 aagcagtaga tgatttaaat gctgatatta cagttaaaca agctaatcta gcatctgcta
     4741 aacaagttct ttctaccaaa caagcgacat tagccactaa ggaagcaaat cttacggctg
     4801 aacaagctcg tttagttggt ttgcaaaata gtcttgctac tgctcaagat aatgttagta
     4861 gtgctcaagc aaatgtcgta gctacaaaag ctaatttaga caaagctaaa cattatttga
     4921 caagtttgca aactgcccct caattattag ctgatgctaa aaaacaagaa gaaacagcaa
     4981 gaactcattt gctagatgct cttgatacgt tagaaacaga attacttaaa ttaaaagact
     5041 tacaagttaa acaagcagta gcacaagcgg tttatgatac aacttcaaaa gcttatcaaa
     5101 ctattctgga tgctcaagaa aaacaacgat tgcaagatga atataatgct attgttgctc
     5161 aaggaaaaac tccagttccg attgttgata aaactggtaa gattttaggt tatcgagttg
     5221 aagatcctca agtagaaatt caatcttcaa cttcttcatc atcagttaaa gcagtagaag
     5281 aagttccagc tgttaaagct agtgtacttc cagaaactgg tgaaaatagt tctattctta
     5341 caatccttct tggttgtcta atgacacttt ggggattgat tgaagtacgc cgtaacaaag
     5401 ctaaataatg tcaaagcaac tctatggtta ttaatcatag agtttttgct ttattcataa
     5461 cgtcttttac tcctgaccta agcaggtcga taaactactt ttccttattc ttttcttcaa
     5521 tttttaagta gacccgaaca tgtcaataaa ctgttcgttt tttattgctg taatttttta
     5581 ggaaaaacca ggaggttatt atgtcaaata aatttattca acaatcacaa atcaaaaagg
     5641 gacacggatt tttccgcaag aaaaaatggg cgaaaggtct agcttcaggg attgctcttg
     5701 gagcagtcgt tacattttca agttcagcat atgctgatga tatagcgaat acagttgata
     5761 ctcaaccaga ggtaacaatt gtttcggata gtaatgctac taatttagca gatgctcaag
     5821 ctgataacag tcaagagcac actggtttaa ccaatcaatc aggtgagcag acaggtcaaa
     5881 tgactgacaa aataacatca gatggtttag atcaatcagt ctctgatgct caaacagcag
     5941 gagttgaagt agatcaaaat ggtactgtaa ctcattctag ttatgcagat gctcaagctg
     6001 atttagccaa gcaaaaagaa gccattgacc aagctgttga aacacaaaaa gaagttgaca
     6061 atgctaaagc aacagctgaa gaagcagcta aagcagcagg tgttgaagtc tcaaccgaag
     6121 caagcaaatc agcaaaaacg tatacttcag ttgatgatgc caaggttgat gctaaaaaac
     6181 aagtagataa tctttctact gtgactgaag ctcaaaagca agtcaatgaa caacttccta
     6241 aagcagtgga agcagctacg gggtcaggtg taaaagtgag tgttaaagct ggtgctaaaa
     6301 caactgatgc taaaaaagct cttgaaaatt tagcaaatca agttgctaat ttgaaaaaag
     6361 ctcaagatac tcaaaacacc attagcaatt caatggcaca agctcttgaa gaagctaaaa
     6421 aagcaggtgt tacagtcagc acaactggtt caaaatcata tgatgatcta tctaaagctt
     6481 tagcagatgc tcaagctcaa gtcgctaaac ttactgaatt taaagaaaca caagctagca
     6541 tcgatgaagc tattaaaaaa tcggctgata atgctaaagc ttcaggggta acagtttcaa
     6601 ctggagatgc aaaaacatac acttcagcag cagatgctca agctgatagt gataaacaaa
     6661 cagcaacttt agataaagca gctgaaactc aaaaagtagc tgataaagtt atttcagatt
     6721 taactaaaga agctcaaaat gctggcttag atcttacaaa aggaacatca caaacataca
     6781 cttcagttga agaagctaaa aaagatattg ctaaacaaga acgagctcta aaagatgcta
     6841 caacagccaa agcaaatgct gaaactacta attcatctaa caaaaaagct gcagcagatg
     6901 ctcaagcagc tgcaaataaa gctatcaatg atgctattgc taaggcaact gcttctggaa
     6961 caacagttac taaaactagc actattgaaa caactgtaga agaagcgcaa aagattgcta
     7021 aagagcaatc tgataaagtt aatgacgttg ctgcaaaaaa cgaacaaatt caaaatgaaa
     7081 atactgcaaa gaaaaatgcc tatgatgcta aaaaagctga agtagacaaa aagaataaag
     7141 cagcgtttga tcgtgctggg ttgaattaca ctggtgatta tgcaacagat aaagcaacag
     7201 ctgataaatt taattcagat gaaatcgaac gagttaaatc tcaatttgag aaagctaatg
     7261 acaacaatgt acttggtgca actgatggtt gggttcaaca cgttggatca aacggataca
     7321 ctcaatcagg tacaagtcaa ttcaagaagg ttcaaaacgt taatggtgta gaaattgttg
     7381 caaacggttc tctagaacca gggaaagcaa ctgtttacgt taaaggtaat gttaatcaat
     7441 ctcatgttgt tgcaaatatg aactggggaa atgttgcgcc acaaggtgat aatcttacta
     7501 gtaacattgg ttcaaaatgg gctgaagcat acgattataa cgcagatggt acaacgactc
     7561 aaatttggaa gggtaaaatt ctaaagtggt atcgtattcc aaatgctatt actatgcttg
     7621 atggctcacg acacgatgcg tacgtactgt tccatactga tacttcaggg ctttcatcac
     7681 caggagatga agttgttttt tggaatcaag atggtgctat taatgctgta gacggttatc
     7741 gttacggagt taacgataaa tctgatggta ttcgtacagt tattcgtgtt gactcaccag
     7801 ataataacga taattatctc tggatttccc ttttaggaga ctttgatatt ggtcaattcc
     7861 tagaagcaga cggcgtaact gtccttggtg ttggcggtgg atttgcaaca aattcaggtg
     7921 ttggcggaca ccgtgttgct gtcgatgaaa acttaggact tacttatggt aaaaatacaa
     7981 ctccaactag tgcattgact ggttttaact cagctcccga tggcgtcgct ctgattgcac
     8041 aatatagcaa tcaatattca actgttattc gaaatactgc tggtggtaac ggtgttgccg
     8101 ttgctcgtac tgattttggg gcacaagcaa aagttagtat cgtaacacca cgtcatgtga
     8161 atgttgaaaa tgttgaaacg ccaacatata cagagttatt accagtttca gtacaagatg
     8221 tacatattac acctggacaa gtaactgtac ctaaagctgt aactcttcac gatgttaatg
     8281 ttcaagctga aactcacgct atcaaacttg ctactgaagt tgctccagtt actgttgaaa
     8341 cagaagtaac gacaccagct ctttcagcaa ctgttcatga tattacactt aaaacagcag
     8401 ttcatcctgt tgaagtaact caaaaaccaa caaacgaaaa agcagttact aacaatgaca
     8461 atgttgatat taatggtaaa actgttgcta aaggctcaat agtaaactgg gtactaaaaa
     8521 atggtaacct cttagcaggt cgtgaagaat tgactgctgt tgtgatgaat gatccattcc
     8581 cagcaggttt tgaagtagat agtgaagcca ctgctaaagc aaatgctgac tattatacac
     8641 ttaaaacaac cgaaaatggt tatcaacttg caggaacagc taaattattg tcacttttga
     8701 acgctcatcg tgatcaagat gttgaagttc caggttttgt ttttgttggt aaaccactta
     8761 atgataatgc tcattatgat aatgcttttg aaacgattta ttcaaccgct aaaggttatt
     8821 ataaagttgt ttcaaatatt ccttcaattg acactccaga taatccaaaa ccaactaaaa
     8881 ctgttacaga taaagcgggt agtgatattg atggtaaaac agtatttgat aaagatgtta
     8941 ccttccattt gattacagat tatagtactt acgttaatac agttgttgat gttaatacaa
     9001 caacaaaagc agcaggtatt tatgatgata ctgatgatgc gactgtcaaa cctgacgtaa
     9061 caaaagctac tgtaacagat agtaaaggca atgatattac tgccaaagga acattttatg
     9121 atattgaagc aggtcaacca attccaactg aaattcaaac tttcttggat ggtgcgaacc
     9181 ttactccaaa tggtgaattt attgtatgga ttccaaatga tttgaaagat tactacgaaa
     9241 attatgttct aactggtaat aacgttactg taaacttacc agttgaagtt attgctcaac
     9301 caggcgaaaa agctgaaaac acctttgtgc aagttgaatt tggtaatggt tatcaatcaa
     9361 atattgtctt tgtaaaagtt ccagatgttt caccagaaaa acacgctatt agcaaagatg
     9421 gtacagttct tgatggtcaa gaagtcggac ttaatcaagt ctttaactat aaactggatg
     9481 gtgtgactgt tccaaaagat catgacacac tttggcaata tgatggtaaa gataagttag
     9541 atattgaaca tgaccgctac acaggaaact ggaaaggtgt cattactggt acagagtata
     9601 ctgccaaaga agatatgacg ttaacatacg atgttattac tgaagatggt acagtcatta
     9661 aagcaggcga tactatcgca gctggaacaa actatcattt tatctttgag ttcaatcaag
     9721 atactaatga tgatttcatt aataaaattg ttaaggttac ttgggatgct caaaaaggtg
     9781 aatgggctta tagcattgat gaagattttc ttaaatcact aggtgttgaa ggtactttcg
     9841 atgctgactt ttggattgaa gtggaacgta ttaaagatgg tgaagttgaa aatactttca
     9901 tcaacactat taatggtcgt gagttagttg ctaaagtaac aactcataca ccaacaccag
     9961 aaacacctgc taaaccagtg gaagaagttc cagctgttaa ggctagtgtc cttccaacaa
    10021 ctggtgaaaa aactacagca ttaaatgttt tagcatctat cgtaggtctt gttatgactg
    10081 ttggtgtagc gtttggtctt cgtagaaaag aaaaacataa ctaattaaaa caagaacagg
    10141 cattgcctgt tcctagaaga gtattatctt gcctaattaa tgttacgtct aaatctccag
    10201 ataatactct tctaggagca ggtaattgcc cacttggttg gaaaaggctg taaaaataac
    10261 tgataagact atttacagcc ttttattttt attaaacatt ttatttaatt aaacatttta
    10321 tttaaaggag aaaatttatt tatggacaca gcagttcgtg tagttacagc tctaggagct
    10381 attttatcaa tcatcggttt gggctgggta ttaacaggag cctttgatta ttttgcaggg
    10441 cgtaaaaatg gtaatccggc aatgatggac caaggcatga cagcaatggt ttcaggtgga
    10501 gctgttgcag ttatttcagc aggtgttgca gctgcaattg ttgcggctat gcactcaatt
    10561 tcattctaat aacttacata catgtaagat aatcagttga aaaagtggta taataaaggt
    10621 aagtaaaagg gttacaagga gaaaagaaaa tgaacaagga atttgattat gtagcttttg
    10681 caaaaaactt tgaagccact aatggacgtc caccaacagc ggatgaactt gataatgcta
    10741 aatgggaaca ttttggtatt agatggcata cgccagaaga gtcacaagct gagttggaac
    10801 gtattaacgc atcttataag ccaacattgg gcgaacactt aaaggatgct ttaggctttc
    10861 taggacgtgg atttttcaaa gctttatttt ttgttcttat gtctccacta tatttaacaa
    10921 tgcttttctt taatttaatc aaatcagcac tcggtgtatt cttggtttgg tttgtttcta
    10981 aatttatgtt tctgcttgtt attgggatga ttgcagactt aagaggcgag agtgaaatta
    11041 ctggcgtttc aaaaagtcta gttgattgtc tttttggttc agattttata tctacaggaa
    11101 atccgaattt tttcccacac cctgtattag atgcttggat tataggaatt gtcattgttt
    11161 tacttgcatt agttgccact ttttcaaaag tagaagaaca caaataatta taggatgaga
    11221 accttatttt caaggctctt ttttattatg ctagaaagga gagatatgga taaaattgtt
    11281 tcggaattga ctgagacttt aggaagttat aataaaacag ttaattcatc aattggtgca
    11341 atcaacaagt ctttgatggc aataggttat attctgattt cgattttatt tttaattgaa
    11401 atgctctctt ggtacagatt tattcggaac caaggtggtg agatgacttg gaagcttttc
    11461 ttagaagtgg cggttaaata ttttattgcc tactttttag tagctcaatc aggtgcagtt
    11521 ttaaatgcaa ttatgtggtt tactaatggt attaccaagc tgattggtgt tgattttaaa
    11581 ggtgcaggtt ttcaatatgt tccgattaaa cacggtaaat atggaattag attgatgatt
    11641 gacggtattg gttttattgt tggtgccgca gcaactctat ctttaaaagt aatcgttcta
    11701 ttgcgtttca ttgaacttta tttctttaaa gcaatagcac ctgtaatcgt tgctttttgg
    11761 atgagtgata atacacgtca tattgctgtt aactttttaa aaatgtttgg agcagttgct
    11821 cttcagggtg ctgtaataat tctattgctt gccatttggc aaggatttag ggttgataca
    11881 ttagttcaat tagataaaaa tagttggtta ggagcatatg cttcaggttt tagctatata
    11941 ggtaaatgtg tagtattctt aattttattg attggttccc aacgtaaagc taaacaattg
    12001 ttgaatgtat catagaggta aaaatatgaa tgatttattt ttcgataaac ctaaaaatag
    12061 atttagaaag agggaattat gaagaataat attgaaaagc ttattaaaga aagtcaaaaa
    12121 aattaacaac gattagtgag gaaactggga ttgcttatcc aactttgtcg ggatataatc
    12181 aaggaataag aacaccgaaa aagaaaaatg cccagatact tgcagattat tttggtgtat
    12241 ctgttccata tttacttggc ttggacgata acccgccagc attaaatgac tcattagaga
    12301 aaataatggt aattcatgaa gtgctaaatg caggagataa tatcttagtt gctcaagagt
    12361 tgttaaaaaa ttccttagag actttagtta agtatgctga taacgattat agggtgcaat
    12421 tcaaacatca cttactgata tcaaatactt tatcagaagt cataggaaaa ttagatattg
    12481 ttactgaaaa cgatgaattg aattttacac cagataaatt gaatttatct aataattaaa
    12541 tgttagagat aacaaaaaat taatcgaagt ttatttaaag attatggatt atgtaagtga
    12601 aaagcagttc tttaaaaact gctttttttg atggaaagga gaaaaatgaa caaattaggt
    12661 tctgagtttt ttaaagccat tgatcaattt gaacgaagtg ttgttggtgg ttttactttg
    12721 agacaactca ttatgctaat cgggattgtc attggtgttg ctttagcaac attgatctct
    12781 ttcttgaaat tgcctgatat tttgttttat ctggtattag ctttgacggt tccaccatca
    12841 tttatctatg gtataaaaaa agacgaacaa attaaagaag ttttgatgtt taaatttaca
    12901 gtacaagaac gtgcttatca aacagatttt gaaagtgagg atatgaatgg tacgtttatt
    12961 caacaaaagg gcgtccatga atggaacgac acctttgact aaagaagata aagagcgagc
    13021 aaaacagctc aaaaaagctt tgaaagctag tactcaaaac acaattaaat atacgtcttt
    13081 gtttgaggac ggtttgatgc acattgttga tgaggagtac tcaaaaacct gggaacttgg
    13141 cgatacaaat tatctcacgg cagatgaaga tgaaaaactt gatattattg attattatgt
    13201 tgaagcttta aacggcttag atagtgataa tacttatcaa ttactaatta ttaatcgtcc
    13261 tgttccgtca acacttttaa gccaaattac ctatgaactt gaaagtgatg accgtgatgt
    13321 ttatcgtcaa gaatacaatg atatgattac atcacgtttt gcaaccgacc aaaataattt
    13381 caaggttgaa aaatttatca cggtttcaac ttctgcacga gatcgtaaac aagcttatcg
    13441 taaattgaat gatgttgaaa acaatttttc aaaacaattt caagtgattg atattccttt
    13501 tacgcctttg acaggaacag agcgattaaa tatttttgct gatttgttac gtggcaatcc
    13561 ttatttgaat gttgattata aagatgtacg tattagtggt ttaacaacaa aatcttttgt
    13621 tgctcctgga cgtatttggt ttcaacacca tcaatttatg atagataaga aatactgtaa
    13681 agttcttttt gctagaagtt ttccagcttt tctaaatgat cgtctaatca agtcaattac
    13741 cgacattggg attgaattag cgattacaat tcatgctaaa ccgtatgacc cagcagatgc
    13801 tgttcagaaa atcaatacag ctgaggcagg ggtaaaaatg gatatgatta agtctcaaaa
    13861 ggttgctgca gacaaaggta tttcaggaaa tttagcagta tcaggtgtag ctcaagctac
    13921 tgctgaagaa gccgctaaat ggagtactga aattaatgat aatgaccaaa aaatgttctc
    13981 aggtattttt gcagtcatgt taaaagcaga tacacctgaa gaacttgctg attatacaaa
    14041 ccgtgtcaaa caagctggac gtaagcatgt ggttgatttt gaagaaactt actaccatca
    14101 agaagaagcg cttaatacta ttttgccaat tggaaaaact tatcttgatg ttaaacaccg
    14161 cttcatgcgt gatatgacaa caacaaacat tgctacacaa attccattta cgaatactga
    14221 tttgcaaagt agcagtccta tggctatcta ctatgggcaa aatcaaattt caaacaatat
    14281 tattacgctt gaccgtcaac gtgatttgaa cacgtcatct ggtgtcattt taggttcttc
    14341 aggttctggt aagtctgttt ttgttaaaac cagtgaagtt atcccaacta ttttgaggta
    14401 tcctgatgac cgtattatta ttgtagaccc tgaagatgag tattcggaca ttggtcgtgc
    14461 atttgatgcc caactaattg atattttccc aggttcacag acacacttga acttgatgga
    14521 tttaccagat gaagaaaatt tatcagctga agatgctgat ccgattggtg agaagtcatc
    14581 acttatcatg gggctttttg aaaatattct gcaagaagtt actgatgaag acttctcaat
    14641 cattgaccgt gtgactcgtc tttgttatga acgaattact gatagaacac caactttgaa
    14701 agattggtac gacattttag ttgaacaacc tgaagaagtt gcccaagatt tagcattaaa
    14761 atctgagtca tatacgaacg ggtctcaaga tatttttgct catgaaacta atgttaactt
    14821 gaataatcaa gtggttattt tcaatctgaa aaaattggat ggtaagttaa aaccatttgc
    14881 tttaatggtt gtacaagatt atatctggaa ccaggtcgtt aataatcaag gtaagctaac
    14941 aactcgtatc tactttgatg aaatgcaaaa tcaattctta acagataatc aagcagagtt
    15001 ctttactaac ctttacgccc gtgttcgtaa gtatggtgct attccaacag gtatcactca
    15061 aaacgttgaa actttgttga atcgtactga aggacgtaag ttgatttcaa acagtgaatt
    15121 tattgttttg ttgaagcaaa agaaaacaga cttagcttat ctaacacaaa ccattaattt
    15181 gacagatgca cttattcgtt atattgaaaa accaaaagca aaagggacag gattaatcat
    15241 tgctggtcaa acggcagtac catttgaaaa tccaattcct aaaaataccg aactattccg
    15301 cttagtggca acggatgctt accgtcaaat tgattaatca atttatttat caggatagtt
    15361 agaaggagag ggggtgaatc atgtctaaca aagatgattt aagaaagcgt gtggttagaa
    15421 aacgtcatat taaagaaagt gttcaacgtc ataatcagtt gtcagctaag aaagctcaaa
    15481 atgctgctaa aaaagagcat cgtgaagcta agattaaggt gaaacatgct agagcagctt
    15541 taagaagagc tgagaaaggt attgataaag atgttgatgg tgaaaagatt gtagacgact
    15601 tagagaaagc gctagctgaa aagaaagcta ctaagaaaat caaaaagaaa gtcagaaaga
    15661 ctaatccaac catcggtcag aaagctagac ggactggtaa aaacattgca ttaaatgcta
    15721 gtcatcaaat taaatctact gccatggatc aagatgatac tttgtcagat ttacaaaatc
    15781 tacagtatcg aacaagacaa tcaaaagcag ttgctaagca ggctggtaag gtgacttggc
    15841 gaggagctaa actagcttat cgaaaaagca aaggtatggt aaacgttagc cgtggagctt
    15901 atagaagtgc taaggatagc gctaaatttg ccactaaaac cactgctaag gttgctacaa
    15961 aagtagcaca gaccactgct tcagctgtaa aaacggcaac aagtgcagca gcaactatat
    16021 tagctaaccc tatgacgtgg attgttagtg ctgttgttgg ttttacttta tttttggtaa
    16081 tccttgtatc ctcaatcttt tcaagcaatg ttattcaaca aaatgatttt acacttaatc
    16141 aatcctggct tcatatttca caagttgacc gccaaaaatc atctgaccaa gttgaatatt
    16201 acacggatat tgattctatc ttgctttaca tgaactttcg ctatggtggt gaatgggaac
    16261 cagatgctaa ctgggatgat gggacaggtg gtaaattgac aggagcgctt gggtttaatc
    16321 atttttcgga tgcgctggat gatatttgga aagaagaaaa tagcgatatt gataacctaa
    16381 aaactatggc tgagctttat actaacggta aagcttggat gaagttggat aaagatgact
    16441 tagcagatta taaagaaatt cttgagagcc aatccgaaac aggaaaatac ttagctactc
    16501 aagaattgat taatccattt tattcggcag atgatgctaa gagcgataca gattacctta
    16561 cgatttctaa gcgatatggt tatacttcaa aggataaaat cgatccaaca accacacttc
    16621 aagctcagtc aggtcaaaag ctttatgcag tcatggatgg taaaattacc gttacaacaa
    16681 aagatttgaa gggtaacgaa actaaaacaa ctaatttaat tatttcagat aacgatgctc
    16741 gttttatttt ttataacata tcagctattc gagttaaaac aggtgataag gtagaagcag
    16801 gagccgagct tggaacagtt tctggtggtt cacaaaagat tgcctatgcc aaaaactatg
    16861 gtgaagttga tagtaaagat tctcaatggt tacaagacat aaaaaataaa gatgtttcat
    16921 atggatatac ggagcagaca aagaaaaata agacaaacgt ttgggtgtta gttaatcctg
    16981 gtttttattt cccatttgtt aagtacgctc aaacaactgt cgttatgcga acatcatctg
    17041 aattgagtgg acgtgctaaa caattttatg acttgatgaa gaagttttat ccaagcgcta
    17101 ctgataatgg tattgctgca gtggttggga actttgccgt tgagtcatct attaccccaa
    17161 aacgagctga aggtgattat cttaaccctc ctattggtgc atctgatggc tgttgggatg
    17221 atgttaattg gttgaatatg ggtggtacag cgatctacgg tggtggttat cctaatatcc
    17281 ttcatcgtgg gcttggactt ggtcaatgga cggataccac cgatggcggt actcgtcata
    17341 ccatgttaat caattatgct aattcaattg gtaaaaaatg gtatgagcta gaaacacaaa
    17401 ttcgatttat gctagaaggt gattcacctt actatcaaaa cattttaaaa agtgtgttaa
    17461 cctctaattc tgatacagat agtcttacca gaaccttctt aacaaaatgg gaaggtatca
    17521 acaacggaac actttcgcaa cgtcaaagtt atgccaaaca agcgttatct tggtttcatc
    17581 aatcaattgc tggtggtgga acaatggctt cctcttggaa ttttcctgaa gagtatcggg
    17641 ataaaattaa gagtatgcca actggtgtta gtatgacaac acaacctggt agtggttatc
    17701 ctgcaggtca atgtacctgg tatgcttata atcgtctggt tgaattaggt gaaatcacgg
    17761 atttatcagg aagttatggt caccttggcg atggtaaaag ctgggtagga aatcttgtcg
    17821 caaaaggttg gaaatttagt acgatgccaa cggcaggagc tgtttgttca acagctggtg
    17881 gctttgatag cacttatcca caatatggac acgttatgat tgttgaagtg gttaatgctg
    17941 atggtagttt tttggtatct gaatgtaact atgcaggtaa caaaagccaa attcactggc
    18001 gtgtttgtcg taacgctccg tactacacgt ttgcaacccc ttaaaaatga aaggaaaaaa
    18061 atgatagatg aatttaaagt gacttttttc aaacgggtca tgatgattgt agggattatt
    18121 attatctttc tcattggctt aggttttggg tcaatgagca agaaaacaga taatcaatct
    18181 tcaagtcata caactttaac caaaaaggtt aaagaaggtc atcagttgac tgagggttgg
    18241 gttaagcagt ttttgattgc ttattacact aaaaaagatc ttgaagaaaa tcgtaacaga
    18301 tacaaagatt atatgactga tggcatgtat aatgctgctg tttctgaaga agagacagca
    18361 caaaatcaag cttataaagg ctttgttgtt aattttgagt ttaaagatgc tcaaatttat
    18421 attgataaaa ccaatgataa agttatttgc tatgttaatt acacaaatga tttacttcag
    18481 aaaaagaatt caactgaagg tgctcaaaca ggcgtagata ataacactat tattcaatta
    18541 acttacaata aagttgatgg caaatatctc gtaaataaca tgagtacgct tttaattaca
    18601 gattctcaag atccgacagc ttcacaaaat tcttatggag atgtcattgc tagtaatgat
    18661 gaataggaga gaaggcaatg ttaaatcaaa gtattattac aggacgtctg actaaaaata
    18721 cagagttaag aaaagtcaac gaccgttcgg ttatttcttt tacacttgct tcaagtagag
    18781 actataaaac agaggatggt tcttatccag ctgactttat tgactgtgtc ttgtggggag
    18841 cacctgctga aagctttaac acgttaacag ccaaaggtga cactcttcag gtaacaggac
    18901 gactacaaac tagaacttat aaagataaaa aagaagttac tcataaagta actgaagttc
    18961 aagttgataa atggtatatt gttgccgtta atcaaaatca aggtgattat cctgttaaag
    19021 agaaaacaat tgttaatgat agcttagata atttatcact ataaaaggga gaaaagaaaa
    19081 tgaatgaaac tacttttgga acagaacaac acacttcaaa atcagcacgc cgtcaaaaat
    19141 caacaagtga tcgcttaaaa gataagttga aacaacttaa taaagcgaaa gcatcccttg
    19201 aagctgctca aaagaaaatt aaacagttgg aagctgatat taaagaactt gaagctaaac
    19261 gccaacaaga aattcttaaa gaatatggta tgagtctatc ggacttagaa gcctttttag
    19321 ctgataacaa ggataaactt gggggtgatg cgtaatgttt cgcattacaa acctaagtaa
    19381 tggtcaacag cgcgattttc aaaatcgtga agaattagag ttttatctgg aaggggaaga
    19441 aaaccgttgt ttacagctca atacaacagc aacctttcag ctttttcatc ttgataaaaa
    19501 tggagaatta ctagagtcga tggagcttac tattccatct tctggagatc aagacgttaa
    19561 agagttatta ggtgagtttg gcttaaagaa ggataaaaaa ttcttttgga gtcgtggtag
    19621 taagcaagtg aatgttcaaa aggtagcgga aaagccaact aaaaaaatta aagcaaagaa
    19681 taagctgact aaacccacgt ctagcgccag agtaacacac acttcaacta gtcatcacca
    19741 cttttggaca atgattgctt tgttattttc aatttttagt ttagcaattt ctcttactaa
    19801 tcattacaac aataaagtag tggataaacc taaaattgaa aagactacta ttaaccaggg
    19861 cgcagatgtt ttttgccgct attttattag taattatttt gctaactcat cagctcgaga
    19921 agattttatt tcaaattcac ttaatttaaa tcaatttgat actgataaag caacaacagt
    19981 cagtgtcttg ctagaaaaag aagtgatgac gaaaaaagta acgactttga cctatgttat
    20041 caattgtcgc tatgacgata atacaacagc caacaaacgt ttaacattaa cagtaaaaca
    20101 aaacaaagaa gctaaatatg gctatttagt ctcaaaagct cctgaattaa ctgcttatcc
    20161 ataaaggggg agaagattat ggaacaagaa caaattcttg atagaggtgc acgtgttaca
    20221 cttgaaacag gtaaacaatt gtttcatttt ttagcttatg cagcgcaaca actgtataaa
    20281 gcttatgatg aacaaaaact tgtgggacaa caatcttgga aaaattttaa ccagtcgtct
    20341 ttgacgaaag atcatattga ttttttagaa gcagatgtta accttaaaaa atttacagat
    20401 gaacttaaaa aatcaggtgt acgctttgct tttaaagatt taggtgatgg cactaaacaa
    20461 gtctggtttg aagcgcctaa tagggaagtt attgcggatg ctttaagaaa cacccttaat
    20521 gaaatcatta acgaccctaa gaaagctaaa gaaaagtaca tgaaatcaga gaaagaattg
    20581 acacccaaag aacagattaa caaaatcaag aaaaacacta aagaaaaact tgatactgtc
    20641 caaactaaga agaaaggaaa gagtatctga tgaaacttca aaagaaacct cttatccctt
    20701 atctaatcat tagtctggtt ttagcttttc taactcacag agcttatgca ttatatagca
    20761 tagctcctaa accagatatg acaaatttat ttagccagta tacttttgtt ctggaaaaat
    20821 atgttgaacc accatacttt tatcctaact tcagtccatt tgctatttta gctgcaatgg
    20881 ttggtttctt tattgggatg atgttttatt taaaaataaa acctgatgag aattatcgtc
    20941 atggtgaaga agcaggttca gcaaaatttg cgattgctca agagattgct ggctttaaag
    21001 atccagagcc aaccaacaat atgattttca caaagaatgc ccaaatgggc ttatttaata
    21061 aaaatcttcc gtttcaatgg caattgaata aaaatgttct tgttgttggt cttcctggtg
    21121 atggtaaaac attcacttat gttaaaccta atctcatgca aatgaactca agttttgtgg
    21181 ttaccgatcc aaaagggttg ttagttcgtg aaacaggtaa gatgcttgaa gaacacggtt
    21241 accaggttaa agtttttgac ctggtcaatt taactaattc agatatgttc aatcctttca
    21301 attatatgaa atctgaacta gatattgacc gtattaccga agcgttggtt gaaggtgctt
    21361 ctgaaaatga acacaaagga gaaccttttt gggataaagc tcaattactt ttaaatcgtg
    21421 ctctgattgg ttatctatat tttgacagca aaactagaga ttatatacct aatctttcta
    21481 tggttgctga attgcttcgt aacataaggc gtccaaatga taaagaacct agtacagttg
    21541 aaaagatgtt tgtggaactt gaagagtcaa tgcctggtaa ttatgcttgc aagcaatgga
    21601 atttgttcaa cgataacttt gacgctgaaa caagaaccag tgtttttgcg atggttgcta
    21661 cacaatattc tgtttttgac caccaagcag ttacagattt gattaagtct gataccatgg
    21721 aaatggatac ttggaacact gagaaaacag ccgtctttgt cgctatttca gaaaccaaca
    21781 aagcttttag tttcttagca tctactttgt ttacagtcat gtttgaccag ttaacacaca
    21841 atgctgatgc cattattcaa ggtcagaaag atggttatac agctgacgat ttagtacacg
    21901 ttcaatttat ttttgatgaa tttgctaaca ttggtaaaat ccctcatttc aatgaagttt
    21961 tggcatctat tcgtagtcgt gaaatgtctg ttaaaatcat cattcaagcc attagtcaac
    22021 ttgatacgat ttatggtgat aaagcaagaa aatccatggt taataactgt gcaaccttgc
    22081 tattcttagg aaccaatgac gaagatacta tgcgttactt ctcaatgcgt gctggtaaac
    22141 aaaccattac acaaaaaagc tattctgaac aacgtggtca acgtgtatca ggatctacaa
    22201 gtacacaagc tcatcagcgt gacttgatga cacctgatga aattgcacgt atcggtgttg
    22261 atgaagcatt agtatttatc tcaaaacaaa atgttttccg agataaaaag gctatggtaa
    22321 cagaccatcc tatgaaggat gaattatcca ataatccaaa tgatgagaaa tggtatgatt
    22381 atactcgttt tatggttgaa gatccagaac tctttaataa tgcacgcgat ggaaaaattg
    22441 accctaaaaa tatctggtat ccagatatga aaggttatga tcagtttgtt aaagataatg
    22501 atattgatac ttcaaaacta actgaaattg aagcagcaca agctgatgaa gcaagcacgc
    22561 aagccacaga agagcctgtt tatgaacaag tacccgttca attacctgaa atgaccgtag
    22621 aaagccaatc tcagacacaa gaaagtgaaa taaaagcagt ggattttgcg actggtgaaa
    22681 tctttgaact tccgccagaa gatgaggaag acgatgagta ttatggtgaa gtttagcgtg
    22741 taataaagta taatagttcc aagaggttta tatggctaca tcaaaacaaa gtccattttt
    22801 taaaacactg ggaatttcag aagatgaatt attacattat ctaaaggaac atccagacga
    22861 tcctttggtt actaaacttg aacagttact attaaactta tcagataaaa aataagctac
    22921 atcgttaaac ggtgtagctt ttgtttttgt aaaatcgtga tttacttaaa atatataaag
    22981 gagaattaac aatgagagca cttaaaaaat tgatagaagg caacttatca tctctaggtc
    23041 ttatagagtt aattgatgac cgacatgata aatttggggg atattattta ttatatagtc
    23101 tctcgttgac tgctatcact ccagaatttt cagaatcagg gacccttgat attgaagtta
    23161 gttttaacga ttatcaggag ctgacttacg agttgggaaa atattctacc cctttttatg
    23221 ttaacagtaa agacgaactg aagtgtttat tggaggggat agtagatctt ccactccctt
    23281 tttcagttag ttataaacca attaaggagg taacatggga taggtatata gttagtattg
    23341 atggtgttga aaccacacgg ataattgaac ttgttaaaat gataaaagtc ttagaagatt
    23401 acctcagtca agtactaaga ggctaatata taaaggggat aaaaatgaaa ctatttaaac
    23461 gtaaacatcc tattaaacag gagttaaaac cagaaataga tgtagaaaat cttattggta
    23521 ttggctatgt cagacatcct aattttgata atatgtatcg tcaatatact tctgaacagc
    23581 tggaagaatt gattgcttct ttaaaagaga cgattcggtt cttagaatac caaaaatccc
    23641 tttctgttgc ttttcatgaa gggcagactc aagaagtgca acatcttcta gaaaacaaaa
    23701 tagaaggtaa cttatcctat cttcgaaaag atgaggggtg gttgctaaga attttgaaga
    23761 gataacttga gagttgatag tgtcaattct tttcttttta gcatagtcaa ttctatgatt
    23821 gccatctaga acaaagttga aaaagaaatc agagttacaa actattactg gattcgagct
    23881 aacagttttt aaagatttat caatagcata cccaattagt tgagaattag tcattagaga
    23941 tagtctattt tgagaattgt tttcaataaa ctgttgggta gatattgata tagttggaat
    24001 attaacctta tctat
//
LOCUS       DATRUK010000028        23446 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.028, whole genome shotgun sequence.
ACCESSION   DATRUK010000028 DATRUK010000000
VERSION     DATRUK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23446
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.028"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            714..2132
                     /locus_tag="VBE34_001067"
     CDS             714..2132
                     /locus_tag="VBE34_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HEO7263793.1"
                     /translation="MMTKETLAHYQKKIEQESEKKQSLDEHSWHVACFSRQEASIIGQ
                     GDVLFLIGLYHDLGKADRAFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKSRGVDKG
                     ERMTFNEMVGYVISAHHGMYDFCYCSDDAEYYSFNKFKNRINRDLDDYHYHEDIKGYA
                     IKLEEKLCDYGYKDLRELIDKAFDNYQQAMSSLNWQDNSEWDYYQSCMVRLYLSLLKN
                     ADILDTVNAYGLKISPMDKTERSSLKHSYLAAIEQKYASFGRPNNQLNTIRTEIAERV
                     KERGKRDSKGIYRLDLPTGAGKTNLSMRYAFHQLVHQDKSRFFYITPFLSVLEQNASE
                     IRKVTGDLGVLEHHSNMVKQANEDDDKDSLLSAYLIDSWDSQVVLTSMVQFFQTLFKT
                     KSANLRRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFFNKVMDTTIVLCTATQPAYD
                     SSEIDHRICYGGNLGELAEIVD"
     gene            2403..3362
                     /locus_tag="VBE34_001068"
     CDS             2403..3362
                     /locus_tag="VBE34_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7263794.1"
                     /translation="MLKVRYGVVSTAKVAPRFIEGVRLAGNGEVVAVSSRTLESAQAF
                     ANKYHLPKAYDKLEDMLADESIDVIYVATINQDHYKVAKAALLAGKHVLVEKPFTLTY
                     DQANELFTLAESCNLFLMEAQKSVFIPMTQVIKKLLASGEIGEVISISSTTAYPNIDH
                     VTWFRELELGGGTVHFMAPYALSYLQYLFDATITHASGTATFPKGQSDSQSKLLLQLS
                     NGVLVDIFLTTRLNLPHEMIIYGTEGRLIIPHFWKTTHAKLVRNDTSARTIQVDMVSD
                     FEKEAYHVSQMILEGQRVSHIMTPQLTLSGVKIIEGLYRSWGK"
     gene            3523..4425
                     /locus_tag="VBE34_001069"
     CDS             3523..4425
                     /locus_tag="VBE34_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO7263795.1"
                     /translation="MFSSDFGFEIINVDDLSLQEEKFLPSELLAYARDENESSFVRDI
                     EGHLTLVYQLLDTQGHVDDVRHVPRVIPVTLFLKEDGLFVLANHKNIILVKKALNRVE
                     KVDSPKHLLLSLVTAFSKQYFDVLDTISEERDKLINDLRKRPNKSNLARLANLQSGTV
                     HLMMGTKQNFEMLTDLQNIEQDKENTRNEKMQLQDAIIEARQLSNMCSLNSQVFQELS
                     SSYNNVLSNNLNDNVTTLTIISIGISIIAMVTSFYGMNVKLPFDSVDAVWVLIILITT
                     IITIMLSIVMYIYVHKEDNHPKHK"
     gene            4585..5649
                     /locus_tag="VBE34_001070"
     CDS             4585..5649
                     /locus_tag="VBE34_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEO7263796.1"
                     /translation="MSIESDFFRKKRFIFSSLEEFGFIKSDQEYIYCQTFMDNDFKAI
                     ITISLDGKIAGKVIDSALEEEYLPLRAANYNGSFVGEVRSAYMAILGDISDSCCKDLL
                     FTKDQSNRLAEKIATTFEDSVDYPFAKHPQYASYRVSGKWYALLFPLKMGKLENVPAQ
                     LSEEEVEVLNIKVNPQDMEILLQKEGIYPSYHMSKKTWVSIVLDNTLSDIEIFKLVSD
                     SRKLVSPNKKSNSEPEFWIIPANPKFYDIDKEFAEHQLIDWTQKGKIAVGDYVFIYIT
                     APIRALRYVCQVIERDLKTSTYLGARDVKAYMRLKLIRQFEDSTYPVAFLAEHGVKTV
                     RGPRRMTKELRQVMAKTILE"
     gene            5764..6756
                     /locus_tag="VBE34_001071"
     CDS             5764..6756
                     /locus_tag="VBE34_001071"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEO7263797.1"
                     /translation="MKKSFIHQQQEISFVKNTFTQYLIDKLEIVEVQGPILSQVGDGM
                     QDNLSGIEHPVSVKVLNIPEAEFEVVHSLAKWKRHTLARFGFNEGEGLFVHMKALRPD
                     EDSLDPTHSVYVDQWDWEKVIPDGRRNLDYLKETVEKIYKAIRLTELAVEARFDIESI
                     LPKRITFIHTEELVEKYPDLSPKERENAIAKEYGAVFLIGIGGELADGKPHDGRAPDY
                     DDWTTPSENGFKGLNGDILVWNEQLGTAFELSSMGIRVDEDALKRQVVLTGDEDRLEF
                     EWHKTLLRGFFPLTIGGGIGQSRLAMFLLRKKHIGEVQSSVWPKEVKDTFENIL"
     gene            complement(6801..7250)
                     /locus_tag="VBE34_001072"
     CDS             complement(6801..7250)
                     /locus_tag="VBE34_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001893380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transport accessory protein"
                     /protein_id="HEO7263798.1"
                     /translation="MIKKLLLTLIAILCLTTLKSNLSSADTMTISQQQYQHMITNSDL
                     IAITDKALVSKSQQSEATIFIGRPTCQYCRAFLPKLLKSQATLHSKIYYLDSQKYKGK
                     RLKSFFKKHHITTVPNLAHYQRGKMTKYLVQGSQATPQQIQTFLSQK"
     gene            7216..7920
                     /gene="rsmD"
                     /locus_tag="VBE34_001073"
     CDS             7216..7920
                     /gene="rsmD"
                     /locus_tag="VBE34_001073"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEO7263799.1"
                     /translation="MAIKVNNNFLIIMGTSFLLLLAYHIKKEGKEILSSLFVISNFFD
                     NSTLFFVIIVVMRVVAGTFGGRPLKTLDGKTTRPTTDKVKGAIFNMIGPFFEGGRVLD
                     LFSGSGSLAIEAISRGMDQAVLVEKDRRAQVVIQENIAMTKSPEQFQLLKIEANRALE
                     QLTGQFDLVLLDPPYAKEEIVKQIQIMDSKGLLGDDIMIVCETDKSVDLPEEIASFGI
                     WKQKIYGISKVTVYVR"
     gene            7932..8222
                     /locus_tag="VBE34_001074"
     CDS             7932..8222
                     /locus_tag="VBE34_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263800.1"
                     /translation="MDYKLVMIILILVILIYLLLRLHYLEELLKHYHFKNDEGVCSLS
                     RTKNPLYLLMAMITFLPNLINWIANGFEAIDIVRLIPVVVFLWLYYCGDDDE"
     gene            8219..8704
                     /gene="coaD"
                     /locus_tag="VBE34_001075"
     CDS             8219..8704
                     /gene="coaD"
                     /locus_tag="VBE34_001075"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEO7263801.1"
                     /translation="MTKKALFTGSFDPVTNGHLDIIERASYLFDHVYIGLFHNLEKQG
                     YFSIECRKKMLEEAIRQFKNVSVLVAQDRLAVDLAREVGAKYFVRGLRNSQDFDYEAN
                     LEFFNKQLADGIETVYLSTSPSLSPISSSRIRELIHFKASVKPFVPKSVFREVEKMSE
                     E"
     gene            8694..9767
                     /locus_tag="VBE34_001076"
     CDS             8694..9767
                     /locus_tag="VBE34_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEO7263802.1"
                     /translation="MKNKDPKRKHKSLLGLLKWWIIGFAFLLLVLASLVVRLPYYLEM
                     PGGAYDIRSVLKVNKKADKAKGSYNFVAVSVSQATPAQVLYAWLTPFTELSSKEETTG
                     GFSNDDYLRINQFYMETSQNESIYQALKLANKQVSLTYKGVYVLNLAKNSTFKDRLHL
                     ADTVTGVNGKSFKNSSQLIKYVAALHLGDKVKVQYTSQGKKKESVGKVIKLSNGKNGI
                     GIGLTDHTEVSSDVPVDFNTEGVGGPSAGLMFTLAIYDQLVKEDLRKGRKIAGTGTIE
                     QNGHVGDIGGAGLKVVSAAKKGMDIFFVPNNPIDKNAKKGKTKVQTNYQEAKAAAKRL
                     GTKMKIVPVQNVQQAIDYLKKTK"
     gene            9842..11176
                     /locus_tag="VBE34_001077"
     CDS             9842..11176
                     /locus_tag="VBE34_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO7263803.1"
                     /translation="MTELIRILHLNDLHSHFENFPKVKRFFHNNQAQPIETISLDLGD
                     NIDKSHPLTEASSGKANVQLMNELGIELATIGNNEGVGLSKKDLDQVYKDSDFTVIVG
                     NLKDNIIEPSWAKPYIIYETQQGTKLAFLAYTFPYYKTYEPNGWTIEDPIDCLKCHLQ
                     INEIKEANCRILMSHLGIRFDTRIAQEFSEIDLIIGAHTHHLFEEGELITGTYLAAAG
                     KYGRFVGSIDITFDNHTLKDILISTYDTKQLTGYPSDSDWAKEIESEGKEQLRKESLI
                     SFPAPLSLEESCQLVMDAMLFYAGADVAIINSGLIVQPFEKDFSRKNLHESLPHQMRL
                     AKLTVSSQELLEIYETIYQQGQFLAQQKIHGMGFRGKYFGEVLHSGFDYKNGKIVYNE
                     KDIDAKEEVILVIVDQYYFASYFECLKTKKVDLLFPELLRDVVADYLINMTL"
     gene            11245..11823
                     /locus_tag="VBE34_001078"
     CDS             11245..11823
                     /locus_tag="VBE34_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEO7263804.1"
                     /translation="MRKEVTPEMLNYNKYPGPQFIHFENIVKSDDIEFQLVINEKSAF
                     DVTVFGQRFSEILLKYDFIVGDWGNEQLRLRGFYKDASTIRKNSRISRLEDYIKEYCN
                     FGCAYFVLENPNPRDIKFDDERPHKRRKSRSKSQSSKSQTRNNRSQSNANAHFTSKKR
                     KDTKRRQERHIKEEQDKEMTSAKQHFVIRKKK"
     gene            11874..12980
                     /gene="rlmN"
                     /locus_tag="VBE34_001079"
     CDS             11874..12980
                     /gene="rlmN"
                     /locus_tag="VBE34_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEO7263805.1"
                     /translation="MPKKTDTPDYKPSIYSLTRDELIAWAIEHGEKKFRASQIWDWLY
                     KKRVQSFDEMTNISKDFIALLNENFVVNPLKQRIVQESADGTVKYLFELPDGMLIETV
                     LMRQYYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLNNGEITAQIMLVQKYFDERGQG
                     ERVSHIVVMGIGEPFDNYTNVLKFLRTVNDDNGLAIGARHITVSTSGLAHKIREFANE
                     GVQVNLAVSLHAPNNELRSSIMRINRSFPLEKLFAAIEYYIETTNRRVTFEYIMLNGV
                     NDTPENAQELADLTKKIRKLSYVNLIPYNPVSEHDQYSRSPKERVEAFYDVLKKNGVN
                     CVVRQEHGTDIDAACGQLRSNTMKRDRQKAKVGR"
     gene            12980..13495
                     /locus_tag="VBE34_001080"
     CDS             12980..13495
                     /locus_tag="VBE34_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEO7263806.1"
                     /translation="MLKRLFTEDGELTKISRRFVWMLVVIYCLIIVRMCFGPQIMIEG
                     VSTPNVQRFGRIVALLVPFNSFRSLDQLTSFKEIFWVIGQNVVNILLLFPLIIGLLSL
                     KPSLRKYKSVILLAFLMSLFIECTQVVLDILIDANRVFEIDDLWTNTLGGPFALWSYR
                     NIKGWLLTIRK"
     gene            13691..15436
                     /locus_tag="VBE34_001081"
     CDS             13691..15436
                     /locus_tag="VBE34_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7263807.1"
                     /translation="MSIIKNLWWFFKEEKKRYLIGILSLSLVAVLNLIPPKIMGSVID
                     AITTGKLTRPQLLWNLLGLVLSALAMYGLRYIWRMYILGTSYKLGQVVRYRLFEHFTK
                     MSPSFYQKYRTGDLMAHATNDINSLTRLAGGGVMSAVDASITALVTLITMFFTISWQM
                     TLIAVIPLPLMALATSKLGRKTHETFKESQAAFSELNNKVQESVSGVKVTKSFGYQEQ
                     EIASFQEINQMTFVKNMRTMTYDVMFDPLVLLFIGASYVLTLAMGAFMISKGQVTVGD
                     LVTFVTYLDMLVWPLMAIGFLFNMVQRGSVSYNRINSLLEQESDITDPLNPIKPVVNG
                     TLRYDIDFFRYDNEETLADIHFTLEKGQTLGLVGQTGSGKTSLIKLLLREHDVTQGKI
                     TLNKHDIRDYRLSELRQLIGYVPQDQFLFATSILENVRFGNPTLSINAVKEATKLAHV
                     YDDIEQMPAGFETLIGEKGVSLSGGQKQRIAMSRAMILDPDILILDDSLSAVDAKTEH
                     AIVENLKTNRQGKSTIISAHRLSAVVHADLILVMRDGRVIERGQHQELLNKGGWYAET
                     YASQQLEMEEAFDEV"
     gene            15426..17165
                     /locus_tag="VBE34_001082"
     CDS             15426..17165
                     /locus_tag="VBE34_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7263808.1"
                     /translation="MKSNQWQVFKRLISYLRPYKWFTVLALSLLLLTTVVKNIIPLIA
                     SHFIDHYLTNVNQTAVLILVGYYSMYVLQTLIQYFGNLFFARVSYSIVRDIRRDAFAN
                     MERLGMSYFDRTPAGSIVSRITNDTEAISDMFSGILSSFISAIFIFTVTLYTMLMLDI
                     KLTGLVALLLPVIFILVNVYRKKSVTVIAKTRSLLSDINSKLSESIEGIRIVQAFGQE
                     ERLKTEFEEINKEHVVYANRSMALDSLFLRPAMSLLKLLAYAVLMSYFGFTGVKGGLT
                     AGLMYAFIQYVNRLFDPLIEVTQNFSTLQTSMVSAGRVFDLIDETGFEPSQKNTEAFV
                     REGNIEFKNVSFSYDGKKQILDNVSFSVKKGETIAFVGATGSGKSSIINVFMRFYEFQ
                     SGQVLLDGKDIRDYSQEQLRKNIGLVLQDPFLYHGTIKSNIKMYQDITDQEVQDAAEF
                     VDADQFIQKLPDKYDSAVSERGSSFSTGQRQLLAFARTVASKPKILILDEATANIDSE
                     TEQIVQDSLAKMRQGRTTIAIAHRLSTIQDANCIYVLDRGKIIESGNHESLLDQKGTY
                     YRMYQLQAGMMEV"
     gene            17293..17859
                     /locus_tag="VBE34_001083"
     CDS             17293..17859
                     /locus_tag="VBE34_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEO7263809.1"
                     /translation="MLLLVDNYDSFTYNLKQYLSVYKEIFVIKNDDPNLFLLAESAEA
                     IVLSPGPGHPKDAGKILELINQFIGQKPILGICLGHQALAECLGGRLNLANHVMHGKQ
                     SWVTINDHTSLFKGIDSPTQVMRYHSLVVTDLPENIAIIARSNEDNEIMAFHCPSLKV
                     YAMQFHPESIGSIDGMKMIENFLTLIND"
     gene            complement(17927..18465)
                     /locus_tag="VBE34_001084"
                     /pseudo
     CDS             complement(17927..18465)
                     /locus_tag="VBE34_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857893.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
     gene            complement(18466..19458)
                     /gene="bioB"
                     /locus_tag="VBE34_001085"
     CDS             complement(18466..19458)
                     /gene="bioB"
                     /locus_tag="VBE34_001085"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEO7263810.1"
                     /translation="MSFQTNYIHLADEILSGKTSISYEQALEILNSDENWWEIYAAAL
                     YLKNQVSRNNIRLNVLLSAKQGLCAENCGYCSQSKESTADIDKFGLLPQNVILKQAIV
                     AHQNGASVFCIAMSGTKPSKREIEQLCQVIPEIKKSLPLEICLTAGFLDREQLHQLKQ
                     AGIDRINHNLNTPEENYPNIATTHSFKDRCDTLERIHNEDIDVCSGFICGMGESDEGL
                     ITLAFRLKELNPYSIPVNFLLAVEGTPLGKYNYLTPIKCLKIMAMLRFVFPFKELRLS
                     AGREVHFENFESLVTLLVDSTFLGNYLTEGGRNQHTDIEFLEKLQLNHTKKELI"
     gene            19583..20098
                     /locus_tag="VBE34_001086"
     CDS             19583..20098
                     /locus_tag="VBE34_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263811.1"
                     /translation="MKKLFKKAIAMCRDLPLNFVIVELSLYQREDGSSDNIKWISKRI
                     LEVTVTDQQWIYHLRMLNLPKLKPMSLASSDCPAREEKASLISIEECQKFTSLVRDKN
                     PFHQGEAAIVPAALLLEKIMEVRNGKIKEIHLTFLSPVRVNQEFSVQVVDNTVIIFQH
                     QILCVRGTYEE"
     gene            20088..21203
                     /locus_tag="VBE34_001087"
     CDS             20088..21203
                     /locus_tag="VBE34_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEO7263812.1"
                     /translation="MKNRDVYIGFGLRTPIGIKGKQFKHYRPELLGAHLLNQIKKIES
                     ESNIDSIICGNTVGTGGNIGRLMTLFSDYESYIPVQTIDMQCASSSSALFFGYLKISA
                     GINEKVLVGGIESSSLQPMRRYAKEDNRNGEYTVAQFSPDSYAETVMLEGAQRVCQKY
                     GFRREMLDKLAFLSHKRALTAKQGGYLEEVILPMEGMRDQGVRKLKEAFFQKLPRLME
                     NSPLLTIGNVCLMHDAAAFLTLQSQKTEFRIVHIVEVAGDPKLSPELVHTATEKLLTE
                     THTKISDYDAIEWNEPFAAIDALFNHYYPEEREKFNIFGGALAYGHPYACSGIINILH
                     LMQALKYKNKPMGLTAIAGAGGVGMAISIEYLGVKNA"
     gene            21196..22425
                     /locus_tag="VBE34_001088"
     CDS             21196..22425
                     /locus_tag="VBE34_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEO7263813.1"
                     /translation="MLESLKTIVKTNSDKKLFDGDLQVSYGEFYNLVRQDMASQDNRK
                     HVISTHSLLNQLVRFVSKLCQKALPIICKPNLTHNEISRLEKEVQYAPQLADFGVLSS
                     GTTADAKLLWRSFASWSDFFSIQNAYFSVTSNSRLFIQGDFSFTGNLNLALSLLLLGG
                     TLVVTQKNSVKYWQTLWEKTGVTHLYLLPSYLKLVEQYSKETALDNKTIITSSQYVSD
                     SLLEGLYIKHPKVSVKIFYGASELNYVSWYDGRDIRDKPQYVGEIVPNVAVRIKEGRI
                     FVKTPYSICGLSSEYCAGDYGELIDGKLYLFGRGDDWCNQSGIKLYLPRLIEKIKTCP
                     YIKDAVAFTKESQSHGQESHCCIVLIENQMQQECLKWLSEHFEKKYGFKHYHIVSKIP
                     LMPSGKIDYQQLKRQLA"
     gene            22538..23170
                     /gene="nth"
                     /locus_tag="VBE34_001089"
     CDS             22538..23170
                     /gene="nth"
                     /locus_tag="VBE34_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEO7263814.1"
                     /translation="MLSKAKSRYIIREIIKLFPDAKPSLDFTNVFELLVAVMLSAQTT
                     DAAVNKVTPALFERFPNPLVLAQADPKEIEPYISKIGLYRNKARFLNQCAKQLIEHFD
                     GKVPQTRQELESLSGVGRKTANVVMSVGFGIPAFAVDTHVTRICKHHQICKQSASPLE
                     IEKRVMEVLPPEEWLAAHQSMIYFGRAICHPKNPKCDQYPQLYHFPDNLK"
     gene            complement(23171..>23446)
                     /locus_tag="VBE34_001090"
     CDS             complement(23171..>23446)
                     /locus_tag="VBE34_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001884316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEO7263815.1"
                     /translation="ILTPITKVSIVLCLFGILAEVVDINIGSGDFLYLATIGLSLPLH
                     QMLFIIQIGAWLGIIYCLVMRKMKKTIAFLPFLSIAYIIVTSYSLLF"
BASE COUNT         7609 a         3614 c         4688 g         7535 t
ORIGIN      
        1 atgaatagac atagaaagtg aagatacatg aggactttat aaatagcagg aaagtagata
       61 ctagttagat atagtagtgc aagatagatg agaaaaaata acgaatactt taacttggtc
      121 ataacaattc cattctaatg gtagtaagca tgcataaaat gtaggtttta tcatactact
      181 tgaatccagc aactagatag agggggttac atacgataag acagtagtat tttaaaccga
      241 tcggttgact tattgatact gtttagggaa caagtgtttt gttgtcaatg ttaaatttaa
      301 tttagcattt tgatggtatc accgcaagaa tgaaaggaaa agttgtatgt gctgaaaaaa
      361 ctaatgcttg gcgatttaga acacacgttt actagtcgag atgacaaaaa ataagtacca
      421 aatgtaaagg tcgatattat cagcattgtc agcgttaggt gttataaaaa tttaacatct
      481 tccaattaag ctaggtttaa gtttcctaag ttgaactacg cctagccgaa aaataatttc
      541 ttttttacag aactcctaac aaggattttg aattgtcaag attcttgttt ttttggaaag
      601 tcatttagag gctttttggg ggaagtatta actattgtat ccgtttacaa ttcctttcaa
      661 aaatgttagt atatcattga gggatatgag ttatggataa gggagagagg atgatgatga
      721 ctaaggaaac gttggcacat tatcaaaaga aaatagaaca agaatctgaa aaaaagcagt
      781 ctttagatga gcactcatgg catgtggcct gttttagtcg acaggaagca tctataattg
      841 gtcaaggaga tgtgcttttt ttaattggtc tataccacga tctaggcaaa gctgatcgag
      901 cctttcaaga taaattatta aataatccaa atcgacatgt tgatcattct tatgcagggg
      961 caaaatactt atgctctatt attgggcctc atctaaaaag tcgaggggtt gataaaggtg
     1021 agagaatgac attcaatgaa atggtgggtt atgtcatctc ggctcatcat gggatgtatg
     1081 atttttgcta ctgttctgac gatgctgaat attatagctt taataaattt aaaaaccgta
     1141 tcaacagaga cttagatgat tatcactatc acgaagatat taaagggtat gctataaaat
     1201 tagaagaaaa attgtgtgat tacggctaca aagatttaag ggagcttatt gataaagctt
     1261 ttgataatta ccaacaagct atgtcttcct taaactggca agataatagt gagtgggatt
     1321 attatcagtc ttgtatggtg agactttact tgtcgctctt aaaaaacgct gatattttgg
     1381 acacagtaaa tgcctatggt cttaaaataa gtcctatgga taaaacagag cgatcctctc
     1441 taaaacattc ttatttagca gccattgaac aaaaatatgc tagctttgga cgaccaaaca
     1501 atcagttgaa tactattcgg acagaaattg ctgagcgtgt taaagaaaga ggtaaacggg
     1561 attccaaagg gatctatcgc ttagatttac cgacaggagc tggcaagact aatcttagta
     1621 tgcgctatgc gtttcaccaa ttagttcatc aagacaaatc aagatttttt tacataacgc
     1681 cctttctttc ggttcttgag caaaatgctt ccgaaattag aaaagttacg ggtgaccttg
     1741 gcgttctaga acaccattcc aatatggtta aacaggctaa tgaagatgat gacaaggaca
     1801 gtttattgtc agcttatctt attgatagct gggatagtca agtagtcttg acttctatgg
     1861 ttcaattttt ccaaacgctt tttaaaacaa aatcggctaa tttgagacgt ttttcaagtt
     1921 tgattaatag tgttgtgatt ctagatgaag ttcagtcact gcctattgaa gtcaccactt
     1981 tgtttaattt aacgatgaat ttttttaata aagttatgga tacaaccatc gttctttgta
     2041 cagcaacaca acctgcttat gattcttcag agattgacca tcgtatctgt tatggaggga
     2101 atttgggaga attagctgaa atagtggatt gaaataagtt tatacgttgc ttcatcacca
     2161 agttttacgt cgcacccatt gcgggtgcgt ggattgaaat ataagttcag caacttcacg
     2221 agaaaatgtt cctcgtcgca ccctttgcgg gtgtgtagtt tcaactattt taaatctttc
     2281 aaattatgct attaaaattg tctaactttt aacagagatg cgattgattt gtattaatcc
     2341 ctaaaaagat aaattgctag ttcttatgct ataataactc tatcaatgtc aaaggagttt
     2401 ttatgttaaa ggtaagatat ggtgttgtgt caacggcaaa ggtggcgcct cgttttattg
     2461 aaggagtccg cttagcagga aatggtgaag ttgtggctgt atcgagtcga actcttgagt
     2521 ctgcgcaagc ttttgccaac aaatatcatc tcccaaaggc ttacgataag ttagaggata
     2581 tgttagcaga tgagtcgatt gatgtgattt atgtggcaac aattaatcaa gaccattata
     2641 aagttgctaa agcagctctg ttagcgggaa aacatgtgtt ggtagaaaag cctttcacat
     2701 tgacttacga ccaagcaaat gaattattca ctttggcaga aagttgtaat ctgtttttga
     2761 tggaagcaca aaaatcggta tttattccga tgacacaagt gattaaaaaa ttacttgcct
     2821 ctggtgaaat aggagaagtg atctccatct catctacaac agcctaccca aacattgacc
     2881 atgtaacatg gtttagagaa cttgagttag gtggaggaac agtgcatttt atggcgccct
     2941 atgccctgtc ttatttacaa tacctatttg atgcgactat aactcatgct agtgggactg
     3001 cgacttttcc aaaaggtcaa agcgatagtc aatccaaact actcttgcaa ttatcaaatg
     3061 gggtcctcgt agatatcttc ttgacaacac gtttaaattt accgcatgaa atgattattt
     3121 atggaactga aggtcgtcta atcattccac atttttggaa aaccactcat gcaaaattgg
     3181 tgagaaatga tactagtgcg cgaacgattc aagttgacat ggttagtgat tttgagaaag
     3241 aagcttatca tgttagtcaa atgattttag aagggcaaag ggtaagtcat atcatgacgc
     3301 ctcagttgac gctgtcaggt gttaagatta tagaaggctt ataccgttca tgggggaaat
     3361 gatgctggct atctagcata ttaataagga gtactagatg ttattttagt agtccttttt
     3421 tgttttgctc tcataaggtt aattagattt aaaagggttt tctacttgtt ttttctcgat
     3481 ttttgtttca taatgtagac aagtttagag tgaggtgctt ctatgttttc gagcgatttt
     3541 ggatttgaga taattaatgt tgacgatcta tccttacaag aggagaaatt tttaccttca
     3601 gaattgctgg cttatgcacg tgatgaaaac gagtcttctt tcgtacgtga tattgagggg
     3661 catttgacac ttgtttacca gcttttggat acgcaaggtc acgttgatga tgttcgtcat
     3721 gtgccgagag tgataccagt gactctattt ttgaaagaag atggcttgtt tgtattagct
     3781 aatcataaaa atattatcct tgtaaaaaaa gcacttaatc gcgttgaaaa ggtagattcg
     3841 ccgaaacacc tcttattatc attagtgact gctttctcaa agcaatattt tgatgtttta
     3901 gatactattt ctgaagaaag agataaacta atcaatgatt tgcgtaaaag accaaacaaa
     3961 tcgaacttgg caaggctggc aaatttacaa agtggaacag tacacttaat gatgggaact
     4021 aagcaaaatt ttgaaatgct gaccgattta caaaacattg agcaagataa agagaatact
     4081 agaaatgaaa agatgcaact tcaagatgct attattgaag caagacaatt atctaacatg
     4141 tgttccttaa attcacaggt tttccaagaa ctttcttctt cttataataa tgtcctcagc
     4201 aataacctta acgacaatgt aactacctta acaattattt ccattggtat ttccattatt
     4261 gctatggtta ccagctttta tggaatgaat gttaagttgc cttttgatag tgttgatgct
     4321 gtctgggtat tgattatttt aattacgact atcattacta ttatgctctc tattgttatg
     4381 tacatttatg tccataaaga ggataatcat cccaaacata aatagagaat taataagagg
     4441 gtgctatcaa ttagtttagt tttaagcatg gcaacatgct ttttttgtac aaagaaacta
     4501 gaattcatct ttgactttga gcttgctttc taactcttaa aacgacataa tagaggataa
     4561 tgtacagtga atagagaggt tactatgtca atagaatcag attttttccg gaaaaaacgt
     4621 tttatttttt catcgcttga ggagtttgga tttatcaaat ctgaccaaga gtatatctat
     4681 tgtcaaacat ttatggataa tgactttaaa gctattatca cgatttcatt ggatggaaag
     4741 atagctggta aggttattga ttcggcactt gaagaagaat atttaccgct tcgagcagca
     4801 aattataatg gttcgtttgt tggcgaagta cgatcggctt atatggctat attaggtgat
     4861 atttcagatt cttgttgtaa ggatttatta tttaccaaag atcaaagtaa tcgtttagcg
     4921 gagaagatag ctacaacttt tgaagattca gttgattatc catttgcgaa gcacccacaa
     4981 tatgcttctt atcgtgtatc aggtaaatgg tatgctcttc tttttccatt gaagatgggg
     5041 aagctagaaa atgttccagc tcaactgtca gaagaagagg ttgaagtttt gaatattaaa
     5101 gtcaatcctc aagatatgga aatactctta caaaaagaag gtatttatcc ttcttatcat
     5161 atgtctaaaa agacttgggt atctatcgtt ttagacaata ctttgtctga tatagaaatt
     5221 tttaaattgg tatcagatag tcgtaagctt gtttctccca ataaaaaatc aaattctgag
     5281 ccagagtttt ggattattcc tgctaatcct aaattttacg atattgataa ggagtttgca
     5341 gagcatcaat tgattgattg gactcaaaaa ggaaagattg cggtgggaga ttatgtgttt
     5401 atttatatta cagcacctat tcgtgctttg cgctatgtct gtcaagtgat agaacgtgac
     5461 cttaaaacaa gtacttactt aggagcaaga gatgtcaaag cttatatgcg actcaaactg
     5521 ataaggcaat ttgaagattc tacctatcct gtagcatttt tagcagaaca cggcgttaag
     5581 actgtgagag gaccacggcg aatgaccaag gaattgcgac aagtcatggc aaaaacaatt
     5641 ctagagtaat gactttcaga aaaaaacttg taaaaaagtc agaataattc tacaattcct
     5701 cttccatttt gttacttgaa ggagtataat agctctaaca ttttataatt taaggaggaa
     5761 catatgaaga aaagctttat tcatcagcaa caggagattt cttttgtcaa aaatactttt
     5821 acacaatatt tgattgataa gttagaaatc gtcgaagtgc aaggacctat tcttagtcag
     5881 gtcggggatg gtatgcaaga taatttatct ggtattgaac atcctgtatc ggtgaaagtc
     5941 cttaatatac ctgaagctga atttgaagtc gttcactcat tggctaaatg gaaacgtcat
     6001 actttggcaa gatttgggtt taatgaggga gaaggtcttt tcgttcatat gaaagctctt
     6061 cgtccagatg aagattctct tgatccaaca cactcagttt acgtagatca gtgggactgg
     6121 gagaaggtca ttcctgatgg tagacgtaat ttggactacc taaaagaaac tgtggaaaaa
     6181 atctacaaag ctatccgcct aacagagctt gctgtggaag ctcgttttga tattgaatcc
     6241 attttaccaa agcgtattac ctttattcat acagaagaat tggtagaaaa atacccagac
     6301 ttaagcccta aagagcgtga aaacgccatc gctaaggagt atggcgcagt ctttttgatt
     6361 ggtattggtg gagagttagc agatggtaaa ccacatgatg gtcgtgcacc ggactatgat
     6421 gactggacaa caccatcaga aaatggtttt aaaggtctca atggtgatat tttagtatgg
     6481 aacgaacagc ttggaactgc ctttgaacta tcatcaatgg gaattcgagt tgatgaagat
     6541 gctcttaaac gtcaagttgt tctcaccggt gatgaagatc gtttagagtt tgagtggcat
     6601 aagacgctat tacgtggttt cttccctctg actattggtg ggggaattgg tcaatcacga
     6661 ttagctatgt tcttgcttcg taaaaaacat attggagaag tacagtcaag tgtctggcca
     6721 aaagaagtca aagatacctt cgaaaatata ttataaaaaa tcagccaagc agttttgttt
     6781 ggctgatttt aacaataagt ttatttttgg cttaagaaag tttggatttg ttgaggtgta
     6841 gcttggcttc cttgaacaag atatttagtc atttttcctc gttgataatg tgctaaattt
     6901 ggaacagtgg taatatgatg ctttttgaaa aaactcttca atcgtttccc tttgtatttt
     6961 tgactatcaa ggtagtaaat ttttgaatga agtgtggctt gagattttaa taattttggt
     7021 aagaaagctc tacaatattg gcaagttgga cgtcctataa aaatagtcgc ttcagattgt
     7081 tgacttttgc ttactaaggc cttatctgtg atggcgatta agtcactatt tgttatcatg
     7141 tgttggtatt gttgttgaga aatagtcatg gtgtcagcgc tacttaagtt tgattttagc
     7201 gttgtaaggc ataatatggc tattaaagtt aataataatt ttttaatcat tatgggaacc
     7261 tcgtttctct tactgctagc ttatcatatc aaaaaagaag gaaaagagat actatcttct
     7321 ctgtttgtta taagcaattt ttttgataac tctaccttat tttttgttat aatagtggtt
     7381 atgagagtag tagcaggaac atttggaggg cgtcctttaa aaacgcttga tggaaaaaca
     7441 actcgtccaa caactgataa ggttaaaggc gctattttta atatgattgg tccttttttt
     7501 gaaggtggtc gtgttttaga ccttttttct ggcagtggta gcttagctat tgaggcgatc
     7561 tcaaggggaa tggaccaagc tgtcctagtt gaaaaagata ggcgtgcgca ggtcgttatt
     7621 caagaaaata ttgcaatgac taagagtccg gagcaatttc aattattaaa aatagaagcc
     7681 aaccgtgctt tagaacaatt aacgggacaa tttgatttgg tcttgttgga tccgccatat
     7741 gctaaggaag aaattgtaaa gcaaatccaa atcatggata gcaagggttt attaggcgat
     7801 gatatcatga ttgtttgtga aacggacaaa tcagttgatt tacctgaaga aattgcttct
     7861 tttgggatat ggaaacagaa aatttatggc atttctaaag taacggtcta cgttcgttag
     7921 gaggctttag tatggattac aagttagtga tgattattct catcttagtc atattgattt
     7981 atttgttgtt gcgccttcac tatttagaag agttgctaaa acattatcac ttcaaaaatg
     8041 atgaaggagt ttgttcatta tcaagaacaa aaaatccatt gtatctatta atggcaatga
     8101 ttactttttt gccaaatctt atcaattgga tagctaacgg ttttgaggca atagacattg
     8161 ttcgcctgat tcctgtggta gtctttttat ggttgtatta ttgtggagat gacgacgaat
     8221 gacaaagaaa gctttattta caggttcatt tgatcctgtt actaatggac atttagatat
     8281 tattgagcgc gccagctatc tatttgatca tgtttacatt gggttatttc ataaccttga
     8341 gaagcaaggt tacttttcga ttgagtgccg taaaaaaatg ttagaagaag ctatccgtca
     8401 gtttaagaat gtttcagttt tagtggcgca agatagacta gctgttgact tagcaaggga
     8461 agtgggagca aaatattttg ttcgaggtct gcgtaatagc caggattttg attatgaagc
     8521 caatttggaa ttttttaata aacagttggc agatggtatt gagacagttt atttatcaac
     8581 gtcgccatct ttgagtccga tatcatctag tcgcatccgt gaattgatac attttaaggc
     8641 ttctgtgaag ccttttgttc caaagagtgt ttttagagaa gtggagaaaa tgagtgaaga
     8701 ataaagatcc aaaaagaaaa cataaaagtt tgctaggtct gcttaaatgg tggattatag
     8761 gttttgcttt tttattgtta gtattagcaa gccttgtggt gaggctacct tattatttgg
     8821 aaatgccagg tggagcttat gatatccgtt cggtattgaa agtgaataaa aaggctgata
     8881 aagctaaggg ttcgtacaat tttgtagcgg tatctgtcag tcaagcaacg ccagctcagg
     8941 ttctttatgc ttggttaact ccttttacag aattatctag taaagaggag acaacaggtg
     9001 gctttagtaa tgacgattat ctcagaatta atcaattcta catggagact tctcaaaatg
     9061 agtccatcta tcaagcttta aagttagcta ataagcaagt atctcttact tataaaggag
     9121 tttatgtgct taacttagct aaaaattcga catttaaaga tagattacat ttagctgata
     9181 cagtcacagg agttaatggc aaaagtttca aaaattcttc tcaacttatc aaatatgttg
     9241 cagctcttca tctaggtgat aaagtaaagg tacaatacac aagtcaaggt aaaaagaaag
     9301 aatcagttgg taaagttatt aaactatcaa acggtaaaaa tggtattggt atcggtttga
     9361 cagaccatac agaagtatca tctgatgttc ctgtggactt taatactgaa ggtgttggag
     9421 gaccaagtgc aggtttaatg tttactttag cgatttacga ccaattagtt aaagaagatt
     9481 tacgtaaagg acgtaagatt gcaggcactg gtactattga gcaaaatggg catgttggcg
     9541 atattggtgg agcaggtttg aaagttgttt cagcggctaa aaaaggaatg gatattttct
     9601 ttgttcctaa taatccgatc gataaaaatg ctaaaaaagg caaaacaaag gttcagacga
     9661 attatcaaga ggcaaaagca gcagctaagc gattaggtac gaagatgaaa attgtaccag
     9721 ttcaaaatgt tcagcaagct attgattatt tgaaaaaaac aaaataaact caaagagttc
     9781 gggcgtctgc tcggcttttt tgttttgcga catttaacta tcaaagtgct aaaatgatag
     9841 tatgacagag cttataagaa tactacattt aaatgatctg cactctcatt ttgagaattt
     9901 tcctaaggta aaacgatttt ttcataacaa tcaagcgcag ccaatagaga cgatttcact
     9961 tgacttgggg gataatattg ataaaagcca tcctttgaca gaagcaagct cgggcaaggc
    10021 taatgtccaa ttgatgaatg aactgggtat tgagcttgct actattggta ataatgaagg
    10081 agttggttta tccaaaaaag acttggacca agtctacaaa gattcagatt ttacggttat
    10141 tgttggtaat ctaaaagata acataatcga accgagttgg gcaaaacctt acattattta
    10201 tgagacacag caaggaacta agcttgcttt tttagcttat acttttcctt attataaaac
    10261 ctatgaacct aatggatgga cgatagaaga tccaattgac tgtcttaaat gccatttaca
    10321 aataaatgag ataaaagagg caaattgtcg tattttgatg agtcatttag gtattcgttt
    10381 tgatacaagg attgctcaag aattttcaga aattgacctt attataggtg cacatacaca
    10441 ccatttattt gaggaagggg agctaattac cggtacatat cttgcagcag ctggtaaata
    10501 tggtcgcttt gttgggagta ttgatataac ttttgataat catacactaa aagatatttt
    10561 aatctcaaca tatgatacaa aacaactaac tggttatcct tcggacagtg attgggctaa
    10621 ggagattgag tcagaaggta aagaacagct tagaaaagaa agtttaatat cctttccagc
    10681 accattatca ttagaagaat cgtgtcaact agtcatggat gcgatgctgt tttacgcagg
    10741 tgcagatgtt gctattatca attctggctt aattgttcag ccatttgaaa aagacttttc
    10801 gaggaaaaat ctgcatgaat cattacctca tcaaatgcgt ctcgctaaat tgacagtgag
    10861 tagtcaagaa cttttagaga tttatgaaac tatttatcaa caaggtcaat ttttagcgca
    10921 acaaaagatt catggtatgg gctttcgtgg caagtacttt ggagaagttt tacattcggg
    10981 atttgattac aaaaatggaa aaatagtgta taatgagaaa gatattgatg ccaaagaaga
    11041 agtgattctt gtaatcgttg atcagtatta ttttgcatcg tattttgagt gtttaaaaac
    11101 aaaaaaagta gacttgcttt ttcctgaatt actcagagat gtggtagcag attatttaat
    11161 aaatatgaca ctataaatga aaaatagata aataaagagt agaagctagt cgtttctctc
    11221 aatgatttta gagaaaggag tattatgcga aaagaagtga caccagagat gcttaactat
    11281 aataagtatc ctggcccaca gtttattcac tttgaaaata tcgttaaaag tgatgatatt
    11341 gaatttcaac ttgttattaa tgaaaaatca gcttttgatg tgactgtctt tggacaacgt
    11401 ttttctgaga ttttattaaa atatgatttt atcgttggcg attggggtaa cgagcagttg
    11461 aggctaagag gcttttacaa agatgctagt acgattagaa aaaatagccg gatttcacgt
    11521 ttagaagatt atattaaaga gtattgtaac tttggttgtg cttattttgt gttggagaat
    11581 ccaaatccta gagatattaa atttgatgat gaaagacctc ataagcgtcg taagtcaaga
    11641 tccaaatcac aatcatcaaa gtcacaaact agaaataatc gttcccagtc aaatgccaat
    11701 gctcatttta caagtaaaaa gcgtaaagac acaaaacgcc gtcaagaacg tcatattaaa
    11761 gaagagcaag ataaggaaat gacctctgca aagcagcatt ttgttattcg taagaaaaaa
    11821 taaaaaaagg ataaactgta atcgtttatt ccaccatgaa gaaagaaaat attatgccta
    11881 aaaaaactga tacacccgac tataaaccct ctatttatag cttaactcgc gatgaactaa
    11941 tagcttgggc tattgaacat ggagaaaaga aattccgtgc ttcacaaatc tgggactggc
    12001 tctataaaaa gcgtgtgcaa agcttcgatg aaatgactaa catttccaaa gattttattg
    12061 cacttttgaa tgagaatttt gttgtcaatc ctctaaaaca acgtattgtt caagaatctg
    12121 ctgatggaac tgttaaatac ctttttgaat tacctgatgg catgctgatt gagacggttt
    12181 tgatgcgtca atattatggt ttatcagtat gtgtaacgac tcaagttggt tgtaacattg
    12241 gttgcacttt ctgtgcaagt ggtttaataa agaaacaacg tgatcttaac aatggcgaaa
    12301 tcactgctca aattatgctt gttcaaaaat attttgatga acgtggacaa ggtgagcgcg
    12361 ttagtcacat tgtggtcatg ggaataggtg aaccctttga taactatacc aatgtattaa
    12421 aattcttgcg tacagttaat gatgataatg gattagctat tggtgcacgt cacattacag
    12481 tgtctacttc aggtttagca cataaaattc gtgaatttgc aaatgaaggt gtacaagtta
    12541 acttagcagt atctcttcat gctccaaata atgaattacg atcaagtatc atgcgtatta
    12601 atcgttcttt ccctcttgaa aagttgtttg cggctattga atattatatt gagacgacta
    12661 accgtcgtgt aacatttgag tatattatgt taaatggtgt caacgatacc ccagaaaatg
    12721 ctcaagagtt agcagattta acgaaaaaga ttcgcaaatt atcttatgtc aatttaattc
    12781 cttacaatcc tgtctcagaa catgaccagt atagtcgtag tcctaaagag cgtgtggaag
    12841 ctttctatga cgttttgaag aaaaatggag ttaattgtgt tgttcgtcaa gaacatggta
    12901 cggacattga tgcagcttgc ggacaattac gttcaaacac aatgaaacgc gatcgtcaaa
    12961 aagcgaaggt agggcggtaa tgcttaaaag attatttact gaagatgggg aattgacaaa
    13021 gattagtcgt cgtttcgttt ggatgttagt ggttatctat tgtcttatta ttgtcaggat
    13081 gtgttttggg cctcaaatta tgattgaggg ggtatcaact ccgaatgttc agcgcttcgg
    13141 aagaattgta gctcttttag taccatttaa ttcttttcgt agtttagatc agctaactag
    13201 ctttaaagag attttttggg ttattggtca aaatgtagtg aatattttac tgctgtttcc
    13261 tctcattata gggttactat ccctaaagcc aagtttacgg aaatataaaa gcgttatatt
    13321 acttgctttc ttgatgtctc ttttcataga gtgtactcaa gttgttttag atattttaat
    13381 agatgctaat cgggtttttg aaatcgacga tctatggaca aataccttag gcggtccttt
    13441 cgccctatgg agttatcgaa acataaaagg ttggcttcta actattagaa aatgaaaata
    13501 atgtaaaggc tatgatattg ttcaagcctt ttactattta aaaaggatca ctttagttga
    13561 cgaaagtaag cttatagagg cataatagag agttgttaaa tgagaatttt aaaacacttt
    13621 ttatgtcttc tgattgattt ggtcttctga cttaattatg atgtttttca acctttgaga
    13681 aaggaaaaat atgtctatta taaaaaattt gtggtggttt tttaaggaag aaaagaagcg
    13741 ctatcttatt gggattttgt cattaagttt ggttgcagtg ttaaatctta tccctcctaa
    13801 aatcatggga tcagttattg atgctattac aactggaaaa ttaacaagac cacaattact
    13861 atggaattta ttaggtttgg ttttgtcagc tttagctatg tatgggctgc gttatatttg
    13921 gcgtatgtat attttaggga cttcttacaa attaggccaa gttgtcagat accgtttatt
    13981 tgaacatttt acaaaaatgt ctccttcttt ttatcagaaa tatcgtacag gtgatttaat
    14041 ggcgcacgcg accaacgaca tcaattctct aacacgtctt gcaggaggag gagttatgtc
    14101 agcagtggat gcctctatca cagcattagt aacgcttatc accatgttct ttactatttc
    14161 gtggcaaatg acattaattg cggttatccc tttgccctta atggccttag caactagtaa
    14221 attggggcga aaaacccatg aaaccttcaa agaatctcag gcagcctttt cagaattaaa
    14281 taataaagtg caagaaagtg tctctggcgt caaagtgact aaatcatttg gttatcaaga
    14341 acaagaaatt gcttcttttc aagagattaa tcagatgact ttcgtgaaga acatgcggac
    14401 catgacttat gatgtcatgt ttgatccttt agttcttctt tttataggtg cctcctacgt
    14461 attaacattg gctatgggag cttttatgat ttcaaaaggt caagttactg ttggtgactt
    14521 ggtaacattt gtgacgtatt tagatatgtt ggtatggccc ttgatggcga ttggtttctt
    14581 gttcaatatg gtacagcgtg gtagtgtttc ttataaccgt attaatagtc tacttgagca
    14641 agaatcggat ataactgatc ctttaaatcc tatcaaacct gttgtcaatg gaacattaag
    14701 atatgatatt gatttcttta gatacgacaa tgaggaaacc ttagccgata ttcatttcac
    14761 cttagaaaaa ggtcaaacct taggtttggt aggtcaaacg ggatcaggga agacaagtct
    14821 tattaagtta ttgctacgtg aacatgatgt gactcagggg aaaattactt taaataaaca
    14881 tgatatacgt gattatcgat tgtctgagtt acgtcaacta atcggttatg ttcctcaaga
    14941 tcagttttta tttgctacca gtattttaga aaatgttcgc tttggaaatc caactctatc
    15001 tatcaatgct gtcaaagaag caactaaatt ggcacatgtt tacgatgaca ttgaacagat
    15061 gccagcagga tttgagactc taattggaga aaaaggagtc tcattatctg gtggacaaaa
    15121 acaaaggatt gcgatgagtc gtgccatgat tttagatcca gatattctta ttttggatga
    15181 ttctctatca gcagtggacg ctaaaacgga acatgctatt gttgagaatc ttaaaacgaa
    15241 tcgtcaaggg aaatcgacta ttatttcagc acatcgttta tcagctgttg tgcacgcaga
    15301 ccttatctta gttatgcgag acggcagagt cattgagcga ggtcaacatc aagagttgct
    15361 aaataaaggt ggttggtatg ctgaaacgta tgcctcacag caattagaaa tggaggaagc
    15421 atttgatgaa gtctaatcaa tggcaagtct ttaagagatt aatctcctat ttacgccctt
    15481 ataaatggtt tacagtatta gctctatctc tcttattgtt gacgactgtt gttaaaaata
    15541 ttattccttt aattgcttca cattttattg atcactatct gacaaatgtt aatcaaacag
    15601 cagttcttat tttagtggga tattattcaa tgtatgtctt gcagacctta attcaatatt
    15661 ttgggaatct cttttttgcg cgtgtttctt atagtattgt tagagatatt cgtagagatg
    15721 cttttgctaa tatggaaagg ctaggcatgt cttattttga taggacaccg gcaggatcta
    15781 ttgtgtcacg tattactaat gatactgaag caatatctga tatgttttcg ggtattttat
    15841 caagttttat ctcggcgata tttattttta cagttactct gtacactatg ttgatgctag
    15901 acattaaact aacaggactc gtcgctcttt tgttacctgt tatctttata ttagtgaatg
    15961 tctatcggaa aaaatcagtc actgtcattg ctaaaacgag aagtttactt agtgatatca
    16021 acagtaaatt atcagaaagt attgaaggaa ttcgcattgt acaggctttt ggtcaagaag
    16081 agcgcttgaa gactgaattt gaggaaatta acaaagagca tgttgtgtat gccaatcgtt
    16141 ctatggctct tgatagtctc ttcttaagac cggcgatgtc tcttttaaaa ctcctagcat
    16201 atgctgttct tatgtcttat tttggattta caggagttaa aggaggtctt acggcaggat
    16261 taatgtatgc ttttattcag tacgttaatc gtctatttga ccctttaatt gaagtaacgc
    16321 aaaatttttc aaccttacaa acatcaatgg tatcagcagg gcgtgtgttt gatctgattg
    16381 atgaaacagg ttttgaacca agccaaaaaa atacagaagc ttttgtgaga gaaggaaata
    16441 tcgaatttaa aaatgtatca ttctcatatg atggaaaaaa acaaatcctt gataatgttt
    16501 cttttagtgt taaaaaaggt gaaactattg cttttgtagg agcgacaggt tctggaaaat
    16561 catctattat taatgtcttt atgaggtttt acgaatttca atctggacaa gttttattag
    16621 atgggaagga tattagggat tactcacaag aacaattgcg aaagaatatt ggactcgtct
    16681 tacaagaccc ctttttatat catggcacta ttaaatctaa tatcaaaatg taccaagaca
    16741 tcaccgatca agaggtacag gacgcagcag agtttgttga tgctgatcaa tttattcaga
    16801 agttgccaga taagtacgat tcagctgttt cagaacgtgg gtcaagtttt tcaactggac
    16861 aacgccaact attagcgttt gccagaactg tggctagtaa acctaaaatt ttaatcttag
    16921 atgaggcgac agcaaatatt gattcggaaa cagagcagat tgtacaagat tcgttagcaa
    16981 aaatgcgtca aggtcgaaca acaattgcca ttgctcaccg cttatcaacc attcaagatg
    17041 ctaattgtat ctatgttctt gatagaggaa agataattga aagtgggaat cacgagtctt
    17101 tattagacca aaaagggact tattatcgta tgtaccaatt acaagccggc atgatggagg
    17161 tctagaaaat ttaggctatt caaaaggcat aatctgtgag gcttagaggt tggaatagtt
    17221 attcctctaa gcctttttta tattttacaa ttagtgtata atgaggtcaa agtacaagga
    17281 aaggtctata aaatgttact attggtagat aattacgatt cttttaccta taatttaaag
    17341 caatatttaa gtgtctataa agaaatattc gtcataaaaa atgatgatcc taaccttttt
    17401 ttattagctg agagcgcaga agctatcgtc ttatcacctg gaccaggaca cccaaaggat
    17461 gctgggaaaa tattagaatt aatcaatcag tttataggac agaaacctat tctaggtatc
    17521 tgtttggggc atcaagcgct tgctgagtgc ttgggtgggc gattaaattt agcaaatcac
    17581 gttatgcatg gtaaacaaag ttgggtaaca ataaacgacc atactagttt gtttaaggga
    17641 attgatagtc caacccaagt tatgcgttat cactcattgg tagtgactga tttaccagag
    17701 aatattgcta ttattgcacg atcaaatgag gataatgaaa tcatggcatt ccattgcccc
    17761 agcttaaagg tatatgcgat gcagtttcat cctgagtcaa tcgggagtat tgatggaatg
    17821 aaaatgattg aaaacttttt gacgttaatt aatgactaaa atgtgttagt gtaggataat
    17881 gactctctat aaataaaaga cagtacctaa gggcactgtc ttttatttag ttgtatagat
    17941 gagttaatct gtctgcaaat ttacgataaa taatcaagca aatggatgct tttaaggtat
    18001 ccccaggaat aaaaataagg ttagaaaata gtgatttagt taaggggaga gaagtttgaa
    18061 aggaaagaac gatgctacca caaatatcaa tcaatagtac gccaaatatc caaataataa
    18121 ggaattaaac ccagagtttt gttgttttca ctttctcaag ccctaggccg ataaaaaaag
    18181 ctgcaaatgg ataagtcagc agatagcctg ctgttggccc aaacagcgtt acgagacctg
    18241 accgccctcc tggtaaaatg gtgcaccaat agcaactaag agcaagaaaa tagccacagc
    18301 taggaaacct ttcctactac caagcagagc acctgccagc ataacaccaa gattttgtaa
    18361 gacaattggg actggaatga aacctaatgg gatccctggg ataaaaccta aaacaatgag
    18421 tagtgcaacc atgagtgcaa tataggtagt tgttcttgtt ttcatttaaa ttaattcctt
    18481 tttagtatga tttagttgta atttttccaa gaattcaata tcggtatgtt gattgcgacc
    18541 cccctctgtt aggtaatttc ccaaaaaagt tgagtcaaca agtaaggtga ctaatgattc
    18601 aaaattctca aaatggacct cccgtccagc acttaatctt aattccttga aaggaaaaac
    18661 aaaacgcaac atggccataa tttttaagca tttaatggga gtcaaatagt tatattttcc
    18721 aagaggtgtt ccttcaacag caagtaaaaa attgacaggg atagaatagg ggttcagttc
    18781 ttttagtctg aaagctaatg tgatgagccc ctcatcgctc tctcccatac cacaaatgaa
    18841 tccagaacaa acatcaatgt cttcattgtg aattctttct aaagtatcac aacgatcttt
    18901 gaagctatgc gtcgttgcaa tgttggggta gttttcttcg ggggtgttga ggttatgatt
    18961 gatacggtca attcctgctt gttttagctg atgaagttgt tctctatcca aaaaaccagc
    19021 ggtaaggcat atttctagag gaaggctttt tttgatttca gggatgactt gacataactg
    19081 ctcaatttct ctcttgctag gcttagttcc actcatcgca atacaaaaga cgctagcacc
    19141 attttgatga gcgacaatag cctgttttag aatgacattt tgaggaagca gaccaaattt
    19201 atcaatgtca gcagtgcttt ctttagattg agaacagtaa ccacaatttt ctgcacaaag
    19261 tccttgctta gcacttaata aaacgttaag gcgaatatta tttcggctaa cttgattttt
    19321 aaggtataag gcagcagcgt agatttccca ccagttctca tcgctattta gaatttccag
    19381 cgcttgttca taagaaatgc tagtctttcc agataatatt tcgtcagcca aatggatata
    19441 atttgtttga aatgacatga tagcctcctt taacgaaatt aataatagca taaaaaaact
    19501 gtattgtaaa cttagtctat ataataagtt aacaatataa aaaattatga tatactggtc
    19561 ttatattttg acgggagata agatgaaaaa actatttaaa aaagcgatag ctatgtgccg
    19621 agatttgccg cttaattttg taattgttga acttagtttg tatcaaagag aggatggctc
    19681 atctgataat attaaatgga tttctaagcg aatccttgag gtaacggtaa ctgatcagca
    19741 atggatttac catttgagga tgcttaattt accaaaactt aagccaatgt cactagcatc
    19801 cagtgactgt cctgcaaggg aagagaaggc tagtcttatt tcaatagaag aatgtcaaaa
    19861 attcactagc ttagtgcgag ataaaaatcc ttttcaccaa ggggaagcag ctattgtgcc
    19921 tgcggctcta ttattggaaa aaataatgga agttagaaat ggaaaaataa aggagattca
    19981 tttgactttt ttatctccag taagggtgaa tcaagaattc tcagtgcaag tagtggataa
    20041 cacagtgatt atttttcagc atcaaatatt atgtgtgagg gggacatatg aagaatagag
    20101 atgtttatat tggttttggg ctacgaacac ctatcggtat aaaagggaag caatttaaac
    20161 attaccgtcc agaactttta ggagcacacc tcttaaatca aataaaaaaa atagaatcag
    20221 aatctaatat tgatagtatt atttgtggga acacagttgg tactgggggc aatattggtc
    20281 gtttgatgac tcttttttct gattatgaat cctatattcc agtacaaacg attgatatgc
    20341 agtgtgcttc atcaagttct gccttgtttt ttggttatct aaaaatcagt gccggtatta
    20401 atgaaaaagt tcttgttggg gggattgaaa gtagttctct tcaacctatg agacgttacg
    20461 ctaaagaaga taatcgtaac ggagaatata ccgttgctca gttttctcct gactcttatg
    20521 ctgaaactgt aatgttagaa ggggcacaaa gagtctgtca aaaatatggt tttagaagag
    20581 aaatgttaga taaattggca ttcttgagcc ataaacgcgc cttaacagct aaacaaggtg
    20641 gctatttaga agaggtaatc ttaccaatgg aagggatgcg agatcaaggc gttagaaaac
    20701 taaaagaagc attttttcaa aaattaccaa gattgatgga aaattcacct ttgctcacta
    20761 ttggaaatgt ttgtttaatg cacgatgctg ctgcatttct aacgcttcag agtcagaaaa
    20821 cagaattccg gattgttcac attgtagaag tagcaggaga tcccaagctt agtccagaat
    20881 tggttcacac ggctacggaa aaactattaa cagaaactca tactaaaata tcggattatg
    20941 atgcaattga atggaatgaa ccatttgcag cgattgatgc tttatttaat cattattatc
    21001 ctgaagagag agaaaaattc aatatttttg gaggggcatt agcttacgga cacccttatg
    21061 cctgctcagg aattattaat atccttcatc ttatgcaggc attaaaatat aaaaataaac
    21121 ctatgggtct aactgccatt gcaggggcag gaggagtcgg aatggccata tcaatagagt
    21181 acttaggagt taagaatgct tgaatcattg aaaacaatag ttaaaaccaa tagcgataaa
    21241 aagttatttg atggtgattt acaggtcagt tatggagaat tttataactt agtcagacaa
    21301 gatatggcgt cacaagacaa taggaaacat gtgattagca cacactctct gcttaaccaa
    21361 ttagtaagat ttgtttcaaa gttatgtcaa aaagctcttc ctattatttg taaacctaac
    21421 cttactcata atgaaataag tcgattagaa aaagaagtgc agtatgctcc tcaactagct
    21481 gattttggtg ttctttcttc tgggactaca gctgatgcta aactattgtg gaggagcttc
    21541 gcttcgtggt ctgatttttt tagcattcag aatgcttatt tttcggtcac ttccaatagc
    21601 agactgttta ttcaaggtga tttttcattt actggtaact taaatttagc gctatccctt
    21661 ttattactgg ggggaacgct tgttgtcaca cagaaaaatt ctgtaaaata ctggcagact
    21721 ttatgggaaa aaactggtgt aacgcatcta tatctgctcc ctagttatct caagttagtg
    21781 gaacaatata gcaaggagac tgcgttagac aataaaacta ttattacctc ttcgcaatat
    21841 gtctctgact cattactgga aggtttatat ataaagcatc ctaaagtgtc agtgaaaatc
    21901 ttctatggtg ctagtgaact caattatgtg tcatggtacg atggtcgtga tatccgagat
    21961 aagccacagt atgttggaga aatcgttcca aatgtagctg ttaggattaa agagggaaga
    22021 atttttgtaa aaacaccgta tagtatctgt ggcctttcaa gtgaatattg tgctggtgat
    22081 tatggtgaac tgatagatgg caaattatat ctatttggtc gtggtgacga ttggtgcaat
    22141 caatcgggca ttaagctcta tttacctaga ttaattgaaa aaataaaaac atgcccttat
    22201 atcaaggatg cagtagcatt tacaaaagag agtcaaagtc atggacaaga gtcacattgc
    22261 tgtattgttt taattgaaaa tcagatgcaa caagagtgtt tgaagtggtt atcggaacat
    22321 tttgaaaaga agtatgggtt taagcattat catattgtat ctaaaattcc tttgatgcca
    22381 tctggaaaaa ttgattatca acaactcaaa agacaattag catgagtttt tactaaaaaa
    22441 ataaaatagg gttaatggat tgcaaacctc agttctgata ttctttattt tgaaagatat
    22501 cgtgatataa tgataaaaaa gaaagataag gaataaaatg ttatcaaaag caaaatcacg
    22561 atatattatc cgtgagatta ttaagttatt tccagatgct aaacctagct tagactttac
    22621 taatgtcttt gagcttttgg tagctgtaat gctatcagct caaacaactg atgcagctgt
    22681 taataaagtc actcccgccc tttttgaacg ctttccaaat cctttagttt tggcacaagc
    22741 tgatcccaaa gagattgaac cctatatttc taaaattggg ctttaccgta ataaagctcg
    22801 attcttaaat caatgcgcta aacaattgat agagcatttt gatgggaaag tcccccaaac
    22861 tcgtcaagag ctagaaagtt tgtctggcgt tggaaggaaa acggctaatg tggtcatgag
    22921 tgttggattt ggtattccgg cttttgccgt agatactcat gtcactcgga tttgtaaaca
    22981 ccaccagatt tgcaaacagt cggcttctcc actagaaatt gagaaacgag tcatggaggt
    23041 tttaccgcca gaagagtggc tagctgctca tcaatccatg atttattttg gtcgtgcaat
    23101 ttgtcatcct aaaaatccaa aatgcgacca gtacccacaa ctttatcact ttcctgataa
    23161 tttaaagtga ttagaataaa agagagtaac tggtgacgat tatataagcg atacttaaaa
    23221 acggtagaaa agctatcgtt tttttcattt ttctcataac taaacaataa ataattccta
    23281 accaagcacc tatttgaata ataaaaagca tttgatgaag aggtaaagat aagccaattg
    23341 tggctaaata aaggaaatct ccacttccga tattaatatc aaccacttca gctaaaatcc
    23401 caaataaaca taaaacaata gaaacttttg taatcggtgt gagaat
//
LOCUS       DATRUK010000029        21776 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.029, whole genome shotgun sequence.
ACCESSION   DATRUK010000029 DATRUK010000000
VERSION     DATRUK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21776
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.029"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            124..420
                     /locus_tag="VBE34_001091"
     CDS             124..420
                     /locus_tag="VBE34_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263816.1"
                     /translation="MTEKNYNNHTISLNKTEFFTITDDQVTYTKNEELEKISYDSKNN
                     ELLLNGKKFWTHLTSGELQLTDPKNTDMTLNYASKNSPLFKSYEKGTAKFKEEN"
     gene            680..1012
                     /locus_tag="VBE34_001092"
     CDS             680..1012
                     /locus_tag="VBE34_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263817.1"
                     /translation="MKNLFATTEASSRKQEQDRIVNYIKQHVELTNGNQIKKIEFIDF
                     QKNEMTGTWGISTKINEQFSISFSEDRIGGKLRALGYQPNEIGFSKDINSNNQNVNDI
                     EVIYMKKE"
     gene            1014..1523
                     /locus_tag="VBE34_001093"
     CDS             1014..1523
                     /locus_tag="VBE34_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263818.1"
                     /translation="MISEESYRYLVEDAYRVDSKKVKIPLKRSDIVGNSDYVIIEPPI
                     DNTSNGMQAMVVAPIKEGMTAEPDTSEIVIVYAGTNLGERLDIATDVEMVAGGDTYLL
                     ADPKTKTFRKSQGKSALEYAEKISSKYPNSEITTTGHSLGESEALYVALKMGWMNVGY
                     NGPDIHNMI"
     gene            1694..2080
                     /locus_tag="VBE34_001094"
     CDS             1694..2080
                     /locus_tag="VBE34_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 family protein"
                     /protein_id="HEO7263819.1"
                     /translation="MKKKYWILLILLLTVLSGGGYMVFKQHTEKEKMIAIAHSAEAMQ
                     IYEKHMKANDSRAFSEQGIIHNYKINDSELNYNPMGGMEVTVYVNNDTNKYFQFTILD
                     NGNGKLESGSYIISADLDNDLETNKK"
     gene            2117..3334
                     /locus_tag="VBE34_001095"
     CDS             2117..3334
                     /locus_tag="VBE34_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO7263820.1"
                     /translation="MSEYKTIHPNARLKDQYDNTIGVVSEVYDNTSHSGEQIYAVVDN
                     RHIPKEDVKEVTVLFRGSTSPQEFINNPADVTLDWLENDIPMASNIWVMKDFGNPHNF
                     SAVSPQLKASSEHLKEIMKKYPNAKINIGGHSLGGMDAQYAVVDITDKKDLKRINSVH
                     VYNAPDIYPTLTKGQKKTADSLKSKIVVYVDPNDFIGMVGREGKKGSEDSVGTVYYTE
                     SPDINWIDQHMTYGYRMENGQIKVIETNLPPEVRTTRKAMGSFYKDKKKFKKSGKGLS
                     SHEKIFLDAEQAAVIASGLVSTAETALEDIKTSAKTAVGEAEELWNTTKSIPFGITEL
                     TEAELAEAYAEGGVTYDSIVTKTDTHFDEKVKNAGDLVTTYTTLKSDIQSGVETMLSK
                     DSELAGDFKAWEN"
     gene            3322..3723
                     /locus_tag="VBE34_001096"
     CDS             3322..3723
                     /locus_tag="VBE34_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263821.1"
                     /translation="MGKLDAKDDKLSNSKKDLEKLEDDYHHKTIAISDKFFELEDKRC
                     EFETMLQETYEATSYHLRQDEMINEESFMTMNHIIEAFQSDFDTEYTKEKRRLTALEE
                     ETNQKYSKKRQLLEEKIDHLMSERRDYGNPW"
     gene            3707..4012
                     /locus_tag="VBE34_001097"
     CDS             3707..4012
                     /locus_tag="VBE34_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263822.1"
                     /translation="MGILGDYVLSEASDYISGKKDIKALKKKLDKMLVTGVYAPRIKS
                     KRRSIVSTYDAEITTTATNAKASITAIAGQIDTAIKGQFRTKVETVLDNNSTKYDEI"
     gene            complement(4201..4273)
                     /locus_tag="VBE34_001098"
     tRNA            complement(4201..4273)
                     /locus_tag="VBE34_001098"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4238..4240),aa:Lys,seq:ctt)
     gene            complement(4555..5823)
                     /locus_tag="VBE34_001099"
     CDS             complement(4555..5823)
                     /locus_tag="VBE34_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEO7263823.1"
                     /translation="MADNLALRMRPRNINEVIGQQHLVGNGKIIDRMVAANMLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGNIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKKAIQ
                     LAISDKERGFPFLVTIDDGALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDGSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPEAQIHTVTALEAAQRIGFPEARILIANIVVDLALSPKSNSAYL
                     AMDAALADLRRSGNLPIPRHLRDGHYSGSKTLGNARDYKYPHAYPEKWVKQQYLPDKL
                     VGHNYFEANETGKYERALGSNKERIDKLSD"
     gene            complement(5816..6307)
                     /locus_tag="VBE34_001100"
     CDS             complement(5816..6307)
                     /locus_tag="VBE34_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001879696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7263824.1"
                     /translation="MIIRPMQNCDREAVLTLQNTGWTALTSPVYDHKWTESDLEKNLA
                     NGMSFFVAEIDDKIAGVLDFGPYYPFPAGKHVATFGILIAEPYQGQGLGKALLKALLT
                     EAKAQGYIKIAMHVMGNNSRAISLYQKYGFTEEARITKAFFIENHYVDALIFAKDLEN
                     NHG"
     gene            6634..7104
                     /locus_tag="VBE34_001101"
     CDS             6634..7104
                     /locus_tag="VBE34_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEO7263825.1"
                     /translation="MAKFGFLSVLEEELDKHLQYDFAMDWDKKNHTVEVTFILEAQNS
                     SAIETVDDQGEMSSEDIVFEDYVLFYNPVKSRFDAEDYLVTIPYEPKKGLSREFLAYF
                     AETLNEVATEGLSDLMDFLTDDSIEEFGLSWDTDAFENGRAELKETEFYPYPRY"
     gene            7240..7698
                     /locus_tag="VBE34_001102"
     CDS             7240..7698
                     /locus_tag="VBE34_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7263826.1"
                     /translation="MDIFDELFDFSGAKIALFCEGKILTSLRDDFPDLPYAGFWDLPG
                     DGREGDETPLECLFREVSEELNLTLTRNHIDWVKTYRGMLNPDKLSVFMVGHISQKEY
                     DSIVLGDEGQDYKLMSIDEFLSHKKVIPQLQERLRDYLEVEDDYFTKGGS"
     gene            7670..8140
                     /locus_tag="VBE34_001103"
     CDS             7670..8140
                     /locus_tag="VBE34_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7263827.1"
                     /translation="MITLQKAEASDLEKIIAIQRASFKAVYEKYHDQYDPYVEEVEQI
                     RWKLVERPDCFYHFVLVDETIVGFLRLVIKDEEKRAWLGTAAILPQYQGQGYGSAAMA
                     LLEKTYPKLTKWDLCTIAQEKLMVSFYEKCGYHSTHTEPEQEGMDMVYMTKKIE"
     gene            complement(8179..8895)
                     /locus_tag="VBE34_001104"
     CDS             complement(8179..8895)
                     /locus_tag="VBE34_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7263828.1"
                     /translation="MKTVKEVSILSGVSVRTLHHYDKIGLFPPTALSEAGYRLYDDEA
                     LIRLQEILLFRELEFPLKDIKYLLEQAKEERQDLLAQQIKLLEWKRSHLEQVITHAKR
                     LQEKGYDYMNFDVYNKTELEQLQAEAKEKWGQTAAYQEFAQKHASDDFAQISQEMAKI
                     MVQFGQLKTQNVSDESVQMCVKRLQDYISQHFYTCTNEILAGLGQMYQSDDRFSQSID
                     KAGGAGTSEFVSQAIAYYCQ"
     gene            9019..9477
                     /locus_tag="VBE34_001105"
     CDS             9019..9477
                     /locus_tag="VBE34_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HEO7263829.1"
                     /translation="MEIVELLREWYSSFEVKEEKWNEKYEGILIRIEGDGNYKRCRLA
                     HKTASKMGYFTVFWQKDGNGKNIPFYEQDEVEDLIIVIKDGRRKGLFIIPKEVAVSKG
                     ILSSANSQGKMAMRFYSPWCSDLNRTALVTQRWQLNYFIDLSKNNEGVTT"
     gene            9474..10427
                     /locus_tag="VBE34_001106"
     CDS             9474..10427
                     /locus_tag="VBE34_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEO7263830.1"
                     /translation="MNTWNELTVHVNREAEEAVSNLLIETGSQGVAISDSADYLGQED
                     RFGELYPEVEQSDMIAITAYYPDTLDIEAVKADLADRLANFEGFGLATGSVNLDSQEL
                     VEEDWADNWKKYYEPARITHDLTIVPSWTDYEAKAGEKIIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVIDVGTGSGVLSIASSLLGAKDIYAYDLDDVAVRVAQENIDMNP
                     GTDNIHVAAGDLLKGVQQEADVIIANILADILIHLTDDAYRLVKDEGYLIMSGIISEK
                     WDMVRESAEKAGFFLETHMVQGEWNACVFKKTDDISGVIGG"
     gene            10427..11167
                     /locus_tag="VBE34_001107"
     CDS             10427..11167
                     /locus_tag="VBE34_001107"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEO7263831.1"
                     /translation="MQQYFVNGKADDFVTIEDKDTIKHMFNVMRLTEDDQVVLVFDDA
                     IKRLAKVVDSSAHRFQILEELDSNVEMPVQVTIASGFPKGDKLDFVTQKATELGAAAI
                     WGFPADWSVVKWDGKKLAKKEDKLAKIALGAAEQSKRNRLPQVRLFEKKADFQAELAG
                     FDKIFIAYEESAKEGEMSALAQNLQTVKAGDKLLFIFGPEGGISPKEIAAFEEVGAIK
                     VGLGPRIMRTETAPLYALSVISYSAELL"
     gene            11164..11750
                     /locus_tag="VBE34_001108"
                     /pseudo
     CDS             11164..11750
                     /locus_tag="VBE34_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
     gene            11951..12832
                     /locus_tag="VBE34_001109"
     CDS             11951..12832
                     /locus_tag="VBE34_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEO7263832.1"
                     /translation="MKKHKMLSLLAVSGLMGIGILAGCSNDSSSSSKGTINIVSREEG
                     SGTRGAFIELFGIESKNKKGEKVDHTSDAATVTNSTSVMLTTVSKDPSAIGYSSLGSL
                     NSSVKVLKIDGKNATVKDIKSGSYKISRPFNIVTKEGKEKEATKDFIDYILSKDGQAV
                     VEKNGYIPLDNAKAYQAKVSSGKVVIAGSSSVTPVMEKIKEAYHKVNTKVDVEIQQSD
                     SSTGITSAIDGSADIGMASRELDKTESSKGVKATVIATDGIAVVVNKKNKVNDLSTKQ
                     VKDIFTGKTTSWSDLSK"
     gene            12847..13692
                     /gene="pstC"
                     /locus_tag="VBE34_001110"
     CDS             12847..13692
                     /gene="pstC"
                     /locus_tag="VBE34_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO7263833.1"
                     /translation="MKQTKEGLMQAVFFITACVSVISAILICLFLFSSGLPAITKIGW
                     GNFIFGKVWHPSNNIFGIFPMIVGSLYVTAGALLLGGPIGILTAVFMAYFCPENIYRP
                     LKSAINLMAGIPSVVYGFFGLVVIVPMIRQYIGGFGMGVLAASILLGIMILPTIVSIS
                     ESSLRAVPESYYEGGIALGASHERSVFFAVLPAAKRGILASVVLGIGRAIGETMAVIM
                     VAGNQAVLPQSLTSGVRTLTTNIVMEMGYSSGLHRQALIGTAVVLFIFILMINISFSA
                     LQKEE"
     gene            13694..14572
                     /gene="pstA"
                     /locus_tag="VBE34_001111"
     CDS             13694..14572
                     /gene="pstA"
                     /locus_tag="VBE34_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO7263834.1"
                     /translation="MENINQRTSKQYLASLKKDPLSFFLFAIVYLGAILSFATIAFVV
                     IYILVKGLPHVNTGLFAWTYNTQNVSLLPAFINTIFIIALTLLFAVPLGIGGSIYLTE
                     YARRDNPYLKIIRVATETLAGIPSIIYGLFGALFFVKYTHLGLSLISGSLTLSIMILP
                     LIMRTTEEALLSVPDSYREGAFALGAGKLRTIFKIVLPSAMSGIFAGIILAIGRIIGE
                     SAALIFTAGTVAKVVHSVFSSSRTLAVHMYAISGEGLYVDQTYATAVILLLLVIIVNF
                     VSGLVAKRLGSESNSE"
     gene            14565..15314
                     /gene="pstB"
                     /locus_tag="VBE34_001112"
     CDS             14565..15314
                     /gene="pstB"
                     /locus_tag="VBE34_001112"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO7263835.1"
                     /translation="MSKLVINNLDLYYGEFHALKDVNLDIEEKEITAFIGPSGCGKST
                     LLKSINRMNDLVKNCKITGDITLEGEDVYRQLDINQLRKKVGMVFQKPNPFPMSIYDN
                     VAFGPRTHGIHSKAELDDIVERSLKQAALWDEVKDRLHKSALGMSGGQQQRLCIARAL
                     AIEPDVLLMDEPTSALDPISTAKIEELVIQLKKNYTIVIVTHNMQQAVRISDKTAFFL
                     MGEVVEYNKTSQLFSLPQDERTENYITGRFG"
     gene            15311..15967
                     /gene="phoU"
                     /locus_tag="VBE34_001113"
     CDS             15311..15967
                     /gene="phoU"
                     /locus_tag="VBE34_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO7263836.1"
                     /translation="MIRSRFASQLNDLNKEIIFMGALCEDIIGKSLGALTNSNDVYLD
                     DISETYHKIEQMERDIEERCLKLLLRQQPVAKDLRRISSALKMVYDMKRIGAQAYEIA
                     EIVSLGHIIQGAGSERDQLNSMSNNVISMLTKSIDAFIYDNEEQAHQVIEQDRTVNQE
                     FDTIKKQLVLYFSVQDVDGEYPIDVLMIAKYLERIGDHTVNIAKWVLFSITGNLDGEA
                     "
     gene            15967..16644
                     /locus_tag="VBE34_001114"
     CDS             15967..16644
                     /locus_tag="VBE34_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7263837.1"
                     /translation="MIYCVEDDADIREMMLYTLQMAGFTAQGFSSSELFWEAVQEKVP
                     DLILLDIMLPGDDGLTILERLRRKHQTEMIPVIMTTAKGSEYDKVKGLDLGADDYLVK
                     PFGMMEMISRIKAVLRRSRQVDSKAHIIIGNLEIDPTNYWVKRGTEKIHLTLKEFELL
                     VLFFRNPNRVFTRQELLDKVWGEQFLGETRTVDVHIGTLRTKLGEDGYLIATVRGVGY
                     RLEERHD"
     gene            16637..18292
                     /locus_tag="VBE34_001115"
     CDS             16637..18292
                     /locus_tag="VBE34_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO7263838.1"
                     /translation="MTKKIFRTTLSASLGIVLVTILMIMGFLYNYFNRIQREQLRTQT
                     ALASQGISFEGKDYFENLKTSNVRITWVDNKGQVLYDTQSDAKHMKNHANRQEIKEAI
                     KSGYGESTRWSATLTEKSIYAAQRLNNGTIVRLSVAQQTIFYLLLGMMSPLAIIILLA
                     IILSVLIARYIAKKVSEPLNNIDLDHPLSNDSYEEITPLLRRLDSHQAKIQHQKLLLQ
                     KRQKEFDTIISKIKEGMILLDDQARIVSINAEALKLFQINDDWHGRFMMEVSRDLTLK
                     DLIDQGLKGKKKEANIDIENNHYRVLVRPTTDNNRVTGLVVLLFDVTDQLQMEQLQRE
                     FTANVSHELKTPLHVISGYSELLANQMVPNEEVPQFAAKIHKESERLVKLVEDIINLS
                     HLDEQEKLPQETVNLYDLTQKVLEGLQAKADKKHIQINFNGEEAILRGNPVLLNSLVY
                     NLCDNAITYNHEKGQVNVTLKNSPDTITLEVSDTGLGIAEKDKKRIFERFYRVDKSRS
                     KIVGGTGLGLSIVKSALDFHNGSIKVDSHLGQGTTMTVLLHKQ"
     gene            18447..20630
                     /locus_tag="VBE34_001116"
     CDS             18447..20630
                     /locus_tag="VBE34_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO7263839.1"
                     /translation="MKNNVKQLFSFEFWQKFGKALMVVIAVMPAAGLMVSIGNSIRLL
                     DPSNVLLGRIANVIAQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLSFILINLI
                     TGNFFGVKTDMLANSKATVQTVFGATIRVSDYFVNVLGQPALNMGVFVGIISGFVGAT
                     AFNKYYNYRKLPDALTFFNGKRFVPFVVIYRSVIVALILSVFWPVVQSGINGFGKWIA
                     SSQDSAPILAPFVYGTLERLLLPFGLHHMLTIPMNYTQLGGTYTVLTGATKGAQVLGQ
                     DPLWLAWVGDLINLKGSNSSQYHHLLTSVTPARFKVGQMIGASGILMGLSYAMYRNVD
                     KDKKLKYKSMFISAAAATFLTGVTEPIEYMFMFAAMPLYLVYAVVQGCAFAMADIVNL
                     RVHSFGNIEFLTRVPMGIKAGLGGDIFNFVWVTLLFAVLMYFIANFMIKKFNLATAGR
                     NGNYDNEEVDNAPSTASGSADANSQVVQVINLLGGRDNIEDVDACMTRLRVTVKDGNS
                     VGSEAAWKKAGAMGLVLKGNGVQAIYGPKADVLKSDIQDLLDSGTVIPIVDLETGQPV
                     AATQVTTYKGITEEIVSVANGQVEALDVVKDPVFSQKMMGDGFAVEPTDGNIYVPVSG
                     TVTSVFPTKHAFGLLTESGLEVLVHIGLDTVALDGQPFEVKISSGQKVVAGDLAVVAD
                     LEAIKAAGKETSVIIVFTNVSDIKTVKLEKSGPQIAKTVVAKVEL"
     gene            20669..21499
                     /locus_tag="VBE34_001117"
     CDS             20669..21499
                     /locus_tag="VBE34_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEO7263840.1"
                     /translation="MKGYSMAKCLTLNTHSWMEVNALKKLFDLAEHIFREKYDIICLQ
                     EVNQSISSPLATSSPNYHPIEGTPALHQDNFALQLVHYLNLQGLHYHWTWAYNHIGYS
                     KYHEGVAILSLKPLKPDDILVSAVDDETDYHTRRALVAETTLNDKVVTVVSLHLSWFE
                     KGFAEEWKRLETTLLEVETPLLLMGDFNNPTGNQGYELVLNSPLALKDSHQIANHVFG
                     DHTIMADIDGWEGNKKALKVDHIFTSEDLSISSSQVVFEGGEAPVVSDHYGLEITLNL
                     "
BASE COUNT         7082 a         3419 c         4561 g         6714 t
ORIGIN      
        1 acaattgtaa taaggagaaa agatgaaaaa aaatatttat tacttcttcc aatagtatta
       61 attatagtgg tagtagttgg catacttaat cataaaaaaa tgccagatga aggaagatat
      121 tatttgactg aaaaaaatta taataatcat actatctcat taaataaaac tgaatttttt
      181 accataaccg atgatcaagt tacctataca aaaaatgagg agttagaaaa aatatcttat
      241 gatagcaaaa ataatgaatt gcttttaaat ggtaaaaagt tttggactca tctcacttct
      301 ggtgagcttc agttaacaga tcctaagaac actgatatga cattaaatta tgctagtaaa
      361 aatagtcctt tattcaaatc atatgaaaaa ggaacagcaa aatttaaaga ggaaaattaa
      421 attatattat taactgctga ataagttatg gtgcaagaca tacttgtggg agtgttaagg
      481 ttcaagaagg agtagtattg aatcttcaat tatttgggaa tctgaattac cttcttcaag
      541 aaaaaagagg taatttaatt aatttaaaag aagttatcta tatttgaatg gtatagaaaa
      601 aacagaggtt tatttgctta tgaaaaaacg tatatggtat ttgataataa taatcacagt
      661 aattttagga ggactagcca tgaaaaactt atttgcaaca acagaagcat catcaaggaa
      721 acaggaacaa gatagaattg tcaattacat aaaacaacat gttgagttaa caaatggtaa
      781 tcaaataaaa aaaattgagt ttatcgactt tcaaaaaaat gagatgacag gtacatgggg
      841 aatttctact aaaattaatg aacaattttc gattagtttt tctgaagata gaattggtgg
      901 taaacttaga gcattaggat atcaaccgaa tgaaataggt ttttcaaagg acatcaatag
      961 taataatcaa aatgttaatg atattgaagt gatttatatg aagaaagaat agaatgattt
     1021 cagaagaaag ttacagatat ttagttgagg atgcttatcg agttgattca aaaaaagtaa
     1081 aaatacctct gaaaagaagt gatattgtag ggaacagtga ctatgttatt attgaaccac
     1141 ctatagataa tacttcaaat ggtatgcaag ctatggtagt tgctccgata aaagaaggaa
     1201 tgacagctga accagatact agtgaaatag tgattgttta tgcaggtact aatttaggtg
     1261 agcgtttaga catagctaca gatgttgaaa tggttgctgg aggggataca tatttacttg
     1321 ctgatcctaa aaccaagact tttaggaaat ctcagggaaa atcagcacta gagtacgcag
     1381 aaaaaatatc gagtaaatat cctaattcag aaattactac aaccggtcac tccttagggg
     1441 aatcagaggc actatatgtc gctttaaaaa tgggttggat gaatgttggt tataatggtc
     1501 cagatattca taacatgata taaaatgacg aaatagatta tatgaaatct catccagaat
     1561 aatttagaaa aaaatcgaaa aatatgagtt gtttgagtta aaattttaac tatggagata
     1621 gagtcctgta ttatttgatt ttgtagtaaa ctagttagga atttattcat aggatattat
     1681 aaaggagtta atgatgaaga aaaaatattg gatattacta atattactat taacagtttt
     1741 atcaggaggc ggatatatgg tttttaaaca acatacagaa aaagaaaaaa tgattgcaat
     1801 tgcacacagt gcagaagcga tgcaaattta tgagaagcat atgaaagcaa atgattcgcg
     1861 agctttttca gagcaaggaa ttattcataa ttataaaatt aacgattcag aactcaatta
     1921 taatcctatg ggaggaatgg aggttactgt ttatgtgaat aatgatacca ataaatattt
     1981 tcaatttact attttagata atggaaatgg taaacttgaa tctgggagct atattataag
     2041 tgcagattta gataatgatt tagaaaccaa taaaaaatga aaaaaatata ttgatgaaga
     2101 aaaattacaa atcgcaatgt cagaatataa aacaatccat cccaacgcca gattaaagga
     2161 tcaatatgat aacacaattg gagttgtttc ggaggtttac gacaatactt ctcattctgg
     2221 agaacagatt tatgctgtgg ttgataatcg tcatattcca aaagaagatg taaaagaggt
     2281 gacggtactt tttcgtggat caacatctcc tcaggaattt attaataatc ctgcagatgt
     2341 gactttggat tggttagaaa atgacattcc tatggcaagt aatatttggg taatgaaaga
     2401 ttttgggaat cctcataatt tcagtgctgt atcaccacag ttgaaagctt catcagaaca
     2461 tttaaaagag attatgaaaa aatatccaaa tgcaaaaata aacattggtg gacattcatt
     2521 aggaggtatg gatgctcaat atgctgttgt agatattaca gataagaaag acttgaaacg
     2581 gattaactct gtacatgttt ataatgctcc agatatatat cctacactta ctaaaggaca
     2641 gaaaaagacg gcagattcac tgaaatctaa gattgttgtt tatgttgatc ctaatgattt
     2701 tattggcatg gtggggcgtg aaggaaaaaa aggaagtgaa gactctgtag gaacagtcta
     2761 ttatacagaa agtccagata tcaactggat agatcaacac atgacctatg gctatcgtat
     2821 ggaaaatggt cagattaaag ttattgaaac gaatttacca ccggaagtta ggactactag
     2881 aaaagctatg ggatcttttt ataaggacaa gaaaaaattt aaaaaatctg gtaagggtct
     2941 aagttcgcat gaaaaaatct tcttggatgc tgaacaagct gctgttattg caagtggttt
     3001 agtctctact gctgaaactg ctttagagga tatcaagaca tctgcgaaaa cagctgttgg
     3061 agaagcagaa gaattgtgga atacgactaa atcgattcct tttggtatta cggagcttac
     3121 agaagcagag ttggcagaag cttatgcaga aggtggtgtc acttatgata gcatagtaac
     3181 taaaactgat actcattttg atgagaaagt caaaaatgca ggagatttag taacaacata
     3241 tacgactttg aaaagtgata ttcaatcagg tgttgaaacc atgttatcta aggatagtga
     3301 attagctgga gattttaaag catgggaaaa ttagacgcta aagatgataa attaagtaac
     3361 agtaaaaagg atttagaaaa gttggaagat gattaccatc ataaaaccat agctatttca
     3421 gataaattct ttgaactgga agataaacga tgtgagtttg agacgatgct tcaggaaact
     3481 tacgaagcaa caagttatca tcttcgtcag gatgagatga ttaatgaaga gtcctttatg
     3541 accatgaatc acattataga agcttttcaa agtgattttg atacagaata tactaaagag
     3601 aaaagaagat tgactgcctt agaagaagaa actaatcaaa agtactctaa aaagagacaa
     3661 ttgcttgaag aaaaaataga tcatttaatg tcagaaagga gagactatgg gaatccttgg
     3721 tgattatgtt ttatcagaag cctctgatta tatcagtgga aaaaaagata tcaaggcttt
     3781 aaaaaagaaa cttgataaaa tgttagtgac aggtgtttat gctccaagaa tcaagtctaa
     3841 acgtagaagt attgtctcta cctatgatgc agagataacg actactgcaa caaacgcaaa
     3901 agctagtata actgccattg caggtcaaat agacactgca ataaagggac agtttagaac
     3961 taaagttgaa actgtattgg ataataacag tacaaagtat gatgaaattt aaaagacatg
     4021 ggctaaataa tttagctctt tttttatact caaaaataag agaaatacaa aacaaaatac
     4081 atatttatgc aagtaaagag aaataaaaaa atcaccattt tatctgtcaa aaaaaattgg
     4141 tgggcaagtg taaaaagagc gtcaaaaaag cctataggta taggctttgt tttataagac
     4201 tgccccctac agggctcgaa cctgtgaccc atagattaag agtctactgc tctaccaact
     4261 gagctaagga ggctaggaag ctgtattggc accgatgatc cactttttgt attgatcccg
     4321 cacaaaataa gcaggtgggt aactcgcacc tctttaagtg gcttccgcgt gttaacggct
     4381 tgcgcactag agatgacttc gtttcccgaa atacaaagaa atagtcggtc aacacttaga
     4441 tgtggattcg tatgccacag caatgtttct atgtctttat gataccactt tttataaaat
     4501 tttcaagcaa aattatcgat ttttgaaatc gattgcgatt tttaaaactg gcaatcagtc
     4561 tgagagctta tcaatgcgct ctttatttga accaagtgca cgttcgtatt taccagtttc
     4621 attggcttca aaataattat gtcctactag tttatctggt aagtattgtt gtttaaccca
     4681 tttttcagga taggcgtgag ggtacttgta atcccttgca ttgcccaagg ttttactacc
     4741 gctgtaatgt ccatctctta ggtgacgtgg aataggtaaa tttcctgaac gacgtaaatc
     4801 agctaaggct gcatccatag ccaaataggc tgaatttgat ttaggtgata gagctaaatc
     4861 cacaacaatg ttagcaataa gaatacgagc ttctggaaaa ccaatgcgtt gggcagcttc
     4921 caatgcagtc acagtatgta tttgtgcttc ggggttagct aagccgatat cttcataagc
     4981 aataatggtc aaacgtcttg ctaaactagg taaatctcca gcttctacta aacgagcggc
     5041 atagtgcagg ctggcattga catcagaacc tctaattgat ttttgtaagg ctgataggac
     5101 atcataatgc ccatctccgt ttttatccat tgtaatataa ctacgttgca gactattttc
     5161 cattgtctct agactaatat gacggctacc atcttcgtta ggagaagtag acatgactgc
     5221 tagatccaag gaattataag cagatcgtag gtctccattt gtggcggtaa caatgaaatc
     5281 tagcgcccca tcatcaattg tcactaaaaa aggaaatccc cgttctttat cggaaattgc
     5341 tagttgaatg gcttttttaa tatcctcgtt agaaagaggt tctagctcaa aaatttgtac
     5401 cctacttcta atggcaggag ttactgaaaa gaaaggattt tcagttgttg ctccaatcat
     5461 aataatattg ccattctcaa gtaaaggtaa aagaaaatct tgttttgtct tgtcaagtct
     5521 atgaatctcg tcaagtaata aaactaagcc accagaaaat ttagcttctt ctgcaatttc
     5581 ttgaagacgt tttttactat caacagtggc attaaaagta cgaaaggcat atttagtagt
     5641 accagcaata gcagaagcaa tagaagtctt cccaatacct ggtggtccat ataggatcat
     5701 ggaagataac atattagctg caaccattcg atcaataatt ttgccattac caacgagatg
     5761 ttgctgccca atgacttcat taatattgcg aggacgcata cgtagagcaa gattgtcagc
     5821 catgattatt ctccaaatct tttgcgaaaa ttaaagcatc aacataatga ttttcaatga
     5881 aaaaggcttt agtgattcga gcctcttcgg taaaaccgta tttttgatag agggaaatgg
     5941 ctctactatt gttgcccatg acgtgcatgg caatcttgat gtaaccttga gctttagcct
     6001 cagttagcag agctttcaag agagccttac ctagcccctg accttggtag ggttcagcga
     6061 tgagaatgcc gaaagtggca acatgcttgc cagctgggaa aggatagtag ggaccaaagt
     6121 ccaagacgcc agcgatctta tcatcgatct cagcgacaaa gaaggacatg ccatttgcca
     6181 gattcttctc caagtcactc tcagtccact tgtggtcata gacaggagag gtaagagcag
     6241 tccagccagt attttgtaag gtcagaactg cctcacggtc gcagttctgc ataggtctga
     6301 taatcataat tgatgataca aagggacggt tcggtttacg aaaattttcg taaagaaata
     6361 gggaacgacg aagtgtcgtc agacactagg aagttcatct tttcttttag aaaattagtc
     6421 ccgtgttcaa atcctttcta ttttttatat agagtttttc tctcagctga ctcttttaac
     6481 atagtatgaa gtcagtcaaa aacgactagt ctttgagact attgtaacat aaaaataagt
     6541 aggaactacc ttttgattaa cctgagtctg taacgttttt cttctatatt catgttataa
     6601 taagaaagaa taaatagaaa ataaggacat aagatggcta aatttggatt tttatcggta
     6661 ctagaagaag aattggataa gcatttacag tatgactttg ccatggattg ggataagaaa
     6721 aatcatactg tagaagtcac ttttatatta gaagcacaaa acagctctgc tattgaaacc
     6781 gtggacgacc agggggagat gagtagcgag gacatcgttt ttgaggacta tgtactcttt
     6841 tacaaccctg tcaagtctcg ctttgatgct gaggactatc tggtgactat cccctatgag
     6901 cctaagaagg gcttgtcgcg tgagtttctg gcttattttg cggagactct caacgaggtt
     6961 gccactgaag ggttgagcga cctcatggac ttcctcaccg atgacagtat cgaagagttc
     7021 ggtctttctt gggatacgga tgcttttgaa aatggcagag cagaactgaa agagacagag
     7081 ttttaccctt atccgagata ttagacttga tggatttgct aaaatgtgga tatacattgg
     7141 atgatataga aacagccaga ccagaggata tggaacgtta ttatccccct gaacaaatca
     7201 gaaaatatgg agctttagga atagaattga ggttattgca tggatatttt tgatgagtta
     7261 tttgatttct caggtgcaaa gatcgctctt ttctgcgagg gaaagatttt gaccagccta
     7321 agagatgatt ttccagattt gccctatgca ggcttttggg acttaccagg cgatggtcgt
     7381 gagggcgatg aaacaccttt ggaatgtctt tttcgagaag ttagtgaaga attaaatctg
     7441 accttaactc gaaaccatat tgattgggtc aagacttata ggggtatgct gaaccctgat
     7501 aaattatcgg tttttatggt tggacatatc agtcagaaag agtacgatag cattgttcta
     7561 ggcgatgaag gacaagacta caagctcatg tccattgacg aatttttaag ccataaaaag
     7621 gtcattcctc agttgcagga gaggttgaga gattatttgg aggtggaaga tgattacttt
     7681 acaaaaggcg gaagctagtg atttagagaa gattattgcc attcagagag ctagctttaa
     7741 ggcagtctat gagaaatacc acgatcaata cgatccctat gtggaagaag ttgagcagat
     7801 tcgatggaaa ctagtggagc gaccagattg tttctatcat tttgtccttg ttgatgagac
     7861 aattgttggt tttcttagat tggttatcaa ggacgaggag aagcgtgctt ggcttggtac
     7921 ggcggctatt cttccccaat atcaaggaca gggctacggc tcagcagcga tggctttatt
     7981 agagaaaacc tatcctaagc ttacaaagtg ggatttatgt acgattgcgc aagaaaaact
     8041 aatggtcagt ttttatgaaa aatgtggtta tcattcaacc cataccgaac ctgagcaaga
     8101 aggaatggat atggtctata tgacaaagaa gatagaataa aaaaagaaaa ctagatgatt
     8161 gtctagtttt ctgatagttt attggcaata gtaagcaata gcttggctga caaattcact
     8221 tgttcctgct ccaccagctt tgtcaattga ttgactaaac cggtcatctg attgatacat
     8281 ttgtcctagt ccagcaagga tttcattggt acaggtgtaa aaatgctgag agatatagtc
     8341 ttgtagccgt ttcacacaca tttgaacaga ttcatcagat acattttgtg tctttaattg
     8401 cccaaattgt accattattt ttgccatttc ttggctaatt tgtgcaaagt catctgaagc
     8461 atgtttttga gcaaattctt ggtaggctgc tgtttgtccc catttttctt ttgcttcagc
     8521 ttgtaattgt tctaattctg ttttattata aacatcgaaa ttcatataat catatccttt
     8581 ttcttgtaat cgtttcgcat gggtgataac ctgctcaaga tgcgatcttt tccattcaag
     8641 caatttgatt tgttgggcta ataagtcttg cctttcctct tttgcttgct caagcaggta
     8701 tttgatatct ttgagaggga attccaactc acgaaatagc aaaatttctt gcaggcgaat
     8761 caatgcttca tcgtcataaa gtcgatagcc tgcttcggat agggcggtag gtgggaataa
     8821 accgatttta tcatagtgat gtaaggtgcg cacacttaca ccgcttaaga tactgacttc
     8881 tttaactgtt ttcatggtaa taacctcctc tcacttacat agtataaagg ataacctaag
     8941 gtcagggtca agcaaaaaat aaattatttt gaaaagtatg gttaatcata ttgatttgaa
     9001 gataaaggag ggatagttat agaaatagtt gagctactaa gggaatggta ttcttccttt
     9061 gaagttaaag aagaaaagtg gaatgaaaaa tatgaaggta tcctcataag aattgaaggc
     9121 gatggaaact ataaaaggtg tcgtttagct cataagacag ctagcaagat ggggtatttt
     9181 acagtatttt ggcagaaaga tggaaatggt aaaaatattc ctttttatga acaagatgaa
     9241 gtggaagatt tgattattgt cattaaagat ggtaggagga aaggtttgtt catcattccc
     9301 aaagaggttg cagtgagtaa gggtattctt tcatctgcta actctcaagg aaagatggct
     9361 atgcgatttt actctccttg gtgtagcgat ttaaatagaa cagctttggt gacacagcga
     9421 tggcagttga actattttat tgatttaagt aaaaataatg aaggagttac tacatgaaca
     9481 catggaatga actaacggtt cacgtgaatc gtgaggcaga ggaagctgtc tcgaatttat
     9541 tgattgaaac tggcagtcag ggggttgcca ttagcgactc ggctgattat cttggacagg
     9601 aggatcgttt cggcgagctt tatcctgagg tggagcaatc tgatatgatc gctattactg
     9661 cctattatcc tgatacgctt gatattgagg cggttaaggc tgatctggct gatcgtttgg
     9721 ctaattttga aggctttggt cttgcgacgg gtagcgtcaa ccttgacagt caggaattgg
     9781 ttgaagagga ctgggctgat aactggaaga agtactatga gccagctcgc attacccatg
     9841 atttgaccat cgtgccgtca tggacggact acgaggctaa ggctggtgag aagattatca
     9901 agctggatcc tggtatggct tttgggactg ggactcaccc aacgactaag atgagtcttt
     9961 ttgccttgga gcaagtgctt cgtggtggtg agacggttat cgatgtgggg actggttctg
    10021 gcgttttgtc tattgctagc tctctcttgg gtgccaagga catctatgct tatgatttgg
    10081 atgatgttgc ggtgcgcgtg gctcaggaaa atatcgatat gaaccctggt actgataata
    10141 ttcatgtggc tgctggtgac ctgctcaaag gtgtccagca agaggcagat gtcattattg
    10201 ccaatatttt ggcggatatt ttgatccatc tgacagacga tgcctaccgt ttggtcaagg
    10261 atgagggtta tctcatcatg tctggtatca tttctgaaaa atgggacatg gtgcgtgaga
    10321 gtgctgaaaa agctggtttc ttccttgaga ctcacatggt tcaaggtgag tggaatgcct
    10381 gtgttttcaa aaagacagat gatatttcag gagtgattgg cggctaatgc aacagtattt
    10441 tgtaaatggc aaagcagatg actttgtgac catcgaggac aaggatacca tcaagcacat
    10501 gttcaatgtc atgcgattga cagaagatga ccaagtggtc ttggtttttg atgatgccat
    10561 caagcgtctg gcaaaggtgg tggacagctc tgcccatcgc ttccaaattc tagaagagct
    10621 agacagcaat gttgaaatgc ctgtccaagt gaccatcgcc tcaggttttc ccaagggaga
    10681 caagttggat tttgtgacgc aaaaggcaac agaattgggt gctgcggcta tctggggatt
    10741 cccagctgac tggtcagtcg tcaagtggga cggcaagaaa ctagctaaaa aagaagataa
    10801 gctggcaaaa attgctctgg gcgcagcgga gcagagcaag cgtaatcgtc ttcctcaggt
    10861 tcgtttattt gagaagaagg ctgattttca agcggagctt gcaggctttg ataagatttt
    10921 tatcgcctat gaagagtctg ccaaggaggg tgaaatgtca gcgttagccc agaatttgca
    10981 gactgtaaaa gcaggtgaca agctcctctt catctttgga ccagaagggg gcatttctcc
    11041 aaaagaaatc gcagcttttg aagaggttgg agctatcaag gtgggcctgg gtccacgcat
    11101 catgcgcaca gaaacagcac cgctctatgc tctaagtgtt attagttact cagcggagtt
    11161 attatgatta ttgctattca aggtgatatg gctgtaggga aaaccacagc tttgaaaatc
    11221 attgagaaag tttacccgaa aatcgctatt agttttgagg atatttcacc agtagttgat
    11281 aagattaata aacatcagct tgataacaat aaattagacg attattgtga aattcaacaa
    11341 ctatttattc agcatgagcg agatagatac gaacttgtta gagataatga aatagtgtta
    11401 atggattatg gagctgagga aattgctttc tatacacaat actttccaaa ggtggcgggt
    11461 tatagttgac tttaccttct aatttgttac tagaggtaaa taggctaaag gaatgttttg
    11521 ctgattatat tatttttcta gatgcagatg agacggtcct ataccaacgt caaaaaaatg
    11581 attttaatag acggcgtcat tttttcaaat ggtatactca tcacctgttg cctgctaaaa
    11641 aacattattt agacacattt tccaatgtta ttcatattaa tacaaatcat ttaaatgctg
    11701 aagaagtggc tgaaaaaatc attgacatca ttcgtaagtt aactctataa tgggatagtt
    11761 atacttcaat tatgtggtat aacaactgtt tcaagccgac ttattggctg aattaccata
    11821 atagaaaaaa ggagtttgga taattttcca actcttttta attttacatt gattttacaa
    11881 aaatcctcaa agagatttga tattccttaa ctataataaa atcaaatcaa aaaatattta
    11941 ggagttcatc atgaaaaaac acaaaatgct atctcttttg gcggtatctg gtcttatggg
    12001 aattggcatc ctagcaggat gctcaaatga ttcatcatct tcatctaaag ggacaattaa
    12061 cattgtttct agagaggaag ggtctggaac tcgtggagct ttcatagagt tatttgggat
    12121 cgagtcaaaa aataaaaagg gcgaaaaagt agaccatact tctgatgcag caactgttac
    12181 caattcaact tcagttatgc taaccactgt ctctaaagac ccgtcagcta ttggttattc
    12241 ttcactaggt tcattaaata gtagcgttaa agtgcttaaa attgatggca aaaatgcgac
    12301 tgtaaaagat attaaatcag gaagctataa gatttctcgt cctttcaata ttgtaacaaa
    12361 agaaggtaag gaaaaagaag caacaaaaga ttttattgat tatatcttaa gcaaagatgg
    12421 gcaagcagtc gttgagaaaa atggctatat tccacttgac aatgctaaag catatcaagc
    12481 aaaagtgtca tctggtaaag ttgttatagc aggttcaagt tcagtaacgc cggttatgga
    12541 aaagattaaa gaagcctatc ataaagtaaa tacaaaagta gatgttgaaa ttcaacaaag
    12601 tgattcttca actgggatta caagcgcaat tgatggatca gcagatatag gtatggcatc
    12661 acgtgaattg gataagacag agtcatccaa aggggtcaaa gctactgtta ttgcaacaga
    12721 tggtattgca gtagttgtta ataagaaaaa taaagttaat gatctatcaa caaaacaagt
    12781 aaaagacatt tttactggaa agacaacgtc ttggtctgac ttatcaaagt aaggtaagag
    12841 taaacaatga aacaaacaaa agaaggtctt atgcaagctg tgtttttcat cacggcttgc
    12901 gtcagtgtta tatcagctat tttaatttgc ctctttctct tctctagtgg tcttccagct
    12961 attacaaaaa tcggctgggg aaattttata tttggtaaag tctggcatcc tagtaataat
    13021 atttttggca tctttccaat gattgtaggt tccttatatg ttacagcggg agctttactt
    13081 ttaggaggcc cgataggtat ccttactgct gtttttatgg cttatttctg cccagaaaat
    13141 atttataggc ctttaaagtc agctatcaat ttaatggcgg gaattccttc agttgtttat
    13201 ggtttctttg gactcgttgt cattgtccct atgataaggc aatatattgg tggctttggt
    13261 atgggagtct tagcagcttc aattttgcta ggaattatga ttttaccaac aattgttagc
    13321 atttcagaat cttctttaag agctgttcca gagagctatt atgaaggcgg tattgccctt
    13381 ggtgcctctc atgaacgaag tgtcttcttt gctgttttac cagctgctaa acgtggtatt
    13441 ttagcatcgg ttgttttggg aattggacgt gctattggtg aaacaatggc tgtcatcatg
    13501 gtagcgggta atcaagctgt cttgcctcaa tcgctgacat caggcgttag aactctaacc
    13561 actaatattg tcatggaaat gggatattct tctggtttgc atcgtcaagc cttaattggt
    13621 acagctgtgg tactctttat ctttatatta atgattaata tcagtttttc agcattacag
    13681 aaagaggaat aagatggaaa atattaacca aagaacgtca aaacaatatt tagcatcttt
    13741 aaaaaaagat cccctttcct tctttctttt tgctattgtc tatttgggag caattctaag
    13801 ttttgctaca attgcatttg ttgttattta tattttagtt aaggggcttc ctcatgtgaa
    13861 tactggcttg tttgcatgga cttataatac tcagaacgtc tcactattac cagctttcat
    13921 taacactatc tttatcatcg ccttaacttt attatttgca gttccccttg gtattggtgg
    13981 atcaatttat ctaacggaat atgctagacg tgataatcct tatctcaaaa ttatccgtgt
    14041 tgcaaccgag acattggcag ggataccttc aattatttat ggtttatttg gtgcattatt
    14101 ttttgtaaaa tacacacact taggcttatc tttgatatcg ggttctctga ctttgagcat
    14161 catgatttta cctttgatta tgaggacaac tgaagaagct ttactatctg ttccggattc
    14221 ttatcgagaa ggagcctttg cgcttggtgc tggtaaattg agaaccatat ttaaaatcgt
    14281 attacctagt gctatgtctg gtatttttgc tggtattatt ttagcgatag gacgtatcat
    14341 tggtgagtca gcagcactta ttttcacagc tggaacagtt gcaaaagtag tacattcggt
    14401 atttagttca tctcgtactt tagcagtaca tatgtatgct atttcgggtg agggcttata
    14461 tgttgaccaa acttatgcga ctgcggtcat cttattgctt ttagttatta ttgtcaattt
    14521 tgtatctgga ctagtagcca aacgattagg aagtgagtcg aacagtgagt aaattagtaa
    14581 ttaataattt agatttgtat tatggagaat ttcacgctct gaaggatgtt aatttagaca
    14641 ttgaagaaaa agaaatcaca gcctttattg gaccttctgg gtgcggaaag tctactctct
    14701 tgaaaagtat taaccgtatg aatgatttgg tcaaaaattg caaaatcaca ggggatatta
    14761 ctttagaagg cgaggatgtc tatagacaat tggatattaa ccaattgcgt aaaaaggttg
    14821 gaatggtgtt tcaaaaacct aatccgtttc caatgagcat ttatgataat gttgcttttg
    14881 gtccaagaac gcacggtatt cattccaaag cagagttaga tgatatcgtt gaacgttccc
    14941 ttaaacaggc ggctctatgg gatgaggtta aagatcgtct tcacaaatct gctctgggta
    15001 tgtctggcgg acagcagcaa cgtttgtgta ttgcgcgtgc tcttgctatt gagcctgatg
    15061 ttttacttat ggatgaaccc acaagtgcct tggatcctat ttcaacagca aaaattgaag
    15121 aattggttat ccaactgaag aagaactaca ctattgttat agtgacacat aatatgcaac
    15181 aagctgttcg tatttctgat aaaacagcat tctttctgat gggagaagtg gttgaatata
    15241 acaaaacgag ccaactcttt tcattgccac aagatgagcg tacagaaaac tacattacag
    15301 ggagattcgg atgattcgtt cacgatttgc aagtcaactt aatgacctta ataaagaaat
    15361 tatttttatg ggggcattgt gtgaggacat cattgggaag tccttaggtg ctctaacaaa
    15421 tagtaatgat gtttaccttg acgatatttc tgaaacttat cacaagattg aacaaatgga
    15481 acgggatatt gaggagagat gtttaaaact tttacttcgt cagcaaccag ttgccaagga
    15541 tttacgccgt atttcttctg cattaaaaat ggtttatgat atgaaacgta ttggtgctca
    15601 agcttatgaa attgcggaaa ttgttagttt aggtcatatt atccaaggtg caggttctga
    15661 aagggatcaa cttaacagca tgtctaataa tgtcattagt atgctgacta agagtataga
    15721 tgcttttatt tatgacaatg aagaacaagc gcatcaagta attgaacaag ataggacagt
    15781 taaccaggaa tttgatacaa taaagaaaca gttagtgtta tatttttcgg tacaggatgt
    15841 tgatggtgaa tatcccatag atgttttaat gatcgccaag tacctagagc gtattggtga
    15901 tcatacagta aatattgcta aatgggtgct tttctcaata acaggtaacc tagatggaga
    15961 agcgtaatga tatattgtgt tgaagatgat gctgatattc gcgaaatgat gctctatact
    16021 ttgcaaatgg caggttttac agctcaagga ttctcgagtt cagagctttt ttgggaagct
    16081 gttcaggaga aagttccaga cttgatttta cttgatatta tgttgccggg agatgatggt
    16141 ttgactattt tagagcgttt aaggagaaaa catcaaacgg aaatgatacc agtaatcatg
    16201 acaactgcta aaggtagtga gtatgataaa gttaaagggt tagatcttgg agcagatgat
    16261 tatctcgtca aaccttttgg gatgatggaa atgatttcac gaataaaagc agtcttgaga
    16321 cgtagccgcc aagtagattc aaaagctcat attatcattg gaaatttaga gattgacccg
    16381 actaattatt gggtcaaaag gggaacggaa aaaatccact taaccctaaa ggaatttgag
    16441 ttattagtat tattcttccg taatcccaat agagtcttta caaggcaaga acttctggat
    16501 aaggtttggg gagaacaatt tttaggagaa actagaactg tggatgttca cattgggaca
    16561 ctgcgaacaa aacttggtga ggatggctat ttgatcgcta cagtaagggg agttggatac
    16621 cgtttggagg aaagacatga ctaaaaaaat tttccgtaca actttatccg ctagtttggg
    16681 aattgtttta gttacaattt tgatgattat gggatttctc tataattact tcaatcgcat
    16741 tcagcgagaa cagttgagaa cacaaacggc actggcgtca caaggaattt cctttgaagg
    16801 taaagattat tttgaaaatc ttaaaacttc aaatgtgcgt attacttggg tagataataa
    16861 gggacaagtg ctttatgata cccaatcaga tgcaaaacat atgaaaaatc acgccaatcg
    16921 tcaagaaatt aaagaagcta tcaagagtgg ttatggtgag agcacgaggt ggtcagcgac
    16981 acttactgag aagtctatct atgctgcgca acgtttaaat aatggaacga tagttcgttt
    17041 atcagttgcg caacaaacta ttttttatct tctccttggt atgatgagtc ccttagcaat
    17101 tatcatctta cttgctatta ttttatctgt attaattgca cgatatatag caaaaaaagt
    17161 atcagagcct ttaaacaata ttgacttaga tcatccttta tcaaatgata gttacgagga
    17221 gataacacca ttattaaggc gtcttgatag ccatcaggct aagattcaac atcaaaaact
    17281 gttattacaa aagcgtcaaa aagaatttga taccattatt tctaaaatta aggaaggaat
    17341 gattctttta gatgatcaag caagaatcgt tagtatcaac gcagaagctt taaagttatt
    17401 ccaaattaat gatgattggc atgggcgctt tatgatggaa gttagccgag atttaactct
    17461 caaagactta attgatcaag gactaaaagg gaaaaagaaa gaggctaata ttgacattga
    17521 aaataatcat taccgcgtct tggtaagacc aactacagat aacaaccgag taacaggatt
    17581 ggttgttcta ctgtttgatg tgactgacca actccaaatg gaacaacttc aacgtgaatt
    17641 tactgccaat gtctctcatg aattgaaaac cccgcttcat gttatttctg gttactcaga
    17701 actgttagct aatcaaatgg ttccaaacga agaagtccca caatttgcag ctaaaattca
    17761 taaagaatct gaacgattag ttaaattggt tgaggatatt atcaaccttt ctcaccttga
    17821 tgaacaagaa aaattaccgc aagagacagt aaatctgtat gacttaacac aaaaagtttt
    17881 agaaggttta caagctaaag cagataagaa gcacatacaa atcaatttta atggagaaga
    17941 agctattttg cgagggaatc cagttttatt gaattccttg gtgtacaact tgtgtgacaa
    18001 tgctatcacc tataatcatg agaaaggtca agtaaatgtg actttgaaaa attcacctga
    18061 tacaattacc ttagaagtta gtgatacggg actaggcatt gcagagaagg acaaaaaacg
    18121 tattttcgaa cgtttctatc gcgtcgataa aagtcgctca aaaatagttg gagggactgg
    18181 tttaggtttg tctattgtaa agagtgcttt agattttcac aatggtagta taaaagtgga
    18241 cagccatttg gggcagggaa caacaatgac cgttttactt cacaaacaat aaaaactcac
    18301 aaataaaagt ctagatgata ttatctagac ttttttagta attcagaaaa aatatataag
    18361 taagggttta caaaaaacaa ataagtgcta taataaaacc ataacgcaaa cgtttgcact
    18421 attaagtgca taaaaggaga ttgaagatga aaaataatgt caaacagctc tttagttttg
    18481 aattttggca gaagtttggg aaggctttga tggttgttat tgctgttatg ccagcagcag
    18541 gattgatggt tagtattggt aactccatta ggctattgga tccatcaaat gttttattag
    18601 ggcgcatagc taatgttatt gctcaaattg gttggggagt tattggtaac ttacacatct
    18661 tatttgcatt ggctattggt ggtagctggg ctaaagaacg tgccggtggt gcttttgcgg
    18721 ctggcttatc ctttattctt attaacctta ttacagggaa cttttttggt gtgaaaacag
    18781 atatgttagc aaacagtaaa gcaactgttc aaacggtatt tggtgcgacc atcagagttt
    18841 cagattactt tgttaacgtt cttggacaac ctgctcttaa tatgggtgta tttgtcggta
    18901 ttatttcagg tttcgttggt gcaactgcct ttaataaata ttataactat cgtaaattac
    18961 cagatgcttt aacttttttc aatggaaaac gttttgttcc atttgttgtt atttatcgtt
    19021 cagtcattgt tgcattaatt ttatcagtat tttggccggt tgttcaatca ggtatcaatg
    19081 gctttggtaa gtggattgca agttcacaag acagtgctcc aatattagca ccatttgtct
    19141 acggtacgtt agagcgttta ttgctcccat ttggtttaca ccatatgtta actattccaa
    19201 tgaactacac gcaactaggt ggcacataca ctgtccttac aggtgcaact aaaggagcac
    19261 aggtgttggg acaagatcca ctgtggttag catgggttgg tgatttaatc aacttaaaag
    19321 gctcaaattc aagtcaatac catcacttat tgacaagtgt cactccagca cgttttaaag
    19381 tggggcaaat gattggtgct tcaggtattt tgatgggatt atcttatgct atgtatcgta
    19441 acgttgataa agataaaaaa ttgaaataca agtcaatgtt tatttcagca gcagcggcta
    19501 ctttcttgac aggtgttact gagccaattg agtacatgtt catgtttgca gcaatgccac
    19561 tctaccttgt atatgctgtc gttcaaggat gtgcgtttgc tatggcagat attgttaacc
    19621 tccgcgttca ttcatttggt aatatagaat ttttaacacg tgttcccatg ggaataaaag
    19681 cgggccttgg tggtgatatt ttcaactttg tttgggtgac acttcttttt gcagtattga
    19741 tgtactttat tgcaaacttt atgattaaaa aattcaattt agcaacagca ggacgtaatg
    19801 gtaattatga taacgaagag gttgataatg ctccttcaac agcttctggt tctgctgatg
    19861 caaattctca agttgtacaa gttattaatt tacttggtgg tcgtgataac attgaagatg
    19921 tagatgcatg tatgactcgt cttcgtgtca cagttaaaga tgggaatagc gtgggttctg
    19981 aagcagcttg gaaaaaagct ggtgcaatgg gattggtgtt aaaaggtaat ggcgttcaag
    20041 ctatttacgg acctaaagca gatgtcctta aatcagacat tcaagatttg ctagattcag
    20101 gtacagtgat tccaatagtt gatttagaaa caggccagcc tgtagcagca acccaagtta
    20161 caacttataa aggaataaca gaagaaattg tttcggtggc taatggtcaa gtagaagcat
    20221 tggatgttgt taaagaccct gtcttttctc aaaaaatgat gggtgatggc tttgctgtgg
    20281 aaccaactga tggcaatatc tatgttccag tttcaggtac agttactagt gtctttccaa
    20341 caaaacatgc ttttggttta ttaacagaaa gtggcctaga agttttagta catatcggtc
    20401 tagatacagt agctcttgat ggtcaaccat ttgaggttaa aatttctagt ggtcaaaaag
    20461 tggtagcagg cgatcttgct gtagtagcag atttggaagc tatcaaagca gctggtaaag
    20521 agacaagtgt gattatcgtc tttacaaatg tttcagatat aaaaactgtt aaacttgaaa
    20581 aatcaggtcc acaaatagct aaaacagttg tggcaaaagt cgaactgtaa caaacgatag
    20641 gagctgggca agtctcagct tctttctgat gaaaggatac tctatggcaa aatgcttaac
    20701 gttaaatact cattcatgga tggaagtaaa tgctctaaag aaattatttg atttagcaga
    20761 gcatatcttc cgtgaaaaat atgatatcat ctgcctacaa gaggtgaacc agagcatatc
    20821 ttcccctctt gctacgagta gtccaaatta tcatccgatt gaaggtacac cagctcttca
    20881 tcaagataac tttgccttgc aattagtgca ttacctaaat cttcaaggtc tacactatca
    20941 ttggacatgg gcttataatc atattggtta tagtaagtac catgaagggg tagcgatact
    21001 ttctctgaaa ccattaaagc cagatgatat tttagtttca gctgtagacg atgagactga
    21061 ttatcatact cgtcgtgctc tggtagctga gaccactctg aatgacaaag tcgttacagt
    21121 agttagcctc catttatcat ggtttgagaa aggatttgcg gaggagtgga aacgactaga
    21181 aactacccta cttgaggtag aaactccact tttactcatg ggtgacttta ataatcctac
    21241 tggaaatcag ggttatgaac ttgttcttaa tagtccatta gcattaaagg atagccacca
    21301 aatagccaat catgtatttg gagaccatac tattatggct gatattgatg gttgggaagg
    21361 gaataaaaag gctcttaaag ttgaccatat ttttactagc gaagaccttt caatcagttc
    21421 aagtcaagta gtttttgaag gtggtgaagc acctgtcgtt agtgatcatt acggcttgga
    21481 aattacgtta aatctttaga ttaatctatt taatatatta aaagcatcct aaatttgtct
    21541 ataaaacaaa acttagggtg ctttttgaaa aatatgtcac attatttaac ttcttacgaa
    21601 gtttggaatg catgagaaaa tatgataaaa tgataataac tattataggt gattttaata
    21661 aataatttaa tcaattggtt tatctattag aacaattaat acgaggagac gagagtgaat
    21721 atttttattt tagaggataa ttttattcaa caggcacgtt tagagagaat aattga
//
LOCUS       DATRUK010000030        21664 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.030, whole genome shotgun sequence.
ACCESSION   DATRUK010000030 DATRUK010000000
VERSION     DATRUK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21664
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.030"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..77
                     /locus_tag="VBE34_001118"
     CDS             <1..77
                     /locus_tag="VBE34_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7263841.1"
                     /translation="GERFAGKKKFIRNIQPFFNAFKAD"
     gene            199..891
                     /locus_tag="VBE34_001119"
     CDS             199..891
                     /locus_tag="VBE34_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO7263842.1"
                     /translation="MSKVRLYIARHGKTMFNTIGRAQGWSDTPLTTFGELGIKELGWG
                     LKASNISFKEAFSSDSGRTLQTMEIILREVQQENIPYTRDKRIREWCFGSLDGGYDGD
                     LFNGVLPRVSNGDMSHLTHEEIANLICQVDTAGWAEPWAILSNRILSGFTAIAKKIED
                     IGGGNAIVVSHGMTIATFLWLIDHSTPRSLGLDNGSVSVVDFEDGTFSIQSIGDMSYR
                     EKGREILEKTLQ"
     gene            888..1640
                     /locus_tag="VBE34_001120"
     CDS             888..1640
                     /locus_tag="VBE34_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEO7263843.1"
                     /translation="MKKNKIIRFSLVGVLLAILCFSLFALLKPNSQQSSSQKLRNEDI
                     KKTSSQKRNKKLRLPAVSSKDWNLILVNRDHKHEELSPDVVPVENIYLDKRITKQATQ
                     FLEAARAIDSREHLISGYRSVAYQEKLFNSYVTQEMTSNPNLTRGQAEKLVKTYSQPA
                     GASEHQTGLAMDMSTVDSLNESDPRVVSQLKKIAPQYGFVLRFPDGKTAETGVGYEDW
                     HYRYVGVESAKYMVKHHLTLEEYITLLKENNQ"
     gene            1637..2212
                     /locus_tag="VBE34_001121"
     CDS             1637..2212
                     /locus_tag="VBE34_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEO7263844.1"
                     /translation="MRKRFSLLNFIVVTFIFFFFILFPLLNHKGKVDANSRQSVTYTK
                     EEFIQKIVPDAQDLGKSYGIRPSFIIAQAALDSDFGEKILANKYHNLFGLLAEPGTPS
                     ITLNDSSTGKKQEKQFTHYKSWKYSMYDYLAHIKSGATGKKDSYTIMVSVKNPKTLVQ
                     KLQDSGFDNDKKYAKKMTEIIDLYDLTRYDK"
     gene            2307..3389
                     /gene="hrcA"
                     /locus_tag="VBE34_001122"
     CDS             2307..3389
                     /gene="hrcA"
                     /locus_tag="VBE34_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEO7263845.1"
                     /translation="MCKSANFRKTERGDVFVITQRQNDILNLIVELFTQTHEPVGSKA
                     LQRTIDSSSATIRNDMAKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQD
                     IYHVIKAFDFEAFKLEDMLQKASHILSEMTGYTSVILDVEPARQRLTGFDVVQLSNHD
                     ALAVMTLDESKPVTVQFAIPRNFLTRDLIAFKAIVEERLLDGSVMDIHYKLRTEIPQI
                     VQKYFVTTDNVLQLFDYVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLR
                     QGLKESEMASVQVADSQEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLV
                     NIIGKVLAAKLGDYYRYLNSNHYEVH"
     gene            3392..3964
                     /gene="grpE"
                     /locus_tag="VBE34_001123"
     CDS             3392..3964
                     /gene="grpE"
                     /locus_tag="VBE34_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEO7263846.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEEIVEEVIEEIP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            4145..5974
                     /gene="dnaK"
                     /locus_tag="VBE34_001124"
     CDS             4145..5974
                     /gene="dnaK"
                     /locus_tag="VBE34_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEO7263847.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVEEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIQSNSGLTDEEIDKMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKKAQESGNLDDMKAKLEALNEKAQ
                     ALAVKLYEQAAAAQQAAQGAEGAQSADSSSKGDDVVDGEFTEK"
     gene            6263..7378
                     /gene="dnaJ"
                     /locus_tag="VBE34_001125"
     CDS             6263..7378
                     /gene="dnaJ"
                     /locus_tag="VBE34_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEO7263848.1"
                     /translation="MNNTEFYDRLGVSKDASQDEIKKAYRRMSKKYHPDINKEAGAEE
                     KYKEVQEAYETLSDTQKRAAYDQYGAAGANGGFGGFDGGGFGGFEDIFSSFFGGGGMR
                     NPNAPRQGDDLQYRVNLSFEEAIFGAEKEVSYNRESSCHTCSGSGAKPGTSPVTCQKC
                     HGSGVINVDTQTPLGTMRRQVTCDVCQGSGQEIKEKCPTCHGTGHEKKTHKVSVKIPA
                     GVETGQQIRLTGQGEAGFNGGPYGDLFVIINVLPSQQFERNGSTIYYTLNIGFVQAAL
                     GDTIDIPTVHGAVEMSIPAGTQTGKTFRLRGKGAPKLRGGGQGDQHVTVNIVTPTKLN
                     DAQKEALHAFAEASGDKMVHPKKKGFFDKVKDALDVD"
     gene            7496..8743
                     /locus_tag="VBE34_001126"
     CDS             7496..8743
                     /locus_tag="VBE34_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEO7263849.1"
                     /translation="MVTKVEEIRSYLIASIQNGKLAPGDRLPSIRQLANQFSCNKDTV
                     QRVLMELRFDNYIYAKPRSGYYVFDSHQEEVEEGVSLPNSEIANIAYDDFRLCLNETL
                     IGREDYLFNYYYRQEGLLDLSKAVAKLMEETGVYVPLDDIVITAGTQQALFILTQVTF
                     PNRKSRVLIEEPTYPRMIELIKTQNLPYETIPRGTHGIDFQRLEEIFQTQSIKFFYVI
                     PRMHNPLGTSYNPVEMKRLIEMAEKYDVYIVEDDYMSDFASQSPLHYYDTHGRVIYLK
                     SFSKAIFPALRLAAICLPQALKSTFMAYKKLMDYDTNLILQKALALYIENGLYAKNSQ
                     YLKYRYQKDLANSKSILADHPNLPPYSLHHDSVLFDCSKLDNFKILRQYGDTLENYFC
                     QKSHQSLLQVKNDSCLKQFLGSL"
     gene            8814..9590
                     /gene="truA"
                     /locus_tag="VBE34_001127"
     CDS             8814..9590
                     /gene="truA"
                     /locus_tag="VBE34_001127"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEO7263850.1"
                     /translation="MTRYKAQISYDGSAFSGFQRQPNCRTVQEEIERTLKRLNSGNDV
                     IIYGAGRTDAGVHAYGQVIHFDLPQARDVEKLRFGLDTQCPDDIDIVKVEQVSDDFHC
                     RYDKHIKTYEFLVDIGRPKNPMMRNYATHYPYPVIIELMQEAIKDLVGTHDFTGFTAS
                     GTSVENKVRTIFDAKIQFEASKNLLIFTFTGNGFLYKQVRNMVGTLLKIGNGRMPISQ
                     IKTILQAKNRDLAGPTAAGNGLYLKEIIYEDEECFSNFRK"
     gene            9553..10311
                     /locus_tag="VBE34_001128"
     CDS             9553..10311
                     /locus_tag="VBE34_001128"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO7263851.1"
                     /translation="MKTRNVLAISGNDIFSGGGLHADLATYVVNKLHGFVAVTCLTAM
                     SDKGFEVIPIEASILKQQLESLKDVEFGSIKLGLLPNVETAQVVLEFVKSKQECPVVL
                     DPVLVCKENHDLEVSQLREQLIAFFPYADVITPNLVETQLLTGLSIENLDQMKIAAEK
                     LYDMGAKHVVIKGGNRLNAEEATDLYYDGERFETYVFPVVDANNTGAGCTFASSIASQ
                     LAMGKNVEDAVKMSKGFVYQAIKASDKYGVVQHF"
     gene            10321..10785
                     /locus_tag="VBE34_001129"
     CDS             10321..10785
                     /locus_tag="VBE34_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7263852.1"
                     /translation="MRKEKTSQLVQLAIVTAISIVLGMFISIPTPTGFLTLLDAGIFF
                     AAFYFGKKEGAVVGALAGFLIDLLKGYPNWMFFSLLIHGAQGYLAGLPGRRRLLGLIS
                     ATLVMVLGYAIASGLMYGWGAVLPDIPGNIMQNMVGMVVGFALNKSLERVKK"
     gene            10782..11351
                     /locus_tag="VBE34_001130"
     CDS             10782..11351
                     /locus_tag="VBE34_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEO7263853.1"
                     /translation="MILDYNKLKQETKAIVVDIIERSALKKGQIFVLGLSSSEVSGGL
                     IGKNSSSEIGEIIVEVILKELHSRGIYLAVQGCEHVNRALVVEAELAERQQLEVVNVV
                     PNLHAGGSGQVAAFKLMTSPVEVEEIVAHAGIDIGDTSIGMHIKRVQVPLIPISRELG
                     GAHVTALASRPKLIGGARAGYTSDPIRKF"
     gene            complement(11388..12230)
                     /locus_tag="VBE34_001131"
     CDS             complement(11388..12230)
                     /locus_tag="VBE34_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEO7263854.1"
                     /translation="MITLEKFIDHLNVEEVLFTFFTKLISILLLIIAFVIVRQVINYL
                     FEKTVNRSLAFSRQKVARQKTLAKLSHNVLNYTLYFFLFYWILSILGVPISSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILLENQFDVGDIINVGAVSGTVTNVGIRTTQIHDFD
                     GTLHFIPNRNITIVSNKSRSNMRAQIDIPLFVHTNLDQISDIVTKINEEYVSKHPAIV
                     GEPTVFGPTTNANGQFVYRINIFTQNGAQFDIYAEFYKLYQKAILEEGIDLPTYNFSN
                     NHSR"
     gene            12387..13670
                     /locus_tag="VBE34_001132"
     CDS             12387..13670
                     /locus_tag="VBE34_001132"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEO7263855.1"
                     /translation="MSTSFENKATNRGIITFTISQDEIKPALDQAFNKVKKDLNVPGF
                     RKGHMPRTVFNQKFGEEALYENALNLVLPKAYEAAVAELGLDVVAQPKIDVVSMEKGQ
                     DWKLTAEVVTKPEVKLGDYKDLSVEVDVSKEVSDEEVDAKVERERNNLAELTVKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEEQLVGSKAGQTVDVNVT
                     FPEDYQAEDLAGKDAKFVTTIHEVKTKEVPALDDELAKDIDDEVETLDELKAKYRKEL
                     ESAKEIAFDDAVEGVAIELAVANAEIVELPEEMVHDEVHRAMNEFMGNMQRQGISPEM
                     YFQLTGTTEEDLHKQYQADADKRVKTNLVIEAIAAAEGFEATDEEIEKEITDLASEYN
                     MEADQVRGLLSADMLKHDIAMKKAVDVITSSATVK"
     gene            13859..14434
                     /locus_tag="VBE34_001133"
     CDS             13859..14434
                     /locus_tag="VBE34_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEO7263856.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILEQRGRDNEMYFSDLVNDIQT
                     YLGKSDSAIRESLPFFYSDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     APKRKKKRVNAFMDGDEDAIDYNDDDPEDEDFTEETPSLEYDEENPDDEKSEVESYDS
                     EINEIIPDEDLDEDVEINEEEEEEEEEEEEV"
     gene            14707..16311
                     /locus_tag="VBE34_001134"
     CDS             14707..16311
                     /locus_tag="VBE34_001134"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEO7263857.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLKKE
                     RRGEYLGATVQVIPHVTDALKEKIKRAATTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAG
                     QSIKNKLAQFCDVAPEAVIESLDVDHIYQIPLNMQAQNMDQIVCDHLKLETPAADMTE
                     WSAMVDKVMNLEKKVKIALVGKYVELPDAYLSVVEALKHSGYVNDVAIDLKWVNAAEV
                     TEDNIKELVGDADGIIVPGGFGQRGSEGKIEAIRYARENDVPMLGVCLGMQLTCVEFA
                     RNVLNLHGANSAELDPKTPFPIIDIMRDQIDIEDMGGTLRLGLYPCKLKAGSRAAAAY
                     NNQEVVQRRHRHRYEFNTKFREQFEAAGFVFSGVSPDNRLMEVVELPEKKFFVAAQYH
                     PELQSRPNHAEELYTAFVTAAVENMK"
     gene            16420..17346
                     /locus_tag="VBE34_001135"
     CDS             16420..17346
                     /locus_tag="VBE34_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7263858.1"
                     /translation="MKKIRLSKFIKMIVVILFLISVAASFYFFHVAQVRDDKSFISNG
                     QRKPGNSLYAYDKSFDKLLKQKIEMTNQNIKQVAWYVPAAKKTHKTVVVVHGFANSKE
                     NMKAYGWLFHKLGYNVLMPDNIAHGESHGQLIGYGWNDRENIIKWTEMIVDKNPSSQI
                     TLFGVSMGGATVMMASGEKLPSQVVNIIEDCGYSSVWDELKFQAKEMYGLPAFPLLYE
                     VSTISKIRAGFSYGQASSVEQLKKNNLPALFIHGDKDNFVPTSMVYDNYKATAGKKEL
                     YIVKGAKHAKSFETEPEKYEKRISSFLKKYEK"
     gene            17393..17478
                     /locus_tag="VBE34_001136"
     tRNA            17393..17478
                     /locus_tag="VBE34_001136"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17427..17429,aa:Leu,seq:aag)
     gene            17541..17987
                     /locus_tag="VBE34_001137"
     CDS             17541..17987
                     /locus_tag="VBE34_001137"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEO7263859.1"
                     /translation="MSKVRGFELVSQFSNKELLPKRETAHAAGYDLKVAKKTVIEPGE
                     ITLVPTGIKAHMQPGEVLYLYDRSSNPRKKGIVLINSVGVIDGDYYNNQVNEGHIFAQ
                     MQNITDQAVILEEGERIVQAVFAPFLLADDDQATGMRTGGFGSTGK"
     gene            18149..19513
                     /gene="radA"
                     /locus_tag="VBE34_001138"
     CDS             18149..19513
                     /gene="radA"
                     /locus_tag="VBE34_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEO7263860.1"
                     /translation="MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKN
                     ARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
                     KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFGNAKRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQESFIGE
                     IGLTGEIRRVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDVIGVTTVSQVLKA
                     VFST"
     gene            19649..20146
                     /locus_tag="VBE34_001139"
     CDS             19649..20146
                     /locus_tag="VBE34_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEO7263861.1"
                     /translation="MTTYFDNFLKTNQAYADLHGTAHLPIKPKTKVAIVTCMDSRLHV
                     AQALGLALGDAHILRNAGGRVTADVLRSLVISQQQLGTREIVVLHHTDCGAQTFTNEA
                     FAAQLQRDLGVDMHGHDFLPFNDIEESVREDVAKLHASPLIPNDVVISGAIYDVDTGR
                     MVEVK"
     gene            20300..21619
                     /locus_tag="VBE34_001140"
     CDS             20300..21619
                     /locus_tag="VBE34_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEO7263862.1"
                     /translation="MKKYDVIVLGFGKAGKTLAAKLATQGKSVAMVEEDDKMYGGTCI
                     NIGCIPTKTLLVSASKNHDFQEAMTTRNEVTSRLRAKNFAMLDNKDTVDVYNAKARFI
                     SNKVVELTGGADKQELTADVIIINTGAKSVQLPIPGLADSQHVYDSTAIQELAHLPKR
                     LGIIGGGNIGLEFATLYSELGSKVTVIDSQSRIFAREEEELSEMAQDYLEEMGISFKL
                     SADIKSVQNEDENVVISFEDEKLSFDAVLYATGRKPNTEGLALENTDIKLTERGAIAV
                     DEYCQTSVENIFAVGDVNGGPQFTYISLDDSRIVLNYLNGDKDYSLKNRGAVPTSTFT
                     NPPLATVGLDEKTAKEKGYQVKSNSLLVSAMPRAHVNNDLRGIFKVVVDTETNLILGA
                     RLFGAESHELINIITMAMDNKIPYTYFQKQIFTHPTMVENFNDLFNF"
BASE COUNT         7105 a         3345 c         4585 g         6629 t
ORIGIN      
        1 atggtgaacg ttttgcgggg aagaaaaaat tcatacgaaa tattcaacct tttttcaatg
       61 cttttaaagc ggattaatcg attttatttc tgcagtagat agctgcagaa tttttttata
      121 gggaaagttc gctgatatat tcctgtgaaa gcctttgttt tttgttataa tataataaaa
      181 tgcttgtgag gtgtcagtat gagtaaagtt cgtttatata tcgctcgtca tggtaaaaca
      241 atgtttaata caattggtcg tgctcagggg tggagtgata ctcctttaac tacttttgga
      301 gaactcggga ttaaagagct tggttgggga ctaaaggctt caaatattag ttttaaagag
      361 gctttttcta gtgatagtgg acgtacttta caaactatgg aaattattct cagggaagtg
      421 caacaagaaa atattcccta tacgagagat aaacgaatcc gcgagtggtg ctttggtagt
      481 ttggatggag gatatgatgg cgaccttttt aatggcgttc tgcctcgagt ttcaaacgga
      541 gatatgagcc atttgacaca cgaagaaatt gctaatttga tttgtcaagt tgatacggct
      601 ggctgggcag aaccgtgggc aatattgagt aaccgtattc tgtcaggctt cactgctata
      661 gctaaaaaaa tagaggatat tgggggaggt aatgcgattg ttgtcagtca tgggatgact
      721 attgcgactt ttttatggtt aatcgaccat agtacccctc gtagtttagg cttagataat
      781 ggtagtgtct cagtcgttga ttttgaagat ggaacatttt ctattcaatc tattggggat
      841 atgtcctatc gagaaaaagg aagagaaatt ttagaaaaaa cattgcaatg aaaaaaaata
      901 aaattatccg attcagttta gttggtgttc tacttgcgat actatgcttt agtctttttg
      961 ctttattgaa gcctaacagt caacaatcat catctcaaaa gttgaggaat gaggatataa
     1021 aaaagacatc ctctcaaaaa agaaataaga aattacgatt accagctgta tcatcaaaag
     1081 attggaactt gattttggtc aatcgtgacc ataaacatga agaattaagt ccagatgtgg
     1141 tgcctgttga aaatatttat ttggataaac gtattacgaa gcaagctact cagtttttag
     1201 aggctgctag agcaattgat tcacgagaac atttaatttc gggttatcgt agtgttgcct
     1261 atcaggagaa gttgttcaat tcttatgtta ctcaagagat gactagtaac cctaatttga
     1321 cgaggggaca agcagaaaag ttggtaaaaa cttactctca gcctgcaggt gctagtgaac
     1381 accagactgg attagcgatg gatatgagta ctgtagattc tttgaatgag agcgatccta
     1441 gagtagtcag tcagttgaaa aagatagctc cacaatatgg ttttgtctta cggtttccgg
     1501 atggtaaaac agcagaaaca ggggtaggtt atgaagattg gcattaccgc tatgttgggg
     1561 tagagtctgc aaaatatatg gtcaaacatc atttaacatt agaagaatac ataactttat
     1621 taaaggagaa taaccaatga ggaaacgttt ttccttgcta aattttattg ttgttacttt
     1681 tattttcttt ttctttattc tttttccgct tttaaaccat aagggaaaag tagatgctaa
     1741 ttctaggcag agtgttacct acaccaaaga agaatttata caaaaaattg tgccagatgc
     1801 gcaagatcta ggaaagtcgt acggtattcg tccttcattt attattgcac aggcggcttt
     1861 ggattctgat ttcggagaga aaatattagc taataagtat cataatctgt ttggtttgct
     1921 tgcagaacca ggaacgccct caattacctt aaatgatagt agtacaggaa aaaaacagga
     1981 aaagcaattt actcattata aatcttggaa gtattcaatg tatgattacc ttgctcatat
     2041 aaaatctgga gcgacaggca aaaaagattc atatactata atggtgtctg ttaaaaatcc
     2101 aaaaacttta gtgcaaaaat tacaagatag tggttttgat aatgacaaaa agtacgctaa
     2161 aaaaatgacg gaaatcattg atttgtatga tttaacaaga tatgataagt gatattagca
     2221 ctctttgaat aagagtgcta atttttgatg ttttttcctt gactatcttt taaaaaggag
     2281 tataataaaa tcatagttag cagtcgatgt gtaagagtgc taatttcaga aagaccgaga
     2341 ggggtgatgt ttttgtgatt acacaacgtc aaaatgatat tttgaattta atcgttgagt
     2401 tatttacaca aactcatgaa ccagtagggt ctaaagcttt acagagaacg attgattcta
     2461 gtagcgccac tattcgtaat gatatggcaa aactagagaa gttggggtta ttggaaaaag
     2521 cacacacttc tagtggacgg atgccgagtc cagctggttt taagtatttt gtagagcact
     2581 cccttagact agatagtatt gatgagcaag atatttatca tgttataaaa gcttttgact
     2641 ttgaagcatt caaattagaa gacatgcttc aaaaagctag tcatatttta tcggagatga
     2701 cgggctatac atccgttatt ttagatgtgg agccagctcg tcaacgtttg acagggtttg
     2761 atgttgttca gcttagcaac catgatgctc tagcagtcat gactttagat gaatctaagc
     2821 cagtgacagt gcagtttgct attccaagaa actttttgac acgtgacttg atagcattta
     2881 aagctatcgt agaggagcgc ttgctggatg gtagtgtcat ggatattcat tataagttga
     2941 ggacagaaat tcctcagatc gttcagaagt attttgtaac tacggataat gttttacaac
     3001 tatttgatta tgttttttca gagctgtttt tggaaacggt atttgtagct ggcaaggtaa
     3061 attctttaac atattcagat ctatcaacgt atcagttttt ggataatgag cagcaagtag
     3121 caatttcttt gaggcaaggt ctgaaagaga gtgagatggc ttctgtccaa gtggcagata
     3181 gtcaagaagc cgcacttgca gatgtgtcag ttttaactca caaatttttg attccttacc
     3241 gtggatttgg actcctgagt ttgattggtc ctattgatat ggattatcgt aggtctgtta
     3301 gtttggtaaa cattatcgga aaggtattgg ctgctaaatt aggtgattat tatcgatacc
     3361 taaattctaa ccattatgaa gttcactgat aatggcagta ttcaataagt tatttaaaag
     3421 gaggcactca gtgtcagaag aaattaaaaa agacgattta caggaagaag tggaagccac
     3481 tgaaactgaa gagatagttg aggaagttat tgaagaaatt cctgaaaaat cagagttaga
     3541 actcgcaaac gagcgtgctg atgagtttga aaataaatac ttgcgtgctc atgcagaaat
     3601 gcagaatata cagcgtcgct ctagtgaaga acgtcaacaa cttcaacgct atcgttctca
     3661 ggatctggca aaagcaatct taccatcgct tgacaatcta gaacgtgcct tggctgttga
     3721 agggttgaca gatgatgtta aaaaaggttt agaaatgact cgtgatagcc ttattcaagc
     3781 actaaaggaa gaaggcgtag aagaagtaga agtcgactca tttgaccaca actttcatat
     3841 ggcagttcag accctcccag ccgatgatga acatccagcc gatagcattg cagaagtctt
     3901 tcaaaaggga tataaattgc acgagcgtct gctaagacca gcgatggtag ttgtctataa
     3961 ctaaggtaaa tgagttttcg tttttgtccg taatgacagt aaactagata gcaagttaga
     4021 agctattcag cttgctgatt aaactatagt gattgcttag aattggaagt aaaataattc
     4081 gagtgcttac taagataaat tgaaataaaa agtaataaag tattataaaa taagaggtat
     4141 taacatgtct aaaattattg gtattgactt aggtacaaca aactcagcag tagcagttct
     4201 tgaagggact gaatcaaaaa tcattgctaa cccagaaggc aatcgtacaa ctccttcagt
     4261 agtatcattc aaaaatggtg agattatcgt gggtgatgct gcaaaacgtc aagcggtaac
     4321 aaatccagat actgttatct ctatcaaatc aaagatggga acttctgaaa aagtttctgc
     4381 aaatggtaaa gaatatactc ctcaagaaat ttcagcaatg attcttcaat accttaaagg
     4441 ttatgctgaa gactatcttg gagaaaaagt agaaaaagca gttattactg ttccagctta
     4501 cttcaacgat gcacaacgtc aggcaactaa agacgctggt aaaattgcag gtcttgaagt
     4561 agaacgtatc gttaacgaac caacagcagc cgcacttgct tatggtatgg acaagactga
     4621 caaggatgaa aaaatcttag tttttgacct tggtggtggt acatttgacg tatcaatcct
     4681 tgaattaggt gatggtgtct tcgacgttct tgcaacagca ggtgataaca aacttggtgg
     4741 tgacgacttt gaccagaaaa ttattgattt cttggtagaa gaattcaaga aagaaaatgg
     4801 tattgatctt tctcaagaca aaatggctct tcaacgcttg aaagatgctg ctgaaaaagc
     4861 taaaaaagac ctttcaggtg taactcaaac tcaaatttca ttaccgttca tcactgctgg
     4921 ttctgctggt cctcttcact tggagatgag cttatcacgt gctaaatttg acgatctcac
     4981 tcgtgacctt gttgaacgta cgaaaactcc agttcgtcaa gctctttcag atgcaggctt
     5041 gtcattgtca gaaattgatg aagttatcct cgttggtgga tcaacacgta tcccagcagt
     5101 tgttgaagct gtaaaagctg aaactggtaa agaaccaaat aaatctgtta accctgatga
     5161 agtggttgcc atgggtgctg ctatccaagg tggtgttatc actggggatg tgaaagacgt
     5221 tgtacttctt gacgtaacac cattgtcact tggtattgaa acaatgggtg gtgtcttcac
     5281 taaattgatc gaccgcaaca caactatccc aacatctaaa tcacaagtct tctcaacagc
     5341 agcagacaac caaccagccg ttgatatcca tgttcttcaa ggtgaacgcc caatggcagc
     5401 agataacaaa acactcggtc gcttccaatt gactgatatc ccagctgcac ctcgtggaat
     5461 cccacaaatt gaagtaacat ttgatatcga taaaaatggt attgtatctg ttaaagctaa
     5521 agatctcggt actcaaaaag aacaacacat tgttatccaa tctaattcag gattaactga
     5581 tgaagaaatt gataaaatga tgaaagatgc tgaagcaaat gctgaagcag atgcaaaacg
     5641 taaagaagaa gttgatctta aaaatgaagt tgaccaagcc atctttgcaa cagaaaaaac
     5701 tattaaagaa actgaaggta aaggctttga tacagaacgc gatgcagcgc aatcagcact
     5761 tgatgagttg aaaaaagctc aagaatcagg taaccttgac gacatgaaag ctaaacttga
     5821 agctcttaac gaaaaagcac aagctcttgc agttaaactt tacgaacaag cagctgcagc
     5881 acaacaagca gctcaagggg ctgaaggtgc acaatcagct gattcatcaa gcaagggtga
     5941 tgatgttgta gatggcgaat tcactgagaa ataattatta atattgttca gattcatttg
     6001 aatataagca tgaaaactat actagcatag taaagttctt cgtgataggg gttgctcaat
     6061 aatctagata agtttcagat tacataagct aatttcgcta tcactaaata aaaacatatt
     6121 aataataaat aggcggggcg cctcgctccg tctgttttat taagtgtcat atatatgtta
     6181 actatttaga gctgtaactg ggcaagaata attgttaatc tcttcaagtg tagtatatga
     6241 acaaaatata aaggattaga taatgaacaa tacagaattt tatgatcgtc ttggcgtttc
     6301 aaaagatgct tctcaggacg aaataaaaaa agcttaccgt aggatgtcta agaaatacca
     6361 tccagatatt aataaggaag cgggagcaga ggaaaaatat aaagaggttc aagaagctta
     6421 tgaaaccttg agtgatacac aaaaacgtgc tgcctatgat caatatggcg ctgctggggc
     6481 aaatggcggt tttggtggct tcgatggtgg tggctttggt ggttttgaag acatcttctc
     6541 aagctttttt ggtggaggag ggatgcgcaa tcctaatgca ccacgtcaag gagatgacct
     6601 acaatatcgt gttaatttaa gttttgaaga agctatcttt ggagctgaaa aagaagtatc
     6661 ttataatcgg gagtcatcat gtcatacttg ttcaggaagt ggagctaagc caggtactag
     6721 ccctgtgact tgtcaaaagt gtcatggttc gggtgtgatt aatgttgata cacaaacacc
     6781 tctaggaaca atgcgtcgcc aagtgacttg tgatgtttgt cagggtagtg gtcaagaaat
     6841 caaagagaaa tgtccaacat gtcatggaac tggtcatgag aagaagacgc ataaagtatc
     6901 agttaaaata cctgcaggag ttgaaactgg gcaacaaatt cgtttaacag gacaaggaga
     6961 agctggcttc aatggtggtc cttatggtga tctctttgtt attattaacg ttctcccaag
     7021 ccaacagttt gagcgtaatg gatcaacaat ttattatact ttgaatattg gctttgtcca
     7081 agctgctttg ggtgatacaa ttgatatacc tactgtccat ggagcggtag aaatgtcaat
     7141 tcctgctggt actcagacag gaaaaacgtt ccgtcttcgc ggaaaaggtg cccctaaact
     7201 tcgagggggt ggacaaggtg atcagcatgt tactgttaac atcgtaactc ctactaaatt
     7261 gaatgatgct caaaaagagg cattgcacgc ttttgcagag gcaagtggag ataagatggt
     7321 acatccgaag aagaaaggtt tctttgataa agtcaaagat gctttagatg ttgattaaaa
     7381 aataaaagtc tagaatcgaa agtattctag acttttttgt agttattgct agagggattg
     7441 cttgaaccta aattttacag tacaatgaat ctaatattgt agggggacaa tttttatggt
     7501 aaccaaagtt gaagagattc gctcatattt gatagcttct atacaaaatg gtaagttggc
     7561 tccaggagat cgcctaccat ctataagaca gttagctaat caattttcct gtaacaaaga
     7621 tacagtgcaa cgagttttga tggaattgcg ttttgataat tatatctatg caaagcctag
     7681 gtcaggctat tacgtctttg attctcatca agaggaagtt gaagaagggg ttagtttacc
     7741 aaactctgag attgcaaata tagcttatga tgattttaga ttgtgtttga atgagaccct
     7801 tattggtagg gaagattacc ttttcaatta ttactatcgt caagaaggtc ttcttgattt
     7861 aagtaaagca gtggctaaat taatggaaga aacaggggtc tatgttcccc ttgatgatat
     7921 tgttattacg gctggtactc aacaggcatt atttattttg acacaggtta cctttccaaa
     7981 tcgaaaatct cgagttttaa tagaagaacc gacttatcct cgtatgattg aactaatcaa
     8041 aacacaaaat ttaccctatg aaactattcc tcgaggtact catggaattg attttcagcg
     8101 cttagaggag attttccaga cacaatcaat taagtttttt tatgttatac ctcgcatgca
     8161 taatcctttg ggaacatcct ataatccggt agagatgaaa agattaatag agatggcaga
     8221 gaagtatgat gtttatattg tggaagatga ctatatgtct gattttgcaa gtcagtcacc
     8281 attacattat tatgatactc acgggcgtgt tatttatcta aaatcttttt caaaggctat
     8341 tttccctgct ttaagattag ctgcgatttg tttaccacaa gctttaaaat caacatttat
     8401 ggcttacaag aagttgatgg attatgatac taatctgatt ttacaaaaag cattagcgct
     8461 ttatattgaa aatggccttt atgctaagaa tagtcaatat ttgaaatatc gttatcagaa
     8521 agaccttgca aattcaaaat ctattttagc tgatcaccct aatctacccc catatagttt
     8581 acatcacgat agtgtattat ttgattgttc gaaactcgat aacttcaaaa tattacggca
     8641 atacggcgat actttggaaa attatttttg tcaaaaatcg catcaatctc tcttacaagt
     8701 aaaaaatgat tcctgcttaa agcagttctt gggatcgttg tagtataatt gtagggggac
     8761 agtttaaaat agatattata gttttcataa atgaaaggtg gtaaaataaa cccatgacaa
     8821 ggtataaggc acagatttct tatgatggtt cagctttttc aggcttccaa agacaaccaa
     8881 attgtaggac agttcaagaa gaaattgaga gaactcttaa gcgcctaaat agcgggaacg
     8941 atgtgataat ctatggtgct ggtcgcacag atgctggtgt tcatgcttat ggacaagtta
     9001 ttcattttga tttaccacag gcgcgtgatg tagaaaaatt acgctttggt ttagatacac
     9061 agtgtccaga cgatattgat attgttaaag tagagcaagt gtctgatgat tttcattgtc
     9121 gatatgataa acacattaaa acttatgagt ttttagtaga tatcggtagg ccgaaaaatc
     9181 ctatgatgag aaactatgca acacattacc cttatcctgt cattattgag cttatgcaag
     9241 aagctattaa agatttagta ggcacccatg actttacagg ctttactgct tcaggtacga
     9301 gtgtagaaaa taaagtacgt actatttttg atgcgaaaat tcagtttgaa gcgtctaaaa
     9361 atcttttaat ttttaccttt acaggaaatg gttttttgta taaacaggtg cgtaatatgg
     9421 ttggtaccct gctaaaaatt ggtaatggac gcatgcctat tagccaaatc aaaacaattt
     9481 tacaggctaa aaatcgggat ttagcaggtc caactgcggc aggaaatggt ttatatttga
     9541 aggagattat ttatgaagac gaggaatgtt ttagcaattt caggaaatga tattttctct
     9601 ggcggcggct tgcatgcaga tttagcaact tacgttgtta ataaattaca tggttttgtg
     9661 gcagtaacat gcttaacagc aatgtcagat aaagggtttg aagtcattcc tatagaggca
     9721 tctatactaa agcagcaact agaaagttta aaagatgtag agtttgggtc tattaaacta
     9781 ggattgcttc ccaatgtaga aacagcacaa gttgttttgg aatttgtaaa atcaaaacaa
     9841 gaatgtccag tagtcttaga cccagtctta gtttgtaaag aaaatcatga tttagaagtt
     9901 agccaattac gtgagcagct aattgcgttt ttcccatatg ctgatgtgat tactcctaat
     9961 ttagtagaga ctcaattatt gacgggtctt tcaattgaaa acttagatca aatgaaaatt
    10021 gctgctgaga aactttacga tatgggggct aaacatgttg ttatcaaagg gggcaaccgt
    10081 ttaaatgctg aagaggctac agacctttac tatgatggtg aacgttttga aacctatgtt
    10141 tttcctgtag tagatgccaa taatacaggt gctggatgta catttgcgtc aagtattgcc
    10201 agccaattag ctatgggtaa aaatgtagaa gatgcagtca aaatgtctaa aggtttcgtc
    10261 tatcaagcta ttaaagcatc ggataagtac ggagttgtcc aacattttta ggagatttta
    10321 atgaggaaag agaaaactag tcaattggtt caattggcaa ttgtaacagc tatatctatt
    10381 gtattgggga tgtttataag tattccgaca ccaacaggtt ttttgacatt actagatgct
    10441 ggaatattct ttgctgcctt ttactttggc aaaaaagaag gggctgtggt aggtgcctta
    10501 gctggttttt taattgattt attgaaaggt tatcccaatt ggatgttctt tagtttattg
    10561 attcatgggg cgcaagggta tttagcgggt ttaccaggta gaagacgtct tttaggtctt
    10621 attagtgcaa ctcttgttat ggtattaggt tatgctatag ctagtggatt gatgtatggt
    10681 tggggagctg ttttaccaga tattcctggc aatattatgc aaaatatggt tggtatggtc
    10741 gttggttttg ctcttaataa gtctctagaa agagtgaaga aatgatacta gattataata
    10801 agttaaagca ggaaactaaa gctattgtag ttgatattat tgagcgtagt gctcttaaaa
    10861 aaggtcaaat ttttgtacta ggtctatcat ctagtgaggt ttctggtggt ttaattggaa
    10921 aaaattctag ttctgaaatt ggtgaaatta tcgttgaagt tatcctcaaa gagttacatt
    10981 cacgcggcat ctatttagca gttcaaggtt gtgaacatgt taatagggcg ttggttgttg
    11041 aagcagaatt ggctgaacgt caacagttgg aagtcgttaa tgttgttcct aatctacatg
    11101 ctggcggtag tggtcaggtt gcagctttta agttgatgac atcaccagtt gaggttgaag
    11161 aaattgtggc acatgctgga attgatattg gagatacttc tattggaatg catatcaaga
    11221 gagtacaagt tcccttgatt ccaattagtc gtgagttggg aggggcacac gttacagcac
    11281 tagctagtcg tcctaaatta attggcggtg ctagagcagg atatacgagc gatccaatcc
    11341 gtaaatttta aaataaacct ctgctatgca gaggtttatt gtttatctca acgagagtgg
    11401 ttattggaga aattgtatgt tggtaagtca ataccttctt caagaatagc tttttgataa
    11461 agtttgtaaa attcagcata gatgtcaaac tgagcaccgt tttgcgtgaa gatattaata
    11521 cgataaacaa attgaccatt agcattggtt gtaggaccaa aaacagtagg ctctccaacg
    11581 atagcaggat gttttgaaac atactcttca ttgatttttg ttacaatatc agaaatctga
    11641 tcaaggtttg tatgaacaaa taaaggaata tcaatttgag cacgcatatt gctacgagac
    11701 ttattgctga caattgtaat attacgatta ggaatgaagt gtaaggttcc atcgaaatca
    11761 tgaatttgtg tggtgcgtat cccaacatta gtaacagtgc ctgatacagc accaacatta
    11821 ataatatcac caacatcaaa ttggttttct agcagaataa aaaagccatt gacaacatct
    11881 gataagaagc cttgagcacc aagtccaata gcaaccccag caataccagc cccagctaat
    11941 aaactagaaa ttggaacgcc caatatactt aaaatccaat agaatagaaa gaaataaagg
    12001 gtatagttta aaacattgtg agaaagttta gctaatgttt tttgacgagc tactttttgt
    12061 ctagaaaagg cgagtgagcg attaacagtc ttttcgaaaa gataattgat gacttgccta
    12121 acgataacga atgcaatgat taataataag attgatatta gtttggtgaa gaaagtaaat
    12181 aaaacttcct ctacattaag atggtcaata aatttttcta aagtaatcat agtaatagtc
    12241 tagtaaaaaa aaagccatag agcaagtatt ttaaaagctt atcgttaagc agtttaaagc
    12301 ttcttttttg cataaaaact ttgtaaaacg ttttaaatta tggtaaacta aactgtatct
    12361 aaataatttt aaggagaaat gacttaatgt ctacatcatt tgaaaataaa gctacaaacc
    12421 gtggtattat cacatttaca attagtcaag atgagatcaa gccagctctt gatcaagcat
    12481 tcaacaaagt taaaaaagat ttgaacgttc caggattccg taaaggacat atgcctcgta
    12541 cagttttcaa ccaaaaattt ggtgaagaag cactctacga aaatgcattg aatttagtgt
    12601 tgccaaaggc ttacgaagca gctgttgcag aacttggttt agacgttgtt gctcaaccaa
    12661 aaattgatgt tgtatcaatg gaaaaaggtc aagattggaa gttgacagct gaagttgtta
    12721 caaaaccaga agttaaactt ggggattaca aagacctttc agtagaagtt gatgtttcaa
    12781 aagaagtttc agatgaagaa gttgatgcta aagttgaacg tgagcgtaac aaccttgcag
    12841 aattgactgt taaagatggt gaagcagcac aaggtgatac agttgttatt gactttgtag
    12901 gttcagttga tggtgttgaa tttgatggtg gtaaaggaga taacttctca cttgaacttg
    12961 gttcaggtca atttatccca ggatttgaag aacaattagt aggttctaaa gctggtcaaa
    13021 ctgtagacgt taatgtaaca ttcccagaag attatcaagc agaagatctt gctggtaaag
    13081 atgctaaatt tgttacaaca attcatgaag taaaaactaa agaagttcca gcacttgatg
    13141 atgaacttgc aaaagatatt gatgatgaag ttgaaacact tgatgagttg aaagcgaaat
    13201 atcgcaaaga gctagaatca gcaaaagaaa ttgcatttga tgatgcagtt gaaggtgtag
    13261 caatcgaatt agctgttgcc aatgcagaaa ttgttgaatt accagaagaa atggtccatg
    13321 atgaagtaca tcgtgctatg aatgagttca tgggtaatat gcaacgtcaa ggaatttcac
    13381 cagaaatgta cttccaatta acaggtacaa ctgaagaaga ccttcataag caataccaag
    13441 cagatgctga caaacgcgtt aaaactaacc ttgttattga agcaattgca gctgcagaag
    13501 gctttgaagc aacagatgaa gaaatcgaaa aagaaatcac agatcttgct tcagaatata
    13561 atatggaagc tgaccaagta cgcggtctac tttcagcaga tatgcttaaa catgatattg
    13621 caatgaaaaa agcggttgac gttatcacta gctcagctac tgtaaaataa ttgcttatta
    13681 aattttgaaa agattgggct tcccagtctt tttatttgtg attattcttt tgacgaatag
    13741 ctaaaaatag cgtaaaatag taaatagcga aaatttgaaa atatgactta tctattttct
    13801 atctataaag aatattgcta aacaaaatga tagtcttaca caattaagga gaataacttt
    13861 ggaattagaa gtatttgctg ggcaagaaaa aagtgaactt tcgatgattg aagttgctcg
    13921 cgctatttta gagcaacgtg gtcgtgataa cgaaatgtac ttcagcgatt tggtaaatga
    13981 tattcaaacc taccttggaa aatctgactc agcaatccgt gagtcattgc catttttcta
    14041 ttcagatttg aatacagacg gaagttttat tccacttggc gaaaacaaat ggggacttcg
    14101 ttcatggtat gctatcgatg aaattgacga ggaaatcatc actcttgaag aagatgaaga
    14161 tggcgctcca aaacgtaaga agaaacgtgt caatgctttc atggatggtg atgaagatgc
    14221 tattgattac aatgatgatg atccagaaga tgaagatttc acagaagaaa caccaagttt
    14281 agaatatgat gaagaaaacc ctgacgatga aaaatcagag gtagaatcgt atgattctga
    14341 gattaatgaa atcattcctg atgaagattt agatgaggat gtagaaatca acgaagaaga
    14401 ggaagaagag gaagaagagg aagaagaggt ttagatgggg attttgtatt tgacaaatag
    14461 tcagatatag tttaaactat tattcgggca cctcttatga ggtcagataa agctccctag
    14521 tgataggggg ctattttgtt tttgggaaag atacagaatg gttccagata gttttctgag
    14581 taggcccttg atcagatagt ttctactagt tttgactaga gaagctggta ttatttatca
    14641 atcatgtgtt ttgggtttta cccgtaaact aactgttaga ttaacattta atttaaggag
    14701 tttttaatga caaagtatat ttttgttaca ggtggtgtgg tctcttctat tggtaagggg
    14761 attgttgcag caagtcttgg gcgcttacta aaaaaccgtg ggctaaaggt gacaattcaa
    14821 aaatttgatc catatattaa tattgaccca gggacaatga gcccttatca acatggggaa
    14881 gtttatgtta cagatgatgg tgcagaaact gaccttgacc ttggacacta cgagcgtttt
    14941 attgatatta acttaaacaa gtattcaaat gttacaacgg gtaagattta tagtgaagtt
    15001 cttaaaaaag aacgccgtgg agaatacctt ggtgcaactg ttcaagttat cccacacgtt
    15061 acggatgctt tgaaagaaaa aatcaaacgt gctgcgacaa ctacagattc agatgttatt
    15121 attactgaag ttggcggtac tgttggagat attgagagcc ttccgttttt agaagcactt
    15181 cgtcaaatga aggcagatgt tggttctgat aatgttatgt acattcatac aacgttatta
    15241 ccatacctaa aggctgccgg cgaaatgaaa acaaaaccga ctcaacattc tgttaaagag
    15301 ttacgtggcc taggtatcca acctaatatg cttgttatcc gtacagagca accagcaggt
    15361 caaagtatta agaataaatt ggcacaattt tgtgatgttg caccagaggc tgttatagag
    15421 tcccttgatg ttgaccatat ttatcaaatt ccacttaaca tgcaagctca aaatatggat
    15481 caaattgttt gtgatcactt gaagcttgag acacctgctg ctgatatgac agaatggtca
    15541 gcaatggttg ataaagtaat gaaccttgag aaaaaagtta aaattgcatt ggttggtaaa
    15601 tatgttgaat tacctgatgc ttacctttca gttgtggagg cccttaagca ctcagggtac
    15661 gtcaacgatg ttgctattga cctaaaatgg gtaaatgctg ctgaagtgac agaagataat
    15721 attaaagaac ttgtcggaga tgcagatggt atcatcgttc ctggtggatt tggccaaaga
    15781 ggtagtgagg gtaaaattga agcaattcgc tatgcacgtg aaaatgatgt tccaatgctt
    15841 ggtgtttgtc tcggaatgca gctaacttgt gtcgaatttg cgcgtaatgt tttaaacctt
    15901 catggagcta attcagcaga gcttgaccct aaaacaccgt tcccaatcat tgatatcatg
    15961 cgtgatcaaa tcgatattga agatatgggt ggtacacttc gccttggtct atatccatgt
    16021 aaattgaaag caggttcaag agctgcggca gcttataata atcaagaagt tgtccaacgt
    16081 cgtcaccgtc accgttatga atttaataca aaattccgtg agcaatttga ggctgctgga
    16141 tttgtcttct caggggtatc accggataat cgtttgatgg aagttgttga gttaccagag
    16201 aaaaaattct ttgttgcagc acaataccat ccagaactac aatcacgtcc taaccatgct
    16261 gaagagcttt acacagcttt tgtaacagca gcagttgaaa atatgaaata actttatcgc
    16321 ctcccagtat taggaggcga ttttattttt ttagatagtt tcactatcaa agtatcatta
    16381 gctcttttct ttttctaaaa ataaggtata ataggttgca tgaaaaagat tcgattatca
    16441 aagtttatta aaatgattgt tgttattttg tttttaatta gtgtagcagc tagtttttat
    16501 tttttccacg ttgcccaagt tcgagatgat aaatccttta tttcaaatgg tcaacgtaag
    16561 cctggaaact ctttatatgc ttatgataaa tcctttgata agctattaaa gcaaaaaata
    16621 gaaatgacaa accaaaatat aaagcaagtt gcttggtatg ttcctgctgc taagaaaact
    16681 cataagacag ttgttgtcgt tcatggtttt gcgaatagca aagagaatat gaaggcatat
    16741 ggttggctgt ttcataagtt aggatacaat gttcttatgc ctgacaacat tgcacatggt
    16801 gaaagtcatg ggcagttgat aggctatggc tggaacgacc gcgagaacat tatcaaatgg
    16861 acagaaatga tagtggataa gaatccatca agccaaatta ctttatttgg tgtttcaatg
    16921 ggtggagcaa cagtcatgat ggctagtggt gaaaaattac ctagtcaggt tgttaatatc
    16981 attgaagatt gtggttattc tagtgtttgg gatgaattaa aatttcaggc taaagagatg
    17041 tatggtttac cagccttccc actcttatat gaagtttcaa caatttctaa aatcagagca
    17101 ggtttttcgt atggacaagc aagtagtgtc gaacaattga aaaagaataa tttaccagcc
    17161 ctctttattc atggtgataa ggataatttt gttccaacaa gtatggttta tgacaactat
    17221 aaagctacag caggtaagaa agagctttat attgtaaaag gggcaaaaca tgcgaaatct
    17281 tttgaaacag agccagaaaa atatgagaaa cgtatctcta gttttttgaa aaaatatgaa
    17341 aaataatatt gacaacaata tgataagttg atataattta ttatgttgtt aagcggggat
    17401 ggcggaattg gcagacgcgc aagactaagg atcttgtgac cgcttttggt cgtgagggtt
    17461 caagtccctc tctccgcaga ctagtcgagt tcatttggaa ctcgactttt tgcatattta
    17521 agataaagat ggagtatcac atgtcaaaag tacgtggctt tgaattagtt agccaattta
    17581 gcaataaaga gttattacca aaacgagaaa cagctcacgc agctggctat gatttaaaag
    17641 tagctaaaaa aacggttatt gaacctggtg aaattacatt ggtcccgacc ggtattaaag
    17701 cacacatgca accaggggag gtcctgtacc tttatgatcg ttcatcgaat ccacgtaaaa
    17761 aagggattgt cctaataaat tcagttggtg ttattgatgg tgactactat aataaccaag
    17821 tcaatgaagg acatattttt gcacagatgc aaaatattac tgatcaggct gttattttag
    17881 aagaagggga acggattgtt caggctgttt ttgccccatt cttattggca gatgacgatc
    17941 aggctacagg aatgcgtaca ggtggttttg gtagtactgg aaaataaaat tattttacga
    18001 ataatgttag aaaaggctag acttacttct tagccttttt tgatgagagt aaacttatat
    18061 tttctctaac ttggtgtgat ataatgagtg tactagttat ttggtaccaa gctatcatat
    18121 taatttagaa gttataaagg aggcctgagt ggctaagaaa aaatcagtct tcacctgtca
    18181 agagtgtggt taccaatcac ctaagtattt gggacgttgt ccaaattgct cagcctggtc
    18241 ttcatttgta gaagaggttg aagttcaaga agttaaaaat gcacgagtta gtttgaacgg
    18301 tgaaaagtcc cgaccgacga agttaaaaga tgtttcctca attaactatt ctagaactaa
    18361 gactgatatg gatgaattta accgtgttct aggaggcggt gttgtaccag gaagtttggt
    18421 tctgattgga ggagaccctg ggataggtaa atctacttta ttactacaag tatcaactca
    18481 gctagctaat aaaggtacag tattatatgt ctcaggtgag gaatctgcag aacagattaa
    18541 gttacgaagt gagcgtctag gcgatatcga taatgaattt tacctttatg ccgagactaa
    18601 tatgcaaagt attcggtcgg agattgagaa gattaaacca gattttctta ttattgattc
    18661 tatacaaact attatgagtc ccgaagtatc aagtgtccaa ggctctgtta gccaagttcg
    18721 cgaagtgacc gctgagttga tgcagttagc taaaactaat aatattgcta cttttattgt
    18781 aggtcatgtg actaaggagg gaacattagc aggccctcga atgctagaac atatggtgga
    18841 tacggttctt tattttgaag gggagcgtca tcacacgttc cgtattttac gggcagtcaa
    18901 aaaccgtttt ggttcgacca atgaaatagg tatctttgaa atgcagtcgg gtggtctagt
    18961 tgaagttttg aatccaagtc aagtttttct tgaagaacgt ttagacggtg caacaggatc
    19021 tgctattgtt gttactatgg aaggaacaag gcctatttta gcagaggttc aagctttagt
    19081 gactccaact gtttttggaa atgctaagcg cacaaccaca ggtcttgact ttaatcgtgt
    19141 cagtcttatt atggcagttc tagaaaaacg ttgtggttta ttgcttcaaa atcaagatgc
    19201 ctatttaaaa tcagcgggtg gtgttaaatt ggatgaacca gcaattgatt tagctgttgc
    19261 tgtagctatt gcttccagtt acaaagaaaa acccacaaat ccacaagaat catttattgg
    19321 agaaattgga ctcactggtg agattcgtcg cgtgacacgt attgagcaac gtattaatga
    19381 ggctagtaaa ttgggcttta ctaaaattta cgcaccaaag aattctctag ctgggattga
    19441 aatacctaaa ggcattgatg ttattggtgt gacaactgtt agtcaggtct taaaagcagt
    19501 gttttcaaca taatgattag attcggttca gccgaatctt ttttggtggc gagagacatt
    19561 tcgtgaaaaa taatgggcaa aatgataata taaaagggtt ggtatttagt aaatatcgaa
    19621 tgatgttaca ctaacaaagg agatctctat gaccacttat tttgataact ttttaaaaac
    19681 caatcaagcc tatgctgact tacatggaac agctcatcta cctatcaagc caaagacaaa
    19741 ggttgctatt gtaacctgta tggattctcg tttacacgtt gctcaagctc tagggctagc
    19801 tttaggagat gctcatattt tacgtaatgc tggtggacga gttacagccg atgttctacg
    19861 ttctttagtt atttcgcaac aacaattagg aacacgagaa atcgttgttc tacaccatac
    19921 agattgtgga gcacagacgt ttactaacga agcttttgct gctcaattac agagagattt
    19981 gggtgttgat atgcatggac atgattttct tccttttaat gatattgaag aaagcgttcg
    20041 tgaagatgtg gcaaaattac atgcttctcc attaatccca aatgatgttg ttatttctgg
    20101 tgctatttat gatgtagata ctggtcgtat ggtggaagtt aaatagaaaa aatcctgtcc
    20161 ttagcgatgg gtttttttat tttagaaaac tagatttaaa ggtaacagtt aggcttatca
    20221 tgccttatgc tggaattcag acagaggaaa catcttttgc tatactgaaa ctatattata
    20281 aaagcggagg aaatttaaaa tgaaaaaata tgatgtcatt gttcttggtt ttggtaaagc
    20341 aggtaaaaca ttagcagcaa aattagcaac acaaggtaaa agcgttgcaa tggtcgaaga
    20401 agacgataaa atgtatgggg gaacatgtat caacattgga tgtattccga caaaaacgct
    20461 tttagtatca gctagcaaaa atcacgattt tcaagaagct atgactacta gaaatgaagt
    20521 tacaagtcgt ttacgtgcta aaaattttgc tatgttggat aataaagaca ctgtggatgt
    20581 ttataatgct aaagctcgct ttatatcaaa taaagtggtc gaattaacag gaggagctga
    20641 caaacaagaa cttacagctg atgttattat tatcaatact ggtgctaagt cagtacaatt
    20701 accaattcca ggattagctg attctcaaca tgtttatgat tcaacggcaa tccaagaatt
    20761 ggctcatctt cctaaacggt taggtatcat tggaggcggt aatattgggc ttgaatttgc
    20821 gactttatat tccgaattag ggagcaaagt gacagttatt gatagtcaat ctcgtatctt
    20881 tgcgcgtgag gaagaggaat tatcagagat ggctcaagat tatttagaag agatggggat
    20941 atcttttaaa ttatcagccg atatcaaatc tgtgcaaaat gaagatgaaa acgttgttat
    21001 tagctttgag gatgaaaaat taagctttga tgcagtatta tatgcaacag gacgtaagcc
    21061 aaatacagaa ggtttagcac ttgaaaatac agatattaaa ttgacagaac gtggagctat
    21121 tgctgttgat gaatattgcc aaacttctgt tgagaatatt tttgcagttg gtgatgttaa
    21181 tggcggccct caattcacat atatttcttt agatgactca cgtattgtct taaattattt
    21241 gaatggtgat aaggattatt ctctaaaaaa tcgaggagca gtaccgactt caacctttac
    21301 aaatcctcct ttagcaacgg taggtctaga tgaaaagacc gctaaggaaa aaggatatca
    21361 agttaaatct aatagccttc ttgtttctgc catgccacgt gcacatgtta ataatgacct
    21421 tagaggtatt tttaaagttg tggtagatac tgagacaaat cttattttgg gagctcgctt
    21481 atttggtgca gaatcccatg aattaattaa tatcatcaca atggcaatgg ataataaaat
    21541 cccatatact tatttccaaa aacaaatttt cactcaccca acaatggtag agaattttaa
    21601 tgatttgttt aacttttaag aatattttta aaaagtaagg caattttgct ttactttttt
    21661 tatc
//
LOCUS       DATRUK010000031        20399 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.031, whole genome shotgun sequence.
ACCESSION   DATRUK010000031 DATRUK010000000
VERSION     DATRUK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20399
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.031"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(99..380)
                     /locus_tag="VBE34_001141"
     CDS             complement(99..380)
                     /locus_tag="VBE34_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263863.1"
                     /translation="MSLIGAVLKGTKEDKEFKKQIQKALVRFVHAPEIVEEADSIKTT
                     YSEKIDDISETAKSSIKTIKNQIDTGLVGQFWEKVKAQVDLNKGSYDDL"
     gene            complement(414..800)
                     /locus_tag="VBE34_001142"
     CDS             complement(414..800)
                     /locus_tag="VBE34_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263864.1"
                     /translation="MVTILDQNENKINSLKINLGKLEDNYHRENMEIEDKLYSLEDKK
                     NELETYLQEVYEETSHFLRQNDSDGSTFMSLNRIIESYQMELIDEYENYKQTIIVQEE
                     NSRTQFLKDRVKLENDISSLEGRKYY"
     gene            complement(785..1465)
                     /locus_tag="VBE34_001143"
     CDS             complement(785..1465)
                     /locus_tag="VBE34_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263865.1"
                     /translation="MAKHPEYFANFRHKEDNVTWWNDFNKLDDKGYGTVKWVNGKSHK
                     IESWKFTDDGQLKDEKGNIVNPKSPAVQSVLYEEVHFQKAKAKLKKSGGKLSHSEKVY
                     LDSEQAIFIANGLTTASQTASDDIKKNAELAKEKASELFAKTKVMPPGITDLSPEELA
                     DAYSAGGVREDTIVTPIETFFDEKVTNAQEITTSYTNLQKQIESGVQKLLEEDSKLAG
                     EFKEWSQY"
     gene            complement(1411..1998)
                     /locus_tag="VBE34_001144"
     CDS             complement(1411..1998)
                     /locus_tag="VBE34_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263866.1"
                     /translation="MTYSYSEYDLNRLNQGENVYSVNPNYVKQNGGKIITANPKKSNE
                     TNLIVTKTGKAFKVLKTSPDDMSGYQGMAVAPIIKGKVDYNSVAVISAATDSSNYKDL
                     IGAVSSAQPHQSSTQLKSADKFLKDVQSHDKWTVTQLSGYSQSAYMLKLGAKYHIPTT
                     VFNGWFRYSTLNEDEKNSWLSILNILLIFDIKRIM"
     gene            2407..2721
                     /locus_tag="VBE34_001145"
     CDS             2407..2721
                     /locus_tag="VBE34_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO7263867.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            2737..3123
                     /locus_tag="VBE34_001146"
     CDS             2737..3123
                     /locus_tag="VBE34_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO7263868.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            3152..4537
                     /locus_tag="VBE34_001147"
     CDS             3152..4537
                     /locus_tag="VBE34_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO7263869.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            4540..4692
                     /locus_tag="VBE34_001148"
     CDS             4540..4692
                     /locus_tag="VBE34_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7263870.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            4715..5920
                     /locus_tag="VBE34_001149"
     CDS             4715..5920
                     /locus_tag="VBE34_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO7263871.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            5963..6184
                     /locus_tag="VBE34_001150"
     CDS             5963..6184
                     /locus_tag="VBE34_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263872.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            6301..6798
                     /locus_tag="VBE34_001151"
     CDS             6301..6798
                     /locus_tag="VBE34_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEO7263873.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            6773..7279
                     /locus_tag="VBE34_001152"
     CDS             6773..7279
                     /locus_tag="VBE34_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7263874.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            7263..9710
                     /locus_tag="VBE34_001153"
     CDS             7263..9710
                     /locus_tag="VBE34_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7263875.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            9713..11890
                     /locus_tag="VBE34_001154"
     CDS             9713..11890
                     /locus_tag="VBE34_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO7263876.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            11887..12888
                     /locus_tag="VBE34_001155"
     CDS             11887..12888
                     /locus_tag="VBE34_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEO7263877.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            12885..13820
                     /locus_tag="VBE34_001156"
     CDS             12885..13820
                     /locus_tag="VBE34_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7263878.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            14065..14181
                     /locus_tag="VBE34_001157"
     CDS             14065..14181
                     /locus_tag="VBE34_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO7263879.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14197..16116
                     /gene="tet(M)"
                     /locus_tag="VBE34_001158"
     CDS             14197..16116
                     /gene="tet(M)"
                     /locus_tag="VBE34_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO7263880.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTD
                     NTLLERQRGITIQTAITSFQWKNTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKIGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     HPNMRVMNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSILGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTSFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            16235..16402
                     /locus_tag="VBE34_001159"
     CDS             16235..16402
                     /locus_tag="VBE34_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO7263881.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(16462..16815)
                     /locus_tag="VBE34_001160"
     CDS             complement(16462..16815)
                     /locus_tag="VBE34_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7263882.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            17320..17742
                     /locus_tag="VBE34_001161"
     CDS             17320..17742
                     /locus_tag="VBE34_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7263883.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            17739..17969
                     /locus_tag="VBE34_001162"
     CDS             17739..17969
                     /locus_tag="VBE34_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7263884.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            18430..18633
                     /locus_tag="VBE34_001163"
     CDS             18430..18633
                     /locus_tag="VBE34_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO7263885.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            18715..19932
                     /locus_tag="VBE34_001164"
     CDS             18715..19932
                     /locus_tag="VBE34_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO7263886.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(20054..>20399)
                     /locus_tag="VBE34_001165"
     CDS             complement(20054..>20399)
                     /locus_tag="VBE34_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263887.1"
                     /translation="LIFSGGFYMKEQQRKEELKRNREYEVSLVKALKNSYSDIKEIQI
                     SNPITTTMPGDWRSTVKIIFSDNDEITYCITHNKDDEINRSGSINEKEHLFLSKTKYK
                     FLIIFLYFLKCS"
BASE COUNT         6556 a         3686 c         4060 g         6097 t
ORIGIN      
        1 tccttcatgt aaaatgttat gtcttctaat ccaacttgat tattttcata tctataataa
       61 ctatcaggat agtaagcttc ctcaacattt tcactcgatt ataaatcatc ataactacct
      121 ttatttaaat ctacctgtgc ctttactttt tcccaaaatt gtcctacaag tccagtgtca
      181 atttggtttt ttatcgtttt tattgatgat ttagcagtct cagaaatatc atcaatcttt
      241 tcactataag tcgtcttaat agaatcagct tcttctacta tttccggggc atggacaaac
      301 cttactaaag ctttttgaat ttgctttttg aattctttgt cttctttggt tcctttcaat
      361 acagcgccta ttaaactcat ttattgcctc tttcacttta tagtccatct aaattagtag
      421 tattttcttc cttctaaact agaaatgtca ttttctaatt ttactcggtc ttttagaaac
      481 tgtgtccgac tattttcctc ttggacaatg atagtctgtt tatagttttc atattcatca
      541 attaattcca tttgataact ttcaattatt cgatttaaac tcataaaagt cgaaccatct
      601 gaatcatttt gtctcaaaaa gtgacttgtc tcttcataga cttcttgtaa ataagtctct
      661 aactcgttct ttttatcctc aaggctatat aatttatctt ctatctccat attttcgcga
      721 tgataattat cttccaattt ccctaggttt atttttagac tattaatttt gttttcattt
      781 tgatctagta ttgtgaccat tccttaaatt ctcctgctaa tttggagtcc tcttctagca
      841 atttttggac accagattca atttgttttt gaagattagt atatgaagtt gtgatctctt
      901 gtgcattagt aactttttca tcaaagaaag tttcaattgg tgttactatg gtgtcctctc
      961 taactccacc cgcactatat gcatcagcta attcttctgg acttaaatct gttataccag
     1021 gaggcatcac tttagttttt gcaaatagtt ctgatgcttt ttctttagct aattcagcat
     1081 tcttcttgat atcatcagaa gctgtttgag atgccgtggt taaaccgtta gcaataaata
     1141 tagcctgttc agaatctaaa tatacttttt cactatgact aagtttgcct ccagattttt
     1201 ttaatttagc tttagctttt tgaaaatgaa cttcttcata aagaacagac tgaacagctg
     1261 gtgacttagg atttacgatg ttaccttttt catcttttaa ctgaccatca tcagtaaatt
     1321 tccaactctc tattttatga cttttaccat ttacccattt cacggtacca taacctttat
     1381 catctagttt attaaaatcg ttccaccaag ttacattatc ctctttatgt cgaaaattag
     1441 caaaatattc aggatgctta gccatgaatt tttttcatct tcatttaaag tgctatacct
     1501 aaaccatcca ttaaatactg tagtcgggat atgatattta gcacctaatt ttagcatata
     1561 agcagattga gaatagcctg aaagctgagt cacagtccat ttgtcatgtg actgtacgtc
     1621 ttttaaaaat ttatcagctg attttaactg agtcgaactt tgatgtggtt gagcagatga
     1681 aactgcgcca attaaatctt tataattact tgaatccgtt gcggcggaaa taactgcaac
     1741 actgttataa tctactttac ccttgataat tggtgcaact gccataccct gatagccaga
     1801 catatcgtca ggagaagttt ttaatacctt aaacgcttta cccgtctttg ttactattaa
     1861 atttgtttca ttagattttt ttggattagc tgttattatt tttccgccat tttgtttcac
     1921 atagtttgga tttacactat atacgttctc accttgattc aatctattta aatcatactc
     1981 gctataagaa tatgtcatta tcttttttcc ttttctcctg tagaaaactc aactaaaata
     2041 tgattttttg ttcttccata tttttaattt aaaaatagca taaaaatcta gttatccgca
     2101 taaaaactgg acttatcaca ctttatcaag gtcaaaacca ctcaatttac tactaattta
     2161 ctacttatga atgagctttg atacgacgat ttatccttga aaagtgaaga tataaagata
     2221 cttccaataa aatttgaata tttaataggt agacacttca aaaaatgagg tgtctatttt
     2281 tttacccgat tttgaaagga agtgaactta tgaaaacaaa aaatcaagaa tcaaaaggtc
     2341 gttccccact ctttaagacc atcaaacatt cattcagcca ataaaaaaga aaggataggt
     2401 aaaaatatgg aacttaaatt tgtgattccc aacatggaaa aaacattcgg caatttagaa
     2461 tttgctggcg aggataaagt cgttcagcga agaatcaacg gacggctaac tgtcttatca
     2521 agaagctata atctctattc tgatgttcaa agagcagatg atattgtggt ggtgcttcct
     2581 gctgaagctg gcgaaaaaca tttcggcttt gaggaacgtg tgaagttagt caatccacgt
     2641 attaccgcag agggctacaa aatcggcact cgtggtttta caaattacct tttacatgct
     2701 gacgacatga taaaagaata aagaaagaga ggaaaaatga tgagattagc aaatggcatt
     2761 gtattagata aagacacgac ttttggagaa ttgaaattct ctgctctacg tcgtgaagtg
     2821 agaatccaaa atgaagacgg gtcggtttca gatgaaatca aggaacgtac ctatgactta
     2881 aaatccaaag gacaaggacg catgattcaa gtaagtattc ctgccagcgt gcctttgaaa
     2941 gagtttgatt ataacgcacg ggtggaactt atcaatccca ttgcggacac cgttgctact
     3001 gccacctatc aaggagcaga tgttgactgg tatatcaagg cagacgatat tgtgctgaca
     3061 aaggattcta gttcattcaa agctcaacca caagcaaaga aagaaccgac acaagacaaa
     3121 tagtcgctag gtagaaagga gactttttcg catgaaacag cgtggtaaaa ggattcgccc
     3181 atctggtaaa gatttagtct ttcattttac gatagcgtca ctcctgcctg ttttcctgct
     3241 ggttgtcgga ctgtttcatg tgaagacaat ccagcagatc aactggcagg attttaacct
     3301 atcacaagca gataagattg acattcccta tttaattatc agtttcagtg tcgcaattct
     3361 tatctgcttg ctggtagcgt ttgtattcaa acgggttcgc tatgatacgg ttaaacaact
     3421 ttaccaccgt caaaaactgg caaagatgat acttgaaaac aagtggtatg aatctgaaca
     3481 ggtcaaaaca gagggtttct ttaaagatag tgctggtcgt acaaaggaaa agataaccta
     3541 cttccctaaa atgtattatc gacttaaaaa tggcttgata cagatacggg tggaaatcac
     3601 gctgggaaaa tatcaagacc aactcttaca cttggaaaag aaattagaga gtggcttgta
     3661 ctgtgagctg acggataaag agttaaagga ttcctatgtg gaatatactt tgctctatga
     3721 caccatagcc agtcgtattt ctattgatga agtagaagct aaagatggta aacttcgctt
     3781 aatgaaaaac gtatggtggg aatatgataa gctccctcat atgttgattg ctggtggtac
     3841 aggtggcggt aaaacttact ttatactgac actgattgaa gccttgcttc atacagattc
     3901 aaaactgtat attcttgacc cgaaaaatgc tgaccttgcg gacttaggtt ctgtgatggc
     3961 aaatgtctac tatagaaaag aagacttgct ttcttgcatt gaaacattct atgaagaaat
     4021 gatgaaacgt agtgaggaaa tgaagcagat gaagaactat aagactggca aaaattatgc
     4081 ttacttaggt ctcccggcac acttcttaat ctttgatgaa tacgtcgctt tcatggaaat
     4141 gctgggaaca aaagaaaaca ccgcagttat gaataagctg aaacagattg tcatgttagg
     4201 tcgtcaagct ggcttctttc taatactggc ttgtcaacgt ccagacgcaa aatatttagg
     4261 cgacggaatc cgtgatcagt ttaatttcag agtggcttta ggtcgtatgt ctgaaatggg
     4321 ctatggcatg atgtttggca gtgacgtaca aaaggatttc ttcttaaagc gaatcaaagg
     4381 tcgtggctat gttgatgtag gaacaagtgt catatcagag ttttatactc cccttgtacc
     4441 aaaaggatat gatttcttgg aggaaattaa aaagttatcc aacagcagac agtccacgca
     4501 ggcgacgtgc gaagcggaag tcgcaggtgt ggactgatct tgctggctgg tgtggcaata
     4561 gccacgccag cacttaaccc cccgtatcta acaggggggt acaaatcgac aggaaacagt
     4621 caaaaaaaca ttagaaaatc ctttggttac aagggattta caaaatttca gcgtatgtca
     4681 aatgggcttt aaaagttgac atacgccttt ttgattggag ggatttttac tgaatgaaca
     4741 aacttggtta cagcatttaa aagaaaaacg cttggcttat ggactatctc aaaaccgttt
     4801 agctgttgcg actggtatta caaggcagta tctaagcgat attgaaacag gaaaagtcaa
     4861 gccatcagag gatttacagc agtccctttg ggaagctctg gaacgcttca atcccgacgc
     4921 tccccttgaa atgctgtttg attatgtaag gattcgcttt ccgacaacag acgtacagca
     4981 ggtggtcgaa aacatcttac aactgaaact gtcctatttt cttcatgagg actatggttt
     5041 ctattcttat tcagagcatt atgctttagg cgacatattc gtcctttgct cccatgaact
     5101 ggacaaagga gttctggtgg aattgaaagg tcgtgggtgc agacaatttg aaagctatct
     5161 tctggcacaa caaagaagct ggtatgagtt ctttatggac gttttggtgg ctggcggtgt
     5221 gatgaaacgc cttgaccttg ccattaacga taagacaggg attttgaata tccctgtact
     5281 cactgaaaag tgccaacagg aagaatgtat ctccgtcttc cgcagtttta aaagctatcg
     5341 cagtggcgaa ctggtacgca aagaggaaaa ggaatgtatg ggaaacaccc tctatatcgg
     5401 ttcattacaa agtgaagttt atttctgtat ctatgaaaag gactacgagc agtacaagaa
     5461 aaatgatatt cccattgaag acgcagaagt aaaaaaccgt tttgagattc gattgaaaaa
     5521 tgagcgtgcc tattatgcag tccgtgattt actcgtctat gacaatccag agcataccgc
     5581 ctttaaaatt atcaatcggt atatccgttt tgtagataaa gacgattcca aacctcgttc
     5641 tgattggaaa ctgaatgaag aatgggcttg gtttattggg aacaatcgtg aacgattaaa
     5701 actaaccaca aaaccagagc cttactcctt ccaaaggacg ctgaactggc tatctcatca
     5761 agttgccccg accttaaagg ttgcgattaa acttgatgaa atcaaccaga cgcaggttgt
     5821 aaaagacatt ctcgaccatg cgaaactgac agaccgacac aagcagattt tgaagcaaca
     5881 gtcagtaaaa gaacaggacg tgataacaac aaaaaaataa ctcaaataca aattcattga
     5941 atatagagag gagaacattt ttatgaattt tggacaaaac ctttataact ggtttctatc
     6001 aaacgctcaa tcactggtgc ttttagcaat cgttgtgatt ggcttgtatc ttggcttcaa
     6061 gcgtgagttt agcaaactga ttggcttttt aattattgcg attattgcgg ttggcttagt
     6121 cttcaacgct gctggagtaa aagacatttt actagagcta ttcaatcgca ttattggtgc
     6181 ttaaataaaa ccgttctttt gtggaatata agtggttttc ttatgttccg caaaggaatg
     6241 gtacaccaaa cgaagtgcgg tagggatttt tgaatctcta caaagaaagg acgtgaatat
     6301 atggacgata tgcaagtcta tattgcgaat ttaggcaaat acaatgaggg cgaattggtc
     6361 ggtgcgtggt ttacctttcc cattgacttt gaggaagtca aagagaaaat cggcttgaat
     6421 gatgaatatg aggaatacgc cattcatgac tacgagttac cctttacggt tgacgaatac
     6481 acttccattg gcgaactcaa tcgactatgg gaaatggtat cggaattacc cgaagaatta
     6541 caatcggagc tatctgctct gctcactcat ttttcaagca ttgaagaact aagcgaacat
     6601 caagaggata ttatcattca ttccgattgt gatgatatgt atgacgtggc acgctactac
     6661 attgaagaaa cgggtgcttt aggcgaagta ccagctagtc ttcaaaacta tattgattat
     6721 caagcctatg gtcgggattt agacctttca ggaacgttta tctcaaccaa tcatgggatt
     6781 tttgaaatcg tctattaaat ctgtcggtac attactactg gcagattttc tattttacgg
     6841 ggtggctcaa tcagctaccc ctatttttta tgaaaggatt gattacatga agaaaatacg
     6901 aagctatacc agtatctggt ctgtggaaaa ggtactgtat tctatcaatg attttagact
     6961 tccgtttccc ataaccttta cgcaaatgac atggtttgtc gtgtcactct ttgcagtgat
     7021 gatacttggc aacttgcccc ctctttccat gatagaggga gcatttctca aatactttgg
     7081 gattcctgtg gctttcacat ggtttatgtc tacaaaaact tttgatggta aaaagcctta
     7141 tggatttttg aagtctgtca ttgcttatgc actgcgacca aagctgacct atgcaggaaa
     7201 aaaagtaacg cttggcagaa accagccaca agaagccatt acagcagtta ggagtgaatt
     7261 ttatggcata tccaattaaa tacattgaaa acaatctcgt ctggaataaa gacggggaat
     7321 gttatgctta ctatgagctt gttccttaca attactcatt tctaagtcca gaacagaaaa
     7381 tacaagtgca tgattctttc agacagctta tcgcacaaaa tcgtgatggc aaaattcatg
     7441 ctttacaaat cagtacagaa tccagcatac gttctgcaca agagcgttcc aaaaatgaag
     7501 tcactggcaa gctcaaagcg gttgcctatg acaaaatcga ccaacagaca gacgctttaa
     7561 tatccatgat tggcgaaaat caagtgaact accgtttctt tatcggcttt aagttgcttc
     7621 tcaacgatca ggagttttct atgaaaagtc ttaccgttga agcaaaaaat gctttgtctg
     7681 attttgtcta tgatgtgaac cataagctga tgggcgattt tgttagtatg agtaatgatg
     7741 aaatcctgcg ttttcagaag atggaaaagc tcttagaaaa taaaatctct cgtcgtttca
     7801 aaatccgcag gttagataag gacgacttcg gctatctgat tgaacacctt tacggacaga
     7861 caggcactgc ctatgaagag tatgagtacc atctatcaaa gaaaaagctg gataatgaaa
     7921 cgctgattaa atactatgac ttgattaagc ctactcgctg tttggtggaa gaaaaacagc
     7981 gatatttgaa aatccagcag gaagatgaaa ccgtctatgt agcttacttt accattaaca
     8041 gcattgtcgg agaactggac ttcccgtcct ctgaaatctt ctactaccag caacagcaat
     8101 ttacattccc gattgatacg tcaatgaatg tggaaattgt agcgaatcgt aaagccctat
     8161 ctactgtccg caataaaaag aaagaactga aagacttgga taaccacgct tggcaaagtg
     8221 ataatgaaac cagctccaat gtggcggaag ctctggaaag tgtgaatgag ctggaaacca
     8281 atttagacca aagcaaggaa tctatgtaca agctgtctta tgtggtaagg gtatcagcaa
     8341 atgatcttga cgaactcaaa cgtcgttgta atgaagtgaa agatttttat gacgatttaa
     8401 gcgtaaaact ggtacgacca tttggggata tgctcggctt acatgaagaa tttttacctg
     8461 ccagcaagcg ttatatgaat gattatattc aatacgtgac ctctgatttc ctcgctggtt
     8521 taggttttgg tgctactcaa atgctggggg aaaatgaggg gatttatgtt ggctacagct
     8581 tagatactgg acgcaatgtc tatctgaaac ctgctcttgc cagtcaaggg gttaagggtt
     8641 cagtaaccaa tgcgttagcg tcggcttttg ttggttcgct gggtggtggt aaatcctttg
     8701 cgaataacct tatcgtctat tatgcggtgc tttatggggc acaagcagtg attgtagacc
     8761 caaaagcaga acgtggcaga tggaaagaaa ccttgccaga gatttcccat gaaatcaata
     8821 tcgtcactct gacttctgat gagaaaaaca aaggcttact tgacccttat gtgattatga
     8881 aaaatcccaa agattctgaa tcactggcta ttgatattct gacattcctt acggggattt
     8941 cctctcgtga tggggaacgc ttcccaatcc ttagaaaagc cattcgtgca gtaaccaata
     9001 gtgaagtacg agggttgatg aaagtgattg aggaattacg ggttgagaat acgccactaa
     9061 gtaccagtat agccgaccat atcgaaagtt ttacagacta tgactttgca catttattat
     9121 tcagtaatgg ttatgtggag cagtctatca gcttagaaaa acaactgaac attatacagg
     9181 ttgcggactt ggtacttccc gacaaggaaa cttcctttga ggaatatacc actatggagc
     9241 ttttatccgt tgctatgctg attgtcatta gtacctttgc tttagacttt atccatacag
     9301 accgaagcat tttcaagatt gtagatttag acgaagcatg gagcttttta caggtagcac
     9361 aaggaaaaac actatctatg aagctggttc gggctggtcg tgctatgaac gctggggtat
     9421 atttcgtgac ccaaaataca gacgacctct tagatgaaaa actgaaaaat aacctcggct
     9481 taaaatttgc atttcgttcc actgacctta acgagattaa aaagacctta gccttttttg
     9541 gtgtagaccc agaggacgaa aacaatcaga agcgattgcg tgatttggaa aacgggcaat
     9601 gccttatcag tgatttatat ggtcgtgtcg gtgtgataca gttccaccct gtatttgaag
     9661 aactgctcca tgcctttgat accagaccac ctgtgcgaaa agaggtgtaa atgtgaaacc
     9721 atcaatagta aacagaataa aatcaaactg gacgctgaaa cgtctaggta aagtggcaat
     9781 gacagtggct ttcacacttg tgattgccat ttttctttta gccatgctgg gaacggtggt
     9841 tcaagctgcg ggcttggtag atgatacggt caatgtggca aatgaataca gccgataccc
     9901 acttgaaaac tatcaactgg atttttatgt ggataatagc tggggctggc ttccgtggaa
     9961 ctggtcggac gggattggaa aacaggtcat gtatggacta tatgccatta ccaattttat
    10021 ttggacaatc agtttgtatg tttccaatgc gacaggttac ttagtacagg aagcctattc
    10081 cttagacttc atttccgcta cagcagattc cattggtaag aatatgcaga ccttagctgg
    10141 tgtgagtgca aacggatttt caacagaggg tttctatgtt ggattcctct tactcttgat
    10201 tttggttctt ggggtttatg ttgcctatac gggactgata aagagagaaa ccacaaaggc
    10261 aattcatgcc attatgaatt ttgtgctggt gtttatccta tcggcttcct ttattgccta
    10321 cgctcccgac tacattaaaa aaatcaatga cttttcatca gacatcagta atgccagttt
    10381 atcacttggc acgaagattg tcatgcccca ttccgatagt caaggcaagg acagcgtgga
    10441 cttaatcaga gatagcctgt tttccataca ggttcagcaa ccgtggctac tgcttcaata
    10501 caacagttca gacattgaaa gtatcggtat tgaccgtgtg gaaagcctgc tctccaccag
    10561 cccagattcc aacaatggcg aagacagaga aaaaattgtt gcggaagaaa ttgaagacag
    10621 aagcaatacc aatctaacca ttacaaagac cattaaccgt ttaggtacag tcttcttcct
    10681 atttgtcttc aatattggga tttccatatt tgtattccta ttaacaggaa tcatgatttt
    10741 ctcgcaggta ctttttatca tctatgctat gtttctgcct gtgagcttta ttttaagcat
    10801 gattccatca tttgatggta tgtcaaaacg agccataaca aagctcttta ataccatttt
    10861 gacacgagct ggaatcacat tgattattac gacagcattt agtatttcaa ccatgctcta
    10921 taccttatcg gctggttatc cgttcttttt gattgctttt ctacagattg tgacctttgc
    10981 aggaatctac ttcaagctgg gcgatttaat gagtatgttt tctctacaga gtaacgattc
    11041 tcaaagtgtg ggaagtcgtg tgatgagaaa acctcgtatg cttatgcacg ctcacatgca
    11101 ccgtctacag cggaaacttg gacgttccat gactactcta ggggctgggt ctgccattgt
    11161 tacaggtaaa aaaggacagt cgggttcggg gagttctgca aggacacaag cagatcactc
    11221 ccgaccagac ggaaaggaaa aatcaacact tggaaaacgt atcggtcaaa ccatcggtac
    11281 agtagctgat accaaagaca gaatggtaga cactgctagt ggtttgaaag aacaggttaa
    11341 agatttgccg accaatgcaa gatatgcagt atatcaagga aaatccaaag taaaagagaa
    11401 tgtccgtgat ttaaccagta gtatttctca aaccaaagcg gacagagcca gtggacgcaa
    11461 ggaacagcag gaacaaaggc gaaaaaccat tgcgaagcgt cgctctgaaa tggaacaggt
    11521 caaacagaaa aaacagcctg cttcttctgt tcatgaaaga ccgactacaa gacaagaaca
    11581 atatcatgat gaacagacct caaaacagtc taatattcag acttcatata aggaatctca
    11641 acaagccaaa caagagcgtc cagcagttaa gtccgatttt tcaagtccaa aagtggaacg
    11701 ccaaggcaat accgttcaag aaaaaaccgt tcaaaagcca gcaacttcaa ccactacagc
    11761 agatagaact tcacaacgtc caatcacaaa agaacgtccg tctactgttc aaagagtacc
    11821 actacaaaat acaagaagta gaccaccaat caaaaccgcc accattaaga aagtcggtaa
    11881 gaaaccatga agttgaaaac tttagtgatt ggtggttctg gattattctt gatggtcttc
    11941 tcactgcttc tgtttgttgc cattttattt tcagatgaac aggacagcgg aatttccaat
    12001 attcattatg gaggtgtgaa tgtttccgca gaagtgctgg ctcataagcc tatggtagaa
    12061 aaatatgcca aagaatatgg cgttgaagaa tatgtcaaca tacttcttgc gattatacag
    12121 gtggaatcgg gcggtactgc ggaagatgtt atgcagtcct cggaatccct cggtcttcca
    12181 cctaattcat tgagtacaga agaatccatt aagcaaggtg tgaagtattt cagtgaatta
    12241 ttagccagta gcgaaaggct cagtgtagat ttagaatcgg ttatccagtc ctacaattat
    12301 ggtggtggtt tcttagggta tgtggctaat cgtggaaata aatatacctt tgaactggct
    12361 caaagtttct caaaagagta ttcaggtggc gaaaaagtgt cttaccccaa tcccatagcc
    12421 atacctatca atgggggctg gcgatacaac tatggcaata tgttttatgt gcaactggta
    12481 acgcagtatc ttgtcacaac agagtttgat gatgatacgg tacaagccat catggacgaa
    12541 gcactgaaat atgagggctg gcgatacgtt tacggtggag cttccccgac tacttctttt
    12601 gattgtagcg gactgacaca atggacgtat ggaaaagctg gaattaactt accacgaacc
    12661 gcacaacagc aatatgatgt gacccagcat atcccactat cggaagcaca agctggcgat
    12721 ttggttttct ttcattctac ctataacgct ggctcttata ttactcatgt tgggatatac
    12781 cttggcaata accgtatgtt tcatgcaggc gacccaatcg gttatgccga cttaacaagc
    12841 ccctactggc aacagcattt agtgggagca ggacgaatca aacaatgaga aaggaagatt
    12901 taatgatgaa atttagaaaa aatcagaata aagaaaaaca gataccaaag gaaaagaaac
    12961 ctcgtgtcta ctataaggtc aatcctcata aaaaggttgt gattgccttg tgggtacttt
    13021 tagggcttag tttcagcttt gcgatattca agcactttac agctatagat actcatacta
    13081 ttcacgaaac aactatcata gaaaaggaat acgttgatac tcatcatgta gaaaattttg
    13141 tagagaactt tgcgaaagtc tactattcat gggagcaatc cgataagtcc attgataatc
    13201 gaatggaaag tctaaaaggc tatctgacag atgaacttca agctctcaat gttgatacag
    13261 tacgcaaaga tattcctgta tcgtcttctg taagaggatt tcagatatgg acggtagagc
    13321 caactggcga caatgagttt aatgtaacct acagtgtaga ccagctcatt acagagggag
    13381 aaaatacaaa gaccgtccac tctgcttata tagtgagtgt ctatgtagat ggttctggaa
    13441 atatggtact ggttaagaat ccgaccatta ccaacatacc taagaaatca agttataaac
    13501 caaaagccat tgaaagtgag gggacggttg attccattac aaccaatgaa atcaatgagt
    13561 ttttaacgac gttcttcaag ctctatccta cagcgacagc cagtgaactt tcctactatg
    13621 tgaatgacgg gatattaaaa ccaatcggaa aagagtacat ctttcaagaa ctggtaaatc
    13681 ctattcacaa tcgtaaggat aatcaagtca cggtatcgct gacagtggag tatatcgacc
    13741 agcagaccaa agcaacgcag gtatctcaat ttgatttggt acttgaaaag aacgggagta
    13801 attggaagat tatagaataa caaatattgg tacattatta cagctatttt gtaatcacgt
    13861 actctctttg ataaaaaatt ggagattcct ttacaaatat gctcttacgt gctattattt
    13921 aagtatctat ttaaaaggag ttaataaata tgcggcaagg tattcttaaa taaactgtca
    13981 atttgatagt gggaacaaat aattggatgt ccttttttag gagggcttag ttttttgtac
    14041 ccagtttaag aataccttta tcatgtgatt ctaaagtatc cggagaatat ctgtatgctt
    14101 tgtatgccta tggttatgca taaaaatccc agtgataaga gtatttatca ctgggatttt
    14161 tatgcccttt tgggcttttg aatggaggaa aatcacatga aaattattaa tattggagtt
    14221 ttagctcatg ttgatgcggg aaaaactacc ttaacagaaa gcttattata taacagtgga
    14281 gcgattacag aattaggaag cgtggacaga ggtacaacga aaacggataa tacgctttta
    14341 gaacgtcaga gaggaattac aattcagacg gcgataacct cttttcagtg gaaaaatact
    14401 aaggtgaaca tcatagacac gccaggacat atggattttt tagcagaagt atatcgttca
    14461 ttatcagtat tagatggggc aattctactg atttctgcaa aagatggcgt acaagcacaa
    14521 actcgtatat tgtttcatgc acttaggaaa ataggtattc ccacaatctt ttttatcaat
    14581 aagattgacc aaaatggaat tgatttatca acggtttatc aggatattaa agagaaactt
    14641 tctgcggaaa ttgtaatcaa acagaaggta gaactgcatc ctaatatgcg tgtaatgaac
    14701 tttaccgaat ctgaacaatg ggatatggta atagaaggaa atgattacct tttggagaaa
    14761 tatacgtctg ggaaattatt ggaagcatta gaactcgaac aagaggaaag cataagattt
    14821 cataattgtt ccctgttccc tgtttatcac ggaagtgcaa aaaacaatat agggattgat
    14881 aaccttatag aagtgattac gaataaattt tattcatcaa cacatcgagg tcagtctgaa
    14941 ctttgcggaa aagttttcaa aattgagtat tcggaaaaaa gacagcgtct tgcatatata
    15001 cgtctttata gtggcgtact gcatttgcga gattcggtta gaatatcgga aaaggaaaaa
    15061 ataaaaatta cagaaatgta tacttcaata aatggtgaat tatgtaaaat cgataaggct
    15121 tattccgggg aaattgttat tttgcagaat gagtttttga agttaaatag tattcttgga
    15181 gatacaaagc tattgccaca gagagagaga attgaaaatc ccctccctct gctgcaaacg
    15241 actgttgaac cgagcaaacc tcaacaaagg gaaatgttac ttgatgcact tttagaaatc
    15301 tccgacagtg acccgcttct gcgatattat gtggattctg cgacacatga aatcatactt
    15361 tctttcttag ggaaagtaca aatggaagtg acttgtgctc tgctgcaaga aaagtatcat
    15421 gtggagatag aaataaaaga gcctacagtc atttatatgg aaagaccgtt aaaaaaagca
    15481 gagtatacca ttcacatcga agttccaccg aatcctttct gggcttccat tggtctatct
    15541 gtagcacagc ttccattagg gagcggagta cagtatgaga gctcggtttc tcttggatac
    15601 ttaaatcaat cgtttcaaaa tgcagttatg gaggggatac gctatggctg tgaacaagga
    15661 ttgtatggtt ggaatgtgac ggactgtaaa atctgtttta agtatggctt atactatagc
    15721 cctgttagta ccccagcaga ttttcggatg cttgctccta ttgtattgga acaagtctta
    15781 aaaaaagctg gaacagaatt gttagagcca tatcttagtt ttaaaattta tgcgccacag
    15841 gaatatcttt cacgagcata caacgatgct cctaaatatt gtgcgaacat cgtagacact
    15901 caattgaaaa ataatgaggt cattcttagt ggagaaatcc ctgctcggtg tattcaagaa
    15961 tatcgtagtg atttaacttc ctttacaaat ggacgtagtg tttgtttaac agagttaaaa
    16021 gggtaccatg ttactaccgg tgaacctgtt tgccagcccc gtcgtccaaa tagtcggata
    16081 gataaagtac gatatatgtt caataaaata acttagtgta ttttatgttg ttatataaat
    16141 atggtttctt gttaaataag atgaaatatt ttttaataaa gatttgaatt aaagtgtaaa
    16201 ggaggagata gttattataa actacaagtg gatattgtgt cctgtatgtg gaaataaaac
    16261 acgattaaag ataagggaag atactgaatt aaaaaaattc cccctctatt gtccgaaatg
    16321 cagacaagaa aatttaattg aaataaagca gttcaaagta actgtgatta cagagccaga
    16381 cgcaaagacg cagagccgat aaaatgagat taatacaatc tcattttatc ggctctttcc
    16441 gttatgtatg gattctttta attagtcttc gatgtttctt gcttcgttga taccgctggc
    16501 taaagattcc attaaggata gttctttgtc tgtaaagcta tccatgtatt tctctatctg
    16561 taatcgtcgg gtgcttttta ccaagttatt agcaggtaag aaaaattcat caacggaaac
    16621 atgaagtaac gatacaaggt cataaagaac ttgtatgctg gggtgttgcc ctttattttc
    16681 aatattagtt aagtaccgtg ggtcaatttc aatcaatgct cccacttgtt cacgagttaa
    16741 acctcgtttc aatcgagctt ctttaatggc taaaccaaag gctctaaaat catatttatc
    16801 ttctttttta cgcatagtag accacctcta tacattttat tgttcctact gaattaaaaa
    16861 caggtataga aaaacgtgtt atatggttta taggtttata tttaataaaa agcactacta
    16921 aacgccaata aaaaaaaccg ttatatggta gtgctattta cgctgttaaa atattgtata
    16981 ttacttccaa atggcggttt gttggaggtc aacgtcgcca tgaagtacat catatacaat
    17041 aaatttcctt acattgggtt cttgtcaaaa aaagtcgtct atctgcaata gataagtacg
    17101 tccaccaatg tggttttata aatcatatag atagaataac agaagcatgt aaacagagaa
    17161 ataaatctgt ttatatgctt ttttggctat tcagaacttt tttacaaagt ttatttatca
    17221 gtaatgcaac aaatccccct ttcacattgg gactaagagt gaaaggagat aaacgagcaa
    17281 ggctcacttc ctttcctaga cagaaagggg gtgagaaaca tgaaaccatc ttcttttcag
    17341 accacaatag aaaatcagtt tgactatatc tgtaaacgtg ctatggaaga cgagcgaaag
    17401 aattatatgc tttatctttc aaggattgca aagcgtgagg tgtccttttc ggatgttggc
    17461 gattatcttg ttagccagtt tgcgacaaca gataactatt caactgactt tcagattttt
    17521 acactcaatg ggttatcagt aggcgttgaa aatgatttgt tgagtgaagc attacgtgag
    17581 ttgccagaca agaaacgtga aattctactg ctgttttact ttatggacat gagcgattca
    17641 gaaattgcag acctgttgaa attgaaccgt tctactgtct atcggcatag aaccagtgga
    17701 ctagccttaa ttaaaaagtt tatggaggaa tttgaagaat gaaaacacaa tatcctatga
    17761 ttccctttcc tctcattgta aaggcaacag atggcgatac cgaagcgatt aaccagattc
    17821 tacatcatta cagagggtac ataacgaagc gttccctacg acttatgaaa gatgaatatg
    17881 gcaatcaaag tatggtcgtt gatgaagtct tacgtggaag aatggaaacc agactgatta
    17941 caaagatttt gtcatttgaa attaagtaat atcctctctc ctttcgtgga agcgtgctaa
    18001 accattccac gcttcccgaa cagggaggtt tgttattcca ccaaagcata ttgagctttc
    18061 aatgtgtttt gataggctaa cgagccattg ttctttgaaa actgaataaa agtaatcgaa
    18121 tacgtttcga taagaaaaga gccaacggaa ctaaccgcca tgacctatct tataaagata
    18181 gcgagcgatt catgttagtg atccgagaag caatctttag caggattgcc tgcaacgaca
    18241 ttcttatcgt gataatgata ctcccataca gtcaatagtc cgagcgtgat aaaaccgtcg
    18301 caggcaatga gtatggctac atgagaacca tgcaggggtg gaactcccgt gagctttgct
    18361 aaagctgttc gattgctggt aaaacaactt ttatgaaatc caaataagtg atttggaaag
    18421 gaggatttta tgaagcagac tgacattcct atttgggaac gttataccct aaccattgaa
    18481 gaagcgtcaa aatattttcg tattggcgaa aacaagctac gacgcttggc agaggaaaat
    18541 aaaaatgcaa attggctgat tatgaatggc aatcgtattc agattaaacg aaaacaattt
    18601 gaaaaaatta tagatacatt ggacgcaatc tagcgtcgcc aaagggtctt gtatatgata
    18661 aaatagtatt aagtcgtatc aaggctcttt ccataaagga aaggagcaaa tgccatgtca
    18721 gaaaaaagac gtgacaataa aggtcgaatc ttaaagactg gagagagcca acgaaaagac
    18781 ggaagatact tatacaaata tatagattca tttggagaac cgcaatttgt ttactcgtgg
    18841 aaacttgtgg ctacagaccg agtaccagca ggaaagcgtg attgtatctc acttagagag
    18901 aaaatcgcag agttacagaa agacattcat gatggtattg atgttgtagg aaagaaaatg
    18961 acactctgcc agctttacgc aaaacagaac gctcaaagac caaaggttag aaaaaacact
    19021 gaaactggac gcaaatatct tatggatatt ttgaagaaag acaagttagg tgtaagaagt
    19081 attgacagta ttaagccatc agacgctaaa gaatgggcta ttagaatgag tgaaaatggt
    19141 tatgcttatc aaaccatcaa taactacaaa cgttctttaa aggcttcatt ctatattgct
    19201 atacaagatg attgtgttcg gaagaatcca tttgactttc aactgaaagc agttcttgat
    19261 gatgatactg tccctaagac cgtactaaca gaagaacagg aagaaaaact gttagccttt
    19321 gcaaaagctg ataaaaccta cagcaaaaat tatgatgaaa ttctgatact cttaaaaaca
    19381 ggtcttcgta tttcagagtt tggtggtttg acacttccag atttagattt tgagaatcgt
    19441 cttgtcaata tagaccatca gctattgaga gatactgaaa ttgggtacta cattgaaaca
    19501 ccaaagacca aaagtggcga acgtcaagtt cctatggttg aagaagccta tcaagcattt
    19561 aagcgagtgt tagcgaatcg aaagaatgat aagcgtgttg agattgatgg atatagtgat
    19621 ttcctctttc ttaatagaaa gaactatcca aaagtggcaa gtgattacaa cggcatgatg
    19681 aaaggtcttg ttaagaaata caataagtat aacgaggata aattgccaca catcactcca
    19741 catagtttgc gacatacatt ctgtaccaac tatgcaaatg caggaatgaa tccaaaggca
    19801 ttacagtaca ttatgggaca tgctaatata gccatgacgc tgaactatta cgcacatgca
    19861 acattcgatt ctgcaatggc agaaatgaaa cgcttgaata aagagaagca acaggagcgt
    19921 cttgttgctt agtagtacaa atgaatttac tacttattta ccacttctga cagctaagac
    19981 atgaggaaat atgcaaagaa acgtgaagta tcttcctaca gtaaaaatac tcgaaagcac
    20041 atagaataag gctttacgag catttaagaa aatataaaaa gataattaga aatttatact
    20101 ttgttttact taaaaataaa tgttcttttt catttattga tccactgcga ttaatttcat
    20161 catctttatt atgggttata cagtaagtga tttcatcatt atctgaaaaa ataattttca
    20221 cagtactacg ccaatcacct ggcattgttg ttgttattgg attggaaatt tgaatctcct
    20281 taatatctga atatgaattt ttcaatgctt tgactagact aacctcatat tctcgattcc
    20341 gttttagttc ttcttttctt tgttgttctt tcatataaaa tcctccgcta aaaataagt
//
LOCUS       DATRUK010000032        20091 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.032, whole genome shotgun sequence.
ACCESSION   DATRUK010000032 DATRUK010000000
VERSION     DATRUK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20091
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.032"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(47..832)
                     /locus_tag="VBE34_001166"
     CDS             complement(47..832)
                     /locus_tag="VBE34_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEO7263888.1"
                     /translation="MTYTSLKSPTTKDYKYTLNVAHLYGNLLNTYGDNGNILMMKYVG
                     EKLGCQMTFDIVSLEDRFDPNYYQMAFFGGGQDYEQAIVARDLPSKKEDINKFIQNNG
                     VVLAICGGFQLLGQYYIQANGERIEGIGVMGHYTLNQNNNRYIGDIKIHNDEFNETYY
                     GFENHQGRTFLSEDEKPLGTVIYGNGNNKEDGTEGVHYKNVFGSYFHGPILSRNANLA
                     YRLVATALRNKYGKEIVLPSYEEILSLEIPEEYGDVKSKADFE"
     gene            complement(832..2175)
                     /locus_tag="VBE34_001167"
     CDS             complement(832..2175)
                     /locus_tag="VBE34_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEO7263889.1"
                     /translation="MKINTALGVAAGKSAHYLLSKMGRGSTYPGSLALKFDKDILDTI
                     AKDYEIVVVTGTNGKTLTTALTVGILKEAFGQVVTNPSGANMITGIVSTFLTAKKSKS
                     GKKIAVLEIDEASLPRITQYIKPSLFVFTNIFRDQMDRYGEIYTTYQMILDGAANAPQ
                     ATILANGDSPLFNSKSVTNPVQFYGFDTDKHEPRLAHYNTEGILCPKCQAILTYRLNT
                     YANLGDYTCPNCDFERPNLDYALTRLIHLTNTSSGFVIDGQQYNINVGGLYNIYNALA
                     AVSVAEYFGVEASQIKDGFDKSRAVFGRQETFTIGNKKCTLVLIKNPVGASQALDMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDANFETILTMNIPEIFAGGVRHSEIARRLRVT
                     GYDEKRIKQADKLQDIMAMIEQQETEHAYILATYTAMLEFREILANHNAIRKEMN"
     gene            2315..3166
                     /locus_tag="VBE34_001168"
     CDS             2315..3166
                     /locus_tag="VBE34_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEO7263890.1"
                     /translation="MDIFSAIDSKFWASIMENPWMILIHLMDIIIVAVLIYKFIKALA
                     GTKIMSLIQGVILFIIIRFVSEWIGLTTITFLMNQIVTYGVIAGVVIFAPEIRTGLEK
                     FGRTPQLFTQRSQLSSDEKLVDALVKAVAYMSPRKIGALISIERTQTLQEYIATGIPL
                     DADISSELLINIFIPNTPLHDGAVIVKDKKIATACSYLPLSESSSISKEFGTRHRAAI
                     GLSENSDALTVIVSEETGGISVALKGEFLHDLSKDSFEAILRTQLIQNQEENSKLAWY
                     NQLLRRK"
     gene            3169..4128
                     /locus_tag="VBE34_001169"
     CDS             3169..4128
                     /locus_tag="VBE34_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEO7263891.1"
                     /translation="MKKFFTNKFWLGVVSLFLAILLFLTATATSMNHQDNSKIAGASE
                     TYTHTLTDVPIDIKYDSDDYFISGYSYGADVYMSSVNRVKLDSEINEDTRKFKVVADL
                     TNMKPGTHKVPLKVVNLPSGVNATVSPTTITVTMGKKKTKEFPVYGHVNDKQIKAGYA
                     VDKMSVDVSKVKVTSDESIIDRIDHVAANIPDDKVLDDDFNKTVTLQAVTADGTVLAS
                     IIHPSKATLSVKVKKLTKTVPINLIPVGQFSDSISKINYKLSQEKAVISGTKEALEAI
                     SVINAEVDISDVTKNTEKKINLSANNVSVDPAQVTVQLTTTKK"
     gene            4182..5534
                     /locus_tag="VBE34_001170"
     CDS             4182..5534
                     /locus_tag="VBE34_001170"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEO7263892.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETDRPRV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGSDGFKLDDDRELEIEALLDAKEDTLPRPSAQGLGTLVDYPEGLRKY
                     EKFMESTGIDLEGMNVALDTANGAATASARNIFLDLNADISVIGDQPDGLNINDGVGS
                     THPEQLQSLVRENGSDIGLAFDGDSDRLIAVDENGEIVDGDKIMFIIGKYLSDKGQLA
                     QNTIVTTVMSNLGFHKALDREGIHKAITAVGDRYVVEEMRKSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLTKVMKETGKKLSELASEVTIYPQKLVNIRVENNMKDKAMEVPAI
                     AEIIAKMEEEMDGNGRILVRPSGTEPLLRVMAEAPTNEAVDYYVDTIADVVRTEIGLD
                     "
     gene            5657..6028
                     /locus_tag="VBE34_001171"
     CDS             5657..6028
                     /locus_tag="VBE34_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEO7263893.1"
                     /translation="MTKEAELLWAKHKYLVLSKSQKIYLDIRQTLKSPNCTVLDVQSL
                     IDQAVLLEESPSQVTNAYMHIWGYFKNKAERQEKEEFLTLLEKYRKTGYQRRKLLAFL
                     KQLLAKYPNSYLQNSSIFEEE"
     gene            6053..6433
                     /locus_tag="VBE34_001172"
     CDS             6053..6433
                     /locus_tag="VBE34_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263894.1"
                     /translation="MRLWHQDIIELLPRQQLLGQHRECCALRGNGWGRKHETVDYVFR
                     YSPYRLFAYHQLVMEEMMERGYRVSKEWLIAEYRGMKCPRYDTLNPVDLETPIYPEHN
                     QDYLQECLWNLKAKGIDLPINKIK"
     gene            6526..7656
                     /locus_tag="VBE34_001173"
     CDS             6526..7656
                     /locus_tag="VBE34_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEO7263895.1"
                     /translation="MLKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDAYLQALIRE
                     FRSYDITELRTLYIGGGTPTSISAVQLDYLLTELSRDLNLNTLEEFTIEANPGDLTVD
                     KIEVLQKSAVNRVSLGVQTFNDKHLKRIGRSHNEVQIYSTIDALKTAGFQNISIDLIY
                     ALPGQTMDDVRSNVAKALSLNIPHLSLYSLILEHHTVFMNKMRRGKLHLPTEDLEAEM
                     FEYIISEMERNGFEHYEISNFTKPGFESRHNLMYWDNVEYYGVGAGASGYLDGIRYRN
                     RGPIQHYLKGVSEGNARLSEEVLSKNEMMEEELFLGLRKKKGVSIGKFEQKFGTSFEK
                     RYGQIVQELQSDGLLKENNGFIQMTKKGLFLGDTVAEKFIVE"
     gene            7660..8397
                     /locus_tag="VBE34_001174"
     CDS             7660..8397
                     /locus_tag="VBE34_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEO7263896.1"
                     /translation="MGLLYRETYEVPFYESDTNHYMKLPQLLALALQISAKQSLKLGI
                     GDDIVFKRYGLVWVVTDYIIDIERLPKHAEKIVIETEAKAHNKLLCYRYFYIYGEDGQ
                     KIITISSAFVLMDFKTRKIHPVLDDITSIYQSQRIKKVIRGPKYHPIGDSKVKQYHVR
                     YFDLDMNGHVNNSKYLEWMYDVLDLDFLSSHIPKKIDLKYIKEIQYGTDIKSHWYQDG
                     LVTRHDIIGGDAIHAQARIEWQEKKED"
     gene            8398..9168
                     /locus_tag="VBE34_001175"
     CDS             8398..9168
                     /locus_tag="VBE34_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEO7263897.1"
                     /translation="MAYKGYLIDLDGTIYKGKSRIPAGERFIERLQEKGIPYMLVTNN
                     TTRTPESVQEMLRGFNVETPLETIYTATMATVDYMNDMNRGKTAYVIGEEGLKKAIAD
                     AGYVEDTKNPAYVVVGLDWNVTYDKLATATLAIQNGALFIGTNPDLNIPTERGLLPGA
                     GSLNALLEAATRIKPVFIGKPNAIIMNKALEILNIPRNQAVMVGDNYLTDIMAGINND
                     IDTLLVTTGFTTVEEVPDLPIQPSYVLASLDEWTFNEG"
     gene            9158..9814
                     /locus_tag="VBE34_001176"
     CDS             9158..9814
                     /locus_tag="VBE34_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEO7263898.1"
                     /translation="MKDKLLVVLTWIWIISLATLATIYIAWLIYPIEIQFLKLEKVVY
                     LKAETIYYNFNKLMIYLTHPFISDLNMPSFPSSEDGLKHFADVKYLFTLAHGLFVILT
                     FPVIYFLRRGWKQKSIFLYEGFFKIAIMLPIFIVVCAFLLGFDQFFTLFHEVLFPGDS
                     TWQFNPLTDPVIWILPETFFLHCFIIFLLIYETITIILLIIGRKHLKLRKYKNKMQAL
                     "
     gene            10094..14206
                     /gene="cas9"
                     /locus_tag="VBE34_001177"
     CDS             10094..14206
                     /gene="cas9"
                     /locus_tag="VBE34_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040090.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEO7263899.1"
                     /translation="MNKPYSIGLDIGTNSVGYSVVTDDYKVPAKKMRVLGNTDKEYIK
                     KNLIGALLFDGGNTASDRRLKRTARRRYTRRRNRILYLQEIFAEEMSKVDDSFFHRLE
                     DSFLVEDDKRGSKYPIFATMQEEKDYHEKFPTIYHLRKELADKKEKADLRLVYLALAH
                     IIKFRGHFLIEDDSFDVRNTDIQRQYQAFLEIFDTTFENNHLLSQNIDVEGILTDKIS
                     KSAKKDRILAQYPNQKSTGIFAEFLKLIVGNQADFKKHFNLEDKTPLQFAKDSYDEDL
                     ENLLGQIGDEFADLFSVAKKLYDSVLLSGILTVTDLSTKAPLSASMIQRYDEHREDLK
                     QLKQFVKASLPEKYQEIFTDSSKDGYAGYIEGKTNQGAFYKYLSKLLTKQEGSEYFLE
                     KIKNEDFLRKQRTFDNGSIPHQVHLTELKAIIRRQSEYYPFLKENLDRIEKILTFRIP
                     YYVGPLAREKSDFAWMTRKTDDSIRPWNFEELVDKEASAEAFIHRMTNNDLYLPEEKV
                     LPKHSLIYEKFTVYNELTKVRYKNEQGETYFFDSNIKQEIFDGVFKEHRKVSKKKLLD
                     FLAKEYEEFRIVDVIGLDKENKAFNASLGTYHDLKKILDKDFLDNPDNESILEDIVQT
                     LTLFEDREMIKKRLENYKDLFTESQLKKLYRRHYTGWGRLSAKLINGIRDKESQKTIL
                     DYLISDGRANRNFMQLIHDDGLSFKPIIDKAQAGSHSDNLKEVVGELAGSPAIKKGIL
                     QSLKIVDELVKVMGYEPEQVVVEMARENQTTNQGRRNTRQRYKLLEEGVKNLASDLNG
                     NILKEYPTDNQALQNERLFLYYLQNGRDMYTGEALDIDNLSQYDIDHIVPQAFIKDDS
                     IDNRVLVSSAKNRGKSDNVPSIDIVKARKAFWKKLLDAKLMSQRKYDNLTKAERGGLT
                     SDDKARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIRKVKIVTLKSNLVSNFR
                     KEFGFYKIREVNDYHHAHDAYLNAVVAKAILTKYPQLEPEFVYGDYPKYNSYKTRKSA
                     TEKLFFYSNIMNFFKTKVTLADGTVVVKDDIEVNNDTGEIVWDKKKHFATVRKVLSYP
                     QVNIVKKTEIQTGGFSKESILAHGNSDKLIPRKTKDIYLDPKKYGGFDSPIVAYSVLV
                     VADIKKGKAQKLKTVTELLGITIMERFRFEKNPSAFLESKGYLNIRDDKLMILPKYSL
                     FELENGRRRLLASAGELQKGNELALPTQYMKFLYLASRYNELKGKPEEIEQKQEFVVQ
                     HVSYFDDILQIINDFSNRVILADANLEKINKLYQDNKENISVDELANNIINLFTFTSL
                     GAPAAFKFFDKIVDRKRYTSTKEVLNSTLIHQSITGLYETRIDLGKLGED"
     gene            14208..15077
                     /gene="cas1"
                     /locus_tag="VBE34_001178"
     CDS             14208..15077
                     /gene="cas1"
                     /locus_tag="VBE34_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEO7263900.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLVFKDSYQTEMIHLSEIDILIME
                     TTDIVLSTMLIKRLVDENILVIFCDDKRLPTAMLMPYYARYDSSLQLSRQMSWIEDVK
                     ADVWTSIIAQKILNQSFYLGECSFFEKSQSIMNLYHDLESFDTSNREGHAARIYFNTL
                     FGNDFSREHDNPINAGLDYGYSLLLSMFAREVVKCGCMTQFGLKHANQFNQFNLASDI
                     MEPFRPIVDRIIYENRQSDFVKMKRELFSMFSETYSYDGKEMYLSNIVSDYTKKVIKS
                     LNSDGNGIPEFRI"
     gene            15074..15415
                     /gene="cas2"
                     /locus_tag="VBE34_001179"
     CDS             15074..15415
                     /gene="cas2"
                     /locus_tag="VBE34_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEO7263901.1"
                     /translation="MSYRYMRMILMFDMPTETVEERKAYRKFRKFLLSEGFIMHQFSV
                     YSKLLLNNTANNAMIGRLKVNNPKKGNITLLTVTEKQFARMVYLHGERNTSVANFDSR
                     LVFLGDSYDQD"
     gene            15402..16067
                     /gene="csn2"
                     /locus_tag="VBE34_001180"
     CDS             15402..16067
                     /gene="csn2"
                     /locus_tag="VBE34_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEO7263902.1"
                     /translation="MIKINFPILDEPLVLSNATILTIEDVSVYSSLVKHFYQYDVDEH
                     LKLFDDKQKSLKATELMMVTDILGYDVNSAPILKLIHGDLENQFNEKPEVKSMVEKLA
                     ATITELIAFECLENELDLEYDEITILELIKALGVKIETQSDTIFEKCFEIIQVYHYLT
                     KKNLLVFVNSGAYLTKDEVIKLCEYINLMQKSVLFLEPRRLYDLPQYVIDKDYFLIGE
                     NMV"
     repeat_region   16168..17061
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=16168..16203
                     /rpt_unit_seq="gttttagagctgtgctgtttcgaatggttccaaaac"
     gene            17340..17477
                     /locus_tag="VBE34_001181"
     CDS             17340..17477
                     /locus_tag="VBE34_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263903.1"
                     /translation="MVKGLDCSTLYLIISLTALVLLVIAGVSLSLILASLQGKQWRTY
                     P"
     gene            17618..18034
                     /gene="ndk"
                     /locus_tag="VBE34_001182"
     CDS             17618..18034
                     /gene="ndk"
                     /locus_tag="VBE34_001182"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEO7263904.1"
                     /translation="MEQTFFMIKPDGVKRGFIGEVISRIERRGFSIDRLEVRHADADI
                     LKRHYAELTDRPFFPTLVDYMTSGPVIIGVISGEEVISTWRTMMGSTNPKDALPGTIR
                     GDFAQAPSPNQATCNIVHGSDSPESAAREIAIWFNN"
     gene            18170..20002
                     /gene="lepA"
                     /locus_tag="VBE34_001183"
     CDS             18170..20002
                     /gene="lepA"
                     /locus_tag="VBE34_001183"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEO7263905.1"
                     /translation="MNIEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRAEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGEVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGMVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYIAASIKTVAD
                     TRVGDTITLANNPAIDPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVNTTDGEMLE
                     VSNPSEFPDPTRVDSIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDDKK"
BASE COUNT         6634 a         3070 c         3975 g         6412 t
ORIGIN      
        1 cacgtacggt ggtgtgagag gggctagaga ttagcctcta ctcgatttat tcaaaatctg
       61 cttttgattt aacgtctccg tattcctcag gaatctctaa agataaaatt tcttcataac
      121 tagggaggac aatctcttta ccatatttgt tacggagggc agttgccaca agacggtaag
      181 ctagattagc attacgtgaa aggattggac cgtgaaagta tgacccaaaa acattcttat
      241 aatgaacccc ttcagtacca tcttctttgt tattcccatt tccataaatg acagttccta
      301 aaggtttttc gtcttctgat aaaaaggtac gtccttgatg attctcaaag ccataatatg
      361 tttcattgaa ttcatcgttg tgaatcttga tatcaccaat atagcgatta ttattttggt
      421 ttagcgtata gtgtcccata acgccaatgc cctctattcg ttctccatta gcttgaatat
      481 agtattgtcc taaaagttgg aaacctccac aaatagcaag tactacacca ttattttgaa
      541 tgaatttgtt aatgtcttct ttcttactgg gaagatcgcg tgcaacaata gcttgttcgt
      601 agtcttgtcc accaccaaaa aatgccatct gataatagtt gggatcaaat ctatcttcta
      661 atgaaacgat atcaaatgtc atttgacatc ctagtttttc tcccacatat ttcatcatta
      721 agatattccc attatcgcca taagtattta ataggttacc ataaagatga gctacattaa
      781 gagtgtattt ataatctttg gtagtaggag attttagtga ggtataagtc attaattcat
      841 ctcctttctg atagcgttgt ggttagcaag aatttcacga aactccagca tagctgtata
      901 tgttgctaaa atataagcat gctcagtctc ttgctgctca atcattgcca taatatcttg
      961 cagtttatca gcttgtttta tgcgtttctc atcataacca gttacacgta agcgacgagc
     1021 aatttcagag tggcgaacac caccagcaaa tatttctggg atattcatcg tgagaatagt
     1081 ttcaaaatta gcgtcccaaa tccaagaagt atcaataccg tcagcataat tggcatttaa
     1141 tagtactgaa agagagaacg ggtatggagc aagtttaatc atatcaagag cttgactagc
     1201 tcctactgga tttttaatca gaactaaggt acatttttta tttccaattg taaaggtttc
     1261 ttgacgacca aaaacggcac ggcttttatc aaatccatct ttaatttgac ttgcttctac
     1321 accaaaatat tcagcaacag atactgccgc caaagcattg taaatattat aaaggccacc
     1381 cacgttgata ttatattgtt ggccatcaat aacgaaacct gatgaagtat ttgtcagatg
     1441 tattagcctt gttaaggcat aatcaaggtt tggacgttca aaatcacaat taggacaagt
     1501 gtagtcacca aggttggcat atgtgttcaa tcgataagtt aaaattgctt gacactttgg
     1561 acagagaata ccctcagtat tatagtgtgc taatcgtggc tcatgtttgt cggtatcaaa
     1621 tccatagaat tgcacagggt tagtcacgct tttagaatta aaaagtggac tgtcaccgtt
     1681 agctaagata gtagcttgcg gagcgttagc agctccatct aaaatcattt gataagttgt
     1741 gtagatttcg ccataacggt ccatttgatc acggaaaata ttggtaaaga cgaacaaact
     1801 aggctttata tactgtgtaa tacggggtaa actcgcttcg tctatttcaa ggacagctat
     1861 ttttttacca gatttacttt tcttagctgt taggaaggtt gaaacaatac ctgtaatcat
     1921 atttgctcca cttgggttgg ttaccacctg accgaaggct tcctttaaaa taccaactgt
     1981 taaagctgtt gttaaggttt taccgttagt accagtcaca actacaattt cataatcttt
     2041 ggcaattgta tctagtatat ctttatcgaa tttgagtgct aaactacccg gataagtaga
     2101 accacgtccc attttactta aaaggtaatg agcagactta ccggcagcca ctcctagtgc
     2161 tgtatttatt ttcataaaga tattttatca taaaaagatg ctttcttgct aaagaaaata
     2221 ttatgaaaac atggtataat gtagacgatg gaaaataaga aatcaaggag ttaagcaaga
     2281 aactcttcga caggctacca gtagagagga agatatggat attttttcag ctattgattc
     2341 aaagttttgg gcgagcatta tggaaaatcc atggatgatc cttattcatc tcatggatat
     2401 tatcatagta gctgttctga tttataaatt tatcaaagcg ctagcaggca caaaaattat
     2461 gtccttaatt cagggcgtta ttttgtttat catcattaga tttgtgtcag aatggatagg
     2521 gctaacaacg attacatttt tgatgaatca gattgttact tatggtgtaa ttgcaggtgt
     2581 tgtcatcttt gctccagaga ttagaacagg tctcgaaaaa tttgggcgaa caccacaact
     2641 atttacgcaa cgttctcaat taagcagtga tgaaaagcta gtagatgccc ttgttaaggc
     2701 agttgcttat atgagtccgc gtaaaatagg agccttgatt tcaattgaac gtacccaaac
     2761 tttgcaagag tatatagcta caggtatccc attagatgca gatatttcca gtgaattgtt
     2821 aattaatatt ttcattccta atacaccact gcacgatggt gctgttattg taaaagataa
     2881 aaaaattgct acagcatgtt cgtacttacc tctatcagaa tcatcttcga tttcaaaaga
     2941 atttggaaca cgccacagag cagcgattgg tctatcagag aattcagatg cactaacggt
     3001 tattgtttct gaagagactg gtggtatttc tgttgctttg aaaggtgaat ttttacatga
     3061 tctttctaaa gatagctttg aagcaattct gagaactcag ttgattcaaa atcaagagga
     3121 aaactcaaaa ttagcttggt ataatcaact attgaggagg aagtagacat gaaaaaattt
     3181 tttacaaata aattttggtt aggcgttgtc tcactctttc tggctatttt attattttta
     3241 acggcaactg caacaagtat gaaccatcaa gacaatagta agattgcagg agcgtctgag
     3301 acatatacac ataccctaac agatgtgcca atagacatca aatacgatag tgatgattac
     3361 tttattagtg gctattctta tggggcagat gtctatatgt catcggttaa tcgtgttaag
     3421 ctagattctg aaataaatga ggatacacga aaatttaaag tagtcgctga tttgacaaat
     3481 atgaaaccag ggactcacaa agtcccacta aaggtagtaa atttgccatc tggtgtaaat
     3541 gctacggttt ctccaacaac aattacagta actatgggta aaaagaaaac aaaagagttt
     3601 ccggtttatg gacatgttaa tgataagcaa attaaagctg gttatgcagt cgataagatg
     3661 tcagttgatg tgtcaaaagt taaagttact agtgatgaat ctattataga tcgtattgac
     3721 catgtcgctg ccaatatccc tgatgataaa gttcttgatg atgattttaa caagacagtt
     3781 actttacaag ctgtaacagc agatggtaca gtattagcaa gtattattca cccttctaaa
     3841 gcaactttat ctgtcaaggt aaaaaaattg accaagacag taccgattaa tttgatacca
     3901 gtaggacaat tcagcgatag tatttcaaaa attaactaca agttgagtca agaaaaagca
     3961 gtaatttcag gaacaaaaga ggctttagaa gctatttctg ttattaatgc tgaggtagat
     4021 atttcagacg taactaaaaa tacagaaaag aaaattaatc tttctgcaaa caatgtttca
     4081 gttgatcctg ctcaagtaac agttcaattg acaactacta aaaaataata gataataaaa
     4141 acgaaaaaaa tggaggtagc gtttttcgct agaaaaaaat tatgggaaaa tattttggta
     4201 cagatggtgt tcgcggtgaa gctaatgttg aactgacgcc agaattggct tttaaattag
     4261 gacgttttgg aggatatgtt ctaagtcaac acgaaactga tcgtccgaga gtttttgtag
     4321 ctcgtgatac tcgtatttct ggtgaaatgc tggaatctgc tttgattgct ggtctacttt
     4381 cagtaggtat tgaggtttat aaacttggtg ttttagccac tcctggagtg tcttaccttg
     4441 ttcgtacaga aaaagctagt gcaggggtta tgatttcagc aagccacaat ccagcacttg
     4501 ataatggtat taagttcttt ggtagtgatg gttttaaact tgatgatgat cgtgaattag
     4561 aaattgaagc tttacttgat gcgaaggaag acactttacc acgtccatca gcacaaggtt
     4621 taggtacttt agtagattat cctgaaggac ttcggaaata tgagaagttt atggaatcaa
     4681 ctggtattga tttagaaggt atgaatgttg cgttggatac agcaaatggt gcagcaacag
     4741 cctcagctcg gaatatcttt ttagacttga atgctgatat aagtgttatt ggtgatcaac
     4801 cagatggttt gaatatcaat gatggtgttg gttcgacaca tcctgagcaa ttacaaagtt
     4861 tagttagaga aaatggttcc gacatcggtc tagcttttga tggtgatagt gatcgtttga
     4921 tcgctgttga tgaaaatgga gagattgtag atggtgataa aattatgttt atcattggga
     4981 aatatctctc tgataaaggg cagcttgctc agaatacaat cgtaacaacg gttatgtcaa
     5041 atctcggctt tcataaagcg ctcgaccgtg aaggaattca taaagcgatt accgctgtag
     5101 gagaccgcta cgtggttgaa gaaatgcgta aatcaggata taatcttggc ggagagcaat
     5161 caggccacgt tattatcatg gattacaata ctacagggga tggacaatta acagccatcc
     5221 aattgactaa ggtcatgaaa gaaactggta agaaattatc agagttagct agtgaagtga
     5281 cgatttatcc tcaaaaatta gtaaatattc gtgttgaaaa taacatgaaa gataaagcga
     5341 tggaagttcc tgcaattgcg gaaattattg ctaaaatgga agaagagatg gatggcaatg
     5401 gtcgtattct agtacgtcct agtggtactg agcctcttct tagagttatg gcagaagcgc
     5461 caacaaatga agcggttgat tattatgttg atactatcgc agatgtcgtt cgtacagaaa
     5521 ttggtttaga ttaaaacggc gttattctgc tataaagtcg tcaatgttat aaaataataa
     5581 agagagggaa ttggagtgga gcatcaagca tacgcctttc tctctctttt tgatttgaga
     5641 agggagttta gagaaaatga ctaaagaagc agaattatta tgggctaaac acaagtattt
     5701 agtcttaagt aaatctcaga aaatttattt ggatattaga caaactttga aatcaccaaa
     5761 ttgtaccgtt ttagacgttc agtcactcat tgaccaagca gtgcttttag aagaatcacc
     5821 atcacaagtg actaatgctt atatgcatat atggggctat tttaaaaata aggcagagcg
     5881 tcaagaaaaa gaagaatttt taaccttgct ggaaaaatac aggaaaacag gttatcaacg
     5941 aagaaaactg ttagcttttc taaaacagct attagctaaa tacccaaatt cttatttaca
     6001 aaactcttct attttcgaag aagagtaaaa gattgatagt tgaggtatat aaatgagatt
     6061 atggcatcaa gatattattg agttattgcc taggcagcaa cttttaggac agcatagaga
     6121 gtgttgtgca ttgagaggga atgggtgggg gagaaaacat gagactgttg attatgtttt
     6181 tcgatattca ccttataggt tatttgcata tcatcaatta gttatggaag aaatgatgga
     6241 gcgtgggtat cgtgtatcta aagaatggct aattgcagaa tataggggaa tgaagtgccc
     6301 aagatatgat acacttaatc ctgttgactt ggagacacct atctatcctg agcacaatca
     6361 agactatcta caagaatgtc tatggaattt aaaagcaaaa ggaattgacc ttcctataaa
     6421 taagataaaa tagagattta tctctatttt tattttggaa taaaaagcta tattatcaag
     6481 ttttagagga gtgttttttt aagggcaaag tggtaaaata gaaacatgtt aaagaaacca
     6541 acctcagcat atgtacacat tccattttgt acccaaatat gttattattg tgatttttca
     6601 aaagttttta ttaaaaatca gcctgtagac gcttatcttc aggctcttat ccgtgaattt
     6661 cggtcttatg atattacaga attgcgcacc ttatatattg gaggagggac acctacatct
     6721 ataagcgctg tgcaactgga ctatttattg acggagttaa gtagagattt aaacttaaat
     6781 actttagagg agttcactat cgaagctaat ccaggagatt taaccgttga taaaatagag
     6841 gtgcttcaaa agtcagctgt caatagagtt tctctaggag ttcagacctt taatgataaa
     6901 catttaaagc gtattggacg cagtcacaat gaggtacaaa tttattctac aattgatgct
     6961 cttaaaacag ctggttttca aaatatctct attgacctaa tttatgcctt acctggacaa
     7021 acgatggatg atgttagaag taatgtagcc aaagctctct ctctaaatat tcctcatttg
     7081 agtctatata gtttaatttt agaacaccat actgtattta tgaataaaat gcgtcgtggg
     7141 aaattacatt tgccaacaga agatttagaa gccgagatgt ttgaatatat catttctgaa
     7201 atggagagaa atggttttga gcattatgaa atttctaatt ttacaaagcc gggttttgaa
     7261 agtcgtcata atttgatgta ctgggataat gtagaatatt atggtgttgg tgcaggtgct
     7321 tctggttatc ttgatggtat tcgttatcgt aatagagggc caatacaaca ttatctaaag
     7381 ggtgtttcag aaggtaatgc aaggttatct gaagaagtat tatcaaaaaa tgaaatgatg
     7441 gaagaggagc ttttcctcgg tcttcgtaaa aaaaagggtg tttccatcgg aaaatttgaa
     7501 caaaaatttg gaacgtcctt tgagaaacgt tacggtcaga ttgttcaaga actccaaagt
     7561 gatgggcttt tgaaagaaaa taatggtttt atccagatga ctaaaaaagg tctcttctta
     7621 ggagatacgg ttgccgagaa atttattgtg gagtaaaaaa tgggtttatt atatcgagaa
     7681 acttacgaag tgccttttta tgaatcagac actaatcatt atatgaaatt accgcagttg
     7741 ttggctttag cgttacaaat ttcagccaaa caatctttaa aattaggaat aggcgatgac
     7801 atcgttttta aaagatacgg attggtttgg gtcgttacag attatattat tgatattgag
     7861 cgtttaccca agcatgctga aaagatagtc attgagactg aagccaaagc tcataataaa
     7921 ttattgtgtt accgctattt ttatatttat ggtgaagacg ggcaaaaaat aataacgatt
     7981 tcatctgctt ttgtcttaat ggattttaaa acacggaaga ttcatcctgt tcttgatgat
     8041 attacttcaa tttaccaatc ccaaagaatc aaaaaagtta ttcgagggcc gaaatatcat
     8101 cccattggag atagtaaagt taaacaatac catgttcgtt attttgattt agatatgaat
     8161 ggacatgtta ataatagtaa gtatctagaa tggatgtatg atgtgttgga cttagatttt
     8221 ctaagtagcc acattcctaa aaaaattgat ttaaaatata ttaaggaaat tcaatatgga
     8281 acggatatta agtctcattg gtatcaagat ggtcttgtga cacgtcatga tattattggt
     8341 ggcgatgcta ttcatgctca agctaggatt gaatggcagg aaaaaaagga ggattaaatg
     8401 gcttataaag gatatcttat tgatttagat ggtactattt acaaagggaa aagtcgtatc
     8461 ccagcaggag agcgttttat cgaacgtttg caagaaaaag gcattcctta tatgttagta
     8521 actaataata cgactcgcac accagaaagc gttcaggaaa tgcttcgagg cttcaatgtt
     8581 gaaacaccac ttgaaacaat ctataccgca actatggcta cggttgacta tatgaatgat
     8641 atgaaccgtg gaaaaacagc ttacgttatt ggtgaagaag ggcttaaaaa agctattgca
     8701 gatgcaggtt atgtggagga tacaaaaaat ccagcctatg tggttgttgg tttagattgg
     8761 aatgttactt atgataaatt ggcaactgct acgttagcaa ttcaaaatgg tgctttattt
     8821 attggaacca atccagattt gaatattcct actgaacgtg gtttattacc cggtgctggc
     8881 tccttaaatg ctttattaga agccgcaacg cgtataaaac cagtctttat tggaaagcca
     8941 aatgccatca ttatgaataa ggcacttgaa attttaaata ttcctagaaa tcaagcagtc
     9001 atggtcggtg ataattattt gacagatatc atggctggta ttaataatga tattgatact
     9061 ttgttagtaa caacgggttt tacaacagtt gaagaagtac cagatttacc aattcaacca
     9121 agttatgttt tagcaagttt agatgaatgg acatttaatg aaggataagt tattagtagt
     9181 tttaacttgg atttggatta tatcgttagc aactctagct acaatttata ttgcttggtt
     9241 gatctatcct atagaaattc agtttttaaa attagaaaag gttgtgtatt taaaggctga
     9301 aacaatttat tataatttta ataagttgat gatatatctg actcatcctt ttatctctga
     9361 tttaaatatg cctagctttc cttcttcaga ggatggttta aagcattttg cggatgtaaa
     9421 atatcttttt actctagcgc atgggctttt tgttatacta acttttccag ttatttattt
     9481 tttaagacgt ggatggaagc aaaaaagtat ctttctatat gaaggcttct tcaagatagc
     9541 tattatgttg cctattttta ttgtagtatg tgcttttctg ttgggttttg atcagttttt
     9601 cactctattt catgaggtgc tttttccagg agattctact tggcaattta atcctctaac
     9661 ggatccagta atttggatac ttcctgaaac ttttttcctc cattgtttca tcattttttt
     9721 gttaatctat gaaacaataa ctataattct tttgataata ggtagaaaac atttaaaatt
     9781 aagaaaatat aaaaacaaaa tgcaagcctt ataatagcgc ttgcattttg tttgctattt
     9841 ttttataata caaatatgag aaatgtcgga attataacaa tgacataaaa aaagcaccga
     9901 atcggtgctc actttttcaa gctaaatacg gactggatta aaaaatcact gccttatttt
     9961 aacgtgctgt gctgtttcga ataattccaa caaaattatt atatgttatt ttaatgtaat
    10021 aatcaagtaa aaattagcaa aactttaaag tttgcgtttt taaagtgata ttttaaataa
    10081 aaggagataa attatgaaca aaccatattc aataggtctc gacatcggaa ctaattccgt
    10141 cggatattct gtggttacag atgattataa ggtacctgct aagaagatga gggtcttagg
    10201 gaatacagat aaagaataca taaagaagaa tcttataggt gctctgcttt ttgatggtgg
    10261 aaatactgct tcagatagac gcttgaagcg aactgctcgc cgtcgttata cacgtcgtag
    10321 aaatcgtatt ctatatttac aagaaatttt tgcagaggaa atgagtaaag ttgatgatag
    10381 tttctttcat cgattagagg attcttttct agttgaggac gacaagagag ggagcaagta
    10441 tcctatcttt gcaacaatgc aggaggagaa agattatcat gaaaagttcc caacgattta
    10501 tcacttgaga aaagaattag ctgacaagaa agaaaaagca gaccttcgtc ttgtttatct
    10561 ggctctagct catatcatta aattcagagg acatttccta attgaggatg atagctttga
    10621 tgtgaggaat actgatattc aaagacaata tcaagctttt ttagaaatct ttgatactac
    10681 cttcgaaaat aaccatttgt tatctcaaaa catagatgta gagggaattc taacagataa
    10741 gattagcaag tctgcgaaga aagatcgcat tttagcgcag tatcctaacc aaaaatctac
    10801 tggcattttt gcagaatttt tgaaattgat tgtcggaaat caagctgact tcaagaaaca
    10861 tttcaatttg gaagataaaa cgccgcttca atttgctaag gatagctacg atgaagattt
    10921 agaaaatctt cttgggcaga ttggtgatga atttgctgac ttattctcag tagcgaaaaa
    10981 gctatatgat agtgttcttt tatctggcat tcttacagta actgatctca gtaccaaggc
    11041 gccactttct gcctctatga ttcagcgtta cgatgaacat agagaggact tgaaacagtt
    11101 aaaacaattc gtaaaagctt cattgcctga gaaatatcaa gaaatattta ctgattcatc
    11161 aaaagatggc tacgctggtt atattgaagg caaaactaat caaggagcat tttataaata
    11221 cttgtcaaaa ttgttgacca agcaagaagg tagcgagtat tttcttgaaa aaatcaagaa
    11281 tgaagatttt ttgagaaaac aaaggacctt tgataatggc tcaattccac accaagtcca
    11341 tttgacagaa ttgaaggcta ttattcgacg tcaatcagaa tactatccat tcttgaaaga
    11401 gaatctagat aggattgaaa aaatccttac ctttagaatt ccttattatg tcgggccact
    11461 agcacgtgag aagagtgatt ttgcatggat gactcgcaaa acagatgaca gtattcgacc
    11521 ttggaatttt gaagaattgg ttgataagga agcatctgcg gaagctttta tccatcgtat
    11581 gaccaacaat gacctctatc ttcctgaaga aaaagtccta ccaaaacata gtcttattta
    11641 tgaaaaattt acggtctata atgagttgac taaggttaga tataaaaatg agcaaggtga
    11701 gacttatttt tttgatagca atattaaaca agaaatcttt gatggagtat tcaaggaaca
    11761 tcgtaaggta tccaagaaga agttgctaga ttttctggct aaagaatatg aggagtttag
    11821 gatagtagat gttattggtc tagataaaga aaataaagct ttcaacgcct cattgggaac
    11881 ttaccacgat ctcaaaaaaa tactagacaa agattttctt gataatccag ataatgagtc
    11941 tattttggaa gatatcgtcc aaactctaac cttatttgaa gatagagaaa tgattaagaa
    12001 gcgtcttgaa aactataaag atctttttac agagtcacaa ctaaaaaaac tctatcgccg
    12061 tcactatact ggctggggac gattgtctgc taagttaatt aatggcatta gagataaaga
    12121 gagtcaaaaa acaatcttgg actatctgat ttctgatgga agagcaaatc gcaactttat
    12181 gcagttgata catgatgatg gtctatcatt taaaccaatt attgacaagg cacaggctgg
    12241 tagtcattca gataatctaa aagaagttgt aggtgagctt gcaggtagcc ctgctattaa
    12301 aaaggggatt ctacaaagtt tgaaaattgt tgatgagctt gttaaagtca tgggatacga
    12361 acctgaacaa gttgtggttg agatggcgcg tgagaatcaa acaacaaatc aaggtcgtcg
    12421 taacactcga caacgctata aacttcttga ggaaggcgtt aagaatctag ctagtgactt
    12481 gaatggcaat attttgaaag aatatcctac ggataatcaa gcgttgcaaa atgaaagact
    12541 tttcctttat tacttacaaa atggaagaga tatgtacaca ggggaagcac tagatattga
    12601 caatttaagt caatatgata ttgatcacat tgttcctcaa gctttcataa aagatgattc
    12661 tattgataat cgtgttttgg tatcatctgc taaaaaccgt ggaaaatcag ataatgttcc
    12721 tagcattgat attgtaaaag cacgcaaagc attctggaaa aaattacttg atgctaagtt
    12781 aatgagtcag cgtaagtatg ataatttgac taaggcagag cgcggaggcc taacttccga
    12841 tgataaggca agatttatcc aacgtcagtt ggttgagaca cgacaaatta ccaagcatgt
    12901 tgcccgtatc ttggatgaac gctttaataa tgagcttgat agtaaaggta gaaggattcg
    12961 caaagttaaa attgtaacct tgaagtcaaa tttggtttca aatttccgaa aagaatttgg
    13021 attctataaa attcgtgaag ttaatgatta tcaccatgca catgatgcct atcttaatgc
    13081 agtagttgct aaagctattc taaccaaata tcctcagtta gagccagaat ttgtctacgg
    13141 tgactatcca aaatataaca gttacaaaac gcgtaaatcc gctacagaaa agctattttt
    13201 ctattcaaat attatgaact tctttaaaac taaggtaact ttggcggatg gaaccgttgt
    13261 tgtaaaagat gatattgaag ttaataatga tacgggtgaa attgtttggg ataagaagaa
    13321 acactttgcg acagttagaa aagtcttgtc ataccctcag gtcaatatcg tgaagaagac
    13381 agagattcag acaggtggtt tctctaagga atcaatcttg gcgcatggta actcagataa
    13441 gttgattcca agaaaaacga aggatattta tttagatcct aagaaatatg gaggttttga
    13501 tagtccgata gtagcttact ctgttttagt tgtagctgat atcaaaaaag gtaaagcaca
    13561 aaaactaaaa acagttacgg aacttttagg aattaccatc atggagagat tcagatttga
    13621 gaaaaatcca tcagctttcc ttgaatcaaa aggttattta aatattaggg acgataaatt
    13681 aatgatttta ccgaagtata gtctgttcga attagaaaat gggcgtcgtc gattacttgc
    13741 tagtgctggt gaattacaaa aaggtaacga gctagcctta ccaacacaat atatgaagtt
    13801 cttatacctt gcaagtcgtt acaatgagct aaaaggaaaa ccagaagaaa ttgaacaaaa
    13861 acaggaattt gtagttcaac atgtttctta tttcgacgat attcttcaaa taatcaacga
    13921 tttttcaaat cgagttattc tagcagatgc taatttagag aaaatcaata agctttacca
    13981 agataataag gaaaatatat cagtagatga acttgctaat aatattatca atctatttac
    14041 ttttaccagt ctaggagctc cagcagcttt taaatttttt gataaaatag ttgatagaaa
    14101 acgctataca tcaactaaag aagtacttaa ttctacccta attcatcaat ctattactgg
    14161 actttatgaa acacgtattg atttgggtaa gttaggagaa gattgatatg gcaggttggc
    14221 gaaccgttgt tgtaaataca cattctaagc tctcttataa aaataatcat ctggttttta
    14281 aagattctta tcagacggaa atgattcatc tatcagagat tgacattcta atcatggaaa
    14341 caacagatat cgttttgtcg accatgctga ttaaacgttt ggttgatgaa aatattttag
    14401 ttatattttg tgatgacaaa cgcttgccaa cagctatgtt aatgccgtac tatgccagat
    14461 atgattcgag tttacaatta tctaggcaga tgtcatggat tgaagatgtc aaagcggatg
    14521 tttggacgtc aatcattgca caaaaaattt tgaatcagtc tttttatctt ggtgagtgtt
    14581 ctttctttga aaaatcccag tctattatga atctctatca tgatttagaa tcttttgata
    14641 cttccaatcg tgaagggcat gcagctagga tttatttcaa tacacttttt ggaaatgatt
    14701 tttcaagaga acatgataat ccaataaatg ctggtttaga ctacggatat tcattgcttt
    14761 tgagtatgtt tgcgcgtgaa gtcgttaagt gtggttgcat gacacaattt gggttaaagc
    14821 atgctaatca atttaatcag ttcaacctag caagcgatat tatggaacca tttcgcccaa
    14881 tcgttgatag gattatttat gaaaataggc agagtgattt tgtcaaaatg aaaagagaac
    14941 tcttttctat gttttcagag acatacagtt acgacggtaa agaaatgtat ctttcaaata
    15001 ttgtcagcga ttacaccaaa aaagttatta agtcgctaaa tagtgatggg aatgggattc
    15061 cggagtttag gatatgagtt atcggtatat gagaatgatt ttaatgtttg atatgcctac
    15121 tgaaacagta gaagaacgta aggcgtatcg taagtttaga aagtttcttt tgagcgaagg
    15181 ttttattatg catcagttct ctgtttatag taaattatta ctcaataata cagctaataa
    15241 tgctatgata ggtcggctta aagtgaataa tcctaagaag ggtaatatca cactcttaac
    15301 agttacggaa aaacaatttg cgagaatggt ttacctccat ggagaacgca acacaagtgt
    15361 tgccaacttt gatagtcgct tggttttcct aggagattct tatgatcaag attaattttc
    15421 caattttaga tgaaccatta gtgttaagta atgctacgat tttaacgata gaagatgttt
    15481 cagtttattc ttcattggtg aaacattttt atcaatatga cgtagatgaa catttgaaat
    15541 tatttgatga taagcagaaa agtctgaagg caacagagtt aatgatggtt acagatatct
    15601 taggatacga tgtcaactca gcacctattc taaagttgat acatggtgac ttagaaaatc
    15661 aattcaacga aaagccagaa gtgaaatcaa tggtagaaaa attagcagct actattacag
    15721 aacttatcgc atttgagtgt ctagagaatg agcttgattt agaatacgat gaaatcacga
    15781 ttttagaact cattaaggca ctaggagtca aaattgagac acagagcgac actatctttg
    15841 aaaaatgttt tgaaattata caagtttacc attatttaac gaaaaagaat ctcttggttt
    15901 ttgttaatag cggagcttat cttaccaaag atgaagttat aaaattatgt gaatacatca
    15961 atttaatgca aaagtcagta ctctttctag aacctagaag actctatgat ttaccgcaat
    16021 atgttattga taaggattat ttcttgatag gcgaaaatat ggtataatat tagtaaaagc
    16081 acagtaataa caaggaatca tcgaaactga agtcctgctg agacgaatgg cgcgattacg
    16141 aaagctcaaa agaaaatttt ctacgaggtt ttagagctgt gctgtttcga atggttccaa
    16201 aactttaaat aaaaaaagcc ccacgctcaa attgttttag agctgtgctg tttcgaatgg
    16261 ttccaaaact tatgcaagct ggtgatgttg ctatttttag ttttagagct gtgctgtttc
    16321 gaatggttcc aaaacacgag ctaacggtta cctaatcagt cgccggtttt agagctgtgc
    16381 tgtttcgaat ggttccaaaa ctatcgatat tgatgattta acatcaatta tgttttagag
    16441 ctgtgctgtt tcgaatggtt ccaaaactta attaaggtat ttataccacc tttttgtgtt
    16501 ttagagctgt gctgtttcga atggttccaa aacagagtgt gtccaagacc agagttactg
    16561 tttgttttag agctgtgctg tttcgaatgg ttccaaaacc tattggttag aattttttta
    16621 caggaagaag ttttagagct gtgctgtttc gaatggttcc aaaactgcta aaaggtaaat
    16681 ttaacattcc aggtagtttt agagctgtgc tgtttcgaat ggttccaaaa ctgttttaat
    16741 catccaaaat atgttgctta agttttagag ctgtgctgtt tcgaatggtt ccaaaactct
    16801 aaaatcaagg aactgagaaa aaataaagtt ttagagctgt gctgtttcga atggttccaa
    16861 aactggtggt atttataatg tacgagcaaa tcggttttag agctgtgctg tttcgaatgg
    16921 ttccaaaacg ataaaaagtg ggagctgaat taaaaggcag ttttagagct gtgctgtttc
    16981 gaatggttcc aaaactggtg gtatttataa tgtacgagca aatcggtttt agagctgtgc
    17041 ggttattatg ctagggcacc gttgtggtgt tctagttttt tgttatactg aaataaattt
    17101 tcagagaatg tgggggaagg cggtaattag attaattcaa gacgtaattc agaacttagt
    17161 tggccaagct aacgaaatca ccccagttta tcagtttgat tgggaaactt atatattggc
    17221 gactaaaaaa tatgaacgtc atttagaggt gtgtctatta gtagaaaatt cgaattattt
    17281 ttcggattca aagagaatgt gtcgatagaa gagatacgaa agactataat tccaacctta
    17341 tggttaaagg gctagattgt tctacgcttt acttaattat tagtttgaca gcgttggttc
    17401 ttttagtgat tgctggtgtt tctttgtcac taatacttgc aagcttacaa ggaaagcagt
    17461 ggagaaccta tccataatca actaacagct atgatggatc tcacctatta atatagttaa
    17521 ggagtgtccg acactccttt tttgaaatac agttggaagt tacagaaaac atattttcat
    17581 gttataatag tggagaaaga aatgaggtgc taatattatg gaacaaacct tttttatgat
    17641 aaaacctgat ggtgtaaaac gtggttttat tggagaagtt attagtcgta ttgagcgtcg
    17701 aggcttctct attgatcgct tagaggttag acatgcagat gcagatattt tgaaaagaca
    17761 ttatgcagaa ctgacggata gaccattctt tccaacatta gtagattata tgactagtgg
    17821 tccagtaatc attggagtta tttcgggaga agaggtcatt tcaacttggc gcaccatgat
    17881 ggggagtact aaccccaaag atgcccttcc aggaactatt cgaggagatt ttgcacaagc
    17941 tccctctcca aaccaagcga catgtaatat tgttcatggg tcagactcac ctgaatctgc
    18001 agctcgcgag atagccattt ggttcaataa ctaatagact taatgaagat tgatgttcaa
    18061 gtgttaattc tcttttacat cggtcttctt tttgatataa tattctagag tctataggac
    18121 tgttttaata ttttagaagt taaacatatc aaattgtata ggaaatagaa tgaatattga
    18181 agatttgaag aaacgtcaag aaaagattag aaacttttcg attatcgctc atattgacca
    18241 tggaaaatca accttagcag accgtatttt agagaaaaca gaaacggttt ctagtcgtga
    18301 aatgcaggcg caactgttgg actctatgga cctagaacgt gagcgtggga ttactataaa
    18361 gttaaatgct attgagctta attatactgc taaagatggt gaaacttaca tttttcactt
    18421 gattgacacg ccagggcatg ttgactttac atatgaagta tcacgttcat tggcagcttg
    18481 tgagggtgct atactggttg ttgatgcagc ccaaggaatt gaagctcaaa ctctggcaaa
    18541 tgtttatttg gctctagaca atgatttaga gatactacca gttattaata aaattgattt
    18601 accagcagca gatccagaac gtgtacgtgc agaagtagag gatgttattg gtctagatgc
    18661 ttcagaagct gtacttgcct cagctaaggc agggattggt attgaagaaa ttttagagca
    18721 gatcgttgaa aaagttcctg ctccgactgg ggaagtagat gctcctcttc aagctctaat
    18781 ttttgattct gtttatgatg cttatcgtgg agtaattctg caagtaagga ttgtaaatgg
    18841 tatggttaag ccaggagata agattcaaat gatgtctaat ggtaaaacct tcgacgttac
    18901 tgaggttggt attttcactc ccaaagcagt tggacgtgat tttttagcaa ctggagatgt
    18961 agggtatatt gctgcctcaa ttaaaacagt tgctgataca cgtgttggtg ataccattac
    19021 ccttgctaat aacccagcta tagacccctt gcatggctat aagcaaatga acccaatggt
    19081 ttttgccggt ctttatccaa ttgaatcaaa taagtataac gatctgcgtg aagcccttga
    19141 aaaattacaa ttgaatgatg ctagtctaca gtttgaaccg gaaacatcac aagcattagg
    19201 gtttggtttc cgttgtggtt tcttagggct tttacatatg gacgttatcc aagagcgctt
    19261 ggaacgagag ttcaatattg atcttattat gactgcacca tcagtagttt atcatgttaa
    19321 tacaactgat ggtgaaatgc ttgaagtttc gaatccatct gaatttcctg atcctactcg
    19381 tgttgacagc attgaagaac catatgttaa ggcgcaaatt atggttcctc aagaatttgt
    19441 cggtgctgta atggagttag cacaacgtaa acgtggtgac tttgtaacaa tggattatat
    19501 tgatgataat cgtgttaatg ttatttacca aattccttta gctgaaattg tgtttgactt
    19561 ctttgataag cttaaatcat caacacgtgg ctatgctagt tttgattatg aaatttccga
    19621 atatcgtcgc tcacagttag ttaaaatgga tattctttta aatggtgata aagtggatgc
    19681 tttaagcttt attgttcaca aagaatttgc ctatgaacgt ggtaaattga tcgttgataa
    19741 gttgaaaaaa atcattcctc gtcagcagtt tgaagttcct atccaagcag ctattggaca
    19801 aaaaattgtg gctcgttctg atataaaagc actacgcaaa aatgtcttag caaaatgtta
    19861 tggtggtgac gtttcacgta aacgtaagtt gcttgaaaaa caaaaagctg gtaaaaagcg
    19921 tatgaaagca attggttccg tcgaagttcc tcaggaagct ttcttaagtg ttttatcaat
    19981 ggacgatgat gacaaaaaat aattcagaaa gatgatatca tttatctatt ttaaagtaac
    20041 ttagcttcgt gcttaagtta ctttttcttt gtctgcaatt atcaaataat a
//
LOCUS       DATRUK010000033        20039 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.033, whole genome shotgun sequence.
ACCESSION   DATRUK010000033 DATRUK010000000
VERSION     DATRUK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20039
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.033"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..1930)
                     /locus_tag="VBE34_001184"
     CDS             complement(<1..1930)
                     /locus_tag="VBE34_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEO7263906.1"
                     /translation="MEETILIVSFLLFLILSNVINRIFPKLPLPFIQLVFGILSGLVF
                     HKSQVHIDPELFLAFVIAPLNFREGQESDIGSFVKYRTIILYLILPTVFLTAIVVGYV
                     AGHLLPVSLPLAACFALGAALGPTDAVAFISIAKRFQFPKRAENILKLEGLLNDASGL
                     VSFQFALTALVTGYFSLAKASLKLALAIMGGFLIGLLFAFLMRLCLTVLEKFDAADVT
                     GALLLELTLPFVAYFVADLLGFSAIIAVVVAGVMQANRLKKVTLFDAQVDRVTSVIWE
                     TLNFILNGLVFLIFGRELIRIIGPLLTSNAYSNFDLISIVVLVTCTLFLVRFLAVSRF
                     YAWRSFKYHKSFKKYWREIQLLTFSGVKGSVSIATILLLPKHSVIGELGYSLILFTVG
                     AVTLMSFLTGLLVLPKLAPPLQVKDDYLIRLSILTKVLSVLEEDGKSSENQASFYAVI
                     DNYNSRIRHLILEQESSDIKKDLAELQLMMLSIESDGLEAAYRYGNISIKEYRIYQRY
                     LKYLERQVNRSFVSNITYSLTILMRVIRGIVHEFLTFGQGIRNRKKHFKNRKRLLAEV
                     NRQHLTDLYLSNNDLILETLEDMEGYYNATLIDFLKNERIRDAELIKSGLFVERVIAR
                     ITPDILNETLRGYYLERRT"
     gene            complement(1932..2168)
                     /locus_tag="VBE34_001185"
     CDS             complement(1932..2168)
                     /locus_tag="VBE34_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263907.1"
                     /translation="MSQLSLEERLNRLKDKYLSSKNQEFSNVHLSKVKKKKKKLIILE
                     KERCRCRLEREDTKKIDDKIEKLKKELIDVMKEE"
     gene            2335..3288
                     /locus_tag="VBE34_001186"
     CDS             2335..3288
                     /locus_tag="VBE34_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEO7263908.1"
                     /translation="MKKLTKGSHIRVLSPSSSIERLGGFEANLLAKEVLEKLGFQVSF
                     SKHYLENDILYSASIASRVEDLHEAFADPSVDAILATIGGFNSNELLPYLDYDLISKN
                     PKIICGYSDSTAFLNAIFAKAKIQTYMGPAYSSFKMKEGQPYQTQAWLTAMTENHYEL
                     WPSEEWSSDPWYDPSKPRQFFPTEWKIYNHGKASGTIIGGNLSTFGLLRGTPYAPKIE
                     RYVLLIEEAEESNFYEFDRNLAAILQAYPHPQAILMGRFPKECGMTPQVFEYILSKHA
                     IFKEIPVIYDMDFAHTQPLLTVTIGAEISVDTTTLSLSIKE"
     gene            complement(3338..5233)
                     /locus_tag="VBE34_001187"
     CDS             complement(3338..5233)
                     /locus_tag="VBE34_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HEO7263909.1"
                     /translation="MIKYQDDFYQAVNGEWAKTAVIPDDKPRTGGFSDLADDIEALML
                     STTDKWLADENKPSDTILNHFIAFHKMTADYQKREEVGVSPVLPLIEEYKGLQSFSEF
                     ASKVAEYELEGKPNEFPFGVAPDFMNAQLNVLWAEAPGIILPDTTYYSEDNEKGKELL
                     ALWRKSQEDLLPLFGLSEQEIKDILDKVLALDAKLAQYVLSREESSEYVKLYHPYNWE
                     DFTKLAPELPLDAIFQKILGQKPDKVIVPEERFWTEFASDYYSESNWELLKADLILSA
                     ANAYNAYLTDDIRIKSGVYSRALSGTPQAMDKKKAAYYLASGPYNQALGLWYAGEKFS
                     PEAKADVEHKIATMIDVYKSRLEKADWLAQSTREKAIVKLNVITPHIGYPEKLPETYT
                     KKIIDPKLSLVENATNLDKISIAYGWSKWNKPVDRSEWHMPAHMVNAYYDPQQNQIVF
                     PAAILQAPFYALEQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNNWWTDEDFK
                     AFKKLTDKVVEQFDGLESYGAKVNGKLTVSENVADLGGVACALEAAQRESDFSARDFF
                     INFATIWRMKARDEYMQMLASVDVHAPAQWRTNITVTNFEEFHKEFDVKDGDNMWRPV
                     EKRVIIW"
     gene            5381..6364
                     /locus_tag="VBE34_001188"
     CDS             5381..6364
                     /locus_tag="VBE34_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7263910.1"
                     /translation="MTEKYYNWATLGTGVIANELAQALEARGQKLYSVANRTYDKGLE
                     FANKYGIQKVYDHIDQVFEDPEVDIIYISTPHNTHISFLRKALANGKHVLCEKSITLN
                     STELKEAIDLAETNHVVLAEAMTIFHMPIYRQLKTLVDSGKLGPLKMIQMNFGSYKEY
                     DMTNRFFSRDLAGGALLDIGVYALSCIRWFMSEAPHNITSQVTFAPTGVDEQVGILLT
                     NPANEMATVSLSLHAKQPKRATIAYDKGYIELFEYPRGQKAVITYTEDGHQDIIEAGK
                     TENALQYEVADMEEAISGKTNHMYLNYTKDVMDIMTQLRQEWGFTYPEEEK"
     gene            complement(6410..7573)
                     /locus_tag="VBE34_001189"
     CDS             complement(6410..7573)
                     /locus_tag="VBE34_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7263911.1"
                     /translation="MKIFMEKLSLLSLSLILLSTFSTSPALPQMISYYRDKGLPSPQV
                     ELLFSIPSMAIIFILLITPWLSKKLSEKHMIIFGLLLTALGGGLPVVSQNYLLVFVSR
                     LLLGSGIGFINARAISVISEYYQGKERRKLLGLRGSFEVLGNAGLTALVGLLLTFGWS
                     KSFMIYFLALPILVLYLVFAPKKVVKDTNDKIKTKGQKIPKADLTYIVALAILAGFVI
                     TINTGINLRIPLLVVEFGLGTPAQASLVLSAMMLMGIIAGMSFGQLIAMFHKQLIPIC
                     LVLFSLTLLGVGLPSNLMVLTISAMASGFLYSLMVTAVFSLVADRVEYSLVGSATTLV
                     LVFCNIGGASAAILLSCFDHLLGQINAVFYVYAILSLAVGMIYFLKRLFFKKM"
     gene            7685..8329
                     /locus_tag="VBE34_001190"
     CDS             7685..8329
                     /locus_tag="VBE34_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEO7263912.1"
                     /translation="MDIENYCQDYCLETIFPKHYFKDLQVITLTAGQSVCKQGEQLEY
                     LHYIVKGRFKIVRRLFNGKEHILDIKTKPTLIGDIELLTNRQIVSSVIALEDITVIQL
                     SLKGRKEKLLTDATFLLKLSQELAQAFHDQNIKASTNLGYTVKELLASHILAIEEQGC
                     FHLELSSLADSFGISYRHLLRVIHDMVKEGLIQKEKPKYFIKNRFALESLNIQP"
     gene            8395..9009
                     /locus_tag="VBE34_001191"
     CDS             8395..9009
                     /locus_tag="VBE34_001191"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO7263913.1"
                     /translation="MSAIDKLVKASHLIDMNDIIREGNPTLRKVAEEVTFPLSEKEEI
                     LGEKMMQFLKHSQDPIMAEKLGLRGGVGLAAPQLDISKRIIAVLVPNVEDAQGNPPKE
                     AYSLQEVMYNPKVVSHSVQDAALSDGEGCLSVDREVPGYVVRHARVTIEYFDKTGEKH
                     RLKLKGYNSIVVQHEIDHIDGIMFYDRINEKNPFAVKEGLLILE"
     gene            complement(9081..10082)
                     /locus_tag="VBE34_001192"
     CDS             complement(9081..10082)
                     /locus_tag="VBE34_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7263914.1"
                     /translation="MSKKMTINDIAQLSKTSKTTVSFFLNQKFEKMSDETRQRIQEVI
                     DETGYRPSTIARSLNSKKTKLLGVLIGDITNTFSNQIVKGIEHITKQKGYQIIVGNSN
                     YDAKSEEDYIENMLNLGVDGFIIQPTSNFRKYSRILKEKKKPMVFFDSQLYEHKTSWV
                     KANNYDAVYDMTQECLNRGYKKFIMITADTSLLSTRIERASGFMDALKDNGFGYDTLV
                     IEDDDHSKSDIEDFLKAVVPDKEETLVFAPNCWALPMVFTAMKNLNFDMPRVGLVGFD
                     NIEWTDFSSPKVSTIVQPAYEEGEQVAQILINRIEGDDSVDNQQIVDCQMFWKESTF"
     gene            complement(10162..12066)
                     /locus_tag="VBE34_001193"
     CDS             complement(10162..12066)
                     /locus_tag="VBE34_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEO7263915.1"
                     /translation="MKDRLYNKFKDFDREFCQKYIKTYQSNAYQEMKASVNLMMRNTF
                     VFNDNWDMEPCSKAYCLDPLEWDNPVTDDPEWLYMLNRQTYLFKFLVVYIVEGDKSYL
                     RQMKYFMYHWIDCQFTLKPEGAVSRTIDTGIRCMSWLKVLIFLDYFGLITETKKIKLL
                     TSLREQITYMRDYYREKDSLSNWGILQTTAILACLYYYEDELNLPEIQSFAEEELLLQ
                     IKLQILDDGSQYEQSIMYHVEVLKSLMELVILAPKYYLPLEETIEKMVTYLIAMTGPD
                     YCQLAIGDSDVTDTRDILTLATLVLKSSKTKSFSFDNVNLETLLLFGKPSIYLFEEIP
                     RATIGESAYLFPDSGHVCLRDDRRYIFFKNGPFGSAHTHSDNNSVCLYDKKKPIFIDA
                     GRYTYKEEQLRYDFKRSTSHSTCTLDGQSLEMIKDSWTYNSYPKCDYCQLTSKDRYHL
                     VEGQLHVQRASDIYYHKRWLLTLPQAITLVIDKVNCPGEHVLTNQYILDDQVIYENGL
                     VNDLKLVSPTTFNLEDCLISKRYNQLTESHKLVKKIKFVDEVMDYTLIVDRNCQVKCV
                     PLVQTNSHKELSNSIAFDIRSQDFHYLIGVHMDDIIFGDKLYLMQGIKCKGKVIVYDK
                     NNGKMSRLKN"
     gene            complement(12146..12961)
                     /locus_tag="VBE34_001194"
     CDS             complement(12146..12961)
                     /locus_tag="VBE34_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO7263916.1"
                     /translation="MTGSKKLAKSDYTKTALRAFYLQNGFNYSNYQGLGYANVIYPAL
                     KKYYGDDKKALAGALEENVEFYNTNLHFLPFVTSLHLAMLDNERPEEEIRGIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLATDGLVMGPILFFVAMNTILTGIKLVTGMYGYR
                     LGTSFIDKLSEQMSVISRAANIVGVTVISSLAATQVKLTIPYTFAPEKVTSTAQKIVT
                     VQGMLDKIAPALLPALYTFLMFYLIKNKKWTTYKLVILTVIIGILGSWLGILA"
     gene            complement(12948..13814)
                     /locus_tag="VBE34_001195"
     CDS             complement(12948..13814)
                     /locus_tag="VBE34_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO7263917.1"
                     /translation="MDISILQAVLIGLWTAFCFSGMLLGLYTNRCIVLSLGVGVILGD
                     IQTALAVGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITTAGTKGKITPEAALA
                     LSTPIAVGIQFLQTATYTAFAGAPETAKKALQAGNFRGFKIAANGTIWAFAGLGFGLG
                     VLGALSTQTLTDLFALIPPVLLNGLTLAGKMLPAIGFAMILSVMAKKELIPYILLGYV
                     LAVYFGLPVLTPTANGDGVLTSVATNSVLGVPTIGVAIIATIFALLDIFRKPAAPTKE
                     TKTEGDNQDDWI"
     gene            complement(13850..14344)
                     /locus_tag="VBE34_001196"
     CDS             complement(13850..14344)
                     /locus_tag="VBE34_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEO7263918.1"
                     /translation="MAAGPNIVMTRVDERLIHGQGQLWVKFLSCNTVIVANDDVSKDH
                     LQQTLMKTVVPESIALRFFDIQKVIDIIHKANPAQTIFIIVKDLKDVYRLVAGGVPIK
                     EINIGNIHNGEGKEQVSRSIFLGMKDKEIIRKLNQEYHIAFNTKTTPTGNDGAVEVNI
                     LDYI"
     gene            complement(14399..15595)
                     /locus_tag="VBE34_001197"
     CDS             complement(14399..15595)
                     /locus_tag="VBE34_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEO7263919.1"
                     /translation="MMKIKPVKVESIENPKRFLDSRLLTKIEVEEAIEKALKQLYINI
                     DYFGEEYPTPATFNNTYKVMDNTEWTNGFWTGCLWLAYEYNQDKKLKNIAHKNVLSFL
                     NRINNRIALDHHDLGFLYTPSCTAEYRINGDAKALEATIKAADKLMERYQEKGGFIQA
                     WGELGYKEHYRLIIDCLLNIQLLFFAYEQTGDEKYRQVAVNHFYASANNVVRDDSSAF
                     HTFYFDPETGEPLKGVTRQGYSDESSWARGQAWGIYGIPLSYRKMKDYQQIILFKGMT
                     NYFLNRLPEDKVSYWDLIFTDGSGQPRDTSATATAVCGIHEMLKHLPEVDPDKETYKY
                     AMHTMLRSLIEQYSNSEFIAGRPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYK
                     DWELYW"
     gene            complement(15598..16032)
                     /locus_tag="VBE34_001198"
     CDS             complement(15598..16032)
                     /locus_tag="VBE34_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7263920.1"
                     /translation="MIKIIIVAHGNFPDGILSSLELIAGHQEYVVGINFIAGMSSNDV
                     RVALQREVIDFKEILVLTDLLGGTPFNVSSALSVEYTDKKIKVLSGLNLSMLMEAVLS
                     RTMFEHVDDLVDKVITSSHEGIVDFSTCLATQTAEATFEGGI"
     gene            16310..17122
                     /gene="dhuD"
                     /locus_tag="VBE34_001199"
     CDS             16310..17122
                     /gene="dhuD"
                     /locus_tag="VBE34_001199"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric 2-dehydro-3-deoxy-D-gluconate
                     5-dehydrogenase DhuD"
                     /protein_id="HEO7263921.1"
                     /translation="MTEQFLKDNFSLEGKVALITGASYGIGFSIATAFARAGATIVFN
                     DIKQELVDKGISAYKKLGIKAHGYVCDVTDEDGINEMVNKISQDVGVIDILVNNAGII
                     KRTPMLEMSAADFRQVIDIDLNAPFIVSKAVLPGMIQKGHGKIINICSMMSELGRETV
                     AAYAAAKGGLKMLTKNIASEYGSANIQCNGIGPGYIATPQTAPLRERQDDGSRHPFDQ
                     FIIAKTPAARWGEAEDLGAPAIFLASDASNFINGHILYVDGGILAYIGKQPQ"
     gene            17139..17777
                     /gene="dhuI"
                     /locus_tag="VBE34_001200"
     CDS             17139..17777
                     /gene="dhuI"
                     /locus_tag="VBE34_001200"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase DhuI"
                     /protein_id="HEO7263922.1"
                     /translation="MKIALINENSQASKNTLIYKELKAVSKKKGFDVFNYGMYGKEEE
                     SQLTYVQNGLLTAILLNSGAADFVITGCGTGIGAMLACNSFPGVVCGFAADSVDAYLF
                     SQVNGGNALSLPFAKGFGWGAELNLRYLFERLFEDEKGGGYPKERAVPEQRNARILSE
                     VKQITYRDLLSVLKEIDQDFLKETISGEHFQEYFFANCQNQNIADYLKSVLD"
     gene            17803..18810
                     /locus_tag="VBE34_001201"
     CDS             17803..18810
                     /locus_tag="VBE34_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO7263923.1"
                     /translation="MSKILFFGEPLIRITPKENDYFADSISTKLFYGGSEVNIARALQ
                     GFGQDTKLLSALPNNPIGNSFLQFLKAQGIDTHSIQWVGERVGLYFLENSFACRKGEV
                     VYDRDYSSLHDFRINQIDFDQLFEGVSLFHFSGITLSLDESIQEITLLLLKEAKKREI
                     TISLDLNFRSKLISPKNAKILFSQFATFADICFGIEPLMVDSQDTTFFNRDEATIEDI
                     KERMISLINHFDFQVIFHTKRLQDEWGRNHYQAYIANRKQEFVTSKEITTAVNQRIGS
                     GDAFVAGALYQLLQHSDSKTVIDFAVASASLKCALEGDNMFETVTAVNKVLNQSKDII
                     R"
     gene            18822..19460
                     /locus_tag="VBE34_001202"
     CDS             18822..19460
                     /locus_tag="VBE34_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO7263924.1"
                     /translation="MIKSKILLQLMQGKIVAVIRGNSQEEAFQAAQACIKGGISAIEI
                     AYTNSKASQVIEQLVTQYTNQEQVVVGAGTVLDAETARMAILAGAKFIVSPAFNLQTA
                     KLCNRYAIPYLPGCMTLSEITTALEAGCEIIKIFPGGTLGTSFISSLKAPLPQVQIMV
                     TGGVNLTNAKDWFLSGVTAIGIGGEFNKLAALGEFDKITEMAKQYKIQLNLN"
BASE COUNT         6547 a         3904 c         3254 g         6334 t
ORIGIN      
        1 ttgttctgcg ttccaagtaa tatcctctta gggtttcatt taaaatatct ggcgttatgc
       61 gtgcaatgac acgttctaca aataaacctg atttaattaa ttctgcgtct cgaatgcgtt
      121 cattttttaa gaaatctatc aaagttgcat tataataacc ttccatatct tctagcgttt
      181 ctaaaatgag gtcattattt gataagtaca aatctgttaa gtgctgacga ttgacttctg
      241 ctaacaaccg tttcctgttt ttgaaatgtt ttttccgatt tcgtattcct tgtccaaatg
      301 ttaaaaactc atgaacaata ccacgaataa cacgcataag aatcgttaaa ctataagtga
      361 tatttgacac aaagctacga ttaacttgac gttctaagta ttttaaataa cgctggtaaa
      421 tacgatattc tttgatagaa atattgccat aacgataagc agcttcaagc ccgtcgcttt
      481 ctatactaag catcatcagt tgcaattcag ctaagtcctt cttaatgtca ctagattctt
      541 gttctaaaat gagatgacga atacggctat tataattatc tataactgca taaaatgaag
      601 cttgattttc agaactctta ccgtcttctt ctaagacaga taatactttt gttaaaatcg
      661 atagacgtat taaataatca tctttcacct gtaagggagg cgctaacttt ggtaatacta
      721 aaagtcccgt taaaaaactc attaacgtta ctgcaccaac agtgaaaaga atcaaactat
      781 accccaactc tcctatgact gagtgcttgg gtaataagag tattgttgct atggaaacac
      841 ttcctttaac tcctgaaaaa gtcaacagtt gaatctctct ccaatacttt ttaaatgatt
      901 tatggtattt gaaagatctc caagcataaa agcgagaaac tgctagaaag cgaactagaa
      961 aaagtgtgca agtaactaaa acaacaatac taataagatc aaaattacta taagcgttac
     1021 ttgttaaaag aggaccaatg atacgtatca gttctcttcc aaatattaag aaaactaaac
     1081 catttaaaat aaaatttaag gtttcccaaa taacagaggt aacgcgatct acttgagcat
     1141 caaagagagt cacttttttc aaacgatttg cttgcattac tcctgccact accaccgcaa
     1201 taatagctga aaatcccaac aaatcagcaa cgaaataagc aacaaaaggt aaggttaatt
     1261 ccaaaagtag agcccctgta acatcagcag catcaaattt ctctaggaca gtgagacata
     1321 acctcattag aaaagcaaaa agcaagccaa ttaaaaaacc acccattatg gccaatgcta
     1381 acttgagact agcttttgct aaggagaaat aacctgttac aagggctgtt aaagcaaact
     1441 ggaaggaaac tagaccacta gcgtcattta aaagaccttc caatttgagg atattctctg
     1501 ctcgtttagg aaattgaaac ctcttagcaa tcgatataaa ggcaacggca tctgttgggc
     1561 ctaaggctgc tccaagcgca aagcatgccg caagtggtaa actaactggc aaaaggtgcc
     1621 ctgctacata accaacgaca atagctgtta aaaataccgt tggtaagatt aagtaaagaa
     1681 taatagtccg atactttaca aaactaccaa tatcactttc ttgaccttcc ctaaagttca
     1741 aaggagcaat aacaaaggca agaaataact caggatcgat atggacttgt gacttatgaa
     1801 aaactaagcc agagagaatc ccaaagacca actggataaa tggtaatggt aatttaggaa
     1861 atatcctatt gataacatta gataaaatca aaaacaggag aaagctgact attagaatag
     1921 tttcttccat attattcctc cttcataaca tctatcaatt ctttcttcaa tttctcaatt
     1981 ttatcatcga tttttttagt atcttctctc tccaatcgac agcgacatct ttctttttca
     2041 agtattatta atttcttctt tttcttcttt actttactta agtgaacatt tgaaaattct
     2101 tgatttttag aagaaaggta tttatctttt agtcgattca atcgttcttc caaagataat
     2161 tgagacatca tgacctccta tttaataacc aaattattaa taataagtat tttttctttc
     2221 atcggctatc tctagtataa cacgcatttc ttaaattgat agaatttaag aatatcgttg
     2281 ctcattatct ccatatgtgg tagactaaca acaatttaaa taggaggtta accaatgaaa
     2341 aaattaacaa aaggcagcca cattcgtgtt cttagtccct cttcttccat tgaacgtctg
     2401 ggtggttttg aggctaacct attagccaaa gaagtactag aaaagctggg atttcaagtg
     2461 tcattctcta agcactatct agaaaatgat atcctctatt ctgcttcgat tgctagccgc
     2521 gtagaagatt tacatgaagc atttgccgac ccttctgttg atgccatatt agcaaccatt
     2581 ggcggattca attccaacga gcttttacca tatttagatt atgatttaat ctctaagaat
     2641 cccaaaataa tttgtggtta ctcagattca accgccttcc ttaatgctat ttttgccaaa
     2701 gcaaaaatcc aaacttacat gggccctgct tattcttctt tcaaaatgaa ggaaggtcaa
     2761 ccttaccaaa cacaagcttg gcttactgcc atgacagaaa accactatga actttggcct
     2821 agtgaagaat ggtctagtga tccctggtat gatcccagca aaccacgcca atttttccca
     2881 acagaatgga aaatttataa tcacggaaaa gcttccggta cgattatcgg aggtaatctc
     2941 tctacgtttg gcctacttcg aggtacccct tatgcaccga aaattgaacg ctatgtcctc
     3001 ttgattgaag aggctgagga aagtaatttt tatgaatttg accgcaacct tgctgcaatt
     3061 ttacaagctt atcctcatcc acaagctatt cttatggggc gctttcctaa agaatgcggc
     3121 atgactccac aagtctttga atatatcctt tcaaaacatg ctattttcaa ggaaattcct
     3181 gttatttatg atatggattt tgcgcacacc caacctcttt tgacagtgac tattggagct
     3241 gagataagcg ttgacactac tacattatct ttatctataa aagaataaaa aaatactcct
     3301 gctactcatc tgagtaacgg gagtttatca tttagagtta ccaaataata acacgttttt
     3361 caactggacg ccacatgtta tcaccatctt taacatcaaa ctctttatgg aattcttcaa
     3421 aattcgttac tgtaatatta gttcgccatt gtgctggggc gtgaacatct acactagcta
     3481 acatttgcat atactcatca cgtgccttca tacgccaaat tgtcgcaaag ttaataaaga
     3541 aatccctcgc tgaaaaatct gactcacgct gcgcagcttc taaagcacaa gcaactccac
     3601 ctaaatctgc aacattttct gatactgtta gtttaccatt gacttttgcg ccataagatt
     3661 ccagtccatc aaactgctca actactttat ctgtaagttt tttgaaagct ttaaaatctt
     3721 catcagtcca ccaattattc aaacttccat gttcatcaaa tgaagctcca ttagtgtcaa
     3781 aagcgtgtga tatttcatgt gcaatcactg caccaattcc cccataattt gctgatgaac
     3841 tttgctctaa agcataaaat ggtgcttgta aaatggctgc tgggaagaca atttggtttt
     3901 gttgtggatc ataatacgca ttgaccatgt gggcaggcat atgccattca cttctatcaa
     3961 ctggtttatt ccatttactc caaccatagg ctattgaaat tttgtctaaa ttagtagcat
     4021 tttcgacaag cgatagttta ggatcaatga tttttttagt ataagtctct ggtagtttct
     4081 cagggtatcc aatgtgtggc gtgataacat taagtttcac aatagctttt tcccgtgttg
     4141 actgtgcaag ccaatccgcc ttttctaagc gtgacttata gacatcaatc atcgttgcaa
     4201 tcttatgctc aacatcagct ttagcttctg gagagaattt ttcccctgca taccacaaac
     4261 ctagagcttg attgtaaggt cctgaagcaa ggtagtaagc tgcttttttc ttatccattg
     4321 cttgtggtgt gcctgataaa gctcgactgt aaactcccga tttgatacga atatcatcag
     4381 ttaagtaagc attataggca ttcgcagctg ataaaataag atctgccttt aataattccc
     4441 aattagattc tgagtaatag tcagatgcaa attctgtcca gaaacgttct tcaggaacaa
     4501 taactttatc tggtttttgt cctaatatct tttggaaaat agcatctaat ggaagttcag
     4561 gtgctaattt tgtgaaatct tcccagttat aaggatggta aagtttaaca tattccgaac
     4621 tctcttcgcg agataagaca tattgtgcca attttgcatc caaagctaaa accttatcca
     4681 agatatcttt aatttcctgt tctgaaaggc caaacaaagg cagtaagtct tcttgacttt
     4741 tacgccaaag agcaaggagt tcctttcctt tttcattatc ttcactataa taagttgtat
     4801 ccggtaaaat aatcccaggc gcttcagccc aaagtacatt caattgagca ttcataaagt
     4861 caggagccac accaaatgga aattcatttg gtttaccttc tagttcatat tctgcaactt
     4921 ttgatgcaaa ctcagagaaa gattgtagac ctttatattc ttcaattaat ggtaatactg
     4981 gtgatactcc tacctcttca cgtttttgat aatcagctgt cattttatga aaagcaataa
     5041 aatgatttaa aatcgtatca cttggtttat tttcatcagc cagccactta tctgtcgttg
     5101 acagcattaa tgcttcaatg tcatcagcaa gatcagaaaa accacctgtc cttggtttgt
     5161 catcaggaat aacagctgtc ttagcccatt ccccattaac cgcttggtaa aaatcatctt
     5221 gatattttat cattattttc tccttaatat ctcatatgta atctcttaca gtctaacaga
     5281 aaattcagag tttgactatt aaaaatactc tcatattggt gctttcttta aatatcattt
     5341 tgttattttt gctatactat aaaccaacaa ggaggttgtt atgactgaaa aatattataa
     5401 ttgggcaacg cttggaaccg gcgttattgc caacgaatta gcccaagcac tggaagcacg
     5461 tggacaaaaa ttatattctg tagctaatag aacttacgac aaaggacttg aatttgctaa
     5521 caaatatggt atccaaaaag tttatgatca catagatcaa gtatttgaag accctgaagt
     5581 ggatatcatt tatatctcta ctccccacaa tactcacatc tcatttttac gaaaggcttt
     5641 agcaaatggt aagcacgttc tttgcgaaaa atctattact ttaaatagta ctgagcttaa
     5701 agaagccata gatttagccg aaactaacca tgttgtctta gctgaagcca tgactatttt
     5761 tcatatgcca atttaccgcc aattaaaaac attagttgat agtggaaaat taggaccgtt
     5821 aaaaatgatt caaatgaatt tcggaagtta taaagaatat gatatgacta accgtttttt
     5881 cagtcgtgac ctagcaggcg gtgctttgct ggacattggt gtttatgcac tttcttgtat
     5941 tcgctggttt atgtcagaag cacctcacaa cattacctct caagttacat ttgcaccaac
     6001 aggggttgat gaacaagttg gtatcctact aaccaaccca gcaaatgaga tggcgactgt
     6061 cagccttagt ttacatgcaa aacaacctaa acgagcaact atcgcttacg ataaaggcta
     6121 cattgaactt tttgaatatc cgcgaggaca aaaggcagtt attacttata ctgaggatgg
     6181 gcatcaagat attatcgaag ctggcaaaac tgaaaatgct ctccaatatg aggtagctga
     6241 tatggaagaa gccatttcag gaaaaactaa ccacatgtac ttaaactata ccaaagatgt
     6301 tatggatatc atgacacagc tacgtcaaga atggggattt acctacccag aagaagaaaa
     6361 atgaagagag cttgacaatg attgtcaagc cctttttagt taatatacac tacattttct
     6421 taaaaaatag gcgtttaagg aaataaatca taccaactgc taaacttaaa atagcataaa
     6481 catagaaaac tgcatttatt tgtcctagta aatgatcaaa acaagacaga agaatagccg
     6541 cactagctcc accaatatta caaaatacta aaactaatgt cgttgcagaa ccaaccaacg
     6601 aatattcaac acggtctgct actaggctaa acacagcagt gaccatcagg ctgtaaagga
     6661 aacctgacgc catcgcacta attgttagta ccattagatt agaaggcaat ccaactccaa
     6721 gcaatgttaa agaaaataag acgaggcaga ttggaattaa ttgtttatga aacatagcta
     6781 tcaactgacc aaaactcata cctgctatta ttcccataag catcatcgca ctaagaacaa
     6841 gactagcttg agctggcgta cccaaaccaa attctactac tagcagaggt atcctaagat
     6901 taatcccagt atttatagtt atgacaaatc ctgctaaaat agctaaggcg acaatataag
     6961 tcaaatcagc tttagggatt ttttgacctt tagttttaat cttgtcattt gtatctttca
     7021 ccactttctt aggcgcaaaa acaagatata gaactaagat tggtaaagct aaaaaataaa
     7081 tcataaatga tttagaccaa ccaaaagtca acaatagccc aactaaggct gtcaaaccag
     7141 catttcctaa aacttcaaag gaccccctta atcctaagag ttttcttctt tctttgcctt
     7201 gataatattc actaatcaca ctaatggccc tagcattaat aaaaccgata ccactcccca
     7261 ataaaagacg cgatacaaat actaaaaggt agttctgact gacaaccggc aatcctcccc
     7321 ctaatgctgt tagcaatagt ccaaagataa tcatatgttt ctcagaaagt tttttactta
     7381 accaaggtgt gataagcaag atgaagataa tagccatcga aggaattgaa aataataatt
     7441 caacttgggg tgatggtaag cccttatctc tataataaga tatcatttgt ggtagggctg
     7501 gtgatgtcga aaaagttgat agcaagataa gtgataaaga gagtaaactt agcttctcca
     7561 taaagatttt caagtgacaa tacctctttc tgatgtcttt ttttagataa ataaagtata
     7621 atcaatatta aactagaaat atatgacata tgtcctaaaa ctatctcaaa tagggagtca
     7681 atacatggat atagaaaact actgccaaga ctactgtttg gagactatct ttccaaaaca
     7741 ttatttcaag gatttacaag taatcaccct aactgccggc caatccgtct gtaaacaggg
     7801 agaacaatta gaatacttgc actacatcgt caagggacgt tttaaaattg tacgtcgact
     7861 ctttaacggc aaagaacata ttttagatat taaaacaaaa ccaacactga ttggtgatat
     7921 tgaacttttg actaatagac aaattgtatc atctgttatt gccttagagg atattacagt
     7981 cattcaatta tcacttaaag gacggaaaga gaaactgtta acagacgcaa ctttcctcct
     8041 aaaacttagt caagagctag ctcaggcttt tcatgatcaa aacatcaaag cgtctactaa
     8101 tttagggtat accgtcaaag agttacttgc aagtcatatt ttagcaattg aagaacaagg
     8161 ttgctttcac cttgaactaa gtagtttagc tgattcgttt ggcatcagtt atcgtcattt
     8221 attaagagtt atccatgata tggtcaaaga aggccttatc caaaaagaaa aaccaaaata
     8281 ctttatcaaa aatcgtttcg cactagaatc attaaatatt cagccatgaa gcgtagcttt
     8341 atcatttaat tattttatgc taaactaaat cagagaatac agttaggaga aaatatgtct
     8401 gctattgata aattagtaaa agctagtcat ttaattgaca tgaatgacat tatccgcgaa
     8461 ggcaatccaa cccttcgcaa agtggctgaa gaagtcacat tcccactttc tgagaaagaa
     8521 gaaattctcg gagaaaaaat gatgcaattt ttaaaacact ctcaagatcc aattatggct
     8581 gagaagctag gcttacgtgg tggtgttggt ttagccgcac cacaattaga catctcaaaa
     8641 cgcatcattg ccgtccttgt tccaaacgtt gaggatgctc aaggaaatcc acctaaagaa
     8701 gcttatagcc tccaagaagt aatgtacaac cctaaagttg tatcgcattc tgttcaagat
     8761 gctgctttat ctgacgggga gggatgccta tcagtagatc gtgaagtacc tggctatgtc
     8821 gtccgccacg cgcgtgtaac tattgaatat ttcgacaaaa ctggtgaaaa acatcgactc
     8881 aaattaaaag gttacaactc tattgttgtt caacatgaaa ttgaccacat tgatggtatt
     8941 atgttttacg accgtatcaa tgagaaaaat cctttcgctg tcaaagaagg cttacttatt
     9001 ttagaataag gacaaataac ctagagataa ttttctctag gttttatttt ttaatagcaa
     9061 ataaaataag aaatcttttt ttaaaaagtc gactctttcc aaaacatctg gcaatctaca
     9121 atctgttggt tatcgacgga gtcatctcct tcaatacgat taatcaaaat ctgtgctact
     9181 tgttcacctt cttcataagc cggctggaca attgttgaca ctttaggaga tgaaaagtct
     9241 gtccattcaa tattgtcaaa cccaactaaa ccaactcgtg gcatatcaaa attgagattt
     9301 ttcatggcag taaaaaccat tggtaaagcc caacaatttg gtgcaaatac taatgtctcc
     9361 tccttatctg gaactaccgc ttttagaaag tcttctatat cactttttga atgatcatca
     9421 tcttcaatca ccaaagtgtc ataaccgaaa ccattgtcct ttaaagcatc catgaatccc
     9481 gaagctctct caatacgtgt actaagcaag ctcgtatcag cagtaatcat aataaacttt
     9541 ttataccctc gatttagaca ctcctgtgtc atatcataaa cagcatcata gttattagcc
     9601 ttaacccaac tcgtcttatg ttcataaagt tgactatcaa aaaataccat tggtttcttt
     9661 ttttctttta aaatacgaga atactttcga aaatttgatg taggctgaat aatgaaacca
     9721 tcaacaccta aatttaacat attttcaata taatcttctt cacttttggc atcataatta
     9781 ctattaccca caataatttg atagcctttt tgtttggtga tgtgctcaat tccttttaca
     9841 atttgatttg aaaatgtgtt tgttatatct ccaattaaga cacctagtag ttttgttttt
     9901 ttagaattta gacttcttgc tatcgtactt ggtctataac ctgtttcatc aatcacctct
     9961 tgaatacgct gcctcgtctc atccgacatt ttttcaaatt tttgattcaa gaaaaaagaa
    10021 accgttgttt tggaagtttt tgataattga gcgatgtcat taatggtcat ttttttagac
    10081 ataacaaacc tcctttctaa ttttactgat tttatttttt tatggttaat atagtactag
    10141 tatagcataa taatagccct cttaattttt taaacgactc attttgccat tatttttatc
    10201 atatacaatg acttttcctt tgcattttat cccttgcatc aaatagagtt tatccccaaa
    10261 gataatatca tccatatgca ctccaattaa ataatgaaag tcttgagacc taatatcaaa
    10321 tgcaatgcta ttacttagtt ccttatgact gttcgtttgc actaaaggaa cacatttcac
    10381 ctgacagttc cgatcaacta tcaaggtata atccatcacc tcatcaacaa attttatttt
    10441 cttaactaat ttatgacttt ctgtcaattg attataccgc ttagaaataa ggcaatcttc
    10501 tagattaaag gtcgtaggac ttactaattt caagtcgtta acaagcccat tttcataaat
    10561 gacctgatca tctaaaatat attgatttgt taaaacatgc tctcctggac aattcacctt
    10621 atcaataact aaggtaatgg cctgcggtaa agttaacaac catcgcttat ggtaatagat
    10681 atcagaagct ctttggacat gtagttgtcc ttcgactaaa tggtacctat cctttgaagt
    10741 cagctgacaa tagtcacatt ttggataaga attgtatgtc caagagtcct tgatcatttc
    10801 taaagattgc ccatcaaggg tacatgttga atgactagtc gaacgtttaa aatcatacct
    10861 tagttgttct tctttgtaag tatatcttcc tgcatcaatg aaaataggtt tctttttatc
    10921 atagagacaa acactattat tatcactatg agtatgagcg ctaccaaatg gaccgttctt
    10981 aaaaaatata taacgcctat catcacgtaa acacacatga ccagaatctg gaaaaagata
    11041 agcagactca cctattgttg cacgcggtat ttcttcaaaa agataaattg atggcttccc
    11101 aaacaaaagt aaagtttcta aattaacatt atcaaaagaa aatgattttg ttttagaaga
    11161 tttcaacaca agtgttgcca aagttagaat atcacgagta tcggtcacat cactgtcccc
    11221 tatagctagt tgacaataat ccggaccagt catagcaatt agataggtaa ccattttttc
    11281 aatagtttct tctaatggta gataatattt aggcgcaagg ataactagtt ccattaagga
    11341 cttcaagact tctacatgat acataattga ttgttcatac tggctcccgt catctaaaat
    11401 ttgaagcttg atttgaagta ataattcttc ttcagcaaaa ctctgaattt caggtaggtt
    11461 taattcatct tcataatagt ataaacacgc caatattgct gttgtttgta aaattcccca
    11521 gttacttaga ctatcttttt cacgataata gtccctcata taagttatct gctcccgtaa
    11581 actagttaat agtttaattt ttttagtttc tgttattaat ccaaaataat ctaaaaaaat
    11641 caaaactttg agccatgaca tgcaacgtat cccagtatca atagtacgac ttaccgcccc
    11701 ttctggcttt agtgtaaatt ggcaatcaat ccaatggtac ataaaatatt tcatttgtct
    11761 aagataagac ttatcgcctt caacaatgta gaccaccagg aatttaaaaa gataagtttg
    11821 acgatttagc atatacaacc attctggatc atctgttact ggattatccc actccaaagg
    11881 atcaagacaa tatgctttag aacaaggttc catatcccaa ttatcattaa atacaaaagt
    11941 gttcctcatc atcaaattaa cagaggcttt catttcttga taggcattag attggtatgt
    12001 tttaatatac ttttgacaaa attccctatc aaaatccttg aatttattat ataagcgatc
    12061 tttcattttt ttacaaaacg tcctcgtccg atataaaaag gactatgtca ttgatatatc
    12121 ataatcctta tttatcaaaa ttttattaag ctaaaatacc taaccagcta ccaaggatac
    12181 caataataac cgtaaggata actagcttat aagttgtcca ttttttattt ttaataaggt
    12241 aaaacataag gaaagtgtag agtgctggaa gtagagctgg tgcaatttta tcaagcatac
    12301 cttggacagt aacaattttt tgcgctgtac tagttacttt ttctggagca aaagtgtaag
    12361 ggattgtaag cttaacttgt gttgcagcta aacttgagat gaccgttaca ccaacgatat
    12421 tagctgcccg tgagataact gacatttgtt cactcaattt atcaataaaa ctggtcccaa
    12481 gacgataacc atacatgcca gtaactaatt taattcccgt taaaatagta ttcatagcca
    12541 caaagaacag aatcggtccc atcaccaaac catctgtcgc taaagaggct gcaatcgttg
    12601 agaacaaggg tgctaagcaa aattgtgata aagaatcacc gatacctgca agaggaccca
    12661 taagcgccat cttaataccg cgaatttctt cttctggccg ctcattatca agcattgcta
    12721 aatgtaaact agttacaaaa ggtaagaaat gaaggtttgt attgtaaaac tccacatttt
    12781 cttctagagc tcccgctaaa gcttttttgt catctccata atattttttg agagctggat
    12841 atatgacatt agcataacca agaccttgat agttactgta attaaagcca ttttgtaaat
    12901 aaaaggcacg taaagctgtt ttagtgtaat cactttttgc taatttttta gatccagtca
    12961 tcttggttgt ctccttctgt cttagtttct ttagtaggtg cagctggttt tctgaaaata
    13021 tcgagcaaag caaatattgt tgcaattata gctaccccga tagttggaac cccaagcaca
    13081 ctgttagtag ccactgatgt taaaactccg tctccatttg ctgttggagt tagaactggc
    13141 aaaccaaaat aaacagctag aacatatcca agtaggatat aaggaatgag ttctttttta
    13201 gccattacag agagaatcat cgcaaaacca attgcaggaa gcattttccc agctaaggtt
    13261 agaccattaa gcaaaacagg gggaatgaga gcaaataaat ctgtcaaagt ttgcgttgaa
    13321 agagcaccca gtactcctaa accaaatccc aatccagcga atgcccaaat tgtaccattt
    13381 gctgcaattt taaatcctcg gaaattacct gcttgcaaag cttttttagc tgtttcaggc
    13441 gcacctgcaa aagcagtata agttgctgtt tgaagaaatt gaataccaac tgcaattggg
    13501 gttgataatg caagagcagc ttccggtgtg atttttcctt tagttccagc tgttgtaata
    13561 gccatcaaag ttccaaaaat cccaggaccg attgggtttg gaggaactgt ccctcccgct
    13621 cccacaccaa atcccatgta agccagttca gatattgctc caactgccaa agctgtttga
    13681 atatctccta aaataacacc aacgcctaga gagagaacga tacaacgatt tgtatagagg
    13741 ccaagcaaca tgccactaaa acagaaagcc gtccacagtc caattaagac agcctgaaga
    13801 atactaatat ccatattttt ctcctttata tgatagtgag tttgtccaac taaatataat
    13861 ctaaaatatt aacttccaca gcaccatcgt tacctgttgg agttgtttta gtattaaatg
    13921 caatgtgata ttcttggttt aattttctaa ttatctcttt atccttcatc cctaagaaga
    13981 tagagcgact tacctgctct ttaccttctc cattatgaat atttccaata ttaatctctt
    14041 taattgggac gcctcctgct acaagtctat agacatcttt caaatcttta acaattataa
    14101 aaatcgtctg tgctgggttg gccttgtgaa taatatcaat aactttttga atatcaaaaa
    14161 atcgcaatgc aattgattct ggcaccaccg tcttcattaa cgtttgttga agatgatctt
    14221 tggagacatc atcatttgcc acaataacag tattacaact taaaaatttc acccaaagtt
    14281 gtccttgtcc atgaataaga cgctcatcaa cacgtgtcat tacaatattt ggtcctgctg
    14341 ccatttctga cctccttgtt ttctattatt tattgtttat tttgttagta aaatactctt
    14401 accaataaag ttcccagtct ttatagaatc ttattaaggc ttctaagtaa taataatctc
    14461 cccaaatatt accttcatct actcctttac ctgaatgcca cgaatacaca ccgtgcaata
    14521 gaagaggacg tcctgctata aattcactat tactatactg ttcaatcaga ctacgaagca
    14581 ttgtatgcat agcatatttg tatgtctctt tatcaggatc tacttctggt aaatgtttaa
    14641 gcatctcatg aatcccacac acagctgttg ctgttgcgga tgtatctcta ggctgacccg
    14701 agccatccgt aaaaataagg tcccaatagg ataccttgtc ttcaggtaaa cgatttagaa
    14761 aatagtttgt cataccttta aaaaggataa tctgctgata atctttcatt ttccggtaac
    14821 taagcggaat accgtagatg ccccatgctt gccctcttgc ccaagatgac tcatcactat
    14881 aaccctgtcg tgtgacacct tttaacggtt ctccagtttc tgggtcgaaa taaaaagtat
    14941 gaaaagcaga agaatcatca cgcaccacat tgttagctga agcgtagaag tgattcaccg
    15001 caacttgtct atacttttca tcacctgtct gttcataagc aaaaaataag agttggatat
    15061 taagtaagca atcgataatt aagcgatagt gttccttata cccgagttct ccccaagcct
    15121 gaataaatcc acctttttct tgatagcgct ccatcaattt atctgcagct tttatagtgg
    15181 cttctaaagc tttagcatca ccattgatac gatattctgc tgtacaagat ggtgtgtaaa
    15241 gaaatcctaa gtcgtggtga tctaatgcta tacgattatt aatacgattt agaaatgaca
    15301 atacattttt gtgggctatg ttttttaact ttttatcctg attatactca taagctaacc
    15361 acaagcaccc tgtccaaaaa ccatttgtcc attctgtgtt atccataact ttataagtat
    15421 tattgaatgt tgcaggcgtt ggatactctt caccaaagta atcaatatta atataaagtt
    15481 gcttcaaggc tttttcaatg gcttcctcaa cttcaatctt agttaataat ctactgtcta
    15541 aaaaacgctt tggattttca attgactcaa ccttgaccgg ttttattttc atcattatta
    15601 aatgcctccc tcaaaagttg cttctgcagt ttgtgtagca aggcaagtag aaaaatcaac
    15661 aattccttca tgagatgatg ttataacttt atcaacaagg tcatctacat gttcaaacat
    15721 cgttctagat aaaacagctt ccattaacat tgaaaggttc aagcccgata aaacttttat
    15781 ctttttatct gtatattcta ctgataaggc ggaagaaaca ttgaaaggag tcccacctag
    15841 caaatcagtc aatactaaaa tttctttgaa atcgataacc tctctttgca aagcaactct
    15901 aacatcatta gaagacatgc ctgcaataaa gttaatacca acaacatatt cctgatgccc
    15961 agcaataagt tctaaagagc ttaaaatacc atctggaaag ttgccgtgtg ctacaataat
    16021 aatttttatc atattttctc ctcttgagtt taccggttta cttttataat cagattatag
    16081 cgctttcatt tttaggtgtc aacatctttt ttatattttt tgagcagttt acttgatttt
    16141 ttataatgat gtctttttta tctttaagaa tcataactta gataaagctt tgttatatat
    16201 gttgccaagt tttataggag aagaactcta tagttgacaa aatcggttta ctttgttact
    16261 attcatgtaa acaggtttac ttcctgaatt tttttacgga ggtcatatta tgactgaaca
    16321 attcttaaaa gacaactttt cactagaagg taaagtcgcc ttaataacag gcgcttctta
    16381 cggcattggt ttttcaatag caactgcttt tgctagagct ggagcaacta ttgtcttcaa
    16441 tgatattaag caggaactcg ttgacaaagg catctccgcc tataaaaaac ttggcattaa
    16501 ggcccatggc tatgtttgcg atgttacaga tgaagatgga atcaacgaaa tggtgaacaa
    16561 aatttctcaa gatgttgggg taattgatat tctagttaac aacgcaggta ttataaaacg
    16621 cacccctatg ctagagatga gcgctgcaga ttttcgacaa gttattgata ttgatttgaa
    16681 tgctcccttt atcgtatcaa aagcggtatt acctggaatg atccaaaaag gacatggtaa
    16741 aatcatcaat atttgctcca tgatgagtga gcttggacgc gaaacagttg ctgcctatgc
    16801 tgctgccaaa gggggactta aaatgttaac caagaacatt gcttctgaat acggttctgc
    16861 taacatccaa tgtaacggta tcgggcccgg ctatattgcc acaccacaaa cagctcccct
    16921 tcgtgaacgt caagatgatg gttcacgtca tccattcgac caatttatca tcgctaaaac
    16981 acctgccgct cgctggggtg aggcagaaga tttaggtgca ccagctatct tccttgctag
    17041 cgatgcatca aactttatca atggtcacat actctatgta gatggcggta ttttagctta
    17101 catcggtaaa caacctcaat aataaaggag taatataaat gaaaattgca ttaatcaacg
    17161 aaaatagcca agcgagtaaa aatactctta tctacaaaga attgaaagcg gtttccaaaa
    17221 aaaaaggatt tgatgttttc aactacggga tgtatggcaa agaagaagaa agccagctaa
    17281 cttatgtaca aaatggttta ctgactgcta ttttacttaa tagcggtgct gcagattttg
    17341 ttattacagg ctgtgggaca ggaattggag cgatgcttgc ttgtaatagc tttcctggcg
    17401 tagtctgtgg attcgctgct gattctgtcg acgcttactt attctctcaa gtaaacggag
    17461 gaaatgctct ttcccttcct tttgctaaag gatttggttg gggagctgaa ttgaatctga
    17521 ggtatttatt tgaacgtctt ttcgaagatg aaaaaggagg gggataccct aaagagaggg
    17581 ctgtacctga gcaacgcaat gcacgtatcc ttagcgaagt aaaacagatt acttacagag
    17641 atttactctc tgttttaaaa gaaattgatc aagatttctt aaaggaaact atttctgggg
    17701 agcatttcca agaatatttc ttcgctaatt gccaaaatca aaatatagca gactacttga
    17761 aatctgtctt agactaaagc gtctgtttta gaaaggactt ttatgtctaa gattcttttt
    17821 ttcggtgaac ctctcattcg catcactccc aaagaaaatg actattttgc agatagtatc
    17881 tcaactaaac tattctatgg tggctctgaa gttaatatcg caagagctct acaaggcttt
    17941 gggcaagata ctaaactcct ctcagcctta cccaataatc ccataggtaa tagttttctt
    18001 caatttttaa aagcacaggg tattgatact cattctattc agtgggttgg agaacgtgtt
    18061 gggttatatt tcttagaaaa ttcctttgct tgcagaaaag gtgaggtggt ctacgaccgc
    18121 gattatagta gtttacatga ctttaggata aaccaaattg atttcgatca attatttgaa
    18181 ggagtttctc tctttcattt tagtggtatt acactatcat tggatgagtc tatccaagag
    18241 ataacactgc ttcttttaaa agaagctaaa aaaagagaga taacaatctc cttagatctc
    18301 aattttcgca gtaaactaat ttctcctaaa aatgccaaaa tccttttttc tcagttcgct
    18361 acctttgcgg atatttgttt tggtattgaa cctcttatgg ttgactcaca ggacactacc
    18421 ttcttcaatc gcgatgaagc tacaatagag gacatcaaag aacgaatgat ttctcttata
    18481 aatcactttg actttcaagt cattttccat actaaacgtc tacaggatga atggggacga
    18541 aaccactacc aagcttacat agcaaatcgt aaacaagagt ttgtcacttc aaaagaaatc
    18601 accacagctg taaaccagag aattggaagc ggagatgctt ttgtggcggg agcgctatat
    18661 caactacttc agcactcaga cagcaagaca gtcattgatt tcgctgtcgc tagtgcatct
    18721 ttaaaatgtg ctttggaagg ggacaatatg tttgagaccg ttaccgcagt taacaaagta
    18781 ctaaatcaat cgaaagacat tattcgttag aagggagaat tatgataaaa tcaaagattt
    18841 tgttgcaatt aatgcaagga aaaatagttg ctgtcattcg tggaaatagc caagaagaag
    18901 cttttcaagc tgctcaagca tgcataaaag gtggtatttc tgctatcgaa atcgcctata
    18961 ctaatagcaa agctagccaa gtcattgaac aactagtcac acaatatact aaccaagaac
    19021 aagttgtcgt tggggcagga actgttctag acgcagaaac tgcccgaatg gctattttag
    19081 caggagcaaa gtttattgta tctcccgcat ttaatctaca gactgctaaa ttatgtaatc
    19141 gttatgctat tccttattta ccaggttgca tgaccttatc agagataaca acagcacttg
    19201 aagctggttg tgaaataatt aaaatattcc caggaggtac tctaggaaca tctttcatct
    19261 catcactaaa agcaccttta cctcaagtac aaattatggt gactggtggg gtaaatctca
    19321 caaatgccaa agactggttt ctatctggag taacagccat tggtattggt ggagagttca
    19381 ataaactagc agctttgggg gaatttgata aaatcacaga aatggctaaa caatacaaaa
    19441 tacagctaaa tctgaattaa cacaataaaa agacaaggca cccccacacc tcaatcatga
    19501 gacagaagaa aagaactttt ataaagccat ttttctgtag taccgtttat cttaatcaga
    19561 ctcattatct cgattttctt atcagaccga gataattttt agctaatctt cctgtagaca
    19621 taaccgttct acttccgaca actgatccat gtcttcacca aagatatact acttgcccgc
    19681 agattgatat aagtagtgaa gctcatcatt ctcatatcat ttcagcaaat aatagatttg
    19741 atatcatttt tatatccaaa aacttagcaa tgacctatca ggcttacccg cttttcctat
    19801 ctcaacacag gctaacttag ctcctatcaa atacattttt taaacataat taaaaacatt
    19861 tctgattata gtttattaga tttcaacaga agtatttttc ctgcgtctca gcttttgggt
    19921 gtaccgttat tttgaagttt tgatagttga gtgatatcat taatagtcat tttttagaca
    19981 ttctaaacct cctttctaat ttgaccgatt ttatttttta taacgactcg ttttactgt
//
LOCUS       DATRUK010000034        19462 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.034, whole genome shotgun sequence.
ACCESSION   DATRUK010000034 DATRUK010000000
VERSION     DATRUK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19462
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.034"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..317
                     /locus_tag="VBE34_001203"
     CDS             <1..317
                     /locus_tag="VBE34_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7263925.1"
                     /translation="LTIEFLADYMGYSKTHFMTVFKQHTGTSCTEFIIQVRLNKASEH
                     LINSTTAIIDIANSVGFNNLSNFNRQFKRYYHTTPRQYRKQFTNKKDGSTLVYRNINT
                     KK"
     gene            complement(374..1906)
                     /locus_tag="VBE34_001204"
     CDS             complement(374..1906)
                     /locus_tag="VBE34_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEO7263926.1"
                     /translation="MENHKNEFQFSLESILGFVWRGIVVGLIAGFVVSIFRLAIEKIF
                     LVVMELYKSAHYQPIILLSITVTSIIAAVIIGFFIKSDPDIKGSGIPHVEGELKGMLS
                     PDWFSIVWKKFIAGILAISSGLMLGREGPSIQLGAMTGKGIAQYLNASRMEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLVWITALVASLVANFVSLNIFGLTPVLA
                     LPSELPSLNLNFYWIFLLMGLFLGILGFIYEWVILRFHVIYDYLGKLFHLPSHLYGIL
                     AVIFILPIGYYFPQLLGGGNGLIVSLPRSNLSLMMLGLFFLIRFLWSMLSYSSGLPGG
                     IFLPILALGSLAGAFFAVGMQYFGIISHQQISLFVVLGMAGYFGAISKAPLTAMILVT
                     EMVGDLKQLMAIGIVTMVSYIVMDLLKGEPIYEAMLAKMTFNPKDKVMTPTLIELTVS
                     DKISGKYVRDLELPENVLITTQIHHKTSAVVSGNTILNAGDTIFLVVNESEIKEVREQ
                     LM"
     gene            complement(2015..3088)
                     /locus_tag="VBE34_001205"
     CDS             complement(2015..3088)
                     /locus_tag="VBE34_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7263927.1"
                     /translation="MRRVYSFLGGIILVILILFGLTTYLEKKSSSTPNSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMHTKIKQGGTTYDIAVPSDYMIDKMIKEN
                     LLVKLDHSKIANWDAIGARFKNLSFDPKNKYSIPYFWGTVGIVYNDQLVKTPPKHWDD
                     LWRPEFRNKIMLVDSAREVIGVGLNSLGYGLNTKNISELKAASKKLDALTPNVKAIVA
                     DEMKGYMIQGDAAIGVTFSGEAREMLDGNKHLHYVVPSEGSNLWFDNIVIPKTVKHRK
                     EAYAFINFMMEPKNAAQNAEYIGYATPNLKAKALLPADIKNDKAFYPPDKTIDHLEVY
                     NNLGQKWLGIYNDLYLQFKMYRK"
     gene            complement(3081..3857)
                     /locus_tag="VBE34_001206"
     CDS             complement(3081..3857)
                     /locus_tag="VBE34_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7263928.1"
                     /translation="MKKFANIYLAFVFIILYIPIIYLIFYSFNKGGDMNSFTGFTFSH
                     YGELFQDSRLMLILVQTFFLAFLSALLATIIGTFGAIWIYQVRRRHQTSILSLNNILL
                     VAPDVMIGASFLLVFTVIGLQLGFTSVLLSHVAFSIPIVVLMVLPRLKEMNDDMINAS
                     YDLGASTWQMLKEVMLPYLSSGIISGFFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARRGISLEINALSTIVFLFSILLVIGYYYISKEKGEKNA"
     gene            complement(3854..4648)
                     /locus_tag="VBE34_001207"
     CDS             complement(3854..4648)
                     /locus_tag="VBE34_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7263929.1"
                     /translation="MKKTSSLFSIPYMAWLFLFVLAPVALIAWNSFFDINGHFTLANY
                     QTFFSSGTYLKMSFNSVLYAGIVSFITLLISYPAAYLLTKLKHKQLWLMLIILPTWIN
                     LLLKAYAFMGIFGQQGGINAFLTFIGIGPKQILFTDFSFLFVAAYIELPFMLLPIFNA
                     LDDIDQNLIYASDDLGANAWQTFQKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLITQNKGMGSTIGVILILVMVAIMWLTKERKA"
     gene            complement(4632..5786)
                     /locus_tag="VBE34_001208"
     CDS             complement(4632..5786)
                     /locus_tag="VBE34_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7263930.1"
                     /translation="MTNPIIAFKNVSKVFEDSNTVVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLEASTGDIYLDGKRINDVPTNKRDVHTVFQNYALFPHMTVFENVAF
                     PLKLKKMDKKEIQKRVQETLKMVRLEGFEKRAIQKLSGGQRQRVAIARAIINQPKVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILSGKMIEDYLVEFNGKRFEAVDGGMRPNESVQV
                     VIRPEDLQITLPDEGKLQVKVDTQLFRGVHYEIIAYDDLGNEWMIHSTRKAIEGEVIG
                     LDFTPEDIHIMRLNETEEEFDARIEEYVDTDDHEDGLINAIEEERNEENL"
     gene            complement(5835..6737)
                     /gene="murB"
                     /locus_tag="VBE34_001209"
     CDS             complement(5835..6737)
                     /gene="murB"
                     /locus_tag="VBE34_001209"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEO7263931.1"
                     /translation="MIKTIQKELEGLDIRFDEPLKKYTYTKVGGPADYLAFPRNRLEL
                     SRIVKFANSQNIPWMVLGNASNIIVRDGGIRGFVIMFDKLSTVTVNGYVIEAEAGANL
                     IETTRIARYHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILLSAQVLTPQGELKT
                     IEARNMQFGYRHSVIQESGDIVISAKFALKPGDHLMITQEMDRLTYLRELKQPLEYPS
                     CGSVFKRPPGHFAGQLISEAHLKGQRIGGVEVSQKHAGFMVNIAEGSAQDYENLIEHV
                     INTVESTSGVHLEPEVRIIGESLL"
     gene            complement(6881..7369)
                     /gene="folK"
                     /locus_tag="VBE34_001210"
     CDS             complement(6881..7369)
                     /gene="folK"
                     /locus_tag="VBE34_001210"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEO7263932.1"
                     /translation="MTTVYLSLGSNISDRETFLKQALFSIDHLQKTKVAQISAIYETA
                     AWGNTNQEDFFNICCQVETDLAPFELLDYCQEIEKRLKRVRHEHWGPRTIDIDILLFG
                     NQVINQEDLVVPHPYMTKRAFVLVPLLEIAPQLSLPNGSKLEDYLEKLNLGEVHYLKP
                     VE"
     gene            complement(7366..7728)
                     /gene="folB"
                     /locus_tag="VBE34_001211"
     CDS             complement(7366..7728)
                     /gene="folB"
                     /locus_tag="VBE34_001211"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEO7263933.1"
                     /translation="MDKIYLNKCRFYGYHGAFSEEQTLGQVFQVDAVLSLDLAKASQT
                     DDLIDTVHYGEVFDCIKNHVENEQYQLIEKLAGVIVEDIFLQFHPVQAITLKITKDNP
                     PINGHYESVGIELERKRV"
     gene            complement(7730..8533)
                     /gene="folP"
                     /locus_tag="VBE34_001212"
     CDS             complement(7730..8533)
                     /gene="folP"
                     /locus_tag="VBE34_001212"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEO7263934.1"
                     /translation="MKIGQYDITGKACIMGILNVTPDSFSDGGSYTTIDSALNQVGEM
                     LEQGVAIVDIGGESTRPGAVFVTAEEEIKRVVPMIKAIREVYPDLLLSIDTYKTEVAQ
                     AALDAGVHILNDVWSGLYDGKMLSLAAERNVPIILMHNQEEAVYQDIKKEVCEFLLER
                     AERALEAGVSKDNIWIDPGFGFAKTEEQNLELLKGLEQVCDLGYPVLFGISRKRTVNY
                     LLGGNREVTERDMGTAALSAWAIAKGCQIVRVHNVEVNKDIVTVISQLV"
     gene            complement(8537..9100)
                     /gene="folE"
                     /locus_tag="VBE34_001213"
     CDS             complement(8537..9100)
                     /gene="folE"
                     /locus_tag="VBE34_001213"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEO7263935.1"
                     /translation="MPNQEKMEKAIYQFLEALGENPDREGLKDTPKRVAKMYIEMFSG
                     LNQDPKEQFTAVFSENHEEVVIVKDIPFYSMCEHHLVPFYGKAHIAYLPNDGRVTGLS
                     KLARAVEVASKRPQLQERLTAQVAQALEDALAPKGIFVMIEAEHMCMTMRGIKKPGSK
                     TITTVARGLYKDDRYERQEILSLIQKG"
     gene            complement(9119..10381)
                     /locus_tag="VBE34_001214"
     CDS             complement(9119..10381)
                     /locus_tag="VBE34_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEO7263936.1"
                     /translation="MTYQEALEWIHSKLAFGIKPGLERMRWMLEQLGNPQNNLSAIHV
                     VGTNGKGSTTSYLQHIFTNSGYQVGTFTSPYIVDFRERISIDGQMIPESDFIKLVETV
                     RPVVERLHLETNLEPATEFEVITVLMFYYFGNSCPVDIVIIEAGMGGYYDSTNMFKAL
                     AVTCPSIGLDHQEVLGRTYVDIAEQKVGVLKKGVPFVYANDRQDVEEVFQIKAKETHS
                     QTYRLHNDFYIKEEENYFNYIGPQANIDHIQLQIPGHHQVSNASIAITTSLLLRDKYP
                     KLTLQTIKDGLEMTKWVGRTELIFPNVMIDGAHNNESVDALVQVIKKYQQKNVHILFA
                     AINTKPIESMLESLSSIAPVSVTSFDYPKSINLDKYPKAYTRVSDWKKWLHDINLTSD
                     KDFYVITGSLYFISQVRQELLLIKTTTT"
     gene            complement(10383..11270)
                     /gene="rarD"
                     /locus_tag="VBE34_001215"
     CDS             complement(10383..11270)
                     /gene="rarD"
                     /locus_tag="VBE34_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEO7263937.1"
                     /translation="MRKDNLGIILGLSAYVLWGLLSLYWKLLSGIEAYSTFAYRIIFT
                     VLTMLIYMLVSGRKTVYLKDLKGLVNNKKSFWTMFVASILISINWLVYIFAVTHGHAT
                     EASLGYYMMPIISILLSVLVLREHLARVVSLAILIAIMGVGILVYQTGHFPLISLTLA
                     LSFGFYGLLKKSISLSSDFSMLVESSFIAPFALIYIVFFAKDFLTDYNILQLVLLSLS
                     GIITAVPLLLFAEAIKRAPLNIIGFIQYINPTIQLLLALFIFKETIVSGEVIGFIFIW
                     LAILVFSIGQVHTMLKKGK"
     gene            complement(11257..12123)
                     /locus_tag="VBE34_001216"
     CDS             complement(11257..12123)
                     /locus_tag="VBE34_001216"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEO7263938.1"
                     /translation="MRIIVPATSANIGPGFDSIGVALSKYLSIEVLEESTEWLVEHNL
                     VNIPKDHTNLLIQTALHVKSDLAPHRLKMFSDIPLARGLGSSSSVIVAGIELANQLGN
                     LALSQKEKLEIATRLEGHPDNVAPAIFGDLVISSIVKNDIKSLEVMFPDSSFIAFIPN
                     YELKTSDSRNVLPQKLSYEDAVASSSVANVMVASLLKGDLVTAGWAIERDLFHERYRQ
                     PLVKEFGVIKQISTQNGAYATYLSGAGPTVMVLCSKEKEQPIVTELSKLCLDGQIQVL
                     NIERKGVRVEKR"
     gene            complement(12125..13408)
                     /locus_tag="VBE34_001217"
     CDS             complement(12125..13408)
                     /locus_tag="VBE34_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEO7263939.1"
                     /translation="MTIKIALLGFGTVAKGIPYLLKENQHKLLSLEGEDIVIDKVLVR
                     DNESRQRFINQGFTYNFVTEINTILQDPQIDIVVELMGGIEPAKTYLSQALGFGKHIV
                     TANKDLIALHGKELMDLADARGLALFYEGAVAGGIPILRTLSHSFASDKMTRLLGILN
                     GTSNFMLTKMFEEGWSYEQALKKAQELGYAESDPTNDVEGIDTAYKATILSQFGFGMP
                     IDFDDVNYKGISSIRSEDVEVAQEMGFAIKLVTDLRETPTGISVDVSPTLISQEHPLA
                     AVNHVMNAVFIESIGIGQSLFYGPGAGQNPTATSVLADIIDISRSIRSQIKIKPMNTY
                     HCPCRLSMQSDIFNEYYLAISLRNAEDSDTLGRYFEQENIGLKNVIEKALDDKQQEIY
                     VLTDEVSQEKITQFIEEFPESGVIQLINVFKVIGG"
     gene            13585..14473
                     /locus_tag="VBE34_001218"
                     /pseudo
     CDS             13585..14473
                     /locus_tag="VBE34_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645955.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(14496..16043)
                     /locus_tag="VBE34_001219"
                     /pseudo
     CDS             complement(14496..16043)
                     /locus_tag="VBE34_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
     gene            complement(16186..17562)
                     /locus_tag="VBE34_001220"
     CDS             complement(16186..17562)
                     /locus_tag="VBE34_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEO7263940.1"
                     /translation="MAYKTIYPYTNEVLHEFDNISDSDLEQSLDIAHALYKTWRKEDN
                     VEERQNQLHKVADLLRKDRDKYAEVMTKDMGKLFTEAQGEVDLCADIADYYADNGQKF
                     LKPVPLESPNGEAYYLKQAVGVLLAVEPWNFPFYQIMRVFAPNFIVGNTMLLKHASIC
                     PASAQAFEDLVREAGAPEGAFKNIFASYDQVSNLISDPRVAGVCLTGSERGGASIAAE
                     AGKNLKKSSMELGGNDAFLILDDADFDLLSKTIFFARLYNAGQVCTSSKRFIVMADKY
                     DEFVNMVVETFKSAKWGDPMDSETTLAPLSSAGAKDDVLKQIKLAVDHGAEVVFGNDT
                     IDHPGNFVMPTVLTNITKANPIYNQEIFGPVASIYKVDTEEEAIALANDSSYGLGSTV
                     FSSDPEHAKKVAAQIETGMTFINSGWTSLPELPFGGIKNSGYGRELSQLGFDTFVNEH
                     LVFTPNSD"
     gene            complement(17636..18643)
                     /locus_tag="VBE34_001221"
     CDS             complement(17636..18643)
                     /locus_tag="VBE34_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEO7263941.1"
                     /translation="MLFKEKIPGLILCFIIAIPSWLLGLYLPLIGAPVFAILIGIIVG
                     SFYQNRQLFNKGIAFTSKYILQTAVVLLGFGLNLMQVMKVGISSLPIIIMTISISLII
                     AYVLQKLFKLDKTIATLIGVGSSICGGSAIAATAPVINAKDDEVAQAISVIFLFNILA
                     ALIFPTLGNFIGLSDHGFALFAGTAVNDTSSVTATATAWDAINHSNTLGGATIVKLTR
                     TLAIIPITIVLSIYHMKQTQKEQSVSVTKIFPKFVLYFILASLLTTIVASLGFSLRIF
                     EPLKVLSKFFIVMAMGAIGINTNVSKLIKTGGKSILLGAACWLGIIIVSLTMQAILGT
                     W"
     gene            complement(18751..19437)
                     /locus_tag="VBE34_001222"
     CDS             complement(18751..19437)
                     /locus_tag="VBE34_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263942.1"
                     /translation="MKKFLKVFLTVLVLILIVGAGGLYFWNNRQSLEGKWRTVSLEKQ
                     VEKEIEQQLGSQAADMGISAADLVKGANMHMNVKNDEAKITVTAQIDEVKFHQAIKTF
                     IDKALEKQLKDQGLTYNDLSEAGKKIFDETKITDKQIDQQIDRSFQSAAQAVGGKYNT
                     NTGEMTLPVMDGKVHRLTSVIKVSHINKKANAFYGNIVKNGEKTAYKKEGSKLILGNE
                     KSYPFMKVTK"
BASE COUNT         6591 a         3929 c         2920 g         6022 t
ORIGIN      
        1 atcttaccat tgaattttta gctgattata tggggtatag taagacacat tttatgaccg
       61 tatttaaaca gcatactgga acatcctgta cagaatttat catacaagtt cgtcttaata
      121 aagctagtga gcatcttatt aactccacta cggccattat tgatattgcc aacagcgttg
      181 gttttaataa tctatcaaat tttaatcgcc aatttaaacg ctattatcat acaactcctc
      241 gccagtaccg caaacaattt accaacaaaa aagacggtag tacgcttgtc tatagaaata
      301 taaatacaaa aaaatagaaa ctagttaaat ccatgactag tttctatttt ttattattta
      361 agcgttaatt agtttacatc agctgctcac gtacttcttt aatttccgat tcattcacaa
      421 ctaaaaaaat ggtgtcgcca gcattcaaga tagtatttcc tgatactaca gccgatgtct
      481 tatgatgaat ttgagtcgtt attagcacat tttctggtaa ttctaaatct cggacatact
      541 ttcctgatat tttatctgaa acagttaatt caatcaatgt aggtgtcatt actttatctt
      601 ttggattgaa ggtcatttta gccaacatcg cttcataaat aggttctccc tttaataaat
      661 ccatgacaat ataagatacc attgttacaa ttccaatagc cattaattgt tttaaatcgc
      721 caaccatttc tgtaaccaaa atcatagctg ttagtggtgc ttttgagatt gcaccaaaat
      781 agcccgccat tcctagaaca acaaataaag atatctgctg atgagatatt ataccaaagt
      841 actgcatacc tactgcaaaa aaagcacctg ctaaagatcc taaagctaga attggtaaaa
      901 aaattcctcc tggcaagcca ctactgtaac tcaacatact ccataaaaaa cgaatgagga
      961 aaaataatcc taacatcatc agactaagat tactcctagg cagtgataca attaagccat
     1021 ttccaccccc caataattgt gggaaatagt aaccaatagg aagaataaaa attaccgcta
     1081 atataccata taggtgagat ggtagatgaa atagtttacc taaataatca tagatcacat
     1141 gaaatctcaa aattacccac tcatagataa agcctaagat acccaaaaag agacccatga
     1201 gtaaaaaaat ccaataaaag ttgagattta aagatggtaa ttctgatggt aaagctaaaa
     1261 ctggggttaa accaaagata ttaagtgaga caaagtttgc cactaaactt gctaccaaag
     1321 ctgttatcca aactaagcga gaaaaatgat ggtaaatttc ttcaactaca aataaaaggc
     1381 ctgcaatagg agcattgaag gctgctgaaa gacctgctgc tgcaccacta gcaattaaaa
     1441 cacgtttttc cattcgacta gcattgagat actgtgcaat tcccttacct gtcatagctc
     1501 ctaactgaat actgggacct tcgcgaccaa gcataagtcc cgaagaaata gctaaaatac
     1561 ccgcaatgaa tttcttccat acaatactaa accagtctgg cgatagcatt cctttaagtt
     1621 caccttcaac atgcgggata cctgaacctt taatatcagg atcagatttg ataaaaaaac
     1681 ctattatcac tgcagcgata atactagtaa ccgtaatgct caataaaata attggttgat
     1741 aatgggcact tttataaagt tccataacaa ctaaaaaaat tttttcaatc gctaatcgaa
     1801 atatgctgac aacaaatcct gctattaagc ctactactat tcctctccaa acaaatccca
     1861 gaattgattc aagtgaaaac tgaaattcat ttttatgatt ttccatattt tcctcctttt
     1921 cttacaatta ttttaacaaa aatatcacaa actttctagg aactcttggt aactaaaaat
     1981 acctctcatc agagaagtac ttttagttaa tagattactt acgatacatt ttaaattgta
     2041 agtacaaatc attataaata ccaagccatt tttgacccaa gttattataa acttctaaat
     2101 gatcaattgt tttatcagga ggataaaatg ctttatcatt tttgatatct gctggtaaca
     2161 gcgcttttgc tttgagatta ggggtcgcat aaccaatata ttctgcattt tgtgccgcat
     2221 ttttaggttc catcatgaaa ttaataaagg cataagcttc tttgcgatgc tttactgttt
     2281 tggggattac aatattatca aaccaaaggt tcgatccttc actaggaaca acgtaatgca
     2341 aatgcttgtt cccatcaagc atttctcgtg cttctccaga gaatgtaaca ccaatcgctg
     2401 catctccctg aatcatatag cctttcatct catcagcaac aatagcctta acgtttggtg
     2461 ttagagcatc taacttttta gaagcggctt ttaattctga tatgttcttt gtattcaatc
     2521 catatcccaa ggaatttaaa ccaactccaa taacctcacg tgcactatca actagcatga
     2581 ttttatttcg aaactctgga cgccataaat catcccaatg tttaggagga gttttaacta
     2641 attgatcatt ataaacaatc ccaacagttc cccaaaaata aggaatcgaa tatttatttt
     2701 taggatcgaa actcaaattt ttaaacctag ctccaatagc atcccaatta gctatctttg
     2761 aatgatcaag tttaaccaga aggttttctt ttatcatttt gtcaatcata taatcactag
     2821 gtacagcaat atcatatgtc gtcccaccct gcttaatttt ggtgtgcata gcctcattag
     2881 agtcaaacgt ttcgtattgt acttcaattc cagtttcttt tgtaaatttt tttaataagg
     2941 ctggatcaat atagtctccc cagttataaa taactaactt atctgagtta ggtgttgaac
     3001 tactcttctt ttctaagtaa gtggttaaac caaagaggat caggataaca agaataatac
     3061 cgcctaaaaa ggaataaact ctacgcattc ttttctccct tctctttcga aatatagtaa
     3121 taaccaatta ccaataaaat actaaataaa aagacaatag ttgacaaggc attaatttcc
     3181 aatgatatac cgcgacgtgc gcgtgaatag atttctactg aaagcgttga aaagccatta
     3241 cctgtaacaa agaaagtaac cgcaaaatca tctagcgagt aagtgaaagc cataaagaaa
     3301 cctgaaataa tacccgacga caaatagggt aacataactt ctttaagcat ttgccaagtt
     3361 gaagctccta aatcatagga agcattaatc atatcatcat tcatttcttt taaacgaggt
     3421 aagaccatta aaacaacaat aggaatagag aatgcaacat ggctcaataa tacagaagtg
     3481 aaacctaact gtaaaccaat aacagtaaat actaagagaa aacttgcacc aatcataaca
     3541 tctggtgcta ctaaaaggat attattaagc gataaaatag aggtttgatg tctacgtctt
     3601 acctgataaa tccaaatagc tccaaaggta ccaataattg tagctaataa ggcgctaaga
     3661 aaagccaaaa agaatgtttg cactaaaatt agcattaagc ggctatcctg aaacagttct
     3721 ccatagtgac taaaggtgaa tccagtaaaa gaattcatat ccccaccttt attgaaagaa
     3781 taaaaaatca aataaataat aggaatataa aggataataa aaacaaatgc taaatagata
     3841 tttgcgaatt ttttcatgct ttcctctcct ttgttaacca cataatagca accatcacta
     3901 aaattaaaat aacccctatg gttgagccca tacctttatt ttgtgtgatt aaaaaatgtt
     3961 gttctatggc tgtccctaaa gtaataactc gattaccacc aataagacgc gtaagcataa
     4021 agagacttaa actaggaata aagacagatt gtacacctgc tcttacacca ttcagagata
     4081 gaggaaatat aaccttctga aaagtttgcc atgcatttgc acccaagtca tcacttgcat
     4141 aaatgagatt ttgatcaata tcatccaagg cattaaaaat gggtaataac ataaacggta
     4201 attcaatgta agctgctaca aaaagaaatg aaaaatctgt aaatagaatc tgttttgggc
     4261 cgatacctat aaaggttaag aaggcgttaa ttcccccttg ttgaccaaat atccccataa
     4321 aagcataggc ttttaacaaa agattaatcc atgttggcaa aataattagc attagccata
     4381 attgcttgtg ttttaatttt gtaagcaaat aagcagctgg atagctaatc aaaagcgtta
     4441 tgaaactaac gatgcctgca tataggaccg agttaaagct cattttaaga taggttcctg
     4501 agctaaaaaa cgtctggtaa ttagctaaag taaagtgccc attaatatca aagaacgagt
     4561 tccaagcaat caaagcaaca ggtgctaata caaataagaa taaccaagcc atataaggaa
     4621 ttgaaaatag actagaggtt ttcttcattt ctctcctcct cgatagcatt gattaaacca
     4681 tcttcatgat cgtcagtgtc tacatattct tcaatgcgcg catcaaattc ttcttctgtt
     4741 tcattaagac gcataatatg tatatcttct ggagtgaaat ctaacccaat aacttcaccc
     4801 tcaatagctt tacgagttga atgaatcatc cattcattac ctaaatcatc ataagcaata
     4861 atttcatagt ggacacctcg aaaaagttgt gtgtccactt taacttgaag ttttccttca
     4921 tcgggtaggg taatttgaag atcttctgga cgaataacta cttggactga ttcattaggg
     4981 cgcataccgc catcaacagc ctcaaagcgc ttaccgttaa attcaaccaa ataatcctca
     5041 atcatcttgc cagataagat atttgattca cctataaaag ttgctacaaa atgattgata
     5101 ggttcatcgt agatatctac tggtgttcct gattgaacaa tttcaccttc attcatgacg
     5161 aaaatccaat cactcattgc caacgcttct tcttgatcat gtgtgacaaa aacaaaggta
     5221 attcctaaac gttgttgcag ttcgcgcaat tcatattgca tctctgtacg caatttcaaa
     5281 tccaatgctg ataagggttc atctagtaag actactttag gttgattgat gatagcacgc
     5341 gctatcgcaa cacgctggcg ttgtcctcct gataattttt gaatagctcg cttttcaaac
     5401 ccttctaaac gaaccatttt tagagtttct tgaacacgtt tttgaatttc tttcttatcc
     5461 atttttttca actttaatgg aaaagcaacg ttttcaaaaa ccgtcatatg tggaaacaaa
     5521 gcatagtttt ggaaaacggt atgaacatcc cgtttattag tgggaacatc attgatgcgt
     5581 ttcccatcta agtaaatatc gcctgtactc gcctctagca aacctgcaat aatatttaag
     5641 atagttgatt taccacttcc tgaggcccct aataaggtgt aaaatttacc ttcttcaagt
     5701 tcaaaattaa tatcttttaa gacaaccgtg ttgctgtctt cgaatacctt tgatacattt
     5761 ttaaatgcaa tgattgggtt agtcaattct cataaatcct ccatttttct gactaaagta
     5821 agtactaaac tactttataa tagcgactca ccgataatac gaacttctgg ttccaaatgg
     5881 acacctgaag ttgattcaac ggtgttaata acatgttcaa taagattttc ataatcttga
     5941 gctgaacctt ctgcaatgtt aaccataaaa ccagcatgtt tttgactaac ctctactcca
     6001 ccaatacgtt gacctttaag atgtgcttcg ctaatgagtt gccctgcaaa atgtcctgga
     6061 ggacgtttga acacagaacc acaagaaggg tattctaaag gttgtttcag ctcacggaga
     6121 taggttaaac gatccatttc ctgagtaatc atgagatgat ctcctggttt taaggcaaac
     6181 ttagctgata taacaatatc tccactctct tgaatgacag aatgacgata accaaattgc
     6241 atattcctag cttcaatcgt tttgagttca ccctgtggtg tcaaaacttg tgcagacaat
     6301 aaaatatgag caatttcacc accgtaagcg ccagcattca taaagactgc tccacctaca
     6361 cttccaggaa ttccgcaagc aaactcaaag ccagttaaac tatgataacg tgcaatacga
     6421 gttgtttcaa tgagattagc tcctgcttct gcttcaataa cataaccgtt gactgtaaca
     6481 gttgacagtt tatcaaacat gataacaaaa ccacggatac caccatctct aacgataata
     6541 ttactcgcat ttcctaaaac catccaagga atattttgac tgttagcaaa cttaacaata
     6601 cgcgatagtt caaggcgatt ccttgggaat gcaagataat cagcaggtcc tcctacttta
     6661 gtataagtat atttctttaa aggctcatca aagcgaatat cgagaccttc taattctttt
     6721 tgtattgttt taatcataac ctttatctca attctgtttt attgtgtttt atagacacaa
     6781 aaaatcggca taaaaccgat ttctattata ccaaaaatat aggttgttga cgatacatta
     6841 tcacaatttc tgtgtatttt aattttaaaa tcctaactga ctattcgact ggttttaaat
     6901 aatgaacttc tcctaaattc aatttttcga gatagtcttc tagcttagaa ccattaggta
     6961 aacttagttg aggtgctatt tcgagtaacg gaactagaac aaatgctcgc ttcgtcatat
     7021 acggatgggg tactactaaa tcttcttggt taatcacttg attaccaaaa agtaagatat
     7081 caatatctat tgtccttgga ccccagtgct catgtctcac gcgctttaag cgtttttcaa
     7141 tttcttgaca ataatccaat aattcaaaag gtgctaagtc tgtctctacc tgacaacata
     7201 tattaaaaaa atcttcttgg ttagtattcc cccaagcagc tgtttcataa atagcggaaa
     7261 tttgcgcgac ttttgtcttt tgcaaatgat caattgaaaa gagagcctgc ttcagaaaag
     7321 tttctctatc actaatatta ctacctaaac tcaagtaaac ggttgtcata ctcgcttgcg
     7381 ctccaattca attccaactg attcatagtg cccattaatt ggtggattgt cttttgtaat
     7441 ctttaatgta atagcttgta ctggatgaaa ctgtaagaaa atatcttcaa caataacacc
     7501 tgctaatttc tctattaatt gatactgttc gttttcaaca tgatttttaa tacagtcaaa
     7561 aacttcacca taatgaaccg tatcaattaa atcatcagtt tgtgatgctt ttgctaaatc
     7621 aagtgataga acagcatcaa cttgaaaaac ttgccccaat gtttgttctt ccgaaaaagc
     7681 accatgataa ccataaaacc gacatttatt taaataaatc ttatccatat tacaccaact
     7741 gactaattac tgtcacaatg tccttattta cttcaacatt gtgaacacgt acaatctgac
     7801 acccctttgc tattgcccat gccgataaag cagcagtccc catatcacgt tcagttactt
     7861 ccctattacc acctagaaga taattaacag tacgttttcg agagatccca aataacacag
     7921 gataacctaa atcacacact tgttccaaac cttttaatag ctctaaattt tgctcctctg
     7981 ttttagcaaa accaaagcca ggatctatcc aaatattatc ctttgaaaca cctgcttcta
     8041 aagcacgctc tgctctttca agtaagaatt cacaaacttc ttttttgata tcttgataaa
     8101 cagcttcttc ctggttatgc atcaaaataa ttggaacatt tcgttctgct gctaaagata
     8161 gcattttacc atcatataaa cctgaccaaa cgtcatttaa aatatgtaca cctgcatcaa
     8221 gggctgcttg agctacctct gttttataag tatctattga taacaaaaga tcagggtaga
     8281 cctctctaat agctttaatc attggaacta cacgtttaat ttcttcttca gcagtcacaa
     8341 atactgcacc tggccgcgtt gattctcccc caatatctac aattgccacc ccttgttcaa
     8401 gcatctcacc aacttgattt aaagctgaat caattgtggt ataagaacca ccatctgaga
     8461 aggaatcagg cgttacattt aaaatcccca tgatacaagc tttacctgta atatcatatt
     8521 gtcctatttt catcttttat cctttttgta ttagcgatag aatttcttgt ctttcatatc
     8581 gatcgtcctt gtataaacca cgagctaccg ttgtaatcgt cttacttcct ggttttttaa
     8641 tgccacgcat tgtcatgcac atatgctctg cttctatcat aacaaaaata cctttaggcg
     8701 ctaaagcatc ctctaaagct tgtgcaactt gtgctgttaa acgttcctga agttgaggac
     8761 gcttactcgc cacttcaact gcacgtgcaa gcttactaag tccagtaaca cgtccatcgt
     8821 ttggtaaata agcaatatga gctttaccat aaaaaggaac caaatgatgt tcacacatgg
     8881 aatagaatgg aatatccttg acaatcacaa cctcttcatg attttcagaa aagactgcag
     8941 tgaattgctc ctttggatct tgattaagtc ctgagaacat ttctatatac atttttgcca
     9001 cacgcttcgg tgtatctttc aacccttctc gatcaggatt ttctcctaaa gcctctaaaa
     9061 attgataaat agctttttcc attttttctt gattgggcat taatttatcc tatccttttt
     9121 aagttgttgt agttttgatt aatagtagtt cctgacgaac ttgagaaata aaataaaggg
     9181 agccagtaat cacatagaaa tctttatctg atgtcagatt tatatcatgt agccatttct
     9241 tccaatcact aactctagtg tacgctttag gatatttgtc taagtttatt gatttagggt
     9301 aatcaaagct agtaaccgaa accggagcga tactagacaa tgattctaac atactctcta
     9361 tcggtttagt attaattgca gcaaatagaa tatgcacgtt tttctgctga tattttttta
     9421 taacttgaac gagagcatcg acactttcat tattatgagc gccatcaatc attacatttg
     9481 gaaagattaa ttctgtacgg cctacccatt ttgtcatttc aagcccatct ttaatggtct
     9541 gtagggttaa ctttggatat ttgtctctta ataataaact ggtagttata gctatgctag
     9601 cattggaaac ttggtgatgt cccggaattt gcaactgaat atgatcaata tttgcttgag
     9661 gacctatata gttaaaataa ttttcctctt cttttatata aaagtcattg tgtaaacgat
     9721 aagtctggct gtgggtttcc ttggctttta tttgaaagac ttcttcaaca tcctgacgat
     9781 cattagcata tacgaatgga actccttttt ttaaaacacc tactttttgt tcagctatat
     9841 ctacataagt tcgtcctaaa acctcctgat gatctaatcc aattgacgga caagtaaccg
     9901 ctaaagcctt gaacatgttg gtagaatcat aataaccgcc cattccagcc tcaataatga
     9961 cgatatcaac tggacagcta ttgccaaaat aatagaacat caaaactgtt ataacttcaa
    10021 attctgtagc tggttctagg ttggtctcaa ggtgcaaccg ttcaacaact ggacgaacag
    10081 tctcaacaag tttgataaaa tcagactctg gaatcatttg accatcaatg ctaatacgct
    10141 ctctaaagtc aacaatatat ggtgatgtaa atgttccaac ttgataacca gaatttgtaa
    10201 aaatatgttg taaataacta gttgttgatc ctttaccatt cgttccaaca acatgaatgg
    10261 cagataggtt gttttgaggg tttcctaact gctctagcat ccatctcata cgttccaaac
    10321 ctggtttaat accaaaagct aatttgctat gaatccattc tagcgcttct tgataagtca
    10381 tcttatttac ctttctttaa catcgtatgt acttgtccaa tactaaaaac taaaatagca
    10441 agccagataa aaataaaacc tattacctcc ccactaacga tagtttcttt aaaaataaac
    10501 aatgctagca ataattggat ggttggattg atgtattgga taaaaccgat aatatttaga
    10561 ggagccctct taatggcttc tgcaaataat aaaagaggaa ctgctgtaat gattcctgaa
    10621 agtgagagta aaactaattg taatatatta taatcagtta aaaaatcttt ggcgaaaaac
    10681 actatataaa tgagtgcaaa cggagcaata aaactacttt ctaccagcat cgaaaaatcg
    10741 ctagataaag aaattgattt ttttaataac ccataaaaac caaaagaaag cgctagagtt
    10801 agtgaaatta aaggaaaatg accagtttga taaactaaaa ttccaactcc catgattgca
    10861 atcaaaattg caaggctaac tactcttgcc aggtgttcac gtaaaacaag gaccgacaac
    10921 aaaattgaaa taatcggcat catataataa ccaagacttg cctcagtagc gtgaccatgc
    10981 gtgactgcaa aaatatagac caaccaatta attgaaatta aaatactggc tacaaacatt
    11041 gtccaaaatg actttttatt attaactaag ccttttaagt cttttaaata gactgttttt
    11101 ctacccgaaa cgagcatata aataagcata gttaaaacag tgaatataat cctataagca
    11161 aacgtactat atgcttcaat tccagataat aatttccaat aaagagataa aagcccccat
    11221 aaaacatagg ctgacaagcc aaggataatt cctaaattat cttttctcaa ctctcactcc
    11281 ttttcgctca atattaagta cttgaatctg tccatctaaa cataatttac ttaactctgt
    11341 gacaattggt tgttcttttt ctttcgaaca cagaaccatt accgtcggtc ctgctccaga
    11401 caaataagta gcatacgcac cgttttgagt gctgatttgt tttataactc caaattcctt
    11461 caccaaaggt tgacgatatc tttcatgaaa gaggtccctt tctatagccc atccagcagt
    11521 taccaaatca cctttcaaca acgacgcaac catcacatta gcgacgctgc ttgaagcaac
    11581 tgcatcctca taactcaatt tttgaggtag tacatttcga ctatcacttg tttttaattc
    11641 ataatttgga ataaaagcga taaaggaact atctggaaac atcacttcca aacttttaat
    11701 atcatttttc actattgatg aaataactaa atcaccaaaa atggccggtg ctacattatc
    11761 aggatgtcct tctaatcggg tagctatctc taatttttct ttttgagaaa gagccaaatt
    11821 tcctaattga ttggctaatt ctattcctgc aacaattaca gaggaagaag aacctaaacc
    11881 tctggccaaa ggaatatctg aaaacatttt taagcggtgt ggtgccaaat ccgacttaac
    11941 atgcaaagct gtttgaataa ggaggttagt atgatctttt ggaatattta ctaaattgtg
    12001 ttcaactaac cactctgtac tttcttctaa tacttcaatg cttaaatact ttgataacgc
    12061 aactcctatt gaatcaaagc ctggaccaat attcgcacta gttgcaggta caattatcct
    12121 catttcaccc tcctattact ttgaaaacat tgattaactg aatgacacca ctctcaggaa
    12181 actcctcaat aaattgagtt attttctctt ggctaacttc atctgttaat acatagattt
    12241 cttgttgttt atcatccaat gctttttcaa taacattttt caaacctata ttttcttgct
    12301 caaagtacct tccaagtgta tcactatctt cagcatttct caaagaaata gctagatagt
    12361 actcattgaa gatatcagac tgcattgaca acctacacgg acaatgataa gtattcatag
    12421 gcttaatttt tatctgtgat cgaatactac gactaatatc gatgatatcg gctaaaacag
    12481 aggttgctgt tggattttgt cccgcacctg gtccataaaa aagagactga ccaatcccta
    12541 ttgattcaat gaatactgca ttcatcacat gattaactgc agctaaggga tgctcttgag
    12601 aaattagtgt cggagaaacg tctacactta taccagttgg agtttcacga agatcagtta
    12661 ccaacttaat ggcaaagccc atctcctgag ctacttcaac gtcctctgag cgaatactag
    12721 aaatcccctt ataattaaca tcatcaaaat caataggcat accaaatcca aattgactta
    12781 agatagtggc tttgtaggca gtatcaatac cttcaacatc atttgtagga tcactttcgg
    12841 cataacctaa ctcttgtgcc ttttttagag cttgttcata agaccatccc tcttcaaaca
    12901 tttttgttaa catgaagttg gaggtaccgt tgagaattcc taataaacgt gtcattttat
    12961 ctgaggcaaa cgaatgcgat agggtcctta aaataggaat gcctccagca actgctccct
    13021 catagaataa agctagacct ctagcgtctg ctaaatccat caactctttt ccgtgtaaag
    13081 caatgagatc tttattggct gtcacaatat gtttaccaaa tcctaatgct tgactcaaat
    13141 aagttttagc tggctcaatc ccccccatta attccactac aatatcaatt tgtggatctt
    13201 gaagaatagt atttatctct gtcacaaagt tataagtaaa tccctgattg atgaaacgct
    13261 ggcggctttc attatctctt actaatactt tatcaattac aatatcttcg ccttctaaag
    13321 aaagtagctt atgttgattt tcttttagca aatatggaat acccttagca accgttccaa
    13381 aacctaataa agctatttta atagtcatga atttctcctt agaaattgtc tctaacttca
    13441 agacaaacat tggctatatt ataacaaatt accaatattt tttatagggt aaacgcgtct
    13501 tgtcctatga aagttcttaa aaagagaata ttatttcaga aaagatgata caataaaagt
    13561 actttaaaag ttaaggagaa ataaatggca cacacaccta ctagtcatcg taaaccacga
    13621 aaacgttccc catggttagc tattgcttct gtcttttttt tgttaatagc tttaatcggc
    13681 atatttctat tcttcaacaa tcgttcaaag caagaaataa aaacaaaaac gaacgcctct
    13741 agtcatcgaa aaattgtcac atctatcaaa aaaaagaaat gggttaagca aaagactcct
    13801 gttaaaatac ccattttaat gtaccatgct gttcatgtta tggacccttc tgaagccgcc
    13861 agtgctaact taattgtggc acctgacatt tttgaaagtc atataaaacg tctcaaaaag
    13921 gaaggatatt acttcttagc ccctaacgag gcatatcgtg cactaaacga aaatgcttta
    13981 cctgaaaaaa aaagtcattt ggattacttt tgatgatgga aatgccgatt tctacactaa
    14041 ggcataccct atactcaaaa agtacaaggt taaagccact aataatatca tcactggctt
    14101 tgttcaagaa ggacgagaat ctaacctcaa cgttcaacaa atgctagaaa tgaagcaaaa
    14161 cggcatgtca ttccaaggac atacggttac tcaccccaat ctttctctct taactccaga
    14221 actacaaacc caagaaatga cactatcaaa actgttttta gatcaaaaac tctctcaaga
    14281 tactcttgcg attgcttatc catcaggacg ctacaatcct acaactttgg atattgctag
    14341 tcaatattat aaactagggc ttacaacaaa cgaaagagtt gcaacaaaag ataacggttt
    14401 gctttctctt aatcgagttc gtatcttacc aactactagc gatgatgatt tgatcaaaac
    14461 gattaaccaa taaaaagaag ccttctggct tctttttatt cttcataaga tttaaatggt
    14521 tcatttttac gtggataacg atctggagaa atcgataagc ccatttcttt tttctcatgg
    14581 aaaagctcca ctaatagcgc tagcatcatc aaaatcatta cgaatgaaaa tggcagagcg
    14641 acaataatta aaacgttctg cagcgctaac agaccaccag acaagagtaa gacaatagca
    14701 atcactgcca acactagccc ccaaattact ttagttcgat tttttggatt taaattacca
    14761 tcttctgtca acatagccaa aacgtacgtt gcagaatctg ctgatgtaat aaaaaatgaa
    14821 aaaattaaaa tgatagcaat agtggataaa agccaaccta aagtgtagtg attaaaagta
    14881 gcaaacaata cttcctctgt tgcaaacttt gtcaaattaa tacccagttg ttgtacctga
    14941 gttgataatg tgccaaatgc tgcaaaccat acaaaactca ataatgtcgg tattaaaaga
    15001 actaccgtta aaaactcccg aatgctacgc cctttagaaa tacgagcgat gaaaactcca
    15061 acaaaaggag accaggaaat ccaccacgcc caataaaaaa tcgtccaatt atcaatccaa
    15121 gaacgtttag tattgtcaaa agcagctgca cgaaagctca ttccaaagaa attttgtaaa
    15181 taagagccta aagactctgt tagggtatca aaaatacgaa ccgttggtcc caatacaata
    15241 actaaagcta agagggctac cgctaaaatc aaatttaagt tcgataaaat tttaacacct
    15301 tttcctaaac ctgataaagc tgacataaca aataaagctg ttgtaataag gataataaca
    15361 atttgaacaa atgcattatt gggaacaccc aataaaaaac ttaaccctcc gttgatttga
    15421 gcagctccaa atccaagtgt cgtagcaact ccaataactg tagcaacaac tgtggtgata
    15481 tcaacaattt ttcctagtca accatctgtc ttatcaccga aaagaggctt taaagtgaca
    15541 gacaagaggt atttctcttg ctttcgaaat ccaaaataag ctagagccaa agcaaccaat
    15601 gcatatactg cccaagcatg tatcccccag tgaaaaaatg taaaacgaaa tgcatcagct
    15661 aatgctgttt gcgattcctt aggtgcgcca ggtgtcgaaa tagcaaaatg cgataagggg
    15721 tcagctgctc cgtagaagac caaaccaatc cccatacctg ctgaaaacat cattgctatc
    15781 catgatacag ttgaatattc aggacgtgaa tgaggtcgcc ctaaacgtat ttgtccaaga
    15841 ggactcataa taaaaaagac acacaaagca agaattgaag taactaacaa caagtacaac
    15901 caaccgaaat tggtagatac ctgttcacgc aagatttgtg tccaagattg aaaaccacga
    15961 ggaacaataa ttcctaataa gactaaaata agcgatacaa ttagtgaacc tgtaaacaca
    16021 ggcgtaatat gttttttact caaaatttcc tcctatacta aaatagtata gcatacttta
    16081 ataaatactc ctctcatttt aatcacctgt atcaagacct ctccttgtct cctcaacaaa
    16141 aaagaaccag ataacattat ctggttcttg tgtttgactt agtgattaat cactatttgg
    16201 tgtaaatacc aaatgttcgt tgacaaaggt atcaaatcca agttggctca attcacgacc
    16261 atatcctgaa tttttaatac ctccaaacgg taattccggt aatgatgtcc accctgaatt
    16321 aataaatgtc atccctgttt caatctgagc cgccactttt ttagcatgtt ctggatcaga
    16381 agagaaaaca gtacttccta aaccataact agaatcatta gctaaagcaa tagcttcttc
    16441 ttcagtatcc actttataga tagaggctac ggggccgaaa atttcttgat tatagattgg
    16501 atttgcttta gtgatattcg ttaaaacagt tggcatgacg aagtttcctg gatgatctat
    16561 agtgtcattt ccaaaaacta cctcagcacc gtggtctacc gccaatttaa tttgttttaa
    16621 aacatcatcc tttgcacctg cagatgacag tggagccaag gttgtttccg aatccattgg
    16681 gtcaccccat ttagcagact taaaggtctc aactaccata tttacaaatt cgtcatactt
    16741 atcagccata acaatgaaac gttttgaaga tgtacatact tgacctgcat tatataaacg
    16801 cgcaaagaat attgttttac taagtaaatc aaaatcagca tcatctaaaa taagaaaagc
    16861 atcgttccca cctaattcca tagacgattt tttcaaattt ttaccagcct cagctgctat
    16921 tgatgcccca ccacgctcag aaccagttaa acacacacct gcaacacgag gatctgatat
    16981 aaggtttgaa acttgatcat acgaagcaaa aatattttta aacgctcctt ctggagctcc
    17041 tgcttctcgg actaaatcct caaatgcttg agctgatgca ggacaaatag aagcatgttt
    17101 taaaagcata gtattaccca ctataaagtt aggggcaaat acacgcataa tctgataaaa
    17161 tgggaaattc catggctcga cagcaagtaa aacaccgaca gcttgtttca aatagtaagc
    17221 ctctccattt ggactttcta acggaacagg ttttaaaaac ttttgcccat tatcagcata
    17281 atagtcagct atgtctgcac acaagtccac ttctccttgt gcttcagtga aaagttttcc
    17341 catatcttta gtcataactt cagcgtactt atcacggtcc ttacgtaata aatctgctac
    17401 tttatgcaat tgattttggc gttcttcaac attatcttct ttgcgccatg tcttgtagag
    17461 ggcatgcgct atgtctaatg actgttcgag atcactgtca ctgatattat cgaattcatg
    17521 caatacttcg ttagtgtacg gataaattgt tttatatgcc ataatgttcc ctccttttga
    17581 tatcctcatt ataacattta tgaaatcgat tacaaatatt tagctttaga caagatcacc
    17641 aagttccaag gatagcttgc attgttaaac taactataat aatgcctaac caacacgctg
    17701 cacctaaaag aattgatttt cctccagttt taattagttt gctgacatta gtatttatac
    17761 caattgcccc cattgccata acaataaaaa atttagataa aaccttaagt ggctcaaaga
    17821 tacgtaacga aaaacctaat gaagcaacta tcgttgttag caaagaagct aagataaaat
    17881 aaagcacaaa cttcggaaat atcttagtaa cacttacaga ttgttctttc tgcgtttgtt
    17941 tcatatgata aatagataat acaatcgtaa ttggaatgat ggctaatgtt cgtgtcaatt
    18001 taacaatagt agctccgccc aaagtattgc tgtgattgat agcatcccat gcagtagcag
    18061 ttgctgtaac tgacgaggta tcattgacag cagtccctgc aaataatgca aaaccgtgat
    18121 cagagagacc aataaaattc cccagcgttg gaaaaattaa agccgctaag atattgaata
    18181 aaaaaataac cgaaatagct tgtgctactt catcatcttt agcattaata actggtgcag
    18241 tcgctgcaat tgcggatcca ccacaaatag atgaaccaac acctattaaa gtagcaatcg
    18301 ttttatctaa cttaaataac ttttgcaaaa cataagcaat aatcaatgaa atggaaatcg
    18361 tcataataat gatgggtaac gatgatatac caactttcat cacttgcatg aggtttagtc
    18421 caaaacccaa taagacaact gctgtttgta aaatatattt agacgtaaaa gcaataccct
    18481 tattaaacaa ttgtcgattt tgataaaagg atccaacaat tattccaatc aagatagcaa
    18541 agactggtgc tcctattaaa gggagataaa gcccaagcaa ccaagatggt atagcaataa
    18601 taaagcataa tattagtcca ggaatttttt ccttaaataa catgatagtc ctcctcacat
    18661 tgactattat atcacaagct ttaaaacgtg gcaggaaaac cgaaagagga ccagaatatg
    18721 ctctgatcct ctttcattac taattcacaa ttatttagtt actttcataa atggatatga
    18781 tttttcgtta cctaaaatca attttgagcc ctctttttta taggctgttt tttcaccatt
    18841 tttcacaata ttaccataga acgcatttgc ctttttattg atatgcgata ctttaataac
    18901 acttgttaag cgatgtactt taccatccat aactggcaat gtcatttcac cagtattagt
    18961 attatatttt cctccgacag cttgtgcagc actttggaaa cttctatcaa tttgttggtc
    19021 aatttgtttg tcagtgattt ttgtttcatc aaaaatcttt ttacctgctt cacttaaatc
    19081 attgtaagtc aaaccttgat cttttaattg tttttcaaga gctttatcaa taaaagtttt
    19141 tatagcttga tggaatttta cctcatcaat ttgagcagtg actgttatct ttgcctcatc
    19201 gtttttgacg ttcatatgca tattagcgcc cttaaccaaa tcagctgcac taatccccat
    19261 atccgctgcc tgacttccaa gttgttgttc tatttctttc tcaacttgct tctctaaaga
    19321 aactgtacgc catttcccct ctagtgactg acgattattc cagaaataga gaccgcctgc
    19381 acctacaatt agaataagta ctagtacagt taaaaaaact tttaaaaact ttttcatgat
    19441 aaaaactcct tatgataaac ta
//
LOCUS       DATRUK010000035        19316 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.035, whole genome shotgun sequence.
ACCESSION   DATRUK010000035 DATRUK010000000
VERSION     DATRUK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19316
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.035"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..1033
                     /locus_tag="VBE34_001223"
     CDS             <1..1033
                     /locus_tag="VBE34_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO7263943.1"
                     /translation="LAVLILPLVISGLLYYLLKPMVTFLEKRGIKRVTAILSVFTIII
                     LLLIWAMSSFIPMMSNQLRHFMEDLPSYVNKVQMETSSFIDHNPWLKSYKGEISSMLS
                     NISSQAVSYAEKFSKNVLDWAGNLASTVARVTVATIMAPFILFYLLRDSRNMKNGFLM
                     VLPTKLRQPADRILREMNSQMSGYVQGQIIVAITVGVIFSIMYSIIGLRYGVTLGIIA
                     GVLNMVPYLGSFVAQIPVFILALVAGPVMVVKVAIVFVIEQTLEGRFVSPLVLGNKLS
                     IHPITIMFILLTSGAMFGVWGVFLSIPIYASIKVVVKELFDWYKAVSGLYTIDVVTEE
                     RSEEVKNVE"
     gene            1023..2249
                     /locus_tag="VBE34_001224"
     CDS             1023..2249
                     /locus_tag="VBE34_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263944.1"
                     /translation="MLNSEKMIVSIQNQDLEHANKYFEKALKNDPEEILLELGAYLES
                     IGFLPQAKRLYDQIRPNYPEVAINLAQIVAEDGDIEQAFLYLDYISEDSQEYVSALLV
                     MADLYDMEGLTDVAREKLLLASKLSDDPLVTFGLAEMNLSLEHYQEAIEGYASLDNRE
                     ILETTGVSTYQRIGKSYAIIGKFDAAIEFLEKAVDIEYDDLTVFELATILYDQEEYQK
                     ANLYFKQLDTINPDFDGYEYIYGLSLREEHKSEEALRLVQQGLRKNSFDGQLLLLASQ
                     LSYELHDVPSSESYLKQAEKVSENQDEIVMRLSNLYLEEERFEEVLELDNDDLENILA
                     KWNIAKAHKALEMDDSVDYYQSLYNDLKDNPEFLQDYAYILREFGYLDKAQEVGKAYL
                     KLVPDDIEMSEWVNNI"
     gene            2359..4041
                     /gene="alsS"
                     /locus_tag="VBE34_001225"
     CDS             2359..4041
                     /gene="alsS"
                     /locus_tag="VBE34_001225"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEO7263945.1"
                     /translation="MTESHNQYGADLIVDSLINHDVKYVFGIPGAKIDRVFDTLEDKG
                     PELIVARHEQNATFMAQAVGRITGEPGVVIATSGPGISNLATGLVTATDEGDAVLAIG
                     GQVKRGDLLKRAHQSMNNVAMLEPITKYSAEVHDPNTLSETVANAYRLAKSGKPGASF
                     ISIPQDVTDSPVSVKAIKPLSAPKLGSASVLDINYLAQAINNAVLPVLLLGNGASSEG
                     VTAAVRRLLDAVKLPVVETFQGAGIVSRELEDETFFGRVGLFRNQPGDMLLKRADLVI
                     AIGYDPIEYEARNWNAEISARIIVIDVEQAEIDTYFQPERELIGDMAHTLDLLLPAIK
                     GYELPEGSKEYLKGLRNNIENVSDVKFDRDSAHGLVHPLDLIDVLQDNTTDDMTVTVD
                     VGSHYIWMARYFKSYEARHLLFSNGMQTLGVALPWAISAALLRPNTKVISVSGDGGFL
                     FSAQELETAVRLHLPIVHIIWNDGKYNMVEFQEEMKYGRSSGVDFGPVDFVKYAESFG
                     AKGYRVDSKDSFEETLKQALIDAENGPVLIDVPIDYKDNVTLGETILPDEFY"
     gene            4055..4774
                     /gene="budA"
                     /locus_tag="VBE34_001226"
     CDS             4055..4774
                     /gene="budA"
                     /locus_tag="VBE34_001226"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEO7263946.1"
                     /translation="MSETVKLFQYSTLSSLMAGLYKGSLTIGELLTHGDLGIGTVHMI
                     DGELIVLDGKAYQAIGTDGKAEIIQLSDDVTVPYAAVLPHHIQKQFDINAETDNKDLE
                     EMILKNFEGQNLFKSLKIKGTFSRMHVRMIPKSPQHKRFADIASNQPEFTRENVSGTL
                     VGIWTPELFHGVGVKGFHVHFISDDLTFGGHVMDYSLTQGKVEIGKVDQLDQCFPTQD
                     QEFLKANFDLQKLREDIDLSE"
     gene            complement(4828..6483)
                     /locus_tag="VBE34_001227"
     CDS             complement(4828..6483)
                     /locus_tag="VBE34_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEO7263947.1"
                     /translation="MSFDGFFLHHLTNELQEQIEKGRIQKVNQPFDHELVLTIRNNRR
                     NYKLLLSAHPVFGRIQTTEANFQNPQNPNTFTMIMRKYLQGAVIETIQQIENDRILEI
                     IVSNKNEIGDHIKATLVVEIMGKHSNIILIDKNEHKIIESIKHVGFSQNSYRTILPGS
                     TYIAPPKTKAINPFDISDQTLFELLQTNDLSPKNLQQLFQGLGRDTALELSHCLKDNK
                     LNDFRQFFSREYYPSLTEKSFSAVQFSSSHETFQSLGQLLDYYYQEKAEKDRIAQQAS
                     DLIHRVQSELEKNIKKLAKQQDELLATENAEEFRQKGELLTTYLSMVPNNQDIVVLDN
                     YYTNQTIEISLDRALTPNQNAQRYFKKYQKLKEAVKHLKGIISDTKNTITYLESVETS
                     LNHASMEDINDIREELVETGFIKRRAHDKQHKRKKPEQYLASDGKTIIMVGRNNLQND
                     ELTFKMARKGELWFHAKDIPGSHVLIRDNLNPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLNKPSGTKPGFVTYTGQKTLRVTPTQEKIDSLKLKK"
     gene            6842..7846
                     /locus_tag="VBE34_001228"
     CDS             6842..7846
                     /locus_tag="VBE34_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7263948.1"
                     /translation="MKNKGLIATLILLTILVVGELFYNKSEKHLNLSEKQVVKIGILQ
                     YVTHDALDAIEKGVEDGLAQEGYKGKKVKLTVLNAEADQSKIQAMSKQLVNHHNDILI
                     GIATPSAQGLAASTKDTPIIMGAVSDPLGAKLVTNMKKPTTNVTGLSNVVPTKQTVQL
                     IKDITPNVKRIGILYASSEDNSVSQVTEFTKYAQKAGLEVLKYSVPSTNEIKTSMSVM
                     TKKVDAVFVPQDNTIASAFRTVIVAANQANIPVYSSVDTMVEQGSIASVAQSQYGLGL
                     ETAKQAIKVLRGKPVKDVPVKVIDTGKPSLNLKAAKHLGIKIPKKIMKQAEITVKVDD
                     "
     gene            7859..8722
                     /locus_tag="VBE34_001229"
     CDS             7859..8722
                     /locus_tag="VBE34_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7263949.1"
                     /translation="MIISSVSQGLLWGILGLGIYLTFRILKFPDMTTEGSFPLGGAVC
                     VTLMNQGVNPILATILGMLSGMLAGFVTGLLYTKGKIPTILAGILVMTSCHSIMLMVM
                     KRANLGLNEIQTLKDFLPFSNDLNLLVLGLIAILLAISVLIYFLYTRLGQAYIATGDN
                     PDMAKSFGIDTDKMEMLGLIVSNGLIALSGALVSQQDGYADVSKGIGVIVIGLASIII
                     GEVLYSTGLTLFERLIAIVVGSILYQFLITAVIALGFNTNYLKLFSAIVLGICLMVPV
                     LKTKILKGVRL"
     gene            8722..9483
                     /locus_tag="VBE34_001230"
     CDS             8722..9483
                     /locus_tag="VBE34_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7263950.1"
                     /translation="MMMKIIELKEATVQVSNGLAEMKTILDHVNLSIYEHDFITILGG
                     NGAGKSTLFNVIAGTLMLSSGNIYIMGQDVTNLSAEKRAKYLSRVFQDPKMGTAPRMT
                     VAENLLVAKFRGEKRPLVPRKITNYTEEFQKLIARTGNGLDRHLETPTGLLSGGQRQA
                     LSLLMATLKKPNLLLLDEHTAALDPRTSVSLMGLTDEFIKQDSLTALMITHHMEDALK
                     YGNRVLVMKDGKIVRDLNQAQKNKMAIADYYQLFD"
     gene            9780..11441
                     /locus_tag="VBE34_001231"
     CDS             9780..11441
                     /locus_tag="VBE34_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO7263951.1"
                     /translation="MSDIKIIALGGVRENGKNLYVVEVNDSIFVLDAGLKYPENEQLG
                     VDVVIPNLDYLIENKKRVQGIFLTHGHADAIGALPYIIAEVKAPVFGSPLTIELAKLF
                     VKNSTAVKKFNNFHVIDSETEIEFQDAVISFFKTTHSIPESMGIVIGTKEGNIVYTGD
                     FKFDQAARKYYQTDLARLAEIGRDGVLALLSDSANATSNEQVASEYEVGDEIKSVIED
                     AEGRVIVAAVASNLIRIQQVFDAAAENGRRVVLTGFDIENIVRTAIRMKRIHIADENM
                     IIKPKDMTRYEDNELLILETGRMGEPINGLQKMAIGRHRYVQIKDGDLVFIVTTPSIA
                     KEAVVARVENLIYKAGGSVKLITQNLRVSGHANGRELQLLMNLLKPKYLFPIQGEYRD
                     LSAHAGLAQEVGMSADDIYIVKRGDIMVLEKDGFFHSGSVPAGDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVVITVSKKEKKIISKARVNTRGFVYVKKSRDILRESAELVNTT
                     VEDYLSKDTFDWGELKGKVRDEVSKFLFDQTKRRPAILPVVMEVR"
     gene            11498..12286
                     /locus_tag="VBE34_001232"
     CDS             11498..12286
                     /locus_tag="VBE34_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEO7263952.1"
                     /translation="MAFFNIEYHSKVLGTERQVNVIYPDAFEMSDDKIDDCDIPVLYL
                     LHGMGGNENSWQKRTNIERLLRHTNLIVVMPSTDLAWYTNTKYGLDYFDAIAIELPKV
                     LKRFFPNMSDKREKNFIAGLSMGGYGAYKIALLTNRFSHAASLSGALSFDFDLLFNNG
                     NDNTNYWSGIFGDLNNTDNIERHSLRRYVESFDMKTKFYAWCGYEDFLFEANEVAIDE
                     LRQLGLTIDYSNDHGKHEWYYWNQQLEKVLEWLPVDYVKEERLS"
     gene            12376..13086
                     /locus_tag="VBE34_001233"
     CDS             12376..13086
                     /locus_tag="VBE34_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEO7263953.1"
                     /translation="MRTLFRMIFAIPKFIFRLIWNIIWGIFKTVLVIAIILFGLYYYA
                     NHSQSEFANQLSDIIQTGKTFLNFADTNQLKNSFANLATDNVHHSENTRWDKNQATIY
                     IATRDSELASAYKQAISNWNATGAFVLQTTDNPNADIIAKDYSDAKTQAAGVAETERN
                     ALTNRISKVTVKLNTFYLRNKQFGYDHTRIVNTAEHELGHALGLGHNDQQHSVMQSKG
                     SHYGIQEVDIQTLKNLYA"
     gene            13324..13995
                     /gene="rpiA"
                     /locus_tag="VBE34_001234"
     CDS             13324..13995
                     /gene="rpiA"
                     /locus_tag="VBE34_001234"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEO7263954.1"
                     /translation="MDELKKLAGVTAAKYVKNGMIVGLGTGSTAYFFVEEIGRRVKEE
                     GLQVIGVTTSNRTTEQARGLGIPLKSADDIDVIDVTVDGADEVDPDFNGIKGGGGALL
                     MEKIVATPTKEYIWVVDESKLVETLGAFKLPVEVVRYGSERLFRVFKSKGYCPSFRET
                     EGDRFITDMGNYIIDLDLKKIEDPKQLANELDHTVGVVEHGLFNGMVNKVIVASKNGL
                     DILEK"
     gene            14052..15263
                     /locus_tag="VBE34_001235"
     CDS             14052..15263
                     /locus_tag="VBE34_001235"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO7263955.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHDMGHNKSNSHGIDTL
                     IKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPEIVSAMRDKDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADISATVADNFGVDTA
                     MIGESFLQDLV"
     gene            15314..15721
                     /gene="arsC"
                     /locus_tag="VBE34_001236"
     CDS             15314..15721
                     /gene="arsC"
                     /locus_tag="VBE34_001236"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416958.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEO7263956.1"
                     /translation="MEKVRIYHNPNCGTSRNVLAIIRHCGIEPEIIYYLKTPPSKMAL
                     VELLLEMKLSVRELLRTDVPAYEKFNLESSSVTDEEMIDAMIQDPVLINRPIVVTSKG
                     AKLCRPCEEILTILPVKMEKDFVKEDGQIIQSL"
     gene            15760..16569
                     /locus_tag="VBE34_001237"
     CDS             15760..16569
                     /locus_tag="VBE34_001237"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO7263957.1"
                     /translation="MTLLEKINETRDFLQAKGVTAPEFGLILGSGLGELAEEIENPIV
                     VDYADIPNWGQSTVVGHAGKLVYGDLSGRKVLALQGRFHFYEGNTMEVVTFPVRIMRA
                     LACHSVLVTNAAGGIGYGPGTLMLIKDHINMIGTNPLIGENLEEFGPRFPDMSDAYTA
                     TYRQKAHQIAEKQNIKLEEGVYLGVSGPTYETPAEIRAFQTMGAQAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQRIKEDFKGLVKSLVAEL"
     gene            16571..17827
                     /locus_tag="VBE34_001238"
     CDS             16571..17827
                     /locus_tag="VBE34_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEO7263958.1"
                     /translation="MVSRLYYAVKFMIAVLFMTVMAGVGAILMHYVLMFTEWLAFGDS
                     RENTLSLLNSVTPIKRVLSLTLVSFLASLSWYYLQIKPKQITSIKQQVVFKDFSVKKS
                     PYWLHIGHAFLQLIYVGAGGPIGKEGAPREFGAINAGKISDLLALKVLDKRLLIVSGA
                     AAGLSAVYQVPLASVFFAFETLALGISLKNIVTLLVSTFGAASIAQLVISTAPLYHIS
                     KMSLNSQSLAFMFLIVLCVTPIAISFRYLNQKVTERRIKNIKILLSLPVASLIVSVLS
                     IIYPQILGNGNALVQEVFKGTTVSLIAILVVLKMIATLSTLYAGAYGGILTPSFSIGA
                     CLGFLLASISIPLLPHLSIVTSMLVGAAIFLAITMRAPLTAVGLVISFTGQSVITIVP
                     LTIAVLFATAYDYFIRKMRSLYVNPY"
     gene            17811..18521
                     /gene="deoD"
                     /locus_tag="VBE34_001239"
     CDS             17811..18521
                     /gene="deoD"
                     /locus_tag="VBE34_001239"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO7263959.1"
                     /translation="MSIHIEAKQGEIADKILLPGDPLRAKFIAENFLEDAVCFNTVRN
                     MFGYTGTYKGHRVSIMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAINPDIHVRE
                     LVLAQAAATNSNIIRNDWPEFDFPQIADFKLLDKAYHIAKEMDITTHVGSVLSSDVFY
                     SNQPDRNMALGKLGVHAIEMEAAALYYLAAQHNVNALAMMTISDNLNNPEEDTSAEER
                     QTTFTDMMKVGLETLISE"
     gene            18530..19297
                     /locus_tag="VBE34_001240"
     CDS             18530..19297
                     /locus_tag="VBE34_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEO7263960.1"
                     /translation="MTDFSTALKVLVEQYSYHNAFLLLRKHGPLNSDLLFLLEMMKER
                     RELNIDFLFAHQEQVVILQEKYNIKLLHNSYDLELLANYIMDLEAKVKNGLIIDFVRS
                     VSPILYRLFMILLAQEVPHLHDYIYNARDDHYDTWKFKELKESNHPVLLAFSERWHDS
                     RLTSKSLAECLQLTDLDEEVKSTIIQLRQFEKSVRNPLAHLIKPFDEQELYRTTQFSS
                     QAFLDQIIFLAKVIGVEYDTVNFHYDTVNKLIIKILE"
BASE COUNT         6130 a         3052 c         3949 g         6185 t
ORIGIN      
        1 tcttgctgtg ctgatattgc cgcttgtaat ttctggcttg ctttattacc tattaaaacc
       61 tatggttaca tttttagaga agcggggaat taagcgtgta acagcgatat tatcagtttt
      121 tactattata atccttctgt taatttgggc aatgtctagt tttattccca tgatgagtaa
      181 tcaattacgc cattttatgg aagatctccc ttcatatgtg aataaagtgc aaatggaaac
      241 aagttcgttt atagatcaca acccttggtt aaaatcttat aaaggggaaa tatcgagcat
      301 gttatctaat atcagtagcc aagcggtctc ttatgctgaa aaattttcaa agaatgtttt
      361 agattgggca ggaaatttag ctagtacagt tgcacgtgtg acagtagcaa caatcatggc
      421 tccctttatt ttgttttatc ttttaagaga tagtcgcaac atgaagaatg gtttcttaat
      481 ggttttacca accaaactac gccaaccagc tgatcgtatt ttgcgagaaa tgaatagtca
      541 aatgtcagga tatgtgcaag gacaaatcat tgttgctatt actgttggtg ttattttttc
      601 aataatgtat agtattatag gccttagata tggcgtgaca ttagggatta ttgccggtgt
      661 gttaaatatg gttccctatt tgggaagttt tgtcgcccaa attccagtgt ttatcttagc
      721 gcttgtcgca ggacctgtta tggttgttaa agttgcgatt gtttttgtta ttgagcaaac
      781 tctagaggga cgctttgtct cacctttggt tttaggtaat aaacttagca ttcatccaat
      841 tacaattatg tttattttat taacctctgg agcgatgttt ggtgtttggg gagtattcct
      901 cagtattccg atttatgcat ctatcaaagt tgttgttaaa gaattgtttg attggtacaa
      961 agctgtcagt gggctatata caatagatgt tgttactgaa gaaagaagtg aagaagttaa
     1021 aaatgttgaa tagtgaaaaa atgattgttt ctatccaaaa tcaggattta gaacatgcta
     1081 acaagtattt cgaaaaagcg ttgaaaaatg atcctgagga aatcttattg gaactaggag
     1141 cttatttgga gagtattggt tttttacccc aagcaaaacg tctctatgat caaatccgac
     1201 caaattatcc agaagttgct atcaatttag ctcaaattgt tgccgaagat ggagatattg
     1261 aacaagcatt cttatactta gattatatct cagaagatag tcaagagtat gtttcagccc
     1321 tcctcgttat ggctgactta tatgatatgg aaggcttgac tgatgttgcg cgtgaaaagc
     1381 ttttgctagc cagcaaactt agcgatgacc cattggttac atttggttta gctgaaatga
     1441 accttagttt ggaacattat caagaagcga tagaaggata tgcatcactt gacaatcgag
     1501 aaatcttaga aacgactgga gtttcaactt atcagcgtat tggtaaatcc tacgctatta
     1561 tagggaaatt tgatgcagct atcgaatttt tagaaaaagc cgttgacatt gagtatgatg
     1621 atttaacagt ttttgagtta gcaacaatcc tttatgatca agaagaatat caaaaagcta
     1681 acctttattt taaacagttg gatactatta atccagactt tgatggctat gaatatatct
     1741 atggtttatc tcttcgtgag gagcataaat ctgaagaagc tttacgtcta gttcagcaag
     1801 gtcttcgtaa aaatagtttt gatggtcaac ttctcttact agcttcacaa ttatcgtatg
     1861 aattacatga tgttccttcg tcggagtctt atttgaaaca agctgaaaaa gtttctgaaa
     1921 accaagatga aattgttatg agattgtcaa atttgtatct tgaagaagag cggtttgagg
     1981 aagtcttgga attagacaat gatgatttag aaaatatcct tgcaaaatgg aatatcgcta
     2041 aagcacacaa ggctttagaa atggatgata gtgtagacta ttatcaatct ttgtataatg
     2101 atttaaaaga taatccagaa tttttacaag attatgctta cattttgaga gaatttggtt
     2161 accttgataa ggcacaagaa gtaggaaagg cttatttaaa attagttcca gacgatatag
     2221 agatgtcaga atgggtgaat aacatttaaa taggaaaaca cttacagaaa aaaataaaaa
     2281 attcacaagt aaattttcac aatctgatgt aaaaactgtg atataattga accattcttg
     2341 tgaaaagagg gagataaaat gacagaatca cataatcaat atggggctga cttaattgtc
     2401 gatagcctca ttaatcatga tgtcaaatat gtttttggta tcccaggagc taagattgac
     2461 cgcgtatttg acactctaga agataaaggt ccagagttaa ttgttgctcg tcatgagcag
     2521 aacgcgactt ttatggctca ggctgtcggg cgtattacgg gagaaccagg agttgttatt
     2581 gcaactagtg gacctgggat ctcgaacttg gcaacagggt tagttaccgc gacagatgaa
     2641 ggtgatgctg ttcttgcaat tggtggacag gttaagcgag gagatttatt gaaacgtgct
     2701 catcaatcaa tgaataacgt tgctatgcta gaacctatta caaaatattc tgctgaggtt
     2761 catgacccta atactttgtc agaaactgtt gcaaacgcct atcgtcttgc taaatctggt
     2821 aaaccaggag caagttttat ctctattcct caagacgtta cagacagtcc agtctcagta
     2881 aaagcaatca aacctcttag tgcacctaaa ttgggttcgg catctgtttt agatattaat
     2941 tatttagcgc aagctattaa caatgctgtt ttaccggtac tgttattagg aaatggcgca
     3001 tcttcagagg gagtaactgc tgccgtacgt cgacttcttg atgctgttaa actaccagta
     3061 gtagaaactt tccaaggtgc gggtattgtg tcacgtgagc tcgaggatga aacattcttt
     3121 ggtcgtgtag gcctcttccg taatcaacct ggagatatgc ttttgaagcg tgcagaccta
     3181 gttattgcta tcgggtatga ccctattgaa tatgaggcgc gtaactggaa tgctgaaatt
     3241 tctgctcgta ttatcgttat tgacgttgaa caagcagaaa ttgatacgta tttccaaccc
     3301 gagcgagaac tgattgggga catggcacat acacttgatt tacttttacc agctattaaa
     3361 ggatatgaat tgccagaagg ttcaaaagag tatttgaaag gtcttcgtaa taacattgaa
     3421 aacgttagcg atgttaaatt tgatcgtgac tcagctcatg gacttgttca tcctcttgat
     3481 ctcattgatg tactccaaga taataccacc gatgacatga ctgttacagt tgatgtggga
     3541 agccattata tttggatggc tcgctacttt aaatcatatg aagcgcgtca cttgctcttc
     3601 tcaaacggaa tgcaaacatt aggagttgct ctaccttggg caatttctgc ggctcttttg
     3661 cgtcccaaca caaaagttat ttcagtttct ggtgatggtg gcttcttatt ctcagctcaa
     3721 gagttagaaa ctgcagttcg tcttcatcta ccaattgttc acattatttg gaacgatgga
     3781 aaatataaca tggttgaatt ccaggaagaa atgaagtatg ggcgttcttc aggtgttgac
     3841 tttggaccag ttgattttgt aaaatacgct gaaagtttcg gtgctaaagg ttaccgtgta
     3901 gacagcaaag acagttttga agaaacactc aaacaagctc taatcgatgc tgaaaatgga
     3961 ccagtgttga ttgatgtgcc tattgactat aaagataacg tgacactagg tgaaacaatc
     4021 ttacctgacg agttctacta agacgaggtc taatatgtca gaaacagtta aattatttca
     4081 atacagcacc ttgagttcac tgatggcagg attgtacaaa ggcagtctaa ctatcggaga
     4141 attattaact cacggtgact taggaatcgg tactgttcat atgattgatg gtgaattgat
     4201 tgtattggat gggaaagctt atcaagctat tggtacagat ggtaaagcag agattatcca
     4261 attatcagat gatgtgacag taccctatgc agcggtttta ccacaccata tccaaaaaca
     4321 atttgatatc aatgctgaga cagataataa ggatttggaa gagatgattc taaaaaactt
     4381 tgaaggtcaa aatctcttta agtctttaaa aatcaaagga acgttttcaa gaatgcacgt
     4441 tcgtatgatt ccaaaatctc cacaacataa acgttttgca gatattgcat ctaatcagcc
     4501 agaatttacc agagagaatg tatctggtac gttggttggg atttggacgc cggaattatt
     4561 tcatggtgtg ggggtaaaag ggttccatgt ccactttatt tcagacgatt taactttcgg
     4621 tgggcatgtt atggattact cgctcacaca aggaaaagtt gaaattggaa aggttgacca
     4681 acttgaccaa tgtttcccaa ctcaagatca agaatttttg aaagctaact ttgacctaca
     4741 aaagcttaga gaagatattg atttaagtga ataagaatac ctataaataa ttaataacac
     4801 taaaaataga cagggatatg tcccagtcta tttttttagt ttaaggctat ctattttttc
     4861 ttgtgtggga gtgactcgta aggttttttg accagtgtaa gttacaaaac ctggcttagt
     4921 accacttggt ttattgagtt tttttgcttc aatcatgtct acttgaacaa gattggagag
     4981 acgagcttta gagtaataag cagctaactc ggcggcatct gtcttaacct cgtcacttgg
     5041 attgaggtta tcccgaatta acacatggct tccggggata tccttggcat gaaaccacaa
     5101 ctccccttta cgagccattt taaatgttaa ctcgtcattt tggagattat tacgtcccac
     5161 cataataata gtcttgccat cggatgctaa atattgttca ggttttttac gcttatgttg
     5221 tttatcatgt gcgcgtcgct taataaatcc agtttcaaca agttcttcac gaatatcatt
     5281 gatatcttcc atagaagcat gatttagcga tgtttcaaca gattcaaggt aggtgattgt
     5341 atttttcgta tctgaaataa ttccttttaa atgttttaca gcttctttta atttttgata
     5401 ttttttaaag tagcgttggg cattttggtt gggtgttaaa gctcgatcaa gtgaaatttc
     5461 aatcgtttgg ttggtgtaat aattatcaag aacaacgata tcttgattat ttggcaccat
     5521 agagagatag gtagttagaa gctctccttt ttggcgaaac tcctctgcat tttcagtagc
     5581 tagaagttcg tcttgttgct tagctagttt tttgatattt ttctctaact cactttgaac
     5641 acggtgaatg aggtcactag cttgctgtgc tatgcgatct ttttcagcct tctcttggta
     5701 gtaataatct aacaattgtc caagagactg aaatgtttcg tgactgcttg agaattggac
     5761 agcggaaaac gatttttctg ttaggctagg ataatattct ctcgaaaaga attgacgaaa
     5821 atcgttaagt ttattgtctt tcaaacagtg agataattct agcgcagtat cgcgtcctaa
     5881 accttggaag agctgttgga ggtttttagg gcttaggtca ttggtttgaa gcagctcaaa
     5941 tagagtttga tcagaaatat caaaaggatt gattgctttt gttttgggtg gggcaatgta
     6001 agttgaacca ggaaggatag tacggtagga gttttgcgag aaaccaacat gcttaattga
     6061 ttcaataatt ttatgttcat ttttatcaat caagataatg ttactgtgtt ttcccatgat
     6121 ttcaacaaca agtgtcgctt taatgtgatc accgatttcg tttttattag acacgatgat
     6181 ttctaatata cggtcatttt caatttgttg aatggtttca atcactgcac cttgcaaata
     6241 ctttctcatg atcattgtaa aagtatttgg attttgagga ttttggaaat ttgcttcggt
     6301 agtttggata cgtccaaata cagggtgagc agataggagt agtttatagt tgcgacgatt
     6361 attgcgtatt gttaatacca attcatggtc aaaaggttgg ttaacttttt ggatacgtcc
     6421 tttttcaatc tgttcttgta attcatttgt gaggtgatgt aaaaaaaatc catcaaaaga
     6481 catggcgtcc cccttattct aaaaattata gcttatttat tataccatag gctgagctaa
     6541 aaatattttt ttataacgca aaaggcttgc aattttcaga atatagtata taattataga
     6601 aaaattcatg agaaaggatg actagtaaat gactaaaaca ttatttacca attggcaaaa
     6661 ctggcgtaga taaagttgtg tttatgggtg catagatacg actttttgga gtacttcaaa
     6721 aaagtatcta tgcgctggtc tagtctaaca atagagagcg caaggtaagt tatttatcag
     6781 gcgctttttc ttttgtgcta aactccagaa agtcaggata ttattaaagt gaggagaaaa
     6841 tatgaaaaat aaaggactga tagctacgct aattctattg accatattag tggttgggga
     6901 attattttat aacaaatcag aaaaacatct taatttatca gaaaagcaag ttgttaaaat
     6961 tggtatttta cagtatgtaa cccatgatgc tttggatgct attgaaaagg gagttgaaga
     7021 tggtctagct caagaaggat ataaagggaa aaaagttaaa ttaaccgttt taaacgccga
     7081 agctgatcaa agtaaaattc aagcgatgag taaacaatta gttaatcatc acaatgatat
     7141 tttgattgga attgcgacac catcagcaca aggtttagca gcttcgacta aggatactcc
     7201 tataatcatg ggagctgttt ctgatccttt aggggccaag ttagtaacga atatgaagaa
     7261 accaacaact aatgttacag gattatcaaa cgttgttccg accaagcaaa ctgtgcaatt
     7321 aatcaaggat ataactccca atgttaaacg tataggaatt ctttacgcta gtagcgaaga
     7381 taattctgtg tcacaagtaa ctgagtttac aaaatatgct caaaaagcag ggctagaagt
     7441 tcttaaatat tctgtaccat ctacaaatga aattaaaaca agtatgtcag tcatgaccaa
     7501 aaaagtagac gctgtctttg ttccacaaga taatactatt gcgagtgctt ttagaacagt
     7561 tatcgtagca gcaaaccaag ctaatatacc tgtgtattcc agtgttgata ccatggttga
     7621 acaaggaagt atagcatcag tagcgcagag tcaatatggc ttagggttag aaactgcaaa
     7681 acaggccatt aaagtcctaa gagggaagcc ggttaaagat gtaccagtca aagttattga
     7741 tacagggaag ccaagcctca atttgaaagc agccaaacac ttaggaatta aaattcctaa
     7801 gaagattatg aaacaggcag aaattaccgt taaggttgac gattagaaag agggttttat
     7861 gattatttca tcagtttcac aaggtttatt atgggggata ttaggattag ggatatactt
     7921 aacctttaga atcttaaaat ttccagacat gacaacagag ggctccttcc ctctaggggg
     7981 agcggtttgt gttaccttaa tgaatcaagg ggttaatcct attttggcga cgattctagg
     8041 catgctatca gggatgttag cgggatttgt tactggttta ctttatacta aaggaaagat
     8101 accgacgatt ttagcaggga ttttagtaat gacctcatgc cactctatta tgctaatggt
     8161 tatgaaaaga gcaaatctag gtttaaatga gattcaaaca ttgaaagatt ttcttccgtt
     8221 ttcaaatgat ttaaatcttc tcgtattagg tttgatagct attctcttgg cgatttctgt
     8281 cttaatttat tttctctata cacgcttggg gcaagcttat attgcaacag gagacaaccc
     8341 tgatatggca aagagcttcg gcattgatac cgacaaaatg gaaatgcttg gcttaattgt
     8401 atcaaatggc ttaattgctt tatcaggagc tttagtaagt caacaagatg gatatgctga
     8461 tgtatcaaaa ggtataggtg ttattgttat tgggttagct agtattatta ttggagaggt
     8521 actttactct actggtttaa ctctctttga acgtttaatt gctatagtgg tgggttctat
     8581 tctttatcaa tttttaataa cggcagtaat cgccttagga tttaatacga attacctaaa
     8641 attatttagt gcaatagtgc ttggaatttg tttaatggtt cctgtcctaa aaacgaagat
     8701 tttgaaagga gtacgcttat aatgatgatg aaaatcatag agttaaaaga agcaaccgta
     8761 caagtcagta atggtttagc agaaatgaaa acgatattag atcatgttaa tttgagtatt
     8821 tatgaacatg acttcattac aatattgggt ggaaatggag ctggaaaatc aacgcttttt
     8881 aatgtaattg caggtacctt gatgttaagc agtggaaata tttacatcat gggacaagat
     8941 gttactaatt tatcagcaga aaaacgagct aaatatttat cacgggtatt tcaagatccg
     9001 aaaatgggaa cagcacctag gatgacagtt gctgaaaatt tattagttgc taaatttcga
     9061 ggtgaaaaga gaccgttagt tcctagaaag attactaact ataccgaaga atttcaaaaa
     9121 ttgattgcta gaactggtaa tggacttgat cgccatttag agacacctac aggtttatta
     9181 tcaggtggac aaagacaagc gcttagttta ttaatggcaa ctttgaaaaa accaaatctg
     9241 ttattattgg acgaacacac agcagcgctt gatcctagaa caagtgtttc gcttatgggc
     9301 ttgactgatg agtttatcaa acaagattcg ttgactgcct taatgattac acatcatatg
     9361 gaagatgctc tcaaatatgg aaatcgtgta ttagtaatga aagatggcaa gattgtcaga
     9421 gatttaaatc aggcacaaaa aaacaagatg gctatagctg actattatca attatttgat
     9481 taaaaagaag tgaaatggaa gtgtttcact tctttttcta tacactttgc aagaaattat
     9541 tttcaaaata tgatatacta ataatgctgt aaatcgactt gtagagacaa ggaccgtaag
     9601 tacaaatgga atgaaaataa ggacacactc tttaattcat attcctatta ttgattaata
     9661 aggtttttct tcaaacccaa gaaaagttac cgataaatca attactaatg ataataattg
     9721 gacaatttct tgtctgcttt ataaaaaaga attaacaaaa tttaaggaga aaactcatta
     9781 tgagcgacat aaaaatcatt gctcttggag gtgttcgtga aaacggaaaa aacctatatg
     9841 tagtagaagt caatgattct atctttgttt tggatgctgg actcaaatat cctgaaaatg
     9901 aacaactggg tgttgatgtt gttattccaa atcttgatta cttaattgaa aataaaaaac
     9961 gtgttcaggg gattttctta acccatggac atgcggatgc tattggtgca ttgccttata
    10021 ttatcgccga agtcaaagca cctgtgtttg gttcacctct cacaattgaa ttagctaagt
    10081 tattcgttaa aaatagtaca gcagtcaaga aattcaataa tttccatgtg attgatagtg
    10141 aaacggaaat tgaatttcaa gatgcggtga tttcgttctt taaaacgact cattcaattc
    10201 cagagtcaat gggaattgtt attgggacaa aagagggaaa tatcgtttac acaggtgact
    10261 ttaaatttga tcaagctgca aggaaatatt accaaacaga tctggcgcgt ttagctgaaa
    10321 ttggtcgtga tggtgtttta gcacttttat cagattctgc aaatgctaca agcaatgagc
    10381 aagttgctag cgaatatgag gtcggtgatg aaattaagag cgttattgaa gacgcagaag
    10441 gtagagtcat cgtagcggca gttgcaagta atctcattcg tattcaacaa gtctttgatg
    10501 cagcggcaga aaacggtcgt cgagtagttt taacgggttt tgatattgaa aatattgttc
    10561 gtacggccat tcgtatgaaa cgcattcata ttgctgatga aaatatgatt atcaaaccta
    10621 aagatatgac acgttacgaa gataacgaat tattaatcct tgaaactgga cgtatgggag
    10681 agccgataaa tggtctccaa aaaatggcga tcggacgtca tcgttatgtt caaattaaag
    10741 atggtgattt agtatttatc gtaacaacac caagtattgc aaaagaggca gttgtagcgc
    10801 gtgttgaaaa tctgatttat aaagctggtg gctcagttaa attgataaca cagaatttgc
    10861 gcgtatcagg tcatgcaaat ggtcgcgaat tacaattatt aatgaatcta ttgaaaccta
    10921 aatacctttt cccaattcaa ggagagtata gggatttgtc agcacatgcg ggcttagctc
    10981 aagaggtagg aatgtcagca gatgatattt atattgtgaa gcgtggtgac atcatggttc
    11041 ttgaaaaaga tggcttcttc catagtggat ctgttcctgc tggagatgtt atgattgatg
    11101 gaaatgctat tggtgacgtt ggtaatattg tacttcgtga ccgcaaggta ttatctgaag
    11161 atggaatttt tattgttgtt ataactgtca gcaaaaaaga aaagaaaatt atttctaaag
    11221 ctcgtgttaa tacgcgtggt tttgtgtatg tgaaaaaaag cagagatatt ttgcgtgaga
    11281 gcgcagaatt agttaataca acggtagaag attacttaag taaagataca tttgactggg
    11341 gtgaacttaa agggaaagtg cgtgatgagg tcagcaagtt cttatttgac caaacgaaac
    11401 gtcgcccagc aatccttcct gtggttatgg aagttcgttg atagtgttaa gaggttacca
    11461 aattttggta acctttcttg ctagaatgga ggtttttatg gcatttttta atattgaata
    11521 tcactctaag gtactaggaa cggaacgtca agtcaatgtt atttacccag atgcttttga
    11581 gatgtctgat gataaaattg atgattgtga cattcctgtt ttgtatcttc tccatggaat
    11641 ggggggaaat gagaattctt ggcaaaaacg cacaaatata gagcgtcttt tacgtcatac
    11701 taatcttatc gtcgtgatgc catcaacaga cttagcatgg tataccaaca caaagtatgg
    11761 gttggattat tttgatgcaa tagctattga gttaccaaaa gttttgaaac gtttctttcc
    11821 taatatgtct gataagagag agaaaaattt cattgccgga ttatctatgg ggggatatgg
    11881 agcatataaa atagctctac tgactaatcg tttttctcat gctgcctctt tgtcaggtgc
    11941 actttctttt gactttgatt tattattcaa taatggcaac gataacacta actattggtc
    12001 gggaattttt ggtgatttga ataacactga taacatagag cgtcattcac taaggcgtta
    12061 cgttgagtct tttgatatga agactaaatt ttatgcatgg tgtggttatg aagattttct
    12121 ttttgaagct aatgaagtcg caattgatga acttcgtcaa ttaggtttaa ctattgacta
    12181 ttccaatgat catggtaaac atgaatggta ttactggaat caacaattag aaaaagtctt
    12241 agaatggctc cctgttgatt atgtcaaaga agagcgcctt tcttagtatt agctaaatcg
    12301 ttgaaatcca agtaaagtct tgttatacta aatcattaag ttttgggaaa ttagacacag
    12361 aaggtgagat aaaaaatgag aacactattt agaatgatat ttgctattcc aaagtttatc
    12421 tttagattga tttggaatat catttgggga atattcaaga cagttcttgt tattgcgatt
    12481 attttatttg gcttgtatta ctatgcgaat cacagtcaat cagaatttgc taatcaactt
    12541 agtgacatta ttcagacagg aaaaacattt ttaaattttg ctgacactaa tcagctcaaa
    12601 aattcctttg ctaatctagc tacggataat gttcatcatt cagaaaatac tagatgggat
    12661 aaaaatcagg caactatcta tatagcaact agagattctg agttggcctc tgcttacaag
    12721 caagcaatct ccaattggaa tgcaacaggt gcttttgtgt tacaaacaac agataatcct
    12781 aatgctgata ttattgctaa ggattactca gatgctaaaa cacaagctgc aggtgtagct
    12841 gaaaccgaga gaaatgcttt aactaatcgt atttcaaagg taacagttaa attaaacaca
    12901 ttttacctta ggaataaaca gtttggttat gaccatacta ggattgttaa tacggccgaa
    12961 catgaattag gacatgcttt aggtctagga cataatgatc aacaacattc cgttatgcag
    13021 tcaaaaggtt cgcattatgg cattcaagaa gttgatattc aaacgctaaa aaacttatat
    13081 gcataaaaag atgagctaat aaactcatct tttttgtttg aacacttatt ttaggtgata
    13141 taagtgcgtt agcaatagat tattaaaaat aaagaatttg actatcacta ctaactaaaa
    13201 tgcataaaga acaaagaaaa tgttttcaaa aatcacttaa aaaatggtat aatgtgtctt
    13261 gagatagtgt tttgaataga cttatcaaaa atattagata acattaactt aaaaggggaa
    13321 aatatggacg aactaaaaaa acttgctgga gtgaccgctg caaaatacgt taaaaacggt
    13381 atgattgtgg gtttagggac aggttctaca gcatattttt ttgttgaaga aattggacgc
    13441 cgtgtcaaag aagagggatt gcaagtcata ggcgttacaa cgtcaaaccg tacgactgag
    13501 caggcaagag gattaggtat tccgttgaaa tcagcagatg atattgatgt cattgatgtc
    13561 actgtggatg gagcagatga agtagaccct gattttaatg gtatcaaggg gggcggtggt
    13621 gcgctgctca tggaaaaaat agtagctact ccaactaaag aatatatctg ggttgtcgat
    13681 gagagcaaac ttgttgaaac gttaggtgca tttaaattgc ctgttgaggt tgttagatat
    13741 ggttctgagc gtctttttcg ggtttttaaa tcaaaaggtt attgtccttc tttccgtgaa
    13801 acagaaggtg accgctttat cactgatatg gggaactaca tcatcgacct agatttgaaa
    13861 aaaatagagg atccaaagca gttagcgaat gaacttgacc atacagttgg tgttgttgaa
    13921 catggtttat ttaatggtat ggttaataaa gttatagtag caagcaaaaa tggtcttgac
    13981 atacttgaaa aataattatt taaataatcg gttttaggag ctaaccgtaa cctccttaga
    14041 aagaagatat aatgtcacaa tttgatcgca ttcacttagt tgtcttagat tcagtgggta
    14101 ttggagcggc accagatgcc aatgattttg tcaatgcagg agttcctgat ggtgcttcag
    14161 acacgcttgg ccacatttca aaaacagttg gacttgcggt gccaaacatg gctaaaatcg
    14221 gacttggtaa tattcctcgt ccccaagcgc ttaagaccgt ccctgcagaa gaaaatccaa
    14281 gtggctacgc gacgaagtta caagaagtat cacttggtaa ggatacaatg acagggcatt
    14341 gggagatcat gggcttaaat attacagagc catttgatac tttctggaat ggttttccag
    14401 aagatattat cacaaagatt gaggattttt caggacgtaa agttattcgt gaagcgaata
    14461 aaccttattc agggacagct gttattgatg acttcggacc acgccaaatg gaaactggtg
    14521 agctcatcat ctatacctca gctgatccag tcctacaaat tgcagctcat gaggatatca
    14581 tccctctaga agaactctac cgtatttgtg aatatgctcg tagtatcaca atggagcgtc
    14641 cagctctcct tggtcgtatt atcgctcgtc catatgtggg tgaaccaggc aactttacgc
    14701 gtacagccaa ccgtcacgac tatgcagtat caccatttga agataccgta ttaaataaac
    14761 tagatcaagc tggcatcgat acttatgctg ttggtaaaat caatgatatt ttcaatggtt
    14821 caggcattaa tcatgatatg ggacataaca aatcaaatag tcatggtatt gatactttga
    14881 taaaaacgat ggggttatca gagtttgaaa aaggcttctc ctttactaat ttggttgact
    14941 ttgatgctct ctatggtcac cgtcgtgatc ctcatggtta tcgtgattgt cttcatgagt
    15001 ttgatgagcg tcttccagag atcgtttcag cgatgcgtga caaagactta cttttgatca
    15061 ctgccgatca tggtaatgac ccaacttatg caggtacaga ccatacaaga gaatacattc
    15121 ctctcttagc ctatagccca tctttcacag gaaacggcct tatcccagta gggcatttcg
    15181 cagatatttc agcaacagtt gcggataact ttggtgttga tactgcgatg attggagaga
    15241 gcttcttgca ggacttggta taaattttta tttccttaag acacttgtag cattaaaaat
    15301 tgagaggaag atgatggaaa aagttaggat ttaccataac ccaaattgtg ggacatcaag
    15361 gaacgtactt gccattattc gtcattgtgg tattgaacca gaaattattt attatctgaa
    15421 aacaccacct agcaagatgg cactagttga actgttgtta gaaatgaaac tttctgtgag
    15481 agaacttcta agaacagatg ttcctgctta tgagaaattc aatttggaat cttctagcgt
    15541 tacagatgaa gaaatgattg atgcgatgat acaagatcct gttcttatca atcgtcccat
    15601 agttgttacg agtaaaggtg caaaactctg ccgaccctgt gaagaaattt taaccatttt
    15661 acctgtaaag atggaaaaag attttgttaa agaagatggg cagattatcc aaagtttata
    15721 acatattgta tagtgaataa tagtttagga ggtaagtaaa tgacattatt agaaaaaatt
    15781 aatgagacta gagacttttt gcaagcaaaa ggcgtcacag caccagaatt tggtcttatt
    15841 ttaggctctg gtttaggaga attagctgaa gaaatcgaaa atcccattgt tgtggattat
    15901 gcagacatcc caaattgggg acagtcaaca gtagttggtc atgctggaaa attagtgtat
    15961 ggggatttat caggccgtaa ggtattagcg cttcaaggtc gttttcactt ttatgaagga
    16021 aatacaatgg aagtcgttac tttcccagta cgtatcatga gagcattggc ttgccacagt
    16081 gtgcttgtga ctaatgcagc gggtgggatt ggatacggac caggaacttt aatgctgatc
    16141 aaagaccaca tcaatatgat tgggactaac cctctcatag gtgagaacct tgaagaattt
    16201 ggaccacgtt tcccagacat gtcggatgct tatacagcaa catatcgaca aaaagctcac
    16261 caaattgctg aaaaacagaa tatcaaactc gaagaaggtg tgtacttggg tgtatcagga
    16321 cccacttatg aaacacctgc agaaattcgt gcattccaaa caatgggcgc acaagcggta
    16381 ggtatgtcca cggttccaga ggtgatcgtt gcagctcact cagggcttaa agtgttagga
    16441 atttcagcaa ttactaactt tgccgctggc ttccaatcag agctcaatca tgaggaggtc
    16501 gttgaagtta ctcagcgtat taaagaagat ttcaagggat tagttaaatc attagttgct
    16561 gaactttaaa atggtttctc gattatacta tgctgtcaag tttatgatag ccgttctatt
    16621 tatgacagtt atggcaggag ttggagctat cttaatgcac tatgtattaa tgtttacaga
    16681 gtggttagca tttggtgata gtagagaaaa tactctgtct ttgttaaata gtgtcacacc
    16741 aattaaaaga gtgttatcac ttactttagt ctccttttta gcatccctta gttggtatta
    16801 cttacaaatc aagccaaagc aaataactag tattaaacaa caggttgttt ttaaggattt
    16861 ttctgtaaaa aaatcacctt actggctaca tataggtcac gcatttttgc aattgattta
    16921 tgtaggagct ggtggtccta ttggaaaaga aggagcgcca cgagagtttg gagccataaa
    16981 tgctgggaaa atatcggatt tattagcact taaagtgctg gataaacgtt tattaatcgt
    17041 tagtggtgca gctgcaggtt tgtcagcagt ttatcaagta cccttagcta gtgtattctt
    17101 tgcttttgaa actttagctt taggaatttc actgaaaaat attgtgacat tgctagtctc
    17161 tacttttggt gcagctagta ttgctcaatt agttatttcg acagcccctc tttatcatat
    17221 ttcaaagatg agtctgaatt cacagtcatt agcttttatg ttcttgatcg ttttatgtgt
    17281 aactccgatt gcgattagct ttagatattt aaatcaaaaa gtgacagaga gacgtattaa
    17341 aaatataaaa atattgttaa gtttgccagt agcgtcattg atagtatctg ttttatcaat
    17401 aatctatccc cagattttag gaaatggtaa tgccctagtt caagaagtct ttaaagggac
    17461 cacagttagc ttaatcgcta ttttagttgt tctcaagatg atagcaacat tatccactct
    17521 ttacgctggt gcttatggcg gtatactgac accatctttt tccattggtg cttgtttagg
    17581 ttttttgtta gctagtattt ctattccttt acttccccac ctatcgatag taaccagtat
    17641 gttggttggt gcagctattt ttttagctat tacaatgaga gcgccactga cagctgtcgg
    17701 tttagtcata tcttttactg gtcaatctgt tataactatt gttcccctaa ctatagcagt
    17761 actttttgct actgcctatg attattttat aagaaaaatg aggagtttat atgtcaatcc
    17821 atattgaagc aaaacaaggt gaaattgcag ataaaatttt acttccagga gatccactcc
    17881 gtgcgaaatt tattgccgag aattttttag aagatgcggt gtgttttaat acagttcgca
    17941 atatgtttgg ttatacaggt acctataaag gacatcgtgt ttctattatg ggaactggta
    18001 tgggtatgcc ttcaatttca atttatgctc gcgaactaat tgttgattat ggggttaaaa
    18061 cattgattcg cgtaggaaca gcaggtgcta ttaatcctga tatccatgtt cgtgaacttg
    18121 ttttggcaca agcagcagca acaaattcta atattatccg taatgattgg cctgaatttg
    18181 attttccgca aattgctgat tttaaacttt tggataaagc ttatcacatc gcaaaggaaa
    18241 tggatattac aactcatgta ggcagtgtat tatcttcaga cgtgttttat tcaaaccaac
    18301 cagatcgtaa tatggctttg ggtaaactag gtgttcatgc tattgagatg gaagcagcag
    18361 ctctctacta tcttgcagct caacacaatg ttaatgctct tgctatgatg acgatttcag
    18421 acaacttgaa taatccagaa gaagatacaa gtgcagaaga gcgtcaaaca acatttactg
    18481 atatgatgaa agttgggctc gaaacactta tttctgaatg aggatagaaa tgacagattt
    18541 ttcaactgct cttaaagtat tagtggaaca gtattcttat cataatgctt tcttattatt
    18601 acgaaaacat ggtcctttaa actcagattt attattttta ttagaaatga tgaaagagcg
    18661 tcgagagtta aacattgatt ttctttttgc tcatcaagaa caagttgtta tattacaaga
    18721 aaaatacaat ataaagcttt tgcataactc gtatgattta gagttgctgg caaattacat
    18781 catggattta gaagcaaaag taaaaaatgg attaatcata gattttgtcc gtagtgttag
    18841 tcctatttta tatcgtctat ttatgatttt acttgcacaa gaagtacctc acttgcatga
    18901 ttacatctat aatgcaagag atgatcacta cgatacttgg aagtttaaag aattaaagga
    18961 gtcaaaccat ccagtccttt tggcattctc tgaaaggtgg cacgatagtc gcttgacttc
    19021 taaaagcctt gcagaatgtt tacaattaac cgaccttgat gaagaagtga aatcgaccat
    19081 cattcaatta agacagttcg aaaaatcagt cagaaatcct ttggctcacc tgattaaacc
    19141 ttttgatgag caagaactat atcgtacaac tcaattttct tctcaagcat ttttagacca
    19201 gattatcttc ttggcaaagg taattggtgt tgagtatgat actgttaatt ttcactacga
    19261 tacggttaac aagcttatta taaagatact tgagtaattt aaaaacagtt ctgaga
//
LOCUS       DATRUK010000036        19267 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.036, whole genome shotgun sequence.
ACCESSION   DATRUK010000036 DATRUK010000000
VERSION     DATRUK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19267
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.036"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..310
                     /locus_tag="VBE34_001241"
     CDS             <1..310
                     /locus_tag="VBE34_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7263961.1"
                     /translation="VNLVDDLDLELLLTSIDSNIKQYVLNKALVNLSKNDKILLNSYF
                     KHNLSLKECSQELFIHKNTVQYRLNKIYESTQLNPRNFKDATLLYLALKSEDIYLHKL
                     "
     gene            complement(379..1806)
                     /locus_tag="VBE34_001242"
     CDS             complement(379..1806)
                     /locus_tag="VBE34_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEO7263962.1"
                     /translation="MTIFPKHFLWGGAVAANQVEGAFRTDGKGLSVQDVLPNGGLGDF
                     TAKPTPDNLKLEAIDFYHNYKNDIKLFAEMGFKVFRTSIAWSRIFPNGDDSAPNEAGL
                     QFYDNLFDELLKYNIEPLVTLSHYETPLHLAKTYNGWADRRLIAFFEKFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGAIMADKSQLSPQELYQAIHHELVASARVTKLGRSI
                     NPNFKIGCMILAMPAYPMTSDPRDVLAARQFEQHNLLFSDIHVRGKYPTYIQSYFKNN
                     GIEIKFEEGDEEVLAQNTVDFLSFSYYMSVTQAYDFENYQSGQGNILGGLTNPYLTTS
                     EWVWQIDPIGLRLVLNQYYERYQIPLFIVENGLGAKDQLIETLDGDYTVEDDYRIDYM
                     NQHLVQVAKAIEDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGTGSLQRY
                     KKKSFGWYQKVIKTNGQSLFEHHNR"
     gene            complement(1823..3691)
                     /locus_tag="VBE34_001243"
     CDS             complement(1823..3691)
                     /locus_tag="VBE34_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEO7263963.1"
                     /translation="MTKYQETAKAILAAVGGEKNIQHVTHCVTRLRLVLDNDEIVNDQ
                     VIKTIPNVIGVMRKNDQYQIILGNDVNNYYNAFLALGHFENTTREFSSQKKSSIFEKL
                     IETIAGVIKPLIPALLGGGMLKVIGILLPMLGITSSSSQTVAFINFFGDAAYYFMPIM
                     IAYSAASRFKVTPVLAATVGGILLHPAFVTMVAEGKPLSFFGAPVTLASYGSSVIPIL
                     IMVFLMQYIERWINKIVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGKGLSSAML
                     SIYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVTTPDVLILPAMLASNLAQG
                     AASLAVAVKAKQKQTRQVAFAAGLSALLAGITEPALYGVTLKFKKPLYAAMISGGLVG
                     AYIGLVNIASYTFVVPSIIGLPQYINPQGGNNFSNAVIVAIATIILTFIITWFLGIDE
                     GENEKSSINAQEYTHIRSGLSKKETLYSPMVGNVLPLSKVPDETFSSKLLGEGLAITP
                     SVGEVYAPFDGEIISLFPTKHAIALKDDKGVEVLIHIGIDTVELNGEGFEQLVKVGDF
                     VKRGQLLLRMDIDFISSKGYSLISPVVVTNSIDQLEIIVKDEETMVTNEDDLLVIL"
     gene            complement(3684..4535)
                     /locus_tag="VBE34_001244"
     CDS             complement(3684..4535)
                     /locus_tag="VBE34_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO7263964.1"
                     /translation="MIIKRVLNHNAVISVTHQGLDVLLMGKGIAFKKRIGDRINSDAI
                     EKSFVLKNSDNMNRFTELFITVPEEVVACSERIINLGKIKLGKNLDEILYINLTDHIH
                     SAIERHEQRMVIQNPLRLEIQRYYPDEYSIGMKALELIKDELGICLTIDESAFIAMHF
                     VNAGLDNPFNEAHKITEIVSYIEQKVKIDFRTELDESSIDYYRFMTHTKLFAQRVLSG
                     MKYEDDDADLLLVVKKKYPREYKCVKEIGNNMAIQYQYQLNSSELLYLTVHVKRLVKN
                     LKEISDD"
     gene            complement(4873..5481)
                     /locus_tag="VBE34_001245"
     CDS             complement(4873..5481)
                     /locus_tag="VBE34_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEO7263965.1"
                     /translation="MAIILPDLPYAYDALEPHIDAETMTLHHDKHHATYVANANAALE
                     KHPEIGEDLEALLADVSQIPEDIRQAVINNGGGHLNHALFWELMSPEETQISQELSED
                     IDATFGSFEDFKAAFTAAATGRFGSGWAWLVVNAEGKLEVLSTANQDTPIMEGKKPIL
                     GLDVWEHAYYLNYRNVRPNYIKAFFEIINWNKVNELYQAAKA"
     gene            complement(5561..6598)
                     /locus_tag="VBE34_001246"
     CDS             complement(5561..6598)
                     /locus_tag="VBE34_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEO7263966.1"
                     /translation="MIAIEEIGRITPDNLGLVTVLAGEDLGQYAQMKEKLFQVIGFNK
                     DDLAYSYFDLSEEEYQNAELDLESLPFLSDYKVVIFDQFQDITTDKKTYLDEQAMKRL
                     EAYLQNPVDTTRLVICAPGKLDGKRRLVKLLKRDARVLEANTLKESDLKTYFQKYAHQ
                     EGLVFEAGVFDELLIKSNYDFSDTLTNIAFLKSYKTDGHISSNDVREAIPKSLQDNIF
                     DLTQDVLLGRIDLARDLVRDLRLQGEDEIKLIAIMLGQFRMFLQVKILASKGKSESQI
                     VSELSHYIGRKINPYQVKFAVRDSRNLPLAFLKEAIRILIETDYAIKRGTYDKDYLFD
                     LALLKIAHKKP"
     gene            complement(6652..7380)
                     /locus_tag="VBE34_001247"
     CDS             complement(6652..7380)
                     /locus_tag="VBE34_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7263967.1"
                     /translation="MFHREDFSKALLKKALQPGMRVMDIGCGSGELTRLAADIVGKEG
                     DVVGIDINEQLLWQARHDNGYANVSYQNADLAALSSFETKSFDAIIGRRVLMYQKNPK
                     KVIESLTPLLKQNGFYVFQESDAMCLELAKPSFVNMNKIQSLIWQTVEKEGGHIHIGS
                     QLEQLMLEAGLIVDHFVSENVIQTSQTGTDLSWLLNIMKERIQKYSDLQGLEDVNLQK
                     ELEENNDHFIRDCQFGIISHRVDE"
     gene            7556..8548
                     /locus_tag="VBE34_001248"
     CDS             7556..8548
                     /locus_tag="VBE34_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7263968.1"
                     /translation="MKNYRKLIVLLLLIFFAIFMGAYAYTHIVEKRSLTSNTIEKTLP
                     VVNQIKPQTIKEYQNYLTKVAKRNVLPVDIPQALNNEKVEITATDGMQTFTWNDKNNP
                     KQKVIFYVHGGSYIHQASELQYIFVNKLAKKLDAKVVFPIYPKAPTYNYSDAIPKIKK
                     LYQNTLASVTSHKQIILVGESAGGGLALGIADNLVTEHIKQPKEIILISPWLDIATNN
                     PKIEKVQKKDPLLKAWQLQQVAPYWANGKKNFKNPQVSPLYSSQFNKMAPISFFIGTH
                     DIFYPDNQLLHQKLAKENIKHHYIVGQKMNHVYPVLPIPEAETAFKQMLKIINH"
     gene            complement(8607..9551)
                     /locus_tag="VBE34_001249"
     CDS             complement(8607..9551)
                     /locus_tag="VBE34_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7263969.1"
                     /translation="MTSISDIAKKAGVAKSTVSRVINHHPHVSDETRQKVMALITELD
                     YIPNQLARDLSRGKTQKIGVVIPHTRHPYFTQLINGLLDAAKTTDYQLVVMPSDYNQE
                     LELSYLKQLKMEAIDALIFTSRAISLDIIETYAKYGRIVVCEKLQEYNHLSSAYLDRY
                     SSFLEAFSDMKLRGLEHLVLLFSRNNESSATYQSALLAYQEVYGQLSSPYMVVGNVHD
                     FNDGLNLSYQLVKEVSIDGILATSDEVAAGLIKGYEESRKKCPYIIGQECLLVGQLLK
                     LPTIDHKSYYLGKLAFKQALAEKISQEVLFSEFLSQDN"
     gene            complement(9562..10371)
                     /locus_tag="VBE34_001250"
     CDS             complement(9562..10371)
                     /locus_tag="VBE34_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7263970.1"
                     /translation="MTIKRIFCDMDGTLLNSEGQVSKSNATLIREAAIPVTLVSARAP
                     MEMKDAVDALQLGGVQVAFNGGLIYRIGDNNQVLPIHTQIIKKSTVKQLLRGIRFHFP
                     QVSLSYYDLNNWYCDKIDEGIRYEHSLTQQCPTFIHNEDQFLEGHTNTFKIMMITFDE
                     ANMLELEKYLQSLELPEITIQRSGKAYLEITHLLAKKSKGIAYILQKEQLAREETAAF
                     GDGHNDLPMLEMVGYPIVMDNAFDDIKAIAYQLTKSNDEDGVGYGIQKFLK"
     gene            complement(10497..12734)
                     /locus_tag="VBE34_001251"
     CDS             complement(10497..12734)
                     /locus_tag="VBE34_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEO7263971.1"
                     /translation="MLHLTKYFPLKPIYLALLVFQIYLLVFSWTMLGCAFLLFTFIFL
                     IYQYDRETIFKTITIVIFFLFYFLWQNHNMNVQYQRVPNHISQIKVRIDTISINGDVL
                     SFQADASGNTYQAFYTLKNKSEKDYFQNLDNNIMIIADIKLEEAEERRHFNGFDYRQY
                     LKRHGIYRIAKVTKIKQIRLFQHRSFFDLMFKWRRSAIVISQTFPNPMRHYMSGLLFG
                     YLDKTFDDMSDLYSSLGIIHLFALSGMQVGFFLGIFRYICLRIGLRLDHVWLLQIPFS
                     LIYAGLTGFSISVVRALIQSLLSHSGVKKDENFALCLLICLISLPHSLLTTGGVLSFA
                     YAFILTMTSFDHFSSIKKVAIESLTVSVGILPILTYYFSGFQPISIILTALLSFAFDI
                     IFLPLLTVIFVLSPFVKLSCINSLFEILEVLLKWTGQLFPRPLIFGKPSLFLLIVMII
                     ILGLLYDYYHSKCFRYCSLLIIFTLFFITKNPITNEVAILDVGQGDSILVRDWLGKTI
                     LIDTGGRVRFEQPEEWKQKVNQSNAERTLIPYLKSRGISKIDNLVITHTDTDHMGDME
                     VISKHFKVARLITSSGSLTNSQYVKRLSKIGVAVKSIEAGDKLSVMGSYLQVLYPWHK
                     GDGKNNDSIVLYGHLLGKGFLFTGDLEEEGEKQLLEAYPNLSVDILKAGHHGSKGSSS
                     LSFLKKLSPSVVLVSAGKNNRYQHPHQETLQRFQKIKSKIFRTDQSGTIRLTGWWKWH
                     IQTVR"
     gene            complement(12718..13371)
                     /locus_tag="VBE34_001252"
     CDS             complement(12718..13371)
                     /locus_tag="VBE34_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEO7263972.1"
                     /translation="MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDDLN
                     INLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGAVNHPGVYSLPSQSRVTDAIKRA
                     GGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
                     ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTLD"
     gene            complement(13471..14211)
                     /locus_tag="VBE34_001253"
     CDS             complement(13471..14211)
                     /locus_tag="VBE34_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEO7263973.1"
                     /translation="MFYTYLRTLVMFLIWVANGNAHYHNEDKMLKDDENYILVAPHRT
                     FWDPVYMAFAARPKQFVFMAKKELFTNRLFGWWIKMCGAFPIDREKPGQDAIRYPVKM
                     LKNSNRSLVMFPSGSRHSKDVKGGVAVIAKMAKVRIMPAAYRGPMVFKNLLKGHRVDM
                     NFGNPIDVSDIKRMDAEGIAEVSRRIQEEFDRLDRENETYDDGKKLNPLTYIYRLPLA
                     IIAIVLLVLTLIFSYLASFVWDPQKHLK"
     gene            14346..15110
                     /locus_tag="VBE34_001254"
     CDS             14346..15110
                     /locus_tag="VBE34_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEO7263974.1"
                     /translation="MIDTILKENERIDQLFSTDVKIIQNKEVFSYSIDSVLLSRFPKL
                     PSRGLIVDLCSGNGAVGLFASTKTSATIIEIELQERLADMAKRSIKLNKLEKQVTMIN
                     DDLKNLLDHVQRSNVDLMLCNPPYFKASETSKKNLSPHYLLARHEITTNLREICQIAQ
                     HALKTKGRIAMVHRPDRFLEIIDTMRQFNLAPKRIQFVYPKLGKDANMLLIEAIKDGS
                     TEGMKILPPLVVHQDNGDYTETIFDIYFGENGKSHD"
     gene            15103..15369
                     /locus_tag="VBE34_001255"
     CDS             15103..15369
                     /locus_tag="VBE34_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO7263975.1"
                     /translation="MIKVPAYMYVLECSDGTLYTGYTTDVKRRLNTHNTGKGAKYTRA
                     RLPVKLLYSEAFNSKQEAMRAEALFKQKTRKAKLTYIKQHKNEQ"
     gene            complement(15552..17138)
                     /locus_tag="VBE34_001256"
     CDS             complement(15552..17138)
                     /locus_tag="VBE34_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7263976.1"
                     /translation="MKFTELNLSQDILSAVEKAGFVEPSPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIHTEDNTIQALIIAPTRELAVQSQEELFRFGRDKGVKVRS
                     VYGGSSIEKQIKALRSGAHVVVGTPGRLLDLIKRKALKLNHIETLILDEADEMLNMGF
                     LEDIEAIISRVPEARQTLLFSATMPDPIKRIGVKFMKDPEHVKIKATELTNVNVDQYY
                     VRVKENEKFDTMTRLMDVDQPELSIVFGRTKRRVDELTRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDHIDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVSPNEMGYLTIIENLTKKRMTGMKPATASEAFQAKKKVALKRIARDFEDQ
                     ELVSKFDKFKADALELATQYTPEELALYVLSLTVQDPESLPEVEITREKPLPFKPSGG
                     GFKGKGGRGNGRGGDRRRNDRGDRRGNRDRDDRGGRRDFKRRDDKFKKDNRRQENKKP
                     HKNTSSEKQTGFVIRNKGDK"
     gene            complement(17373..18221)
                     /locus_tag="VBE34_001257"
     CDS             complement(17373..18221)
                     /locus_tag="VBE34_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263977.1"
                     /translation="MKKLLSLTCLIMMSLCLVACTKQAMSSKQAMSSKQIKDKNSKEK
                     VITVATYSKPTSTFLDLIKDNVKEKGYTLKVVMVSDYIQANIALENKEHDANLLQHEF
                     FMSIFNKENDGHLVSITPIYHSLAGFYGQHLKNIAELKDGAKVAIPSDPANMTRALLL
                     LQEKKLITLKNTSKKTKAIEDIITNPKKLRIEPVALLNLNQAYFEYDLVFNFPGYVTK
                     INLVPKRDRLLYEKKPDIRFAGALVAREDNKNSDKIKVLKEVLTSKEIRHYITKEIPS
                     EADVAF"
     gene            complement(18237..18899)
                     /locus_tag="VBE34_001258"
     CDS             complement(18237..18899)
                     /locus_tag="VBE34_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7263978.1"
                     /translation="MIDIIEKHGEGILKSFWETNLMLGLTLILCFLIAFPTGILLFSL
                     RKSYLIKHSLAYQLLNLFLGTLRSVPFLIFIFILIPLNRLIFGTSFGTIAAILPLTLV
                     SVSLYARYVEQALLNIPQVVVDRALSLGANKRQIIYYFLIPSIKIDLVLSFTATAISI
                     LGYSTIMGVIGAGGLGEYAYRFGYQEYDYPVMYLIVVLFIIYVFILQSLGYFIANRYS
                     RK"
     gene            complement(18892..>19267)
                     /locus_tag="VBE34_001259"
     CDS             complement(18892..>19267)
                     /locus_tag="VBE34_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7263979.1"
                     /translation="LDFLGLMDLKQVKCHSLSDGQQQKVAIAMAVLQKPKLILCDEIS
                     SALDTNSEKEIFNLLSDLREKYGISILMIAHHLSLLKQYCDRVMILDHQTIVDTVVPV
                     KATLNQLKSNYVDQVKEFLLHD"
BASE COUNT         6400 a         3669 c         2883 g         6315 t
ORIGIN      
        1 ggtcaacctc gtcgacgacc ttgatcttga actgttatta acaagtattg attctaatat
       61 taaacaatat gtgctaaata aagcgcttgt aaacttatct aaaaatgata agatcttgct
      121 taactcatat tttaagcata atctatcatt aaaagaatgt agtcaggaac tttttatcca
      181 taaaaatact gttcaatata ggttaaataa gatttatgaa tcaacgcaac tcaatccaag
      241 aaactttaag gatgcaactt tattgtatct agcacttaaa tcagaagata tttaccttca
      301 taaattataa taaaaaggag taacctatgg atactccttt ttatattaaa ttgaaacaga
      361 gactagttgt ttaacatgtt atctgttatg atgttcaaac aatgattggc catttgtctt
      421 aatcactttt tggtaccaac caaatgactt cttcttgtaa cgttgtagac tccctgttcc
      481 atcatcattt cgatcaacat atattagacc ataacgttta cttagttgag ctgtagacat
      541 ggatacacaa tcaatgcatc cccaagatgt atatcccatt atttcaacac catcttcaat
      601 agctttagca acttgaacta aatgttgatt catataatca atacgataat catcttcgac
      661 agtataatca ccgtctagtg tttctatcaa ttgatctttt gctcccaatc cattttctac
      721 aataaacaac ggaatctgat aacgttcata atactgattt aagactaggc gtagccctat
      781 gggatcgatt tgccataccc attctgaagt tgttaaataa ggattagtca ggcctccaag
      841 gatatttccc tgaccacttt gataattttc aaagtcataa gcttgtgtca cgctcatata
      901 gtaactaaac gataggaaat ctactgtatt ttgtgccaat acttcctcat ctccttcttc
      961 aaatttaatt tcaataccat tatttttgaa ataggactga atataagtgg gatatttacc
     1021 tctgacatgt atatctgaaa atagcagatt atgttgttca aattgtcttg cagccagaac
     1081 atctcttgga tcagatgtca taggataagc tggcatagcc agaatcatac agccaatttt
     1141 aaaattaggg ttgatagaac gccctaattt tgtcactcta gccgatgcta ctaattcatg
     1201 atgaatagct tgataaagct cctgaggact tagttgtgat ttgtcggcca taatagcacc
     1261 actggtgaaa ggcatatgta aaatagaatt cacttcatta aaagttaacc aatattttac
     1321 cttatcttta taacgctcca tgactgtttg agcaaatttc tcaaaaaatg ctatcaaacg
     1381 tctatcagcc caaccattat aggtctttgc aagatgaagc ggtgtttcat aatgagataa
     1441 ggtaaccaaa ggctcgatat tatatttaag caactcgtca aataaattat cataaaattg
     1501 taagccagct tcgtttggag cactatcatc accatttgga aaaattcgag accaggcaat
     1561 agaggttcta aaaactttaa agcccatctc tgcaaacagt tttatatcat ttttatagtt
     1621 atgataaaaa tcaattgctt ctagcttcaa attgtctgga gtaggtttgg cggtaaaatc
     1681 gcctagacca ccatttggaa gtacatcttg tacagataaa cctttgccat ctgttctaaa
     1741 tgcaccctct acctgattgg cagcaactgc acctccccat agaaaatgtt taggaaaaat
     1801 cgtcatatta tcatccttcc ttttaaagaa ttacaagtaa atcatcttca tttgtaacca
     1861 ttgtctcttc gtctttaacg ataatttcaa gttggtcaat tgaatttgta actacaactg
     1921 gactaataag agaatacccc tttgaactaa taaaatcaat atccattcgc aaaagtaact
     1981 gtcctcgttt aacaaaatcg ccaactttaa ccaattgttc aaaaccttct ccatttagtt
     2041 ctacagtatc tattccaata tggattaaca cttcaactcc cttatcatcc tttaaggcaa
     2101 tggcatgttt tgtaggaaat aaacttataa tttcaccatc aaatggtgca taaacttcac
     2161 ccacactagg agtgattgct aaaccttcac ctagtaattt agatgaaaat gtttcatcgg
     2221 gtactttaga taaaggtaat acattgccta ccataggtga atacaatgtc tctttttttg
     2281 ataaaccact tctaatatgt gtgtactctt gagcattaat actacttttt tcgttttctc
     2341 cttcatcaat tcccaagaac catgtaataa tgaaagtcaa aataatagta gctattgcta
     2401 caataacagc attactgaaa ttattgcccc cctgtggatt gatgtattgt ggcaaaccaa
     2461 taatagatgg cactacaaat gtataggaag caatattaac taatccaata taagctccta
     2521 ctaaaccacc tgatatcatt gcagcgtata aaggcttctt gaatttcagt gtgacaccat
     2581 aaagtgcagg ttctgtgata ccagctagta gtgctgataa acctgctgca aaggccacct
     2641 gacgtgtctg tttttgttta gccttaactg caacagcaag ggaagctgca ccttgagcta
     2701 aattagaagc taacattgct ggtaaaatca aaacatctgg tgtcgtaaca gaagcagcta
     2761 aaaagattgg agcaaatgcc cagtgcattc ctgtcataac aaccaatggc ataatagcac
     2821 caagaatagc taaagctaac catggtgcca catggtaaat cgaaagcatt gcactagaca
     2881 atcctttacc aataattact cccagtggcc ccacgaccac taaggctaaa aagccagaaa
     2941 tgagaataat caaagtcggt tgtaaaaagc tcttcattac actaggaaca attttattaa
     3001 tccatctctc aatgtattgc ataagaaaaa ccataattaa gattggaata acagaagaac
     3061 cataactagc cagtgtaacc ggagctccaa agaaagataa tggttttcct tcagctacca
     3121 ttgtcacaaa agcagggtgt aagagaatcc ctcctactgt agcagctaat acaggtgtaa
     3181 ctttaaatcg tgacgcagct gaataagcaa tcattattgg cataaaataa taagcggcat
     3241 caccaaaaaa attgataaaa gctactgttt gagaactaga gcttgttata ccgagcatag
     3301 ggagcaaaat acctataacc ttgagcatac ctcctcctag aagagcaggt ataagcggct
     3361 taatgactcc tgcaatcgtt tcaattaatt tctcaaatat tgaactcttt ttttgtgatg
     3421 aaaattctct ggtagtattt tcaaaatgac ctaaggccaa aaaagcatta taataattat
     3481 tgacatcatt tcccaaaata atttggtatt gatcattttt acgcataaca ccgataacat
     3541 tcggaatagt tttaataacc tgatcgttga caatttcatc attgtctaaa actaatcgta
     3601 atcgtgtcac acagtgggtt acgtgttgaa tatttttttc tccccctact gctgctaaaa
     3661 tagctttagc agtctcttgg tatttagtca tctgatatct cctttaaatt tttaactaat
     3721 cgtttaacat gcaccgtcag atataggagt tcacttgaat taagttgata ttggtattgg
     3781 atagccatat tgttgccgat ctctttaaca catttatatt ctctcggata ttttttctta
     3841 actaccaaaa gtaaatcagc atcatcatct tcgtatttca ttcctgataa aacgcgttga
     3901 gcaaataatt tggtatgtgt catgaaacga taataatcaa ttgaactttc atctagttct
     3961 gttcgaaaat ctatttttac tttctgttca atgtaagata caatctcagt gattttatgc
     4021 gcttcattaa acggattatc caatccagca tttacaaagt gcatagcaat aaaagctgac
     4081 tcatcaattg ttaaacaaat acccaattca tcctttataa gttccagagc cttcatccca
     4141 atggagtatt catcaggata gtaacgctgg atttccaacc ttagtggatt ttggataacc
     4201 attctttgtt catgtctttc aatagccgaa tgaatatgat ctgtcaaatt gatataaaga
     4261 atttcatcta agttcttacc caatttgatt ttacctaggt tgataattcg ttctgaacat
     4321 gcaactactt cttctggaac agtaataaat aactccgtaa aacggttcat gttatcacta
     4381 ttttttaaaa caaatgactt ctcaatagca tcgctattaa tcctatcacc tattcttttc
     4441 ttaaatgcaa ttcctttccc catcagtaac acatctaatc cttgatgtgt tactgatatg
     4501 actgcattat gatttaatac gcgttttata atcattaaag tcctagtcct tcactcgcac
     4561 aataaaaaat gacccatcca agacacaaca aaaagtcgtc gttgaatagg tccctgctca
     4621 caaaatgtga taacatcctt tattatgcaa ttttatctgt tatcagcttg cttaccttaa
     4681 ataataacac ctgaaaacgt cttaatttta tcatttctta ctaatcttgt caagcgcttt
     4741 caataaactt attcgcttaa ttgaagataa taggactgtt ctagttctat ctgagtttgg
     4801 atttctagac ttttaaaaac attatcaact tttagaataa taaaaaaacg agcctatcga
     4861 ctcgtttttt tattaagctt tagcagcttg gtaaagttca tttactttat tccagttaat
     4921 aatttcaaag aaagctttga tgtagttagg acgaacatta cgatagttta agtaataagc
     4981 atgctcccat acatcaagtc ctaaaatagg tttcttacct tccataattg gagtatcttg
     5041 attggcagtt gaaagcactt caagtttgcc ttcagcatta acaacaagcc aagcccaacc
     5101 tgaaccaaaa cgtcctgttg ctgctgctgt gaaagcagct ttaaagtctt caaatgaacc
     5161 aaaagttgca tcaatgtctt cagataactc ttgtgaaatt tgagtttctt ctggtgacat
     5221 caattcccag aaaagagcgt ggttaagatg tccaccaccg ttattgatga ctgcctgacg
     5281 aatatcttct ggaatttgag aaacatcagc taagagcgcc tctaagtctt ctccaatttc
     5341 aggatgtttc tcaagagcag catttgcatt agcaacataa gttgcatggt gcttatcatg
     5401 atgtagtgtc attgtctcag catcaatatg tggctcaagc gcatcatatg catagggtaa
     5461 atctggtaaa ataattgcca tgtcgaaatc ctcttttcat ctattgttac ccttattcta
     5521 taggaatttg gaacagcttt caactctttt gtttctttaa ttagggcttt ttatgagcaa
     5581 tttttaataa ggctaaatca aagagataat ccttatcata agtccctctt ttaatagcat
     5641 agtcagtctc gattaaaatc ctaatcgctt cttttaaaaa agcaagggga aggtttctag
     5701 aatctctgac agcaaatttt acttgataag ggttgatttt acgtcctatg taatgggata
     5761 gttcagaaac aatttgactc tctgactttc ctttactagc caatatttta acttgcaaaa
     5821 acatccgaaa ctgtcctaac atgattgcaa tcaatttgat ttcgtcttcg ccttgtagac
     5881 gtaaatctct tactaaatca cgcgctaaat ctatcctacc aagtaagaca tcttgagtca
     5941 aatcaaatat attatcttgc aggcttttag ggatagcctc tctaacatca ttagacgaaa
     6001 tatgaccgtc tgttttatat gactttaaga aagcgatatt agttaaagta tcactaaaat
     6061 catagttaga cttaatcaat aactcatcaa aaacacctgc ttcaaaaact aatccttctt
     6121 ggtgagcata tttttgaaaa tacgtcttta aatctgattc ttttaaagta ttagcctcta
     6181 aaacacgagc atcacgtttc aataacttta caagtcgacg ttttccatca agttttcctg
     6241 gagcacaaat aacaagtctc gtagtatcta caggattctg caagtaggct tccaagcgct
     6301 tcattgcttg ttcatctaaa taagtttttt tatcagttgt aatatcttga aactgatcaa
     6361 aaataacaac cttgtagtct gataaaaaag gtaaactttc taagtctaat tccgcatttt
     6421 gatattcttc ttcacttaaa tcaaaataag aataggctaa atcgtcttta ttgaatccaa
     6481 ttacctgaaa tagcttctct ttcatctgag catattgccc taaatcttct cctgctaaaa
     6541 cagttactaa ccctaaatta tctggggtaa ttctcccaat ttcttcaatc gcaatcatat
     6601 tccttattat accataagga aacttttcaa ggtgtcaaat aatacttaaa atcattcatc
     6661 aactctatga gaaataattc caaattgaca atctcggata aaatggtcat tattctcttc
     6721 taattctttt tgtaaattga catcttctaa cccctgaagg tcactatatt tttgtattct
     6781 ttctttcatg atatttagta gccaagatag atctgtacca gtttgtgacg tttgaatgac
     6841 attttctgat acaaaatggt caacgattaa gcctgcttct aacattaatt gctcaagttg
     6901 acttccaata tgaatgtgtc ctccttcctt ctcaacagtt tgccagatta gtgattgtat
     6961 tttattcata tttacaaagc taggcttagc tagttctaaa cacattgcat cactttcttg
     7021 aaaaacataa aaaccatttt gctttaacaa aggtgttaaa ctttctatta ctttctttgg
     7081 atttttttga tacattaata cacgacgtcc aataatagca tcaaaacttt ttgtttcaaa
     7141 ggaggaaagt gctgctaaat ctgcattttg ataactaaca ttagcatagc cattatcatg
     7201 tctagcttgc cataaaagtt gctcattaat atcaattcct acaacatcac cttctttacc
     7261 aacgatatca gcagccaaac gcgttaattc cccactacca caaccaatat ccataactct
     7321 cataccgggc tgcaaagctt tttttaataa tgcttttgaa aaatcttctc tgtgaaacat
     7381 aataacactc tcctctcttc tattttagca aattaaaggt tcatacgtac tatcagcaaa
     7441 aaattataat aggttgaaat tttatagcat tattttcttg ttttcgtatc tactttatta
     7501 tagaataaca actaatcatt tttagcatga cgagctcaat tacaaaagga taatcatgaa
     7561 aaactatcga aaacttattg tactactact tctaatcttt tttgccattt ttatgggagc
     7621 atatgcttac acgcatattg ttgaaaaaag atccctaact agcaatacta ttgaaaaaac
     7681 tctacctgtg gtaaatcaga ttaagcctca aaccattaaa gaataccaaa attacttaac
     7741 taaggtagct aaacgtaatg ttcttcctgt agacattcct caggcattaa ataatgaaaa
     7801 ggtagaaatt actgctactg atggcatgca aacattcact tggaatgata aaaataatcc
     7861 taagcaaaag gttatcttct atgttcatgg aggatcatat atccatcaag cttccgaatt
     7921 acaatatatt tttgtcaata aactagctaa aaaattagat gcaaaagttg tctttcctat
     7981 ttaccctaaa gctcctacat ataattatag tgatgctatc cccaaaatta aaaaattata
     8041 ccaaaataca ttagctagcg tcacatctca caaacagatt atcctagtag gtgaaagtgc
     8101 aggcggaggc cttgctttag gtattgctga taaccttgtc acggagcata tcaaacaacc
     8161 aaaagaaatt attttaatat caccatggtt agatattgct actaataatc caaagattga
     8221 aaaagttcaa aaaaaagatc cccttcttaa agcttggcaa ttacaacaag tagctcctta
     8281 ctgggcaaat ggaaaaaaga attttaaaaa tcctcaagtt agtcctcttt acagtagcca
     8341 atttaacaag atggctccaa tatcattttt tataggaacg cacgatattt tctaccccga
     8401 taatcaatta ttacatcaaa aattagcaaa agaaaacatt aaacaccact atatcgttgg
     8461 tcaaaaaatg aaccacgttt atccagtctt accgattcct gaagcagaga ctgctttcaa
     8521 acaaatgctt aaaattatca atcattaaat aaaaaggttt ccaatcaagt ttagttggaa
     8581 accttttttc tattactttt gaatgactaa ttatcttgag ataaaaactc tgagaataaa
     8641 acttcttgac taatcttttc agctaaagcc tgtttaaaag ctaatttccc taaataatag
     8701 gatttgtggt caattgttgg gagtttcaaa agttgcccaa ctaataaaca ttcctgacca
     8761 ataatataag gacatttttt cctactttct tcataacctt ttattagccc tgccgcaact
     8821 tcatcacttg tagctaatat accatctata ctaacttctt ttactaattg ataagataaa
     8881 tttagtccat cattaaagtc atgaacattc ccaactacca tatatggaga ggatagttgc
     8941 ccatagacct cttgataagc taataatgcg gattgatagg tagcagagga ttcattattc
     9001 cttgaaaata ataaaactaa gtgctccaaa cctcgtaact tcatatcaga aaatgcctct
     9061 agaaatgaag aatagcgatc aaggtaagca gaggatagat gattatattc ctgcaatttt
     9121 tcacaaacta ctattcgtcc atattttgca taagtttcaa taatgtcgag gctaattgct
     9181 cttgaagtga atattaaagc atcaatagct tccatcttga gctgtttcaa gtaggaaagt
     9241 tctaattctt ggttgtaatc cgaaggcatc acaacaagtt gatagtcggt agttttagca
     9301 gcatctaata ggccatttat taattgtgta aaataaggat ggcgcgtgtg gggaatgacg
     9361 acacctattt tttgtgtttt gcctcgactt aaatcccgcg ctagttgatt tggtatataa
     9421 tccaattcgg tgataagagc cattactttt tgtcgcgtct catcagaaac atgtgggtga
     9481 tgattgataa cacgggaaac cgttgattta gcaacacctg cctttttagc tatatcacta
     9541 atacttgtca ttataaggct cctactttaa aaatttttga atcccatatc ccactccgtc
     9601 ttcatcatta gacttagtta gttgatatgc tatcgctttg atatcatcaa aagcattatc
     9661 cataacaata gggtaaccta ccatttcaag cataggcaaa tcattatgtc catctccaaa
     9721 tgcagccgtt tcttctctag ctaactgctc tttttgaagg atgtaagcaa ttcctttaga
     9781 ttttttggct aagagatgtg taatttcaag atacgcttta cctgaacgtt gaatagtgat
     9841 ttctggtaac tccaaacttt gaagatattt ttctaattct agcatatttg cttcatcgaa
     9901 agtaatcatc ataattttaa aagtatttgt gtgaccttct aaaaactgat cctcattatg
     9961 aataaacgtt gggcattgtt gagttaaact atgttcgtat cggattcctt catctatttt
    10021 atcacaatac cagttattaa gatcgtaata agataaacta acttgaggaa aatggaaccg
    10081 tattcctctt agcaattgtt ttactgtgct ttttttaatg atttgtgtat gaataggtag
    10141 aacttgatta ttatctccta ttctataaat caatcctcca ttaaaagcaa cttggactcc
    10201 tcctaattga agtgcatcta cggcatcctt catttccata ggtgccctag cagacactaa
    10261 agttactgga atcgctgcct cacgtattaa ggtagcgtta cttttactca cttgtccttc
    10321 actatttaat agtgtaccat ccatgtcaca aaaaatacgt ttgatagtca taaaaaatac
    10381 ctctcttctt ctgaaatcag tataaatgct ggaacccgtt ccatgtcaag cttaaaatta
    10441 ctgaatcctt gcgaaaaata gattgtattt aacagatacg caatcatttt aataaatcaa
    10501 cgaactgtct gaatatgcca cttccaccat cctgttagcc taattgtacc tgattgatcc
    10561 gttcggaaaa tcttgctttt aatcttttgg aacctttgta aagtctcttg atgaggatgc
    10621 tggtaacgat tatttttacc agctgacact agaaccacac taggagacaa ctttttcaga
    10681 aaggatagac ttgatgagcc cttagaacca tgatgtcctg ctttaaggat atctactgat
    10741 aaattaggat aagcttctaa taactgcttt tctccctctt cctccaaatc accggtaaat
    10801 aagaagcctt ttcctaataa atgtccatat aaaacaattg aatcattatt ttttccatca
    10861 cccttgtgcc atgggtaaag tacttgtaaa taacttccca tgacagaaag tttatcacca
    10921 gcttctatag attttacagc tactcctatc tttgataaac gcttaacgta ctgcgaattc
    10981 gttaaagaac ctgaacttgt aatcaaacgt gcaactttaa aatgctttga gataacttcc
    11041 atatccccca tatgatctgt atcggtatga gttatcacta aattatctat cttgctaata
    11101 cctctgcttt tcaagtaagg aatgagcgtt ctctcagcat tagactggtt tactttttgt
    11161 ttccattctt caggctgttc aaatctcacc cttcccccag tatcaattaa aattgttttt
    11221 cctaaccaat ccctcactaa aatactatct ccttgtccaa catctaaaat cgcaacctcg
    11281 ttagtaattg gattcttagt gataaaaaac aaggtaaaga taataagaag ggagcaataa
    11341 cgaaaacatt tagaatgata ataatcataa agtaatccca aaattataat catgactatt
    11401 aaaagaaaaa ggctgggctt tccaaaaata agtggtcttg gaaacagttg cccagtccat
    11461 tttaataata cttctaggat ttcaaacaaa ctattaatac aacttaattt aacgaacggt
    11521 gataagacaa atataacagt taataaaggc aaaaatataa tatcaaatgc aaaagataaa
    11581 agtgctgtta atataattga tattggttga aaacccgaaa aatagtaggt tagtatggga
    11641 agaattccta cagagactgt caaagattcg atagctactt tttttatact cgaaaaatga
    11701 tcaaaggagg tcatcgtaag tataaaagca taagcaaagc taagaactcc tcccgtagtc
    11761 aaaagtgagt gggggagaga gataagacaa attaacaagc agagagcaaa gttctcatct
    11821 ttcttgacac cgctatgtga taataaagat tgaataagtg ccctaacgac tgagatacta
    11881 aagcctgtta aaccagcata aattagcgag aatggtattt gaagtaacca aacatggtct
    11941 agacgtaagc caatacgtag acagatataa cgaaaaatac cgagaaaaaa acctacttgc
    12001 atacctgaca aagcaaataa atgtataata cctagactac tatataaatc ggacatgtca
    12061 tcaaaggtct tatctagata tccaaacaaa agccctgaca tatagtgacg cataggattt
    12121 ggaaaagttt gactaataac aattgcactt ctacgccact taaacataag atcaaagaaa
    12181 gacctatgtt gaaataagcg tatctgtttt atctttgtca ctttggcgat acgataaatt
    12241 ccatgtcttt ttaaatactg acgataatca aagccattaa aatgccttct ctcctctgct
    12301 tcttcaagtt tgatatctgc aattatcatt atattattat caagattttg aaaataatct
    12361 ttctcacttt tattttttaa tgtgtaaaaa gcttgataag tgttacctga agcatctgcc
    12421 tggaatgata aaacatcacc attgatagaa atagtatcaa tacgcacttt aatctggcta
    12481 atatgattcg gtactctttg atattggaca ttcatattgt gattttgcca taaaaaataa
    12541 aataagaaaa aaattactat tgttattgtt ttaaaaatag tttcacgatc atattgataa
    12601 atcagaaaaa taaaagtaaa taaaagaaag gcacaaccaa gcattgtcca agaaaacact
    12661 agtaagtaaa tttggaagac caacaatgct aaataaatag gttttagagg aaaatactta
    12721 gtcaagtgta acatcttgac gtaatttttc tagtgttttc tcaccaatac ctgatacatt
    12781 tttgaggtca tcaatagttt taaaaccacc ttgactatca cgataatcta aaatatcttg
    12841 agctctttta gctccaacgc ctgatatggt ctgaagactt gataaatctg ctttattaag
    12901 attaattttc cccttactat ttccctgagt agcaatatta tttgctttct cctcttccac
    12961 aacagttatt ttttcacctt tttgagcaac atagataacc gtctcatctt gtaatttctg
    13021 tgctagatta acggatttac tatcagcgag attgcttagt ccacctgcac gcttaatcgc
    13081 atctgttaca cgactttgag aaggcaaact ataaacacct ggatgattga ctgccccttt
    13141 tacgtcaacc gttacttgat tagatacttt atctttgacg tgactaatca ttggtacctt
    13201 tttttctgtt atagttgata cttttttctc taaattgata tttaaatcgt cttctttatg
    13261 atgagtgcca aaatgattta aacccaaaat tccaaacaat aagagtccta caataattcc
    13321 tgttgtctcc catttatgcg atttaatttt ttccaatact atttcaaaca taaaaatatt
    13381 cctccattta cttattcgta actaaagaga ttttcctatt aaaaacaaaa aaaactattt
    13441 actaaaaagt aaatagtttc catgtaaccc ttattttaga tgtttttgtg gatcccacac
    13501 aaagcttgct aaataactaa aaattagagt taggacaagc aagacaatag ctataattgc
    13561 taaaggtaga cgataaatat aagtcaatgg atttaatttc ttaccatcat cataagtttc
    13621 attttctcta tctaaacgat caaattcttc ctgaatacga cgagatactt ctgcaatccc
    13681 ttcggcatcc atacgcttaa tatcagaaac atcaataggg ttaccaaaat tcatatcaac
    13741 acgatgcccc tttaatagat ttttaaaaac cataggacct ctatatgctg ctggcataat
    13801 tctgactttt gccatttttg caataacagc tacgcctcct ttgacatctt tggagtggcg
    13861 gcttccactc ggaaacatca ccaaagaacg gttactattt ttcagcatct ttacaggata
    13921 tcgaatagcg tcttgccctg gcttttcacg atcaataggg aaagcaccgc acattttaat
    13981 ccaccaacca aacaaacgat tcgtgaataa ttcttttttg gccataaaaa caaactgttt
    14041 gggtctagca gcaaaagcca tgtacacagg atcccaaaat gttctgtgtg gagctactaa
    14101 aatataattt tcatcatctt ttaacatttt atcttcatta tgatagtgcg cattaccatt
    14161 agccacccaa atcaagaaca taactaaagt acgtaagtaa gtatagaaca tgtttctcct
    14221 ctacctttca tcaagtaact tctattttat catgcttcct tttttttaga aagaattatc
    14281 ttaaaatcaa cctccacact ttcaaaacta gtgctaggaa gctgtaattt tgctataata
    14341 agcctatgat agatacaatt ttaaaagaaa atgaacgcat tgatcagctt ttttcaactg
    14401 atgtcaaaat catccaaaac aaagaagttt tttcctactc catagatagt gttttattgt
    14461 ctcggttccc taaacttcct tctcgtggtc ttatcgttga cttatgcagt ggtaatggtg
    14521 ctgtcggttt gtttgccagc accaaaacaa gtgcgacaat tatagagatc gaactacaag
    14581 agcgccttgc ggatatggct aagcgttcaa ttaagctcaa taaattggaa aaacaggtta
    14641 ctatgattaa tgatgattta aaaaatcttc ttgatcatgt tcaacgttca aacgttgatt
    14701 taatgctttg caatccacct tatttcaaag caagcgaaac ctctaaaaaa aatctaagcc
    14761 cgcattatct ccttgcaaga catgaaataa caacaaacct tagagagatt tgtcaaattg
    14821 ctcaacatgc tctaaaaaca aaaggacgta tcgctatggt acatcgtcca gaccgtttcc
    14881 ttgaaattat tgatacgatg cgtcagttca atttagcacc taaacgtatc caatttgttt
    14941 atccaaaatt aggaaaggat gctaatatgc ttttaattga agctattaaa gatggctcga
    15001 cagaaggtat gaaaatactt cctcctcttg ttgttcatca agataatgga gactatactg
    15061 agactatttt tgatatatat ttcggggaaa acggaaaatc tcatgattaa agttcctgct
    15121 tatatgtatg ttttagaatg ttcagatgga acactttata caggttatac tacagatgtc
    15181 aagcgccgtc tcaatacaca caataccgga aaaggtgcta aatatactcg tgcgagattg
    15241 ccagtaaaac ttctttattc tgaagctttt aatagcaaac aagaagcaat gagggcagaa
    15301 gcattattta aacaaaagac tcggaaagca aaattgactt atattaaaca acataaaaat
    15361 gaacaataat acgataatat actaagtgca atgagcctgt cctcgcttct ttaactttta
    15421 aatttcaacc tcactttaat cttttgtctt cattcttaaa gttataaggc aattttacgg
    15481 atatcactat ctacgcttta tcttaaaaaa taaaaaagga gtcgctcagc aactcctttt
    15541 tcgtaatcct cttatttatc gcctttgttg cgaataacaa atccagtttg tttttcactt
    15601 gaagtgtttt tatgaggttt tttattttct tgacgacgat tatctttctt gaacttatca
    15661 tcacgacgtt taaaatcacg acgacctcca cgatcatcac ggtcacgatt tccacgacga
    15721 tcaccacgat cattgcgtcg acgatcgcca ccacggccat tacctcgtcc acctttacct
    15781 ttaaagccac cacctgatgg tttaaatggt aatggttttt cacgtgtgat ttcaacttct
    15841 ggtaacgatt ctggatcctg aactgttaag cttaagacat aaagagcaag ttcttctggt
    15901 gtatattgag ttgctaattc taaagcatcg gctttaaatt tatcaaattt agaaactaat
    15961 tcttgatcct caaagtcacg tgcaatacgt ttcaatgcaa cttttttctt agcctgaaat
    16021 gcttcgcttg cagtagcagg cttcattcca gtcatacgtt ttttagttaa gttttcaata
    16081 attgtcaaat atcccatttc gtttggtgaa acaaaagtga tagattgacc agatttacca
    16141 gcacgaccag tacgtccaat acggtgaaca taactttctg gatcttgggg aatatcatag
    16201 ttgtaaacat gtgtaacgcc tgaaatatct aaaccacgcg cagcaacatc tgtggcaaca
    16261 agaatatcaa tatggtcatt tttaaagtct ctgataacac gaagacgttt attttgatct
    16321 aagtctccat gaattccttc tgctcggaaa ccacgtaatt ttagaccacg agttaattca
    16381 tctacacgac gtttcgtacg tccaaagaca attgacaatt ctggttgatc aacgtccatc
    16441 aaacgtgtca tagtatcgaa tttttcattt tccttaacgc gaacataata ctgatcaaca
    16501 ttcacattag ttaattcagt tgctttaatt ttaacatgct caggatcttt catgaattta
    16561 accccgatac gtttgattgg atcaggcatt gtcgctgaga ataaaagagt ttgacgtgct
    16621 tctggtacac gactaatgat tgcttcaatg tcctctaaaa atcccatgtt aagcatttcg
    16681 tctgcttcat caagaataag agtttcgatg tgattaagtt taagtgcttt acgtttaatt
    16741 aaatcaagta atcgacctgg agttccaaca acaacgtgtg ctcctgaacg taatgccttg
    16801 atttgttttt caatacttga cccaccataa acagagcgga ccttaacacc tttatcgcgt
    16861 ccaaaacgga acaattcttc ttgtgattgt actgcaagtt cacgtgtagg agcaataata
    16921 agtgcttgaa tagtattatc ttcagtatga attttattta aagttggtaa cccaaaagct
    16981 gcagtttttc ctgtacctgt ctgcgcttga ccaatgacat ctttgccttc aagagcaagt
    17041 gggatagtca tttcttggat tggtgatggc tctacaaagc ctgctttttc taccgctgaa
    17101 agaatatctt gcgataagtt aagttctgta aatttcaaat gtttctcttt tctaaaagac
    17161 agtgcgaagc tgccttatga acctttatta gttatctttt ctgacaacct acttagtgta
    17221 acacaattaa aagtaaaaag atataaaata aggtcaatct aataaataac acgctttcat
    17281 aaattgctag ttctattagt tgcttcagaa aatagaaaaa aggcaataaa aaccctccat
    17341 ctataaagaa ggagggttca tagtttatct atctagaacg caacgtctgc ttcacttgga
    17401 atctccttag tgatatagtg acgaatctct ttacttgtta gtacttcttt aagtactttt
    17461 attttatcac tatttttatt atcttcacga gctaccaagg cacctgcaaa acggatatct
    17521 ggttttttct catataataa tctatccctt ttaggaacta gattgatttt tgtcacatat
    17581 ccagggaaat taaagacaag gtcatattca aaataggcct gattgaggtt aagtaatgct
    17641 acaggttcaa ttcgtaattt tttagggtta gtaataatat cttcgatagc cttggtcttt
    17701 ttggacgtat tctttaaggt gataagtttc ttttcttgca ataatagcag agctctagtc
    17761 atattggcag gatcagacgg aatcgctacc ttagcaccgt ctttaagctc ggcaatattt
    17821 ttcaaatgtt gaccataaaa accagccaat gaatgataaa ttggtgtaat tgacactaga
    17881 tgaccatcat tttccttatt aaagatactc atgaaaaatt catgttgtaa aaggttagca
    17941 tcatgttctt tgttttctaa agcaatgtta gcctgaatat agtcagagac catgacaacc
    18001 tttaaagtat atcctttttc ttttacatta tctttaatca aatctaaaaa ggtagatgta
    18061 ggtttgctgt aagttgcaac agtaatcacc ttttctttac tattcttatc tttaatttgc
    18121 ttagacgaca ttgcttgctt agacgacatt gcttgcttag tacatgccac taaacataaa
    18181 gacatcatga ttagacatgt tagggaaagt aattttttca tagttcctct tttcttttat
    18241 tttcttgagt agcggtttgc tataaaatag cccaaactct gtaagataaa cacatatatt
    18301 ataaataaga caacaatcaa gtacataact gggtagtcat actcttgata gccaaaacga
    18361 taggcatatt cacccaaacc tcctgcacca ataactccca ttattgtcga gtagccaaga
    18421 atacttatag ccgttgcagt aaatgataaa acaaggtcaa tcttgatgga ggggattaaa
    18481 aaataataaa ttatctgtct cttgttagcc cctagactga gagctcgatc tacaacaact
    18541 tgaggtatat ttaataaagc ttgttccaca taacgtgcat acaaactgac tgaaaccaaa
    18601 gtaagaggta agattgccgc tatcgttcca aatgaggtcc caaaaattaa tcgattgaga
    18661 gggattaaga taaaaataaa aataaggaaa gggacactcc gtaacgtacc taaaaataaa
    18721 tttagtagtt gataggctaa agaatgttta atcaagtaag actttctcaa tgaaaacaat
    18781 aaaatacctg ttggaaaagc aattagaaaa caaagaatca aggttaaccc taacattaaa
    18841 ttggtttccc aaaatgattt taagatacct tcgccatgtt tttcaataat gtcaatcatg
    18901 taacaaaaac tcctttactt gatctacgta gtttgacttt aattgattca gagttgcttt
    18961 cactggaaca acagtatcta caatagtctg gtgatccaaa atcattacac gatcacaata
    19021 ttgttttaaa agagataggt gatgagcaat catcaagata gagataccat atttttcacg
    19081 aagatcagac aacaagttaa atatctcttt ttcactatta gtatctaagg cagagctgat
    19141 ctcatcacat agaataagtt tcggtttttg caaaacagcc atagcgattg caactttttg
    19201 ttgctgccca tcactcaaac tatggcattt cacctgtttt aaatccatca aacctaaaaa
    19261 atctaag
//
LOCUS       DATRUK010000037        19038 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.037, whole genome shotgun sequence.
ACCESSION   DATRUK010000037 DATRUK010000000
VERSION     DATRUK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19038
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.037"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..97)
                     /locus_tag="VBE34_001260"
     CDS             complement(<1..97)
                     /locus_tag="VBE34_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7263980.1"
                     /translation="MTKHKHLTLSDRNDIQSGLDRGETFKAIGLNL"
     gene            300..2339
                     /locus_tag="VBE34_001261"
     CDS             300..2339
                     /locus_tag="VBE34_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7263981.1"
                     /translation="MKRKYFVLNTVTVLTLATAMNTSSSYANSTETSASVAPTTNTIV
                     QTNDSNPTAKFASESGQSVIGQVKPANSAALTTVDTPHISAPDALKTTQSSPVVESPS
                     TKLTEETYKQKDGQDLANMVRSGQVTSEELVNMAYDIIAKENPSLNAVITTRRQEAIE
                     EARKLKDTNQPFLGVPLLVKGLGHSIKGGETNNGLIYADGKISTFDSSYVKKYKDLGF
                     IILGQTNFPEYGWRNITDSKLYGPTHNPWNLAHNAGGSSGGSAAAIASGMTPIASGSD
                     AGGSIRIPSSWTGLVGLKPTRGLVSNEKPDSYSTAVHFPLTKSSRDAETLLTYLKKSD
                     QTLVSVNDLKSLPIAYTLKSPMGTEVSQDAKNAIMDNVTFLRKQGFKVTEIDLPIDGR
                     ALMRDYSTLAIGMGGAFSTIEKDLKKHGFTKEDVDPITWAVHVIYQNSDKAELKKSIV
                     EAQKHMDDYRKAMEKLHKQFPIFLSPTTASLAPLNTDPYVTEKDKRAIYNMENLSQEE
                     RIALFNRQWEPMLRRTPFTPIANMTGLPAISIPTYLSESGLPIGTMLMAGANYDMVLI
                     KFATFFEKHHGFNVKWQRIIDKEVKPSADLIQPTNSLFKAHSSLVNLEENSQVTQVSI
                     SKKWMKSSVKNKPSVMAYQKALPKTGDTESSLSPVLVVTLLLACFSFVTKKNQKS"
     gene            2449..3027
                     /locus_tag="VBE34_001262"
     CDS             2449..3027
                     /locus_tag="VBE34_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7263982.1"
                     /translation="MKVKNKILTMVALTVLTCATYSSIGYADTSDKNTDTSVVTTTLS
                     EEKRSDELDQSSTGSSSENESSSSSEPETNPSTNPPTTEPSQPSPSEENKPDGSTKTE
                     IGNNKDISSGTKVLISEDSIKNFSKASSDQEEVDRDESSSSKANDEKKGHSKPKKELP
                     KTGDSHSDTVIASTGGIILLSLSLYNKKMKLY"
     gene            complement(3061..4302)
                     /locus_tag="VBE34_001263"
     CDS             complement(3061..4302)
                     /locus_tag="VBE34_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEO7263983.1"
                     /translation="MVLQDFDNLLKKYAQLIISKGLNVQKGHTLALTIDVEQVHLARL
                     LTEVAYEKGASEVIVDYTDDFITRQRLLHASDEVLTNVPQYTVDKSLALLNKKASRLV
                     VKSSNPNAFATVDPKRLSKTTRATAIALEEQSRAIQANKVSWNVAAAAGREWAALVFP
                     ELKTSDQQVDALWDTIFKLNRIYEDNPIAAWDAHEAKLLEKATRLNQEQFDALHYTAP
                     GTDLTLGMPKNHIWEAAGSLNAQGETFIANMPTEEIFSAPDYRRADGYVTSTKPLSYA
                     GVIIENMTFTFKDGKIINVTAEKGQETIQRLIEENDGARSLGEVALVPHKTPISLSGL
                     IFFNTLFDENASNHLAIGSAYAFNVEGGTEMTSQELDEAGLNRSSTHVDFMIGSEQMD
                     IDGIRADGTAVPIFRNGEWAI"
     gene            4480..4608
                     /locus_tag="VBE34_001264"
     CDS             4480..4608
                     /locus_tag="VBE34_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEO7263984.1"
                     /translation="MAFGDNGQRKKTGFEKLTLFVVILMVLVTVGGLVFGAISAIM"
     gene            4669..5982
                     /gene="obgE"
                     /locus_tag="VBE34_001265"
     CDS             4669..5982
                     /gene="obgE"
                     /locus_tag="VBE34_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEO7263985.1"
                     /translation="MSMFLDTAKISVKAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFKVNEGLRTLMDFRYNRNFKAKAGEKGMTKGMHGRGAEDLIVSLPPGTTVRDANT
                     GKVITDLVEHDQEFVVARGGRGGRGNIRFATPRNPAPEIAENGEPGEERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVVSAAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYDDYISINNELETYNLRLME
                     RPQIIVANKMDMPDSEENLAAFKEKLAANYDEFDDMPMIFPISSLAHQGLENLMDATA
                     KLLANTEEFLLYDETDMQEDEAYYGFNEDERPFEITRDDDATWVLYGDKLEKLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDIVRIGNFEFEFVD"
     gene            6008..6166
                     /locus_tag="VBE34_001266"
     CDS             6008..6166
                     /locus_tag="VBE34_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263986.1"
                     /translation="MGDKPISFRDKDGNFVSAADVWNAEKLEELFNTLNPNRKLRLER
                     EKLAKEKG"
     gene            complement(6259..6609)
                     /locus_tag="VBE34_001267"
     CDS             complement(6259..6609)
                     /locus_tag="VBE34_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7263987.1"
                     /translation="MKDYINRILHFIKEHMTYHVNFIDDFLDIKWEKVSNIHLRFWTT
                     IIAYLVIFILSISTVILNLVLLFQGFLTQNPIIYLLFFITLVCAFYFAYKFITYTPTI
                     VKNALQYIKKLKNV"
     gene            complement(6759..7499)
                     /locus_tag="VBE34_001268"
     CDS             complement(6759..7499)
                     /locus_tag="VBE34_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7263988.1"
                     /translation="MAELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLESITSGKVVVDGFELSNPKTDIDKARENIGMVFQHFNLFPHMSVLENI
                     TFAPIELGKESKEAAEKHGMELLEKVGLADKANAKPDSLSGGQKQRVAIARSLAMNPD
                     ILLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGRF
                     LEDGTPEQIFDTPQHPRLQDFLNKVLNV"
     gene            complement(7499..9682)
                     /locus_tag="VBE34_001269"
     CDS             complement(7499..9682)
                     /locus_tag="VBE34_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO7263989.1"
                     /translation="MKHKLKAFTLALLSIFFVFGGKVSAETVNIVSDTAYAPFEFKDS
                     DQTYKGIDVDIVNEVAKRAGWNVNMTYPGFDAAVNAVQSGQADALMAGTTVTEARKKV
                     FNFSDTYYDTSVILYTKNNNKVTNYKQLKGKVVGVKNGTAAQSFLEENKSKYGYKVKT
                     FDTSDLMNNSLDSGSIYAAMDDQPVVQFAINQGKAYAINMEGEAVGSFAFAVKKGSGH
                     DNLIKEFNTAFAQMKSDGTYNDIMDKWLGKDATKTSGKATGNANEKATPVKPSYKIVS
                     DSSFAPFEYQNGKGKYTGFDMELITKIAKQQGFKLDISNPGFDAALNAVQSGQADGVI
                     AGATITEARQKIFDFSDPYYTSSVILAVKKGSNVKSYQDLKGKTVGAKNGTASYTWLS
                     DHADKYNYHVKAFDEASTMYDSMNSGSIDALMDDEAVLAYAINQGRKFETPIKGEKSG
                     DIGFAVKKGANPELIKMFNNGLASLKKSGEYDKLVKKYLSTASTSSNDKAAKPVDEST
                     ILGLISNNYKQLLSGIGTTLSLTLISFAIAMVIGIIFGMMSVSPSNTLRTISMIFVDI
                     VRGIPLMIVAAFIFWGIPNLIESITGHQSPINDFVAATIALSLNGGAYIAEIVRGGIE
                     AVPSGQMEASRSLGISYGKTMQKVILPQAVRLMLPNFINQFVISLKDTTIVSAIGLVE
                     LFQTGKIIIARNYQSFRMYAILAIMYLVIITLLTRLAKRLEKRLK"
     gene            9865..10785
                     /locus_tag="VBE34_001270"
     CDS             9865..10785
                     /locus_tag="VBE34_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7263990.1"
                     /translation="MNKRILNSQFAVYKNWPSWLLLLFACFMVYLFFFVGLTLGGVGI
                     VLVSSVLYKFSSYTFFEFINLFNNIYGELGTFSFSAFLLLIWVKFVEKRPFSELGFST
                     KFKRTLWSLIKGWSIGFILFSISVITAYILGGLDFHSYDVSKATIFYVVTLLPFWLIQ
                     SGTEELLTRGWLLPLINHRFHLAVAIGVSSTLFGILHLVNAHVTFLSIVSIICSGVLM
                     SLYMIKSGNIWSVAALHGAWNFSQGNLYGIAVSGQKAGASLLHFTVKENAPDWISGGA
                     FGIEGSLISIFVLLAAIIYLLWLIKTEETD"
     gene            10846..12837
                     /gene="uvrB"
                     /locus_tag="VBE34_001271"
     CDS             10846..12837
                     /gene="uvrB"
                     /locus_tag="VBE34_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEO7263991.1"
                     /translation="MIDRKDTNRFKLVSKYSPSGDQPQAIETLVDNIEGGEKAQILKG
                     ATGTGKTYTMSQVIAQVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGQEISRDQLLNNLVDIQFERNDIDFQRGKFRVRGDVVEFFPASRDEHAFR
                     IEFFGDEIDRIREIESLTGRVLGEVEHLAIFPATHFMTNDEHMEEAISKIQAEMENQV
                     ELFEKEGKLIEAQRIRQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPFTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYEMEQTDTVVEQIIRPTGLLDPEVEVRPSMGQMDDLLGEINLRTEKG
                     ERTFITTLTKRMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSNGHVIMYADKITDSMQ
                     RAMDETARRRRLQMDYNEKHGIVPQTIKKEIRDLIAITKSNDSDKPEKVVDYSSLSKK
                     ERQAKIKALQKQMQDAAELLDFELAAQIRDVILELKAID"
     gene            13080..18404
                     /locus_tag="VBE34_001272"
                     /pseudo
     CDS             13080..18404
                     /locus_tag="VBE34_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            18556..>19038
                     /locus_tag="VBE34_001273"
     CDS             18556..>19038
                     /locus_tag="VBE34_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO7263992.1"
                     /translation="MSRSQKVILTNMCLIEDNKGRIVMQIRDPKRYSWSGAALPGGHI
                     EEGESLYDALVREVREETGLTISNPKLVGMKHWYTDEGIRYLVFLYKTYDFSGELQSS
                     DEGDVIWIERSQLDKIELAYDMLNLLRVFDEDDISEMFYSKRLEDGFIRKILLNKKEL
                     G"
BASE COUNT         6388 a         3101 c         3673 g         5876 t
ORIGIN      
        1 gtagattaag cccgatggct ttaaaggttt ctcctctatc caaaccagat tggatgtcat
       61 tacggtctga gagagttagg tgtttgtgtt ttgtcatgat ggataaactc atttctaacc
      121 caaacgtctc agacttaatt ccaccataaa aatccttttc acactaactt ccggttttgg
      181 agggcagacg gaacttactt tgagaaatta cttaatataa aataacagtt aatattttag
      241 caatttgata acacttagat tataatgtaa acgtatactt ttttagattg gaagatgaaa
      301 tgaaacgtaa atactttgtt cttaatacgg tgacggtttt aacgttagct actgcaatga
      361 atactagcag tagctatgct aatagtactg agacaagtgc ttcagtagct cctactacaa
      421 atactatcgt tcaaactaat gacagtaatc ctaccgcaaa atttgcatca gaatcaggac
      481 aatctgtaat aggtcaagta aaaccagcta attctgcggc gcttacaaca gttgacacgc
      541 ctcatatttc agctccagat gctttaaaaa caactcaatc aagtcctgtc gttgagagtc
      601 cttctactaa gttaactgaa gagacataca aacaaaaaga tggtcaagat ttagccaaca
      661 tggtgagaag tggtcaagtt actagtgagg aactcgtcaa tatggcatac gatattatcg
      721 ctaaagaaaa cccatcttta aatgcagtca ttactactag acgccaagaa gccattgaag
      781 aggctagaaa acttaaagat actaatcagc cgtttttagg tgttcccttg ttagtcaagg
      841 ggttagggca cagtattaaa ggtggtgaaa ccaataatgg cttgatctat gcagatggaa
      901 aaattagcac atttgacagt agctatgtca aaaaatataa agatttagga tttattattt
      961 taggacaaac gaattttcca gagtatgggt ggcgtaatat aacagactct aaattatacg
     1021 gtccaacgca taatccttgg aatcttgctc ataacgctgg tggctcttct ggtggaagtg
     1081 cagcagctat tgctagcgga atgacgccaa ttgctagcgg cagtgatgct ggtggttcta
     1141 tccgtattcc atcttcttgg acgggcttag taggtttaaa accaacaaga ggattggtga
     1201 gtaatgaaaa gccagattcg tatagtacag cagttcattt tccattaact aagtcatcta
     1261 gagacgcaga aacattgtta acttacctaa agaaaagcga tcaaacgcta gtatcagtta
     1321 atgatttaaa atctttacca attgcttata ctttgaaatc accaatggga acagaagtta
     1381 gtcaagatgc taaaaatgct attatggaca acgtcacatt cttaagaaaa caaggattca
     1441 aagtgacaga gatagattta ccaattgatg gtagagcatt aatgcgtgat tattcaacct
     1501 tggctattgg catgggagga gctttttcaa caattgaaaa agacttaaaa aaacatggtt
     1561 ttactaaaga agacgttgat cccattactt gggcagttca tgttatttat caaaattcag
     1621 ataaggctga acttaagaaa tctattgtgg aagcccaaaa acatatggat gattatcgta
     1681 aggcaatgga gaagcttcac aagcaatttc ctattttctt atcgccaacg accgcaagtt
     1741 tagcccctct aaatacagat ccatatgtaa cagagaaaga taaaagagcg atttataata
     1801 tggaaaactt gagccaagaa gaaagaattg ctctctttaa tcgccagtgg gagcctatgt
     1861 tgcgtagaac accttttaca ccaattgcta atatgacagg actcccagct atcagtatcc
     1921 cgacttactt atctgagtct ggtttaccca tagggacgat gttaatggca ggtgcaaact
     1981 atgatatggt attaattaaa tttgcaactt tctttgaaaa acatcatggt tttaatgtta
     2041 aatggcaaag aataatagat aaagaagtga aaccatctgc tgacctaata cagcctacta
     2101 actccctctt taaagctcat tcatcattag taaatttaga agaaaattca caagttactc
     2161 aagtatctat ctctaaaaaa tggatgaaat cgtctgttaa aaataaacca tccgtaatgg
     2221 catatcaaaa agcacttcct aaaacaggtg atacagaatc aagtctatct ccagttttag
     2281 tagtaaccct tttattagct tgttttagct ttgtaacaaa aaagaatcag aaaagttgat
     2341 tgtaatgtaa aataattcct atttttcact ttttagtatc atattttcga tttttatgtt
     2401 gtaataaatt ataatgaaga tgtaaggaat tataaaatag gaaataatat gaaagtgaaa
     2461 aataagattt taacgatggt agcacttact gtcttaacat gtgctactta ttcatcaatc
     2521 ggttatgctg atacaagtga taagaatact gacacgagtg tcgtgactac gaccttatct
     2581 gaggagaaaa gatcagatga actagaccag tctagtactg gttcttcttc tgaaaatgaa
     2641 tcaagttcat caagtgaacc agaaacaaat ccctcaacta atccacctac aacagaacca
     2701 tcgcaaccct cacctagtga agagaacaag cctgatggga gcacgaagac agaaattggc
     2761 aataataagg atatttctag tggaacaaaa gtattaattt cagaagatag tattaagaat
     2821 tttagtaaag caagtagtga tcaagaagaa gtggatcgcg atgaatcatc atcttcaaaa
     2881 gcaaatgatg agaaaaaagg ccacagtaag cctaaaaagg aacttcctaa aacaggagat
     2941 agccactcag atactgtaat agcatctacg ggagggatta ttctgttatc attaagtctt
     3001 tacaataaga aaatgaaact ttattaaata taaaaaggag gactaacctc ctttttatat
     3061 ttaaatagcc cattcgccat ttctaaagat tgggacagca gttccatctg cacgaatacc
     3121 atcaatatcc atttgttctg aaccaatcat aaaatcaaca tgtgttgaag aacgatttaa
     3181 accagcttca tccaattctt gacttgtcat ttctgttcct ccttctacat tgaaggcata
     3241 tgcagatcca atagcgaggt gattagaggc attttcatcg aataaagtat taaagaaaat
     3301 tagtccagac agtgaaattg gtgttttatg tgggacaagt gcaacttccc caagcgatct
     3361 tgccccatca ttttcctcga tcaagcgttg gattgtttct tgcccttttt ctgcagtgac
     3421 attgataatt ttaccgtctt taaaagtaaa cgtcatattt tcgataataa cgccagcata
     3481 actgagaggt tttgtacttg tcacataccc atctgcacga cggtaatcag gtgctgaaaa
     3541 gatttcttca gtaggcatat tagcgataaa agtctctccc tgagcgttga gactaccggc
     3601 tgcctcccaa atgtgatttt taggcatacc aagcgttaaa tctgtacctg gtgcggtata
     3661 atgaagagca tcaaattgtt cttgatttag tctagtagct ttttctaata atttagcttc
     3721 atgtgcatcc caagcagcaa taggattatc ttcataaata cgatttaatt tgaaaatggt
     3781 atcccaaaga gcatcaactt gttggtcgct tgtttttaat tctgggaaga caagtgcagc
     3841 ccattctcta ccagcagctg cagccacgtt ccaagatact ttattagctt gtattgctct
     3901 actttgttcc tctaaggcaa tagcggttgc tctagttgtt ttagataaac gtttaggatc
     3961 aacagtagcg aaagcgttag ggttagaaga tttcacaact aatcgactag ccttcttatt
     4021 taatagcgct aaagatttat caacggtata ctgtggaaca ttcgtgagaa cttcgtctga
     4081 agcatgaagt agtcgctggc gcgtgataaa atcatctgta taatcaacaa taacttcact
     4141 tgctcccttt tcataagcga cttcagttaa aagccttgct aagtggactt gttccacatc
     4201 gattgttaaa gcgagagtgt gccctttttg gacatttaaa cctttagaaa taattaattg
     4261 ggcatatttt tttaaaaggt tgtcgaaatc ttgtaaaacc atactagctc ctttaaattt
     4321 actatttttt ataatttatc ataaactctt ataaagcaca aggaaatatg gaagcgattt
     4381 aataaagata tatttaagct aacggtttgt agtttgcgaa ataagacgtt tcgtgttaat
     4441 atagacttaa ctatatattc agtaaatgag gtgacatgag tggcttttgg agataacgga
     4501 caacgaaaaa aaactgggtt tgaaaaacta actctttttg ttgttatttt gatggtacta
     4561 gtaacagttg gtggtttggt atttggtgct attagtgcaa tcatgtaatg agaaacgctg
     4621 taaagcgttt ttttatactg aatagaataa agaataggaa ttttagaaat gagtatgttt
     4681 ttagatactg ccaaaattag tgttaaggca ggtcgcggtg gtgatggcat ggttgctttt
     4741 cgccgtgaga aatatgtccc caatggaggc ccgtggggcg gtgatggcgg taaaggcgga
     4801 tcggtcattt ttaaagtaaa cgaaggttta cgaactttga tggattttcg atataaccgt
     4861 aattttaaag caaaagctgg tgaaaaaggg atgactaaag gtatgcacgg tcgaggtgca
     4921 gaagacctta ttgtgtcatt accacctgga acaactgttc gtgatgctaa tacaggtaag
     4981 gttattaccg accttgtgga gcatgatcag gaattcgttg tagccagagg tggccgcggt
     5041 ggtcgtggaa atattcgctt tgcaacccca cgtaatccag ctcctgaaat tgctgaaaat
     5101 ggggagcctg gcgaagaacg tgaattacaa ttagaattaa aaattttggc tgatgttggt
     5161 ctcgttggtt tcccatcagt tgggaagtcg acgctactta gtgttgtttc agcagcaaag
     5221 cctaaaattg gtgcctatca ttttacgaca attgtcccta atttagggat ggttcgtaca
     5281 aaatcaggtg atagctttgc aatggctgat ctccctggtt tgattgaagg ggctagtcaa
     5341 ggtgtcggat taggaactca gttccttaga catattgagc gtacacgtgt tattctacat
     5401 gttattgata tgtcggcaag tgaaggacga gacccttacg atgactatat ttcaattaat
     5461 aatgagttgg aaacttacaa tcttcgattg atggaacgtc cgcaaattat tgttgccaat
     5521 aaaatggata tgcctgattc agaagagaat ttggcagcat tcaaagaaaa attagctgca
     5581 aactatgatg agtttgatga catgcctatg atttttccaa tttcaagttt agcacatcaa
     5641 ggactagaga accttatgga tgcgacggct aaattattag ccaatacaga agagtttctt
     5701 ctttatgacg aaactgacat gcaagaagat gaagcatact atggatttaa cgaagatgaa
     5761 cgtccatttg agattacacg tgatgatgat gcaacgtggg tattgtatgg tgataagcta
     5821 gaaaaactct ttgttatgac aaatatggaa cgcgatgaat caataatgaa atttgctcgt
     5881 cagttacgtg gtatgggcgt tgatgaagca cttcgagagc gcggagctaa ggatggagat
     5941 attgttcgta taggtaactt cgaatttgaa tttgtagatt aatgcagtac ttgattggag
     6001 gttttttatg ggagataaac cgatatcatt tcgtgataaa gatgggaact ttgtttcagc
     6061 ggccgatgtc tggaatgctg aaaaattaga agaactcttt aacacattaa atcctaatcg
     6121 taaacttcgt ttagaacgcg aaaaattagc aaaagaaaaa ggatagttaa gtaactatcc
     6181 tttttagtta ttttgggtat gaaaaaagac tctaaaacat aattgataga gtgttaaggt
     6241 acagattact ttattatttt aaacgttttt gagctttttg atatactgaa gagcattttt
     6301 tacaatggtt ggagtataag taataaattt ataagcaaaa taaaaagcac aaactaaagt
     6361 tataaaaaat agtaagtaaa taatagggtt ttgcgttaag aatccttgaa aaagaagtac
     6421 aaggttaaga attacggtag agatacttaa gataaaaata actagataag caataatagt
     6481 tgtccaaaac cttaaatgga tgttactcac cttttcccat ttaatatcta aaaagtcatc
     6541 tataaagttg acatgataag tcatatgttc tttaataaag tgcaatattc tgttaatata
     6601 gtctttcata ctgctatatt atcatactga aagcccttgt gcaacattat tcaaacattc
     6661 tagctgatgt tttgctaatt ttagaagaaa acttatccta ttataatttg tttgtgatca
     6721 ggatataaaa aaagatgaca cgaagtcatc tttttttatt aaacgtttaa aactttatta
     6781 aggaagtctt ggagacgtgg gtgttgaggt gtatcaaaga tttgctcagg tgtcccatct
     6841 tcgaggaaac gaccaccatc tgtaaagata acacgattgg caacttgacg agcaaatccc
     6901 atttcgtgtg tcacaattaa catagtcata ccttgttcag ctaaatcttt cataacattg
     6961 agaacatctc caaccatttc tggatcaagt gcagaagttg gttcgtcaaa aagtaagata
     7021 tctggattca tcgctaagct tcttgcgata gctacacgtt gtttttgtcc tccagataaa
     7081 ctatctggtt tggcattagc tttatcggct aaaccaactt tttcaagtaa ttccatccca
     7141 tgcttttcag ctgcttcttt agattcttta ccaagttcta taggagcaaa agtgatgttt
     7201 tcaagaactg acatatgtgg aaaaagattg aagtgttgaa ataccattcc gatattttca
     7261 cgtgctttgt ctatgtctgt ttttggattt gataactcaa agccgtccac aacaactttt
     7321 ccacttgtga ttgattcaag taagttcaat gtgcgaagga aagttgattt tcctgaacca
     7381 gaaggtccaa taatacaaac aacatcacct tcataaaatt tagcatcaat accttttaaa
     7441 acttcatttt gcccatacga cttgtgtaaa tcttggacat caatttttaa ttctgccatt
     7501 atttaagcct cttttctaaa cgttttgcta gacgggtgag aagggtaatg attacaagat
     7561 acataattgc aaggatagca tacatacgga atgattgata gttacgtgca attatgattt
     7621 taccagtttg gaagagttcc acaagtccga ttgctgatac aattgttgta tcctttaatg
     7681 agatgacaaa ttggttgata aagtttggta acataaggcg tactgcttga ggtaagataa
     7741 ccttttgcat agttttgccg taagaaatac ctaagctgcg acttgcttcc atttgaccag
     7801 aaggaacagc ttcaatacca ccacgtacaa tttcagcaat gtacgcacca ccatttaaag
     7861 aaagagcgat agtagcagca acgaagtcat taattggact ttggtgacct gtgatgcttt
     7921 cgattaaatt aggaataccc cagaaaataa aagcggccac aatcatgagt ggaataccac
     7981 ggacaatatc aacaaaaatc attgaaattg tgcggagagt attacttggt gatacgctca
     8041 tcataccaaa gataatacca ataaccatag caatcgcaaa cgagataaga gttaaactta
     8101 aagtagttcc aataccagat agcaattgtt tgtagttatt agaaattaac cctaaaatag
     8161 ttgattcatc tacaggttta gcagctttat cgtttgaaga agtgctggct gtggaaaggt
     8221 attttttaac aagtttatcg tactcacccg attttttgag tgaagcaaga ccgttgttaa
     8281 acattttaat taattctgga tttgcccctt ttttcactgc aaatccgata tcgcctgatt
     8341 tttcaccttt gataggtgtt tcaaatttac gaccttgatt aatagcgtaa gcaagaacgg
     8401 cttcgtcatc cattagagca tcaattgaac ctgagttcat actatcatac attgtagatg
     8461 cttcatcaaa tgctttaaca tgatagttgt acttatctgc gtggtctgat aaccaagtat
     8521 atgaggcagt accattttta gcaccaactg tttttccttt taaatcttgg tatgatttga
     8581 cattgcttcc ttttttaacc gctaagataa cgctagatgt gtaataagga tcagaaaaat
     8641 caaagatttt ttggcgtgct tctgtgattg tggctcctgc aataacaccg tcagcttgcc
     8701 cagattggac agcatttaaa gcggcatcaa aacctggatt tgagatatca agtttaaaac
     8761 cttgctgttt agcaattttc gtgattaatt ccatatcaaa accagtatat ttccctttac
     8821 cgttttgata ttcgaatggt gcgaatgaag aatcagaaac aattttataa cttggcttta
     8881 caggagttgc tttttcattg gcattacctg ttgctttgcc gcttgttttt gtagcgtctt
     8941 ttccaagcca tttatccatg atgtcattat aagtgccatc tgatttcatt tgtgcaaaag
     9001 ctgtgttaaa ttctttaatt agattatcgt gtccactacc ttttttgaca gcaaatgcaa
     9061 agctaccaac tgcttcgcct tccatgttaa tggcgtaagc ttttccttga tttatcgcaa
     9121 attgcacaac tggttgatcg tccatagcgg cgtaaataga accagaatca aggctgttat
     9181 tcattaggtc gcttgtatca aatgttttaa ctttatagcc gtatttagat ttattttctt
     9241 ctaagaagct ttgagcagct gttccatttt ttacaccgac tacttttcct tttagttgtt
     9301 tgtagtttgt gactttatta ttatttttag tataaagaat aacggaagta tcgtaataag
     9361 tatctgagaa attaaagact tttttacgtg cttcagtaac agtagttccg gccattagcg
     9421 catctgcctg tccagattga acagcgttaa ctgcggcatc aaaacctgga tacgtcatgt
     9481 taacattcca gccagcacgc ttagcgactt cgttaacgat gtcaacatcg attcctttat
     9541 aagtttgatc agaatcttta aattcgaatg gagcgtatgc tgtatcagaa acaatattca
     9601 cagtctctgc actgaccttt ccaccaaaca caaagaatat tgagagtaaa gcaagcgtaa
     9661 aagcttttaa cttgtgcttc atgtaaatct cctttttata aatatgtgtt ttattatatc
     9721 aaaataaata agatagagca acaaaatgtc atttctatgt tagaaaacgt aacatgtatg
     9781 ttatgttaat tttatatata tctttagtta gtattatgat ataattgttc tagtgtcatc
     9841 acacaaaaag gatttaggat tattatgaat aaaaggattt taaatagtca gttcgctgtc
     9901 tataaaaact ggccgtcatg gctattattg ttatttgctt gttttatggt atacctattt
     9961 tttttcgtcg gtctaactct aggtggtgtg ggaattgttc tagtgtcttc tgtactctat
    10021 aagttttctt cctatacttt ttttgagttt attaatttat ttaataatat ctatggagaa
    10081 ttaggaacct ttagtttttc agcattcctt ttgcttatct gggtaaaatt cgtagaaaaa
    10141 agaccttttt cagaattggg tttttctaca aagtttaaaa gaactctgtg gtcacttata
    10201 aaaggctgga gtataggttt tattctattt tcaatttctg tcatcactgc ttatatattg
    10261 ggaggacttg actttcatag ctatgatgtt tctaaggcaa cgattttcta tgttgtaact
    10321 ctccttcctt tttggcttat tcaaagcggt acggaagaat tgctaacacg agggtggctt
    10381 cttcctctaa ttaatcatcg ttttcattta gctgttgcca taggggtctc atccacttta
    10441 tttggcattt tgcacttggt aaatgcccat gtcacttttc tttctattgt aagtattata
    10501 tgttcgggag ttttaatgtc cttgtatatg attaaaagtg gtaatatttg gagcgttgca
    10561 gccttacatg gagcttggaa ttttagccaa ggtaatctct atggtattgc agtaagtggt
    10621 cagaaagcag gagcatctct tttgcatttt accgttaaag aaaacgcacc ggactggata
    10681 tcaggtggtg cattcggaat agaaggaagt ttgatttcta tttttgttct cttagctgca
    10741 attatatacc ttctatggct aattaagact gaagaaacag attgagtgtt gcagctcagt
    10801 ctttttcttt tttctatgtt aaaataaagt aatcgaggta aagaaatgat agatagaaaa
    10861 gatactaacc gatttaaatt agtttccaaa tatagtcctt caggagatca acctcaagct
    10921 attgaaacgt tggttgacaa tattgaagga ggagaaaaag ctcagatttt aaaaggtgct
    10981 actggtactg gtaaaaccta cacaatgagc caagttatcg cccaagttaa caagccaacc
    11041 ttggtgattg cacataataa aaccctagca ggtcaacttt atggagagtt taaagaattt
    11101 tttcctgaca atgccgtaga gtactttgtt tcatattatg attattatca accagaagct
    11161 tatgtgcctt cttcggatac ttatattgaa aaagatagct cagtcaatga cgaaattgac
    11221 aaattacgtc actcagcgac ttcttcgtta ttagaaagaa atgacgttat tgttgtagca
    11281 tctgtttctt gtatttacgg tttaggttct cctaaagagt atgcagacag tgttgttagc
    11341 cttaggccag gtcaagaaat ttcacgtgat cagttattga acaatctcgt tgatattcaa
    11401 tttgagcgta acgatattga ttttcaacgt ggaaaatttc gcgttcgtgg cgatgttgta
    11461 gaattttttc ctgcgagtcg tgatgagcat gcctttcgaa ttgaattttt tggtgatgaa
    11521 attgatcgta taagagaaat agaatcgtta acagggcgtg tgctaggaga agtggaacat
    11581 ttagctattt tcccagcaac acactttatg acaaatgatg aacatatgga agaagctatt
    11641 tcaaaaatac aagctgaaat ggaaaatcaa gtagaacttt ttgaaaaaga agggaaattg
    11701 attgaagcgc aacgtattcg acaaaggaca gaatatgata ttgaaatgct tcgtgaaatg
    11761 ggatatacaa atggtgttga gaactactca cgtcacatgg atggtagaag tgaaggtgag
    11821 ccacctttta ctttgttaga tttcttccca gaagattttt tgattatgat tgatgagagt
    11881 cacatgacaa tggggcaaat caaagggatg tataatggag accgttcgcg taaagaaatg
    11941 ttggttaatt acggttttcg tttaccatct gccttagata accgtccact tcgtcgtgaa
    12001 gaattcgaaa gccatgtcca tcaaattgtc tatgtatctg caacgccagg agactatgaa
    12061 atggagcaaa cagatacagt tgttgagcaa attatccggc caacaggact tcttgatcca
    12121 gaagttgaag ttagaccaag tatggggcag atggatgact tgcttggtga aatcaatttg
    12181 aggactgaaa aaggtgagcg tacctttatt acaacattga caaagcgtat ggcagaagac
    12241 ttaacagatt atctcaaaga aatgggagtt aaagttaagt atatgcacag cgatattaaa
    12301 accttagaac gtacagagat tattcgtgat ctacgacttg gtgtgtttga tgttttaatt
    12361 ggtattaatc tcttacgtga aggaatagat gttcctgaag tttcccttgt tgcaatctta
    12421 gatgctgata aggaaggttt ccttcgtaat gagcgtggtc ttatccaaac aatcggacgt
    12481 gcagcacgta acagtaatgg tcatgttatt atgtatgctg ataaaataac agattctatg
    12541 caaagagcta tggatgagac cgctcgccgt cgtcgtttac aaatggatta taatgaaaag
    12601 catggaattg tgccacaaac aataaaaaaa gaaatccgtg atttaattgc tattactaaa
    12661 agtaatgata gcgataagcc tgaaaaagtt gtcgattaca gtagtttatc taaaaaagaa
    12721 aggcaagcta aaattaaagc tcttcaaaag caaatgcaag atgcagctga attattggac
    12781 tttgagctag ctgcacaaat ccgagatgtt atcttagaac taaaagcaat cgattaaact
    12841 tatctctcat ctggttctct ctttaggaga tagcagaagg ttatagatta ttaaataagc
    12901 tgactctagc aggtagagtt agctttttag tttggttaaa aagaactaat ctaaataaga
    12961 taaaaattct taaaaaatac tcattaatta gcaaatagat attgacaact ctatacaaaa
    13021 gtgcttaaat taagcttgct taacaaataa aataatctaa cttaaaaatg agtggaatta
    13081 tgaaaagaat ttctttaaaa acaaccggtg tactgattgc gtttttagct gtaaatgcgg
    13141 tagcgacctc tgtagtggta gatgcagaca cagtagtccc tcatactagt gcgaatacta
    13201 gtcagctttt agaaaaaaac acaactatca agccttccga agttaaacag tcacgtactg
    13261 aaagtaatgc agtaacccct ccaacaatag cctcgagtaa ctcacaaaag cttgagaata
    13321 aatcatctgt agattcaaat atactaaaga gtgaacaacc tgcacataaa gttgctggtg
    13381 ttaaacaagt cgttcaagaa caaacgaaaa atcaaaaaga tgaaacctct ttacaggcta
    13441 aaaagcaagt acaatcaagt gaaaagttat taaatacttc atcacaacct tcaagtgagg
    13501 aaaacaaaaa cactaataag cctaataagg ctaatgaagt ggatatgaaa aatgtcgtga
    13561 ataatgcttc gacgaagcca actcccccat ctgattctaa agtgtataag cgcgttattg
    13621 taacatttga tcaagtaaat aaggaaaaag tagatgaagc ccttaaagca attccgggca
    13681 ctcgtattaa gttaacttat agtagccttt ttaatggcta ttcattagca gtagatgaaa
    13741 aagaaattga aaaaattggt aagattaaag agataaaaca tattgaagca gttcaagtat
    13801 taaaacctca aatgtttact gctaaccaat ggacacatgt cttagctgca cgtttaaaag
    13861 ctaaacaaaa tagtaaagtt gacggtagag ggctacttat tgcgactatt gatagtggtg
    13921 tagatatcca tcatcctgcc atgcgattgg atgagaatga tccagatgtg atggcaaagc
    13981 gtcgtattaa agatattaag gctccttata caaataaagt tccttttgca tacaattatc
    14041 aacgaggcga taatttagaa ttgaaagacg acgttcagcg ccctcatggt atgcatttag
    14101 caggtatctt agcgggaaat gcaaaggatg gttttaaagg agttgcccct aatgcacagt
    14161 tattagtgta caaaatttca acaaatagag aagatccatc tgcagagtgg accggctctg
    14221 atgcagtctt ccatgcaatg gaagatgccg ctaaaagagg ggcagacgtt attagtttaa
    14281 gctttggata tttgggaaca ggacgtccag gtgaactttg gtatgagaca attaagaagg
    14341 tgactgacaa gggtgttatc gtagtagcag caatgggaaa ctatggtaat tcggcttcat
    14401 ctactaccta cgataatgtc gttgacaact ctttgggaac aaaagatact gctgctatgg
    14461 tgtctgttgc agctgttgat aaggttattg gagttggttc agttcaaaat agtgctcttc
    14521 ttttagatgt tttgaaagtt ggaaatctta aattaggata tgcagaactg ggacataaaa
    14581 gtcaaacaat attaaaaatg ctcaaaaata aaacatttga tctagtctat gttggtaaag
    14641 gccagttgaa aaaagatatc tcagaagagg accttaaaag ttatactgga aaagttatcg
    14701 tcgttgagcg tggaggagag ggcgttaaag ataagctaaa acgttttatc ggaattgcta
    14761 aaggggttat tatgataaat gaacctgctt attatagttc tgggaatttt gatactcacc
    14821 ccatgtttga ttatcactat gatataccag aaggtaaatg ggctattagt ctatcaaaaa
    14881 aagatggtga tcagttgatt gactacatca agaaaaacaa aactgctaaa ttagaatttt
    14941 tacctaaaga aacctttgtt cgccgtccaa atcctacaat ttcaggcttt tctagttggg
    15001 gagtgacacc agatttgcat ttgaagccag atgtagttgc tccaggagaa gatatttatt
    15061 ctacttttaa cgacggtacc tatgggtaca tgtctggaac ttcaatggct acccctcaag
    15121 tagctgccat tagtgcatta gtaaaaagta aagtagctga aataatggca ttaaaacttc
    15181 caatgttagc tggtttgaca aacgttgatt tgacaaagat tgtcttgatg aatacaagtc
    15241 aacctttgaa agatactcta aataaagctt tagaaatttc accacgacgt caaggtgcag
    15301 gtatggttaa cttagaaaaa gctttagcta acgatgttct tgtttttggt gataaaacag
    15361 gatcattctc tataaaagag attaagaacc ataaaagatt taccttgaaa ttggtcaata
    15421 caggacaaaa acgtcgtaac tttagtattg aaccaggagc gattttaaca actaaaattg
    15481 ttgataaagt gactaatcag gggtatgaaa atgtcgtttt aaaggcagtt cacgctaaag
    15541 taatagaggg agcaacttta caaatgggta aagttatctc actagcaccg caatcagcca
    15601 tagagcttga tatagatctt gagataaaaa aacctataca agattttgct gaagggtatt
    15661 tgtatttcaa aactttagat aaagatggac aagatatctc agctccttac atgggcttct
    15721 caggaacatg ggatttagaa ccaatttttg acgcacctat gtgggatagt cgttctaaaa
    15781 ataaattaac gaccttgatg caagcaactt tggttggaca cggaactaac aaattttatg
    15841 aaatcggaca agaaataggt agtaaaaagg tcaatccatc cctatttact atgtctaatg
    15901 tgcgtgtagc tcccgataaa gttgtgcgtg tagcacctcg attttcttta ttacgctcag
    15961 catcagattt tgacctctct attgtatcag aagcaaaaga cgattcacca atacttcgtc
    16021 gtataacagt gaaacatttc gtggagaaat attacaaatc aagaaatgag gaagaagact
    16081 catattatga tgctttgaga acgacagaat catcgctagt ttgggatgga aagttttatg
    16141 atcctaagac tggtgatttt aaagctgcaa agccaggctt atattacttt aagttacgtg
    16201 cacgttcaaa tcctacagat ccttatcaag tccaatacct tccaatttta atagataatg
    16261 aaagaccaac tttttctctt aaaaaagtta ataacggctt tgatatttta acccatgata
    16321 atcatcgtgt taaagaagta attgttcgta ataaagagac acaaaagcct atcacagtta
    16381 aaaaaattgc cgaaaatcac tatcatattg atgagttaac gttaagagaa tctgatctgg
    16441 atataaaagt tgcagactat gctgggaatt caaacaagga aattgcttta tcagaatcag
    16501 aagaagtatc agagaaatct tactctagtg aggaagataa tacattacaa ccacagtcac
    16561 tgtctcatcg caatatgaaa gctcaagcaa ccagttcaga tgatgacgat atctgggatg
    16621 ataacgatga tgaccttaac gaagaaaaag attttgattt tgaagaaaat atttctgata
    16681 tcgaggatgg tttattatta actcgagaaa cggtatattc aattcgaact gtaaataaaa
    16741 ttcgaaatgt tgaaaatgaa agcttggagc atagtgtttc tctcttatta aaagatggct
    16801 atcgtgcaga aataagagtt gttaaccttc atgagaacgc taaagatcca aaacattccg
    16861 acccatatcg tacagtatcc aaccctattt atatgtatgg tgatgactct gacacattta
    16921 aaaccacatg ggtcaaattt atgtctggga ataacatcat gaatttgaag gtttataata
    16981 agaatgataa attggtattt agtcgtggct atcatattac cgttgatgta caacctccaa
    17041 aattagaact ggacaagact aacatagaag aagctgctga caaagataac tttatcactg
    17101 gtcaaatcta tgctcaaaat ggagaaattg aaatcaaagg gcacgtttct gataatgtaa
    17161 aaggttgggg tctttctatt aatggtgata gcgtcgatgt taaatattca agtggtgaat
    17221 ttatcagtaa tagatgagca tttactcata agataaaagc agcagcagga gatattgtta
    17281 cgatttcagc ggtagataca tcaggtaata ccgtttctga acattataag gtagttaatg
    17341 atgtggatac accgaagtta actattaatg aagcagattt attatccgga aaagtcaaag
    17401 tggttatagc taaagcagaa gataatcacc ttaaaaattt aatgataact gttaatggca
    17461 agccctacac agaaggacaa agtattgact cttaccaatt aaaaggtttc gaagggcgtt
    17521 acgtcatcaa agtatcagca gttgataaat caggtaaagt cactgaaaaa attataacaa
    17581 tcggcaactt ccctgaatta tcagcaagtt tacgcaaaac ctctttccaa atgcaagatc
    17641 ttacctctat cgataattta ttatccctag cccacaatca gactgcggaa ttgattaagc
    17701 cagtccaact catggacaaa aaagtgagct ttacggcaaa aatatctgac ccccttggtc
    17761 gttctgtggt tagagagttt gaagcattat tatcaacttc aaaccttaaa gtagattcaa
    17821 aactaaaacc acatttgaaa aaacaaactc tccttttaac agatccaaaa ggaattacgg
    17881 accttttcga cttggataag gatagctcta ttttattgac agaaatagtc gataataaag
    17941 tgggaaatca tactataaag ggtttgatat ttaaaaatgg tgcatataac catttcgaaa
    18001 caagtataca aattgtaaaa acaatggaag aaatcaactc tgacagacgt ttgaaaaatg
    18061 atcataaaag ctctgacgac acaaaactaa aaaaagaaac agagtcgtta aaagggcata
    18121 aggttgatcc agcagttcat ggtgaattta aagaaatatc taaacatcaa atggataaac
    18181 cacaagtgat agctgtaggc atgaaaggga gtacaaagac acccccattt gttcatagtc
    18241 aaatggaagc gaagaaaaca actaagattc caatgtctag agcggcaaaa catgaattgc
    18301 caaggacaag tgatgacaat aataatttac tggctaaagg aattgcactc atagtaattg
    18361 tacttggttt actaggcctt aaaaaagctt caaagaaata ttgattttga ttaaattagt
    18421 tttctaattt attaagctaa aattcctttg gataaattat taagggagtt ttaacttaat
    18481 ctattatttt gtagaaaaat tgataaataa atggtattgt aactctatca ctaaatttat
    18541 atactagagg aaataatgtc acgcagtcaa aaagttattt taactaatat gtgtcttatt
    18601 gaagataata aaggacgtat tgtgatgcaa atccgtgatc ctaaacgtta ttcttggtca
    18661 ggagctgctc taccaggcgg ccatattgaa gaaggagaaa gcttgtatga tgctctcgtt
    18721 cgagaagttc gcgaggagac aggtttaaca ataagtaatc ccaaattagt tggtatgaag
    18781 cattggtaca cagatgaagg catccgttat ttggtattcc tttataaaac atatgatttt
    18841 tcaggtgaat tacaatcgtc agatgaaggt gatgttatat ggattgaacg ttcacaactt
    18901 gataagattg aactagcata tgatatgctt aaccttttga gagtatttga cgaagatgac
    18961 atctcagaga tgttttatag taagcgactt gaagatggtt ttataagaaa aattttatta
    19021 aacaaaaaag aactagga
//
LOCUS       DATRUK010000038        17781 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.038, whole genome shotgun sequence.
ACCESSION   DATRUK010000038 DATRUK010000000
VERSION     DATRUK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17781
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.038"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..388
                     /locus_tag="VBE34_001274"
     CDS             <1..388
                     /locus_tag="VBE34_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEO7263993.1"
                     /translation="HQLDVKTTRNQWLGTDQHESDFYAFEDINIIRISGTDTIDLTNV
                     IVSGQDNVIIIQKVFGDTKVLVPLDVAVKADISSVYGSVQYFDFEEYDLRNESIKLSQ
                     EEEYYLLKRVKLVVNTIAGKVEVSRK"
     gene            385..1404
                     /locus_tag="VBE34_001275"
     CDS             385..1404
                     /locus_tag="VBE34_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7263994.1"
                     /translation="MKKHHYFLAFFYGSVIIFAICFVIIDSLGVNLVHLYQTSRLWLI
                     EQLIFSIFFLSLAVTILLLLTWFLLDDNSKRQINHNLRRILNNQSINVTDDGTEISTN
                     IQRLSKKMNLMTASLQSKENSRILKSQEIVKQERKRIARDLHDTVSQDLFAASMVLSG
                     IAQNVSQLDVDQVGSQLLAVEEMLQHAQNDLRILLLHLRPVELENKTLSEGFKMILKE
                     LTDKSDIEVVYHESILTLPKKIEDNIFRIGQEFISNTLKHSQASRLEVYLNQTENELQ
                     LKMIDNGIGFDMDSVYDLSYGLKNIEDRVEDLAGNLQLLSQPGKGVAMDIRLPLVNQS
                     EDKNG"
     gene            1397..2038
                     /locus_tag="VBE34_001276"
     CDS             1397..2038
                     /locus_tag="VBE34_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7263995.1"
                     /translation="MDKIKIVLVDDHEMVRLGLKSFLNLQADVEVIGEASNGLEGIKK
                     ALELRPDVVVMDLVMPEMDGVEATLALLKDWPEAAILVLTSYLDNEKIYPVIEAGAKG
                     YMLKTSSAAEILNAIRKVARGEQAIENEVDKKIKAHDKCPALHEGLTARERDILNLLA
                     KGYDNQRIADELFISLKTVKTHVSNILGKLNVADRTQAVVYAFQHHLVPQDDE"
     gene            2080..3480
                     /locus_tag="VBE34_001277"
     CDS             2080..3480
                     /locus_tag="VBE34_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEO7263996.1"
                     /translation="MDAKTKYKAKKIKAVFFDIDDTLRVKDTGYMPPSILKVFKALKD
                     KGIVVGIASGRARYGVPKEVQDLNADYCVKLNGAYVKDKDKNIIFHRPIPAEYVEQYK
                     KWADTVGIKYGLAGRHEAVLSDRDDLVNDAIDIVYSDLEVNPDFNKEHDIYQMWTFED
                     KGDSLHLPEPLDEHLRLIRWHDHSSDVVLKGTSKALGVSKVVEHLGLKPENILVFGDE
                     LNDLELFDYAGLAVAMGVSHPEAQKKADFITKKVEEDGILYALEELGLIEKELTFPQV
                     DIENTEGPVAVIKTNHGDMTVKLFPDHAPKTVANFIGLAKQGYYDGIIFHRIIPDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNVRGALSMANAGPNTNGSQFFIVQNTKIP
                     YAKKELERGGWPTPIAELYAGQGGTPHLDRRHSVFGQLVNQSSFEVLDEIAAVETGSQ
                     DKPLEDVVILTIEVKE"
     gene            3482..3838
                     /locus_tag="VBE34_001278"
     CDS             3482..3838
                     /locus_tag="VBE34_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEO7263997.1"
                     /translation="MKIGDKLKGTVTGIRPYGAFVSLEDGRTGLIHISEIKTGYIDNI
                     YDVLSVGDEVYVQVIDVDEFTQKASLSLRTLEEERHHIQHRHRFSNNRLKIGFKPLEE
                     NLPSWVEEGLAYLNNK"
     gene            complement(3884..4660)
                     /locus_tag="VBE34_001279"
     CDS             complement(3884..4660)
                     /locus_tag="VBE34_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEO7263998.1"
                     /translation="MTNEKGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQ
                     KMVPETMRDAITNESVIVGEEKSVDDIIEEVLKDIDFYEESGGGITLSGGEIFAQFEF
                     AKAILKRAKSLGIHTAIETTAYTRHEQFIDLIQYVDFIYTDLKHYNSLKHQEKTMVKN
                     ASIIKNIHYAFANGKTIVLRIPVIPNFNDSLEDAEEFACLFDRLDIRQVQLLPFHQFG
                     QNKYRLLNRQYEMEEIAALHPEDLLDYQAIFSKYNIHCYF"
     gene            4781..5536
                     /locus_tag="VBE34_001280"
     CDS             4781..5536
                     /locus_tag="VBE34_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO7263999.1"
                     /translation="MNRLENIISLVSQYQKIDVNTLSELLQVSKVTIRKDLDKLEGKG
                     LLHREHGYAVLNSGDDLNVRLSFNHKTKKEIAALAANMVSDNDTILIESGSTCALLAE
                     NICQTKRNVTILTNSCFIANYLREYDSCQIVLLGGEYQSSSQVTVGPLLKKMISLFHV
                     SLAFVGTDGFDPKLGFMGRDLMRSEVAQEMANAADEVIILTDSSKFNQTALVEQLPLS
                     TVSQVITDKHPNSDIANLFQEAEITIQSLLQVP"
     gene            5655..6638
                     /locus_tag="VBE34_001281"
     CDS             5655..6638
                     /locus_tag="VBE34_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEO7264000.1"
                     /translation="MKLERQKLLAKVAYLYYMEGKSQSEIANELGIYRTTISRMLAKA
                     REEGLVRIEISDFNPEIFQLESYFKSKYHLKDIEIVSSRKDSDTSEIEKDLAHVAAAM
                     IRKKIKENDKVGIAWGRTLSKVVEAMRPHPVSQVSFVPLAGGPSHINARYHVNTLVYE
                     MSRRFQGSCTFINATLVQENANLAKGILTSKYFEGLMDNWEKLDVAIVGVGGKPKSNE
                     QQWLDLLNQEDFQCLDEEAAVGEITCRFFNHSGDSVNQHLAKRTIGITLEQLQKVPNR
                     IAVAHGNYKAAALLAVLKKGYINHLVTDFSTALNILRLDKDTFVDTMYQKS"
     gene            6842..7165
                     /locus_tag="VBE34_001282"
     CDS             6842..7165
                     /locus_tag="VBE34_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEO7264001.1"
                     /translation="MEIIVADQIIMGLILNAGDAKQHIYQALKLAKEGNFAESKIEIE
                     LADSALLEAHNLQTQFLAQEAGGTRTDISALFIHSQDHLMTSITEINLIKEIIDLRQE
                     LHIQK"
     gene            7182..7502
                     /locus_tag="VBE34_001283"
     CDS             7182..7502
                     /locus_tag="VBE34_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7264002.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKIAADKEGIEAHIEAYSQGKIAD
                     YAKDLDVALLGPQVSYTLDKSKSICDEYGVPIAVIPMADYGMLDGVKVLKLALSLLET
                     RKEA"
     gene            7504..8805
                     /locus_tag="VBE34_001284"
     CDS             7504..8805
                     /locus_tag="VBE34_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7264003.1"
                     /translation="MSKFDSQKIITPIMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     LGQLPFKGLNQAIANVFGPEWTEPFMQVYSGTFAIMGLISCFAIAYAYAKNSGVEPLP
                     AGVLSLSSFFILMKSSYIPVKGEAIADAISKVWFGGQGIIGAIIIGLVVGAIYTWFIQ
                     HHIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYLIAKVTTGGTFIEMIYDIIQVP
                     LQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKSLLAANRLTL
                     DNGAHIVTQQFLDSFLILSGSGITFGLVIAMLFAAKSKQYKALGKVAAFPAIFNVNEP
                     IVFGFPIVMNPVMFLPFILVPVLAALIVYGAIAVGFMQPFSGVTLPWSTPAIISGFMV
                     GGWQGALVQIVILAISTAVYFPFFKIQDNITYKNECEMERG"
     gene            8986..11442
                     /locus_tag="VBE34_001285"
     CDS             8986..11442
                     /locus_tag="VBE34_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEO7264004.1"
                     /translation="MTKQLLTQKYTNDVQQNSQKHFGYLTERMYSYRDKVLDKKPFID
                     AERAILVTEAYQKHQEKPNVLKRAYMLQNILEKMTIYIDDETMIVGNQASSDKDAPIF
                     PEYTLEFVVNELDLFEKRDGDVFYITEETKEQIRNIAPFWENNNLRARAGVMLPEEVQ
                     VYMETGFFGMEGKMNSGDAHLAVNYQKLLEEGLIGFEKKARKAKADLDLTKPESIDKY
                     HFYDSILITIEAVKTYAERFAILAKKQAKTANTKRRQELLDIASICERVPYYPAETFA
                     EAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDSIVERLTNLWI
                     KTITINKVRSQAHTFSSAGSPLYQNVTIGGQTRHKEDAVNPLSFLVLKSVAQTHLPQP
                     NLTVRYHANLDKSFMNEAIEVMKLGFGMPAFNNDEIIIPSFIKKGVSEEDAYDYSAIG
                     CVETAVPGKWGYRCTGMSYINFPKVLLITMNNGIDPASGKRFAPSYGHFTQMTSYKEL
                     KEAWDKTLRYLTRMSVIVENAIDISLEREVPDILCSALTDDCIGRGKHLKEGGAVYDY
                     ISGLQVGIANLSDSLAALKKLVFEEKRLTTLEVWQALQSDYAGPRGEEIRQMLINEAP
                     KYGNDDDYADSLVRECYDVYVEEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGT
                     LATPDGRHAGTPLAEGCSPSHNMDKKGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTL
                     AKEEDKQKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAF
                     FNVLSKATQDDIIARTEHAL"
     gene            11452..12120
                     /locus_tag="VBE34_001286"
     CDS             11452..12120
                     /locus_tag="VBE34_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO7264005.1"
                     /translation="MEFLLDTLNLEAIKKWHHILPLAGVTSNPTIAKKEGDIHFFQRI
                     RDVREIIGCEASLHVQVVAKDYQGILDDAAKIRQETDDDIYIKVPVTPDGLAAIKTLK
                     AEGYNITATAIYTSMQGLLAISAGADYLAPYFNRMENLDIDATQVIKELAQAIERTGS
                     SSKILAASFKNASQVTKALSQGAQSITAGPDIFESVFAMPSIAKAVNDFADDWKASQH
                     SEHI"
     gene            12188..13276
                     /locus_tag="VBE34_001287"
     CDS             12188..13276
                     /locus_tag="VBE34_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEO7264006.1"
                     /translation="MKVFASPSRYIQGKDALFQSIEHIKSLGQTPLILCDDVVYNIVG
                     ERFLSYLQDDLLPHRVSFNGEASDNEINRVVAVAKEKNSDLIIGLGGGKTIDSAKAIA
                     DKVNLPVIIAPTVASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVIAQAPK
                     RLLASGIADGLATWVEARAVLQKNGIAMAGGRQTLAGVAIAQACERTLFNDSLQALAA
                     CDVKVVTKALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALSGDIHHLTHGEKVAY
                     GTLTQLFLENRPKEEIDRYINLYQAIGMPTTLAELHLGDATYEELLKVGQQATIEGET
                     IHEMPFKISAEDVAAALLAVDRYVSNHQ"
     gene            complement(13428..14354)
                     /gene="cysK"
                     /locus_tag="VBE34_001288"
     CDS             complement(13428..14354)
                     /gene="cysK"
                     /locus_tag="VBE34_001288"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEO7264007.1"
                     /translation="MSKIYNSITDLIGNTPIIQLHHIVPEGAAEVYVKLESFNPGSSV
                     KDRIALAMIEDAEQKGILKAGDTIVEPTSGNTGIGLAWVGKAKGYNVIIVMPETMSIE
                     RRKIIQAYGAQLVLTPGSEGMKGAIAKAKEISAEQNAWLPLQFNNQANPEIHEKTTGR
                     EIIETFGEKGLDAFIAGVGTGGTITGVSRALKKVNPDVAIYAVEADESAILSGEQPGP
                     HKIQGISAGFIPETLATDSYDHIIRVTSNDAIETGRIIGGLEGFLAGISASAAIYAAI
                     EVAKQLGKGKKVLALLPDNGERYLSTSLYDFE"
     gene            complement(14445..15089)
                     /locus_tag="VBE34_001289"
     CDS             complement(14445..15089)
                     /locus_tag="VBE34_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEO7264008.1"
                     /translation="MNYKTIKSDGIVEEEIKKSRFICHLKRVESEEEGRNYITQIKKA
                     HYKANHSCSAMVIGEKGDIKRSSDDGEPSGTAGIPMLTVLEKQGLTNVVAVVTRYFGG
                     IKLGTGGLIRAYSGSVANAIKEIGVVEVKEQIGIRIQLTYPQYQTFDNFLKEHHLQEF
                     ETEFLEAVTCKIYVDPKEFEHTITNLTEFYQGKALLTEEGSQIVEIPFKAETDK"
     gene            15145..16434
                     /locus_tag="VBE34_001290"
     CDS             15145..16434
                     /locus_tag="VBE34_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7264009.1"
                     /translation="MENYLGRLWTKAQLSEQLRKIAINLPSFIKKGSDYICTRCSSSV
                     AKNCQLPTGNYYCRECIVFGRVTSNENLYYFPQKTFSKTNSLKWKGGLTPYQNEVSEE
                     LLKGISSKENLLVHAVTGAGKTEMIYHSIAKVIDTGGSVCIASPRIDVCLELHKRLSN
                     DFRCAITLMHGESPSYQRSPLTIATTHQLLKFYHAFDLLIVDEVDAFPYVDNPILYHG
                     VKQALKKNGTSIFLTATSTTELERKVARKELKKLHLARRFHANPLVIPEMVWVSGIQK
                     SLQTQKLPPKLYQLINKQRQTRYPLLLFFPHIAEGQVFTEMLRQAFPMEKIGFVSSKS
                     TSRLKLVQDFRDNKLSILVSTTILERGVTFPSVDVFVIQANHHLFTKSSLVQISGRVG
                     RALERPEGLLYFLHDGKSKSMHQAIKEIKNMNHIGGF"
     gene            16434..17100
                     /locus_tag="VBE34_001291"
                     /pseudo
     CDS             16434..17100
                     /locus_tag="VBE34_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
     gene            17177..17731
                     /gene="raiA"
                     /locus_tag="VBE34_001292"
     CDS             17177..17731
                     /gene="raiA"
                     /locus_tag="VBE34_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEO7264010.1"
                     /translation="MIKYSIRGENIEVTEAIREYVETKLSKVEKYFNEEQELDTRVNL
                     KVYREKTAKVEVTILIDSITLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKYRE
                     KIPASQVFTTEFEAEPDEEAVSQRIVRTKNVNLKPMDVEEALLQMELLGHDFFIYTDA
                     EDNTTNVLYKREDGELGLIEAKGN"
BASE COUNT         5868 a         2860 c         3489 g         5564 t
ORIGIN      
        1 ccatcaatta gatgttaaga caacacgaaa ccaatggtta ggtactgatc agcacgaaag
       61 tgacttttat gcttttgaag atattaatat tataagaatc tctgggactg atacaattga
      121 tttaacaaat gttattgtat ctgggcagga taatgttatt ataattcaaa aagtttttgg
      181 tgataccaaa gtattggttc ctttagacgt tgccgtaaag gctgatatta gttctgttta
      241 cggtagtgta cagtactttg attttgagga gtatgatcta agaaatgaat ctataaaatt
      301 gtcacaagag gaagaatatt accttttgaa acgtgtcaaa ttagttgtaa acactatcgc
      361 tgggaaagtt gaggttagtc gtaaatgaaa aagcaccact actttttagc tttcttctat
      421 ggaagtgtta ttatatttgc catttgtttt gtcattattg atagtttggg tgttaatttg
      481 gtgcacctct atcaaacgtc tcgtttgtgg ctgattgaac aactgatttt ctctattttc
      541 tttctatctt tagcggtaac tatcctttta ttgctaactt ggtttttatt agacgataat
      601 agtaaacgtc aaattaacca taatttacgt cgcattttaa ataaccaatc aataaatgtt
      661 accgatgatg gtactgaaat atcgaccaat attcaacggc tctctaaaaa aatgaattta
      721 atgacagcaa gtctacaatc taaggaaaat agtcgtattt taaaaagcca agaaattgtg
      781 aaacaagaac gtaagcgtat tgcacgtgat ttacacgaca cagttagtca ggatttgttc
      841 gcagcttcca tggttttgtc agggattgca cagaatgttt ctcagctaga cgttgatcag
      901 gttggctcgc aattattagc agtagaggaa atgcttcagc atgcacaaaa tgatttgaga
      961 attctgctac ttcatttgag gccagttgaa ttagaaaata agaccctatc tgaaggattc
     1021 aaaatgatat tgaaagaatt gaccgataag agcgacattg aagttgttta tcacgaatca
     1081 attctaacct tacccaaaaa gattgaagac aatattttta gaattggtca agaatttatc
     1141 agtaatactt taaagcattc acaagccagt cgtctcgaag tgtacctcaa tcaaacagag
     1201 aatgaattgc agttaaaaat gattgataat ggtattggat ttgatatgga ttctgtctat
     1261 gatttaagtt atggattaaa aaatattgaa gatcgagtag aagatttagc aggaaattta
     1321 cagcttctca gtcagcctgg taaaggagtt gctatggata taagattacc tttagtaaat
     1381 cagagtgagg ataaaaatgg ataaaataaa aattgtactt gttgatgacc atgagatggt
     1441 tcgtctcggt ttaaaaagtt ttttaaactt acaagctgat gttgaagtaa taggtgaagc
     1501 ctcaaatggt ttagaaggca taaaaaaagc actggagtta cgtccagacg ttgttgttat
     1561 ggatttagta atgcctgaaa tggacggtgt agaagcaacc ttagcattgt tgaaggattg
     1621 gccagaagct gctattttag tcttaacatc ttacttggac aatgaaaaaa tttaccctgt
     1681 tattgaagca ggtgccaaag gctatatgtt aaagacctca agtgcagcag agattcttaa
     1741 tgccattcgt aaagttgcaa ggggagaaca agctattgaa aatgaagtag ataagaaaat
     1801 taaggctcat gataaatgtc cagctttaca tgagggctta acagcaaggg aacgtgatat
     1861 ccttaattta ttagccaaag gttatgacaa tcaaaggatt gctgatgaat tatttatctc
     1921 gttaaagact gttaaaactc atgtttctaa tattttggga aaactaaatg tggcagatcg
     1981 aactcaagcg gttgtctatg ctttccaaca ccatttggta ccacaagatg atgaataagt
     2041 gatataatag agacactatg ttaatgaaag tggttagtca tggacgcaaa aactaaatat
     2101 aaagcaaaaa aaataaaggc ggtgtttttt gatattgatg acacgcttcg tgttaaagat
     2161 acaggttata tgccaccatc gatcttaaaa gtttttaaag ctttgaaaga taaaggaatt
     2221 gttgtaggta ttgcttcagg aagggcacga tatggcgttc caaaagaagt tcaagattta
     2281 aatgccgatt attgtgtaaa attaaatgga gcttatgtta aggacaagga taagaatatc
     2341 atcttccatc gtcctattcc tgctgaatat gtagagcaat ataagaaatg ggcagacact
     2401 gttggtatta agtacggttt agctggtcgt catgaggcag ttttatcaga tcgtgatgat
     2461 ttggtaaatg atgcgattga tattgtgtat tctgatctcg aagttaatcc agatttcaat
     2521 aaagaacatg atatttatca gatgtggacg tttgaagata aaggagatag ccttcatcta
     2581 cctgaaccgt tagatgaaca tcttcgcttg atacgttggc atgatcattc ttcagatgtt
     2641 gtgttaaaag gaacatcgaa ggctttaggt gtttctaaag ttgttgaaca tttgggctta
     2701 aagccagaga atatcttggt tttcggagat gagctcaatg atttagaact ctttgattat
     2761 gctggactag ctgtggcgat gggagtgtca cacccagaag ctcaaaaaaa agcagatttt
     2821 atcacaaaaa aagtagaaga agatggcatt ttgtatgccc tggaggaact aggtttgata
     2881 gaaaaagaat taacatttcc acaagtagat attgaaaata cagaaggtcc tgtggctgtc
     2941 attaaaacaa atcacgggga tatgactgta aaactcttcc cggatcatgc tcctaaaacg
     3001 gtagcaaact ttattggttt agctaaacaa ggttattatg atggtattat cttccatcgt
     3061 attattccag attttatgat tcagggtggt gatccaactg gtacaggtat gggtggtgaa
     3121 tctatctatg gggaaagttt tgaagacgaa ttttccgaag aattatataa tgttcgcgga
     3181 gctctttcaa tggctaatgc tggaccaaat acgaatggca gccaattttt catcgtccaa
     3241 aatacaaaaa ttccttatgc gaaaaaggaa ttagagcgag gtggttggcc aacaccaatt
     3301 gctgagttgt atgctggaca aggtggtaca ccgcatctag atcgtcgcca cagtgtattt
     3361 ggacaattag taaaccaaag ttcttttgag gtacttgatg agattgcggc agtagagact
     3421 ggttctcaag acaaaccatt ggaagatgtt gttattttaa cgattgaggt taaagaataa
     3481 aatgaagatt ggcgataagt taaagggaac agtcactggc attaggcctt atggagcctt
     3541 tgtttctctt gaggatggta ggactggact tatccatatt tctgaaatta aaacaggtta
     3601 cattgataat atttatgatg ttcttagtgt aggtgatgaa gtctatgttc aggttataga
     3661 tgtagatgaa tttacacaaa aagcaagttt atcgcttcgg acccttgaag aagaaagaca
     3721 tcatattcag catcgtcatc gattttctaa caatcgtctt aagataggtt tcaaaccact
     3781 tgaggaaaat ttacctagtt gggttgagga aggtctagcc tacttgaata ataaataaac
     3841 aaaaacctag atttgattaa tgacaagtct aggttttaaa tatttaaaag tagcagtgaa
     3901 tattatactt gctaaaaata gcttgataat cgagtagatc ctctgggtgt agtgcagcga
     3961 tctcttccat ttcatattga cgattaagta atcgatattt gttttgtcca aattggtgga
     4021 aaggtaagag ttgcacttga cggatatcta ggcgatcaaa taaacaagca aattcttcag
     4081 catcttctaa tgaatcgtta aaattaggta tgacaggaat acgtagcaca atcgttttac
     4141 cgttggcaaa agcataatga atgtttttga tgattgaagc attcttaacc atcgtctttt
     4201 cttggtgttt gaggctattg taatgtttta aatcggtgta aataaaatca acgtattgga
     4261 taaggtctat gaattgttca tgtctagtat aagcagttgt ttcaatagcc gtgtggattc
     4321 ccagtgattt agcacgtttt aaaattgcct tggcaaattc aaattgtgca aatatttcac
     4381 cgccagatag cgttatgccc ccaccagatt cttcgtaaaa atcaatatct tttaaaacct
     4441 cttcaataat atcatcaacc gatttttctt cgcccacgat gacgctttca ttagtaatgg
     4501 catctctcat tgtttctgga accatttttt gcgactcggg attagcacac caaggacatc
     4561 taagaggaca accctttaag aaaacggttg ttcgaatacc tggaccatcg tgaatactaa
     4621 aatgttgaat gttgaacaca ataccttttt catttgtcat attattttcc tcaatttcat
     4681 tttaagtata ccttttatac gtaacaaagt caattacgaa tgaaagttta ttgaacttgt
     4741 ttaataaata aaaaatgata aaatagaaag gaggtgtttc atgaatagat tagaaaatat
     4801 tattagttta gtatcccagt accaaaaaat agatgttaat acactctcgg agttgttgca
     4861 agtttctaaa gtaaccatac gtaaagattt agataaacta gaaggcaaag ggcttttgca
     4921 tagagagcac ggttatgcag ttttaaacag tggggatgat cttaatgtta ggttatcttt
     4981 taaccataaa actaaaaaag aaattgctgc cttagctgca aatatggtgt ctgacaatga
     5041 cactatctta atagaatcag gctctacttg tgctctttta gcggaaaata tctgtcagac
     5101 aaagcgaaat gtcactattt tgaccaactc ttgctttatt gctaactatt tacgagagta
     5161 tgattcatgc caaattgtct tacttggtgg tgagtatcaa tcaagttcac aggttacggt
     5221 aggtcctctc ttgaaaaaga tgatttcact ttttcatgtc tcgttagctt ttgttgggac
     5281 tgatggtttt gatccaaaac taggatttat gggtagagat ttaatgcgtt ctgaagttgc
     5341 acaagagatg gcgaatgctg ccgatgaagt cattatttta acagattcta gtaagtttaa
     5401 tcagactgca ttggtggaac agcttccgtt gtcaactgta tcacaagtta ttacagataa
     5461 acatccaaat tcggatattg ctaacttgtt ccaagaagca gagattacca ttcaatcttt
     5521 attacaagta ccataaaaga ggctattttt cttacatttt gcacatttgt ggcgataata
     5581 gataaagtgt catgtcaaat tgcacagatg taaccttttt gttaaactga agcaaatgct
     5641 gaaaggttat gagtatgaaa ttagagcgtc aaaaattgtt agctaaagta gcttaccttt
     5701 actatatgga agggaaaagc caatctgaga ttgccaatga attaggaatt tatcgtacaa
     5761 caataagtcg tatgttagcc aaagcgagag aagaaggtct tgttagaatc gaaatttcag
     5821 attttaatcc agaaattttt cagttagaat cctattttaa aagtaagtat caccttaaag
     5881 atattgagat tgtttcttcg cgaaaagata gcgatacttc agagatagag aaggatttag
     5941 cacatgtagc tgcagctatg attcgaaaaa aaattaaaga gaatgacaag gtggggattg
     6001 cttgggggag gacacttagt aaagtagttg aagcaatgcg tcctcatccg gtttctcaag
     6061 tttcttttgt tcctttggcc ggaggtccaa gtcatattaa tgcacgctat cacgttaata
     6121 ctctggttta cgaaatgtca aggcgtttcc aaggaagttg tactttcatt aatgcaactc
     6181 tagttcaaga aaacgctaat ttagcgaaag gaatattaac ttcaaaatat tttgaggggc
     6241 taatggataa ttgggaaaaa cttgatgtgg caattgtggg cgttggtggt aaacctaaaa
     6301 gcaatgaaca acagtggctt gatcttttaa atcaagaaga ttttcaatgc ttggatgaag
     6361 aagcagctgt aggtgaaatt acttgtcgtt tttttaatca ttcaggtgat tctgtgaatc
     6421 aacatttagc taaaaggaca ataggaatta ccttagaaca actacagaaa gttcctaata
     6481 gaatagcggt tgctcatggc aattataagg ctgcggcact cttagccgtt ttaaaaaagg
     6541 ggtatattaa tcatttagtt actgattttt ccacagcctt aaatatttta agattagata
     6601 aagacacctt tgtggatact atgtatcaaa agagctagga tttattccta gctcttttaa
     6661 taatgtttct attaccaagt gaattgttat agaataagca caaaagtgcg ctattatcat
     6721 aaagggcaca aggtctattg acgacgatta agggacatgt tacatttgtt ttgcgaaaga
     6781 aaattagaac cttttgtaag aaagtttaaa cttataaaaa atcattaaag cggaggtgct
     6841 tatggaaatc atagtcgcag accaaattat tatggggctc attttgaatg ctggggatgc
     6901 taaacaacat atctatcaag ccttaaaact tgcaaaagaa ggaaattttg cagaaagtaa
     6961 aattgaaata gaactagcag atagtgcact cctagaagct cataatcttc aaacacaatt
     7021 ccttgcacaa gaagcaggag ggacaaggac agatatctca gcactcttta ttcattctca
     7081 ggatcatttg atgacaagta ttactgagat taacttgatt aaagaaatta ttgatttacg
     7141 ccaggaatta catattcaaa aataagtaaa ggggatcaaa tatgataaaa attggtttat
     7201 tctgtgcagc aggtttttca acaggtatgt tagtaaacaa tatgaaaatc gcagcggata
     7261 aagaagggat agaggcacat attgaagctt attcacaagg aaaaattgca gattatgcta
     7321 aagatttaga tgttgcatta cttggtccac aagttagcta tactctagat aaatcaaaat
     7381 caatctgtga tgaatacggt gtaccgatag cagttattcc aatggcagat tatggtatgt
     7441 tggatggtgt aaaagttttg aaacttgctt tgagtttatt ggaaacaaga aaagaggctt
     7501 gatatgtcaa agtttgatag tcagaaaata attactccga ttatgaagtt tgtcaatatg
     7561 cgagggatta ttgcactcaa agatggcatg ctagcaattt taccactaac agttgttggg
     7621 agtctctttt taatattagg gcagcttcca tttaaaggtc ttaatcaagc catagctaat
     7681 gtttttggac cagaatggac agaaccattt atgcaagttt attcaggaac ttttgcgatt
     7741 atgggcttga tttcctgttt tgcaattgct tatgcttatg ctaaaaatag tggtgtagaa
     7801 ccgttacctg ctggcgtttt atcactttcg tctttcttta ttttaatgaa atcatcttat
     7861 attcctgtaa aaggagaagc aatagcggac gctatttcta aggtttggtt tggcggtcag
     7921 gggattattg gagcaattat tattggctta gtagttggtg ctatctatac atggtttatc
     7981 caacatcata ttgttataaa aatgccagag caagtaccac aggcaatagc aaaacaattt
     8041 gaagctatga ttccagcttt tgttatcttt cttttatcga tgattgttta tttgattgct
     8101 aaggtaacaa ctggaggtac ctttattgag atgatttatg atatcattca agtaccgttg
     8161 caaggcttaa caggctcact ttatggagca attgggattg ctttctttat ttcattctta
     8221 tggtggtttg gtgtccatgg ccaatctgtg gtgaatggta ttgtgactgc tttgttacta
     8281 tccaatttag atgccaataa gtctttatta gcagcaaatc gcttaacatt agataatggc
     8341 gctcacattg taacacagca gtttttggat agtttcttga ttttatcggg ctcaggaata
     8401 acttttggac tagttattgc gatgcttttt gcagcaaaat caaaacaata caaggcactt
     8461 gggaaagtgg ccgcttttcc cgcaattttc aatgttaacg agccaatcgt ctttggcttt
     8521 ccaatcgtaa tgaatcctgt gatgtttctg ccttttattt tagtaccagt tttggcagct
     8581 ttaattgttt atggagctat tgcggttggt tttatgcaac cattctcagg tgttacttta
     8641 ccatggagta ctccagcaat tatttctgga ttcatggtag gtggttggca aggtgcactc
     8701 gtccaaatag tcattttagc tatctcaact gctgtttact tcccattctt taaaatccaa
     8761 gataatatta cttacaaaaa tgaatgtgaa atggaaaggg gatagctagc agataagagg
     8821 ttgaggtgtg taatgcacct taaccttttt ctaaaaatgc acatttgtgc aactttagat
     8881 gtagcattct attctttatt ttcttcttgt tattttagga tttaggtgct attatattta
     8941 cgaagaaaaa ctttttagtt tcgaaagaaa caagcggagg ctgttatgac caaacaatta
     9001 ctcactcaaa aatatacaaa tgatgttcaa caaaattctc aaaagcattt tggctatttg
     9061 acagagcgca tgtattctta tcgcgataag gttcttgata agaaaccatt catagatgca
     9121 gaacgtgcta tcttggtaac tgaagcctat caaaagcatc aggaaaaacc taatgtttta
     9181 aagagagctt acatgttgca aaatattttg gaaaaaatga caatctatat tgacgatgag
     9241 acaatgattg tcggtaacca agcttcatct gataaagatg ctcctatttt tccagaatac
     9301 actctagaat ttgttgtaaa tgaactagac ctttttgaaa aacgagatgg tgacgtcttt
     9361 tacattacag aagaaaccaa agaacaaatt agaaatattg cacctttctg ggaaaataat
     9421 aatttacggg ctcgtgcggg cgttatgctt ccagaagaag ttcaagttta tatggagaca
     9481 ggcttctttg ggatggaagg taagatgaat tctggggatg ctcacttagc tgttaactat
     9541 cagaagttac tagaagaagg gcttattggc tttgaaaaaa aagcaagaaa agctaaagct
     9601 gacttagatt taacaaagcc tgagagtatt gacaaatatc atttctatga ttctatttta
     9661 attacaatag aagctgttaa aacctatgca gagcgttttg ctatcctagc aaaaaaacaa
     9721 gctaaaacag caaatactaa gagacgtcag gaattgttag atatcgctag tatttgtgaa
     9781 cgagtgccat attacccagc tgaaacgttt gcagaagctg ttcaatcggt ttggtttatt
     9841 cagtgtattc ttcaaataga atcaaacggt cattctctat cttatggacg ctttgatcaa
     9901 tacatgtatc catatgttaa agctgactta gaagctggac gtgagactga ggattcgatc
     9961 gttgagcgcc taactaacct atggattaaa acaataacta ttaataaggt tcgcagccaa
    10021 gctcatacgt tttcgtctgc tggtagtcct ctttaccaaa atgtaacgat tggaggacaa
    10081 acccgacata aggaagatgc tgttaatcct ttatcatttc tagtactaaa atcagtcgct
    10141 cagacacact taccacagcc taatctaacg gttcgttatc atgctaacct agacaagtca
    10201 ttcatgaacg aagctattga agttatgaaa ttaggttttg gtatgccagc ttttaataat
    10261 gatgaaatta ttattccatc atttattaaa aaaggagttt ccgaagaaga tgcctatgat
    10321 tattcagcga ttggttgtgt tgaaacagca gttcctggga aatggggtta tcgttgtacc
    10381 ggcatgagct atatcaactt ccctaaagtg ttattaatta caatgaataa tggcattgac
    10441 ccggcttctg ggaagagatt tgcgccttct tatgggcatt tcacccagat gacctcatat
    10501 aaagaactta aagaagcttg ggacaaaaca cttcgttact taactcgaat gagtgttatt
    10561 gttgaaaatg ctattgatat tagtctcgaa agagaagtac ctgatattct ttgttcagct
    10621 ttaactgatg attgtattgg acgaggtaag catttaaaag aaggtggtgc agtatatgac
    10681 tacatatctg gtctacaagt tggaatcgct aatctttcag actcacttgc tgcgcttaaa
    10741 aaattagtat ttgaagaaaa acgcctaacc acattagaag tatggcaagc attgcagagt
    10801 gattacgcag gtccgcgtgg tgaagaaatt cgtcaaatgt tgatcaatga agcaccaaaa
    10861 tatggaaatg atgatgacta cgcggatagt ttagtacgtg agtgttatga tgtttatgtt
    10921 gaagaaattg ctaaatatcc caacacacgc tatggacgtg gaccaatcgg tggtattcgc
    10981 tattcaggaa cgtcctctat ttcagcaaat gtaggtcaag gacgaggtac tttagcaact
    11041 ccagatggta gacatgcagg aactccatta gctgaaggtt gttcaccatc tcataatatg
    11101 gacaaaaagg gacctacctc tgttttgaaa tcagtttcga agttaccaac tgatgagatt
    11161 gtcggcggtg tactccttaa ccaaaaagtc aaccctcaaa ccttggcaaa agaagaagat
    11221 aaacaaaaat taattgcctt acttagaaca ttcttcaatc gccttcatgg ttaccatatc
    11281 cagtataatg ttgtctcacg tgagactttg attgatgctc agaagcaccc tgaaaaacac
    11341 cgcgatttga ttgtgcgtgt agcaggttat tctgcattct ttaatgtcct atctaaggca
    11401 actcaagatg atattattgc acgtacagaa catgctcttt aggagattat tatggaattt
    11461 ttacttgaca ctttaaattt agaagctata aaaaaatggc atcatatact gccactagca
    11521 ggtgtaactt caaatcccac cattgctaag aaagaaggag atatacattt tttccaacgc
    11581 attcgtgatg ttcgagaaat tattggatgt gaagcaagtt tgcatgttca agtagtggcc
    11641 aaagattatc aagggatact ggatgatgct gctaaaatac gccaagaaac ggatgatgac
    11701 atttatatca aggttccagt tacgccagat ggattagcag caattaaaac gttaaaagca
    11761 gaaggttata atattacagc cacagcaata tacaccagta tgcaaggtct tttggctata
    11821 tcagctggag cagactacct agcaccttac tttaaccgaa tggagaacct tgatattgat
    11881 gcgacacaag ttattaagga attagcgcaa gcaatagaga ggacaggttc ttctagcaaa
    11941 attttagcag ctagttttaa aaacgcttca caagtaacga aggccttatc acagggtgct
    12001 caatcaatca ccgcgggacc agatattttt gaatcagtct ttgcgatgcc atctattgca
    12061 aaagctgtta acgactttgc agatgattgg aaagccagtc aacactcaga acatatttaa
    12121 cagagaacgc caacttttga ggctgtatct ttaacataaa attttttaaa gaagaaagag
    12181 gttgagaatg aaagttttcg caagtccatc gcgctatata caaggaaagg atgctttatt
    12241 ccaaagtatt gaacatatca aatctttagg tcagactcca cttatccttt gtgatgatgt
    12301 tgtttataat atcgttggag aaaggttttt atcttatcta caagacgatt tgttaccaca
    12361 tcgtgtctcc tttaacggag aagcatctga taatgaaatc aatcgtgtcg ttgctgttgc
    12421 aaaagaaaag aatagtgacc ttattattgg acttgggggt ggaaaaacta ttgactctgc
    12481 aaaggcaatt gcagataaag ttaatttacc agtgatcatt gcaccaacag tagcttcaac
    12541 agatgcccca acatcagcgt tatcagtaat ctatacagat gaaggagctt ttgaaaaata
    12601 tattttttat tcaaagaatc cagacttagt tttggtcgat actcaagtca ttgctcaagc
    12661 gccaaaacgt ttactagctt ctgggattgc agatggctta gctacttggg ttgaagcgag
    12721 agcagtctta caaaaaaatg ggatagcgat ggctggcggt agacagactt tagcgggcgt
    12781 ggcaattgct caagcatgtg agcgtacttt atttaatgat agtttacaag ctttagcggc
    12841 ttgtgatgtc aaagtggtta caaaagcttt agaaaatgtt attgaagcta ataccttact
    12901 tagtggttta ggttttgaaa gcgcaggatt agctgcagct catgctattc ataatggttt
    12961 tactgccttg agtggagata ttcatcactt aacccatggt gaaaaagttg cctatggaac
    13021 gttgacacaa cttttcctcg aaaatagacc aaaggaagaa attgatcgct acattaatct
    13081 ttaccaagcg attggaatgc caactacact tgcagaactc catttgggtg atgcaacata
    13141 tgaagaactt ttaaaagttg gtcagcaagc aacaattgaa ggtgaaacaa tacatgaaat
    13201 gccattcaaa attagtgcag aggatgtcgc tgcagccctg ttagcagttg accgctatgt
    13261 ttcaaatcat caataaaaac aatagaacca agtgaaattg cttggttcta tttgtcgttt
    13321 taatatttta actaaatagg gcttactagt atttaaagag atataaacta aaaaagatca
    13381 gacaacatat ctaatctttt ttagttatcg tttactgaag attaaagtta ttcaaaatca
    13441 tacaggcttg ttgataagta acgttcgcca ttatcaggga gaagagctaa aacctttttc
    13501 ccttttccta gttgtttggc cacttcaata gcagcataaa tagcagctga agcagaaatt
    13561 cctgcgagga aaccttctag tccgccgatg atacgtcctg tttcaatagc atcgttagat
    13621 gtaacgcgaa tgatatgatc ataagaatct gtagccaatg tttctggaat gaaacctgca
    13681 gaaatccctt gtattttatg aggcccaggt tgctcaccag aaaggattgc agattcatca
    13741 gcttctacag cataaatagc gacatcagga ttgacttttt ttaatgcacg agagacacca
    13801 gtaattgtcc caccagttcc cacacctgcg ataaaagcat ctaaaccttt ttcaccaaaa
    13861 gtctcgataa tttccctacc agtcgttttt tcgtggattt ctggattggc ttgattatta
    13921 aattgcaggg gtagccaagc attttgctct gcagaaattt cttttgcctt agcaatagct
    13981 cccttcatcc cttcactacc aggtgttagt actaattgag caccataggc ttgaatgatt
    14041 ttacgacgtt caattgacat ggtttctggc ataacaataa taacattata ccctttagct
    14101 tttcctaccc aagccaaacc gatacctgta ttaccagatg taggttctac gattgtatct
    14161 ccggctttaa gaatcccttt ttgctctgca tcttcaatca tagctagagc gatacgatct
    14221 ttgacagatg aacctgggtt aaaggattct aacttaacgt acacctctgc agctccttct
    14281 ggaacaatat gatgtaattg aataatgggt gtatttccga ttaagtctgt aatagaatta
    14341 taaatttttg acataagcta tacctcttca tgacattggt taagaatatt ataaaactca
    14401 cattgattaa agtaaaatat caattcttta tcaagtgata gctattattt atcggtttca
    14461 gccttaaagg gaatttcaac aatttgactc ccttcttcag ttaacaaagc ttttccttgg
    14521 tagaactctg tcaaattagt tattgtgtgt tcaaattcct tagggtcaac gtatatttta
    14581 caggttacag cttctaaaaa ttctgtttca aactcctgaa ggtggtgctc ttttaggaag
    14641 ttgtcaaaag tttgatattg aggataagtt aattgaatac gaataccaat ttgttcttta
    14701 acttctacta ccccgatctc tttgatggca ttagctacgc taccagagta ggcacgaata
    14761 agcccccctg tgcctaattt gatacctcca aaataccgtg taacgacggc aacaacattt
    14821 gtcaggcctt gcttttcaag tacggttagc atagggatac ctgctgtacc actaggttca
    14881 ccatcatcac tagatcgttt tatatcccct ttttcgccaa taaccatagc tgaacacgaa
    14941 tgattggctt tgtagtgcgc tttcttgatt tgcgtaatat aattacgccc ctcctcttcg
    15001 gactctactc gctttagatg acaaataaat cgcgattttt taatttcttc ttcgacaatt
    15061 ccgtctgatt ttatcgtttt atagttcata gagaaatttt acaaaaattc ttcaaattta
    15121 gcaaaaaaat ccgaatgaaa taatatggaa aattatttag gtagactttg gacaaaagca
    15181 caattatctg agcaacttag aaaaattgca attaacttgc catcttttat aaagaaagga
    15241 agcgactata tttgtactcg atgctctagt tctgttgcca aaaattgcca attaccaact
    15301 ggaaattatt attgtagaga atgtatagtt tttggaagag ttactagtaa tgaaaattta
    15361 tattatttcc cacaaaaaac attttctaaa acgaattctt taaaatggaa agggggactg
    15421 acaccttatc aaaatgaggt ttcagaagag ttgttgaaag gtatttcaag taaggaaaat
    15481 cttcttgttc atgctgtgac aggggctggt aaaacagaaa tgatatacca ctctatcgcc
    15541 aaagtaattg atactggagg gagcgtttgt attgccagtc ctagaattga tgtttgttta
    15601 gaattgcata agcgactttc aaacgatttt agatgtgcca taacattaat gcatggagaa
    15661 tctccttctt atcagagatc ccctttaact attgccacaa cacatcaatt gttaaaattt
    15721 tatcatgctt ttgatttgct aattgttgat gaagttgatg cttttccata tgtcgacaac
    15781 ccgatactat accatggtgt aaaacaggcc ttaaagaaaa acggaacaag tatttttctg
    15841 actgcaactt caacgactga gttagaaaga aaagttgcaa gaaaagaact aaaaaaatta
    15901 catctagcta ggagatttca tgccaatccg ttagtgatac ccgagatggt ttgggtaagt
    15961 ggtatccaga aaagcttgca gacacaaaaa ctacctccca aattatatca gctgataaat
    16021 aaacagcgac aaacgagata tcctttgtta ttatttttcc cccatattgc agaaggtcag
    16081 gtattcaccg aaatgttaag acaagccttt cctatggaaa aaattggttt tgtttcaagt
    16141 aagagcacat ctaggttaaa gttagtgcaa gattttcgag ataacaaatt gtctatccta
    16201 gtatcaacta ccattctaga gagaggagta actttcccta gcgtcgacgt ttttgttata
    16261 caagcaaatc atcatttgtt tacgaaaagt tccttggtac aaatttcagg aagagttgga
    16321 agagcattag aaagacccga agggctcctt tacttccttc atgatgggaa aagtaaatcg
    16381 atgcaccaag ccataaagga gattaaaaac atgaatcata taggaggatt ttaatgactt
    16441 gtttgttatg tcatgaaata gatttatcgc aattaacttt tgtggaactt atacttctaa
    16501 aaccaaagca aaacgtaatt tgtcaaacgt gcaagggtag ttttgaagca cttagtaggg
    16561 agatgggttg ccaaacatgt tgcaagcaaa tcctccgaaa acaatgtcaa gattgtattt
    16621 attggggtaa aaaaggtata gaggtaaatc atttttccct ttatagatac aatgaagcaa
    16681 tgaaaaaaat atttttctct ttttaaattt caaggggatt atttgttgaa agatgttttt
    16741 acaaaagaaa taaaggctgc tctaaaaagg tataagggct acactatagt gccagttccc
    16801 ttgagtcatg aaggatacca aaataggcaa tttaatcaag tgattgcttt tctacaatcg
    16861 gcaaatatac cttataaaaa tattctttct aaaaaagatg gaggtaagca atcggctaat
    16921 aataaagaag aaagactcaa acaagttcag caatttacat taaaaaatga ggctgagtta
    16981 agggataacc ttttaattgt tgatgatatt tatacaacag gcgcaacaat agcacaaatc
    17041 aggaaactat tagaagaaaa aggtataaaa aatataaaaa gtttttcatt agcacgctaa
    17101 aatcttgcaa attttcatga aagcggttat aataagaata tagaaagcgt taacaacgca
    17161 agaaagaggt actcatatga ttaaatacag tattcgtggt gaaaacatcg aagtaacaga
    17221 agctattcgt gagtatgttg agaccaaact ctctaaagtt gaaaaatact ttaatgaaga
    17281 acaagaatta gatactagag tcaacctcaa agtatacaga gagaagaccg caaaagttga
    17341 agtaacaatc ttaatagatt ctattacttt acgagcagaa gatgtttcac aagatatgta
    17401 tgggtcaatt gacttagttg ttgataaaat tgagcgtcaa attcgtaaaa ataaaactaa
    17461 aattgcgaaa aaatatcgtg aaaaaattcc agcaagtcaa gttttcacaa cagaatttga
    17521 ggctgagcca gatgaagaag cagtaagcca aagaattgta cgtactaaga atgttaatct
    17581 aaaaccaatg gatgttgagg aagcattgtt gcaaatggaa cttttaggtc atgatttctt
    17641 tatctataca gatgcagaag ataatacgac aaatgtcctt tacaagcgtg aagatggcga
    17701 actaggttta attgaagcta aaggaaatta actttgtaac ataatttaaa aaatctcaga
    17761 gttttttctg agattttttt a
//
LOCUS       DATRUK010000039        17510 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.039, whole genome shotgun sequence.
ACCESSION   DATRUK010000039 DATRUK010000000
VERSION     DATRUK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17510
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.039"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(23..1804)
                     /locus_tag="VBE34_001293"
     CDS             complement(23..1804)
                     /locus_tag="VBE34_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEO7264011.1"
                     /translation="MLRALKEDFFLTLTSLKNQFWVYIGTGILIQTLVAYLVKGILSF
                     IFRRILILSNTPAVTKDNWTLLFNHPLALLLFILYIVILIGFIYTEFAIYTIAILKTE
                     FSLSIKKWLTIFPKKIKSLLGPQLIWVSIYLLLTIPLANLGLRSSILEHLKIPDFISG
                     ELTKTVFGKIGYSTLLLLICYLNLRLIYFLPLTILTDYNAKEALLESWKLSRGKRQWR
                     LLSKIILTSLFISVIGTVALAVVAGTFSFIDHSGNNFPLQATFYNLLKSILFAVTLLI
                     KCLIISHLLADIKQNKSIVQQWRAWKGVHHKQKFKHIACVILALAIGTTALKNAIALA
                     LLQDNISIKKELIAHRSDTSHGVENSIEALKAAHKAKADYSEMDVIMTKDHKLVVIHD
                     DNLKRLSGMNKDVSKLTLDQVTKIPIHQGYFASYIPSLAEFMKTAQSLDQKIMIELKP
                     YNQNLDIYADEFIKEFKELRLSTKHKVMSLNLTLIEKVEKKLPQLDTGYLIPLHWGTL
                     QNHNVDFYGIEEFSYNDWIAYLAQEYNKQLYVWTINRDNLMIRYLQSPVNGIITDKLN
                     LFKVINKDIKNSPNYYQRALQLIDSEG"
     gene            1974..2576
                     /locus_tag="VBE34_001294"
     CDS             1974..2576
                     /locus_tag="VBE34_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO7264012.1"
                     /translation="MKFLELNKKRHAVKHFNDKPVDFKDVRTAIEIATLAPSANNIQP
                     WKFVVVQEKKSALAEGLPESNCNQINQAQYVIALFTDTDLGQRSRKIARIGRRSLPDD
                     LIGYYMETLPPRYALYSEKQTGEYLSLNAGIVAMNLVLALTDQGISSNMILGFDKAIT
                     NDVLEIDKRFRPEILITVGYSDEKVEPSYRLPVDHIIEKR"
     gene            2623..4029
                     /gene="pepV"
                     /locus_tag="VBE34_001295"
     CDS             2623..4029
                     /gene="pepV"
                     /locus_tag="VBE34_001295"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEO7264013.1"
                     /translation="MTIDFRAEVDKRKDALMDDLINLLRINSERDDSQADAEHPFGPG
                     PVKALEFFLEMAERDGYETKNVDNYAGHFTFGQGEEELGIFGHLDVVPAGSGWDTDPY
                     EPVIKDNRLYARGSSDDKGPTMACYYALKIIKELGLPTSKKVRFVVGTDEESGWGDMD
                     YYFEHVGLPKPDFGFSPDAEFPIINGEKGNITEYLHFSGENKGAVRLHSFSGGLRENM
                     VPESATARFTSHLDQTTLGASLADFAGKHNLKAELSVEDGQYIATVYGKSAHGSIPQE
                     GVNGATYLALYLSQFDFEGPARAFLDVTANIIHEDFSGEKLGVAYEDDCMGSLSMNAG
                     VFQFDETNDDNTIALNFRYPQGTDAKTIQTKLEKLNGVEKVTLSDHEHTPHYVPMDDE
                     LVSTLLAVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPGDENTMHQANEYMPLE
                     NIFRSAAIYAEAIYELIK"
     gene            4126..4710
                     /locus_tag="VBE34_001296"
     CDS             4126..4710
                     /locus_tag="VBE34_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEO7264014.1"
                     /translation="MTDLEKIIKAIKSDSQNQNYTENGIDPLFAAPKTARINIVGQAP
                     GLKTQEAILYWKDKSGDRLRQWLGVDEETFYHSGKFAVLPLDFYYPGKGKSGDLPPRK
                     GFAEKWHPLILKEMPNVQLTLLVGQYAQKYYLGSSAHKNLTETVKSYKDYLPDYLPLV
                     HPSPRNQIWLKKNPWFEKDLIVDLQKIVADILKD"
     gene            4725..5459
                     /locus_tag="VBE34_001297"
     CDS             4725..5459
                     /locus_tag="VBE34_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264015.1"
                     /translation="MRDNHLHTHFSYDCQTAFEDYINGFTGEFITTEHFDLSNPYTGQ
                     DDVPDYSAYCQKIDYLNQKYGNRFKKGIEIGYFKDRESDILDYLKNKEFDLKLLSIHH
                     NGRYDYLQEEALKVPTKELLADYFNRMEFAIGRVEAHVLDHFDYGFRKLNLDVEDLKP
                     FETQLKRIFRKMLSKGLAFELNTKSLYLYGNEKLYRYALEILKQLGCKQYSIGSDGHI
                     PEHFCYEFDRLQCLLKDYQIDENHLI"
     gene            5628..9386
                     /locus_tag="VBE34_001298"
     CDS             5628..9386
                     /locus_tag="VBE34_001298"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEO7264016.1"
                     /translation="MKRKDLFGDKQTQYTIRKLSVGVASVATGVCIFLHSPQVFAEEV
                     SVSPATTAIAKSNINQVDNRQSTNLKDDINSNSETVVTPSDMPDTKQLVSDETDTQKG
                     VTEPDKATSLLEENKGPVSDKNTLDLKVAPSTLQNTPDKTSQAIGAPSPTLKVANQAP
                     QIENGYFRLHLKELPQGHPVESTGLWIWGDVDQPSSNWPNGAIPMTNAKKDDYGYYVD
                     FKLSEKQRKQISFLINNKAGTNLSGDHHIPLLRPEMNQVWIDEKYGIHTYQPLKEGYV
                     RINYLSSSGNYDHLSAWLFKDVATPSTTWPDGSNFVNQGLYGRYIDVPLKTNAKEIGF
                     LILDESKTGDAVKVQPNDYVFRDLANHNQIFVKDKDPKVYNNPYYIDQVQLKDAQQTD
                     LTSIQASFTTLDGVDKTEILKELKVTDKNQNAIQISDITLDTSKSLLIIKGDFNPKQG
                     HFNISYNGNNVTTRQSWEFKDQLYAYSGNLGAVLNQDGSKVEASLWSPSADSVTMIIY
                     DKDNQNRVVATTPLVKNNKGVWQTILDTKLGIKNYTGYYYLYEIKRGKDKVKILDPYA
                     KSLAEWDSNTVNDDIKTAKAAFVNPSQLGPQNLSFAKIANFKGRQDAVIYEAHVRDFT
                     SDQSLDGKLKNQLGTFAAFSEKLDYLQKLGVTHIQLLPVLSYFYVNEMDKSRSTAYTS
                     SDNNYNWGYDPQSYFALSGMYSEKPKDPSARIAELKQLIHDIHKRGMGVILDVVYNHT
                     AKTYLFEDIEPNYYHFMNEDGSPRESFGGGRLGTTHAMSRRVLVDSIKYLTSEFKVDG
                     FRFDMMGDHDAAAIELAYKEAKAINPNMIMIGEGWRTFQGDQGKPVKPADQDWMKSTD
                     TVGVFSDDIRNSLKSGFPNEGTPAFITGGPQSLQGIFKNIKAQPGNFEADSPGDVVQY
                     IAAHDNLTLHDVIAKSINKDPKVAEEDIHRRLRLGNVMILTSQGTAFIHSGQEYGRTK
                     RLLNPDYMTKVSDDKLPNKATLIEAVKEYPYFIHDSYDSSDAINHFDWAAATDNNKHP
                     ISTKTQAYTAGLITLRRSTDAFRKLSKAEIDREVSLITEVGQGDIKEKDLVIAYQTID
                     SKGDIYAVFVNADSKARNVLLGEKYKHLLKGQVIVDADQAGIKPISTPRGVHFEKDSL
                     LIDPLTAIVIKVGKVAPSPKEELQADYPKTQSFKGSKTVEKVNRIANKTSITPVVSNK
                     TDSYLTNEANLPKTGDKSSKILSVVGISILASLLALLGLSLKRNRHLK"
     gene            9521..10439
                     /locus_tag="VBE34_001299"
                     /pseudo
     CDS             9521..10439
                     /locus_tag="VBE34_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
     gene            complement(10481..10657)
                     /locus_tag="VBE34_001300"
     CDS             complement(10481..10657)
                     /locus_tag="VBE34_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEO7264017.1"
                     /translation="MVKKFAFAKGIATGVVATAATLAGAAFAIKKTIIEPEEEKIAFI
                     EENRKKAARKRVSH"
     gene            10768..11658
                     /gene="miaA"
                     /locus_tag="VBE34_001301"
     CDS             10768..11658
                     /gene="miaA"
                     /locus_tag="VBE34_001301"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEO7264018.1"
                     /translation="MRKIKLIAVVGPTAVGKTALGIELAKTFNGEIISGDSQQVYQKL
                     DIGTAKASKEEQEQAYHHLIDVREVNENYSVYDFVKEAKVAIDTIISKGKIPIIVGGT
                     GLYLQSLFEGYHLGGEVNQETLKAYREKLESLSDEDLFEKLTEQSIVIPQVNRRRAIR
                     ALELAKFGNDLQNSESPYDVLLIGLNDDRQVLYDRINRRVDLMIDNGLLDEAKWLYDN
                     YPSVQASRGIGYKELFPYFSKQIPLEEAVDKLKQNTRRFAKRQLTWFRNRMNVEFIMV
                     GEENYQQKIKRKVSDFLSSK"
     gene            11749..12987
                     /gene="hflX"
                     /locus_tag="VBE34_001302"
     CDS             11749..12987
                     /gene="hflX"
                     /locus_tag="VBE34_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEO7264019.1"
                     /translation="MIETKEEQERVILVGVELQDTENFEMSMEELASLAKTAGANVVN
                     HYYQKRDKYDSKSFIGSGKLEEIKAIVEADEIDTVVVNNRLTPRQNSNLEAELGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQISDIERQLKIVEKNRETVRERRVDSTTFKIGLIGYTNAGKSTIMNVL
                     TDDKQYEANELFATLDATTKQIYLQNQFQVTLTDTVGFIQDLPTELVAAFKSTLEESR
                     HVDLLLHVIDASDPNHEEHEKVVMEILKDLDMIDIPRLAIYNKMDVTEQLNATTFPNV
                     RIAAKKQGSRDLLRRLIVDEIRHIFDEFSIRVHQNQAYKLYDLNKIALLDTYTFEEEY
                     ENITGYISPKQKWKLEEFYD"
     gene            12980..13627
                     /locus_tag="VBE34_001303"
     CDS             12980..13627
                     /locus_tag="VBE34_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEO7264020.1"
                     /translation="MIDYIDLALTYGGFTSLDKVYLEKKLDGLSKQQRLDFITPPPSV
                     INAYFAEIYQKQGPEAATDYYFDLSKALGLFPKHLSFDEEKPFIRLNLSGKSFGFAYL
                     NDQEEASVFSEVKEVITPQLLLEIAQIFPQYKVYQDRSGIRMAKIDFDETESQNITPE
                     TSLLGNVLQLKKDIIKITSFNQEELLELVKTKSGEYYYSSQGRESVIYIDTKSTN"
     gene            13669..14598
                     /locus_tag="VBE34_001304"
     CDS             13669..14598
                     /locus_tag="VBE34_001304"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEO7264021.1"
                     /translation="MEIQFLGTGAGQPAKARNVSSLVLKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKVKKIFITHMHGDHVFGLPGFLSSRAFQANEEQTDLDIYGPVGIKSFV
                     MTGLRTSGSRLPYRIHFHEFDESSLGKIMETDKFTVYAEKLDHTIFCMGYRVVQKDLE
                     GTLDAEALKLAGVPFGPLFGKVKNGENVTLEDGREIIAKDYISEPKKGKVITILGDTR
                     KTDASIRLALGADVLVHESTYGKGDERIAKSHGHSTNMQAADIAKQANAKRLLLNHVS
                     ARFMGRDCWQMEEDAKTIFSNTHLVRDLEEVGI"
     gene            14600..15361
                     /locus_tag="VBE34_001305"
     CDS             14600..15361
                     /locus_tag="VBE34_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO7264022.1"
                     /translation="MARTILITGASGGLAQAIINQLPQDDHLIVTGRSREKLEKLYGK
                     RPNTLCLSLDITNDNAVTNMIEKIYVEFGQIDILINNAGFGSFKEFWDYSDEEVKDMF
                     AVNTFATMSIARQIGHKMSLVKSGHIVNIASMAGLIATSKASVYGASKFAVVGFLNAL
                     RLELAEKNVYVTSVNPGPIKTGFFAQADPSGDYLASIGRFVLTPEKVAKKVVSILGKN
                     KRELNLPFILAFAHKYYSLFPKTADYFARKVFNYK"
     gene            15358..>17510
                     /gene="recJ"
                     /locus_tag="VBE34_001306"
     CDS             15358..>17510
                     /gene="recJ"
                     /locus_tag="VBE34_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEO7264023.1"
                     /translation="MISAKYSWVLNNQKPDAGFFEASKKEKISEAVASLIYSRGIKTS
                     AELHHFLQTNLENLHDPYLLNDMDKAVNRIRRAIENNETILVYGDYDADGMTSASIMK
                     EALDMMGAEVQVYLPNRFTDGYGPNQSVYKYFIEQQDVSLIITVDNGVAGHEAIAYAQ
                     NQGVDVVVTDHHSMPADLPCAYAIIHPEHPDANYPFPYLAGCGVAFKVACALLETIPT
                     EMLDLVAIGTIADMVSLTDENRIMVKAGLEVMKDSERIGLQELISLSNIDLKTLNEET
                     IGFKIAPQLNALGRLDDPNPAIELLTGFDDEESQAIAQMIDQKNEERKEIVQTIFDQA
                     MQMLDQTKPVQVLAKENWHPGVLGIVAGRILERTGQPVIVLNIEDGIAKGSARSVEAL
                     DIFQAFDQHRELFIAFGGHSGAAGMTLEESKVGDLSQVLCDYISKKQLDMSQKKTLTI
                     DSELRFDELSLDTVRDFEKLAPFGMDNKKPVFLLKDFKVSQARVMGQNGAHLKLKLEQ
                     DGQALDLVAFNMGSQLQEFQQAQHLELAVTLSVNQWNGATTLQLMLEDARVDGIQLFD
                     IRSKASSLPHGVPILSQEEQSKEVILLTVPDHPQELKQMTQGKQFDAIYFKNEIPKNY
                     FISGYGTRDQFASLYKTIYQFPEFDVRYKLKELSSYLHIPDILLIKMIQIFEELHFVT
                     ITEGIMTVNKEAEKRDISESQIYQELKETVKFQEL"
BASE COUNT         5934 a         2664 c         3410 g         5502 t
ORIGIN      
        1 tctactaagc tttttattta atttaaccct cgctatcaat aagttggaga gctctttgat
       61 aataattagg gctatttttg atatctttat taatgacctt gaaaagattg agtttatctg
      121 tgatgattcc attaactggg ctttgtaaat aacgtatcat aaggttatca cggttaatag
      181 tccaaacata gagctgttta ttatattctt gggcgagata tgcaatccaa tcattgtatg
      241 aaaattcctc aatcccataa aagtcaacgt tatgattttg aagcgttccc caatgtagtg
      301 ggattaggta acctgtatca agttgtggca attttttctc aaccttttca atgagtgtca
      361 agttgagaga cattacttta tgcttggttg atagacgaag ctctttaaat tctttgataa
      421 attcatccgc atagatatcg aggttttgat tatacggttt caattcaatc atgatttttt
      481 gatctagtga ctgagcggtc ttcataaatt ctgccaatga tggaatgtaa ctagcaaaat
      541 acccttggtg aattgggatt tttgtaactt gatctaaagt tagtttagaa acatctttgt
      601 tcataccaga gagtcgtttg agattatcat catgaatgac tactaattta tgatcttttg
      661 tcatgataac atccatctct gaataatctg cttttgcttt atgggctgct tttaatgctt
      721 caatagaatt ttcaacgcca tgtgatgtat cactacgatg ggctattaat tcttttttaa
      781 ttgaaatgtt atcttgtaag agagccagag ctatagcatt ttttaaggca gtggtaccaa
      841 tagctagagc tagaataaca caagcaatgt gtttaaattt ttgcttatgg tggacgcctt
      901 tccaagctct ccattgttga acaatacttt tattttgttt aatatctgct aataggtgac
      961 ttatgattaa acatttaata agaagtgtta cggcaaaaag tattgatttt aaaaggttat
     1021 agaaagtggc ttgaagagga aaattgtttc cagaatgatc aatgaatgaa aatgtaccag
     1081 caacaacagc tagtgcaact gttccgatta cagatataaa taaactggtt aaaataattt
     1141 tacttagaag acgccactga cgctttccgc ggctaagttt ccaactctct aaaagtgctt
     1201 ctttagcatt gtagtcagtt aaaatggtca gcgggagaaa ataaattaat cgaaggttta
     1261 agtaacaaat aagcagtaaa agggtagagt aacctatctt tccaaagact gtctttgtta
     1321 actcgccaga aataaagtca ggaattttaa gatgctctaa tatacttgac cttagaccaa
     1381 ggttagctag aggaattgtc aaaagtaagt agattgatac ccaaattagc tgtggtccta
     1441 aaagtgattt tattttttta ggaaagattg ttagccattt tttgatactg agagaaaatt
     1501 cagttttcag tatggcaata gtatagatag cgaactcagt atagatgaac ccaattagga
     1561 ttacgatata aagtataaag agtaataaag ctaagggatg attaaaaagc aaagtccaat
     1621 tgtctttagt cactgcggga gtatttgata atatcaatat ccgacgaaag ataaaactca
     1681 gaattccttt tactaaataa gcaactaaag tttggattaa gataccagtt ccaatgtaaa
     1741 cccaaaattg attttttaaa gaggttaagg ttagaaaaaa atcttctttt aatgctcgta
     1801 gcatgaatag ctcctttttt atagtcatta gatattgtac cacaagtttg tttgtttttt
     1861 atggtagttt tcaaaccggt ttcacttttt tatcaatttt gccttcaaaa gctgaaacgc
     1921 attttaaaaa gtgatagaat agtagggaaa atcaaataga ataggaggtt tttatgaaat
     1981 ttctagaatt aaataagaag cgccatgctg tgaaacattt taatgataag ccagtcgact
     2041 ttaaagatgt tagaactgct attgaaattg cgacattagc gccatctgct aataatattc
     2101 agccttggaa gtttgttgtt gtccaagaaa aaaaatctgc tctagcagaa ggtttaccgg
     2161 aaagtaattg taaccaaatt aatcaagcgc agtatgttat tgctttattt acagatacag
     2221 atttaggaca gagatcacgg aaaatagctc gtattggtcg tagatctctt cctgatgatt
     2281 taattggtta ttatatggaa actctaccac cacgctatgc tttatatagt gaaaaacaaa
     2341 ctggagagta tctctcttta aatgcaggta tcgttgctat gaatttagta cttgccttga
     2401 cagatcaagg tattagttct aatatgattt tagggttcga taaagcgatt accaatgatg
     2461 tcttagagat agataaacgt tttagaccag aaatattaat tactgttggt tatagtgatg
     2521 aaaaggttga gcctagttat cgtttgccag ttgatcatat tattgaaaaa cgttaatttt
     2581 taagcacaaa ctacataata ttttatagaa ggagttgccg taatgacaat tgattttaga
     2641 gcagaagtag acaaacgcaa agacgctttg atggacgatt taataaatct tcttcgtatt
     2701 aactcggagc gtgatgattc acaagctgat gcagagcatc catttggtcc tggacctgta
     2761 aaagccttgg aatttttcct tgagatggct gagcgagatg gctacgaaac aaaaaacgta
     2821 gataattatg cgggacactt tacatttggt caaggcgaag aagaactagg tatttttggg
     2881 catttagatg ttgttcctgc aggcagcggt tgggatacag acccttatga accagttatc
     2941 aaggataacc gtttatatgc tcgagggtca tcagacgata agggaccaac aatggcatgt
     3001 tattatgctc taaaaattat taaagagctt gggctaccaa catctaagaa agttcgtttt
     3061 gttgtaggaa ctgatgaaga atctggatgg ggagatatgg attactactt cgaacatgtt
     3121 ggtcttccaa aacctgattt tggtttctct ccagatgcag aatttccaat tataaatggt
     3181 gaaaaaggta atatcactga atatttacat ttttcaggag aaaataaggg agctgttaga
     3241 ctgcatagct tttctggcgg acttcgcgaa aacatggttc cagaatcagc cacagcacgt
     3301 ttcacaagtc atttagatca gacaacactt ggagctagtc tagctgattt tgcaggtaaa
     3361 cataatttga aagcagaatt atctgtagaa gatgggcagt atatagctac agtttatggt
     3421 aaatctgctc atggttcaat accacaagaa ggtgttaatg gggcgactta tttagctctt
     3481 tatctaagtc aatttgattt tgaaggtcct gctcgtgctt tcttagatgt cacagccaac
     3541 attattcacg aagacttctc aggtgaaaaa cttggagtag cttatgaaga tgactgtatg
     3601 ggatcgttga gcatgaatgc aggtgtcttc cagtttgatg aaactaatga tgataatact
     3661 atcgctctta atttccgtta cccacaaggg acagatgcta aaactatcca aactaagctt
     3721 gagaaactta acggagttga aaaagtgact ctttctgacc atgaacacac accacactat
     3781 gtacctatgg acgatgaatt agtatcaacc ttactagctg tctatgaaaa gcaaactggt
     3841 cttaaaggac atgaacaggt tattggtggg gggacatttg gtcgcttact tgaacggggt
     3901 gttgcatacg gtgccatgtt cccaggagat gaaaacacta tgcatcaagc taatgagtac
     3961 atgcctttag aaaatatttt ccgttcggct gctatctacg cagaagctat ctatgaatta
     4021 atcaaataaa ataatcctta aactaaatat gtgatcaatg ataaagggtg gtgaagacat
     4081 gaaagtgtct ttgcctcttt tcataaggtt agatttggag actttatgac tgacttggaa
     4141 aaaattatta aagcaataaa aagtgattca cagaatcaaa attatacaga aaatggtatt
     4201 gatcctttgt ttgctgctcc taaaacagct aggatcaata ttgttggaca agcacctggt
     4261 ttaaaaactc aagaagcaat actctattgg aaagataaat ctggagatcg tctacgccag
     4321 tggcttggag ttgatgaaga gacattttac cattctggaa aatttgctgt tttgccttta
     4381 gatttttatt acccaggcaa aggaaaatca ggagatttac cccctagaaa aggttttgcg
     4441 gagaaatggc accctcttat tttaaaagaa atgcctaatg ttcaattgac cttgctagtt
     4501 ggtcaatatg ctcagaaata ttatcttgga agctccgcac ataaaaatct aacagaaaca
     4561 gttaaatctt acaaagacta tctacccgat tatttacccc tggttcaccc atcaccgcga
     4621 aatcaaattt ggctaaagaa gaatccatgg tttgaaaaag atctaatcgt tgatttacaa
     4681 aagatagtag cagatatttt aaaagattaa ggataggagt tagtatgaga gataatcatc
     4741 tacacacgca tttttcctat gattgtcaaa cggcatttga ggactatatt aatggtttta
     4801 caggtgaatt tatcacaaca gaacattttg atttatcaaa tccttacacc ggtcaagacg
     4861 atgttcctga ttatagtgct tattgtcaaa aaatagatta tcttaatcag aaatatggaa
     4921 atcgatttaa aaaaggaatt gaaatcggtt attttaaaga tagggaatca gatattttag
     4981 attatttaaa aaataaagaa tttgatttaa aactattgtc aatccatcat aatggtaggt
     5041 atgattatct gcaagaagaa gctctgaaag taccaacaaa ggagctttta gcagattact
     5101 ttaatcgtat ggaatttgcc ataggccgtg tggaagcgca cgttttagat cactttgatt
     5161 atggttttcg taagttaaac ttagatgtag aagatttaaa accgtttgaa acgcaattga
     5221 agcgcatttt cagaaagatg ttatctaagg ggttagcttt tgaactaaat accaaatccc
     5281 tttatctata tgggaatgaa aaactttatc gctatgcttt agagatacta aaacagcttg
     5341 gttgtaaaca atactctata ggctctgacg gtcatattcc tgaacatttt tgttatgaat
     5401 ttgatagact tcaatgtctg ctaaaggact atcaaattga tgaaaatcat ttgatatgag
     5461 gaaatttttg ataaaaaagc taggcaatat tgcttagctt ttttgtaatg tcattgatag
     5521 ttttagtgaa aatttcaaaa aaataaagaa atcacttact tgttgcaagc gcttgcgtaa
     5581 attgttatga ttttattggt aacaattcat taaaaaagga gaatgatatg aaaagaaaag
     5641 acttatttgg tgataaacaa actcaataca cgattagaaa gttaagtgtt ggagtagctt
     5701 cagttgcaac aggggtatgt atttttcttc atagtccaca ggtatttgct gaagaagtaa
     5761 gtgtttctcc tgcaactaca gcgattgcaa agtcgaatat taatcaggtt gacaaccgac
     5821 aatctactaa tttaaaagat gacataaact caaactctga gacggttgtg acaccctcag
     5881 atatgccgga taccaagcaa ttagtatcag atgaaactga cactcaaaaa ggagtgacag
     5941 agccggataa ggcgacaagc ctgcttgaag aaaataaagg tcctgtttca gataaaaata
     6001 ccttagattt aaaagtggca ccatctacat tgcaaaatac tcccgacaaa acttctcaag
     6061 ctataggtgc tccaagtccg accttgaaag ttgctaatca agctccacag attgaaaatg
     6121 gttactttag gttacatctt aaagaattgc cacaaggtca tcctgtagaa agcactgggc
     6181 tttggatatg gggagatgtt gatcaaccgt ctagtaattg gccaaatggt gctatcccta
     6241 tgactaatgc taagaaagat gattacggtt attatgttga ttttaaatta tctgaaaaac
     6301 aacgaaaaca aatatctttt ttaattaata acaaagcagg aacaaattta agcggcgatc
     6361 atcatattcc attattacga cctgagatga accaagtttg gattgatgaa aagtacggta
     6421 tacatactta tcagcccctc aaagaagggt atgtccgtat taactatttg agttcatctg
     6481 gtaactatga ccacttatca gcatggctct ttaaagatgt tgcaaccccc tcaacaactt
     6541 ggccagatgg tagtaatttt gtgaatcaag gactatatgg aaggtatatt gatgtaccac
     6601 tgaaaactaa tgccaaagag attggttttc taatcttaga tgaaagtaag acaggagatg
     6661 cagtgaaagt tcaacccaac gactatgttt ttagagattt agctaaccat aaccaaattt
     6721 ttgtaaaaga taaggatcca aaggtttata ataatcctta ttacattgat caagtgcagc
     6781 taaaggatgc tcaacaaact gatttaacaa gtattcaagc aagttttaca actctagatg
     6841 gggtagataa aactgaaatt ttaaaagaat tgaaagtgac agataaaaat caaaatgcta
     6901 tacaaatttc tgatatcact ctcgatacta gtaaatctct tttaataatc aaaggcgact
     6961 ttaatcctaa acaaggtcat ttcaatatat cttataatgg taacaatgtc acgacaaggc
     7021 aatcttggga atttaaagac caactttatg cttatagtgg aaatttaggt gcagttctca
     7081 atcaagatgg ttcaaaagtt gaagccagcc tctggtcacc gagtgctgat agtgtcacta
     7141 tgattattta tgacaaagat aatcaaaaca gggttgtagc gactaccccc cttgtgaaaa
     7201 ataataaagg tgtttggcag acgatacttg atactaaatt aggtattaaa aactatactg
     7261 gttactatta tctttacgaa ataaaaagag gtaaggataa ggttaagatt ttagatcctt
     7321 atgcaaagtc attagcagag tgggatagta atactgttaa tgacgatata aaaacggcta
     7381 aagcagcttt tgtaaatcca agtcaacttg gacctcaaaa tttaagtttt gctaaaattg
     7441 ctaattttaa aggaagacaa gatgctgtta tatacgaagc acatgtaaga gacttcactt
     7501 ctgatcaatc tttggacgga aaattaaaaa atcaacttgg tacctttgca gccttttcag
     7561 agaaactaga ttatttacag aaattaggag ttacacacat tcagctttta ccggtattga
     7621 gttattttta tgttaatgaa atggataagt cacgctcaac agcttacact tcctcagaca
     7681 ataattacaa ttggggctat gacccacaga gctattttgc tctttctgga atgtattcag
     7741 agaaaccaaa agatccatca gcacgtatcg ccgaattaaa acaattaata catgatattc
     7801 ataaacgtgg catgggggtt atacttgatg tcgtctataa tcacactgca aaaacttatc
     7861 tctttgagga tatagaacct aattattatc actttatgaa tgaagatggt tcaccaagag
     7921 aaagttttgg agggggacgt ttaggaacca ctcatgcaat gagtcgtcgt gttttggttg
     7981 attccattaa atatcttaca agtgaattta aagttgatgg tttccgtttt gatatgatgg
     8041 gagatcatga tgcggctgcg attgaattag cttataaaga agctaaagct attaatccta
     8101 atatgattat gattggtgag ggctggagaa cattccaagg cgatcaaggt aagccggtta
     8161 aaccagctga ccaagattgg atgaagtcaa ccgatacagt tggcgtcttt tcagatgata
     8221 ttcgtaatag cttgaaatct ggttttccaa atgaaggtac tccagctttc atcacaggtg
     8281 gcccacaatc tttacaaggt atttttaaaa atatcaaagc acaacctggg aattttgaag
     8341 cagattcgcc aggagatgtg gtgcagtata ttgctgcaca tgataacctt accttgcatg
     8401 atgtgattgc aaaatcaatt aataaagacc ctaaggtagc tgaagaagat attcatagac
     8461 gtctgcgttt aggaaatgta atgattttaa catctcaagg gacagcattc attcattctg
     8521 gtcaagagta tggtcgtacg aagcgtttac ttaaccctga ttacatgaca aaagtttcag
     8581 atgacaaatt gcctaataaa gcaacactta ttgaagctgt taaagaatac ccatatttta
     8641 ttcatgattc atatgattct tcagatgcca ttaatcattt tgattgggca gcagccacag
     8701 ataataacaa acacccaatt tcaacgaaaa cacaggccta tacagcaggt ttaatcacat
     8761 taaggcgttc aacagatgct ttccggaaat tgagcaaagc agaaattgat cgtgaggtta
     8821 gcttgattac agaggtaggt caaggtgata ttaaagaaaa agatttggtt attgcttacc
     8881 aaacaataga ttctaaaggc gatatttacg cagtatttgt taatgctgat agtaaagcta
     8941 gaaacgtttt actaggtgaa aaatataaac accttttaaa agggcaagta attgttgatg
     9001 ctgatcaagc ggggattaaa ccaatctcaa ctcctagagg tgttcatttt gaaaaagata
     9061 gtttgctgat tgatccatta acagcaattg tgattaaagt tggcaaagtt gctcctagcc
     9121 ctaaggagga attgcaagca gattatccca aaacacaatc tttcaaggga tctaaaacgg
     9181 tagaaaaagt aaatagaata gctaataaga cctcaataac tcctgtagtt tctaataaga
     9241 ccgattcata tctgacaaat gaagctaatt tgccaaaaac tggagataag tcatcaaaaa
     9301 tactaagtgt agtaggaata agcattctag caagtctact tgctctacta ggtctctctt
     9361 taaagaggaa tcgtcactta aaataaaagc aaactttaga agagcttaaa aaagaagtta
     9421 gtcaatgggc tagcttcttt tcattaagtt aataaaatca cttatcagca aaatctaatt
     9481 gtgttataat ggatgtaaac gtttttttat gaggtatatg atgaaaaatt gggaaaaacc
     9541 accattagtg atggcgggat tagtattggg gctattagct ttggggaacc ttctagaggg
     9601 atatggcacc tattttcgct attgtttagg gctagtagca cttgtttttt ggattttttt
     9661 aatcaaagga atattaaaaa ataaaaaaga aagcaggaag gagttaagca atcctttgat
     9721 tgcttctgtc tttactactt tttttatggc aggtatgatt ttatcgacct atatcttatt
     9781 atttagaagt ttaggaatat gggtagcagt tctgtcaaaa aggtgtatgg tggttgtcat
     9841 ttattgcgtt aatcattcat atggctattt tttcatggaa atatttgaga cacttttcga
     9901 tggctaatct ttttccttct tggtctgtcc tctatgttgg tataggggta gctagcctga
     9961 cagcaccaat tagtggacaa ttcactattg ggaaaatagt tttttggtat ggttttattg
    10021 caactttagt attattacca tttctattca ttaaagcata taaaatcggg ctaccttcgg
    10081 cagttaagcc taatataact actatttgcg ctccaatgtc gctaatcaca gcgggatatg
    10141 ttaatagttt tgtgtcacca aatagaggtt tattattgct attaatcgta atggcacaat
    10201 ttctttattt tttcattctt tttcaggtgc ctaaattact gattggtgat tttacaccag
    10261 gtttctcagc ctttactttc ccccttgtta tctcagctac ctcacttaaa ttaagtattc
    10321 agcatctatc attaccagtt gatatacagg ggctagtcca ctttgaaatt gggacaacaa
    10381 ctcttattgt tatgatagta atggtacgtt atattttctt tttaaggcga acaatttaat
    10441 ataaaataac tcccttacta gctagtaagg gagttattgt ttaatgagag acacgtttac
    10501 gtgcagcttt tttacgattt tcttcgataa aagctatttt ttcttcctca ggttcaataa
    10561 ttgttttttt gatagcaaat gcagcacctg caagagtagc agctgttgca acgactcctg
    10621 ttgctattcc tttagcaaat gcgaattttt taaccataat aattctccaa tctatgatga
    10681 agactctgct tctatctatg atataattat ggtaacgaaa tactagttaa atttcaagta
    10741 aaaaccttta tagaattgag aagtatcatg agaaaaatca agcttatagc agttgttggt
    10801 ccaactgcgg ttgggaagac agcattagga attgaattag ctaaaacttt caatggcgaa
    10861 atcattagtg gcgatagtca gcaagtttat cagaagttag atatcggaac ggctaaagct
    10921 agtaaagaag agcaagaaca agcttatcat cacttgattg atgttcgtga agtaaatgag
    10981 aattattcgg tttatgattt tgtcaaagag gcaaaagtag ctattgatac gattatttct
    11041 aaggggaaaa tccctattat tgttggaggt actggcctat atttacagag tttgtttgag
    11101 ggctatcatt taggaggcga agtcaatcaa gaaactctta aggcctatcg tgaaaaatta
    11161 gagtctctaa gtgatgaaga tttatttgaa aagttgacag aacaatccat tgttataccg
    11221 caagttaatc gtcgacgtgc catccgtgca ttagaattgg cgaagtttgg gaatgatttg
    11281 caaaatagtg aaagtccgta tgatgtttta ttaattggat tgaatgatga tcgtcaggtg
    11341 ctctatgatc gtatcaatag acgagttgat ttgatgatag acaatggtct cttagatgaa
    11401 gctaaatggc tatacgataa ctaccctagt gttcaagcaa gtagaggaat tggctataaa
    11461 gaattatttc cttacttttc aaagcaaata ccattagaag aagctgttga taaactaaaa
    11521 caaaatacca gacgttttgc aaaacgccaa ttaacatggt ttagaaaccg tatgaatgtc
    11581 gaatttatta tggttggtga ggaaaattat caacaaaaaa tcaagcgaaa agtcagtgat
    11641 tttttatcat caaagtaaat aaagccgttt ctcgcattta aagggcatta tgttataata
    11701 aagattacgt atttagaaca agaaaaaaat attgaaagga gattataggt gatagaaact
    11761 aaagaagagc aagaacgtgt tattttagtt ggggtagaac ttcaagatac tgagaatttt
    11821 gaaatgtcca tggaagaact tgctagctta gctaagacag caggagcaaa tgttgttaat
    11881 cattattatc aaaaaagaga taaatatgat agtaaatcat ttatcggatc tggtaaatta
    11941 gaggagataa aagctattgt agaagcagat gagattgata ccgttgtggt taataatcgt
    12001 ttgactccgc gacaaaactc taatttggaa gctgaacttg gtgttaaagt tattgaccgt
    12061 atgcaactta ttttagatat ctttgctatg cgtgctagaa gtcatgaagg aaaattacag
    12121 gttcatctag cgcaattgaa atatatgtta ccacgcttag ttggacaagg tattatgctt
    12181 agtcgtcaag caggaggaat cggaagccga ggtccaggag aatctcagtt agagttgaat
    12241 cgtcgttcca ttcgtcatca aatttctgat atcgaaagac aattgaagat tgtagaaaaa
    12301 aatcgcgaga cagttcgaga aagacgtgtt gattcgacaa cctttaaaat tggattaatt
    12361 ggatatacaa atgctggcaa gtcaactatt atgaatgttt tgaccgatga taagcaatat
    12421 gaagccaatg aattatttgc aacgttagac gctacaacca agcagatcta tttacaaaat
    12481 caatttcaag tcactttaac agatactgtt ggttttattc aagatttacc tacagagtta
    12541 gttgcggctt tcaagtctac cttagaagaa agtcgacatg ttgatttatt acttcatgtt
    12601 attgatgcta gtgaccctaa tcatgaagaa cacgaaaaag ttgttatgga gattcttaaa
    12661 gacttagata tgatagatat tcctcgttta gcgatataca acaagatgga tgtcactgag
    12721 cagctgaatg caacaacttt tcctaatgtg agaattgcag ccaaaaaaca agggagtagg
    12781 gatttattac gccgtcttat tgtggatgaa atacgacata tctttgacga attttcgatt
    12841 agagtgcatc aaaatcaagc atataagctc tatgatctca ataaaatagc attgcttgac
    12901 acctacacct ttgaagaaga atacgagaat attacaggct atatttctcc taaacaaaaa
    12961 tggaagctgg aagaatttta tgattgatta tattgatttg gctttgactt atggtggttt
    13021 tacgagctta gataaagttt atctagagaa aaaactagat ggcctctcta agcaacaacg
    13081 acttgatttt atcactccac ctcctagtgt gattaatgct tattttgctg aaatatatca
    13141 aaagcaaggg ccagaagcgg caactgatta ctattttgat ctttccaaag ccttgggctt
    13201 gtttccaaaa catttatcgt ttgatgaaga aaaacctttt attcgtttga atttatcagg
    13261 aaaatcattt ggttttgctt acctaaatga tcaagaagaa gcgagtgttt tttctgaagt
    13321 taaagaagtc attacaccgc agttactctt agaaattgca cagatttttc ctcaatataa
    13381 agtctatcaa gatcggtctg gtatcagaat ggcaaaaata gattttgacg aaacagaatc
    13441 tcaaaatatt acaccagaaa caagtttact agggaatgtc ttacaactta aaaaagatat
    13501 tattaaaata actagtttca atcaagaaga gctattagag ttggttaaaa caaagtcagg
    13561 tgaatactat tattctagtc aaggacgtga gtcagttatt tatattgata ctaaatctac
    13621 taattaaatt tattaagtta tggaaggatt ataaaaaagt caacaagtat ggaaattcaa
    13681 tttttaggaa ctggagctgg tcaaccagcg aaagctcgta atgtttcaag tttagtcttg
    13741 aaattattag atgaaattaa cgaggtttgg atgtttgatt gtggagaggg tacacagcgt
    13801 caaattttag aaaccacgat taaacctcgt aaagtcaaaa aaatttttat cacgcacatg
    13861 cacggagacc atgtttttgg tcttccaggt tttttatcca gtcgggcttt tcaagccaat
    13921 gaagaacaga cagatttaga tatttatgga ccggtcggta taaaaagttt tgttatgact
    13981 ggcttaagga catcaggttc acgtttacct tatcgaattc attttcacga gtttgatgaa
    14041 tcatcactcg gaaaaattat ggaaacagat aaatttaccg tttatgcaga aaagctagat
    14101 catactatct tttgtatggg atatcgtgtt gtgcaaaaag accttgaggg cactttagat
    14161 gctgaagcat tgaaactagc aggtgttcca tttggtcccc tttttgggaa agtcaaaaat
    14221 ggtgagaatg ttaccttaga agatggtaga gaaattattg ctaaagatta tatttctgaa
    14281 ccgaaaaagg gtaaagtaat cactattttg ggagatacta gaaaaacaga tgcaagtatt
    14341 aggttagctt tgggcgctga cgttttagtg cacgaatcta cttatggtaa gggtgatgag
    14401 cgaattgcta aatcgcatgg gcattcaacc aatatgcaag cagctgatat cgctaaacaa
    14461 gcaaatgcta aacgactatt attaaatcat gttagtgctc gttttatggg gcgagattgt
    14521 tggcaaatgg aagaagatgc aaagacgatt ttttcaaata cccatcttgt tagagatttg
    14581 gaagaagtag gtatataaaa tggcaagaac gatactgatt acaggagctt ctggtggttt
    14641 agcacaggca attattaacc aattacctca agacgaccat ttaattgtca cagggagatc
    14701 aagagaaaaa cttgaaaagc tttatggaaa gagaccaaac accttatgtt tatcactaga
    14761 cataactaat gacaatgctg taacgaatat gattgaaaaa atttatgttg aatttggtca
    14821 aattgatatt ttaattaata atgctggatt tggttctttt aaagaatttt gggattatag
    14881 tgatgaagaa gtcaaagaca tgtttgctgt gaatacattt gcaacgatgt ctatagctcg
    14941 tcagatagga cataagatgt ctcttgtaaa atctggtcat atcgttaata ttgcaagcat
    15001 ggctggcctc atagcaacaa gtaaagctag cgtttatggg gctagtaagt ttgctgtcgt
    15061 tggatttttg aatgctcttc gtttagaatt agcagaaaaa aatgtttatg tgacaagtgt
    15121 aaaccctgga ccaatcaaaa caggattttt tgctcaggct gatccaagtg gtgattattt
    15181 agcaagtata ggtcgttttg ttctaacacc agaaaaagta gctaaaaaag tagtttctat
    15241 tcttggaaaa aataagagag aattaaatct tccttttata ttagcttttg cgcataaata
    15301 ttatagttta tttccaaaaa cagctgatta ttttgccaga aaggtattca attataaatg
    15361 atatctgcta agtatagttg ggtcctcaat aaccaaaagc cagacgctgg cttttttgag
    15421 gcttcaaaaa aagaaaagat aagtgaagcg gtggcttcct taatttattc gcgaggtatt
    15481 aagacatcag cggaattaca tcatttttta caaactaatt tggaaaatct ccatgatcct
    15541 tatttgttaa atgatatgga taaagctgtt aatcgtatta gacgagctat tgaaaataat
    15601 gaaactattt tagtttatgg agactatgat gctgatggaa tgacatcagc atcaatcatg
    15661 aaagaagcat tagatatgat gggagctgaa gtccaggttt atctcccaaa tcgctttaca
    15721 gatggttacg gtcccaatca aagtgtttat aaatatttta ttgaacaaca agatgtttca
    15781 ttaatcatta cggttgataa tggtgtggca gggcatgaag caattgcata tgctcaaaat
    15841 caaggtgtgg acgtggtagt tactgatcat cacagtatgc cagcagattt accttgtgct
    15901 tatgctatta ttcacccaga acatcctgat gctaattatc catttcctta tttagcaggg
    15961 tgtggtgttg ctttcaaagt agcttgtgct ctcttagaaa caatcccgac agaaatgttg
    16021 gatttggttg ctattgggac gattgcagat atggttagcc taactgatga aaatcgtatc
    16081 atggtaaaag ctggacttga agttatgaaa gatagtgaaa gaataggctt acaggagcta
    16141 atatctttat caaatattga tttaaaaaca ctaaatgaag aaactattgg atttaaaatt
    16201 gctccacaat taaacgcttt aggaagactt gatgacccta atccagctat tgaattacta
    16261 acagggtttg atgatgaaga atctcaagca atagcccaaa tgattgatca aaaaaatgaa
    16321 gagcgcaaag aaatcgttca aactatcttt gaccaagcta tgcaaatgct tgatcaaaca
    16381 aagcctgtac aagttttagc aaaagaaaat tggcatccag gggttttagg tattgtggcg
    16441 ggtcgtattt tagagagaac tggacaacct gttattgttt tgaatattga agatggtatt
    16501 gctaaaggaa gtgccagaag tgtagaagca cttgatattt ttcaagcttt tgaccagcat
    16561 cgagaattat tcattgcttt tggaggtcac agcggagcag caggcatgac tttagaggaa
    16621 agtaaggtag gtgatctttc tcaagtttta tgtgactata tctctaaaaa acaattggac
    16681 atgagtcaga aaaaaactct gacaattgat agtgagctac gttttgacga acttagttta
    16741 gacactgtta gagattttga aaagttagcg ccatttggaa tggataataa aaaaccagtc
    16801 tttttgttaa aagattttaa agtgagtcaa gccagagtca tggggcaaaa cggtgctcat
    16861 ctgaaattaa aactagaaca agatggacaa gcacttgatt tggtagcatt taatatgggg
    16921 agtcaactac aagagtttca acaagcacaa catttggagt tagctgtcac tttatctgtt
    16981 aatcaatgga atggtgccac aaccctacaa ttaatgttgg aggatgcgcg tgtagatggt
    17041 attcaattat ttgatattag aagcaaagca agtagcttac ctcatggagt gccaatcctg
    17101 tctcaagaag agcagagcaa agaagttatt cttttgactg ttcccgatca tccacaagaa
    17161 ttaaaacaaa tgactcaagg taaacagttt gatgctatct attttaaaaa tgagatacca
    17221 aaaaattatt ttatatctgg ttatggtaca cgtgatcaat ttgctagttt atataaaaca
    17281 atatatcaat tcccagaatt tgacgttcgt tataagttaa aagagctcag ttcctacctt
    17341 catattcctg atattttatt aattaaaatg attcaaattt ttgaagaact acattttgtt
    17401 acgattacag aggggattat gacagtcaat aaagaagcag aaaagcgaga tatctctgag
    17461 agtcaaattt atcaagaatt aaaagagaca gttaagtttc aagaattaat
//
LOCUS       DATRUK010000040        17267 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.040, whole genome shotgun sequence.
ACCESSION   DATRUK010000040 DATRUK010000000
VERSION     DATRUK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17267
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.040"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            160..1404
                     /locus_tag="VBE34_001307"
     CDS             160..1404
                     /locus_tag="VBE34_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO7264024.1"
                     /translation="MKIVEGVSLHLIKNQQFKTNHLTFRFSGDFNNKTVARRSLVAQM
                     LVTANAKYPKVQEFREKLASLYGASLSTKISTKGLVHIVDIDIVFVKNTFTLEQENIV
                     EQIITFLEDMLFSPLISLEQYQTSIFDTEKKNLIQYLEADIEDNFYSSDLALKSLFYN
                     NKTLRLPKYGTASLVESENSFTAYQEFQKMLKEDQLDIFVVGDFDDYRMIQAFNRMAF
                     EPRHKVLAFDYTQTYENITRSQVEDKDVNQSIMQLAYHLPITYKDEDYFALIVFNGLF
                     GAFAHSLLFTEIREKQGLAYTIGSQFDSFTGLFTIYAGIDKENRERFLKLINKQFNNI
                     KMGRFSSTLLKQTKDILKMNYVLASDNPKVIVDHIYHEHYLDQFHTSALFIDKVDDVT
                     KSDIVSVATKLKLQAFYFLEGN"
     gene            1406..2689
                     /locus_tag="VBE34_001308"
     CDS             1406..2689
                     /locus_tag="VBE34_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO7264025.1"
                     /translation="MTVNKITYQNLQEEVYKLTLESGLNVYLIPKPSFKETVGVLTAN
                     FGSLHTRYTRNGCVEHYPAGIAHFLEHKLFELDKGQDAATQFTKYGAESNAFTTFDKT
                     SFYFSTISHITNCLDILLDFVLTTNFTEESITKEKDIIKQEIEMYQDDPEYRLYQGVL
                     SNLYPNSPLAFDIAGDYQSISQITLTDLQENHKDFYQLSNMNLVLVGQFSPQEIITYL
                     QKNSHLTNYSQNIDRDSISLEPVIKNNSCHMTVTKPKLAIGYRKSSHMRHGSYLKEKI
                     GLQLFFAMLLGWTSTINQDWYESGQIDDSFDIEIEVHPDFECVIISLDTTEPIAFSTQ
                     LRLLLKNALQSSDLTESHLQNVKRELYGDFLRSLDSIENLAMQFVTYLYDGKTMYLDL
                     PSIVEELDLEDVITIGKDFLDNADTSDFVIFPKSS"
     gene            2812..3354
                     /gene="pgsA"
                     /locus_tag="VBE34_001309"
     CDS             2812..3354
                     /gene="pgsA"
                     /locus_tag="VBE34_001309"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEO7264026.1"
                     /translation="MVKKENIPNLLTVVRILMIPLFIVLTSVTTSTTWHIVAAIVFAI
                     ASLTDYLDGYLARKWQVVTNFGKFADPLADKMLVMSAFIMLVGLDLAPAWVSAIIICR
                     ELAVTGLRLLLVETGGTVLAAAMPGKIKTATQMFAVIFLLVHWMTLGNIMLYIALFFT
                     LYSGYDYFKGAGFLFKDTFK"
     gene            3354..4193
                     /locus_tag="VBE34_001310"
     CDS             3354..4193
                     /locus_tag="VBE34_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO7264027.1"
                     /translation="MTNIITVNNLFFKYDSNQTHYQLENVSFHVKQGEWLSIIGHNGS
                     GKSTTVRLIDGLLEAESGQIIIDGQELTEDNVWELRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIPLKDMKERVDQALDLVGMSEFKMREPARLSGGQKQRVAIAGAVAMRPQ
                     VIILDEATSMLDPEGRLELIRTIRAIRQKYNLTVISITHDLDEVALSDRVIVMKNGKV
                     ESTSTPKALFGRGNRLISLGLDVPFTSRLMAELAANGLDIGTEYLTEKELEEQLWELN
                     LKM"
     gene            4169..5011
                     /locus_tag="VBE34_001311"
     CDS             4169..5011
                     /locus_tag="VBE34_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO7264028.1"
                     /translation="MGIEFKNVSYTYQAGTPFEGRALFDVNLKIEDVSYTAFIGHTGS
                     GKSTIMQLLNGLHIPTKGEVIVDDFSIKAGDKNKEIKFIRQKVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGISQIEAERLAEEKLRLVGISEDLFDKNPFELSGGQMRRVAIAGIL
                     AMEPKVLVLDEPTAGLDPKGRKELMTLFKNLHKKGMTIVLVTHLMDDVADYADYVYVL
                     EAGKVTLSGQPKQIFQEVELLESKQLGVPKITKFAQRLSHKGLNLPSLPITINEFVEA
                     IKHG"
     gene            5004..5798
                     /locus_tag="VBE34_001312"
     CDS             5004..5798
                     /locus_tag="VBE34_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO7264029.1"
                     /translation="MDKLILGRYIPGNSLIHKLDPRSKLLAMLLFIIIVFWANNVVTN
                     VIVFIFTLVIVGLSQIKFSYFFNGIKPMVGIILFTTLFQMLFAQGGQVIFSFWIFSIT
                     SLGLQQAALIFMRFVLIIFFSTLLTLTTTPLSLADAVESLLKPLEVLRVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIHKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGANRSKYRLLKWTVRDTFSILLMLLLGLSLFLLKN"
     gene            6014..6553
                     /locus_tag="VBE34_001313"
                     /pseudo
     CDS             6014..6553
                     /locus_tag="VBE34_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            6659..7363
                     /locus_tag="VBE34_001314"
     CDS             6659..7363
                     /locus_tag="VBE34_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEO7264030.1"
                     /translation="MNKRRKLSKLNVKKQHLAYGAITLVALFSCILAVTVIFKSSQVT
                     TESLSKADKVRVAKKSKMTKATSKSKVEDVKQAPKPSQASNEAPKSSSQSTEANSQQQ
                     VTASEEAAVEQAVVTENTPATSQAQQTYAVTETTYKPAQHQTSGQVLSNGNTAGAVGS
                     AAAAQMAAATGVPQSTWEHIIARESNGNPNVANASGASGLFQTMPGWGSTATVQDQVN
                     SAIKAYRAQGLSAWGY"
     gene            complement(7498..8370)
                     /gene="sdaAA"
                     /locus_tag="VBE34_001315"
     CDS             complement(7498..8370)
                     /gene="sdaAA"
                     /locus_tag="VBE34_001315"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEO7264031.1"
                     /translation="MFYTIEELVEQANSQHKGNIAELMIQTEIEMTGRSREEIRYIMS
                     RNLEVMKASVIDGLTPSKSISGLTGGDAVKMDQYLQSGKTISDTTILAAVRNAMAVNE
                     LNAKMGLVCATPTAGSAGCLPAVISTAIEKLNLTEEEQLNFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSASAMAAAALVMAAGGTPFQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNALGSSFALVAADMALAGIESQIPVDEVIDAMYQVGSSLPTAFRETAEGGLAA
                     TPTGRRYSKEIFGE"
     gene            complement(8385..9053)
                     /gene="sdaAB"
                     /locus_tag="VBE34_001316"
     CDS             complement(8385..9053)
                     /gene="sdaAB"
                     /locus_tag="VBE34_001316"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEO7264032.1"
                     /translation="MKHLKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGEPSE
                     VTFHLYNSFAKTYQGHGTDKALVAGILGMDTDNPDIKNSLEIAHQKGIKIYWDILKDS
                     NSPHPNTAKITVKNGDRSMSITGVSIGGGNIQVTELNGFSVSLTMNTPTLIIVHQDIP
                     GMIAKVTDILSDFNINIAQMNVTRESAGEKAIMIIEVDSRDCQQAVKKIEAIPHLHNV
                     NFFD"
     gene            9299..10420
                     /gene="mnmA"
                     /locus_tag="VBE34_001317"
     CDS             9299..10420
                     /gene="mnmA"
                     /locus_tag="VBE34_001317"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEO7264033.1"
                     /translation="MTDNSNIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVTRDENGIVHMLRGADNNKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKPEVRRIAEEAGLATAKKKDSTGICFIGEKNFKDFLGQYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNKPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLTASEIHFTRDMPNEFKLECTAKFRYRQPDSKVTVYVKGNQARVVFD
                     DLQRAITPGQAVVFYNEQECLGGGMIDQAYRDDKICQYI"
     gene            10452..11063
                     /locus_tag="VBE34_001318"
     CDS             10452..11063
                     /locus_tag="VBE34_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEO7264034.1"
                     /translation="MISKFILAFMAFFAIMNPISNLPAFMALVADDDQKISRRIAAKG
                     VLLAFVIIVIFVLSGHLLFNLFGITLAALKISGGILVGIIGYKMINGIHSPTNKNLEE
                     HKDDPMNVAVSPLAMPLLAGPGTIATAMGLSSGGLSGKLITILAFAILCVIMYVILIS
                     ANEITKFLGKNAMTIITKMMGLILMTIGIEMLITGIKIGFHLT"
     gene            11233..13134
                     /gene="mnmG"
                     /locus_tag="VBE34_001319"
     CDS             11233..13134
                     /gene="mnmG"
                     /locus_tag="VBE34_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEO7264035.1"
                     /translation="MTHNFAENYDIIVVGAGHAGVEASLAASRMGCKTLLATINLEML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KALYAQTMKQTVEKQENLTLRQAMIDEILVEDGKVVGVRTATNQKFSAKSVVITTGTA
                     LRGEIILGDLKYSSGPNNSLASVTLADNLRDLGLEIGRFKTGTPPRVKASSINYEKTE
                     IQPGDEQPNHFSFMSRDEDYITDQVPCWLTYTNTLSHDIINQNLHRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRYTEEVYVQGLSTSLPEDVQVDLLRSIKGL
                     ENAEMMRTGYAIEYDIVLPHQLRATLETKVIAGLFTAGQTNGTSGYEEAAGQGLVAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGYEIGLVDEERYAIFKKRQMQFENELERLDSIKLKPVSETNKRIQELGFKPLTD
                     ALTAKEFMRRPQITYAVATDFVGCADEPLDSKVIELLETEIKYEGYIKKALDQVAKMK
                     RMEEKRIPPHIDWDDIDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEG
                     RQKGRKNIN"
     gene            13201..15183
                     /locus_tag="VBE34_001320"
     CDS             13201..15183
                     /locus_tag="VBE34_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEO7264036.1"
                     /translation="MKRFRFATVHLVLIGLILFGLLAICVRLFQSYTALLLAIFVALS
                     FVVALLYYQKITYELSEVEQIELLNDQTEVSLKSLLEQMPVGVIQFDLETNDIEWFNP
                     YAELIFTGDNGHFQSATVKDIITSRRNGTAGQSFEYGDNKYSAYLDTETGVFYFFDNF
                     MGNRRNYDSSMLRPVIGIISIDNYDDIMDTMLEADMSKINAFVTSFISDFTQSKNIFY
                     RRVNMDRYYIFTDYSVLNTLIKDKFDILNEFRKRAQENHLSLTLSMGISYGDGNHNQI
                     GQIALENLNTALVRGGDQIVVRENDSSKKALYFGGGAVSTIKRSRTRTRAMMTAISDR
                     LKVVDSVFIVGHRKLDMDALGASVGMQFFASNIVNASYVVYDPNDMNSDVERAIDYLQ
                     EDGETRLVSVERAFELITQNSLLVMVDHSKTALTLSKEFFNKFADVIVVDHHRRDEDF
                     PKNAVLSFIESGASSASELVTELIQFQQAKDKLSRSQASILMAGIMLDTRNFASNVTS
                     RTFDVASYLRGLGSNSMAIQKISATDFDEYRLINELILKGERIYDNIIVATGEEHKVY
                     SHVIASKAADTMLTMAGIEATFVITKNSSNIGISARSRNNINVQRIMEKLGGGGHFSF
                     AACQIQDKSVKQVRRMLLEIIDEDLRENSTVENRRD"
     gene            15189..15641
                     /gene="rplI"
                     /locus_tag="VBE34_001321"
     CDS             15189..15641
                     /gene="rplI"
                     /locus_tag="VBE34_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEO7264037.1"
                     /translation="MKVIFLQDVKGKGKKGEVKEVPTGYAQNFLLKKNLAKEATTQAI
                     GELKGKQKSEEKAQAEILAQAKELKTQLESETTRVQFIEKVGPDGRTFGSITAKKIAE
                     ELQKQYGIKIDKRHIDLDHTIRAIGKVEVPVKLHKQVSSQIKLDIKEA"
     gene            15684..17039
                     /gene="dnaB"
                     /locus_tag="VBE34_001322"
     CDS             15684..17039
                     /gene="dnaB"
                     /locus_tag="VBE34_001322"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO7264038.1"
                     /translation="MVEVSELRVQPQDLLAEQAVLGSIFISPEKLIMVREFISPDDFY
                     KYSHKVIFRAMITLADRNDAIDAATVRNILDDQGDLQNIGGLGYIVELVNSVPTSANA
                     EFYAKIVSEKAMLRDIISKLTDTVNMAYEGNDSDEIIATAEKALVDINEHSNRSGFRK
                     ISDVLKVNYENLELRSQQTSDVTGLPTGFRDLDRITTGLHPDQLIILAARPAVGKTAF
                     VLNIAQNVGTKQNRPVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNVT
                     IAQGALADAPIYIDDTPGIKITEIRARSRKLSQEVDDGLGLIVIDYLQLISGTRPENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYRREGEEAEEIVEDNTVEVILEKNRAGARGTVKLMFQKEYNKFSSIAQFE
                     E"
     gene            17051..>17267
                     /locus_tag="VBE34_001323"
     CDS             17051..>17267
                     /locus_tag="VBE34_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264039.1"
                     /translation="MSDAFADVAKMKKIKEDIKSHEGQMVELTLENGRKREKNKIGRL
                     IEVYPSLFIVEYKDTAAVPGAIDNTYVE"
BASE COUNT         5696 a         2698 c         3369 g         5504 t
ORIGIN      
        1 caaggtataa aaaatgggag taaatattgg ttgacgactt tgtcaatttt gaagaagatt
       61 gaaatttaga attaatcata tcttggatgt ttaatctttt ttaacgcctt taaaaatgat
      121 ataatcttag ctaacaatac agaacacaaa ggatattgaa tgaaaatagt tgaaggcgtt
      181 tctctacatt taattaaaaa ccaacaattt aagactaatc atcttacttt tcgattttca
      241 ggtgatttta ataataaaac agtagcacgt agatcgctcg ttgctcaaat gttagttact
      301 gcaaatgcta agtaccctaa ggtacaagaa tttcgagaaa agttagcttc cttatatggt
      361 gctagtttat caactaagat ttctactaag ggtcttgttc acattgttga tattgatatt
      421 gtatttgtta aaaatacctt tactttagaa caagaaaata tcgttgaaca aattattaca
      481 ttcttggagg atatgctgtt ttctccttta atttctttag aacagtatca aacatctatt
      541 tttgatactg agaagaaaaa ccttattcaa tatttagagg ccgatattga agataatttt
      601 tatagcagtg acttagcact aaaatcttta ttctacaata ataagacact tcgtttacct
      661 aagtatggta cagcatcttt agtggaatca gaaaattcat ttacagctta tcaagaattt
      721 cagaagatgc ttaaggaaga tcaattagat atttttgtcg taggagattt tgatgactat
      781 cgaatgattc aggcatttaa tcgtatggca tttgagccac gtcacaaagt attagctttt
      841 gattatacgc agacttatga aaatatcacg agaagtcaag tagaagataa ggacgttaat
      901 caatctatta tgcaattggc ttatcatctt ccaataactt ataaagatga agattatttt
      961 gctttaatag tatttaacgg tttatttggt gcttttgccc attccttgtt gtttacagaa
     1021 attcgagaaa agcaaggttt agcctatact ataggtagtc aatttgacag ctttacaggt
     1081 ctctttacga tatatgcagg tattgataaa gagaatcgtg agcgtttttt gaagcttatt
     1141 aataagcaat tcaataatat taagatgggc aggttctctt ctacattatt gaaacaaaca
     1201 aaagatatat tgaaaatgaa ctatgtttta gcttctgata atcctaaggt catagttgac
     1261 cacatttatc atgaacatta tttagatcag tttcatactt cagcactatt cattgacaaa
     1321 gtggacgatg taacaaaatc agatattgtc agcgtagcaa caaaattaaa attacaagct
     1381 ttttattttt tggaaggaaa ctaggatgac agttaataaa ataacttatc agaaccttca
     1441 ggaggaagta tataagttaa cattagagag cgggctaaat gtatatctta ttccaaaacc
     1501 atcatttaag gaaactgttg gagtattgac agcaaatttt ggatcactcc atacaagata
     1561 tacgaggaat ggttgtgtag aacattatcc ggcaggaatt gctcacttct tagaacataa
     1621 attatttgaa ctggataaag gacaagacgc tgcaactcaa tttactaaat atggcgccga
     1681 gagtaatgcc tttacaactt ttgataaaac tagtttttat ttttcaacta taagtcacat
     1741 tacaaactgc ttagatatac tacttgactt tgtattgaca acaaacttta cagaagagtc
     1801 aattactaaa gaaaaagaca ttatcaaaca agaaattgaa atgtatcaag atgacccaga
     1861 atataggctt tatcaaggag tgttatcgaa cctttacccc aactctccat tagcatttga
     1921 tatcgctgga gattaccaat caatctctca aataacctta accgatttac aagaaaatca
     1981 caaagacttt tatcagttgt ctaatatgaa tttagttctt gttggacaat ttagtccaca
     2041 agaaattata acttaccttc aaaaaaattc tcatttaaca aattactcac agaatattga
     2101 ccgagattct attagtcttg aacctgttat aaaaaacaac tcttgccata tgacagtgac
     2161 aaaacctaaa ttagctattg gttatcgaaa atcaagtcat atgagacatg gttcttacct
     2221 aaaagagaaa atcggattac agctattttt tgcaatgctt ttaggatgga cttctacaat
     2281 taatcaagac tggtacgaat ctggtcaaat tgatgactct tttgatatag aaattgaagt
     2341 tcatccagat tttgagtgcg ttattatttc tttagatacg acagagccaa ttgctttttc
     2401 aactcagttg aggttattgc taaaaaatgc tttgcaatca tctgatctta ccgaaagcca
     2461 cttacaaaac gttaagagag agctctatgg cgatttttta aggagcttag attctattga
     2521 aaatctcgca atgcaattcg tcacctattt atatgatgga aaaacgatgt atttagattt
     2581 gccatctatc gttgaagaat tagacttgga agatgtcatt acgattggta aggatttctt
     2641 agataatgct gacacatctg acttcgttat ttttccaaaa tcttcgtaaa taatctgaaa
     2701 ttttgatata ataaggaagc ttagacatct ataaagttgg tatgtcaaaa gtcttttact
     2761 agttttgaaa taacataata taggaatact atgtttccaa aaggagaatt tatggtgaag
     2821 aaagaaaata ttccgaatct attaactgta gtcagaattt taatgattcc gttatttata
     2881 gtattaactt cagtaacaac aagtacaact tggcatattg ttgcagctat tgtatttgct
     2941 attgcaagct tgaccgatta cctagacggt tatctagcac gtaaatggca agtagtaaca
     3001 aattttggga aatttgcaga tccattagct gataaaatgc tagttatgag tgcttttatc
     3061 atgttagttg gactagactt agcaccggct tgggtttcag ctattatcat ttgtagagag
     3121 ctagcagtta caggtttgcg tttactttta gttgaaacag gtggaacagt acttgctgca
     3181 gctatgccag ggaaaataaa gacggctact caaatgtttg cagttatttt tttgttagta
     3241 cattggatga cacttggtaa catcatgtta tacattgctt tattctttac cttgtattca
     3301 ggctacgact attttaaagg tgcaggcttc ttatttaagg atacttttaa ataatgacaa
     3361 atattattac tgttaataat ctctttttta aatatgatag taatcaaaca cattatcaat
     3421 tggagaatgt atcgtttcat gtgaaacaag gagaatggct atctattatc ggtcataatg
     3481 gttctgggaa atccactact gttcgtttaa tagatgggct tttagaggcg gagtcaggcc
     3541 agattatcat tgatggacaa gagttaaccg aagataatgt ctgggagtta cgtcacaaga
     3601 ttggcatggt atttcaaaat cctgataacc aatttgtagg ggcaactgtt gaagatgacg
     3661 tcgcctttgg tttggaaaat aaaggtattc ctttgaaaga catgaaagaa agagtggacc
     3721 aagcacttga tttggtaggc atgtctgaat ttaaaatgag ggagcctgca cgtttatctg
     3781 ggggacaaaa gcagcgtgta gccattgcag gagctgtagc tatgcgtcca caagttatca
     3841 tcttggatga ggcgactagt atgcttgatc ctgagggacg attggaactg ataagaacga
     3901 tacgagctat tcgtcaaaaa tataatctta ctgttatttc aattacacac gacttagatg
     3961 aagttgcatt gagtgatcgt gtcatcgtta tgaaaaatgg taaagttgag tcgacatcca
     4021 ctccaaaagc gttatttggt cgtggaaatc gtttgattag tttaggttta gatgttccct
     4081 ttacaagtag gttaatggca gaactggctg ctaatggtct tgatataggg acagagtatc
     4141 ttacagagaa ggaattagaa gaacaattat gggaattgaa tttaaaaatg taagttatac
     4201 ctatcaagcc ggcactcctt ttgaagggcg tgcccttttt gacgtcaatc tgaaaattga
     4261 agatgtttcc tataccgcgt tcattgggca cacaggttct ggaaaatcaa ctattatgca
     4321 acttttgaat ggtttacata ttcctacaaa aggtgaggta attgtcgatg atttttctat
     4381 taaagcaggg gacaagaaca aagaaatcaa atttataagg caaaaagttg gtttagtttt
     4441 tcaatttcca gaaagtcagc tttttgaaga gacagtttta aaggatgttg cttttggacc
     4501 acaaaatttt ggtatttctc agattgaagc tgaaaggctg gctgaagaaa aattaaggtt
     4561 agttggtatc agtgaggatt tattcgataa aaatccattt gaactttctg gagggcagat
     4621 gaggcgggtt gctatagctg gtattttagc gatggaaccc aaagtactag tactggatga
     4681 gccaacagct ggacttgatc ctaagggaag aaaagaatta atgactcttt ttaaaaatct
     4741 tcataaaaaa ggaatgacta tcgtcttagt gactcactta atggacgatg tagcggatta
     4801 tgctgactat gtgtatgttt tagaagcagg gaaagtaacc ttatcaggac aaccaaaaca
     4861 gatttttcaa gaagtagaac ttttagaaag taaacaatta ggagttccca aaatcaccaa
     4921 gtttgctcaa agactatctc ataagggatt aaatttacct agtttaccaa ttactattaa
     4981 cgaatttgtg gaggctatta agcatggata aattgatttt gggacgttac ataccaggta
     5041 attctctcat tcataaatta gatcctcgaa gtaagttact agccatgttg ctttttatca
     5101 ttattgtttt ttgggcaaat aatgtagtga caaatgtgat cgtattcatt tttacgttgg
     5161 ttattgtggg tttatctcag ataaaatttt cctatttttt caatggtatt aaaccgatgg
     5221 ttggtattat tttatttaca accctgttcc agatgctgtt tgcacaaggc gggcaggtta
     5281 tcttctcgtt ttggattttt agcatcacta gccttgggtt acaacaagca gcacttattt
     5341 ttatgagatt tgttttaatt atcttctttt cgacattact taccttaact actactccgt
     5401 taagtttagc agatgctgtt gaatctttgt taaaaccatt ggaagtactg agagtaccag
     5461 cacacgagat tggtttgatg ttatccctta gcttacgttt cgttcctact ttaatggatg
     5521 atacaacacg tatcatgaat gctcaaagag ctcgtggagt tgattttgga gagggaaacc
     5581 taattcataa ggttaagtcc attatcccta ttttgattcc attatttgct tctagtttta
     5641 aaagagcaga tgcgttggca attgctatgg aagcaagagg ttatcaaggt ggagccaatc
     5701 gtagtaagta ccgtttattg aagtggactg ttcgggatac ttttagtata ttactgatgt
     5761 tattattggg tttgagtttg tttctattaa aaaattaaaa ggttaaggtc agtttatggt
     5821 cttagccttt tcatttatat aatgtgaaaa gctcaacaac tttacttaaa gaaaactaaa
     5881 aattgttttt tggaggcctt ttctttatta atttacaatt tatttgtaat aaatttcatc
     5941 taaggcaaaa caattagtaa tatttacaag gtatgatagt acgcgtatca attaatgaaa
     6001 gagagcatat attatgtcaa taacctcggt taaaaaatca aaaccattta aattaggagt
     6061 ggcaggtctt ttagtgggtg cttcattagc tttaccactt tcagtaagcg cagcatctta
     6121 taccgtgaaa tcaggtgata ccttatcagc tattgctaaa aatcataaaa ctacggtaca
     6181 atagttagtg tctctcaata gtatcagtaa cgctgatgtc atcagtatag gtgatgtttt
     6241 aaaattggat aattctacag ctagtcaagc agaagcaaaa tctcaaccaa caattgaaaa
     6301 ttcaatgaat tcttcatcaa atttgagttc aagtgattca gctgcaaaag aagaaatagc
     6361 tcgtcgtgaa tcaaatggta gttatactgc acagaatgga caatattatg gaagatatca
     6421 actgtctcaa tcttacctaa atggcgactt atctcctgaa aatcaagaaa aagtagcgga
     6481 caattatgtg gcttctcgtt acggatcttg gtcggcagcg ctatcatttt ggaatagtaa
     6541 cggttggtat taataaaagt tgataaaaca aagagattta cttaaaatta aatctctttg
     6601 taatattttt aacatctatt caattgcaag gtaacaaata aagtataata taggtaacat
     6661 gaataaaaga agaaaattat caaaattgaa tgtaaaaaaa caacatttag cttatggagc
     6721 tatcacttta gtagcccttt tttcatgtat tttggctgta acggtcatct ttaaaagttc
     6781 acaagttact actgaatctt tgtcaaaagc agataaagtt cgcgtagcca aaaaatcaaa
     6841 aatgactaag gcgacatcta aatcaaaagt agaagatgta aaacaggctc caaaaccttc
     6901 tcaggcatct aatgaagccc caaaatcaag ttctcaatct acagaagcta attctcagca
     6961 acaagttact gcgagtgaag aggcggctgt agaacaagca gttgtaacag aaaatacccc
     7021 tgctaccagt caggcacaac aaacttatgc tgttactgag acaacttaca aacctgctca
     7081 acaccagaca agtggccaag tattgagcaa tggaaatact gcaggggcgg tcggatctgc
     7141 tgctgcagca caaatggctg ctgcaacagg agtccctcag tctacttggg aacatattat
     7201 tgcccgtgaa tcaaatggta atcctaatgt tgctaatgcc tcaggagctt caggactttt
     7261 ccaaacgatg ccaggttggg gttcaacagc tacagttcag gatcaagtta attcagctat
     7321 taaagcttat cgtgctcaag gtttatcagc ttggggttac tagtgaatag ctataaatta
     7381 actttacact attgtcaata ataattacag attgctaaaa caaataaata aaaacgctac
     7441 agtaaagctg tagcgttttt atttattttc agggaaagag tagaaaattg ataaacttta
     7501 ttccccaaaa atttctttgc tataacgtct tcctgtcggc gtggcagcaa gtcctccttc
     7561 tgcagtttca cgaaaagcag tcggtaaact tgatccaact tgatacattg catcaataac
     7621 ttcatctact ggaatttgcg attcaatacc agccaaggcc atatcagcag caacaagtgc
     7681 aaaacttgat ccaagagcat tccgcttcac acaagggact tcaactaaac ctgcaacagg
     7741 gtcacagata agtccaagca tatttttaat aacaaatgct atagcttggc tagcttggaa
     7801 aggagtacct ccagcagcca taactaaagc agccgcagcc atagcactag ctgacccaac
     7861 ttcagcttgg caacctcctt ctgcacctga gatagaggca ttattaccaa tgacaagacc
     7921 aaatgcgccg gctgtaaata gaaaattaag ttgctcttct tctgttaaat taagcttttc
     7981 aatggctgta gaaatcacag ctggtaaaca tcctgcacta cctgcagttg gtgttgcaca
     8041 gaccagtccc atcttagcat ttaactcatt aacagccata gcattcctaa cggcagctag
     8101 gattgtggta tctgaaatag tttttcctga ttgtaaatat tgatccatct tgacagcatc
     8161 accgcctgtt aaaccactga ttgatttact aggggtcaat ccatcaataa cagaagcttt
     8221 cataacctca aggtttcggg acataatata acgaatttct tcacgacttc taccagtcat
     8281 ttcaatttcc gtttggatca tgagctctgc tatgttaccc ttatgttggc tattagcttg
     8341 ctctaccagc tcttcaattg tataaaacat agttaccctt tctcttaatc aaagaagttg
     8401 acattgtgta aatgaggaat agcttctatc tttttgacag cttgttgaca atctcgtgaa
     8461 tctacttcta taatcatgat agctttttct cctgcacttt cgcgtgttac attcatttga
     8521 gcaatattaa tgttaaagtc agataaaata tcagttactt tagcaatcat gccaggtata
     8581 tcttgatgaa cgataattaa agtaggagta ttcattgtta atgaaactga gaatccattc
     8641 aactcagtaa cttggatgtt tccgccacca attgacactc ctgtaatact catgctccta
     8701 tccccatttt taacagttat tttcgcagta tttggatgtg gtgaattact atcttttagg
     8761 atatcccagt aaatcttaat tcctttttga tgagcaattt caagagagtt tttaatatct
     8821 ggattatctg tatccattcc tagaatccct gcaaccaatg ctttatcagt accgtgtcct
     8881 tggtaagttt tagcaaaaga attgtataaa tgaaaggtta cttcactagg ttcaccaaaa
     8941 atagaatgga caactttacc aatgcggaca gctcctgcag tatgactact tgatggtcca
     9001 atcataacag gaccaataat gtcgaagacc gattgaaatt ttaaatgttt catagttttt
     9061 cctcgtgatt tttgtacatt ctcattataa cagattattt ctaaaaaata tcataaaaag
     9121 ataaaaaaac tgcaatgtaa taaatattta tcacaacaaa aacatacaac ttgacaacaa
     9181 atagtgctat aatatataag cacttttata gtgtttctta gtcaatatga ttgatctaag
     9241 aacaacttgg cccttcaagc ctaagttaaa aataaaactt tttgatggga gaattcttat
     9301 gactgataat tcaaacattc gtgtcgttgt aggcatgagt ggaggtgttg attcatcagt
     9361 aactgccctg cttttaaaag agcaaggtta tgatgttatt ggtgttttta tgaaaaattg
     9421 ggatgacaca gatgaatttg gtgtttgtac ggctactgaa gattataaag atgttgctgc
     9481 tgttgctgat cagatcggca taccatacta ctctgtcaac tttgaaaaag agtattggga
     9541 cagggttttc gaatattttt tagcagaata tcgagcaggt cgtacgccaa atccagatgt
     9601 tatgtgtaat aaggaaatta aatttaaagc atttctagat tatgcgatga cattaggtgc
     9661 tgactatgta gcaacaggtc attatgcaca agtaacacgt gatgagaacg gaatagttca
     9721 tatgcttaga ggagcagata ataataagga tcaaacttat tttctaagtc aattatctca
     9781 ggagcaattg caaaaaacgt tatttccact tggtcattta caaaaaccag aagttcgccg
     9841 tatagctgaa gaagcaggac ttgcgaccgc taagaaaaaa gactctactg gtatttgttt
     9901 tattggagaa aaaaatttca aggatttcct gggtcaatat ttaccggctc aaccaggtcg
     9961 tatgatgact gttgatggtc gtgacatggg tgaacatgct ggtttaatgt attatactat
    10021 tggtcaaaga gggggccttg gtattggtgg gcaacatggt ggagataata aaccttggtt
    10081 tgtagttgga aaggatttaa gtaaaaacat tctttatgta ggtcaaggtt tctatcatga
    10141 ctcattgatg tcaactagct tgacagcttc tgaaatccac tttacacgtg atatgcctaa
    10201 tgaatttaag ctagaatgta cagctaagtt cagatatcgt caacctgaca gtaaagttac
    10261 agtttacgta aaaggtaatc aagcaagggt tgtgtttgat gatttacaac gagcaatcac
    10321 accaggtcaa gctgttgttt tttataatga acaagaatgt ttaggcggtg ggatgattga
    10381 tcaggcttat cgtgatgata aaatctgtca atacatttaa ttgacactta attattaagt
    10441 gaggtaatcg tatgatttca aaatttattt tagctttcat ggcttttttc gccattatga
    10501 atcccatttc taatttgcca gcttttatgg ctttagtagc tgatgatgat cagaagattt
    10561 cacgtcggat tgcagcaaaa ggagtattac ttgcttttgt cattattgta atctttgtat
    10621 taagtggcca cttacttttt aacttatttg ggattaccct agccgcccta aaaatatctg
    10681 gtggaatatt ggtaggcatt ataggttata aaatgattaa tggtatacat tcaccaacca
    10741 ataaaaacct agaggagcat aaggatgatc caatgaatgt ggcagtttca ccattagcaa
    10801 tgcccctttt ggcaggtccg ggtactattg caactgctat ggggttatca agtggtggtt
    10861 tatctggcaa actcattact atacttgctt ttgccatatt atgtgtgata atgtatgtta
    10921 ttttaataag tgcaaacgaa attactaaat ttcttggaaa aaatgcaatg actataatca
    10981 ccaaaatgat gggacttatt ttgatgacta tcgggataga gatgctaata acaggcataa
    11041 aaattggttt tcatttaaca tagcctatcc cctctggttg acttttaaaa gataaatgct
    11101 aaaataaatt taacaataac tatgatggtc aaagctcatg gaatttgtaa gtctttttga
    11161 cgtacaattt tcgagagttt tggctatttt tgtgttttaa tgaattagac tttgaaattg
    11221 gaaaggaata aaatgacaca taattttgca gaaaactatg atattatcgt tgttggagcg
    11281 ggtcatgcag gtgtggaagc tagtctagct gctagtcgta tgggatgtaa gacacttcta
    11341 gccactatca acttagaaat gttagcattt atgccttgta atccttctat aggtggctct
    11401 gctaaaggta tcgttgtcag agagattgac gctttaggtg gtgaaatggg taaaaacatt
    11461 gataaaacct atatacaaat gaaaatgctt aatacaggta aggggcctgc tgtccgtgcc
    11521 ttaagagcac aagctgataa agccctctat gcacaaacaa tgaagcaaac tgtagaaaag
    11581 caagaaaatc ttaccttaag acaagctatg attgacgaaa tacttgttga agatggaaag
    11641 gttgtggggg ttcgtacagc aactaatcag aaattttcag caaaatctgt tgttataaca
    11701 acaggtactg ctcttcgagg agagattata ttaggtgatc ttaagtactc ctcgggccct
    11761 aataatagtt tagcttctgt gacattggca gataatttaa gagacctagg attagaaatt
    11821 ggtcgtttca aaactggaac acctcctcgt gtaaaagcct cctctattaa ttatgaaaag
    11881 acagagatac aacctgggga tgagcaacca aatcattttt catttatgtc tagggatgaa
    11941 gattatatta ctgaccaggt accgtgctgg ttgacctata cgaatacttt gagtcatgac
    12001 atcatcaatc aaaatctaca ccgtgctcct atgttttctg gaatcgttaa gggagttggt
    12061 cctagatatt gtccgtcaat tgaagataaa attgtgcgtt ttgctgataa agagcgccac
    12121 caactcttct tagaaccaga aggtagatac actgaagaag tttatgttca agggctatca
    12181 acgagtcttc ctgaggatgt tcaagttgat ttattacgtt ctataaaagg gcttgaaaat
    12241 gctgaaatga tgcgtacagg ctatgctatt gagtatgata ttgtattgcc tcatcaatta
    12301 agagcaacac tcgaaacaaa agtaattgct ggtttgttta ctgcaggaca gactaatggt
    12361 acttcaggtt atgaagaagc tgctggtcaa gggttggttg ctgggatcaa cgctgctctt
    12421 aaagttcaag ggaagccaga acttatttta aaacgaagtg atgcttacat tggtgtcatg
    12481 attgatgatt tagttaccaa agggactcta gaaccatatc gtctgttgac aagtcgcgca
    12541 gaatatcgtc tgattcttcg tcatgataat gcagatatgc gtttaacaga aatcggttat
    12601 gagattggcc tagtagatga agagcgttat gcgatattta aaaagcgtca aatgcaattt
    12661 gaaaatgaat tagaacgttt agactctata aagttaaagc cagtttctga gacaaataaa
    12721 cggattcaag aattgggctt taaacctttg acagatgctt taactgccaa agaatttatg
    12781 cgccgcccac aaatcacata tgcagtagca acggattttg ttggttgtgc tgatgagcca
    12841 ttagacagta aggttattga acttttggaa actgaaatta aatacgaagg ctatattaaa
    12901 aaggctttag atcaagttgc aaaaatgaaa cgaatggaag aaaaacgtat tccccctcat
    12961 attgactggg acgacattga ttctatcgca acagaagcta gacaaaaatt caaaaaaatt
    13021 aacccagaaa cgctaggtca ggctagtcgt atttcaggag ttaatccagc cgatattagt
    13081 attttaatgg tttaccttga aggaagacaa aaaggtcgta aaaatatcaa ttgataatag
    13141 tgttcgcccc tgtttatcag gggcgttttg tgttataatt aggtcttaag aggttattta
    13201 atgaaaagat ttcgttttgc aaccgtccat ttagttttaa taggcctgat tttatttgga
    13261 ctattagcta tttgtgttag gttatttcaa tcctatacgg ctctattatt ggctattttt
    13321 gttgctttat cttttgttgt tgccttatta tattaccaaa agataactta tgaactatct
    13381 gaagttgaac aaattgaatt acttaatgat caaacagaag tttctcttaa gagtcttctt
    13441 gaacaaatgc cagttggtgt tatacaattt gatcttgaaa caaatgatat tgagtggttt
    13501 aacccttatg cagaattgat ttttactggg gataatggtc attttcagtc tgctactgtt
    13561 aaagatatca ttactagtag acgtaatgga acggctgggc aatcatttga atacggtgat
    13621 aacaaatatt cagcttattt ggatacagag acgggtgttt tctatttctt cgataatttc
    13681 atggggaata gaagaaacta tgatagttca atgctacgtc cagttattgg tattatttcc
    13741 attgataact acgatgatat tatggatact atgttagagg ctgatatgtc aaagattaac
    13801 gccttcgtga ctagttttat ttctgatttt acacagtcta aaaatatttt ttatcgtcgt
    13861 gtcaatatgg atcgatacta tatttttacc gactatagcg ttttaaatac attgataaaa
    13921 gataaatttg atattttaaa tgagtttcga aaacgcgctc aggaaaacca tttatcatta
    13981 acattaagta tgggaatttc ttatggtgat ggtaaccata atcaaattgg gcaaattgct
    14041 ttagaaaatc ttaatacagc actcgttcgc ggaggagatc aaatagtagt acgtgaaaat
    14101 gatagtagta aaaaagcact ttactttggt ggtggtgctg tttcaactat taaacgttca
    14161 cgaacacgta ccagagctat gatgactgct atttctgacc gtttgaaagt agttgatagt
    14221 gtctttattg tgggacacag gaaattggac atggatgcac taggtgcatc tgtagggatg
    14281 caattttttg ctagtaatat tgttaatgcc agttatgtag tttatgaccc aaatgacatg
    14341 aattctgatg ttgaaagagc aattgattac ttgcaagaag acggcgaaac acgtctcgta
    14401 agtgttgaga gagcttttga attaattact caaaattctt tattagtcat ggtagaccat
    14461 tctaaaacag ctttaacctt atctaaggaa ttctttaaca agtttgcaga tgttattgtg
    14521 gtagatcatc atagaagaga tgaagatttt ccaaaaaatg ctgttctttc atttatagaa
    14581 agtggcgcaa gtagtgccag cgaattagtt acagaattga ttcaatttca acaagctaaa
    14641 gataaattaa gccgcagtca agcaagtatt ttaatggctg gtatcatgct tgatacaaga
    14701 aattttgcat ctaatgtaac gagtagaact tttgatgtgg caagctattt acgcggttta
    14761 ggtagtaata gtatggctat tcaaaaaata tcagctacag attttgatga atatcgttta
    14821 attaatgaat tgattttaaa aggtgagcgt atttatgata acatcattgt tgctaccgga
    14881 gaagaacata aagtttactc gcatgttata gctagtaaag cagctgacac catgttgaca
    14941 atggcaggta ttgaggctac ttttgttatt actaaaaaca gttcaaatat tggtatttca
    15001 gcgcgcagta gaaataatat caatgtccag cgtattatgg aaaaacttgg tggtggcgga
    15061 cattttagtt ttgctgcttg tcaaatacaa gacaagtctg taaagcaagt tagacgtatg
    15121 ttactggaaa taattgacga agatttaaga gaaaatagta cagtcgaaaa tagaagggat
    15181 taattgagat gaaagttatt tttttacaag atgttaaggg gaaaggaaaa aaaggtgagg
    15241 ttaaggaagt acctactggt tatgctcaaa acttcttatt aaaaaagaat ctagctaaag
    15301 aagcaacgac acaagctatc ggtgaattaa aaggaaaaca aaagtccgaa gaaaaagcac
    15361 aagcagaaat tttagcacag gcaaaggagc tgaaaacaca attagaatcc gaaactaccc
    15421 gagtgcaatt tattgaaaag gtaggtccag atggtcgtac gtttggttct atcacagcca
    15481 aaaagattgc tgaagagtta caaaaacaat acggcattaa aattgacaaa cgtcatattg
    15541 atttagatca tactattaga gcgattggta aggttgaagt accagtaaaa ttacataaac
    15601 aggtttcttc acagattaag cttgatatca aagaagcgta aactgagaga tgataaaggt
    15661 tgacaagtca ggaggaatga agtttggtag aagtatctga gctaagagta caacctcaag
    15721 acctgttggc agaacaggcc gttctaggat ctatctttat ctcaccagag aaactgatta
    15781 tggtgagaga atttataagt ccagatgatt tttataaata ttcacacaaa gttatcttca
    15841 gagcgatgat taccttagct gatagaaatg atgcaattga tgctgctact gttagaaata
    15901 ttttagatga ccaaggtgat cttcaaaaca ttggtgggtt gggctatatt gttgaattag
    15961 ttaatagtgt tccaacaagt gctaatgcgg agttttatgc caaaatagtt tctgagaaag
    16021 ccatgttgcg agatatcatt tctaagttga cagatactgt caacatggcc tatgaaggaa
    16081 atgattctga tgaaattatt gccaccgctg agaaggcttt agttgatatt aacgaacaca
    16141 gtaatcgtag cgggtttaga aagatatctg atgttttaaa agttaattac gaaaacttag
    16201 aattacgttc acagcaaacc tcagatgtca caggtttacc aactggattt agagatttag
    16261 acaggattac tacaggttta catccagatc aattaatcat cttagcggct cgtcctgcag
    16321 taggtaaaac agcctttgtt ttaaatattg ctcaaaatgt tggaactaaa caaaatagac
    16381 ctgtagctat tttttcactt gaaatggggg ctgagagttt agttgaccgt atgttggcag
    16441 cagagggaat ggttgattct catagtttac gaactggtca gttgacggat caagattgga
    16501 ataatgtaac gattgctcaa ggagctttgg cggatgcgcc aatttatatt gatgatacgc
    16561 ccgggattaa aattacagaa attagagcac gctctaggaa gttatctcaa gaagttgatg
    16621 atggtttagg tttaatcgtc attgactacc tccaattaat ttcaggaact agaccagaaa
    16681 atcgtcagca agaagtttct gaaatttcaa ggcagttgaa gattttagct aaagaattaa
    16741 aggttcctgt tatcgcatta agccagctct ctcgtggggt tgagcaaagg caggataaac
    16801 gaccggtctt atctgatatt cgtgaatctg gatcgattga acaagatgct gacattgttg
    16861 ctttcttata tcgagatgat tattatcgta gagagggcga agaagcagag gaaattgtag
    16921 aagataatac tgttgaggtt attttagaaa agaaccgtgc tggtgcgcgt gggacagtaa
    16981 aattaatgtt tcaaaaggaa tataataaat tttctagtat agctcaattt gaagaatagg
    17041 agatgttaat atgagtgatg catttgcaga tgttgcaaaa atgaaaaaaa taaaagaaga
    17101 tatcaagtca catgagggac agatggtcga acttactctc gaaaatggtc gtaagaggga
    17161 aaaaaataaa attgggcgcc taattgaagt ttacccatct ttgtttatcg tagagtataa
    17221 ggatactgca gctgtaccag gagcaattga taatacttat gtagagt
//
LOCUS       DATRUK010000041        17213 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.041, whole genome shotgun sequence.
ACCESSION   DATRUK010000041 DATRUK010000000
VERSION     DATRUK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17213
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.041"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(37..603)
                     /locus_tag="VBE34_001324"
     CDS             complement(37..603)
                     /locus_tag="VBE34_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000596040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7264040.1"
                     /translation="MIKRPIHLSHDFLAEVIDKEAITLDATMGNGNDTVFLAKSSKKV
                     YAFDIQEEAIAKTKAKLTEQGISNAELILDGHENLEQYVHTPLRAAIFNLGYLPSADK
                     TVITKPHTTIKAIKNVLDILEVGGRLSLMVYYGHDGGKSERDAVIAFVEQLPQNNFAT
                     MLYQPLNQVNTPPFLIMVEKLQSYENEV"
     gene            complement(596..1531)
                     /locus_tag="VBE34_001325"
     CDS             complement(596..1531)
                     /locus_tag="VBE34_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747785.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEO7264041.1"
                     /translation="MKKRYRAINDYYRELFGEKIFKLPIDAGFDCPNRDGTVARGGCT
                     FCTVSGSGDAIVAPEAPIREQFYKEIDFMHRKWPEVNKYLVYFQNFTNTHAKLEIIKE
                     RYEQAINEPGVIGINIGTRPDCLPDETIYYLAELSERMHVTLELGLQTTYEATSALIN
                     RAHSYDLYKKTVKRVRELAPKVEIVSHLINGLPGETHDMMVENVRRCVTDNDIQGIKL
                     HLLHLMINTRMQRDYHEGRLRLLSQEDYISIICDQLEIIPKHIVIHRITGDAPRHMLI
                     GPMWSLNKWEVLNAIDKEMEKRQSYQGCKVEVKHD"
     gene            complement(1624..2265)
                     /locus_tag="VBE34_001326"
     CDS             complement(1624..2265)
                     /locus_tag="VBE34_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO7264042.1"
                     /translation="MLSRQNSKLIQAFIAIILFFSLGLVIKYWPDTVIAFDQTIQESV
                     RGQLPNLSTRFFKLITVVGNTVSQIAIAIMSVTFCYLKKWYPQARFIAVNAIISGICI
                     LSLKLIFQRVRPTLTHLVFAGGYSFPSGHSMGTFMIFGSSIILLQYYMPKSIWKLLCQ
                     GTLGLLIFLIGLSRIYLGVHFPTDVLAGFILAYGILNLTAYIFLSKDWLKKVK"
     gene            complement(2246..2830)
                     /locus_tag="VBE34_001327"
     CDS             complement(2246..2830)
                     /locus_tag="VBE34_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7264043.1"
                     /translation="MTNTRKMVTIAILSALSFVLMMVSFPLIPGAEFLKVDFSILPML
                     VAFILFDLKSSYGVLLLRSLLKVILANRGPETFIGLPMNMVALALFLASFAIFWKNRE
                     SAKDFIKASLFGTVSLTVSMVALNYVFAIPLYAIFANFDIRTFIGVGNYLLTMVIPFN
                     IVEGILISIVFYLTYVACLPILERYKKTNVISSK"
     regulatory      complement(2900..3045)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(3114..3713)
                     /locus_tag="VBE34_001328"
     CDS             complement(3114..3713)
                     /locus_tag="VBE34_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEO7264044.1"
                     /translation="MIRRYSANFLAILGIILVSSGIYWGWYNINQAHQADLTSQHIVK
                     VLDKSITHQVKGSENGELPVKKLDKTDYLGTLDIPNLKLHLPVAANYSFEQLSKTPTR
                     YYGSYLTNNMVICAHNFPYHFDALKNVDMGTDVYFTTTTGQIYHYKIGNREIIEPTAI
                     EKVYKTATSDNDWDLSLFTCTKAGVARVLVRCQLIDVKN"
     gene            complement(3706..4377)
                     /locus_tag="VBE34_001329"
     CDS             complement(3706..4377)
                     /locus_tag="VBE34_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264045.1"
                     /translation="MIYKKILKITLLLLFSLSTQLVSADTNDQMKTGSITIQNKYNNQ
                     GIAGGNLLVYQVAQAKDVDGNQVFTLTTPFQGIGIKDDDLTQVNLDSNQAKYVNLLTK
                     AVHKTQPLQTFDNLPAEGIVANNLPQGIYLFIQTKTAQGYELMSPFILSIPKDGKYDI
                     TAFEKMSPLNAKPKKEETITPTVTHQTKGKLPFTGQVWWPIPILIMSGLLCLIIALKW
                     RRRRD"
     gene            complement(4367..5242)
                     /locus_tag="VBE34_001330"
     CDS             complement(4367..5242)
                     /locus_tag="VBE34_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEO7264046.1"
                     /translation="MKKIRQHLVSILLVTIIAVGVGLMAYPSLANYWNSFHQSRAIMD
                     YQDRVTHMDENDYKKIINRAKEYNKQFKTSGMKWHMTSQERLDYNSQLAIDKTGNMGY
                     ISIPKINIKLPLYHGTSEKVLQTSIGHLEGSSLPIGGDSTHSILSGHRGLPSSRLFSD
                     LDKLKVGDHWTVSILNETYTYQVDQIRTVKPDDLRDLQIVKGKDYQTLVTCTPYGVNT
                     HRLLVRGHRVPNDNGNALVVAEAIQIEPIYIAPFIAIFLTLILLLISLEVTRRARQRK
                     KILKQAMRKEENNDL"
     gene            complement(5280..6788)
                     /locus_tag="VBE34_001331"
     CDS             complement(5280..6788)
                     /locus_tag="VBE34_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO7264047.1"
                     /translation="MKKKMIQSLLVASLAFGMAVSPVTPIAFAAETGTITVQDTQKGA
                     TYKAYKVFDAEIDNANVSDSNKDGASYLIPQGKEAEYKASTDFNSLFTTTTNGGRTYV
                     TKKDTASANEIATWAKSISANTTPVSTVTESNNDGTEVINVSQYGYYYVSSTVNNGAV
                     IMVTSVTPNATIHEKNTDATWGDGGGKTVDQKTYSVGDTVKYTITYKNAVNYHGTEKV
                     YQYVIKDTMPSASVVDLNEGSYEVTITDGSGNITTLTQGSEKATGKYNLLEENNNFTI
                     TIPWAATNTPTGNTQNGANDDFFYKGINTITVTYTGVLKSGAKPGSADLPENTNIATI
                     NPNTSNDDPGQKVTVRDGQITIKKIDGSTKASLQGAIFVLKNATGQFLNFNDTNNVEW
                     GTEANATEYTTGADGIITITGLKEGTYYLVEKKAPLGYNLLDNSQKVILGDGATDTTN
                     SDNLLVNPTVENNKGTELPSTGGIGTTIFYIIGAILVIGAGIVLVARRRLRS"
     gene            complement(6829..11133)
                     /locus_tag="VBE34_001332"
     CDS             complement(6829..11133)
                     /locus_tag="VBE34_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HEO7264048.1"
                     /translation="MLKKCQTFIIESLKKKKHPKEWKIIMWSLMILTTFLTTYFLILP
                     AITVEETKTDDVGITLENKNSSQVTSSTSSSQSSVEQSKPQTPASSVTETSSSEEAAY
                     REEPLMFRGADYTVTVTLTKEAKIPKNADLKVTELKDNSATFKDYKKKALTEVAKQDS
                     EIKNFKLYDITIESNGKEAEPQAPVKVEVNYDKPLEASDENLKVVHFKDDGQTEVLKS
                     KDTAETKNTSSDVAFKTDSFSIYAIVQEDNTEVPRLTYHFQNNDGTDYDFLTASGMQV
                     HHQIIKDGESLGEVGIPTIKAGEHFNGWYTYDPTTGKYGDPVKFGEPITVTETKEICV
                     RPFMSKVATVTLYDDSAGKSILERYQVPLDSSGNGTADLSSFKVSPPTSTLLFVGWSK
                     TQNGAPLSESEIQALPVSSDISLYPVFKESYGVEFNTGDLSTGVTYIAPRRVLTGQPA
                     STIKPNDPTRPGYTFAGWYTAASGGAAFDFNQVLTKDTTLYAHWSPAQTTYTINYWQQ
                     SATDNKNATDAQKTYEYAGQVTRSGLSLSNQTLTQQDINDKLPTGFKVNNTRTETSVM
                     IKDDGSSVVNVYYDRKLITIKFAKYGGYSLPEYYYSYNWSSDADTYTGLYGTTLAANG
                     YQWKTGAWGYLANVGNNQVGTYGMSYLGEFILPNDTVDSDVIKLFPKGNIVQTYRFFK
                     QGLDGTYSLADTGGGAGADEFTFTEKYLGFNVKYYQRLYPDNYLFDQYASQTSAGVKV
                     PISDEYYDRYGAYHKDYLNLVVWYERNSYKIKYLDPLDNTELPNFPVKDVLYEQNLSS
                     YAPDTTTVQPKPSRPGYVWDGKWYKDQAQTQVFDFNTTMPPHDVKVYAGWQKVTYRVN
                     IDPNGGRLSKTDDTYLDLHYGDRIPDYTDITRDYIQDPSGTYYYKYDSRDKDPDSTKD
                     AYYTTDTSLSNVDTTTKYKYVKDAYKLVGWYYVNPDGSIRPYNFSGAVTQDINLRAIW
                     RKAGDYHIIYSNDAVGTDGKPALDASGQQLQTSNEPTDPDSYDDGSHSALLRRPTMPD
                     GYRFRGWWYNGKIYNPYDSIDIDAHLADANKNITIKPVIIPVGDIKLEDTSIKYNGNG
                     GTRVENGNVVTQVETPRMELNSTTTIPENQYFTRTGYNLIGWHHDKDLADTGRVEFTA
                     GQSIGIDNNPDATNTLYAVWQPKEYTVRVSKTVVGLDEDKTKDFLFNPSETLQQENFP
                     LRDGQTKEFKVPYGTSISIDEQAYDEFKVSESITEKNLATGEADKTYDATGLQSLTVS
                     GDVDISFTNTRIKQKVRLQKVNVENDNNFLAGVVFDIYESDANGNKASHPMYSGLVTN
                     DKGLLLVDANNYLSLPVGKYYLTETKAPPGYLLPKNDISVLVISTGVTFEQNGNNATP
                     IKENLVDGSTVYTFKITNSKGTELPSTGGIGTHIYILVGLALALPSGLILYYRKKI"
     gene            complement(11153..11722)
                     /gene="lepB"
                     /locus_tag="VBE34_001333"
     CDS             complement(11153..11722)
                     /gene="lepB"
                     /locus_tag="VBE34_001333"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO7264049.1"
                     /translation="MKRQISSDKLSQELDRVTYQKRFWSVIKNTIYILMAVASIAILI
                     AVLWLPVLRIYGHSMNKTLSAGDVVFTVKGSNFKNGDVVAFYYNNKVLVKRVIAESGD
                     WVNIDSQGDVYVNQHKLKEPYVIHKALGNSNIKYPYQVPDKKIFVLGDNRKTSIDSRS
                     TSVGDVSEEQIVGKISFRIWPLGKISSIN"
     gene            complement(11706..11978)
                     /locus_tag="VBE34_001334"
     CDS             complement(11706..11978)
                     /locus_tag="VBE34_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264050.1"
                     /translation="MEDLKKLKRKDLLEIMLEQQLLIEKQEKEIQQLKEALDDKKIKI
                     SKAGSIAEASLMLSGIFETAQLAADTYLENVKNLVDKRGVDNEKTD"
     gene            complement(12260..13408)
                     /locus_tag="VBE34_001335"
     CDS             complement(12260..13408)
                     /locus_tag="VBE34_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO7264051.1"
                     /translation="MENKVKTVAVFSGYYLPFLGGIERYTDKMTADLVKRGYRVVIVT
                     TNHDDLPIIDEDKGRKIYRLPTKNIVKQRYPIINKNREYNTLMKHVSDENIDFVICNT
                     RFQLTTLEGLSFAKKHRLPSIVLDHGSSHFSVNNRFLDFFGAIYEHLLTARVKHYRPD
                     FYAVSKRSVEWLKHFNIEAKGVIYNSVSESLGSDFADTAYLEKSADDIFITYAGRIIK
                     EKGIELLLEAFSMSQYSENVYLQIAGDGPELAHLKEKYQSKQINFLGKLNFEQTMSLM
                     AQTDIFVYPSMYPEGLPTSILEAGLLSSAIIATDRGGTVEVIDSPELGIIMEENTQSL
                     HESLDLLVKDKALREKLQQNIAKRIKEHFTWEKTVEKLDYIIQKELNL"
     gene            complement(13398..14246)
                     /locus_tag="VBE34_001336"
     CDS             complement(13398..14246)
                     /locus_tag="VBE34_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7264052.1"
                     /translation="MKYLAGIVTFNPNIERLDQNIRAIYPQVSHIYIVDNGSKNKEEI
                     SQLVADYNEEGHLTVDYLTENKGIAYALNCIGQFAVAQEFDWFLTLDQDSVVLGDLID
                     NYENYLLLPKVGMLSCLYQDMNREDLVMQEFDYKEIEECITSAALMKTSVFEETSGFA
                     EEMFIDFVDSEMNYRLSEMGYKTYQVNFIGLLHEIGHSSRVKKFGHVFHVLNHSPFRK
                     YYMIRNAIYIIKKYGKKKRHKYLVFMRNEFVRVLVAEEQKSKKLVAMIKGLKDGLLMK
                     VDKYGK"
     gene            complement(14243..15667)
                     /locus_tag="VBE34_001337"
     CDS             complement(14243..15667)
                     /locus_tag="VBE34_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEO7264053.1"
                     /translation="MKLLKNMFYNTSYQLLTLLLPLVTVPYVSRVLSPQGIGINAYTS
                     SIVMYFTLFGALGISLYGNREIAFVQSNKYKRSKIFWELVVLKLASVSIATLLFFGFV
                     LLTNEWQLFYLIQGINLLATATDISWYFIGVEDFKIIVIRNTIVKLITVVLTFLVVKT
                     PDDLALYMFLIAFASLLGNLTVWHHLKHEIIKIPFSRLDILIHLRPTLMLFLPQITMQ
                     IYLSLNKSMLGAMDSVVSAGYFDQSDKIIRILFTIVSAIGGVFLPRLSSLFSSGKEKQ
                     AKALLLKLVDLSNAISMLMIAGVVGVSSTFAVFFFGKGYEAVGPLMAVESLMIICISY
                     GNALGTQYLLASRRTKAYTMSAVIGLVANVVLNILLIPILGAMGAIISTVITEFIVSL
                     YQAISLRDVFTFKELTRGMLRYLIAATLSGAVLYYINTQMSVSLVNYVIQSLVAVTIY
                     VGIVFITKAPVIQLFRDFRKEHRT"
     gene            complement(15667..17031)
                     /locus_tag="VBE34_001338"
     CDS             complement(15667..17031)
                     /locus_tag="VBE34_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEO7264054.1"
                     /translation="MNLKKVRLFEKFFLDKNLHKTYFILGLTITIVLSLLMPFFNEPD
                     GQYHLAVSGRISNNIIDTSYYGEYNIDSGMRRQSTSYRDGTRFEKYYLNKANFVDGNH
                     LPREINYSKHNFVFWGHLVPAVGLFLGKFVYPSMGVMITVARLFSSFVSITLVALIIR
                     YVKKGKLIYFAIFLSPVVLNSFASLSYDSTGYIVFGAFLSLLINLLVDKSLTKNRSIA
                     LIGLILLVIVACKQNYWLFLLLVIPVLLQVNNPKLMGYNQKLSHLFQGLKSKKLFWLC
                     LTFLSLAIVYVLTARHGGVLAVLRRYVMTFGYNYAGINILSNDITSWLASPYPSQNFI
                     PTWVSSVWYLMVFLVWFSEEKFVKSKNMGFLFLASFFVGILGVFFIMLEYRGAPTSYI
                     EGVQGRYFTVTLLLVQVFASSIPARLNEAGRKVVPYALTGLVIVSNALLLFDTVISLI
                     MRRN"
     gene            complement(17033..>17213)
                     /locus_tag="VBE34_001339"
     CDS             complement(17033..>17213)
                     /locus_tag="VBE34_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7264055.1"
                     /translation="SQLREAKAYLDFNQQRYKNAQNKDFVAILKQMMTKKRFYDSFSN
                     RIRDLVFLLKDIKRS"
BASE COUNT         5506 a         3266 c         2693 g         5748 t
ORIGIN      
        1 tagcaattat catttttgct aattgcctaa agatagttac acctcatttt catatgattg
       61 taacttttct accataatta aaaagggggg cgtattaact tgattgagtg gttgataaag
      121 cattgtagcg aaattattct gtggtaattg ttccacgaat gctatcaccg catctctctc
      181 actttttcca ccatcatgac cataataaac cataagtgat agacgtccac ctacttctaa
      241 aatgtctaaa acgtttttga ttgccttgat agttgtgtgt ggttttgtaa taactgtttt
      301 atccgctgag ggtaagtatc ccagattaaa tatagccgct cttaaaggtg tatgaacata
      361 ctgttccaaa ttttcatgcc catccaagat aagttcagcg ttagagatcc cttgctccgt
      421 gagctttgct ttcgtttttg ctattgcttc ttcttgaata tcaaatgcat aaactttttt
      481 agaacttttc gctaaaaaaa cagtatcatt cccatttccc atagttgcat caagcgttat
      541 tgcttcttta tcaataactt ctgctaaaaa atcatgagaa agatgaattg gtcttttaat
      601 catgttttac ctcaacttta catccttgat atgactgtct tttctccatt tctttgtcaa
      661 tggcattaag cacttcccat ttattaaggc tccacatcgg tccaattaac atatgacgtg
      721 gcgcatcacc agtaattcgg tgaataacaa tatgttttgg aataatctcc agttggtcac
      781 aaataatcga aatatagtct tcttgactta acaaacgtaa acgaccttca tggtaatctc
      841 tctgcattct tgtattaatc ataagatgca gaaggtgtaa ttttataccc tgaatatcat
      901 tatccgtaac acatcgacga acattttcca ccatcatatc atgtgtctcc cctgggagac
      961 catttattag gtgagaaacg atttctactt taggagctaa ttctcgtact ctcttaacag
     1021 tttttttgta aaggtcatat gaatgtgctc tatttattaa tgcagaagtt gcttcataag
     1081 ttgtctgaag gcctaattct aaagtcacat gcattctttc agaaagttca gcgagatagt
     1141 atatagtttc atcaggtaag caatccggcc ttgttccgat gttgatcccg ataactcctg
     1201 gctcattaat agcctgttcg taacgctctt taattatctc taacttagca tgggtattgg
     1261 taaaattttg aaaatagact aagtatttat taacctcagg ccactttcta tgcatgaaat
     1321 caatttcttt atagaattgt tcacgaatag gagcttctgg agcaactata gcatcccctg
     1381 aaccagaaac tgtgcaaaaa gtgcaccctc ctctagcaac tgttccatct ctgttaggac
     1441 agtcaaaacc agcatctata ggtaatttaa atattttttc tccaaagagt tctcgataat
     1501 aatcattaat cgcacgataa cgttttttca taggataatt gtatcacaat tttaactaaa
     1561 ataacctcac tactacaata aaactaaaaa agattggaac gtcagttagt cccaatcttt
     1621 tatttacttc actttcttta accaatcctt ggataaaaag atatacgcag ttagattcaa
     1681 aataccataa gcaagtataa aaccagctaa aacatctgtc ggaaaatgaa cccctaggta
     1741 aatacgagat aacccaatta aaaaaatgag caaacccaat gtaccttggc acaacagttt
     1801 ccatatactc ttaggcatat agtactgcaa taaaataata ctactcccaa atatcataaa
     1861 tgttcccatc gagtgcccac tgggaaacga atagccacct gcaaatacta aatgggttaa
     1921 agttggtctc actctttgaa aaataagttt taaagaaagt atacatatac cagagataat
     1981 agcatttact gcgataaatc tagcttgagg ataccacttc ttaaggtaac agaaagtgac
     2041 gctcataatc gcaatagcta tctggcttac agtattacca accacagtga ttaacttaaa
     2101 aaatcttgta gaaagatttg gcaactgtcc tctaacactt tcttgaatag tttggtcaaa
     2161 tgcgattaca gtgtcgggcc aatatttgat gaccaatcct aaactgaaaa ataagataat
     2221 agcaataaat gcttgaataa gtttactatt ttgacgagat aacattagtc tttttatatc
     2281 tttctaatat tggcaaacaa gccacgtaag ttagatagaa aacaatcgaa attaaaattc
     2341 cctcaacgat attaaatgga ataaccattg ttaaaaggta attgcctaca ccaataaatg
     2401 ttctgatatc aaagttagca aatatagcat acaaaggaat cgcaaagaca tagttgagag
     2461 ctaccataga tacagtcaag ctaactgtac caaataaact agctttaata aaatcttttg
     2521 cactctctct atttttccag aaaatagcga aacttgctaa aaatagagct agagcaacca
     2581 tattcatcgg taaaccgata aaggtttctg gaccacgatt agcaagtata acttttaaaa
     2641 gtgatcttaa taagagtaca ccataacttg atttcaaatc aaataaaata aaagcaacta
     2701 acatcggaag gattgaaaaa tcaaccttta aaaattctgc tcctggtatt aatggaaatg
     2761 aaaccatcat caatacaaaa gataaggcag aaagaatggc gattgtcacc attttacgtg
     2821 tatttgtcat aaaaaaattc ctccaattta aataaattga aagaagctcc aaaggtaagc
     2881 gtaggtacgc gaaaaaaacc tttgtcttct cccatccaga ctttactgtc ggttgtggaa
     2941 tctcaccaca tcagctttcg ctcgcggact gatgcttcac aactgacaaa taagttggaa
     3001 gcgattaccg ccggtcggga attacaccct gccctgaaga cacctatagc ataacaaaaa
     3061 aaacttgcaa ttgcaagttt tttaatcact aattagtagt agattgtata atattaattt
     3121 ttaacatcaa ttaattgaca gcgcactaat actctagcta ctcctgcctt tgtacaagta
     3181 aacaagctta agtcccaatc attgtctgat gtggcagttt tataaacttt ttcaatcgct
     3241 gttggttcaa taatttctct attaccgatt ttgtagtgat agatttgccc tgttgtagtt
     3301 gtaaaataaa catccgttcc catatctaca ttttttaaag catcaaaatg ataaggaaaa
     3361 ttatgcgcac aaatcaccat gttattagtt aaataagaac cataatacct tgtaggcgtc
     3421 ttagacagtt gttcaaaact ataattagca gctaccggta aatgcagttt taagttcgga
     3481 atatccagag ttcccaagta atctgtttta tccaactttt ttacaggtaa ttctccattt
     3541 tctgaaccct ttacttgatg cgtaatagat ttatcaagca ccttgacaat atgctgagaa
     3601 gttaaatcag cttgatgcgc ctgattaata ttataccaac cccaatagat tccagaactt
     3661 accagaataa ttccgagtat agctaaaaaa tttgctgaat atcttctaat cacgtcttct
     3721 tctccatttt aaagctatta ttaaacacag aagtcctgac ataattagta taggtattgg
     3781 ccaccatact tgtccagtaa acggaagctt tccctttgtc tgatgtgtta ctgtaggagt
     3841 aattgtctct tcttttttag gtttagcatt taaagggctc attttctcaa atgctgtaat
     3901 atcgtacttc ccatccttag gtattgatag tataaaggga gacattagtt cataaccttg
     3961 agccgtttta gtctgaataa atagataaat cccttgagga agattgttcg caacaatacc
     4021 ttcagccggt aaattatcaa acgtttgtaa aggttgagtt ttatgaacag cttttgttag
     4081 tagattgacg tatttggctt ggttactatc aaggtttact tgtgttagat catcgtcttt
     4141 tattccaata ccttgaaatg gggtagttag agtaaaaact tgattaccat caacatcttt
     4201 agcttgtgct acttggtaaa caagtaaatt accgccagcg ataccttgat tattatactt
     4261 attttgtata gtaatagaac ccgttttcat ctgatcattg gtatcagcag acacaagttg
     4321 agtacttaga ctaaataata agagaagagt tatctttagg atctttttat aaatcattgt
     4381 tctcttcctt tctcattgct tgttttaaaa ttttcttacg ttgacgtgct ctcctagtta
     4441 cttctaaaga gattaaaagt aaaatcaaag taaggaaaat agcgataaat ggtgcgatat
     4501 aaataggctc tatttgtatt gcctctgcta ctaccaaagc gttaccatta tcgtttggta
     4561 cacgatgtcc tctcactagt aaccgatggg tattaacgcc atatggtgta cacgtcacca
     4621 aagtttggta gtctttacct ttaacaattt gtaaatccct caaatcatcc ggtttaactg
     4681 ttctgatttg atccacttga taagtatatg tttcatttaa gatactgact gtccagtggt
     4741 ctccaacttt taacttatcc aaatcagaaa aaagccttga agagggtaaa cctctatgtc
     4801 ctgataaaat agaatgagtt gagtctcctc caattggaag actacttcct tctaaatgac
     4861 caatagaagt ttgaagcact ttttcacttg taccatgata aagtggtaat tttatgttta
     4921 tctttggaat tgaaatataa cccatattac ccgttttatc gatagccagt tgtgaattat
     4981 aatccaaacg ctcttggcta gtcatgtgcc acttcattcc tgaagtttta aattgcttat
     5041 tatattcttt ggctcggtta ataatttttt tataatcgtt ttcatccata tgcgttacgc
     5101 ggtcttggta atccataatc gctcgagatt ggtgaaatga attccaataa ttagcaagtg
     5161 aaggataagc cattaagcct acccccactg caattatagt gacaagcaaa atggatacta
     5221 aatgttgtct tatttttttc atatatttta aatctgtacc actttgctag cccatcttat
     5281 taagaacgta aacgacgacg agcaacaagc acgatacctg ctcctattac taaaattgca
     5341 cctataatgt agaaaattgt tgtaccaata ccacctgttg aaggcaactc agtacctttg
     5401 ttattttcaa cagttgggtt aactaaaagg ttatctgaat tagtcgtatc agtggctcca
     5461 tctcctaaaa taaccttctg agagttatct aacaaattgt aacctaaggg agcctttttc
     5521 tcaactagat agtatgtacc ttctttcaag cctgtaatgg taattatacc atctgctcct
     5581 gttgtatatt ctgttgcatt agcttctgtg ccccattcaa cgttatttgt atcgttaaag
     5641 tttagaaatt gacccgtagc attctttaaa acaaatatag caccttgtaa tgaagctttt
     5701 gtggaaccat caattttttt tatagtaatt tgaccatccc tcactgttac tttttgacct
     5761 gggtcatcat tgctagtatt ggggttgatg gtcgcaatgt ttgtattttc tggtaaatca
     5821 gctgaacctg gtttagctcc actctttaat actcctgtat aagtgactgt gattgtattt
     5881 attcccttat aaaaaaagtc atcattagct ccattttgag tatttccggt tggagtattg
     5941 gtagctgccc acggaatagt aatcgtgaaa ttattatttt cctctaacag gttatacttc
     6001 ccagttgctt tttccgaacc ttgagttaga gttgtaatat tccctgatcc atcagtaata
     6061 gttacttcat aagacccttc gttcaaatca actacagaag cagatggcat agtatccttt
     6121 ataacatatt gatacacttt ttctgtacca tgataattga ctgcattctt ataagtaata
     6181 gtatatttga ctgtatcacc aaccgagtac gttttttgat ctacagtttt tccaccacca
     6241 tctccccatg tcgcatcagt attcttttca tgaatagtag catttggagt tacagatgta
     6301 accataatta cagctccatt attaacagtg ctagaaacat aataatatcc atattgggaa
     6361 acattaataa cctcagtacc atcattattt gactcagtaa cagtggaaac tggtgtagta
     6421 ttagctgata tagatttagc ccatgtcgca atctcatttg ctgacgcagt atctttttta
     6481 gttacatatg ttctccctcc attagtagtt gtcgtaaaaa gagaattaaa atcagttgaa
     6541 gctttatact cagcttcttt accttgagga attaaataag aagctccatc tttattcgaa
     6601 tcagatacat ttgcattatc tatttctgca tcaaaaactt tatatgcttt ataggttgcg
     6661 cctttttgag tatcttgaac tgtaattgtc cctgtctcag cggcaaaagc tatcggcgta
     6721 actggtgata cagccatacc aaatgctaaa ctcgccacta acagcgattg aatcattttc
     6781 tttttcattg aaatctttct cctaaaatca tattgatgaa tgattaattc atattttttt
     6841 tcgatagtat aatattaatc ctgatggtag agctaaagct aaaccaacta ggatataaat
     6901 gtgtgttcca atacctccag tactaggcaa ttctgttcct ttactgttag taattttaaa
     6961 agtatatact gtacttccat ccactaaatt ctcttttatt ggtgtcgcat tattaccatt
     7021 ttgttcaaag gtaactcccg tagaaatcac taatactgat atatcatttt taggtagtag
     7081 gtaccctgga ggggcctttg tctctgttag gtagtatttt cctactggca aactgaggta
     7141 gttattagca tccactaata acaagccttt atcgtttgtc accagccctg aatacatagg
     7201 atgtgaagct ttattcccat tagcatctga ttcataaata tcaaaaacta cacctgctaa
     7261 aaaattatta tcattttcga cattaacttt ctgtagtcgt actttttgct tgatacgtgt
     7321 attggtaaag ctaatatcta cgtctcctga aactgtcagg gattgtaagc cggtagcatc
     7381 ataagtttta tcagcttcac cagttgctag atttttttct gtaattgact cagatacttt
     7441 aaattcatcg taggcttgtt catctattga tatagaagtt ccataaggta ctttaaattc
     7501 cttagtctga ccatctctca gcggaaaatt ctcttgttgc aacgtttcac ttggattaaa
     7561 caagaagtct ttcgtcttat cttcatctag tccaacgaca gttttactta ctctgacggt
     7621 gtattcttta ggttgccaaa cagcatataa ggtatttgtt gcatcagggt tgttatcaat
     7681 acctattgat tgacctgctg taaattccac acgtcctgta tcagctaaat ccttatcatg
     7741 atgccaacca ataaggttgt aacctgtcct tgtaaagtat tggttttcag gaattgtagt
     7801 tgtgctattc aactccatac gcggtgtctc cacttgtgtt accacattac cattttctac
     7861 tctagtacca ccgttaccat tgtatttgat ggaggtatct tctaatttga tatctcctac
     7921 tggaataatg acaggtttta tggtgatatt tttattagca tctgctaaat gggcgtcaat
     7981 atcaatggaa tcatatgggt tataaatttt accattgtac caccagccac ggaaacgata
     8041 gccatctggc attgtcggac gtctcagtaa ggctgaatgg gagccatcgt cataggaatc
     8101 agggtcagta ggctcattac ttgtttgtaa ttgctgacca gaagcatcca atgctggctt
     8161 tccatctgta ccaacagcat cattgctgta tataatatga taatctccag cctttcgcca
     8221 aatagctctt aaattgatat cttgagttac agcacctgaa aagttataag gtctaatact
     8281 accatctgga ttaacataat accaaccgac taatttgtaa gcgtctttta cgtacttgta
     8341 cttagttgtt gtatcaacat ttgagagact agtatctgtc gtataatagg catctttagt
     8401 tgagtcggga tctttatctc gtgaatcata cttataataa tatgtacctg aaggatcttg
     8461 gatataatcc cttgtaatat ctgtataatc cggaatacga tcaccataat gcaaatctaa
     8521 ataggtatca tctgtttttg ataatcggcc tccgtttgga tcaatattga cacgatatgt
     8581 taccttttgc caacctgcat agactttaac atcatgagga ggcatagtcg tgttaaagtc
     8641 aaatacttgt gtttgtgctt ggtctttata ccatttacca tcccaaacat accctggtcg
     8701 actaggttta ggttgaacag ttgtcgtatc gggggcataa gaggacaaat tttgctcata
     8761 tagaacatcc tttactggaa aattaggaag ctctgtatta tcaagcggat ctaaatattt
     8821 aatcttgtat gaattacgtt cataccatac cactaagttc aaataatctt tgtggtaggc
     8881 accatatcta tcatagtatt catctgaaat aggaactttt actcctgcac tcgtttgact
     8941 tgcgtactga tcaaaaaggt aattatcagg atataaacgt tgataatatt taacattaaa
     9001 ccctagatat ttttctgtaa aggtaaattc atctgcccca gcacctccac cggtatctgc
     9061 taaagaataa gtgccatcca aaccttgttt gaagaaccga taagtttgaa ctatattccc
     9121 ttttggaaag agtttgataa catctgaatc aacggtatca ttaggtaaaa tgaactcacc
     9181 caaataactc atcccatagg ttccaacttg gttatttcca acattggcta aataacccca
     9241 tgcacccgtc ttccattgat aaccattagc ggccaaggtt gtaccgtaaa gtcctgtgta
     9301 agtatcagca tcagagctcc aattatacga atagtaatat tctggcaagg aatatccgcc
     9361 atattttgca aattttattg tgatgagttt tctatcataa taaacattaa caacacttga
     9421 accatcgtct tttatcatga cagaagtttc tgtcctcgta ttattaactt taaagccagt
     9481 cggtagttta tcattaatat cttgttgtgt tagcgtctga ttagataaag ataggcctga
     9541 tcgtgttact tgccctgcgt actcatatgt cttttgcgca tcagtagcat ttttattatc
     9601 cgttgctgat tgttgccagt agtttatcgt gtaggttgtt tgtgcgggcg accaatgtgc
     9661 atatagcgtc gtatccttgg tcaagacttg attaaaatca aaggctgccc caccactagc
     9721 agctgtgtac caccctgcaa aagtataacc tggcctcgtt ggatcattag gcttaattgt
     9781 cgaagcaggt tggcctgtta acacacgacg aggtgcaata taggtaactc ctgttgataa
     9841 gtcgcctgtg ttgaattcaa caccgtacga ttctttaaag acaggataaa gacttatgtc
     9901 tgaagataca ggtaatgctt gaatttctga ttcagaaagt ggagctccat tttgagtttt
     9961 agaccaacct acaaataaca atgttgaagt gggtggtgaa actttaaatg aacttaaatc
    10021 cgcggttccg tttcctgaac tatccaatgg tacttgataa cgctccaaaa tacttttacc
    10081 agcagaatca tcatataaag ttactgtcgc cactttactc ataaatggac gaacacaaat
    10141 ttcttttgtc tcagttacag ttattggctc accaaattta acagggtcac catactttcc
    10201 agtagtagga tcataggtat accaaccatt aaaatgctct cctgctttaa tcgtcggaat
    10261 cccaacttct cctagagatt ctccatcttt tataatttga tgatgaactt gcatacctga
    10321 agctgtcagg aaatcataat cagttccgtc attattttga aaatggtaag ttaacctagg
    10381 aacttctgta ttatcctctt gaacaattgc ataaatggag aatgaatctg ttttaaaagc
    10441 aacatcactg ctagtgttct tagtttctgc agtatcttta gattttaata cttctgtttg
    10501 accatcatct ttaaagtgaa caactttaag gttttcatct gaagcttcta atggcttatc
    10561 atagttaacc tctactttta ctggagcttg gggttctgct tctttaccat ttgactcaat
    10621 agtaatgtca tagagtttga aatttttgat ttcactatct tgtttagcaa cctctgtcaa
    10681 tgcttttttc ttataatctt taaaagtagc tgaattgtct tttaattccg tcacctttaa
    10741 atcagcattt ttaggaatct tagcttcttt ggtcaaagtc actgttacag tatagtctgc
    10801 acctctaaac atcaatggtt cttcacgata agcagcttcc tcagaagatg atgtttctgt
    10861 tacactagaa gcaggagtct ggggcttgct ctgctcaaca cttgattgag aactagatgt
    10921 tgatgaagtt acctggctag aatttttatt ttctaaagta atccccacat catctgtctt
    10981 agtttcttca actgttattg ctggtagaat taaaaaataa gtcgttaaaa aagttgttag
    11041 gatcatcaat gaccacatga taattttcca ctctttaggg tgttttttct tttttaatga
    11101 ttcgattata aaagtttgac acttctttag catttttgca tcctccctaa ccttaattga
    11161 tactactaat cttacctaga ggccatattc tgaaagaaat tttacctaca atttgttctt
    11221 ctgaaacatc tcctacagaa gtacttcgag aatcaattga agtttttcgg ttgtctccta
    11281 atacaaaaat ttttttatca ggtacttgat atgggtattt tatattacta ttaccgagtg
    11341 ctttatgaat aacatatggt tctttcaact tatgttgatt cacgtaaaca tccccttgag
    11401 aatcaatatt aacccagtct cctgactctg caataacccg cttgactagg accttattat
    11461 tgtagtaaaa cgcgacaacg tctccatttt taaaatttga accttttact gtaaagacta
    11521 catcacctgc acttaaagtc ttattcattg aatgtccgta gattcttaat acaggcaacc
    11581 ataaaaccgc aattaaaatg gctattgagg caaccgccat caagatgtat atggtatttt
    11641 taatgacact ccaaaagcgt ttctgataag ttacgcgatc cagttcttga gataatttat
    11701 ctgaactaat ctgtcttttc attgtctacg cctctcttat ctactaaatt ctttacgttt
    11761 tctaaatagg tatctgcagc taactgagca gtctcaaaaa taccactaag cattaaggaa
    11821 gcttcggcaa tagaaccagc tttgctaatt ttgatttttt tatcatctag cgcttcttta
    11881 agttgctgaa tctctttctc ttgtttttca ataagaagtt gctgttctaa cataatttct
    11941 agcaagtctt ttctttttaa tttttttaaa tcttccatcg cgattacttc cttaactgaa
    12001 ccttaaatta tcgtttagat attatatcaa agttctaacc tttaaactca ttttttgtcc
    12061 tgtgtttttt ctcaaaaaag tctatgctaa attaacattt ttgataattt tttgaaaaat
    12121 ctcatcgaag tcattttctt tttgaaagct cgaattctag gcattaaaaa gccatatatc
    12181 aaattgatat atggcttttt ttattattta aaacaaaaga atcaatagga caatagcgtc
    12241 aatttagtga cataatctat tacagattaa gttctttttg aataatataa tccaactttt
    12301 caactgtttt ttcccatgtg aaatgttctt taattctttt agcaatattc tgttgtagtt
    12361 tctctcttaa tgccttatct tttactaata aatcaagaga ttcatggagt gactgagtat
    12421 tttcttccat gatgattcct aactcagggc tatcaataac ttcaactgtt ccaccgcgat
    12481 ctgttgcaat aatagcactc gaaagtagac cagcttctaa aatagaggtt ggtaatccct
    12541 ctggatacat tgatgggtaa acaaagatat ctgtctgtgc cattaaagac atagtctgtt
    12601 caaagtttaa tttccccaaa aagttaatct gtttggactg atatttctct ttcaaatgtg
    12661 ctaattcagg tccgtctcct gcaatctgta aataaacatt ttcagagtac tgtgacatcg
    12721 aaaatgcttc taagagcaat tcaatgcctt tttctttaat aattctacca gcataagtga
    12781 tgaaaatatc atcagcagat ttttcaaggt aagccgtgtc agcaaaatca gaacctagac
    12841 tttcagatac cgaattataa ataactcctt tagcttctat attaaaatgt tttaaccact
    12901 caacgcttct cttagatacc gcataaaaat ctggacgata gtgcttaaca cgcgctgtga
    12961 gaagatgttc atagatagct ccaaagaaat ctaaaaaacg attattgaca gaaaaatgac
    13021 ttgacccatg gtctaaaaca atactaggta aacggtgctt ctttgcaaaa gatagccctt
    13081 ctagcgttgt taactgaaaa cgtgtattac aaatcacaaa atcaatattt tcatctgaaa
    13141 catgtttcat cagcgtgttg tattctcgat ttttgttaat aataggatag cgctgcttga
    13201 caatattttt ggtcggtaaa cggtaaattt ttctaccctt gtcttcatct ataatcggta
    13261 aatcatcatg attagttgtt acaataacaa cacggtagcc acgcttaacc aaatctgctg
    13321 tcattttatc tgtataacgt tcaatacctc cgaggaaggg tagataatat cctgagaaaa
    13381 cagcaactgt ttttacctta ttttccatat ttatccactt tcatcaataa gccatctttt
    13441 aagcctttaa tcatagcaac taattttttg ctcttttgct cttctgctac caacactcga
    13501 acaaattcat ttcgcataaa tactaaatat ttgtgccgct tcttcttacc atattttttt
    13561 ataatataaa tcgcattgcg tatcatgtaa tattttcgaa atggtgaatg attcaataca
    13621 tgaaaaacat ggccaaattt tttaactcgt gaagagtgtc caatttcgtg taaaagacca
    13681 ataaaattaa cctgataagt cttatatccc atctctgaca gacgataatt catttcagag
    13741 tcaacaaaat caataaacat ctcttctgca aagccagatg tttcttcaaa aacgctcgtt
    13801 ttcattaaag cagccgaagt aatacactct tcaatttctt tatagtcaaa ttcttgcata
    13861 actaaatctt cacggttcat atcttgatac aaacaagata acataccgac cttaggtaaa
    13921 agaaggtaat tttcataatt atctatcaaa tcacctagga caaccgaatc ttgatctaaa
    13981 gtcaagaacc aatcaaattc ttgtgctact gcaaattgac cgatacagtt caaagcatat
    14041 gcaattcctt tattttctgt taaataatca acagttaggt gcccctcttc attataatcg
    14101 gccactaatt gagaaatttc ttccttattt ttcgagccat tatctacgat gtagatatgg
    14161 cttacttgag gataaattgc tcgaatgttc tgatctaagc gttcaatatt ggggttaaag
    14221 gtgacaatac ccgctaaata tttcatgttc tatgctcttt tctaaaatct ctaaataact
    14281 gaatgactgg tgctttggtt ataaaaacga taccgacata gatagttact gctactaaac
    14341 tttgaatgac ataatttacc aatgatactg acatttgagt attgatataa taaagtacag
    14401 ctccactaag agtagcagca attaaatagc gcagcattcc tcttgttaat tctttaaaag
    14461 taaatacatc tcttaaagag atagcttgat agagggagac aataaattca gtaataactg
    14521 tagagataat agctcccata gcacctaaaa ttggtattaa aagtatatta agcacaacat
    14581 ttgccacaag tccaataact gcagacattg tgtaagcttt tgtacgtctt gaagccagta
    14641 gatactgtgt ccctaaagcg ttaccataag aaatgcaaat gatcatcaaa gactcaacag
    14701 ccattagcgg accgacagct tcataccctt tcccaaaaaa gaagacggca aatgtcgacg
    14761 aaactcctac cactcccgca atcattaaca ttgagatagc attgcttaaa tcaacaagtt
    14821 tcaaaagtaa agccttagct tgcttttctt ttcctgaaga aaatagactg gagagccttg
    14881 gtaagaaaac tcctccgatt gctgaaacaa tggtaaataa aatacgaata attttatcag
    14941 attgatcaaa atatccagca ctgaccacac tatccattgc tcccagcata ctcttgttaa
    15001 gagagagata aatctgcatg gtgatttggg gtaaaaataa cattaaagtt ggtcttagat
    15061 gtattaagat atctaatcta ctaaatggta tcttgatgat ttcatgcttt aaatgatgcc
    15121 agactgttaa atttccgagt aatgatgcaa atgcaattaa aaacatataa agtgctaggt
    15181 catctggcgt cttcacaact aagaaggtca aaacaactgt aataagctta acaatggtat
    15241 tacgaatgac aattatctta aagtcttcaa ctccaataaa ataccacgaa atatcagttg
    15301 cagtcgctaa taaattaata ccttgaatta aatagaataa ttgccattcg tttgtaagta
    15361 agacaaatcc aaagaataat aacgttgcta tagatacaga ggcaagtttt aatacaacta
    15421 attcccaaaa tatcttactt ctcttatact tatttgactg cacaaaagct atctcacgat
    15481 taccgtacaa agatatccct aaagcaccaa ataaagtaaa gtacatgaca atggagctcg
    15541 tataggcatt aataccgatg ccttgaggag atagtacacg ggaaacataa ggaactgtta
    15601 ctaaaggtaa caacaaagtc aataactggt atgaggtatt ataaaacata tttttcaaaa
    15661 gtttcattag tttctcctca taattaaact aataacagta tcaaataaca ataaggcatt
    15721 tgatacaatt accaagccag ttaacgcata cggcaccact ttccttcctg cctcatttag
    15781 acgcgctgga atacttgatg caaaaacttg taccaaaagc aatgtaacag taaagtaacg
    15841 tccttgaact ccttcaatgt agcttgttgg cgctccacga tattctaaca tgataaaaaa
    15901 gacaccaaga attcccacaa agaaactagc taagaaaaga aatcccatat tttttgactt
    15961 aacaaatttt tcttcagaaa accatactag aaagaccatg agataccata ctgatgatac
    16021 ccaagtggga ataaaattct gacttggata tggagaggca agccaggaag taatgtcatt
    16081 acttaaaata ttgataccag cataattata gccaaatgtc attacataac gtcttaaaac
    16141 agcaaggaca ccaccatgtc ttgcggtcag aacataaact atagcaagtg acaaaaaggt
    16201 tagacatagc caaaatagct ttttagattt aagaccttga aataaatgtg atagcttctg
    16261 attatagccc attaatttag gattgttaac ctgcaataat accggtatta ctagcaataa
    16321 aaacagccag taattctgct tacatgcaac aatgactaaa agaatcaatc ctataagagc
    16381 aatactacga tttttagtca acgatttatc aactaaaaga tttatcaaaa gcgataaaaa
    16441 tgctccaaat acaatatatc cagtcgagtc ataagataga ctagcaaatg aatttagaac
    16501 tacgggactg aggaaaatag caaagtagat taactttcct tttttcacat accggataat
    16561 gagggcaacc aaggtgattg agacaaagga actaaataac cttgctactg taatcattac
    16621 ccccatagaa ggatacacaa atttgcctaa aaataagcca actgctggca caagatgacc
    16681 ccaaaaaaca aagttatgtt tagaatagtt tatctcacga ggcaaatgat taccatcaac
    16741 aaaattggct ttatttaaat agtatttttc aaatctagta ccatctctat aggaggtcga
    16801 ttgtcgtctc atacctgaat caatattata ttctccatag taagaagtat caataatgtt
    16861 atttgaaata cgaccagaca cagctaaatg gtattgtcca tcaggttcat tgaaaaatgg
    16921 cattaatagg ctcaatacaa tagtaatcgt taagcctaat ataaaatatg ttttatgcaa
    16981 gttcttgtct agaaaaaact tttcaaatag acgaactttt ttgaggttca tattaactcc
    17041 tttttatatc ttttaataaa aacaccaagt cacgaatgcg atttgaaaag ctatcataga
    17101 accgtttttt agtcatcatt tgtttaagta ttgctacaaa atctttattt tgagcatttt
    17161 tataacgttg ctgattaaaa tccagataag ctttagcttc ccttaattga cta
//
LOCUS       DATRUK010000042        17073 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.042, whole genome shotgun sequence.
ACCESSION   DATRUK010000042 DATRUK010000000
VERSION     DATRUK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17073
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.042"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..1177
                     /locus_tag="VBE34_001340"
     CDS             <1..1177
                     /locus_tag="VBE34_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEO7264056.1"
                     /translation="QIDYAVVIPVFFLLMIGLASIYVATMNDYPSNIYIAMFQQVSWI
                     IMGCIIAFVVMLFSTEFLWKATPYLYALGLTLMVLPLIFYSPQLFAATGAKNWVTIGS
                     VTLFQPSEFMKISYILMLSRITVSFHQKNRKTFQDDWKLLGLFGLVTLPVMILLMLQK
                     DLGTALVFLAILSGLILLSGISWWIILPILSTIVLFIASFLMIFISPNGKEWFYNLGM
                     DTYQINRLSAWIDPFSFADSIAYQQTQGMVSIGSGGVTGKGFNILELSVPVRESDMIF
                     TVIAENFGFIGSAIVLGLYLIIIYRMLRITIESNNQFYTFISTGFIMMIVFHVFENIG
                     AAVGILPLTGIPLPFISQGGSSLLSNLIGIGLVLSMSYQNTVRQAKANGSHLKYRSI"
     gene            1296..1856
                     /locus_tag="VBE34_001341"
     CDS             1296..1856
                     /locus_tag="VBE34_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEO7264057.1"
                     /translation="MNYHDYIWDLGGTLLDNYESSTRAFVETLKEFGYQADHDSVYQK
                     LKESTADAIAYFIPEEADFLKEYKANEAKVLETPILFQGAKELLAKIQRQGSRNFLVS
                     HRDNQVIVILEKTEIIDYFTEVVTADNGFSRKPSPESMLYLKEKYQIDNCLVIGDRDI
                     DKQAGESAGFDTLLVDGSKSLMEIIE"
     gene            1857..3809
                     /gene="gyrB"
                     /locus_tag="VBE34_001342"
     CDS             1857..3809
                     /gene="gyrB"
                     /locus_tag="VBE34_001342"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEO7264058.1"
                     /translation="MTEETKNMEQRAQEYDASQIQVLEGLEAVRMRPGMYIGSTSKEG
                     LHHLVWEIVDNSIDEALAGFAGHIKVYIEPDNSITVVDDGRGIPVDIQEKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKNGKVHYQEYQRGVVV
                     NDLEIIGDTDLSGTTVHFTPDPEIFTETTVFDFDKLAKRIQELAFLNRGLRISISDKR
                     EGQEVEKEYHYEGGIGSYVEFINENKEVIFENPIYTDGELDGISVEVAMQYTTGYQET
                     VMSFANNIHTHEGGTHEQGFRTALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFNRFLLENPQVAKKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNNAEMNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKVRYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKAYIQPGVNQEEE
                     LRQALDTYSSGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVSVDDAAEADKIF
                     DMLMGDRVEPRREFIEANAVYSNLDI"
     gene            3903..5627
                     /gene="ezrA"
                     /locus_tag="VBE34_001343"
     CDS             3903..5627
                     /gene="ezrA"
                     /locus_tag="VBE34_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEO7264059.1"
                     /translation="MSSGIILLLVAIVLLVIIAYVVGVVIRKRNDTLIANLETRKQEL
                     VDLPVQEEIEQVKLLHLIGQSQSTFREWNQKWTDLSTNSFKDIDFHLVEAENLNDSFN
                     FVRAKHEIDNVDSQLTIIEEDIVSIREALEVLKEQEEKNSARVTHALDLYETLQKSIS
                     EKEDNYGTTMPEIEKQLKNIEAEFSHFVTLNSTGDPIEASEVLNKAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKDIEQRFQEVREAIRSNSDGLV
                     SLDLDRARDENEHIQEKIDKLYDIFEREIAAYKVAHKDSKIIPQFLAHAKSNNEQLGH
                     EIKRLSAKYILNENESLSLRSFTNDLEEIETKVLPSVENFGQEASPYTHLQILFERTL
                     KTLTTVEENQMEVFEAVKTIESVETRARQNMDKYVNKLHMIKRFMEKRNLPGIPQDFL
                     STFFTTSSQIEALINELSRGRIDIEAVSRLNDVTTNAIANLEQATYLVVQDATLTEQL
                     LQYSNRYRSFEQNVQKSFEQALYLFEVEHNYKASFDEISYALETVEPGVTDRFVTSYE
                     KTQERIRF"
     gene            5721..6362
                     /gene="serB"
                     /locus_tag="VBE34_001344"
     CDS             5721..6362
                     /gene="serB"
                     /locus_tag="VBE34_001344"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEO7264060.1"
                     /translation="MIKGLLVMDVDSTLIMEEAIDLLAIEAGVGKQVAALTDAAMRGE
                     LDFEEALKKRVALLKGLPVTILTDILSSIHFTPGAYELIKECHKRQMKVGLVSGGFHE
                     TIDILAKQLQVDYVKANRLGVKGGFLTGEVEGEIVTKEVKKIKLKEWASENHLDLSQT
                     IAMGDGANDLPMIKSAGVGIAFCAKPIVREEAAYQINEKNLLKVFDIIDANEN"
     gene            complement(6383..6868)
                     /locus_tag="VBE34_001345"
     CDS             complement(6383..6868)
                     /locus_tag="VBE34_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO7264061.1"
                     /translation="MEIWDAYDQYGFITGLTLNRDQNIPQGLFHLVVDVILFHEDGDV
                     LMMKRHPKKKAFPAYFEATAGGSALKGENAKQAILRELKEETGIVPQCLTFLNREWFS
                     ERSYFVDHFIAKYNGAKDIITLQEGETVDYIWLKPEYIDLFLSKNKLIPSQIKLLKSL
                     I"
     gene            complement(6881..7336)
                     /locus_tag="VBE34_001346"
     CDS             complement(6881..7336)
                     /locus_tag="VBE34_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEO7264062.1"
                     /translation="MTELDNKILQKASGENRLDPDQQRYYLGTYKERVVLHLSLKEAQ
                     EEKFQLAITSALNNLILEYPNLSLKLSPNLPTDVQMFYMKLANRLSLAATIVNEANAN
                     SPFGIIIHSDKAENVEWNDFETQFPDLFNSPKKEESPKKSLWQHFFSQK"
     gene            7534..8841
                     /gene="eno"
                     /locus_tag="VBE34_001347"
     CDS             7534..8841
                     /gene="eno"
                     /locus_tag="VBE34_001347"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEO7264063.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNVIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEALRWGAEVFHALKKILKERGLETAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDAERKVYDYSKFEGEGGAVRTAAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGGRVQLVGDDFFVTNTDYLARGIK
                     EEAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            complement(8949..9923)
                     /locus_tag="VBE34_001348"
     CDS             complement(8949..9923)
                     /locus_tag="VBE34_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO7264064.1"
                     /translation="MKRKQFIKLGIATLLTVISLYTPINLATNHTTENIVTAQEYKTK
                     ENGTLPFKHKRQLVLGELDDKGRATFAHIQLKVKDEPKKKRVKRLKTTPVGWHNFKFY
                     YNDGTQKAWLMSRGRLICHQFSGLNNERKNLVPMTNWLNTGNYNSTNSSNPESMLFYE
                     KQLKTWLSTHKNYYLDYKVTPIYQNNELIPRKIELKYVGIDKTGKLLPIFIGNKSTQD
                     QFGISTVTLENTSPNATIDYLSGKAQNTVLSAKEQRKLIAKHEEEKRLAEKKVEEEKA
                     AAETQKKQETLVQEQTSQGYKRDYRGRWHRPNGQYASKAEIAAAGLQW"
     gene            10152..11435
                     /gene="aroA"
                     /locus_tag="VBE34_001349"
     CDS             10152..11435
                     /gene="aroA"
                     /locus_tag="VBE34_001349"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEO7264065.1"
                     /translation="MKLLTNANTLKGTIRVPGDKSISHRAIIFGSISQGVTRIVDVLR
                     GEDVLSTIEAFKQMGVLIEDDGEIITIYGKGFAGLTQPNNLLDMGNSGTSMRLIAGVL
                     AGQEFEVTMVGDNSLSKRPMDRIALPLSKMGARISGVTNRDLPPLKLQGTKKLKPIFY
                     HLPVASAQVKSALIFAALQTKGESLIVEKEQTRNHTEDMIRQFGGHLDIKDKEIRLNG
                     GQSLVGQDIRVPGDISSAAFWIVAGLIIPNSHIILENVGINETRTGILDVVSKMGGKI
                     KLSSVDNQVKSATLTVDYSHLQATHISGAMIPRLIDELPIIALLATQAQGTTVIADAQ
                     ELKVKETDRIQVVVESLKQMGADITATADGMIIRGNTPLHAASLDCHGDHRIGMMIAI
                     AALLVKEGEVDLSGEEAINTSYPNFLEHLEGLVNA"
     gene            11428..11940
                     /locus_tag="VBE34_001350"
     CDS             11428..11940
                     /locus_tag="VBE34_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEO7264066.1"
                     /translation="MPKVLLGFMGVGKTSVANCLENEVIDMDSLIEKHIGMSISRFFT
                     EEGEASFRALESQFLNELLKKKNEGLVIASGGGIVLLEENRRLLTLNRHNNILLTGSF
                     EVLYHRIKKDEKNRRPLFLNHSKEEFYDIYQKRMLLYSGLSDMIIDTDYLTPQKIATV
                     IGESLVNLSN"
     gene            11997..13370
                     /locus_tag="VBE34_001351"
     CDS             11997..13370
                     /locus_tag="VBE34_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO7264067.1"
                     /translation="MSRNNYGQLNHHEELRYNYLLKNIHYLNEREKMEFQYLHYKKTA
                     VRPQRRTESPPTNSYYEEPYSDSYYQDDDFYSEPQLTSQGLPIYQEERAPKKKKQRAR
                     KEKQRVKVMAPFPPKAITPPRKKKKFKGFLKFIGIILLIVLSGMVFMFVKGMRDVNNG
                     KSHYSPAIIEDFKGKDAVDGTNILILGSDKRVSERSTDARTDTIMVANVGNKDNKVKM
                     VSFMRDLLVNIPNYSTEGYYDMKLNASFNLGEQDNHKGAEYVRQTLKNHFDIDIKYYV
                     MVDFETFADAIDTLFPNGVKINAKFGLVGGQSADSVKVPDDLRMKNGVVPSQKIKVGI
                     QYMDGRTLLNYARFRKDDDGDFGRTQRQQQVMRAIVSQIKDPRRLFTGSAAIGKAYAL
                     TSSNLSYSFVLTDGIPILSDAKNGIKQMTIPREGDWVDDYDQYGGQGLTIDFAKYKKI
                     LKKMGLR"
     gene            13471..14826
                     /gene="rlmD"
                     /locus_tag="VBE34_001352"
     CDS             13471..14826
                     /gene="rlmD"
                     /locus_tag="VBE34_001352"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO7264068.1"
                     /translation="MLHKNDIIETEISDISHEGMGIAKVDGFVFFVENALPGEIIKMR
                     VLKLRKRIGYGKVEEYLTTSPHRNEGLDYTYLRTGIADLGHLTYEQQLLFKQKQVADN
                     LYKIAHISDVLVEPTLGMTIPLAYRNKAQVPVRRVDGQLETGFFRKNSHTLVSIEDYL
                     IQEKEIDALINFTRDLLRKFDVKPYDEEQQSGLIRNLVVRRGHYTGQLMLVLVTTRPK
                     IFRIDQMIEKLVSAFPSVVSIMQNINDRNSNVIFGKEFRTLYGSDTIEDQMLGNTYAI
                     SAQSFYQVNTEMAEKLYQKAIDFSDLNSEDIVIDAYSGIGTIGLSVAKQVKHVYGVEV
                     VEKAVSDAKENATRNGITNSTYVADSAENAMAKWLKEGIKPTVIMVDPPRKGLTESFV
                     YSAAQTKADKITYISCNSATMARDIKLFEELGYHLVKIQPVDLFPMTHHVECVALLVK
                     A"
     gene            14934..15155
                     /locus_tag="VBE34_001353"
     CDS             14934..15155
                     /locus_tag="VBE34_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264069.1"
                     /translation="MPTMQLLDELVLPKDITIADVSQSIQEFEIERDALQEFFDDMLK
                     TYTTYQEMKDYIKSREANTPKQETEEKSL"
     gene            15273..16010
                     /gene="aphA"
                     /locus_tag="VBE34_001354"
     CDS             15273..16010
                     /gene="aphA"
                     /locus_tag="VBE34_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEO7264070.1"
                     /translation="MKKVLVSSLLVLGITITLQTVVEAKGPKVAYTQEGMTALSDTNK
                     DKVTTISIDEIQKSLEGKKPITVSFDIDDTLLFSSQYFQYGKEYVTPGSFDFLHKQKF
                     WDLVAKRGDQDSIPKEYAKKLIAMHQKRGDKIVFITGRTRGSMYKEGEVDKTAKALAK
                     DFKLDKPIAVNYTGDKPKKPYKYDKSYYIKKYGSDIHYGDSDDDIHAAREAGARPIRI
                     LRAPNSTNLPLPEAGGYGEEVLENSAY"
     gene            16330..16847
                     /locus_tag="VBE34_001355"
                     /pseudo
     CDS             16330..16847
                     /locus_tag="VBE34_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
     gene            complement(16919..>17073)
                     /locus_tag="VBE34_001356"
     CDS             complement(16919..>17073)
                     /locus_tag="VBE34_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001875973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEO7264071.1"
                     /translation="QLETAYQVPYSFALGIYISTIESIILNWIDHDFTEEPEEIARYI
                     TSVVRI"
BASE COUNT         5676 a         2597 c         3370 g         5430 t
ORIGIN      
        1 gcaaattgat tatgctgttg tgataccagt atttttttta ttaatgattg gtttagcctc
       61 aatttatgtt gcaacgatga atgattatcc ttctaatatc tatatcgcga tgtttcagca
      121 ggtgtcttgg attattatgg ggtgtattat tgcatttgtc gttatgcttt tcagtactga
      181 atttttatgg aaagccacgc cctatctgta tgctttagga ctaaccctaa tggttcttcc
      241 cttaattttt tatagtccac agctttttgc ggcaacaggt gctaaaaatt gggtgaccat
      301 tggctcggta actttgtttc aaccgtcaga gtttatgaag atttcctata ttttaatgtt
      361 atctcgaata actgttagtt ttcatcagaa aaatcgcaaa acttttcaag atgattggaa
      421 gttgctagga ttgtttggtt tggttacatt gccagtgatg attcttctaa tgttacaaaa
      481 agatttagga acagcattag ttttccttgc cattttgtca ggtttaatcc ttttatcggg
      541 catatcttgg tggataatct tgccaatttt atctactatc gttcttttta tagctagctt
      601 tttgatgatt tttatctcac caaacggcaa ggaatggttt tataatttgg ggatggatac
      661 atatcaaatc aaccgtttat ctgcttggat tgatccattt agttttgcag actcaattgc
      721 ttatcaacaa acacagggta tggtatcaat tggaagtggc ggggtaacag gtaaaggttt
      781 taatatttta gagctctcgg ttcctgttcg tgaaagtgac atgattttta ctgtcattgc
      841 tgagaatttt ggcttcattg gttcggcgat tgttttaggg ttatatctca ttatcattta
      901 ccgtatgtta aggatcacca ttgaatctaa taatcaattt tatactttta tatcaactgg
      961 ttttatcatg atgattgttt tccatgtttt tgaaaatatt ggtgcagcag ttggtatatt
     1021 acctctaaca ggaatcccac ttccgtttat ttcgcaaggt ggaagttcat tactaagtaa
     1081 tcttattgga attggattgg tcttatccat gtcttatcaa aatacagttc gtcaggccaa
     1141 ggctaatgga tcacatttga agtatcgtag tatataacaa aaaaaggttg gaaagataat
     1201 ccagcctttt aataatcgaa atattttaga ggagtgagtt aattaagtaa ggtggtagct
     1261 ataaaaaatc gtaataatat gatataataa agggtatgaa ttatcatgat tatatttggg
     1321 atttgggtgg tacactactt gataattacg aaagttccac acgtgctttt gtagagactt
     1381 tgaaagaatt tggatatcaa gcagaccacg atagtgtgta tcaaaaatta aaagagtcaa
     1441 cagcagatgc aattgcatat tttatccctg aagaagctga ttttttaaaa gagtataagg
     1501 ctaatgaagc taaggttttg gaaacaccaa ttctattcca aggggcaaaa gaacttttag
     1561 cgaaaattca aagacaaggt agtcgtaatt ttttggtgtc tcatcgtgat aatcaagtta
     1621 tagttattct ggaaaaaaca gaaattattg actattttac agaagtagtg actgctgata
     1681 acggcttttc aaggaaacct agtcccgaga gtatgcttta tttgaaagaa aaataccaaa
     1741 ttgataattg tttagtaata ggcgatcgtg atatagataa gcaagcagga gaatcggctg
     1801 gttttgatac cttattagtc gatgggtcaa aatcattgat ggagataata gagtaaatga
     1861 cagaagaaac aaagaatatg gaacaacgtg ctcaagaata tgacgcaagt cagattcaag
     1921 tattagaagg tttagaagct gttcgcatgc gtccaggcat gtatatcggt tcgacttcga
     1981 aagaagggct tcatcacctt gtatgggaaa ttgttgataa ctcaattgat gaagccttag
     2041 ctggatttgc aggacatatt aaagtttaca ttgaaccaga taactctatt acagttgttg
     2101 atgatggtcg cggtattccg gtagatatcc aagaaaaaac tggaagacca gctgtagaga
     2161 cagtttttac agttctccat gccggtggta aatttggtgg tggcggatac aaggtttctg
     2221 gtggcttaca tggtgtaggg tcatctgttg ttaatgcatt gtcaacacaa ttagatgtca
     2281 aggtttataa aaatggcaag gttcattatc aagaatacca acgcggtgtt gttgttaatg
     2341 atcttgaaat tatcggtgat actgacttat caggaacaac agttcacttt acgcctgacc
     2401 cagaaatttt cactgaaacc actgtatttg attttgataa attagccaaa cgtattcagg
     2461 aattagcttt tttaaatcgt ggactaagaa tctccatttc agacaagcga gaaggtcaag
     2521 aagttgaaaa agaatatcac tatgaaggtg gtataggttc atacgttgag tttatcaatg
     2581 agaacaaaga agtgattttt gaaaatccta tttatactga cggtgaatta gatggtattt
     2641 cagttgaagt agcaatgcag tatactactg gatatcaaga gactgttatg agttttgcaa
     2701 ataacatcca cactcatgaa ggaggaacgc acgaacaagg atttcgtact gccttgacac
     2761 gtgtgattaa tgattacgct aaaaagaata agattctcaa agaaaatgaa gataatttaa
     2821 ctggagaaga tgttcgtgaa ggtttaacag cagttatatc tgttaaacat ccaaatccac
     2881 aatttgaagg acaaactaaa acaaaacttg gaaattctga agttgttaaa attacaaatc
     2941 gtctctttag cgaagctttc aatcgattcc tattagaaaa tccacaagta gctaagaaga
     3001 ttgttgaaaa aggaattttg gcatctaaag cccgtattgc agcaaaacgg gcgcgtgaag
     3061 taacacgtaa aaagtcaggc ttagagattt caaacttacc tgggaaactg gctgattgct
     3121 catctaataa tgctgagatg aatgaacttt ttatagtgga gggagattct gcgggagggt
     3181 ctgcaaaatc aggacgtaat cgtgagtttc aagcaatttt accgattagg ggtaagattc
     3241 taaacgttga aaaagctaca atggataaaa ttttagcaaa cgaagaaatc cgaagtctct
     3301 tcacagctat gggaactgga tttggtgcag attttgatgt atcaaaagtc agatatcaaa
     3361 aattagtcat tatgactgat gccgatgttg atggagctca catcagaact ttacttttaa
     3421 ctctgattta tcgttttatg cgacctgttt tggaagcagg atatgtttat atcgctcaac
     3481 caccaatcta tggtgtcaaa gtaggaagtg agattaaagc ttatattcag ccaggggtca
     3541 atcaagaaga ggaattacgt caagctctag atacctatag ttcaggacgt tcaaaaccta
     3601 ctgtacaacg ttacaaaggt ctaggtgaga tggatgacca tcaactttgg gaaacaacaa
     3661 tggatcctga aaaccgcttg atggcacgtg tctcagttga tgatgcagca gaagcagata
     3721 agattttcga tatgctaatg ggagatcgag tagaaccacg acgtgaattc attgaagcta
     3781 atgctgttta tagtaactta gatatctagt ccaaagatag ctttgaaaaa cagttcaagt
     3841 atgctataat cttaacgtta tagtagattt ttagaaaatt agaccattat aaggagagtt
     3901 gaatgtcgag cggaatcata ttgctgcttg tggctatagt cctgcttgtg attattgcct
     3961 acgtagtcgg cgtcgtaata agaaaacgta atgatacatt aattgcaaac ttagaaacta
     4021 gaaaacaaga gttagttgac cttcctgtgc aagaggagat tgagcaagtc aaattgcttc
     4081 atttgattgg ccaaagtcaa tcaactttta gagaatggaa ccaaaaatgg actgatttat
     4141 caactaattc atttaaagat attgattttc atttagttga agctgaaaat cttaatgatt
     4201 catttaattt tgtgcgtgct aagcacgaga ttgataatgt tgatagtcag ctaactatta
     4261 tagaagaaga tattgtttcg attcgtgaag ctttagaagt tttaaaagag caagaagaga
     4321 aaaatagtgc ccgtgtaaca catgctttgg acctatacga aacacttcaa aaatcaattt
     4381 ctgaaaaaga agataattac gggacaacca tgccagaaat tgaaaagcag ttgaaaaata
     4441 tcgaggctga attttcacat tttgtgacct taaactcaac tggtgacccg attgaagctt
     4501 ctgaagtttt gaataaagca gaggagcata cgattgcttt agggcaaatt acagaacaaa
     4561 ttccagctat tgttgctaaa ttagaagacg attttccaga tcagttagat gatttggaga
     4621 caggatatcg tcgtctttta gaggaaaact accatttccc tgaaaaggat attgaacaac
     4681 gttttcaaga agttcgtgag gctattcgtt cgaattcaga tggtttagtt agtttggatc
     4741 tagatcgtgc tcgcgatgaa aatgaacata ttcaagaaaa aattgataaa ctttatgata
     4801 tttttgaacg tgagattgct gcttataaag tagctcataa ggatagtaaa attattcctc
     4861 aattccttgc tcatgcaaaa tctaacaatg aacaactggg gcatgaaatc aaacgattat
     4921 ctgctaaata tatcttaaat gaaaatgaaa gcttaagttt acgttccttc acaaatgatt
     4981 tggaagaaat tgaaacaaag gttttacctt ctgtagaaaa ttttggtcaa gaagcttcac
     5041 cttatactca tctacaaatt ttatttgagc gtacgttgaa aacattgact actgttgaag
     5101 aaaatcaaat ggaagttttt gaagccgtta aaactattga aagcgttgag acacgagcac
     5161 gccaaaatat ggataagtat gttaacaagc tccatatgat taaacgtttc atggaaaaac
     5221 gcaatcttcc aggaatcccg caagacttct taagcacttt ctttacaaca agttctcaaa
     5281 ttgaggcact tattaatgaa ctcagtcgcg gtcgtattga tattgaggct gtttcacgtt
     5341 taaatgatgt gactacaaat gctatcgcta atttagagca agctacatat ctagttgttc
     5401 aagatgcgac tctcacagag cagttactac agtattcaaa ccgttatcgt agttttgaac
     5461 aaaatgtgca aaagagcttt gaacaagctt tgtacttatt tgaagttgaa cataactata
     5521 aagcatcttt cgatgaaatt tcatacgcac ttgagactgt ggaaccaggt gtaactgacc
     5581 gttttgtgac gtcttatgaa aagacacaag aacgtattcg tttctaaaaa agaagagtta
     5641 gtactcttct ttttttaatt gctatctatg aaaggtgcta attttaaggt acaatagtaa
     5701 aaaagttttg gaggttttaa atgattaaag gtttgttagt catggacgtt gattcaacct
     5761 taataatgga agaagctatt gatttattag ctattgaagc aggggtgggg aaacaggttg
     5821 ctgcactgac tgacgctgca atgcgtggcg agttagattt tgaagaagct ttgaagaaaa
     5881 gagtagcgct acttaaaggt ttgcctgtta ctattttgac agatatatta tcctcaatac
     5941 attttactcc aggggcttat gaattgatta aagagtgtca caagcgtcag atgaaagttg
     6001 gtcttgtatc aggaggtttt cacgagacga ttgatatctt agctaagcaa ttacaagttg
     6061 attatgtcaa agcgaatcgt cttggtgtca aaggtggttt tctcactggc gaagttgagg
     6121 gagagattgt cactaaagaa gtgaaaaaaa taaagttaaa agagtgggct tctgaaaatc
     6181 acttagacct ttcacaaacc atcgcaatgg gagatggtgc caatgattta ccaatgatca
     6241 aatccgctgg ggtaggaatt gctttttgtg caaaaccaat tgtcagagaa gaagctgcgt
     6301 atcagattaa tgaaaaaaat ttgcttaagg tatttgatat tattgatgca aatgaaaact
     6361 aggcctttat taagcctagt ttttagatta aggattttaa gagttttatt tgagagggta
     6421 ttaatttgtt tttagataaa aataaatcaa tatattctgg ttttaaccag atatagtcaa
     6481 cggtctctcc ttcttgcaat gtaattatat cttttgcacc attgtatttt gcgataaaat
     6541 ggtctacaaa gtaagaacgc tcagagaacc actctctatt aagaaaggtt agacattgag
     6601 gaacgatacc agtttcttct ttgagttccc ttagaatagc ttgcttagca ttttctcctt
     6661 taagggcaga tccgccagcg gtggcttcga aataagcagg aaaagctttc tttttagggt
     6721 gccgtttcat catcaatacg tcaccatcct catgaaaaag gataacatca acaactaagt
     6781 ggaataagcc ttgtgggata ttttgatccc tattaagtgt aagacctgta atgaacccat
     6841 attggtcata agcatcccaa atttccatta gacctccttg ttatttttga ctaaagaaat
     6901 gttgccatag acttttttta ggagactcct ctttttttgg tgaattgaat aaatcaggga
     6961 attgtgtctc aaaatcgttc cattcaacgt tttcagcctt atcactatgt ataataatac
     7021 caaatggaga attggcattt gcttcattaa caatcgtagc agctagtgat agacggttag
     7081 ctaacttcat gtagaacatt tgaacatcag tagggagatt tggtgataat tttaaactaa
     7141 gattaggata ttcaagtatc agattattaa gagcagatgt tatagcaagt tgaaactttt
     7201 cttcttgtgc ctcttttaaa cttaagtgga gcacaactct ttctttataa gtaccaaggt
     7261 agtaacgttg ttgatcagga tcaagacgat tttcaccgct tgctttttgt aggattttat
     7321 tgtcaagttc agtcataatt tctccttgaa tttgctaatt ttattataac ctaaatgggt
     7381 tgcattagca caggtaaact tgaaaagaat tagtgatttt tttatcaaaa accacgtaat
     7441 aaagcggttt cactgggttt ttccttgtta ttaactaaag attatgatat tatgaatatg
     7501 taaaaaatta aaactcataa ggagagtatt gtaatgtcaa ttattactga tgtttacgca
     7561 cgcgaagtcc ttgactcacg cggtaaccca acacttgaag tagaagttta tacagaatca
     7621 ggtgctttcg gacgtggtat ggttccttca ggagcttcta ctggtgaaca cgaagcagtt
     7681 gaacttcgcg acggcgacaa gtctcgttac ggtggtcttg gaacacaaaa agctgttgac
     7741 aatgtaaata acgttattgc agaagcaatc attggttatg atgtacgcga ccaacaagct
     7801 atcgaccgtg ctatgatcgc tcttgacgga actcctaata aaggtaaatt aggtgcaaat
     7861 gctattcttg gtgtttctat tgccgtagca cgtgctgcag ctgattacct tgaagtacca
     7921 ctttacagct accttggtgg attcaacact aaagttcttc caactccaat gatgaacatc
     7981 atcaacggtg gttctcactc agacgctcca atcgcattcc aagaattcat gattatgcca
     8041 gttggtgcac caactttcaa agaagcactt cgttggggtg ctgaagtatt ccatgctctt
     8101 aagaaaatcc ttaaagaacg tggtcttgaa acagctgttg gtgatgaagg tggatttgca
     8161 cctaaatttg aaggtactga agatggcgta gaaactattc ttaaagctat tgaagcagct
     8221 ggttacgaag ctggcgaaaa cggaatcatg attggttttg actgtgcttc atcagagttc
     8281 tacgatgcag aacgtaaagt atacgactac agtaaattcg aaggtgaagg tggagcagtt
     8341 cgtactgctg cagaacaaat cgattacctt gaagagttgg ttaacaaata cccaatcatc
     8401 actattgaag atggtatgga tgaaaacgac tgggatggtt ggaaagctct tacagaacgc
     8461 cttggtggac gtgtacaatt agttggtgac gatttcttcg ttacaaatac tgattactta
     8521 gcacgtggta tcaaagaaga agctgctaac tcaatcctta tcaaagttaa ccaaatcggt
     8581 actttgacag aaactttcga agctattgaa atggctaaag aagctggtta cactgcagtt
     8641 gtatcacacc gttcaggtga aactgaagat tcaacaatcg ctgatatcgc agttgcaact
     8701 aacgctggtc aaattaaaac aggttcatta tcacgtactg accgtattgc taaatacaac
     8761 caattacttc gtatcgaaga ccaacttggt gaagttgctc aatacaaagg tatcaaatca
     8821 ttctacaact taaaaaaata gtctcagatt attaaacagt tgaaaatata ataaaaacca
     8881 tcttgcatat gcaagatggt ttttatgtac acattataat aatagaattg atttgataat
     8941 aaacagttct accattgcaa tccagcagca gcgatttctg cttttgaagc gtattgtcca
     9001 tttggacgat gccaccttcc tctatagtca cgtttgtagc cttgactagt ttgttcttgt
     9061 actagagttt cttgtttttt ctgagtttca gcagcagctt tttcctcttc aactttcttt
     9121 tctgcaagtc gtttttcttc ttcgtgctta gcgataagtt ttctttgttc tttagctgat
     9181 aagacagtat tttgagcctt tccgctgagg tagtcaatgg tagcatttgg tgaggtattc
     9241 tctaaggtaa cagtgctgat gccgaattga tcttgagtgg atttgtttcc gataaaaata
     9301 ggtaataatt tcccagtctt atcaatacca acatatttta attcaatttt tcttggaatt
     9361 agttcattat tttgataaat tggtgttact ttataatcta agtagtagtt tttatgagtc
     9421 gaaagccaag tctttagttg tttttcatag aatagcatgc tctcagggtt agatgaattc
     9481 gtgctattgt agtttccagt atttagccaa ttcgtcattg gaactaaatt ctttctttca
     9541 ttgtttagtc cactaaattg gtggcaaata agacggcctc ggctcattag ccaagctttt
     9601 tgagtaccat cattataata gaatttaaaa ttgtgccatc caacaggggt tgtttttaaa
     9661 cgttttactc tctttttctt gggttcatct ttaactttta gctgaatatg tgcgaatgtg
     9721 gcacgacctt tgtcatccaa ctctcctaaa accaattgtc gtttgtgttt aaaaggtaaa
     9781 gtaccattct cttttgtttt atactcttga gcagtaacaa tattttctgt ggtatgattt
     9841 gtagctaggt ttattggtgt gtaaagcgaa ataaccgtta gtaaggttgc aattcctaat
     9901 tttataaact gttttctttt catctgttaa cctctaaaat aacataaaat agtagctgtt
     9961 tatttttaaa tgcattctga aaattaaaat gtgatttttg taacaatatt ataacataat
    10021 gatgtatgac atgtgaagtt aatttataaa agatgttaca gctatataga ggagctatag
    10081 tataaataaa aaattcaaaa tatagcatat attatgatac aataatagca aaatagattt
    10141 tgggagttat catgaaatta ctgactaatg caaatactct caagggaacc atccgtgttc
    10201 ctggcgataa atcaataagt cacagggcta ttatttttgg gagtatatct cagggagtta
    10261 cccgaattgt tgatgtctta agaggcgagg atgttttatc aactatagag gcttttaaac
    10321 aaatgggggt tctaattgaa gacgatggcg agataatcac tatctatggc aaaggatttg
    10381 caggtttaac acaaccgaat aatctcttag acatgggaaa ctctggtact tcaatgcgtc
    10441 tgatagcagg tgtgttagct ggtcaagagt ttgaagtgac gatggttgga gacaatagtt
    10501 tgtcaaagag acctatggat cgtatagcat tgcctttaag caaaatggga gctagaattt
    10561 cgggagtaac taaccgtgat ttaccaccat taaaacttca aggtacaaaa aaattaaaac
    10621 ctatttttta ccatttaccc gtagcttctg cacaagtaaa atcagcttta atttttgcgg
    10681 ctttacaaac taagggagaa tctctcattg tagaaaaaga gcaaacaagg aatcatactg
    10741 aggacatgat tcggcaattt ggtggacatt tggatattaa agataaagaa attcgactca
    10801 atggcggtca gagcttagta ggacaagata taagagttcc tggtgatatt tcgagtgctg
    10861 ccttttggat agtagcgggt cttattattc ctaacagtca tattatttta gaaaacgtag
    10921 gtataaacga gacacgaact ggaatccttg atgtcgtcag taaaatgggt ggaaaaatta
    10981 agttatcttc ggttgataat caagtaaaat cagcaaccct tacagttgat tactctcatc
    11041 tacaagctac tcatatttct ggggctatga ttccacgtct gattgatgaa ttacctatta
    11101 ttgctctttt agcaacacaa gctcaaggca ccacagtcat agcagatgct caggaattaa
    11161 aggtgaaaga gacagatcgg attcaggtcg ttgtcgaatc gctaaaacag atgggtgctg
    11221 atattactgc tacagcagat ggtatgatta tacgtggtaa tacccctctt cacgctgctt
    11281 cacttgattg tcatggagat catagaatag gaatgatgat agctattgct gctctcttgg
    11341 tcaaagaagg cgaagtagat ttatctggtg aagaagctat taatacaagc tatcctaact
    11401 ttttagaaca tttggaggga ttagttaatg cctaaggtat tattgggatt catgggagtt
    11461 ggcaagacta gtgttgccaa ctgcttagaa aatgaagtaa ttgatatgga tagtctgatt
    11521 gaaaaacata ttggtatgtc aatttcccga ttttttacag aagaaggaga agcctccttt
    11581 cgtgctttag aatctcaatt tttaaacgaa cttttaaaga aaaaaaatga aggacttgtg
    11641 atagctagtg gaggaggtat tgtactcctt gaagaaaatc gtcgtctttt aactttaaat
    11701 cgtcataata atattctttt aacaggaagt tttgaagtct tatatcatcg tataaaaaaa
    11761 gatgagaaga atagaagacc attattttta aaccattcaa aagaagaatt ttatgatatt
    11821 tatcaaaaac gcatgttact ctatagtggt ttgtctgata tgattattga taccgattat
    11881 cttacaccac aaaaaatagc tactgtaatt ggagagtcac tggttaattt atctaactaa
    11941 gttatcacag tttggtagta tgaactatta ttattgaaag taaatcacct gaaaatatgt
    12001 caagaaataa ttacggtcag ctaaatcatc atgaagagct acgatataac tatcttttaa
    12061 aaaacatcca ttatcttaat gaacgtgaaa agatggaatt tcagtacctt cattataaaa
    12121 aaacagcagt acgaccacag agaaggacag aaagtccacc aactaattct tattatgagg
    12181 aaccttattc tgattcttat tatcaagatg acgattttta ctcagagcct cagctaacct
    12241 cacaaggttt accaatttat caagaagaga gagctccgaa gaagaaaaaa caaagggcca
    12301 gaaaagaaaa acagagggtg aaggttatgg cgcctttccc tccaaaggct ataacccccc
    12361 ctagaaagaa aaaaaaattt aagggtttcc taaagttcat aggtattata ttactaattg
    12421 ttttatcagg tatggttttc atgtttgtta aaggaatgcg tgatgtgaat aatgggaagt
    12481 cacattatag ccctgcaatt attgaagatt ttaagggaaa agatgctgtt gatgggacta
    12541 atatcttaat tttaggtagt gataaacgtg tcagcgagag atctactgat gcgagaacag
    12601 ataccataat ggttgctaat gttggtaaca aggataataa ggttaagatg gtgagtttta
    12661 tgcgagatct cttagtcaat attcctaatt atagtacgga aggttactat gatatgaaat
    12721 taaatgcgtc ttttaatttg ggagaacaag ataatcataa aggagcagag tatgttcgac
    12781 aaacgttgaa aaatcatttt gatattgata taaaatatta tgttatggtt gactttgaga
    12841 catttgcaga tgctattgat acactgtttc ctaatggtgt caagattaat gctaaatttg
    12901 gacttgtcgg tggacaatct gctgattcag ttaaagttcc tgatgattta aggatgaaaa
    12961 atggcgttgt acctagccaa aaaattaagg ttgggattca gtacatggat ggaaggaccc
    13021 ttctaaatta tgctcgtttc agaaaagacg atgatgggga ctttggtcga acacaacgtc
    13081 aacaacaagt tatgagagct attgtctctc aaataaaaga cccacgtcgt ctctttacag
    13141 gttctgctgc tatcggtaaa gcctatgctt taacttcaag taatttatct tatagctttg
    13201 tcctaacaga tggcattcca attttatcag atgctaaaaa tggtattaag caaatgacaa
    13261 ttccaagaga aggagattgg gtagatgatt atgaccagta tggtggtcaa gggttaacaa
    13321 ttgattttgc taagtacaaa aagatcctaa agaaaatggg actgcgttaa catttttaat
    13381 tatgatataa tagagtgatt tattgtcact ctatttttat ctttagtgta taaactaaag
    13441 acttcaaatg atagatataa aaggaaaatt atgttacata aaaatgatat tattgaaact
    13501 gaaattagcg acattagcca tgagggaatg ggtattgcca aagtagatgg ctttgttttc
    13561 tttgttgaaa atgctcttcc aggtgagatt attaagatgc gcgtcttgaa attacgtaag
    13621 cgtataggat atggaaaagt tgaggaatat ttaacgactt ctcctcaccg aaatgaagga
    13681 ttagactata cctatttacg tacagggatt gctgatttgg gacatcttac ttatgaacaa
    13741 cagctgttat ttaaacaaaa acaagtagct gataaccttt ataaaattgc tcatatatct
    13801 gatgttttag tagaacctac tcttggtatg acaatccctt tggcatatcg aaataaagct
    13861 caggtaccag ttcgtcgtgt tgatggacag ctagagacag gttttttcag aaaaaattca
    13921 catactctgg tatccattga agattattta attcaggaaa aagagattga tgctctgatt
    13981 aattttacac gagatttatt aagaaaattt gatgttaaac catatgacga agagcagcaa
    14041 tctggtttaa ttagaaatct cgttgttcgt cgaggacatt atacagggca attaatgttg
    14101 gttttggtga caacacgtcc taaaatattc cgcattgacc aaatgattga aaagttggtg
    14161 tctgcctttc cttctgttgt ttcaattatg caaaatatta atgatcgtaa tagcaacgtt
    14221 atttttggga aagaatttcg tactctatat ggttctgata ctattgagga ccaaatgtta
    14281 ggaaatactt acgctatatc agcccaatct ttttaccaag ttaatactga aatggctgaa
    14341 aaactgtacc aaaaagcaat cgatttttca gatttaaact cagaggatat cgttattgat
    14401 gcctattcag gtataggaac aattggctta tctgttgcta aacaagtaaa acatgtttat
    14461 ggtgttgaag ttgttgaaaa agcagtaagt gatgccaaag aaaatgctac tcgaaatggg
    14521 ataactaaca gtacctatgt tgctgattcg gctgaaaatg caatggcaaa gtggcttaaa
    14581 gaaggtataa aaccaactgt tataatggtt gatccaccac gaaaaggatt aacggagagt
    14641 tttgtatatt ctgctgccca aacaaaagca gataaaatca cttatatttc ttgtaattca
    14701 gcgacaatgg cgcgtgatat caaactattt gaagaattag ggtatcattt agtaaaaatt
    14761 caaccagtag acttattccc aatgacacat catgtggagt gtgtggctct gctcgtaaaa
    14821 gcctagaaac tttttaacga aaggaatatt aaaaagcctt agataatcga ctgacctatt
    14881 accaaaaaat cgaaggggct ctacttgcca ttgcacaaac accaggaaat agtttgccaa
    14941 ctatgcagct gttagatgag ttagttcttc caaaagacat taccatagca gatgtcagtc
    15001 aaagtatcca agaatttgaa atagaaagag atgcccttca agaatttttt gacgatatgc
    15061 tgaagactta tacaacctac caagaaatga aagattatat taaatctcgt gaagcgaaca
    15121 ctcctaagca agagacagaa gaaaaatcac tttgaagact gtagacttaa cataagtaaa
    15181 aaataaccat atatgatatc gctttcattc ttatgttttt tgtgataaac taattaaaga
    15241 aataattttt atactttatt atggaggttc ttatgaagaa agtgttagtg agtagtcttt
    15301 tggttttagg gattacgata acgttacaaa cagtagttga ggctaaggga ccaaaagtag
    15361 cttatacaca agagggaatg actgctcttt cggacacaaa taaagataaa gtcactacta
    15421 tttctattga cgagattcaa aaaagcttag aaggtaagaa gccgattact gttagttttg
    15481 atattgatga tacactgctt ttcagtagtc aatattttca atatggtaaa gaatatgtaa
    15541 ctcctggatc gtttgatttt cttcataaac aaaaattctg ggatcttgtt gcaaaacgag
    15601 gagatcaaga ttccattccc aaagaatatg ctaaaaaatt aattgctatg catcaaaaac
    15661 gaggagataa aattgttttt ataacaggta ggacaagagg gtcaatgtat aaggagggcg
    15721 aggttgataa aacagctaaa gccttagcta aagattttaa attagacaaa ccaattgctg
    15781 taaattatac aggcgataaa cctaaaaagc catacaaata tgataaatca tattatatta
    15841 agaaatatgg ttcagacatt cattatggag atagtgatga cgatattcat gcagctaggg
    15901 aggccggtgc tagaccaatt agaattttaa gagcacctaa ttctacaaat ctacctttac
    15961 cagaagctgg aggctacggt gaagaggttc tcgaaaattc agcttactag tatctttatt
    16021 agttaacata gtattatcta tcaattttta gatttgtaat aagaagtgct aaaaaagtag
    16081 caatcaatga tatgctaata agggaagttg gagtagttta gattgattat taatgactga
    16141 tcttctctcc tctcggtaac aagaagtttt tctaacaaat gcctgtctaa aaaaattgaa
    16201 tcataatttt ttaataactc ttttggcgtg aacaaggaat ttgtaatcag tcatctaatt
    16261 ctataataca atactttggg gttgtttccg tatcattatt aataatatgt atcagaaagg
    16321 agatgttttt tgagagttta tgaaaataaa gaagagttga aaaaagaaat aagtaaaaca
    16381 tttgagaaat acattatgga atttaataat attccagaga atctaaaaga taaaagaatt
    16441 gatgaagttg atagaactcc agcagaaaac ctttcttatc aggttggctg gaccaacttg
    16501 gttcttaaat gggaagaaga tgaaagaaag ggacttcaag taaaaacacc atcggataaa
    16561 tttaaatgga atcaacttgg tgaattatat cagtggttca cagataccta cgctcattta
    16621 tctctgcaag agttgaaagc aaaattaaat gaaaatatta attctatcta tgcaatgatt
    16681 gatttgttga gtgaggaaga attatttgaa gcgcatatga gaaagtgggc tgatgaagcg
    16741 actaaaacag cgacttggga agtgtaaagt ttattcatgt aaatacggtt gcaccttttg
    16801 gaactttcag aactaaaatc agaaaatgga agaagatagt attataaatt atatttttaa
    16861 ctttaaaaaa tttcataaaa atggttacca aagacgatag ttttttctaa tacaaatatt
    16921 aaattctcac gacagatgta atatatcttg ctatttcttc aggctcttca gtaaagtcat
    16981 gatctatcca atttaaaata atactttcaa ttgttgagat ataaatacca agagcgaaag
    17041 aatatggaac ttgatatgct gtttccagtt gtt
//
LOCUS       DATRUK010000043        16758 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.043, whole genome shotgun sequence.
ACCESSION   DATRUK010000043 DATRUK010000000
VERSION     DATRUK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16758
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.043"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(73..1341)
                     /locus_tag="VBE34_001357"
     CDS             complement(73..1341)
                     /locus_tag="VBE34_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEO7264072.1"
                     /translation="MKIDKRHLLNYSILIPYLILSILGLIVIYSTTSATLIQLGANPF
                     RSVINQGVFWAVSLVAIIFIYKLKLNFLKNSKVLTMAVLVEVFLLLIARFFTQEVNGA
                     HGWIVIGPISFQPAEYLKVIIVWYLAFTFARRQKKIEIYDYQALTKGRWLPRSLSDLK
                     DWRFYSLFMIGLVIAQPDLGNGSIIVLTVIIMYCISGIGYRWFSALLGLIVVGSTLFI
                     GTIAVVGVETMAKVPVFGYVAKRFNAFFDPFKDLTDSGHQLANSYYAMSNGGWFGRGL
                     GNSIEKLGYLPEATTDFVFSIVIEELGVIGAGFILALVFFLILRIMHVGIKAKDPFNS
                     MIALGIGAMLLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAIGFVLNIDAN
                     EKKELIMKEAEEQYKPQEKNEKIINLDAFK"
     gene            complement(1447..4242)
                     /gene="ppc"
                     /locus_tag="VBE34_001358"
     CDS             complement(1447..4242)
                     /gene="ppc"
                     /locus_tag="VBE34_001358"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEO7264073.1"
                     /translation="MSHPKLESSSNKEIITEEVGLLKQLLDEATQKLIGSESFDKIEK
                     IVSLSSTDDYTGLKETISALSNEEMVIVSRYFSILPLLINISEDVDLAYEINYKNNLN
                     QDYLGKLSTTIDVVAGHENAKDILEHVNVVPVLTAHPTQVQRKTVLELTSKIHDLLRK
                     YRDVKAGIVNQEKWYADLRRYIGIIMQTDTIREKKLKVKNEITNVMEYYNRSLIKAVT
                     KLTAEYKALAAKKGIHLENPKPLTMGMWIGGDRDGNPFVTAETLRLSAMVQSEVIINH
                     YIEQLNELYRNMSLSISLTEVSPELVTLANQSQDNSVYRENEPYRKAFNFIQDKLVQT
                     LLNLKVGSYPKEKFVSRQESSGIVGRYIKSHIAQVASDIQTEELPAYATAEEFKQDLL
                     LVKQSLVQHGQDSLVDGELACLIQAVDIFGFYLATIDMRQDSSINEACVAELLKSANI
                     VDDYSSLSEEEKCQLLLKELTEDPRTLSSTHTPKSELLQKELAIFQTARELKDQLGED
                     IIKQHIISHTESVSDMFELAIMLKEVGLIDANQARIQIVPLFETIEDLDNSRDIMTQY
                     LHYGLVKKWITTNNNYQEIMLGYSDSNKDGGYLSSGWTLYKAQNELTKIGEENGIKIT
                     FFHGRGGTVGRGGGPSYEAITSQPFGSIKDRIRLTEQGEIIENKYGNQDAAYYNLEML
                     ISASIDRMVTRMITNLNEIDNFRETMDGIVAESNAVYRNLVFDNPYFYDYFFEASPIK
                     EVSSLNIGSRPAARKTITEISGLRAIPWVFSWSQNRIMFPGWYGVGSAFKQFIEQDEA
                     NLAKLQTMYQKWPFFNSLLSNVDMVLSKSNMNIALQYAQLAGSKEVRDVFNIILNEWQ
                     LTKDMILAIEQHDNLLEENPMLHASLDYRLPYFNVLNYVQIELIKRLRSNQLDEDYEK
                     LIHITINGIATGLRNSG"
     gene            4451..6250
                     /gene="pepF"
                     /locus_tag="VBE34_001359"
     CDS             4451..6250
                     /gene="pepF"
                     /locus_tag="VBE34_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO7264074.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKALEDIKVFKKN
                     YEGKLNCVEDFTSALMEIEHIYIQMSHIDTYAFMPQTTDFSNEEFAQISQAGSDFATK
                     ANVLLSFFNTALANADIKILDSLENNPHFKATIRQAKIQKQHLLSPEVEKALTNLNEV
                     LNTPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVTYENYFQNHENAEIREKSFRSFSKG
                     LRKHQNAAAAAYLAKVKSEKLIADMRGYDSVFDYLLSEQEVDRSMFDRQIDLIMDEFG
                     PVAQRFLKHIADVNGIEKMTFADWKLDIDNELNPEVSINDAYDLVMKSVAPLGKEYSQ
                     EVERYQKERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSFFNTHMSTYYVEAPSTFNELLLSDYLENQFDTARQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGAEQLNAIMKEVLTQFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLNLKNNPNGAKEWLAFLKSGGSCTPLETALLIG
                     ADISTDKPLRDTINFLSNTVDQIINYSNLIKQG"
     gene            complement(6309..6698)
                     /locus_tag="VBE34_001360"
     CDS             complement(6309..6698)
                     /locus_tag="VBE34_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEO7264075.1"
                     /translation="MKKYIKLFLLTVFATTLVACGQPSTSNKTTTSSTLEVGKVELVV
                     KEDTNVLSEKVVYHKGDTVLDVLKANYKVKEKDGFITSIDGISQDETKGLYWMFKVNN
                     KLAPKAANQIKVKKNDKIEFYQEVYKK"
     gene            complement(6682..7206)
                     /locus_tag="VBE34_001361"
     CDS             complement(6682..7206)
                     /locus_tag="VBE34_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7264076.1"
                     /translation="MIKNSTLTKLTRVAILSALCVVLRYAFAPLPNIQPITAIFLITV
                     VLFDLKEGVATVTVTMLVSSFLMGFGPWVFLQIISFTLILCLWKFLIYPLTKAVCFGK
                     ITEVVLQTFFAGGLGVVYGVIIDTCFAWLYHMPWWTYVLAGLSFNMAHALSTCLFYPL
                     LLPILRRFRNEKIH"
     gene            7363..8211
                     /locus_tag="VBE34_001362"
     CDS             7363..8211
                     /locus_tag="VBE34_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEO7264077.1"
                     /translation="MRRRIKPIVVAVFFSLFGLLLIIGHLHSTNTLKKELVEAKKTIP
                     SVKASKVPQKSTSSKDKEFVLKPIIDVSGWQLPKEIDYDTLSKNISGVVIRIFGGSKI
                     SKTNNAAYTTGIDKSFKTHIKEFQKRNIPVAVYSYALGSSVKEMKEEAQIFYKNAAPY
                     KPTFYWIDVEEETMSNMNKGVQAFRKELKRLGAKNVGIYIGTYFMTEQGISVKGFDAV
                     WIPTYGSDSGYYEAAPQTELKYDLHQYTSQGYLPGFNQPLDLNQIAVNKDKKKTYEKL
                     FGKVKD"
     gene            complement(8244..8861)
                     /locus_tag="VBE34_001363"
     CDS             complement(8244..8861)
                     /locus_tag="VBE34_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEO7264078.1"
                     /translation="MEILIAGGSGFLGKQIIKAALTKGHKVAYLSRHEGKGDIFKDPR
                     LTYIKGDITEADKIHLEHRNFDILIDCIGAIKPNQLDELNVKATQKAVALCHKNQIPK
                     LVYISANSGYSAYIKSKRKAEQIIKASGLDYLFVRPGLMYGEERPLSIFQAKCIKLFS
                     HLPFLGIVVQKVFPTKVVIVAEAIVTSLRKKPTQKILSIEELNNK"
     gene            9155..9628
                     /locus_tag="VBE34_001364"
     CDS             9155..9628
                     /locus_tag="VBE34_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEO7264079.1"
                     /translation="MSKKNKIKKTLVDQILDKAKIEHDSLQLDALQGDLPNGIQKQDI
                     FKTLALIGDKTGPIIGILPLTEHLSEKKLAKISGNKKVQMIPQKDLQKITGYIHGANN
                     PIGIRQKHNYPIFIDTIALEKQELIVSAGEIGRSIRINSEVLADFVNAKFADIKE"
     gene            9650..10291
                     /locus_tag="VBE34_001365"
     CDS             9650..10291
                     /locus_tag="VBE34_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO7264080.1"
                     /translation="MKFYLVRHGKTQWNLEGRFQGANGDSPLLEEAIEELEELGQYLS
                     SIHFDAVYSSDLGRARDTVNILNDANSCPKEIHYTSQLREWALGTLEGCKIATMQAIY
                     PRQMTAFYQNPLQFKHDMFGAESLYQTTHRVESFLRSLASKNYDKVLIVGHGANLTAS
                     IRSLLGYQYGSLHYKDKLDNASLTIIETHDFKDFNCLTWNDKSYLRQEVKMTH"
     gene            complement(10327..11229)
                     /locus_tag="VBE34_001366"
     CDS             complement(10327..11229)
                     /locus_tag="VBE34_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7264081.1"
                     /translation="MITSIVFDVDDTIYDQQAPYRIAVEKCFPDFDMSAINQAYIRFR
                     HYSDIGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEATGIYFQEIYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVKKLGLYDYVDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPSTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRSLKPGIKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDINDKSNPVLEMGLNNGLMMASERLLESNMS
                     VDKVVILLRLTAKQEKVLRMKYAR"
     gene            11404..12894
                     /gene="lysS"
                     /locus_tag="VBE34_001367"
     CDS             11404..12894
                     /gene="lysS"
                     /locus_tag="VBE34_001367"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEO7264082.1"
                     /translation="MSNQHIEELNDQQIVRREKMAALTEQGIDPFGKRFERTATSGQL
                     NEKYADKSKEDLHDIEETATIAGRLMTKRGKGKVGFAHIQDREGQIQIYVRKDSVGEE
                     NYEIFKKADLGDFLGVEGQVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDIETI
                     YRKRHLDLISNRDSFDRFVTRSKIISEIRRFMDSNGFLEVETPVLHNEAGGASARPFI
                     THHNAQDIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEAYQ
                     AYADYQDIMDLTEGIIQHVTKTVKGDGPINYQGTEIKINEPFKRVHMVDAVKEITDID
                     FWKEMTLEEAQALAQEKNVPLEKHFTTVGHIINAFFEEFVEDTLIQPTFVFGHPVEVS
                     PLAKKNDTDPRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQASAKELGDDEATG
                     VDYDYVEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(12969..13439)
                     /locus_tag="VBE34_001368"
     CDS             complement(12969..13439)
                     /locus_tag="VBE34_001368"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEO7264083.1"
                     /translation="MTIIEGQLVANEMKIGIVVSRFNELITSKLLSGAVDGLLRHGVS
                     EEDIDIVWVPGAFEIPYMARKMALYKDYDAIICLGVVIKGSTDHYDYVCNEVTKGIGH
                     LNSQSDIPHIFGVLTTDNIEQAIERAGTKAGNKGYDCALSAIEMVNLDKKLRER"
     gene            complement(13454..14647)
                     /locus_tag="VBE34_001369"
     CDS             complement(13454..14647)
                     /locus_tag="VBE34_001369"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEO7264084.1"
                     /translation="MAFSPIKKLLQDIKSGEMVVLMDDENRENEGDLICAAEMVTKES
                     INFMAKFGKGLICLPLSNYYAEKLELAQMASHNTDNYETAFTISIDHLSTSTGISAED
                     RALTAKMVANDSSKAKDFRRPGHLFPLLAKEGGVLARNGHTEATVDLCRLAGLKECGL
                     CCEIMAEDGSMMRKDELLAFAQKHDLAIATIKQLQDYRRQEEGGVVREIEIQLPTQFG
                     HFKAYGYSEVVANKEHVALVKGDISSGEDVLCRLHSECLTGDVFHSLRCDCGEQLANA
                     LQQIEAEGRGVLLYMRQEGRGIGLINKLKAYHLQEEGLDTLEANLALGFEGDERDYGV
                     SAQLLKDLGINSINLLTNNPDKIQQLEAEGICVKNRVPLQVAATAYDLNYLKTKKEKM
                     GHLLD"
     gene            complement(14665..15315)
                     /locus_tag="VBE34_001370"
     CDS             complement(14665..15315)
                     /locus_tag="VBE34_001370"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEO7264085.1"
                     /translation="MFTGIIEEMGQVSRIRNGIKSQQLSIDVPKLVPLLRKGDSVAVN
                     GVCLTVLDKSEKAFIADVMPESMMRTSLAALRLHSKVNLELALRSDSRLGGHFVLGHV
                     DGVGKIEKIQKDDIAVRFSIDAPPSIMSYIIEKGSVALDGISLTVVSFTEHSFEVSVV
                     PHTMAQTNLSLKKVGDLLNIEVDVLGKYAEKFLAPTNRTNHTSSVMDWSFLSENGY"
     gene            complement(15296..16405)
                     /gene="ribD"
                     /locus_tag="VBE34_001371"
     CDS             complement(15296..16405)
                     /gene="ribD"
                     /locus_tag="VBE34_001371"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEO7264086.1"
                     /translation="MMKKEDYMALALKEAEKGMGFVAPNPLVGAVIVKDDRIISKGYH
                     KRFGDLHAERQAIKNADEDISGSTLYVTLEPCCHVGKQPPCTEALIKSGIKKVVVGSL
                     DPNPLVSGKGIALLRKEGLNVEVGILREECDALNERFIFHMTYKQPFVYLKYAMTLDG
                     KIATKTGDSKWISNEHSRQSVQKLRQKCSAIMVGINTVLADNPRLTCRIPKGEALVRI
                     VCDSQLKIPLDSYLVKSAKTIPTWIATCSDNLAQQQTLKEMGCRLIKVPRKDGKLDLK
                     VLMTILGQEGIDSLLIEGGSSLHFSALEAGIVNRLIVFIAPKIIGGLKAKTAISGEGL
                     DWLNQAFRVKDIELSRMDSDVVIEGKVEHYVYRNY"
     regulatory      complement(16599..16713)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         5482 a         3250 c         2605 g         5421 t
ORIGIN      
        1 ctctattaat tagagtttaa atttcaaaaa atacaaaaat cgagataaaa atctcgtttt
       61 ttgtttggat gattatttaa aggcatcaag gttgattatt ttttcatttt tttcttgtgg
      121 cttatattgc tcttctgctt ctttcattat gagttccttt ttctcgttag catcaatatt
      181 aagcacgaat ccgatagcta cagaaagtac caagaggctg ttccctcctt gggataagaa
      241 aggaaatgtc actccagttg aggggattaa cccagaaata ccaccaatgt taacaaaaac
      301 ttgcatcaat agcattgctc caataccaag agcgatcatt gagttgaaag gatctttagc
      361 ctttatacct acgtgcataa tacgtaaaat taagaagaaa accaaagcta aaataaaccc
      421 tgcaccaata actcctaatt cctcaataac aattgaaaag acaaagtctg ttgttgcttc
      481 tggtaagtac cctaattttt caatagaatt cccaagtcct cgaccaaacc agccaccgtt
      541 gctcattgca taatatgaat tagccaactg gtgtcctgaa tctgttaaat ctttaaatgg
      601 gtcaaaaaaa gcattaaatc gcttagctac ataaccaaag acagggactt tagccattgt
      661 ctcaactcca acaactgcaa tagttccgat aaataaagtt gaccccacga cgattagacc
      721 aagtaaagct gaaaaccatc gataaccaat accactaata cagtacataa taatgaccgt
      781 tagaacaatt atcgaaccat ttcctaaatc aggttgtgca attaccaaac caatcataaa
      841 taaagaataa aatcgccaat cttttaggtc gcttagtgat ctcggaagcc atctcccttt
      901 tgtcaaagct tgataatcat atatttctat ctttttttgc cttcgagcaa aagtaaacgc
      961 taaataccat acaatgataa cttttaaata ttcagcaggt tgaaaactaa taggaccaat
     1021 aacaatccaa ccatgtgctc cattaacttc ttgtgtgaaa aagcgcgcta ttagtagcaa
     1081 aaatacttct accaatactg ccatagtcaa cactttagaa tttttcaaaa aatttagttt
     1141 cagtttatag ataaagataa ttgcaaccaa actgactgcc caaaagacac cctggttgat
     1201 tactgaacga aaagggttag ctccaagctg aattagagtt gcactagttg tcgaataaat
     1261 tacaattaag cctagaatag acaatatcaa atagggaatg aggatggaat aattcagaag
     1321 atgcctttta tcaattttca tattgtatta tttcctttaa tatatcaaaa aattaccatt
     1381 atgcattata tcatctttcg aagtaaagta aaagggagaa tagcaatgat cctccctcta
     1441 tcaatattaa cctgaatttc gtaaacctgt tgcaatacca ttaatagtaa tatgaatcaa
     1501 cttctcatag tcttcatcta gttggttact acgaagacgt ttaatcaatt caatttgaac
     1561 ataattcaaa acattgaaat atggcaaacg ataatctaaa cttgcatgta acattgggtt
     1621 ttcttctaat aaattatcat gctgttcaat agctaaaatc atatctttag ttaactgcca
     1681 ttcattaagg atgatattaa aaacatctcg aacttcttta cttccagcta actgagcata
     1741 ctgaagcgca atattcatat ttgattttga taaaaccata tctacattag ataaaagaga
     1801 attaaagaat ggccattttt gatacatggt ttgtaatttt gctaagtttg cctcatcttg
     1861 ttcaataaat tgcttgaagg ctgacccaac accataccaa cccggaaaca taatacgatt
     1921 ttgtgaccat gaaaataccc atggaattgc acgtaaacct gaaatttctg taattgtttt
     1981 acgtgctgct ggccgagaac caatatttaa acttgatacc tctttaattg gacttgcttc
     2041 aaagaaatag tcatagaaat aaggattatc aaaaactaaa tttctataaa cggcattact
     2101 ttcagccaca ataccatcca ttgtctcacg gaaattatca atttcattaa ggttagtaat
     2161 catacgtgtt accatgcggt ctatggaagc tgaaataagc atttcaaggt tataataggc
     2221 tgcatcttga ttaccatatt tattctcaat gatttcacct tgctctgtta ggcgaattcg
     2281 atctttgatt gaaccaaaag gttgtgatgt gatagcttca taagatggac caccaccacg
     2341 accaacagtt ccaccacgac cgtggaaaaa tgtgatttta ataccgtttt cttcacctat
     2401 ttttgttagc tcattttgag ctttatatag agtccaacca gatgataaat agccaccatc
     2461 tttattacta tctgaataac ctagcattat ttcttgataa ttgttgttag ttgtaatcca
     2521 tttttttact aatccataat gtagatactg ggtcataata tcacgtgaat tatccaaatc
     2581 ttcgatggtt tcaaataacg gtacaatctg aatgcgagct tgattggcat caattagtcc
     2641 aacttctttt aacatgatag ctaactcaaa catatccgaa acgctctctg tgtgagaaat
     2701 gatatgctgt tttataatgt cttcacccaa ttgatctttt aactcacgtg ctgtttggaa
     2761 aatagccaat tctttttgaa gtaactcgga tttaggagta tgcgtagaag ataatgttct
     2821 aggatcttct gttaattctt ttaagagaag ttgacatttt tcttcttctg aaagagaact
     2881 gtaatcatct acaatattgg cagacttcaa taattctgct acacaagctt cattaatact
     2941 agaatcttga cgcatatcaa tagtagctaa ataaaatcca aagatatcta ctgcttgaat
     3001 aagacaagct aattcgccat cgacaagaga atcttgtcca tgttgaacta aagattgctt
     3061 cacaagtaac aaatcttgtt taaactcctc agcagtagcg tatgctggta gttcttctgt
     3121 ctgtatgtca gaagctactt gagcgatatg tgattttata taccttccta caataccaga
     3181 agattcttgg cgactaacaa atttctcttt tgggtaagaa ccaactttta aatttagtag
     3241 ggtttgtact agtttatctt ggataaaatt gaaagcttta cgataaggtt cattttccct
     3301 atatactgaa ttatcttgtg actggtttgc caacgtaaca agttcgggag agacttcagt
     3361 tagacttatt gaaagtgaca tattacgata aagttcattt agctgctcaa tataatggtt
     3421 aataatcact tcactttgta ccatagctga taatcttagt gtctcagcag ttacaaaggg
     3481 attgccatct ctatcaccac caatccacat ccccatagtc agaggcttag gattctctaa
     3541 gtgaatacct tttttagctg ccagtgcctt atattctgct gttaatttgg taactgcttt
     3601 aataagagaa cggttgtaat attccattac attggttatc tcatttttga ctttcaattt
     3661 tttctcgcga atagtgtctg tttgcatgat aatgccgatg taacgacgca agtcagcata
     3721 ccatttttct tgattcacga ttcctgcttt gacatctctg tatttacgta ataaatcatg
     3781 aatcttagac gttaattcga gcaccgtttt acgctgcacc tgagtaggat gagctgttag
     3841 cacaggcaca acattgacgt gttcaagaat atctttagca ttttcatggc cagctacaac
     3901 atcaatagtc gttgataact ttcctaggta atcttggtta agattatttt tataattaat
     3961 ttcatatgct aaatcaacgt cttctgaaat attaattaag agcggcaaaa tagagaagta
     4021 acgagaaaca ataaccatct cctcgttgct taaagccgaa atagtttcct tcaaaccagt
     4081 ataatcgtca gttgatgata atgaaactat tttttcaatc ttgtcaaatg attcactacc
     4141 aatcaatttt tgcgttgctt catccaaaag ctgcttcagt aaaccgacct cttcggtaat
     4201 gatttcttta ttgctactac tttctaattt cgggtgggac aaaccatcac ttcctctctt
     4261 aaatctcact actatgatac cattttaaag taaaaagtaa accattctag gatttttaga
     4321 tagaattttc agatttttac ttatttatga cagtaatcag tttttcttgt tcgacttttt
     4381 tgatatactg aggataattg agattaactg attaattgtt aattaacaac tattattaaa
     4441 ggagtatcgc atggaactta aaaaacgctc tgaattccct gaaaatgagc tttgggatct
     4501 gactgctctc tacaaagatc gtcaagattt tttactggct attgaaaaag ctcttgagga
     4561 tatcaaggtt ttcaaaaaaa attacgaggg aaaacttaat tgtgttgagg attttactag
     4621 tgcccttatg gaaatcgagc atatttatat ccagatgagt cacattgata cctatgcttt
     4681 tatgcctcaa actactgact ttagcaacga agagtttgca caaatttctc aagcaggatc
     4741 agacttcgca actaaagcta atgtactact ttctttcttt aatactgccc tagcaaatgc
     4801 agatataaag attttagatt ctttagaaaa taaccctcat ttcaaagcaa ctattcgcca
     4861 agctaaaatt caaaagcaac acttattaag tccagaagtc gaaaaagctt tgacaaactt
     4921 aaatgaagtc ctaaatacac catatgatat ttatactaaa atgcgtgctg gcgattttga
     4981 tatggaagat tttgaagttg acggaaaaac ctacaaaaat agttttgtaa cttatgaaaa
     5041 ttactttcaa aatcacgaaa atgcagagat acgtgaaaaa tccttccgtt cattctcgaa
     5101 aggtctacgt aagcaccaaa atgctgcagc tgcggcttat cttgcaaaag tcaaatctga
     5161 aaagttaatt gctgatatgc gtggttacga tagtgttttt gattatcttc tttccgaaca
     5221 agaagttgac cgttcaatgt ttgacagaca aattgatctt atcatggatg aatttggtcc
     5281 agtagctcaa cgattcctca aacatatcgc cgatgttaat ggtattgaaa agatgacttt
     5341 tgcagattgg aaattagaca ttgataacga acttaatccc gaagtaagta tcaatgatgc
     5401 ttatgactta gtaatgaaat cagttgctcc tttaggaaaa gaatattccc aagaggttga
     5461 gcgctatcaa aaagagcgtt gggttgattt tgcagctaac gctaataaag attctggagg
     5521 atatgctgcg gatccttaca aagttcatcc ctatgtactc atgagctgga cggggcgtat
     5581 gagcgatgtc tacacactta ttcatgagat tggacattct ggtcaattta tcttctcaga
     5641 taatcaccaa agttttttca atactcacat gtccacttac tatgttgaag ctccatctac
     5701 ttttaacgaa cttttactta gtgactactt agaaaaccaa tttgatacag cacgtcaaaa
     5761 acgctttgct cttgcacatc gcttaactga tacttacttc cacaatttca ttactcactt
     5821 acttgaagct gctttccaac gtaaagttta cactctaatt gaggagggag aaacttttgg
     5881 agcagaacaa ttaaatgcta ttatgaagga agtattaaca caattctggg gagatgctat
     5941 tgaaatagat gacgatgctg ctttgacatg gatgagacaa gctcattact acatggggct
     6001 ctatagctat acttactctg ctggtttagt aatctcgaca gccggctatc tcaatctcaa
     6061 aaataatcca aacggagcaa aagagtggtt agcattcttg aaatctggtg ggagctgtac
     6121 ccctttagaa acagccctat taatcggtgc tgatatttct actgataagc ctctacgcga
     6181 tactattaat ttcttatcta acactgtaga tcaaattatt aattatagta atcttattaa
     6241 acaaggctaa actacagtct ttcaaaaaca taaaaacaag ttggagatat ctccaacttg
     6301 tttttatctt attttttgta aacttcctga taaaattcaa ttttgtcgtt ctttttaact
     6361 ttaatttggt ttgctgcctt aggagctaat ttattattga ccttgaacat ccaataaagc
     6421 cctttggttt catcttggct gatgccatca attgaagtaa tgaaaccatc tttttcttta
     6481 actttatagt tagctttaag cacatccaaa acagtgtcac ctttatggta aacaaccttt
     6541 tcacttaaca cgttcgtatc ttctttaaca accaattcaa ccttaccaac ttctaacgtt
     6601 gagctagttg ttgtcttatt cgatgtagat ggttgcccgc aagctactaa agtcgtagcg
     6661 aatactgtta gcaagaataa tttaatgtat tttttcattc ctaaatctcc ttaaaatcgg
     6721 taacaaaaga ggataaaata aacaagttga taaagcgtgt gccatattaa aactaagtcc
     6781 tgctaataca taagtccacc aaggcatatg atataaccaa gcaaaacagg tatcaataat
     6841 gacgccataa acaaccccta accctcctgc aaagaaagtt tgaagtacta cctccgttat
     6901 tttaccaaaa catacagctt ttgtcagcgg atatatcaga aatttccaca agcataaaat
     6961 taaagtgaag gaaatgattt gcaaaaagac ccatggccca aagcccatta aaaaagaaga
     7021 aactagcatt gttacagtta cagtagctac accttctttt aaatcgaata agacaactgt
     7081 aatcaaaaaa atggcagtaa ttggctgaat gttaggcaga ggcgcaaaag catatctcaa
     7141 cacaacacac aaagcagata atatagctac tctagtcaat ttagttaaag ttgaattctt
     7201 tatcatgtct ttaattatac ctcaaaacta tgactaccgc ttaaaagtct tttttattac
     7261 aatttcattt ctaatgttac ataaacgacc tcttttttta aaagaacgta aataagcctg
     7321 taaactttga ttttttaaac cttttaagtt acaatatcaa taatgagaag aagaattaaa
     7381 cctattgttg ttgcggtttt tttcagttta tttggacttt tattaattat tggtcattta
     7441 cattcgacaa atactttgaa aaaagaatta gttgaagcta aaaagacaat tccatccgta
     7501 aaagcttcaa aagtaccgca aaaatcaaca tcatcgaaag ataaagagtt tgttcttaaa
     7561 ccgattatcg atgtctctgg ttggcaactt cctaaggaga ttgattacga tacgctttca
     7621 aaaaatattt caggtgttgt tattcgtatc tttggtggat caaagatatc taagactaat
     7681 aacgctgctt atacaactgg aatcgataaa tcgtttaaga cccatatcaa agaatttcaa
     7741 aagcgaaata tcccagtagc tgtctacagt tatgcacttg gttcaagtgt taaagaaatg
     7801 aaagaagagg ctcagatatt ttataagaat gcagctcctt acaaaccaac tttttattgg
     7861 attgacgtag aagaggagac aatgtctaac atgaataaag gtgtccaagc attccgaaaa
     7921 gagttaaaaa gacttggtgc taaaaatgtt ggtatctaca tcggtactta ctttatgact
     7981 gagcaaggta tctctgtaaa aggatttgac gctgtttgga ttccaactta tggtagcgat
     8041 tctggatact atgaagcagc tccacaaact gaacttaaat acgatttaca ccaatacacc
     8101 tctcaaggtt atctaccagg attcaatcaa ccgcttgatt taaatcaaat tgcagttaat
     8161 aaagacaaga agaaaactta tgagaaactt tttggaaaag taaaagatta aaagctactg
     8221 ttaacagtag cttttttgat aaactattta ttatttaatt cttcaataga aaggattttt
     8281 tgagttggtt ttttcctaag cgaagtaacg attgcttctg ccactatcac aaccttagtt
     8341 ggaaagactt tttgtacaac aatacctaag aaaggcaaat gactaaataa ttttatacac
     8401 ttggcttgga aaatcgagag aggtcgctct tcaccataca tcaaacctgg tcttacaaaa
     8461 agataatcca gaccgcttgc tttgattatc tgctctgcct tccttttact tttaatgtaa
     8521 gctgaatagc cgctattggc tgaaatgtaa actaactttg gtatttgatt tttgtgacag
     8581 agtgctactg ctttttgggt tgctttaacg ttaagctcat ctagttgatt gggcttaatc
     8641 gctccaatac agtcaattaa tatatcaaaa tttctatgtt ctaaatgaat cttatcagct
     8701 tctgtaatat ctcccttaat gtaggttaat ctaggatcct taaatatatc acctttacct
     8761 tcatgccttg ataagtaagc caccttatgc ccttttgtaa gcgctgcttt tattatctgc
     8821 ttccctagaa aaccactacc acctgcaatc agtatttcca tagttccctc tccttcattt
     8881 tgtaaattta gtatagaaat ttttatatgc cttgtcaaag taatcgttta atacctgacc
     8941 agaatatgat ttcaagtcat tacattagga cacttcatat ttagctattt ataaaacttc
     9001 taatttaaca ttccaaatat actgttgcga tatttattat cattattccc tgaaatggta
     9061 ttcaagctta tttctaagac tcagtagaaa tttactataa tcatctttaa atgttataat
     9121 aaatatgaaa caaacttatt agttaagggt cattatgagt aaaaagaata aaatcaaaaa
     9181 aactttagtt gatcaaattt tagataaagc aaaaatcgaa catgatagtc tacaattgga
     9241 tgctttacag ggagatttac ccaacggtat tcaaaaacaa gatatcttta agacattagc
     9301 gctaatcggt gataaaactg gtcccataat aggtatctta cctctcaccg agcatttatc
     9361 cgaaaaaaag ttagctaaaa tttcaggaaa caaaaaggta caaatgatac ctcaaaaaga
     9421 tctccagaaa ataacaggtt atattcacgg agcaaataat ccaattggaa ttcgccaaaa
     9481 acataattac cctattttta tcgatactat cgctttagaa aaacaagaac tcattgtctc
     9541 tgcaggagaa attggacgtt ctattcgtat taatagtgag gttcttgcag atttcgtaaa
     9601 tgctaaattt gccgatataa aggaataata attaggattg gagaaaaata tgaaatttta
     9661 tcttgttaga cacggaaaaa cacaatggaa tttagaaggg cgtttccaag gtgctaacgg
     9721 agactcacct ctccttgaag aagctattga agaattagaa gaacttggac aatacctatc
     9781 atctattcat tttgatgctg tttattctag cgacttaggt cgtgctagag atactgttaa
     9841 tatcctcaat gatgctaatt catgcccaaa agaaatccac tatacttctc agcttcgtga
     9901 atgggcattg ggaactttag agggttgtaa aattgcaact atgcaagcta tctatcctcg
     9961 tcaaatgacc gcattttatc aaaatccctt acaatttaaa catgacatgt tcggtgcaga
    10021 atctctttat cagacaacgc atcgtgtaga gagtttctta cgttctttag cttcaaaaaa
    10081 ttacgacaag gtccttattg taggacatgg agcaaatcta actgcgtcta ttcgttcctt
    10141 actgggttat caatatggta gtttacacta caaagataaa cttgataatg ccagcctcac
    10201 aatcatagaa actcatgatt ttaaagactt caactgtctg acttggaacg ataaatctta
    10261 tttaaggcaa gaggtcaaaa tgacacacta aaataatcca ctagattcat ctagtggatt
    10321 atttaattat ctagcatact tcatgcgtaa tactttttcc tgttttgcag ttaggcgcag
    10381 taaaataaca actttgtcaa cgctcatatt actctcaagc agacgctctg atgccatcat
    10441 taaaccatta ttaagtccca tttcaaggac tggattactt ttatcgttga tatcaaaaat
    10501 aaatttatta tcaggtaagt caaacaacac tttaacagca ccgaataatt gctcaaagtt
    10561 atcaatagca acatcataaa ctggtttaat tcccggtttt aaagaacgtc ctctatggtt
    10621 gaaccacata gaatgccaac caccattaaa tgcacccata atatcattat catatgaatc
    10681 acccacataa agtgtagttg aagggttcat atcaaattgc tctgctgcta aattaaaaat
    10741 ctccttttca ggcttttgaa aaccagttgc ttgactaact ataacacgtt ttggatcaac
    10801 atagtcataa agtcccaatt ttttaacttt tttcaattga tgttccgtag ggccatttgt
    10861 aataattccc attggtacat tttttgattt caaaaagtca agtgtcatac gcatctcatc
    10921 aagcattgta atattttcaa gttcatgttc gtatatttct tgaaaataaa taccagtagc
    10981 ttcatcaatt tcacgataac caaactctaa aagcgtttct ttgcatcgcc agaaacgaaa
    11041 atattctgtt gtccattcac cagccattac tcgtggaaag ccaatatctg aataatgacg
    11101 aaagcgaata taagcttggt taatagcgct catatcaaaa tcaggaaagc atttctccac
    11161 agcaatacgg taaggtgctt gttgatcgta aattgtatca tcaacatcaa aaacaattga
    11221 agtaatcatg attatccttt tctttctgaa ttttcaattg ttaattatac ctttttctac
    11281 ccttattttc aaatggtgaa aagtctatta aaaatgagaa taacaccata aactcataaa
    11341 ataaattatg aattatcagt gtttttgtgg tacaatagga tgaatactaa cttggaggaa
    11401 attatgtcta accaacatat tgaagaatta aatgaccaac agattgttcg tcgtgaaaaa
    11461 atggctgctt tgactgaaca aggcattgat ccttttggta aacgatttga acgtacagca
    11521 acatctggtc aattaaatga aaaatacgct gacaaatcaa aagaagactt gcacgacatt
    11581 gaagaaactg ctacaatcgc tggtcgttta atgactaagc gcggtaaggg gaaagttggc
    11641 tttgcgcata tccaagatcg cgaaggtcaa attcaaattt atgtccgtaa ggattctgtt
    11701 ggcgaagaaa actacgaaat ctttaaaaaa gctgatttag gtgacttcct tggtgttgaa
    11761 gggcaagtaa tgcgtacaga tatgggagaa ctttctatca aggctactca tattactcac
    11821 ctttcaaaag cactccgtcc acttcctgaa aaattccacg gcctaactga tattgaaaca
    11881 atctatcgta aacgtcatct tgatttaatc tcaaatcgtg acagctttga ccgttttgta
    11941 acacgctcaa aaatcatttc agaaattcgt cgctttatgg atagcaatgg tttcttagag
    12001 gttgaaacac ctgttcttca taatgaagct ggtggtgctt ctgctcgccc atttatcaca
    12061 catcacaatg cacaagacat tgacatggtt cttcgtatcg caactgaatt gcatctaaaa
    12121 cgtcttattg taggtggtat ggagcgtgtt tatgaaatcg gacgtatttt ccgtaatgaa
    12181 gggatggatg caacccacaa tccagagttc acatctattg aagcctacca agcctatgct
    12241 gactaccagg atattatgga cttgacagaa ggtattatcc agcatgttac aaaaactgta
    12301 aaaggtgatg gaccaatcaa ttatcaaggc acagaaatta aaattaatga accattcaaa
    12361 cgtgttcata tggttgatgc ggttaaagaa atcactgata ttgacttctg gaaagaaatg
    12421 actcttgaag aagcacaagc tttggcacaa gaaaaaaatg ttcctcttga aaaacatttc
    12481 acaacagttg gtcacatcat taacgccttc ttcgaagaat tcgttgaaga cactttaatt
    12541 caaccaactt ttgtctttgg tcatccagtc gaagtgtctc cacttgctaa gaaaaatgat
    12601 acagaccctc gttttacaga ccgtttcgaa ctctttatca tgactaaaga atatgctaat
    12661 gcttttacag aattaaatga cccaattgat cagctatctc gctttgaagc acaagcatct
    12721 gctaaagaac ttggcgatga tgaagcaact ggagttgatt acgattatgt tgaagcactt
    12781 gaatatggta tgccacctac tggtggacta ggtattggta tcgaccgtct ctgtatgctg
    12841 ttaactgaca caacaactat ccgagacgtc ctacttttcc ctacaatgaa ataagaatat
    12901 attaaaaccc acccttattt aaggtgggtt ttaatattac ctctactgaa ctttgttagg
    12961 tttctgattt atcgctcacg caatttttta tcaaggttta ccatttcaat agccgaaaga
    13021 gcacaatcat aacctttatt tcctgctttt gttcctgccc tctcaatagc ttgttcgata
    13081 ttatccgttg ttagaacacc aaagatatga gggatatctg actggctatt taaatgacca
    13141 ataccttttg tcacctcatt acaaacataa tcataatgat cagtgctgcc tttaatgact
    13201 accccaagac agataatagc atcataatct ttatataatg ccatttttct agccatatag
    13261 gggatttcaa aagcaccggg tacccataca atatctatat cttcttctga aacaccatgt
    13321 ctcaacagtc catctactgc tcctgataat aatttagatg taatcaattc attgaaacgt
    13381 gagactacga ttcctatttt catttcgttt gccactaatt gtccttcaat aattgtcatt
    13441 ttttcttcct cctttaatct aaaagatgtc ccattttttc ttttttagtt tttaaataat
    13501 taaggtcata agctgttgct gccacctgta atggcactct gtttttgacg caaattcctt
    13561 ctgcttcaag ttgttggatt ttatctggat tattggttag taagtttata gaatttatac
    13621 ctaaatcttt taaaagttga gcactaactc catagtctct ctcatctcct tcaaaaccta
    13681 aagccaaatt agcttctaaa gtatctaagc cttcttcttg caaatgataa gcttttaact
    13741 tattaatcaa accaattcct cgaccttcct gtctcatata aagaaggacg ccacgacctt
    13801 cagcctctat ttgttggagt gcattagcca gttgttcccc acaatcacat ctcaaggaat
    13861 gaaagacatc acctgtcaaa cattctgagt gtaaacgaca taaaacatct tcaccactac
    13921 taatatcccc tttaacaagg gcaacatgtt ccttatttgc cacaacttca ctatatccat
    13981 aagccttaaa atggccgaat tgcgttggta gctgaatctc aatttctcta accacccctc
    14041 cctcttcttg acgtctatag tcttgcaatt gttttatggt agcgatagct aaatcgtgct
    14101 tttgagcaaa tgctaataac tcatcctttc tcatcatact accatcttca gccattattt
    14161 cacaacaaag tccacattcc tttaatccag ctaaacgaca tagatcaacg gttgcttcag
    14221 tatgtccatt tctagctaaa acacctcctt ccttagctaa caagggaaat aagtgcccag
    14281 gacgtctaaa gtctttagct ttgctactgt cgttagcaac catcttagct gtcaaagctc
    14341 tatcttccgc agaaatccca gtactagttg atagatgatc aatggatatg gtaaaagctg
    14401 tttcataatt atcagtatta tggctagcca tttgagctaa ttctaacttt tcagcatagt
    14461 aattagacag aggtaagcaa attaatcctt tcccaaattt agccataaaa ttaatcgact
    14521 cttttgtcac catttcagcc gcacaaataa ggtccccttc attctctcta ttttcgtcat
    14581 ccatcaaaac taccatttcc cctgatttga tgtcttgtaa aagttttttt attggactaa
    14641 aggccattcg ctttctcctc tctcttagta gccattttct gataaaaaag accaatccat
    14701 tactgatgaa gtgtgattag ttctattagt cggtgctaaa aatttctcag catatttgcc
    14761 taaaacatcc acttcgatat ttaataaatc cccaaccttt tttaaggata aattagtttg
    14821 agccatcgtg tggggaacga ccgaaacttc aaaagaatgc tctgtaaatg ataccaccgt
    14881 taggctaatc ccatctagag ccaccgaacc tttctctata atataagaca taatactggg
    14941 cggagcatca attgaaaatc tgacagcaat atcatccttt tggatttttt ctatttttcc
    15001 aaccccatcc acatgtccaa gaacaaaatg cccccctaac cttgagtcac ttctgagtgc
    15061 taattctaag ttaactttag aatgaagtcg taaagcagct aagcttgttc gcatcatact
    15121 ttctggcatg acgtctgcta taaaagcttt ttcacttttg tcaaggactg tcaaacaaac
    15181 accattaaca gctacgctat ccccttttct taataaaggg actagtttgg gaacatcaat
    15241 acttaattgt tgggatttta tgccattccg aatacgactg acttgaccca tttcctcaat
    15301 aattcctgta aacataatgt tctacctttc cttcaataac aacgtcagaa tccatacgac
    15361 taagttcaat atcttttact ctgaaagctt ggttaagcca atccaagcct tctccagaaa
    15421 tagctgtttt tgctttcaga cctcctatta ttttgggtgc aataaaaaca attagtctgt
    15481 taacaatacc agcttctaaa gctgaaaaat gtaaactact tcctccttca ataagaaggc
    15541 tatcaatccc ttcttgtcct aaaatagtca ttaatacttt caaatccagc tttccatctt
    15601 ttcttgggac cttaatcaaa cggcaaccca tttcttttaa tgtttgttgc tgagcaagat
    15661 tatctgaaca tgttgcaatc catgttggta ttgttttagc gctcttaact aggtaagagt
    15721 ctaaaggtat cttcaactga ctatcacata cgatcctcac aagcgcttca cctttaggaa
    15781 tacggcaagt taaacgtgga ttatcagcta atacagtatt gataccgacc ataatagcac
    15841 tacatttttg cctcaatttc tgaacagatt gtcttgagtg ctcattggat atccacttag
    15901 aatcgcccgt ctttgtagct atcttcccat ctaaagtcat agcatatttc aaataaacaa
    15961 aaggctgctt ataggtcatg tgaaaaataa atctttcatt cagagcatcg cattcttctc
    16021 ttaggatacc aacttcaaca tttaaacctt cttttcttaa caaggcaatc ccttttcctg
    16081 aaactaaagg atttggatct aaacttccaa ctactacttt tttgatacca gatttaataa
    16141 gagcttcagt acaaggtggt tgcttaccca catgacagca tggttctaaa gtcacgtaca
    16201 gagtacttcc agagatatcc tcatcagcat ttttaatagc ctgtctttcc gcatgtaaat
    16261 ctccaaatcg tttatgataa cctttggaaa ttatacgatc atctttaaca ataacagcac
    16321 ctactaatgg gttgggagca acgaacccca tacctttctc agcctctttc aaggctaatg
    16381 ccatgtagtc ttcttttttc atcatttcct ttttccttct aaaataaaaa cccttagctt
    16441 ctaaaataga aaactaaggg gagggatgag aagtcgttga ttatactaat tagcattata
    16501 ttttaggtag cacaaaaaaa ctaaagactc cctatagaaa actataggga gtctctcatt
    16561 aatccagcac aaataaactg caagtagcag acaatcactg aattcttctc tcatccagac
    16621 tttactgtcg gtactggaat cacaccagtt catgctcgaa agctcgcgga ctataccgcc
    16681 ggttaggatt ttcacctgac cccgaagaaa ttctatttga cagttatttt actaaactca
    16741 tttttatttg tcaataaa
//
LOCUS       DATRUK010000044        16448 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.044, whole genome shotgun sequence.
ACCESSION   DATRUK010000044 DATRUK010000000
VERSION     DATRUK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16448
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.044"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(7..666)
                     /locus_tag="VBE34_001372"
     CDS             complement(7..666)
                     /locus_tag="VBE34_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO7264087.1"
                     /translation="MPVKDFMTKKLVYVSPDTTVAEAADLLREHHLRRLPVVENDQLV
                     GLVTEGTMAEAQPSKATSLSIYEMNYLLNKTKIRDIMIKDIVTVSQYASLEDAIYLMM
                     SRKIGVLPVVDNGQLYGIVTDRDVFKAFLEIAGYGQEGYRLVILADEGIGVLSKVLNR
                     LSSANLSVKRLVIIERKAGKKAVEIQLEGYADKDVLKQELVFDDVIVETLEKTMKKPL
                     S"
     gene            complement(685..1395)
                     /locus_tag="VBE34_001373"
     CDS             complement(685..1395)
                     /locus_tag="VBE34_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7264088.1"
                     /translation="MTMLKVENLSIHYGVIQAVNDVSFEVNQGEVVTLIGANGAGKTS
                     ILRTISGLVRPSQGSISFMGKPIHKLAARKIVGNGLAQVPEGRHVFSSLSVMENLEMG
                     AFLQKDREQNQKMLKKVFDRFPRLEERKNQDAATLSGGEQQMLAMGRALMSRPKLLLL
                     DEQSMGLAPIFIQEIFNIIEDIKKQGTTVLLVEQNANKALTIADKAYVLETGKVVLSG
                     TGKELLVSDQVRKAYLGG"
     gene            complement(1395..2159)
                     /locus_tag="VBE34_001374"
     CDS             complement(1395..2159)
                     /locus_tag="VBE34_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7264089.1"
                     /translation="MALLEVKNLSKHFGGLTAVGDVSMKLHKGELIGLIGPNGAGKTT
                     LFNLLTGVYLPSKGTISLDGKILNGRKPAKIASLGLGRTFQNIRLFKNMTVLDNVLVG
                     LSNHHLSHPIASFLRLPKYYHSEKALRKKALELLEIFGLKAYQDALAKNLPYGKQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTQLISQIKSDFDITIMLIEHDMNLVMQV
                     TERIYVLEYGRLIAHGTPEEIKNNKRVIEAYLGGEL"
     gene            complement(2160..3113)
                     /locus_tag="VBE34_001375"
     CDS             complement(2160..3113)
                     /locus_tag="VBE34_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO7264090.1"
                     /translation="MKENMKSILSWLAIVVLGYLLISVLISMGIFNLYHIQIIETIGI
                     NVILAVGLNLIVGCSGQFSLGHAGFMAIGAYAVAIIGVKMPTYVGFLIAILVGTLVAG
                     GIALGVGIPTLRLKGDYLAIATLGVAEIIRILLVNGGDITNGAAGIMGIPPFTTWSLV
                     YGVAVVSLILAMNFLRSPLGRNTIAIREDEIAAESMGVDTTKVKVIVFVFGAILASIA
                     GSLQAGYVGTVMPKDFSFMMSVNVLIIVVLGGLGSMTGTVLAAILLGLLNMLLQDYAS
                     VRMIIYALALILIMIFRPSGLLGTKELTLSHLFRKTSKGGN"
     gene            complement(3116..3985)
                     /locus_tag="VBE34_001376"
     CDS             complement(3116..3985)
                     /locus_tag="VBE34_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO7264091.1"
                     /translation="MLQQLVNGLILGSIYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FMGYYLINHLHLNFFLALLIAMLGSAFLGVVIEYLAYRPLRKSTRIAALITAIGVSFL
                     LEYGMVYLVGADTRAFPQAIHTVKYNLGPITITNVQLIILGIALSLMLTLQFIVQKTK
                     MGKAMRALSVDSDAAQLMGINVNRTISFTFALGSALAGAGGVLIGLYYNSVQPLMGVT
                     PGLKAFVAAVLGGIGIIPGAAIGGFVIGILETLATALGVSDFRDGIVYAILILIFLIR
                     PAGILGKNIKEKV"
     gene            complement(4091..5257)
                     /locus_tag="VBE34_001377"
     CDS             complement(4091..5257)
                     /locus_tag="VBE34_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7264092.1"
                     /translation="MEKRLSLGALVLASTVLLAACGNVGGGASSTGTKIGKDIKVGYN
                     WELSGNVSSYGNSMKNGADLAVKEINAAGGVGGKKLKVLSQDNKSENAEAATVATNLV
                     TKGANVIIGPATSGAAASSTPKVNAAAVPMIAPAATQDNLVYGSDGKTLNQYFFRATF
                     VDNYQGKLLSQYATDNLKAKKVVLFYDNSSDYSKGVAKSFKESYSGKIVDSMTFSAGD
                     TDFQASLTKLKGKEYDAIVMPGYYTETGLIVKQARDLGISKPVLGPDGFDSPKFVQSA
                     TPVGASNVYYLTGFTTQGSTKAKAFHDHYVKAYGEEPSMFSALSYDAVYMAAKSAKGA
                     KTSIDIKKALAKLKDFKGVTGKMSIDKNHNVVKSAYVVKLEDGKTSSVNIISAK"
     gene            complement(5412..5657)
                     /locus_tag="VBE34_001378"
     CDS             complement(5412..5657)
                     /locus_tag="VBE34_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2129 domain-containing protein"
                     /protein_id="HEO7264093.1"
                     /translation="MNKQERLGMSVYLYYNRDARKLYKYGDVHYHSRRLRYLVIYVNK
                     EDIVSVSKEIKHLKFVKDVRLSAIDDIDQDFVGNLYR"
     gene            complement(5757..6347)
                     /locus_tag="VBE34_001379"
     CDS             complement(5757..6347)
                     /locus_tag="VBE34_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEO7264094.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGQVEDNMANSIIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKSDVQTIVMGMAASMGTIIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQSDMAIAAEHLLKTRHTLEKILADNSGQ
                     SIEKVHDDAERDRWMSAQETLDYGFIDAIMENNNLQ"
     gene            complement(6472..7101)
                     /gene="upp"
                     /locus_tag="VBE34_001380"
     CDS             complement(6472..7101)
                     /gene="upp"
                     /locus_tag="VBE34_001380"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEO7264095.1"
                     /translation="MGKFQVISHPLIQHKLSILRRTTTSTKDFRELVDEIAMLMGYEV
                     SRDLPLEDVEIQTPVATTVQKQLAGKKLAIVPILRAGIGMVDGFLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGAASIKFVCL
                     VAAPEGVAALQEAHPDVDIYTAALDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(7211..8380)
                     /locus_tag="VBE34_001381"
     CDS             complement(7211..8380)
                     /locus_tag="VBE34_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7264096.1"
                     /translation="MITYDFTSLPERFSSNAIKWKAVQKDQEILPLWIADMDFPIFPE
                     MSEAIEDFSHQMVFGYDSPKDSLYQAISNWEVQEHGYQFDKKSLLLIDGVVPAISVAI
                     QAFTKEGDAVLINTPVYPPFARTIKYNNRHLVSNSLLNNNQYFEIDFKQLEKDIIENN
                     VKLYIFCSPHNPGGRVWTKGEIQKIGDICKRYNVILVSDEIHQDLVLFDNVHHSFNTV
                     DSSFKELSVILSSATKTFNIAGTKNSFAIIENEKLRSDFKKRQIANNQQEISSLGLLA
                     TEVAFTKGKQWLKALKMELEGSIEYLYEQLTQKTHIKVMKPEGTYLVWLDFSAYNLTH
                     LEIQEKLRYDAKLILNDGLTFGKEGKKHARINVAAPRSVIEEAVLRLVTNFRKND"
     gene            complement(8516..9064)
                     /locus_tag="VBE34_001382"
     CDS             complement(8516..9064)
                     /locus_tag="VBE34_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEO7264097.1"
                     /translation="MNIETLTQKNHRSDSGRNHIVLFEPQIPANTGNIARTCAATNAP
                     LHIIRPMGFPIDDKKMKRAGLDYWDKLDVSFYDGLEEFMLSCRGKVHLISKFADKVYS
                     DENYNDDQDHYFMFGREDKGLPETFMREHAEKALRIPMNDEHVRSLNVSNTVCMIVYE
                     ALRQQSFPNLELSHTYENDKLK"
     gene            complement(9237..10589)
                     /locus_tag="VBE34_001383"
     CDS             complement(9237..10589)
                     /locus_tag="VBE34_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEO7264098.1"
                     /translation="MENHNSIKQTYGLMTTIAMIVGVVIGSGIYFKVDDILKFTGGDV
                     FLGMVILVLGSFSIVFGSLSISELAIRTSESGGIFSYYEKYVSPALAATLGLFASFLY
                     LPTLTAIVSWVAAFYTLGESSSLESQIILAAVYILALSLMNIFAKRIAGGFQSLTTFV
                     KMIPLVLIALIGAFWSDKAPQLPQHLTAIQPSNVGWSWVSGLVPLYFAYDGWTIFVNI
                     APEVKNPKKNLPLAFVIGPALILLSYLAFFYGLTQILGASFIMTTGNDAINYAANIIF
                     GPSVGRLLSFIVILSVLGVANGLLLGTMRLPQAFAERGWIKSERMANINLKYQMSLPA
                     SLTVTAVAIFWLFVHFMVTKFNLLPGSDISEIAVVFNNTSLIILYVLVLSLYLKKDIK
                     NKFTGLVSPILAILGGLILLIGSLLSNFFTVLIFQCFCLLFCLICHYIYQKNNPKTHE
                     "
     gene            10795..12144
                     /gene="trkA"
                     /locus_tag="VBE34_001384"
     CDS             10795..12144
                     /gene="trkA"
                     /locus_tag="VBE34_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEO7264099.1"
                     /translation="MRIIVVGGGKVGTALCRSLVAEKHDVVLIEKKENVLKRVTKQHD
                     IMGIVGNGANYKILEQAEVKNCDIFIAITDRDEVNMISAVLAKKMGAKETVVRMRNPE
                     YSNPYFKDKNFLGFSSVVNPELLAAQYIANTIEFPNATSVEHFANGRVMLMEFKILEG
                     NKLCHTSMSQIRKKFGNIVICAIERDGKLIIPDGDATIQVKDKIFVTGNRIEMILFHN
                     YVKNKVVKNLMVIGAGRIAYYLLNILKNTNTHVKLVELNQEQAEYFSQEFPNVPVVHG
                     DGTAKNILLEESVTNFDAVATLTGVDEENIITSMFLESIGIPKNITKVNRTSLLEIID
                     DKQLSSIITPKRIAVDHVMHFVRGRVNAQDSNLEAMHHIANDRIETLQFEIKETSKLA
                     NRSLASLKLKQNILIAAIIRNNKTIFPTGEDVLTVGDRIVVITLLKNITRTSDMLAR"
     gene            12149..13588
                     /locus_tag="VBE34_001385"
     CDS             12149..13588
                     /locus_tag="VBE34_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO7264100.1"
                     /translation="MNKSMIRFLLSKLLLIEAALLAIPLTVGLIYREPQSVMMSIVIT
                     MIILIILGLLGSLFKPKNYHIYTKEGMLIVALCWILWSFFGALPFVISGQIPNIIDAF
                     FEVSSGFTTTGATILDDVSVLSPALLFWRSFTHLIGGMGVLVFALAIMENSKNSHLEV
                     MRAEVPGPVFGKVVSKLKKTAQILYLLYLLMFAVFAVILYFAGMPFFDSIIIAMGTAG
                     TGGFAVYNDSIAHYNSPLITNLVSIGMLIFGVNFNLYYLLLLRKIKAFFGDEELKTYL
                     RIVAIATFMIALNVIGMYDNFRQGLEHIFFEVSAIITTTGFGVTDITRWPLFSQVILL
                     FLMFIGGSAGSTAGGFKVMRSLILAKIARNQVLSTLYPNRVMSLHINKSVLDKNTQHG
                     VLKYLTIYLAIFMALVLVLTLDTNDFLVVISAAASCFNNIGPLLGSNETFSFFSPFSK
                     LLLSFAMIAGRLEIYPVLLMFIPKTWSKL"
     gene            complement(13612..13863)
                     /gene="yidD"
                     /locus_tag="VBE34_001386"
     CDS             complement(13612..13863)
                     /gene="yidD"
                     /locus_tag="VBE34_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEO7264101.1"
                     /translation="MLKSFLIFLVRFYQKNISPAFPASCRYRPTCSTYMIEAIQKHGL
                     KGVLMGIARILRCHPLAHGGNDPVPDHFSLRRNKTDISD"
     gene            complement(13863..14585)
                     /locus_tag="VBE34_001387"
     CDS             complement(13863..14585)
                     /locus_tag="VBE34_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO7264102.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGMVTINGQVVNELATQVKAG
                     DLVEIEGSPIYNEEKVYYLLNKPRGVISSVSDDKGRKTVIDLLPQVKERIYPVGRLDW
                     DTTGLLILTNDGDFTDKMIHPRNEIDKVYLARVKGIATKENLRPLTRGVVIDGKKTKP
                     ARYTIIKVDHEKNRSVVELTIHEGRNHQVKKMFEQVGLLVDKLSRTQFGTLDLTGLRP
                     GEARRLNKKEISQLHNAAINKS"
     gene            complement(14575..15159)
                     /locus_tag="VBE34_001388"
     CDS             complement(14575..15159)
                     /locus_tag="VBE34_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEO7264103.1"
                     /translation="MTYLGSIEALLFVAGEDGLSLRQMAELLSLTPSALIQQLEKLAK
                     RYEEDDDSSLLLLETAQTYKLVTKDSYMTLLRDYAKAPINQSLSRASLEVLSIIAYKQ
                     PITRIEIDDIRGVNSSGAITRLIAFGLIKEAGKKEVLGRPNLYETTNYFLDYMGINQL
                     DDLIDASSIELVDEEVSLFSMDSINTEDKENNEN"
     gene            complement(15156..15863)
                     /locus_tag="VBE34_001389"
     CDS             complement(15156..15863)
                     /locus_tag="VBE34_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEO7264104.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYEVDIYDVPIVEVIEQYLAYIAT
                     LQAMRLEVAGEYMLMASQLMLIKSRNLLPKVVESNPIEDDPEMELLSQLEEYRRFKVL
                     SEELANQHQERAKYFSKPKQEVIFEDAILLHDKSVMDLFLTFSQMMSQKQKELSNSQT
                     VIEKEDYRIEDMMIVIERHFNLKKKTTLQEVFADCQTKSEMITLFLAMLELIKLHQIT
                     VEQDSNFSQVILRKEEK"
     gene            complement(15863..>16448)
                     /gene="xerD"
                     /locus_tag="VBE34_001390"
     CDS             complement(15863..>16448)
                     /gene="xerD"
                     /locus_tag="VBE34_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEO7264105.1"
                     /translation="KLTARKRKLSAVNQFLFFLYNRGTLKEFYRLQETEKITLAQTKS
                     QIMDLSNFYQDTDYPSGRLIALLILSLGLTPAEIANLKKADFDTTFNILSIEKSQMKR
                     ILKLPEDLLPFLLESLEEDGDLVFEHNGKPYSRQWFFNQLTDFLNEKNEQQLTAQLLR
                     EQFILKQKENGKTMTELSRLLGLKTPITLERYYR"
BASE COUNT         5317 a         3289 c         2504 g         5338 t
ORIGIN      
        1 atatttttaa gaaagtggtt ttttcatggt cttttctaaa gtttccacaa taacatcatc
       61 aaatactagt tcttgtttta aaacatcctt gtcagcgtat ccttctagtt gtatctcgac
      121 cgctttctta cctgcttttc gttcaataat gactaaacgt ttaacactta aatttgcact
      181 agaaagacga tttaggactt ttgatagtac ccctatcccc tcatcagcta atataaccaa
      241 gcgataacct tcttgaccat accccgcaat ttcaaggaag gctttaaaaa catccctatc
      301 cgtgacaatc ccataaagtt gtccattatc aactactggt aaaacaccga tctttcgaga
      361 catcatcaaa taaatagcgt cctcaaggct agcatactgt gaaacagtca caatatcttt
      421 aatcataata tctcgaattt ttgtcttatt aagaagatag ttcatctcat atatagaaag
      481 actggttgct ttagaaggtt gtgcttctgc cattgtccct tctgtcacta accccactag
      541 ctgatcattt tcaacaactg gcaaacgcct taagtggtgt tctctcaata aatcagcagc
      601 ttctgcaact gtagtatctg gagagacata aactaatttt tttgtcataa aatctttcac
      661 tggcatacct gactcctcta aatattatcc acctaagtaa gctttacgaa cttgatctga
      721 aactagtaat tccttacctg tccctgataa aacaaccttc ccagtctcta gaacatatgc
      781 tttatcagca atcgtcagag ctttatttgc attttgttca actaaaagaa ctgtagtccc
      841 ttgttttttg atgtcttcaa tgatattaaa aatttcttga ataaaaattg gagctaggcc
      901 cattgactgt tcatctaaaa gtaataattt aggacgactc atcaatgcac gccccatagc
      961 gagcatttgc tgctcaccac ctgataaggt tgctgcatct tggtttttac gttcttctaa
     1021 gcgaggaaaa cgatcaaaca ctttttttaa cattttttga ttttgttcac gatccttttg
     1081 aagaaaggct cccatttcta aattttccat aacagacaaa cttgagaaaa cgtggcgccc
     1141 ttctggaacc tgagctaaac cattgccaac aatttttcta gctgctaact tatgtatagg
     1201 tttccccata aaagaaatag aaccctgact tggtcttact aaaccagaaa ttgtcctcaa
     1261 aattgatgtc tttcctgcac cattggctcc aattaaggtt acaacctcac cttggttaac
     1321 ttcaaacgag acatcattca ccgcttgaat aacaccatag tgaatagata gattttctac
     1381 tttcaacatg gtcattatag ctcccccccc aagtatgctt ctataacacg tttattattt
     1441 ttaatttctt caggtgtccc gtgagcaatc agacgaccat attccaatac ataaattctc
     1501 tctgttactt gcataaccaa gttcatatca tgttcaatca gcataatagt aatatcaaaa
     1561 tcactcttta tttgactaat taactgcgtc aattctgcag tttcttgagg attcattcct
     1621 gccgcgggct catccaaaaa caatattttt ggttctgttg ctaaagcacg aacaatttct
     1681 aaacgccgtt gttttccata agggaggttc ttagcaagag catcttgata tgctttcaaa
     1741 ccaaaaatct ctaatagctc cagtgccttt ttccttaagg ctttttcact atgatagtat
     1801 ttgggtaatc gtaaaaaact agcaatagga tgtgataagt gatgattact gagtcctact
     1861 agtacattgt ctaaaacagt catattttta aaaagtcgga tattttgaaa agttctacct
     1921 aaacctaaag aagctatttt agctggtttc ctcccattta atatcttacc atcaagtgat
     1981 attgtccctt tactaggaag gtatactcct gttaagagat taaaaagcgt tgtcttaccc
     2041 gcaccgttgg ggccgattaa gccaattaat tcacctttat gtaatttcat cgaaacatca
     2101 cctacagcag ttaggccacc gaaatgttta cttaaatttt ttacttcaag aagtgccatc
     2161 taattgcctc ccttactcgt cttacgaaat agatgagata gagttaactc tttcgttcct
     2221 aataaaccgc taggtctaaa aatcataatt aaaatcaggg ctaaagcata gataatcatt
     2281 cgaacagaag cgtagtcttg tagtaacata ttcaataatc ctaataaaat tgctgctaaa
     2341 actgtccctg tcattgaacc taaaccacct aaaacaacaa taattaaaac attgacagac
     2401 atcataaatg aaaagtcttt tggcataaca gttccaacgt agccagcctg taaactacca
     2461 gcgatagaag ctaaaatagc tccaaaaaca aaaacaatta ctttcacttt tgttgtatcc
     2521 acacccatag actctgctgc aatttcatct tctcgtatag caattgtatt acgccctaag
     2581 ggactacgca aaaaattcat tgcaaggata aggctcacaa ctgccacccc ataaactaaa
     2641 gaccaggttg taaaaggcgg aatccccatg atacccgcag caccattcgt aatatcacct
     2701 ccattaacca acaaaatacg gataatctca gcaacaccta aagtagctat agctaaataa
     2761 tcacctttta gtctcaaagt aggaattcct acacctaagg caatacctcc tgcaacaaga
     2821 gttccaacaa gtatagctat caaaaaacct acataagttg gcattttaac gccgataata
     2881 gcaactgcat aagcgccaat agccatgaaa cctgcatgtc ctagtgaaaa ctgtcccgaa
     2941 caacctacaa ttaggtttaa accaactgct aaaataacat taatcccaat tgtttcaata
     3001 atttgaatat gatagaggtt aaatataccc atagatatta gcacgctaat caagagataa
     3061 cctaacacta ctatagctaa ccaagataaa attgatttca tattttcttt catgcctaga
     3121 ccttctcctt aatatttttt ccaagaattc ctgctggacg gatcaagaaa atgagaatta
     3181 aaatagcata aacgatacca tctcgaaaat ctgacacacc aagtgcagtc gctaaagttt
     3241 ctaaaatgcc aataacaaaa ccaccgatag ccgctcccgg aatgatacca ataccaccta
     3301 agacagctgc aacaaatgct ttcaaccctg gagttacccc cataagaggt tgaacagaat
     3361 tataataaag cccaatcagt actccacctg ctccagctaa agcagacccc agcgcaaatg
     3421 taaaactaat ggtacggtta acattaattc ccatcaattg tgcggcatcg ctatccactg
     3481 ataaagctct catagctttc cccattttag ttttttgaac gatgaactgt aaagtaagca
     3541 ttagtgatag cgcaatacct aaaatgatga gctgaacatt ggttatagtt atcggtccta
     3601 aattatattt aacagtatga atggcttgcg gaaatgccct agtatccgct ccaacaaggt
     3661 ataccatccc gtactccaaa agaaaagaaa caccaatagc tgtaattaac gcggcaattc
     3721 tagttgattt tctcagtgga cgataagcta aatattcaat tacaacacct aggaaagcag
     3781 accccagcat tgcaattaat aaagctagga aaaaattaag atgaagatga ttaatcaaat
     3841 aatagcccat gaaagcgccc atcatataga tgtccccatg agcaaaatta atcagcttaa
     3901 taatgccata taccatagta tatcctaagg caagtaaagc atatatggat cctaagataa
     3961 gtccattaac gagctgttga agcatattat aaccatactt tctattatat attattgtcg
     4021 aattattctg aaaaattact ttttaaaaaa ggggagctga aaattaaact cccatttttc
     4081 tagagaatac ttattttgct gaaataatgt taacgctact tgtttttcca tcctctaatt
     4141 tgacaacgta agctgattta acaacattgt gatttttatc aatagacatt ttccctgtca
     4201 cacctttaaa atctttcagc ttagctaaag cttttttaat atcaattgaa gttttagctc
     4261 ctttagcaga tttagctgcc atatacacag cgtcgtatga taaagccgag aacatggatg
     4321 gttcttcacc atatgcctta acgtaatgat catggaaagc tttagctttg gttgatcctt
     4381 gtgtagtgaa acctgtcaaa taataaacgt ttgaagctcc tacaggtgtt gccgattgca
     4441 caaatttcgg actatcaaaa ccatcaggcc caagaaccgg tttagagata cctaaatcac
     4501 gcgcttgctt aactattaat cctgtctcgg tatagtaacc tggcatcaca atagcatcat
     4561 attctttccc tttcaactta gtcaatgacg cttggaaatc agtatcacca gcggagaatg
     4621 tcatactatc aacaattttt ccactataac tttccttaaa tgattttgct accccctttg
     4681 agtaatctga tgaattatca taaaatagaa caactttttt agctttaagg ttgtctgtag
     4741 catactgaga caatagcttt ccttgataat tatcgacaaa agtagctcgg aagaaatact
     4801 gatttaaggt ttttccatca gaaccataga ctaaattgtc ttgtgtcgca gcaggtgcaa
     4861 tcattggaac tgctgctgca tttacttttg gagttgaaga tgcagctgca cccgatgttg
     4921 ctggtccgat aatgacatta gctcctttgg taaccaagtt tgtggcaacc gttgctgcct
     4981 ctgcattttc tgatttatta tcctgtgata ggacttttaa cttcttgccg ccaacccctc
     5041 cagcagcatt aatttctttt actgctaagt cagctccatt cttcattgaa tttccatatg
     5101 aagaaacgtt tcctgataat tcccagttat accctacctt aatatctttc cctatttttg
     5161 ttcctgtcga agatgctcct cctccgacat ttccacatgc tgctaaaaga actgtactag
     5221 ctaaaacaag cgctcctaaa ctaagtctct tttccatatt tttctccttt tttgaaaatg
     5281 gtaactaaaa taatacagaa ttatcagaat attgtcaata gtttttaaat tgtttttttg
     5341 aaaattatag aaactaaata aaaaagaagg acatctgaac tccttctttc taataactac
     5401 tagtaaatat cttaacgata taagtttcca acaaaatcct gatcaatatc atcaattgct
     5461 gataaccgaa catccttaac aaattttaaa tgttttatct ctttactaac gctaacaata
     5521 tcctctttat taacataaat gactagatat cttaatctac gagaatgata atgaacatct
     5581 ccgtatttat ataatttacg agcgtcacga ttatagtaaa gataaacgct cattcctaaa
     5641 cgctcttgtt tattcaataa ttttttcctt tctgcgtcat atctgtaatt actaagatta
     5701 taacataatt ccaacaaata aaaaaagagt tggtaaactc aactctttta aatctattat
     5761 tgtaaattat tattttccat aatagcatca ataaagccat aatcaagtgt ttcttgagca
     5821 ctcatccaac gatcacgctc tgcatcatca tggacttttt caatagattg accagaatta
     5881 tcagctaaga ttttttctaa agtatgacgc gtttttaaaa gatgctcagc agcgatagcc
     5941 atatcagatt gctgtgtacc tccgcctgtt ccgcccattg gttggtggat catatattct
     6001 gcattcggta acataaaacg ttttccttta gcaccacttg aagcaataat ggttcccatc
     6061 gaagcagcca tccccataac aatcgtctgt acgtccgatt taatgaagtt catggtgtcc
     6121 acaatagcaa gtccagccga tactgaacca cctggtgtat tgacataaag gtaaatatcc
     6181 tttgtattat cttgtgcatc gagaaacaat aactgtgcaa tgatactatt ggccatatta
     6241 tcctcaactt ggcctgtcaa cataataata cgatctttta aaagacgtga gtaaatatca
     6301 taagaacgtt caccacgact tgtttgttca ataactactg gaatcatatc tttctccatt
     6361 tctttttatc actgtcacta ttataatcat ttggtcaaca aaggtcaaat aataactttt
     6421 ttaagaaaag gctttctgag ttcttcagaa aagccttatt catctcaaat cttattttgt
     6481 accaaataag cgatccccag catctccgag tccaggaaca atgtagccat gttcattgag
     6541 tttttcatcc aaagcagcgg tataaatatc tacatctgga tgtgcttctt gaagtgctgc
     6601 cactccttct ggagctgcta ctaaacaaac gaatttgata ctagctgcgc cacgtttttt
     6661 tagagaatct actgctaaaa tagcagaccc acctgtagca agcattggat caactacaaa
     6721 aatctgacgt tgatcaatat cttcaggtaa cttaactaaa tattcgactg gttggaaagt
     6781 ctcttcatca cgatacatac cgatatgacc aactttagca gctggcacaa ggcttaagaa
     6841 accatcaacc ataccaatac ctgcacgtaa aattggaaca attgctaact ttttcccagc
     6901 taattgtttt tgtacagttg tcgctactgg tgtttggatt tcaacatctt ctaaaggcaa
     6961 atcacgagac acttcatagc ccattaacat cgcaatctca tcaactaact cacgaaaatc
     7021 tttagtagat gttgtagtgc gacgtaagat tgacagttta tgttgaataa gtggatgtga
     7081 aatgacttga aattttccca ttataatgac cgctttctat ttaattttga gcattaaaaa
     7141 cattctaatg cttgtttaca ttcaaagtat tataacataa aaaccctttc catgcgactt
     7201 tttttatagt ctagtcattt tttctaaagt ttgtcactaa tcgcaaaact gcctcctcaa
     7261 taacagacct aggagctgcg acattgattc tagcatgttt tttcccttct tttccaaaag
     7321 ttaaaccatc atttaatatt aatttggcat catatcgaag tttttcttga atttctaaat
     7381 gcgttaaatt ataagcagaa aaatctaacc aaacaagata agttccctct ggtttcatca
     7441 ctttgatatg agttttttgc gttaattgct cataaaggta ttctattgat ccttctaact
     7501 ccatttttag ggcctttaac cattgtttcc cttttgtaaa ggcaacttct gtagctaata
     7561 accccaatga agaaatttct tgttgattgt tagctatttg cctcttctta aaatccgagc
     7621 gtaacttttc attttcaatt attgcaaaac tatttttagt ccctgcaata ttaaacgttt
     7681 tagttgcact agataaaata acactcaatt ctttaaaact agaatcaaca gtattaaagc
     7741 tatggtgtac attatcaaat aataccaaat cttgatgtat ttcgtcagaa acgaggataa
     7801 cattatatcg tttacagata tctcctattt tttgaatttc tccttttgtc cagacacgcc
     7861 caccgggatt gtgtggacta caaaaaatat aaagctttac attattttca atgatatctt
     7921 tttctaattg tttaaagtca atctcaaaat actgattatt attaagtaaa ctatttgaaa
     7981 ctaaatgacg attattgtat tttatagttc tagcaaaggg cggatagact ggggtattaa
     8041 ttagaacagc atctccctcc tttgtgaagg cttgtatagc aactgaaatc gctggtacaa
     8101 ccccatcaat taataataat gattttttat caaattgata accatgttct tgcacttccc
     8161 aattagagat agcttggtat aaactgtctt taggactatc atagccaaaa accatttggt
     8221 gtgaaaaatc ttcaatagcc tccgacattt ctggaaagat aggaaaatcc atatccgcaa
     8281 tccaaagggg aaggatttct tgatcttttt gaaccgcctt ccattttatg gcattacttg
     8341 aaaaacgttc tggtaaactt gtgaaatcat atgtaatcat aattactcct gactattcta
     8401 cagctaaaaa tatcccttgt cgccttggtg actattttac catttgtttt tgatactata
     8461 tactaattaa cattctaaag ataaaagtga ccattttaaa cgatcacttt ctttattatt
     8521 ttaatttatc attttcataa gtatggctta attctaaatt aggaaagctt tgctgcctaa
     8581 gggcttcata aacaatcata caaacagtgt ttgaaacatt caagctacgc acatgctcat
     8641 cgttcatggg aattcgtaag gccttttcgg catgctcgcg cataaaagtt tctggcaatc
     8701 ctttatcttc acgaccaaac ataaaataat ggtcttgatc atcattgtaa ttttcatctg
     8761 agtaaacctt gtcagcaaat ttgctaatta ggtgaacttt ccctcgacat gacaacataa
     8821 attcttccaa accatcatag aaactaacat ctagcttatc ccagtaatcc agtcccgctc
     8881 gtttcatttt tttatcatca ataggaaatc ccatcggtcg tatgatgtgc aaaggagcat
     8941 ttgttgccgc acaagttcta gcaatattac cagtatttgc tggaatctgt ggttcaaata
     9001 aaacaatatg atttcgtccg ctatcggaac gatgattttt ctgcgttagt gtttcaatat
     9061 tcatctcatc atcactttct agaactcatt ctgactcgca aaaaaaccac actgctcgga
     9121 gtcaatctca gcaaacaatg tggtttatgg tggtttgtta acttaaatca taacaggttt
     9181 gatttaaact aacaaatcgt tgacactatt atagcacttc agtctacaaa gtcaacctat
     9241 tcatgagttt ttgggttatt tttttgatag atataatgac aaatcaaaca gaataataag
     9301 caaaaacatt ggaaaattaa aaccgtaaag aaattactta acaaactccc gataaggagg
     9361 atcaaaccgc ctaaaattgc tagaatcggt gaaactaagc cggtaaattt atttttaata
     9421 tcttttttga gataaaggct gagtactaaa acataaagaa tgattaaact ggtattatta
     9481 aacaccacag caatttcact aatatcacta cccggcaata agttaaattt tgtaaccata
     9541 aaatgaacaa aaagccaaaa aatagctaca gccgtcaccg tcaaactagc tggcaatgac
     9601 atttgatact tcaaatttat gttagccatc ctctcacttt tgatccatcc gcgttccgca
     9661 aaagcttgag gtagacgcat cgtcccaagt aaaagtccat tggcaacacc taatacagat
     9721 agaataacaa tgaaagataa taaacgccca acactaggac cgaaaataat attagctgca
     9781 taattaatag catcattacc ggttgtcata ataaagctag cacctaaaat ttgtgtcaaa
     9841 ccatagaaaa atgccaaata tgataataag ataagcgctg gaccaatgac aaaggcaagt
     9901 ggtaaattct tcttgggatt cttgacttct ggtgcaatat taacaaaaat tgtccaacca
     9961 tcatatgcaa aatacaatgg taccagacct gacacccaac tccatccaac atttgacggt
    10021 tgaatagctg ttaaatgttg tggaagttgt ggagctttat cagaccaaaa agcaccaatt
    10081 aaagcaatta gaactaaagg aatcattttt acaaaggtcg ttaaagattg gaatccccct
    10141 gcaatacgct tagcaaagat attcatgaga ctaagcgcta aaatataaac agcagctaaa
    10201 ataatctgtg attccaaact tgatgattca cctaaagtat aaaatgcagc tacccatgaa
    10261 acgattgctg ttaaagttgg aagatacaaa aaagacgcaa acaagcctaa agttgctgct
    10321 aaagctgggc taacatattt ttcgtagtaa gaaaaaatac caccactttc actcgttcta
    10381 atagctagct cagaaattga caaacttcca aaaacaatcg aaaaagaacc tagtactaaa
    10441 attaccatgc ctaaaaagac atctccacca gtaaatttaa ggatgtcatc aactttaaaa
    10501 taaatgcctg aaccaatcac tacacctaca atcatagcaa ttgtagtcat taatccatag
    10561 gtctgtttaa tagaattatg attctccata atatctccat ttctactgta cgcttaaagg
    10621 ttaattataa cctaatttag ggcttgtcac aagtttatct gtgttatttt tcaccagatt
    10681 ctgataagag aatcatatta ctcttatcag aaagatgaaa acgtttttta accataattc
    10741 tcgtgcattt ttactaaaaa aaaggtatca tagagtttat tattggaggt aaaaatgaga
    10801 ataattgttg tcggcggagg taaagtcgga accgcacttt gtcgctcact tgttgctgaa
    10861 aagcacgatg ttgttttaat tgagaaaaaa gaaaacgtcc ttaaacgtgt cactaaacag
    10921 catgatatta tgggaattgt tgggaatgga gcaaactata aaattttaga acaagccgaa
    10981 gtcaaaaatt gtgatatttt tatcgctatc actgatcgag atgaagttaa tatgatttca
    11041 gcagttcttg ccaaaaaaat gggggcaaaa gagactgttg ttcgcatgcg taatcctgag
    11101 tattctaatc cttacttcaa agataaaaac tttcttggct tctcttcagt tgtcaaccct
    11161 gaattgttag cagcacaata tatcgctaat accatagaat ttccaaatgc aacttcagtt
    11221 gaacacttcg ccaacgggcg tgtgatgtta atggaattta aaattttaga aggaaataaa
    11281 ctctgtcata cttcaatgag tcaaattcgt aaaaaatttg gtaatattgt tatttgcgca
    11341 attgaacgcg atggcaaatt gattattcct gatggcgatg caactatcca agtcaaagat
    11401 aaaatctttg taactggaaa tcgtattgaa atgatacttt tccataatta cgttaaaaat
    11461 aaagttgtca aaaacttgat ggttatcggt gcaggacgca tcgcctacta cctactcaat
    11521 atccttaaaa ataccaatac acacgtcaaa ctagttgaac tcaaccaaga gcaagctgaa
    11581 tattttagtc aagaatttcc caatgttcct gtcgttcatg gggatggtac tgctaaaaat
    11641 attttgttag aagaaagcgt tacaaatttt gacgcagttg ctactttgac tggtgttgat
    11701 gaagaaaata tcattacctc aatgttctta gaaagtatcg gtatccctaa aaacattacc
    11761 aaagttaatc gaactagcct actagaaatc attgacgata aacaactatc aagcattatc
    11821 acacccaagc gtattgccgt agaccatgtg atgcactttg tacgcgggcg tgtaaatgct
    11881 caggattcca acctagaagc tatgcatcat attgctaacg atcgcattga aaccttacaa
    11941 tttgaaataa aagaaactag caaattagca aatcgtagcc ttgcatcttt aaaattaaag
    12001 caaaatattt taattgcagc gattattcgc aataataaaa cgattttccc aactggtgag
    12061 gatgttttaa cagtgggtga tcgtatcgtt gttatcacct tattaaaaaa tattactaga
    12121 actagtgata tgttagcgag gtaggaatat gaataaaagt atgattcgtt tccttctctc
    12181 aaaacttttg ctaattgaag ctgctcttct agcgattcct ctaactgttg gacttattta
    12241 ccgtgaaccg caaagtgtca tgatgagtat agtcattacc atgattattt taatcatcct
    12301 tggattactg ggttctctct ttaaacctaa aaattatcat atctacacta aagaaggcat
    12361 gttaattgtt gcactctgct ggatactttg gtccttcttt ggcgcccttc catttgttat
    12421 ttcagggcaa atccctaata ttatcgatgc tttctttgaa gtatcttctg gttttacgac
    12481 aacaggagca actattcttg atgacgtcag tgtcttatca cccgcgctct tattttggcg
    12541 tagctttacc catcttatcg gtggaatggg ggttttagta tttgcccttg ccatcatgga
    12601 aaattctaaa aatagccatt tagaggttat gagagctgaa gttcctggac ctgtatttgg
    12661 taaagttgtc tcgaaactta aaaaaactgc acaaattttg tatttgttat atcttcttat
    12721 gtttgcagta tttgcagtaa tattatattt tgcaggtatg cctttctttg atagtattat
    12781 catagcgatg ggaactgcag gtacaggtgg ttttgcagtt tacaatgata gcattgctca
    12841 ttataatagt cctctgataa ctaacttagt ttctattggt atgcttatct tcggggttaa
    12901 ctttaatctt tactatcttc ttttactccg aaaaattaaa gcattctttg gtgatgagga
    12961 actaaaaacc tatctccgca tcgtggcaat agcaactttc atgattgctc taaacgtcat
    13021 cggtatgtat gataattttc gacaaggcct cgaacatatt ttctttgaag tatcagctat
    13081 tattacgaca actggttttg gcgtgactga tatcactcgt tggcctttgt tttcacaggt
    13141 tattttgcta ttcttgatgt ttatcggtgg ttctgctggc tctacagctg ggggctttaa
    13201 agtcatgcgt agcttaatcc tagcaaagat tgcacgtaac caagtattgt ctacactcta
    13261 tcctaataga gtcatgtcac tacacattaa taaaagcgtt ctagataaaa atacacaaca
    13321 tggtgtttta aaatatctaa ctatctattt agctatcttc atggcactcg ttcttgtttt
    13381 aacgcttgat actaatgatt ttttggttgt tatcagtgct gcagcctcat gttttaacaa
    13441 cataggccca ctcttaggta gcaatgaaac tttctcattc tttagtccat tctcaaaact
    13501 ccttctttca tttgctatga tagcaggacg tttagaaata taccctgttt tattgatgtt
    13561 tattcctaaa acatggtcaa aactataatt acaaataata aaacggatat atcaatctga
    13621 tatatccgtt ttattacgtc ttaagctaaa atgatcaggg acaggatcat ttcctccgtg
    13681 ggctaaggga tgacatcgca aaatacgtgc aatccccatc aacacacctt ttagaccatg
    13741 tttttgaata gcttctatca tatacgtaga gcaagttgga cgataacgac agctagctgg
    13801 gaaagctgga gaaatatttt tttggtaaaa gcgaactaag aaaatcaaaa aagatttaag
    13861 cattaagatt tattaatagc cgcattatgc aactgactaa tttctttttt atttaatcgt
    13921 cgtgcttccc caggacggag acccgttaaa tctaaggtac cgaattgtgt acgagatagc
    13981 ttgtccacta ataacccaac ttgttcaaac attttcttaa cttgatgatt acgaccttca
    14041 tgaatagtta attcaactac tgaacgattt ttttcatgat ccacttttat gatagtatag
    14101 cgagcaggtt ttgttttctt accatcgata acaacgccac gcgttaatgg tcgaaggttt
    14161 tctttagtag ctattccttt aacacgtgct aaataaactt tatcaatttc attacgagga
    14221 tgaatcattt tatcagtaaa atcaccatca ttagtcaaaa ttaaaagtcc agttgtatcc
    14281 caatccaagc gaccaacagg atatatccgt tctttgactt gtggcaacaa gtcaataact
    14341 gtcttacgtc ctttatcatc tgagacactt gaaataacac cacgtggttt atttaagaga
    14401 taatatacct tctcttcgtt gtaaattgga cttccttcaa tttcaaccag atcaccagcc
    14461 tttacttggg tcgccaattc attaaccact tgaccattaa tcgtgaccat tccttgcttg
    14521 attaattcct cagcttttct acgacttgca atccctgcat gcgcaatata tttattaatt
    14581 ctcattgttt tccttatctt ctgtattaat tgaatccatt gaaaataacg atacttcttc
    14641 gtcaactaat tcaattgaag aggcgtcaat taagtcatct aactgattaa ttcccatata
    14701 atctaagaaa taattagttg tctcataaag gttaggacgt cccaacactt ctttctttcc
    14761 agcctcttta attaaaccaa aagctatcag tctcgttata gctccactgg aattcacgcc
    14821 acgaatatca tcaatttcaa tcctagtaat aggttgttta taagcaataa tagatagaac
    14881 ttccaaactt gcccgagata gactctgatt aatcggagcc ttcgcataat ctctcaacaa
    14941 agtcatataa ctatcctttg ttaccaattt ataagtttgt gctgtctcaa gcaataatag
    15001 actagaatcg tcatcttctt catatcgttt agccaacttt tctagttgct gtattaatgc
    15061 tgatggtgtc aaagacaata attctgccat ttgacgcaaa ctcagtccat cttcacctgc
    15121 aacaaaaaga agagcttcta ttgagcctaa ataagtcatt tctcttcctt tcttaagata
    15181 acttgactaa aattactgtc ttgttctact gtgatttgat gtaattttat caactctaac
    15241 attgccaaaa ataaagttat catttcagat tttgtttggc aatccgcaaa cacttcttgt
    15301 aatgtcgttt tctttttaag attaaaatgt ctttcaataa cgatcatcat atcttcaata
    15361 cggtagtctt ctttctcaat taccgtttga ctatttgaca attctttttg tttttgggac
    15421 atcatttgag aaaaagtcaa aaaaagatcc atcaccgatt tatcgtgtag aagaattgca
    15481 tcttcaaaga taacttcttg ttttggtttt gaaaaatatt tggcgcgttc ctgatgctga
    15541 ttagctaact cttcacttag cactttaaaa cgtctatatt cctctaattg gctcaacaat
    15601 tccatttctg gatcatcttc aataggattt gactccacta ctttaggtaa taagttacgg
    15661 ctttttatca acatcagctg acttgccata agcatatact caccagctac ttctagacgc
    15721 atcgcctgta atgttgctat ataagcaagg tactgctcaa taacttccac aatcggtaca
    15781 tcgtatatat caacctcata tttagatacg aggtgcaaga gtaagtctag gggcccctcg
    15841 aaatctttta acttaatatc cattatctat aatatctctc taaagtgata ggagttttta
    15901 agcctagaag gcgactgagc tccgtcatcg ttttgccatt ttctttttgc tttagaataa
    15961 attgttctcg cagaagttgt gcagttagtt gttgctcatt cttctcattt aagaaatctg
    16021 ttaattgatt aaaaaaccat tgtctagaat aaggcttacc gttatgctca aagactaagt
    16081 caccatcttc ctctaaagat tccaaaagaa aaggaagcaa gtcttctgga agttttaaaa
    16141 tacgcttcat ctgcgatttt tcaatactta agatattaaa agttgtatca aaatcagcct
    16201 tcttcaaatt agcaatttca gccggagtta aacctaaaga taaaatcaac aaagcaatca
    16261 aacgaccact cggataatca gtatcttgat agaaatttga taaatccatt atttgagact
    16321 ttgtctgagc caaagtgatt ttttcagtct cttgtagtct gtaaaattcc ttcagcgttc
    16381 ctctattata taaaaagaaa agaaattgat tgactgcaga taactttcgc ttacgagctg
    16441 ttaactta
//
LOCUS       DATRUK010000045        16363 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.045, whole genome shotgun sequence.
ACCESSION   DATRUK010000045 DATRUK010000000
VERSION     DATRUK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16363
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.045"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..305)
                     /locus_tag="VBE34_001391"
     CDS             complement(<1..305)
                     /locus_tag="VBE34_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome assembly protein"
                     /protein_id="HEO7264106.1"
                     /translation="MLHSKKIHSLSLIAVLSLATYTSLQPNHVAAEQSQKTSTVHMSQ
                     KTIEHKLKVADKEAAPLYAKIDHIQRHIEVKKAKDLKVIELYINKDINQLEKQNKRLL
                     "
     gene            complement(398..1786)
                     /locus_tag="VBE34_001392"
     CDS             complement(398..1786)
                     /locus_tag="VBE34_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7264107.1"
                     /translation="MKKKSLISNLILINSITLFLGFYLIYFSVNYVIKDYVHQMTRNS
                     MQANFTILDSIYNNKKIPDENMVNQDSIFVWSYYAIFDQHYRMKYGTDNHKKESHDII
                     RYLTQKRLWQISKEKDGMFITIKKKTYYVMTKDYSGILVDGSIKKAPKAQSQLFHVIN
                     FSDITYTQHLITKINHFLIVILVLTYIPMLFIMRKTFTGIRESIQSVQTYISSLWKNQ
                     GNHQSSQKEIVFSDFDPLLLESQEMANRIYQAEESQRNFFQNASHELRTPLMSIQGYT
                     EGVQEGIIDAELAHSVILQESKKMKQLVDDIILLSKLDSNLSDQKDEFSLNELLNSII
                     AYFKPLANKQKISITYRPDKHEKLLKGNEELIQRAINNILSNALRYAVSHIEISYTNQ
                     KLTISNDGPAISKEDLPYIFDRFYKGHGGQTGIGLAMTKEIIKQHHGNIIAESDSTST
                     TFTIYFNCHKIA"
     gene            complement(1783..2468)
                     /locus_tag="VBE34_001393"
                     /pseudo
     CDS             complement(1783..2468)
                     /locus_tag="VBE34_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161341.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(2701..7416)
                     /locus_tag="VBE34_001394"
     CDS             complement(2701..7416)
                     /locus_tag="VBE34_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO7264108.1"
                     /translation="MNTKQRFSIRKYKLGAVSVLLGTLFFLGGITNVAADSVINKPSD
                     IAVEQQVKDSPTSIANETPTNNTSSALASTAQDNLVTKANNNSPTETQPVTEPHSQAT
                     ETFSPAANQPVESTQEVSKTPLTKQNLAVKPTPAISKETPQNIDSNKIITVPKVWNTG
                     YKGEGTVVAIIDSGLDTNHDALQLNDSTKAKYQNEQQMNAAKAKAGINYGKWYNNKVI
                     FGHNYVDVNTELKEVKSTSHGMHVTSIATANPSKKDTNELIYGVAPEAQVMFMRVFSD
                     EKRGTGPALYVKAIEDAVKLGADSINLSLGGANGSLVNADDRLIKALEMARLAGVSVV
                     IAAGNDGTFGSGASKPSALYPDYGLVGSPSTAREAISVASYNNTTLVNKVFNIIGLEN
                     NKNLNNGLAAYADPKVSDKTFEVGKQYDYVFVGKGNDNDYKDKTLNGKIALIERGDIT
                     FTKKVVNAINHGAVGAIIFNNKAGEANLTMSLDPEASAIPAIFTQKEFGDVLAKNNYK
                     IVFNNIKNKQANPNAGVLSDFSSWGLTADGQLKPDLSAPGGSIYAAINDNEYDMMSGT
                     SMASPHVAGATALVKQYLLKEHPELKKGDIERTVKYLLMSTAKAHLNKDTGAYTSPRQ
                     QGAGIIDVAAAVQTGLYLTGGENNYGSVTLGNIKDKISFDVTVHNINKVAKDLHYTTY
                     LNTDQVKDGFVTLAPQQLGTFTGKTIRIEPGQTKTITIDIDVSKYHDMLKKVMPNGYF
                     LEGYVRFTDPVDGGEVLSIPYVGFKGEFQNLEVLEKSIYKLVANKEKGFYFQPKQTNE
                     VPGSEDYTALMTTSSEPIYSTDGTSPIQLKALGSYKSIDGKWILQLEQKGQPHLAISP
                     NDDQNQDAVALKGVFLRNFNNLRAKVYRADDVNLQKPLWVSAPQAGDKNYYSGNTENP
                     KSTFLYDTEWKGTTTDGIPLEDGKYKYVLTYYSDVPGSKPQQMVFDITLDRQAPTLTT
                     ATYDKDRRIFKARPAVEHGESGIFREQVFYLKKDKDGHYNSVLRQKGEDGILVEDNKV
                     FIKQEKDGSFILPKEVNDFSHVYYTVEDYAGNLVSAKLEDLINIGNKNGLVNVKVFSP
                     ELNSNVDIDFSYSVKDDKGNVIKKQHHGKDLNLLKLPFGTYTFDLFLYDEERANLISP
                     QSVTVTISEKDSLKDVLFKVNLLKKAALLVEFDKLLPKGATVQLVTKTNTVVDLPKAT
                     YSPTDYGKNIPVGDYRLNVTLPSGYSTLENLDDLLVSVKEGQVNLTKLTLINKAPLIN
                     ALAEQTDIISQPVFYNAGTHLKNNYLANLEKAQTLIKNRVEQTSIDNAIAALRESRQA
                     LNGKETDTSLLAKAILAETEIKGNYQFVNASPLSQSTYINQVQLAKNLLQKPNVTQSE
                     VDKALENLDIAKNQLNGHETDYSGLHHMIIKANVLKQTSSKYQNASQFAKENYNNLIK
                     KAELLLSNRQATQAQVEELLNQIKATEQELDGRDRVSSAENYSQSLNDNDSLNTTPIN
                     PPNQPQALIFKKGMTKESEVAQKRVLGVTSQTDNQKIKTNKLPKTGESTPKITYTILL
                     FSLSMLGLATIKLKSIKRE"
     gene            7739..8431
                     /locus_tag="VBE34_001395"
     CDS             7739..8431
                     /locus_tag="VBE34_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEO7264109.1"
                     /translation="MEKGFKEGVKDALPTALGYISIGLAFGIVASASDLSAIEVGLMS
                     ALVYGGSAQFAMCALLLAKADLMTITMTVFLVNLRNMLMSLHATTIFKSAHLMNQLAI
                     GTLITDESYGVLLGEALHHKVVSPSWMHGNNVMSYLTWVISTIIGTLLGSTIPNPEMF
                     GLDFALVAMFIGLFVFQLFGMLSDGKRLVVYVLASVGLSYFLLSTFLSGALSVLLATV
                     VGCSVGVVLDDK"
     gene            8421..8744
                     /locus_tag="VBE34_001396"
     CDS             8421..8744
                     /locus_tag="VBE34_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEO7264110.1"
                     /translation="MINSTVFLAILLGFVVSWVPRVAPFIFVKYKGLPNSLVKFLKFL
                     PLSILFALTFSSFFSVKLGQLPTLKWLDLLAGIPTLVVTIRYRNLFYTVLTGIVTMAL
                     LRLLF"
     gene            9114..11351
                     /gene="metE"
                     /locus_tag="VBE34_001397"
     CDS             9114..11351
                     /gene="metE"
                     /locus_tag="VBE34_001397"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEO7264111.1"
                     /translation="MVKVSNLGYPRLGEQREWKQAIEAFWAGNLEQKDLEKQLKQLRI
                     NHLKKQKEAGIDLIPVGDFSCYDHVLDLSFQFNVIPKRFDEYERNLDLYFAIARGDKD
                     NVASSMKKWFNTNYHYIVPEWEVETKPHLQNNYLLDLYLEAREVVGDKAKPVITGPIT
                     YVSLSSGIVDFEATVQRLLPLYKQVFQDLIDAGATYIQIDEPIFVTDEGELLVDIAKS
                     VYDFFAREVPQAHFIFQTYFESAVCLDKLSKLPVTGFGLDFIHGRAENVAAVKQGLFR
                     EKELFAGIVNGRNIWAVNLEETLALLEEIGPFVKRLTLQPSSSLLHVPVTTKYETHLD
                     PVLKNGLSFADEKLKELELLASAFDGNKTKGYHEALSRFSALQAADFRHVALESLAEV
                     RLERSPYKLRQALQAEKLQLPILPTTTIGSFPQSPEIRKKRLAWKRENLSDSDYKDFI
                     KTEIRRWIAIQEDLDLDVLVHGEFERVDMVEFFGQKLAGFTTTKLGWVQSYGSRAVKP
                     PIIYGDIKHIQPLSLEETVYAQSLTKKPVKGMLTGPITITNWSFERDDISRSDLFNQI
                     ALAIKDEIQLLEQSGIAIIQVDEAALREGLPLRQQKQQAYLDDAVAAFKIATSSVKDE
                     TQIHTHMCYSKFDEIIDSIRSLDADVISIETSRSHGDIIESFETAVYPLGIGLGVYDI
                     HSPRIPTKEEIIVNIQRSLKCLSKEQFWVNPDCGLKTRREAETIAALEVLVSATKEVR
                     QQLDN"
     gene            11396..13240
                     /locus_tag="VBE34_001398"
     CDS             11396..13240
                     /locus_tag="VBE34_001398"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEO7264112.1"
                     /translation="MSKFLEKLKTDILVADGAMGTLLYTYGLDTCHESYNVTHPEKVL
                     AIHQAYIEAGADVIQTNTYGAQRHRLKNYGLEDQVVSINQAAVNIAHQATLGKETFIL
                     GTVGGFRSQRQCDLTLDNIVEETLEQVEALLVTGQLDGLLFETYYDIEEITTVLKIVR
                     EMTDLPIITNISLHEAGVTSNGKPIVEALSQLVMLGADVIGLNCHLGPYHMIQSLKQV
                     PLFAQSYLSVYPNAGQLSLDGENSQYQFSQNSEYFGKSAELLVAEGVRLIGGCCGTTP
                     DHIRAVKRSIRGLKPIERKVVTPIIPVKDFVRRIRRTDTLVDKVKKEVTIIAELDPPK
                     HLDIVQFQKAIRAIDQKGIAAITLADNSLSNTRICNLSIASLLKDEISTPFLLHIACR
                     DHNLIGLQSRLLGMELLGFNHILAITGDPTKLGDFPGATSVYDVTSFKLLSLIKQLNQ
                     GLSYSGASLRRPTDFTVAAAFNPNVKNLTRTVKLIEKKVASGADYFMTQPIFDHSVLR
                     ELADLTKTVEQPFFIGIMPITSYNNAVFLHNEVPGIKLSESFLSALEKVKDDKEACLT
                     LALNESKSLIDEALNHFNGIYLITPFLRYDLTLELIDYIQKKQVRKSS"
     gene            13587..14294
                     /locus_tag="VBE34_001399"
     CDS             13587..14294
                     /locus_tag="VBE34_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEO7264113.1"
                     /translation="MVCVDFIDQKVYSEAEGLFVSDLDLGIKGLIRARYPFALDSDFI
                     SYGNLSHYCMNYLDEIVQRANQKNEKIKYNVTTLMKEEEKPFNVEERLNKQATIGQRI
                     ADDVARFGGSWTFIIVFVSIMAIWMLVNIMKPFGIQFDPYPFILLNLALSTIAAIQAP
                     LIMMSQNRAADYDRLQARNDFNVNKTSELEIRLLHEKIDHMVQQDQFELLEIQKLQTE
                     MLVSLGNQLAQLKQLQK"
     gene            complement(14459..15544)
                     /locus_tag="VBE34_001400"
     CDS             complement(14459..15544)
                     /locus_tag="VBE34_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO7264114.1"
                     /translation="MTTNVMANYSYGEDCFKEIPSVLATYRIQSIAFVGGKRALDSSE
                     AEVRSILEEANIKVTGSFVYGTDSTQSNIDKLVANPQVQAADAILGFGGGKALDTAKM
                     VAKELGKNSFTIPTICSNCSAGTAIAVVYNDDHSFLRYGYPESPLHIFINTRIIAQAP
                     SKYFWAGIGDGISKAPEVERATLEAKTNKLPHTAVLGQAVALSSKEAFYQFGEQGLKD
                     VEANLASRAVEEIALDILISTGYASNLVNQPDFYYNSCHAHAFYYGTTAIQRQGEFLH
                     GVVVAFGVLVLHAYFNELEELEKVARFNKSLGLPTTLADVSLSEKDIPKIVEIAMTTN
                     EYKNTPFDPKMFAQAILAADAFGQTLA"
     gene            complement(15745..16284)
                     /locus_tag="VBE34_001401"
     CDS             complement(15745..16284)
                     /locus_tag="VBE34_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEO7264115.1"
                     /translation="MKIAIIGYSGSGKSTLARKLGNYYNCNVLHLDSIHFAPNWEERK
                     YDDMIDDVSSMLEKRTWIIEGNYKKLLYQERLADADEIIFFDFNRFNCLWRAFKRYCK
                     FRGKTRPDMANGCPEKLDFEFISWILKDGRSDKQKSNYKQVVEDYPQKIKVLKHQRDL
                     DQYLKELPVASNTDPLKSL"
BASE COUNT         4934 a         2677 c         3068 g         5684 t
ORIGIN      
        1 agtagacgtt tattttgctt ctctagttgg ttgatatctt tgttaatata caattcaata
       61 acttttaaat cttttgcttt tttgacttca atatgtcgtt ggatatggtc gattttagcg
      121 tagagaggag cagcttcttt atctgcaact tttaacttat gttcaatagt tttttgactc
      181 atatgaacag ttgatgtttt ttgtgattgt tcagccgcta catgatttgg ttgtaaactc
      241 gtatatgttg ctaaagagag aacggcaata agcgataagg aatgtatttt tttagaatgt
      301 aacatataaa aactcacttt cttttagaat aactttttga tctataggta ttataggtta
      361 aaaaagcaat aacttctttg aatttttgta ggaaaagtta ggcaattttg tggcaattga
      421 aataaatcgt aaaagttgtt gaggtcgaat cactctcagc gatgatatta ccatgatgtt
      481 gcttgataat ttctttagtc attgcaagtc caatacctgt ttggccgcca tgtcctttat
      541 agaagcgatc gaaaatataa ggcaagtctt cttttgaaat ggctggtccg tcgtttgaga
      601 tagttagttt ttgatttgtg taagagattt caatatgaga aacagcatag cgtagtgcgt
      661 tactaaggat attattgata gctcgttgaa tcaactcttc gttacctttt agtaactttt
      721 catgcttgtc tggtctataa gtgatactaa tcttttgttt attagctagg ggtttgaaat
      781 aagcaataat actattaagt aattcattta aagaaaattc atctttttga tcacttaagt
      841 tactatctaa tttagagagt aagataatat cgtctacgag ctgtttcatt tttttgctct
      901 cttgcagaat aacagagtgt gcgagctcgg catcaataat accctcttga actccctcag
      961 tatatccttg gatggacatt aaaggtgtcc gtaactcgtg ggaggcattt tgaaagaagt
     1021 ttctttggct ctcttctgcc tggtagattc gatttgccat ttcttggctc tcaagtagaa
     1081 gaggatcgaa atcagaaaag acaatttctt tttgactaga ttgatgattg ccttgatttt
     1141 tccaaaggct ggaaatataa gtttgtactg attgaataga ttctctaatt ccagtgaaag
     1201 tcttcctcat aataaatagc atgggaatat aggttagtac taagataact ataagaaaat
     1261 gattaatttt agtaattaga tgctgggtgt aagtaatgtc tgaaaagtta atcacatgaa
     1321 aaagttgcga ctgtgcttta ggagcttttt ttatgctacc atcaacaagg ataccagagt
     1381 agtctttggt cattacataa taggtttttt tcttgatggt tataaacata ccatcttttt
     1441 ccttggaaat ttgccacaat cttttttggg taagatagcg aataatatca tggctctctt
     1501 ttttatgatt atcagtgcca tatttcatac gataatgctg gtcaaaaata gcatagtaag
     1561 accaaacaaa gatgctatct tggttaacca tattttcatc tggtattttc ttgttgttat
     1621 agatactatc taaaatggtg aaattcgcct gcatggaatt ccttgtcatt tgatggacat
     1681 agtctttaat gacatagttg acagaaaaat agatgaggta aaaaccaaga aatagagtga
     1741 tgctattaat aaggataaga ttgctaatta aacttttctt tttcatttgt cctctttgat
     1801 taatttgaag ccgtaacccc agacagtttt gatactaacc tgactgtggt attgcttaag
     1861 ttttttacgt aatcgtttaa tagtgtcatc aactgctctg ctttcaatat catcaaatcc
     1921 ccaaatacga ttaagcagtg tttccctaga aaaagcagtt tcaggttttt caattaatac
     1981 taaaagaaaa tcaaattctg tcttggttaa gggcatttct tcatttgata atagggctat
     2041 tcgcttttca gtatccagaa ttaaatctag aactgatact gagtgtcatt tttgattgct
     2101 ttttcatcaa ggcgtttgaa caatgcttta acatgtagac taagttttat aggtgaaaaa
     2161 ggcttggtga agtaatcgtc agttcctaaa ttaaaagccg taatgaaatc aacatcagaa
     2221 tctctagctg ttaacatgat aataggaata ttatcatatt ggcggatgct tttcataaca
     2281 tctaaaccat tagttccagg catcattatg tctaatataa ctaaatcggg tttttggttt
     2341 ttataggctt ctaagagaag gtctccagtt tcaaatgctc gaacagtgaa accatcatgt
     2401 tctaaaaaag gaaccaccaa gtcacgaata ttcttttcat catcagcgaa ataaattatt
     2461 tttgtcatgt atacctcaat acccatagtt ataatttgac ttttagctag ctgtttatgt
     2521 tatatcaaat ataacaaaaa aagagttttt ttcctattct ttgaagtgtt atagtgttat
     2581 aaaccaagat attgcagaga aaaggtcttt aggaacatca ttttaagctc ctcaaaaagg
     2641 caccagtcac taactattaa tagctagtaa ttgatgcctt gctctatttt ttaaagtatt
     2701 ttattctctt ttgatagact ttagtttgat tgttgccaga cctagcatag agagactaaa
     2761 tagcaatatt gtataggtta ttttaggagt gctttcgcct gttttaggaa gcttgtttgt
     2821 ctttatcttt tgattatcgg tttggctagt cacccctaag acacgcttct gagcaacctc
     2881 actttcttta gtcatgcctt ttttgaatat caacgcctgg ggctgatttg gcggattgat
     2941 aggtgtggta ttgagagagt cattatcatt gagtgattga ctataattct ctgcggaaga
     3001 aactctatcg cgaccatcaa gttcttgttc ggttgctttt atttggttta ataactcttc
     3061 aacttgagct tgtgtagctt gtctattgga aagcagcaat tctgctttct tgataaggtt
     3121 attataattt tctttagcaa attgactggc gttctgatat ttagatgatg tttgtttcag
     3181 aacatttgct ttaattatca tatggtgtaa accagagtaa tcagtttcat gaccatttaa
     3241 ttgattttta gcaatatcaa gattttctaa ggctttgtct acttctgatt gagtgacgtt
     3301 aggtttttgt agaagatttt tcgccaattg gacttgattg atataagttg attggcttaa
     3361 tggactagca ttaacaaatt gatagtttcc cttgatttct gtttcagcta aaatagcttt
     3421 tgccagtaaa gaagtatctg tttctttacc gttaagagct tggcgacttt ctctcaaagc
     3481 agcaatagca ttatcaatac ttgtttgttc cactctattt ttaattaaag tttgtgcctt
     3541 ttcaagatta gctaggtaat tattttttaa gtgagttcca gcattataaa acacaggttg
     3601 ggaaataata tcagtttgtt ctgctagggc attaattaga ggagctttat taatcagcgt
     3661 caattttgtt agatttactt gaccttcttt tacggataca agtaaatcat ctaagttctc
     3721 taaagtgcta tacccactag gcagcgttac gtttaaacga tagtctccta caggtatgtt
     3781 tttaccatag tcagtaggag aataagttgc ttttggtaga tcaacaacag tatttgtctt
     3841 agtaaccaac tggactgttg ctccttttgg taaaagcttg tcaaattcaa cgagtaaggc
     3901 tgctttcttg agtaagttaa ctttaaataa gacgtcttta aggctatctt tttcagaaat
     3961 agttacagtg acactttggg gactgattag atttgctcgt tcctcatcgt ataagaatag
     4021 gtcaaacgta taggtaccaa aaggcaattt cagtaaattg aggtctttcc cgtgatgttg
     4081 ctttttgatg acattacctt tgtcatcttt gacagagtaa gagaaatcaa tatcgacatt
     4141 actgttaagc tcagggctaa acactttgac gtttactaaa ccatttttat tgccaatatt
     4201 gatcaaatct tcgagttttg ctgacactag attgcctgca taatcttcaa cagtgtagta
     4261 gacatgagag aaatcattaa cctctttagg tagaataaag ctaccatcct tttcttgttt
     4321 gataaatact ttgttatctt caacaaggat accgtcttct cctttttgac gtaagacgct
     4381 attataatga ccatctttat ctttttttaa gtaaaaaact tgttctctaa agataccaga
     4441 ttccccgtgt tctactgcag gacgagcttt gaagatacgt ctatctttgt cataagttgc
     4501 tgttgttagt gtaggagctt gtctatccaa agtgatatca aataccattt gttgtggctt
     4561 agagccaggg acatcagagt agtatgttaa aacgtatttg tattttccat cttctaaagg
     4621 aataccatca gtagtggttc ctttccattc tgtgtcatat aaaaatgtag attttggatt
     4681 ttcagtattt ccgctatagt aatttttatc tcctgcttgg ggagcactta cccatagtgg
     4741 tttttgtaaa ttaacatcat ctgcacgata gactttggct cttaaattat tgaaattacg
     4801 taagaaaaca cctttcagtg caacggcatc ttgattttgg tcatcattag gtgaaatggc
     4861 tagatgaggc tggccttttt gctctagttg taagatccat tttccatcta tagacttata
     4921 gcttcccaag gctttcaatt ggataggact agtaccgtct gttgagtaga taggctctga
     4981 acttgtagtc attaaggcag tataatcttc tgaaccagga acttcgtttg tttgtttcgg
     5041 ttggaaataa aatccctttt ctttgttagc aacaagctta taaatggatt tttctaaaac
     5101 ttctaagttt tggaattctc ccttaaatcc aacataagga atactaagaa cttccccacc
     5161 atcaacaggg tctgtaaaac gtacgtagcc ttctaggaaa tagccgtttg gcattacttt
     5221 ttttaacatg tcatggtatt tcgaaacatc tatatcaatt gtaatcgttt tagtttgccc
     5281 tggttcaatc cgtatcgttt tccctgtaaa tgtaccaagt tgttgaggag ccaatgtgac
     5341 aaagccatct ttaacttgat cagtatttaa ataggttgta tagtgtaaat cttttgcaac
     5401 tttattgata ttatgaacag taacatcaaa ggaaatttta tctttaatat ttcctaatgt
     5461 aacgctacca tagttgtttt ccccaccagt taggtataat cctgtctgaa ctgctgctgc
     5521 gacatcgata atacctgctc cttgttggcg tggtgaggtg taagcgcctg tatctttgtt
     5581 taggtgtgct ttagcagtac tcataagaag gtatttgaca gttctttcaa tgtcaccttt
     5641 tttaagttct ggatgttctt tcaataagta ttgtttaact agagcagtag caccagcgac
     5701 atggggagaa gccatacttg tcccactcat catatcatat tcattatcat tgatagcggc
     5761 gtaaatagag cctccaggag cagataagtc aggttttaat tgtccgtctg ctgttaatcc
     5821 ccagcttgaa aagtcagata ggacacctgc attagggttg gcttgtttat ttttgatatt
     5881 gttaaataca attttatagt tgtttttagc taaaacatct ccaaactctt tttgagtaaa
     5941 aatggcagga atagcgcttg cttcaggatc taaactcatt gttagattag cttctccagc
     6001 tttgttatta aagataatag ctcccacagc accgtgatta atagcgttga cgactttttt
     6061 tgtaaaagta atatctccac gttcaattaa ggcgatttta ccatttaaag ttttgtcctt
     6121 ataatcattg tcgtttcctt ttcctacgaa aacataatca tattgcttcc ctacttcaaa
     6181 ggtcttatca ctaactttag gatctgcata agcagctaat ccgttgttga gatttttgtt
     6241 gttttctaat ccgataatgt tgaagacttt attaaccagt gttgtattat tatatgatgc
     6301 tacagaaatg gcctcacgag ctgttgatgg actaccaact aaaccataat caggataaag
     6361 agcagaaggc tttgatgctc cactcccaaa tgtaccgtcg ttacctgctg ctataacaac
     6421 agaaacgcca gcgagtctag ccatctctaa agcttttata agtcggtcat cggcattaac
     6481 taaagagcca ttagctccac ctaaacttaa attaatgcta tcagcaccga gtttaacggc
     6541 atcttcaata gctttaacat aaagggctgg tccagttcct cttttttcat cagagaagac
     6601 tctcataaac attacttgtg cttcaggagc aacaccatag attaattcat ttgtatcttt
     6661 cttgctagga ttagctgttg cgatactggt tacgtgcata ccatgagaag tgcttttcac
     6721 ctcttttagc tctgtattga catcaacata gttgtgacca aagattactt tgttgttata
     6781 ccattttcca tagtttatac cagcttttgc tttagcagca ttcatttgct gttcgttttg
     6841 gtattttgct tttgtcgaat catttaattg gagagcatcg tgattggtat ctagtcctga
     6901 gtcaataatt gctacaacag ttccctctcc tttgtagcct gtgttccata ctttggggac
     6961 agtgataatt ttattactat caatgttttg aggggtttct ttagaaatag ctggtgtagg
     7021 tttgactgct aaattttgtt tggttaaagg agttttagaa acttcttgag tgctttcaac
     7081 cggttgattt gcggctgggg aaaatgtctc ggtggcttga gagtgaggct cagttactgg
     7141 ttgtgtttct gttggactat tattattagc ctttgtaaca agattgtctt gagctgttga
     7201 cgcaagggct gatgacgtgt tgttagtagg tgtctcattt gctatgctcg ttggactgtc
     7261 ttttacttgc tgttcaactg caatatcaga tggcttattt atgacagaat cagcagctac
     7321 atttgtgata ccgcctaaaa aaaatagggt tcccaaaagt acagatacgg cacctaactt
     7381 atatttccgg attgaaaaac gctgttttgt gttcataaaa atctcctttt cttgtaacat
     7441 aataattaca aaaactatta aaacataaaa aatcaaaatt ttctacaatt ttctgaattt
     7501 tcattacaaa ttattttgtg gtcaatttaa gaccgtacct aaagcatttt ttaaaaatca
     7561 ttctatgaaa ataataatat tgtccaacta tatggctaca atatcaaagg tttatagaat
     7621 aattacagga tatttattag tctaattgct ggcaaagcta aataatcatt cctcttcttt
     7681 cttagtaatc accgttgtgc tataataact gaatatactg aaaatttagg gggatatgat
     7741 ggaaaaagga tttaaagagg gagtgaaaga tgccttgccc acagctttag ggtatatctc
     7801 aataggcttg gcgtttggga tagttgcttc agcatcagat ttatctgcaa tagaagttgg
     7861 tcttatgagt gccttggtat atgggggatc tgctcagttt gctatgtgtg cgctattatt
     7921 agcaaaagca gacttgatga ctattacgat gacagtattt ttagttaatt tgagaaatat
     7981 gttaatgagt ttacacgcga ctactatctt caagtctgct cacctaatga atcaattagc
     8041 cataggaact ctaataacag atgagagcta cggcgtttta cttggagaag cattgcatca
     8101 caaagtcgta tcgcctagtt ggatgcatgg taataatgtg atgagttatt tgacatgggt
     8161 aatctcaacg ataataggaa ctttactggg gagtactatt cctaatccag aaatgtttgg
     8221 tttagacttt gcattagtcg ccatgtttat aggtctcttt gtatttcagt tgtttgggat
     8281 gttatctgat ggaaagcgac ttgttgtata tgttcttgct tctgtaggtt taagttactt
     8341 cttattatcc acttttttgt caggagcctt atcagtattg cttgcgactg ttgttggatg
     8401 tagtgtagga gttgttttag atgataaata gtaccgtttt tcttgctatt cttttaggtt
     8461 ttgttgtttc ttgggtgcca agagtcgctc cattcatatt tgtaaaatac aaagggctac
     8521 caaatagtct tgtgaaattt ttaaaatttc ttcctctttc tattttattt gccctaacat
     8581 tctctagctt tttttcagtt aaattaggac aattgccaac actaaaatgg ttagatttat
     8641 tggctggcat accaactcta gttgtcacta ttcgatatcg aaatttattt tacactgttc
     8701 ttactggtat cgttactatg gctttattga gattactatt ttaacgtgta ggaatggaag
     8761 attaataaaa acacaaaaaa cgcatctatt tctagatacg ttttttgtgt ttttgtctag
     8821 acaggaactt ttgatagccg atgtgtaata gtttgactaa ttgttaataa tatcaacaga
     8881 tttttctgta tcttgataat atgagttgat tgtaagattg ctttttaaag atatctacat
     8941 gctgctattt taaaatttat aaaaaggtcc atagtggttt atatagttta aaactatata
     9001 aattgctaaa aatataaaat attaaaagct attgatatta tagttttcat ggactaaaat
     9061 aattcataaa actaaaatgg ttagttatta gctataactt gatggaggaa attatggtaa
     9121 aagtttcaaa tttagggtat ccacgtcttg gtgaacagcg cgaatggaag caagcgatcg
     9181 aagctttctg ggcagggaat cttgaacaaa aagatttaga aaaacaacta aaacaattac
     9241 gtatcaatca tttaaagaaa caaaaagagg caggtattga ccttattcca gtgggggatt
     9301 tttcttgtta tgatcatgtt ttggatttgt catttcaatt caatgtaatc ccaaagcgtt
     9361 tcgatgagta tgagaggaat ttagaccttt attttgctat tgcaagaggt gacaaagata
     9421 atgtcgcatc atctatgaaa aagtggttta ataccaacta ccactacata gtcccagaat
     9481 gggaggttga gactaaacct cacttgcaga ataattactt acttgatctt tatctagaag
     9541 ctagggaagt agttggtgat aaagcaaagc cggttatcac tggtccaata acctatgttt
     9601 ccttatcatc aggaattgtc gactttgaag cgactgttca gcggttatta ccactttata
     9661 agcaggtctt tcaagatctg atagatgcag gcgccaccta tattcagatt gatgagccga
     9721 tatttgtaac tgatgaaggt gaacttttag tagatatagc taagtctgtc tatgattttt
     9781 ttgcaagaga agtaccacaa gcccacttca tctttcaaac ctactttgaa tcagcagtct
     9841 gtttagataa actctctaag ctgccagtaa cgggatttgg ccttgatttt atacatggta
     9901 gggcggaaaa tgtagctgct gttaagcaag gtctattccg cgaaaaagaa ttatttgcag
     9961 gaattgttaa tggtcgaaat atctgggcag taaatttaga agaaacgttg gctttattgg
    10021 aagagatagg tccctttgtt aaacgtttga cccttcaacc ttcttcaagt cttttacatg
    10081 taccggtgac gactaaatac gaaacacatt tagaccctgt gttaaagaat ggcttatcat
    10141 ttgctgatga aaaactaaag gaattagaac tattagcgag tgcttttgat ggtaataaaa
    10201 caaagggata tcacgaagct ttatctcgtt tttcagctct tcaagctgct gattttcgtc
    10261 atgtagcgtt ggaatcatta gcagaagtaa ggcttgaacg aagtccgtat aaattacgcc
    10321 aagctttgca agctgaaaaa ttacagttac cgattttacc aacaacaact attgggtcct
    10381 ttcctcaatc acctgaaatt aggaagaaac gccttgcttg gaaaagagaa aatctatctg
    10441 actcagatta taaagatttc ataaaaactg aaattagaag atggattgct attcaagaag
    10501 atcttgatct tgatgtgtta gtacatggcg aatttgagcg cgtggatatg gttgaatttt
    10561 ttggtcaaaa gttagctggt tttacgacaa ccaaattagg ctgggtacag tcttatggtt
    10621 caagggcggt caaaccacct atcatttatg gtgatatcaa acatattcaa cccttaagcc
    10681 ttgaagaaac ggtttatgcc caaagcttga ctaagaaacc tgttaaaggc atgttgacag
    10741 gtcctattac tataacgaac tggtcatttg agcgagatga tatcagccga tctgatcttt
    10801 ttaatcaaat tgctttggct ataaaagatg agattcaact tttggaacaa tcaggtattg
    10861 ctattataca agtggatgaa gcagcccttc gagaaggctt acccttacgc cagcaaaagc
    10921 aacaggctta cttagatgat gctgttgcgg cctttaaaat tgcaacttca tctgtgaaag
    10981 atgagacaca aattcataca catatgtgtt attcaaaatt tgacgaaatt attgattcta
    11041 tccgttcact agatgcagat gttatttcta ttgaaacgag tagaagtcat ggggacatca
    11101 ttgaaagttt tgaaacagca gtttatcctc taggaattgg tctgggtgtt tatgatattc
    11161 attctcctcg catacctact aaggaagaaa ttattgtcaa tattcaacga tcactaaaat
    11221 gtctatcaaa agagcaattt tgggtaaacc ctgactgtgg cttaaaaaca cgtcgtgaag
    11281 cagaaacaat tgctgccttg gaggttcttg tttcagctac caaagaggtt cgtcagcaat
    11341 tagataatta ataggttaat cagtaaagga ttgccatgag ggaggagtaa agaagatgtc
    11401 caagttttta gagaaattaa aaacagatat tctagtagct gacggtgcaa tgggaactct
    11461 cctttatact tatgggctag atacttgtca tgaatcctac aatgtaacac atccagaaaa
    11521 ggttctagcc attcatcaag cttatattga agcgggtgca gatgttattc agacgaatac
    11581 ctatggggct caacgtcatc gattaaagaa ctacggtttg gaagatcagg ttgtctctat
    11641 caatcaagca gcagttaata tagctcatca agcgactttg ggtaaagaaa catttatttt
    11701 aggtactgtt ggaggttttc gttctcaaag acaatgcgat ttaacattgg ataatattgt
    11761 agaagaaact ttggaacaag ttgaggcttt attggtgaca ggacagctag acggattatt
    11821 gtttgaaact tattatgata ttgaggagat aacgacagtt ttaaaaattg tgagggagat
    11881 gactgatcta ccaattatta ctaatatttc tcttcatgaa gcaggggtaa catcaaatgg
    11941 aaagcctatt gtagaggcgc tcagccaatt agttatgcta ggtgcagatg tgattggctt
    12001 aaattgtcac cttggaccct atcatatgat ccaatcattg aagcaagttc ctttatttgc
    12061 ccagtcttat ttgtcagttt atcctaatgc aggtcagtta tctttagatg gtgagaatag
    12121 tcaataccag tttagtcaga attcagagta ttttggaaaa tctgcagaac ttctcgtagc
    12181 agaaggagtt aggcttattg gtggttgttg tgggacaact ccagatcata ttagagctgt
    12241 gaaacgatct attagaggat tgaaacctat tgaacgaaag gttgttactc ctataatacc
    12301 tgtaaaagat tttgttaggc gtatcagacg aacagatact ctagtggata aggtaaaaaa
    12361 agaagtaaca attatcgcag aattagatcc tccaaaacat ttagatatcg ttcagtttca
    12421 aaaggctata cgagcgattg accaaaaagg tattgcagca attactttag cagataattc
    12481 actatctaat acacgtattt gtaatttaag tatagctagc ttgcttaaag acgaaattag
    12541 tacacccttt ttactacata tagcttgtcg tgaccataat ttaattggtt tacagtctag
    12601 actgttgggc atggaattat taggttttaa ccatatttta gccataacag gagatccgac
    12661 taaacttggt gatttcccag gagcgactag tgtttatgat gtgacttcct ttaaactcct
    12721 ttctctgatt aaacaactta atcaaggttt atcttatagt ggtgctagct tgagacgtcc
    12781 aacagatttt acagttgctg ctgcctttaa tcctaatgtg aaaaacctta ctcgaaccgt
    12841 aaaattgatt gagaaaaaag tagcttctgg tgctgattat tttatgacac aaccaatatt
    12901 tgatcactct gtgttaaggg aattagctga tcttactaaa acagttgaac aacccttttt
    12961 tatcggaatt atgccaataa caagttataa taacgctgtt tttctacaca atgaagttcc
    13021 tggtattaaa ctttctgagt catttttatc tgctcttgaa aaggtaaagg acgacaaaga
    13081 agcttgttta actttggcct taaacgagag taagtccttg attgatgaag ccttgaacca
    13141 ttttaatggt atctatttga ttactccctt tttacgctat gatctgactc ttgagttaat
    13201 tgactatata caaaaaaagc aagtaagaaa gtcctcttaa ctttcttact ttttagttgg
    13261 tcgtttatta ttataataaa agttacgtac caaaacagag gttaaactag atttttaagc
    13321 cactttctat actaagataa attagttaat ttaagttttc tttaagttta tagacttaga
    13381 ataaagcgtt attttgatgg gtagcataaa atcaaggcat aatatagatg tcagcgattt
    13441 ttttagacta acgaaataac gatgtaaagc ttaatagggg tgtatcgaat actcacttat
    13501 atagtaccaa aagcctatag cctgttggct agcttgaccg tattcaaatg ttgaataaat
    13561 cttggttgag aaagggaggt ataaaaatgg tatgtgtcga ttttattgat caaaaagttt
    13621 atagtgaagc agaaggtcta tttgtttccg atttagactt aggcattaaa ggtttaataa
    13681 gagcacgtta tcctttcgct ctggatagtg actttatttc gtatgggaat ttaagtcatt
    13741 attgtatgaa ttatcttgat gaaattgttc agcgagcgaa tcagaaaaat gagaagataa
    13801 aatataatgt gactaccttg atgaaagaag aagagaagcc cttcaacgtt gaagagcgtt
    13861 tgaacaagca agcaactatc ggtcaacgaa ttgctgatga tgttgcacgt tttggcggat
    13921 cttggacttt tattatcgtt tttgtatcca ttatggctat ttggatgttg gttaatatca
    13981 tgaagccttt tggtattcaa tttgatcctt acccatttat cctgttaaac ttagctttat
    14041 caacgattgc tgcgatccaa gcaccactta ttatgatgag tcaaaatcgt gctgctgatt
    14101 atgatcgttt gcaagctcga aatgacttta atgtcaataa aacctctgag ttagaaatta
    14161 gattattgca cgaaaaaatt gaccatatgg tacaacaaga tcagtttgaa ttgttagaga
    14221 ttcaaaagtt acagacagaa atgctagtat cattgggaaa tcagttagca caattgaaac
    14281 aactacaaaa ataatctttt agcagttatt taaaaatact gatacaatac taagaattta
    14341 aattaatact gcaacctcct ataatggaga gcagtatttt tactttatta ataacaaaaa
    14401 ccccttagat gatgtgtcta agggattttt gtatcggaat aatcgagttg gtgaattatt
    14461 aggctagtgt ctgtccgaaa gcgtctgctg ctaaaatagc ttgagcaaac attttgggat
    14521 cgaaaggggt atttttgtat tcattagtag tcattgcaat ttcaacaatt tttggaatat
    14581 ctttttctga taaagagacg tcagctagag ttgttggcaa acctagtgac ttattaaaac
    14641 gagctacttt ttcaagttct tctagttcgt tgaaataagc atgtaaaact aacacaccaa
    14701 acgcaactac aacgccatgt aagaattcac cttgtctttg aatagcggtt gtaccatagt
    14761 aaaatgcatg cgcgtgacaa gaattgtagt agaaatcagg ttgattaact aaattagaag
    14821 catagccagt tgagattaag atatcaagcg caatttcttc aactgcacgc gaagctaaat
    14881 tagcttcaac gtcttttaga ccttgttcac caaattgata aaaagcttcc tttgaagaca
    14941 gagcgactgc ttgtcctaac actgcagtat gtggtagttt attggtctta gcctctaagg
    15001 tagcacgttc tacttcaggg gcttttgaaa taccgtcccc aataccagcc caaaaatatt
    15061 tgcttggtgc ctgtgcaatg atccgtgtat tgataaaaat atgaagtgga gactcgggat
    15121 agccgtatct taagaaagaa tggtcatcat tatatacaac tgcaatggca gtacctgcgg
    15181 agcaattaga acaaatagtt gggatagtaa agctattttt acctaactct tttgctacca
    15241 ttttagctgt gtctaaagct tttccaccgc caaaacctaa gatagcatca gcagcttgaa
    15301 cttgtgggtt ggctactaac ttgtcaatgt tagattgtgt tgaatccgtt ccatagacaa
    15361 agcttcctgt gactttaata tttgcttcct ctaaaataga gcgaacctcg gcttctgagc
    15421 tgtctagggc acgtttgcca cccacgaaag caatgctttg aatacggtaa gttgccaaaa
    15481 cgctaggaat ttctttgaag caatcttctc cgtagctata atttgccatt acatttgttg
    15541 tcattcagtg tcctcctcta ttccaaaggt cgattagaga cagctatttt ctaaaagtga
    15601 atagtttttt gtctcttgac caagtgatta tcatattata gcagaatatt cagaaaattg
    15661 gaacatgaaa aataatttaa ttttcaagaa taagaataga aaaggtaaag cgagatatac
    15721 gagtaaatac tgtcagtata ataattataa gctcttgaga gggtctgtat tggatgcaac
    15781 agggagttct tttaggtatt gatcaagatc gcgttggtgt tttagcacct ttattttttg
    15841 agggtagtcc tcaacaactt gtttatagtt tgatttttgt ttgtcagagc gaccgtcttt
    15901 aagaatccaa ctgataaatt caaaatctaa tttttccggg catccattgg ccatatcagg
    15961 tctagtctta ccacgaaact tacagtaacg tttgaatgct ctccacaagc agttgaagcg
    16021 attaaagtca aaaaaaataa tctcatctgc atctgctaga cgttcttgat atagcagttt
    16081 tttgtagtta ccttctataa tccatgttct tttttctaac atgctgctga catcgtcaat
    16141 catgtcatca tattttctct cttcccaatt tggggcgaag tgaatactat caaggtgtaa
    16201 tacattgcaa ttatagtaat ttcccaattt tctcgctaaa gtggacttac ctgacccact
    16261 atatccgatt attgctattt tcatatacta cctccgttaa attgtttact tgaagttaat
    16321 gctgtactta ggatttactt attttataag ggttattcac ttt
//
LOCUS       DATRUK010000046        16183 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.046, whole genome shotgun sequence.
ACCESSION   DATRUK010000046 DATRUK010000000
VERSION     DATRUK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16183
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.046"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(27..97)
                     /locus_tag="VBE34_001402"
     tRNA            complement(27..97)
                     /locus_tag="VBE34_001402"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63..65),aa:Cys,seq:gca)
     gene            complement(231..1763)
                     /gene="ahpF"
                     /locus_tag="VBE34_001403"
     CDS             complement(231..1763)
                     /gene="ahpF"
                     /locus_tag="VBE34_001403"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEO7264116.1"
                     /translation="MVLDKEIKAQLAQYLDLLESDIVLQTDLGDNDNSQKVKDFLDEI
                     VAMSDRISLESTHLKRQPSFGIAKKGQESRVIFSGLPMGHEFTSFILALLQVSGRAPK
                     VDEDIIKRIKGIEKTINLETYVSLTCHNCPDVVQAFNIMAVLNPNITHTMIEGGMYQD
                     EVKSKGIMSVPTVYKDQEEFTSGRATIEQLLEQLDGPLDAEAFADKGVYDVLVIGGGP
                     AGNSAAIYAARKGLKTGILAETFGGQVIETVGIENMIGTLYTEGPKLMAQIEEHTKSY
                     DIDIIKSQLATGIEKKELVEVTLANGAILQAKTAILALGAKWRNINVPGEEEFRNKGV
                     TYCPHCDGPLFEGKNVAVIGGGNSGMEAALDLAGVTKHVTVLEFLPELKADQVLQERA
                     AKTDNLTILKNVATKDIVGEDHVTGLNYTDRDTNEEKHIDLEGVFVQIGLVPSTSWLK
                     DSGIELNERQEIVVDKFGSTNIPGIFAAGDCTDAAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(1781..2341)
                     /gene="ahpC"
                     /locus_tag="VBE34_001404"
     CDS             complement(1781..2341)
                     /gene="ahpC"
                     /locus_tag="VBE34_001404"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEO7264117.1"
                     /translation="MSLVGKEIIEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLDVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQGFDVLGQDGLAQRGTFIIDPDGVIQMMEINADGIGRDASTLIDKVRAAQYIRQH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            2618..3388
                     /gene="rpsB"
                     /locus_tag="VBE34_001405"
     CDS             2618..3388
                     /gene="rpsB"
                     /locus_tag="VBE34_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEO7264118.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVAEEAKRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFELLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDVIIPANDDAIRAVKLITSKLA
                     DAVIEGRQGEDADVDFAQEAQADSIEEIVEVVEGSNND"
     gene            3482..4522
                     /locus_tag="VBE34_001406"
     CDS             3482..4522
                     /locus_tag="VBE34_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEO7264119.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDLDKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVDGNVAAVIEVNAETDFVAKNDQFVTLVNETAKVIAE
                     GRPSNNEEALALTMPSGETLEQAFVTATATIGEKISFRRFALVEKTDEQHFGAYQHNG
                     GRIGVITVVEGGDDALAKQVSMHVAAMKPTVLSYTELDAQFVHDELAQLNHKIEQDNE
                     SRAMVNKPALPFLKYGSKAQLTDEVIAQAEEDIKAELAAEGKPEKIWDKIVPGKMDRF
                     MLDNTKVDQEYTLLAQVYIMDDSKTVEAYLESVNAKAVAFVRFEVGEGIEKASNDFEA
                     EVAATMAAALEK"
     gene            complement(4681..5142)
                     /locus_tag="VBE34_001407"
     CDS             complement(4681..5142)
                     /locus_tag="VBE34_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HEO7264120.1"
                     /translation="MNYIITFLISMIPLVELRGAVPFAIANGIPLWEALAIGVVGNML
                     PVPIIFFFARKVLEWGADKPYTGKFFTWCLKKGHSGGQKLEKVAGEKGLFIALLLFVG
                     IPLPGTGAWTGTLAASLLDWEFKHSVIAVMLGVILAGCIMGTLSIIGFNLF"
     gene            5315..5779
                     /locus_tag="VBE34_001408"
     CDS             5315..5779
                     /locus_tag="VBE34_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEO7264121.1"
                     /translation="MAIKNTSDNIEEYIKSLLEQSGIAEIKRSNLADTFQVVPSQINY
                     VIKTRFTESRGYVVESKRGGGGYIRIAKVHFSDQHQLFGNMLSTIGERISEQVFDDLI
                     QLLFDEEIITEREGNLILATSGDDVLGEQASVIRARMLRKLLQRLDRKGFHL"
     gene            5776..8223
                     /locus_tag="VBE34_001409"
     CDS             5776..8223
                     /locus_tag="VBE34_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO7264122.1"
                     /translation="MSHYSIKLQEVFRLAQFQAARYESHYLESWHLLLAMVLVHDSVA
                     GLTFAEYESEVAIEEYEAATILALGRAPKEEITNYQFLEQSPALKKILKLAENISIVV
                     GAEDVGTEHVLLAMLVNKDLLATRILELVGFRGQDDGESVRMVDLRKALERHAGFTKD
                     DIKAIYELRNPKKAKSGASFSDMMKPPSTAGDLADFTRDLSQMAVDGEIEPVIGRDKE
                     ISRMVQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGNIPYELRDMRVLELDMMS
                     VVAGTRFRGDFEERMNQIIADIEEDGHIILFIDELHTIMGSGSGIDSTLDAANILKPA
                     LARGTLRTIGATTQEEYQKHIEKDAALSRRFAKVLVEEPNLEDAYEILLGLKPAYEAF
                     HNVTISDEAVMTAVKVAHRYLTSKNLPDSAIDLLDEASATVQMMIKKNAPSLLTEVDQ
                     AILDDDMKSASKALKASHKDKKHKPIAVTEDHIMATLSRLSGIPVEKLTQADSKKYLN
                     LEKELHKRVIGQDDAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKALA
                     EVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTEKVRNKPYSVLLFD
                     EVEKAHPDIFNVLLQVLDDGVLTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFGA
                     KDISHDYTAMQKRIMEELKKAYRPEFINRIDEKVVFHSLSQDNMREVVKIMVKPLILA
                     LKDKGMDLKFQPSALKHLAEDGYDIEMGARPLRRTIQTQVEDHLSELLLANQVKEGQV
                     IKIGVSKGKLKFDIAKS"
     gene            complement(8443..8934)
                     /locus_tag="VBE34_001410"
     CDS             complement(8443..8934)
                     /locus_tag="VBE34_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEO7264123.1"
                     /translation="MIIRPVLPSDAKELLAIYAPYVTDTTITFEYDIPSISEFTNRIK
                     NISKSFPYLVAEENGKILGYAYASTYYARASYDWTCELSIYLDKDVRSKKIGSQLYDT
                     LEKELVNQGYVNLLACISLPNDISIAFHKKRGFNQVAHFPKIGFKFEQWHDIVWLQKR
                     LKS"
     gene            complement(8948..9589)
                     /locus_tag="VBE34_001411"
     CDS             complement(8948..9589)
                     /locus_tag="VBE34_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEO7264124.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGQHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIFFLNKRFQSIKEAYKANNNVLDRSIFEDELFLTLNFKNGNVTKTELD
                     IYKELLANMLEELEGMPKKRPDLLVYIDVSFDKMLERIDKRGRSFEQVDSNPELYDYY
                     KQVHSEYPEWYENYDVSPKIRIDGNKLDFVKNPEDLQHVLDTIDSELQKLDLL"
     gene            complement(9710..10687)
                     /gene="dusB"
                     /locus_tag="VBE34_001412"
     CDS             complement(9710..10687)
                     /gene="dusB"
                     /locus_tag="VBE34_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEO7264125.1"
                     /translation="MTQLNSSFMIGKVEIPHRTVLAPMAGITNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQNNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPEKIYHIVKEVTSVLDIPLTVKMRTGWSDSSNAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLGKVAKAVTSIPFIANGDIRTVHDAK
                     FMIEEIGADAIMVGRGARSNPYIFTQINHFFETGEILPDLPFEKMLDVAEDHLTRLVN
                     LKGETIAVREFRGLAPHYLRGKSGAAKIRGAVSRAETLAEVQELFAGLR"
     gene            complement(10671..11546)
                     /gene="hslO"
                     /locus_tag="VBE34_001413"
     CDS             complement(10671..11546)
                     /gene="hslO"
                     /locus_tag="VBE34_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEO7264126.1"
                     /translation="MDKIIKSISTSGSFRAYVLDCTETVRTAQEKHQTLSSSTVALGR
                     TLIANQILAANQKGNSKVTVKVIGDSSFGHIISVADTKGNVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVVITDYGTGQPYTSTTPLITGEIGEDFAYYLTESEQTPSAVGLNV
                     LLDDEDKVKVAGGFMLQVLPGASDEEISRYEKRIQEMPSISSLLESENHIESLLSAIY
                     GEDDYKRLSEDSLAFYCDCSKERFEAALLTLGTKELQAMKDEDKGVEITCQFCNQTYY
                     FTEEDLEKIINDSIK"
     gene            complement(11686..12942)
                     /locus_tag="VBE34_001414"
     CDS             complement(11686..12942)
                     /locus_tag="VBE34_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEO7264127.1"
                     /translation="MSDTFNFDIDQIADNAITKTDKTTEIISNQTTSQTGQIAFFEKL
                     TPAQKSAISEKTPALVDTFVGDQNALLDFGQSAVEGVNTTVNHILSEQKKIQIPQVDD
                     LLKNANRELNGFIAKYKDATPAELEKKPNLIQKLFKQSKTSLQEFYFDSQNIEQKMDM
                     MAANVVKQEDTLARNIVSAEMLIEDNTKSIENLVGVIAFIESSQAEAANRASHLQQEI
                     LALDSQTSEYQIKSNQLARMTEVINTLEQQHTEYVSRLYVAWATTPQMRNLVKVSSDM
                     RQKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGVTADAIVNANNAALQMLAETSKEAIP
                     MLEKTAQSPTVSIKSVTALAESLVAQNNGIIAAIDKGRKERAQLESAVIKSAETINDS
                     VKIRDKKIVEALLNEGKSTQEKVDES"
     gene            complement(12939..13757)
                     /locus_tag="VBE34_001415"
     CDS             complement(12939..13757)
                     /locus_tag="VBE34_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264128.1"
                     /translation="MVKKRYSKNSHNLITLLGIVVLASLISDFWSEVIATLLIIGGGY
                     CAYYVYDKKRLKCFTSNQRIEALKSDIKETDQDIRHLEILKKDNRSKEYIKLAHQILP
                     QLDLIRNEANQLQKAIEPNIYKRITKKANTFSNEINEQLIKLHASPELEPISDQEDEM
                     IRIAPELKPFYHNIQDDHYAILKKIEEADNKAELAAIHQANMKRFTDVLAGYIRIKQS
                     PKNFNNAKERLEQALQAIKKFNLDLDETLRQLNESDMKDFDVSLRMMQDERNNK"
     gene            complement(13881..16133)
                     /gene="gshAB"
                     /locus_tag="VBE34_001416"
     CDS             complement(13881..16133)
                     /gene="gshAB"
                     /locus_tag="VBE34_001416"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEO7264129.1"
                     /translation="MIIDQLLQRSHSHLPILQATFGLERESLRIHQPTQRVAQTPHPK
                     TLGSRNYHPYIQTDYSEPQLELITPIAKDSQEAIRFLKAISDVAGRSINHDEYLWPLS
                     MPPKVREEDIQIAQLEDAFEYDYRKYLEKTYGKLIQSISGIHYNLGLGQELLTSLFEL
                     SQADNAIDFQNQLYMKLSQNFLRYRWLLTYLYGASPVAEEDFLNQKLNNPVRSLRNSH
                     LGYVNHKDIRISYTSLKDYVNDLENAVKSGQLIAEKEFYSPVRLRGSKSCRNYLEKGI
                     TYLEFRTFDLNPFSPIGITQETVDTVHLFLLALLWIDSSSHIDQDIKEANRLNDLIAL
                     SHPLEKLPNQAPVSDLVDAMQSVIQHFNLPPYYQDLLESVKRQIQSPKLTVAGQLLEM
                     IEGLSLETFGQRQGQIYHDYAWEAPYALKGYETMELSTQLLLFDVIQKGVNFEVLDEQ
                     DQFLKLWHNSHIEYVKNGNMTSKDNYIVPLAMANKVVTKKILDEKHFPTPFGDEFTDR
                     KEALNYFSQIQDKPIVVKPKSTNFGLGISIFKTSANLASYEKAIDIAFAEDSAILVEE
                     YIEGTEYRFFVLEGDCIAVLLRVAANVVGDGIHTISQLVKLKNQNPLRGYDHRSPLEV
                     IELGEVEQLMLEQQGYTVNSIPPEGTKIELRRNSNISTGGDSIDVTNTMDPTYKQLAA
                     EMAEAMGAWVCGVDLIIPNATQAYSKDKKNATCIELNFNPLMYMHTYCQEGPGQSITP
                     RILAKLFPEL"
BASE COUNT         5026 a         2859 c         3060 g         5238 t
ORIGIN      
        1 tctttggata aaatctttta tatttaaggc ggtagacgga tttgaaccga cgatcaagct
       61 tttgcagagc cgtgccttac cacttggcta taccgccata acagataata ttctatcgaa
      121 aaaaaaaaga ttcgtcaagt ctctttcgta aaatagccaa ttttagccct taaacttcct
      181 catattacca aaaaaagaga ttgatacaat gggtatcaat ctcttttaga ttattgtctg
      241 attaaataat caaatgcgcc aatggcagct gtggcaccag accccatgga aataatgatt
      301 tgcttgtagg cagcatctgt acagtcacct gcagcaaaaa taccagggat atttgttgag
      361 ccaaatttat caacgacaat ttcttgacgt tcattgagtt caatgccgct atccttgagc
      421 caagaagtac ttggtacaag accaatttgt acaaaaacac cttctaaatc aatgtgtttt
      481 tcttcgtttg tatcacgatc ggtatagttt aaaccagtga catgatcttc accaacgata
      541 tctttagtag caacattctt aagaatagtt aaattgtcag ttttagctgc tcgttcttgc
      601 agtacttggt ctgctttgag ctcaggtaag aattctagga ctgtaacatg tttagtgaca
      661 ccggctagat ctaaagcagc ttccatacct gaattaccac cacctataac agcaacattt
      721 tttccttcga aaagagggcc atcacaatgt ggacagtaag taacaccttt attgcggaat
      781 tcttcctcgc caggaacgtt gatatttcgc cacttagcac ccagagctag gatagcagtt
      841 ttagcttgta aaatagctcc gttagccagt gttacctcaa ctaattcttt tttctcaatg
      901 ccagttgcta attgactctt gataatatca atatcgtaag atttggtatg ctcttcaatt
      961 tgagccatta atttagggcc ttcggtgtac agtgtaccaa tcatgttctc aataccaact
     1021 gtttcgataa cttgtccacc aaaagtttct gctaaaatac ctgtttttaa ccctttacga
     1081 gcggcataaa ttgcagcact gttacctgca ggaccaccac caataactag aacatcatag
     1141 acacctttgt cagcaaatgc ttcagcatca agaggtccat ctagttgttc taaaagctgt
     1201 tcaattgtag cacggccaga agtaaattct tcttggtcct tataaacagt aggaacagac
     1261 atgatacctt ttgatttgac ttcgtcttga tacatgcctc cttcaatcat agtatgagtg
     1321 atatttggat tcaaaacagc cattatatta aaggcttgaa cgacatctgg acaattatgg
     1381 caagttaatg aaacataagt ttctagattg atagttttct caatcccctt aattcgtttg
     1441 ataatatctt catcaacttt tggtgcgcga ccagagactt gcaacaaagc taaaatgaaa
     1501 gatgtgaatt catgccccat tggcaagcct gaaaaaataa cgcgactttc ttgccctttt
     1561 tttgcaatcc caaaactagg ttgacgtttt aaatgtgttg attcaaggct gatacgatca
     1621 gacatagcga caatttcatc tagaaaatcc tttacttttt gagaattatc gttatctcct
     1681 aaatcagttt gtaatacaat atcagattct agcaaatcta aatattgcgc taattgagcc
     1741 ttgatttctt tatctaagac catggattac ctcctggtta ttaaatttta cctacaaggt
     1801 ctaaactagg agttaaagtt tcagctcctt ctttccattt agctggacaa acttcacctg
     1861 gatgttggcg gatgtattga gcagcacgga ctttatcaat gagagtactt gcatcacgtc
     1921 cgatgccatc agcattaatt tccatcattt ggatgacacc gtctggatca atgataaatg
     1981 taccacgttg tgctagacca tcttgtccaa ggacatcaaa accttgtgaa ataaggtgtg
     2041 aagggtcacc aatcatagga tatgtgatag taccaacgac atctgagtca tcatgccatg
     2101 ctttatgaac aaaatgagta tcagtagaga cagaataaac ttctacatca agagatttaa
     2161 gtgtttcgta ttgctcttga aggtcaccaa gttcagttgg gcaaacaaat gaaaagtctg
     2221 ctgggtagaa acaaaaaact gcccattttc ctttaacgtc ttcatttgta acagtgatga
     2281 attttccatc gtgataagct tgagctgaaa attcaataat ttcttttccg actaatgaca
     2341 taataatgtc ctcctttatt ttttaatagc ctaattataa acgaaatact tttaaatctc
     2401 caataaaaac gtttacattt ttctaagaaa agtatgattt ttctaataga gtggaagatt
     2461 ttatagcttg tcaaatctaa ctttttttga taaactacta tagttgctta aagtgacaaa
     2521 tactcatctt agatcaattg tggtcttgtt gccgaaaggt taggctgcaa gtcgaagtct
     2581 gagagaggaa aaaacatttt taaaggagaa cttactcatg gcagtaattt caatgaaaca
     2641 acttcttgag gctggtgttc actttggtca ccaaactcgt cgctggaacc ctaagatggc
     2701 taagtacatc ttcacagaac gtaatggtat ccacgttatc gaccttcaac aaactgtaaa
     2761 attagctgac caagcttacg aatttgttcg tgatgctgca gcaaacgatg cagttatctt
     2821 gtttgttggt actaagaaac aagcagctga ggctgttgct gaagaagcta aacgtgctgg
     2881 tcaatacttc attaaccacc gttggttagg tggaactctt actaactggg gaactatcca
     2941 aaaacgtatt gctcgtttga aagaaatcaa acgtatggaa gaagaaggaa cttttgaact
     3001 tcttcctaag aaagaagttg cattgttaaa caaacaacgt gcacgtcttg aaaaattctt
     3061 gggtggtatc gaagatatgc cacgcatccc agatgtaatg tatgttgttg acccacataa
     3121 agagcaaatc gctgttaaag aagctaaaaa acttggtatt ccagttgtag ctatggttga
     3181 cactaacgct gatccagatg atatcgatgt tatcatccca gcaaacgatg acgctattcg
     3241 cgccgttaaa ttaattactt ctaaattagc tgatgcagtt attgaaggtc gtcaaggtga
     3301 agatgctgac gttgacttcg ctcaagaagc acaagcagat tcaattgaag aaatcgttga
     3361 agtagttgaa ggatcaaaca acgactaata tttaatttat tgtaaatcct aaaacggggc
     3421 agatgcaaac cgctctgtct cgttttttta atcattattt gaaattaaga ggagaacgaa
     3481 aatggcagaa atcacagcta aacttgtaaa agaattgcgt gaaaaatcag gtgcaggcgt
     3541 tatggacgct aaaaaagcat tagtagaaac tgatggtgac cttgataaag cgattgaatt
     3601 acttcgcgaa aaaggtatgg ctaaagcagc taaaaaagca gaccgtgttg ctgctgaagg
     3661 tttaacaggt gtttatgttg atggtaacgt tgcagcagtt attgaagtta acgctgagac
     3721 tgactttgtt gctaaaaacg accaatttgt aacattagtt aatgaaactg ctaaagttat
     3781 tgcagaagga cgtccttcaa acaatgaaga agcacttgct ttaacaatgc cttcaggtga
     3841 aactttagaa caagcatttg taactgcaac tgctacaatt ggtgaaaaaa tttcattccg
     3901 tcgcttcgct ttagttgaaa aaactgatga gcaacatttt ggtgcatacc aacataatgg
     3961 tggacgtatc ggagtaatta ctgttgttga aggtggcgac gatgcacttg ctaaacaagt
     4021 atcaatgcac gttgcagcta tgaagccaac tgttctttca tatactgaac ttgatgcaca
     4081 atttgttcat gatgaattag cacagttgaa ccacaaaatt gagcaagaca acgagagtcg
     4141 tgctatggtt aacaaaccag ctcttccatt ccttaaatat ggatcaaaag ctcaattgac
     4201 tgatgaagtt attgcccaag cagaagaaga catcaaagca gaattagctg cagaaggtaa
     4261 accagaaaaa atctgggata aaatcgttcc aggtaaaatg gatcgtttca tgcttgacaa
     4321 cactaaagtt gaccaagagt acacactcct tgctcaagtt tacatcatgg atgatagcaa
     4381 aacagttgaa gcttatcttg agtcagtaaa tgctaaagca gttgctttcg tacgttttga
     4441 agttggagaa ggtattgaga aagcttctaa cgactttgaa gcagaagttg cagctacaat
     4501 ggcagcagca cttgaaaaat aattaacact cgctttatat tataatttgg cagcaaaatg
     4561 ttgtcagtaa atatatttta aaataagaca cttggcttaa gtcaagtgtc ttattttgct
     4621 atcgaattgg tggattggca aatattaaaa aggagagaca gtcatctcac cttaactttt
     4681 ctagaatagg ttgaagccga taatggataa tgttcccatg atgcatccag ctagtataac
     4741 acctagcata acagcaataa cactatgttt aaattcccaa tctagaaggc tagctgcaag
     4801 tgtgccagtc catgctcctg ttccgggtaa gggaatgcct acaaacaata gcaaggcaat
     4861 aaacaatcct ttttctccag ctactttttc aagtttttga ccaccgctat gtcctttttt
     4921 aaggcaccat gtgaaaaact taccagtata aggtttatct gctccccatt ctagaacttt
     4981 gcgagcaaag aagaagatga taggtacagg tagcatgttt ccaacaacac cgatagctag
     5041 cgcttcccac aaaggtattc cattagcgat tgcaaatggc acagctcctc gtagttccac
     5101 taagggaatc attgaaatca aaaatgtaat aatgtaattc attaaataaa acctttctat
     5161 ggtattattc cattttatct tatcttgcta gtaatgacaa ctaaaatccg cggtcattct
     5221 agttttacta tccttagata gatttcattt ccgtatcttt taaaaatttg atataataat
     5281 agtcagttat agtcaaagag gaaggagtga atttgtggcc ataaaaaata cttccgataa
     5341 tattgaagaa tatataaaat cattattaga acaatcagga attgctgaaa taaagcgttc
     5401 caatttagct gatacttttc aagttgttcc tagtcagatt aattatgtca ttaagacacg
     5461 gtttactgaa agccgtggtt atgtggttga gagtaaacga ggtggtggag gttacatccg
     5521 cattgctaag gttcattttt ctgaccaaca ccaattattt ggaaatatgt tatcaacgat
     5581 tggcgagcgt atcagtgaac aagtctttga tgatttgatt caattattat tcgatgaaga
     5641 gataattaca gaacgtgaag gaaatttgat tctggcgaca tcaggtgatg acgtcttagg
     5701 agagcaagcc tctgttatta gagctcgtat gctccgtaaa ttattacaac gattagatag
     5761 aaaaggattc catttatgag tcattattcc ataaaattac aagaagtatt cagattggcg
     5821 caatttcaag cagcacgcta tgaaagtcac tatttagaaa gctggcatct tcttttagca
     5881 atggttttag ttcatgattc agtagctggt ttgacatttg ctgaatatga gtctgaagtt
     5941 gcaattgaag agtacgaagc agcaacaatt ttagctttag gtagagcacc gaaagaagag
     6001 attaccaatt atcaattttt agagcaatca ccagctttga aaaagatctt aaagttagca
     6061 gaaaatatta gtatcgtagt aggagctgaa gatgtgggta ctgaacatgt cttgcttgct
     6121 atgttggtta ataaagattt attagccaca cgtatcttag aattagttgg ctttagaggt
     6181 caagacgatg gtgaatcagt tcgtatggtt gatttacgta aggctctaga acgtcatgcc
     6241 ggttttacaa aagatgatat caaagcaatt tatgaattac gtaatcctaa aaaagcaaag
     6301 tcgggtgctt ctttttctga tatgatgaaa ccaccaagta ctgctggtga tttagctgat
     6361 ttcacacgtg atttatcaca gatggcggtt gatggggaaa ttgaacctgt tattgggcgt
     6421 gataaagaaa tttcacgtat ggtccaagtc ttaagtcgta aaacaaagaa taatcctgtc
     6481 ttagttggag atgcgggtgt cggtaaaaca gctttggcgt acggtctagc acaacgtatt
     6541 gctaatggaa atattcctta tgagcttaga gatatgcgtg ttctagagtt agatatgatg
     6601 agcgttgttg ctggaactcg ttttcgtggt gattttgagg aacgtatgaa tcaaatcatt
     6661 gcagatattg aagaagacgg tcatattatt cttttcattg atgaactgca tactattatg
     6721 ggttctggct ctggtattga tagcacactt gatgctgcta atattttaaa accagctttg
     6781 gctcgaggca ctttacgcac tattggagca acaactcaag aggaatatca aaagcatatt
     6841 gaaaaagatg ctgctttatc tcgtcgcttt gcaaaagttt tagtagaaga accaaatctt
     6901 gaagacgcat atgaaattct acttggattg aagccagcgt atgaagcctt tcataatgtt
     6961 accatttcag atgaagcagt tatgactgct gtaaaagtgg ctcatcgtta tttgacaagt
     7021 aagaatttac cagactcagc tatagactta cttgatgaag cttctgccac tgtgcaaatg
     7081 atgattaaga aaaatgctcc atcgctttta acagaagttg accaagctat tttagatgat
     7141 gatatgaaat ctgcttctaa agctctaaaa gcaagtcata aggataaaaa gcataagcct
     7201 attgccgtaa ccgaagatca tattatggca actctcagcc gtctatctgg tattcctgtt
     7261 gaaaaattga cacaagctga tagtaaaaaa tacctcaatt tagaaaaaga attgcataaa
     7321 cgtgttatcg gtcaagatga tgcagttact gccattagtc gtgctatccg tcgcaatcaa
     7381 tctggcattc gtactggtaa gcgtcctatt ggttccttta tgttcttggg accaactggt
     7441 gttggtaaga cagagttagc aaaagccttg gcagaagtgt tgtttgatga tgaatcagcc
     7501 ctaattcgtt ttgatatgtc ggaatatatg gaaaaattcg cagcttctcg cctgaatggg
     7561 gcacctccag gttatgttgg ttatgatgaa ggaggagagc tgactgaaaa agtacgcaat
     7621 aaaccatatt cagttttact ttttgatgaa gttgaaaaag ctcatccaga tatttttaat
     7681 gtattattac aagtgcttga tgatggcgtt ttaacagata gtcgaggacg aaaagttgac
     7741 ttttcaaata ctataatcat tatgacttct aatttaggag caactgcatt acgtgacgat
     7801 aaaacagttg gttttggagc taaggatatc agccatgatt atactgctat gcaaaaacgt
     7861 attatggagg agcttaaaaa agcttatcga ccagagttta tcaatcgtat tgatgagaaa
     7921 gttgtattcc atagtctcag tcaagataat atgcgtgaag ttgtgaaaat tatggttaaa
     7981 cctttaattt tagcgcttaa agataaaggt atggatctga aattccagcc ttcagccttg
     8041 aaacacttgg cagaggatgg ctatgatata gaaatgggtg ctcgtccact gcgtagaact
     8101 attcaaacgc aagtagagga tcatttatca gaactactat tggcaaatca agtgaaagag
     8161 gggcaagtaa ttaagattgg cgtatccaaa ggcaaattga aatttgatat tgcaaaatca
     8221 taaaacattc cagtaccgat gggtactgga attttaatat aaaaagaaaa tgtccaaaat
     8281 attttggaca ttttcttata gatttatgaa aagtaaaagg atctatatct aacaattgca
     8341 cttgtagata taggtacgac tgaaagtcgt tgaaaaattt taggataaat atattataat
     8401 atcctaatct tttgctaact taaaataaat ctaaagatta ccttaagatt tcaatcgctt
     8461 ttgtaaccat acaatgtcat gccattgttc aaatttaaac ccgatttttg ggaaatgtgc
     8521 gacttgatta aatcctcttt ttttgtggaa tgcaatgctt atatcatttg gtaaactgat
     8581 acaagcgagt agattaacat acccttggtt aactaattct ttctccaatg tatcatatag
     8641 ttggctacca atttttttgc tacgtacatc tttatcaagg taaatagaaa gctcgcaagt
     8701 ccagtcatag gaagctctag cgtagtaagt tgaagcatat gcataaccta atattttccc
     8761 attttcttca gcaactaaat atgggaaaga tttgctgata tttttgatac gattagtgaa
     8821 ttccgatata ctaggaatat cgtattcaaa cgttattgta gtgtcagtaa cgtagggagc
     8881 atagatagca agtaattctt tagcatctga gggtaggaca ggtcttataa tcatagatat
     8941 cacctaatta tagtaaatca agtttttgca attcggaatc aatagtgtca aggacgtgtt
     9001 gaagatcctc tggatttttg acgaaatcaa gtttgttacc atcgatccga attttaggtg
     9061 aaacgtcgta attttcatac cattctggat attcagagtg gacttgtttg tagtagtcgt
     9121 ataattctgg gttagaatct acttgctcaa agctgcgtcc tcgtttgtca atacgttcta
     9181 gcattttatc aaatgaaaca tcaatgtaga ctagcaaatc aggtctcttc ttaggcatac
     9241 cttctaattc ttccaacata tttgcaagaa gttctttata gatatctagc tctgttttag
     9301 tcacatttcc attcttaaaa tttagagtga ggaatagttc atcttcaaaa atagaacgat
     9361 ccaaaacatt gttgttagcc ttataggctt cttttataga ttggaatcgt ttatttaaaa
     9421 agaatatttg tagtaaaaaa gcatattttt tgggatcttg atagtaaaga tctaaaactg
     9481 ggttattatc aaccgcctca tagaaaacat ctgtccctag gtgttgtcct agagcagctg
     9541 caagggagct tttacctgct ccaatggttc cagctaaaac aatcaacata attctcctta
     9601 attctgttat actaaaataa tttgttatac ttttgagttt tcattacaca atgatttatg
     9661 ctaaatttgt acagagatag gaaaaaacag tcaaaggact gtttttgatt tagcgtaaac
     9721 ctgcaaaaag ttcttgaact tctgctagtg tttctgcacg tgaaacagct cctctaattt
     9781 ttgctgcgcc tgattttcca cgtagataat gaggtgctaa ccctcgaaat tcacgaacgg
     9841 caatagtttc accttttaaa ttaactagtc gagtgagatg atcctcggca acatctaaca
     9901 tcttctcaaa tggtaggtcg ggaaggattt cgcctgtttc aaagaaatga ttaatttgtg
     9961 taaagatgta aggattgcta cgagcaccac gtccaaccat tatagcgtca gctccaatct
    10021 cttcaatcat aaatttggca tcgtgaacag tacgaatgtc accattggca ataaaaggaa
    10081 tactagtaac agctttagcg accttgccaa gtgtttcatg gtcacacgtg ccagtataca
    10141 tttgttcacg tgttcgaccg tgcatagcaa gtgcagatac accagcagat tcggcagcga
    10201 gtgcattttc tacagcatta gatgagtcag accatcctgt acgcattttt actgttaatg
    10261 gaatgtcaag tacagacgtt acctctttaa caatatgata aattttttca gggtcacgta
    10321 accatttagc tccagcttcg tttttaacaa ctttgttaac tggacatccc atattgatgt
    10381 ctacgatatc tgctttcgta ttattttgga taaagtcagc tgcacgtttt aagccttctg
    10441 catcgccacc aaatagttga attgacatag gatgttcatt ttcatcgata tggagcatat
    10501 gtaaggtctt ttcgttgtta taaagtaacc ctttctcaga aatcatttcc ataacaacga
    10561 gaccagcacc aaattcttta gcgatagtac gaaaagcaga attagtaata cctgccatcg
    10621 gtgctagtac ggtacgatga ggaatttcaa ccttcccaat cataaatgaa ctatttaatt
    10681 gagtcattaa ttatcttctc caaatcttct tctgtgaaat agtaagtctg gttgcaaaat
    10741 tgacaagtaa tttcaacacc tttatcttca tccttcatgg cttgcaattc tttagttcct
    10801 aaagtcaata gagcggcttc aaaacgttct tttgaacagt cacaatagaa ggctaaagag
    10861 tcttccgata agcgcttgta gtcgtcttct ccgtaaatag ctgaaaggag agattctata
    10921 tgattttcag actctagcag acttgaaata gaaggcatct cctgaatgcg tttttcatag
    10981 cgagaaattt cttcgtcaga agcacctggt agcacctgaa gcatgaatcc acctgcaact
    11041 ttaaccttat cctcgtcatc taagaggaca tttaaaccga cggcagaagg tgtttgctca
    11101 ctttcggtta aataataagc aaaatcttct ccaatttctc ctgtaattag tggagttgtt
    11161 gaagtgtaag gttgcccagt tccgtaatcg gtaataacga caaagtgtcc attccccatg
    11221 aaaggtccaa ctaagacctc tccagtagct gttttcttga tatcaacacc cgtattttgg
    11281 atgtaacctt taacatttcc tttagtatca gctacagaaa taatatgacc aaaagagcta
    11341 tctccaataa ccttgacagt tactttacta tttccttttt gatttgctgc taaaatttga
    11401 tttgcaatta atgttctccc aagagctaca gtagaacttg aaagtgtctg atgtttttct
    11461 tgagctgttc ttacagtttc tgtacaatca aggacatagg cccggaaaga accggaagtt
    11521 gaaattgatt ttataatttt atccataagt catattatag cataaaaggc ttattttgca
    11581 ttggagatag tcaggaaaat atagatgaaa acggtataaa ttaatgatat aaaaacgata
    11641 ctgtgtttta cagtatcgtt tttatatagt ggatatgata gtaagttaag actcatcaac
    11701 tttttcttgg gtagatttac cttcgttgag taaggcttca actatttttt tatcacgaat
    11761 tttgacagaa tcattgattg tttcagccga tttaataaca gcagattcta attgggcacg
    11821 ttccttacgt cctttgtcta tggcagcgat aataccatta ttttgagcca ctaagctttc
    11881 agctaatgca gtgacagatt taatagaaac agtggggctt tgtgcggtct tctctaacat
    11941 cggaatcgct tctttactag tttcagccag catttgcaat gctgcattat tagcgttgac
    12001 aatagcatca gcagtgacac cggatttgac agattgttgc atcatgccta actgagcgat
    12061 tgagagtttc attgttggaa tggtatttcg acgtaacata ccaagtttct gacgcatatc
    12121 tgacgatact ttgaccaagt ttcgcatctg tggtgttgtt gcccatgcaa cgtagagacg
    12181 gctgacatat tccgtatgtt gctgttcgag ggtattgata acttcagtca ttcgggctaa
    12241 ttggttactt ttaatttgat attcggacgt ttggctatct aatgctagaa tttcttgttg
    12301 taagtggctt gcacgattgg cagcctcggc ttgactcgat tcaataaaag caataactcc
    12361 aaccaaattt tcaatagatt tagtattatc ttcaatgagc atttcagcag agacgatatt
    12421 tcttgccaaa gtatcttctt gtttgacaac atttgctgcc atcatatcca ttttttgctc
    12481 gatgttttgt gagtcaaaat aaaattcctg tagcgaggtc ttgctttgtt tgaataattt
    12541 ttgaatcaag tttggttttt tctctaattc tgccggagta gcatctttat atttggcaat
    12601 aaatccattt agttcgcgat tagcattttt tagtaaatca tcaacttgag gaatttgaat
    12661 ttttttctgc tcagacaaga tatgattaac agtagtatta acgccttcta ctgcggattg
    12721 tccaaaatca aggagcgcat tttggtcacc gacaaaagta tctaccaaag ctggtgtttt
    12781 ttcagagata gcagactttt gtgctggtgt tagtttttca aaaaaggcaa tttgcccagt
    12841 ttggcttgtt gtctggttgg aaataatttc tgttgtttta tctgttttag tgatagcatt
    12901 gtctgcaatt tggtcaatat caaaattaaa ggtatcgctc atttattgtt cctttcgtct
    12961 tgcatcattc ttaggctaac gtcaaaatct ttcatatcac tttcattcaa ttggcgcagc
    13021 gtttcatcta aatctaaatt aaatttttta attgcttgta atgcttgttc taggcgttcc
    13081 ttagcattgt taaaattttt aggagattgt ttgattctga tgtagccagc cagaacatct
    13141 gtaaaacgtt tcatattggc ttgatgaatt gctgctaatt cagctttgtt atctgcctct
    13201 tcaatctttt ttaagatagc atagtgatca tcttgaatat tgtgatagaa cggttttaat
    13261 tccggagcaa tgcgtatcat ttcatcttcc tgatcagaaa taggttctag ttctggagag
    13321 gcatgtagct ttattaattg ttcgttaatc tcattactaa atgtatttgc ttttttggtg
    13381 atacgtttat agatattagg ctcaatggct ttttgtagtt ggtttgcttc attacgaatt
    13441 aggtccaatt gaggtaatat ttgatgtgct aatttgatat attccttaga acgattgtct
    13501 ttttttaaaa tttccaagtg tctaatgtct tggtcagtct ctttaatatc ggattttaaa
    13561 gcttcaatac gttggtttga tgtaaaacat ttaagacgct ttttatcata aacgtaatag
    13621 gcacaataac caccaccaat aattaacaga gtagcaatca cctcactcca aaagtctgaa
    13681 atcagggaag caagaacaac aataccaagt aatgttatta gattatgaga atttttggaa
    13741 tagcgttttt taaccataat aatatttaac ctaactttct gtagttgtta atattttatc
    13801 ttaaatgaac tagtgatgac aagggataac tttattttaa ccaaaaattc cttaagctta
    13861 gcctaaggaa ctctttttag ttataattct gggaacagtt tagccaaaat tcgaggtgta
    13921 atagattgac caggcccctc ttgacagtag gtgtgcatat acatcaatgg gttaaagttt
    13981 agttcaatgc aagtagcatt tttcttatct tttgagtatg cttgagtggc attaggaata
    14041 attaaatcaa ctccacaaac ccaagccccc atagcctctg ccatctcagc tgctagttgt
    14101 ttatatgtag gatccattgt atttgttaca tctatagagt caccgccagt ggaaatatta
    14161 gaattacggc gaagttctat ttttgttcct tctggaggga tactgttaac agtgtatcct
    14221 tgttgttcca acattaactg ttctacttct ccgagctcaa taacctctag aggagagcga
    14281 tgatcatagc ctctgagagg gttttggttt ttaagtttaa ctagctgact aatagtatga
    14341 ataccatctc caacgacatt agctgctacc ctaagcaata cagcaataca gtccccctca
    14401 aggacgaaga aacggtattc ggtaccctca atatattctt ccacaagaat agcgctatct
    14461 tcggcaaaag caatgtcaat tgctttttca taagaagcta agttagctga tgttttaaaa
    14521 atagaaatgc ctaaaccaaa gtttgtagat tttggcttaa cgacgattgg tttatcttgg
    14581 atttgtgaaa aatagttaag tgcctcttta cggtcagtaa attcatctcc aaaaggagtt
    14641 gggaaatgct tttcatctag gatttttttt gtaacaactt tgttagccat agcgagcggt
    14701 acaatatagt tatcttttga ggtcatatta ccatttttaa cgtactcaat atgactattg
    14761 tgccataatt ttaggaattg atcttgttca tccagtactt cgaagttgac tcctttttgg
    14821 ataacgtcaa atagtagcaa ttgggtagaa agttccatcg tttcatatcc ctttaaggcg
    14881 tagggagctt cccaagcata atcgtgataa atttgtcctt gtctttgtcc aaatgtttct
    14941 aaggaaagtc cttcaatcat ttctaaaagt tgaccagcta ccgttaattt aggtgactga
    15001 atttgccttt ttacagattc caataggtct tgatagtaag gtggtaagtt aaaatgttgg
    15061 ataacagatt gcatagcatc cactaagtca gaaactggtg cttgattagg taatttttct
    15121 aatggatgac tgagtgctat aaggtcattt aatctattgg cttccttgat atcttggtca
    15181 atatgactac tagaatctat ccacaaaagc gccaataaga aaaggtgaac agtatcaaca
    15241 gtttcctgcg ttataccgat tgggctaaat gggttgagat caaaagtgcg aaattctaaa
    15301 tatgtaattc ctttttctaa ataattacga caggacttac taccacggag acgaacaggt
    15361 gaataaaatt ctttctcagc aatcaattgc ccacttttta cagcattttc tagatcgttg
    15421 acataatcct tcaagctagt ataagaaata cgaatatctt tatgattcac atagcctaag
    15481 tgactatttc taagtgaccg gacagggtta ttaagtttct gatttaaaaa gtcttcttca
    15541 gctactgggc tcgctccgta tagataagtt agtaaccaac ggtaacgtaa aaaattttga
    15601 gacaatttca tgtaaagctg gttctgaaaa tcaatagcat tatctgcttg actcaattca
    15661 aagagagaag ttaataactc ttgacctaaa ccgagattat agtgaattcc agagatggat
    15721 tgtattagtt ttccataagt tttctccaaa tatttgcgat aatcatattc aaaagcatcc
    15781 tctaattggg caatctgtat gtcttcttcc ctcactttgg gtggcataga aagcggccat
    15841 aagtattcat cgtggttgat agatcgtcca gcgacatcac ttatagcttt caaaaatcga
    15901 atggcttctt ggctatcctt tgcaataggt gtaatgagtt ctaattgagg ttcactataa
    15961 tccgtttgga tataaggatg atagttacga ctccctaatg ttttgggatg gggtgtttga
    16021 gcaacccttt gagtgggttg gtgaatacga aggctttctc tctctaagcc aaatgtagct
    16081 tgtagaatcg gtagatgaga gtggcttctt tgtaacagtt gatcgataat cacgacaaca
    16141 ttcctcctag taacagatga tactagttta tcaaaaaaat aat
//
LOCUS       DATRUK010000047        16158 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.047, whole genome shotgun sequence.
ACCESSION   DATRUK010000047 DATRUK010000000
VERSION     DATRUK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16158
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.047"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..651)
                     /locus_tag="VBE34_001417"
     CDS             complement(<1..651)
                     /locus_tag="VBE34_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7264130.1"
                     /translation="MLTHKNILLTIIFGLFMIILSACGMSNKEMAGIDNWEHYQKEKK
                     ITIGFDNTFVPMGFESRSGDYTGFDIDLANAVFKEYGISVKWQPINWDMKETELNNGN
                     IDLIWNGYSKTAERAKKVAFTNPYMNNHQVIVTKTSSHINSIKDMKGKKLGAQSGSSG
                     FDAFNAKPDILKKFVKGKEAVQYDTFTQALIDLKNNRIDGLLIDEVYANYYLKQEGN"
     gene            complement(665..1294)
                     /locus_tag="VBE34_001418"
     CDS             complement(665..1294)
                     /locus_tag="VBE34_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7264131.1"
                     /translation="MLELKNISKCYGQKEIFKDFNLTVEEGKILSLVGPSGGGKTTLL
                     RMLAGLEKIDSGTIVHDGKEVSVDHLETLNLLGFVFQDFQLFPHLTVLDNLILSPVKT
                     MGLSKELAKEKALVLLERLGLKDHALVYPFSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQEVEKLILQNRETGMTQIVVTHDLQFAESISDTILKINPK"
     gene            complement(1304..1945)
                     /locus_tag="VBE34_001419"
     CDS             complement(1304..1945)
                     /locus_tag="VBE34_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7264132.1"
                     /translation="MSYILEILPSLLVGAQVTLQVFFIVICLSIPLGIVVAFLMKVHF
                     KPLQWLLTTYIWIMRGTPLLLQLIFFYYFLPSVGISFDRMPAAIIAFTMNYAAYFAEI
                     FRGGIESIPKGQYEAAKVLKFSKFQTVWYIVLPQVFKIVLPSVFNETITLVKDSSLVY
                     ILGVGDLLLESKTAANRDATLAPMFIAGGIYLLLIGLLTILSKQVEKRFNYYK"
     gene            complement(2080..2409)
                     /locus_tag="VBE34_001420"
     CDS             complement(2080..2409)
                     /locus_tag="VBE34_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEO7264133.1"
                     /translation="MSLPNCPKCNSEYVYEDGILLVCPECAYEWNPEEIEEEVGLIVL
                     DSNGTRLSDGDTVTVIKDLKVKGAPKDIKQGTRVKNIRLVDGDHNIDCKIDGFGAMKL
                     KSEFIKK"
     gene            complement(2572..4386)
                     /gene="glmS"
                     /locus_tag="VBE34_001421"
     CDS             complement(2572..4386)
                     /gene="glmS"
                     /locus_tag="VBE34_001421"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEO7264134.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVVGDNKSQL
                     VKSVGRIAELQAKVGDSVSGTTGIGHTRWATHGKPTEGNAHPHTSGSGRFVLVHNGVI
                     ENYLQIKETYLTKHNLKGETDTEIAIHLVEHFVEEDNLSVLEAFKKALHIIEGSYAFA
                     LIDSQDADTIYVAKNKSPLLIGLGNGYNMVCSDAMAMIRETSEYMEIHDKELVIVKKD
                     SVEVQDYDGNVIERGSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRKLISTYANESG
                     DMNVDSDIIKSVQEADRLYILAAGTSYHAGFAAKTMIEKLTDTPVELGVSSEWGYNMP
                     LLSKKPMFILLSQSGETADSRQVLVKANEMGIPSLTITNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQVATLAFLAKAVGEANGKAEAKDFDLVHELSIVAQSIEATLSEKD
                     VISEKVEQLLISTRNAFYIGRGNDYYVTMEAALKLKEISYIQTEGFAAGELKHGTISL
                     IEDNTPVIALISADSTIAAHTRGNIQEVVSRGANALIIVEEGLEREGDDIIVNKVHPF
                     LSAISMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            complement(4655..5212)
                     /gene="lepB"
                     /locus_tag="VBE34_001422"
     CDS             complement(4655..5212)
                     /gene="lepB"
                     /locus_tag="VBE34_001422"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO7264135.1"
                     /translation="MVKRDFIRNIILALIAVLILILLRYFVFATFKVHKDATNSYFSN
                     GDVVVVNRNRTPKYKDFIVYKVGKIFYISRVIGEPNQKVRVMDDILYLNDVFKDEPYI
                     EKMKNAYSEKKDGQMPFTSDFSVETLTRNKESRVPKGSYLVLNDNRQNKNDSRKFGLI
                     KEKDIRGVITFKVYPLSEFGFTASE"
     gene            complement(5383..6885)
                     /gene="pyk"
                     /locus_tag="VBE34_001423"
     CDS             complement(5383..6885)
                     /gene="pyk"
                     /locus_tag="VBE34_001423"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEO7264136.1"
                     /translation="MNKRVKIVATLGPAVEFRGGKKFGESGYWGESLDVEASAEKIAQ
                     LIKEGANVFRFNFSHGDHAEQGARMATVRKAEEIAGQKVGFLLDTKGPEIRTELFEDG
                     ADFHSYTTGTKLRVATKQGIKSTPEVIALNVAGGLDIFDDVEVGKQILVDDGKLGLTV
                     FAKDKDTREFEVVVENDGLIGKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVNEVRAICEETGNGHVKLFAKIENQQGIDNIDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIITKVNAAGKAVITATNMLETMTDKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDKNAQTLLNEYGRLDSSAFPRNNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRSLMINWGVIPVLAD
                     KPASTDDMFEVAERVALEAGLVESGDNIVIVAGVPVGTGGTNTMRVRTVK"
     gene            complement(6934..7956)
                     /gene="pfkA"
                     /locus_tag="VBE34_001424"
     CDS             complement(6934..7956)
                     /gene="pfkA"
                     /locus_tag="VBE34_001424"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEO7264137.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINQGYYGM
                     VTGDIFPLDANSVGDTINRGGTFLRSARYPEFAELEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGLPGTIDNDIVGTDYTIGFDTAVATAVENLDRLRDTSASH
                     NRTFVVEVMGRNAGDIALWSGIAAGADQIIVPEEEFNIDEVVSNVRAGYAAGKHHQII
                     VLAEGVMSGDEFAKTMKAAGDDSDLRVTNLGHLLRGGSPTARDRVLASRMGAYAVQLL
                     KEGRGGLAVGVHNEEMVESPILGLAEEGALFSLTDEGKIVVNNPHEADLRLAALNRDL
                     ANQSSK"
     gene            complement(8037..11141)
                     /locus_tag="VBE34_001425"
     CDS             complement(8037..11141)
                     /locus_tag="VBE34_001425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEO7264138.1"
                     /translation="MFAQLDTRTVYSFMDSLVDLKTYVSKSKSLGYQTIGILDHSNLY
                     AAYHFIQEAQKANLRPIVGFSFDIVVENRSIEVYCIAINTVGYKNLLKLSTAQMSEKM
                     SLNLLTEHLEGVQLILPYQDALDQLNLPFDYVIGVNLTSPQIPYTKPIIAIDTVRYFQ
                     KNDIETLQMLHAIRDNVLLKDAQYASKNQELKPCQEMTLAFKKRFPEALANLESLVEN
                     VVYHFDSDFKLPIFNREIPAGEELRTLTQNNLKSKGLWSDAYQERLEKELTIIHKMGF
                     DDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVSYALNITGIDPVKHNLIFERFLNEE
                     RYSMPDIDIDLPDIYRGEFLRYVRNRYGSMHSAQIVTFSTFGAKQAIRDVFKRFGASE
                     YELTNITKKIHFRDNLTSVYNRNLAFRQIIDSKIEYQKAYDIAKRIEGNPRQTSIHAA
                     GVVMSDDLLTDHIPLKNGEDMMITQYDASSVEANGLLKMDFLGLRNLTFVQKMKEKVD
                     KDYGISIQLEAIDLEDKETLKLFAAGQTKGIFQFEQSGAINLLRRIRPECFEDVVATT
                     SLNRPGASDYTENFINRRFGKEKIDLVDPVIAPILQPTYGIMLYQEQVMQIAQTYAGF
                     TLGKSDLLRRAMSKKNSKEMQKMSQSFLEGAVSKGHRQEDARLIFERMAKFAGYGFNR
                     SHAFAYSALAFQLAYFKAHYSDVFYDIMINYSNSDYLIDAIDFGFVIEKPSINTISYR
                     DRIYKKKIYLGLKNIKAVPNDLAYWISKNQPFQSIEDFLMRLPQQFQKSGFISPLIAI
                     GAFDEFDNNRRKITSNLDSLFTFVNELGSLFADTSYHWLEVEDFSNSEKYEMEQDILG
                     VGISPHPLVGISQKASRPFIPISQVQENSEATILVQLKQVKIIRTKSSGQQMAFLTVM
                     DINSKMDITVFPETFNIVRDDLQEGKYYYLHGKIQKRDERLQMVLNGVQEATEERFWI
                     LLKNHDNDKKISEILSKYKGHIPVYLHYETTKETIQSKVHLVRKDSGLALDLSEFVVK
                     TVYQ"
     gene            11286..11654
                     /locus_tag="VBE34_001426"
     CDS             11286..11654
                     /locus_tag="VBE34_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7264139.1"
                     /translation="MAWEFNEKSPIYSQIAEHIKMQIVSQEIKSGDQLPTVRELAQEA
                     GVNPNTMQRAFTELEREGMVFSQRTSGRFVTEDNLLIGKIRQQVAKAELATFVNNMKK
                     IGYKLDEITVALDHFIKEIN"
     gene            11655..12353
                     /locus_tag="VBE34_001427"
     CDS             11655..12353
                     /locus_tag="VBE34_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7264140.1"
                     /translation="MTQLLQLHHVTKKYHKHIAVNDVTVSIPTGKIIGLLGPNGSGKT
                     TIIKMINGLLQPDKGDIVIDGYRPSVETKKIISYLPDTSYLQENMKIKDVVTLFEDFY
                     NDFNSKVAYQLFEDLNLNPKERLKNLSKGNKEKVQLILVMSRKARLYILDEPIGGVDP
                     AARDYILKTIISNYSNDASVLISTHLISDIEPILDEVIFLKEGEIDLQGNADDLREEH
                     NCSIDALFRERFKK"
     gene            12365..13147
                     /locus_tag="VBE34_001428"
     CDS             12365..13147
                     /locus_tag="VBE34_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter (permease)"
                     /protein_id="HEO7264141.1"
                     /translation="MFGKLLKYELKSVGKWYLTLNAAVLLVSIILGLVLKALGGNFST
                     DTNSTSAQIFTIILVLLLAMVISGSLLSTLAIIIKRFYSNIFGRQGYLTLTLPVTTNQ
                     IICSKLLASLLWSIFNIFIVIIGIILVILPLVGIGQFVVAFPEIYKIISSSNAPLFIA
                     YFFLSYVAGTLLIYLSIAVGQLFTNKRVLMGIVSYFGISLLITFLTLIIDSIFHIDLF
                     NSHANATFSQPVLLYNILVSIVEIAIFYMLTHSIIKYKLNIQ"
     gene            complement(13184..13795)
                     /locus_tag="VBE34_001429"
     CDS             complement(13184..13795)
                     /locus_tag="VBE34_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEO7264142.1"
                     /translation="MNMKLSKRYRFWQKVIKALGVLALIATLVLVVYLYKLGILNDSN
                     ELKDLVHKYEFWGPMIFIVAQIVQIVFPVIPGGVTTVAGFLIFGPTLGFIYNYIGIII
                     GSVILFWLVKFYGRKFVLLFMDQKTFDKYESKLETSGYEKFFIFCMASPISPADIMVM
                     ITGLSNMSIKRFVTIIMITKPISIIGYSYLWIYGGDILKNFLN"
     gene            13888..14237
                     /gene="ssrA"
                     /locus_tag="VBE34_001430"
     tmRNA           13888..14237
                     /gene="ssrA"
                     /locus_tag="VBE34_001430"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14506..15144
                     /locus_tag="VBE34_001431"
     CDS             14506..15144
                     /locus_tag="VBE34_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEO7264143.1"
                     /translation="MASILKKHLEKRLELKNDPMRTDMSTGASYPKYGFSVGKYTYGY
                     QQFFYEGVNLKEIGAFCSIAQNVTITGMNHPTDHITTNPFIYYKSRGFINEDRADLID
                     EKKNGKVIIGNDVWIGTNVTILPSVTIGNGAIIEAGSVITKDIPDYAVVAGTPAKIIK
                     YRFSEEEITLLNASQWWNWSDEAIKEHISEFSDKREFFNTLKSISENKNHKL"
     gene            complement(15262..>16158)
                     /locus_tag="VBE34_001432"
     CDS             complement(15262..>16158)
                     /locus_tag="VBE34_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO7264144.1"
                     /translation="FTSQRHDLCQLMMTSKRFKNLTLSYYQAEISLEFEKQFAAMVFT
                     IPNIDYHQVVFRGTDANLIGWKEDFKLTYMREISAHRSAIKYLNTILPYFDKVVLSGH
                     SKGGNLALYAAMFTKPDLKAKIDLIWLIDSPGLQKTLLPTTEYKTTKQKCIRLLPEES
                     IVGMMLYSDIEPLIISSNARGILQHDVTTWEIQEPAILKTGAGLSLKSICFEKTFQQW
                     MAELKSQERKLFFDLLFDSFLSSGVSSLDDFNLASRAKMMKAFHSFRELDDDKKRLFN
                     KSLKLLVTIFWGAYHDNSRETK"
BASE COUNT         5308 a         3090 c         2466 g         5294 t
ORIGIN      
        1 atttccttct tgctttaaat aatagttagc ataaacttca tcaatcaaaa gaccatcaat
       61 acggttattt tttaaatcaa tcaaagcctg agtgaaagta tcgtattgaa ctgcttcttt
      121 tccttttaca aactttttta aaatatcagg tttagcgtta aaagcatcaa aaccagatga
      181 acccgactgg gctcctagtt ttttcccctt catatcctta atactattaa tatgtgatga
      241 agttttagta acaattactt gatgattatt catatatggg tttgtaaaag cgactttttt
      301 agcacgttct gccgtttttg aataaccatt ccaaataagg tctatattac cattattaag
      361 ttcagtttct ttcatatccc agttaatagg ctgccatttc actgaaatac cgtattcttt
      421 aaaaacagca ttagctaaat caatatcaaa gccggtatag tcaccagaac gactttcaaa
      481 tcccatagga acaaaagtat tatcaaatcc aatagtaatt ttcttttcct tttgataatg
      541 ttcccaatta tcaataccag ccatttcctt attagacata ccacatgctg ataatataat
      601 cataaataat ccaaatataa tggttaataa tatatttttg tgagtcaaca atctaccccc
      661 aagtctactt aggattaatt ttgagaatcg tatcagatat actttcagca aattgaagat
      721 catgtgttac tacaatttgt gtcatacctg tttctctatt ttgtaaaatt agtttttcta
      781 cttcttgacg caactctgga tcaagagcgc tagttggctc atcataacca ataatctgtg
      841 gatcaatcat catcgcacga gctagtgcga cacgttgctt ttgcccccca gataatgaaa
      901 atggatagac taaagcatgg tctttaagtc ccaagcgttc aagtaatact aaggcttttt
      961 ctttagcaag ttcttttgat aatcccatcg ttttaacagg agagaggatc aaattatcta
     1021 atactgttaa atgtgggaat aattgaaagt cttggaagac gaatcctaaa agatttagtg
     1081 tttccaaatg atcaacagat acttctttac catcgtgaac aatagtccct gaatcaatct
     1141 tttccaatcc agctaacata cgtaatagtg ttgtttttcc acctccagaa ggtcctacta
     1201 aagagagaat tttaccttct tctacagtca gattgaaatc tttaaagatt tctttttgcc
     1261 catagcattt agaaatattt tttaattcta acatcgtccc tccttattta tagtagttaa
     1321 aacgcttttc aacttgttta gataaaatag ttaaaagacc aattaataat aggtaaatac
     1381 cacctgctat aaacattggt gcaagtgttg catccctatt tgcggctgtc ttactttcta
     1441 gaagtaagtc gcccacacca agtatgtaaa ccaatgagga atctttcact agagtaatcg
     1501 tttcattgaa aacactcggt aagactattt tgaagacttg aggtaatact atgtaccaaa
     1561 ccgtttgaaa cttagaaaac tttaatacct ttgcagcttc atattgccct ttaggaatag
     1621 actcaatacc tcctcgaaaa atctctgcaa aataagctgc ataattcatc gtaaaagcaa
     1681 taatcgcagc tggcatacga tcaaaagaaa ttcctacact tggtaaaaag taataaaaaa
     1741 agataagctg taataacaga ggcgtgcctc gcataatcca aatataagtt gtcagtagcc
     1801 attgaagcgg cttaaaatga actttcatta ggaaggctac aacaatacca agtggaattg
     1861 ataaacaaat aacaataaag aaaacttgca atgtcacttg cgcacctact aataagctag
     1921 gtaagatttc taaaatataa gacatggtcc cccctaaata aaaaatagat gttctcatta
     1981 tatcaaaatt caaaccaatg tcatagagct tttttaataa aaatgcatga aatcaagtca
     2041 tattcaaaaa gcaattgacc gtcgccaatt gcctgaaagc tattttttta taaactcaga
     2101 ttttagtttc atagctccaa aaccatcaat tttacaatcg atattatgat cgccatcaac
     2161 taaacgaata tttttaacac gagtgccttg cttaatatcc ttaggcgctc cttttacttt
     2221 taaatcctta ataactgtca cagtatctcc atctgataag cgcgttccat tgctatccaa
     2281 tacaattaaa cctacttctt cttcgatttc ttctggattc cactcgtaag cacattctgg
     2341 acaaaccagt aaaataccgt cttcatagac atattcagag ttacacttag ggcaatttgg
     2401 taatgacatt tttctctcct taaattaatt aataaaatag tcagcaagta acattctaac
     2461 tgattatagg cttcttgaca ataggaaaaa gcctattttc cttcaaaaat aggctttttt
     2521 acaattttta atattttgta gttaatatta gcgtatttta gtgtaacgag tctattcaac
     2581 agttacagct tttgccaaat tacgtggttt atcaacatct aagccacgtt gtaatgaagc
     2641 atagtaagca attaattgag tcggaataac cattgaaatg gctgataaga atgggtgaac
     2701 tttattaaca ataatatcat caccttcacg ttccaaccct tcttctacaa taatcaaagc
     2761 atttgctccg cgagaaacaa cttcttggat atttccacga gtatgcgctg caatagttga
     2821 atcagcagat attaaagcaa taacaggagt attgtcttca atcaacgaaa ttgtaccatg
     2881 ttttaattca cctgcagcaa aaccttctgt ctgaatataa gaaatctctt ttagcttcag
     2941 tgcagcctcc attgtaacat aatagtcgtt accacgacca atgtagaagg cattacgtgt
     3001 cgagattaac aactgctcta ccttctcaga gataacatct ttttctgata aggtcgcttc
     3061 aattgattgt gctacaattg acaattcatg aaccaaatca aaatcttttg cttctgcctt
     3121 accatttgct tctcctaccg cttttgctaa gaaagctaac gttgcaactt gagcagtata
     3181 tgcttttgta gaagcaacag cgatttcagg tcctgcatga atgagcatcg tataggttgc
     3241 ttcacgagaa agtgttgagc caggcacatt tgttatagtt aaactaggga ttcccatttc
     3301 gtttgcttta actaaaactt gtctactatc tgctgtttct cccgattgac taagtaaaat
     3361 aaacattggc ttcttactca aaagtggcat gttgtaaccc cattctgaag aaactccaag
     3421 ttcaacaggt gtatcagtta atttttcgat catggtctta gcagcaaaac cagcatgata
     3481 tgacgttcct gcagccaata tgtaaagacg atctgcttct tggactgatt ttataatatc
     3541 agaatccaca ttcatatctc cagattcgtt cgcataagtt gaaatcaact tacgcatcac
     3601 agttggttgc tcatcaattt ctttaagcat gtagaatgga taagtcccct taccaatatc
     3661 tgataagtca agctctgctg tgtagctacc acgttcaatg acattaccat cataatcttg
     3721 aacttcaacg ctatcttttt taacaataac gagttcttta tcgtggattt ccatatattc
     3781 acttgtctca cgtatcatag ccatggcatc tgaacacacc atattataac catttcccaa
     3841 acctattaga agaggtgatt tatttttagc tacgtaaatc gtatcagcat cttgagaatc
     3901 aataagagca aatgcgtatg atccttctat aatatgtaag gcttttttaa aggcttcaag
     3961 cactgataag ttatcttctt caacaaagtg ttcaactaaa tgaatagcta tttctgtatc
     4021 agtttcaccc ttgaggttat gttttgttaa gtaagtttct ttaatttgaa ggtaattttc
     4081 aataacacca ttgtgtacca atacaaaacg accagatcct gaagtgtgag gatgtgcatt
     4141 tccttcagta ggtttaccat gggttgccca acgtgtatga ccgatacctg tcgtaccact
     4201 aacagaatct cctacttttg cctgaagctc cgcgattcga ccgactgact tcacaagttg
     4261 acttttgtta tcacctacta caaaaatacc tgctgagtca tatcctcggt actctagctt
     4321 ttctaagcct tgaattaaaa tatccgttgc atttgtattt ccaactacac ctacgatacc
     4381 acacatatta ttattaccaa taagagcttt ctcttatact ttctatctaa actttctttt
     4441 tgaattggta tatattaata tataccgtta aaaagtaaca aaaacagtat ataattaatg
     4501 tttttgtttg tcaataaaaa aattggtata gtctttttga ttttctgtga taacgagata
     4561 aacgctatca aaaattctat ctatcttaaa aaaatatatt tcttagccat tttattacat
     4621 aaaaagaatg gtatcaaata ataccattct tttactactc tgatgctgta aaaccaaatt
     4681 cacttagagg gtatacttta aaggtaataa caccacgaat atctttctct ttgattaatc
     4741 caaattttcg actatcattt ttattttgtc gattatcatt taacacaaga taagaaccct
     4801 taggaactct gctttcttta tttcgtgtta atgtttcaac tgagaagtcg gaagtaaagg
     4861 gcatttgacc gtctttcttt tcagaataag catttttcat tttttcaatg taaggctcat
     4921 ccttgaaaac atcattcaaa tataaaatat catccattac tctcactttt tgatttggct
     4981 caccaataac acgactaata tagaaaatct ttccaacctt atagacaata aagtctttgt
     5041 acttaggcgt cctattacga ttaacaacaa caacatctcc gttgctaaaa taagaatttg
     5101 tagcatcctt atgtacttta aaggttgcaa aaacaaaata tctcaagaga atcaaaatca
     5161 atacagcaat caatgctaaa ataatatttc taataaaatc gcgtttaacc ataactactc
     5221 ctaatctttt ttccattata tcatgttttc atagataaaa aaaggagggt aattattaat
     5281 atcatatcct attctagtca caataatcga ataataaaaa gcaaaacata taaaaaaccc
     5341 attatttaag gtattcttaa ataataggtt tgaattcaaa aattatttaa cagtacgaac
     5401 acgcattgtg ttagttccac ctgtacctac aggaacacct gcaacgataa cgatattatc
     5461 gcctgattca acaagtcctg cttcaagtgc tacacgttct gcaacctcaa acatatcatc
     5521 tgtagatgct ggtttgtctg caaggacagg gataacaccc cagttaatca tcaatgaacg
     5581 ttgtactttt tcatcaaatg taacagccaa aatgtctgca tctggacgga acttagaaat
     5641 ggcacgagct gtattacctg tttcagtaat tgttacaaca agtttgatat ccattgagtg
     5701 tgttgcatct ttaaccgcag atgcaataac atcagttttg ttattacgtg ggaatgcaga
     5761 tgagtctaag cgaccatact cattgagtaa tgtttgagca tttttatcaa tagtagccat
     5821 tgtacgaact gactcaactg ggtatttacc attagctgac tcacctgaaa gcattgtagc
     5881 atcagtacca tcaataacag cattgaagac atcagatact tctgaacgag tcgcacgtgg
     5941 tttatcagtc attgtttcaa gcatatttgt tgctgtaata actgctttac cagctgcatt
     6001 aactttagta atgatcattt tttggtaaac tggaaccatt tcaaatggaa cttcgatacc
     6061 catatcacca cgagcaatca taataccatc tgctgcttcg ataatctcat caatattatc
     6121 gataccttgt tgattttcaa ttttagcaaa caacttaacg tgtccattgc cagtttcttc
     6181 acaaatagca cgaacttcat taacatcttt agcagtacgt acaaatgaga tagcaataaa
     6241 gttaagtcct tgctcaagtc caaaacggat atcagcatta tcgcgttctg caagtgctgg
     6301 gaaaggaatt ttagtataag ggatgtttac acctttttgt ttaccaataa ggccatcatt
     6361 ctcaacaact acttcaaatt cacgagtgtc tttatctttt gcaaacacag taagacctag
     6421 tttaccatca tcaacaagga tttgcttacc aacttcaacg tcatcaaaga tgtcaagtcc
     6481 accagcaaca ttcaatgcaa tcacttctgg agttgatttg ataccttgct tagtagcaac
     6541 acgtaatttt gtacctgttg tatatgaatg gaaatctgca ccatcttcaa aaagttctgt
     6601 acgaatttca ggtcctttag tatcaaggag gaagccaact ttttgtcctg caatctcttc
     6661 tgctttacga acagtagcca tacgagctcc ttgctcagca tgatctccat gtgagaagtt
     6721 gaaacggaaa acgttagcac cttctttaat caattgagca attttttctg ctgaagcttc
     6781 tacgtcaagg ctttcacccc agtatccaga ctcaccaaac ttcttaccac cacggaattc
     6841 taccgcagga ccaagtgttg caacgatttt tacgcgttta ttcataataa tttatgagct
     6901 cctttttatt taaagtgtca aattgacaaa ttcttattta ctactttggt tggcaaggtc
     6961 acgattaagt gctgccaagc gaagatccgc ttcatgcgga ttattaacaa cgatttttcc
     7021 ttcatcagtc aagctgaaca aagcaccttc ttctgctaaa cctaaaattg gactttcaac
     7081 catttcttcg ttgtggacac caacggctaa accaccacga ccttctttca acaattgaac
     7141 agcgtacgct cccatacgag atgctaagac acgatcacga gccgtcggac taccaccacg
     7201 gagcagatgt cctaaattcg tcacacgaag atcgctatcg tctcctgctg ctttcattgt
     7261 ttttgcaaac tcatcaccac tcataacacc ttctgcaaga acgatgattt ggtgatgttt
     7321 accagctgca tagccagctc taacatttga gacaacttca tcaatattga actcttcttc
     7381 aggaacaata atttgatctg cacctgcagc gatacctgac caaagagcga tatctcctgc
     7441 atttcttccc ataacctcaa caacaaaagt acggttatga cttgctgatg tatcacgaag
     7501 acggtcaaga ttctcaactg ctgtcgcaac tgctgtgtca aaaccaatag tatagtcagt
     7561 gccaacgata tcgttatcaa ttgtacccgg caaaccaaca gctgggaaac cgtgctcagt
     7621 tagacgcata gcaccatgat aagaaccatc accaccgata actactacac cttcaatacc
     7681 gtgtttttta agctgttcaa tccctttaag ctgaccttca agttcagcaa attcaggata
     7741 acgggctgaa cgtaaaaacg ttcctccacg gttgatagta tccccaacag aattagcatc
     7801 caaagggaaa atatcccctg tcaccatacc atagtaacct tggttgatgc cgtaaacttc
     7861 cataccttca gaaattgctt tacgaacaac tgcacggata gcagcgttca taccaggggc
     7921 gtcaccacca ctagttaaaa cagcaatacg tttcatttaa ttggtactcc ttttattaat
     7981 ttaacgattt aattatagca tattaatttg taaatttctg cactttatat gcaattttat
     8041 tgataaaccg ttttcacaac aaattcagat aaatcaaggg ctaagccact atcttttcta
     8101 accaaatgta ctttgctttg gatagtttct ttagttgttt cataatgcaa atagacagga
     8161 atatgccctt tatatttgga caatatttcc gatatttttt tgtcgttatc atgatttttt
     8221 agaagtatcc aaaaacgttc ctccgttgct tcttgaacac cgtttaaaac catctgtaat
     8281 cgctcatctc gcttctgaat ttttccatgc aagtaataat atttcccttc ttgtaaatca
     8341 tctcttacaa tattgaaagt ttcaggaaaa acagtaatat ccatttttga attaatatcc
     8401 ataacagtca agaaagccat ttgttgtcca gaactttttg tcctaatgat tttgacctgc
     8461 tttagctgaa ctaaaatagt agcttcgcta ttttcttgga cttgactaat tggtataaac
     8521 ggacggctag ctttttgtga aatccccact aaagggtgcg gactgattcc tactcctaag
     8581 atatcttgtt ccatttcata tttctcagaa ttagagaaat cttctacttc taaccaatga
     8641 tatgaggtat ctgcaaataa actacctagt tcatttacaa atgtaaataa agaatcaaga
     8701 ttagaggtga ttttccgtct attgttatca aattcgtcaa atgctccaat agcaattagc
     8761 ggagaaataa aaccactttt ttgaaactgc tgtggcagtc tcattagaaa atcttcaata
     8821 ctctgaaatg gttgattttt agaaatccaa taagccaagt cgttaggaac agccttaata
     8881 ttttttaaac ctaaataaat tttcttttta tagatgcggt ctctgtatga aatagtatta
     8941 attgaaggtt tttcaataac aaaaccaaaa tcaatagcat cgattagata atcactattt
     9001 gaataattta tcatgatgtc ataaaagaca tcggaataat gggctttgaa ataggctaat
     9061 tgaaaagcta aagccgaata agcaaaggca tggctgcgat taaacccata acctgcaaat
     9121 ttagccatgc gttcaaatat aagtcgagca tcttcttgac gatgtccctt agatactgca
     9181 ccctctaaga aggattgaga catcttttgc atctccttag aatttttctt agacatagca
     9241 cgtctgagca aatcagactt gcctagcgta aaacctgcgt aggtctgggc aatctgcata
     9301 acctgttctt gatagagcat tatgccatag gtaggttgta aaattggagc tatgacagga
     9361 tctactaaat cgattttttc tttaccaaaa cgacggttta taaaattctc agtataatca
     9421 cttgcaccag gcctattcaa gctggtagta gctaccacat cttcaaaaca ctctggccta
     9481 atacgtcgca acaaattaat agctccactt tgctcaaatt gaaaaatccc ctttgtttga
     9541 ccagccgcaa atagttttag agtctcttta tcttctaaat caatagcttc taattgtata
     9601 gagataccgt agtccttatc gactttctcc ttcattttct gaacaaatgt caaatttcga
     9661 agacctagaa agtccatttt tagaagacca ttagcctcaa cagaactagc atcatattgc
     9721 gttatcatca tatcctcacc gttttttaaa ggaatatgat ctgttaacaa atcgtcactc
     9781 attactaccc cagcggcatg aatagaagtc tgtctaggat ttccttcaat acgcttagca
     9841 atatcatagg ctttttggta ttcaatttta ctatctatta tttgtctgaa agctaagttg
     9901 cggttataaa cactggtcaa attatctctg aaatgaattt ttttagttat gtttgttaac
     9961 tcatactctg atgcaccaaa gcgtttaaaa acatctcgaa tagcctgttt cgcaccaaaa
    10021 gttgagaagg ttacaatctg tgcagaatgc attgaaccat atcgattacg tacatagcgc
    10081 aagaattctc cacgataaat atccggaaga tcaatatcaa tatctggcat cgaataacgc
    10141 tcttcattta aaaaacgttc aaaaattaaa ttatgtttga caggatcgat accggtaata
    10201 tttaaagcat atgagactaa acttcccgct gctgaaccac gtcccattcc catatagtag
    10261 cccttactac gaccaaatct taataaatcc cacacaataa ggaaatagtc atcaaagccc
    10321 attttgtgaa tgattgttaa ttctttttcg agacgctctt gataagcatc gctccataaa
    10381 cctttacttt ttaagttatt ttgagttaag gttcttaatt cttctccagc aggaatttct
    10441 ctattaaaaa taggtaactt aaagtcagaa tcaaagtgat aaacaacatt ctctaccaaa
    10501 gattctaggt tagctaaagc ttcaggaaaa cgtttcttga atgctagagt catttcttga
    10561 caaggtttta attcttgatt tttgctcgca tattgtgcat cttttaacag gacattatct
    10621 cttatagcat gaagcatctg taatgtctca atatcatttt tttgaaaata tcgaacagta
    10681 tctatggcta taataggttt ggtatatgga atctgcggag aggtcaaatt aacaccaata
    10741 acataatcaa agggtaaatt gagctgatca agagcatctt gatagggaag aatcaattgc
    10801 accccttcaa gatgttctgt caataaattt agagacatct tctcagacat ttgagccgtt
    10861 gacaacttca agagattctt ataaccaact gtatttatcg caatacagta aacttctatt
    10921 gatctatttt caaccactat atcaaagcta aatccaacta ttggtcttag gtttgccttt
    10981 tgcgcctctt gaataaaatg atatgctgca taaagattac tgtggtccaa gataccaata
    11041 gtttgatatc ctaatgattt cgatttagag acatatgtct ttaaatcaac caagctatcc
    11101 ataaaagaat aaacggttct tgtatcaagt tgtgcaaaca tagtagcccc tttttacgat
    11161 tttttactat tatactatga aacaataaac gattaaagtc tcaatgataa ttccttgaca
    11221 tttcaaatat ttttttgtac tatttttata gtacaaaaag tacaagatta tgaaaggagt
    11281 tatctatggc ttgggaattc aatgaaaaat cacctattta ttcacaaatt gctgaacata
    11341 taaagatgca aattgttagt caagaaataa aaagtggtga ccaattgcct accgtgagag
    11401 agttagctca agaagctggt gttaacccaa ataccatgca acgtgccttc actgaattgg
    11461 aacgtgaagg aatggtattc tcacaacgta cttccggacg ctttgttaca gaagacaact
    11521 tactaattgg taaaattcgt caacaagttg caaaagcaga gttagcaact tttgtcaaca
    11581 acatgaaaaa aataggatat aaattagatg aaattacggt tgctctcgat cattttataa
    11641 aggagattaa ttaaatgaca cagttattac aacttcatca tgttacaaag aaatatcaca
    11701 aacatattgc tgttaatgat gtgactgtaa gcattccaac aggaaagatt ataggtttat
    11761 taggtccaaa tggtagcgga aaaacaacaa tcatcaaaat gataaatgga ttacttcagc
    11821 cagataaggg agatattgtt atcgatggtt acagaccgtc tgttgaaacc aaaaaaatta
    11881 tttcatatct ccctgatact tcatatttac aagaaaatat gaaaatcaaa gacgtcgtta
    11941 cattatttga agatttctat aacgatttta attcaaaggt agcttatcaa ctatttgagg
    12001 atcttaacct aaatcctaag gaacgtctaa aaaacctatc aaaagggaac aaggagaaag
    12061 ttcaactaat acttgttatg agtcgtaaag ctaggctcta catccttgat gaacctattg
    12121 gtggtgtaga ccctgcagcg cgtgactaca ttttaaaaac aatcattagc aactactcta
    12181 atgatgcttc tgttttaatt tcgactcact taatttctga cattgaacct atccttgatg
    12241 aagttatctt ccttaaagaa ggagaaattg acctacaagg caatgctgat gacttaagag
    12301 aagagcacaa ctgttcgatt gatgctctct ttcgtgaaag attcaagaaa taaaggagat
    12361 tgatatgttt ggaaaattat taaaatatga attaaaatca gttggtaagt ggtatctaac
    12421 actcaacgct gcggttttat tagtatctat tattttagga ctggtattaa aggcacttgg
    12481 cggcaatttt tctactgata ctaatagtac tagcgcccaa atattcacta taatactcgt
    12541 tttattgtta gcgatggtta tttcaggatc tctgttatcc acccttgcta ttattatcaa
    12601 acgtttttat agcaatatct tcggccgtca aggatatcta accttaacgc tcccagtaac
    12661 tacgaatcag attatttgtt caaagctact agcttcgctt ctttggagta tttttaatat
    12721 ttttattgtc attattggaa tcatattggt catattacct ttagtaggta ttggtcagtt
    12781 cgttgtcgct tttccagaaa tctataaaat tatttcatct tcgaacgctc cactttttat
    12841 cgcctatttc tttctatcgt acgttgcagg gacattactg atttatctct ctatcgctgt
    12901 agggcaatta tttacaaaca aacgtgtatt gatgggaatt gtttcttatt ttggaatatc
    12961 actcttaatt actttcttaa cattaataat agatagtatt tttcatattg atttattcaa
    13021 ctctcatgca aatgcaacat tctctcaacc tgtattgctc tacaatatac ttgttagcat
    13081 tgttgaaatt gctatattct atatgttgac acactctatt attaagtaca aacttaatat
    13141 acaataaaaa gattggagat atatctccaa ccttttttta tttttaattc aaaaaatttt
    13201 tcaaaatatc accgccataa atccacaaat agctataacc gatgatagaa ataggtttcg
    13261 ttatcataat gattgtcaca aagcgcttaa tagacatatt agatagaccg gtaatcatca
    13321 ccatgatgtc cgctggtgaa atgggagatg ccatacaaaa gataaaaaac ttttcatatc
    13381 cggatgtttc tagcttagac tcatatttat caaatgtctt ctgatccata aatagcaaaa
    13441 cgaattttcg tccataaaat ttgactaacc aaaaaaggat gacacttccg atgataatcc
    13501 caatatagtt ataaataaag cctaaggtag gaccaaaaat taagaaacct gctactgttg
    13561 taacacctcc aggaattact ggaaaaacaa tctgtacaat ttgagccaca ataaaaatca
    13621 ttggacccca aaattcatac ttgtgtacta ggtcttttaa ttcattgcta tcattcaaaa
    13681 taccaagttt gtacaaataa acaactaaga caagggtcgc tattaacgct aagacaccca
    13741 aagccttaat gaccttctgc caaaagcggt aacgttttga taatttcata ttcatgcttt
    13801 aatcataaca caactgttaa atttttgcta ttttaaatag tatttgttat actatattgg
    13861 taagttttta tgctttattc tacttttggg gtcgttacgg attcgacagg cattatgagg
    13921 tatattttgc gactcatcgg cagatgtaaa atgccagtta aatataactg caaaaaatac
    13981 aaattcttac gcattagctg cctaaaaaac agcctgcgtg atcttcacaa gattgtttgc
    14041 gttttgctag aaggtcttat ttatcagcaa actacgtttg gctactgtct agttagttaa
    14101 aaagagattt atagactcgc tatgtgaggg cttgagttat gtgtcatcac ctagttaaat
    14161 caatacataa cctatagttg tagacaaata tattagcaga tgtttggacg tgggttcgac
    14221 tcccaccggc tccatatcat tttatcataa cttactatac atacttaaaa ccttgttatt
    14281 acaaggtttt tattttgttt ctttgctcat acattgcccc tatcttagga aaaaaatact
    14341 tggaacagac tctaacattg aattaggcca cctcattagc tcactctttg tcaatatccg
    14401 agaataagtt ctttatcatt ctaatttata gaatttatta aaaaagataa ggtttttaga
    14461 ggaaaaaatg ttataatcta tttagcctac gattggaggc tagttatggc tagtatttta
    14521 aaaaaacatt tagagaaacg tttagagctt aaaaatgatc ctatgaggac agatatgagt
    14581 actggtgcca gttaccctaa atatggattc tcggtaggaa agtacacata tggatatcaa
    14641 caattctttt acgaaggcgt taacttaaaa gaaattggag cattttgctc aatagcccag
    14701 aacgttacta ttactggtat gaatcatcca accgatcaca ttaccactaa cccttttatt
    14761 tactataagt caaggggatt tataaatgag gatagggcgg acttaataga tgaaaagaaa
    14821 aatggaaaag ttattattgg caacgatgtc tggataggaa ctaatgtcac tatacttcca
    14881 tctgttacaa taggtaatgg agcaattatt gaagcaggtt cagttattac caaagacatt
    14941 cctgactatg ctgttgtcgc tggaacacct gctaaaatta tcaaatatcg cttttcagag
    15001 gaagaaataa cattattaaa tgcatctcag tggtggaatt ggtcagatga agctattaag
    15061 gaacatatta gcgaattttc agataagagg gaattcttca atactctaaa aagtatttct
    15121 gaaaacaaaa atcataaact ctagaatgac ttcatcaaat agcacactaa aatactactc
    15181 agcataatat gagtagtatt ttagtatgct attttgctag tttatttagc acatgtttag
    15241 actaatgcaa ttctaggaaa gctacttagt ctctctgcta ttatcatgat aagctcccca
    15301 aaatatggtt actaataatt ttaaagattt gttaaataac ctttttttat catcatctag
    15361 ttctctaaag ctatgaaaag ctttcatcat ttttgctctg gaagccaagt tgaagtcatc
    15421 caaactagat acgccacttg ataggaagct atcaaataat aaatcaaaaa ataattttct
    15481 ttcttgactt tttaattcag ccatccactg ttgaaatgtc ttttcaaaac aaatactttt
    15541 tagcgatagt cctgctccag tctttaaaat agctggttct tgaatttccc aagttgtaac
    15601 atcgtgttgc agaattccac gcgcattgct actaataatt agcggctcta tatcactata
    15661 aagcatcata ccaacaatag attcttctgg tagtaagcga atgcactttt gtttagtagt
    15721 tttatactct gtagtaggta acaaagtttt ttgtagtcct ggggagtcaa taagccaaat
    15781 caaatcaatt tttgctttta aatctggctt tgtaaacatt gcagcgtaca aggctaaatt
    15841 accaccctta gaatgacctg agagaactac tttatcaaaa taaggcagta ttgtatttaa
    15901 gtatttaatc gctgaacgat gagccgatat ttctctcata tacgtcaatt taaaatcttc
    15961 tttccaacca ataaggttgg catcagtacc acgaaataca acctgatgat aatcaatatt
    16021 aggaatagta aaaaccatag ctgcaaattg tttttcaaat tctaaactaa tctcagcttg
    16081 ataataagat aaagttaagt ttttaaaacg tttcgaggtc atcattaact gacataaatc
    16141 atgtcgttgt gacgtgaa
//
LOCUS       DATRUK010000048        16045 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.048, whole genome shotgun sequence.
ACCESSION   DATRUK010000048 DATRUK010000000
VERSION     DATRUK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16045
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.048"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            353..1315
                     /locus_tag="VBE34_001433"
     CDS             353..1315
                     /locus_tag="VBE34_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7264145.1"
                     /translation="MKKITKMVISSVLASVLMTACSQSSQNGQSDVKHIGILQYVEHP
                     SLTATRKGFIKELAKEGYKDGKNIKIEYKNAQGDQSNIQSISEKLIKDNKLVLGIATP
                     AAQSLTTVSTETPILFTAVTDPVSAELVKSMKKPEGLATGTSDMSPIKKQVSLLRKVM
                     PKVKRVGIMYTTSERNSEVQVKQAKKIFQEAGIKTSVKGISSTNDVQDTAKSLMSKTE
                     VLFVPTDNIIASSVTLLGNLSKELKVPVVGGSADMVPSGLLFSYGADYEALGCQTARQ
                     AVKILKGKDVAKVPSEYPQNLKVVVNEDMAKELGIDVSSIKNNK"
     gene            1331..2227
                     /locus_tag="VBE34_001434"
     CDS             1331..2227
                     /locus_tag="VBE34_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7264146.1"
                     /translation="MLDIILSGISQGLLWSIMAIGVFITFRILDIADLSAEGAFPMGA
                     AVCALCIVNDINPIVATIAGMLGGMLAGLVSGFLHTKMKIPALLTGIITLTGLYSINL
                     LVLGRSNVSFALKNTLVTMVTRLGLNKLSAVLLIGIVCVGLVILILYLFLNTQLGLAL
                     RATGDNEAMGQANSIKVDRMKMLGYMIGNGLIALSGALLAQNNGYADLNMGVGTIVIG
                     LASIILAEVMIKYLPLGKRLWSIVLGSVLYRMIIVFILTTDIDAQMIKLVSAILLALI
                     LYVPELRAKLGVKASKTLTKEG"
     gene            2232..3035
                     /locus_tag="VBE34_001435"
     CDS             2232..3035
                     /locus_tag="VBE34_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7264147.1"
                     /translation="MSLLELVNLHKTFEKGTVNENHVLRGLDLTIEDGDFISVIGGNG
                     AGKSTLLNCLAGLIPIDQGTITLDNQSITKDSVEKRSKGISRVFQDPRMGTATNLTIE
                     ENMAIAHKRGNKRHIFRQSVTDDDRQLFKKSLSQLGLGLENRMKTDAAFLSGGQRQAL
                     TLAMATLVRPKLLLLDEHTAALDPKTSDMVMELTQKVIEEQRLTALMITHNMEHAIAY
                     GNRLVMLYHGKIVVDVKGEAKRNLTVAELMELFHKNSGQQLIDDALVLG"
     gene            3210..3494
                     /gene="groES"
                     /locus_tag="VBE34_001436"
     CDS             3210..3494
                     /gene="groES"
                     /locus_tag="VBE34_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEO7264148.1"
                     /translation="MLKPLGDRVIISFVETEEKSVGGFVLAGASHDTTKTAKVLAVGD
                     GIRTLTGELVAPSVAEGDTVLVENGAGLEVKDGNEKVTVVRESDIVAVVK"
     gene            3590..5212
                     /gene="groL"
                     /locus_tag="VBE34_001437"
     CDS             3590..5212
                     /gene="groL"
                     /locus_tag="VBE34_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEO7264149.1"
                     /translation="MAKDIKFSADARSAMVRGVDILADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPIGIRRGIETAVSAAVEELKEIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVSELENPYI
                     LITDKKISNIQEILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVVTEDLGLDLKDATMQVLGQSAKVTVDKDSTVIVEGA
                     GDSSAIANRVAIIKSQMEATTSDFDREKLQERLAKLAGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVSGGGTALVNVIEKVAALKLNGDEETGRNIVLRALEEPVRQ
                     IAYNAGYEGSVIIERLKQSEIGTGFNAANGEWVDMVTTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPEAPTAPAMDPSMMGGF"
     gene            complement(5244..5608)
                     /locus_tag="VBE34_001438"
                     /pseudo
     CDS             complement(5244..5608)
                     /locus_tag="VBE34_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5621..6358)
                     /locus_tag="VBE34_001439"
     CDS             complement(5621..6358)
                     /locus_tag="VBE34_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7264150.1"
                     /translation="MPKNELNNKLNKLKHVQVYNKIFNMIQDGTYSPGMQLPSEPELA
                     GQLNVSRATLRKSLALLQEDHLVKNIRGKGNFIRENSSNLSENGYENRQHPIKTCLTS
                     KITEVELEFRVEVPAEAITASLKQETPVVVIADRWYHTDDGPLAYTLSFIPIELISDA
                     EISLHDTKQLLNFIEEGIYHEGISSHSQSHLGYATSGNFSATKYTLSDHGQFILIQET
                     IFNQEKILMCNKHYVPIEHFELSITSH"
     gene            6517..7296
                     /gene="udp"
                     /locus_tag="VBE34_001440"
     CDS             6517..7296
                     /gene="udp"
                     /locus_tag="VBE34_001440"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEO7264151.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            7317..8519
                     /locus_tag="VBE34_001441"
     CDS             7317..8519
                     /locus_tag="VBE34_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEO7264152.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQVVSVVSTGVTKVINCGQAGLNFVFGSLADSGTKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILFPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRGTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            8549..9220
                     /gene="deoC"
                     /locus_tag="VBE34_001442"
     CDS             8549..9220
                     /gene="deoC"
                     /locus_tag="VBE34_001442"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEO7264153.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIKNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVDEGVKIKAAGGISSLEDAEKFIALGASRLGTSRIIKIVKNQKV
                     EEGTY"
     gene            9287..10498
                     /locus_tag="VBE34_001443"
     CDS             9287..10498
                     /locus_tag="VBE34_001443"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO7264154.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGDPGNFTRT
                     ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHDMGHNKSNSHGIDTL
                     IKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPEIISAMRDKDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADISATVADNFGVDTA
                     MIGESFLQDLV"
     gene            10668..12308
                     /locus_tag="VBE34_001444"
     CDS             10668..12308
                     /locus_tag="VBE34_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264155.1"
                     /translation="MFDFFKKKVVKVCLVIFGIVLVSLLSLGFFYFSKGQVLSRFVAA
                     RSRTSGQAFDNIKEYMVWSDTGESITNDEANYANFEPLSKSEARKLGQEIKEGNKNDS
                     MYLKRVGSRLGIFPDYRIANKPMSLTLKTNVPKLDVLLNQKKVATSNSDHFSVTVERL
                     PRTHYTASLEGTSDGKEIKLKKDYDGKNQTIDLSVAFKSFTVTSNLMDGNLYFGDNRI
                     AKLKDGSYSVENYPVTDGSKAYIKKVFNDGEITSHKQKLISIADNQTIKLDVDGLLNE
                     KEAGQKLITAFNQLILYVSTGQDPQTLGTVFEKGAENDFYKGLKESIKAKFVTDNRKA
                     SHFTIPNIVLNKMTQVGKESYQVNFAADYDFNYDKSTDPDKKTYGHIIQNLTGNFIMK
                     KSGNSYLISNDGKKDITVAKETNKVKADPVSIFPENLVGSWKGEVEDGTVTMTFDKDG
                     KVTQKKVYKDSKSKESNHSAKVTKLEDKGNGLYLYQYESGTDTTTFVTGGIGGLKVKY
                     AYGIKIEGNKIIPVIWQTSSDGEFDYHKPLLSKPLTKQ"
     gene            complement(12333..13217)
                     /locus_tag="VBE34_001445"
     CDS             complement(12333..13217)
                     /locus_tag="VBE34_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO7264156.1"
                     /translation="MSFSLKIRNPYGEHTVKELLEDYFLIPRKIRHFLRVKKHVLINN
                     EFINWQTVVQENDTITLIFDDEDYPTKKIPLGRAELIDCLYEDEHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSAYSGQTCYVVHRLDMETSGAVLFAKNPFILPLINQRLERKEIWR
                     EYWALVEGKFSPKHQVLRDKIGRNRHDRRKRIIDSKNGQHAMTIIDVLKYIQNSSLIK
                     CRLETGRTHQIRIHLSHHGHPLIGDPLYNPSSNNERLMLHAHRLTLSHPLTCETISVE
                     APSSTFEKVLNNYKKGVG"
     gene            13261..15582
                     /locus_tag="VBE34_001446"
     CDS             13261..15582
                     /locus_tag="VBE34_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7264157.1"
                     /translation="MKLFDKFIDLFRVDEDNDEMTRKNEQETREETSNLDGEEVYDID
                     DITRPSKSQYQRGIRHQKENAKSRLEWLQKVDRYLPSPKNPIRRFWRRYRIGKLLFIA
                     LMAFILIFGSYLFYLSKTATVSDLQSALKTTTTIYDKNKEYAGKLSGQKGTYVELNAI
                     SDHLKNAVIATEDRTFYENNGVNFKRFFLAVATLGKFGGGSTITQQLAKNAYLSQDQT
                     IKRKAREFFLALELTKKYSKAEILTMYLNNSYFGNGVWGVEDASRKYFGTSAANLTVD
                     EAATLAGMLKGPEVYNPYYSVENATNRRDTVLAAMVDAGQLTKSQAKEAASIGMKNRL
                     ADTYAGKINDYKYPSYFDAVVNEAIDTYGISEKDIVNNGYKIYTALDQNYQSGMQKTF
                     DDTSLFPVSDYDGQSAQGASVALDPKTGGVRGLVGRVQSTKDAQFRSFNYATQSKRSP
                     ASTIKPLVVYSPAIASGWSIDKELPNKVQDFHGYKPSNYGGIETESIPMYQALANSYN
                     IPAVYTLDKLGINKAFTYGRKFGLNMSSANKELGVALGGSVTTNPLEMAQAYSTFAND
                     GIMHRAHLITRIETANGKLVKQFTDKPKRVISRSVASKMTSMMLGTFSNGTAINANVY
                     GYTMAGKTGTTETDFNPNLSGDQWVVGYTPDVVISQWVGFKNTDKHHYLTDSSAGTAS
                     NIFSTQASYILPYTKGSSFTHIENAYFQNGIGSVYNAQDASNTTNQESRSIINDLKDS
                     ASKAAQDISRAVEDSNFQEKVKDAWNSLKDYFR"
     gene            15631..15783
                     /gene="rpmG"
                     /locus_tag="VBE34_001447"
     CDS             15631..15783
                     /gene="rpmG"
                     /locus_tag="VBE34_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO7264158.1"
                     /translation="MAQKKASLACTECGSRNYSIGVSSTPKPTRLEVNKFCKYCKKYT
                     IHKETR"
     gene            15819..15992
                     /gene="secE"
                     /locus_tag="VBE34_001448"
     CDS             15819..15992
                     /gene="secE"
                     /locus_tag="VBE34_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEO7264159.1"
                     /translation="MFVKGIFQVLRDTTWPNRKQRWKDFISILEYTVFFTIVIYIFDK
                     LLAAGVMDLINRF"
BASE COUNT         5183 a         2539 c         3327 g         4996 t
ORIGIN      
        1 atttttaaat gacgtaaact tataggcaac cataaacaag gagagaataa tatgttaatc
       61 aaatcgtcta ttattaaaca aacaaatact tattattact ttagtttctt tagatagacg
      121 tttgtagcct caaggataca aacgtcacgc aaagtttgta tctatgaagc taaagcattt
      181 tatgatgaaa gcagagccga ttagatcatt tcaataatct agttggttaa ggcaatattt
      241 tttcgccctc ccgtgcacag aggaattgag agaaataatt aaccaactag atgtagggtc
      301 tagttggttt tttgttttat ggaaaatttg atggtattac aaggagatag taatgaaaaa
      361 aataacaaag atggtaatta gcagcgtttt agcatctgtt ctgatgacag cttgttcaca
      421 atcttctcaa aatggtcaat ctgatgtaaa acatattggt attttacaat atgtagaaca
      481 tccctctcta actgccacta ggaaaggatt tataaaagaa ttagctaaag aaggctataa
      541 agatggcaaa aatatcaaaa ttgagtataa gaatgctcaa ggggatcagt caaatatcca
      601 atcaatttct gaaaaactca ttaaagataa taaattagta ttgggaattg cgactccggc
      661 ggctcaatcg ttgacaacgg tgtcaacaga aacacctatt ttatttacag cagtgacaga
      721 tccggtatca gccgaattag taaaatcaat gaaaaaacct gaaggtttag cgactggtac
      781 aagtgacatg tctccaatta aaaagcaagt atcccttttg agaaaagtaa tgcctaaggt
      841 taaacgtgtt ggtattatgt ataccactag cgagcgtaac tcagaagttc aggtaaaaca
      901 agcaaaaaaa atcttccaag aggcggggat taaaacaagt gttaaaggta tttcttccac
      961 taatgatgta caagatactg ctaagagttt aatgtctaaa acagaggttc tttttgtacc
     1021 aacagataat attatagcca gctcagtaac gctgttaggg aatctatcga aagaattaaa
     1081 agtgcctgtt gtgggaggtt cagcagatat ggtaccaagt ggtttgctct tttcttatgg
     1141 ggctgattat gaggctttag gttgtcaaac ggcaagacaa gctgttaaaa tcctcaaagg
     1201 taaagacgtc gctaaagttc cttcagaata tcctcaaaat ctaaaagtag tagttaatga
     1261 agatatggca aaagagcttg gaattgatgt ttcaagtatt aaaaataata aataatcaga
     1321 aaggtattgt atgttagata ttatcttatc cggaatttcg caaggattac tttggtcaat
     1381 tatggcaatt ggcgtgttta tcacttttcg tatcttagac atagccgatc tctctgcaga
     1441 aggggctttc cctatggggg ctgcagtttg cgccttatgt atcgttaatg atattaatcc
     1501 aattgtggca accatagcgg gcatgttagg tggtatgttg gcaggactag tatcgggttt
     1561 tcttcatact aaaatgaaaa taccagccct cctaacaggt attattacgt tgacaggact
     1621 ttactctatt aatttacttg ttttaggaag atcaaacgtc tcttttgctc tcaaaaatac
     1681 tttggttaca atggtaacaa gattaggttt aaataaactt tcagcagttt tattaattgg
     1741 aattgtttgt gtaggtttag ttatcttaat tttgtatctc tttttaaata cccaactagg
     1801 attagcttta agagcgacag gtgataatga ggctatggga caagccaata gtattaaagt
     1861 agatcggatg aaaatgctag gatatatgat tgggaatggc ctgattgctt tatctggtgc
     1921 acttttagcg caaaataatg gctatgcaga tttgaatatg ggagtgggta caatcgttat
     1981 tggattggct tcaattatct tggcagaagt aatgataaaa tacttacctc ttggtaaaag
     2041 gttatggtca attgtattag gttctgtttt ataccgtatg attattgttt tcatattaac
     2101 aaccgatatt gatgcacaga tgataaagtt ggtttcagca attttgttgg ctctcatttt
     2161 gtatgtccca gaattacgag ctaaactagg agtaaaagcc tctaaaacgc ttacaaagga
     2221 aggataagaa catgtcatta cttgaattag tcaatctgca caaaactttt gaaaaaggaa
     2281 ctgtcaatga gaaccatgtt ttacgtggtt tagatttaac gattgaagac ggtgatttta
     2341 tttcagtaat tggaggtaac ggtgcgggaa aatcaaccct ccttaactgt ctcgctggtc
     2401 tgatccctat tgatcagggg acaatcacat tagacaacca atcaattact aaagactctg
     2461 tagaaaaacg atctaagggt attagtcgag tctttcagga tcctagaatg ggaactgcaa
     2521 caaatttgac gattgaagaa aatatggcaa ttgcacataa aagaggcaat aagcgtcaca
     2581 tttttagaca gtcagtgact gacgatgacc gtcaactatt taaaaagtct ctatcacaat
     2641 taggtctagg cttagaaaat cgaatgaaga ctgatgctgc atttttatct ggcggtcagc
     2701 gtcaagccct cacgttagct atggcaactc ttgttcgtcc caaattatta ttactggatg
     2761 aacatacagc ggctctagat cccaaaacta gcgatatggt tatggaatta acacagaaag
     2821 tgatagaaga acaacgattg acagccttaa tgattacaca caatatggag catgccattg
     2881 cttatggtaa tcgtctcgtg atgctctacc acgggaaaat tgttgttgat gttaaaggag
     2941 aagcaaagag gaacttaaca gttgcagaac taatggaact tttccataaa aactcaggtc
     3001 aacagttgat agatgatgcc cttgtcttag gttaaaatat aaatttctct agtgaatgga
     3061 caggataact agggaaattt ttcttgtttg aaacgtgcct tttctcttga ctaattctga
     3121 ccaagtgata gaatagtttt tgtagttagc actctcttta taggagtgct aaaaaatcaa
     3181 aatatatctc gtatggaggt tcgtttatta tgttaaaacc attaggtgat cgtgtgatca
     3241 tttcgtttgt tgaaactgaa gagaaatctg tcggtggttt tgttcttgca ggagcaagcc
     3301 atgatacgac aaagacagcc aaagttcttg ctgtgggtga cggtattcgt accttaacag
     3361 gggaattagt tgcacctagc gttgcagagg gtgatactgt tttagtcgaa aatggtgctg
     3421 gtttggaagt taaagacggt aacgaaaaag tgacagttgt tcgcgaaagt gacattgttg
     3481 ctgtcgttaa ataggtttta ttaagcaaga aaggatatag gcctattaga gaaaagttgc
     3541 tttgtaaaga aagtagactt tgttaaattt taggcctggg tattaaatta tggcaaaaga
     3601 tattaaattt tcagcagatg cccgctcagc aatggtgcgt ggtgttgata ttttagctga
     3661 tacagtcaaa gtaacattag gtcctaaagg ccgtaatgtt gttcttgaaa aagcatttgg
     3721 ttctccttta attacaaatg atggtgtgac aattgctaaa gaaattgagc tagaagatca
     3781 ctttgaaaat atgggagcta aacttgtgtc agaagtggct tcaaaaacta atgatattgc
     3841 aggggatggc actacaactg ctactgtttt gacccaagct attgtacggg aaggtcttaa
     3901 aaatgtaact gcaggggcaa atccgattgg cattcgtcgt ggtattgaaa cagctgtttc
     3961 agcagcagtt gaagagctaa aagagattgc acaaccagtt tcaggcaaag aagctattgc
     4021 tcaagttgcg gctgtgtctt cacgttctga aaaagttggg gaatacattt ctgaagctat
     4081 ggagcgcgtg ggtaatgatg gtgttatcac tattgaagaa tcgcgaggta tggaaacaga
     4141 gcttgaagtt gtggaaggaa tgcagtttga ccgtgggtac ttgtcacagt atatggtaac
     4201 tgataacgag aaaatggtct ctgaacttga gaatccgtat atccttatta cagataagaa
     4261 aatttcaaat atccaagaaa ttttaccatt attagaagag gttcttaaaa caaatcgtcc
     4321 gttgctaatc atcgctgatg atgttgatgg agaagctctc ccaacgcttg ttcttaacaa
     4381 aattcgtgga actttcaatg tcgtagctgt taaagcgcct ggatttggtg atcgtcgtaa
     4441 agccatgctg gaagatattg ctatcctaac aggaggaact gtcgttactg aagaccttgg
     4501 tttagactta aaagatgcta ctatgcaagt tttaggacag tctgctaaag taacagtaga
     4561 taaagattct actgttattg tcgaaggtgc cggtgactca tcagcaattg ctaatcgcgt
     4621 agccatcatt aagtcacaga tggaggctac aacttctgat tttgatcgtg aaaaattaca
     4681 agaacgactt gctaagttag ccggtggtgt agcagtaatt aaagttggtg cggcgactga
     4741 aacagaatta aaagagatga aacttcgtat cgaagatgcg ttaaatgcaa cgcgtgctgc
     4801 agttgaagaa ggtattgttt caggtggagg tacggctctt gtgaacgtta ttgaaaaagt
     4861 agcggcactg aaacttaatg gtgatgagga gactggacgt aatattgttc ttcgtgctct
     4921 cgaagagcct gttcgtcaaa ttgcttacaa tgctggatat gaaggttcag ttattattga
     4981 acgtttaaaa cagtctgaaa ttggtacagg atttaatgcg gctaatggag aatgggtaga
     5041 tatggttacc acaggtatca ttgatcctgt caaagtaaca cgttctgcac ttcaaaatgc
     5101 ggcatctgta gcaagtctta tcttgactac agaagcagta gtagcaaata aacctgaacc
     5161 agaagctcct acagctcctg caatggatcc atctatgatg ggtggcttct aatcactaac
     5221 ttatcacaac ataaaactcc cctttaacaa aggggagttt tatgttgtga taatagagat
     5281 atgatagtca ctaccacttg cggtaagaag atcataagga taggccatct aatatttgcc
     5341 agtagtggga ggaaaaaagt agcaaaacaa tgattgctat tatcttagat tgagattgat
     5401 aagctaagaa ttgaattata gctaatagaa ttaagtaact aataagtata aataacatag
     5461 aaaagagcat tccgtaccca gcacccattg acggttcctt tggaggggct agaaaataac
     5521 ttaacttaaa ataagcgcca actattaggc ccataattaa ttcggtaaaa gttatgaaaa
     5581 aagcaacttt taatagtcta ttttgcatag gcttgcctcc ttaatgagag gtaatggaca
     5641 attcaaagtg ctcaatggga acgtaatgct tattacacat tagtattttc tcttggttaa
     5701 aaattgtttc ttgtattaat atgaattgtc catgatcgga tagggtgtat ttagttgctg
     5761 aaaaattgcc agatgtcgca tatcccaaat ggctttgtga gtggcttgat atgccttcgt
     5821 gatagatacc ttcttcaatg aagtttaaca actgtttagt atcatgtaaa gaaatctcag
     5881 catctgaaat aagttcgata gggataaaag aaagtgtgta agctaatggt ccatcatcgg
     5941 tgtggtacca acgatcagca ataacaacta caggggtttc ttgtttaagt gaagctgtga
     6001 tagcttctgc aggaacctct acacgaaatt ctaattccac ttctgtaatt ttggaagtta
     6061 gacaagtttt aatggggtgt tgtctatttt cgtaaccgtt ttcagacaag ttgctagaat
     6121 tttctctaat gaaattacct tttcctctga tatttttaac caaatggtct tcttgtaata
     6181 gtgctaatga ctttcgtaaa gtagctcgac taacattaag ttgccctgct aattcgggct
     6241 cagaaggcaa ttgcattcca ggagaataag taccgtcttg aatcatgtta aaaatcttat
     6301 tgtagacttg cacatgcttt agtttattta gtttattatt tagttcattt ttaggcattg
     6361 taacctccgt atacaagtta gtttaattga aaaaattaga aaaatcaagt taaaaaataa
     6421 aacgttttct ttcgataaaa gcgtttacaa aggttgtctt gtatgttatt atacaagtgt
     6481 aaaaaaaata gacaagacat taaggaggtc tttatcatgc agaattattc aggtgaagtc
     6541 ggattgcaat atcacctaca gattcgtcca ggtgatgtag gtcgttatgt tatcatgcca
     6601 ggtgatccaa aacgttgtgc aaaaattgca gaacattttg ataatgcagt tcttgtagca
     6661 gacagccgtg aatatgttac ttatacaggt acattaaatg gtgaaaaagt cagtgttact
     6721 tcaactggta ttggtggtcc atcagcgtct attgcgatgg aagaattgaa actctgtggc
     6781 gctgatacct ttatccgtgt tggaacatgt ggtggtattg accttgatgt taaaggcgga
     6841 gacatcgtta ttgcaactgg agctatccgt atggaaggta caagtaaaga atatgctccg
     6901 attgaatttc ctgctgtagc tgacttggag gtaacgaatg ctctagttaa tgcagctaaa
     6961 aagcttggtt acacaagtca cgcaggagta gttcaatgta aagatgcatt ttatggacaa
     7021 catgagccag aacgtatgcc agttagttat gaattattaa ataaatggga agcatggaaa
     7081 cgtctgggaa caaaagcgtc tgaaatggaa tctgcagctc tttttgtagc agcaagtcat
     7141 ctaggagttc gttgtggatc agatttcctt gttgttggta atcaagaacg caacgctttg
     7201 ggtatggata acccaatggc tcatgacaca gaagcagcta ttcaagttgc tgttgaagca
     7261 ctccgcacac ttattgaaaa tgataaatca caataagcga ggttttggaa gtcatcatgc
     7321 aatttattta tagtattatt ggtattttat tggtattagg aattgtgtat gcaatttctt
     7381 tcaatcgtaa gagtgtttct ctaagtttaa ttggaaaagc tcttatcgtt caattcatta
     7441 ttgcgctaat cttagtacgt atcccactag gccaacaagt tgttagtgtt gtttcaactg
     7501 gagttactaa agtaatcaac tgtggtcaag ctggtttaaa ttttgtgttt ggttcattag
     7561 cagatagtgg aacaaaaact ggttttattt tcgccattca aacgcttggt aatattgttt
     7621 tcttatctgc cctagttagt ctactttatt atgtaggaat ccttggattt gtagtaaaat
     7681 ggataggtaa gggtgttggt aaaattatga aatcctcaga ggttgagagt tttgttgccg
     7741 tagctaatat gtttcttggt caaacagaca gtccaatttt ggttagcaaa tacctaggtc
     7801 gtatgactga tagtgagata atggttgtgt tggtatcagg tatgggaagt atgtcagttt
     7861 ctattcttgg tggctacatt gcattaggca ttccaatgga atatctcttg attgcttcaa
     7921 caatggttcc tattggcagt attctcattg ctaaaatctt atttcctcaa acagaacctg
     7981 ttcaaaaaat tgatgacatt aagatggata ataaaggtaa taacgccaat gtgattgatg
     8041 caatcgctga gggtgcaagc acaggtgcac aaatggcttt ctcaattggt gctagtttga
     8101 ttgcctttgt tggtttagtt tctttgatta atatgatgtt aagtggattg ggaattcgct
     8161 tagaacaaat cttctcatat gtttttgctc catttggttt tcttatggga tttgaccaca
     8221 aaaacattct tctagaagga aaccttcttg gaagtaagtt gattttaaat gagtttgttt
     8281 cgttccaaca attgggtcac ctaatcaaat ctttagatta tcgtacagca ttggtagcaa
     8341 ctatttcact ctgtggtttt gctaatttat caagtttagg tatttgtgtt tcaggtattg
     8401 ctgttctttg tccagagaaa cgtggcaccc tagctcgact tgttttccgt gcaatgattg
     8461 gtggtattgc tgtaagtatg cttagcgcct ttatcgtcgg tattgtaact ctattctaaa
     8521 agttgacaaa agaaagaagg atagtaacgt ggaagtaaaa gatattttaa aaacggtaga
     8581 ccatactttg ctagcaacaa cagcaacatg gccagaaatc caaacaattt tagatgatgc
     8641 aatggcttat gaaacagctt cagcatgtat tccagcctct tacgtcaaaa aagcagcaga
     8701 gtacgtttca ggtaaattag ctatttgtac tgttattggg ttcccaaatg gctatagtac
     8761 aactgcagcg aaggtttttg aatgtcaaga tgctattaaa aatggtgctg atgaaattga
     8821 catggtcatt aatttgacag acgttaaaaa tggggatttt gatactgttg aagaagaaat
     8881 tcgtcaaatc aaagctgcat gtcaagacca tatcttaaaa gttatcgttg agacatgtca
     8941 attaactaaa gaagaactta tcgaactttg tggagttgtc acacgttcag gtgcagactt
     9001 tattaaaacc tctactggtt tttcgacagc aggtgctaca tttgaagatg ttgaagtgat
     9061 ggcaaaatat gtcgacgaag gtgttaaaat taaggcagca ggtggaatct catcattgga
     9121 agatgctgaa aaatttattg ctttaggagc ttcacgcttg ggtactagcc gtatcatcaa
     9181 gattgttaag aaccaaaaag ttgaagaagg cacttattaa ataatataaa ggaaatagga
     9241 aggatgctgg tctgacttcc tattttcaat tgataaagga gaagttatgt cacaatttga
     9301 tcgcattcac ttagttgtct tagattcagt gggtattgga gcggcaccag atgccaatga
     9361 ttttgtcaat gcaggagttc ctgatggtgc ttcagacacg cttggccaca tttcaaaaac
     9421 agttggactt gcggtgccaa atatggctaa aattggactt ggtaatattc ctcgtcccca
     9481 agcgcttaaa accgttcctg cagaagaaaa tccaagtggc tacgcgacga agttacaaga
     9541 agtatcactt ggtaaagata caatgacagg gcattgggag atcatgggct taaatattac
     9601 agagccattt gatactttct ggaatggttt tccagaagat atcatcacaa agattgagga
     9661 tttttcagga cgtaaagtta ttcgtgaagc gaataaacct tattcaggga cagctgttat
     9721 tgatgacttc ggaccacgcc aaatggaaac tggtgagctc atcatctata cctcagctga
     9781 cccagtccta caaattgcag ctcatgagga tatcatccct ctagaagaac tttaccgtat
     9841 ttgtgaatat gctcgtagta tcacaatgga gcgtccagct ctccttggtc gtattatcgc
     9901 tcgtccatat gtaggtgacc caggcaactt tacgcgtaca gccaaccgtc acgactatgc
     9961 agtatcacca tttgaagata ccgtattaaa taaactagat caagctggca tcgatactta
    10021 tgctgttggt aaaatcaatg atattttcaa tggttcaggc attaatcatg atatgggaca
    10081 taacaaatcg aatagtcatg gtattgatac tttgataaaa acaatggggt tatcagagtt
    10141 tgaaaaaggc ttctccttta ctaatttggt tgactttgat gctctctatg gtcaccgtcg
    10201 tgatcctcat ggctatcgtg attgtcttca tgagtttgat gagcgcctcc cagagatcat
    10261 ttcagcaatg cgtgacaaag acttactttt gatcactgcc gatcatggta atgacccaac
    10321 ttatgcaggt acagaccata caagagaata cattcctctc ttagcctata gcccatcttt
    10381 cacaggaaac ggccttatcc cagtagggca tttcgcagat atttcagcaa cagttgcgga
    10441 taactttggt gttgatactg cgatgattgg agagagcttc ttgcaggact tggtataatc
    10501 atagacaaaa attttagagg ttgaaatcgg attatggttt caatcctttt ttcgttttgt
    10561 tataatagaa aaatagcggt tacaagtaca aatacagctg ttttatcagt gttgtaactt
    10621 ttctttaagc aatttacttt aatataaagt gaatgagagg ataaggtatg tttgattttt
    10681 ttaagaaaaa agttgttaaa gtgtgcttag ttatttttgg tatagtttta gtatcacttc
    10741 tgagtctagg attcttttat ttttcaaagg gacaagtact tagtcgtttt gtagcagcac
    10801 gttctcgtac gtcaggtcaa gcgtttgata atattaaaga atacatggtt tggtcagata
    10861 cgggagaatc tatcacaaat gatgaggcca attacgctaa ttttgagcct ttgtctaaaa
    10921 gtgaggcgcg taaattaggg caggaaataa aagaaggaaa caaaaatgat tcaatgtatc
    10981 taaagcgtgt aggtagtcgc ttaggtatat tccctgatta tcgtattgct aataagccta
    11041 tgtctttgac cttgaaaacc aatgttccta aattggatgt tttgttgaat caaaaaaagg
    11101 tcgcgacaag caattcagat catttttcag taacggtgga acgtttacct agaacccatt
    11161 atactgctag ccttgaaggg actagtgacg gaaaagagat taaactcaaa aaagattatg
    11221 atggtaaaaa ccaaactatt gatttatcgg tcgcttttaa atcttttaca gtaacaagta
    11281 atcttatgga tggtaatctt tattttggtg ataatcgtat tgctaaatta aaagatggta
    11341 gctattccgt agagaattat ccagtgactg atggttcaaa agcttatatc aaaaaagttt
    11401 ttaatgatgg tgagataacc tctcataaac aaaaattaat ctctatcgct gataatcaga
    11461 caatcaaatt agatgttgat ggtttattaa atgaaaaaga agctggacag aaattgataa
    11521 ctgctttcaa tcaattgata ctttatgtat caactgggca agaccctcag actttaggaa
    11581 cagtttttga aaaaggtgcc gaaaatgatt tttacaaagg attgaaagaa agtattaaag
    11641 caaaatttgt aactgataat cgtaaagcga gccatttcac tattcctaat attgtcctta
    11701 ataaaatgac acaggttggg aaagaaagtt atcaagttaa ttttgctgcc gattatgatt
    11761 ttaattatga taaatcaaca gatcctgata agaaaacata tggccacatt attcagaatt
    11821 taactggtaa ttttattatg aaaaaatcgg gaaatagtta ccttatttct aatgatggta
    11881 aaaaagatat cacagtagct aaggaaacta ataaagttaa ggctgatccc gtttctattt
    11941 ttccagagaa ccttgtagga agttggaaag gtgaagttga ggacggtaca gtaacgatga
    12001 cttttgataa ggatggtaaa gttactcaga aaaaagttta caaggatagt aaatctaagg
    12061 aaagtaacca ttctgctaaa gttacgaagt tggaggataa gggaaatggg ctctaccttt
    12121 atcaatatga atcaggtact gatactacaa cttttgtaac aggaggtatc ggtggtctta
    12181 aagttaaata cgcctatgga ataaaaattg aagggaataa gatcatccca gtaatctggc
    12241 agacatctag tgatggagaa tttgattacc ataaaccact actcagtaag ccattaacta
    12301 agcaataatg attaaaagga gttggataat tattatccaa ctcctttttt ataattgttt
    12361 aaaaccttct cgaaagtaga tgaaggggcc tctacgctaa tagtttcgca agttaatgga
    12421 tgggatagag tcaatcggtg agcgtgtagc attaaccttt cattattaga agaagggttg
    12481 tagaggggat ctcctattaa aggatgtccg tgatgagata agtgaatgcg aatttgatgg
    12541 gttcttccgg tttccagtcg gcattttatg agactactat tttggatata cttcaaaacg
    12601 tcaatgattg tcatagcatg ttgaccgttt ttagaatcaa tgattcgttt acgtctgtca
    12661 tgacggttcc gtccaatttt gtctctcaaa acttgatgct taggtgaaaa ttttccttca
    12721 actaaagccc aatattcacg ccaaatttct tttcgttcta agcgttgatt gataagggga
    12781 agtataaatg gatttttagc aaataaaaca gctccactgg tctccatatc taggcgatga
    12841 acaacatagc atgtttgtcc agaataggca gatacatgat ttaacagtgc tatttcattt
    12901 ggttggttac cgtgagtttt cataccttca ggtttattaa cgataataag atgttcatcc
    12961 tcataaagac aatcaataag ctctgctctg cccagaggaa tttttttagt agggtaatcc
    13021 tcatcatcaa agattaaggt aatagtatcg ttttcttgga cgacagtttg ccaattaatg
    13081 aattcattgt ttataagtac atgtttttta acacgcaaaa aatgtctaat cttacgtgga
    13141 atcaaaaaat aatcttcaag gagttcttta acggtatgtt caccgtatgg atttctaatt
    13201 tttaatgaaa atgacatggt tttattatag cattttattg aaaattgata taataagtct
    13261 atgaaattat ttgataagtt tatagactta tttcgtgttg atgaagacaa cgacgaaatg
    13321 acaagaaaaa atgaacaaga aactagagaa gaaactagca atttggatgg cgaagaagtc
    13381 tatgatattg atgatattac ccgacctagt aaatcccagt atcagcgagg cattcgtcac
    13441 caaaaggaaa atgctaaatc tcgacttgaa tggctacaaa aagttgatcg ttacctccct
    13501 tctcctaaga atcctatccg tcgtttttgg cggcgctatc gtattgggaa gttgcttttt
    13561 atcgccttaa tggcttttat tcttatattt ggtagctatc ttttttatct ttctaaaact
    13621 gctacagtta gtgatttaca gagtgctcta aaaacaacca ccaccattta tgataagaat
    13681 aaagaatacg ctggaaaact ttcgggacaa aagggaactt atgtagagtt aaatgctatt
    13741 tctgatcatc tcaaaaatgc tgttatagca actgaagata gaacttttta tgaaaataat
    13801 ggtgtaaact ttaagcgatt ttttttggca gtagctacac ttgggaaatt tggcggaggc
    13861 tcaactatta cccagcaatt agcaaaaaat gcttacttgt cacaggacca aacgattaaa
    13921 cgcaaggcgc gtgaattctt tttagcgcta gaattgacta agaaatacag caaggctgag
    13981 attctgacca tgtatcttaa taactcttat tttggtaatg gtgtttgggg tgtggaagat
    14041 gctagtagga aatattttgg aaccagtgct gctaatttga cagtagatga ggctgctaca
    14101 cttgctggaa tgcttaaagg accagaagtt tataatcctt attattctgt tgaaaatgca
    14161 actaaccgaa gagatactgt tttggcagcg atggttgatg caggtcaact aaccaagtct
    14221 caagctaaag aagctgcttc aattggtatg aaaaatagat tagcagatac ctatgctggt
    14281 aaaattaatg attacaagta cccatcctat tttgatgctg ttgtcaatga agccattgat
    14341 acttatggta tttcagaaaa agatattgtc aataatggct ataagattta cactgcatta
    14401 gatcaaaatt atcaatcagg aatgcaaaaa acatttgatg atactagcct cttccctgta
    14461 tctgactatg atggtcaatc tgcccaaggt gctagcgtag ctctagatcc aaaaactggt
    14521 ggtgtaagag gtttagttgg acgtgttcaa agtactaaag atgcacagtt tagaagtttt
    14581 aactacgcga ctcaatcaaa gagaagtcca gcctcaacca taaaaccttt agttgtttat
    14641 tcaccagcta ttgcttcagg atggtcaata gataaagaac ttcctaataa agttcaagat
    14701 tttcatggtt ataaaccaag taactatggt ggaattgaga ctgaaagtat cccgatgtat
    14761 caagcactag ctaattctta taatattcct gctgtttata ctttagataa actaggaatc
    14821 aataaagctt ttacttatgg caggaagttt ggtctaaata tgtcatcagc aaacaaagag
    14881 ctaggagtgg cgctaggtgg aagtgtaact actaatcctt tagaaatggc acaagcttac
    14941 tcaacttttg caaatgatgg tataatgcat cgcgctcatt tgattactcg aattgagaca
    15001 gctaatggaa aattagtaaa gcaatttaca gataagccta agcgagttat tagtcgctca
    15061 gtggctagta aaatgactag tatgatgtta ggaacattct caaacggtac agctattaat
    15121 gccaatgttt atggttatac aatggcaggt aaaactggaa caaccgaaac agattttaac
    15181 cccaatttat ctggagacca gtgggtagtc ggctatacgc ctgatgtcgt tattagtcaa
    15241 tgggttggct ttaagaacac agataaacac cattatttga cagattctag tgcaggtaca
    15301 gcttcaaata tatttagcac gcaagcttca tatatactac cgtatactaa ggggtctagt
    15361 tttactcata ttgagaatgc ttatttccaa aacggtattg gttcagttta taatgcacaa
    15421 gatgctagta atacaacgaa ccaagaatca cgctcaatta ttaatgattt aaaagattcg
    15481 gcaagtaagg ctgcacaaga tatttctaga gctgtggaag attcaaattt ccaagagaag
    15541 gtaaaagatg cttggaatag tctaaaggac tactttagat agcttgcagg attatacaaa
    15601 tcatgttaaa ataacactta tggaggcgtt atggcacaaa agaaagcaag cctagcgtgt
    15661 acagaatgcg gaagccgaaa ttactcaatc ggagtaagta gcacccccaa gccaacacga
    15721 ctagaagtaa ataaattttg taaatattgt aagaaatata caatacacaa agaaacgcgt
    15781 tgatatagcg taacaaactg caaaaaggag aactgataat gtttgtcaaa gggatttttc
    15841 aagtattgcg tgacactact tggccaaatc gaaaacaacg ttggaaagat tttatttcca
    15901 tcttggaata cactgtattt ttcacaatcg ttatctacat ctttgacaaa ctgttagctg
    15961 caggagtcat ggacttgatt aatcgatttt aataatagat tattttagat agaaacaacc
    16021 caaaccctta gattttacta agggt
//
LOCUS       DATRUK010000049        15664 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.049, whole genome shotgun sequence.
ACCESSION   DATRUK010000049 DATRUK010000000
VERSION     DATRUK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15664
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.049"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            26..727
                     /locus_tag="VBE34_001449"
     CDS             26..727
                     /locus_tag="VBE34_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEO7264160.1"
                     /translation="MRVITVKNDIEGGKIAFTLLEEKMKTGAQTLGLATGSSPITFYE
                     EIVKSNLDFSNMVSINLDEYVGIAASNDQSYSYFMHKHLFDAKPFKENNLPNGLAKDL
                     KKEIKRYDAVINANPIDFQILGIGRNGHIGFNEPGTPFDITTHVVDLAPSTIEANSRF
                     FNSIDDVPKQALSMGIGSIMKSKTIVLVAYGIEKAEAIASMIKGPITEDMPASILQKH
                     DDVVIIVDEAAASKL"
     gene            complement(799..1755)
                     /locus_tag="VBE34_001450"
     CDS             complement(799..1755)
                     /locus_tag="VBE34_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO7264161.1"
                     /translation="MGLLVDGKWVDQWYDTASTGGKFVRTVTQFRHWVTKDGSAGPSG
                     DAGFKAESGRYHLYVSLACPWASRVLIMRKLKNLESHISISIVNPLMLENGWTFQEYK
                     GVIPDMINQSQYLYQIYQASQSDYTGRVTVPVLWDKKCHTIVNNESSEIMRMLNTAFN
                     HITGNTDDYYPDSLQGQIDEMNNFIYPKINNGVYKAGFATSQNVYQKEVETLFTALDQ
                     LEKHLSDNHYLVGEQFTEADIRLFTTLVRFDTVYYGHFKCNLKALHDYPHLWHYTKRI
                     YNLPGIAETINFDHIKKHYYGSHKTINPTGIIPAGPNLDWTI"
     gene            1851..2570
                     /locus_tag="VBE34_001451"
     CDS             1851..2570
                     /locus_tag="VBE34_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO7264162.1"
                     /translation="MRLDKLLGQAGFGSRNQVKKLIRSRQVSVDGQIVTKDNVIVDSG
                     LQSIFVGKERVCLKESSYYLLYKPSGVVSAVRDSEHKTVIDLISEKDKVEGLYPIGRL
                     DRDTEGLLIITNNGPLGYRMLHPKHHVAKTYYVEVNGFLERDAITFFEEGVVFDDGTK
                     CKPAELTIDTANNDKSTARITITEGKFHQVKKMFLAYGVKVIYLRRISFGDLRLDMNL
                     KPGQYRRLRDSEAAILKRYLD"
     gene            2643..3794
                     /locus_tag="VBE34_001452"
     CDS             2643..3794
                     /locus_tag="VBE34_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7264163.1"
                     /translation="MNSKLSLKLTILSISIFLMSHLAIAPAIPKLYAFYHTQNPHIGL
                     ASVESLVTIPAMMITIFVILSNFVVTKLGKKNTVLLGLCLILMSGFISFFTSNFSLAM
                     ASRLLLGIGIGLYNSLSISIITDLYEADERASMIGLRTASLNIGKALTTFIVGLVLAI
                     GVNYIYLVYLLVIPVFFFFWKNVPEVENQTHTLKASTTFDTKAALLMLITFLVGIAYI
                     GATVKIPTLLVTKYHYATSFSSNMLTLLAFSGILVGSVFGKLVKAFQEKTLLIMILAM
                     GIGNVLFALANNQIIFIVASILIGASFVGTMSSVFFYISKNYAKEHNNFITSLALTAG
                     NIGVILTPLILTKLPSQLHLEPFITPFLITSGLMVINVFVYLVLMSKNK"
     gene            3846..4793
                     /locus_tag="VBE34_001453"
     CDS             3846..4793
                     /locus_tag="VBE34_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEO7264164.1"
                     /translation="MLIAKDKQGNLINLLESHPGKGQYFCPTCCSAVRLKAGRIMRRH
                     FAHISLKNCQFHHENESNEHLQLKAKLYMSLSRENETMLEHHLPEINQIADLFVNETL
                     ALEVQCSRLSEQRLRERTKAYLQADFQVRWLLGEKLWLKHRLTNLHKQFLQFSQSIGF
                     HIWELDLRLEVLRLKYLIYEDLRGHVYYLSKTCPLSGDVLAFLKWPYQSKNLNFYKVK
                     QDRNIRDYVRQQLRYGNQFWLRKQEKAYLSGQNLLTQELMMFFPQIQPPRVDTDFCQI
                     TNSLTSFYQNFTNYYQKNKNNLDQTLYPPVFYDKIVTKN"
     gene            4809..6614
                     /gene="pepF"
                     /locus_tag="VBE34_001454"
     CDS             4809..6614
                     /gene="pepF"
                     /locus_tag="VBE34_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO7264165.1"
                     /translation="MSDNRSHIEEKYQWDLTTVFATDELWETEVVELTQAIDDAKGFS
                     GHLLDSSQSLLEITEVELELSRRLEKVYVYASMKNDQDTTVAKYQEFQAKATALYAKF
                     SETFSFYEPELLQLSESDYQSFLLEMPDLQKYDHFFEKIFANKPHVLSQNEEELLAGA
                     SEIFGAAGETFEILDNADMVFPVVKNAKGEEVELTHGNFISLMESSDRTVRKEAYQAM
                     YSTYEQFQHTYAKTLQTNVKSQNFKARVHHYQSARQSALSANFIPEEVYETLIKTVNH
                     HLPLLHRYMKLRQKVLGLDDLKMYDVYTPLSQMDMSFTYDEALKKSEKVLAIFGEAYS
                     ERVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRENQPYVYGDYSIFLAEIASTTNENILTETLLKEVKDDKNRFAILNHYLDGFK
                     GTIFRQTQFAEFEHAIHVADQEGQVLTSEYLNNLYAELNEKYYGLTKEDNHFIQYEWA
                     RIPHFYYNYYVFQYATGFAAANYLAERIVNGNPEDKEAYLNYLKAGNSDYPLNVIAKA
                     GVDMTSADYLDAAFRVFEERLVELENLVTKGVHND"
     gene            6809..7435
                     /locus_tag="VBE34_001455"
     CDS             6809..7435
                     /locus_tag="VBE34_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEO7264166.1"
                     /translation="MKKLTFIWDLDGTLIDSYVPIMEALEETYRHFGLIFDKELIHEY
                     ILQESVGQLLVNLSEEEQIPHEKLKAYFTKEQESRDSKIHLMPYAKEILEWTKEQDIP
                     NFMYTHKGASTHSVLETLQISHYFDEILTGVSGFERKPHPQGINYLVKRYSLDKSMTY
                     YIGDRPLDLEVAQNAGIKSINLRLENSKENYNISSLKDIISLDFTRLD"
     gene            7509..8216
                     /locus_tag="VBE34_001456"
     CDS             7509..8216
                     /locus_tag="VBE34_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEO7264167.1"
                     /translation="MVQSYSKNANHNMRRPVVKEEIVQYMRQHQKQNNGCLAELEAFA
                     KQENIPIIPHETATYFRFLMQTLQPKHILEIGTAIGFSALLMAENAPEAKITTIDRNE
                     EMIALAKENFAKYDNHNQITLLEGDAVDVLQTLDKSYDFVFMDSAKSKYIVFLPQVLK
                     HLDVGGVVVLDDIFQGGDIAKPIDEVRRGQRTIYRGLQRLFDSTLQHPDLTATLVPLG
                     DGLLMIRKNADHIVLED"
     gene            8277..9206
                     /gene="prsA"
                     /locus_tag="VBE34_001457"
     CDS             8277..9206
                     /gene="prsA"
                     /locus_tag="VBE34_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEO7264168.1"
                     /translation="MKTRSKLAAGFLTLMSVATLAACSGKTSNGTNVVTMKGDTITVS
                     DFYDQVKTSKAAQQSMLTLILSRVFDTQYGDKVSDKKVSEAYNKTAKGYGNSFSSALS
                     QAGLTPEGYKQQIRTTMLVEYAVKEAAKKELTEANYKEAYKNYTPETSVQVIKLDAED
                     KAKSVLKDVKADGADFAKIAKEKTTATDKKVEYKFDSAGTSLPKEVMSAAFKLDKNGV
                     SDVVSTVDSTTYKTSYYIIKVTDKTEKKSDWKSYKNRLKEVILKDKTSDRSFQNKVIS
                     KALEKANVKIKDKAFAGILSQYATTSGSSSLKK"
     gene            9448..9933
                     /locus_tag="VBE34_001458"
     CDS             9448..9933
                     /locus_tag="VBE34_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEO7264169.1"
                     /translation="MATFQIKEKMFSLGGKFTITDLTGLPCYHVEGSLFPLPKTFKVF
                     DEEEHLISQIEKKVLSFLPKFNVTLANGNHFTIKKDFSFLKPHYTIEDLDMEVKGNFW
                     DMDFQLLKDNQVIANISQQWFRMTSTYQVEVYSETYNDLTISLVIAIDYVKELEKNAS
                     N"
     gene            9949..12567
                     /gene="alaS"
                     /locus_tag="VBE34_001459"
     CDS             9949..12567
                     /gene="alaS"
                     /locus_tag="VBE34_001459"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEO7264170.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHSVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPEWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDATAKVQALS
                     DSLRDLQKENVELKEKAAAAAAGDVFKDIQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKGLAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAENL"
     gene            12649..13365
                     /locus_tag="VBE34_001460"
     CDS             12649..13365
                     /locus_tag="VBE34_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEO7264171.1"
                     /translation="MVGLGTVINVILIIVGGFVGLFLKNFLKESLQKSLMQAMGVAVL
                     FISISGVLEKMMLVEKSHLISNHTNMMIITLALGTVLGELLSLDSYIDRFGDYLKQKT
                     GSGNDIKFVEAFVTSTCTVCIGAMAVVGSIQDGIAADHSILFAKGMLDMIIIAIMTVS
                     LGKGALFSALPVALLQGSLTIVAFFMGSLLNPSSLDYLNLVGNMLIFCVGVNLLFNLN
                     IKVINMLPAIILAILWGSFI"
     gene            13453..14271
                     /locus_tag="VBE34_001461"
     CDS             13453..14271
                     /locus_tag="VBE34_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEO7264172.1"
                     /translation="MNLLFSIDDMYVDHFKVMLYSLVRQTKNRKLEIYVLQKTLLKRH
                     TELIQYTQNLEVGYHPIIVGTEVFAQAPTTDRYPDTIYYRLLAHKFLPETLDRILYLD
                     ADMLCLNDFSSLYDMELGDQLYAAASHNTDGKFLDYVNKLRLKNVELESSYFNTGVLL
                     MNLPAIRKVVHQQTILDYIMQNRGRLILPDQDILNGLYANLVKPIPDEIYNYDARYSL
                     IYQLKSRNEWDLEWVINHTVFLHFAGRDKPWKKDYRGRYSGLYKFMAKEALTKN"
     gene            14472..14660
                     /locus_tag="VBE34_001462"
     CDS             14472..14660
                     /locus_tag="VBE34_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264173.1"
                     /translation="MKKEFIITGRMVARVMETLVCLCMVSNLVLDMLDIIEINEIVFC
                     VLVASIAGLEFCLQKERE"
     gene            14692..14985
                     /locus_tag="VBE34_001463"
     CDS             14692..14985
                     /locus_tag="VBE34_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264174.1"
                     /translation="MVMTYIGVPKEQKALSRRGKQLEKINQGGKTLMSQMIQDLVGST
                     CMIYLEDGFQTHPYEILKVDEDWVQIRRNLKRSKEEVRILRIHDIKELRPVNK"
     gene            15030..15245
                     /locus_tag="VBE34_001464"
     CDS             15030..15245
                     /locus_tag="VBE34_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7264175.1"
                     /translation="MGLILKNRLKELRARDGINQTELAKLTGVSRQTISLIERGEYTP
                     SVIIAMKISQVFKESLENVFSLVSTEE"
     gene            15248..>15664
                     /locus_tag="VBE34_001465"
     CDS             15248..>15664
                     /locus_tag="VBE34_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEO7264176.1"
                     /translation="MKNFIKQSDTTFVRIIKSLLIGGFIGAILGSVGALFNIFGQDKY
                     LSEINIVQYFLWVSRIVVIITALFSLIYLYQIQKYQKVFFNVDESQSEEIYRQINLRH
                     SYGMTFVSISIVLSIFNTLFNYKLNIFDDSVTLVIPI"
BASE COUNT         5249 a         2457 c         3031 g         4927 t
ORIGIN      
        1 tataccaaat aaaaggagag taaacatgag agttattaca gttaagaacg acattgaggg
       61 gggaaaaatt gcatttactc ttttagaaga gaaaatgaaa actggtgctc aaactttagg
      121 attggcaaca ggaagtagcc caatcacctt ttacgaagag attgtaaaaa gtaaccttga
      181 tttttcaaat atggtttcta ttaaccttga tgaatatgtt ggaattgccg caagtaatga
      241 tcaaagttac tcttatttta tgcataaaca tctttttgat gctaaacctt ttaaggaaaa
      301 taaccttcca aacggcttgg ctaaagattt gaaaaaagaa ataaaacgat acgatgctgt
      361 cataaatgct aatccaattg attttcaaat tcttggaatt ggtcgtaacg gtcatattgg
      421 ttttaatgaa ccaggtacac cttttgatat tacaacacat gtggttgact tagctccaag
      481 tacaattgaa gctaatagtc gtttctttaa tagtattgat gatgtaccaa agcaagcctt
      541 atcaatgggt ataggttcta ttatgaaatc aaaaacaatc gtattagtgg cgtatggtat
      601 tgaaaaagca gaagcgattg caagtatgat taaaggacct atcacagaag atatgccagc
      661 aagtatcctc caaaaacatg atgatgttgt gattattgtg gatgaagcag cggcatcaaa
      721 gttgtaaagt aaataataaa aaagtaagga tgaatctcaa tgagaaaaaa ttcattctta
      781 cttttttatt atgctttatt aaattgtcca gtctaagtta ggtccagctg gaataatacc
      841 ggtagggtta atggttttat ggctaccgta atagtgtttt ttgatatgat caaaattaat
      901 tgtctcagca attccaggta ggttgtaaat acgttttgta taatgccata aatgtgggta
      961 atcatgtagc gcttttaaat tacatttaaa atgaccataa taaacggtgt caaagcgaac
     1021 taaagttgtg aaaaggcgaa tatcagcttc ggtaaattgt tctccaacaa gataatggtt
     1081 atcagataag tgtttttcta actgatctaa agctgtgaat aaggtttcaa cttccttttg
     1141 ataaacgttc tgagaagtag caaaaccagc tttatagaca ccattattta tttttggata
     1201 aataaaatta ttcatttcgt caatttgtcc ttgtaaggaa tcgggatagt aatcgtcagt
     1261 atttccagta atatgattaa atgcagtatt taacatgcgc ataatttcag atgattcatt
     1321 gttaacgatt gtatggcatt ttttatccca aagaacaggg actgtcacgc gtcctgtata
     1381 atcactttga cttgcttgat agatttggta aaggtattgg ctttggttaa tcatatcagg
     1441 aataacgcct ttatattctt gaaaagtcca accattctcc aacattaaag ggttaacaat
     1501 ggagatagag atatgagatt ctagattttt aagctttctc atgattaata cacgtgatgc
     1561 ccaagggcag gctagtgaaa cgtagagatg atatcgtcca gattctgctt taaaacctgc
     1621 atccccacta ggccctgctg aaccatcttt agtaacccaa tgacggaatt gtgtaacggt
     1681 tcggacgaat ttaccaccag ttgatgcagt atcataccat tgatcaaccc atttgccatc
     1741 tactaaaagt cccatgataa tctcctttgt ttttctatta ctaattagta gtataaagat
     1801 tttaaaagga attagcaaat gtttaactta cagtatggta aactaagggt atgcgtttag
     1861 ataaattatt aggtcaggca ggatttgggt ctagaaatca ggttaagaaa ttaattcgtt
     1921 ctaggcaagt ttctgtagat ggtcaaattg ttacaaagga taatgttatt gtagatagtg
     1981 gtctacaatc gatttttgta ggaaaagaga gagtctgcct aaaagaaagt tcttattacc
     2041 ttctttataa gccatcagga gttgtttcag cagttagaga ttcagaacat aaaacagtca
     2101 tagacttaat tagcgaaaaa gataaggtgg agggattgta tccgattggt cgtctagatc
     2161 gtgatacgga agggctttta ataatcacga ataatggtcc actgggttac cgaatgttac
     2221 atccaaaaca ccacgttgca aaaacgtatt atgttgaagt aaatggtttc ttggaaagag
     2281 atgctattac cttttttgaa gaaggagttg tatttgacga tggaacaaag tgtaaaccag
     2341 ctgaattaac gattgatacg gctaataatg acaaaagtac cgcaagaata actatcacag
     2401 aagggaaatt tcaccaagta aaaaagatgt ttcttgcata tggcgttaaa gttatatact
     2461 tgaggcgtat ttcttttggt gacttgaggc ttgatatgaa tttgaaacca ggtcagtatc
     2521 gtagattgag agattcagag gcagctatct taaaaaggta tttagattag tgaaacgact
     2581 cacttgacct tgcttagtat tcttcatata cttataagtg attattttaa aggagaaaac
     2641 tattgaattc aaaactttca ttgaaattga ctattttatc gatttctatc tttttgatgt
     2701 ctcacttagc aattgctcct gcaattccta aattgtatgc cttttaccat actcaaaacc
     2761 ctcatattgg actagcttca gtagagagtt tagttactat tccagctatg atgattacta
     2821 tttttgttat tttgagtaat tttgtagtca caaaacttgg caaaaaaaac acggtcttac
     2881 ttggtttatg tttaatctta atgtctgggt ttatctcatt ttttacaagt aatttttcac
     2941 ttgcgatggc tagtcgacta cttttgggta ttggtattgg tctctataat tcgctatcaa
     3001 ttagtatcat aacggattta tatgaggctg atgagagagc cagtatgatt ggtcttagaa
     3061 cagcatcact taatatcggt aaagccttaa ctacatttat tgttggattg gttttagcta
     3121 ttggtgtgaa ttatatttat ttggtttacc ttctggttat tcctgttttc tttttctttt
     3181 ggaaaaatgt tccagaggtt gaaaatcaaa cacataccct taaagctagt acaacatttg
     3241 atactaaagc ggctttgttg atgctcatca cgttcttagt aggtattgct tatataggag
     3301 caactgtcaa aattccaacc ttactagtga caaaatatca ctacgcgacg agcttctcta
     3361 gtaatatgtt aactctcctt gcttttagtg gtattttagt tggatctgta tttggaaaat
     3421 tagtcaaagc attccaagag aagacgctat taatcatgat tctagctatg ggaataggta
     3481 atgtgctatt tgctttagct aataatcaga tcatttttat cgtagctagt attttaatag
     3541 gagctagttt tgttggcacc atgtcttctg tattctttta tatttcaaaa aactatgcaa
     3601 aagaacataa taactttatc acaagccttg ccttaacagc tggtaatata ggagttattt
     3661 tgacaccgct tattttaacg aaacttccaa gccagctaca tctggaacct tttataacac
     3721 catttttaat aacaagtggg ctgatggtga ttaatgtctt tgtttaccta gttttaatgt
     3781 caaaaaataa ataaaaagtt atccgaaagg atagcttttt tcgaatagta aaagtaggag
     3841 aaaaaatgtt aattgctaaa gacaaacaag gaaatttaat taatctactc gagtctcatc
     3901 cgggaaaggg acagtatttt tgtccaactt gttgttcagc tgtaaggtta aaagcaggtc
     3961 gaatcatgag aagacacttt gcacatatta gtctaaaaaa ttgtcagttt catcatgaaa
     4021 atgagtctaa tgagcacttg caattaaaag caaagttata tatgagttta agtagagaga
     4081 atgaaactat gctcgagcat catttgccag aaattaatca gattgcagat ttatttgtga
     4141 atgagacatt ggctttagaa gttcagtgta gccgactatc agagcagcga ctcagggagc
     4201 gaacaaaagc atatctacag gcagactttc aagtgagatg gttactaggg gaaaaactct
     4261 ggttaaagca tcgtttaaca aatctacata aacaattttt acaattttct caaagtatcg
     4321 gatttcatat ttgggagtta gatttgaggt tagaagtact gagactaaaa tatttgattt
     4381 acgaagattt acgcggacat gtctattact taagtaaaac ttgccctctt agtggagacg
     4441 ttctagcatt tttgaagtgg ccataccaat caaaaaatct aaatttttat aaggttaaac
     4501 aagacagaaa tatcagagat tatgtgcgtc agcagttacg ctatggcaat caattttggt
     4561 tacgaaaaca agaaaaggcc tacctttctg gtcagaatct attaacccaa gagctaatga
     4621 tgttttttcc tcaaatacag ccaccacgtg tagatactga tttttgtcaa ataacaaatt
     4681 ctctgacatc gttttatcag aattttacaa attattatca aaaaaataag aataacttgg
     4741 accaaacact ttatccccct gtattttatg ataaaatagt cactaagaat taaactggag
     4801 gatacaaaat gtcagacaat cgctcacata ttgaagaaaa ataccaatgg gatttaacta
     4861 ctgtctttgc gactgatgag ttatgggaaa ctgaagtggt agagcttact caagccatcg
     4921 atgacgcaaa gggtttttca ggtcatctgt tggactcaag ccaatcttta ttggagatta
     4981 cggaggttga actagagtta tctcgccgtt tagaaaaagt ttatgtctat gcttcaatga
     5041 aaaatgacca agacaccact gttgcgaaat atcaagaatt tcaagcgaaa gcgacagcgc
     5101 tctatgcaaa atttagtgaa acattttcgt tttacgagcc agaactattg caactttcag
     5161 agtctgatta ccagtcattc cttttagaaa tgcctgactt gcaaaaatat gatcatttct
     5221 ttgagaaaat atttgcaaat aaacctcatg tcttatctca aaacgaagaa gaattgttag
     5281 ctggtgctag tgagattttt ggagcagctg gtgagacatt tgaaatcctt gataatgcag
     5341 atatggtctt cccagttgta aaaaatgcaa aaggtgagga agttgagctt acacatggta
     5401 atttcatttc ccttatggaa tctagcgata ggacggttcg aaaagaagca tatcaagcta
     5461 tgtatagcac atatgaacaa ttccagcata cttacgctaa aacattacaa acaaatgtga
     5521 aatctcaaaa ttttaaggct cgtgtgcatc attatcaatc agcacgccaa tcagctctat
     5581 ctgccaattt tattccagaa gaggtctacg aaactctaat taagacagtt aatcatcatt
     5641 taccgctctt acatcggtat atgaagttgc gtcaaaaagt tcttgggcta gatgatttga
     5701 agatgtatga cgtatataca cccctttctc aaatggatat gagttttact tatgatgaag
     5761 ctctaaaaaa atcagagaag gttctggcta tttttggcga agcatattct gaacgtgttc
     5821 atcgtgcttt tactgaacgt tggattgatg tccatgttaa caaaggaaag aggtcaggcg
     5881 cttattcagg tggatcttat gacactaatg ctttcatgct tcttaactgg caagatactt
     5941 tagacaatct ttatacgctg gtgcatgaaa ctggacatag tctacactcg acgtttactc
     6001 gtgaaaatca accttatgtt tatggtgatt attctatttt cctagctgaa attgcatcaa
     6061 caacaaatga aaatatatta acagaaaccc ttttgaaaga agttaaagat gataaaaatc
     6121 gctttgcaat tctcaatcat tatttggacg gtttcaaagg aacaattttt agacaaacac
     6181 agttcgctga attcgaacat gccattcatg tagctgatca ggaggggcaa gtactaacta
     6241 gcgaatactt aaacaacctt tacgctgagc ttaatgaaaa atattatggt ttgactaaag
     6301 aagataatca ttttattcaa tatgaatggg caagaattcc acacttttac tacaattatt
     6361 atgttttcca atatgcaacc ggcttcgcag cagcaaatta tttagcagag cgtattgtaa
     6421 acggtaatcc ggaggataaa gaggcctacc taaattacct taaagctggg aattcagatt
     6481 accctctaaa cgtcattgct aaagccggtg ttgatatgac tagtgcagac tatctggatg
     6541 cagcttttag agtatttgaa gagcgtttag tggaactgga aaatcttgta acaaaaggcg
     6601 tccataatga ttaagttgaa aatatagcta ttgaagtaaa accagtcaac tagactggtt
     6661 tttgatagtg atcagttatt ttatagtttg ttttgagagt ggttttggta aagttagtta
     6721 gtggaaaact caataggtgg aaatacctac ccaagctatg atttttatga gaccaaaact
     6781 gctattgtca aaaaagaagg gaaataggat gaaaaagctt acttttattt gggatttaga
     6841 tgggacatta atagattcgt atgtaccaat tatggaagct cttgaagaaa cctatcgtca
     6901 ttttggctta atatttgata aagaattaat ccatgaatat attttacagg aatcagtggg
     6961 gcaattattg gtaaaccttt cagaggaaga gcaaatacct catgaaaaac tgaaagcata
     7021 ttttacaaaa gaacaagaaa gtcgagattc taaaatacat ttaatgccat atgcaaaaga
     7081 gattttagaa tggaccaaag aacaagatat tcccaatttt atgtatacac ataaaggagc
     7141 aagtacgcat tcagtgttgg aaaccttgca gatctctcat tattttgatg aaattttaac
     7201 tggtgtttcg ggattcgagc gaaaaccaca tccacaaggg attaattatt tagttaaacg
     7261 atattcttta gataaatcaa tgacttatta cataggagat cgtccactag atttggaggt
     7321 tgctcaaaat gctggtataa aatccataaa cttaaggtta gagaattcca aagaaaacta
     7381 taatatttca agtctcaaag atataatatc acttgatttc actcgtttgg attaaatcat
     7441 tctcagagtt agacattttt tgctataata aaaaagttat tatttaaaat agaaaagagg
     7501 agccaaaagt ggttcaatct tacagtaaaa atgctaatca taatatgcgc cgtccagtag
     7561 ttaaagaaga aattgttcaa tatatgcgtc agcaccaaaa acaaaataat ggctgtttag
     7621 cagagttaga agcatttgct aaacaggaaa atatccccat tatacctcat gaaacagcga
     7681 cttattttcg ttttttgatg caaacattac aacctaagca cattttagaa atcggaacag
     7741 ctattggttt ttcggccctt ttgatggcag aaaatgctcc tgaagcaaag ataacaacaa
     7801 tcgatcgtaa tgaagagatg attgccttgg ctaaggaaaa ctttgcaaaa tatgataatc
     7861 acaatcagat aacccttttg gaaggcgatg ctgtggatgt gttacaaaca ttggataaat
     7921 cttacgattt tgtatttatg gattctgcta agtctaaata tatcgttttt ttacctcaag
     7981 tattaaagca tttagatgtt ggaggagtag ttgttttaga tgatattttc caaggtggag
     8041 atattgcaaa acctatcgat gaagtgagaa ggggtcagcg aactatctac cgaggtctgc
     8101 aacgtttatt tgactcgact ttgcaacatc ctgatttaac tgctactttg gtacctttgg
     8161 gagatggttt attgatgatt cgtaaaaatg cagatcatat tgtactagaa gattaaggca
     8221 tttttaagaa attatgatat aataatggag ttaatgacaa aaaggagtta taaatcatga
     8281 aaacaagatc aaaacttgca gcaggctttt tgacacttat gtcagttgcc acattagctg
     8341 cctgctcagg taaaacatca aatggaacaa atgttgttac aatgaagggc gacactatta
     8401 cagtctctga tttttatgat caagtaaaaa catcaaaagc tgcacaacaa tcaatgctta
     8461 cattgatcct ctcacgtgtt tttgatacac agtatggtga taaagtttca gataaaaaag
     8521 tatcagaagc ttataataag acagctaaag gctatggtaa ttcattttca agcgcacttt
     8581 cacaagcagg tttgactccg gaaggttaca aacaacaaat tcgcacaact atgcttgtgg
     8641 aatatgctgt aaaagaagca gctaagaaag aattaacaga agcaaactat aaagaagcat
     8701 ataaaaacta tactcctgaa acttctgtac aagtaatcaa attggatgca gaagataaag
     8761 ctaaatctgt ccttaaagat gtaaaggctg atggagctga ttttgcaaag attgcaaaag
     8821 aaaaaacaac agctactgat aaaaaagttg agtataaatt tgattctgca gggacaagcc
     8881 tccctaaaga agttatgtca gcagccttta agctagataa aaatggtgtt tcagatgtgg
     8941 tttcaacggt tgattcaaca acttataaaa caagttacta catcattaaa gtaactgata
     9001 agacagagaa aaaatctgat tggaaatctt acaaaaatcg cttaaaagaa gttattctta
     9061 aggataaaac aagcgataga tccttccaaa ataaagtgat ttcaaaagcc ttagaaaaag
     9121 ctaacgttaa gattaaagat aaagcatttg caggcatctt atcacaatat gctacaacaa
     9181 gtggttcatc atcacttaaa aaatagttga ccaagtctta aaaagactgt ataattaata
     9241 ctattgagaa tgaaatttca acgattagat acagaaaagt gacggttgct gcgagtcatg
     9301 gataatcaga aaaagatgct actcaatggg gtaaaactaa aaataaatga gaatgacaag
     9361 ttcattgaat gaaggtggta ccgcggtttt cgcccttcgt aatggacttg tcttttgcta
     9421 tttgtcaact ttcgaggagg aagagatatg gctacttttc aaattaaaga aaaaatgttt
     9481 tctctaggcg gtaaatttac tattacagac ctgactgggc ttccctgtta tcacgttgaa
     9541 ggaagtttgt tccctctacc taagactttt aaagtctttg acgaagagga acatctcatt
     9601 agtcagattg agaagaaggt tttaagtttt ttaccaaagt ttaatgtgac attagccaat
     9661 gggaatcatt tcacgattaa gaaagatttc tcatttttga aacctcatta caccattgaa
     9721 gaccttgata tggaagttaa gggaaatttt tgggatatgg atttccaact cttaaaagac
     9781 aatcaagtta tagcaaacat atctcaacag tggtttcgta tgacttcaac ttatcaggta
     9841 gaagtttaca gtgaaactta caacgatttg acaatttcgc ttgtcatagc gatcgactat
     9901 gtcaaagagt tagaaaaaaa tgcatcaaac taattttagg agagaataat gaaagaatta
     9961 tcgtctgcac aaatccgcca aatgtggttg gatttctgga aatccaaagg acattccgtt
    10021 gagccttcag ctaacttggt tcctgttaat gacccaacgc ttctttggat caactcaggt
    10081 gttgcaacct tgaaaaaata ttttgatggt tcagtgattc cagaaaatcc acgtattacc
    10141 aatgcacaaa aatcaattcg tactaatgat attgaaaatg ttggtaaaac agcacgtcac
    10201 catactatgt ttgaaatgct tggtaacttc tcaattggag actatttccg tgatgaagct
    10261 attgagtggg gatttgaact cttgacaagt cctgaatggt ttgacttccc taaagacaag
    10321 ctctacatga cttattaccc agatgacaag gattcatata accgttggat tgcttgtggc
    10381 gttgaaccaa gtcacttggt gccaatcgag gataacttct gggaaatcgg tgctggtcct
    10441 tcaggtccag atactgagat tttcttcgac cgtggtgaag attttgatcc agaaaatatt
    10501 ggacttcgtc ttttggctga agatatcgaa aacgatcgtt acatcgaaat ctggaacatt
    10561 gttctctcac aattcaatgc tgacccagcc gtaccacgtt cagaatacaa agaattgcca
    10621 aacaaaaaca ttgatacggg tgcgggtctt gaacgtcttg cagctgttat gcaaggggca
    10681 aaaacaaact ttgaaactga ccttttcatg cctatcatcc gtgaagtaga gaaattgtca
    10741 ggtaaaactt acgatccaga tggcgacaac atgagcttca aggttatcgc tgatcacatc
    10801 cgtgcgcttt catttgccat cggtgatggt gcgcttcctg gaaatgaagg tcgtggttat
    10861 gttcttcgtc gtcttctccg tcgtgcggtt atgcacggtc gccgtcttgg catcaacgaa
    10921 actttccttt acaaattggt tccgactgtt ggacaaatca tggaaagcta ctatccagaa
    10981 gtgcttgaaa aacgtgattt tattgagaaa atcgttaaac gtgaggaaga aacatttgct
    11041 cgtactatcg atgctggtag cggtcactta gattcattgc ttgcgcagct taaggctgaa
    11101 ggtaaagaca cacttgaagg taaagatatc ttcaaacttt atgatactta tggattccca
    11161 gttgaattga cagaggaatt ggcagaagat gcaggctata agattgacca cgaaggcttt
    11221 aagtcagcca tgaaagaaca acaagaccgt gcgcgtgcag ctgttgttaa gggtggttcg
    11281 atggggatgc aaaatgaaac cctagctggt attgttgaag aatcacgatt cgaatacgac
    11341 acatatagtc ttgaatcaag tctttcagtt atcattgctg ataatgaacg tactgaggct
    11401 gtttcagaag gtcaagccct tcttgtcttt gctcaaacac cattctatgc tgaaatgggt
    11461 ggacaggttg ctgacacagg tagaatcaaa aatgataagg gtgacacagt tgctgaggtt
    11521 gttgatgttc aaaaagcacc aaatggtcaa cctctacaca ctgtaaacgt tttggcatca
    11581 ctttcagttg gaacaaacta cacacttgaa atcaacaaag agcgtcgttt ggctgttgag
    11641 aaaaaccaca cagcaactca cttgctccat gcagcccttc acaatgttat cggtgaacac
    11701 gcaactcagg ctggttcatt gaacgaagaa gaattcttgc gctttgattt tactcacttt
    11761 gaagcagtaa gcaatgagga acttcgtcac attgaacaag aagttaatga gcaaatttgg
    11821 aacgctctta caatcacaac gactgaaact gacgttgaaa ccgcaaaaga gatgggagca
    11881 atggcacttt ttggtgagaa atatggtaaa gtggttcgtg tggttcaaat tggtaattat
    11941 tctgttgaac tttgcggtgg aactcactta aataattctt cagaaatcgg tctcttcaag
    12001 attgtcaaag aagaaggtat tggttcaggc actcgtcgta ttattgcagt tactggtaga
    12061 caagcttttg aagcttatcg taaccaagag gatgcactaa aagagatcgc tgctactgta
    12121 aaagctccgc aattgaaaga tgcaacagct aaagtacaag ctcttagcga ttcacttcgt
    12181 gatcttcaaa aagaaaatgt agagcttaaa gaaaaagcag cagcagcagc agctggtgat
    12241 gtctttaaag atattcaaga agctaagggc gtgcgcttca ttgctagtca agttgatgtt
    12301 gcagacgcag gtgcacttcg tacatttgct gataactgga aacaaaaaga ctactctgat
    12361 gtgcttgttc tcgtagcagc tattggtgag aaggttaatg tccttgttgc aagcaaaact
    12421 aaagatgtcc acgctggtaa catgatcaaa ggattggcac caattgtagc aggtcgtggt
    12481 ggtggtaaac cagacatggc tatggcaggt ggtagcgatg caagtaaaat tgcagagctg
    12541 ctagcagcag ttgcagaaaa tttataagaa gtacctctca tttagttagt aaataaaaca
    12601 ggcaaagggc agaacatcgt tctgttcctt tgtgtatata ggagaaatat ggttggttta
    12661 ggaacagtta tcaatgttat tcttattata gttggaggct ttgtaggttt atttttgaaa
    12721 aattttttga aagaatcact tcaaaagagc ctcatgcaag ccatgggagt cgcagtttta
    12781 tttatttcaa tatcaggtgt tttggagaaa atgatgttag tcgaaaagtc acatctcatc
    12841 tctaatcata caaatatgat gattattact cttgctctag gtacggtact tggagagttg
    12901 ctatcactag attcgtatat tgatagattt ggggactatc ttaagcaaaa aacaggaagt
    12961 ggtaacgata ttaaatttgt ggaagcattc gtcacctcaa cttgtactgt ttgtattgga
    13021 gcaatggccg ttgttggtag cattcaagat ggtatagcag cagatcattc gattttattt
    13081 gctaagggaa tgttagacat gattattatt gcaattatga cagtgtcttt agggaaaggg
    13141 gctctatttt ctgcacttcc tgtcgcttta ctacaaggta gccttacgat tgttgctttt
    13201 ttcatgggga gcctacttaa tccatcatct ttagattacc ttaacttagt aggaaatatg
    13261 cttatattct gtgtcggtgt taatttactc tttaacctaa atattaaagt tatcaatatg
    13321 ttgccagcaa ttattttagc tatattatgg ggaagtttta tataaaagat aaccaagttt
    13381 taactcaaac ttggtttttc tttagacata tgttaggatg aaagaagata tactacggag
    13441 aggatccaac tcatgaatct attattttct attgatgata tgtacgtaga tcattttaaa
    13501 gtgatgcttt attcgcttgt ccgacaaacc aagaatcgta agttagagat atatgtctta
    13561 caaaaaacat tactcaaacg tcatacagaa cttattcaat atactcaaaa tttagaagtt
    13621 ggctatcatc caataattgt aggtaccgaa gtatttgctc aggcacctac aactgatcgc
    13681 tatccagata caatatatta tcgacttttg gctcataaat ttttacctga gaccttagat
    13741 aggatacttt atttagatgc agatatgctt tgcttaaatg atttctcaag tttatatgac
    13801 atggagttag gtgatcaact atatgcagcg gcaagtcata atactgatgg taagtttcta
    13861 gattatgtca ataaattacg gttaaaaaat gtagagctag aatcctctta tttcaataca
    13921 ggggtactct tgatgaactt gcctgctata cgaaaagttg ttcatcaaca aactatctta
    13981 gattacataa tgcaaaaccg tggtcgtcta atcttaccag atcaggatat cttaaatggc
    14041 ttatacgcta atttggttaa accaatacct gatgaaattt ataattatga tgctaggtat
    14101 agtcttattt atcaattaaa aagtcgcaat gaatgggatt tagagtgggt tattaaccat
    14161 actgtttttt tacattttgc aggacgagat aaaccatgga aaaaagatta tagaggacgt
    14221 tactcgggcc tttataagtt tatggctaaa gaggctttaa ctaaaaattg aagatttaaa
    14281 aggttactaa attatttcta tttagtaact ttttttgggg tgatatttta atgatatcaa
    14341 taatctttgc taataattat ttataaaaaa gaaaaataat atcaaaggag gagcgtgtta
    14401 aaatcactta atcattgaac aatcaaatta atcattgttt caaaagcata atggttttag
    14461 gagttactca aatgaaaaag gaatttatta ttacaggtcg gatggttgca agagttatgg
    14521 aaaccctagt ttgcttatgt atggtaagta atcttgttct tgatatgctt gatataatag
    14581 aaataaatga aattgtattt tgtgtgttgg tagcatcaat tgcagggctt gagttttgtt
    14641 tgcaaaaaga aagggagtag atagatggaa tttttggctt aatggctttt tatggtcatg
    14701 acgtatattg gtgtacctaa agagcaaaag gcactcagtc gtaggggtaa acaattagaa
    14761 aaaataaatc agggaggaaa aacacttatg tcacaaatga ttcaagattt agtaggaagc
    14821 acatgtatga tttatttaga ggatggtttt caaacgcatc cttatgagat tttaaaggta
    14881 gatgaggatt gggtacagat tcgtcgcaac ttgaaacgaa gtaaagagga ggtacgtatt
    14941 ctccgtattc atgatatcaa ggaattgcgt ccagtcaata aatagagtga ggaataaatc
    15001 tatatatcat tttataaagg agaaaaatga tgggattaat tttaaaaaac aggctcaaag
    15061 aattgcgtgc acgagatggt atcaatcaaa cagaactcgc caaattaacg ggagtctcaa
    15121 gacaaacaat tagcctaatt gaacgcggag aatatacgcc atctgttatt attgctatga
    15181 agatttctca agtatttaaa gaatccctag agaatgtttt tagtttagtg tcaacggagg
    15241 aataacgatg aaaaatttta taaaacaaag tgatactaca tttgttcgaa ttatcaaaag
    15301 tcttttaatt ggtggtttta taggggccat acttggttcc gtaggagctc tttttaacat
    15361 atttggtcaa gataagtact tatctgaaat taatattgtt caatatttcc tatgggttag
    15421 tcgtattgtt gtaattataa cagctctctt tagtctcatc tacctctatc agattcaaaa
    15481 atatcagaaa gtttttttca atgttgatga aagtcaatct gaagaaattt atcgccagat
    15541 taatttaaga cattcttatg ggatgacttt tgtgtcaatt tcaatagtac tcagtatatt
    15601 taatacttta tttaactata aactaaatat ttttgatgat tcagttactt tagttattcc
    15661 aata
//
LOCUS       DATRUK010000050        15648 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.050, whole genome shotgun sequence.
ACCESSION   DATRUK010000050 DATRUK010000000
VERSION     DATRUK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15648
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.050"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..573)
                     /gene="recX"
                     /locus_tag="VBE34_001466"
     CDS             complement(<1..573)
                     /gene="recX"
                     /locus_tag="VBE34_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEO7264177.1"
                     /translation="MKITKIEKKKRLYTLELDNTENLYITEDTIVHFMLSKGMIINAE
                     KLENIKKFAQLSYGKNLGLYYISFKQRTEKEVIKYLQEHDIDSKIIPQIIDNLKSENW
                     INDKNYVQSFIQQNLNNGDKGPYVIKQKLLQKGIKSKIIESELKAINFQDLASKISQK
                     LYKKYQNKLPLKALKDKLMQSLTTKGFDYQI"
     gene            672..2027
                     /gene="rlmD"
                     /locus_tag="VBE34_001467"
     CDS             672..2027
                     /gene="rlmD"
                     /locus_tag="VBE34_001467"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO7264178.1"
                     /translation="MNVVLKQRIPLKIKRMGINGEGIGFYKKTLIFVPGALKGEEVFC
                     QISSVRRNFAEAKLLKINKKSKNRVDPACSIYKECGGCQIMHLQYDKQLEFKTDIIRQ
                     ALMKFKPEGYENYEIRKTIGMSEPEHYRAKLQFQVRSFGGNVKAGLYAQGTHRLIDIK
                     DCLVQDRLTQEMINRVAELLGKYKLPIYNERKIAGVRTVMIRRAQASGEVQLIFITSK
                     RLDFDDVVIELVREFPELKTVAVNINASKTSDIYGQITEVIWGQESINEEVLDYGFSL
                     SPRAFYQLNPKQTQILYSEAVKALDVKEDDDLIDAYCGVGTIGLAFAGKVKSVRGMDI
                     IPEAIQDAKENALHMGFTNTHYEAGKAEDIIPRWYSEGFRANALIVDPPRTGLDDKLL
                     NTILKMPPEKMVYVSCNTSTLARDLVTLTKVYHVHYIQSVDMFPHTARTEAVVKLQRK
                     V"
     gene            2295..2546
                     /locus_tag="VBE34_001468"
     CDS             2295..2546
                     /locus_tag="VBE34_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264179.1"
                     /translation="MIINSFDNSRKATINPEDINSPIKGFPKTVITCFARETFNRILE
                     ELPHREIARTSVANLEIPIYELEFKGQKIVLIKRLVMKI"
     gene            2543..2848
                     /locus_tag="VBE34_001469"
     CDS             2543..2848
                     /locus_tag="VBE34_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7264180.1"
                     /translation="MICLHGMNWVDGQLAAYYRLIPEDDKVHLGRVIVNPDFRKKGLG
                     NQLVEYEIKFSEANYPNKPIYAQAQAYLQDFYQSFGFQPISDIYLEDNIPHLDMVLS"
     gene            3037..6737
                     /locus_tag="VBE34_001470"
                     /pseudo
     CDS             3037..6737
                     /locus_tag="VBE34_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            6947..7855
                     /locus_tag="VBE34_001471"
     CDS             6947..7855
                     /locus_tag="VBE34_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7264181.1"
                     /translation="MKTIKDWQINICLATYNGQKYLRQQLDSIIQQGYTDWICLIRDD
                     GSTDDTVAIIKEYVNRDSRFIFINSNDDRKLGSHRSFYELVNYKKADFYVFSDQDDVW
                     KENRLERYLEEAEKFNQELPLLVYSNWTSVDEKLTVLKEHNPATVIQEQIAFNQINGM
                     VIMMNHELAKLWEYRQIGAHDSYVGTLAYAVGNVAYISDSTVLWRRQVGAESLNNYGR
                     QYGVATFWQMINTSFDRASLIFAQVSDKMSLERKLFFSRFIELKNANLIRRIYLLSKL
                     KLRRKSLKETVAMTILLLTGYGKPKA"
     gene            8409..9419
                     /gene="nrdF"
                     /locus_tag="VBE34_001472"
     CDS             8409..9419
                     /gene="nrdF"
                     /locus_tag="VBE34_001472"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO7264182.1"
                     /translation="MQSYYDRSQSPLDYALSEKAFPMRSVNWNKLNDDKDLEVWNRVT
                     QNFWLPEKIPVSNDLNSWRTLDADWQQLITRTFTGLTLLDSVQATVGDIAQIKHSQTD
                     HEQVIYANFAFMVAIHARSYGTIFSTLCTSQQIEEAHEWVVDTESLQARSRILIPFYT
                     GDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGY
                     KYQQKVAKLSVEKQAEMKTFVFDLLYQLIDLEKAYLYELYDGFDLAEDAIRFSIYNAG
                     KFLQNLGYDSPFTEEETRISPEVFAQLSARADENHDFFSGNGSSYIMGITEETLDEDW
                     EF"
     gene            9420..9833
                     /gene="nrdI"
                     /locus_tag="VBE34_001473"
     CDS             9420..9833
                     /gene="nrdI"
                     /locus_tag="VBE34_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO7264183.1"
                     /translation="MDSTVKVVYFSSKSNNTHRFVQKLACSNQRIPSDGSSILVTEDY
                     ILIVPTYAGGGDDTKGAVPKQVVQFLNVRQNREHCQGVISSGNTNFGDTYAIAGPIIA
                     RKLNVPLLHQFELLGTQEDVTRVKELLCQFTRKDK"
     gene            9835..12000
                     /gene="nrdE"
                     /locus_tag="VBE34_001474"
     CDS             9835..12000
                     /gene="nrdE"
                     /locus_tag="VBE34_001474"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO7264184.1"
                     /translation="MTQSDAYLSLNAKTRFRDRTGNYHFTSDKEAVEQYMIEHVEPNT
                     MVFTSLIEKLDYLVSNNYYESDLLKQYNLEFICQIFEHAYAKKFAFLNFMGALKFYNA
                     YALKTEDNRYYLEHYEDRVVMNALFLAAGDEKAAYDLVDDMLANRFQPATPTFLNAGK
                     KRRGEYISCYLLRIEDNMESISRAISTSLQLSKRGGGVALCLTNLREFGAPIKGIKNQ
                     ATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKS
                     LSLGLVIPDITFELAKANKDMALFSPYDIERVYGKPMSDISITEEYETLLANADIRKT
                     FISARKLFQTIAELHFESGYPYILFEDTVNAKNPHKKEGRIVMSNLCSEIAQVNTASQ
                     FSEDLTFTKVGHDVCCNLGSINIARAMDQAADFEKLIANSIRALDRVSRTSDLDSAPS
                     IKKGNAANHAVGLGAMNLHGFLATNHIYYDSQEAIDFTDCFFYAMAYYAFKASNHLAK
                     EKGTFEGFSESSYADGSYFYQYTEQNFEPKTQRVKNLLAEYGLTLPSQEDWRKLVQSI
                     KEIGLANAHLLAVAPTGSISYLSSCTPSLQPVVSPVEVRKEGALGRVYVPAYKIDADN
                     YVYYKKGAYEVGSEAIINIAAAAQKHIDQAISLTLFMTDQATTRDLNKAYIQAFKQKC
                     ASIYYVRVRQDILEGSESYDDMLDDFTSSDLEDCQSCMI"
     gene            12063..15230
                     /locus_tag="VBE34_001475"
     CDS             12063..15230
                     /locus_tag="VBE34_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEO7264185.1"
                     /translation="MTKKHLKTLALALTTVSVVTYSQEVYGLEREESVKQEQTQSASE
                     DDWFEEDNERKTNVSKENSTVDETVSDLFSDGNSNNSSSKTESVVSDPKQVPKAKPEV
                     TQEASNSSNDASKVEVPKQDTASKKETLETSTWEAKDFVTRGDTLVGFSKSGINKLSQ
                     TSHLVLPSHAADGTQLTQVASFAFTPDKKTAIAEYTSRLGENGKPSRLDIDQKEIIDE
                     GEIFNAYQLTKLTIPNGYKSIGQDAFVDNKNIAEVNLPESLETISDYAFAHMSLKQVK
                     LPDNLKVIGELAFFDNQIGGKLYLPRHLIKLAERAFKSNRIQTVEFLGSKLKVIGEAS
                     FQDNNLRNVMLPDGLEKIESEAFTGNPGDEHYNNQVVLRTRTGQNPHQLATENTYVNP
                     DKSLWRATPDMDYTKWLEEDFTYQKNSVTGFSNKGLQKVRRNKNLEIPKQHNGITITE
                     IGDNAFRNVDFQSKTLRKYDLEEIKLPSTIRKIGAFAFQSNNLKSFEASEDLEEIKEG
                     AFMNNRIGTLDLKDKLIKIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGALHLMFIG
                     NKVKTIGEMAFLSNKLESVNLSEQKQLKTIEVQAFSDNALSEVVLPPNLQTIREEAFK
                     RNHLKEVKGSSTLSQITFNAFDQNDGDKRFGKKVVVRTHNNSHMLADGERFIIDPDKL
                     SSTMVDLEKVLKIIEGLDYSTLRQTTQTQFREMTTAGKALLSKSNLRQGEKQKFLQEA
                     QFFLGRVDLDKAIAKAEKALVTKKATKNGHLLERSINKAVLAYNNSAIKKANVKRLEK
                     ELDLLTDLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSD
                     TIGEGQKDAYGNPILNVDEDNEGYHTLAVATLADYEGLYIKDILNSSLDKIKAIRQIP
                     LAKYHRLGIFQAIRNAAAEADRLLPKTPKGYLNEVPNYRKKQMEKNLKPVDYKTPIFN
                     KALPNEKVDGDRAAKGHNINAETNNSVAVTPIRSEQQLHKSQSDVNLPQTSSKNNFIY
                     EILGYVSLCLLFLVTAGKKGKRARK"
     gene            15243..15632
                     /locus_tag="VBE34_001476"
     CDS             15243..15632
                     /locus_tag="VBE34_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEO7264186.1"
                     /translation="MITQEMKEIINSQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGTYYEAAKKWAEGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEIT"
BASE COUNT         5379 a         2352 c         3026 g         4891 t
ORIGIN      
        1 tatctgataa tcaaatcctt ttgtagttaa gctctgcatc aatttatctt ttaatgcttt
       61 tagtggaagt ttgttttgat attttttata gagtttttgg cttattttgg atgctaagtc
      121 ttgaaagttt atagctttaa gttcagattc aattatttta cttttgatac ctttttgcaa
      181 taatttttgt tttatcacgt atggcccctt atcgccatta tttagatttt gctgtatgaa
      241 agattgcacg tagtttttat cgtttatcca attctcactt tttagattat caataatttg
      301 tggaataatt ttagaatcaa tatcatgttc ttgaagatat ttaataactt ctttctcggt
      361 tctctgcttg aaagaaatat aataaagacc tagattttta ccgtatgata gttgggcaaa
      421 ttttttaata ttttcaagtt tctcggcatt aattatcatc cctttactta acataaaatg
      481 aacgatagta tcttctgtta tatataaatt ttcggtattg tctagctcta gagtgtaaag
      541 tctttttttc ttttcgattt tggttatttt catagttttt attatagcaa aaatgataaa
      601 atagtaaaaa tgagtctttt tttaaatgtt atggatttat agtattttct tagttaggga
      661 gtaatgaact gatgaatgtg gttttaaaac agcgaattcc tttaaaaatt aaacgaatgg
      721 gaattaatgg tgaaggaata ggtttttata aaaaaacact aatctttgtt ccaggggctc
      781 tcaagggaga agaggtgttt tgtcagattt cttctgttag acgtaatttt gcagaagcta
      841 aactgttaaa gataaataaa aagtctaaaa atagggtaga tcccgcttgt tctatttata
      901 aagaatgtgg gggatgccaa ataatgcacc ttcaatatga taagcagcta gagtttaaaa
      961 cggatattat tcgacaggct ctaatgaaat ttaaaccaga gggatatgag aattatgaaa
     1021 tccgtaaaac gataggtatg tcggaacctg aacactatcg tgctaaatta caatttcaag
     1081 tgcgttcgtt tggagggaat gtaaaggcag gtttatatgc acaagggaca catcgattga
     1141 ttgatatcaa agattgttta gtgcaagata ggcttactca ggaaatgata aatcgagttg
     1201 cagaactttt aggaaaatat aaattgccta tttataatga acgtaaaata gctggtgttc
     1261 gtactgttat gattcgacga gcacaagcga gtggagaggt tcaactgatt tttatcacaa
     1321 gtaaaagact tgattttgat gatgttgtta ttgaattagt tcgagagttt cctgagttaa
     1381 agacagttgc tgtaaatatc aacgctagta aaacaagtga tatctatggt caaataacag
     1441 aagttatttg gggtcaagaa tccatcaatg aagaagtatt agattatggt ttttcgttat
     1501 caccacgtgc tttttatcaa cttaatccta aacaaacaca aattttatat agtgaggcag
     1561 tcaaggcctt agatgtgaag gaagatgatg atttaattga cgcttattgt ggagtaggaa
     1621 cgattggact tgcttttgct ggaaaagtta aaagtgttcg tggaatggat attatccctg
     1681 aggctattca ggatgctaaa gagaacgcac ttcatatggg ttttactaat acacattacg
     1741 aagctggaaa ggcagaggat attatcccta ggtggtatag tgagggattt cgcgctaatg
     1801 cgttaatcgt ggatcctcct cgcacaggct tagatgataa attgctaaat actatactaa
     1861 aaatgcctcc agaaaaaatg gtttacgttt cttgtaatac atctacgctt gctagagact
     1921 tagtaacatt aacaaaagta taccatgttc actacatcca atctgttgac atgtttccac
     1981 atacagcaag gaccgaggct gtagtgaaac tacaacgtaa ggtataataa agttttctaa
     2041 tctaaaaata agagtatttt tgggtattct tatttttaga tagtaatggt gagatattaa
     2101 tatagcatag tataaaacac atggtttaag ctatttgata cggaaattta ccttagaaat
     2161 ggtaaataat tccattaagt atattcatct ttgcaaattt gaccttgcct tttaagtttc
     2221 attgggttaa aatagaaaaa atatttttaa caagatgata gaacatcgta aattaaggtt
     2281 taaaggaaat gcttatgata ataaattcat tcgataatag tagaaaagcg actattaatc
     2341 ctgaagatat taattcacca ataaaaggtt ttccaaagac tgttattacc tgttttgcgc
     2401 gggagacttt caatcgcatt ttagaagaat tgcctcatcg tgaaattgct agaactagtg
     2461 tagcaaactt agaaattccc atttatgaat tagaatttaa aggacaaaag attgtcctta
     2521 tcaagaggtt ggtgatgaag atttgatttg tttgcatggg atgaattggg tagatggtca
     2581 attggcagct tattatagat tgataccaga agatgataag gtgcatttgg gacgtgtgat
     2641 tgttaatcca gactttagaa aaaagggatt aggaaatcaa ttagtggaat atgagattaa
     2701 atttagcgag gcaaattacc ctaataaacc tatttatgct caggcccaag cttatttaca
     2761 agatttttat caatctttcg gatttcaacc tatctcagat atctatctag aagataatat
     2821 tcctcatctt gatatggttt tatcataaat acatactatt taaactatca attaaaaggt
     2881 ctctcagtat tactgagaga tcttttaatt aacaaaaaat taaaaaaatt taaattaaaa
     2941 aataaaaata aaatttatcg ctaattattc tgttgctaat acattaatta tgttgtaata
     3001 tagtatgcat actgattttt ggaggaaata ggaaaagtgg ataaacatca ctcaaaaaag
     3061 gctattttaa agttaacact tataacaact agtattttat taatgcatag caatcaagtg
     3121 aatgcagagg agcaagaatt aaaaaaccaa gagcaatcac ctgtaattgc taatgttgct
     3181 caacagccat cgccatcggt aactactaat attgttgaaa aaacatctgt aacagctgct
     3241 tctgctagta atacagtgaa agaaatgggt gatacatctg taaaaaatga caaaacagaa
     3301 gatgaattat tagaagagtt atctaaaaac cttgatacgt ctaatttggg ggctgatctt
     3361 gaagaagaat atccctctaa accagagaca accaacaata aagaaagcaa tgtagtaaca
     3421 aatgcttcaa ctgcaatagc acagaaagtt ccctcagcat atgaagaggt gaagtcagaa
     3481 agcaagtcat cgcttgctgt tcttgataca tctaaaataa caaaattaca agccacaacc
     3541 caaagaggaa agggaaatgt agtagctatt attgatactg gctttgatat taaccatgat
     3601 atttttcgtt tagatagccc aaaagatgat aagcacagct ttaaaactaa ggcagaattt
     3661 gaggaattaa aagcaaaaca taatatcact tatgggaaat gggttaacga taagattgtt
     3721 tttgcacata actacgccaa caatacagaa acggtggctg atattgcagc agctatgaaa
     3781 gatggttatg ggtcagaagc aaagaatatt ttgcatggta cacacgttgc tggtattttt
     3841 gtaggtaata gtaaacgtcc agcaatcaat agtcttcttt tagaaggtgc agcgccaaat
     3901 gctcaagtct tattaatgcg tattccagat aaaattgatt cggacaaatt tggagaagca
     3961 tatgctaaag caatcataga cgctgttaat ctaggagcaa aaacgattaa tatgagcctg
     4021 ggaaaaacgg ctgattcttt aattgctctc aatgataaag ttaaattagc acttaaatta
     4081 gcttctgaga agggcgttgc agttgttgtg gctgccggaa atgaaggtgc atttggtatg
     4141 gattatagca aaccattatc aactaatcct gactacggta cggttaatag tccagctatt
     4201 tctgaagata ctttgagtgt tgctagctat gaatcactta aaactatcag tgaggtcgtt
     4261 gaaacaacta ttgaaggtaa gttagttaag ttgccgattg tgacttctaa accttttgac
     4321 aaaggtaagg cctacgatgt ggtttatgcc aattatggtg caaaaaagat tttgaaggta
     4381 aggactttaa aggtaagatt gcattaattg agcgtggtgg tggacttgat tttatgacta
     4441 aaatcactca tgctacaaat gcaggtgttg ttggtatcgt tatttttaac gatcaagaaa
     4501 aacgtggaaa ttttctaatt ccttaccgtg aattacctgt gggggttatt agtaaagtag
     4561 atggcgagcg tataaaaaat acttcaagtc agttaacatt taaccagagt tttgaagtag
     4621 ttgatagcca aggtggcaat cgtatgctgg aacaatcaag ttggggcgtg acagctgaag
     4681 gagcaatcaa gcctgatgta acagcttctg gctttgaaat ttattcttca acctataata
     4741 atcaatacta aacaatgtct ggtacaagta tggcttcacc acatgttgca ggattaatga
     4801 caatgcttca aagtcatttg gctgagaaat ataaagggat gaatttagat tctaaaaaat
     4861 tgctagaatt gtctaaaaac atcctcatga gctcagcaac agcattatat agtgaagagg
     4921 ataaggcgtt ttattcacca cgtcagcaag gtgcaggtgt agttgatgct gaaaaagcta
     4981 tccaagctca atattatgtt actggaaacg atggcaaagt taaaattaat ctcaaacgag
     5041 agggagataa atttgatatc acagttacaa ttcataaact tgtagaaggt gtcaaagaat
     5101 tgtattatca agctaatgta gcaacagaac aagtaaataa aggtaaattt gcccttaaac
     5161 cacaagcctt gctagatact aattggcaga aagtaattct tcgtgataaa gaaacacaag
     5221 ttcgatttac tattgatgct agtcaattta gtcagaaatt aaaagaacag atggcaaatg
     5281 gttatttctt agaaggtttt gtacgtttta aagaagccaa ggatagtaat caggagttaa
     5341 tgagtattcc ttttgtagga tttaatggtg attttgcgaa cttacaagca cttgaaacac
     5401 cgatttataa gacgctttct aaaggtagtt tctactataa accaaatgat acaactcata
     5461 aagaccaatt ggagtacaat gaatcagctc cttttgaaag caacaactat actgccttgt
     5521 taacacaatc agcgtcttgg ggctatgttg attatgtcaa aaatggtggg gagttagaat
     5581 tagcaccgga gagtccaaaa agaattattt taggaacttt tgagaataag gttgaggata
     5641 aaacaattca tcttttggaa agagatgcag cgaataatcc atattttgcc atttctccaa
     5701 ataaagatgg aaatagggac gaaatcactc cccaggcaac tttcttaaga aatgttaagg
     5761 atatttctgc tcaagttcta gatcaaaatg gaaatgttat ttggcaaagt aaggttttac
     5821 catcttatcg taaaaatttc cataataatc caaagcaaag tgatggtcat tatcgtatgg
     5881 atgctcttca gtggagtggt ttagataagg atggcaaagt tgtagcagat ggtttttata
     5941 cttatcgctt acgttacaca ccagtagcag aaggagcaaa tagtcaggag tcagacttta
     6001 aagttcaagt aagtactaag tcaccaaatc ttccttcacg agctcagttt gatgaaacta
     6061 atcgaacatt aagcttagcc atgcctaagg aaagtagtta tgttcctaca tatcgtttac
     6121 aattagtttt atctcatgtt gtaaaagatg aagaatatgg ggatgagact tcttaccatt
     6181 atttccatat agatcaagaa ggtaaagtga cacttcctaa aacggttaag ataggagaga
     6241 gtgaggttgc ggtagaccct aaggccttga cacttgttgt ggaagataaa gctggtaatt
     6301 ttgcaacggt aaaattgtct gacctcttga ataaggcagt agtatcagag aaagaaaacg
     6361 ctatagtaat ttctaacagt ttcaaatatt ttgataactt gaagaaagaa cctatgttta
     6421 tttctaaaga aggaaaagta gtaaacaaga atctagaaga aataacatta gttaagcctc
     6481 aaactacagt tactactcaa tcattgtcta aagaaataac taaatcagga aatgagaaag
     6541 tcctcacttc tacaaacaat aatagtagca gagtagctaa gatcatatca cctaaacata
     6601 acggggattc tgttaaccat accttaccta gtacatcaga tagagcaacg aatggtctat
     6661 ttgttggcac tttggcattg ttatctagtt tacttcttta cttgaaaccc aaaaagacta
     6721 aaaataatag taaataaaac taagtaaact actcggtaat atgatgaaaa gttcagttta
     6781 acattatgtt aagctggact tttagggtat tgactcttat ttttgattaa ttttatattg
     6841 attaacggca tataatatta taatattata attacgatta gtatgtgata ctgttcgctt
     6901 tgtaagcttt cataagatga actagcctta aggagtttgg atagcaatga aaacgataaa
     6961 agattggcaa ataaatattt gcttagcgac ctataatggt caaaaatacc ttcgccagca
     7021 attagatagc ataatacaac aaggttatac tgactggatt tgtttaataa gggacgatgg
     7081 atctactgat gatacagtag ctattattaa agaatatgtt aatagagatt cacgttttat
     7141 atttattaat tctaacgatg atagaaagtt aggaagtcac cgcagttttt acgagttggt
     7201 taactataaa aaagcagatt tttatgtttt ctcagaccag gatgatgttt ggaaagaaaa
     7261 tcgtttagaa cgttaccttg aagaagcaga gaaatttaac caagaattac cattgcttgt
     7321 ttattcgaac tggacttctg tagatgaaaa attaacagta ttgaaagaac ataatccagc
     7381 aacagttatt caagaacaaa ttgcctttaa ccaaataaat ggtatggtta tcatgatgaa
     7441 tcatgagtta gcaaagcttt gggaataccg tcaaattggc gcccatgatt cttatgttgg
     7501 aactctggct tacgccgttg gaaatgtggc ttatatttct gacagtacag tcctttggcg
     7561 tagacaagta ggggcagagt cgctaaataa ttacgggaga caatatgggg tggctacgtt
     7621 ttggcagatg ataaatactt cttttgatcg tgcttcgctt atttttgctc aagtatccga
     7681 taaaatgtcc ctggagcgta aactcttctt tagtcgtttt atagaattaa aaaatgccaa
     7741 tctaataaga aggatttatc ttttatcaaa attaaaactt cgtcggaaat cattgaaaga
     7801 aacagttgct atgactatcc tacttttaac aggttacggt aaaccaaaag cataattttt
     7861 ttggttgaag gaaatcaagt tgagttgtag ttaaaaatat agaaaatatt tatttttcaa
     7921 aaagttgttg acacttaacg aaattagtgt ataataatat taaagaacaa ggtagaacct
     7981 tggctcgcat taatttcggg ttttggaaaa ggagtagcaa tcgctactcc ttttttgttt
     8041 tacacaggaa aagtatgaag gaaaagcctt ttttcttata aaaattaagc ttgcttaatt
     8101 ttttaatttt tatattcttt acattatgat gaaaacacaa gatatagtgg tgatatatgt
     8161 tgaaagacac aaaactttgt gttagtatac tgtttaagca gatccttaat aaaggtaacc
     8221 gacaaaaaag tttaacgtga cttaacaaaa cagtggtttt gaagcaagag ttagcttctt
     8281 accagaataa aaaaacatca agaagaacat tccaatagtt gtgtgatttt aacaaaaatc
     8341 cagtcctctt gggaggcttt tttggtgctt aaaactcgct taattttaat atttaggaga
     8401 ctagtttaat gcaatcatac tatgatcgtt ctcaatcacc acttgactat gccttgtcag
     8461 aaaaagcttt tcctatgcgt tctgttaatt ggaataagct aaacgatgac aaagacttag
     8521 aggtatggaa cagagtaact caaaatttct ggttacctga aaagatccca gtttctaatg
     8581 accttaattc ttggagaaca ttggatgctg attggcagca attaattact cggactttta
     8641 ctggtctaac cttattagat tctgtgcagg ctactgttgg tgatattgct cagattaagc
     8701 attctcagac agatcatgaa caagttattt atgctaactt tgcctttatg gtagctattc
     8761 atgcgcgatc ttatggaacg attttttcaa ccttatgcac tagccaacaa attgaggaag
     8821 cacatgaatg ggtagtggat actgaaagtc tacaagctcg atcacgtata cttattcctt
     8881 tttatactgg tgacgatcct ttgaaatcta aggttgctgc agccatgatg ccaggatttt
     8941 tgttatatgg tggcttttac cttccttttt acctttcagc cagaggaaaa ttaccaaata
     9001 cgtctgatat tattcgttta attttacgag ataaggtaat tcataactat tacagtggct
     9061 ataagtatca acagaaagtt gctaaattat ctgtagaaaa gcaagccgaa atgaagacat
     9121 ttgtctttga cctcctctat cagttgattg atttagaaaa agcctatctt tacgaactat
     9181 atgatggttt tgatttggct gaagatgcaa tcagatttag catttacaat gctggtaaat
     9241 ttttacaaaa tttaggatat gattctccat ttacagaaga agagactcgt atatctcctg
     9301 aggtttttgc acaactttcg gcgcgtgcag atgaaaatca tgatttcttc tcaggaaacg
     9361 gctcttcata tattatgggg attacagaag aaacattgga tgaagattgg gagttttaaa
     9421 tggatagtac agtgaaggtg gtctattttt catcgaaatc taacaatacc catcgttttg
     9481 ttcaaaagtt ggcttgttct aaccagcgta ttccatcaga tggtagtagt atattggtta
     9541 ctgaggatta tattttgatt gtgcctactt acgctggagg aggagatgat accaaaggcg
     9601 cagttccaaa acaagttgta cagtttttaa atgttcgaca aaatcgggaa cattgccaag
     9661 gggttatttc ttctggaaat accaattttg gtgacactta tgccattgct ggacctatta
     9721 ttgctcgaaa attaaatgtt cctttgcttc atcaatttga attactggga acacaagaag
     9781 atgttacaag ggtaaaggag ttactttgcc aattcactag aaaggataaa taagatgaca
     9841 caatcagatg catatctctc gttgaacgcg aagacacgct ttagagatcg cacaggtaat
     9901 tatcatttta cttcggataa agaggctgtt gaacaatata tgatagaaca tgttgaacct
     9961 aatacgatgg tgttcacatc actaattgaa aagctagatt atttggtttc taataactac
    10021 tatgaatcgg accttctaaa acaatataac cttgagttta tttgccaaat ttttgagcat
    10081 gcatacgcta agaaatttgc ttttctaaat tttatggggg ctttaaaatt ttataatgct
    10141 tatgctctta aaacagaaga caatcgttat tatttggaac actatgaaga tcgtgttgta
    10201 atgaatgctc tgtttttagc tgctggtgat gaaaaagctg cctatgacct agtagatgac
    10261 atgctagcaa atcgctttca accagcaaca cctacctttt taaatgcagg gaaaaaacgt
    10321 cgtggtgaat atatctcttg ctatttgttg cgcattgaag acaatatgga atctatttct
    10381 cgtgctatta gtacaagcct tcagttatca aaacgaggtg gtggggtagc actttgctta
    10441 accaacttac gggaatttgg tgctccaata aaaggtatta aaaatcaagc tactggtatt
    10501 gtacctgtca tgaaattact agaagattcg ttttcttacg ccaatcaatt aggacaaaga
    10561 caaggagctg gtgcagttta cttacacgct caccatcctg aagtattaac gtttttagat
    10621 actaaacgtg agaatgccga tgagaaaatt cgaattaaat ctctttccct aggacttgtt
    10681 attcctgata ttacttttga attggctaag gcaaataaag atatggcctt gttttcaccg
    10741 tatgacattg agcgagtata tggtaaacca atgtctgata tctctatcac agaagaatat
    10801 gaaactttat tagccaatgc ggatattcgt aaaaccttta ttagtgcacg aaaactattt
    10861 caaacgatag cagagcttca ttttgaatct ggttatcctt atatcttatt tgaagatact
    10921 gtaaatgcta aaaaccctca caaaaaagag ggacgaattg ttatgtctaa tttatgttct
    10981 gagattgcac aagttaatac agctagtcaa ttttcagaag acctgacttt cactaaggtt
    11041 ggacatgatg tttgttgtaa tttaggttct attaatattg cgcgtgctat ggatcaagcg
    11101 gcggattttg aaaaattaat tgctaatagc attagagccc ttgatcgtgt atctcgaact
    11161 tctgatttag atagtgctcc atctattaaa aaaggtaatg cagctaatca tgctgttggt
    11221 ttaggtgcta tgaatttgca tggatttttg gcgactaacc atatttatta cgattctcaa
    11281 gaggctattg attttacaga ttgcttcttt tacgctatgg cttattatgc ctttaaagct
    11341 tctaaccatt tagcaaaaga aaaaggaacc tttgagggct tctcggaatc aagttatgct
    11401 gatggcagtt atttctatca atataccgaa caaaactttg agccaaaaac acagcgtgtt
    11461 aaaaatctac tagctgagta tggtctgact ctaccaagcc aagaggattg gagaaagctt
    11521 gtgcaaagta ttaaagagat aggattagct aatgcgcatt tattagctgt tgctccgaca
    11581 gggtcaatca gttatctttc ttcttgtact ccgagccttc aaccggttgt atcacctgtc
    11641 gaagtacgca aggaaggagc actggggagg gtttatgtac ctgcttataa gattgatgca
    11701 gataattatg tctactacaa aaaaggagct tatgaagtgg gatctgaggc gattatcaat
    11761 attgcagctg ccgctcaaaa acacattgat caagctattt cgttaacgct tttcatgaca
    11821 gatcaagcaa ctacgcgaga tttaaataaa gcctatattc aagcatttaa acaaaaatgt
    11881 gcctctattt attatgtacg agtgagacag gacatcctag aaggtagcga gagttatgat
    11941 gatatgctgg atgatttcac ttcatcggac ttagaagact gtcaatcctg catgatttaa
    12001 ctaaatatac tcaatcagaa gattaagaga aatatattgg cttttagaaa ggctttattg
    12061 taatgacaaa aaaacatctt aaaacgcttg ccttggcact tactacagta tcagtagtga
    12121 catacagcca ggaggtatat ggattagaaa gagaggaatc ggtcaaacaa gaacaaaccc
    12181 agtcagcttc agaagatgat tggttcgaag aagataatga gaggaaaaca aatgtttcta
    12241 aagagaattc tactgttgat gaaacagtta gtgatttatt ttctgatgga aatagtaata
    12301 actctagttc taaaaccgag tcagtggtaa gtgaccctaa acaagtcccc aaagcaaaac
    12361 cagaggttac acaagaagca agcaattcta gtaatgatgc tagcaaagta gaagtaccaa
    12421 aacaggatac agcttcaaaa aaggaaactc tagaaacatc aacttgggag gcaaaagatt
    12481 tcgtaactag aggggatact ttagtaggtt tttcaaaatc tggaattaat aagttatctc
    12541 aaacatcaca cttggtttta ccaagtcatg cagcagatgg aactcaattg acacaagtag
    12601 ctagctttgc ttttactcca gataaaaaga cggccattgc agaatataca agtaggctag
    12661 gagaaaatgg gaaaccgagt cgtttagata ttgatcagaa ggaaattatt gatgagggag
    12721 aaatatttaa tgcttaccag ttgactaagc ttactattcc aaatggttat aagtctattg
    12781 gtcaagatgc ttttgtggac aataagaata ttgctgaggt taaccttcct gagagtctcg
    12841 agactatttc agactatgct tttgctcaca tgtctttaaa acaagtaaag ttaccagata
    12901 acctaaaggt cattggagaa ttagcttttt ttgataatca gattggtggt aagctttact
    12961 tgccacgtca cttgataaaa ttagcagaac gcgctttcaa atctaatcgt attcaaacag
    13021 ttgaattttt gggaagtaag cttaaggtta taggagaagc aagttttcaa gataataatc
    13081 tgaggaatgt tatgcttccg gatggacttg aaaaaataga atcagaagct tttacaggaa
    13141 atccaggaga tgaacattac aacaatcagg ttgtattgcg cacaaggaca ggccaaaatc
    13201 cacatcaact tgcgactgag aatacttacg tcaatccgga caaatcattg tggcgtgcaa
    13261 cacctgatat ggattatacc aaatggttag aggaagattt tacctatcaa aaaaatagtg
    13321 ttacaggttt ttcaaataaa ggcttacaaa aggtaagacg taataaaaac ttagaaattc
    13381 caaaacaaca caatggtatt actattactg aaattggtga taacgctttt cgcaatgttg
    13441 attttcaaag taaaacttta cgtaaatatg atttggaaga aataaagctc ccctcaacta
    13501 ttcggaaaat aggtgctttt gcttttcaat ctaataactt gaaatccttt gaagcaagtg
    13561 aagatttaga agagattaaa gagggagcct ttatgaataa tcgtattgga actctagact
    13621 tgaaagacaa acttatcaaa ataggtgatg ctgctttcca tattaatcat atttatgcca
    13681 ttgttcttcc agaatctgta caagaaatag gacgttcagc ttttcgacaa aatggtgcgc
    13741 ttcaccttat gtttatcgga aataaggtta aaacaattgg tgaaatggct tttttatcca
    13801 ataaactgga aagtgtaaat ctctctgagc aaaaacaatt aaagacaatt gaggtccaag
    13861 ctttttcgga taatgccctt agtgaagtag tcttaccgcc aaatttacag actattcgtg
    13921 aagaggcttt caaaaggaat catttgaaag aagtgaaggg ttcatctaca ttatctcaga
    13981 ttacttttaa tgcttttgat caaaatgatg gggacaaacg ctttggtaag aaagtggttg
    14041 ttaggacaca taataattct catatgttag cagatggtga gcgttttatc attgatccag
    14101 ataagctatc ttctacaatg gtagaccttg aaaaggtttt aaaaataatc gaaggtttag
    14161 attactctac attacgtcag actactcaaa ctcagtttag agaaatgact actgcaggta
    14221 aagcgttgtt atcaaaatct aacctccgac aaggagaaaa acaaaaattc cttcaagaag
    14281 cacaattttt ccttggtcgc gttgatttgg ataaagccat agctaaagct gagaaggctt
    14341 tagtgaccaa gaaggcaaca aagaatggtc atttgcttga gaggagtatt aacaaagcgg
    14401 tattagctta taataatagt gctattaaaa aagctaatgt taagcgcttg gaaaaagagt
    14461 tagacttgct gacagattta gtcgagggaa aaggaccatt agcgcaagct acaatggtac
    14521 aaggagttta tttattaaag acgcctttac cattgccaga atattatatc ggattgaacg
    14581 tttattttga caagtctgga aaattgattt atgcacttga tatgagtgat actattggcg
    14641 agggacaaaa agatgcatat ggtaatccta tattaaatgt tgacgaggat aatgaaggtt
    14701 atcatacctt ggcagttgcc actttagctg attatgaagg tctttatatt aaagatattt
    14761 taaatagttc ccttgataag attaaagcaa tacgccagat tcctttggca aaatatcata
    14821 gattaggaat tttccaagct atccgaaatg cagcggcaga agcagaccga ttgcttccta
    14881 agacacctaa ggggtaccta aatgaagtcc caaattatcg taaaaaacaa atggagaaaa
    14941 atttaaaacc agttgattat aaaacgccga tttttaataa ggctttacct aatgaaaagg
    15001 tagacggtga tagagcggct aaaggtcata atataaatgc ggagactaat aattctgtag
    15061 ctgtaacacc aataaggtcc gagcagcaat tacataagtc acagtctgat gtaaatttac
    15121 ctcaaacaag ttctaaaaat aattttatat acgagattct aggatacgtt agtttatgtt
    15181 tgcttttcct agtaactgct gggaaaaaag gaaaacgagc aagaaaataa ggagaaatta
    15241 atatgattac acaagaaatg aaagagatta ttaatagtca gttagcaatg gtcgcgacgg
    15301 ttgatgctaa aggtcaaccc aatattggcc caaaacgttc tatgcgtctc tgggatgata
    15361 aaacgtttat ttataatgaa aatacagatg gtcaaactcg tattaacatt gaagataatg
    15421 ggaaaattga aattgccttt gttgatcgtg aacgtctttt aggttacagg tttgtgggaa
    15481 ctgctgaaat tcaaacagaa ggtacttatt acgaagcagc gaaaaaatgg gcagaaggac
    15541 gcatgggggt tccgaaagca gttggtatta ttcatgtcga gcgtattttt aatttacaat
    15601 ctggtgctaa tgcaggtaaa gaaatcactt aatataaaaa agcctcaa
//
LOCUS       DATRUK010000051        15521 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.051, whole genome shotgun sequence.
ACCESSION   DATRUK010000051 DATRUK010000000
VERSION     DATRUK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15521
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.051"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            146..571
                     /gene="rplK"
                     /locus_tag="VBE34_001477"
     CDS             146..571
                     /gene="rplK"
                     /locus_tag="VBE34_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEO7264187.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGEPNKTKVATITR
                     AQVQEIAETKMPDLNAANLESAMRMIEGTARSMGFTVTD"
     gene            675..1364
                     /gene="rplA"
                     /locus_tag="VBE34_001478"
     CDS             675..1364
                     /gene="rplA"
                     /locus_tag="VBE34_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEO7264188.1"
                     /translation="MAKKSKNLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     SYNLNIDVKKADQQIRGAMVLPAGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KIQGGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDDAKLVDNFKAFNDVIVKAKPATAKGTYITNLSITTTQ
                     GVGIKVDPNSL"
     gene            1753..3370
                     /gene="nikA"
                     /locus_tag="VBE34_001479"
                     /pseudo
     CDS             1753..3370
                     /gene="nikA"
                     /locus_tag="VBE34_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
     gene            3357..4301
                     /locus_tag="VBE34_001480"
     CDS             3357..4301
                     /locus_tag="VBE34_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7264189.1"
                     /translation="MYLSSSIIKKILSAFLALFFISLLTFILIKLSTVNPAENYLRLS
                     KISVSPEALKEAEHYLGLDKPLWKQYWLWFQKALTGDFGYSYVLRLPVLDLVLQRFLA
                     TLFLGTSAFLLIVTISTPLGVWAGLHESARSDHLIRFLSFSSVSMPNFWVAYLLMLLF
                     SAKLNLLPVSGGNDLQSLILPSITLSFSTVGQYIALIRKAISQENRSLNVENARLRGV
                     KERYIVTHHLLRNALPAIMTALSLTWVYLLTGSIIVEEIFSWNGIGRLFVTSLRTSDL
                     PVIQACMLIFGTLFLANNFMTQCFMNWVDPRLRKSREK"
     gene            4301..5107
                     /locus_tag="VBE34_001481"
     CDS             4301..5107
                     /locus_tag="VBE34_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7264190.1"
                     /translation="MSKRIYFSSSLLVLLVISAIFAPILSSFDPQYVDLSQKLLAPNN
                     VHLLGTDQLGRDVLSRLLYGARYSLFLAIIISLLELTIGMFVGLIVGWYQGKLENLFL
                     WIANIILAFPSFLLSLATVGILGHGLGNLIFAIVFVEWVYYAKLMTNLVKSAKKEPYV
                     INAQIMGLSVWHILRKHIFPFVYQPILVMVLMNIGNIILMISGFSFLGIGVQPNVTEW
                     GMMLHDARGYFRTATWMMLSPGIAIFLTVFSFNTLGDAIDKKDWKRQWNS"
     gene            5095..5883
                     /locus_tag="VBE34_001482"
     CDS             5095..5883
                     /locus_tag="VBE34_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7264191.1"
                     /translation="MEQLEIRKLSLQIGEVPVLRDFSCKIDMGESLTIIGESGSGKTL
                     LAKLLVGHIPQGMTVRGNIFFKGVDLGKLTVKQWQKLRGRDIAYLVQNPMSMFNPFQK
                     IEAHILETILSHEKCSKRVALSKALEWMKRLNLDDAISLLKKYPFELSGGMLQRIMLA
                     TILSLDPQVIILDEPTSAVDCHNCSTISAILQELQNNGKTLITVTHDYQLARDLGGQL
                     LVISEGEVVEQGQTQAILSNPQHNYTKALTVQMEYEGDILNVGL"
     gene            5870..6551
                     /locus_tag="VBE34_001483"
                     /pseudo
     CDS             5870..6551
                     /locus_tag="VBE34_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            6672..7400
                     /locus_tag="VBE34_001484"
     CDS             6672..7400
                     /locus_tag="VBE34_001484"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEO7264192.1"
                     /translation="MEPKYQRILIKLSGEALAGDKGVGIDIPTVQSIAKEIAEVHNSG
                     VQIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQYGVDTRVQT
                     AIPMQTVAEPYVRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEAEAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHVEVIKRGLKIMDATASTISMDNDIDLVVFNMN
                     ETGNIKRVVLGEQIGTTVSNKASE"
     gene            7416..7973
                     /gene="frr"
                     /locus_tag="VBE34_001485"
     CDS             7416..7973
                     /gene="frr"
                     /locus_tag="VBE34_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEO7264193.1"
                     /translation="MTKEIVTKAQERFEQSHQSLSREFAGIRAGRANASLLDRIQVEY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERAINESDLGINPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKIAIRNIRRDAMDEAKKQEKNKEITEDDLKSLEKDI
                     QKATDDAVKHIDEMTANKEKELLEV"
     gene            8091..8945
                     /gene="cvfB"
                     /locus_tag="VBE34_001486"
     CDS             8091..8945
                     /gene="cvfB"
                     /locus_tag="VBE34_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEO7264194.1"
                     /translation="MNTLLATVITGLVTDENKDFYFIQKDGFTFALSKSEGEHHIGEM
                     VKGFAYTDMQQKARLTTKETFATRDHYGWGTVTEVRKDLGVFLDTGLPDKQVVVSLDV
                     LPELKELWPKKGDRLYVCLDVDKKDRLWALPADPEVFQRMATPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYSEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKKIKQ
                     DQLGTELL"
     gene            9083..9592
                     /gene="msrA"
                     /locus_tag="VBE34_001487"
     CDS             9083..9592
                     /gene="msrA"
                     /locus_tag="VBE34_001487"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEO7264195.1"
                     /translation="MERAIFAGGCFWCMVQPFEELDGIESVLSGYTGGHVENPTYKEV
                     CSKTTGHTEAVEIIFNPEKISYADLVELYWAQTDPTDAFGQFEDRGDNYRPVIFYENE
                     EQRQIAQKSKDKLQASGRFDRPIVTSIEPADTFYPAEDYHQAFYRTNPARYALSSARR
                     HAFLEENWH"
     gene            9589..9804
                     /locus_tag="VBE34_001488"
     CDS             9589..9804
                     /locus_tag="VBE34_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEO7264196.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADYAFDETTFPKHSSDFETV
                     SRYLEDEASFSFNLTDFDDIWEDYLNH"
     gene            9961..11733
                     /locus_tag="VBE34_001489"
     CDS             9961..11733
                     /locus_tag="VBE34_001489"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEO7264197.1"
                     /translation="MRYTNGNFEAFARPRKPEGVDKKSAYIVGSGLAGLAAAVFLIRD
                     GQMDGQRIHIFEELPLSGGSLDGVKRPDIGFVTRGGREMENHFECMWDMYRSIPSLEV
                     PDASYLDEFYWLDKDDPNSSNCRLIHKQGNRLESDGDFTLGTHSKELVKLVMETEESL
                     GAKTIEEVFSKEFFESNFWTYWATMFAFEKWHSAIEMRRYAMRFIHHIGGLPDFTSLK
                     FNKYNQYDSMVKPIISYLESHNVDVQFDSKVTNISVDFKNGQKLAKAIHLTVGGEAKT
                     IDLTPNDFVFVTNGSITESTNYGSHDTVAKPNTDLGGSWNLWENLAAQSDEFGHPKVF
                     YKDIPKESWFVSATATIKDPAIEPYIERLTHRDLHDGKVNTGGIVTVTDSNWMMSFAI
                     HRQPHFKEQKENETIVWIYGLYSNVEGNYIKKPIEECTGREITEEWLYHLGVPEMKIH
                     DLSDKQYVSTVPVYMPYITSYFMPRVKGDRPDVIPQGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYTFLNIERGVPEVFNSAFDIRVLLQSLYYLNDKKSVEDMDLPIPAL
                     MRKVGMKKIRGTYLEELLREAHLL"
     gene            11857..12843
                     /locus_tag="VBE34_001490"
     CDS             11857..12843
                     /locus_tag="VBE34_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEO7264198.1"
                     /translation="MQEYSIEITLQHPDDMMSLFGSNERHLKLIEENLDVIIHARTER
                     VQVLGDSEEAVETARLTIEALLVLVNRGMTVNTSDVVTALSMAQNGSIDKFVALYEEE
                     IIKDSYGKPIRVKTLGQKIYVDSVKNHDVVFGIGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKEQTSRLMEREIIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDVSQIDLPKNVKS
                     GLIDAVEKLRNIKKIDFIHLSAKDVVRHPVVAEIINAYSDSESSHKLQSDKD"
     gene            complement(12934..13737)
                     /locus_tag="VBE34_001491"
     CDS             complement(12934..13737)
                     /locus_tag="VBE34_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HEO7264199.1"
                     /translation="MDHFTKLWQDFSKLPNVVAIALGGSRSGDSFDQSSDYDLYVYCA
                     ATPDITSRKRILNKHCHYIELNNHYWELEDNGTLNDGTDIDILYRNIDNFLSDLEDVV
                     EHHNSRIGYTTCFWHNLINCQILYDPENQLQSLKERFEVSYPSQLQKQIIIQNRNLLT
                     GKLPSYDKQIIKALKRQDFVSTHHRTTAFLDSYFDIIFALNKLTHPGEKRMISYAKKN
                     ATLLPKHFEENIIKLCHHNSNEHTVKETLNDIIMHLDVMLKENFQHFIG"
     gene            13929..14405
                     /locus_tag="VBE34_001492"
     CDS             13929..14405
                     /locus_tag="VBE34_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO7264200.1"
                     /translation="MKQDYISYIRSKVGHETIFLTYSGGILTDGKGRVLLQLRADKNS
                     WGIIGGCMELGESSVDTLKREFFEETGLRVEPIRLLNVYTNFQDSYPNGDKAQTVGFI
                     YEVSCPKPVNIEGFHNEETLQLDYFSKEDVKNITIVNEQHQLILDEYFSQTFQMGR"
     gene            14727..>15521
                     /locus_tag="VBE34_001493"
     CDS             14727..>15521
                     /locus_tag="VBE34_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7264201.1"
                     /translation="MKITLHGVAETLLITLYIRAKDAMAKHPILNDQKSLAIVEQIEY
                     DLDKFDNSEASFYATLARIRVMDREIKKFIRENPNSQILSIGCGLDTRFERVDNGQIR
                     WYNLDLPEVMEIRKLFFEEHERVTNIAKSALDETWTREVNPQNAPFLIVSEGVLMFLK
                     EDDVETFLHILTNSFSQFMAQFDLCHKEMINKGKQHDTVKYMDTEFQFGITDGHEIVD
                     LDPKLKQINLINFTDEMSKFELGTLRSLLPTIRKFNNCLGVYEYKAS"
BASE COUNT         5161 a         2321 c         3084 g         4955 t
ORIGIN      
        1 aatttaaaat ataatgaaat taatggctta aaactattgc ttttatttat gattatagta
       61 taatggtaag gtatgttata aacatgcctg tgggaggtaa aaatttctaa ttaccgccaa
      121 aaccacaaca ggaggatttt taaaaatggc taaaaaagtt gaaaaacttg taaaacttca
      181 aattcctgct ggtaaagcaa ctccagctcc accagttgga ccagcacttg gtcaagcagg
      241 aatcaacatc atgggattca ctaaagagtt taacgctcgt acagctgatc aagctggtat
      301 gattattcca gttgttatct cagtatatga agacaaatca tttgatttca tcactaaaac
      361 accaccagct gctgttcttt tgaaaaaagc tgcaggtgtt gaaaaaggat caggtgaacc
      421 taacaaaact aaagttgcaa caattactcg tgcgcaagta caagaaattg ctgaaactaa
      481 aatgccagat ttaaacgctg caaaccttga gtctgcaatg cgtatgatcg aaggtactgc
      541 tcgttctatg ggattcactg ttactgacta atcagaaaca cccacataac ccgcaagact
      601 tcatcgtagt ttgatgagtg acgtgggaga ttttaaatca aatcgattga ccacattaca
      661 aggagaattt taaaatggct aaaaaaagta aaaacttacg tgctgctctt gagaaaattg
      721 acagcactaa agcatacagc gtagaagaag ctgtagctct tgcaaaagaa actaactttg
      781 ctaaatttga tgcaactgtt gaagtttctt ataaccttaa cattgacgtt aaaaaagctg
      841 accaacaaat ccgtggtgct atggttttac cagcaggtac tggtaaaact tcacgtgttc
      901 ttgtttttgc tcgtggtgct aaagctgaag aagctaaagc agcaggtgca gactttgtcg
      961 gtgaagatga cttagttgct aaaatccaag gtggatggct tgactttgat gttgttatcg
     1021 caacaccaga tatgatggca cttgttggac gtcttggacg tgtccttgga cctcgtaact
     1081 tgatgccaaa ccctaaaact ggtacagtaa ctatggacgt tgctaaagca gttgaagagt
     1141 ctaaaggtgg taaaattact taccgtgctg ataaagcagg taacgtccaa gcccttattg
     1201 gtaaagtttc atttgatgat gctaaattag ttgataactt caaagcattt aatgatgtta
     1261 ttgttaaagc taaaccagct actgcaaaag gaacttacat cacaaacctt tcaattacaa
     1321 caacacaagg tgttggtatc aaagttgatc ctaactcact ttaatcaata aaaaactagg
     1381 aattccttcc tagttttatt tattctatat acttaactag ttaagtatat attttaataa
     1441 tcaaaggata ttatatgtgt ttcttttgaa aaatagcttt ttatctgtat taaccttctt
     1501 aaagactaat gaatgtgcga tgtgttttaa gtaacagatg agaataagga aaaatgaaat
     1561 tggcgatatg gtatgtgact attatgatta ttctttagca ataatctatt aaaatgagtt
     1621 cgcagggaat aagtaaattt tgtatcgttg tagatttttt attttaagat tcttgacaag
     1681 acattactaa aatgatttaa ttaagtttgc ttaaaaaaat aattaactaa acttaaaaga
     1741 aagttggtaa aaatgagacg taacatatta ttgagcatta cttgtttatt gatggtgact
     1801 ctgactgcat gccattcaca ggatagcaaa agtcataaac taaacagtga taaactcacg
     1861 cttgcttggg gagaagactt tggtgatgtt aatccgcatc gctataaccc tgatcagttt
     1921 gttatccagg atatggttta tgaggggttg gtacgatatg gtgacaatgg gaaaattgaa
     1981 ccagctttag cgaaaagctg gagcattagt caagatggaa aaacctacac ttttaaattg
     2041 agaaatgcta aatattctga tggcagtaac tttaatgctg cgaatgtcaa acgtaatttt
     2101 gatagtattt tttcgaagtc taatagaggt aatcataatt ggtttaattt aacgaatcaa
     2161 ttggaaaatt atcgtgcctt gaatcaaagt acttttgaaa ttaaactgaa acaagcttat
     2221 agtgctactt tatatgatct ctctatgatt cgaccaattc gctttttatc tgatagcgct
     2281 ttccctaaag gtgatgatac aactaagaag aatgtaaaaa aaaccaattg gaacgggtca
     2341 atgggttgtg aaaagtaaaa agcaaaatga atatattacc ttcaaacgta atgagaatta
     2401 ctggggaaag aaaccaaaat taaaagaagt aaccgtaaaa gttatacccg atgctcagac
     2461 gcgtgcttta gcattcgaat caggtgacgt tgatttgatc tatgggaatg gcattattgg
     2521 tttagacact tttgcccaat atacgaagga caagaaatat gtcacagcta tttctcaacc
     2581 tatgtcaaca agacttttgt tgctaaatgc taaggaaagt attttccaag ataaaaaagt
     2641 tcgtcaggca atgaatcatg ctattgacaa ggtttctatt gctaagaaca cctttagagg
     2701 aacagaaaag ccagcagaca ctattttttc aaaatcaaca tctcactcag atgctaagtt
     2761 aaatccttat agctataatg ttgataaagc aaatcagcta ttggatcaag ctggttggaa
     2821 gatgggaaaa gataaggttc gtgaaaaaga tggtaaagct ctaaccttac gtcttcctta
     2881 tatcgccact aaggcaactg ataaagacct ggtaacttat ttccaaggag aatggcgtaa
     2941 aatcggtatt aatgtttctc ttattgctat ggaagaagat gattattggg ctaatgctaa
     3001 aaagggtaat tttgatatga tgttaaccta ttcttgggga gcaccatggg accctcatgc
     3061 ttggatgtcg gcattaactg ccaaggcaga tcatggacat ccagaaaata tagctttaga
     3121 aaatctagca actaaaactg aaatggatag acttattaag tcagctctag tagatcctaa
     3181 ggaagaaaat gttgatagag actataagaa ggttcttgaa ttattgcatg atgaggctgt
     3241 atatatccca ttaacttatc aatcggtgat ttctgtttat aggaaaggtg attttaaaac
     3301 catgcgtttt gcgccagaag aaaattcatt cccattacgc tatatagaaa aaaacaatgt
     3361 atctaagtag ttcgatcatc aaaaaaatat tgtcagcttt tttggcattg tttttcatat
     3421 cacttctaac atttattctt atcaaactat caacagttaa tccagcagaa aattaccttc
     3481 gtttatcaaa aataagtgtt agtccagaag cgctaaaaga agcagagcac tatttaggtt
     3541 tagataagcc attatggaag cagtactggc tgtggttcca aaaagcatta acaggagatt
     3601 ttggatattc ttatgtgctc aggttgccag tattagattt ggttttacaa cggtttttgg
     3661 ctactttatt tttaggcacg agtgccttct tgctaattgt taccatttct accccgttag
     3721 gtgtgtgggc tggcttacat gaatctgctc ggagtgatca cttgattcga tttttgagtt
     3781 tttctagtgt gtctatgcca aatttttggg ttgcttactt gttgatgctg ctattttcgg
     3841 caaaattaaa tcttctgcct gtttcaggag gtaacgattt acaaagttta attttgccaa
     3901 gtattacctt aagtttttct accgtaggtc aatacatagc ccttattcga aaagctatta
     3961 gtcaagaaaa tagaagttta aatgtggaaa atgcccgatt aagaggagta aaagaacgtt
     4021 atattgtcac acatcacctc cttagaaatg ccttacctgc gataatgaca gcacttagct
     4081 tgacttgggt ttacttatta acaggatcaa ttattgttga agaaattttt tcatggaatg
     4141 gaataggacg tttatttgtg accagtctaa gaacttcaga tcttccagta atacaagctt
     4201 gtatgctaat ttttggaacc ttatttttag ctaataattt tatgacacag tgttttatga
     4261 attgggttga tcctcggtta cgaaagtcaa gggagaaata atatcaaaaa gaatttattt
     4321 ttcgagtagc ttactggtat tgttagtgat tagtgccatt tttgccccta ttctatctag
     4381 ctttgatcca caatacgtag atttatcaca aaaattattg gctcccaata atgttcattt
     4441 gttagggact gaccaattgg gtagagatgt attatctcgc ttgctttatg gtgctagata
     4501 ttcactgttt ttagctatta ttattagctt attggaatta acgattggta tgtttgtggg
     4561 tcttattgtt ggttggtatc aaggaaagtt agaaaacctc tttttatgga tagctaatat
     4621 tattttagct tttccgagct ttttgctctc tcttgcaacc gtggggatct taggtcatgg
     4681 tctagggaat ttaatttttg caattgtctt tgtggaatgg gtttactatg ctaaattaat
     4741 gaccaattta gttaagagtg caaaaaaaga gccttatgtg ataaatgcgc aaattatggg
     4801 actttcagtc tggcatattt taagaaaaca tatttttcct tttgtgtatc agccaatcct
     4861 cgttatggtc cttatgaata taggaaatat tattttaatg atttctggct tttctttctt
     4921 agggattggt gtgcaaccta atgtcacaga atggggaatg atgttgcacg atgctagagg
     4981 atattttagg acagctactt ggatgatgtt atctcctgga attgcgattt ttttaacagt
     5041 attttccttt aatactttag gcgatgccat agataaaaaa gattggaaac gacaatggaa
     5101 cagttagaaa taagaaaatt gtcattacag ataggagagg tccctgtact aagagatttt
     5161 agttgtaaaa tagatatggg agaatcgcta actattattg gtgagagtgg ttcagggaaa
     5221 accctcttgg caaaattatt ggttggtcac attccacaag gtatgacagt tagaggaaac
     5281 atatttttta agggagttga tttaggtaaa ctaactgtaa agcagtggca gaaattaagg
     5341 ggacgagata ttgcctattt agtgcaaaat cccatgtcta tgtttaatcc ttttcaaaaa
     5401 attgaagcgc atattttgga aacaatcctt agtcatgaga aatgttcaaa gagagtagcc
     5461 ttatctaaag cgttagaatg gatgaaacgt ttaaatttag atgatgcgat atccctgtta
     5521 aaaaaatacc cttttgagct tagtggagga atgctacaaa gaattatgtt agccactata
     5581 ttatctcttg atcctcaggt aatcatatta gatgagccga cctcagcggt tgattgtcat
     5641 aattgttcta ctatatcagc tattttgcag gaattgcaaa ataatggaaa aaccttgatt
     5701 actgtaacgc atgattatca gttagctaga gacctggggg gacaattatt agttataagt
     5761 gagggagaag ttgttgaaca gggacaaacc caagctattt taagcaatcc tcaacataac
     5821 tatacgaaag cactgacagt gcaaatggag tatgaaggag atattcttaa tgttggtttg
     5881 taatcatgtc ggaaaaacat ttggacgaca agaggtttta aaggactgtc attttcatct
     5941 taaaagagga gaaataattg gtataatggg taaaagtggc agtggtaaaa gtagccttgc
     6001 ccgactcatt ataggactag atagtcctac ttgtggttcg atatattttc aaggaaaaat
     6061 ttacacacct aaagatggta aggcgcagat tatccttgtt tttcaagatg cgcttagttc
     6121 ggtcaatcca tattttagta ttgaggaaat tttgaatgaa gctttttatg gaaaaaaaaa
     6181 caacttttga attatgtcaa atattagaag cggtgggttt agatggaact tatctaaaat
     6241 ataaagctag acaacttagt ggtggtcagc tacagagagt ttgtatcgct agagctttac
     6301 tcttaaagcc taaaattatt atttttgatg aatccttaag tggattagac ccagtaactc
     6361 aaattaaaat gctacgttta ttacaaaaaa taaaacgccg ttatgaatta agttttataa
     6421 tgatttctca tgaccctaaa atttgtcaag ctatctgtaa ccgggtcttt ctgataaaaa
     6481 atggttattt agtagaagat aatgaattct tgaagagagc gtgttctact aactgtttga
     6541 ccaatctctg aattattatg tttttcttat tttaggagtt gataaagaaa tcaacttact
     6601 agtgtcattg ctctattaaa attttgcctt ttatggtaaa ataataaaga caaaataagg
     6661 agaacatagt agtggaacct aaatatcaac gtatattaat taagttatct ggtgaagcat
     6721 tggcgggaga taagggtgtc ggtattgaca ttcctaccgt tcaatctatt gcaaaagaaa
     6781 ttgccgaagt acacaattca ggtgttcaaa tagcacttgt cattggtgga ggtaatcttt
     6841 ggcgtggaga acctgcagca gaagcaggga tggatcgtgt tcaagctgat tatacaggga
     6901 tgttagggac agtaatgaat gctcttgtaa tggctgatag ccttcaacaa tatggggtag
     6961 atactagagt tcaaacagct attccaatgc aaactgttgc tgagccttac gtacgtggtc
     7021 gtgctttacg tcatctagag aagaatcgta ttgttgtctt tggagcgggt attggttcgc
     7081 cttatttctc aactgataca acagcagcgc ttcgtgctgc tgagattgag gcagaagcaa
     7141 ttttaatggc taaaaatgga gtagatggtg tttataatgc tgatccaaag aaagatgcca
     7201 atgctgtcaa atttgatgaa ttaactcacg ttgaagtaat aaaacgaggg ttaaaaatca
     7261 tggatgcaac cgcatcaact atttcaatgg ataatgatat tgaccttgtt gttttcaata
     7321 tgaatgagac tggtaatatt aagcgtgttg ttcttggaga acaaatcgga acaactgttt
     7381 caaacaaagc atctgaatag gagtgtataa aaaatatgac taaggaaatc gttacaaaag
     7441 ctcaagagcg ttttgagcaa tcacaccaaa gtttatcaag agaatttgca gggattcgtg
     7501 caggacgtgc taatgctagc ttgcttgatc gtattcaagt tgagtattat ggagcgccaa
     7561 ccccacttaa ccaattagct tctattactg tacctgaagc tcgtgttctt ttaatttcac
     7621 catttgataa atcatcaatc aaagatattg agcgtgctat taacgaatca gatttaggta
     7681 ttaatcctgc aaatgatggt tcagttattc gtcttgttat tccagctttg acagaagaaa
     7741 cacgtcgtga tttagctaaa gaagtgaaaa aagtcggaga aaatgctaag attgctatcc
     7801 gtaatattcg ccgtgatgct atggatgaag ctaagaagca agaaaagaat aaagaaatta
     7861 cagaagatga cttgaaatct cttgaaaaag atattcaaaa ggctactgat gatgctgtaa
     7921 aacatattga tgagatgact gctaataaag aaaaagaatt gttggaagtt taatagtaac
     7981 gtttgctatc tttattatga ttggcaagta ataaaatgat tatggaggta actgatagaa
     8041 tcaaaaccag atggatttgg tttgtcggtt acctttttaa aaggagaaaa atgaatacat
     8101 tattagcgac agtcataaca ggcttagtaa ctgatgaaaa caaagatttt tactttatcc
     8161 aaaaagatgg ctttaccttt gcattatcaa agtcagaagg agagcatcac attggtgaaa
     8221 tggttaaagg attcgcctat acagatatgc aacaaaaagc acgtcttaca actaaagaaa
     8281 catttgcgac tcgagatcat tatggttggg ggacagttac tgaggtacgt aaagacttgg
     8341 gagtatttct tgatactggt cttccagata agcaagtggt tgtttctttg gatgttcttc
     8401 ctgaattgaa agaactttgg cctaaaaaag gtgatcgcct ctacgtttgc ttagatgttg
     8461 ataaaaaaga tcgtctctgg gctctccctg cagatcctga agtgtttcaa cgaatggcaa
     8521 cccctgcata taacaatatg caaaatcaaa attggccagc aatcgtttat cgcttaaaat
     8581 tgtcaggaac ttttgtttat ctaccagaaa ataatatgct cggctttatt caccctagtg
     8641 aacgttacag tgaacctcgt ttagggcaag tgctggatgc acgagttatt ggcttcagag
     8701 aagttgatag aaccttaaat ttatcattaa agcctcgttc ttttgaaatg ttagaaaatg
     8761 acgcacaaat gattttaacg tatttagaaa gtaacggagg attcatgact ttaaatgata
     8821 aatcttctcc agaggaaatt aaagctactt ttggaatttc caaaggtcag tttaaaaaag
     8881 cacttggagg actcatgaaa gctaaaaaga tcaaacaaga tcagctaggt acagaattat
     8941 tataaacttt tgaactttta aagttttctc acgaggtcgt tttagttata gctagaatta
     9001 atggctaatt atagaaaaaa gctgagagga aattttggct ttttttgtta taatgtatct
     9061 attaaaaaga atggagaacg atatggaacg tgcgattttt gctggaggtt gtttttggtg
     9121 tatggtacaa ccatttgaag aattagatgg tattgaatct gttttatctg gatacactgg
     9181 aggacatgtt gagaatccaa cttataaaga agtttgtagt aaaactactg gacatacaga
     9241 agctgttgaa attattttta atcctgaaaa aatctcttat gcagatttag ttgagttata
     9301 ttgggcacaa acagatccaa cagacgcttt tggtcagttt gaagatcgtg gggataatta
     9361 tcgtcctgtc atcttttatg agaatgaaga gcaacgtcag attgctcaaa aatcaaaaga
     9421 taagctacaa gcttcgggta gatttgatag accaattgta accagcattg agcctgctga
     9481 tactttttac ccagcagaag actatcatca agcattctat cggactaacc ctgctcgtta
     9541 cgccttgagt agtgccagaa gacatgcatt tctagaggag aactggcatt gagaaaatca
     9601 ttttacagtt ggttgatgac acaaagaaat ccaaaatcaa atgaaccagt agcaatccta
     9661 gcagattacg cttttgatga aaccactttt ccaaaacata gttcggattt tgaaacagtt
     9721 agtcgctatt tagaagatga ggccagtttt tcctttaatt taacagattt tgatgatatt
     9781 tgggaagatt atctcaatca ttaacaataa tatagagatt gtattttcaa tctctttttt
     9841 aggttataga aattggacac attgtctaag actgtctgaa aagcaggttt tttaatggca
     9901 atcgttttca caaaggagta gaatatagtc atctaaaata tagccctagg gaggttgttt
     9961 atgagatata caaatggaaa ttttgaagcc tttgcaagac ctcgaaaacc tgaaggtgtg
    10021 gataaaaaat ccgcttatat tgttggttct ggtttagcag gattagctgc cgctgtcttt
    10081 ttaatacgtg acggtcaaat ggatggtcaa cgtattcata tttttgaaga actacctctt
    10141 tctggaggat cacttgacgg tgtcaaacga cctgatatcg gttttgtaac gcgtggtggt
    10201 cgtgaaatgg aaaatcactt cgaatgtatg tgggatatgt accgttccat cccctctctc
    10261 gaagttccag atgcttctta tctagatgaa ttttattggc ttgacaagga tgatcccaat
    10321 tcatctaact gtcgcctcat tcataaacag gggaatcgct tagaatctga tggtgatttt
    10381 acactcggaa cacattccaa agagttagtt aagctagtca tggagactga agagtcttta
    10441 ggtgctaaga cgattgaaga agttttttca aaagaatttt ttgaaagtaa tttttggact
    10501 tattgggcta ctatgtttgc ctttgagaaa tggcattcag cgattgaaat gcgtcgatat
    10561 gctatgcgct ttatccatca tattggtggt ctgcctgatt tcacttcatt aaaatttaat
    10621 aaatataatc aatatgattc tatggtgaaa ccaatcatca gttatttaga gtctcataat
    10681 gtagatgttc aatttgatag caaggtaact aatatctccg tagactttaa gaacggtcag
    10741 aagcttgcaa aagccattca tctcacagtt ggtggtgaag caaaaacaat tgatcttact
    10801 ccaaatgatt ttgtttttgt aacgaatggt tctatcactg aaagtacgaa ctatggtagt
    10861 catgatactg tagctaaacc taataccgat ttgggaggtt cttggaatct ttgggaaaac
    10921 ttagctgctc aatctgatga atttggacat cctaaagtct tctacaaaga tattccaaaa
    10981 gagtcatggt ttgtttctgc tactgcaaca ataaaagatc cagctattga accttatata
    11041 gagcgtttaa cacatcgtga cctccatgat ggtaaagtaa ataccggagg cattgttaca
    11101 gttacagatt ctaattggat gatgagtttt gctatccatc gccaaccgca tttcaaagag
    11161 caaaaggaaa atgaaacaat tgtttggatt tatggactct attcaaatgt ggaaggtaat
    11221 tatattaaaa aaccaattga agaatgtact ggtcgagaaa tcacagaaga gtggttatat
    11281 cacttaggtg ttcctgaaat gaagattcat gatttatctg ataaacaata tgtaagtaca
    11341 gttcctgttt atatgcctta cattactagt tatttcatgc ctcgagttaa aggtgaccga
    11401 ccagatgtta tcccacaagg atctgttaac ctagccttca ttggtaattt tgcagaatct
    11461 ccatccagag atacagtatt tacaacggaa tattctatta gaactgctat ggaagctgtt
    11521 tatactttct taaatattga acgtggtgtt ccagaagtct ttaattcagc atttgatatt
    11581 cgtgtcttgt tgcaatcttt atactatctc aatgataaga aatctgttga agatatggat
    11641 ttaccaatac ctgccttaat gcgtaaagta ggcatgaaaa aaattagagg aacttattta
    11701 gaagaattac ttcgagaagc tcatctttta taatgtaaaa taaaatcgag aaaacttgtt
    11761 tttctcgatt ttttagtttt acaacgacta aaatcaccaa agaaaatgaa attttgttat
    11821 aatgtagtca cttactgtac gaaaaggaag aataatttgc aagaatactc tatcgaaatt
    11881 actttacaac atccagatga tatgatgtca ctctttggca gcaatgagcg ccatttaaaa
    11941 ttaatcgaag aaaatttaga tgttatcatt catgctagaa ctgaacgtgt tcaggtatta
    12001 ggtgactctg aagaagcagt agaaaccgct cggttaacca ttgaagcatt attagtgctt
    12061 gttaaccgcg ggatgactgt gaatacttca gatgttgtaa cagccttatc catggcacaa
    12121 aatggttcaa ttgataaatt cgttgccctt tatgaggaag aaattattaa ggatagctat
    12181 ggtaagccta tccgtgttaa gactttaggg caaaaaatat acgttgatag cgttaaaaat
    12241 catgatgttg tttttggtat tgggcctgct ggaacgggaa aaactttttt agcagtcact
    12301 ctagcagtaa cggcactaaa gcgtggtcaa gttaaacgaa taatcttaac gagaccagcg
    12361 gttgaagctg gtgaaagcct tggtttcttg cctggagatc tcaaagagaa agttgatcct
    12421 tatttgagac cagtttatga tgccctatat caaattttag gtaaagagca aacaagtcgc
    12481 ttaatggagc gtgaaatcat cgaaattgct ccattggctt acatgcgtgg tcgtacttta
    12541 gatgatgcat ttgtcatatt agatgaagcg caaaacacca ctataatgca aatgaaaatg
    12601 ttcctaacac gtcttggctt caattctaaa atgattgtaa atggcgatgt tagccaaatt
    12661 gacttaccaa agaatgttaa atctggtttg attgatgctg ttgagaaatt acgaaatatc
    12721 aaaaagattg actttattca tttatcagca aaggatgttg taagacaccc tgtcgttgct
    12781 gaaataataa atgcttattc agattcagaa agtagtcaca agctacaatc tgataaggac
    12841 tgatgtttgc cactaggaga cgttgttaga gagtgtgtca atatgtaaaa aacgcctaat
    12901 gctttaggcg tttttatgat ggtgtacttc aacttaacct ataaagtgtt gaaaattttc
    12961 tttaagcatg acgtcgagat gcattattat atcgtttaat gtttctttaa cagtgtgttc
    13021 gttggagttg tgatgacata gtttaatgat attttcttcg aaatgtttag gaagcaatgt
    13081 agcattcttc ttagcatagg aaatcattct tttttcgcca ggatgtgtca acttattaag
    13141 tgcaaaaata atatcaaagt aggaatctaa gaaagcagta gttctatggt gagtactaac
    13201 aaagtcttgg cgtttaaggg cttttataat ttgtttatcg taagagggaa gcttgccagt
    13261 taataagtta cgattttgaa tgataatttg tttttgtaac tgactgggat aagaaacttc
    13321 gaatctctct ttgagtgatt gtaattgatt ttcaggatca tagagtattt ggcaattgat
    13381 gaggttatgc caaaaacaag tagtgtatcc aattcgagaa ttgtggtgtt caacgacatc
    13441 ttctaagtct gataaaaagt tatctatgtt acgatagaga atatcaatat cagttccgtc
    13501 gtttaaagta ccattatctt caagctccca gtaatgattg ttaagttcaa tgtaatgaca
    13561 gtgcttgtta aggatacgtt tacgactagt gatatcagga gttgctgcac aataaacata
    13621 caaatcataa tcggaagatt ggtcaaaact gtcaccagag cgtgagcctc cgagtgctat
    13681 agcaacaaca tttggaagtt tagagaaatc ttgccagagt tttgtgaaat ggtccataaa
    13741 tgctctccta aaaatgtaaa atattgatgt gtctccattc tacagggcta tctcttttta
    13801 gtcaataatt aatagtaagt taataaaaga ggtaacggtt ctaatattca agaagtgtat
    13861 atgagtgatt tgaaaaggag atattttatg ctacaatacg tacatattag tttaaacaag
    13921 gtgatagaat gaaacaagat tatatttcct acattaggtc aaaagttgga catgagacta
    13981 tctttttaac atattctggt ggtattttga cagatggtaa gggacgtgtc ttactccaat
    14041 taagagctga taagaattct tggggaatta ttggtggatg tatggagtta ggtgagtcat
    14101 cagtagatac attgaaaaga gaatttttcg aagaaactgg attgagagtg gaaccaatca
    14161 gactgttaaa tgtttatact aactttcaag actcttatcc caatggcgat aaagcacaaa
    14221 ccgtcggttt tatttatgaa gtaagctgtc caaaaccagt taatatagaa ggttttcata
    14281 atgaggaaac attgcaattg gactattttt ctaaagaaga cgtcaaaaat attacgatcg
    14341 taaatgaaca acaccaatta attttagatg aatatttttc acaaacattc caaatggggc
    14401 gttaaaagga tgggaaatac taggtgtgac tttaaagaac ttctaacttt tattgatatg
    14461 ttgttaggca ttcttgtaga aacaaatgtt aagaaaataa ggagttctca tttgtatatt
    14521 ttataattat taaaaagaaa aacagtagct tgcaataatt tagtgactgt ttttttagaa
    14581 agcataagaa gtacagaaaa agaaaattat ggaatattgg atggtgggga tatgctttac
    14641 ttacggtatt ggaatgatat acttaaagaa atccagtttt agaaatccaa atttctaagt
    14701 aaaatagcat aaaggagagt agatgaatga agataacatt acacggcgtt gctgaaacgt
    14761 tattaatcac cttatatatt agggctaaag atgcgatggc taaacatccg atacttaatg
    14821 atcaaaaatc cttagcaatt gttgaacaga tagaatatga tttggataaa ttcgataatt
    14881 cagaagcttc tttttatgca acattagcta gaattcgcgt tatggataga gaaatcaaaa
    14941 aatttattag agaaaatcca aatagtcaaa tcctttcaat tggttgtgga cttgatacaa
    15001 ggtttgaaag agtcgataat ggacaaatta ggtggtataa ccttgatttg ccagaggtta
    15061 tggagataag aaaattattt tttgaagagc atgaaagagt tactaatata gcaaaatcag
    15121 ccctagatga aacttggaca cgggaggtaa atccccaaaa tgcccctttt ctaatcgtgt
    15181 cagaaggtgt tttaatgttt ctaaaagaag atgacgtaga gacttttctt catatcctga
    15241 caaattcatt tagccaattt atggcacaat ttgatttgtg tcataaggaa atgattaata
    15301 aaggaaagca acatgataca gtaaagtata tggatacaga atttcagttt ggtatcacag
    15361 atggtcatga gattgtggat ttagacccta aattaaagca aataaatctg attaacttta
    15421 cagatgagat gagcaaattt gagttaggca cacttcgctc tttacttcca acaattcgta
    15481 aatttaataa ttgtttaggt gtgtacgaat ataaagcatc t
//
LOCUS       DATRUK010000052        15248 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.052, whole genome shotgun sequence.
ACCESSION   DATRUK010000052 DATRUK010000000
VERSION     DATRUK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15248
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.052"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(80..1531)
                     /locus_tag="VBE34_001494"
     CDS             complement(80..1531)
                     /locus_tag="VBE34_001494"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEO7264202.1"
                     /translation="MITIDKILEILKNDHNFREILFHEHYYYNWTQNVTFNALSYDSR
                     QISSDTLFFAKGATFKKEYLDSAITAGLSFYVSEIDYGADIPVILVNDIKKAMSLISM
                     SFYNNPQNKLKLLAFTGTKGKTTAAYFAYHMLKVNHRPAMLSTMNTTLDGKSFFKSHL
                     TTPESLDLFRMMATAVENQMTHLVMEVSSQAYLTKRVYGLTFDVGVFLNISPDHIGPI
                     EHPTFEDYFFHKRLLMENSNAVVVNSQMDHFNIVKEQVEYIPHDFYGDYSENVITESQ
                     AFSFHVKGKLENTYNINLIGKFNQENALAAGLACLRLGVSIEDIKNGIAQTTVPGRME
                     VLTQTNGAKIFVDYAHNGDSLKKLLAVVEEHQKGDIILVLGAPGNKGQSRRKDFGDVI
                     NQHPNLQVILTADDPNFEDPLVISQEIASHINRPVTIIIDREEAIANASTLTNCKLDA
                     IIIAGKGADAYQIIKGNRDNYSGDLEVAKKYLK"
     gene            1655..3289
                     /locus_tag="VBE34_001495"
     CDS             1655..3289
                     /locus_tag="VBE34_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO7264203.1"
                     /translation="MSQKTTKVSQQEQMVKGTAWLTAGNFISRLLGAIYIIPWYVWMG
                     KHAAEANALFGMGYEIYALFLLISTVGIPVAVAKQVSKYNTLGKEEMSIYLVRKILQF
                     MLILGGVFALIMYIGSPLFASLSKGGQELVPILRSLTLAVLVFPSMSVLRGFFQGFNN
                     LKPYAISQVAEQIIRVIWMLLTAFYIMRLGSGDYIAAVTQSTFAAFVGMFASIAVLLY
                     FLWRYNMLSALISKTPEHIKLDTKEILIETVKEAIPFIITGAAIQIFKLIDQFSFGNT
                     MALFTNYSSEELRVMFAYFSSNPGKVTMILIAVATAIAGVGIPLLTENFVKNDKKAAA
                     RLVVNNLQMLLMFLLPAVAGSVILAKPLYTVFYGLPQGQALGLFVISLIQTIILSIYT
                     VLAPMLQALFENRKAIIYFLYGLVAKVILQVPSIFLFHAYGPLFSTTVALCIPVILMY
                     LKIHEITGFKRQAIRRTSALVLILTLLMSFIISMIIWLMNLVIVPDSRLVSLVYIIVI
                     GAIGLGVYGFMALATHLLDKMIGSRAQDLRRKLHLN"
     gene            3424..4644
                     /gene="pepT"
                     /locus_tag="VBE34_001496"
     CDS             3424..4644
                     /gene="pepT"
                     /locus_tag="VBE34_001496"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEO7264204.1"
                     /translation="MSYEKLLERFLTYVKINTRSNPNSTQTPTTQSQVDFALTVLKPE
                     MEAIGLKDVHYLPSNGYLVGTLPATSDRLRHKIGFISHMDTADFNAENITPQIVDYKG
                     GDIELGDSGYILSPKDFPNLNNYHGQTLITTDGKTLLGADDKSGIAEIMTAMEYLASH
                     PEIEHCEIRVGFGPDEEIGIGADKFDVKDFDVDFAYTVDGGPLGELQYETFSAAGLEL
                     TFEGRNVHPGTAKNQMINALQLAIDFHSQLPENERPEQTDGYQGFYHLYDLSGTVDQA
                     KSSYIIRDFEEVNFLKRKQLAQAIADNMNEALQSERVKVKLYDQYYNMKKVIEKDMTP
                     INIAKEVMEELDIKPIIEPIRGGTDGSKISFMGIPTPNLFAGGENMHGRFEFVSLQTM
                     EKAVDVILGIVAKD"
     gene            4673..5164
                     /locus_tag="VBE34_001497"
     CDS             4673..5164
                     /locus_tag="VBE34_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEO7264205.1"
                     /translation="MIKLFGKIRYHWQPELSWAIIYWSIAIAPIFIGLSLLYERTEIP
                     SQVFVLFAIFIVLVGIGFHRYFVIEEDGYLRIVSFNFLRRSKFPIEDIAKIEVTKSSV
                     TIKFNNNHERIFYMRKWPKKYFLDALAIEPKFKGEVELLDNLIKMDYFECYRYDKKAL
                     TKS"
     gene            complement(5151..5348)
                     /locus_tag="VBE34_001498"
     CDS             complement(5151..5348)
                     /locus_tag="VBE34_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEO7264206.1"
                     /translation="MKVALIPEKCIACGLCQTYSNIFDYQDDGIVKFSDTDNLEKEIP
                     SSDQDTVLAVKSCPTKALTIL"
     gene            5394..5918
                     /locus_tag="VBE34_001499"
     CDS             5394..5918
                     /locus_tag="VBE34_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO7264207.1"
                     /translation="MAKKPWEKKVVENNSHRKDKITRTSRGVVSSTPWITAFLSAFFV
                     IVVAILFIVFYTSNRGEDRAKETSGFYGASSQKVNSSKTKKASTSKKTTSSSKTKTSD
                     KSTTSSSTVASSSETSTSSSVNGGTIVVQPGEGAASIAARAGISVARLQELNPGHMTS
                     GYWYANPGDPVVVN"
     gene            5929..6612
                     /locus_tag="VBE34_001500"
     CDS             5929..6612
                     /locus_tag="VBE34_001500"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEO7264208.1"
                     /translation="MNSINIAIDGPASSGKSTVAKIIAKNLNYTYLDTGAMYRCATYL
                     ALQHGYEAQDVSKILGLLAERPISFGKAEDGSQTVFLGTEEVTLAIRQNDVTNNVSWV
                     SAIPEIREELVNQQRRIAKDGAIIMDGRDIGTVVLPDAELKIFLVASVDERAERRFKE
                     NQEKGIESDFETLKSEIAARDYKDSHREVSPLKAAEDAIEFDTTGVSIEGVVTFIQEK
                     AEKIIDMKN"
     misc_feature    6633..6758
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            6773..7303
                     /gene="infC"
                     /locus_tag="VBE34_001501"
     CDS             6773..7303
                     /gene="infC"
                     /locus_tag="VBE34_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEO7264209.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQAIADD
                     ANVDLVLIQPQATPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPIPDKK"
     gene            7343..7543
                     /gene="rpmI"
                     /locus_tag="VBE34_001502"
     CDS             7343..7543
                     /gene="rpmI"
                     /locus_tag="VBE34_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEO7264210.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFHAFTSHRFHGKTKKQRRHL
                     RKATMVSSGDFKRIKAMLTRLK"
     gene            7601..7960
                     /gene="rplT"
                     /locus_tag="VBE34_001503"
     CDS             7601..7960
                     /gene="rplT"
                     /locus_tag="VBE34_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEO7264211.1"
                     /translation="MARVKGGVVSRKRRKRVLKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AAAFTALADAAKAKLGK"
     gene            complement(8203..10347)
                     /locus_tag="VBE34_001504"
     CDS             complement(8203..10347)
                     /locus_tag="VBE34_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEO7264212.1"
                     /translation="MKKIKDFASRAINTRLGFILLLVVIYWLKTIWAYHTDFNLGLEN
                     SYQLFLTIINPIPLGLLIIGLALYVKRTKAFYITAFITYAIVNILLIANAIYYREFSD
                     FITVSAVLASSKTSAGLGDSALNLLRIWDLVYVFDFIILIFLFATKKIHLDNRPFNKR
                     ASFSITALSGLLFSINLFLAEIDRPELLSRGFSNTYIVKALGLPSFSIYSGNQTYQAQ
                     KERNGATAQELATAKKYVAEHYAKPNPEYYGIGKGRNVIMIHLESFQQFLIDYKLNVD
                     GKEHVVTPFINSLYHSKETISFSNFFHQVKAGKTSDAETLMENSLFGLSSGSFMVNYG
                     GENTQFAAPHILAQNGGYSSAVFHGNVGTFWNRNNAYKQWGYDYFFDSSYFSKQTKDN
                     SFQYGLNDKYMFADSIKYLEHMQQPFYTKFITVSNHYPYTSLKGESDEEGFPLAKTND
                     ETINGYFATANYLDTALKSFFEYLKAAGVYDNSIIVMYGDHYGISNTRNPSLAELLGK
                     DPETWSEYDNAMLQRVPYMIHIPGYSKGFISNTYGGEVDNLPTLLHILGIDTSKYTQL
                     GQDLLSKDNKQMVAMRTTGQYITPKYTNYSGHLYYTDSGQEITNPDETTKAEIKTIRD
                     ATNKQLSTSDSIQTGDLLRFDENNGLKTVEVEKFNYTHSLKALKAKERKLKDRSTSIY
                     SKHNNKSTVDLFHAPSYLELQDPNKTHKTSKK"
     gene            10482..11639
                     /locus_tag="VBE34_001505"
     CDS             10482..11639
                     /locus_tag="VBE34_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEO7264213.1"
                     /translation="MNKLKVNSVVERKIKSGAQLLEKKDFDTSLVNQLVQLFSQSNQF
                     LGMAYLSPQNKGIGWLLSRQIFDFNHDYFVSLFEKSREKRQKFEKSSQTTAYRLFNQD
                     GDNFGGLTIDFYSDYALFSWYNEFVYTNRQMIVAAFKQVYPNIKGAYEKIRFKGLDFE
                     SAHLYGQEAPESFLILENNIKYSVFLNDGLMTGIFLDQHDVRKALATNLSEGKKVLNM
                     FSYTAAFSVAAAVGGALETTSVDLAKRSRELSKAHFDANQIVTDNHRFIVMDVFEYYK
                     YAKRKHLSYDVIVIDPPSFARNKKQTFSVTKDYYKLIEQALDILAPGGTIIASTNAAN
                     LTVSQFKKQLEKGFGKASHNYISLQQLPEDFTVNDKDQQSNYLKVFTIKVK"
     gene            11639..12316
                     /locus_tag="VBE34_001506"
     CDS             11639..12316
                     /locus_tag="VBE34_001506"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEO7264214.1"
                     /translation="MKIVVPVMPRSLEEAQEIDLSKFDSVDIIEWRADALPKDDIINV
                     APAIFEKFAGHEIIFTLRTTREGGNIVLSDAEYVELIQKINSIYNPDYIDFEYFSHKE
                     VFQEMLEFPNLVLSYHNFQETPENIMEIFSELTALAPRVVKIAVMPKNEQDVLDVMNY
                     TRGFKTINPDQVYATVSMSKIGRISRFAGDVTGSSWTFAYLDSSIAPGQITISEMKRV
                     KALLDAD"
     gene            12410..13477
                     /locus_tag="VBE34_001507"
     CDS             12410..13477
                     /locus_tag="VBE34_001507"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEO7264215.1"
                     /translation="MQVEVDLPNHPYHIKIEEGCFSEAGDWVSHLWQKQMITIITDSN
                     VEILYGESLVNQLKKQGFTVHVFSFAAGEASKTLEVANRIYGFLAKHHMTRSDGIIAL
                     GGGVVGDLAAFVASTYMRGIHFLQIPTSLTAQVDSSIGGKTGVNTSFAKNMVGTFAQP
                     DGVLIDPVTLKTLGNRELVEGMGEVIKYGLIDDIKLWHILEEMDGSIDSILDNALAII
                     YHSCQVKRKHVLADQYDKGLRMHLNFGHTIGHAIEVHAGYGEIMHGEAVAIGMIQLSR
                     VAERKNLMPRGISQDIYNMCLKFGLPVHYAEWDKDVLFNILSHDKKASGQFIKIVILP
                     QLGSATVHQIPLEEMRDYLEK"
     gene            13478..14644
                     /gene="aroC"
                     /locus_tag="VBE34_001508"
     CDS             13478..14644
                     /gene="aroC"
                     /locus_tag="VBE34_001508"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEO7264216.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLKLSAKDINEDLKRRQGGYG
                     RGNRMKIETDQVIISSGVRHGKTLGSPITLTVTNKDHSKWLDIMSVEDIEERLKQKRR
                     IKHPRPGHADLVGGIKYRFDDLRNALERSSARETTMRVAIGAIAKRILKEIGIEIANH
                     IVVFGGKEITVPDKLTVQQIKVLSSQSQVAIVNPSFEQEIKDYIDSVKKAGDTIGGVI
                     ETIVGGVPVGLGSYVHWDRKLDAKIAQAVVSINAFKGVEFGLGFKSGFLKGSQVMDSI
                     SWTKDQGYIRQSNNLGGFEGGMTNGEPIVVRGVMKPIPTLYKPLMSVDIDTHEPYRAT
                     VERSDPTALPAAGVVMEAVVATVLVTEVLEKFSSDNMYELKEAVKLYRNYVNHF"
     gene            14728..15063
                     /locus_tag="VBE34_001509"
     CDS             14728..15063
                     /locus_tag="VBE34_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEO7264217.1"
                     /translation="MANVYDLANELERAVRALPEYQAVLTAKAAIENDADAQVLWQDF
                     LATQSKVQEMMQSGQMPSQEEQDEMSKLGEKIESNDLLKVYFDQQQRLSVYMSDIEKI
                     VFAPMQDLM"
BASE COUNT         4946 a         2394 c         2973 g         4935 t
ORIGIN      
        1 agaaaaatgt gaacgaaatg ataattattt agaatgattc aaaatagtat caataatcat
       61 tattgtttag ctttttttat catttaaggt attttttcgc aacttctaaa tctccagagt
      121 aattgtcacg atttcctttg ataatttgat aagcatctgc ccctttacca gcgataataa
      181 ttgcatctag tttgcaatta gtaagagtac tggcattagc aattgcttct tctcgatcaa
      241 tgatgatagt aaccggacga ttaatatggc ttgcgatttc ttgtgagata actaagggat
      301 cttcaaaatt aggatcatct gcagtgagta tcacttgtag attaggatgt tgattaatga
      361 catctccgaa gtctttacga cggctttgac ctttattacc aggagctccg agcactaata
      421 taatatcccc tttttggtgt tcctcaacaa cagctaataa tttcttcaaa ctgtctccgt
      481 tgtgggcata gtcaacgaaa atctttgcgc cgttggtttg tgttaggact tccatgcggc
      541 cagggacggt agtttgtgca attccgtttt ttatatcttc aattgaaaca ccaagtcgga
      601 gacaagcaag tccagctgcg agagcatttt cttgattgaa ttttccaatc aggttgatat
      661 tgtaggtgtt ctccaacttt cctttgacat ggaaagaaaa ggcctgtgat tctgtgataa
      721 cattttcaga ataatctcca tagaaatcat gaggaatata ttctacctgt tctttgacaa
      781 tattaaaatg atccatctga ctatttacaa cgacagcatt gctattctcc atcaagagcc
      841 gtttgtggaa aaaataatct tcgaatgtag ggtgctcgat aggaccaata tgatcgggac
      901 taatatttaa gaaaacgcca acgtcaaagg ttaaaccata aacacgtttg gttaggtatg
      961 cttggcttga tacttccatg actaagtgtg tcatttgatt ctcaacggct gtagccatca
     1021 tccgaaataa atcaagactc tcgggggttg tgagatgtga tttaaagaaa gatttaccat
     1081 ctaatgttgt gttcatagtt gagagcatag caggacgatg gtttactttt agcatatgat
     1141 aagcaaagta agccgcagtt gtttttcctt ttgttccagt aaaagctaga agtttaagtt
     1201 tgttttgtgg gttattatag aaagacatag atatcaaact catggctttt ttgatatcat
     1261 tgactaatat cactggaata tcggcgccgt aatctatctc agaaacgtaa aaagaaaggc
     1321 ctgctgtgat tgcagaatct agatattctt ttttaaaagt ggcaccctta gcaaaaaata
     1381 aagtatcaga tgaaatttga cgactatcat atgataaggc attaaaagtt acattttgtg
     1441 tccagttata ataataatgc tcatgaaaca aaatctctct gaaattatgg tcatttttta
     1501 aaatttctaa aattttatct attgtaatca tactccttat tttagacttt atgttctcgt
     1561 tttacaagtc taaggtaaaa gtttataata aagtgatatt tctgagattt ttgtctttta
     1621 aatgctaaat aatattgaaa atagaaagtt taccatgtca caaaaaacaa caaaagttag
     1681 tcagcaagaa caaatggtca aagggacggc ttggttaaca gcagggaatt tcattagtcg
     1741 cttgctggga gccatctata ttatcccttg gtatgtttgg atgggaaagc atgctgccga
     1801 agcaaatgct ttatttggga tgggatatga aatctatgct ttatttcttt tgatatcaac
     1861 tgttggaatt ccagtagcag ttgcaaaaca agtatctaag tataacactt taggtaaaga
     1921 agagatgagc atttatctgg ttcggaaaat cttgcagttt atgctgattt taggtggagt
     1981 ttttgcatta attatgtata ttggttcacc tctatttgct tcacttagta aaggagggca
     2041 agaactcgtt ccaatattgc gtagtctaac tctagctgtt ttagtttttc cttcaatgag
     2101 tgttttacgt ggtttttttc aaggttttaa taatttaaaa ccgtatgcca ttagtcaagt
     2161 cgctgagcag attatacgtg tcatatggat gttattaact gctttctata ttatgcgtct
     2221 aggatcagga gattatattg ctgctgtgac acagtcaaca ttcgccgcct ttgtcggtat
     2281 gtttgctagt atagctgtgc tgctttattt cttatggcgt tataatatgc tatctgcctt
     2341 gattagtaaa actccagagc acattaaatt agatactaaa gaaatcctaa ttgagactgt
     2401 taaggaagca atccctttta ttattactgg tgcagcaatt caaattttca aattgattga
     2461 tcagttttca tttgggaata cgatggcgct ctttacgaat tattcaagtg aagaattacg
     2521 ggttatgttt gcttactttt caagcaaccc tggtaaagtt acgatgattc taattgctgt
     2581 tgctaccgca attgctggag ttggtattcc attgttaaca gagaattttg ttaagaatga
     2641 taaaaaagcc gccgctcgtt tggttgttaa taatttacaa atgttactaa tgtttttact
     2701 gccagctgtt gcaggatccg ttattttagc aaaacctttg tatacagtct tttatgggtt
     2761 accacaggga caagctttag ggctttttgt catatctctt attcaaacaa ttattttatc
     2821 tatttatact gttttagcac caatgctaca agcattgttt gaaaatcgta aagctattat
     2881 ttacttccta tatggtctgg tggcaaaagt tattttacaa gttccctcca ttttcctttt
     2941 ccatgcctat ggaccacttt tctcaacgac agtagctctt tgtatcccag taattcttat
     3001 gtatttgaaa atacatgaaa ttacaggatt taaacgccaa gctattcgtc gtacaagtgc
     3061 tttagtcctt attctaacgc tattgatgag ctttattatt tctatgataa tatggttaat
     3121 gaacctggtt attgtacccg atagtagact ggtaagtcta gtttacatta tcgttatcgg
     3181 tgcaataggt ttaggggtat atggctttat ggcactggcc acccatctac ttgataagat
     3241 gattggtagt cgtgctcaag acttacgccg aaagttgcac cttaactaat gaaaaaatag
     3301 gttagacaga attgtttaac ctagtttttt gacgaccaaa attatttgtg aaattttaag
     3361 gtagtcacaa tatagtgagc ttatttttat gttataatta aatggagaaa tatgaggtga
     3421 aagatgtctt acgaaaagct tttagaacga tttttaacat acgtcaaaat aaatacaaga
     3481 agtaatccta atagtacaca aacgccaaca actcaaagtc aagttgactt tgctttaaca
     3541 gttttaaaac cagaaatgga agcaattggt ttaaaagatg ttcattattt accttctaat
     3601 gggtatttgg ttggaacctt acctgctaca agcgaccgct tacgccataa aataggtttt
     3661 atatcccata tggatacagc tgatttcaat gctgaaaata ttactccaca aattgttgac
     3721 tataaaggtg gagatattga acttggagac tcaggttaca ttttaagtcc gaaagatttt
     3781 ccaaatttaa ataattacca tgggcaaaca ttgattacaa cagatggtaa aaccttactg
     3841 ggagcagacg ataagtctgg tatagcagaa atcatgacag ctatggaata tttggcttcg
     3901 catccagaaa ttgagcactg tgaaattaga gttggctttg gaccagatga agaaattggt
     3961 ataggtgcag ataaatttga tgttaaagat tttgatgttg attttgccta tacagtggat
     4021 ggtggaccac taggagaatt acagtatgaa acctttagtg cagctggttt ggagcttaca
     4081 tttgaaggac gaaacgttca ccctggaact gcaaaaaatc aaatgattaa tgctttacag
     4141 cttgctatag attttcatag tcaattacca gaaaatgaac gtcctgaaca aacagatggc
     4201 tatcaaggat tttatcactt atatgattta agtggaacag ttgatcaagc taaaagttca
     4261 tatatcattc gagattttga ggaagttaat ttcttaaaac gtaagcaatt ggctcaagct
     4321 atcgctgata atatgaatga agcattacaa tctgaacgtg taaaggttaa actatacgat
     4381 caatattaca acatgaagaa agttattgaa aaagacatga cacctatcaa cattgctaaa
     4441 gaagtaatgg aagagttaga catcaagccg atcatagaac cgattcgtgg tggtacagat
     4501 ggctctaaaa tttcctttat gggaatccct actcctaatc tttttgcagg tggtgaaaac
     4561 atgcatggac gctttgaatt cgttagtcta caaacaatgg aaaaagcagt tgatgttatt
     4621 ttaggcatcg ttgctaagga ttagtaaaga aatatcagat tggagtggtt aaatgataaa
     4681 attatttggg aagatacgtt atcactggca acctgaactt tcttgggcta ttatttattg
     4741 gtcaatagcc attgcaccaa tttttattgg tttatctctg ctatatgaaa gaactgagat
     4801 tccgagtcag gtatttgttc tatttgccat ctttattgtt ttagtaggca ttggatttca
     4861 tcgctatttt gttattgaag aagatggata tcttaggatt gtatctttta actttttaag
     4921 acgatctaag tttccaattg aagatattgc taaaattgag gtcactaaat caagtgtcac
     4981 tatcaaattt aataataacc atgagcgtat tttttacatg cgaaaatggc ctaaaaaata
     5041 ttttttagat gcactagcta ttgaacctaa atttaaggga gaagtggagc ttctcgataa
     5101 tttgattaaa atggattatt ttgaatgtta tcgctacgat aaaaaagctc ttacaaaatc
     5161 gtaagagctt tggtgggaca tgatttaact gctaaaacag tatcttgatc agaacttgga
     5221 atttcttttt ctaggttatc tgtatcggaa aatttaacga taccatcatc ttgataatca
     5281 aaaatatttg aatatgtttg gcaaagcccg caggcaatac atttttctgg aattagtgca
     5341 actttcatag tattattgta atacttattg atacgattaa caagggggaa gtcatggcga
     5401 aaaaaccatg ggaaaaaaaa gttgtggaaa acaatagtca tagaaaagac aaaattacaa
     5461 gaacgagtag aggtgtggtg tcaagtactc cttggattac cgctttcttg agtgcattct
     5521 ttgttattgt cgttgctatt ttatttatag tattttatac ttcaaataga ggagaagaca
     5581 gagcaaaaga aacttctggt ttttatggtg cttcaagtca aaaagttaat agttctaaaa
     5641 cgaaaaaagc atctacaagt aagaaaacaa cgtcatcatc aaaaactaaa actagtgata
     5701 aatcaacaac atcttctagt acagtagcaa gttcgagtga gacatcgaca tcatcttcag
     5761 taaacggtgg aacaatcgta gttcaaccag gtgagggagc agcctccata gctgctcgtg
     5821 caggtatctc agttgctcgt ttacaagaac ttaatcctgg tcatatgacc tcagggtatt
     5881 ggtatgctaa tccaggcgat cctgtggttg taaattaagg ggaggattat gaactcaatt
     5941 aatattgcta ttgatggacc agcatcaagt gggaaaagta ctgtcgctaa gattattgct
     6001 aaaaatttga attacactta ccttgataca ggtgctatgt atcgttgtgc tacttatctg
     6061 gctttacagc atggatatga agcacaagat gtttcgaaaa ttttaggact cctagctgaa
     6121 agaccgattt cttttggtaa agcagaagat ggcagccaga cagtctttct tggcacagaa
     6181 gaagttactt tagctatccg tcaaaacgat gttactaaca atgtctcgtg ggtttctgct
     6241 atcccagaga ttcgagaaga gttagtaaac cagcagcgtc gtatagccaa agatggtgct
     6301 attataatgg atggtcgtga cataggaaca gttgttttgc cagatgcaga attgaagatt
     6361 tttttagtgg cttctgttga tgaaagagct gagcgtcgct ttaaagaaaa tcaagaaaaa
     6421 ggcattgaga gtgattttga aacactgaaa tcagaaattg ctgctcgtga ttataaagat
     6481 agccatcgag aagtttcacc attaaaggca gcagaagatg ctattgaatt tgatactaca
     6541 ggagtatcaa ttgaaggtgt tgtaactttt attcaagaaa aagcagaaaa aataattgac
     6601 atgaagaatt agtcatgata tactagttgc aatgagaaaa gcagaagtga gaacttctcg
     6661 ccttgcgact taggttgtct ggcccaacat gtcaagattc catctaggaa gattatatgt
     6721 gcgggcttga tttatcaagt ccgctttcgt ttttctctaa aaaaataaag aggtgaagat
     6781 catagctaaa aaagatctat tcattaacga tgaaattcgc gttcgtgaag ttcgtctggt
     6841 tggtctcgag ggtgaacaat taggtattaa accattgtct gaagctcaag caattgcaga
     6901 tgatgctaat gttgatttgg ttttaattca gccacaagct acaccacctg ttgcgaaaat
     6961 tatggactat ggtaagttca aatttgagta tcaaaagaaa caaaaagaac aacgtaaaaa
     7021 acaaagcgtt gttactgtta aggaagttcg tcttagccca gttattgata aaggggattt
     7081 tgaaacaaaa cttcgtaacg gtcgtaagtt ccttgaaaaa ggtaacaaag ttaaagtttc
     7141 tatccgattc aaaggacgta tgattactca taaagaaatt ggagcaaagg tattggcgga
     7201 atttgctgaa gcgacacaag atatcgctat tattgagcaa agagctaaga tggatggtcg
     7261 ccaaatgttt atgcagcttg caccaattcc agacaagaaa taattgtcga ctttatagta
     7321 ttatttaaga ggagaattta aaatgccaaa acaaaaaaca caccgcgcat cagctaaacg
     7381 ctttaaacgc acaggttcag gtggattgaa acgcttccac gcgtttactt cacaccgttt
     7441 ccacggtaaa actaaaaaac aacgtcgtca tcttcgtaaa gctactatgg tttcatcagg
     7501 agatttcaaa cgtattaaag caatgcttac tcgccttaaa taataaggtc tctgtaaaca
     7561 tttactacat attataaagg attatttgga ggaaatataa atggctcgtg ttaaaggtgg
     7621 agttgtttca cgtaaacgtc gtaaacgcgt acttaaatta gctaaaggtt actatggtgc
     7681 aaaacatatc ttgttccgta ctgcaaaaga acaagtaatg aattcttact actatgcata
     7741 ccgtgaccgt cgtcagaaaa aacgtgactt ccgtaaatta tggatcactc gtatcaatgc
     7801 ggctgctcgt atgaatggtt tatcatattc acaattgatg cacggtttga aattagctga
     7861 aatcgaagtt aaccgtaaaa tgcttgctga tttagcagtt aacgatgcag cagctttcac
     7921 agctcttgca gatgcagcta aagctaaact tggtaaataa gaaataaaaa agttgggtct
     7981 gcctaacttt ttttaattac tagaaagaaa gtacctatat tataatttaa gtgaaaagtt
     8041 tcgttctata tatgtagaat gaaacttttt ttgttttatt ccgatacctt tattattgtt
     8101 ataattttcc tttttgattt ttattgtttt atagtattat tccgtataaa aagcacctca
     8161 atcgttggat tgaggtgtgg attaaacttt aaatggtaaa gattattttt tagatgtttt
     8221 atgtgtctta ttaggatctt gcaattccaa gtaacttgga gcgtggaaga gatcaactgt
     8281 tgatttgttg ttatgtttgc tgtatatgct tgttgaacga tctttcaatt tacgttcttt
     8341 agctttgagt gctttaagag aatgtgtgta attgaatttt tcaacttcta cagttttcaa
     8401 accattgttt tcatcaaatc gtagtaaatc tccagtttga atcgaatcac ttgtagataa
     8461 ttgtttatta gtagcatctc gaatagtttt tatttcagct ttagttgttt catcaggatt
     8521 tgtgatttct tgtcctgaat cagtgtaata gagatgtcca ctatagttag tgtatttagg
     8581 agtaatatac tgaccagttg ttctcatagc gaccatttgt ttattatctt tagataacaa
     8641 gtcttgtcct aactgagtat atttagacgt gtctatccct agaatatgaa gtaaggttgg
     8701 taaattatct acctcaccac cgtatgtatt actgataaaa ccttttgaat agccaggaat
     8761 gtggatcata tatggaacac gttgtaacat agcgttgtcg tattcagacc atgtttcagg
     8821 gtctttacct aataattcag ctaagctagg attgcgtgta ttagaaatac cgtagtgatc
     8881 cccatacatt acaataatag aattatcata cacaccagca gcttttagat attcaaaaaa
     8941 tgatttaaga gctgtatcaa ggtagtttgc tgtggcgaaa taaccgttaa tcgtttcatc
     9001 gttagtcttt gcaagtggga atccctcttc gtcagactct cccttcaaac ttgtgtaagg
     9061 gtaatggtta cttactgtaa taaatttagt ataaaacggt tgttgcatgt gctctagata
     9121 tttgatagag tcagcaaaca tatatttatc attcaaaccg tattggaatg aattatcttt
     9181 ggtttgtttt gagaagtagc tagagtcaaa gaagtagtca taaccccact gtttgtaagc
     9241 attatttcta ttccaaaatg ttccgacgtt tccgtggaag accgcactac tgtaacctcc
     9301 attttgagct aagatatgtg gagcagcaaa ttgtgtattt tcaccaccat aattaaccat
     9361 gaatgaacca ctacttagac caaaaagtga gttttccatg agtgtctcag catcggatgt
     9421 cttaccagct ttgacttgat ggaagaagtt agagaaggag atagtctctt tcgaatgata
     9481 aaggctgtta ataaatggag taaccacatg ttctttacca tcaacattta atttatagtc
     9541 aatcaagaat tgttgaaaac tctccaaatg aatcatgata acgtttcgcc cttttccgat
     9601 accataatat tctggattgg gtttggcata atgctctgct acatattttt tggcagtcgc
     9661 taattcttgt gcagtggcac catttctttc tttctgtgct tgataagttt gattaccact
     9721 ataaatcgaa aatgatggca agccaagagc tttaacaata taggtgtttg aaaaaccacg
     9781 acttagtagt tctggacgat caatttcagc taagaataag ttaattgaaa agagtaaacc
     9841 agacaaagct gtgatagaaa aactagcacg tttattaaat ggacgattat ctaagtggat
     9901 tttcttggtt gcaaataaga aaataaggat aatgaaatcg aaaacgtaga caagatccca
     9961 aattctcaat agattaagag cagagtctcc aaggccagcc gaagttttac tactagccaa
    10021 gacagcgcta actgtaatga aatcggaaaa ctctctatag tagattgcgt ttgcaattag
    10081 aaggatattt acgatagcat aggtgataaa agcggtaata taaaaagctt tggttcgctt
    10141 aacatataag gccaaaccta tgattaagag tcctaagggg atggggttta taatggtcaa
    10201 aaacaactga taggaatttt ctaaacctaa attaaaatca gtatggtaag cccaaattgt
    10261 ctttaaccaa tatataacta ctaacaataa aataaagccg agtcgtgtat tgatcgcacg
    10321 gctagcaaaa tctttaattt ttttcacagt aggaacttcc tttaattttt ttcctaaaga
    10381 taaaatctca gattaaatta taacataaaa ttttgtaaca tacggtaaag aaaggtcgaa
    10441 ttcagtatga aaaaagataa aaatttgtta taatactgtt tatgaataaa cttaaagtga
    10501 attctgtcgt tgaaagaaaa atcaaatcag gtgctcagtt actggaaaaa aaagattttg
    10561 ataccagttt agttaaccag ttggttcaac ttttttcaca gtcaaatcaa ttcttaggga
    10621 tggcctatct ttcaccacaa aataaaggga ttggttggtt actatcaaga caaatttttg
    10681 attttaacca tgattacttt gtatcgctat tcgaaaaatc tagagagaag cgtcaaaaat
    10741 ttgaaaaatc tagccaaaca acagcctacc gcttgtttaa tcaggatgga gataactttg
    10801 gtggcctaac tatagatttt tatagtgact atgctctttt ttcgtggtac aatgaatttg
    10861 tttatactaa tcgacaaatg attgttgcag cctttaagca ggtctatcct aatattaaag
    10921 gggcatatga aaaaattcgt ttcaaaggtt tagactttga aagtgctcat ttgtacggtc
    10981 aagaggctcc tgaatcattt ttgattttag aaaataatat caaatatagt gtctttttga
    11041 atgatgggtt aatgacaggt attttccttg accaacatga tgtcagaaaa gccttagcaa
    11101 ctaatctatc agaaggtaaa aaagttctaa atatgttttc atacactgcg gctttttctg
    11161 tagctgcagc tgttggagga gcattagaga caacttctgt tgatttagca aaacgctcgc
    11221 gtgaactttc aaaagcacac tttgatgcta atcagattgt cacagataac catcgattta
    11281 tcgttatgga tgtttttgaa tattataagt atgccaaaag aaaacatcta tcatatgatg
    11341 tgattgttat tgatccacca agttttgctc gaaataaaaa acaaactttt tcggttacta
    11401 aagattacta taaattaatt gaacaagctt tagatatttt agcccctggt ggaactatca
    11461 ttgcatcaac taacgcagct aatctaaccg tatcacaatt taagaaacaa ttggaaaagg
    11521 ggtttggtaa agcttcacat aattacatta gcttacagca gttgcctgaa gatttcactg
    11581 taaatgataa ggaccaacaa agtaattatt taaaagtatt tacaataaag gtaaaataat
    11641 gaaaatagta gtaccagtaa tgcctcgcag tcttgaagag gctcaagaaa tagatttatc
    11701 aaaatttgat agtgttgata ttattgaatg gcgagctgat gccttaccaa aggatgacat
    11761 tattaatgta gctccagcta tttttgagaa attcgcaggt catgaaatta tttttactct
    11821 tcgtacaacg cgtgaaggtg gtaatattgt cttatctgat gctgagtatg ttgagttaat
    11881 ccagaaaatt aattctatct acaatccaga ttatattgat tttgagtatt tttcacataa
    11941 agaagttttt caagaaatgc tagaatttcc aaatttagtc ctgtcttatc acaattttca
    12001 agagacaccg gagaatatta tggagatatt ttcagaatta acagccctag caccacgagt
    12061 tgtgaaaatc gcagtaatgc caaagaatga acaagatgtc ttagacgtta tgaattacac
    12121 tcgcggtttc aagactatta atcctgatca agtttatgcg acggtatcta tgagtaaaat
    12181 tggacgtatt tctcgttttg ctggtgatgt aactggatct agttggacat ttgcatattt
    12241 agattcatct atcgcacccg gacaaattac tatttcagag atgaagcgtg tcaaagcatt
    12301 gcttgacgct gactgatacg agataacaaa atgttaaata tactgatgag atccctagca
    12361 attgcttagg gtttttgtct tattaaaaca aacatgagag gacattagaa tgcaggttga
    12421 agttgactta ccgaatcacc cttatcatat taaaattgaa gaaggatgtt tttctgaggc
    12481 tggtgactgg gtatcacatt tatggcagaa acaaatgata actattatta cagatagtaa
    12541 tgtagaaata ttatacgggg agtcgcttgt caaccagtta aaaaaacaag gttttacagt
    12601 acatgttttt agttttgctg cgggtgaagc tagtaaaact ttggaagttg ctaatcgtat
    12661 ttatggtttt ttagcaaagc atcatatgac cagaagtgat gggatcattg cacttggtgg
    12721 aggcgtcgtc ggagatttag cagcgtttgt tgcttctact tatatgcggg gaatccattt
    12781 tcttcaaatt ccaactagtt taacagcaca ggttgattct tccattggcg gaaaaacagg
    12841 agtcaacact tcattcgcta aaaatatggt aggtactttt gcacaacctg atggtgtact
    12901 gatagatcct gttactctaa aaacattggg caatcgtgaa ttagttgaag gtatgggaga
    12961 agtcatcaaa tatggcttga ttgatgatat aaaactctgg catatcttag aagagatgga
    13021 tgggtctatt gatagtatcc ttgataatgc attagctatt atttatcact cttgtcaagt
    13081 taaacgaaag catgttttag ctgatcaata tgataaaggg ttaaggatgc atttaaattt
    13141 tggccacact atcggtcatg ctatcgaagt acatgctggc tatggagaaa taatgcatgg
    13201 agaagctgtt gcaattggaa tgatccagct ttctcgtgtg gctgagcgta aaaatcttat
    13261 gccaagaggt atcagtcaag atatttataa tatgtgttta aaatttggcc ttccggtcca
    13321 ctatgcagaa tgggataagg acgtcttatt taacatattg agtcatgata aaaaagcaag
    13381 tggacaattt attaaaattg ttatactgcc tcaattaggt agtgcaacag tccaccaaat
    13441 acctttagaa gaaatgagag attatttgga gaaatagatg agatatttaa ctgctggtga
    13501 gtcacatgga cctagtttaa cagctattat cgaagggatt cctgcgggac tgaaattatc
    13561 tgctaaagat attaatgaag atttaaaacg ccgccaaggt ggttatggtc gtgggaatcg
    13621 aatgaaaatt gaaactgatc aagtgatcat tagttctggt gtgcgccatg gaaaaacctt
    13681 gggttcacct attacactga cagtaactaa taaggatcat agcaaatggc tggacatcat
    13741 gtcagtcgaa gacattgaag agcgtctaaa gcaaaaacgt cgtattaagc atcctagacc
    13801 tgggcacgca gatttagtag gtggaatcaa ataccgattt gatgatttga gaaatgcttt
    13861 agagcgttct tcggctcgtg agaccacaat gcgagtagct attggtgcga ttgctaaacg
    13921 tatcttgaaa gaaattggta tcgaaattgc taaccatatt gtagttttcg gtggcaagga
    13981 gattactgtc cctgataagc taacagttca acaaatcaaa gtgttatcta gtcaatcgca
    14041 agtagctatc gtcaatccat catttgaaca agaaattaag gattatattg acagtgtgaa
    14101 aaaggctgga gatactatcg gaggagtgat tgaaactatc gttggaggag ttcccgttgg
    14161 tttaggctct tatgtacact gggatagaaa gttagatgct aagatagcgc aagcagttgt
    14221 atctatcaat gcttttaaag gtgttgaatt tggtctaggt tttaagtcag ggtttttaaa
    14281 aggaagtcaa gtaatggact ccatatcatg gacaaaggac caaggttaca tacgtcagtc
    14341 aaataattta ggcggttttg aaggcggtat gactaatgga gagcctattg tcgttagggg
    14401 agtaatgaag ccaattccta cgctttacaa gccactaatg tcagttgata ttgatactca
    14461 cgagccttat agagctacag tggaacgttc tgatcccacg gcacttccgg ctgcaggtgt
    14521 cgttatggaa gctgtagtag ccaccgtcct ggtgacagaa gtgctagaaa aattttcttc
    14581 agacaacatg tatgaactaa aagaagctgt aaaactatat cgcaattatg ttaatcattt
    14641 ttaagtgaaa actagtagca ttcaattgca aaaaaaggta taaaagtcta caatagtggt
    14701 aagttcaagt agaaaaagga gtaacttatg gctaatgtat atgatttagc aaatgaatta
    14761 gaacgtgctg ttcgtgcttt accagaatac caagcagttt taactgcaaa agcagctatt
    14821 gaaaatgatg cggatgcaca agtgctttgg caagactttt tggctaccca atcaaaagtt
    14881 caagaaatga tgcaatctgg ccaaatgcca agtcaagaag aacaagatga aatgtctaaa
    14941 cttggggaaa aaattgaatc caatgacctt ttaaaagttt attttgacca acaacaacgg
    15001 ttgtctgtct atatgtctga tatcgaaaaa attgtctttg cacccatgca ggacttgatg
    15061 taatagtata aaagagagag ctccttgagt tttctctttt atgctattta cattcagagc
    15121 atcctaattg ctgcgctttt acattattgc attatcctat aaaaatgtat ttttatactt
    15181 tttttgagag cggctattta tttttagact tagtcatgtc tctaaatacc cctagttagt
    15241 gataaaaa
//
LOCUS       DATRUK010000053        14987 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.053, whole genome shotgun sequence.
ACCESSION   DATRUK010000053 DATRUK010000000
VERSION     DATRUK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14987
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.053"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(104..2101)
                     /gene="metG"
                     /locus_tag="VBE34_001510"
     CDS             complement(104..2101)
                     /gene="metG"
                     /locus_tag="VBE34_001510"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEO7264218.1"
                     /translation="MTEKKSFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGFD
                     VQYLTGLDEHGQKIQQKAEEAGITPQEYVDGMAESVKTLWELLDISYDKFIRTTDTYH
                     EEAVAKIFEQLLAQGDIYLDEYTGWYSVSDEEFFTESQLAEVYRDENGNMIGGVAPSG
                     HEVEKVSEESYFFRMSKYADRLKAYYAEHPEFIQPDGRMNEMLKNFIEPGLEDLAVSR
                     TTYTWGVQVPSNPKHVIYVWIDALMNYISALGYGWSDDLSQYHKFWPADIHMIGKDIL
                     RFHSIYWPIMLMALDLPLPKRLVAHGWFVMQDGKMSKSKGNVVYPEMLVERFGLDPLR
                     YYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTIAMVNKYFDGEVPRFAVAT
                     DFDADLASVATDSIENYHKQMEAVDFPRALEAVWNLISRTNKYIDETAPWVLAKDETD
                     RDKLAAVMSHLVASLRVVAHLIQPFMMETSDAIMEQLGLGATFDLEKLTFADLPEGVR
                     VVAKGSPIFPRLDMEDEITYIKEQMNAGKAPVEKEWVPEEVELTSSKGQIKFEDFDAV
                     EIRVAEVIEVEKVEGSDKLLRFRLDAGDEGHRQILSGIAKFYPNEQELVGKKLQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTVLTVDSAVANGSIIG"
     gene            2244..3119
                     /gene="tehB"
                     /locus_tag="VBE34_001511"
     CDS             2244..3119
                     /gene="tehB"
                     /locus_tag="VBE34_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEO7264219.1"
                     /translation="MVNTKTLLPYKTMPVWTAQSIPKAFLEKHNTKEGTWAKLTILSG
                     SLVFYQLSPDGEEISRHIFDASSDIPFVDPQVWHKVSPNSPDLSCYLTFYCQKEDYFH
                     KKYGLTRTHSEVIASAPLLSEKSNILDLGCGQGRNSLYLSLLGHQVTSVDSNGQSLVA
                     LENMALEEELPYNIKRYDINTAAIEGHYDFILSTVVFMFLNPDCISDIILQMQSHTQI
                     GGYNLIVSAMDTAENPCPLPFPFTFKEGQLKSYYNDWEIIKYNENLGELHRVDENGNR
                     LKLQFATLLARKLRS"
     gene            complement(3277..3972)
                     /locus_tag="VBE34_001512"
     CDS             complement(3277..3972)
                     /locus_tag="VBE34_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO7264220.1"
                     /translation="MSKKSHRQYQIYEGLRCAVALCFISGYINAFTYVTQGKRFAGVQ
                     TGNLLSFAIHLSNKHYSQALAFLLPIMVFMLGQSFTYFMNRWANKHQLHWYLLSSFAL
                     TQVAIVTIILTPFLPSSFTVAGLAFFASIQVDTFKSLRGAPYANMMMTGNIKNAAYLL
                     TKGLYEKNSDIFLIARNTIIIIGGFIFGVVCSTYFSSKLGEWSLSLILIPLLYVNLLL
                     GHEFYNLQVERHS"
     gene            4308..5525
                     /locus_tag="VBE34_001513"
     CDS             4308..5525
                     /locus_tag="VBE34_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7264221.1"
                     /translation="MHSTIYKCHHCRYLIYKSFKDIFPFALIFSWIEILQKLVISDDS
                     FLAQTTKSSIYKNEWLYTVSNTLYDLSHLLIYFMASYFVASLVWRGLSNQTKESFSAS
                     LLGFATVWLLLTSVVPGTDYHYPSQPIWLMLALISYLFIFLTRLLQNRHHIFQYSMLA
                     ILLLISSILSHILYSYPNIDLEYLIQNHFSQWLGDGPHQLIPIMLWSILAIFLTLSGL
                     FVPDILFRPYSYFSVVSENLNAALSQHTDKIPYLYTFYTVKNSFAMFGGIGILLSLFL
                     AVLYESRKLQSKNYYKLTLLTLTPLIFDQNLPFLVGLPVILQPILFIPMVLTTIFAEA
                     FGALMLYLKFVDPAVYTVPSGTPSLLFGFLASNGDWRYLPVTAIILVVGFFIYRPFVK
                     IAFAKEEQYEKIT"
     gene            5509..6351
                     /locus_tag="VBE34_001514"
     CDS             5509..6351
                     /locus_tag="VBE34_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7264222.1"
                     /translation="MKKLLRWLPPVLFIIILIGMTILGKSYINKVTAHKIKLYNSRMT
                     PTILIPGSSATQERFNSMLAQLNQMGEKHSVLKLTVKKDNSIIYNGQISGNDHKPYIV
                     IGFENNEDGYSNIKKQTKWLQIAMNDLQKKYKFKRFNAIGHSNGGLSWTIFLEDYYDS
                     DEFDMKSLLTMGTPFNFEESNTSNHTQMLKDLISNKGNIPSSLMVYNLAGTNSYDGDK
                     IVPFASVETGKYIFQETAKHYTQLTVTGNNATHSDLPDNPEVIQYVAEKILKNEKGKL
                     PKPH"
     gene            6428..7255
                     /locus_tag="VBE34_001515"
     CDS             6428..7255
                     /locus_tag="VBE34_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEO7264223.1"
                     /translation="MKLISWNIDSLNAALTSESTRALMSRQVIDTLVAEDADIIAIQE
                     TKLSAKGPTKKHLEVLETYFPEYDLVWRSSVEPARKGYAGTMFLYRKGLNPIVSFPEI
                     DAPTTMDNEGRIITLELENCYITQVYTPNAGDGLKRLADRQIWDIKYAEYLATLDSQK
                     PVLATGDYNVAHKEIDLANPSSNRRSAGFTAEERQGFTNLLAKGFTDTFRYLHGDVPN
                     VYSWWAQRSRTSKINNTGWRIDYWLTSNRVADKITKSEMIHSGDRQDHTPIILEIEL"
     gene            complement(7295..7651)
                     /locus_tag="VBE34_001516"
     CDS             complement(7295..7651)
                     /locus_tag="VBE34_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEO7264224.1"
                     /translation="MYTFYEYPKCTTCRSAKKELTELGLTFEAIDIKSNPPKASLLKE
                     LLENSPYDLKKFFNTSGNSYRELGLKDKFDDLTLDQALDLLASDGMLIKRPLLVKDNK
                     ILQIGYRTKYKDLNLV"
     gene            complement(7653..8129)
                     /locus_tag="VBE34_001517"
     CDS             complement(7653..8129)
                     /locus_tag="VBE34_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEO7264225.1"
                     /translation="MLYQEFYQSPLGEIRLLADNLGLSGLYFVGQKYDMLAVNQEEIV
                     NMSNSYTLLGKKWLDAYFSQQNLPSIPLSLRGTAFQTRVWQELQKIPFGDTKTYGELA
                     KELNCQSAQAVGGAIGKNPISLIIPCHRVLGRYGQLTGYAGGLERKSWLLEYEKEK"
     gene            complement(8184..9334)
                     /locus_tag="VBE34_001518"
                     /pseudo
     CDS             complement(8184..9334)
                     /locus_tag="VBE34_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
     gene            complement(9407..9955)
                     /locus_tag="VBE34_001519"
     CDS             complement(9407..9955)
                     /locus_tag="VBE34_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO7264226.1"
                     /translation="MQIRLAFPNEIDQIMLLIEEARAEIAKTGSDQWQKEDGYPNRND
                     IIDDILNGYAWVGIEDGMLATYAAVIDGHEEVYDAIYEGKWLHDNHRYLTFHRIAISN
                     QFRGRGLAQTFLQGLIEGHKGPDFRCDTHEKNVTMQHILNKLGYQYCGKVPLDGVRLA
                     YQKIKEKGETSIYREIDERNPM"
     gene            complement(10023..11114)
                     /gene="serC"
                     /locus_tag="VBE34_001520"
     CDS             complement(10023..11114)
                     /gene="serC"
                     /locus_tag="VBE34_001520"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEO7264227.1"
                     /translation="MIIYNFSAGPAVLPKPVLVKAQSELLNYQGSSMSVLEVSHRSKE
                     FDDIIKGAERYLRDLMGIPDNYKVIFLQGGASLQFSMIPLNITRGRKAYYHVAGSWGK
                     KAYTEAVKLSKTIPFEPILLASSEESVYDYIPEFDEKEIDPKAAYVHVTTNNTIEGTS
                     LYDIPKTNGVPVIADMSSNILAVKYKVEDFAMIYAGAQKNIGPAGVTVVIIREDMINE
                     EPTLSSMLDYKVQSDAGSLYNTPPAYSIYIAKLVFEWVKSLGGVDAMEKANREKSGLL
                     YDYIDSSEFYSNPVRDKKSRSLCNIPFITINKDLDEKFVKEATERGFKNIKGHRSVGG
                     MRASLYNAFPKQGVIELIDFMKTFEAENT"
     gene            11247..11882
                     /locus_tag="VBE34_001521"
     CDS             11247..11882
                     /locus_tag="VBE34_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEO7264228.1"
                     /translation="MILREFCAENLTDLTRLDKAIISRVELCDNLAVGGTTPSYGVIK
                     EANQYLHEKGISVAVMIRPRGGNFVYNDLELRIMEEDILRAVELESDALVLGILTSNN
                     HIDTEAIEQLLPATQGLPLVFHMAFDVIPKSDQKKSIDQLVALGFTRILLHGSSNGEP
                     IIENIKHIKALVEYANNRIEIMVGGGVTAENYQYICQETGVKQAHGTRITQ"
     gene            complement(12152..13021)
                     /gene="rsmI"
                     /locus_tag="VBE34_001522"
     CDS             complement(12152..13021)
                     /gene="rsmI"
                     /locus_tag="VBE34_001522"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEO7264229.1"
                     /translation="MEMQVQKSFKSNIHYGTLYLVPTPIGNLDDMTFRAIRILREVDF
                     ICAEDTRNTGLLLKHFDITTKQISFHEHNAYDKISGLIDLLKEGKSLAQVSDAGMPSI
                     SDPGHDLVKAAIEGDIPVVSIPGASAGITALIASGLAPQPHIFYGFLPRKKGQQITFF
                     ETKQDYPETQIFYESPFRVSDTLKHMKEIYGDRQVVLVRELTKLYEEYQRGTISQLLE
                     HIEKVPLKGECLIIVDGKRDTERVKDSSQQDPLVLVKEYIANGDKTNQAIKKVAKEFN
                     LNRQELYASFHDL"
     gene            complement(13021..13347)
                     /gene="yabA"
                     /locus_tag="VBE34_001523"
     CDS             complement(13021..13347)
                     /gene="yabA"
                     /locus_tag="VBE34_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEO7264230.1"
                     /translation="MDKKDLFDAFDDFSQNLLVGLSEIETMKKQIQKLLEENTVLRIE
                     NGKLRERLSVIEAETETAVKNSKQGRELLEGIYNDGFHICNTFYGQRRENDEECAFCI
                     ELLYRD"
     gene            complement(13377..14240)
                     /locus_tag="VBE34_001524"
     CDS             complement(13377..14240)
                     /locus_tag="VBE34_001524"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEO7264231.1"
                     /translation="MDLKRTQPKLLEKFNTILQSDRMSHAYLFSGNFASLDMALYLAQ
                     SQFCEKRQSGLPCQECRACRLIANGEFSDVKIIEPQGQLIKIETIKELTKDFSRSGFE
                     GKSQVFIIKYCEKMHVNAANSLLKFIEEPQSSSYVILLTNDENNVLPTIKSRTQIFRF
                     PKQLDMLVHQAEQAGLLKSQASLLAQVADDPKHLEILLTNKKLLDYLNLSQQFVTTLA
                     KDRQTAYLEVSRLTSQVVDKNDQAFVFQWLTIMLAKEGQLYDLENTYRAQQMWKSNVS
                     FQNSLEYMVLS"
     gene            complement(14260..14895)
                     /locus_tag="VBE34_001525"
     CDS             complement(14260..14895)
                     /locus_tag="VBE34_001525"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEO7264232.1"
                     /translation="MKKGLMISFEGPDGAGKTTVLEAVLPLLREKLSQDILTTREPGG
                     VTISEEIRHIILDVKHTQMDKKTELLLYMAARRQHLVEKVLPALEEGKIVLMDRFIDS
                     SVAYQGSGRGLDKSHIKWLNDYATDSHKPDLTLYFDVPSEVGLERIQKSVQREVNRLD
                     LEQLDMHQRVRQGYLELADSEPNRIVTIDASQQLDEVIAETFSIILDRINQ"
BASE COUNT         4758 a         2853 c         2376 g         5000 t
ORIGIN      
        1 ttatatcaaa aataaaaata aattctaggt cattttcata attttccaac tattcagtca
       61 cttttttctt gaaaacaaaa aaagagcata atgctctttt cgattaacca ataattgatc
      121 catttgccac tgctgaatcg accgtcaaca ctgtcaattt accatcatgt tctgctgaga
      181 gaatcatgcc ttgactgaca tattttttca tcattttacg tggtttgagg ttagcaacaa
      241 tttggagttt tttccctact aactcttgtt catttgggta aaattttgca atccctgata
      301 agatttgacg atggccctca tcaccagcat ctagacggaa acgaagaagt ttatcagaac
      361 cttccacttt ctcaacttca attacttctg caacgcggat ttcaacagcg tcaaaatcct
      421 caaacttgat ttgacctttg cttgatgtca attcaacttc ttcaggaacc cactcctttt
      481 ccactggtgc ttttccagca ttcatttgtt ctttgatata agtaatttcg tcttccatat
      541 ccaaccttgg gaaaattggt gaaccttttg caacaactcg aacaccttct ggtagatcag
      601 caaaagtcaa tttttcaaga tcgaatgttg cccctaaacc aagttgttcc ataatggcat
      661 cggaagtttc catcatgaac ggttgaatga ggtgagcaac aacacgtaga ctagcaacta
      721 aatgactcat tacagctgct aacttatcac gatctgtttc gtcttttgca agtacccacg
      781 gtgctgtttc atcaatatat ttgttagttc gtgaaataag attccataca gcttcaaggg
      841 cacgtgggaa atccaccgct tccatttgtt tatgatagtt ttcaatacta tctgtcgcaa
      901 cagaagctaa atcggcatca aagtcagttg caacagcaaa ccttggtact tctccatcga
      961 aatacttgtt aaccatagca atagtgcggt tgagaaggtt tcctaaatca ttagcaagtt
     1021 catagttaat acgtccaaca taatcttctg gcgtaaaagt tccgtctgaa ccaacaggca
     1081 atgaacgcat gagataataa cggagtggat caagaccaaa acgctctact aacatttctg
     1141 gataaacgac attccctttt gatttagaca ttttaccatc ttgcatcaca aaccaaccat
     1201 gagcaactag acgttttggt aatggcaaat ccagcgccat caacatgata ggccaataaa
     1261 tagagtggaa acgaagaata tctttaccaa tcatatgaat gtctgctggc caaaatttat
     1321 gatattgact gagatcgtca gaccaaccat aaccaagagc agaaatatag ttcatcaaag
     1381 catcaatcca gacataaata acatgttttg gatttgatgg tacctgaact ccccatgtgt
     1441 aagttgtacg agacactgct aaatcttcta aacctggttc tataaagttt ttaagcattt
     1501 cattcatacg accatctggc tgaataaatt ctggatgctc agcgtagtac gcttttaaac
     1561 gatctgcata tttgctcatg cggaagaaat aagattcttc agaaactttt tcaacttcat
     1621 gaccagatgg cgcaacacca ccaatcatat taccgttttc gtcacgataa acttctgcta
     1681 attggctttc agtaaaaaat tcttcatctg aaacagaata ccatccggta tactcatcaa
     1741 gatagatgtc cccttgtgcc aacaattgtt caaaaatttt agcaaccgct tcttcatggt
     1801 aagtatctgt tgtacggatg aatttgtcat aggaaatatc taaaagttcc cataatgttt
     1861 tgacactttc agccatgcca tcaacatatt cctgtggtgt aatgcctgct tcttcagcct
     1921 tttgttgaat tttctgacca tgctcatcaa gaccagtcaa atactgaaca tcaaagccca
     1981 tcatgcgttt gtaacgtgcc aaaacatcac atgctatcgt tgtataggca gaaccgatgt
     2041 gcaatttacc tgatgggtaa taaattgggg tagtaatgta aaatgatttt ttttctgtca
     2101 tgatttctcc tataagccac cctaatgaga ggtgtttttt atcatgatac attataacat
     2161 aaaatctttt tcaactggat atatctctga ataatatatc tacttttgct ataataaagg
     2221 tattaaaaat agagagttaa tatatggtta atacaaaaac acttctcccc tacaaaacta
     2281 tgccggtttg gactgcacag tctattccaa aggcattttt agaaaagcat aatactaagg
     2341 aaggcacctg ggcaaaacta accattctaa gtggttcttt agtattttac cagttatctc
     2401 ctgatggaga ggaaatctcg cggcatattt ttgatgctag tagtgatatt ccttttgttg
     2461 atccacaagt ctggcataaa gtttcgccga atagtccaga cttaagttgc tatctaactt
     2521 tttactgcca aaaagaagat tacttccata aaaaatatgg tctcacgcgc acacattctg
     2581 aggttatcgc cagtgcacct ctcttatctg agaagagtaa tatattagac cttgggtgtg
     2641 gtcaagggcg aaactcactt tatttatcgc tgctgggaca tcaagtgact tctgtcgatt
     2701 caaacggaca gagccttgta gctttagaaa atatggcatt agaagaagag cttccttaca
     2761 atataaaaag gtatgatatt aatactgctg ctattgaagg gcactatgat tttattttat
     2821 caactgtggt atttatgttt ttaaatcccg actgcatttc tgatattatt ttacaaatgc
     2881 aatcacatac tcagattgga ggatacaatc ttatcgtttc tgctatggat accgctgaaa
     2941 atccttgccc gcttccattc ccttttacct tcaaagaagg acaattgaaa tcttattaca
     3001 atgactggga aatcatcaaa tataatgaaa atttaggtga attgcacagg gtggatgaaa
     3061 acggtaatcg cttaaaatta cagtttgcga ctttattagc tcgaaaatta agatcataaa
     3121 agtgagtaga aatctactca cttttatctt tggttaggta ataagtatat tctaaaccca
     3181 tacataatta tcctataaaa tatatagaga tgatatttca aatcattatt gtaacaatca
     3241 gctttagtat ggctcttcaa cataaaacgc catacattag gagtgccttt ctacttgtag
     3301 gttataaaat tcatggccta acagcaagtt gacatataaa agaggaatta atattaatga
     3361 taagctccat tcgcccagtt tacttgaaaa ataagtcgag caaacaacac caaagataaa
     3421 tcctccaata ataataatcg tatttctagc aattaagaaa atatcagaat ttttttcgta
     3481 aagtcccttt gttaataaat aagcagcatt cttgatatta ccagtcatca tcatattagc
     3541 ataaggtgct cctctgagcg atttaaaggt atctacttgt attgaagcaa agaaggctag
     3601 tcctgccaca gtaaacgacg atggtaagaa aggagtcaat ataattgtta ctatagcaac
     3661 ctgtgtcaac gcaaaactag ataataggta ccagtgcagt tgatgtttat tcgcccaacg
     3721 attcatgaaa taagtaaacg attgacctaa catgaaaacc attataggta gcaaaaaagc
     3781 aagcgcttga ctataatgct tattactaag atgaatagca aaagataaca aattccctgt
     3841 ttgtacacct gcaaaacgtt ttccttgtgt tacataagta aaagcattga tataacctga
     3901 aataaaacag agtgcaaccg cacacctgag cccttcatat atttgatatt gacggtgaga
     3961 ctttttagac atattttcct ctaactttat ggcaaacaaa aataagactg ggcaatcttg
     4021 ctcagcactt tttttctaat agattggttt aaaatagtat taatattagc actagctagc
     4081 ctaacagttc taaataaatt attaagcaat gataaatgta gttttagtat atcatagcct
     4141 cagcattgaa gcaatctcac tttatgaaag gataaagaaa atctcataag tcatttaccc
     4201 ttaatttgaa gtttaattag ttacttttaa ttttactacc aaaaaacttg caccattctt
     4261 aacatttgtt acaataaata caacctatta ggaggtcagt acagattatg cacagcacta
     4321 tttataaatg tcatcattgc cgttatctaa tttataaaag ttttaaagat atctttcctt
     4381 ttgctctcat tttttcttgg attgaaatcc ttcaaaaact cgttatatca gatgattctt
     4441 ttttagctca aacaacaaag tccagtattt ataaaaacga gtggttatat acggtttcca
     4501 atactcttta tgatttatct catttactaa tctactttat ggctagttat tttgtggcta
     4561 gtttagtatg gagaggctta tctaaccaaa ctaaagaaag tttttctgca agtttacttg
     4621 gctttgccac tgtttggttg cttctaactt cagttgttcc aggtacagat tatcactatc
     4681 ctagccaacc catttggctt atgctggctt tgatttcgta tctttttatc tttttaactc
     4741 gactgcttca aaatcgacat catattttcc agtatagtat gttagctata ctactattaa
     4801 tctcttctat tttgtcacat atcttatata gttatcctaa tattgattta gaatacctta
     4861 tacaaaatca cttttctcaa tggctgggtg atgggccaca tcaattaatt cctattatgc
     4921 tttggtctat cttagctatt tttctaacct tatcaggtct ttttgtacca gatattttat
     4981 ttcgtcctta ctcctatttt tcagtagtta gcgaaaattt aaatgctgcc ttgagccaac
     5041 atacagataa aatcccttat ctgtatacct tttatactgt taaaaatagc tttgctatgt
     5101 ttggcggtat tggaatccta ttatccttat ttttagcagt tttatatgaa agtagaaaac
     5161 tacaatcgaa gaattactat aaattgacat tactgacctt aactcctctt atttttgatc
     5221 aaaatttacc atttttagta ggtctacctg tcattttaca accaatatta ttcattccga
     5281 tggtattaac aactatcttt gcagaggctt ttggagctct tatgctctac ctaaaatttg
     5341 tcgaccctgc agtttatact gtcccaagtg gtacacctag tctactattt ggctttcttg
     5401 cttcaaatgg ggattggcgt taccttccag tcactgcaat tattctagtt gttggcttct
     5461 ttatttatcg tccgtttgtc aaaatcgctt tcgctaagga ggagcaatat gaaaaaatta
     5521 cttagatggc ttcctcctgt acttttcatt attatcctta taggaatgac tatcttaggt
     5581 aagtcctata tcaataaagt aacagctcac aaaataaaac tctataactc tcgaatgact
     5641 cctactattt taattccagg atccagtgct actcaagaac gatttaacag catgttagca
     5701 cagctcaacc aaatgggaga aaaacatagc gttttaaagt taactgtcaa aaaagacaat
     5761 agcattatct acaatggaca aattagcggc aatgaccaca aaccctacat tgtcattgga
     5821 tttgaaaata atgaagatgg ttatagtaac atcaaaaaac aaacaaaatg gctacagatt
     5881 gctatgaatg atcttcagaa gaaatataaa tttaaacgtt ttaacgctat cggtcattca
     5941 aatggtggct tatcatggac tattttccta gaagattatt acgactctga tgaatttgat
     6001 atgaaatcat tgttaacaat gggaacacct tttaactttg aagaaagtaa cacctcaaat
     6061 catactcaaa tgcttaaaga tttaatcagt aataaaggaa atattccatc aagtctcatg
     6121 gtatacaatt tggcaggaac taattcatat gatggtgata aaattgttcc atttgctagt
     6181 gtggagactg gtaaatatat tttccaagaa accgctaaac actataccca actaacagta
     6241 actggtaata atgctacaca ttctgacttg cctgataatc ctgaagttat ccaatatgtc
     6301 gcagaaaaaa ttcttaaaaa tgagaaaggt aaattaccaa aacctcacta aagctgtaga
     6361 atagcaacgc ctctggtatt ttgatataat atattgcgaa ttaaactaaa taaaaaagga
     6421 tattattatg aaactcattt catggaatat tgattccctc aatgcggcat taacaagtga
     6481 atcaacacgt gctttaatgt cacgccaagt tattgatact ttagttgctg aagatgccga
     6541 catcattgcg attcaagaaa ctaaactgtc tgccaaagga cctactaaaa aacatttgga
     6601 agtacttgaa acttacttcc cagaatatga tttggtttgg cgatcatcag tcgaacctgc
     6661 tcgtaagggc tatgctggta caatgttctt atatcgtaaa gggttaaatc ctattgttag
     6721 cttccctgaa atcgatgctc ctactacaat ggataatgaa ggtcgcatta ttactctcga
     6781 attggaaaac tgctatatca cacaggtcta tactccaaat gcaggtgatg gcctaaagcg
     6841 cttagctgac cgtcaaatat gggatattaa atatgctgag tatttagcaa cattggatag
     6901 tcaaaaacct gttctagcaa caggtgacta taacgtagcc cacaaggaaa tagacctagc
     6961 taatcctagc agtaatcgtc gttctgctgg attcacagct gaagagcgcc aaggctttac
     7021 aaatctacta gccaaaggtt ttacagatac ttttcgttac cttcatggcg atgttccaaa
     7081 tgtttatagc tggtgggcac aacgaagtcg tacatccaaa atcaacaata cagggtggag
     7141 aattgattat tggctaacaa gtaaccgtgt cgcagataaa attaccaagt cagaaatgat
     7201 tcattctggt gatcgtcaag atcatacacc aattattttg gagattgaac tataaaccaa
     7261 aaaaagatga ttgagttata actcaatcat ctttttatac taagttgagg tctttatact
     7321 tagtacggta gccaatctga agaattttat tatctttaac tagcaatgga cgcttaataa
     7381 gcattccatc cgatgccaat aagtccaaag cttggtcaag agttaaatca tcaaatttat
     7441 cttttaagcc taattctctg taagaatttc ctgaagtatt gaaaaacttt ttaaggtcat
     7501 aaggcgaatt ctctaacaat tcttttaata acgaagcttt gggaggattg ctttttatat
     7561 caatcgcttc aaaagtcaag ccgagttcag ttaactcttt ttttgctgaa cgacaagtgg
     7621 tacatttggg gtattcataa aacgtataca tattattttt ccttttcgta ttctaataac
     7681 caagattttc tttctaagcc accagcataa ccagttaatt gaccatatct tcctaaaaca
     7741 cggtgacaag gtataattag agaaatagga tttttaccta ttgctcctcc aacagcctgt
     7801 gcagattgac aatttagctc tttcgctaat tctccataag ttttggtatc tccaaaaggg
     7861 attttctgta attcctgcca tactctcgtc tgaaatgctg tccctctcaa tgataatgga
     7921 atactaggta aattttgctg tgaaaaataa gcatctaacc acttcttacc aagtaaagta
     7981 taggaatttg acatattaac aatttcctct tggttgactg ctagcatatc atatttttgc
     8041 ccaacaaaat aaagccctga taaacctaga ttgtctgcta gaaggcgaat ttctccaaga
     8101 ggggactgat agaattcttg atataacata tgataagcat aacttaaaaa gccatgaaaa
     8161 gcaattcatg actttttagt tctttattgt tgttttgcaa ttaaacgtac acggacgata
     8221 ttctcatcac cttcaaattc ttcaattaaa gtagaaatct tgttattatc cgtttcatct
     8281 aaatctatta gtgtgtaggc gtagtcaccc tttgaacggt taataatgtt atcaatattt
     8341 atacctaaca ctgatacagc agttgaaatt ttagccataa tgttaggaac attcttgtta
     8401 attaaagtaa tacgaaatgg tgcggtttga atttgatgta cattggggaa attaactgaa
     8461 ttagtgattt ctcctgtctc cataaaacaa cgaatggttt gacttgccat aatagcacaa
     8521 tttagctctg cctcatctgt tgatccgcct acatgaggaa agactgtaat tcctttttgg
     8581 tttaataatt ctttgtctcc aaaatcagta atatagcgct tgacaacacc agtttctatc
     8641 gcttcaaata gctcttggtt attaactaat tctgcacgag caaagttgat aatcgtagtt
     8701 ccttttttca tgattgaaaa agcttccgca tcaaaagtat gcttagtttc atttgttaag
     8761 ggaacatgaa ttgtgatata gtcacaagtt tcaaaaatat ccttaatctc tttaaccctt
     8821 tgaacatggc ttgaaatatt ccaagctgtt tcaattgaaa tatagggatc ataaccaaga
     8881 actgtcattc ctaagcgtct agcacattcg caattctagc tccaatggca ccaaggccga
     8941 taactcccaa tttttttcct gcaatttcat taccagcaaa agctttcttt cctgcttcaa
     9001 tttgtttggg aatatctgtt ccagttagag tattaaccca tcggttagct cctaaataat
     9061 cacgagctga aagtaataag gcagcaatga ccgcttcttt tacagcatta gcatttgcac
     9121 ctggggtatt aaacacgact attccctgtg cacttgcctc ttcaatagga atattatttg
     9181 ttcctgcacc cgccctagca atagctttga agttacttgg gaaatcctga ttatgcaaat
     9241 tttgactacg aataatacag gcatcaggat tttctgacat atccccatca atctggaaac
     9301 gattccctaa ctcttgtaaa ccaatttgat taatattatt aaatgttttg acactaaggt
     9361 tatctcttta gtcatcattg accaaaataa cccctttctt tttttcttac atgggatttc
     9421 tttcatcaat ctcacgatag atgctcgttt ctcccttttc ttttatcttc tgataagcca
     9481 atcgtacccc atctagaggt actttaccac aatattgata gcctaattta tttagaatat
     9541 gctgcatagt gacatttttt tcatgagtat cgcaacgaaa atcaggtcct ttatgacctt
     9601 caattaaccc ttgaaggaat gtctgtgcta gtcctctacc acgaaattga tttgagatcg
     9661 caatacgatg aaatgtaaga tagcgatggt tatcatggag ccattttccc tcataaatag
     9721 catcataaac ctcttcatga ccatcaatga cagccgcata agtcgctagc atcccatctt
     9781 caataccaac ccaagcataa ccgttcaaga tatcatcaat aatatcgtta cgattagggt
     9841 atccatcttc tttttgccat tgatcacttc ctgttttagc aatttcggca cgcgcttcct
     9901 caataagaag cataatttgg tcaatttcat ttggaaaagc taatctgatt tgcattatat
     9961 tacccacttt tatatattat agactataat aacggtttaa ttttctttcg tgataacatt
    10021 tactatgtat tttctgcttc aaatgttttc ataaaatcaa ttaattcgat gacaccttgt
    10081 ttaggaaagg cattataaag gctagctctc ataccaccta ccgaacgatg tcctttaata
    10141 tttttaaatc cacgttctgt cgcttccttt acaaattttt catctaaatc tttattaata
    10201 gttatgaaag gaatgttaca tagagaacga ctctttttat ctctgacagg attactataa
    10261 aattctgagg aatcaatata atcatagaga agtcctgatt tttcacggtt agctttttcc
    10321 atagcatcaa caccacctag acttttcacc cattcaaaga ctaatttagc aatgtaaata
    10381 ctgtaagcag gcggcgtatt ataaagagaa ccagcatctg actgaacctt ataatccaac
    10441 attgatgata aagttggttc ctcattaatc atatcttcac ggataataac taccgttaca
    10501 ccagcaggtc caatattttt ttgagctcct gcataaatca ttgcaaaatc ttctacttta
    10561 tattttacag ctaatatatt agaagacata tccgcaataa caggcactcc attagtttta
    10621 ggaatatcat aaagcgatgt tccttcaatg gtattatttg ttgtcacatg aacataggca
    10681 gctttaggat caatctcttt ttcatcaaac tctggaatat aatcataaac tgattcttct
    10741 gatgaagcta atagaattgg ttcaaaagga attgtttttg atagttttac agcctctgta
    10801 taagcttttt tcccccacga accagcaaca tgatagtaag ctttacgccc tctggtaata
    10861 ttcaagggaa tcatactaaa ctgtaaggat gcaccacctt gtaaaaagat tactttataa
    10921 ttatccggaa ttcccatcaa atctcttaag taacgctcag cacctttgat aatatcatca
    10981 aactctttgg agcggtgcga tacctccaaa acactcatac tagacccttg ataattaagg
    11041 agttcagatt gggcttttac aagcactggt tttggtaaga ctgcaggacc tgcagaaaaa
    11101 ttataaatta tcatataaca cctccatata attgtgtaag attatagcac agtattttga
    11161 atattgtaaa gatttttttg attaattttg ctttttattc gtgatataat ggtaaaggcg
    11221 aacaatcaag tttaaaggag taatttatga ttttgaggga attttgtgca gagaatttaa
    11281 cagacttaac cagattagat aaagctatta tttcacgtgt cgagctttgt gataaccttg
    11341 ctgtcggagg cactactcct agctacggtg ttatcaaaga agctaatcaa tatttacatg
    11401 aaaaaggtat tagtgtcgct gttatgattc gtccccgtgg aggaaatttt gtatacaatg
    11461 atttagaatt acgtattatg gaagaggata tcctacgtgc tgttgagtta gaatcagatg
    11521 ctcttgtttt gggaatatta acttcaaata atcatattga tactgaagcc attgaacaac
    11581 ttctacctgc tacccaaggt ttgccacttg tttttcatat ggcatttgat gttatcccga
    11641 aatctgacca aaaaaaatca atagatcaat tagttgcttt aggctttaca aggattctcc
    11701 tacatggttc atctaatggc gaaccaatta tagaaaatat caaacatatc aaagcattag
    11761 ttgaatatgc taataatcgt attgaaatta tggttggcgg cggtgtgact gctgaaaact
    11821 atcaatatat ttgccaagaa actggggtta aacaagccca tggtactaga ataactcaat
    11881 aaaaaattcg aaacagggct ataagtttct ttagtatatt tactactaag ttctctcatg
    11941 ttttgtaaaa cctgaaagaa ccggaataag agcataccat cttttataac ctactctcag
    12001 aaataaaatc caatttgcaa tggtattcaa aagagctgaa attaaaaatt ttaaggaaat
    12061 tggaagtatc ttacttcttt attccaatag ctaaatcgga gattataaaa ggttgccaaa
    12121 tttttctttg gcaacctttt tatattatta cttataaatc atggaaacta gcatagagtt
    12181 cttgtctatt gagattaaat tcttttgcta ctttttttat cgcttgatta gttttatcac
    12241 cattagcgat atattctttt actaatacta gtggatcttg ttggctactg tctttcactc
    12301 gctcggtatc tctcttacca tcaacaataa ttaagcattc acctttgaga gggacctttt
    12361 caatatgctc taaaagttga ctaatggttc ctctttgata ctcttcatag agtttcgtca
    12421 attcgcgtac taaaacaact tggcgatctc cgtaaatctc tttcatgtgt tttagcgtat
    12481 cagagactcg aaacggtgac tcataaaaga tttgtgtttc agggtaatct tgctttgttt
    12541 caaagaaagt tatttgttga cctttcttac gtggtaagaa gccataaaaa atatgaggtt
    12601 gtggagctaa acctgaagcg atgagagcag taataccagc gctagctcct ggtatagata
    12661 caactgggat atccccttca atagcagcct tgacaaggtc atgtcctggg tcagaaatag
    12721 agggcattcc tgcatcagat acttgggcta aagatttccc ttcttttaac aaatcaatta
    12781 acccagagat tttatcgtaa gcattgtgtt cgtgaaaact aatttgttta gtagtaatat
    12841 caaagtgctt gagtaaaagt cccgtatttc gtgtatcctc tgcacaaata aaatcaactt
    12901 ctcttaaaat cctaatggca cgaaaagtca tatcatctag attaccaatt ggagttggga
    12961 ctagatagag tgttccgtaa tgtatatttg atttaaaact tttttgaact tgcatttcca
    13021 tcaatctcta tataataatt caatacaaaa agcacattct tcgtcatttt cacgacgttg
    13081 accatagaaa gtattacaaa tgtgaaagcc atcattgtaa atgccttcga gtaattctct
    13141 tccttgttta gaatttttaa ctgcagtttc tgtttctgct tcaataacgc tcagacgctc
    13201 acgaagttta ccattttcaa tgcgtagaac agtattctcc tccaataatt tttggatttg
    13261 ttttttcata gtctcaattt cagacaaccc aactaataaa ttttgtgaaa aatcatcaaa
    13321 ggcatcaaac aagtcttttt tatccattca ctcttcctct ctgcgcgtat tattttctaa
    13381 gaaagcacca tatactctaa actattttga aaactaacat tactcttcca catttgctga
    13441 gctctataag tattttctaa atcataaagt tggccttctt tagccagcat tattgtcagc
    13501 cattgaaaaa caaatgcctg atcattttta tctacaactt gcgatgtcaa gcgagatact
    13561 tctaaataag cagtttgcct atcttttgcc aaagtcgtca caaactgttg acttaaattc
    13621 aagtaatcta ataacttttt gttagtcaac aatatttcta aatgtttagg atcgtctgct
    13681 acttgagcta ataagctagc ttgtgatttt agcaatcctg cttgttcagc ttgatgaacc
    13741 aacatgtcca attgttttgg aaaacgaaaa atttgcgttc tactcttaat tgttggcaaa
    13801 acattgtttt catcattagt caataagatg acataagatg aactttgagg ttcttcaata
    13861 aatttcaaaa gactgttggc tgcattgaca tgcatttttt cgcaatattt gatgataaag
    13921 acctgtgatt tcccttcgaa acctgatctt gaaaaatctt tagtcagctc ttttatagtc
    13981 tctatcttaa taagttgccc ctgtggttct atgattttaa catctgaaaa ttccccatta
    14041 gcgattaaac gacaggctct acactcttga catggtaaac cactctggcg cttttcacag
    14101 aactgacttt gtgctaaata aagtgccata tctagacttg caaaatttcc cgaaaaaaga
    14161 taggcatgac tcatcctatc tgactgtaaa atagtattaa acttttccag caatttgggt
    14221 tgtgttcgct ttaaatccat gacctttgta cttcctgatt cattggttga tacgatctaa
    14281 aataatagag aatgtctcag ctattacttc atctagctgt tgggaggcat caatcgtcac
    14341 aatgcggttt ggctccgaat ccgccaattc aaggtaacct tgacggacgc gttgatgcat
    14401 atctaattgc tcgagatcta gacgatttac ctccctttga acactttttt gaatacgctc
    14461 taaacctact tcagaaggaa catcaaaata taaagttaaa tcaggtttat gactatcagt
    14521 cgcatagtca ttaagccatt ttatatgtga cttatctaat cctcttcctg atccctgata
    14581 ggcaacagaa ctatcaataa aacgatccat taggactatt ttcccttctt ctagagctgg
    14641 gagtaccttt tccactagat gttggcgtcg agcagccatg taaagcaata gctctgtttt
    14701 tttatccatt tgcgtgtgtt taacatctaa aataatatga cgaatttctt ctgaaatagt
    14761 tacgccacca ggctctctgg tggtgagaat atcttgtgat aatttttctc tgagcaaggg
    14821 taaaacagct tctaaaacgg ttgttttacc cgcaccatct gggccctcaa aagaaatcat
    14881 tagtcctttt ttcatggtac atcctttatt tataattact actattctat caaaatttat
    14941 caaaaaaaga cacccacaac ttgattgtgc atgtctgata tttttaa
//
LOCUS       DATRUK010000054        14353 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.054, whole genome shotgun sequence.
ACCESSION   DATRUK010000054 DATRUK010000000
VERSION     DATRUK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14353
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.054"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            17..370
                     /gene="yaaA"
                     /locus_tag="VBE34_001526"
     CDS             17..370
                     /gene="yaaA"
                     /locus_tag="VBE34_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEO7264233.1"
                     /translation="MDYKLFDEYITLQSLLKEIGIIQSGGAIKKFLADNQVLFNGDLE
                     NRRGKKLRLGDIITIPDQNIEIIIRKPSDQEIEERNVEIAEKQRVSAIVKEMNKNTNK
                     DKSKTSKKPVRFPGT"
     gene            373..1482
                     /gene="recF"
                     /locus_tag="VBE34_001527"
     CDS             373..1482
                     /gene="recF"
                     /locus_tag="VBE34_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEO7264234.1"
                     /translation="MWIKNISLKHYRNYEEAQVDFSPNLNIFIGRNAQGKTNFLEAIY
                     FLALTRSHRTRSDKELVHFKHHDVQITGEVIRKSGHLNLDIQLSEKGRITKVNHLKQA
                     KLSDYIGAMTVVLFAPEDLQLVKGAPSLRRKFLDIDIGQIKSTYLAELSNYNHVLKQR
                     NTYLKTTNNVDKTFLTVLDEQLADYGSRVIEHRFDFIQALNDEADKHHYIISTELEHL
                     SIHYKSSIEFTDKSSIREHFLNQLSKSHSRDIFKKNTSIGPHRDDITFFINDINATFG
                     SQGQQRSLILSLKLAEIELIKTVTNDYPILLLDDVMSELDNHRQLKLLEGIKENVQTF
                     ITTTSLEHLSALPDQLKIFNVSDGTISINEKKATD"
     gene            complement(1494..2360)
                     /locus_tag="VBE34_001528"
     CDS             complement(1494..2360)
                     /locus_tag="VBE34_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEO7264235.1"
                     /translation="MEGLLIALIPMFAWGSIGFVSNKIGGRPNQQTFGMTLGALLFAI
                     IVWLFKQPEMTASLWIFGILGGILWSVGQNGQFQAMKYMGVSVANPLSSGAQLVGGSL
                     VGALVFHEWTKPIQFILGLTALTLLVIGFYFSSKRDVSEQALATHQEFSKGFATIAYS
                     TVGYISYAVLFNNIMKFDAMAVILPMAVGMCLGAICFMKFRVNFEAVVVKNMITGLMW
                     GVGNVFMLLAAAKAGLAIAFSFSQLGVIISIIGGILFLGETKTKKEQKWVVMGILCFV
                     MGAILLGIVKSY"
     gene            complement(2416..3078)
                     /locus_tag="VBE34_001529"
     CDS             complement(2416..3078)
                     /locus_tag="VBE34_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7264236.1"
                     /translation="MKNYGKILKELREDKGISLSSLAKSAQLSKSTLSRFENGETQIG
                     IDKFIKALQTLEVGVTINEVSILDSKVKAGTSNTDLEQLTLLESYRDNEDIMRIFSFQ
                     EQQSCDRIESNVLKILAKLFISNLGLNMRLPQDEINLVVTYLNGVTQYNDFYFKVICY
                     FQDILPEDVILNKISNMTKEQLPYSKSLVNLLIKQVIIALEKDSVDKAIVFADLANKV
                     SQ"
     gene            complement(3154..4635)
                     /gene="guaB"
                     /locus_tag="VBE34_001530"
     CDS             complement(3154..4635)
                     /gene="guaB"
                     /locus_tag="VBE34_001530"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEO7264237.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNEVDMNTKLADNLTL
                     NIPIITAAMDTVTDSKMAIAIARAGGLGIIHKNMSIVDQAEEVRKVKRSENGVIIDPF
                     FLTPDNTVSEAEELMQNYRISGVPIVETLENRKLVGIITNRDMRFISDYKQLISEHMT
                     SQNLVTAPIGTDLETAERILHEHRIEKLPLVDDEGRLSGLITIKDIEKVIEFPKAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQITAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIVFQMLGGIRSGMGY
                     VGAANIKELHDNAQFVEMSGAGLKESHPHDVQITNEAPNYSVH"
     gene            complement(4792..5277)
                     /locus_tag="VBE34_001531"
     CDS             complement(4792..5277)
                     /locus_tag="VBE34_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO7264238.1"
                     /translation="MNKKETRHQLIRSLVSETKVRTQHELRELLEKNGVSVTQATLSR
                     DMKELNLIKVNESSDNATETYYEIHSISQKRWEERLRFYMEDALVMLFPVQNQIVLKT
                     LPGLAQSFGSILDSILLPEILATVCGDDTCLIICNNSEEAQKCFEKLSQYTPPFFFSK
                     K"
     gene            complement(5287..5967)
                     /locus_tag="VBE34_001532"
     CDS             complement(5287..5967)
                     /locus_tag="VBE34_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO7264239.1"
                     /translation="MITKEQYFYFRQLDDFKHFTIEQFDHIVSHIKHRTALKNHTLFF
                     EGDYREKLFLIQSGHVKIEQSDASGSFIYTDYVRQDTVFPYGGLFLDDDYHFSAVAIT
                     DIEYFSLPMALYEEYSLQNINQMKHLCRKYSKLLRVHEIRLRNMVTSSASMRVIQSLA
                     TLLLQVPTERGHLPFPITTIEIANMSGTTRETVSHVLKELRQKDIVEMKGKKLLYNNK
                     NYFKKFIE"
     gene            6175..6879
                     /locus_tag="VBE34_001533"
     CDS             6175..6879
                     /locus_tag="VBE34_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEO7264240.1"
                     /translation="MTKFIVDSSYWNLFPTSKIGVILIKDYHMDRNLETELKQLLSDS
                     HSLAKKYLQEKEFSQNKVIQTYRKAYQTFKTKKGARSSIEALLKRINSGNEITSINPL
                     VDIYNAASLRFGLPIGAEDSDTFRGDLKLTITNGGDEFYLIGEDFNIPTLSGELAYVD
                     DVGAVCRCFNWRDGKRTMITDNTQNAFLVIELIDNRREIIFKEALDFIATNTNRFLKA
                     KTQTIILDKEHSEITL"
     gene            7145..8377
                     /gene="arcA"
                     /locus_tag="VBE34_001534"
     CDS             7145..8377
                     /gene="arcA"
                     /locus_tag="VBE34_001534"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEO7264241.1"
                     /translation="MTQTHPIHVFSEIGKLKKVMLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRNEGVEVLYLENLAAESLTNQEIREQFIDEYIGEANVRGRA
                     TKKAIRELLLNIKDNKELIEKTMAGIQKSELPEIPSSEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGNGVSLNHMFSETRNRETLYGKYIFTHHPEYGGKVPMVYDREETTRI
                     EGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHLD
                     TVFTMVDYDKFTIHPEIEGDLRVYSVTYENQDLHIEEEKGDLADLLAKNLGVEKVELI
                     RCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKINGSELV
                     RGRGGPRCMSMPFEREDL"
     gene            8383..8813
                     /locus_tag="VBE34_001535"
                     /pseudo
     CDS             8383..8813
                     /locus_tag="VBE34_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772116.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8829..9842
                     /gene="argF"
                     /locus_tag="VBE34_001536"
     CDS             8829..9842
                     /gene="argF"
                     /locus_tag="VBE34_001536"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO7264242.1"
                     /translation="MTQVFQGRSFLAEKDFSREEFEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTIKENFGKLEGITLVY
                     CGDGRNNVANSLLVAGTLMGVNVHIFSPKELFPAEEIVKLAEGYAKESGAHVLVTDNV
                     DEAVKGADVFYTDVWVSMGEEDKFKERVELLQPYQVNMELIKKANNDNLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            9905..11332
                     /gene="arcD"
                     /locus_tag="VBE34_001537"
     CDS             9905..11332
                     /gene="arcD"
                     /locus_tag="VBE34_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEO7264243.1"
                     /translation="MEKEKKLGLLPLTMLVIGSLIGGGIFDLMQNMSSRAGLVPMLIA
                     WVITAIGMGTFVLSFQNLSEKRPDLTAGIFSYAKEGFGNFMGFNSAWGYWLSAWLGNV
                     AYAALLFSSLGYFFKFFGNGNNIISIIGASIVIWVVHFLILRGVNTAAFINTVVTFAK
                     LVPVIIFLISALLAFKFNIFSLDIWGNGLHQSIFNQVNSTMKTAVWVFIGIEGAVVFS
                     GRAKKHSDIGKASILALFTMISLYVLISVLSLGIMSRPELANLKTPAMAYVLEKAVGH
                     WGAILVNLGVIISVFGAILAWTLFAAELPYQAAKEGAFPKFFAKENKNKAPINSLLVT
                     NLCVQAFLITFLFTQSAYRFGFALASSAILIPYAFTALYQLQFTLREDKSTPGHQKNL
                     IIGILATIYAVYLIYAGGFDYLLLTMIAYTLGMILYIKMRKDDKLPIFVRYEKISAIV
                     ILALCLLCIIEIMTGQIDIAAILAG"
     gene            11353..12309
                     /gene="arcC"
                     /locus_tag="VBE34_001538"
     CDS             11353..12309
                     /gene="arcC"
                     /locus_tag="VBE34_001538"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO7264244.1"
                     /translation="MVKEHQKIVVALGGNAILSTDASAKAQQEALINTSKSLVKLIKE
                     GHDVIVTHGNGPQVGNLLLQQADSDSEKNPAMPLDTCVAMTEGSIGFWLQNALNNELQ
                     EQGIDKEVATVVTQVIVDEKDQAFTNPTKPIGPFLSEEDAKKQAQETGSKFKEDAGRG
                     WRKVVPSPKPVGIKEASVIKRLVDSGVVVISAGGGGVPVIEDANTKALKGVEAVIDKD
                     FASQTLSELVDADLFIVLTGVDNVFVNFNKPNQEKLEEVTVSQMKQYITENQFAPGSM
                     LPKVEAAIAFVENKPEARAIITSLENIDNVLAENAGTQIVVN"
     gene            complement(12429..13454)
                     /gene="trpS"
                     /locus_tag="VBE34_001539"
     CDS             complement(12429..13454)
                     /gene="trpS"
                     /locus_tag="VBE34_001539"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEO7264245.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQNEGSYTLFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTLFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPAGFLVYPVAQAADITAFKANLVPVGTDQKPMIE
                     QTREIVRSFNHAYNCQVLVEPEGIYPENDAAGRLPGLDGNAKMSKSLNNGIFLADDMD
                     TVKKKVMSMYTDPNHIKVEEPGQIEGNMVFHYLDVFGRDEDQKEITAMKEHYQKGGLG
                     DVKTKRYLLDILERELSPIRERRLEYAKDMGQVYQMLQKGSEKAQAVAASTLDEVKSA
                     MGLNYFK"
     gene            complement(13562..14254)
                     /locus_tag="VBE34_001540"
     CDS             complement(13562..14254)
                     /locus_tag="VBE34_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO7264246.1"
                     /translation="MRRDDIIMPQDGRKMAVLLGFIGGFIDVYSHMQFHSLVATQTGN
                     IILMVANLGQNDFHFFITKLFSIFFFSIGFLVGIVIKERAVTAFWRLYALCPLLLSTM
                     FIPFVENVNHNIWVALLAFSTGILMLTFSGSRIESEPYTILMTSGNYRKMLQFWYEYI
                     VNRQRTAMQKRRAINYSLVVLAFIIGALVAAIVYDIFAYRAILGVTITLLIIMIHYTI
                     EIIKNDLTLHNV"
BASE COUNT         4819 a         2614 c         2365 g         4555 t
ORIGIN      
        1 tggagattaa aaagtcatgg attataaatt atttgatgaa tatattactt tacaatcact
       61 tctaaaagag attggtatca ttcaaagtgg cggagccatt aaaaaatttt tagcggataa
      121 tcaagtattg tttaacggtg accttgaaaa taggcgtgga aagaaactcc gcctggggga
      181 tattattact attcctgatc aaaatatcga gatcattata aggaagccat cagatcaaga
      241 aattgaagaa cgtaatgtag aaatagctga aaaacagcgt gtttcagcaa tcgttaaaga
      301 aatgaataaa aacaccaaca aagataaatc aaaaactagc aaaaaaccag ttcgctttcc
      361 agggacataa aaatgtggat aaaaaatatt tctttaaaac attatcgtaa ttatgaagaa
      421 gcacaagtag atttttcacc caacctcaat attttcattg gccgcaatgc tcaaggaaag
      481 acaaattttt tagaagcaat ctacttcttg gctttgactc gaagtcaccg tacccgttct
      541 gataaagaac ttgtacattt taaacaccat gatgtacaaa ttacaggtga ggtaatccgc
      601 aaaagtggcc atcttaactt agatattcaa ttatctgaaa aaggaagaat cactaaagtt
      661 aaccatctta aacaggcaaa attatctgat tatattgggg ctatgactgt cgtacttttt
      721 gcccctgaag atcttcaatt agttaaaggt gcacctagtc taagacgaaa atttttagat
      781 atagatattg gacaaattaa gtcaacgtac ttagctgaac tttcaaatta caatcatgta
      841 ttaaaacaaa gaaacactta tctaaaaact actaataacg tcgacaaaac ttttttaaca
      901 gtccttgatg aacaattagc tgattatgga agtcgtgtca ttgaacaccg ttttgatttt
      961 atccaagccc taaatgatga agcggacaaa catcactaca ttatcagtac tgagttagaa
     1021 catttatcta ttcactacaa atcgtcaata gagtttacag ataagtcaag tattagggaa
     1081 cattttttaa accaattatc taaaagccac tcaagagaca tctttaagaa aaacacaagc
     1141 atcggacctc atcgtgatga tattactttc tttattaatg atatcaatgc tacttttggt
     1201 agccaagggc aacaaagaag ccttatttta tcattgaaat tagcagaaat tgagctaata
     1261 aaaaccgtta ccaacgatta tccgattctc ttacttgacg acgtcatgag tgaacttgac
     1321 aaccatcgtc aactaaaact cttagaggga atcaaagaga atgttcaaac atttattact
     1381 acaacaagtt tagaacattt aagtgcttta cctgatcaac tcaaaatctt taacgttagt
     1441 gatggtacta ttagtattaa tgaaaaaaaa gcaacagatt aatctgttgc tttttaataa
     1501 gatttaacaa taccaagtaa tatagcaccc ataacaaaac aaaggatacc catgacaacc
     1561 catttctgct ctttcttcgt ttttgtctca cctaaaaata aaataccacc aataatagag
     1621 ataattactc caagttgaga aaaactaaaa gcaattgcta gccctgcttt agctgctgcc
     1681 aataacatga agacattacc aacgccccac atgagacctg taatcatatt tttaacaaca
     1741 acagcctcaa agttaacacg aaacttcatg aaacaaattg cacctagaca cattccaaca
     1801 gccatgggta aaatgacggc catagcgtcg aacttcataa tgttgttaaa taaaactgcg
     1861 tacgagatgt aacctacagt tgaataagca attgtagcaa atccttttga aaactcttga
     1921 tgtgttgcca aagcttgttc tgaaacatca cgtttacttg agaaatagaa gccgataact
     1981 aataatgtca acgctgtcaa tcctaaaata aattggattg gcttagtcca ttcatgaaag
     2041 actaaagcac caactaggct tccacctact aattgtgcac cacttgacag tggattagca
     2101 acagagactc ccatatattt cattgcttga aattgaccat tttggccgac tgaccatagg
     2161 ataccaccta agataccaaa aatccacaat gaggcagtca tctctggctg tttaaataac
     2221 catacgataa tcgcaaatag caatgctcct aaagtcattc caaatgtttg ttgatttgga
     2281 cgccctccaa ttttattact aacaaatcca atacttcccc acgcaaacat gggaatcaat
     2341 gcaataagta aaccttccaa ttctttctcc ttacaatgga atattaagta ttacctattt
     2401 tcaatcctag agaatctatt gactaacttt attagcaaga tcagcaaata ctatagcttt
     2461 atcaacacta tctttttcca aagctataat gacttgtttt atcaataaat tgaccagtga
     2521 tttagaataa ggtaactgtt ctttagtcat attactaatc ttatttaaaa taacatcttc
     2581 aggtaaaata tcttgaaaat agcagatgac tttgaaataa aaatcattgt actgtgtcac
     2641 accattcaga taagtaacta ccaggttaat ctcatcttga ggcagcctca tatttaaccc
     2701 taaatttgaa ataaaaagtt tagccaatat tttgagaaca ttgctttcta ttctatcaca
     2761 ggattgttgt tcttggaaac tgaaaattct catgatatct tcattatctc gataagattc
     2821 cagtaaagtt aattgctcta aatcagtatt agaagttcca gccttaactt ttgaatctag
     2881 aatggatact tcattgatag ttaccccaac ctccaacgtt tgtaaagctt taataaattt
     2941 atcaatacct atttgtgtct caccattttc aaaacgtgat agagttgatt ttgataattg
     3001 agcacttttg gctagagaac ttagggatat acctttgtct tctctcaact cttttaaaat
     3061 ttttccataa tttttcatac aaaaacttta ctatatttgt ttcttttatg caacaaaaag
     3121 catcctcata ggggatgctt ttttgtcctt attttagtgt actgaataat ttggtgcctc
     3181 atttgtaatt tgaacatcat ggggatggct ttctttcaaa ccagctcctg acatttcaac
     3241 aaattgagca ttatcatgaa gttcttttat gttcgctgca cctacatatc ccataccaga
     3301 acgaatgcca ccaagcattt ggaaaacaat atcggctact gaacctttgt atgctacacg
     3361 accttcaata ccttctggaa ccaatttgtt agcctcgttt acagaacctt ggaaataacg
     3421 atcgcttgaa ccttttttca ttgctgcaat tgagcccata ccacggtatg tcttgaactt
     3481 acgcccttgg aaaatttctg tttctcctgg cgcttcatct gttcctgcaa acattgaacc
     3541 tagcataact gcatttccac ctgctgcaag tgcttttaca atatcacctg aatatttaat
     3601 accaccatca gcaatgatag tcttaccgta ctcacgcgca acagccgctg catcgtaaat
     3661 agctgtaatc tgaggaacac ctaccccagc aacgacacga gtcgtacaaa ttgaacctgg
     3721 accaatacca actttaacga catccacacc tgcatcataa agtgcacgcg ctccttctgc
     3781 tgtagcaata tttcccgcaa tcaatgtgcg attagggaag tgagcacgaa tttcagcaat
     3841 tttacggagc acaccagctg aatgtccatg tgctgtatca atgacaatag catctgcacc
     3901 tgcttcaaac aaagcttctg cacgttcaaa agtatcagag gttacaccta ctgcacctgc
     3961 aactaagaga cgtccaaatt catcttttgc tgccttagga aattcaataa ctttttcgat
     4021 atccttaata gtaataagtc ctgaaagacg tccctcatca tctactaaag gtagcttttc
     4081 aatacgatgc tcatgaagaa tacgctccgc agtttcaaga tctgtaccaa taggagctgt
     4141 tacaagattt tgacttgtca tgtgctctga gattagttgt ttataatcag aaatgaaacg
     4201 catatcacgg ttagtaataa ttccaactaa tttacgattc tcgagtgtct ctacaatagg
     4261 aactccacta atgcgatagt tttgcatcaa ttcctcagct tcagagacag tattatctgg
     4321 tgtcaagaag aatgggtcaa taataacacc attttctgag cgtttaactt tacgaacttc
     4381 ttctgcttga tcaactattg acatattttt atgaatgata ccaagcccac cagcacgtgc
     4441 aatagcaata gccatcttac tatctgtaac tgtatccatt gcagctgtaa tgattggaat
     4501 gtttaatgtc aaattatcag ccaactttgt attcatatca acttcatttg gtaaaacatg
     4561 gctttcagca ggaataagca gaacatcatc aaaagtaaaa ccttttttca aaaacttagt
     4621 atcccaattt gacatcaaaa aatcctcttt tcttagatag tagatataaa aaatcccatt
     4681 aaaattatat tgttactatc atatcacgct aaaatcattt gtcaatataa aaagagcata
     4741 gaattaatag cttctagctc tttaaagtaa tgaaaaaatc ttcttaattt tctatttttt
     4801 tgagaagaag aaaggcggcg tatactgact taatttctca aaacactttt gagcttcttc
     4861 actattatta caaataatca aacaagtatc atcaccacag acagttgcta agatctcagg
     4921 taataaaatc gaatctaata tggagccaaa agattgagct aagccaggta gtgtcttcaa
     4981 aacgatttgg ttttgaactg gaaacagcat gactagggca tcttccatgt aaaaacgtaa
     5041 ccgttcttcc caacgcttct gagatatact atgaatctca taataagttt ctgtagcatt
     5101 atcactgctt tcgttaacct taattaaatt aagttccttc atatctcgag ataatgttgc
     5161 ttgcgtcact gaaacgccat ttttttctag taattccctt aactcatgtt gtgtacgcac
     5221 cttagtctca gagactaagg aacgaattaa ttggtgtctt gtttcttttt tattcatata
     5281 aactccttat tcaatgaatt tcttgaaata atttttatta ttatagagaa gcttctttcc
     5341 tttcatttca acgatatctt tttggcgtaa ttcctttaaa acatgactaa ctgtctcgcg
     5401 agtagttccg ctcatattgg caatttcaat cgttgtaata ggaaaaggta aatgtcccct
     5461 ttctgtagga acttgcaaaa gtagagttgc aagcgattgg ataactctca tactggcact
     5521 tgaagtcacc atattacgaa gtcgtatttc atgaactcgt aatagcttag aatatttccg
     5581 acaaagatgc ttcatttgat tgatattttg aagagagtat tcttcgtata aagccattgg
     5641 cagagaaaaa tactcaatat ccgtaatagc tactgctgaa aaatggtaat catcatccaa
     5701 aaagagacct ccataaggaa aaactgtatc ttgtctaaca taatctgtat aaataaaaga
     5761 accagaagcg tctgattgtt caatttttac atgtcctgac tggattaaaa acagtttttc
     5821 acggtaatca ccttcaaaaa ataaggtatg attttttaga gctgtacgat gtttaatgtg
     5881 gctcacaata tggtcaaatt gttcaatagt aaagtgttta aaatcatcta gttgtctgaa
     5941 ataaaaatat tgttcttttg taatcacctg attttctaga cctttctaat cattagatgc
     6001 gaacattttt tgtataatga aaatttttta atgtataaaa ttttcttgtc acgataaatc
     6061 ctccttcatt atagcaaaaa aattcgtaga aagaacgttt ttcgttgaat tttgaaaacg
     6121 cttttgagaa aatgtaattg ttctgatatc ttttaaaaaa attaagaggc cttcatgact
     6181 aaatttattg ttgattcatc gtactggaat ttgtttccaa cttctaaaat tggagtaatc
     6241 ttaatcaaag attatcacat ggacaggaac cttgaaacag aacttaaaca gttactgtct
     6301 gatagtcatt ctttagcaaa aaaatatttg caagagaaag aattcagtca aaacaaagtt
     6361 atccaaactt atcgcaaagc atatcaaacc tttaaaacta aaaaaggtgc acgttcaagt
     6421 atcgaagcat tattgaagcg aattaattca ggaaacgaaa ttactagtat aaatcctcta
     6481 gttgatatct ataatgccgc tagccttagg tttggattac ctattggtgc tgaggatagt
     6541 gatacattta gaggtgattt aaaactaact attaccaatg gtggtgatga attttacttg
     6601 attggcgaag attttaacat accaactctt tcaggtgagc ttgcctatgt tgatgatgtt
     6661 ggagcagttt gccgctgttt taattggcgt gatggaaaac gtactatgat taccgataat
     6721 actcagaacg cttttttagt tatcgaatta attgataata gaagagaaat tatttttaaa
     6781 gaagcgttgg actttatcgc caccaataca aatcgctttc taaaagctaa aacacagacc
     6841 atcatattag ataaagaaca cagtgaaata acgctataac attaaaacga aaagtaaaaa
     6901 ttttactttt cgttattttt taggttaata atttctcact ttccaacgat aaaagaaaga
     6961 aattgtgtag aaatcgcttt catttttata aatttaaatt tttttggata taatcgtgac
     7021 attcttaaca cttttaatgc gaataacttc cctattttat attcaaaaga aagtagaatg
     7081 taagtgtaga tatagagata aatcctgttt caatgcaaat ctctaactaa gaaaggagta
     7141 aaatatgact caaacacatc caattcatgt tttctcagaa attggaaaac ttaaaaaagt
     7201 tatgctacac cgtccaggta aagaaataga aaatctgatg cctgattatt tagagagact
     7261 cttgtttgat gatatcccat ttttggaaga tgcacaaaaa gaacacgatg cgtttgcaca
     7321 agcacttcgc aacgaaggag tcgaagtact ttaccttgaa aatcttgctg ccgagtcgct
     7381 aacaaaccaa gaaattcgtg aacagtttat cgatgagtac attggcgaag caaatgttcg
     7441 cggtcgtgct actaaaaaag ctattcgaga attattactc aatatcaaag ataataaaga
     7501 acttatcgaa aaaactatgg caggtatcca aaagtctgag ctacctgaaa tcccatcttc
     7561 tgaaaaaggt ctaactgatc ttgtcgaatc taattatcca tttgccatag atccgatgcc
     7621 aaatctttac tttacacgtg atccatttgc aactattggg aatggagttt cattaaacca
     7681 tatgttctct gaaacacgta atcgcgaaac tttatacggt aaatatatct ttacacatca
     7741 cccagagtac ggtggaaaag tccctatggt ctatgatcgt gaagaaacaa ctcgtattga
     7801 aggtggagat gagctcgtcc tttctaaaga tgtccttgcc gtgggtatct ctcaacgtac
     7861 tgatgctgcc tctattgaaa aactcttagt caatattttt aaacaaaatt taggttttaa
     7921 aaaggtttta gcatttgaat ttgcaaataa tcgtaaattt atgcaccttg acacagtctt
     7981 tacaatggtt gactatgaca agtttacaat tcacccagaa attgaaggcg atcttcgcgt
     8041 ttactctgta acttacgaaa accaagatct tcatattgaa gaagaaaaag gtgatttggc
     8101 agatcttcta gcgaaaaacc taggagttga gaaagtggaa ttgattcgtt gcgggggtga
     8161 caacttagtc gctgctggac gtgaacaatg gaatgatggt tcaaatacat taactatagc
     8221 accaggtgtc gttgtcgttt acaacagaaa tacaatcaca aatgcaatct tagagtccaa
     8281 aggattgaaa ttaattaaga ttaatggaag tgaattagtt cgtgggcgtg gtggaccacg
     8341 ctgcatgtca atgccatttg aacgtgaaga cctttaaaat ttatgaagct attagtcaat
     8401 cacttgcctt accaaagagc tgcaagccta tatattcgct tttctgtttt cgttatcgaa
     8461 agaaatataa aaatgaaaga agaattcgat gataatgacg agcaagatac tatttatgct
     8521 gttctttatg atggaaacag ccagtttcaa caggacgctt tcttcctgaa actcaaacag
     8581 aagctcgttt gactcgaatt gcgactttaa aaggttatcg tggtaacggc tatggtacca
     8641 aaattattat agcactagaa aattatgcaa aagaaaatgg ttatcactat ctaactatac
     8701 atgctgaatt gacagcaaaa gatttctacc agacacttgg gtatcaagct actgggaata
     8761 tttatatgga agacggggaa gcctgtcaaa cactcgaaaa atatttaatt taaaggagac
     8821 attaacatat gacacaagta tttcaaggac gtagtttctt agcagaaaaa gatttttctc
     8881 gtgaggaatt tgaatatctt attgattttt cagctcattt aaaagacctt aaaaaacgtg
     8941 gtgttcctca tcattatctt gaaggtaaaa atattgctct cttatttgaa aaaacatcta
     9001 ctcgtactcg cgcagccttt acaactgcag caattgacct aggcgctcat ccggaatacc
     9061 ttggtgcaaa tgatattcaa cttggtaaaa aagaatcaac agaagatact gctaaggttt
     9121 taggacgtat gtttgatggt attgaattcc gtggttttag ccaaagaatg gttgaagagc
     9181 ttgctgaatt ttctggagta cctgtctgga atggtttaac agatgaatgg catccaacac
     9241 aaatgctagc tgactacctt actatcaaag aaaacttcgg taaacttgaa ggtattactc
     9301 ttgtttactg tggtgacgga cgtaacaatg ttgccaactc gcttttagtg gctgggactt
     9361 tgatgggggt caatgtacac atcttttctc caaaagaact tttccctgct gaagagattg
     9421 ttaaattggc tgaaggatat gccaaagaat ctggggctca cgttctcgtt actgataatg
     9481 tagacgaagc tgtaaaggga gcagacgtct tttacactga tgtctgggta tcgatgggag
     9541 aagaagataa gttcaaagaa cgcgttgaac ttcttcaacc atatcaagta aacatggaac
     9601 tgattaaaaa agctaataat gataatctta tcttcttaca ctgcttacct gcattccatg
     9661 atacaaatac cgtttatggc aaagacgtcg ctgaaaaatt tggggtcaag gaaatggaag
     9721 ttactgatga agtcttccgt agcaaatatg ctcgtcattt cgaccaagct gaaaatcgta
     9781 tgcacactat taaagctgta atggctgcaa cccttggaaa tcttttcatt ccaaaagttt
     9841 aaattaaacg actataggga gttgacatat agtcaattcc ctattttagg aaatgaggta
     9901 taaaatggaa aaagaaaaaa aattaggtct tttaccacta acaatgcttg tcattggctc
     9961 tcttatcggt ggcggaatct ttgatttaat gcaaaatatg agttccagag ccggtttggt
    10021 accaatgctt attgcttggg taattactgc tatcgggatg ggaactttcg ttttaagttt
    10081 tcaaaattta tctgaaaaaa ggccggacct aacagctgga atctttagtt acgctaaaga
    10141 ggggtttgga aactttatgg gatttaactc tgcatggggt tattggttat cagcttggct
    10201 tggaaatgtt gcctacgctg cactcttatt cagttcactc ggttatttct ttaaattctt
    10261 tggtaatgga aataatatca tctcaattat tggagcaagt atagttattt gggttgtcca
    10321 tttcttaatt ttaagaggtg ttaatacagc tgcatttatt aataccgtag ttacctttgc
    10381 aaaattagta cctgttatta ttttcttaat ttcagcgtta ttagctttca aatttaacat
    10441 ttttagtctt gatatctggg gaaatggatt acatcaatca attttcaacc aagtcaattc
    10501 aactatgaaa accgctgttt gggtatttat tggtattgag ggcgccgttg tcttctcagg
    10561 tcgtgctaaa aaacactctg atattggtaa agcaagtatc ctagcattat tcactatgat
    10621 ttcactttat gtattgattt ctgttttatc acttggtatc atgtcacgtc cagaacttgc
    10681 aaacttaaaa acaccagcta tggcttacgt tctagaaaaa gctgttggtc actggggtgc
    10741 tatcttagtt aaccttggtg ttatcatttc agtatttggc gctattcttg cttggacttt
    10801 atttgcagca gaattaccat atcaagctgc taaagaaggt gcttttccta aattttttgc
    10861 aaaagaaaat aaaaacaaag ctccaatcaa ctcactctta gtcactaatc tttgtgtaca
    10921 agcattctta atcacgttct tattcacaca aagtgcttat cgttttggtt tcgcattagc
    10981 atcatctgct atcttaattc cttatgcttt tacagcacta tatcaattac aattcacact
    11041 ccgtgaggat aagtcaactc caggacatca aaagaattta attatcggta tcctcgctac
    11101 aatctatgct gtttacctta tctacgctgg tggttttgat tacttacttt tgacaatgat
    11161 tgcttatact ctaggtatga ttctctatat taaaatgaga aaagatgaca agcttcctat
    11221 ctttgtgaga tatgaaaaaa ttagtgccat tgtcattcta gcgctttgct tactatgtat
    11281 tattgaaatt atgactggac aaattgacat tgctgcaatc ctagcaggtt agtcattaga
    11341 aagagggata aattggtaaa agaacatcaa aaaatcgtag ttgccttagg tggtaacgct
    11401 attctatcaa cagatgcttc agcaaaagca caacaagaag cattaatcaa cacatcaaaa
    11461 tcactggtca aactgattaa agaaggccat gatgtcattg ttactcatgg aaatggtccc
    11521 caagttggta acttattgct acagcaagca gattcagatt ctgaaaagaa tccagcaatg
    11581 cctttggata cttgtgttgc catgacagaa ggaagtattg gtttttggtt acaaaatgct
    11641 cttaacaatg aattacaaga acaaggcatt gacaaagagg ttgcaacagt ggtaactcaa
    11701 gttattgtag atgaaaaaga tcaagctttc actaatccaa caaaacctat tggcccattc
    11761 ttatctgaag aagatgccaa gaaacaagcc caagaaacag gttcaaaatt taaagaagat
    11821 gctggtcgtg gatggcgtaa agtcgttcct tcaccaaaac cggttggtat caaagaagct
    11881 tctgttatta aacggttagt ggactctggt gtggttgtta ttagcgcagg tggcggaggt
    11941 gtaccagtta ttgaagatgc taatacaaaa gcattaaaag gggttgaagc tgttattgac
    12001 aaagactttg ccagtcaaac tttatcagaa cttgttgatg ccgacttatt catcgtctta
    12061 actggcgttg acaacgtttt tgttaatttt aataaaccta atcaagaaaa attagaggaa
    12121 gttactgttt cacaaatgaa acaatatatc actgaaaatc aatttgcccc tggcagtatg
    12181 ctaccaaagg tagaagctgc gattgctttt gttgaaaata aaccagaagc gcgtgctatc
    12241 attacttcac ttgaaaatat tgataatgtc ttagcagaaa atgcagggac acaaattgtt
    12301 gtaaattaaa caatgtagat taccattaca tacactcctt attaataaca aagtaatgtc
    12361 actccttagg cattcctata ataaaagaga gctagatata tctagctctc ttttattata
    12421 ggaatatatt atttaaaata gttcaaaccc atggctgatt taacctcatc caaagtagag
    12481 gctgcaacag cttgagcttt ctcactacct ttttgtaaca tttgataaac ttgtcccata
    12541 tctttagcat attcaaggcg acgttctcta ataggtgata attcacgctc taagatatca
    12601 agtagataac gttttgtttt aacatctcca agtccacctt tttgatagtg ttctttcata
    12661 gcagtaatct ctttttggtc ttcatctctt ccaaaaacat caaggtaatg gaataccata
    12721 ttaccttcga tttgtcctgg ttcttctact ttaatatggt ttggatcagt atacatgctc
    12781 ataacttttt tcttaactgt atccatatca tctgctaaaa agattccatt atttaatgac
    12841 ttagacattt ttgcattacc atctaaccct ggcagacgac ctgctgcatc attttctgga
    12901 tagattccct ctggttcaac taaaacctga caattataag catgattgaa agatctaact
    12961 atttcacgtg tttgttcaat cattggtttt tggtcagtcc ctacaggaac aaggttagct
    13021 ttaaaagcgg ttatatcagc tgcttgtgca acaggataaa ctaagaatcc tgctggaata
    13081 gactcaccaa aacctttttg agcaatttct gtttttacag ttggattacg ttccaaacgt
    13141 gcaagtgaaa ccaagttcat ataatacata cttaattctg ctaactcagg aatttgactt
    13201 tgaatgaata aagtcgactt gtttggatcc agcccaacag ctaaataatc aagtgcaaca
    13261 ttaccaattg attcaacaat agtttgcgga tctttagcat ggtctgtcag tgcttgttgg
    13321 tctgctaaaa agacaaataa agtatagcta ccctcatttt gaagtagaac tctatttttt
    13381 aagctaccta cataatgtcc aatatgcaac tttcctgtcg gtctatcacc tgttaagata
    13441 attggttttg tcatgtttac tccttaatat accaaagata tcatttcata tagttatctt
    13501 atcatgtcac aagccctatt gcaattcatc cctacaagaa acattcacct tattaagatg
    13561 attaaacatt atgtaacgtt aaatcgttct taataatttc aatcgtataa tgaatcataa
    13621 taattaaaag cgttatcgtc acacctaaaa tagctctata tgcaaagata tcgtaaacta
    13681 ttgcagctac caatgctcca ataataaatg ctagaactac cagtgaataa ttgatagctc
    13741 gtcttttttg catagccgtt ctctgacgat taacaatata ctcataccaa aattgtagca
    13801 ttttccgata attaccagaa gtcattaaga tagtgtatgg ttcagattca atgcgacttc
    13861 ctgaaaatgt caacattaaa atacctgtgc taaaggctaa taaagctacc cagatattgt
    13921 ggtttacatt ttcaacaaaa ggaataaaca tcgtagaaag taaaagggga cataatgcat
    13981 atagacgcca aaaagctgtt actgcacgtt ctttaatcac aataccaacc aaaaaaccaa
    14041 ttgaaaaaaa gaaaatagaa aataatttag tgataaaaaa gtgaaaatca ttttgtccga
    14101 gatttgccac catcaagata atattacctg tctgtgttgc aacaagggag tgaaactgca
    14161 tgtgactata aacgtcaata aaaccaccaa taaaacccaa taatacagcc atttttcttc
    14221 catcttgtgg cattataata tcatctctgc gcattatagc aaattctcct tcttgtatta
    14281 tcatgttttc tattatatac caataaaata ggcattcaac tcaaaaatat cgagaaattt
    14341 cttttctaaa ttt
//
LOCUS       DATRUK010000055        14223 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.055, whole genome shotgun sequence.
ACCESSION   DATRUK010000055 DATRUK010000000
VERSION     DATRUK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14223
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.055"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..735)
                     /locus_tag="VBE34_001541"
     CDS             complement(<1..735)
                     /locus_tag="VBE34_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7264247.1"
                     /translation="MVKTAVLMATYNGQGFIHDQLDSIRNQTLRPDYVLMRDDGSTDD
                     TVKVVEDYIKEHRLDGWSITSNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDTWYH
                     HKNKMQVDIMEERQDINLLSADIDIKKLSEEATIPNNFKFNGEETISKYPLDFSYHNY
                     RQGWTFCLRKSFVDQVMTFYSDNLILSHDNLFAGVSGVIGSGYNLNRSVGEHKRHAEN
                     ASGNILNMSSSYERHLQELYFVLSYYI"
     gene            complement(744..1679)
                     /locus_tag="VBE34_001542"
     CDS             complement(744..1679)
                     /locus_tag="VBE34_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7264248.1"
                     /translation="MVKTAVLMATYNGEKFISEQLDSIRQQTLKPDYVLLRDDCSTDE
                     TVNVVNNYIAKHELEGWKIVKNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDIWYL
                     DKNERQFAIMSDNPQIEVLSADVDIKTMSTEASVPHFLTFSSSDRISQYPKVYDYQTF
                     RPGWTIAMKRDFAQAIADNWTFGANISHDNLIETTASIIGNGYNLNESVGVHIRYGGN
                     ASGNSKFVLNWKSPKTQHITGLKTFVDFYETMQKVVNKYQNDVALNQRLAFEKERLEA
                     SKLSLIHNSAFVIKNWRLYNSNSGRMRDLLFAFKK"
     gene            complement(1681..2739)
                     /locus_tag="VBE34_001543"
     CDS             complement(1681..2739)
                     /locus_tag="VBE34_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEO7264249.1"
                     /translation="MMIKFSNKIVQEDLALISNQNYINWERIANKGVLISGSNSMLAS
                     YMVFLLAYLNETRNYQTQIIATARNIEKARDKFSDLVGKDYFTLIPYDVEERLEYDGK
                     VDYIIHAASNASPTAILSNPVSIIKANTIGTLNLLDFAKEKTIENFLFLSTREVYGTS
                     IKEVIDEEAYGGFDILATRACYPESKRMAETLLQSYYDQYKVPFTIARIAHSFGPGME
                     LGNDGRIMNDLLSNVIDGKDIVLKSSGTAERAFCYLADAVSGLFTILLNGEVGQAYNV
                     ANEDQPIMIKDLAQKLVDLFSDKNISVVFDIPKTMSAGYSKMGRTRLTMAKLEALGWK
                     REVSLESGILKTVQAFEE"
     gene            complement(2739..3461)
                     /locus_tag="VBE34_001544"
     CDS             complement(2739..3461)
                     /locus_tag="VBE34_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEO7264250.1"
                     /translation="MMNIGVIFAGGVGRRMNTKGKPKQFLEVHGKPIIVHTIDIFQNT
                     EAIDAVVVVCVSDWLDYMNNLVERFNLTKVKAVVAGGETGQMSIFKGLEAAEQLATDD
                     AVVLIHDGVRPLINEEVINANIQSVKETGSAVTSVRAKETVVLVNDSSKISEVVDRTR
                     SFIAKAPQSFYLSDILSVERDAISKGITDAIDSSTLMGMYNRELTIVEGPYENIKITT
                     PDDFYMFKALYDARENEQIYGM"
     gene            complement(3461..4285)
                     /locus_tag="VBE34_001545"
     CDS             complement(3461..4285)
                     /locus_tag="VBE34_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEO7264251.1"
                     /translation="MKEMTVSEIREVQLEMLAYIDKVARDNKIEYSLGGGSLLGAMRH
                     KGFIPWDDDIDLMLERSQYERLMKALADANNSDFKLLHHSVEKNLWPFAKLYHTKSMY
                     LSKTDRIHPWTGIFIDIFPLDRLPESAEERQRFFKKVHSAAANLMCTTYPNFASGSRK
                     LYANARLILGLPRFIAYHGQAKKRAEIVDQVMETYNNQEVPYMGYTDSRYRLKEYFPR
                     EIFSEYEDVMFENIKTRKIKNEHAYLNQLYGGSYMELPPESKRESHSYYTWYWKEG"
     gene            complement(4310..6043)
                     /locus_tag="VBE34_001546"
     CDS             complement(4310..6043)
                     /locus_tag="VBE34_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEO7264252.1"
                     /translation="MSKKHIFICLFFMLFLVGCQNNKKVKTDKKVDITAYYYHNNTPQ
                     YSVQINAIHLQTALDKVRTGEFESKDFTHYTFDSIKRKYQVSGKKVLMDNNYRFGSGI
                     KNTRQDAIAIVRLLLEKNKDYLIYMNGLEEPSVLYNPENKRYKFTNNKGKAIGNIPVG
                     LTAFENSAETQEYVLKNIQKNETIYLGNTRVDNPRVTVNNKKRDTIGVEYGKRITYRI
                     PIYSKQLTVRVSPNFVVDSTNYNYRLSQAPIIAEEGKEGRLYPIDSSISVDGDKIVLN
                     SSGDETEQKELLQNSLYKLHSIDKLDFDLTNADIKELEITGHVASKRDYNLSVAQEKK
                     GVIETKRSLISVDNQKQDQGIFVEDSKGYIKTPSLSSYGINFAMYDDNQNRLLQGSEY
                     ILGRLNADKKVSLYQPNGFWINTQKTIKSLNKEYLNQNAYHIRGNNIIGIDGYSNDID
                     LNPRLWAYNYKKQVKSNQSLFKLRGLSNKYKYFLIQTKSPKGYLQSNKPFYFTVNKDS
                     VSKAQFGNYNINGYIMDMQYNKQEYNALANTPKGQKTKKTFKPMTLVILFAVALFIFY
                     ITAIRFVFKRM"
     gene            complement(6036..6389)
                     /locus_tag="VBE34_001547"
     CDS             complement(6036..6389)
                     /locus_tag="VBE34_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEO7264253.1"
                     /translation="MSLALQIEMFILAIVLLYMIIRMVNKNTFSVKRSLPWLLVGLIL
                     VFISVFPGVVSFIAHHLGFALTMNFLLFGAILFLFCLELFDTSSNTKRDEQIKRLIQE
                     VSLLKKEIEDSTLDE"
     gene            complement(6386..7117)
                     /locus_tag="VBE34_001548"
     CDS             complement(6386..7117)
                     /locus_tag="VBE34_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7264254.1"
                     /translation="MMKKILLVIPAYNEEGSIAKTVQTIVDFKASRSLPFELDYIVIN
                     DGSTDGTPELLDRLGLNHIDLVQNLGIGGCVQTGYLYANRNHYDVAVQFDGDGQHDIR
                     SIEDVVMPILNDEADFVIGSRFVDKKHQNFQSTAMRRLGINLISAAIKLTTGHKVYDT
                     TSGYRAANAALIAYLSCHYPVQYPEPESTARILKKGYRLKEVTANMFEREAGTSSISS
                     LKSIFYMTDVLTSIIIAGFIKEDDK"
     gene            complement(7119..8060)
                     /locus_tag="VBE34_001549"
     CDS             complement(7119..8060)
                     /locus_tag="VBE34_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7264255.1"
                     /translation="MKVNILMATYNGEKFLAQQIESIQKQTFKEWNLLIRDDGSSDKT
                     CDIIRNFTAKDSRIRFINENEHHNLGVIKSFFTLVNYEVADFYFFSDQDDVWLPEKLS
                     VSLEAAKHKASDVPLLVYTDLKVVNQELNILQDSMIRAQSHHANTTLLPELTENTVTG
                     GTMMINHALAEKWFTPNDILMHDWFLALLAASLGEIIYLDLPTQLYRQHDNNVLGART
                     MDKRFKILREGPKSIFTRYWKLIHDSQKQASLIVDKYGDIMTANDLELIKCFIKIDKQ
                     PFMTRLRWLWKYGYSKNQFKHQVVFKWLIATNYYNKR"
     gene            complement(8050..9204)
                     /locus_tag="VBE34_001550"
     CDS             complement(8050..9204)
                     /locus_tag="VBE34_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEO7264256.1"
                     /translation="MQDVFIIGSRGLPAHYGGFETFVSELINHQKSSDIKYHVACLSD
                     KEHHTHFNFADADCFTINPPQLGPARVIAYDIMAINYALDLVKTHDLKEPIFYILGNT
                     IGAFIWHFANKIHKVGGLLYVNPDGLEWKRSKWSRPTQRYLKYAEKCMTKNADLIISD
                     NIGIENYIQSTYSNVKTRFIAYGTEINSRKLSSDDPRVKQLFKKWNIKSKGYYLIVGR
                     FVPENNYETAIREFMASDTKRDLVIICNHQNNPYFEKLSLKTNLQQDKRVKFVGTLYE
                     KDLLDYVRQQAFAYIHGHEVGGTNPGLLEALANTDLNLVLDVDFNKSVAGLSSFYWTK
                     KEGDLAKLINDSDQQQDLSTYGDRAKAIIQENYTWKKIVEEYEDLFLNES"
     gene            complement(9321..10175)
                     /gene="rfbD"
                     /locus_tag="VBE34_001551"
     CDS             complement(9321..10175)
                     /gene="rfbD"
                     /locus_tag="VBE34_001551"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEO7264257.1"
                     /translation="MILITGANGQLGSELRHLLDERTQEYVAVDVAEMDITNAEMVDK
                     VFEEVKPSLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKAAAKHDATLVYISTD
                     YVFDGEKPVGQEWEVDDLPDPKTEYGRTKRMGEELVEKYTSKFYTIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQHGRPTWTRTLAEFMTYLAENQKDFGYYHLSNDAKEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLEKAKATGFVIPTWQDALKE
                     FYKQEVKK"
     gene            complement(10265..10606)
                     /locus_tag="VBE34_001552"
     CDS             complement(10265..10606)
                     /locus_tag="VBE34_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEO7264258.1"
                     /translation="MSEVKQYSEEEVGKIKDRILEALEMVIDPELGIDIVNLGLIYEI
                     RFEDNGRTEIDMTLTTMGCPLADLLTDQIHDVMKTVPEVTETEVKLVWYPAWSVDKMS
                     RYARIALGIRS"
     gene            complement(10715..11824)
                     /gene="rpoD"
                     /locus_tag="VBE34_001553"
     CDS             complement(10715..11824)
                     /gene="rpoD"
                     /locus_tag="VBE34_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019787874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEO7264259.1"
                     /translation="MAEKKGNTTFNVQVADFIRNHKKQGTAIDDEVTEKLVIPFVLDA
                     DQIDDLLERLTDGGISITDKEGNPSTKYVVEGPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELAVAVAEGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLKDFMED"
     gene            complement(11832..13706)
                     /gene="dnaG"
                     /locus_tag="VBE34_001554"
     CDS             complement(11832..13706)
                     /gene="dnaG"
                     /locus_tag="VBE34_001554"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO7264260.1"
                     /translation="MKKLLKKFTQKRKNDIIKCKTIMGYFCGGHDLAIDKEKISEIKN
                     SVNIVDVIGEVVGLTKTGRNHLGLCPFHKEKTPSFNVIEDRQFFHCFGCGRSGDVFKF
                     VEDYQHISFLDSVQVLAERSGIPLDTNFKGQVPKKPKANQSLLDIHRVASGFYHAYLM
                     TTNDGERARQYLAERGVTEDLIKHFQIGLSPGGQDFLYRRLAKEFDEKTLMSSGLFNY
                     SENSNQFYDSFNNRIMFPLTNDIGEVIAFSGRVWTQEDIDRKQAKYKNSRATPIFNKS
                     YELYHLDKARAVINKAHEVYLMEGFMDVIAAYRAGIENVVASMGTALTNEHVRHLKRF
                     TKKVVLTYDGDRAGQNAIDKSLELLSDMTVDIVRIPNKMDPDEFLQANSAEDFKQLLE
                     NGRISNTEFYIHYLKPENTDNLQSEIAYVEKIAKLIAKSPSITAQNSYITKVAELLPD
                     FDYFQVEQSVNNERLHHRSQQQASSSVQTSATVQLPQTGKLSAITKTEMQLFHRLLNH
                     PYLLNEFRNRDNFYFDTTEIQVLYELLKESGEITSYDLSQESDKVNRTYYIILEEQLP
                     VEVSIGEIEAVEKARDRLLKERDLRKQSQLIRQSSNQGDEEGALAALENLIAQKRNME
                     "
     gene            13830..14207
                     /gene="mscL"
                     /locus_tag="VBE34_001555"
     CDS             13830..14207
                     /gene="mscL"
                     /locus_tag="VBE34_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEO7264261.1"
                     /translation="MIKELKEFLFKGNVLDLAVAVILGAAFNAIITSLVKDVITPLIL
                     NPVLKAAGVSNIAQLSWNGVAYGNFLSAVINFLIVGTTLFFIVKAANKVMAKKPAEEE
                     IIEVVEPTQEQLLAEIRDLLANK"
BASE COUNT         4456 a         2806 c         2073 g         4888 t
ORIGIN      
        1 gatatagtaa ctcaaaacaa aatacagttc ttgtaaatgc ctttcgtaac ttgagctcat
       61 attcaagata ttcccacttg cattctcagc atgtcgctta tgttcaccaa cacttctgtt
      121 taaattgtaa cctgaaccaa tgacaccaga tacccctgca aaaagattat catgtgacaa
      181 aattaaatta tcactgtaaa aagtcattac ttgatcaaca aacgacttgc gtaaacaaaa
      241 agtccaacct tgacgatagt tatgatatga aaagtctaat ggatacttag aaattgtttc
      301 ctcaccattg aacttaaaat tattgggaat agtagcctct tctgataact ttttgatatc
      361 aatatcggcg ctaagaaggt ttatatcttg gcgttcttcc ataatatcaa cttgcatctt
      421 atttttatga tgataccatg tgtcatcttg atcactaaaa aagacatagt caacctcata
      481 ggctaacaca tcaataagta attgacgaaa atttaaacgc caacctaaat ttttatcatt
      541 actagttatt gaccaaccat ctaaacggtg ctcttttata tagtcttcaa ccacctttac
      601 agtatcatca gtagagccat cgtccctcat taagacatag tctggtctca acgtttgatt
      661 tctaattgaa tctagctgat cgtggatgaa accttgacca ttatatgttg ccatcaaaac
      721 tgctgttttt accatattat tccttacttt ttaaacgcaa ataataaatc tctcatacgt
      781 ccactattag agttatagag tcgccaattt ttaatcacaa aagcactatt atgaattaat
      841 gacaatttag aagcttccaa tctttctttt tcaaaggcta agcgttgatt taaagcgaca
      901 tcattttgat atttgttgac aaccttctgc atggtttcgt aaaaatcaac aaatgttttt
      961 aatcctgtga tatgttgtgt tttaggcgac ttccaattca aaacaaattt gctattacca
     1021 cttgcgtttc ctccatatcg aatatgaact cctacgcttt cgttaaggtt ataaccattt
     1081 ccaataatac tggcagttgt ctcgataaga ttatcgtgcg aaatattagc tccaaaagtc
     1141 caattatcag cgatagcttg cgcaaaatct ctcttcatag caatggtcca tccgggacgg
     1201 aatgtttgat aatcatatac tttaggatac tgactgattc tatcactaga agaaaaagtt
     1261 agaaaatgtg gaacactggc ttctgtagac atcgttttga tatcaacgtc tgcactcaaa
     1321 acctcaattt gagggttatc tgacataatg gcaaactgtc gttcgttttt atcaagatac
     1381 caaatatcat cttgatcact aaaaaagaca tagtcaacct cataggctaa cacatcaata
     1441 agtaattgac gaaaatttaa acgccagcct aagtttttgt cgtttttaac aattttccag
     1501 ccttctaact catgttttgc gatatagtta ttgacgacat tgactgtttc atccgttgaa
     1561 caatcatccc tcaataatac ataatctggt tttaatgtct gttggcgaat tgaatcaagt
     1621 tgttcagata taaatttttc gccattgtat gtcgccatta aaactgctgt ctttaccata
     1681 ttactcctca aatgcttgta ctgtttttaa tattccagat tcaagagaaa cttctcgctt
     1741 ccatcccaac gcttctaatt tggccatagt gagacgtgtt ctccccattt tgctataacc
     1801 tgcactcata gttttaggaa tatcgaatac tactgaaata tttttatcag aaaagagatc
     1861 aactaacttt tgtgccaaat ccttaatcat aatgggttga tcttcatttg ctacattgta
     1921 agcttgacca acttcaccat ttaataaaat agtaaataac cctgatacag catctgctaa
     1981 ataacagaaa gcacgctctg ctgtaccact actttttaaa acgatatctt tgccatcaat
     2041 gacattcgat aaaagatcat tcataatacg accatcgtta ccaagctcca taccaggacc
     2101 aaatgaatgt gcaatacgag caatagtaaa gggtacctta tattgatcat agtaactttg
     2161 cagtagagtt tcagccatac gtttgctttc tgggtaacaa gctctagtag ctaaaatatc
     2221 aaatccacca taagcttctt catcaatcac ttctttaata gatgttccat atacttcacg
     2281 cgttgataag aacaagaagt tttcaattgt tttttccttt gcaaagtcta ataaattcaa
     2341 tgtaccaatt gtattagctt ttatgatgct aacaggatta ctcaaaatag cagttggact
     2401 agcattgctt gctgcatgga taatgtaatc cacttttcca tcatactcta ggcgttcttc
     2461 tacatcataa ggaattaagg tgaaataatc tttcccaact aaatctgaaa atttatcacg
     2521 ggctttttca atgtttctag ctgtcgcaat aatttgcgtt tggtaatttc ttgtctcatt
     2581 gagataggct agtaaaaata ccatataact cgctaacata ctattagaac cacttattaa
     2641 aacgccttta ttagcaattc tttcccaatt aatataattt tggttactaa taagtgctaa
     2701 atcttcttgt acaattttat tactaaattt aatcatcatt acatcccata aatttgttca
     2761 ttttctctag catcatacaa agctttaaac atataaaaat catctggtgt tgttatcttg
     2821 atattttcat atggtccctc aacaatagtg agttcacgat tgtacatacc catcaaagta
     2881 ctagaatcaa ttgcatcagt aatccctttg ctaatggcat cacgttcaac agaaaggata
     2941 tctgatagat aaaaactttg tggcgcttta gcaatgaagg aacgagtacg atcaacaact
     3001 tcagatattt tagaagaatc attgactaga acaactgtct ctttagcacg aactgaagtt
     3061 accgcacttc ctgtttcttt tactgactgt atattagcat taataacttc ttcattaatc
     3121 aatggacgta ctccgtcatg aattaaaaca acggcatcat ctgtagccag ctgttcagcg
     3181 gcttcaagcc ccttaaaaat tgacatttgg ccagtttctc cacctgcaac aactgctttt
     3241 actttcgtaa ggttgaaacg ctctaccaga ttattcatat aatctagcca gtctgataca
     3301 cagacaacta ctacagcatc aatagcttct gtattttgga aaatatcaat tgtgtgtaca
     3361 ataatgggtt taccatgcac ttctaggaat tgctttggtt tacctttggt attcatgcga
     3421 cgaccaacac cacctgcaaa aataactcca atattcatca ttaaccttct ttccagtacc
     3481 aagtgtaata actatgactt tctctcttac tttctggagg taattccata taactacccc
     3541 catataattg attcagataa gcatgctcat ttttaatttt tcgggtttta atgttttcaa
     3601 acataacatc ttcatactca gaaaaaattt ctcttggaaa atattctttc aagcgatatc
     3661 ttgaatcggt ataacccatg taaggaacct cttgattatt atatgtttcc atcacttgat
     3721 caacaatttc agcacgtttt ttagcttggc cgtgataagc gatgaatcgg ggtagcccca
     3781 aaattagcct tgcatttgca taaagctttc gggaaccgct agcaaaattg gggtaagtcg
     3841 tacacatcaa atttgcagca gcagagtgaa cttttttaaa gaatctttga cgttcttctg
     3901 cactctctgg taatcggtca agtgggaaga tatcaataaa aatccctgtc caaggatgaa
     3961 ttctatctgt tttacttaag tacattgact ttgtgtgata cagcttggca aatggccata
     4021 gatttttttc aacagaatga tgtagcaatt taaaatcgct attattagca tcagctaatg
     4081 ctttcatcaa acgctcatac tggcttcttt ctagcatcaa atcaatatcg tcatcccatg
     4141 gaatgaatcc tttatgtctc attgctccca gtaggctgcc tccgcctaaa gaatattcaa
     4201 ttttattatc acgcgcaact ttatcaatat aagctaacat ttcaagttga acttctctta
     4261 tttctgagac cgtcatttct ttcataatta cctctgtcta acagttaact cacattcttt
     4321 taaatacaaa tcttatagct gtaatataga aaataaataa agctactgca aaaagaatca
     4381 ctagggtcat gggtttgaaa gttttctttg ttttttgacc ttttggagtg tttgcaagtg
     4441 cattgtattc ttgtttgttg tactgcatgt ccattatata accatttata ttatagttac
     4501 caaattgtgc tttagaaaca ctatctttat ttactgtaaa ataaaatggt ttattgcttt
     4561 gtaagtagcc ttttggagat tttgtctgta ttaagaaata cttatactta ttagacaaac
     4621 ctcgtaattt aaataatgat tggttacttt tcacttgttt tttatagtta taagcccata
     4681 acctaggatt caagtctatg tcatttgaat agccatcaat acctataatg ttatttcctc
     4741 taatatggta agcattttgg ttaagatatt ctttatttaa actcttaatc gttttttgag
     4801 tatttatcca aaagccatta ggttgatata gagatacttt tttatctgca ttcaatcttc
     4861 ctaaaatata ctctgaacct tgcaataatc tattttgatt gtcgtcatac atggcaaaat
     4921 ttataccata actgctcaaa ctaggtgtct ttatatagcc tttagaatcc tcgacaaaaa
     4981 tcccttgatc ttgtttttga ttatcaacag aaattagact ccgtttagtc tctatgacgc
     5041 ctttcttttc ctgagcaaca gacaaattat aatctctttt tgaagcaaca tgtcctgtaa
     5101 tctctaactc tttaatatca gcatttgtca aatcaaagtc taacttatca atagagtgaa
     5161 gtttatacag gctattttgt aataattctt tttgctctgt ttcatcacca ctactattta
     5221 aaacaatttt atcaccatcg acactaatac tgctatctat tggatacaaa cgtccctctt
     5281 taccttcttc cgcaataata ggcgcttgag aaagtcggta attataatta gtactatcta
     5341 ctacaaaatt agggctaact ctgacagtca gctgctttga ataaattgga atcctataag
     5401 taatacgttt accgtattca actcctatag tatctctttt tttgttatta acagttactc
     5461 gaggattatc tactcgggta ttaccaagat aaatcgtttc gtttttctga atatttttca
     5521 acacatattc ttgagtttct gcgctgtttt caaaagcagt taaaccaact ggaatattac
     5581 caattgcttt tcctttgtta tttgtgaact tataacgttt attttctgga ttatataaaa
     5641 cacttggctc ttctaagcca ttcatgtaaa ttaaataatc tttattcttt tcaagcagta
     5701 accttacaat agcaatagca tcctgtctag tattttttat accagaacca aatctatagt
     5761 tattatccat aaggactttt ttaccactaa cttggtactt ccgcttgata ctatcaaaag
     5821 tatagtgtgt aaagtctttg ctctcaaact cccccgtcct cactttatcc aatgctgttt
     5881 gtaaatgaat agcattaatc tgaacactgt attgcggggt attattatga taataataag
     5941 ctgtaatatc tactttttta tctgttttta cttttttatt attttggcaa cctactaaaa
     6001 aaagcatgaa aaacagacaa ataaaaatat gttttttact catctaaagt gctatcctct
     6061 atctcttttt tcaacaaaga aacctcttga atcaggcgtt taatttgctc atctcttttg
     6121 gtattgctac tagtatcaaa taattctaaa caaaacagaa ataaaatagc accaaataac
     6181 aagaaattca tcgttaaagc gaatccaagg tgatgagcga taaatgagac caccccagga
     6241 aatacactaa taaagactag aattaaacca actaatagcc aaggtaaaga acgtttaaca
     6301 gaaaatgtgt ttttattaac cattcttata atcatataaa gtaggacaat ggctaaaata
     6361 aacatttcaa tttgcaatgc taaactcatt tatcatcctc cttaataaac cctgcaataa
     6421 taattgatgt taaaacatcc gtcatataaa aaatcgattt tagggaactt atacttgaag
     6481 tacctgcctc acgttcaaac atattagctg tgacttcctt caaacgatag ccttttttca
     6541 aaatccgagc agttgattca ggctctgggt attgtactgg ataatgacac gataaataag
     6601 ctattagcgc cgcgttggct gccctataac cacttgttgt atcatagact ttgtgtcccg
     6661 tcgttaattt tatagcagca gatattaaat ttatgccaag acgcctcatt gctgtcgatt
     6721 gaaaattttg atgtttttta tcaacaaagc gagaaccaat aacaaaatct gcttcgtcat
     6781 ttaagattgg cataacaaca tcttcaatgc ttctaatatc atgttgacca tcaccatcaa
     6841 attgaacggc aacatcgtaa tgattacgat tagcgtataa gtatcctgtc tgcacacagc
     6901 caccaatacc tagattttga acaaggtcaa tatgatttaa acccaatcta tctaataact
     6961 ctggagtacc atcggttgac ccatcgttaa taacaatata atccagctca aaaggtagtg
     7021 agcgagacgc cttaaaatct acaatagtct ggactgtctt tgcaatacta ccttcttcat
     7081 tataagcagg tatcactagt aaaatttttt tcatcatctt atctcttatt gtaataattt
     7141 gttgcaatca accatttaaa cacaacttga tgcttaaatt gatttttgct ataaccatat
     7201 ttccataacc accttaatct tgtcataaaa ggctgtttgt caatttttat aaaacattta
     7261 atgagctcaa gatcattagc tgtcataata tcaccatact tatcaacaat taaggaagct
     7321 tgcttttgtg agtcatggat aagtttccaa taccttgtaa atatagattt aggaccttca
     7381 cgaagtatct taaatctctt atccattgtc ctcgccccca aaacattatt gtcatgttga
     7441 cggtatagtt gagttggtaa atccaaataa atgatttctc ctaaacttgc tgctaagagt
     7501 gctaaaaacc aatcatgcat taagatatca ttaggagtaa accacttctc agctaatgca
     7561 tgatttatca tcatagtccc accagtaaca gtattctcag ttagttctgg taataatgtc
     7621 gtattggcat ggtgcgattg agcccgaatc atgctgtctt gtaaaatatt taattcttga
     7681 ttaacaactt tcaaatcagt ataaacaagt aatggtacat cagatgcttt atgctttgct
     7741 gcctctaaac ttacagataa cttttctggc aaccatacat catcttgatc actaaaaaaa
     7801 tagaaatctg caacttcgta attaactagc gtaaaaaatg atttaataac tcctaaatta
     7861 tggtgctcat tttcattaat gaaacgaatt cgagaatctt tagcagtaaa gtttctaata
     7921 atatcacatg tcttgtcact tgaaccatca tctctgatta ggaggttcca ctctttaaag
     7981 gtttgctttt ggatactttc gatttgctgc gccagaaact tctctccatt gtaggtggcc
     8041 attaaaatat taactttcat ttaaaaacaa atcctcatat tcttcaacaa tttttttcca
     8101 agtatagttt tcttgaataa tggcttttgc tcgatcaccg taagtagata aatcctgttg
     8161 ttgatcacta tcgttaataa gcttagctaa atccccctct tttttagtcc agtaaaaact
     8221 tgagagacct gctactgatt tgttgaaatc aacatctaga acaagattca aatcagtatt
     8281 agctaaagcc tcaagcagtc ctggattagt accgccaact tcatgcccat gaatataagc
     8341 aaaggcttgt tgacgaacat aatccagcag atctttttca tagagcgtac ctacaaactt
     8401 aactctttta tcttgttgaa ggtttgtctt taaggacaac ttttcaaagt aggggttatt
     8461 ttgatggtta cagataataa ctaaatcacg cttagtatct gaagccatga actccctaat
     8521 agccgtttca taattgtttt cagggacaaa tcgaccaacg attagatagt aacccttaga
     8581 cttaatattc cattttttaa acaactgttt gacacgtgga tcatctgacg ataatttcct
     8641 agaattaatc tctgtaccgt aagcaatgaa ccttgtcttc acattagagt aggtagattg
     8701 aatgtaattt tcaataccaa tattatcaga aataattagg tctgcatttt tagtcataca
     8761 tttttcggcg tattttaaat aacgctgtgt gggacgagac cactttgatc gcttccactc
     8821 taaaccatcc ggattaacat acaataagcc accgacttta tgtattttat tggcaaaatg
     8881 ccaaataaag gcaccaattg tatttcctaa aatataaaaa ataggctctt ttaaatcatg
     8941 tgtcttaacc aagtcaaggg cataattaat ggccataata tcataagcaa tcacacgtgc
     9001 tggccctaat tggggaggat ttatagtaaa acaatcagcg tcagcaaagt taaaatgagt
     9061 atgatgttct ttatcactaa ggcatgcaac atggtatttt atgtcggaac ttttttgatg
     9121 attaatcaat tctgaaacaa aagtttcaaa accaccgtaa tgagctggca accctctact
     9181 tcctataatg aaaacatctt gcatcatctt ctccattcta gctaaacaat ccatacaatt
     9241 ataccataaa ttagaaaagt tagctaaaaa gagcagtaag ctaaaaggat tctctagcct
     9301 tactactctt tgttttgatc ttatttttta acttcctgct tgtagaactc ttttaaagca
     9361 tcttgccaag taggaatcac aaatcctgtt gctttcgctt tttccaaact catagttgaa
     9421 tttaaaggac gttttgcttt tgctgggaat tgactagaat caacaggctt aacctccaca
     9481 tctgtgtctt ttaagatttc aaccgcaaaa tcataccatg ttgtatcttc tttagcatca
     9541 tttgataaat gatagtaacc aaaatctttt tgattttcag ctaaataagt cataaactca
     9601 gccaaagtac gtgtccatgt tggtcgtcca tgttggtcgt taacaacagt tagagttttg
     9661 tgtgtttttg caaggttttg catcgtaaag acaaagtttt taccatagtt accaaatacc
     9721 cacgctgtgc gaatggtata gaattttgaa gtatattttt caactaactc ttcacccata
     9781 cgttttgtac gaccgtactc tgttttggga tcaggcagat catctacttc ccattcttgc
     9841 cccactggtt tttcaccatc aaacacgtag tccgtcgaaa tgtaaacaag tgttgcatca
     9901 tgtttagcag cagccttagc gacattctct gtaccagtga cattgattgc aaagtctagc
     9961 tcttttcctt catcttctgc cgcatcaaca gctgtgtaag ctgcacaatg gtaaactaaa
    10021 gatggcttca cctcttcaaa gactttgtcc accatctcgg catttgtaat atccatttca
    10081 gctacatcaa cagcaacata ctcttgagtt cgttcgtcta aaaggtgacg taattcacta
    10141 cctaattgcc cgtttgcccc tgtaattaaa atcattcctt gctcctttaa aaataagttt
    10201 aacttttaat tcttcgttta ttgtacacta aaaaagggaa tatttcatcc ctttactctt
    10261 tatcttatga acgaattccc aaagcgatac gagcataacg actcatctta tcaacagacc
    10321 aggctgggta ccataccaat ttcacctcgg tttcagttac ctctgggact gttttcataa
    10381 catcatgaat ttgatctgtc aataaatctg ctaacgggca tcccatagtc gttaaagtca
    10441 tatcaatttc agttcgaccg ttatcttcaa agcgaatctc ataaattaac ccaaggttaa
    10501 caatatctat tcccaattca ggatcgataa ccatctccaa agcttctagg atacgatctt
    10561 ttatttttcc aacttcttct tcgctatatt gctttacttc tgacattata atacctctct
    10621 gaaaatatta ataccgtctt taagataact cctttgcaaa tcatttttat tagaaactaa
    10681 acttcctatt ctaaaaacga ttatcgagaa gcaatcaatc ttccatgaaa tctttaagtt
    10741 gcttgctacg actaggatgg cgaagttttc taagggcttt agcttcaatc tgacgaatgc
    10801 gctcacgagt aacattaaat actttaccta cgtcctctaa cgtacgcatt ttaccatcgt
    10861 caagaccaaa gcgtaaacga agaacattct cttcacggtc agtaagagta tctaaaacct
    10921 catctaattg ctcacgaaga acaacacgtg tagtataatc tactggattt tcaataactt
    10981 cgtcttcaat gaaatcaccc aaatggctat catcttcctc accgattggc gtttctagcg
    11041 ataccggctc ttgagcaatt tttaggattt cacgtacttt atctggtgtc atatccatgc
    11101 gttctgcgat ttgctcagga gttggatctt gaccaagttc ttgaagaaga ttacgttgtt
    11161 cacggaccaa tttattaatc gtttcaacca tatgaacagg aatacgaatc gtacgtgctt
    11221 gatctgcaat tgcacgtgtt atagcttgac gaatccacca tgttgcataa gttgaaaatt
    11281 tgaatccctt tgaataatca aatttatcaa cagctttcat gagccccatg ttaccctctt
    11341 gaattaaatc aagaaattgc attccacgtc caacataacg ttttgcaatt gagacaacaa
    11401 gacgaaggtt tgcttcagct aaacgttgtt tagccattaa atctccctca gcaaccgcaa
    11461 cagccaattc tttttcttct tcattcgtta aaagaggaac aacaccaatt tccttcaagt
    11521 acatacgtac tgggtcattg actttagcag aattacttcc tatgagctct tcatcagtca
    11581 attcttcagg ttttggccct tctaccacat actttgttga aggattgcct tctttatctg
    11641 tgattgaaat gcccccgtct gtcaatcgtt ctaacaaatc atcaatttga tctgcatcta
    11701 aaacaaaagg aataacaagt ttttctgtta cctcatcatc aattgcagta ccttgttttt
    11761 tatgattgcg aataaaatcc gcaacttgaa cattaaaagt tgtatttcct tttttctctg
    11821 ccataggttc ctcattccat atttcttttt tgagcaatta aattttctaa tgcagccaag
    11881 gctccttctt catcaccttg attactagat tgtctaatta actggctttg ttttcttaaa
    11941 tcacgttctt tcaataaacg gtcacgtgcc ttttcgacgg cttcaatctc tccaatagat
    12001 acttctacag gtaattgttc ctctaaaatt atataataag tacgattaac tttgtcactt
    12061 tcttgtgata aatcataaga cgtgatctca ccactttctt ttagcaactc atataaaact
    12121 tgtatttctg ttgtatcaaa ataaaaatta tccctattac ggaattcatt taacaggtag
    12181 ggatgattca ataatcgatg aaacaattgc atttctgttt tagtgatagc tgataactta
    12241 cctgtctgtg gtaattgcac tgtagcactt gtttgtactg aagatgaagc ttgctgttga
    12301 cttctatgat gcaatcgttc gttattaacc gattgttcta cttgaaaata atcaaaatct
    12361 ggcaagagtt cagccacttt tgtaatatat gaattttgtg ctgttatcga tggagattta
    12421 gcaattaatt tcgctatttt ttcaacataa gcaatttcag attgtaaatt atcggtgttt
    12481 tcaggcttta agtagtggat ataaaactct gtattactga tgcgcccatt ttctaagagt
    12541 tgtttaaaat cttctgctga atttgcttgt agaaattcat cagggtccat tttattaggt
    12601 atcctaacga tatcaacagt catatctgat agcagttcta aagacttgtc aattgcgttt
    12661 tgaccagctc tatcaccatc ataagtcaga acaactttct tagtaaatct tttaaggtgt
    12721 ctaacgtgtt cattggttaa agcggtcccc atagaagcaa caacattctc aattcctgca
    12781 cgataagctg caataacatc cataaagcct tccattaggt aaacttcgtg tgctttatta
    12841 attacagcac gcgctttatc taaatggtaa agctcgtaag acttattaaa aataggggtg
    12901 gcacgagaat ttttatattt agcttgtttt ctatcaatgt cctcttgagt ccaaactcgt
    12961 cctgaaaacg caatgacttc accaatatca ttagttaacg gaaacataat tctattatta
    13021 aatgaatcgt aaaactgatt gctattttct gaataattga acaaaccaga agacatcaaa
    13081 gttttttcat caaactcttt tgccagacga cgatatagaa aatcctgccc tcccggcgat
    13141 aatcctattt gaaaatgttt aattaagtcc tctgtcactc cgcgctcggc aaggtattgt
    13201 ctagcgcgtt caccatcatt agttgtcatt aaataggcat ggtaaaatcc cgaagcaact
    13261 ctatgaatat ctaaaagcga ctgattagct ttaggttttt taggaacttg ccccttaaaa
    13321 ttagtatcta gaggtattcc tgaacgttca gctagaactt gtacactatc taaaaaacta
    13381 atatgttggt aatcttccac aaatttaaag acatcaccag agcgaccaca accaaaacaa
    13441 tgaaaaaact gcctatcttc gataacatta aaagatggcg ttttttcttt atgaaatggg
    13501 cataaaccaa gatgattgcg tcctgttttt gtcaaaccga caacttcacc gatgacatca
    13561 acaatgttaa cactgttctt gatttcagaa attttttctt tatcaattgc caagtcatga
    13621 cctccacaga aataacccat tatagtttta catttgatta tatcattttt tcttttctgt
    13681 gtaaattttt tcaaaagctt tttcaaggat ttcttcttca ctgatttaat ataaaaattt
    13741 acagataatt tgaaacctat tacttggatt tctcaaaaga aatctgctat aatgaacgtg
    13801 aattaaataa tattaaggag cttgttcaca tgattaaaga actaaaagaa tttttattta
    13861 agggtaatgt tttagacctt gctgttgctg ttattttagg tgcagctttt aatgctatca
    13921 tcacttcact tgttaaagac gttattacac cgcttatcct taatcctgta ttaaaagctg
    13981 ctggcgtttc aaatatcgca cagctttcat ggaatggtgt tgcttacggt aatttcttaa
    14041 gtgcagttat taatttctta atcgttggta ctactttatt cttcatcgtt aaagctgcta
    14101 acaaggttat ggctaagaaa cctgctgaag aagaaattat tgaagttgtt gaaccaacac
    14161 aagagcaatt acttgctgaa atccgcgact tactcgctaa caaataataa aagactcgga
    14221 tta
//
LOCUS       DATRUK010000056        14032 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.056, whole genome shotgun sequence.
ACCESSION   DATRUK010000056 DATRUK010000000
VERSION     DATRUK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14032
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.056"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..2034
                     /locus_tag="VBE34_001556"
     CDS             <1..2034
                     /locus_tag="VBE34_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO7264262.1"
                     /translation="LVAGTSIMFSSVFADQVGVQVIGVNDFHGALDNTGTANMPDGKV
                     TNAGTAAQLDAYMDDAQKDFKQTNPNGESIRVQAGDMVGASPANSGLLQDEPTVKTFN
                     AMNVEYGTLGNHEFDEGLAEYNRIVTGKAPAPDSNINNITKSYPHEAAKQEIVVANVI
                     DKVNKQIPYNWKPYTIKNIPVNNKSVNVGFIGIVTKDIPNLVLRKNYEQYEFLDEAET
                     IVKYAKELQAKNVKAIVVLAHVPATSKDDIAEGEAAEMMKKVNQLFPENSVDIVFAGH
                     NHQYTNGLVGKTRIVQALSQGKAYADVRGVLDTDTQDFIETPSAKVIAVAPGKKTGSA
                     DIQAIVDQANTIVKQVTEAKIGTAEVSGMITRSVDQDNVSPVGSLITEAQLAIARKSW
                     PDIDFAMTNNGGIRADLLIKPDGTITWGAAQAVQPFGNILQVVEITGRDLYKALNEQY
                     DQKQNFFLQIAGLRYTYTDNKEGGEETPFKVVKAYKSNGEEINPDAKYKLVINDFLFG
                     GGDGFASFRNAKLLGAINPDTEVFMAYITDLEKAGKKVSIPNNKPKIYVTMKMVNETI
                     TQNDGTYSIIKKLYLDRQGNIVAQEIVSDTLNQTKSKSTKINPVTTIHKKQLHQFTAI
                     NPMRNYGKPSNSTTVKSKQLPKTNSEYGQSFLMSVFGVGLIGIALNTKKKHMK"
     gene            complement(2071..2481)
                     /locus_tag="VBE34_001557"
     CDS             complement(2071..2481)
                     /locus_tag="VBE34_001557"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO7264263.1"
                     /translation="MIKPIVRDTFFLQQKSQMASRADVSLAKDLQDTLHANQNYCVGM
                     AANMIGSLKRVIIINVGITNLVMFNPVLVAKSDPYETEESCLSLVGCRSTQRYHHITI
                     SYRDINWKEQQIKLTDFPAQICQHELDHLEGILI"
     gene            complement(2551..3900)
                     /gene="gdhA"
                     /locus_tag="VBE34_001558"
     CDS             complement(2551..3900)
                     /gene="gdhA"
                     /locus_tag="VBE34_001558"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEO7264264.1"
                     /translation="MTSGKTYVASVLEKVKKQNEHEEEFLQAVEEVFESLVPVFDKYP
                     QYIEENLLERLVEPERVISFRVPWVDDKGQVQVNRGYRVQFSSAIGPYKGGLRFHPTV
                     TQSIVKFLGFEQIFKNSLTGLPIGGGKGGSNFDPKGKSDNEVMRFTQSFMTELQKYIG
                     PDLDVPAGDIGVGGREIGYLYGQYKRLNGYQNGVLTGKGLTYGGSLARTEATGYGAVY
                     FAKEMLAARGQDLTGKVALVSGSGNVAIYATEKLQELGATVVAVSDSSGYVYDPDGID
                     LETLKQIKEVERARIVKYTEKHPKANFTPADQGSIWSIKADLAFPCATQNELDEEDAK
                     LLVENGVLAVTEGANMPSTLGAIKVFQKAGVAFGPAKAANAGGVAVSALEMAQNSSRR
                     AWTFEEVDQELQRIMKTIFVNASKAADEFGDSGNLVLGANIAGFLKVAQAMSAQGIV"
     gene            4068..4577
                     /locus_tag="VBE34_001559"
     CDS             4068..4577
                     /locus_tag="VBE34_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEO7264265.1"
                     /translation="MKWFPLVGGIIALLAGFYLFINPVTVVATLGWFIALFIFIYGIS
                     NLVRYLQADSYHRSPWYLVQSILAIIFGIILLSSSAMSLSNTVMTILAYWLLVSGILR
                     LLGGFQMQKINFPGAKSYKRSAYIALILGIILLVQPTLSAAIIGRFVAFILIAIGASA
                     IYTFTRINY"
     gene            complement(4618..6387)
                     /locus_tag="VBE34_001560"
     CDS             complement(4618..6387)
                     /locus_tag="VBE34_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7264266.1"
                     /translation="MRKKSIFLRLWSYLTRYKATLFLAIFLKVLSSFMSILEPFILGL
                     AITELTANLVDMAKGVSGAELNVPYIAGILIIYFFRGVFYELGSYGSNYFMTTVVQKS
                     IRDIRHDLNRKINKVPVSYFDKHQFGDMLGRFTSDVETVSNALQQSFLQIINAFLSII
                     LVVVMVLYLNVPLAMIIIACIPVTYFSAQAILKRSQPYFKEQAKILGELNGFVQEKLT
                     GFNIIKLYGREEASSQEFRDITDNLRHVGFKASFISGIMMPVLNSISDFIYLIIAFVG
                     GLQVIAGTLTIGNMQAFVQYVWQISQPVQTITQLAGVLQSAKSSLERIFEVLDEEEEA
                     NQVTEKLSHDLTGQVSFHGVDFHYSPDKPLIRDFNLDVEPGQMIAIVGPTGAGKTTLI
                     NLLMRFYDVSEGAITVDGHDIRHLSRQDFRQQFGMVLQDAWLYEGTIKENLRFGNLEA
                     SDEDIVAAAKAANVDHFIRTLPGGYNMVMNQESSNISLGQKQLLTIARALLADPKILI
                     LDEATSSVDTRLELLIQKAMKKLMEGRTSFVIAHRLSTIQEADNILVLKDGQIIEQGN
                     HQKLLADKGFYYELYNSQFSNSK"
     gene            complement(6392..8131)
                     /locus_tag="VBE34_001561"
     CDS             complement(6392..8131)
                     /locus_tag="VBE34_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7264267.1"
                     /translation="MAYIWSYLKRYPNWLWLDLLGAMLFVTVILGMPTALAGMIDNGV
                     TKGDRTGVYLWTFIMFIFVVLGIIGRITMTYASSRLTTTMIRDMRNDMYAKLQEYSHH
                     EYEQIGVSSLVTRMTSDTFVLMQFAEMSLRLGLVTPMVMIFSVVMILITSPSLAWLVA
                     VAMPLLVGVILYVAIKTKPLSERQQTMLDKINQYVRENLTGLRVVRAFARENFQSQKF
                     QVANQRYTDTSTGLFKLTGLTEPLFVQIIIAMIVAIVWFALDPLQRGAIKIGDLVAFI
                     EYSFHALFSFLLFANLFTMYPRMVVSSHRIREVMDMPISINPNTEGVTDTKLKGHLEF
                     DNVTFAYPGETESPVLHDISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYDVTLGKIL
                     VDGVDVRDYNLKSLRQKIGFIPQKALLFTGTIGENLKYGKADATIDDLRQAVDISQAK
                     EFIESHQEAFETHLAEGGSNLSGGQKQRLSIARAVVKDPDLYIFDDSFSALDYKTDAT
                     LRARLKEVTGDSTVLIVAQRVGTIMDADQIIVLDEGEIVGRGTHAQLIENNAIYREIA
                     ESQLKNQNLSEGE"
     gene            complement(8198..8671)
                     /locus_tag="VBE34_001562"
                     /pseudo
     CDS             complement(8198..8671)
                     /locus_tag="VBE34_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(8668..10536)
                     /locus_tag="VBE34_001563"
     CDS             complement(8668..10536)
                     /locus_tag="VBE34_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO7264268.1"
                     /translation="MSDFLVDGLTKSVGDKTVFSNVSFIIHSLDRIGIIGVNGTGKTT
                     LLDVISGELGFDGDRSPFSSANDYKIAYLKQEPDFDDSQTILDTVLSSDLREMALIKE
                     YELLLNHYEESKQSRLEKVMAEMDSLDAWSIESEVKTVLSKLGITDLQLSVGELSGGL
                     RRRVQLAQVLLNDADLLLLDEPTNHLDIDTIAWLTNFLKNSKKTVLFITHDRYFLDNV
                     ATRIFELDKAQITEYQGNYQDYVRLRAEQDERDAASLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFQNLKNDLHQTSDTSDLEMTFETSRIGKKVINFENVSFSYPDKSILKD
                     FNLLIQNKDRIGIVGDNGVGKSTLLNLIVQDLQPDSGNVSIGETIRVGYFSQQLHNMD
                     GSKRVINYLQEVADEVKTSVGTTSVTELLEQFLFPRSTHGTQIAKLSGGEKKRLYLLK
                     ILIEKPNVLLLDEPTNDLDIATLTVLENFLQGFGGPVITVSHDRYFLDKVANKIIAFE
                     DNDIREFFGNYTDYLDEKAFNEQNNEVISKKESTKTSREKQSRKRMSYFEKQEWATIE
                     DDMMILENTITRIEDDMQTCGSDFTRLSDLQKELDAKNEALLEKYDRYEYLSGLDT"
     gene            complement(10547..11755)
                     /locus_tag="VBE34_001564"
     CDS             complement(10547..11755)
                     /locus_tag="VBE34_001564"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEO7264269.1"
                     /translation="MRLNYLPSEFQKALPILKKIKKAGYEAYFVGGSVRDVLLDRPIH
                     DVDIATSSYPEETKQIFKRTVDVGIEHGTVLVLEKGGEYEITTFRTEEVYVDYRRPSQ
                     VNFVRSLEEDLKRRDFTVNAFALNEDGEVIDLFHGLDDLDNHLLRAVGLASERFNEDA
                     LRIMRGLRFSASLNFDIETTTFEAMKKHAPLLEKISVERSFIEFDKLLLAPYWRKGML
                     ALIDSHAFNYLPCLKNRELQLSAFLSQLDKDFLFETSEQAWASLILSMEVEHTKTFLK
                     KWKTSTHFQKDVEHIVDVYRIREQMGLTKEHLYRYGKTIIKQAEGIRKARGLMVDFEK
                     IEQLDSELAIHDRHEIVVNGGTLIKKLGIKPGPQMGDIISQIELAIVLGQLINEEEAI
                     LHFVKQYLMD"
     gene            complement(11940..12788)
                     /locus_tag="VBE34_001565"
     CDS             complement(11940..12788)
                     /locus_tag="VBE34_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO7264270.1"
                     /translation="MKLALITDTSAYLPEAIENHEDVYVLDIPIIIDGKTYIEGQNLT
                     LDQYYDKLAASKELPKTSQPSLAELDDLLCQLEKEGYTHVLGLFIAAGISGFWQNIQF
                     LIEEHPNLTIAFPDTKITSAPQGNLVRNALMCSREGMDFDVIVNKIQSQIEKIEGFIV
                     VNDLNHLVKGGRLSNGSAIIGNLLSIKPVLHFNEEGKIVVYEKVRTEKKALKRLAEIV
                     KEMTADGEYDIAIIHSRAQDKAEQLYNLLAKAGLKDDLEIVSFGGVIATHLGEGAVAF
                     GITPKN"
     gene            complement(12788..13168)
                     /locus_tag="VBE34_001566"
     CDS             complement(12788..13168)
                     /locus_tag="VBE34_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEO7264271.1"
                     /translation="MEVIREQEFVNQYHYDARNLEWEEENGTPKTNFEVTFQLANRDE
                     AAKVTSIVAVLQFVIVRDEFVISGVISQIAHIQGRLINEPSEFSQDEVENLAAPLLEI
                     VKRLTYEVTEIALDRPGVTLEFNS"
     gene            complement(13240..13773)
                     /locus_tag="VBE34_001567"
     CDS             complement(13240..13773)
                     /locus_tag="VBE34_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264272.1"
                     /translation="MTMKTIEEIYKNYLNIPYISPERDLAEINFSKVVPRKNMEETSE
                     GLLPGDIILLWCIQFGTFTTETSFSKYFEYIYGINGKEHLEFLIKNGFVRMESPLDSL
                     DHLSAPLLKLFLKEKNVKGLSKMKRSDLDQAIAIAFTEEELGKLFVVRGLALTEKGLA
                     ALSNNQEVIDRHPKKKF"
     gene            complement(13824..>14032)
                     /locus_tag="VBE34_001568"
     CDS             complement(13824..>14032)
                     /locus_tag="VBE34_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEO7264273.1"
                     /translation="VALILLHKEKLFAYPLSILVFVGFIIFQLREYFSSHSILMIFLC
                     VFDVIMIILTWLEYQKMKLNFSKR"
BASE COUNT         4545 a         2819 c         2156 g         4512 t
ORIGIN      
        1 ttagtcgctg ggacttctat tatgttctca agcgtattcg ctgaccaagt cggcgtccaa
       61 gttataggcg tcaatgactt tcatggtgca cttgacaata ctggaacagc aaatatgcct
      121 gacggaaaag ttactaatgc tggcactgct gctcaattag atgcttatat ggatgatgct
      181 caaaaagatt tcaaacaaac taaccctaat ggtgaaagca ttagagttca agctggtgat
      241 atggttggag caagtccagc taactcaggg cttcttcaag atgaaccaac cgttaaaaca
      301 tttaatgcaa tgaatgttga gtatggcaca ttaggtaacc atgaatttga tgaaggtttg
      361 gcagaataca atcgtatcgt tactggaaag gcccctgctc cagattctaa tataaataat
      421 attacgaaat catacccaca cgaagctgca aaacaagaaa ttgtagtggc aaacgttatt
      481 gataaagtta acaaacaaat cccttacaat tggaaacctt acactattaa aaatattcct
      541 gtaaataaca aaagtgtgaa cgttggcttt atcggaatcg ttaccaaaga catcccaaac
      601 cttgtcttac gtaaaaatta tgaacaatat gaatttttag atgaagctga aacaatcgtt
      661 aaatacgcca aagaattaca agctaaaaat gtcaaggcta ttgtagtcct tgctcatgta
      721 cctgcaacaa gcaaggatga tattgctgaa ggtgaagcag cagaaatgat gaaaaaagtc
      781 aatcaactct tccctgaaaa tagcgtagat attgtctttg ctggacacaa tcatcaatat
      841 acaaatggtc ttgttggtaa aactcgtatt gtacaagcgc tctctcaagg aaaagcctat
      901 gctgatgtac gtggtgtcct agatactgat acacaagatt tcattgaaac cccttcagct
      961 aaagtaattg cagttgctcc tggtaaaaaa acaggtagtg ccgatattca agccattgtt
     1021 gaccaagcta atactatcgt taaacaagta acagaagcta aaattggtac tgccgaggta
     1081 agtggcatga ttacgcgttc tgttgatcaa gataatgtta gtccggtagg cagcctcatc
     1141 acagaggctc aactagcaat tgctcgaaaa agctggccag atatcgattt tgccatgaca
     1201 aataatggtg gcattcgtgc tgacttactc atcaaaccag atggaacaat cacctgggga
     1261 gctgcacaag cagttcaacc ttttggtaat atcttacaag tcgtcgaaat tactggtaga
     1321 gatctttata aagcactcaa cgaacaatac gaccaaaaac aaaatttctt ccttcaaata
     1381 gctggtctgc gatacactta cacagataat aaagagggcg gggaagaaac accatttaaa
     1441 gttgtaaaag cttataaatc aaatggtgag gaaatcaatc ctgatgcaaa atacaaatta
     1501 gttatcaatg actttttatt cggtggtggt gatggctttg caagcttcag aaatgccaaa
     1561 cttctaggag ccattaatcc cgatacagag gtatttatgg cctatatcac tgatttagaa
     1621 aaagctggta aaaaagtgag cattccaaat aataaaccta aaatctatgt cactatgaag
     1681 atggttaatg aaactattac acaaaatgat ggtacatata gcattattaa gaaactttat
     1741 ttagatcgac aaggaaatat tgtagcacaa gagattgtat cagacacttt aaaccaaaca
     1801 aaatcaaaat ctacaaaaat caaccctgta actacaattc acaaaaaaca attacaccaa
     1861 tttacagcta ttaaccctat gagaaattat ggcaaaccat caaactccac tactgtaaaa
     1921 tcaaaacaat taccaaaaac aaactctgaa tatggacaat cattccttat gtctgtcttt
     1981 ggtgttggac ttataggaat tgctttaaat acaaagaaaa aacatatgaa ataataaaaa
     2041 taggtttgga taaaatatcc aaacctattt ttatatcaat atgccttcca aatgatctaa
     2101 ctcgtgttga caaatttgag ctggaaaatc tgtcaacttg atttgctgtt ctttccaatt
     2161 gatatcacga taagaaatcg taatgtgatg atatcgttgt gtacttctgc aacctacgag
     2221 agaaaggcaa gattcttctg tctcatatgg atccgatttt gcaactaata cagggttaaa
     2281 cattactaaa tttgtaatac ccacattgat gataatgaca cgcttcagac taccaatcat
     2341 attggctgcc atacctacac aataattttg attagcatgc aaagtatcct gtaaatcttt
     2401 tgctaagctc acatctgctc tactagccat ttgtgatttt tgttgtaaaa agaaagtatc
     2461 tctaacaatt ggttttatca tttattttaa cctaactcta ttatgataat actagtatac
     2521 cataggttag ctaaggacta tgataagaga ttaaacaata ccttgtgcag acatagcttg
     2581 agcaactttt aaaaagcctg caatgttagc acctagaacc aggttaccac tatcgccaaa
     2641 ttcatcagcc gctttggaag cattcacaaa aatagttttc ataatacgtt gcaattcttg
     2701 atcaacttct tcaaaagtcc atgccctacg actagaattt tgtgccattt caagcgctga
     2761 aacagctact cctcctgcat tggcagcttt agcaggacca aaagcaaccc ctgccttttg
     2821 gaaaacttta atagcaccca aggtagatgg catatttgct ccttctgtta ctgccaagac
     2881 accattttca actaataact tagcgtcttc ttcgtctaat tcgttttgag tagcacacgg
     2941 gaaagctaaa tctgctttaa tagaccagat actaccttga tctgctggtg tgaaattagc
     3001 ttttggatgt ttctccgtat atttaacaat acgtgctctt tcaacctctt ttatctgttt
     3061 caatgtttca aggtcaatac cgtctggatc ataaacataa cctgaagaat ctgacacagc
     3121 tacaacagtt gcacccaact cttgtaattt ttcagtagca taaatagcca cgttaccaga
     3181 tcccgaaacc agtgcaactt tgccagttaa atcttgacca cgcgctgcca gcatttcttt
     3241 agcaaaatag actgccccat aaccagttgc ttctgtacga gccaaagagc caccataagt
     3301 cagtcctttt ccggttaaaa ccccattttg ataaccattt aaacgcttat attgaccata
     3361 aagataacca atttcacgtc cacctactcc aatatcgcca gctggcacat ctaaatctgg
     3421 tccaatgtat ttttgaagtt cggtcataaa actttgtgtg aaacgcatca cttcattatc
     3481 tgatttccct tttggatcaa agttcgaccc tcctttgcca ccaccaattg gtagacctgt
     3541 cagtgaattc ttaaaaattt gttcaaatcc taaaaatttg acaatagatt gtgttactgt
     3601 agggtgaaaa cgtaaaccac ctttataagg accaatagct gatgaaaatt gtacacgata
     3661 tcctcggtta acttgaactt ggcctttatc atctacccat ggaactctaa aagaaatcac
     3721 gcgctcaggc tcaaccaaac gttctaaaag attttcttca atgtactgag ggtatttatc
     3781 aaaaactggt actagtgact caaaaacctc ttcaaccgct tgtagaaatt cttcctcatg
     3841 ctcattttgc ttcttcactt tctctaaaac acttgctaca tatgtctttc cgcttgtcat
     3901 ttattttcct ccgtattgtt ttaatatagt tagcattata aattgtcaga attttcttgt
     3961 caactatttg ctatggtatc attattagga gcaattttaa aggtaattat ttgactttta
     4021 ctgactttcg ttctataatg agacaaatat ttgattggag tatctatatg aaatggttcc
     4081 cacttgttgg aggtataatt gccttattag ctggcttcta tctctttata aatcctgtaa
     4141 cggttgttgc aactctgggt tggttcattg ctttatttat ctttatttat ggcatttcaa
     4201 atttagtacg ctacttgcaa gctgattctt atcaccgtag cccttggtat ctggttcaga
     4261 gtatccttgc tattattttt ggtatcattt tactatccag ctcagcaatg tcactttcaa
     4321 atactgtcat gactatttta gcatactggc tactggtatc tggtatttta cgacttttgg
     4381 gtggtttcca aatgcaaaaa attaatttcc caggtgccaa atcctataaa cgttcggctt
     4441 atattgcact cattttaggt attattttac tggttcaacc taccttatca gcagccatca
     4501 ttggaagatt tgtagccttt atattaatag caattggagc ttcggctatt tacactttta
     4561 ctcgtattaa ttattaaaag gacatgtttc gtgtcctttt tttatctatt gatgacgtca
     4621 tttactattt gaaaattggc tattatataa ttcgtaataa aatcctttat ctgctaggag
     4681 tttctggtga ttaccttgtt cgatgatttg accatccttt agaactaaaa tgttgtctgc
     4741 ctcttgaatc gttgatagac ggtgtgcaat gacaaaacta gttcttcctt ccatcaattt
     4801 tttcatagct ttttgaataa gcaattccaa ccttgtatca acagatgaag tcgcttcatc
     4861 taaaattaaa attttaggat ctgctaataa agcacgtgca atagtcaaca gttgcttttg
     4921 tcctaaggag atattgcttg attcttgatt catcaccata ttgtagccac caggtaacgt
     4981 tcgaataaag tggtcaacat tcgcagcttt ggcagcagca actatatcct catcactggc
     5041 ttcaagatta ccgaatctaa gattttcttt tattgtacct tcatataacc aagcatcttg
     5101 caacaccatg ccaaattgtt gccggaaatc ttggcgtgat aggtgtctaa tatcgtgacc
     5161 atctacggtg atagcacctt cactaacatc gtagaatctc atgagtaagt ttataagtgt
     5221 tgtcttacct gcacctgttg gccctacaat tgcaatcatt tgccctggtt ctacatctaa
     5281 gttaaagtcg cggataagtg gcttatccgg agaatagtga aaatcgacac catgaaagct
     5341 gacttgaccg gttaagtcat ggctcaattt ttcagtgact tgattggctt cctcttcttc
     5401 atctaagact tcaaaaatac gttctaagga agattttgca ctttgcaaaa cacctgctaa
     5461 ttgtgtaatg gtttgaacgg gttgactaat ttgccaaaca tactgcacaa atgcctgcat
     5521 gttaccaatt gttaaggtac cagcaataac ttgtaaaccg ccaacaaatg caatgataag
     5581 ataaataaaa tcagagatac tattcaatac tggcatcata attcctgaaa tgaagctagc
     5641 tttgaaacct acatgacgta gattatccgt tatatcacga aattcttgac tagacgcctc
     5701 ttctctacca tataatttta tgatattgaa accagttaat ttctcttgta caaaaccatt
     5761 cagttctccc agaatctttg cctgttcttt aaagtaaggt tgggatcttt ttagtatcgc
     5821 ttgtgcacta aaataagtta caggaataca agcaatgata atcatggcta atggtacatt
     5881 tagataaagt accataacga ctactagaat aatacttaaa aaagcattga taatctgtaa
     5941 aaagctctgt tgtaaagcat ttgagaccgt ttcaacatca cttgtaaaac gtcccaacat
     6001 atctccaaat tggtgcttat caaaatacga tacaggaact ttattgattt tacgattcaa
     6061 atcatgacgg atatcacgga tacttttttg aacgacagta gtcataaaat aatttgagcc
     6121 ataagaacct aattcataga aaacacctct gaaaaaataa ataatcagaa taccagcaat
     6181 ataaggaacg ttcaattctg ccccagaaac tcccttagcc atatcaacaa ggttagcagt
     6241 caactctgtt atcgctaacc ctaaaataaa aggctccaga atactcataa aactagataa
     6301 aactttcaaa aaaatcgcta agaaaagagt agctttgtag cgagttaggt aagaccataa
     6361 tctcaaaaaa atagattttt ttctcataca atcactctcc ttctgataag ttttggttct
     6421 tcagttgtga ctcagcgatt tcacgataaa tagcattatt ttctattaat tgagcgtggg
     6481 taccacgacc gacaatttcg ccttcatcaa ggacaataat ctgatcagca tccataatcg
     6541 tacccaccct ttgagcaact atcaaaaccg tagaatcacc ggttacttct tttagacgcg
     6601 ctcttaaagt agcgtctgtc ttataatcga gagcagaaaa tgaatcatca aaaatatata
     6661 aatctggatc tttaacaaca gccctagcaa tggataaccg ttgtttttga cccccagaaa
     6721 gattgctccc accttcagct aaatgcgttt caaaggcttc ttggtgactc tcaataaact
     6781 ctttagcttg agaaatatca accgcttgtc taagatcatc aatagtagca tcagcttttc
     6841 catattttaa attctctcct attgtccctg taaataaaag agctttttgg gggataaatc
     6901 caatcttttg gcgaagtgat ttaaggttat aatctcttac atcaactcca tctactaaga
     6961 tttttccaag tgtcacatca taaaaacgtg gaatcaaatt aacaagagaa gattttcctg
     7021 aacctgttga accaataaaa gcaattgttt ctccaggctt agctttaaaa gaaatatcat
     7081 gcaaaacggg actctctgtt tctcctggat aagcgaatgt tacattatca aattctaaat
     7141 gccctttaag tttcgtatcc gtaacacctt cggtattagg attaatagaa atcggcatat
     7201 ccattacctc tctaatacga tggctagata ccaccatacg aggatacata gtaaaaagat
     7261 tggcaaatag caaaaatgaa aagagagcat ggaagctata ttcgataaaa gcaactaaat
     7321 cccctatttt aatagcacct ctttgtaagg gatccaaagc aaaccaaacg atagccacaa
     7381 tcattgcaat aataatttga acgaaaagtg gttctgttag ccctgttaat ttaaaaagac
     7441 cagttgaagt atctgtgtaa cgttggttag cgacttgaaa tttttgtgat tgaaaattct
     7501 ctcttgcaaa ggccctaaca acgcgtaacc ctgttaaatt ttcacgaaca tattgattga
     7561 ttttatcaag catagtctgt tgtctttcag ataaaggttt tgtttttata gctacatata
     7621 aaatgactcc taccaaaaga ggcatcgcaa ccgctacaag ccaagccaaa gatggactcg
     7681 taattagtat cataaccacg ctaaaaatca ttaccatagg agttactagg cctaaacgta
     7741 aagacatttc agcaaattgc atcaaaacaa aagtatcgct tgtcatacgt gtcactagtg
     7801 aagatacacc tatctgttca tattcatgat gggagtattc ttgaagctta gcatacatat
     7861 cattacgcat atctctaatc attgttgtcg ttaagcgact agatgcgtaa gtcatcgtaa
     7921 tacgcccaat aatacctagt acaacaaata taaacatgat gaacgtccac agataaactc
     7981 cagtccgatc accttttgta acgccattat caatcatacc cgctaaggct gtgggcattc
     8041 ctaggataac cgtcacaaaa agcatagctc ctagtaaatc aagccataac caatttgggt
     8101 accttttcaa ataagaccaa atatatgcca tatatgtctc cttttctttc ctcttatctc
     8161 aaaataatta atccatgttc actaatccac tttcaactta taaatcttta atcatccaaa
     8221 tatccatagc tgtatgacct tgatcatttg ctattggttg cgataaggca caatatccca
     8281 tatgcttata aaccgcagtt gccctactca aaatactggc tgtctctaaa taaagttgtc
     8341 tatacccaat ttttcgagct tctacttcaa tcattttcac taaatcagta gcaagcccct
     8401 taccacggaa acgttctgca atgtacacct tctgcatctc tgcaattaga tttttcagcg
     8461 gaccaaagcc gcttcagcca ataatctcat ctctctcctc aatgacaaag aatcctgact
     8521 tctctatttt ttcgtagtat gaggtcaagt gatctaagtg agggtctgaa tatgctgtac
     8581 caggtttatc taaatcatag gcgcgtaaac tctgtcgaat taattgtgta actgcttggt
     8641 catcattttt aataatcgga cggataatca tgtgtctaac ccactaaggt actcataacg
     8701 gtcatacttt tctagaagtg cttcattttt tgcatctaat tccttttgta aatcagataa
     8761 ccttgtaaaa tcactaccac atgtttgcat atcatcttct atacgagtga tagtattttc
     8821 caatatcatc atatcgtctt caattgtcgc ccattcttgt ttttcaaagt aagacattct
     8881 tttacgactt tgcttttcac gacttgtctt ggtactctct tttttactga taacttcatt
     8941 attttgctca ttaaatgctt tttcatctaa ataatcagta taattaccaa aaaattcacg
     9001 gatatcgtta tcttcaaatg caataatttt attagccact ttatctaaaa agtaacgatc
     9061 gtgactaact gtaatcacag gaccaccaaa gccttgtaaa aaattttcaa gaacagttaa
     9121 tgtagcaata tctaagtcat ttgtcggctc atcaagtagt aacacgttag gcttttcaat
     9181 caggattttt aaaaggtaaa gtcgtttttt ctcaccacct gataattttg caatttgtgt
     9241 tccatgtgtc gaacgtggaa agagaaattg ttccaatagt tctgtcacac ttgttgtacc
     9301 gacactagtt ttaacctcat ctgcaacctc ttgcaaataa ttaataacac gttttgagcc
     9361 atccatatta tgaagttgtt gtgaaaagta acctacacgt atcgtttcac caatagagac
     9421 attacccgaa tccggctgta aatcttgaac aattaaatta agtaaggttg actttccaac
     9481 accattatct ccaacgatgc caatacggtc tttattttga attaacaaat taaagtcttt
     9541 caagatagat ttatctgggt aagaaaaaga gacattttca aaattaataa cctttttccc
     9601 aattcgactt gtttcaaatg tcatttccaa atcgcttgta tcgcttgttt ggtgtaaatc
     9661 gttttttaga ttttgaaaac gattaatacg agcctgttgt ttcgttgcac gagcttgtgg
     9721 ctgagtacgc atccaagcta gttcctgttt ataaagctgt ttctttttat gtaaactagc
     9781 agcatcacgc tcgtcttgtt ctgcacgaag tcggacataa tcctgataat tgccttgata
     9841 ttctgtaatc tgtgccttat ctaattcaaa aatacgtgtt gcaacattgt ctagaaaata
     9901 acgatcatga gttataaaaa gcactgtctt tttactattt ttcaaaaaat tcgttaacca
     9961 tgcaatagtg tcaatatcta agtggttagt aggttcgtct aagagcaata aatctgcatc
    10021 atttaataat acttgcgcta attgaacacg tcttcgtaat cctcctgata attcaccaac
    10081 cgacaactgc aaatcagtaa tacctaattt ggataatact gttttgactt cgctctcaat
    10141 agaccaagca tctaaagaat ccatttctgc cattactttc tctagacgtg attgcttact
    10201 ttcttcgtag tgattaagca ataattcata ttctttaatt aaagccatct ctcttaagtc
    10261 agaagaaagt acggtgtcca aaattgtctg agaatcatca aagtctggtt cttgttttaa
    10321 ataagcaatc ttataatcat tagctgatga aaaaggggaa cgatcaccat caaaacctaa
    10381 ttcacccgaa ataacatcta atagtgttgt ctttccagtt ccattgacac caataatccc
    10441 aatacggtct aaactatgga tgataaatga aacattacta aagaccgtct tatcaccaac
    10501 cgacttagtc aatccatcta ctaaaaaatc gctcatataa tcctctctaa tccatcaagt
    10561 actgcttaac aaaatgtaaa atagcctctt cttcattaat cagttgtcct aaaacaatgg
    10621 ctaattcaat ttgagagata atatctccca tctgtggacc aggttttatt cctaatttct
    10681 tgattaaggt gccaccattg acaactatct catgcctatc atggatagct aactcactat
    10741 ctagttgttc tattttttcg aaatcaacca tcaagcctct tgctttgcga ataccttccg
    10801 cttgttttat tatagttttt ccataacgat aaagatgttc tttagtcaat cccatttgtt
    10861 cacgaatacg ataaacatcc actatatgct caacatcttt ttgaaagtga gttgatgtct
    10921 tccatttttt taaaaaagtc tttgtgtgtt caacttccat actcaagatg agtgatgccc
    10981 aagcttgttc tgatgtctca aataaaaaat ctttatctaa ctgggacaaa aaggcgctta
    11041 attgtaattc cctatttttt aagcatggta gataattaaa agcatgacta tctattagtg
    11101 ccagcattcc tttacgccaa taaggtgcta atagaagttt atcaaattca ataaaagaac
    11161 gctcaacaga tattttctcc aataatggcg cgtgcttttt cattgcctca aaagttgttg
    11221 tttcgatatc aaaatttaga ctagcagaaa aacgcaaacc tcgcataatt cgaagagcat
    11281 cttcattaaa acgctcactg gcgagtccta ctgctctaag aaggtgatta tctaaatcat
    11341 ccaatccatg aaagagatca ataacttctc catcttcatt taaagcaaag gcattaactg
    11401 tgaaatctcg acgttttaga tcttcttcta gcgacctaac aaaattgacc tgactcggac
    11461 gcctataatc aacataaacc tcctcagtcc taaaagtggt aatctcatat tcaccacctt
    11521 tttccaatac taagactgtt ccatgttcaa taccaacatc aactgtcctt ttaaaaatct
    11581 gctttgtttc ttctggataa gatgacgttg ctatatcaac atcatgaatt ggacggtcaa
    11641 gtaaaacatc ccgaacacta ccacctacaa agtatgcttc ataaccggct ttttttatct
    11701 tttttaatat tggtaaagcc ttctgaaact cagaaggcaa ataatttaat ctcatcatac
    11761 tattccaaat ctaaacgaac taaagtcgca ttattatttc ttataaatgg attcagaaac
    11821 aaaaagaact gcctaaggga cactccctac ctactcaaat tatcaaaaaa gaatctccta
    11881 acttcttctt tgatatctgc aataaggatc aacctccaag caactctttt tatcacacat
    11941 caattttttg gggtaatacc aaaggcaacc gcaccttcac caagatgagt tgcaataacg
    12001 ccaccaaatg atacaatttc taaatcgtct ttaagaccag ctttagctaa caaattatat
    12061 aattgttctg ccttatcttg tgctctagag tgaataatag caatatcata ttcaccatct
    12121 gctgtcattt ctttgacaat ctcagccaag cgttttaagg cttttttctc tgtacgcact
    12181 ttctcataga caacgatttt accttcttca ttaaaatgta agaccggctt gatacttaaa
    12241 aggttaccaa tgatagctga accgtttgac aatctaccac cttttactag atgattcaaa
    12301 tcattaacaa caataaatcc ttcaattttt tcaatttgtg attgtatctt gttaactatg
    12361 acatcaaaat ccatcccttc acgtgaacac atcaaagcat ttctaactaa attgccctgt
    12421 ggtgctgaag ttattttagt atccggaaaa gcaattgtca aatttggatg ttcttcaata
    12481 agaaactgaa tattctgcca gaaacctgat atccccgctg caatgaataa accaaggacg
    12541 tgtgtatacc cttctttctc caattgacat aagagatcat ctagttcagc taagctaggc
    12601 tgacttgttt taggtaattc ttttgatgcc gctagcttat cataatattg gtctaatgtc
    12661 agattttgtc cctcaatata agttttacca tcaatgataa ttggaatatc taaaacatag
    12721 acatcttcat gattttcgat agcctcaggc aagtaagctg aggtatccgt aataagtgct
    12781 aatttcatta actattaaac tccaatgtta ctcctggtct atctaaagca atttctgtaa
    12841 cttcataagt cagacgttta acgatttcta aaagaggagc tgctagattc tcaacttcat
    12901 cttgactaaa ctcactaggc tcattgataa ggcgcccttg gatatgagct atttgtgaaa
    12961 tgacaccaga aataacaaac tcatcacgta caataacaaa ttgtaacact gctacaatgc
    13021 tcgttacttt tgctgcttca tctctattag ctaattggaa agtaacttca aaatttgttt
    13081 ttggggtacc attttcctct tcccattcta aattgcgcgc atcatagtgg tattgattga
    13141 caaattcttg ttcacgaatg acttccatgt attttctcct tgatagacaa tatgtattat
    13201 tatagcataa atttgtagat tcttaaagca ggtgggaatc tagaacttct tcttaggatg
    13261 tctatcaatc acttcttgat tgttactcaa ggctgctaaa cctttttctg taagtgccaa
    13321 ccctctgaca acaaacaact tccctaattc ctcttctgta aaagcaatag ctattgcctg
    13381 atctaaatca gagcgtttca tctttgacaa gccttttacg tttttttctt ttaagaatag
    13441 ttttaaaagt ggagcagata agtgatctaa agaatcaagc ggtgactcca ttcttacaaa
    13501 tccatttttt attaaaaatt ctaggtgttc ttttccattt ataccataaa tgtattcaaa
    13561 atattttgaa aaacttgttt cagttgtgaa agttccaaac tgtatacacc ataataagat
    13621 aatatctcca ggcaagagtc cttcactagt ttcttccata tttttcctag gaacaacttt
    13681 actaaaatta atttcagcca agtctctttc aggtgagata taggggatat taagataatt
    13741 tttgtaaatt tcttcaatag ttttcatagt catattgtag caaaaaagcg cttataaaaa
    13801 caagcgctaa ggcatggggg aagtcacctt tttgaaaaat tcaatttcat tttctgatat
    13861 tctaaccatg ttaaaattat catgataaca tcaaatacac atagaaaaat cattaaaatg
    13921 ctatgactag aaaaatattc ccttagttgg aaaatgataa acccaacgaa aactaaaatt
    13981 gataaagggt aagcaaataa tttctcttta tgtaataaaa taagtgctac ta
//
LOCUS       DATRUK010000057        13979 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.057, whole genome shotgun sequence.
ACCESSION   DATRUK010000057 DATRUK010000000
VERSION     DATRUK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13979
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.057"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            126..2690
                     /locus_tag="VBE34_001569"
     CDS             126..2690
                     /locus_tag="VBE34_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GBS Bsp-like repeat-containing protein"
                     /protein_id="HEO7264274.1"
                     /translation="MRPIVRKKMYKKGKFLVIAGIVTILGGSAILGQDVKAEQAEAVT
                     STISEKTDSSQTISDISKLTLPVNSSEAMKNSAEPLIKTGFATSVSSNPREIAATPVK
                     TFDASSKVVVKASTAEHSANQTNSNVNQVANDSEVITQQNSTKQLPTVTYSAHVQDIG
                     WQKSVDNATVSGTVGQEKQVEAIKLSIKAPEGITGKLSYKTYVKGQGWQPSVESGQVS
                     GTVGQSRPIEALSINLTDNLQKLYDVYYRVHVQDIGWMAWAKNGAYAGTLGMSKRLEA
                     YKVKFTLKGQSVLTPTILKEERPVLNYQVKVGQNGWQSNKLEGQMAGTLGESKALDGV
                     KFTLSTLKYGDILYRTHVQDKGWGEILGSQTSKDYQGKRLEAIQLDLSGNLKQNYDIY
                     YRAHVQDKGWMAWQKNNSVAGTVGESKRLEALEVKLVAKASDFSSQTNHSFLEMKHPG
                     LSYQTYLQKDGWKPTVLEGQLGGSIGLSKSIKAIKLNLGSTALGNIEYRTFLNGSGWQ
                     TVVNSGRESNVPNESQQVEAIQMRLTGLLSKTYSVMYRVHMQDYGWQDWTYNNDIAGS
                     TNQGKRIEAIEIKLVETAKMPITVQTTYKGTGNYNVRVTNVISPGNLKIAIWSDKNNQ
                     DDLKWYTVTPKNHEARLTFNVTNHRDNGKYFIHVYQENASNQKQLLVKTSLQVAHSNY
                     NTPYFSQRDGRWASRRYGMATLGETGCVPTSLAMILSSLKGETVLPTQIADYLYHKTV
                     EFNRGVQGTTSRGILKAAKEWGVTATALGTQANLVQALKDGYHVLAAVQNNVFVLHGS
                     HEIVLKGYNNGLTHVSDPYTPSLSGWYPISQLWKEQSYYSEDRIDIGAPFVKVTDA"
     gene            2813..3331
                     /locus_tag="VBE34_001570"
     CDS             2813..3331
                     /locus_tag="VBE34_001570"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEO7264275.1"
                     /translation="MDLNNYIASIENYPQEGITFRDISPLMADGKAYSYAVREIVQYA
                     ADKDIDMIVGPEARGFIVGCPVAYALGIGFAPVRKPGKLPREVISADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVVAGCAFLVELDGLNGRKAIE
                     GYDTKVLMNFPG"
     gene            3449..4129
                     /locus_tag="VBE34_001571"
     CDS             3449..4129
                     /locus_tag="VBE34_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEO7264276.1"
                     /translation="MTYLEQYQSGQLTLPSALFFHFKSIFKTADDFLVWQFFYLQNTT
                     NLSDLTPSRIATSLDKTVADINRSISNLTSQGLLDVKTIELNHEIEIIFDTSPVFAKL
                     DKLFEEDNQVIIDNKTADSNRLKDLVGDFERELGRLLSPFELEDLQKTLQEDQTDPDI
                     VRAALREAVFNGKTSWNYINAILRNWRREGLTTLRQIEERKQAREDNQMKDLAISDDF
                     KNAMNLWE"
     gene            4233..4916
                     /locus_tag="VBE34_001572"
     CDS             4233..4916
                     /locus_tag="VBE34_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEO7264277.1"
                     /translation="MDLQLSKRLQKVANYVPKGARLLDVGSDHAYLPIFLLQMGYCDF
                     AIAGEVVNGPYQSALKNVSEHGLTSKIDVRLANGLSAFEEADNIDTIAICGMGGRLIA
                     DILNNDIDKLQHVKTLVLQPNNREDDLRKWLAANDFEIVAEDILTENDKCYEILVVKH
                     GHMNLTAKELRFGPFLLSNNTTVFKEKWQNELNKLTFALNSIPNSKMEERAILEDKIQ
                     DIKEVLDES"
     gene            4906..5694
                     /locus_tag="VBE34_001573"
     CDS             4906..5694
                     /locus_tag="VBE34_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEO7264278.1"
                     /translation="MKARELIDVYETYCPQELSMEGDISGLQIGSLDKEIKTVMVALD
                     VRETTVAEAIERQVDLLIVKHAPIFRPLKDLVATPQNKIYIDLLKSDIAVYVSHTNID
                     IVPNGLNDWFCELLDIQYPDILSETSNGYGIGRIGDVRPQSFEFFAWKIKDVFGLDSV
                     RLVSYDKSNPEIQRVAICGGSGQSFYKEAIAKGADVFVTGDIYYHTAQEMITNGLLAI
                     DPGHHIEVLFVSKIATMIEQWKLEKGWDISVLESKAPTNPFYHM"
     gene            5703..6806
                     /locus_tag="VBE34_001574"
     CDS             5703..6806
                     /locus_tag="VBE34_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEO7264279.1"
                     /translation="MKKIAIIGAGAVGATLAYYLSKEKDIQVTVFDYGVGQATKAAAG
                     IISPWFSKRRNKAWYRMARLGADFYSKLVTDLQKDGFETKFYQQTGVFLLKKDESQLE
                     SLFALADKRRLESPLIGDLQILNKSEANTHFPELDGYEQLLYASGGARVEGADLTRIL
                     LEASGVNVIKDEVHFTITDNGFRVQGIDFDKLVLASGAWLAKILDEHNYQVDIRPQKG
                     QLRDYYFSNINTGKYPVVMPEGELDIIPFDNGKVSVGASHENDMAFDLNIDFKVLDKF
                     EEQAIGYFPQLKKADTTSERVGIRAYTSDFSPFFGPVPCMEGAYAASGLGSTGLTVGP
                     LIGYELCQLILNKENQLNLEDYDITKYVKKISR"
     gene            6865..7734
                     /gene="rfbA"
                     /locus_tag="VBE34_001575"
     CDS             6865..7734
                     /gene="rfbA"
                     /locus_tag="VBE34_001575"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEO7264280.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSVLMLA
                     GIKEILIISTPQDLPRFEDMLGDGSELGISLSYAEQPSPDGLAQAFIIGEEFIGDDHV
                     ALVLGDNIYHGPGLSAMLQRAASKESGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PAQPKSNYAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRMQNVQVANLEEIAYRMGYITREQVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            7734..8327
                     /locus_tag="VBE34_001576"
     CDS             7734..8327
                     /locus_tag="VBE34_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEO7264281.1"
                     /translation="MTEQFFDKELTCRPIEAIPGLLEFDITVRGDNRGWFKENFQKEK
                     MIPLGFPESFFEADKLQNNISFNKKNTLRGLHAEPWDKYVSIADEGRVIGTWVDLREG
                     DSFGNVYQTIIDASKGIFVPRGVANGFQVLSDKAAYTYLVNDYWALELKPKYAFVNYA
                     DPNLGIQWENLEEAEVSEADKNHPLLKDVKPLKKEDL"
     gene            8534..9580
                     /gene="rfbB"
                     /locus_tag="VBE34_001577"
     CDS             8534..9580
                     /gene="rfbB"
                     /locus_tag="VBE34_001577"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEO7264282.1"
                     /translation="MTEFKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGN
                     RANIEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLEDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGNGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLY
                     GEGKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKD
                     AYDHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKD
                     AVEAKYAKTQEVIK"
     gene            complement(9632..12850)
                     /gene="hylB"
                     /locus_tag="VBE34_001578"
     CDS             complement(9632..12850)
                     /gene="hylB"
                     /locus_tag="VBE34_001578"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_192884539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEO7264283.1"
                     /translation="MEIKKKHRIMLYSALILGTILVNNSYQAKAEELTKTTSTSQIRD
                     TQTNNIEVLQTESTTVKETSTTTTQQDLSNPTASTATATATHSTMKQVVDNQTQNKEL
                     VKNGDFNQTNPVSGSWSHTSAREWSAWIDKGNTADKSPIIQRTEQGQVSLSSDKGFRG
                     AVTQKVNIDPTKKYEVKFDIETSNKAGQAFLRIMEKKDNNTRLWLSEMTSGTTNKHTL
                     TKIYNPKLNVSEVTLELYYEKGTGFVTFDNISMKAKGPKDSEHPQPVTTQIEKSVNTA
                     LNKNYVFNKADYQYTLTNPSLGKIVGGILYPNATGSTTVKISDKSGKIIKEVPLSVTA
                     STEDNFTKLLDKWNDVTIGNYVYDTNDSNMQKLNQKLDETNAKNIEAIKLDSNRTFLW
                     KDLDNLNNSAQLTATYRRLEDLAKQITNPHSTIYKNEKAIRTVKESLAWLHQNFYNVN
                     KDIEGSANWWDFEIGVPRSITGTLSLMNNYFTDAEIKTYTDPIEHFVPDAEYFRKTLV
                     NPFKALGGNLVDMGRVKIIEGLLRKDNTIIEKTSHSLKNLFTTATKAEGFYADGSYID
                     HTNVAYTGAYGNVLIDGLTQLLPIIQETDYKISNQELDMVYKWINQSFLPLIVKGELM
                     DMSRGRSISREAASSHAAAVEVLRGFLRLANMSNEERNLDLKSTIKTIITSNKFYNVF
                     NNLKSYSDIANMNKLLNDSTVATKPLKSNLSTFNSMDRLAYYNAKKDFGFALSLHSKR
                     TLNYEGMNDENTRGWYTGDGMFYIYNSDQSHYSNHFWPTVNPYKMAGTTEKDAKREDT
                     TKEFMSKHSKDAKEKTGQVTGTSDFVGSVKLNDHFALAAMDFTNWDRTLTAQKGWVIL
                     NDKIVFLGSNIKNTNGIGNVSTTIDQRKDDSKTPYTTYVNGKTIDLKQASSQQFTDTK
                     SVFLESKEPGRNIGYIFFKNSTIDIERKEQTGTWNSINRTSKNTSIVSNPFITISQKH
                     DNKGDSYGYMMVPNIDRTSFDKLANSKEVELLENSSKQQVIYDKNSQTWAVIKHDNQE
                     SLINNQFKMNKAGLYLVQKVGNDYQNVYYQPQTMTKTDQLAI"
     gene            13067..13549
                     /locus_tag="VBE34_001579"
     CDS             13067..13549
                     /locus_tag="VBE34_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO7264284.1"
                     /translation="MTKLATICYIDNGKELLLLHRNKKENDVHEGKWISVGGKLEAGE
                     TPDECAKREILEETHLTVKKMDFKGVITFPEFTPGHDWYTYVFKVTDYEGELISDDES
                     REGTLEWVPYDQVLSKPTWQGDYEIFKWILEDVPFFSAKFVYDEHQNLIEKTVNFYEK
                     "
     gene            13539..>13979
                     /locus_tag="VBE34_001580"
     CDS             13539..>13979
                     /locus_tag="VBE34_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7264285.1"
                     /translation="MKNRDFRVRDQLETFGVRVSALIIENQKLLLIYAPHLYKYYLPG
                     GALQVGEDSNKAVAREVLEEIGLHSQVGDLAYIVENQFNIKRHHYHSVEFLYFVNLLG
                     QAPESIKEGTHKRHFVWLPIKELTKIDCNPNFLAQDLIEWPGHVV"
BASE COUNT         4520 a         2201 c         2832 g         4426 t
ORIGIN      
        1 agtggttttt ttggtatata aataagttat ttcaataata atattgcaaa tattattaaa
       61 atataataaa attaagaaaa taatggaatt tttgataaag aatattaaag gtgaggataa
      121 aatctatgag acctatcgtt agaaaaaaga tgtacaaaaa aggtaaattt ttggtcattg
      181 caggtatcgt tactatttta ggtggtagtg caattcttgg tcaagatgtc aaggcagaac
      241 aggcagaagc agtaacttca acgatctcag agaaaactga tagcagtcag actatttcgg
      301 atatttccaa gttaacgtta cctgttaatt cctcggaggc tatgaaaaat agtgcagagc
      361 ctttaataaa aacaggcttt gctacatctg tttcatctaa tcctagagag atagcagcta
      421 ccccagtcaa aacctttgat gctagttcaa aagttgttgt aaaagctagt actgctgagc
      481 actcagcaaa tcaaacaaat tctaatgtta atcaagtagc taatgatagt gaagttatta
      541 ctcaacaaaa ttcaacaaag caattaccaa cagtgactta ctctgctcat gttcaagata
      601 ttggatggca aaagtcggtt gataatgcaa cagtttcagg aacagtagga caggaaaagc
      661 aggttgaggc aataaaattg tctattaaag cccctgaagg aataacagga aaacttagct
      721 ataaaactta tgttaaagga caaggttggc aaccttcagt agagagtggt caagttagtg
      781 gtactgttgg tcagagtaga cctattgaag cactaagcat taaccttact gataatttac
      841 aaaaattata tgatgtctat tatcgtgttc atgttcaaga tatcgggtgg atggcttggg
      901 ctaaaaatgg agcttacgct gggactttgg gaatgtcaaa gcgccttgaa gcttacaaag
      961 taaagtttac ccttaaagga caatctgttt taacaccaac tatacttaag gaagagagac
     1021 ctgtcttaaa ttatcaggtt aaggtaggcc aaaatggttg gcaatctaat aaacttgaag
     1081 gacaaatggc gggaacttta ggagaatcca aagccctaga tggagtaaaa ttcactctta
     1141 gcaccttgaa gtatggggac attctctatc gtactcatgt acaagataaa ggttggggag
     1201 aaattttagg tagtcaaaca agtaaggatt atcaaggaaa aagactagaa gctattcaat
     1261 tggacttaag tgggaattta aaacaaaatt atgatattta ttatcgcgct cacgtccaag
     1321 ataaaggttg gatggcttgg caaaaaaata atagtgtagc aggtacagtc ggagaatcga
     1381 aacgtttaga ggctttggaa gtaaaactag tagctaaagc ttcagatttt agttcccaaa
     1441 caaatcattc attcttagaa atgaagcatc ctggattaag ttatcaaact tatcttcaaa
     1501 aagatggttg gaaaccaacg gttttggaag gccaattagg aggtagtatt ggactttcaa
     1561 aatctataaa agccatcaaa cttaacttag gtagtacagc tctaggaaat atagaatata
     1621 gaacttttct taatggttca ggttggcaaa ctgttgttaa ttcagggaga gaaagtaatg
     1681 taccaaatga atctcaacaa gtagaagcta ttcagatgcg attgacaggt ctgctatcga
     1741 aaacctatag tgtgatgtat cgagttcaca tgcaagatta tggttggcag gattggactt
     1801 ataacaatga catcgctggt agtactaacc aaggaaaacg aattgaagca atcgaaatta
     1861 aattagttga gactgctaaa atgcctataa ctgtccaaac gacctataag ggaactggaa
     1921 actataatgt acgagtgaca aatgttatta gtcctgggaa tttaaaaatt gctatttggt
     1981 cagataaaaa caatcaggat gaccttaagt ggtataccgt cacacccaaa aatcatgaag
     2041 ctagattgac atttaatgtt acaaatcata gagacaatgg taaatacttt atccatgttt
     2101 atcaggaaaa tgcttctaat caaaaacaat tattggtaaa aacttctcta caagtcgcac
     2161 attcaaatta taatactcct tactttagcc aacgtgatgg aaggtgggca agtcgaagat
     2221 atggtatggc aactttagga gaaactggtt gtgtaccaac ttctctagct atgattttgt
     2281 cttctttgaa aggagaaact gttttaccaa cgcaaattgc agattacctt taccataaga
     2341 cagttgaatt taatcgtggt gttcaaggaa caacttctag aggaatctta aaagcagcca
     2401 aagaatgggg tgtaacagca actgcattag gaactcaagc taaccttgta caagcgttga
     2461 aggatggtta tcatgtccta gcggcagtac aaaataatgt ttttgttctt catggaagtc
     2521 atgaaatcgt tctcaaagga tataataacg gactgacaca tgtctctgac ccttacacac
     2581 cgtccttgtc tggttggtat ccaatttcac aactttggaa agaacagagc tattatagtg
     2641 aggatagaat tgatattgga gcaccttttg tcaaggtaac agatgcctaa tttttaaaac
     2701 catatgcaaa actagcatat ggttttaaag tttccagtac tattttattt aattttatga
     2761 tataatgaag agtaaaacat tttaggggtt tccccttaga aagacaagtg aaatggattt
     2821 aaataattac atcgcttcaa ttgaaaacta tcctcaagaa ggaattactt tccgtgatat
     2881 ctccccatta atggctgatg gtaaagcata cagctatgct gttcgtgaaa ttgtacaata
     2941 tgctgctgat aaagatattg atatgattgt tggaccagaa gctcgtggct ttattgttgg
     3001 ttgcccagtt gcttatgctc ttggtattgg ttttgctcca gtccgtaaac caggtaaatt
     3061 accacgcgaa gttatttcag ctgactatga aaaagaatat ggtcttgata cattgacaat
     3121 gcacgcagat gctattaaac caggtcaacg tgttttaatt gttgatgacc ttttagctac
     3181 tggtggaaca gtaaaagcga caattgaaat gattgaaaaa cttggtggcg ttgtcgcagg
     3241 ctgtgctttc ctagttgaac ttgatggatt aaatggtcgt aaagctattg aaggttatga
     3301 tactaaggta ttgatgaact ttccaggata aggcctattc aagatagatg aatgataagg
     3361 gcgcctctaa gtgctatgat aatagttgag aggtgctttt tgttacacat ttcataattt
     3421 taggacgata gagaggattt gaaaggttat gacttatttg gaacaatatc agtcagggca
     3481 attgactcta ccaagtgctt tattttttca ctttaaatct atttttaaga cagcagacga
     3541 ttttttagtt tggcaatttt tctatttaca aaatacaact aatttatccg atctaacacc
     3601 gagtcgtatt gcgacatcac ttgataaaac agtggcagat atcaatcgtt ctatatcgaa
     3661 tttaacaagt caaggtttac tagatgtcaa aacaattgag ttgaatcacg aaatcgagat
     3721 tatctttgat acaagccctg tgtttgctaa attagataaa cttttcgaag aggataatca
     3781 agtaataata gacaataaaa ccgctgattc gaatcgtctg aaagatttag taggagactt
     3841 cgaacgtgaa ttaggtcgct tattaagtcc ttttgaatta gaagatttac aaaagacact
     3901 gcaagaagat cagacagatc ctgatattgt tagggcagct cttagagaag cggttttcaa
     3961 tgggaaaaca agttggaatt atatcaatgc tatccttcgc aactggcggc gtgaaggatt
     4021 gacgacactt cgacaaattg aagagagaaa acaagcacgt gaagataacc aaatgaaaga
     4081 tttagcgatt tcggatgatt ttaaaaatgc aatgaattta tgggaataag tttactagga
     4141 ggagacagaa tggttgatac attaggtctg ctccttttct tttgatgtta atgatgttat
     4201 acttttaaca gagaaaaact gaaggaaata caatggattt acaattatca aaacgattac
     4261 aaaaagttgc aaactatgtt ccaaaaggag ctcggttatt agatgtagga agtgatcatg
     4321 cttacttacc tatttttttg ttgcagatgg gatactgtga ttttgccatt gctggtgaag
     4381 tggtgaatgg accgtatcag tctgcgttga aaaacgtttc agaacatggt ctcacttcaa
     4441 agatagatgt tcgcttagca aatggtttgt cagcttttga agaagcagat aatattgata
     4501 cgatagctat ctgtggaatg ggtggtcgtt tgattgctga tattttgaat aacgatatag
     4561 acaaacttca gcacgttaaa acactagtct tacaacctaa taatcgggaa gatgatttaa
     4621 gaaaatggtt agctgcaaat gattttgaaa tagtagcaga agatatcttg actgaaaatg
     4681 acaaatgcta tgaaatttta gttgttaagc atggtcatat gaatttaact gctaaagaat
     4741 tgcgatttgg tccattttta ttatccaata acacaacagt ttttaaagaa aaatggcaaa
     4801 atgagcttaa taaattaact tttgctttaa actctattcc caatagtaaa atggaagaac
     4861 gtgctatttt ggaagataaa atacaggata taaaggaggt tcttgatgaa agctagagaa
     4921 cttattgatg tatatgaaac ttattgtcca caggaattat ctatggaagg agatatttca
     4981 ggtcttcaaa ttggatcttt agataaagaa ataaagacag tcatggtagc tttagatgtt
     5041 cgagagacta cagtagctga agcaattgaa cgtcaagtag atcttcttat tgtaaagcat
     5101 gctcctattt ttcgtccatt aaaggatttg gtagcaacac cgcaaaataa gatttatata
     5161 gaccttctta aatcagacat tgctgtttat gtgagccata ctaatataga tatcgttcct
     5221 aatggactta acgactggtt ttgtgaatta ttagatattc aatatcccga tatattatct
     5281 gagacaagta atggttatgg tattggtcgc attggtgatg ttagacccca gagttttgaa
     5341 ttttttgcat ggaaaattaa agatgtgttt ggtttagata gcgttcgttt agtttcatat
     5401 gataaatcta atcctgaaat tcaacgagtt gctatttgtg ggggtagtgg gcaaagtttc
     5461 tacaaagaag ctattgcaaa aggagcagat gtttttgtaa cgggagatat ttattatcat
     5521 actgcacaag aaatgatcac aaatggtttg cttgcgattg acccaggcca tcatattgag
     5581 gttttatttg tttctaaaat agctactatg attgagcagt ggaaacttga aaaaggctgg
     5641 gatatttcag ttttagagag taaagctcca acaaatccct tttaccatat gtaggagcta
     5701 ttatgaaaaa gatagcaatt attggtgcag gagcagtcgg agcgacacta gcctattact
     5761 tatcaaaaga aaaggatatt caggttactg tttttgatta tggagttgga caagcaacta
     5821 aggctgctgc ggggattatt agcccatggt tttcaaaacg tcgtaataaa gcctggtatc
     5881 gcatggcgag gttgggggct gatttttatt caaagctagt cacggattta caaaaagatg
     5941 gttttgaaac aaaattttat cagcaaactg gtgttttctt attaaaaaag gacgagagtc
     6001 agttagagtc attgtttgca ctagcagata aacgtcgact agaatctcct ctaataggtg
     6061 atttacaaat actaaataaa agtgaagcaa atactcactt tcctgagtta gatggctatg
     6121 aacagttact ttatgcgagt ggaggagcgc gagtagaagg ggcggactta acacgaatat
     6181 tgttagaagc aagtggtgtt aatgtcatca aggatgaagt tcactttacg ataactgata
     6241 atggctttcg tgttcaagga attgattttg ataaacttgt tttagcgagt ggagcctggc
     6301 tagctaagat attagatgag cacaactatc aggtagatat tcgccctcaa aaagggcaat
     6361 tgcgagacta ttattttagc aatattaata caggaaaata tcctgttgta atgccagaag
     6421 gcgagctaga tattatccca tttgataatg gtaaagtctc tgtaggtgca agtcacgaaa
     6481 atgatatggc ttttgattta aacattgatt ttaaagtctt agacaagttt gaagagcaag
     6541 ctattggcta ttttccgcaa ttaaaaaaag cagatacgac ttctgaacgt gttggaattc
     6601 gtgcctatac aagtgatttt tctccatttt ttggccctgt cccttgtatg gagggtgcat
     6661 atgctgcaag tggattagga tcaactggct taacagtggg cccattaatt ggttatgaat
     6721 tatgtcaatt aatcttaaat aaagaaaatc agctaaattt agaggattac gatattacga
     6781 aatacgtgaa aaagattagt agatgatagc gtttaaaaaa agtgttataa tatgtaggat
     6841 taatataaat aaggagacct tactatgaaa ggtattattt tagcgggagg ttcaggtact
     6901 cgcttgtacc cattaactcg tgctgcatca aaacaattga tgccaattta tgataaacca
     6961 atgatttatt atccattgtc agttttgatg ttggcaggaa taaaagaaat actgattatt
     7021 tctactccac aagatttgcc tcgctttgaa gatatgcttg gagatggttc agaactagga
     7081 atctctcttt cttatgctga acaaccaagc ccagatgggt tagcacaggc cttcattatc
     7141 ggtgaagaat ttattggcga tgatcacgtt gccttagtat taggtgataa tatttaccat
     7201 ggaccaggtt tatctgcaat gctacaacgt gcagctagta aagaaagtgg agcaaccgtc
     7261 tttggctatc aagtaaaaga tccagaacgt tttggtgttg tcgagtttga tactgatatg
     7321 aatgccattt ctattgaaga aaaaccagcg caacctaagt ctaattatgc tgtcacaggt
     7381 ctttatttct atgataacga tgtggttgaa atagctaaaa atattaagcc aagtcctcgt
     7441 ggagaacttg aaattactga tgttaacaaa gcctaccttg atagaggtga tttatcagtt
     7501 gagttaatgg gacgtggatt tgcgtggtta gacactggaa ctcacgaaag tcttcttgaa
     7561 gcagcacaat atattgaaac agtacaacgc atgcaaaacg ttcaagttgc taaccttgag
     7621 gaaattgctt accgcatggg ctacatcact cgtgaacaag tccttgagtt ggcacaacca
     7681 cttaagaaaa atgagtatgg tcaataccta cttcgtttga ttggagaagc ctaatgactg
     7741 aacaattttt tgataaagaa ttaacttgtc gcccaattga agccattcct ggattgttgg
     7801 aattcgatat taccgttcgt ggagacaacc gtggatggtt caaagagaac tttcaaaaag
     7861 aaaaaatgat accgcttggt ttcccagaaa gcttctttga ggcagacaaa ctacaaaata
     7921 atatttcgtt taacaaaaaa aatactttgc gaggtctcca tgcagagcct tgggataaat
     7981 atgtttcgat cgctgatgaa ggacgtgtga tcggtacttg ggttgacctc cgtgaaggtg
     8041 acagttttgg taacgtttac caaacgatta tcgatgcctc aaaaggtatt tttgttccac
     8101 gcggcgttgc taatggtttc caagttcttt cagataaagc agcttatact tatctcgtta
     8161 acgattattg ggcacttgaa ctcaaaccaa aatatgcttt cgttaactat gcagatccaa
     8221 atctaggcat tcagtgggaa aaccttgaag aagcagaagt ctcagaagca gataagaatc
     8281 acccacttct caaagatgta aaacctttga agaaggaaga tttgtaattt tcaacttctt
     8341 aaaaggcatt aaccttagca agtaaaaacg aattagcaat gttgtttgac gcaagggtga
     8401 aaggaaacat gcaaaaggcc tgttccctca gggatttgaa atattgattt cttaggcatg
     8461 acattccata gaactacttc taacagaaca gtatccttca aacagcaaaa agaaaaaatt
     8521 tttcggagaa aaaatgactg aatttaaaaa cattatcgta actggtggag ctggtttcat
     8581 cggatcaaac tttgtacact atgtttacaa taaccaccca gatgttcatg taactgtcct
     8641 tgataaattg acatatgcag gtaaccgtgc taacattgaa gccattcttg gtgatcgtgt
     8701 tgagttagtt gttggtgaca tcgctgacgc tgaattggta gacaaattgg ctgccaaagc
     8761 ggatgctatt gttcactatg cggctgagag tcacaacgat aactcattgg aagatccaag
     8821 tccatttatc catacaaact ttatcggaac ttacactttg cttgaagcag ctcgtaaata
     8881 cgatatccgt ttccaccacg tgtcaactga tgaagtttat ggagatcttc cacttcgtga
     8941 agaccttcca ggaaatggtg aaggaccagg tgaaaaattc actgctgaaa caaaatacaa
     9001 tccatcatca ccttactcat caactaaggc agcttctgac cttatcgtta aggcatgggt
     9061 acgttccttc ggcgtaaaag cgaccatttc gaactgttca aataactatg gaccatacca
     9121 acacatcgaa aaattcattc cacgtcaaat tacaaatatc ttggcaggaa tcaaaccaaa
     9181 actttatggt gaaggtaaaa acgtccgtga ctggatccac acaaatgacc attcaacagg
     9241 tgtctgggct attttgacta agggtcgtat tggtgaaaca taccttattg gtgccgacgg
     9301 tgagaaaaac aacaaggagg ttcttgaact tatccttgag aaaatgggtc aaccaaaaga
     9361 cgcttacgat cacgtaactg accgtgctgg tcatgatctt cgttacgcta tcgattctac
     9421 aaaattgcgt gaagaactcg gctgggaacc acaatttaca aacttttcag aaggtttgga
     9481 agaaactatt aaatggtata cagaaaatga gacatggtgg aaagcagaaa aagatgctgt
     9541 agaagccaag tatgctaaaa ctcaagaagt cattaagtaa ttgaaccaaa aaacctatca
     9601 ttttgatgat aggtttttta tgtacggcta gttagatagc taattggtct gtttttgtca
     9661 tggtttgagg ttggtaatag acattttgat agtcattacc aactttttgt actaggtaaa
     9721 gtcccgcttt attcattttg aattgattgt taatgagact ctcttgatta tcgtgtttga
     9781 taacagccca agtttgactg tttttatcat agataacttg ttgttttgaa ctattttcta
     9841 gtaattctac ttctttgctg ttggctaatt tatcaaaact tgtgcgatca atgtttggaa
     9901 ccatcatgta accatagcta tcacctttgt tgtcatgctt ttggcttata gtgataaaag
     9961 gattgctaac gattgaggta tttttagaag tacgattaat gctgttccaa gtacctgttt
    10021 gctctttgcg ttcaatatca atagtgctat ttttaaagaa gatataacca atattgcgac
    10081 caggttcttt tgattctaaa aagacacttt ttgtatctgt aaattgttga gaacttgctt
    10141 gttttaaatc aatagttttt ccattgacgt atgtagtata aggtgtttta gaatcgtctt
    10201 ttcgttgatc aattgttgta gaaacatttc caatgccgtt agtattcttg atgttgctac
    10261 ctaaaaagac aatcttatca tttaagataa cccaaccttt ttgtgctgtt aaggtgcgat
    10321 cccagttagt aaaatccata gcggcaagag caaagtgatc attaagtttg acggaaccaa
    10381 caaagtcaga tgttcctgta acttgaccgg ttttttcttt agcgtctttg ctatgtttgc
    10441 tcatgaattc cttagtggta tcttcacgct tagcatcttt ttcagttgtt ccagccattt
    10501 tataaggatt gacggttggc caaaaatgat tactataatg agattgatca ctattataaa
    10561 tatagaacat accatctccg gtataccaac cacgtgtatt ttcatcattc attccttcat
    10621 agttgagggt acgtttagaa tgtaatgaaa gcgcgaaacc aaagtctttc ttggcattat
    10681 aataagctaa gcggtccatg ctattaaagg ttgataaatt actttttaaa ggtttagtag
    10741 cgactgtact gtcattaagc agcttattca tgttggcaat atcggaatac gatttgaggt
    10801 tattgaagac attgtagaat ttatttgaag tgataatcgt tttaatagtt gatttgaggt
    10861 ctaagtttcg ctcttcatta gacatgttag ccaatctgag gaaacctctg agaacttcaa
    10921 ctgcagccgc atgcgaagaa gctgcctctc tactaattga gcgtccacga ctcatatcca
    10981 ttaactcacc ttttacaatt aaaggtaaaa atgattgatt aatccattta taaaccatat
    11041 caagttcttg attagagatt ttatagtcag tttcttgaat gataggcagc aattgtgtca
    11101 aaccatctat cagaacatta ccataggcgc cagtataagc aacatttgta tggtcgatgt
    11161 aagaaccgtc agcatagaag ccttcagctt tagtagcagt agtaaaaaga ttttttagag
    11221 aatgagaagt tttttcgata atagtattgt ctttacgaag taaaccttca atgattttaa
    11281 cgcgccccat atctactaga ttaccaccaa gggctttaaa tggattgaca agcgttttac
    11341 ggaaatattc tgcatcagga acaaagtgtt caattgggtc ggtataagtt tttatttcag
    11401 cgtcagtgaa gtagttattc atgagagata gggtacctgt aattgagcga ggaacaccga
    11461 tttcaaaatc ccaccaattg gcagagcctt ctatatcttt attaacattg tagaagtttt
    11521 gatgaagcca agccagactc tcttttacag tacgaatagc tttttcattt ttgtaaatag
    11581 tagagtgtgg attggtgatt tgtttagcta aatcttccaa acgacgataa gtagcggtta
    11641 actgtgctga attattgaga ttatctaaat ctttccaaag gaaagtgcga ttagaatcca
    11701 gtttgatagc ttcgatgttt ttggcgttag tttcatctaa tttctgatta agcttttgca
    11761 tgttcgaatc attagtatcg taaacataat taccaatagt cacgtcgttc catttgtcga
    11821 ggagttttgt aaagttatct tctgttgaag ctgtaactga taacggtact tctttaatta
    11881 ttttgccaga tttatcagat attttaacag ttgttgaacc agtagcgttt ggatacaata
    11941 ttccaccaac aattttccca agagacggat tggttagagt gtattggtag tcagctttat
    12001 taaaaacgta atttttgttt aaagccgtat taacgctttt ttcaatttgt gttgtgacgg
    12061 gttgtggatg ctctgagtct ttagggcctt ttgctttcat tgatatatta tcaaaagtaa
    12121 caaaaccagt tcctttttca taataaagtt caagtgtcac ctcggagaca tttaactttg
    12181 ggttatatat ctttgttaag gtatgtttgt tagtagtacc gctggtcatc tcagaaagcc
    12241 aaagtcgcgt attgttgtct tttttctcca taatacgaag gaaagcttgt ccagccttgt
    12301 tacttgtttc aatatcaaac ttaacctcat attttttagt gggatcaatg ttcacttttt
    12361 gtgttacagc acctctaaag cctttgtcgc tggatagact tacttggcct tgttcggtac
    12421 gttggataat aggtgattta tcagcagtat ttcctttatc aatccaagca gaccattccc
    12481 tagcgcttgt atgtgaccag cttccagata cagggttagt ttgattaaaa tctccgtttt
    12541 tcaccagctc cttattttga gtttgattat ctactacttg tttcattgtg ctatgagtgg
    12601 ctgttgcagt tgcagttgaa gctgtggggt tagacagatc ttgttgtgtg gttgtggtgc
    12661 tagtctcctt gacagtggta ctttcagtct ggagaacttc aatattatta gtttgagtat
    12721 ctcttatttg ggacgttgag gtagttttgg taagctcttc agctttagct tggtaactat
    12781 tgttaaccaa tattgttcct aaaataaggg ctgaataaag cataatacga tgtttctttt
    12841 tgatttccat ttttactcct tagggtttaa aattgtaaac cggtttttcc taattatagc
    12901 atgctttaaa ggtttatgat aaagaacctt taaaatgtca tcaaagtgaa aagtaaataa
    12961 ctggtaaaca aaataatttg tttaatcatt tgtgtgctac tttgatagta aattgcctaa
    13021 ataaaggcta ttttttgtta taatatgaga atgagaggag tttgaaatga ctaaattagc
    13081 aacaatttgt tacattgaca atggcaagga attacttcta ttgcatcgca acaaaaaaga
    13141 gaatgatgtt catgagggaa agtggatctc ggtaggcggt aaactggagg caggtgaaac
    13201 tccggatgaa tgtgctaagc gtgaaattct agaagaaacc catttaacag taaaaaaaat
    13261 ggattttaaa ggagtcatca cttttcctga atttacgcca ggtcatgatt ggtataccta
    13321 tgtctttaaa gtaacagatt atgaaggaga gttaatttca gatgatgaat cacgagaagg
    13381 aaccttagaa tgggtaccat atgatcaagt tctctctaag ccaacgtggc aaggagatta
    13441 cgaaattttt aaatggattt tagaagatgt tcctttcttt tctgctaagt ttgtttatga
    13501 tgaacaccaa aatttaattg aaaagacggt taatttttat gaaaaataga gattttaggg
    13561 ttagagacca gctcgaaaca ttcggagtca gagtttctgc tttaatcata gaaaatcaaa
    13621 aactgctatt aatatatgct cctcatctct ataaatacta tttgccaggt ggggcgcttc
    13681 aagttggaga agatagcaat aaggctgttg cgagagaagt acttgaggaa ataggtcttc
    13741 acagccaagt tggtgattta gcctatattg ttgagaatca gtttaatatt aagagacatc
    13801 attatcactc agttgaattt ttatattttg ttaatctctt gggtcaggcg ccagaatcta
    13861 tcaaagaagg aacgcataag cgacattttg tatggcttcc aataaaagaa ttaacaaaaa
    13921 ttgattgtaa tcctaatttt ttagcacagg atttgataga atggccaggt catgttgtg
//
LOCUS       DATRUK010000058        12781 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.058, whole genome shotgun sequence.
ACCESSION   DATRUK010000058 DATRUK010000000
VERSION     DATRUK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12781
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.058"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(60..643)
                     /locus_tag="VBE34_001581"
                     /pseudo
     CDS             complement(60..643)
                     /locus_tag="VBE34_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(750..2060)
                     /locus_tag="VBE34_001582"
     CDS             complement(750..2060)
                     /locus_tag="VBE34_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoFVII family restriction endonuclease"
                     /protein_id="HEO7264286.1"
                     /translation="MSIWERYSKEEREEYIKFLKVYGALTNLFRQKHENLIPYLDSKF
                     QETIYAKVFNSENVDIGNTPHDILSVFGDDRIGIGLKTWMNSRPSFQKVMQLKRYKAD
                     IDPYFNKSDEELALKLSELKNERMLIDYNRLGLAKDKNIYHYVTRDEGKFVIQETLYP
                     LVDLNKLGKFKRTNTAFTWSDGHKTYKYTYGDSQIWQYFNSDADDTLVLNQFDVNIIE
                     DPFDFLLKSYFSFVDDFKKAENSDDIVEVYLPLYSYRTKEVELKSGLNAWNAALKNKN
                     SATLRPLNEVYIPIPLEFHKKYPDFFCSDVFEIQRRQKNYVGNKKNKPQVRFHLQLPN
                     GKKIPALLTQSDMKSLQSGSLTEKDPNTGKLYGQSALGQWLLVDVLGLKERQPVTREW
                     LEKKGTDAVRLWHKKDDYNTINIDFAPIGSFETFMKDEINNSDD"
     gene            complement(2135..3355)
                     /locus_tag="VBE34_001583"
     CDS             complement(2135..3355)
                     /locus_tag="VBE34_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEO7264287.1"
                     /translation="MTSSVSSQQSKKYNLAGFFSGVGGIELGFTQTNEFRVVYANEFD
                     KYARMTYKLNYPDTNLDSRDIHAVDPKEIGQENIDIIVGGFPCQAFSIAGYRKGFEDD
                     RGDLFFELLRMIEDRKPRAIFIENVKNLVSHDHGNTFKVIREALTENNYFIKWKVLNG
                     KDYGNVPQKRERIYVVGFNNKTAYDLFEFPNEINLTTSLRDVIDFTENKDEIFYYREG
                     KQKFYSELEQVMTKQDTVYQWRRQYVRENKSGVVPTLTANMGTGGHNVPLILTDNGDI
                     RKLTPKETFNVQGYPKSFKVPENVSNGQLYKQAGNSVVVPVIKRIATNVAKALNNSTT
                     AKSEQSTGKVAIIYTKINGQFEGASYVKEFVDSYEHALARLESYQDNLPILSDEEYLK
                     IIKRKSKREFYSIV"
     gene            complement(3431..5065)
                     /locus_tag="VBE34_001584"
     CDS             complement(3431..5065)
                     /locus_tag="VBE34_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEO7264288.1"
                     /translation="MVVTKVLQIKTSRNLKRAIDYITRDNATLKLDTEHLEGDENYSY
                     EIVDGQVMKRLVSGHDLTDISDPQTIYEDFILLKQSVDVLYNNETLSDLKNDQRVLAH
                     HIIQSFSPEDGLTPEQVNEIGRKTALELTGGDYQFVVATHMDKGHLHNHIIFNTTNEV
                     TLKKFRWQKNTARNLFQISNKYAELYGAKVLEPRLRNSHTEYAAWRRKNNYRFEIKER
                     LNFLLKHSLDMNDFLQKAKALNLQIDTSGKYVKYMLMDQPQERFVRDRTLSKKGKFSL
                     EKIKEQIATNEVVYDLNVIKEKYDEEQESKQDDFELQVTIEPWQVQQLTSQSIYVPIT
                     FGLDRKGTVSIPARMLDQNEDGTFTAFVKKNDFFYFLNADHSEQNRFINGTTLIKQLS
                     AQNGEMILTKNKNITNLDRLVDEFNFLAINKVTNSKQFEELQTQFLKQLEETDKTLDV
                     LDEKMTYLNKLMGALSDYQNNIVPSEVSLELLETGKINKTTKLEELQKEIKELQIERD
                     TLKKHRDKIVKDYDFAKEMKEEHEKAKYKNSNKIKI"
     gene            complement(5037..5411)
                     /gene="mobC"
                     /locus_tag="VBE34_001585"
     CDS             complement(5037..5411)
                     /gene="mobC"
                     /locus_tag="VBE34_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEO7264289.1"
                     /translation="MADKKRKRSVEFKLRANEEEAAKIRQRFSRANKKTFQAYALEML
                     QNGKIATYDYSELQKLRIEVNRIGQNVNQLVRYVNTFDEIDNELFKALQAEIDDMKKL
                     LFDEFKTKGRAKLNGSDESSSD"
     gene            complement(5725..6018)
                     /locus_tag="VBE34_001586"
     CDS             complement(5725..6018)
                     /locus_tag="VBE34_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264290.1"
                     /translation="MITEEMMMITEMLLMSYYFDMSEWLKGVKTVNIDIIQKSKDDLL
                     DMLKSDFEELSTEDNNEYLDDLSVCIATLEELSEDAYQNIKTEVFSWEPGQKN"
     gene            complement(6076..10713)
                     /locus_tag="VBE34_001587"
     CDS             complement(6076..10713)
                     /locus_tag="VBE34_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEO7264291.1"
                     /translation="MANMQELLSQRFEARKQKLVSMDIVAVAESLGMKLKPGSSGAYY
                     WEEHDSFHIYPNTNSFCWWSRSVGSNTIDLVQVVREELTGHKPSFKEAAAFLETGHFE
                     NVTVQPPVREPFEYYLERYEHSDFNIGRQYLKEERGLSDDTINTFLASGNLASATRKK
                     GDYFEPVIVFKSRDNDGKMIGASLQGIVENRVQHSERGRLKQIMKNSDGTAGFSLDIG
                     NPKRLVFAEAPIDLMSYYELHKDELQDVKLVAMDGLKKSVISRYVADMLTDGKYSQTM
                     SNEQIRGALDALNKTTNILQEHPDLITLAVDNDEAGQNFIKGLQEDGIPVVSDLPPRQ
                     ANQIKMDWNDYLKQETNPKENIANKAWDIDDIQEQLSQESELVGTQTVDVVYMNEFYL
                     NQGYSLSLAVHSNEEVENLADDVAPWTLEVMKNGQSLGFLAYGEDWGNAFEIEAELAN
                     LEKWVKENQVSNHLYSQKDVNAFLATIQAVEPKRGEFVLKGGGIYDFGESQEEGEELR
                     QRALDGMIADITDGETYYLWYDEELENLNAPDEAFINFHYHLKDIEYNQDNIHLYVAD
                     STTDGVTGYLSLDGDSIEPTALEDYLTEQNWTFDQKVLFLKNLKTAIDETWDKVINHY
                     DEQFNAIVAQYGLSSAKEKAPELLQETEKANFEQQKKVENPIGDLPEQSQEAAPLPST
                     REEYSLNESSPTHSQSQPLLHFTISEPEKSIYKIGYHPVNKKELNKLNRYAAQIQENA
                     NWYKNELADSNVTYFYKNNDNLEALKVTFKAEQYPHLVGIFAIDDDQSASKTLDDLAS
                     GKGDYQNIMVANRGATFSKIQVLPDFKAVVDSNSFIFDDLSEVERMNRLDLAKAIRTE
                     DKDVLIAFRNVDGEYLPASLMKVTNKLESELSMANNKDVLGIIGEKDGEFKVLSVNEE
                     VIKDGGEKMLSIVKNGQLEDLSNTVQKAEWVVEFNEANLSGNVEIPNFTGAGLTEELL
                     ERIKDYDYRLYLASLNDEGEGYYKFYFNKVQDGKVVKQVRLDVGDGLERNEEIYKFLE
                     QELAPEKAVKVVEKELTAEDFTKVLDAVYNVGAQVGKDNRTNIPEELYPAWDKYYEYA
                     ERYDNNFDVIVKAARADNLFDENSDFYKDVWLRKIQEENQKVKDSDGDGLTDDEEIAL
                     GTNPYSPDTDGDGILDSVEKASGTDATNPSDTPDSRKEEMNKRDLTLSEMIKAKNTAA
                     LNQHLQEGIKDYFDSDNYKNFLEGMSHFNNYSVRNIQLIKAQLPEASMVASFNEWKKR
                     NGHVNKGEKALYVQAPVTVIKKDVDGNPIINSETGEKETLTYFKPVPVFDISQVSPVK
                     GKELNLPKMGEVIPSQITKEYYQNVYRSLRDISQKENGIPIRFRELEKSDGSYSRKTN
                     EITIKKGMTYEQTLSTLIHEMAHSELHNKKSLTERFEGKLTRSSKELQAESIAYVVSN
                     HLGFDTSNDSFAYLASWSKEPDGLENLKAQLEIVQEEASSLMKRIDKHLEKYQNLTVS
                     KDEKLNENQKRELEKTANPFYQSLQKAKGETVQTAPAKEEQSKKVNRPMKQ"
     gene            complement(10767..12503)
                     /locus_tag="VBE34_001588"
     CDS             complement(10767..12503)
                     /locus_tag="VBE34_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEO7264292.1"
                     /translation="MKYLLLAEKPDQAKKYANALGNPKNDKGVWRVQSSVLNAEVIVA
                     PAVGHLVERINPYTNFENWDMANLPALPDGFSYEPKKDTAKRFKAIKQAVKEVDAIII
                     GTDPDREGEAIAYRILELIPGALRKIKYRLWANSLTKKGLEQAFSKLKNPKDSINYFH
                     EADARGDADWLVGFNLSPFVTIKMKEEGYLDKKDHSMSVGRVQTPIVSLIVRNDEAIE
                     NFKPSPYWQLKLIDPEAKVTFSNDTKYQSKAEAEQMLQQLANIAVVKTVTNEEKVQEA
                     PKLYNLTNFQSEMSKLYHFDATKTKELVQSLYQKGYLSYPRTDSTLITTNEFAYLVEH
                     IDDYQIAIHRQLETPNRSPRKNYVNDKKVLEHYAIIPTENIPDLNELSDDEKLIYQKV
                     VFRTLLMVTPDYRYQSTSVILDNRGIEFKATGAVTKDKGWRNYLAVKKEDRELPDYQE
                     GKNLVVDCKLLEEMTKPPTRITEQILLKKLLPKYNLGTSATRDGMIDLIQDKGYVTKN
                     KKTGQFFPTERGKLLIHYLDQLEIAYTNPETTGKWEEILAQIGQGKIHKEAFVNKIKW
                     AITKQIEKGKQL"
BASE COUNT         3830 a         2411 c         1785 g         4755 t
ORIGIN      
        1 taattgtaaa tatatttata tatataatta taaatattat tgtatgtaat attgttatac
       61 tacctatgaa tgccatattt ttctcgataa cgtctaaacg ttcgttcatt aattcctgtc
      121 aattgtgcaa catctctatc tgactttcca gaaagaaaca agccaaaagc atgctgcagt
      181 ctgctgtcat cagctgaaaa cagtgcccta cgtcccttat atttaccctg ggcttttgca
      241 atggcaattc cttgtgcctg acgctcttta atacgttctc tttcagcttg cgcctgatac
      301 ttatataatt ccaatatcaa attatttaat agacgtctta gattatcatc ttcaatccct
      361 ctaagggtgg gagattaaga acttccaagg ttgcaccttt taattttatc tcatccataa
      421 cttcggttaa ttctttgtta tttcttccta gtcgctccaa ttcagttaca acgactatat
      481 caccatcacg aatataatca agcattgcaa tcaactgagg acgctctcta cttgctccac
      541 tgacagactc tttaaaaatc ttttcaacgt tatttaattg ttttaactgc ctatctaaat
      601 tttggtcagc tgaactgacc cttgcataac cgattttcac cattttttct cctctatttt
      661 gcctaactct attgtcctat tcaaaaatcc tttaaaatca aggtaaaaca aaaaggacaa
      721 taaggtatac ttatttgtcc tttacacaac taatcatcac tattgtttat ttcgtctttc
      781 ataaacgttt caaaacttcc aattggagca aaatcaatat taatagtatt ataatcatct
      841 ttcttatgcc acaatcgaac tgcatctgtt cctttcttct ctagccactc tcttgtaaca
      901 ggttgtcgtt cttttaaacc tagaacatca acaagaagcc attgaccaag cgcagattgt
      961 ccataaagtt tcccagtatt tggatctttt tctgttagac ttcctgattg caagctcttc
     1021 atgtcacttt gggtcaggag cgctggaatc tttttcccat ttggcaactg taaatggaac
     1081 ctaacttgtg gtttattctt tttgttacca acataattct tttgacgtct ttgaatctca
     1141 aaaacatctg aacaaaagaa atcagggtat ttcttgtgaa attctagagg aattggaata
     1201 tagacctcgt ttaggggtct taaggtggca ctgtttttat ttttaagtgc tgcattccaa
     1261 gcattaagtc cagattttag ttctacttct tttgttcgat aagaataaag aggcaaataa
     1321 acttctacaa tatcatctga attttctgct ttcttaaaat catcaacaaa actaaaatat
     1381 gatttcagta agaagtcaaa cggatcttca ataatattga catcaaattg atttaaaact
     1441 aatgtatcat cagcatcaga attaaaatat tgccatattt gagagtctcc atacgtgtac
     1501 ttataagttt tgtgaccatc actccacgta aaagcagtgt tagttctttt gaattttcct
     1561 agtttattta aatctacaag agggtacaag gtttcctgaa taacaaattt tccttcatcc
     1621 ctagtaacat aatgataaat atttttatct tttgctagac ctaatcgatt gtaatcaatt
     1681 agcattcttt cattttttaa ttcagaaagc ttaagcgcta gctcctcatc acttttattg
     1741 aagtaagggt caatatcagc tttataacgt tttaattgca tcactttttg aaaagatggt
     1801 cgtgaattca tccacgtttt taagccgata ccaattcggt catctccaaa cactgataaa
     1861 atatcatgtg gtgtatttcc aatatcaaca ttttcactat taaatacttt tgcgtagatt
     1921 gtttcttgaa attttgaatc tagataggga ataagatttt catgcttttg tctaaataaa
     1981 ttagtcaaag ctccataaac tttaaggaat ttgatatatt cttctctttc ctctttagaa
     2041 taacgttccc aaattgacat aataatccaa atacctttac ttaattttgt tcatattata
     2101 tcaaaatgaa gctagtttat cactagcttc atttttaaac tatactataa aattctcgtt
     2161 ttgattttcg tttgataatt tttaggtatt cttcatcact caaaataggt aagttatcct
     2221 gataagactc aagtcttgcc aaggcatgtt catagctatc cacaaattct ttaacgtagg
     2281 atgctccttc aaattgacca ttgattttag tataaataat agctactttt cctgtagatt
     2341 gctctgattt agctgttgtt gaattattta aagccttagc tacattagta gcaattcgtt
     2401 taataacggg tacaacaaca ctgtttcctg cctgcttata aagttgtccg tttgaaacat
     2461 tctctggaac tttaaaagat ttaggatatc cttgaacatt aaatgtttct ttaggtgtta
     2521 attttcgaat atctccatta tcagtaagaa ttaatggaac attgtgtcca cctgttccca
     2581 tgttggcagt caatgtggga acgacaccac ttttattttc tcgaacatac tgacgtctcc
     2641 attgataaac agtatcttgt tttgtcatca cctgctcaag ttcactataa aatttttgtt
     2701 taccttctct atagtagaat atctcgtctt tattttctgt aaaatcaata acgtctctca
     2761 aagaagttgt taaattaatt tcatttggaa actcaaataa atcataggct gttttattgt
     2821 taaaaccaac tacataaata cgttcacgtt tttgtggaac atttccataa tcttttccgt
     2881 ttaacacttt ccattttata aaataattgt tttcagttaa agcttctcgg ataactttaa
     2941 atgtatttcc atgatcgtgc gatactaaat ttttaacatt ttcaataaaa atagctctag
     3001 gttttctatc ttcaatcata cgaagtaact caaagaacaa atctcctcgg tcatcttcaa
     3061 aacctttgcg ataaccagca atactaaatg cttgacaagg aaagccaccc acaataatat
     3121 caatattttc ttgcccaatt tcttttgggt caacagcatg aatgtctcga ctgtccaagt
     3181 tagtatctgg atagtttagc ttatatgtca ttctggcata cttatcaaac tcattggcat
     3241 acaccactct gaactcgtta gtttgtgtga aacctaactc aattcctcca acaccactaa
     3301 aaaaaccagc taggttatat ttcttagatt gttgggaact tacagaactt gtcatgtata
     3361 ttcctcctat attaacaata actagtatag tataacacta aatataaaaa attgcagcca
     3421 agcaatcaac ttatatttta attttattgc tatttttata ttttgctttc tcatgctctt
     3481 ctttcatctc tttagcaaag tcataatctt ttacaatctt atctctgtgc tttttgagcg
     3541 tatcacgttc aatttgaagc tctttaattt ctttttgtag ctcttctagt tttgtggttt
     3601 tattaatttt accagtttca agaagttcaa gacttacttc agatggaaca atattatttt
     3661 gataatctga taaagctccc atcaatttat taagataagt catcttttcg tctaacacat
     3721 ctaaagtctt atctgtttct tccaattgtt tcaaaaactg agtttgtaac tcttcaaatt
     3781 gttttgagtt agtaaccttg ttaattgcca aaaagttaaa ttcatcaact aatctatcta
     3841 agttggtaat atttttattt ttagtcaaaa tcatttcacc attttgagca gatagttgct
     3901 tgattaaagt tgttccattg atgaaacgat tttgttcgga atggtcagca tttaaaaagt
     3961 aaaagaaatc attcttttta acaaaagctg taaatgttcc atcttcattt tggtcaagca
     4021 ttcttgctgg aatagaaacc gttccttttc gatctaagcc gaaagtaatt ggaacataaa
     4081 tagattgact agtaagttgc tgcacttgcc aaggttcaat tgtaacctgt aattcaaaat
     4141 catcttgttt gctttcctgt tcttcatcat atttttcttt tatgacattt aaatcataaa
     4201 caacttcgtt tgttgcaatt tgttctttga ttttttcaag tgaaaacttt cctttttttg
     4261 ataacgtacg atcacgaaca aaacgttctt gtggttgatc cattaacata tatttaacgt
     4321 acttaccact tgtatcaatt tgcaagttta aggcttttgc tttttgtaaa aagtcattca
     4381 tatctaaaga gtgttttaac aaaaagttca accgttcttt aatctcaaaa cgataattat
     4441 tttttcttct ccatgcagca tattctgtgt gggaatttct aagccttggt tctaaaactt
     4501 ttgcaccata aagctcagca tatttatttg aaatttgaaa taagtttcga gctgtatttt
     4561 tctgccatcg aaattttttt aaagtgactt cattcgttgt gttaaaaata atatggttgt
     4621 gtagatgacc tttatccata tgtgttgcaa ccacaaattg ataatcacca ccagttaatt
     4681 ctaaagcggt ctttcttcca atctcattaa cttgctcagg tgttaaacca tcttctggag
     4741 aaaaagactg aatgatatga tgagctaaaa ctcgttgatc atttttcaaa tcggataatg
     4801 tttcattatt gtacagaacg tcaacagatt gttttagtaa aataaaatct tcataaatcg
     4861 tttgcggatc agatatatct gttaaatcat gaccagatac caaacgtttc atcacttgac
     4921 catcaacaat ctcgtaagaa taattttcgt caccttccaa atgttcagta tctaatttta
     4981 aggttgcatt atcacgtgta atataatcaa ttgcacgctt taagttacga cttgttttaa
     5041 tctgaagaac tttcgtcact accatttaat ttagcacgtc ctttcgtttt aaattcatca
     5101 aaaagtaatt tcttcatgtc atcaatttca gcttgtaaag ctttaaatag ttcattatca
     5161 atctcatcaa aagtgttaac atagcgaact aattgattga cgttttgacc aatgcgatta
     5221 acttcaatcc ttaatttttg caattctgaa taatcataag ttgctatttt tccattttgt
     5281 aacatctcta aagcatatgc ttgaaacgtt tttttgttag cgcgagaaaa ccgttgacga
     5341 attttagcag cttcctcttc attagctctt aatttaaatt caactgaacg ttttcgtttt
     5401 ttatccgcca tgaactgatt tcctttctaa aaatctgata aaatggagct ttttgcccag
     5461 cccctagagg aggactactt ttactctggc aagattagca agtggtaccc cacttacgaa
     5521 aataaaaagc aaaaaagcct atttgtggga cacccccaca aacagacttt taacttttta
     5581 cactttgggg aaatccccaa tcccctattt ggcacttcgt gcatttttca aaaatgacaa
     5641 tttttgataa ctgcattttg ataaaatgca acaatagaaa aactgacaat tttcaaattg
     5701 tcagcattcc ttctcatgtc atttttagtt tttttgacct ggctcccagg agaaaacttc
     5761 tgtttttata ttttgataag catcttctga cagctcttct agtgtagcga tacaaacaga
     5821 aaggtcatcg agatactcat tattatcttc tgtagacaat tcttcaaaat cagattttaa
     5881 catatccaat aaatcatctt ttgatttttg gataatatca atattaactg ttttaactcc
     5941 tttgagccat tcactcatat caaaataata gctcattaat aacatttcag ttatcatcat
     6001 catttcttct gttatcataa cttatttctc cttccacttt taaagtcaat ccttttttat
     6061 gcgacctacg ctgcgttact gcttcatagg tcgattgact ttttttgatt gttcttcttt
     6121 agcaggtgcg gtttgaacag tttcaccttt tgctttttgt aaactttgat aaaatggatt
     6181 agctgttttt tctaattcac gtttttgatt ttcatttaat ttttcatctt ttgaaaccgt
     6241 tagattttga tatttttcta aatgcttgtc aatacgtttc ataagagagc ttgcttcttc
     6301 ctgaacaatt tcaagttgag cttttagatt ttctaaacca tctggttctt ttgaccaaga
     6361 agctagataa gcaaatgaat cattacttgt atcaaaacct aaatgattag atacaacata
     6421 agcaattgac tcagcctgta gttcttttga agaacgagtt aatttgcctt caaaacgttc
     6481 tgtcaaactc ttcttattat gtaattcaga atgtgccatt tcatggatta gtgttgaaag
     6541 agtttgctca taagtcatcc cttttttaat tgtgatttcg tttgtttttc ggctgtaaga
     6601 gccatctgat ttttcaagtt ctctaaaacg aattggaata ccattttctt tttgagaaat
     6661 atctcttaat gagcgataaa cattttgata atactcttta gtaatttggc ttggaattac
     6721 ttcacccatt ttaggtaaat tcaattcttt accttttaca ggtgatactt gactaatatc
     6781 aaaaacagga acaggtttaa agtatgtaag agtttctttt tctcccgttt cagaattaat
     6841 gatagggttg ccatcaacat ctttcttaat aacagtgact ggcgcttgaa cataaagggc
     6901 tttttcacct ttgttaacat gaccatttct tttcttccac tcattgaaag aagctaccat
     6961 acttgcttct ggtaattgag ctttgattaa ttgaatgttt cttacagaat agttattaaa
     7021 gtgtgacatt ccttcaagga agttcttata gttgtcacta tcaaaataat ctttaattcc
     7081 ttcttgtaaa tgttgattga gtgctgctgt atttttagct ttaatcattt ctgataatgt
     7141 taagtcacgt ttattcattt cttcttttcg actgtctggt gtatctgatg gattagtggc
     7201 atccgtacca ctagcttttt caacgctatc taaaatacca tctccatccg tatctggact
     7261 gtatggatta gtacctagtg caatttcttc atcatctgtt agaccatcac catcagaatc
     7321 ttttactttc tgattttctt cttgaatttt tcgaagccag acatctttat aaaaatcaga
     7381 attttcatca aagagattat ccgctcttgc tgctttaaca ataacatcaa aattgttatc
     7441 atatctttca gcgtattcat agtatttatc ccaagctgga tacaattctt ctggaatatt
     7501 agtcctgttg tctttaccaa cttgagcacc aacattgtaa acagcatcta aaactttagt
     7561 aaagtcttca gcagtcaatt ctttttcaac taccttaaca gctttttcag gtgctagctc
     7621 ttgttcaagg aatttgtaaa tttcttcatt tctttccaaa ccatcaccta catcaagacg
     7681 tacttgttta acgaccttac catcttgaac tttattaaag taaaacttat aatagccttc
     7741 tccttcatca tttagacttg ctaaatacaa acgataatca taatctttaa tacgttcaag
     7801 taattcttct gttagacctg cgcctgtaaa attaggaatt tctacattac ctgataaatt
     7861 agcttcatta aattcaacaa cccattcagc tttttggact gtatttgata agtcttcaag
     7921 ttgtccattt tttacaattg ataacatttt ttcaccacca tctttgataa cttcttcatt
     7981 aacagataag actttaaatt ctccgtcttt ctcgccaatg atacctaaaa catctttgtt
     8041 atttgccatt gataattcag attctagctt attagtaact ttcattaatg aagctggaag
     8101 atactcacca tcaacgtttc taaaagcaat caaaacatcc ttatcctctg ttcggatagc
     8161 ttttgctaag tctaggcgat tcatgcgttc tacttcagac aaatcatcaa aaataaagct
     8221 atttgagtca acaacagctt taaaatcagg taatacttga attttactaa aggtagcccc
     8281 tcgattagca accataatat tttgataatc acctttacca gatgctagat catctaacgt
     8341 tttacttgcg ctttggtcat catcgatagc aaaaatacca accaggtgtg gatattgttc
     8401 tgctttaaaa gttaccttta aagcttctag attgtcattg ttcttataaa aataagttac
     8461 attactatca gccaattcat ttttatacca atttgcattt tcttgaattt gtgctgcata
     8521 tcgatttaac ttatttaatt ctttcttgtt aactggatga taaccaatct tataaattga
     8581 cttttcaggt tctgaaatgg taaaatgaag caaaggttga gactgagaat gagtcggtga
     8641 cgactcattc aaagagtact cttcacgagt actaggtaga ggtgctgcct cctgactttg
     8701 ctcaggtaaa tccccgattg ggttctcaac ctttttttgt tgctcaaaat tagctttttc
     8761 tgtctcttga agtaattcag gagctttttc ttttgctgaa gataagccat attgagctac
     8821 aattgcatta aattgttcat catagtgatt aataacctta tcccaggttt catcaattgc
     8881 agtctttaaa tttttaagga acaatacttt ttgatcaaag gtccaatttt gttccgtcaa
     8941 ataatcttca agagcggtag gttcaatgga atctccgtct agtgaaagat atcccgtaac
     9001 tccatccgtt gttgagtctg ccacataaag atggatatta tcttgattat attcaatatc
     9061 ttttagatga taatgaaaat tgatgaaggc ttcatctggt gcgtttaaat tttctaattc
     9121 ttcatcgtac cataaatagt aagtttcacc atcagtaata tctgcaatca tcccatcaag
     9181 tgcacgttga cgtaattctt ctccttcttc ttgactttct ccaaaatcat agataccacc
     9241 acctttaaga acaaattcac ccctttttgg ttcaactgct tgtatagtag ctaaaaacgc
     9301 attaacatct ttttgactat atagatgatt agatacttgg ttttctttta cccatttttc
     9361 aagattggct aactcagctt caatttcaaa agcatttccc caatcttcac cataagcaag
     9421 aaaaccaagt gattgaccat tcttcataac ttcaagtgtc caaggtgcta catcatctgc
     9481 tagattttct acttcttcgt tgctatgaac agctaatgaa agactatacc cttgatttaa
     9541 ataaaactca ttcatataga caacatcaac ggtttgagtt cctactaact cactttcttg
     9601 tgacaactgt tcttgaatat catcaatatc ccaagcttta ttagcgatat tttctttagg
     9661 attagtttct tgtttgagat aatcattcca atccattttt atttgattag cttgacgtgg
     9721 tggtaaatct gatactacag ggataccatc ctcttgtaag cctttaataa agttttgacc
     9781 agcttcatca ttatcaaccg ctaaagtaat taaatctgga tgctcttgta aaatgtttgt
     9841 cgttttattt aaagcatcta aagcacctct aatttgctca tttgacatgg tttgagagta
     9901 tttaccatca gtcaacatat cagctacata acggctaatg acactctttt taagcccatc
     9961 catggctact aacttaacat cttgtagctc gtctttatgt aattcatagt aagacatcag
    10021 atcgattgga gcttcagcaa agactaaacg ttttggattt ccaatatcta agctgaaacc
    10081 tgcagtccca tctgaattct tcataatctg tttaagacgt ccacgttctg aatgctgcac
    10141 acgattttca acgatcccct gaagactagc gccaatcatc ttgccatcat tatcacgact
    10201 tttgaataca ataacaggtt caaaatagtc accttttttg cgtgttgctg atgcaagatt
    10261 ccccgatgct aaaaaagtgt taatcgtatc atcagataag ccacgttctt ctttcaaata
    10321 ttgtctacca atattaaaat cagaatgttc ataacgctct aaatagtatt caaatggttc
    10381 tcttacaggt ggttgaacag tgacattctc aaagtgacca gtctcaagaa aagctgcagc
    10441 ttccttaaaa cttggcttat gacctgtcaa ttcttctcgt accacttgaa ccaagtcgat
    10501 agtatttgag ccaacacttc ttgaccacca gcagaaagag tttgtattcg gatagatatg
    10561 aaaactatcg tgttcttccc aataataagc accgctcgaa cctggtttta atttcatccc
    10621 taaggattca gcaacagcta caatatccat actgactagc ttttgttttc gcgcttcaaa
    10681 gcgttgagaa agtaattctt gcatatttgc cataaatttc tcctttcaaa ccaaaaagtg
    10741 ccactatttt atataaacag tagcacttac aattgttttc ctttttcaat ttgttttgtg
    10801 atagcccact tgattttatt aacaaaggct tctttatgaa tttttccctg ccctatttgt
    10861 gccaatattt cttcccattt tcctgttgtt tcagggttag tataagcaat ctctagctga
    10921 tctagataat gaataagtaa ttttcctctt tctgtcggga aaaactgtcc tgttttctta
    10981 ttttttgtga cataaccctt atcttgaatt aaatcaatca tcccatcacg agttgcggat
    11041 gtacctaaat tatattttgg taacaatttc tttaatagaa tttgttctgt aattcgtgtt
    11101 ggcggctttg tcatttcctc taagagtttg caatcaacaa caagattttt cccttcctgg
    11161 taatctggta gctccctatc ttcttttttt accgctaaat agttacgcca tcccttatcc
    11221 ttagttacag caccagttgc tttaaattca atcccacgat tatcgagaat aacactggtt
    11281 gattgatagc gatagtcagg ggtaaccatc agtaaggttc tgaaaactac cttttgataa
    11341 atgagttttt catcatcact tagctcattt aaatcaggaa tattttcagt tggaataata
    11401 gcatagtgtt ctagaacttt cttatcattc acataatttt ttcttggtga gcgattaggt
    11461 gtttctaatt gcctatggat tgctatttga taatcatcta tatgttcaac caaataagca
    11521 aattcattgg ttgtaatcaa ggttgagtct gtacgtggat aagaaagata tcctttttga
    11581 taaagacttt gaaccaattc tttggtttta gtcgcatcaa aatggtatag cttactcatt
    11641 tctgattgaa aattagttaa attataaagc ttaggtgctt cttgcacttt ttcttcattc
    11701 gtaaccgttt tgacaactgc tatattagct aactgttgta gcatctgttc tgcttcagct
    11761 tttgattgat atttagtatc attgctaaat gtaactttag cttcaggatc aatcagtttt
    11821 agctgccaat aaggactagg tttgaaattt tcaatggctt catcatttcg aacgattaga
    11881 ctaacaatag gtgtttgaac tcgtccaaca gacatagaat gatctttctt gtctaaatac
    11941 ccttcttctt tcattttaat ggttacaaaa ggacttagat taaaaccaac caaccaatca
    12001 gcatcacctc ttgcatcagc ttcatgaaaa tagttgatgg agtcttttgg atttttaagc
    12061 tttgaaaaag cttgctctaa accttttttc gttaaagagt ttgcccacaa gcgatattta
    12121 atttttctta gcgcacctgg tattaattct aaaattcgat aagcaatggc ttctccttca
    12181 cggtcagggt ctgttccaat aatgatggca tcaacttctt taacagcttg tttgatagcc
    12241 ttaaatcgtt tggcagtatc ttttttaggc tcataagaaa atccatctgg taaagctgga
    12301 agattagcca tatcccaatt ttcaaagtta gtatatggat taatacgttc tactaaatga
    12361 ccaacggcag gagcaacaat cacttcagca ttcaatacag atgactgaac acgccaaaca
    12421 cctttatcgt ttttagggtt tcctaaagcg ttcgcatact ttttagcctg gtcaggcttt
    12481 tctgctaaaa gtaaatattt caataaaatc actctccttt tcattatttc taccatagtt
    12541 ataaaatatt ttttattaag aaaataaatg ataaaataga ctaaaaatta tttagaggta
    12601 accaatgctt tctgatctta ttttatttat taatatgtac acgaagtcct atctttcttt
    12661 taaaagcaat aaaatactga ttgattacca acaacgttgg ataaaattat tttctaaagt
    12721 aatttcaaat cctttaccag aagaactaaa aatacgattc aatgaatgct tttcacttcc
    12781 t
//
LOCUS       DATRUK010000059        12741 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.059, whole genome shotgun sequence.
ACCESSION   DATRUK010000059 DATRUK010000000
VERSION     DATRUK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12741
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.059"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..452)
                     /locus_tag="VBE34_001589"
     CDS             complement(<1..452)
                     /locus_tag="VBE34_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264293.1"
                     /translation="MIRHSFKSKMLLLVFILTIIVNYLSATGLLTGNSQKSLSDRYQT
                     LLTPAPLAFSIWSVIYLLTFLVILRAIFSKSQSYQDNFASIFPYFLGLLLVNNIWTVF
                     FTSNLIGLSTIIIFAYCILLVIIIKILSKNKSKLLLRITFGIHAGWLLV"
     gene            complement(576..788)
                     /locus_tag="VBE34_001590"
     CDS             complement(576..788)
                     /locus_tag="VBE34_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEO7264294.1"
                     /translation="MSEFTFEIVEKLLVLSENEKGWTKELNRVSFNGAPAKFDLRTWS
                     PDHTKMGKGITLSNEEFKVILDAFRK"
     gene            complement(915..2201)
                     /locus_tag="VBE34_001591"
     CDS             complement(915..2201)
                     /locus_tag="VBE34_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO7264295.1"
                     /translation="MSNVKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGINYAHARDAKVYVAANMVTHEGNELGAGPWFRELRDMGLDAVIVSDP
                     ALIVICATEAPGLEIHLSTQASSTNYETFEFWKEMGLTRVVLAREVTMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGQERQSL
                     KGEIPEPFSMSAVDMCMIEHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFEAIKEDLIDELWKVAQRELATGFYYHTPTENEQLFGARRKIPQYKFVGEVV
                     SFDNAKMEATIRQRNVIMEGDRVEFYGSGFRHFECFIEGLRDAEGNKIDRAPNPMELL
                     TITLPNPVKKGDMIRACKEGLVNLYQNDGTSKTIRA"
     gene            complement(2331..3257)
                     /locus_tag="VBE34_001592"
     CDS             complement(2331..3257)
                     /locus_tag="VBE34_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO7264296.1"
                     /translation="MEKIILTATAESIEQVKQLLAIGIDRIYVGEENYGLRLPHSFSD
                     DELREIAKLVHDAGKELTVACNALMHQEMMDNIKPFLELMKEINVDYLVVGDAGVFYI
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVETVLAREIPSEELFKMSENLEFPAE
                     ILVYGASVIHHSKRPLLQNYYNFTHITDEKTRERGLFLAEPGDPESHYSIYEDKHGTH
                     IFINNDINMMTKVTELVEHHFTHWKLDGIYCPGDNFVAIAEIFVETARLIENGTFTQD
                     QAFLFDERIRKLHPKGRGLDTGFYDFDPSTVK"
     gene            3602..3877
                     /locus_tag="VBE34_001593"
     CDS             3602..3877
                     /locus_tag="VBE34_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEO7264297.1"
                     /translation="MSSPLKQEQTHESKTEQAQPKFQVFQEFDHSAKLRELVFFARIA
                     LFSITTVLISFLLLALSLTPVWSFLLAMLASLAVTSAVSSFIWSLRQ"
     gene            complement(3918..4349)
                     /locus_tag="VBE34_001594"
     CDS             complement(3918..4349)
                     /locus_tag="VBE34_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO7264298.1"
                     /translation="MSKLFKTLVISAASGAAAAYFLTTKKGKELRKNAEKFYGEYKEN
                     PEEYHQIAKDKASEYSNLAVDTFKDYKGKFESGELTTEDIVSAVKEKSGEVVDFANDF
                     VNQAKSKFSDEDTAKKEDKAPETKVEDIVIDYKENTEDKEK"
     gene            complement(4346..4744)
                     /locus_tag="VBE34_001595"
     CDS             complement(4346..4744)
                     /locus_tag="VBE34_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEO7264299.1"
                     /translation="MIEIAVLIIAIAFVVLVLGILFVLKKVSETIEETKQTIKVLTSD
                     VNVTLYQTNEILAKANVLVDDVNGKVSTIDPLFVAIADLSESVSDLNLQARHIGQKAS
                     SATSSVTKAGSALAIGKAASKIFRKKGDKK"
     gene            complement(4759..5532)
                     /locus_tag="VBE34_001596"
     CDS             complement(4759..5532)
                     /locus_tag="VBE34_001596"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEO7264300.1"
                     /translation="MINPVAIQLGPFSIRWYAICIVSGMLLAVYLAMKEAPRKNIKSD
                     DILDFILMAFPLSIVGARIYYVIFEWAYYSKHPVEIIAIWNGGIAIYGGLITGAILLV
                     IFSYRRLINPIDFLDIAAPGVMIAQAIGRWGNFINQEAYGRAVKNLNYLPNFIKNQMY
                     IDGAYRVPTFLYESLWNFLGFVIIMSIRHRPRTLKQGEVACFYLVWYGCGRFIIEGMR
                     TDSLYLAGLRVSQWLSVILVIIGIVMIIYHRREQHISYY"
     gene            complement(5525..6460)
                     /locus_tag="VBE34_001597"
     CDS             complement(5525..6460)
                     /locus_tag="VBE34_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEO7264301.1"
                     /translation="MAVTVQMLVDRLKLNVIYGDEHLLSKRITTADISRPGLEMTGYF
                     DYYAPERLQLVGMKEWSYLMAMTGHNRYQVLREMFQKETPAIVVARDLEIPEEMYEAA
                     KDTGIAILQSKAPTSRLSGEVSWYLDSCLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDIMSLYGAS
                     AVKDSSQVQLAIYLENFETGKVFDRLGNGNEEIELSGVKVPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKQMGFDATQTFEDRLTHLISQNEVNDD"
     gene            complement(6688..6951)
                     /locus_tag="VBE34_001598"
     CDS             complement(6688..6951)
                     /locus_tag="VBE34_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEO7264302.1"
                     /translation="MKSSFYKQRKGKLVCGVVAGLADKYNWDLALSRVLIALILYFTK
                     FGLLLYILLAVFLPYKEDIIETRRQGPRRRKDAEPVDDDGWFW"
     gene            complement(7032..7490)
                     /locus_tag="VBE34_001599"
     CDS             complement(7032..7490)
                     /locus_tag="VBE34_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEO7264303.1"
                     /translation="MDLVLSSQTDLTNYVKKVSIEDFGCQFLHTAHWNNRLRTTGGRF
                     FPNDRHLDFNPKIYREFGIDIFRKIVRHELCHYHLYIQGKGYRHRDKAFKELLEKVDG
                     LRYTPRVTAVKVNYHHYSCQSCGQVYRRRRRVNLRKFACGYCHGRLIESF"
     gene            complement(7453..9615)
                     /locus_tag="VBE34_001600"
     CDS             complement(7453..9615)
                     /locus_tag="VBE34_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEO7264304.1"
                     /translation="MENLNITQIAIDLGIKASQIEKVLELTDEGNTIPFIARYRKEMT
                     GNLDEVQIKSIIDLDKSMTALSDRKTTVLAKIEEQGKLTQELKKAIEEATKLADVEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNKDNLEEEAQNYLTDGFETTTKALSGAVD
                     ILIEAFSEDNKLRSWTYNEIWNYSSITAVVKDESLDEKQVFKIYYDFSEKISKLHGYQ
                     VLALNRGEKMGVLKVNFEHNLEKMFRFFAVRFKETSQYIDDLIVQTVKKKIVPAMERR
                     IRTELSEGAEDGAISLFSENLRNLLLVSPLKGKMVLGFDPAFRTGAKLAVVDQTGKLM
                     TTQVIYPVPPANQAKIEQSKIELAKLIKEFNIEIIAIGNGTASRESEAFVAEVLQDFP
                     DVSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLAENLDFVVETVVNQVGVNVNTASPALLAHVSGLNKTISENIVKYR
                     EENGQIKSRAEIKKVPRLGAKAFEQAAGFLRIPNAKNFLDNTGVHPESYEAVKKLLDQ
                     LTIKELDDLAKEKLQHLDLIATAESIGVGQETLKDIIEDLLKPGRDLRDDFEAPVLRH
                     DVLDVSDLKVGQELQGTVRNVVDFGAFVDIGVHEDGLIHQSRLIKRKRDKKTRKMPPL
                     PHPSKYLSVGDIVTVWVVEVDAERGRIGLSLIKPDGLD"
     gene            complement(9719..10762)
                     /locus_tag="VBE34_001601"
     CDS             complement(9719..10762)
                     /locus_tag="VBE34_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEO7264305.1"
                     /translation="MIELGITTFGETTILEETNQSYSHPERIRQLVAEIELADQVGLD
                     VYGIGEHHREDFAVSAPEIILAAGAVRTNNIRLSSAVTILSSNDPIRVYQQFSTIDAL
                     SNGRAEIMAGRGSFIESFPLFGYDLADYDDLFNEKMDMLLAINSATNLDWKGHLTQTV
                     NERPIYPRALQRQLSIWVATGGNVDSTIRIAEQGLPIVYATIGGNPKAFRQLVHIYKE
                     VGSRNGHKPEQLKVAAHSWGWIEEDNQTAIDRYFFPTKQTVDNIAKGRPHWSEMTKEQ
                     YLRSVGPEGAIFVGSPEVVAHKIIGLVEALELDRFMLHLPVGSMPHKDVLNAIKLYGE
                     EVAPIVRKYFEKN"
     gene            10895..11023
                     /locus_tag="VBE34_001602"
     CDS             10895..11023
                     /locus_tag="VBE34_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7264306.1"
                     /translation="MAITHKRQDDLESMFASFAKVPKPKKVDSDSKPEQKDDSQKD"
     gene            11029..11931
                     /locus_tag="VBE34_001603"
     CDS             11029..11931
                     /locus_tag="VBE34_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO7264307.1"
                     /translation="MDIFNQLPDSVLQWFAIFISIIIEALPFVLLGTILSGIIEVFIT
                     PDIVNKFLPKNKFLRVLFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSIRFLILRFVGATIVAIALGVMLAFLVDDNILKEDAKPTHFH
                     DYSDKKWYQKIFLALAHAIDEFFDTGRYLVFGTLIASAMQIYLPTRVLTTIGHSPITA
                     ILVMMLLAFILSLCSEADAFIGASLLSTFGIAPVMAFLLIGPMIDIKNLMMMVNSFKT
                     RFIVQFISVSSLIIIIYCLFVGVI"
     gene            11931..>12741
                     /locus_tag="VBE34_001604"
     CDS             11931..>12741
                     /locus_tag="VBE34_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEO7264308.1"
                     /translation="MIRFLILAGYFELSMYLKLSGKLNQYINTHYTYLAYISMVLSFI
                     LAIVQLIIWVKNMKMHSHLHGKIAKSTSPMILVFPVLVGLLVPTVSLDSTTVSAKGYN
                     FPLAAGSTGTVSQDGTRVQYLKPDTSTYFTSSAYEKEMQKELKKYKGSGTLTITTENY
                     MEVMELIYLYPEQFMDRQIQYTGFVYNEPKHEGYQFIFRFGIIHCIADSGVYGLLTTG
                     NQKSYPDNTWVTVRGTIKSEYNQLLQQNLPVLHIEESRQVSKPNNPYVYRVF"
BASE COUNT         4019 a         2541 c         1933 g         4248 t
ORIGIN      
        1 actagcaacc atcctgcatg aattccaaat gttattctaa gcagtaattt tgatttattt
       61 ttacttagta tcttgataat gataaccaga agaatacagt aagcaaaaat tattattgtt
      121 gataggccaa tcaaattgct cgtaaaaaat acagtccata tattattaac taatagaaga
      181 cccaaaaagt atggaaaaat gctagcaaag ttatcttggt aagattgact cttactgaat
      241 atagctctta atattactaa aaaggtcaat aaatagatga ctgaccaaat tgaaaaggct
      301 aatggtgctg gtgtcaagag cgtttgataa cgatcagaca gactcttttg actgttccct
      361 gttaacaaac ctgttgcgga taaataattc actattattg ttaagataaa gactaacaat
      421 aacatcttac ttttaaatga atgtctaatc atagaggact ccttttacct ccattataat
      481 tcttttagtt taaaaattct atttttttaa tcaacatgtt tactgaatac tcgcttgtct
      541 cgacaaaaaa agtggttttt gaaaccactt tttatttatt tacgaaaagc atctaaaatg
      601 actttaaatt cttcattaga taaagtgata cctttaccca tttttgtatg atctggactc
      661 catgtacgca aatcaaattt agctggcgct ccgttgaaac taacacgatt caactctttt
      721 gtccaacctt tttcattttc agataggacc aataacttct caacaatttc aaatgtaaat
      781 tctgacattt tatacctcca cttatactat tcgttatttt tatagctttt tgttctattt
      841 aaatgaactt cttctatttt aacaattttt agtaatattg accatgtaaa atgacaaaat
      901 agttataaaa gtatctaagc tcttatagtc ttgctagtac catcattttg ataaaggttc
      961 actaatcctt ctttacaagc acgaatcata tccccttttt ttactggatt tggtaatgtt
     1021 atggttaata attccatcgg atttggagca cggtctattt tatttccttc agcatcgcgc
     1081 agaccttcaa taaaacattc aaagtgacgg aagccagaac catagaattc tacgcgatct
     1141 ccttccataa taacattacg ctgacgaatt gtagcctcca ttttagcatt gtcaaatgaa
     1201 accacttccc caacaaattt gtattgagga attttacgac gagctccaaa gagttgttcg
     1261 ttttcagttg gtgtatggta gtaaaaacct gttgctaatt cgcgttgtgc aaccttccaa
     1321 agttcatcaa tcaagtcttc tttaatagct tcaaaagctt ctggactttc catataggca
     1381 tctacagcag ctttatagca attagttact gtagaaacat aatgaatgga tttcatacgt
     1441 ccttctattt ttaaactatc tacgccattt tcaatcatat ctggaatatg ctcaatcata
     1501 cacatatcca cagctgacat tgagaaaggt tctggaatct cgccttttaa cgattgacgt
     1561 tcttgtccaa atggcatatc gtagaggtca tatttccaac gacatgactg agagcaaccg
     1621 ccacgattag catcacgatg gctcatatgg tttgatagaa cacatcgtcc tgagtatgaa
     1681 atacacatcg cgccatgaac aaatgcttct atctcaacat ctgtcctctt cctgatttca
     1741 gccaactctg ccatagtaac ctcacgtgct aatacgacac gagtaagccc catctctttc
     1801 caaaattcaa aagtttcata gttcgtggaa gaggcttgag ttgacaaatg aatttctaaa
     1861 cctggtgctt ctgtagcaca aataacaata agagcaggat ctgaaacaat gactgcatct
     1921 agtcccatat cacgtaattc acgaaaccac ggacctgccc caagctcatt accttcatga
     1981 gtaaccatat tagccgctac ataaacttta gcatctctcg catgagcata gtttattccc
     2041 tcttgtaact cttccataga aaagttacca gctctacttc gaagaccata cgcttgacct
     2101 ccaacaaata cagcatctgc tccataatca atagcaactt ttaatttttc tagtgttcct
     2161 gcaggtgata aaacctcagg gcgttttttt acattagaca tcttatattt ccttctattt
     2221 tcaagataaa agaattattt tctatcttaa ttaacatacc aatcgatatg cagttcttag
     2281 tgcaattggc tacgctttga gataaaaaac tttcacgtag caaataaatc ttattttact
     2341 gttgatgggt caaaatcata aaatccagta tctaaaccac gtcctttagg gtgcagtttt
     2401 ctaattcgtt catcaaacaa gaaagcctga tcctgagtga atgttccatt ttcaatcaaa
     2461 cgagctgttt cgacaaaaat ttcagcaata gcaacaaaat tatctcctgg acaataaata
     2521 ccatctaatt tccagtgagt aaagtgatgt tcaactagtt cagtcacttt agtcatcata
     2581 ttgatatcat tgttaataaa aatatgagtc ccatgcttat cttcgtaaat agaatagtga
     2641 ctttctggat cacctggttc tgctaaaaaa agaccacgct cacgtgtttt ttcatcagtg
     2701 atatgagtaa aattataata gttttgcaat aacggacgct tagagtgatg aattacagaa
     2761 gccccataaa ctaaaatctc agcaggaaat tcaaggttct cagacatctt aaaaagttct
     2821 tcagaaggta tttcacgtgc taaaaccgtt tctactgctc catgttgtcc ccaaaaatta
     2881 acttgacgac ttgatgtaac gaatacagaa gtgtcgtaga taagtttgaa gttataacca
     2941 tcacgcttgt tgatataaaa tactcctgca tcaccaacta ccaaataatc cacattaatt
     3001 tctttcatca actccaaaaa tggtttgata ttatccatca tttcttgatg catgagtgca
     3061 ttacaagcta ctgtcaattc tttaccagca tcatgtacca atttagcgat ttcacgcaat
     3121 tcatcgtctg aaaatgaatg tggtaaacga agaccgtaat tttcttcccc tacgtagatg
     3181 cggtcaattc ctattgctag caattgcttt acttgttcaa tagattcagc tgtcgctgtc
     3241 aaaattattt tttccataat tagtattata acaaactttc agcattttta agcatatttc
     3301 ttgacaagaa taaataaaaa atattctttt cgaaaattat atcactttag aggttaagca
     3361 ctatcagata agaaaaaaaa acatagtatt gaaatactat acgaaattat atctattttt
     3421 ttcctttcca atttaaaaaa gtaaggaaag tagtaaaaaa tacttttagt attctactac
     3481 taatatgggc aatgtttttg tttttttgag aaaaaacctt tattttaact catagtatca
     3541 aaaaactagt gacaaactct attatttgtg atatgatatg ctagtatcaa aggagaaagc
     3601 tatgtcatca ccacttaagc aagagcaaac tcacgaaagc aaaacagagc aagctcaacc
     3661 aaaatttcaa gtatttcaag aatttgatca cagtgctaag ttgcgtgagc tagtcttttt
     3721 tgctcgtatc gctctcttca gtataaccac tgtgctaatc tctttcttat tactagcact
     3781 aagtttaaca cctgtgtgga gtttcttact agcgatgctg gcaagtctag cagttacatc
     3841 tgcagtctct tcattcattt ggtcactcag acaataaaaa tctcataaaa aagaagctac
     3901 ttatagtagc ttctttttta tttttcttta tcttctgtgt tttctttata atcaatgaca
     3961 atatcttcta cttttgtttc aggagcctta tcttcttttt tagcagtatc ctcgtctgag
     4021 aattttgatt tagcttgatt gacaaaatca ttagcaaagt caactacttc tccgcttttt
     4081 tccttaacgg ctgagacgat atcctctgtt gtcaattcac ctgattcaaa tttaccttta
     4141 taatctttaa aagtatcaac agctaaattt gaatattcac ttgctttatc tttagctatt
     4201 tgatgatatt cttctggatt ttctttatat tctccataga atttttctgc atttttcctt
     4261 agctcttttc ctttttttgt tgttaaaaaa taagcagctg ctgcacctga tgcagcactg
     4321 ataactaaag ttttaaataa tttactcatt ttttgtctcc ttttttacga aaaatttttg
     4381 aagccgcttt tccaatagct aaagctgacc ctgccttagt aacacttgag gttgcactac
     4441 ttgccttttg tccaatgtgt cttgcctgta ggtttaaatc agatacagat tctgataagt
     4501 cagcaatagc tacaaaaaga ggatcaatag tagatacttt accattaaca tcatcaacta
     4561 aaacattagc tttggctaaa atttcattag tttggtaaag agtcacgtta acatcgcttg
     4621 ttaatacttt aatagtttgc ttggtttcct caatcgtttc tgatactttt ttcagaacga
     4681 aaagtattcc taaaactaaa acaacaaacg caatggcaat aattaataca gcaatttcta
     4741 tcataatgcc tcctctgttt aataatatga aatatgttgt tcacgacgat gataaataat
     4801 catgacaatt ccgataataa ctaaaataac tgataaccat tgtgacacgc gcaaacccgc
     4861 caaataaagg ctatctgtcc tcatcccttc aataatgaac cgtccacatc cataccaaac
     4921 taaataaaaa catgccactt caccttgttt taaagtgcgc ggtcgatgac gaatagacat
     4981 gatgatgaca aaacctagga agttccaaag agactcatat aaaaatgtag gtactcggta
     5041 agctccatca atatacattt gattttttat aaagtttggt aagtaattca gattttttac
     5101 tgccctaccg tatgcttctt gattaataaa attcccccat ctacctatgg cttgggcaat
     5161 catcactcct ggagcagcaa tatccagaaa atcaatggga ttaatcaagc gacgatatga
     5221 aaaaatcaca aggagaatgg ctccggtaat taaccctccg taaatagcaa taccaccgtt
     5281 ccaaatggcg ataatctcta ctgggtgctt agagtaataa gcccattcga agataacata
     5341 ataaatacga gctccaacaa tggatagtgg aaaagccatg agaatgaaat ctagaatatc
     5401 atccgactta atgttcttac gaggggcctc tttcattgct aagtaaactg ctaacaacat
     5461 accagacact atgcaaatag cataccagcg aatagaaaag gggcctaatt gtattgctac
     5521 tggattaatc atcattgacc tcgttctgac taatcaaatg tgttaaacga tcctcaaaag
     5581 tctgggtagc atcaaacccc atttgtttag ctctgtgatt catagccgct gcttcaataa
     5641 ctactgacac gtttcggcct gttttaacag gaatacgtat ccgaggaacc ttaactccag
     5701 ataattcgat ctcttcattg ccatttccga gtctatcaaa aacttttcca gtttcaaagt
     5761 tttcaaggta tatagctaat tgcacttgcg atgaatcttt aaccgcacta gctccatata
     5821 ggctcataat atcaatgatt cccacacctc taatttcaag taaatgacgt aatatctcag
     5881 caggctctcc ccaaagcgtc tcttcgtctt tagcataaac atcaacacgg tcatctgcta
     5941 ccaaacgatg tcctcgctta actagctcta gtcctgtctc actcttacca ataccggaat
     6001 ctccttgaat cagaacaccc ataccataga tatccatcaa gacaccatga acacttgtcc
     6061 tctcagctaa gcaagaatct aaataccaag aaacttcacc tgataaacga cttgtagggg
     6121 ccttactttg aagaatagca ataccggtat ctttagccgc ctcatacatc tcttctggaa
     6181 tttctaaatc acgtgctaca acaatagctg gtgtttcttt ttggaacatc tcacgtaaca
     6241 cttgataacg attatgtcct gtcatagcca ttaaatatga ccactctttc ataccaacaa
     6301 gttgcaaacg ctctggtgca taataatcaa aataccctgt catttctaga ccaggacgtg
     6361 aaatgtcagc tgttgtaatc cgcttagata atagatgttc atcaccatag atgacattta
     6421 actttaagcg gtcaactaac atttgaacag taactgccat acttactcct tcttataaaa
     6481 gtgaatatcc ctattgctat accctacaat ttaaagttga gcaaaggtac taaacacttt
     6541 cattaattta cacccacatt atatcatact ttttactaaa agtaagcgct aacgcgtttt
     6601 tctgaaaaaa agaagatatt ttgaaaaaat ggtctaaata aagctaagaa aaaaggagat
     6661 tcgtctagca aatctccttt atatgaatta ccagaaccaa ccgtcatcat caacaggttc
     6721 agcatcttta cgtcttctgg gaccttgtct tcttgtttca ataatatctt ctttataggg
     6781 taagaaaaca gctaataaaa tatatagcaa aagaccaaat ttggtaaaat acaaaatcag
     6841 tgcaatcaac acccgtgaaa gagctaaatc ccagttatac ttatcagcta gaccggcgac
     6901 aacaccgcag accaacttac cttttcgttg tttgtagaag cttgatttca tacctatcct
     6961 tctttctatc tactctatat tttaattata actactttga cagtaaaatt aaataggcct
     7021 tacgactgat tttaaaaact ctctatcaag cgaccatgac aataaccaca agcaaacttg
     7081 cgtaaattaa cacgtctcct acgtcggtat acttgaccac aagattggca agagtaatga
     7141 tgataattca ctttaacagc cgtaactcta ggggtatatc gtaagccatc gactttttct
     7201 agtaactctt taaaagcttt atccctatgt cgatatcctt taccttgtat atagagatga
     7261 taatggcaaa gctcatgtct tacaatctta cgaaaaatat caataccaaa ctcacgataa
     7321 attttgggat taaagtcaag gtgtctatcg tttggaaaga agcgtcctcc ggttgttctt
     7381 aagcgattat tccaatgtgc agtatgtaaa aactgacatc caaaatcttc tatagacacc
     7441 ttcttaacat aattagtcaa gtccgtctgg cttgataaga ctaagtccaa ttctgccacg
     7501 ctccgcatct acctctacaa cccaaacagt tacaatatca ccaacagaga gatatttgct
     7561 tggatgtggt aaaggaggca tttttctagt ttttttatcg cgttttcgct taataaggcg
     7621 agactggtgg attagaccat cttcatgaac accaatatca acaaaagcac caaaatcaac
     7681 gacgtttcga acagttcctt gcaattcctg tccaactttt aaatcactga catctaaaac
     7741 atcatgtctc aaaacaggcg cttcaaaatc atcacgcaaa tcacgacctg gtttcaaaag
     7801 atcttctata atgtctttta atgtttcttg acctacccca atactttctg ctgttgcaat
     7861 taagtcaagg tgttgtaact tctcttttgc aagatcatct aattctttga tagtcagttg
     7921 gtcaagcaac tttttaacag cttcataaga ctcagggtga acgcctgtat tatccaagaa
     7981 atttttagca ttaggaatac gtaagaatcc agcagcttgc tcaaaagctt ttgctcctaa
     8041 tctgggtact ttcttaattt cagcacgtga cttaatttgc ccattctctt cacgatattt
     8101 aacaatattt tcagatattg ttttatttaa acctgaaacg tgagctaaca aggctggact
     8161 tgctgtatta acattaacac ctacttggtt aactacagtt tcaacaacaa aatcaagatt
     8221 ttctgctaat tttttttgac taacatcatg ttgatattga cccacaccaa tagattttgg
     8281 atcaattttg actagttctg ctaacgggtc ttgcagacgt cttgcaattg agatagccga
     8341 acgtttttca actgttaagt ctggaaattc atgacgtgcc aattctgagg ctgaataaac
     8401 agaggcacca ctttcgttaa caatcacata ggatacgtct ggaaaatctt gtaaaacttc
     8461 tgcaacaaat gcttctgatt cacgactcgc tgttccattc cctatagcaa taatttctat
     8521 attgaattct ttgataagtt ttgctaactc tatttttgat tgttcgattt ttgcttgatt
     8581 agctggagga actggataaa tcacttgtgt tgtcattaat tttccagttt ggtctactac
     8641 tgctaatttt gctcctgtac gaaaggcagg gtcaaaacct aacaccattt tccccttaag
     8701 cggtgagact aacaataagt tacgaagatt ctctgaaaac agtgaaattg caccatcttc
     8761 tgctccttca cttagttctg tacggatacg gcgttccata gctggaacaa tttttttctt
     8821 gacagtctgt acaataaggt catctatata ttggctagtt tctttaaatc gaacagcaaa
     8881 aaagcgaaac attttttcaa ggttatgctc aaaattaacc ttaagaacac ccattttctc
     8941 accacgattc aatgctaaaa cttgatagcc atggagttta cttatttttt cagaaaagtc
     9001 atagtagatt ttgaagacct gtttttcatc aagtgactca tctttaacaa ctgcagtgat
     9061 tgagctgtag ttccagatct cattatatgt ccatgaacgt aatttattat cctcagaaaa
     9121 agcttcgata agaatatcta ctgctccaga aagggccttc gtagttgtct caaaaccatc
     9181 tgtaagatag ttttgagctt cttcctcaag gttatcctta ttttgtaaaa taagacgtgc
     9241 taaaggaaaa agacctgctt cacgtgcaat cgtagctttc gtacgacgtt tttctttata
     9301 aggtaggtag agttcctcaa catctgctag tttagttgct tcttcaatag cctttttgag
     9361 ttcttgagtc aactttcctt gttcttcaat tttggctaag accgtagttt tacgatctga
     9421 aagagctgtc atactcttat caaggtcaat aattgatttg atttgaactt catccaaatt
     9481 acctgtcatt tctttacgat aacgggcaat aaaaggaata gtatttcctt catctgttaa
     9541 ttctaaaacc ttttcgattt gtgacgcttt aatacctaaa tcaatagcaa tttgtgtaat
     9601 atttaaattt tccataacgc ttttattata gcatattttt gacttacgaa gagttttaaa
     9661 aagcgatttc aagatagcgt ttttataatc taacaataaa aaacagacca ggtctgcttt
     9721 aattcttttc aaaatatttt cgaacaattg gtgctacttc ctcaccataa agtttgatgg
     9781 catttaggac gtctttgtga ggcatagagc caactggtag atgtaacata aaacggtcta
     9841 actcaagcgc ttcaacaagt cctataattt tatgtgcaac cacttcagga cttcctacaa
     9901 aaatagcacc ttctggacct actgaacgta aatactgctc tttagtcatt tcagaccaat
     9961 gagggcgtcc cttagcaata ttatcgacgg tctgtttcgt agggaaaaaa taacggtcaa
    10021 tagcggtttg attatcctct tcaatccatc cccaagagtg agcagcaact tttagttgtt
    10081 ctggtttgtg tccattacga ctaccaactt ctttataaat atggaccaat tgacgaaagg
    10141 ctttgggatt cccaccaata gttgcataaa caattggcaa accttgttct gcaatacgaa
    10201 ttgtagaatc aacatttcct cctgttgcca cccatattga taactgtctt tgtaatgctc
    10261 ttggataaat tggtcgctca ttaactgttt gtgtcaaatg acctttccaa tcgagatttg
    10321 tcgctgagtt aattgctaac aacatatcca ttttttcatt aaataaatca tcataatccg
    10381 ctaaatcgta tccaaacaat ggaaaagact caataaagga accacgccct gccataattt
    10441 ctgctctacc atttgaaagt gcgtcaatcg ttgaaaattg ctgatagacg cgaataggat
    10501 cattggaaga gagaatcgtt actgcactag ataaacggat attattagtt ctaaccgctc
    10561 ctgctgctag gataatttcg ggtgcagaga ccgcaaaatc ttcacgatgg tgctctccaa
    10621 taccatatac atctaaacca acttgatcag ctagttcaat ctcagcaact aattggcgaa
    10681 tcctctcagg atgtgaatag ctttggtttg tttcttcaag gattgttgtc tctccgaatg
    10741 ttgtaatacc taattcaatc ataatcttac ctcatcaatc tactaataac attttatcac
    10801 tatatagtat catttccaat cgatttgttt ttttatgtta aaaataagct aatatagtat
    10861 aataggttat tatataaaga aagaggtgcc attaatggct attacgcaca aacgtcaaga
    10921 tgacttagaa agtatgttcg ctagttttgc taaagtacca aaacctaaaa aagttgattc
    10981 tgattcaaaa cctgaacaga aagatgattc tcagaaagat taagttctat ggatatcttt
    11041 aatcaattac cggatagtgt ccttcaatgg tttgccattt tcatttccat tattattgag
    11101 gcacttcctt ttgttttgct tggaactatt ttatcaggaa ttattgaagt ctttataaca
    11161 cctgatatag tcaataagtt cttaccgaaa aataaatttc tcagagtctt atttggaacc
    11221 ttcgttggct ttgtctttcc ttcttgcgaa tgtggtatca ttcctattat taatcgattc
    11281 cttgaaaaga aagtgccttc atacacggca gttccgtttc ttgctactgc tcctattatt
    11341 aatcctattg ttttatttgc gacctactca gcatttggaa attctatacg cttcctgata
    11401 ttacgctttg taggtgctac cattgtagcc attgctttag gagtcatgct tgccttttta
    11461 gtagatgata atattctaaa agaagacgct aaacctactc attttcatga ttattctgat
    11521 aagaagtggt atcaaaaaat ctttctagcc ttagctcatg ccatcgatga attttttgac
    11581 acaggacgct atctagtatt tggcacttta attgcttcag ctatgcaaat ttatttacca
    11641 actcgagttc ttactactat cggacacagc cctatcacag ccatcttagt tatgatgttg
    11701 cttgctttca tcttgtcatt gtgtagtgaa gctgacgctt ttataggtgc ttctttatta
    11761 tcaacctttg gaatcgcgcc agtgatggct ttccttttaa ttggacccat gattgatatt
    11821 aagaatttaa tgatgatggt aaattctttt aaaacaaggt ttattgtcca attcattagt
    11881 gtatcttctc ttattattat tatctattgt ttatttgtgg gggtaatata atgattcgat
    11941 ttcttattct tgctggttat tttgaattaa gcatgtattt aaagttatca ggtaaactaa
    12001 atcagtatat taatactcat tatacctatt tggcctatat ttcaatggtc ctatctttta
    12061 ttttagcaat tgtacaacta attatttggg ttaaaaatat gaaaatgcat agtcacctac
    12121 atggtaaaat agctaaatca acaagcccaa tgattttagt atttcccgtt ttagtaggtt
    12181 tgttagtacc tacagttagt ttagactcga ccacagtctc tgccaaggga tataatttcc
    12241 ctctagcagc cggctctact ggtactgtca gtcaagatgg gacacgcgtt caatatttga
    12301 aaccagatac cagcacttat tttacttctt cagcctatga aaaagaaatg caaaaagagc
    12361 ttaaaaaata taaaggctct ggtacattaa ccatcacaac tgaaaactac atggaagtta
    12421 tggaactaat ttatctctat cccgagcaat tcatggatcg ccaaatacaa tatactgggt
    12481 ttgtttataa cgaacccaaa cacgaaggat atcaatttat tttccgcttt ggaatcattc
    12541 attgtattgc tgattcagga gtttatggtc tactaacaac tggaaatcaa aaaagttatc
    12601 cggacaatac ttgggtaact gttagaggta caattaaatc cgagtataat caattgttac
    12661 aacaaaactt accagtctta cacattgaag agtctcgtca agtttctaaa cctaataatc
    12721 cttatgttta tcgtgtcttt t
//
LOCUS       DATRUK010000060        11947 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.060, whole genome shotgun sequence.
ACCESSION   DATRUK010000060 DATRUK010000000
VERSION     DATRUK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11947
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.060"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..333
                     /locus_tag="VBE34_001605"
     CDS             <1..333
                     /locus_tag="VBE34_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7264309.1"
                     /translation="IVLNAVEAIDKQKKGTISVHTEELEDFIKIEISDNGPGIPDKMK
                     HMIFKPGFSTKFDANGDIYRGIGLSHVRILMEEQYQGTITVCPNQPNGTTFTLLFRKN
                     RFKLGDNT"
     gene            330..1163
                     /locus_tag="VBE34_001606"
     CDS             330..1163
                     /locus_tag="VBE34_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEO7264310.1"
                     /translation="MKFYIIDDDPTITMILQDIIEEDFNNTVVRVNNVSSKAYNELLI
                     ADVDIVLIDLLMPILDGVTLVQKIYKQRSDLKFIMISQVKDNDLRQEAYKAGIEFFIN
                     KPINIIEVKSVVKRVTDTIEMQKKLNTIQNLLENTPSYQKPITTSNLTKIRSILSYLG
                     ITSETAYTDILNICELLLKQELNFAQFDFQKELSIDEHQQKIILQRIRRAVKKAMINM
                     AHLYIDDFENELTLQYANALFGFQNIHNEAQLIQGKSMYGGKISLKRFFDELILQSKT
                     F"
     gene            1385..2107
                     /locus_tag="VBE34_001607"
     CDS             1385..2107
                     /locus_tag="VBE34_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7264311.1"
                     /translation="MISFENVSKSYGDHTIIDNISCHIQRGEFFVLVGASGSGKTTIL
                     KMINRLIEPSQGAITLDGENITSLDLRQLRLETGYVLQQIALFPNLTVGENIELIPEM
                     KGWSKGDQKKAASDLLDKVGLPAKDYFNRYPHELSGGEQQRIGILRAIVAKPKVLLMD
                     EPFSALDPISRRQLQDITKQLQSELGITLVFVTHDMKEAMRLADRICVIKEGKIVQLD
                     RPEIIQNNPSDQFVRTLFEEEN"
     gene            2110..3623
                     /locus_tag="VBE34_001608"
                     /pseudo
     CDS             2110..3623
                     /locus_tag="VBE34_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019319700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
     gene            complement(3702..6242)
                     /gene="mprF"
                     /locus_tag="VBE34_001609"
     CDS             complement(3702..6242)
                     /gene="mprF"
                     /locus_tag="VBE34_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEO7264312.1"
                     /translation="MKKLIEKVKSLTSVIKIVFFISVLVLIIVEMIHLKRTISVEQLK
                     SVFGQLSPMNLFLIILVGVIAVLPTTGYDFVLNGLLRTDKSKRYILQTSWCINTFNNL
                     SGFGGLIDIGLRMAFYGKKGQEKSDLREVTRFLPYLISGLSFISVIALIMSHIFHAKA
                     SVDYYYLVLIGASMYFPVIYWISGHKGSHYFGDMPSSTRIKLGIVSFFEWGCAAAAFI
                     IIGYLMGIHLPVYKILPLFCIGCAVGIVSLIPGGLGSFELVLFTGFAAEGLPKETVVA
                     WLLLYRLAYYIIPFFAGIYFFIHYLGSQINQRYENVPKELVSTVLQTMVSHLMRILGA
                     FLIFSTAFFENITYIMWLQKLGLDPLQEQMLWQFPGLLLGVCFILLARTIDQKVKNAF
                     PIAIIWITLTLFYLNLGHISWRLSFWFILLLLGLLVIKPTLYKKQFIYSWEERIKDGI
                     IIVSLMGVLFYIAGLLFPIRAHITGGSIERLHYIIAWEPIALATLILTLVYLCLVKIL
                     QGKSCQIGDVFNVDRYKKLLQAYGGSSDSGLAFLNDKRLYWYQKNGEDCVAFQFVIVN
                     NKCLIMGEPAGDDTYIREAIESFIDDADKLDYDLVFYSIGQKLTLLLHEYGFDFMKVG
                     EDALVNLETFTLKGNKYKPFRNALNRVEKDGFYFEVVQSPHSQELLNSLEEISNTWLE
                     GRPEKGFSLGYFNKDYFQQAPIALVKNAEHEVVAFANIMPNYEKSIISIDLMRHDKQK
                     IPNGVMDFLFLSLFSYYQEKGYHYFDLGMAPLSGVGRVETSFAKERMAYLVYHFGSHF
                     YSFNGLHKYKKKFTPLWSERYISCSRSSWLICAICALLMEDSKIKIVK"
     gene            complement(6226..6969)
                     /locus_tag="VBE34_001610"
     CDS             complement(6226..6969)
                     /locus_tag="VBE34_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7264313.1"
                     /translation="MTENWLHTKDGSDIYYRVVGQGQPIVFLHGNSLSSRYFDKQIAY
                     FSKYYQVIVMDSRGHGKSHAKLNTISFRQIAVDLKDILVHLEIDKVILVGHSDGANLA
                     LVFQTMFPDMVRGLLLNSGNLTIHGQRWWDILLVRIAYKFLHYLGKLFPYMRQKAQVI
                     SLMLEDLKISPADLQHVSTPVMVLVGNKDIIKLNHSKKLASYFPRGEFYSLVGFGHHI
                     IKQDSHVFNIIAKKFINDTLKGEIVEKAN"
     gene            complement(7103..7459)
                     /locus_tag="VBE34_001611"
     CDS             complement(7103..7459)
                     /locus_tag="VBE34_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEO7264314.1"
                     /translation="MSTLTIIIATLTALEHFYIMYLETLATQSNMTGKIFSMSKEELS
                     YLPVIKLFKNQGVYNGLIGLFLLYGLYISQNQEIVAIFLINVLLVAVYGALTVDKKIL
                     LKQGGLPILALLTFLF"
     gene            complement(7526..9844)
                     /locus_tag="VBE34_001612"
     CDS             complement(7526..9844)
                     /locus_tag="VBE34_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEO7264315.1"
                     /translation="MLDELKAIIKSPKLWITMAGVALIPTLYNVIFLSSMWDPYGNTK
                     NLPVAVVNQDKSAKLNGKTISIGKDMEDNLSKNDSLDFHFTTAKRAEKELEKGHYYMV
                     ITFPKDLSRKATTLMTEKPERLNITYKTTKGRSFVASKMSETAANKLKDEVAESITGT
                     YTESVFKNMGSMKTGINKAADGSQELLNGSNKLQNGSQTLTSNLDVLASSSQTFSGGA
                     NKLNSGINLYTDGVGTLSNGLETLSDGVTAYTTGVHKLSEGSQKLDDKSQALVEGSEK
                     LTVGLQQLSQATQLKPEQERTLQNLSDGLKNLNQIITNLQSTATTDSDTNSKLFNFLS
                     TIESSTTALMNTAAADKQKQMTAVQSTSAFKSLTPEQQSQITSAVTGTPTSAETIAAN
                     ISSNIENMKTVLSEASSSAPSNNNSQNFQTLSGTANNLVLKAISDLDKIQKLPTATKQ
                     LYQGSQTLTKGITDYTNAVGQLRKGAETLDSKSNQLISGTQKASQGAQTLDSKSDQLR
                     DGAGQLASGSDRIADGSNKLAGGGHQLTDGLTELSGGVSQLSSSLGKAGDQLSMVSVN
                     KDNANAVSSPVTIKHEDYDSVDTNGVGMAPYMISVALMVVALSANVIFAKALSGKEPA
                     NRFSWAKNKLLINGFIATLAATILFFAVQFIGLKPDYPGKTYFIILLTAWTLMALVTA
                     LVGWDNRYGSFLSLLILLFQLGSSAGTYPIELSPKFFQTIQPFLPMTYSVSGLRETIS
                     LTGDVNHQWRMLVIFLVSSMILALLIYRKQED"
     gene            9983..10522
                     /locus_tag="VBE34_001613"
     CDS             9983..10522
                     /locus_tag="VBE34_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEO7264316.1"
                     /translation="MVNDTRREKTKRAIEAAMITLLKDQSFDEISTINLTKTAGISRS
                     SFYTHYKDKYEMIDQYQQSLFNKVEYIFDRNQFKKEDALLEIFQFLDRESLFAALLTQ
                     NGTKEIQTYILNKLQLMLSKELPVVNPDATKSDINRLYYSVYLSHAIFGVYQMWITRG
                     KKESPQQITQVLLSLLPQT"
     gene            complement(10608..10904)
                     /locus_tag="VBE34_001614"
     CDS             complement(10608..10904)
                     /locus_tag="VBE34_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEO7264317.1"
                     /translation="MNLNDRLKIEEMEEKYDSFKPRINALVEAIDDFQKHYEDYVKLR
                     EFYGSEDWFRLSEQTENNLKCGVLSEDQLFDFIGEHNELVGQFLDMSSQMYRHL"
     gene            complement(11148..11759)
                     /gene="rpsD"
                     /locus_tag="VBE34_001615"
     CDS             complement(11148..11759)
                     /gene="rpsD"
                     /locus_tag="VBE34_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEO7264318.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKAKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
BASE COUNT         4109 a         2218 c         1851 g         3769 t
ORIGIN      
        1 atcgttttaa atgcagtaga agctattgat aaacaaaaaa agggtacaat cagtgttcac
       61 acagaagaat tagaagattt tattaaaatt gagattagtg ataacggacc aggaatacct
      121 gataaaatga aacacatgat tttcaaacct ggattttcga ctaaatttga tgctaatggt
      181 gatatctatc gaggaatcgg gctatcccac gtgcgtattt taatggaaga gcaatatcaa
      241 ggaacgataa ctgtttgtcc taatcagcca aatggaacaa catttacact gttattccgt
      301 aaaaatcgat ttaagttagg agataatacg tgaaattcta tattattgat gatgacccca
      361 ctatcactat gattttacaa gatattattg aagaagattt caataataca gtagtcagag
      421 tgaataatgt ctccagtaaa gcatacaacg aattgctgat tgcagatgtt gatatcgtat
      481 tgattgattt attaatgcca atattagacg gtgtaacatt agtccaaaaa atctataaac
      541 agcgttccga tttaaaattt attatgattt cccaagtcaa agataatgat ttacgacaag
      601 aagcatataa agcaggaatc gaatttttta ttaataaacc cattaatatt attgaagtaa
      661 aaagcgtcgt gaaacgtgtc actgatacta tcgaaatgca aaaaaaatta aatacaatcc
      721 aaaatctcct agaaaatacg ccatcatatc aaaagcctat aacgactagt aacctcacta
      781 aaatccgttc aatactcagt tatttaggta ttacttcaga gacagcctac acagatattc
      841 ttaacatctg tgaactttta ttaaaacaag aattaaattt tgcccaattt gattttcaaa
      901 aagaactttc tatcgatgaa caccaacaaa aaatcatttt acaacgtatt cgtcgtgccg
      961 ttaaaaaagc tatgataaat atggctcatc tgtacattga tgattttgaa aatgaattaa
     1021 cactgcaata tgctaatgca ttgtttggat ttcagaatat ccataatgag gcacaactta
     1081 ttcaaggaaa aagcatgtat ggtggtaaaa tttcacttaa acgttttttc gatgaactta
     1141 ttttacagag taaaacgttt taagacttgt ttaagcatgg ccttatatac tataaccatc
     1201 ctaaaacttt tcttttcata aatctccaac aaacaccttg tctaccgcaa ggtgtttgtt
     1261 tttgcttcta gtttatcgtt tctgacaatt taaatgtcag atagttgttt gaatgtctac
     1321 tttatgctaa tataacccta atatcatacg acctacactc gttacgcact agaaagtaaa
     1381 ttacatgatt tcatttgaaa atgttagtaa atcatatggt gatcatacca tcattgataa
     1441 tattagttgc catattcaaa gaggagaatt ctttgtcctt gtgggggcca gtggcagtgg
     1501 taaaactacc atcctcaaga tgattaatcg tctcatcgaa ccaagccaag gcgctattac
     1561 tttagatggg gaaaacatta catcacttga tttgcgacaa ttacgcttag aaactggtta
     1621 tgtcttgcaa caaattgcac ttttccctaa tttaacagtt ggagaaaata tagaacttat
     1681 ccctgaaatg aagggctggt caaaaggtga ccaaaaaaaa gccgctagcg accttcttga
     1741 taaggtgggt ttacctgcta aggactactt taatcgctat cctcatgagc tctctggtgg
     1801 ggaacaacaa cgcataggta tcctacgagc aatcgttgct aaacctaagg tactacttat
     1861 ggatgagcca ttttctgctt tagaccctat ctcaagacgt caattacaag atatcaccaa
     1921 acaattacag tctgaattgg gtattacatt agtgtttgtc acccatgaca tgaaggaagc
     1981 tatgcgtctt gcagatagaa tttgtgttat taaggaaggt aagatagtgc aattggatag
     2041 accagaaata attcaaaata atccctctga ccaatttgtt agaaccctat ttgaagagga
     2101 gaactgagta tgacaacact aatcactacc tttcaagagc gttttggaga ttggactcaa
     2161 agtcttattg aacacctcca gctatcacta ctaacactta ttttagcaac tttaattgct
     2221 attccactcg gtatcatcat tagtcattat aaaaaatcag tcatgttgtc cttcagataa
     2281 ctgggatatt tcaaacaatt ccttctttag ccttgcttgg tttatttata ccttttatgg
     2341 gaattggaac tgtaccagct gtcgttgcac taattattta cgctctattt ccaattttac
     2401 aaaatactgt aacagcactg atgcaaattg atgctaatct cattgaggca gcaaccgctt
     2461 ttggaatgac acgttgggaa aggcttaaaa agtttgagtt agctttaagt atgcctgtta
     2521 tcatctcagg cattagaact gcctctgtta tgattattgg tacagctaca ttagctagtc
     2581 ttatcggagc tggtggcctt ggatcattta ttttgttagg gattgatcgc aacaatcctt
     2641 ctctaattct cataggggca atctcttctg ctgtcttagc tattatattt agtggtttaa
     2701 ttggattact agaaaaggct agattacgaa ccatagccgt ttctggtata cttttactag
     2761 caggcttagg actatcctat gctccaaaat ggatgcctgg aacaaatact gcaacgataa
     2821 ctgttgcagg gaagttagga actgaaccag atattttaat taatatgtac aaagaactta
     2881 ttgaggatca gacagatatc aaagttaagc ttaaacctaa tttcggaaaa acaactttct
     2941 tatatcaagc cctaaaatct ggtgatattg atctttaccc agagtttaca ggtactatta
     3001 catcaagttt acttaaaaat cctcctaaag taagtaataa ccctaaacag gtttacaact
     3061 tagccaaaaa cggtatttta aaacaagata agttgtcact tttgtcacct atggcttatc
     3121 aaaacaccta cgctgtagct gttaaaaaag attacgctga agctaatcaa ctcaaaaata
     3181 tcagcgacct aaaaaaatta gataaactaa aagctggttt cactttagaa tttaaggata
     3241 gagaggatgg gagtattggt ttacagaaac attatggttt aaaccttgat atctcaacct
     3301 tagaacctgc tttacgatac caagctatca atagtaaaga tgtcaatatt attgacgctt
     3361 actctaccga tagtgaatta atccagtatc aacttcaaat tctcaaagac gataagcatc
     3421 tatttccgcc atatcaagga gcaccgctac taaggcaaga tacgattaaa aaataccctc
     3481 aagtaaaaaa agctctcaat aaacttgctg gtcatatcac tgaaaaagaa atgcaagaaa
     3541 tgaactacca agtagctgta aagcataaat cagcagctac agtagctaaa caatatttaa
     3601 aagcacatca tattataaag tagattcaag caatcaatgg tattaaatga tttaaaaaga
     3661 gcatgtcgca tgacatgctc tttttaattg ccaaataaag cttatttaac aatcttaatt
     3721 ttactatctt ccattaatag ggcacaaata gcacaaatta accaggacga acgagaacaa
     3781 gaaatataac gttccgacca caatggtgta aacttcttct tatacttgtg taaaccatta
     3841 aatgagtaga aatgactacc gaaatgatag acaagatacg ccattctctc tttagcaaaa
     3901 cttgtttcaa cgcgaccaac tcctgataaa ggtgccatcc ccaaatcaaa atagtggtat
     3961 cccttctctt gataataaga gaataatgat aaaaagagga aatccataac gccattcgga
     4021 attttctgtt tatcgtgacg cattaaatca atagagataa tactcttttc atagtttggc
     4081 ataatattag caaaagcaac aacttcgtgt tcagcatttt ttaccaaagc tattggggct
     4141 tgttggaaat aatctttatt aaaatatcct agtgagaaac ctttttcagg acgtccttct
     4201 aaccaagtat tagaaatctc ttccaaacta tttagtagct cttgactatg tggcgattgt
     4261 acaacttcga aatagaaacc atccttttca actctattta gggcatttct gaaaggtttg
     4321 tacttattcc ctttaagagt aaacgtttct aaattaacta aagcatcctc accaactttc
     4381 ataaagtcaa aaccatactc atgtaaaagt agtgtcaact tctgtccaat actgtaaaaa
     4441 acaaggtcat agtctagctt atcagcatca tcaataaacg attcaatagc ttcacgaata
     4501 taagtgtcat caccggctgg ttcccccata ataagacatt tattattgac aattacaaat
     4561 tggaacgcaa cgcaatcttc tccatttttt tggtaccagt agagcctttt atcatttaaa
     4621 aaggctaaac cgctatccga agaaccaccg taagcttgaa gtagtttttt ataacgatcc
     4681 acattgaaca catcaccaat ctgacaagat tttccttgta aaatcttaac caaacataaa
     4741 taaacgagag taagaatcaa cgtagccaat gctatcggct cccatgctat gatataatgc
     4801 aggcgttcaa tactaccacc tgtaatatga gccctgatag ggaatagtag tcctgcaata
     4861 taaaatagaa ctcccattaa actaacgata atgattccat ccttaatacg ctcttcccag
     4921 ctataaataa attgtttttt atagagagtt ggcttaatga ctaataagcc taacaatagt
     4981 aaaataaacc agaaagatag tcgccaacta atatgaccta aattaagata aaacaatgtc
     5041 aaagtaatcc agataatagc aattggaaaa gcatttttca ctttttgatc aatagttcta
     5101 gctaagagaa taaaacaaac ccccagcaat aaacctggaa actgccataa catttgttct
     5161 tgtaatgggt ccaagcctag cttctgcaac cacataatat aagtaatatt ttcaaaaaat
     5221 gctgttgaaa atattaagaa tgcacctaaa atacgcatca aatggctcac catggtttgt
     5281 agaacagttg atactaactc tttcgggaca ttttcataac gttgatttat ttgactacct
     5341 aaataatgga taaagaaata gatacctgca aagaatggaa taatatagta ggctaaacga
     5401 taaagtaata accatgcaac cacagtttct ttaggtagtc cctcggcagc aaaccctgta
     5461 aatagaacta attcaaaact tcctaatcca ccgggaataa gggatacaat cccgacggca
     5521 caaccaatac aaaatagtgg taaaatttta taaactggta gatgaatgcc cattaaataa
     5581 ccgataatta taaacgctgc ggctgcacat ccccattcaa aaaaagaaac aatacctaat
     5641 tttatacgag tactagatgg catatctccg aaataatggc ttcctttatg accagaaatc
     5701 caataaataa caggaaaata catactagca ccaattaata ccaaataata gtaatcaaca
     5761 ctagctttgg catgaaaaat atggctcatg attaaggcaa tcacactaat aaatgacaga
     5821 ccagaaataa gatagggtaa aaaacgagtc acttctctta ggtcactctt ctcttgacct
     5881 tttttaccat aaaaagccat gcgcaaccca atatcgatta agccaccgaa tcctgacaag
     5941 ttattaaaag tgttgataca ccaactagtc tgtaaaatat accttttgct tttatctgta
     6001 cgtaaaagtc cattcagtac aaagtcatat ccggttgtcg gtaagacagc gataaccccc
     6061 acaaggataa ttaagaaaag attcattgga gataattgcc caaaaacact ctttagttgc
     6121 tcaacagaaa tagttcgttt caaatgaatc atttcaacaa taataagcac taaaacagat
     6181 ataaaaaata caattttaat cacagaagtc agtgatttga ctttttcaat tagctttttc
     6241 aacaatttct cctttcaacg tatcgttgat aaactttttt gcaataatat taaaaacatg
     6301 ggaatcttgc ttaataatgt gatgcccaaa gccaactaaa gaataaaact ccccccttgg
     6361 aaaataagaa gcaagtttct tagaatgatt taacttaatt atgtccttat ttccaaccaa
     6421 aaccattaca ggagttgaca catgctgtaa atcagctgga ctaatcttca aatcctccaa
     6481 cataagcgaa ataacttgag ctttttgcct catatacgga aagagtttcc ctaaatagtg
     6541 aaggaattta taggcaatcc ttactaaaag aatatcccac catcgctgac catgaatagt
     6601 caggttccct gaattaagca aaagccctct aaccatatct ggaaacatcg tttgaaaaac
     6661 taaagctaaa ttagcaccat cgctatggcc taccaatata actttatcaa tctctaaatg
     6721 aactaagata tcctttaagt caactgctat ttgcctgaaa ctaatggtat ttagctttgc
     6781 atgacttttg ccatgccctc tactatccat aacaataact tggtaatact tagaaaaata
     6841 tgctatttgc ttatcaaaat agcgactact taagctattg ccatgtaaaa aaacaatcgg
     6901 ttgaccttga ccaacgacac gataataaat atctgaacca tctttagtat gtaaccagtt
     6961 ctcagtcata accaaagtca cctctctttg aattcatagt tatattatac tatttttaaa
     7021 gacaaattac ttaaactacc tgtattatga gaaaaaaatg attactaaaa gccgttcaac
     7081 taaatcgaac ggctaagtag tattaaaata agaatgttaa aagagctaat ataggtaaac
     7141 caccctgttt taataagatt tttttatcaa ctgtcaaagc accataaaca gcaactagca
     7201 acacattgat taaaaaaata gctacaattt cttgattctg tgaaatatat aacccataaa
     7261 ggaggaatag accaatcaag ccgttgtata caccttgatt cttaaaaagt ttaataacgg
     7321 gtaaatatga caactcttct ttagacatac taaaaatctt cccagtcata tttgactggg
     7381 tggctaacgt ctccaaatac ataatataaa aatgttccaa agcagttaat gttgcaataa
     7441 ttattgttaa agtagacata tcttctcctc ctaaatactt tgacatagca tatcatactg
     7501 ttagtctatc actagatact ttctattaat cttcttgttt acgataaata agaagagcaa
     7561 gtatcatcga tgatactaaa aagattacta gcattctcca ttgatggtta acgtctcctg
     7621 tcaacgagat ggtctccctt aatcctgaaa cagagtaagt catcggtaaa aatggttgaa
     7681 ttgtttgaaa gaacttagga ctcaattcta ttgggtaagt tcctgcgctt gaaccaagct
     7741 ggaataataa tattaataac gacaagaagg aaccatacct attatcccat cccactaaag
     7801 cagttactaa tgccatcaaa gtccatgctg tcaataggat aataaagtag gtttttccag
     7861 ggtaatctgg tttaagacct atgaattgaa ctgcaaaaaa taagatagtt gctgctagag
     7921 ttgcaatgaa tccattgatt aataatttat ttttagccca actaaaacga ttagctggct
     7981 ctttgccaga taaagctttc gcaaaaataa cattggctga taaagctacc accatcaacg
     8041 caactgaaat catgtatggt gccattccaa caccattcgt atcaacgcta tcataatctt
     8101 catgcttgat agtgactggg ctagatacag cattggcatt atccttgttt acagaaacca
     8161 tggacaactg gtccccagct ttacccaaag aacttgataa ttgagaaaca ccacctgaca
     8221 actctgtcaa cccatctgtc aactgatgtc ctcccccagc aagcttatta gaaccatctg
     8281 caattctatc agaaccacta gctaattgcc ccgctccatc tctcaattga tccgatttac
     8341 tatctaaagt ttgagctccc tgacttgctt tttgtgttcc tgaaatcaat tgattggatt
     8401 tactatctaa agtttctgca cctttgcgta attgccctac agcattcgta taatccgtga
     8461 ttccttttgt caaggtttga ctaccttgat ataactgctt agtagccgtt ggcaattttt
     8521 gaattttatc taaatctgag atagctttta atactaaatt gttagctgtt cctgacaaag
     8581 tttgaaaatt ttgtgagtta ttattagaag gggctgaact agatgcttca ctaagcacag
     8641 ttttcatatt ttctatattt gaagaaatat ttgctgcaat tgtctcagct gaagtaggtg
     8701 ttccagtaac cgctgaggta atttgagatt gttgttcagg ggttaaactt ttaaaagctg
     8761 acgttgattg taccgcagtc atttgctttt gtttatcagc agcagccgta ttcatcaaag
     8821 ccgttgtact tgattcaata gttgataaaa aattaaataa cttactatta gtatcgctat
     8881 cagtagttgc tgtcgattgt aaatttgtaa taatctgatt tagattcttt aatccatctg
     8941 aaaggttctg caaagtacgt tcttgctctg gttttagttg agttgcttgt gacaattgtt
     9001 gtaaaccaac cgtcaacttt tctgatcctt ccaccaatgc ttgagactta tcatctaatt
     9061 tttgactacc ttcactcaac ttatgcacac ctgttgtgta agcagtaaca ccatctgata
     9121 atgtttctaa cccgttagat agggttccca caccatctgt ataaaggttt attccactat
     9181 ttagtttatt agctcctcca gaaaaagttt gactagagct tgctaaaaca tctaaattac
     9241 ttgtcaaggt ttggctccca ttctgtagct tattagaacc atttaataat tcttgactcc
     9301 catcagcagc tttattgata cctgttttca tagaacccat atttttaaaa actgattcag
     9361 tataagtccc tgtaatagat tctgctacct catcttttag tttattagct gctgtctcac
     9421 tcatttttga agctacaaaa ctacgcccct tagtagtttt ataagtaata tttaaccttt
     9481 ctggtttttc tgtcatgaga gttgtcgctt ttctagaaag atctttagga aaggtaataa
     9541 ccatataata gtgacccttt tctaattctt tttctgctct tttagctgtt gtaaaatgga
     9601 aatctaagct atcattttta gacaaattgt cctccatatc tttgccaatt gaaatagttt
     9661 ttccatttaa tttagccgat ttatcttggt taacgactgc cacaggtaaa tttttagtat
     9721 tcccataagg atcccacatt gagcttagaa agataacatt atatagcgtt ggaatcagtg
     9781 caaccccagc catcgtaatc cataattttg ggctttttat gattgctttt aattcgtcta
     9841 acattttttc tcctattttg gacatattgt tcattttttt gataaaatat attatacagt
     9901 gatgatagat aatttcaaga taaaaatctc gtttttggac actgtgttta aaaatgtaca
     9961 aaaatattaa ggggtaatta atatggtaaa cgatacgagg agagaaaaaa caaaaagagc
    10021 tattgaagca gctatgatta cgttattaaa agatcagagc ttcgatgaaa tttcaacgat
    10081 taatctaact aaaacagctg gaatcagtcg ttctagtttt tatactcatt ataaagataa
    10141 atatgaaatg attgatcaat accaacagtc actcttcaat aaagtcgaat atatttttga
    10201 tcgcaatcaa tttaaaaaag aagatgcctt gctcgaaatt tttcaattct tagatagaga
    10261 atcattattt gctgctctct taacccaaaa tggaacaaaa gaaattcaga cctacatcct
    10321 taataaatta caattaatgc tttctaaaga attacctgtg gtcaatccag atgcaacaaa
    10381 gtctgatatc aaccgtttgt attatagtgt ctatctcagt catgctattt ttggagtcta
    10441 tcaaatgtgg ataacacgag gaaaaaaaga aagccctcaa caaattacac aagtgctact
    10501 cagtttatta cctcaaactt aaaaccagcc ttccttatag gctggtcatt tttagttagg
    10561 tttattttat cctcattata ggataacaac actaataagg actgacatta taaatgacga
    10621 tacatttgag aagacatatc taaaaattgg cctacaagct cgttatgttc tcctataaaa
    10681 tcaaagagct gatcctcact cagtacaccg catttgagat tattttctgt ttgttctgat
    10741 agtctaaacc agtcttcgct accataaaat tctcttaatt tcacgtaatc ttcatagtgc
    10801 ttttgaaaat catctatagc ttctactaat gcattgattc taggtttgaa gctatcatac
    10861 ttctcttcca tttcttctat ttttaatcta tcatttagat tcataataaa atctccttag
    10921 ttatataaga ttcgacgaga taagttatca ttctcaagta tttgtaacgt taactctact
    10981 atatcctttt tacaaagtaa aaaattactc attacaaact gacagtgttg atttttattc
    11041 taatgttagt ttaaaatctt tttggttcat taaatataat gactcactaa aaagtatata
    11101 gcaaaaaaag cctataaaat aggcttttaa agtatgtaat gtaataatta aagcattttg
    11161 ttgtagaatt caacgacaag tgcttcattg atttctgggt taatttcatc gcgttctgga
    11221 aggcgagtca atgaaccttc aagtttttca gcatcaaatg atacgaaagc tggacgtcct
    11281 aaagtagctt caacagcttc aaggattgca ggtactttca ttgatttttc acgaactgaa
    11341 ataacttgtc ctggagtaac gcggtatgat gggatatcaa cacgtttacc atcaacaagg
    11401 atatgaccat ggttaacaaa ttgacgagct tggcgacgag ttgttgcaag tcctaaacga
    11461 taaacaacgt tatcaagacg acgctctaaa agaaccataa agttgaaacc aagagttcct
    11521 tcttttgctt tagtagcttg tacgaacaag ttacggaatt gtttttcacc aagtccgtat
    11581 gaaaaacgaa gtttttgttt ctcagctaac tgcaagccat attctgaaag cttgctacgg
    11641 ttattaggac cgtgttgacc aggtacgtag ttacgacgtg ccaattcttt acctgtgcct
    11701 gtaagtgata aaccaaggcg acgtgattgt ttccatgatg gaccagtata acgtgacatc
    11761 taaatgtcct cctctaaaat aattgaggaa ataacgattt aaaaaatcct gattcgtgca
    11821 ggggattttc gcctaaacag caaagggtac tagattagtt aaccacctgt tgacgagctt
    11881 catatttttc tgctgttatt tcacacaagc tatattatat catgaaaata atattgaatc
    11941 aagtttt
//
LOCUS       DATRUK010000061        11307 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.061, whole genome shotgun sequence.
ACCESSION   DATRUK010000061 DATRUK010000000
VERSION     DATRUK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11307
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.061"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..494)
                     /locus_tag="VBE34_001616"
     CDS             complement(<1..494)
                     /locus_tag="VBE34_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO7264319.1"
                     /translation="MFTKFFEGLLTYHFLQNAFITAIVIGIVAGAVGCFIILRSMSLM
                     GDAISHAVLPGVAISFILGINFFIGAIVFGLLSSIIITYIKENSVIKGDTAIGITFSS
                     FLALGIILIGLANSTTDLFHILFGNILAVQDSDKYMTIIVGLIVLTLITIFFKELLLT
                     SFDPV"
     gene            complement(496..1212)
                     /locus_tag="VBE34_001617"
     CDS             complement(496..1212)
                     /locus_tag="VBE34_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO7264320.1"
                     /translation="MIISKHLSVSYDNNLVLEDINLRLEGSGIIGILGPNGAGKSTLM
                     KALLGLVDSTGESGIGGDLLPLMGRVAYVEQKTNIDYQFPITVGECVSLGLYKERGLF
                     KRLSKTDWEKVSRVIDQVGLRGFENRPINALSGGQFQRMLMARCLVQEADYIFLDEPF
                     VGIDSISEQIIVNLLKKLSKAGKLILVVHHDLSKVDHYFDQVIILNRHLIACGPIDQA
                     FTRENLSAAYGDAILLGQGD"
     gene            complement(1383..2309)
                     /locus_tag="VBE34_001618"
     CDS             complement(1383..2309)
                     /locus_tag="VBE34_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEO7264321.1"
                     /translation="MKKWLVIVSCFVAFLGLGACANKQDQAKNEKLKVVVTNSILADI
                     TKNIAGNKIDLHSIVPVGKDPHEYEPLPADVEKTTQADLVFYNGINLETGGNAWFTKL
                     IKNAKKKENKDYFAVSDGVDVIYLNGQSGKGKEDPHAWLNLENGIIYSKNIAKQLIAK
                     DPKNKATYEKNRDAYVAKLEKLDKEAKSKFNAIPANKKLIVTSEGCFKYFSKAYGVPS
                     AYIWEINTEEEGTPDQITSLVKKLKQVRPSALFVESSVDKRPMKSVSRESGIPIYAEI
                     FTDSIAKKGQKGDSYYAMMKWNLDKIAEGLAK"
     gene            2476..3123
                     /locus_tag="VBE34_001619"
     CDS             2476..3123
                     /locus_tag="VBE34_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEO7264322.1"
                     /translation="MTPNKEDYLKCIHELGEKNNKISNKKIAEMMQVSAPAVSEMIKK
                     MISEQLIVKDKDLGYYLTKQGLLVVSDLYRKHRLVEVFLVNHLHYTADDIHEEAEVLE
                     HTVSTTFVDQLEKLLDFPQFCPHGGTIPKKGEFLVEINQMTLDQISQLGTYVISRVHD
                     DFQLLKYLEQHRLHINDTIELTQIDPYAKTYHITYNDENLTIPERIASQIYVSAT"
     gene            complement(3163..3852)
                     /locus_tag="VBE34_001620"
     CDS             complement(3163..3852)
                     /locus_tag="VBE34_001620"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEO7264323.1"
                     /translation="MKIGIIAAMEEELKLLVENLEDKSQETVLSNVYYSGRYGEHELV
                     LVQSGVGKVMSAMSVAILVESFKVDAIINTGSAGAVATGLNVGDVVVADTLVYHDVDL
                     TAFGYDYGQMSMQPLYFHSDKTFVSTFEAVLSKEEMTSKVGLIATGDSFIAGQEKIDV
                     IKGHFPQVLAVEMEGAAIAQAAQATGKPFVVVRAMSDTAAHDANITFDEFIIEAGKRS
                     AQVLMAFLKAL"
     gene            complement(3862..4131)
                     /locus_tag="VBE34_001621"
     CDS             complement(3862..4131)
                     /locus_tag="VBE34_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264324.1"
                     /translation="MGKPLLTDDMIERSNRGEKVSGQTILDQETKIISTEDGMEQLTD
                     ENGKHIYKSRRIENAKRNEFQRKLNLVLFILLILLALLFYAIFKL"
     gene            complement(4131..4685)
                     /locus_tag="VBE34_001622"
     CDS             complement(4131..4685)
                     /locus_tag="VBE34_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7264325.1"
                     /translation="MDFEEKTINRQTVFDGQIIKVAVDDVELPNGLGQSKRELVFHGG
                     AVATLAVTPEHKIVLVKQYRKAIEGISYEIPAGKLETGESGSKEEAALRELEEETGYT
                     GNLEILYSFYTAIGFCNEKIVLYLATDLQKVENPRPQDDDEVLELLELSYEDCMQMVE
                     KGMIQDAKTIIALQYYGLKMGGHS"
     gene            complement(4706..6085)
                     /gene="glmU"
                     /locus_tag="VBE34_001623"
     CDS             complement(4706..6085)
                     /gene="glmU"
                     /locus_tag="VBE34_001623"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEO7264326.1"
                     /translation="MSNYAIILAAGKGTRMKSDLPKVMHKVSGITMLEHVFRSVQAIE
                     PSKIVTVIGHKAELVRDVLGDKSEFVMQTEQLGTGHAVMMAEEELATSKGHTLVIAGD
                     TPLITGESLKNLIDFHVNHKNVATILTADAANPFGYGRIIRNSDDEVTKIVEQKDAND
                     FEQQVKEINTGTYVFDNQSLFEALKDINTNNAQGEYYLTDVIGIFKEAGKKVGAYKLR
                     DFDESLGVNDRVALATAEKVMRHRIARQHMVNGVTVVNPDSAYIDIDVEIGEESVIEP
                     NVTLKGQTKIGKGTLLTNGSYLVDAQVGNDVTITNSMVEESIISDGVTVGPYAHIRPG
                     TSLAKGVHIGNFVEVKGSQIGENTKAGHLTYIGNAEVGCDVNFGAGTITVNYDGQNKF
                     KTEIGSNVFIGSNSTLIAPLEIGDNALTAAGSTITDNVPIDSIAIGRGRQVNKEGYAN
                     KKPHHPSQK"
     gene            complement(6329..6742)
                     /locus_tag="VBE34_001624"
     CDS             complement(6329..6742)
                     /locus_tag="VBE34_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7264327.1"
                     /translation="MVKALETYIVTNGNGRQAVDFYKDVFQADLVNMMTWEEMDPNCL
                     EDRKDLIINAQLIFDGIRLQISDENPDFVYQAGKNVTAAIIVGSVEEAREIYEKLKKS
                     AQEVQLELQETFWNPAYANLVDQFGVMWQISTELN"
     gene            complement(6755..7132)
                     /locus_tag="VBE34_001625"
                     /pseudo
     CDS             complement(6755..7132)
                     /locus_tag="VBE34_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625774.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7221..8177)
                     /locus_tag="VBE34_001626"
     CDS             complement(7221..8177)
                     /locus_tag="VBE34_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7264328.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIEGIDLVAILSTVRSLETAKDLA
                     KEYNMSLATSEYKAVLDNEEIDTVYIGLPNHLHFDYAKEALLAGKHVICEKPFTLEAS
                     QLEELVSIANTRQLILLEAITNQYLPNFDLVKEHLSNLGDIKIVECNYSQYSSRYDAF
                     KRGEIAPAFNPEMGGGALRDLNIYNLHLVIGLFGEPITAQYLPNIERGIDTSGVLVLD
                     YGHFKTVCIGAKDCSAEVKSTIQGDKGSIAILGSTNTMPKISLTMNGQESHVYQLNGD
                     RHRMHDEFVIFEGIISNLDFKRAAQALEHSRTVMKVLDMVTK"
     gene            complement(8174..8626)
                     /locus_tag="VBE34_001627"
     CDS             complement(8174..8626)
                     /locus_tag="VBE34_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEO7264329.1"
                     /translation="MKAQELWNKFKSLNPSIGDEIDAWQFGAEPDLLAKLVVDGTKTA
                     TASAYDLYELDEEGLPQVGSYDIILDSNDEAICIIEITKVDIVPFKDVSADHAFKEGE
                     GDKTLEWWRKAHIDFFKPYFEEFGLMFSEDSRIVLEEFQVVYPKEINE"
     gene            complement(8623..8868)
                     /locus_tag="VBE34_001628"
                     /pseudo
     CDS             complement(8623..8868)
                     /locus_tag="VBE34_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233822.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3977 family protein"
     gene            complement(8865..9563)
                     /locus_tag="VBE34_001629"
     CDS             complement(8865..9563)
                     /locus_tag="VBE34_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEO7264330.1"
                     /translation="MTKVVLVTGCASGIGYAQAQYFLKQGYQVYGVDKSDKPNLNGNF
                     NFIKLDLSSDLSPLFTMVPTVDILCNTAGILDAYKPLLEVSDEELEHLFDINFFVTVR
                     LTRHYLRRMVEKKSGIIINMCSIASFIAGGGGAAYTSSKHALAGFTRQLALDYAKDCI
                     QIFGIAPGAVQTAMTASDFEPGGLAEWVASETPIGRWTKPSEVAELTGFLASGKARSM
                     QGEIVKIDGGWSLK"
     gene            complement(9556..9792)
                     /locus_tag="VBE34_001630"
     CDS             complement(9556..9792)
                     /locus_tag="VBE34_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEO7264331.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGDAENYVQLFDTLEVNGKVLQAT
                     PYFLINVTGEGEGFSMWAPTPCDVLAEDWIEVND"
     gene            complement(9882..10130)
                     /locus_tag="VBE34_001631"
     CDS             complement(9882..10130)
                     /locus_tag="VBE34_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264332.1"
                     /translation="MEKITNDFEKVIAVLNRLSISYELVENGPVLLTEQADNFIVGIE
                     GVRTKIMFLTNKKKTKYYLLIMDDQKLFYPMKTIVAFR"
     gene            complement(10328..10828)
                     /locus_tag="VBE34_001632"
     CDS             complement(10328..10828)
                     /locus_tag="VBE34_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7264333.1"
                     /translation="MACIIRRARLGDEVNLAYIQTESWKAALGKILPEDIIQKTTEIE
                     PAITMYQQLLHKEVGKGYILEVDSNPHCMAWWDKSREDGMLDYAELICIHSLKEGWGK
                     GYGSQMMNHVLSEIQQAGYNKVILWVFTENTRARKFYDRFGFSFKGKSKTYFEKEEMC
                     YEKILH"
     gene            complement(11068..>11307)
                     /locus_tag="VBE34_001633"
     CDS             complement(11068..>11307)
                     /locus_tag="VBE34_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEO7264334.1"
                     /translation="YCVLITLSSFLHRIYSQQLVIGILDIVKVFYIAVFRYLILHPVL
                     TFVKVASVIGYKNKKMVWGHITREKQMKDFETSVT"
BASE COUNT         3614 a         2344 c         1541 g         3808 t
ORIGIN      
        1 acaggatcaa aagaagtcaa taataattct ttaaagaaga tagtaatcag agtcagtaca
       61 attaatccca caataatagt catatattta tcactatctt gtactgccaa aatattccca
      121 aaaagaatat gaaagaggtc agttgtacta ttagcaagtc caattaaaat gatacctaag
      181 gctaaaaagg acgaaaaagt aattccaata gcagtatctc cttttatgac actattttct
      241 ttaatataag taatgataat cgaagacaac aaaccaaaga caatagcacc tataaaaaag
      301 ttaatcccta aaataaaaga tatagcaaca cctggtagaa cagcgtgaga aatagcatca
      361 cccatcaaag acattgagcg caagataata aaacagccta ctgcacccgc tacaatacca
      421 atcacaattg cagttataaa agcattttgc aaaaaatgat aagtcaataa gccttcaaaa
      481 aatttcgtaa acattctagt ctccttgtcc taacaaaata gcatcaccgt aagcagcact
      541 taaattctca cgggtaaatg cctgatctat tggtccacaa gcaattagat gacggttcaa
      601 aataattact tgatcaaaat aatgatcaac tttactcaaa tcgtgatgga cgactaaaat
      661 taattttccg gccttactta atttttttag taaattaact atgatttgtt cactaatgga
      721 atcaatccct acaaacggct catctaaaaa aatgtaatcc gcttcttgaa cgagacatct
      781 tgccataagc atacgttgaa attgtccacc tgataaggca ttaattggtc tattttcaaa
      841 ccctctcaaa ccaacttgat caatcactcg ggatactttt tcccagtcag tttttgatag
      901 tcgcttgaag aggcctcgtt ccttatataa tcctaaagaa acgcattctc caacagttat
      961 agggaattgg taatcaatat tagttttttg ttcaacatat gctactctcc ccattaaagg
     1021 tagcaaatct cccccaatcc ctgattctcc agtagaatca accaaaccaa gaagagcttt
     1081 cataagcgta gatttccccg ccccattagg ccctaaaata ccaatgattc cggagccttc
     1141 taagcgtaaa ttaatatctt ctagcactaa attattatca taacttacgc ttaagtgttt
     1201 actaataatc atttttcctc caaaagtaga atggagctgg ttataaaacc agactccact
     1261 tctctgtatt tattaaagct aagtcagttt ttagattagg atttcgacat tgttacaact
     1321 cagccttaag cgagaatact acatcattga tttcattatg atttcaatgt cgattctcac
     1381 tcttattttg ctaaaccttc tgcaatctta tcaagattcc atttcatcat agcgtagtag
     1441 ctgtcaccct tttgaccttt tttagcaatt gagtctgtga agatttctgc gtaaattggg
     1501 atacctgatt ctcttgaaac agatttcata ggacgcttgt caacacttga ttcaacgaat
     1561 aaagctgaag gtcttacttg ttttaatttc ttaactaagc tagtaatttg atctggtgta
     1621 ccttcttctt cagtgttaat ttcccaaatg tatgctgatg gaactccata agcttttgag
     1681 aaatatttaa agcaaccttc actagtaaca attaattttt tattagctgg aatggcatta
     1741 aatttagatt ttgcttcttt atccaatttt tctaatttag ctacataagc atctctgttt
     1801 ttctcataag tagctttgtt ttttggatct ttagcaatta attgcttagc aatatttttt
     1861 gagtaaataa taccattttc aaggttcaac caagcatgtg ggtcttcttt accttttccg
     1921 ctttgaccat ttagataaat aacatcaact ccatcgctaa ccgcaaagta atctttgttt
     1981 tctttcttct tagcgttctt tattaatttt gtaaaccaag cattaccgcc tgtttcaaga
     2041 ttaattccgt tgtaaaaaac aaggtctgct tgagttgttt tttcaacgtc tgctggtaat
     2101 ggctcatatt catgaggatc tttaccaaca ggtacaatac tatgtaagtc gattttgtta
     2161 ccggctatat tttttgttat atctgccaaa atagagttgg ttacaactac ttttaatttt
     2221 tcgtttttgg cttgatcctg cttgttagca caggcaccta agcctaaaaa tgctacaaaa
     2281 catgatacaa taactaacca ctttttcatc aaaatgctcc ttttaaaatt aatataagtt
     2341 atgtattaaa ttaagttaac ttaattttga tattagcata acctattata aagtatttgt
     2401 caaatacttt ttcagtattt tttgatataa tggtcttaga aaaatagatt ttaagaagta
     2461 taagaaagac tgaatatgac tccaaataaa gaagattacc taaaatgcat tcatgaatta
     2521 ggtgaaaaaa acaacaaaat ttccaataag aaaatcgctg aaatgatgca agtctcggct
     2581 cctgccgtgt cagaaatgat taaaaaaatg ataagtgaac aactgattgt taaagataaa
     2641 gatttaggat attatcttac caaacaaggt ttattagttg tttctgacct ttaccgtaaa
     2701 catcgtctag tagaggtatt tttagttaac catttgcact atactgctga tgatattcat
     2761 gaagaagcgg aagttttaga acatactgta tcaacaactt ttgttgatca attagaaaag
     2821 ttgttagact ttccacaatt ttgtccacac ggtgggacta ttcctaaaaa gggagagttt
     2881 ttagtagaaa ttaaccaaat gacattggac caaattagtc agcttggcac atatgttatc
     2941 agtcgtgttc atgatgattt tcagctactg aaatacctag aacaacaccg tttgcatatc
     3001 aacgatacca ttgaattgac acaaattgac ccttatgcaa aaacttatca catcacctat
     3061 aacgatgaaa atctaacaat ccctgagcgt atcgcaagtc aaatatatgt atctgctaca
     3121 taaataaaaa agtccgtgta ttcattagat acacgggctt ttttataatg ctttcaaaaa
     3181 tgccattaat acttgggcag agcgtttacc agcttcaatg ataaattcat caaatgtgat
     3241 attggcatca tgagctgcag tatcactcat agcacgtacc accacaaagg gctttcctgt
     3301 agcttgagca gcttgggcaa ttgctgcacc ttccatctcg acagctaaaa cctgtggaaa
     3361 atgcccctta ataacatcaa tcttttcctg gccagcaata aaactatccc cagttgcaat
     3421 taaaccaact ttactagtca tttcttcttt agataaaact gcttcaaaag tggaaacaaa
     3481 agtcttatcc gaatgaaaat aaagtggctg cattgacatt tgtccatagt cataaccaaa
     3541 ggcggttaaa tctacatcgt gataaactaa tgtatctgcg acaacgacgt ctcctacatt
     3601 caatcctgtt gctactgcac cagctgatcc tgtattaata atagcatcaa ctttaaagct
     3661 ttctactaaa atagcaacac tcatcgctga catgactttc ccaacaccag actgtactaa
     3721 aacaagctca tgttcaccat aacgtccact gtaatataca tttgataata ctgtctcttg
     3781 agatttatcc tcaagatttt ccacaaggag ttttaactct tcctccatgg ctgcaataat
     3841 tccaattttc atgatatttc cttataattt aaaaattgca taaaacaaga gagccaacaa
     3901 aataagcaaa atgaataaaa ctaaattcaa cttacgttga aattcatttc gttttgcatt
     3961 ctcaatccgg cgactcttgt agatatgttt accgttctcg tcagttagtt gctccatgcc
     4021 atcttctgta gaaataattt tagtttcttg atctaagatt gtttgacctg aaactttttc
     4081 acctcgattt gaacgttcaa tcatatcatc tgttaacaat ggttttccca ttaagaatga
     4141 cctcccattt ttaaaccgta atattgtaaa gcaatgatcg ttttcgcatc ttgaatcatg
     4201 cctttttcta ccatttgcat acaatcctca taacttaact ctagaagctc aaggacctca
     4261 tcatcatctt gtggcctagg attttcaact ttttgtaaat cagtagctag atagaggacg
     4321 attttctcat tacaaaaacc aatagcagtg tagaaactat aaagaatttc aagattccct
     4381 gtgtagccag tctcttcttc taattcccga agggcagctt cttccttaga acctgactca
     4441 cccgtctcaa gtttccctgc tggaatttcg taagaaatac cttcaatagc cttacgatac
     4501 tgtttcacta aaacaatttt atgctcagga gttaccgcca aagtagcaac agctcctcca
     4561 tgaaaaacaa gttcacgttt ggattgtccc aatccatttg gcaactctac atcatcaaca
     4621 gctactttga taatttgtcc atcaaaaaca gtttgacgat taattgtttt ttcttcaaaa
     4681 tccataagaa cctcccaaat aagtgttact tttgtgaagg atgatgtggt tttttattgg
     4741 catatccttc tttattgact tgacgtcctc ttccaatagc tatgctatca attggaacat
     4801 tatctgtgat ggtagatcca gctgctgtta aagcattatc accaatttca agtggtgcaa
     4861 tcagagtaga attacttccg ataaagacat tacttccgat ttctgtttta aatttatttt
     4921 gtccatcata attgacagta atcgtccctg cgccaaagtt aacatcacaa cctacctctg
     4981 cattgccgat ataagttaaa tgacctgcct tcgtattctc acctatttga gaacccttta
     5041 cttcaacgaa attaccaata tgaacacctt ttgcaagact tgttcctggt ctaatatgcg
     5101 cataagggcc tacggttact ccatcagaaa taatggattc ttcaaccatc gaattagtga
     5161 tggtaacatc atttccgact tgagcatcta ctaaataact accattggtt aataaagtac
     5221 ctttaccaat cttcgtctgt ccttttaaag tcacattagg ctcaattaca gattcttctc
     5281 caatctcaac atcaatatca atataagcgg aatcaggatt aaccactgtg accccattaa
     5341 ccatatgttg acgtgcaatt cggtgacgca tcactttttc agcagttgca agtgctacac
     5401 gatcattaac tcctagactt tcgtcaaagt cacgaagttt ataggcacct acctttttac
     5461 ccgcttcttt gaaaattcca ataacatcag ttagataata ctctccttga gcgttatttg
     5521 tattaatgtc tttaagtgct tcaaatagac tttgattatc aaaaacataa gttccagtat
     5581 taatctcttt aacctgctgc tcaaagtcat tagcatcctt ttgttctaca attttagtaa
     5641 cttcatcatc tgaattacgg ataatacgac catatccaaa tggatttgca gcatcggctg
     5701 ttaagattgt tgcaacgttt ttatgattga catgaaaatc aattaagttt tttaaacttt
     5761 ctccagtaat caggggtgta tcacctgcaa taactagtgt atgtcctttt gatgtcgcta
     5821 attcttcttc cgccatcatt acagcatggc cagttcctag ctgttctgtt tgcattacaa
     5881 actctgattt gtcacctaaa acatcacgta caagctccgc cttatgtcca ataacagtaa
     5941 caattttact tggctcaata gcttgcacac tacggaaaac atgctcaagc atggtaatac
     6001 cagatacttt atgcataact tttggtaaat cagatttcat gcgagttccc ttacccgcag
     6061 ctaaaataat ggcataattt gacatattaa ttcctctatc tctgaatata attcaaccta
     6121 ttatatcata atgccttcaa cttatcacta ataccccttt aaatccctat tacaatagac
     6181 tttagctaaa atagcttact tcaattagga ataaaatagc taccttttgg tagctattca
     6241 atacatgaaa tccgttaatt ttttcttctc tgtttgcaat cagtattttt ataaatctat
     6301 cattgcaatg ctcagatggt aaaaacgatt agtttaactc tgttgaaatt tgccacatca
     6361 caccaaattg gtctactaag tttgcgtaag caggattcca gaaagtttct tgtaactcta
     6421 actgaacttc ttgtgcactc ttctttaatt tttcataaat ttcacgcgcc tcctctacgc
     6481 tacccacaat aatagcggct gtaacatttt taccagcttg atacacgaaa tctggatttt
     6541 catccgagat ttgaagacga atgccatcaa aaataagctg agcattaatg attaaatcct
     6601 tgcggtcttc aagacagttg gggtccatct cttcccatgt catcatattc actaaatccg
     6661 cttgaaagac atctttataa aaatcaaccg cttgtcgccc atttccattt gttacgatgt
     6721 atgtttctag tgctttaacc ataagttctc ctttttattt tacttcacgg attgcaaagt
     6781 aattattgtc attatcggaa aaattaaaaa ctctcatact ccccatatca acaataggat
     6841 ttgtcataac ctcatttgct gttaattctt gatatgtact gtcaatatca gtagtttcaa
     6901 ataaaataga gggagttgct aaatctagct caggactcat ttgagctata attgctttgt
     6961 catgtaagac gaagctaact tcactatcca acttcggtgc aactatatag gttacataat
     7021 ctccttgtgt ctatttttca acacgctcaa aacccacctt gtttttccaa aaatctgctg
     7081 atgcttcaac attgctaacg tagagcatta cctgtccaat tgatgaaatc attttattat
     7141 ctcactttcg gttttattac taattagtat aacatacagt atttaaaata agtaaaaaat
     7201 gactcaaaag agtcatttac ttatttcgtt accatatcta gaactttcat caccgttcta
     7261 ctatgttcta aagcttgagc agcacgctta aaatctaaat tgctaataat accttcaaaa
     7321 ataacaaatt catcgtgcat gcggtgccta tcaccattta attgataaac atgactttct
     7381 tgtccattca tagttaatga aattttaggc atagtattag ttgaccctaa aatcgctata
     7441 gaccccttat caccttgaat agtagatttt acttctgcac tacaatcttt tgctccaata
     7501 cagactgttt taaagtgccc atagtctagg actagaactc ctgaagtatc aataccacgc
     7561 tctatatttg gtaaatactg tgctgttata ggttctccaa aaagtccaat tactaaatgt
     7621 aaattataaa tattaaggtc tctcaacgca cctccaccca tttcaggatt gaaagctggt
     7681 gctatttccc cacgtttaaa ggcatcataa cgcgatgaat attgggaata attacattct
     7741 acaattttaa tatctccaag gttgcttaaa tgttctttta caaggtcaaa attaggtaaa
     7801 tattgatttg taattgcttc gagtaatatt agttgcctag tattggcaat agatacaagt
     7861 tcttctaatt gacttgcttc taatgtaaat ggcttctcac aaatgacatg ttttcctgcc
     7921 aagagtgcct ccttagcata gtcaaaatgc aagtgattcg gcagtccaat atagactgtg
     7981 tcaatctcct cattgtccaa gacagcctta tattcactcg tagctagaga catgttatat
     8041 tccttagcta gatctttagc tgtctccaaa cttctaactg ttgataagat agcaactaga
     8101 tcaatacctt caattttctg caagacaggc aaaacttcct taacaatcat acctgtcccc
     8161 aaaaccgcta atttcattcg ttaatctcct ttgggtaaac cacttggaat tcctctaaaa
     8221 ctatcctaga gtcctcacta aacattaaac caaactcctc aaaataaggt ttaaagaaat
     8281 caatgtgagc ttttctccac cattctaaag ttttatctcc ttctccttct ttaaaagcat
     8341 ggtctgcaga gacatcttta aaagggacaa tatcaacttt agttatttca ataatacaga
     8401 tagcttcatc attgctatcc aaaataatat catatgaacc aacttgtggt aacccttcct
     8461 catctaattc atataaatca taggcagagg ccgttgcagt ttttgtccca tctacaacta
     8521 actttgccag aagatctggt tctgctccaa attgccatgc gtctatctca tcaccgattg
     8581 aaggattcaa agatttaaac ttattccata attcttgtgc cttcatatta cctccgaagc
     8641 aattcccaca acaaacttaa ctgcgttact catttttctt tgtcttttaa atccttcttt
     8701 aacatctaaa atataaacag tttgaccaat ccaaacacgt aaataaacag attttattaa
     8761 aatatcacct gatattcctt taacttcccg ctcagtacca tcagatgctt caaattccgt
     8821 acgtaataac caagtattac cacaaccaaa ttcaatctat tttctcattt caaactccag
     8881 ccaccatcaa ttttgacaat ttcaccttgc attgatctcg cttttcctga agcaagaaat
     8941 ccagttaatt cagcaacttc acttggttta gtccaacgac caattggcgt ctcactagct
     9001 acccattctg ctaatccacc aggttcaaaa tcactagcag tcatagcagt ttgtacagca
     9061 cctggcgcta taccaaaaat ttgaatacaa tcttttgcgt aatccaaagc caattgacgt
     9121 gtgaatcccg caagggcatg tttagaacta gtatatgcag ctccaccgcc acccgctata
     9181 aaacttgcta ttgagcacat attgataata atacctgact tcttttccac catacgtcta
     9241 agataatgac gtgttagcct aacagtcacg aaaaaattaa tatcaaatag gtgttccagt
     9301 tcttcatcac ttacttctag aagtggcttg taggcatcta gaatacctgc agtattacac
     9361 aaaatatcaa cagtcggtac cattgtgaat agtggggata aatcagagga taaatccaac
     9421 tttataaagt taaaattacc atttaagttc ggtttatccg acttatccac accatatact
     9481 tgataccctt gtttcaaaaa atattgagcc tgtgcataac caattccact agcacagccc
     9541 gtcactaaaa caactttagt cattgacctc gatccaatcc tctgctaaaa catcacatgg
     9601 tgttggtgcc cacatggaaa aaccttcacc ttcaccagta acattaataa gaaaataagg
     9661 tgtagcctgt agtactttcc cattaacttc aagggtatca aataactgaa cataattttc
     9721 agcgtccccc caacctgtac gtacatattt tttctttgct tttaaacctg gtaaaatttc
     9781 ttcaaatgtc atattgttct cctttattgt ctttaaccat tgatatatca aggtttattg
     9841 cttatttaaa atcataattt aaagtttctt cgccttttag ttcatctaaa ggctactatt
     9901 gttttcatag gataaaatag tttctggtca tccattatta gtaaataata tttcgtcttt
     9961 ttcttgttgg tcaagaacat tatttttgtt cttactcctt caattcccac aataaagtta
    10021 tctgcttgct ccgtcaacaa aacaggtcca ttttccacta attcataaga aatacttagt
    10081 cgatttagca cagctataac tttttcaaaa tcattcgtaa ttttctccat tcttccatag
    10141 agttagcata actatttgta gactagagaa cctaaaattg atacactgtt tttcactcaa
    10201 aaaattttac cttcataatc ttcattatac ctataacaat tttcttttac aaatttgttc
    10261 cccttaaagg aaattgtttt tcaaaatagg atgtttatac tattgtatta tcggatgctt
    10321 tatctcgcta gtgcaaaatc ttctcataac acatttcttc cttttcaaaa taagtttttg
    10381 atttaccctt aaaagaaaaa ccaaacctat cgtaaaattt ccttgcgcgt gtattttctg
    10441 taaataccca aagtatcact ttattataac ctgcttgttg tatctctgat agtacatgat
    10501 tcatcatttg agacccataa ccttttcccc aaccttcttt aagactatgt atacaaatga
    10561 gttctgcata atcaagcata ccatcttctc tacttttatc ccaccaagcc atacaatgag
    10621 gattgctatc tacttccaat atatacccct taccaacctc cttgtgtagt agctgttgat
    10681 acattgttat ggcaggttct atttccgtag ttttttgaat gatatcctca ggtagtatct
    10741 tacccaaagc agctttccag ctctccgttt gaatataagc taaattgact tcatctccta
    10801 atcttgctct cctgattatg caagccataa ctaaaatcct ttcaaaaatt attattaact
    10861 gcctgataac tactccaatt ataccatttt cgtgatatta atttttattt tgaacagttc
    10921 tggttctcat ttatcattat atttctaaaa ttttagatgg ttttaaacat taacctcaga
    10981 ttaggccaat tattgataat cactaaaaga gcgtagataa ttacgctctt ttagtgatta
    11041 ttttatatat atgaatcgct ataacttcta agtaacactt gtttcaaaat ccttcatttg
    11101 tttctctctc gtaatatgcc cccaaaccat ttttttattt ttatagccaa taacacttgc
    11161 aaccttaaca aaagttaata caggatgtag gataagatac ctaaaaacag caatataaaa
    11221 tactttaaca atatctaaaa ttcctattac tagttgctga gaataaatac ggtgtaagaa
    11281 agagcttaat gtaattaaaa cacagta
//
LOCUS       DATRUK010000062        10903 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.062, whole genome shotgun sequence.
ACCESSION   DATRUK010000062 DATRUK010000000
VERSION     DATRUK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10903
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.062"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(26..712)
                     /locus_tag="VBE34_001634"
     CDS             complement(26..712)
                     /locus_tag="VBE34_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HEO7264335.1"
                     /translation="MIEFFSNIRTEIPQMPLLIHSLILSVLPFLMWLTLVNRDKPLYK
                     TIWSILLGLQLITIYTWFFWAKLPLSESLPLYHCRIGMFVVLLARPRILKDYFALLGV
                     VGGVLAMIHPDFYPYQFLHVTNIFFFIGHFALFVLSLLHLMTQSNLDKLNPKLIIQLT
                     LLINMSLIFINLLTGGNYGFMMKTPILGITNPFLNLLIVTTLLSFLVLFVKQIFQKVR
                     MRASFKTEES"
     gene            complement(841..2061)
                     /locus_tag="VBE34_001635"
     CDS             complement(841..2061)
                     /locus_tag="VBE34_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEO7264336.1"
                     /translation="MKNLNRYVVAVSGVVLHLMLGSTYAWSVFRNPIISETGWDISSV
                     SFAFSLAIFCLGMSAAFMGHLVERFGPRIMGMISAILYGAGNVLTGLAIETQQLWLLY
                     VAYGILGGIGLGSGYITPVSTIIKWFPDRRGLATGFAIMGFGFASLVTSPLAQSLLIR
                     IGVGKTFYILGLVYFFVMMIASQFIKQPPQEKITILTHDGKKNAMNSQIITGLKANVA
                     IKSKTFYIIWLTLFINISCGLGLISAASPMAQDLAGYSAESAALLVGVLGIFNGFGRL
                     LWASLSDYIGRPLTFIILFIVNFIMTSSLFLSFNAIVFAIAMSILMTCYGAGFSLLPA
                     YLSDIFGTKELATLHGYSLTAWAIAGLFGPLLLSKTYSWGNSYQLTLMVFGFLFLFGL
                     LLSLYLRKLTTKVV"
     gene            complement(2248..3615)
                     /locus_tag="VBE34_001636"
     CDS             complement(2248..3615)
                     /locus_tag="VBE34_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEO7264337.1"
                     /translation="MKLSLHEVAKVVGAKNQVSEFEDVPLGNIEFDSRNISEGDLFLP
                     LKGARDGHEFIEMAFDNGAIATISEKEIAGHPYLLVSDALKAFQVLAQYYIEKMNVDV
                     IAVTGSNGKTTTKDMIAAILSTTYKTYKTQGNYNNEIGLPYTVLHMPEDTEKIVLEMG
                     QDHLGDIHVLSEIAKPRIAVVTLIGEAHLEFFGSREKIAEGKMQITDGMSSDGILIAP
                     GDPIIDPYLPANQMTIRFGHDQELQVTELKEEKHSLTFKTNALEHQLRIPVPGKYNAT
                     NAMVAAYVGKLLAVAEEDIVDALENLQLTRNRTEWKKSANGADILSDVYNANPTAMRL
                     ILETFSAIPNNDGGKKIALLADMKELGEQSVDLHNQMIMSIRPDSIDTLICYGQDIEG
                     LAQLASQMFPIGKVYFFKKNQEVDQFDQLLAKVKDTLKEKDQILLKGSNSMNLSKIVD
                     ILEVG"
     gene            complement(3762..4808)
                     /locus_tag="VBE34_001637"
     CDS             complement(3762..4808)
                     /locus_tag="VBE34_001637"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEO7264338.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIT
                     QVGQFIKTQEFDEMPSSDEKLMTNQTVDLDKMVRPSDIYDDNAIVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKQVLATVGVPQVAYQTYFEGDDLEHAIKL
                     SLETLSFPIFVKPANMGSSVGISKATDESSLRSAIDLALKYDSRILIEQGVTAREIEV
                     GILGNNDVKTTFPGEVVKDVDFYDYDAKYIDNKITMDIPAKVDEATMEAMRQYASKAF
                     KAIGACGLSRCDFFLTKDGQIFLNELNTMPGFTQWSMYPLLWENMGLTYSDLIEKLVM
                     LAKEMFEKRESHLI"
     gene            complement(4949..5545)
                     /gene="recR"
                     /locus_tag="VBE34_001638"
     CDS             complement(4949..5545)
                     /gene="recR"
                     /locus_tag="VBE34_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEO7264339.1"
                     /translation="MLYPTPIAKLIDSFSKLPGIGTKTATRLAFYTIGMSDEDVNEFA
                     KNLLAAKRELTYCSVCGNLTDDDPCLICTDKTRDQSVILVVEDSKDVSAMEKIQEYNG
                     LYHVLHGLISPMNGISPDDINLKSLITRLMDGQVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            complement(5560..7605)
                     /locus_tag="VBE34_001639"
     CDS             complement(5560..7605)
                     /locus_tag="VBE34_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEO7264340.1"
                     /translation="MLNRKKRYRLTVKKQNASIPRRLNLLFFIIVLLFTVLILRLEQM
                     QIGQQSFYMKKLTALTSYTVKESKARGQIFDAKGVVLVENDERPTVAFSRGNNISSQS
                     IKELANKLSHYITLTEVASSDRAKRDYYLADKANYKKVVESLPDSKRYDKFGNHLAES
                     TVYANAVAAVPVSAINYSEDELKVVALFNQMNATPTFGSVKLSTGELSDDQIKKLDAD
                     KKELLGISVTSNWHRRKKGTSLSDILGTISTEKAGLPREEVKKYLKKGYSLNDRVGTS
                     YLEKQYEDDLQGIRQIRKVVVNKKGKVVSDNITQEGKSGRNLKLTIDLNYQNKVESIL
                     KQYYGSELSSGRASFSEGMYAVAIEPSTGKVLAMAGLKNDHGNLVDDSLGTIAKNFTP
                     GSVVKGATLSSGWENKVLRGNEVLYDQEIANIRSWFTRGLTPISAAQALEYSSNTYMV
                     QVALRLMGQDYNTGDALTDRGYQEAMAKLRKTYGEYGLGVSTGLDLPESEGYVPGKYS
                     LGTTLMESFGQYDAYTPMQLGQYISTIANNGNRLAPHVVSDIYEGNDSNKFAQLVRSI
                     TPKTLNKIAISDQELAIIQEGFYNVVNSGSGYATGTSMRGNVTTISGKTGTAETFAKN
                     INGQTVSTYNLNAIAYDTNRKIAVAVMYPHVTTDTTKSHQLVARDMIDQYISQFTGQ"
     gene            complement(7734..8426)
                     /locus_tag="VBE34_001640"
     CDS             complement(7734..8426)
                     /locus_tag="VBE34_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEO7264341.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIQ
                     AAGIEFDLAFTSVLKRAIKTTNLALEAADQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPDMAKDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKQLSDDEIMDVEIPNFPPLVFEFDE
                     KLNLVSEYYLGK"
     gene            complement(8603..9361)
                     /locus_tag="VBE34_001641"
     CDS             complement(8603..9361)
                     /locus_tag="VBE34_001641"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEO7264342.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFIEAVASKLPSSELVEAGIAAP
                     ALTLSTVLEAAKGSELKIAAQNSYFENSGAFTGENSPKVLAEMGTDYVVIGHSERRDY
                     FHETDQDINKKAKAIFANGLTPIICCGESLETYEAGKAVEFVGAQVSAALAGLSEEQV
                     SSLVIAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQAVADKVRVQYGGSVKPE
                     NVAEYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(9542..10738)
                     /gene="tuf"
                     /locus_tag="VBE34_001642"
     CDS             complement(9542..10738)
                     /gene="tuf"
                     /locus_tag="VBE34_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEO7264343.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPTSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDEKYEDIIMELMSTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEVEIVGIKEDIQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVLAKPGSINPHTKFKGEVYILSKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIEVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
BASE COUNT         3467 a         2154 c         1745 g         3537 t
ORIGIN      
        1 agataatata ataatattaa aataattaag attcttctgt tttaaaacta gctctcattc
       61 tcactttttg gaaaatttgt ttaacaaata ggacgagaaa acttaataga gttgtcacta
      121 tcaaaaggtt taaaaaggga tttgttattc ctagtattgg tgtcttcatc ataaagccat
      181 aatttccccc agtcaagagg ttaataaaaa tcaaagacat attaatgaga agcgttagtt
      241 gtataatcaa ttttggattt aatttgtcca aattggactg tgtcatcaga tgtagtaaac
      301 taaggacaaa taaagcaaaa tgtccaataa agaagaaaat attagtcaca tgtagaaact
      361 ggtaagggta aaagtctggg tgaatcattg ccaatactcc acctactact cctaacaatg
      421 cgaaataatc ctttaatatt ctaggcctcg ctaagagaac gacaaacatg cctattcgac
      481 aatggtaaag gggaagactt tcagataaag gcaattttgc ccagaaaaac caagtataaa
      541 tcgtaattaa ctgaagtcct aaaaggatac tccaaatagt tttatacaaa ggcttatctc
      601 tattaaccaa agtcagccac atcagaaaag gtaagacaga taaaatcaaa ctatggataa
      661 gtaaaggcat ctgcggaatc tctgttctga tattagaaaa gaattctatc attttagtat
      721 tatctttcta tcattttttt actagatact tagtatatca aattatttgc tataagacaa
      781 aactattttt caaagaaaaa actctttggt aaaaccaaag aattttaaat tagatatata
      841 ctacacaact ttagttgtta attttcttaa atatagagat aacaataatc cgaataagaa
      901 taaaaaacca aaaaccatta atgtcaattg ataggaattt ccccatgaat atgtctttga
      961 taacaatagg ggcccaaaca gacctgctat tgcccatgct gttaaactat aaccatgtaa
     1021 agtagctaat tcctttgttc caaaaatatc acttagataa gcaggtaata aggaaaaacc
     1081 tgcaccataa caagtcatta aaatagacat cgctattgca aatacaatag cattgaatga
     1141 caaaaataaa ctagaagtca taataaagtt cacaataaat aatattataa aggtcaacgg
     1201 gcgtccaatg tagtcagaga gacttgccca taacagacgt ccaaaaccgt taaatatccc
     1261 tagtacccct actaataagg ctgcagattc tgcggaatag cctgctaaat cttgtgccat
     1321 tggtgaagct gctgatatta aacctaagcc acacgaaata ttaataaaca aggtcaacca
     1381 aatgatgtaa aaggtttttg attttatagc gacgtttgct tttaatccag tgataatttg
     1441 tgaattcata gcattctttt taccatcgtg agtcaaaata gttatttttt cctgaggtgg
     1501 ttgtttaata aattgtgagg caatcatcat gacaaaaaaa tatactaatc ccaaaatata
     1561 aaacgtttta cccacaccaa tcctaatcag taaggattgt gcaagcggac ttgttactaa
     1621 agaagcaaag ccaaatccca taatagcgaa tcctgttgct agtcccctcc tatcaggaaa
     1681 ccatttaata atagtcgata ctggagtaat ataacctgaa ccaagtccga ttcctcctaa
     1741 aataccgtat gcaacataca gtaaccataa ctgctgagtt tcaatggcta agcctgttaa
     1801 cacattccct gctccatata aaatagcaga aatcattccc attatcctag gaccaaaacg
     1861 ctctactaag tgtcccataa aagctgcaga cattcctaga caaaaaatag ccaaactaaa
     1921 agcgaatgaa actgatgaaa tatcccaacc agtctctgag ataattgggt tacgaaacac
     1981 actccaagca taagttgatc ctagcattaa atgtaaaacg actccagaaa ccgcaactac
     2041 ataacgattt aaatttttca aataatcctc ctaaaaacga acttaagttt tatttttaaa
     2101 aatatagttc gcaataaaca gattatcaaa aaactgaata tatcaccagt tttctgctta
     2161 gcatatctat tttcctcaat aaaactgaat attattcgtg attaaaaaac aaaaaagtta
     2221 gggtaacttg ttaccctaac ccctctatta tcctacttca agaatatcaa ctattttaga
     2281 aagattcatt gagttagaac cttttaataa tatttgatct ttctccttaa gagtatcttt
     2341 tacttttgca agcaattgat caaactgatc cacttcttga ttctttttaa agaaatacac
     2401 tttaccaatt gggaacatct ggcttgctaa ttgagctaac ccctcaatat cctggccata
     2461 acaaatcaaa gtatcaatag agtcaggtct gatactcata atcatttgat tatgtaaatc
     2521 cacagattgt tctcccagtt ctttcatatc agctaaaaga gcaatcttct ttccaccatc
     2581 attattggga atagctgaaa aggtttctag tatcaatcgc atagccgttg ggttagcatt
     2641 gtaaacatct gataaaatat ctgctccatt ggctgatttc ttccattcgg tgcgattacg
     2701 agttagttga agattttcta gagcatccac tatatcttct tctgctactg ctaataattt
     2761 tccaacatag gcagcgacca tcgcattagt tgcattgtac ttaccaggta ctggtatacg
     2821 taattggtgc tccaaagcat tagttttaaa agttaaggaa tgtttttctt ctttgagttc
     2881 tgtcacttgc aattcttggt catggccaaa acgaatagtc atctggtttg ctggaagata
     2941 tggatcaata attggatctc ccggtgcaat taatatgcca tctgaactca taccatctgt
     3001 gatttgcatt ttcccttctg caatcttttc tcgactacca aaaaattcta agtgtgcttc
     3061 accaatcaaa gtaacaacag caatacgtgg ttttgcaatt tcagataaca catggatatc
     3121 tccaagatga tcctgtccca tctccaaaac aattttttca gtatcttctg gcatgtgtag
     3181 cacagtatat ggtaagccaa tctcgttatt gtagtttcct tgtgttttat aagtcttata
     3241 tgtggttgat aagattgcag ctatcatatc tttagttgtt gtcttaccat ttgagccagt
     3301 tactgcaata acatcaacgt tcatcttttc aatataatac tgtgccaaga cttgaaatgc
     3361 tttaagtgca tcagacacta aaagataagg atgacccgct atttccttct ctgaaatggt
     3421 tgctattgct ccattatcaa aagccatctc tataaactca tgtccatctc ttgctccttt
     3481 taagggaaga aataagtcac cttcactgat attacgacta tcaaattcaa tatttcccag
     3541 gggaacgtct tcaaactctg atacttgatt tttagcgcca actactttag caacttcatg
     3601 caaactaagc ttcatatcat ttccttttct attatttagc aacaatgttg tagatagtat
     3661 gctatccctt ataaacgaat atactcctaa attataacac actttcataa catatcatag
     3721 atttgaaaaa gctcaactaa gaaagactta gttgaacttt tttaaattaa atgactctca
     3781 cgtttttcaa acatttcttt agctaacata actaactttt caatcaaatc actataagtt
     3841 agccccatat tttcccaaag cagaggatac attgaccact gagtaaaacc gggcattgta
     3901 ttcagttcgt ttaagaagat ttgtccatct ttcgtcaaaa agaaatcaca gcgtgataaa
     3961 ccacaagccc cgattgcttt aaaagcttta cttgcatatt gacgcattgc ttccatagtt
     4021 gcttcatcaa ctttagctgg aatatccata gtaattttat tatcaatata tttggcgtca
     4081 tagtcataga aatcgacgtc tttaacaact tcgccaggaa aagttgtctt aacatcatta
     4141 ttgcctaaaa tacctacttc aatttcacga gctgtcacgc cttgttcaat caaaatacgg
     4201 ctatcatact tgagagctaa gtcaattgca gagcgaagtg aggattcatc tgtcgctttt
     4261 gaaataccta ctgatgaccc catattagcc ggttttacaa aaattgggaa acttaaagtt
     4321 tctaaagaga gtttaatcgc atgttccaaa tcatcaccct caaaataagt ttgatatgca
     4381 acctgaggta cacctactgt tgcaaggact tgttttgttg taattttatc catagccacg
     4441 cttgaagata gaatattagt cccaacataa ggcatcctta aaacttctaa aaatccttgg
     4501 atagaaccat cttcccccat tggtccatgt aaaacgggga aaacaattgc attatcatca
     4561 tagatatcac ttggacgaac cattttgtct aaatcaacag tttggtttgt cattaacttt
     4621 tcatctgaag atggcatttc atcaaattct tgtgttttaa taaattgacc tacttgcgtg
     4681 ataaaataag ttttaacaaa aaatttatca taattaatag cacgcatgac gctttctgca
     4741 gacagtacag atacttcacg ttccgcagag cgaccaccgt aaagcaagat caatgtttct
     4801 tttgacatat ttcttctctt ttctatctaa tctaagattg accttttaat aataccatat
     4861 tttaacttaa ctttcaaaaa tcaatccact taaagttagt attgtaaaag aaaaagttag
     4921 gagattccca accttttctt tataacaatt ataactctgt acgattttcg attgctcgaa
     4981 gcaaggttac ttcatctgca tattcgatat ctgaaccaac agctaaacct ctcgctaatc
     5041 gtgtcacttt gataccagct ggttttaaga ctcgtgaaat atacattgat gtagcttcac
     5101 catcagcagt cgcattggta gcaacaataa cttctgtcac ctgaccatcc atcaatcgag
     5161 taattaaact tttcaaatta atgtcatcag ggctaatacc attcataggt gaaattaagc
     5221 catgtaagac atgatacaaa ccattatact cttgaatttt ttccatagct gacacatctt
     5281 tactatcttc aacaaccaag ataacagact ggtcacgcgt cttatctgta caaattaagc
     5341 aaggatcatc atcagtcaaa ttaccacaaa cagaacaata ggttaattcc cgcttagcag
     5401 ctaataaatt cttagcaaat tcattaacat cttcatcact cataccaatg gtgtaaaaag
     5461 ctaaacgtgt tgctgtttta gtacctatac ccggtaattt tgaaaagctg tcaatcaatt
     5521 tagcaattgg tgttgggtaa agcattcaaa tgtcctctct tattgtcctg tgaactgtga
     5581 aatatattga tcaatcatat ctcgtgcaac taattgatgg gattttgttg tatcagttgt
     5641 aacatgcgga tacattaccg ctactgctat tttacgatta gtatcgtagg caatagcgtt
     5701 taagttgtag gtagaaactg tttgtccatt tatattttta gcaaatgttt cagcggtacc
     5761 agttttacca ctaatggttg tcacattccc cctcattgac gtaccagttg catagccact
     5821 tccactattg acaacgttat aaaaaccttc ttgaataatg gctaactctt gatctgagat
     5881 agctatctta tttagtgttt taggagtgat tgaacgaacc aattgagcga acttattaga
     5941 atcattccct tcatagatat ctgaaaccac gtgaggtgct aaacgattcc cattattcgc
     6001 aatagttgag atatactgac caagttgcat tggtgtatag gcatcatact gaccgaacga
     6061 ttccattaga gttgttccta agctgtattt tccaggtaca taaccttctg attcaggtaa
     6121 atctaatcct gtagaaaccc ctaaaccata ttcgccgtaa gttttcctta gtttagccat
     6181 tgcttcttga tagcctcgat cagttaaagc atctcctgta ttatagtctt gtcccattaa
     6241 acgaagtgct acttgaacca tataagtatt agatgaatac tctagcgctt gtgccgcaga
     6301 tattggagtc aaacctcgtg taaaccatga ccgtatatta gcaatttctt ggtcataaag
     6361 cacttcattt cctctaagaa ctttattttc ccatcctgat gatagcgtcg cacctttcac
     6421 aacagatcct ggcgtgaaat tcttagcaat agtgcctagg ctgtcatcaa ctaaattccc
     6481 atgatcattt ttcagcccag ccattgctaa cactttacca gttgaaggtt ctatagcaac
     6541 tgcatacatc ccttcggaaa aactagcgcg cccactagat aattcactgc catagtattg
     6601 tttcaagata ctttctactt tgttttggta attaagatca atcgttaatt taagatttct
     6661 tcctgactta ccttcttgcg taatattatc tgaaacaact ttaccttttt tattaacaac
     6721 tactttgcga atttgtctga ttccttgtaa gtcatcttca tattgttttt caaggtaaga
     6781 agttcccact cggtcattca gtgaatagcc ttttttcaaa tattttttaa cttcttctct
     6841 tggtaaacct gccttttctg tagaaatagt acctaatata tccgataaag aagttccttt
     6901 tttacgacga tgccaattac ttgttacaga aattcctaac agttccttct tatcagcatc
     6961 cagtttcttg atttgatcat cacttaattc acctgtactt aatttcactg aaccaaacgt
     7021 tggtgtcgca ttcatttggt taaataaggc aaccactttt aactcatctt cagaatagtt
     7081 aatagcgctc actggaactg cagcaactgc atttgcatat acggttgatt cggctagatg
     7141 gttaccaaat ttatcgtaac gtttagaatc tggtaaactt tcaacaactt ttttgtagtt
     7201 ggctttatct gctaaataat aatctctctt tgctctatcg cttgaagcaa cttcggttaa
     7261 ggtaatgtaa tgtgataatt tattcgctaa ctctttgata gactgagatg aaatattgtt
     7321 tcctctagaa aacgccacag taggacgttc atcattttca actaagacaa ctcccttagc
     7381 atcaaaaatc tgacctctcg cttttgattc tttcactgta taactcgtaa gagctgtcag
     7441 ttttttcatg taaaatgatt gttgcccaat ctgcatttgc tctaaacgaa gaattagaac
     7501 agtaaataat aaaacaataa taaaaaatag aagatttaat cgtctaggta tgctagcatt
     7561 ttgctttttt acagtgagac gatacctttt tttacgattc aacattaact ccttgctata
     7621 aaaactctga ctactattgt atcataaaaa tggtttttta cagacactca aaatgttata
     7681 aataataaaa aattcaagcc aacttggcct gaatttttta tataaagatt agattattta
     7741 cctaagtaat attctgaaac aaggtttaat ttttcatcaa attcgaaaac aagtggtggg
     7801 aagttaggaa tttcaacgtc catgatttca tcatctgaca attgtttgat atgttttaca
     7861 agagcacgga ttgagttacc gtgtgcacca acaaacacat ttttaccatc tttaagagca
     7921 ggagcaattt tatcttccca gaaaggaaga gcacgctcta aagtaacttt taggttttct
     7981 gcatctggaa taacagaatc atctagtgaa gcatagcgac gatcagtatg tgctgaatgt
     8041 tcatcatctt tagccatatc tggaggcaat acatcatatg aacgacgcca aatatgaact
     8101 tgctcatcac caaattgttc agctgcttct gctttatttt ttcctgtcaa tccaccgtaa
     8161 tgacgttcgt tcaagcgcca tgatttttca actggtaccc aaagttgatc agctgcttca
     8221 agggcaaggt tagttgtttt gatggcacgt ttaagaactg atgtaaaagc aaggtcgaac
     8281 tcaatacctg ctgcttgaat taatttccca gcatcaatag cttgttgtgt acctttttct
     8341 gaaagatcta cgtcagccca tccagtgaaa aggttagctt tattccactc agattcaccg
     8401 tggcgtgcga atactaattt taccattaga aaatttctcc tttaattttg gctataccaa
     8461 tatttacttt aactattcta caaaaaatag ccataaaaat ctagtctgat tactagttca
     8521 ataatcaaaa atagcttatt attaactatc tataagaaaa ggcatatcac ctagagtgct
     8581 atgcctttag atatcatgtg atttatttaa caaaatctaa taaggcaagg aagctttctg
     8641 cttcaagtga agctccacct acaagggcac cgtcaacgtc tgggcaagcc atatattctg
     8701 caacgttttc aggtttaact gaaccaccgt attgaacgcg aactttgtca gcaactgctt
     8761 gaccgaagtc tgctgcaaca acgtcacgaa ctgctttaca catgttttgt gcgtcgtctt
     8821 gagttgctga tttaccagta ccaatagccc agattggttc gtaagcgata acaagtgatg
     8881 atacttgctc ttctgacaaa cctgcaagag ctgctgaaac ttgagcacct acgaattcaa
     8941 ccgctttacc agcttcgtaa gtttcaagag actcaccgca acaaatgatt ggagtcaaac
     9001 cattagcaaa gatagctttt gcttttttgt tgatatcttg gtcagtttca tggaaataat
     9061 cacgacgttc tgagtgaccg ataacaacgt agtctgtacc catttcagcc aatactttag
     9121 ggctgttttc accagtgaat gcacctgaat tctcaaaata gctattttga gcagcgattt
     9181 tcaattctga acctttagct gcttcaagaa ctgttgataa agtaagtgct ggagcagcaa
     9241 tccctgcttc aacaagttct gatgatggta atttagaagc tactgcttcg atgaatgctt
     9301 ttgcttcttc tggattttta ttcattttcc agttaccagc gataaatggt ttacgtgaca
     9361 ttacacatac ctcttctttt tttatttact aatctatcat accataaatt atagttaatt
     9421 aaaagtttca cgagtctagt aaaacgcttt aattatagat actaaaaaag agtccctgag
     9481 gactcttcac aatttgagct gtctgactct aactgccgtt tgggaactta attaagttaa
     9541 attaagcttc gatttctgaa acgatacctg aaccaacagt acgtccacct tcacggattg
     9601 agaaagtagt accttgttct acggcgattg ggtgaatcaa ttcaacttcg atagtaacgt
     9661 tatcaccagg cataaccatt tctgttcctg ctggaagttc gattgaacct gttacgtcag
     9721 ttgtacggaa gtagaattgt ggacggtagt tgttgaagaa tggagtatga cgtccacctt
     9781 cttctttaga aaggatgtaa acttcacctt taaatttagt gtgtgggttg attgaacctg
     9841 gtttagcaag aacttgacca cgttcgattt catcacgttg aacaccacga agaagaacac
     9901 caacgttgtc ccctgcaaga ccttcgtcaa gttgtttacg gaacatttca acaccagtaa
     9961 caactgcttt ttggatatct tctttaatac caacgatttc aacttcgtcg ttgacacgaa
    10021 cagtaccacg gtcgatacgt cctgaagcaa ctgtaccacg tccagtgatt gagaatacat
    10081 cttcgactgg aagaagtaaa ggtttgtcag tatcacgttc tggttctgga atgtactcat
    10141 caacagtgct catcaattcc atgatgatgt cttcgtattt ttcatcgcct tcaagtgctt
    10201 taagagctga accttggata actggaaggt catcacctgg gaagtcgtat tctgaaagaa
    10261 ggtcacgaat ttccatttca accaattcaa gcaattcttc atcatcaaca aggtcaactt
    10321 tgttcatgaa tacgataagg tgtttaacac caacttgacg tgaaagaagg atgtgctcac
    10381 gagtttgtgg cattggtcca tcagttgaag caactacaag gatagctccg tccatttgag
    10441 cagcaccagt aatcatgttt ttaacgtagt ccgcgtgtcc tggagcgtcg atgtgggcat
    10501 agtgacgttt ttcagtttcg tactcaacgt gtgcagtgtt gattgtgatt ccgcgttcgc
    10561 gctcttctgg agcagcatcg atagaagcgt aatcttttgg ttggttaact gaagtaggca
    10621 agcgacgtgc caatacagtt gtgatagctg ctgttaaagt agttttaccg tggtcaacgt
    10681 gtccgattgt accaatgtta acgtgtggtt tactacgatc gtatttttct tttgccattt
    10741 taggaaaagc ctccaataaa atatatttta tagatagacg gtaggcaata cagtctaact
    10801 ttacttatct attttaacaa aatcctctaa aaaagcaagt attttatgct tttttgctta
    10861 tttctttatt tccttaaaat tcgggtaaga ttgattgagg cgt
//
LOCUS       DATRUK010000063        10736 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.063, whole genome shotgun sequence.
ACCESSION   DATRUK010000063 DATRUK010000000
VERSION     DATRUK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10736
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.063"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            313..1482
                     /locus_tag="VBE34_001643"
     CDS             313..1482
                     /locus_tag="VBE34_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO7264344.1"
                     /translation="MAWLGNFFTGASFSLVMPFMVLYVEQLGAPSNKVEWYAGLSVSL
                     SALSSALVAPLWGRLADKYGRKPMMVRAGLMMTFTMGGLAFIHSVTGLLILRILNGIF
                     AGYVPNSTALIASQAPQEESGYALGTLATGVTGGMLIGPLLGGLLAEWFGIREVFLLV
                     GTILLISTLMTIFMVKEDFKPISNEETMPTTEVFKSVKSLQILIGLFVTSMIIQISAQ
                     SIAPILTLYIRHLGQTENLMFVSGLIVSGMGFSSILSSPKLGRIGDRIGNHRLLLLAL
                     LYSFLMYVLCSLAQTSLQLGVIRFLYGFGTGALMPSINSILTKIAPRQGLSRIFSYNQ
                     MFSNLGQVLGPFVGSAVSIHLGFRWVFFVTSFIVLANFVWCFINFRKYIRVKEIV"
     gene            1479..1625
                     /gene="rpmG"
                     /locus_tag="VBE34_001644"
     CDS             1479..1625
                     /gene="rpmG"
                     /locus_tag="VBE34_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO7264345.1"
                     /translation="MRVKINLKCSSCGSKNYLTSKNKSNHPDKIEVPKYCPKERKVTL
                     HIES"
     gene            1672..1908
                     /gene="secG"
                     /locus_tag="VBE34_001645"
     CDS             1672..1908
                     /gene="secG"
                     /locus_tag="VBE34_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEO7264346.1"
                     /translation="MYNLLLTILLVLSVLLIISIFMQPQKNPSSNVFDSSGSEALFER
                     SKARGFEAFMQRFTGVLVFFWLLIGLVLSILSSH"
     gene            2021..4426
                     /gene="rnr"
                     /locus_tag="VBE34_001646"
     CDS             2021..4426
                     /gene="rnr"
                     /locus_tag="VBE34_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEO7264347.1"
                     /translation="MKEKIIKYLEEHGKTSVNDLAAALDMAGAKAFPKLIKTISNLES
                     HRQLRFDDNGSLSLQKKEAKKKEITVRGLFRANKAGFGFLSIDQDEDDMFIGKNDIAY
                     AIDGDTVEAVVKKPADRLNGTAAEARVVNIVERSLKTLVGKFVLDDERPKYAGYIKSK
                     NQKINQKIYIRKEPVVLDGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVLE
                     VLESMDIVSEFPEDVIAEANAIPDAPTEKDLIGRVDLRQEVTFTIDGADAKDLDDAVH
                     IKLLDNGHFELGVHIADVSYYVTEGSALNREALSRGTSVYVTDRVVPMLPERLSNGIC
                     SLNPNLDRLTQSCIMEIDQNGRVVNHQITQSVINTTYRMTYIAVNDIIAGDEEICSEY
                     ESIVSSVQHMVTLHHTLEAMRTRRGALNFDTSEAKIMVNDKGMPVDIVIRNRGIAERM
                     IESFMLAANETVAEHYARLKLPFIYRIHEEPKAEKLQKFIDYASVFGVQIQGTATKIT
                     QSALQDFMKKVQGQPGSEVLSMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRRY
                     PDLLVHRMIRDYDDKAMDKADHFANLIPEIATQTSSLERRAIDAERIVEAMKKAKYME
                     EYVGEEFEGVVASVVKFGMFVELPNTIEGLIHVTTLPEYYHFNERTLTLQGEKSGKVF
                     RVGQQIKVKLIRSDKETGDIDFDYLPSDFDIVEKVSKSSREGRPNRSSKREHQHRISD
                     RDNKNKNTSKKKASRKPKRNSDSKSHHHKDDRTTGSTKKKTKKPFYKGVAKKGQKRKS
                     KKG"
     gene            4429..4896
                     /gene="smpB"
                     /locus_tag="VBE34_001647"
     CDS             4429..4896
                     /gene="smpB"
                     /locus_tag="VBE34_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEO7264348.1"
                     /translation="MVKGQGNVVAQNKKAHHDYTIVETIEAGIVLTGTEIKSVRAARI
                     TLKDGYAQIKNGEAWLINVHITPYDQGNIWNQDPDRTRKLLLKKREIEKISNELKGTG
                     MTLVPLKVYLKDGFAKVLLGLAKGKHDYDKRESIKRREQNRDIARQLKNYNSR"
     gene            complement(4980..6368)
                     /locus_tag="VBE34_001648"
     CDS             complement(4980..6368)
                     /locus_tag="VBE34_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO7264349.1"
                     /translation="MSKNNNDHTQKSENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SISLTGPSIVLVYAITGAFMFFMMRAIGEMLYQDPNQHTFINFITKYLGPGWGFFSTL
                     SYWISLIFIGMAEITAVGEYVQFWFPEWPSWIIQIVFLAILSSINLIAVKAFGETEFW
                     FAMIKVIAILGLIATGIFMVLTNFDTGHGYHASISNITNHFEWFPKGKLNFFMAFQMV
                     FFAYLAIEFVGVTTSETANPRKVLPKAIQEIPMRIILFYAGSLLAIMAIFPWQQLPVN
                     ESPFVTVFKLAGIKWAAALINFVVLTSAASALNSTLYSTGRHLFQLANESPNALTKAL
                     KLDQLSRQSVPSRAIIASAVIVGASALISVLPGISDAFSLITASSSGVYISIYVLIMI
                     AHWKYRKSPDFMEDGYKMPAYKILSPITLLFFLFVFVSLFLQDSTYIGAIGATIWIIG
                     FGLYSHFKHKSH"
     gene            complement(6497..7423)
                     /locus_tag="VBE34_001649"
     CDS             complement(6497..7423)
                     /locus_tag="VBE34_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7264350.1"
                     /translation="MALLSVIVPCYNEQETVSTFLTEIKKVESEMARYTHFEYIFVND
                     GSTDRTLELLKKAAKQFDNVHYLSFSRHFGKDAALLAGLEHTTGDFITVMDVDLQDPP
                     TLLPEMYLKLQEGYDIVATRRKDRKGEPLIRSLFAKLFYKLINQVSDTKMVDGARDFR
                     LMTKQVVDSILELNEVNRFSKGIFSWIGYDVAYISYENRERIAGKTSWSFFNLLKYSL
                     DGFINFSEIPLAIATWIGTLSSVLSLLAIIFIIIRKLLFGDPVSGWASTVTIVLFMGG
                     IQLLSLGIIGKYISKIFLETKKRPVYIVKEEG"
     gene            complement(7552..7944)
                     /gene="gloA"
                     /locus_tag="VBE34_001650"
     CDS             complement(7552..7944)
                     /gene="gloA"
                     /locus_tag="VBE34_001650"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEO7264351.1"
                     /translation="MPFLHTCIRVKDLDASIAFYQEALGFKEVRRNDFPENQFTLVYM
                     SLEDDPSYELELTYNYDHEAYDLGNGYGHIAVGVDDLETTYDAHQKAGYSVTKISGLP
                     GKPNMFYFIQDPDGYKIEVIRLSQFKAD"
     gene            complement(8032..8706)
                     /locus_tag="VBE34_001651"
     CDS             complement(8032..8706)
                     /locus_tag="VBE34_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7264352.1"
                     /translation="MTKEDIKKQVRRAFDFRMAIRVYNNNDIPKEDMEYILDTAWLSP
                     SSVGLEGWRFLVLDRQTIAKFRDKLKEVAWGAQYQLDTASHFVLLLAEKGAYYNADSM
                     INSLIRRGLGDPAALESRIPLYKSFQENDMKIDSERSLWDWTAKQTYIALGNMMTAAA
                     MIGVDSCPIEGFDYEKVNNILSKEGLIDDKKEAISCMVSFGYRLREPKHSRARKERQE
                     VITWVE"
     gene            8973..9815
                     /locus_tag="VBE34_001652"
     CDS             8973..9815
                     /locus_tag="VBE34_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO7264353.1"
                     /translation="METYTLSNTLNIPKIGFGTWQLTEGEEAYKAVTHALKVGYTHID
                     TAQIYGNEHSVGRAIRDSGLARESIFLTTKIWNDKHDYHLAKASIDESLQKLGVDYID
                     LLLIHWPNPKALRENDAWKAGNAGTWKAMEEAYKEGKVKAIGVSNFMKHHLEALFETA
                     EIKPMVNQIILAPGCAQEDLVRFCKGNDILLEAYSPFGTGTIFENESIKAIAEKYGKS
                     VAQVALRWSLDNGFLPLPKSATPKNIEANLDIFDFQLNEDDIATLIQLDSGIKPKDPD
                     NVSF"
     gene            10006..10728
                     /locus_tag="VBE34_001653"
     CDS             10006..10728
                     /locus_tag="VBE34_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO7264354.1"
                     /translation="MRLDKFLVECGLGSRTQVKLILKKKQISVNSNSETSPKVQVDEY
                     RDEIKYNGTLVSYEKFVYYMLHKPKGVISATDDPSHKTVLDLLDKTARDKAVFPVGRL
                     DIDTTGLLLITNNGELAHKMLSPKKHVDKCYEAKISGIMTEDDILAFDKGIILKDFTC
                     LPALLEIVEVNQVKKQSLVKITIKEGKFHQVKRMVAACGKEVLELKRLSMGNLQLDKQ
                     LESGQWRRLTIKEIEKLEKHMQ"
BASE COUNT         3671 a         1706 c         2077 g         3282 t
ORIGIN      
        1 aaatctataa cgatgccaat attattagta agcctaggaa ttatagttgt tattttaatt
       61 attggctatt tagcagttgt agtatttgct ccaaaaactt tccgtttaaa aagttaattt
      121 taattgtggt ataataacag aggcatgtga taacataggt ttagttacat aaaggaaatt
      181 ataatggaaa taacatctca aatagatgtt aaatctcaat aaaactgcta agatagttag
      241 tggtagaaaa gtttagaaag aagatgatgt tttatctcag ttgaaacggt taactggaag
      301 caaaatttaa aagtggcttg gctcggtaat ttttttactg gggcaagctt ttcacttgtt
      361 atgcctttta tggtattata tgtagaacaa ttaggagcac ctagcaacaa agtagagtgg
      421 tatgcaggcc tatcagtatc cttatcagcc ttgtcttcag ctttagttgc cccattatgg
      481 gggcgcttag ctgataaata tggtagaaaa ccgatgatgg taagagcagg gttgatgatg
      541 acttttacta tgggaggcct tgctttcatt catagtgtta caggacttct gattttacgt
      601 atattaaacg gcatttttgc aggctatgta cctaactcta cagctttgat agcaagtcaa
      661 gctcctcaag aggaatcagg atatgcctta ggaaccttag cgacaggagt gactggaggt
      721 atgttaattg gtccactttt agggggactt ttagcagagt ggtttggtat tagggaagtt
      781 tttttattag tgggaaccat attgttaata tctactttaa tgaccatttt tatggtaaag
      841 gaagatttca aacccatttc taacgaagaa accatgccaa ctacagaagt gttcaaaagc
      901 gttaaaagct tacaaatttt aatagggctg tttgtaacaa gcatgattat tcaaatttct
      961 gcacaatcaa ttgcacctat tttaacactt tacattcgtc atttaggaca gactgaaaac
     1021 ttgatgtttg tttccggatt aattgtttct ggtatgggat tctctagtat tctgagtagt
     1081 ccaaagctag gaagaatcgg ggatcgcata ggcaatcatc gtttgctatt attagctcta
     1141 ctgtatagtt tcctcatgta tgtcttatgt tcattggcac agacttcatt acagctaggg
     1201 gttattagat tcctgtatgg ttttggaaca ggtgccttga tgccgagtat taattcaatc
     1261 ttgactaaaa ttgctccaag acaaggttta tcaaggattt tcagttataa ccaaatgttt
     1321 agcaatttag gacaagtatt aggtcctttt gtcggttcag cagtttctat tcatttagga
     1381 ttccgatggg ttttctttgt gactagcttc atcgttttag ctaattttgt ttggtgtttt
     1441 attaatttta gaaaatatat tagagttaag gaaattgtgt gagagttaaa attaatttaa
     1501 agtgtagctc ttgtggtagt aagaattatt taaccagtaa gaataaaagt aatcatcccg
     1561 ataaaatcga ggttccaaag tattgcccaa aggagagaaa agtaacacta catattgaat
     1621 cttaataagt tttatgttag aatgtacttt gaccatagtg gaggaattaa catgtataac
     1681 ctattactca ccattctact tgttttatca gtattattga ttatctcaat tttcatgcaa
     1741 ccacaaaaga atccaagtag taatgttttt gatagtagcg gttctgaagc cctttttgaa
     1801 cgtagtaaag ctcgaggatt tgaagctttt atgcaacgtt ttacaggtgt tttagtcttt
     1861 ttctggctac taatcggctt agtattatct attttatcaa gtcattaatg atagctaaat
     1921 acagataagt gaggatgggg gatagcccaa cctcactttt tgttagtaga atggttgtag
     1981 agtaataata aagtgactaa agttactaga aaaggaaata atgaaagaaa aaattataaa
     2041 atatttggaa gaacacggta aaaccagcgt taatgattta gctgctgccc tagatatggc
     2101 aggcgcaaaa gcatttccaa aattaatcaa aacaatctca aatttggaga gccacaggca
     2161 attaagattt gacgataatg gaagtctttc tcttcaaaag aaagaagcta agaaaaagga
     2221 aatcacagta aggggtcttt ttagagcaaa taaagcagga tttggttttc ttagtattga
     2281 ccaagacgaa gatgatatgt ttatcggaaa aaacgatatt gcttatgcta ttgatggtga
     2341 cacggtagaa gctgttgtaa agaaaccagc ggaccgtctt aacgggactg cagcagaagc
     2401 gcgtgttgtt aatattgttg aacgtagttt aaagacgctt gttggtaagt ttgtcttaga
     2461 cgatgagcgc cctaaatacg caggatatat caaatcgaaa aatcaaaaaa tcaatcaaaa
     2521 gatttatata cgcaaagaac cggtcgtttt agacggaaca gaaattatca aagtagatat
     2581 tgataaatat ccgacacgag gacatgatta cttcgtagca agtgttcgtg atatcgttgg
     2641 acatcaagga gatgttggta ttgatgtttt agaagtcttg gaaagtatgg atatcgtctc
     2701 agaatttcct gaggacgtta ttgcagaagc caatgctatc ccagatgctc caacggagaa
     2761 agatttaatt gggcgtgttg atttacgtca agaggtcact ttcactattg atggagcgga
     2821 cgccaaagat ttagacgatg ctgtgcatat taaattactg gataatggtc attttgaact
     2881 tggagttcat atcgcagatg tatcatacta cgttactgaa ggctctgcct taaatcgtga
     2941 agcacttagt cgtggtacgt ccgtatatgt cacagatcgc gttgtaccaa tgttaccaga
     3001 gcgtctatct aacggtatat gttcattaaa tccaaatctt gatcgtttaa cgcagtcttg
     3061 tattatggaa attgaccaaa atgggagagt tgttaatcat caaattacac agtcagtgat
     3121 taataccact tatcgtatga cttatatcgc tgtaaatgat attattgctg gcgatgagga
     3181 aatctgctca gaatatgagt ctattgtgtc ctcagtacag catatggtca ctttacacca
     3241 tactttggaa gctatgcgta cgcgtcgtgg agcgctgaat tttgacacat ctgaagcaaa
     3301 aattatggtt aatgataagg gaatgccagt tgatattgtt attcgcaaca gaggcattgc
     3361 tgaacgcatg attgagtcat ttatgttggc ggcaaatgaa acggttgctg aacattatgc
     3421 tcgtctaaaa ctgccattta tttatcgtat acacgaagag cctaaagctg aaaaattaca
     3481 aaaattcatc gattatgctt ctgttttcgg tgttcagatt caaggaacag caactaaaat
     3541 cacccaatct gctttacagg actttatgaa aaaagtacaa gggcagccag gaagtgaagt
     3601 gttatctatg atgttattgc gctcgatgca acaagctaga tattctgaac acaaccatgg
     3661 acattatgga cttgctgcgg aatattacac tcactttact agccccattc gacgttatcc
     3721 agacttgtta gttcatcgta tgattcgtga ttatgatgat aaagcgatgg ataaagctga
     3781 tcattttgct aaccttatcc cagaaattgc aactcaaacg tctagtcttg agagacgtgc
     3841 cattgatgct gaacgtattg tagaggcaat gaagaaagcc aaatatatgg aagaatatgt
     3901 tggtgaggaa tttgaaggtg tggttgcaag cgttgttaaa tttggtatgt ttgtagaatt
     3961 accaaatact attgaaggat tgattcacgt aacgaccttg cctgaatatt atcactttaa
     4021 tgaacgtacg ctgacgcttc aaggtgagaa atctgggaaa gttttccgtg ttggtcaaca
     4081 aattaaggtt aagttgattc gttctgataa ggaaactggt gatatcgatt ttgattattt
     4141 accatcagat tttgatattg ttgaaaaggt aagtaaaagc tcgcgtgagg gtcgtcctaa
     4201 tagaagttct aaacgagaac atcagcatag aatttctgat cgtgataata agaataaaaa
     4261 tacatctaag aaaaaagcat ctcgaaaacc caaaaggaac tcggatagca aatcacatca
     4321 tcataaagac gatagaacaa caggttcaac caagaaaaaa acgaaaaaac ctttctataa
     4381 aggcgttgct aagaaaggtc agaaacggaa atctaagaaa ggttaagtat ggttaaaggt
     4441 caaggaaacg ttgtagcaca aaataaaaaa gcgcatcacg actatactat cgttgaaaca
     4501 atagaagcag gaattgtttt gacaggaaca gaaatcaaaa gtgtccgcgc agcgcgtatt
     4561 acattgaaag atggatatgc acaaataaaa aatggtgaag cgtggttgat taatgttcat
     4621 attacaccat atgatcaagg taacatatgg aatcaagatc ctgatcgtac acgaaagtta
     4681 ttactaaaaa aacgtgagat tgaaaaaata tcaaatgaac tgaaagggac tggtatgact
     4741 ctggtacctc taaaagttta cttaaaagat ggttttgcta aagttctatt gggtttggca
     4801 aaaggaaaac atgattatga taagcgagag tcaatcaaac gtcgtgagca aaaccgtgat
     4861 attgcccgtc agttaaaaaa ctataattca cgatgattat caaaaggtta gggaattgcc
     4921 taaccttttt tggacgaaaa aagtgaagtt gattgaccaa cttcactaat aatatagact
     4981 taatgtgatt tatgtttgaa gtgactatat aaaccaaatc caataatcca gatggttgct
     5041 ccaatagccc cgatgtatgt tgaatcttgc aagaagagag aaacgaatac aaagaggaag
     5101 aagagaagtg tgattggtga aaggattttg taggcaggca ttttataacc atcttccata
     5161 aaatcaggtg atttacgata tttccaatgt gcaatcataa taagaacata gatagaaatg
     5221 taaacaccag aagatgatgc agtaattaag gagaaggcat ctgaaatacc aggtaaaaca
     5281 ctgataagtg ctgaagctcc aacgattaca gctgatgcaa taatagctcg acttggaacg
     5341 ctttgacgag ataattggtc taattttaac gcttttgtaa gagcatttgg tgattcatta
     5401 gccaattgga ataaatgacg ccctgttgag tacaaagtag aattaagagc agatgctgca
     5461 gacgttagga caacaaagtt aatcaaagcg gcagcccact taatcccagc tagtttgaat
     5521 actgtaacga aaggtgactc attaactggt aactgttgcc atgggaagat ggccataatt
     5581 gctaataatg aacctgcgta gaataaaata atacgcatcg gaatttcttg gattgctttt
     5641 ggtaaaactt tacgtggatt agctgtttca gaagttgtta ctccgacaaa ttcaatagct
     5701 aaataagcaa agaataccat ttggaaagcc atgaagaaat tcaatttacc tttagggaac
     5761 cattcaaaat gattggtgat atttgagatt gaagcatggt atccgtgacc ggtgtcaaag
     5821 tttgtcaata ccataaagat accggttgct attaaaccaa gaattgcaat aacttttatc
     5881 atagcaaacc aaaactcggt ttctccgaat gcttttacag caattaagtt gatagaactt
     5941 aggatagcta aaaaaacaat ttggataatc caggatggcc attcagggaa ccaaaattga
     6001 acatactctc ctacggctgt aatctctgcc ataccgataa atattaggga aatccaataa
     6061 gaaagtgttg agaaaaatcc ccaaccgggt cctaaatatt tggtaatgaa attaataaaa
     6121 gtatgctgat ttggatcttg ataaagcatc tctccaatag cacgcatcat gaagaacatg
     6181 aaagcgccag tgatagcata gacaagaact attgaaggac ccgtcaggct aattgaacga
     6241 cctgcaccaa gaaaaagccc agttccgatt gtccctgcaa tcgcaattaa ctgtacgtga
     6301 cgattttcta ggccgcgtac cataccattc tcagattttt gagtgtggtc attattattt
     6361 tttgacatta taaatactcc atctattttt gaaaaactcc ttcattatat aatttttttg
     6421 caaaattcgc aataattact atacaatatt tttctatgat aaaatctcct agctaagtga
     6481 gctaagagat ttcaattcat ccctcttcct tgacaatata gactgggcgt tttttagttt
     6541 ctaggaaaat cttagagatg tatttgccta taattcctag cgacaataac tgaataccac
     6601 ccataaaaag tacaatggta actgtgcttg cccaacctga aacgggatcc ccaaatagta
     6661 attttcgaat tataataaaa atgatagcta aaagtgatag tactgaacta agggttccaa
     6721 tccaggtagc tatagctaat ggaatttctg agaagttgat gaaaccgtct agagagtatt
     6781 ttaagagatt aaagaaactc catgaggtct tacccgcaat tcgttctcga ttctcataac
     6841 taatataagc gacatcatat ccaatccagg aaaaaatccc ttttgagaag cgattcactt
     6901 catttagctc gagaatactg tcaacaactt gttttgtcat gagacgaaaa tcacgcgcac
     6961 catcaaccat tttagtatct gacacttgat tgattaattt ataaaaaagc ttggcaaaaa
     7021 gagagcgtat caacggctcc cctttacgat cttttcgcct tgtagcgaca atatcataac
     7081 cttcttgaag ttttaggtac atttctggta ataaagtggg tggatcttgc aagtcaacat
     7141 ccataacagt gataaaatca ccagtagtat gttctaaacc agccaataaa gcagcatctt
     7201 taccaaaatg gcgtgaaaat gaaaggtaat gaacattgtc aaattgttta gctgctttct
     7261 taagaagttc gagtgtacga tcggtagaac catcgtttac gaaaatatat tcaaaatggg
     7321 tgtaacgagc catttcggat tcaacttttt tgatttctgt taaaaaagtt gagacagtct
     7381 cttgttcatt ataacaaggg acaataactg ataataaagc catttgtaat tctccgtatc
     7441 attcttgctt aaattttaac ataatatggg aaaaaataga aagattataa ggtaatcata
     7501 aaaaggacta ggtttttgcc tcagtcctta acttaatatg atgagatgtt attagtcagc
     7561 tttaaattga ctgagtcgaa taacttcaat tttatagcca tctggatctt gaataaagta
     7621 gaacatatta ggtttacctg gtaatccaga aatttttgtt acagagtaac ctgctttctg
     7681 atgggcatca taagtagttt ccaaatcgtc tacaccaaca gcaatgtgac catacccatt
     7741 tcctaaatca taagcttcgt gatcatagtt ataggttaac tctaattcgt aactagggtc
     7801 gtcctctagg gacatataga ctaaagtaaa ttgattttca ggaaagtcat tacgacgaac
     7861 ttccttaaaa ccaagtgctt cttgataaaa agcaattgag gcgtccaaat ctttaacgcg
     7921 aatacaagta tgtaaaaatg gcatagtctc tctcctattt ctttattaaa cgtattatgt
     7981 caaaataaaa ttatcttttc aaaaatattg attataaatt aggctgtcta tttactcaac
     8041 ccaagtaatg acctcttgac gttctttcct agcacgggaa tgtttaggtt cacgcaaacg
     8101 ataaccaaat gaaaccatac aagaaatagc ttctttctta tcgtctatta acccttcttt
     8161 actaagaata ttattgacct tttcatagtc aaatccttca attgggcatg aatctacgcc
     8221 gatcatagca gcagccgtca tcatattacc aagtgcgata taagtttgtt ttgctgtcca
     8281 atcccaaaga gaacgttcag aatcaatttt catatcgttt tcttggaagg atttgtagag
     8341 aggtatccta ctctcaaggg cggctggatc acctagacca cgtcgaatga gactgttaat
     8401 catgctatca gcgttataat aagcaccttt ttcagctagt agtaaaacaa agtgactagc
     8461 agtatctagt tgatattgtg ctccccaagc aacttcctta agtttatctc gaaattttgc
     8521 aattgtttgt ctatctagta ctaaaaatcg ccagccttct aaaccgactg aagaaggact
     8581 caaccaagca gtgtctaaaa tatactccat atcttcttta ggaatatcat tattattata
     8641 aacacgaatc gccatacgaa aatcaaaagc tcttcgaact tgttttttga tatcttcttt
     8701 tgtcatttta gatcctcctg ttattaatgc tatcatatca caatttagaa aaaatctcag
     8761 caggcggtat cgtggcaaag tagagctaca aaattctaat gactgtcaga aaaataaagg
     8821 gaatgaacaa aatgataggg ttttgatgaa gaaagttata taattttaca taaggagggg
     8881 ataataccat atatcaaact atcatttaga tgtttaaaat agatgttttt cactatgaaa
     8941 ttcatttttg tcacatagaa aaggagattg ttatggaaac atatactcta tcgaatactc
     9001 ttaacattcc taaaattggt tttgggacat ggcaactaac tgaaggtgaa gaagcttata
     9061 aagcagtgac tcatgcttta aaagttggtt atactcatat tgatactgct cagatttatg
     9121 gtaatgaaca tagtgttgga cgtgctataa gagacagtgg tttggcgcga gaaagtatct
     9181 ttttgacaac taagatttgg aatgacaaac atgactatca tttagccaaa gcttctattg
     9241 atgaatctct gcagaaatta ggtgttgatt atattgatct tcttttgatt cattggccaa
     9301 atccaaaagc tcttcgtgaa aatgatgcgt ggaaagctgg gaatgcaggc acttggaaag
     9361 cgatggaaga agcttataaa gaaggaaagg taaaagctat tggtgtttcg aatttcatga
     9421 aacatcactt agaggcttta tttgaaaccg ccgaaataaa acctatggtt aatcaaatta
     9481 tcctagcacc aggttgtgct caagaagatt tggttcgctt ctgtaaagga aatgatatct
     9541 tattagaagc ttacagtcca tttggaacag gtactatttt tgaaaatgaa tctataaaag
     9601 ctattgctga aaaatatggt aaatcagttg cacaagtagc tcttcgctgg tcacttgata
     9661 atggcttttt accattacct aagtcagcga ctcccaaaaa tattgaagca aaccttgata
     9721 tttttgattt tcaattaaac gaagatgata ttgctacctt aatacaacta gattcaggta
     9781 ttaaacccaa agatccagat aatgtctcat tttaataaga aaagatggaa atgtatttcc
     9841 atctttttta tgtgggatag ttccttaaag ttaagaaaaa cattaagacc tccattgacg
     9901 aggagaatat attaatgata gtattactag aaaagtagta ataaaggatg agtatggtaa
     9961 aatcctttac aatatatcat tatcgaaaga ggttataaca tctaaatgag acttgataaa
    10021 tttttagtag aatgcggact tggttcacgt actcaagtca aattaatatt gaaaaaaaag
    10081 cagattagtg ttaatagtaa tagcgagaca tctccaaaag tacaggtaga tgaatataga
    10141 gatgagatta aatataatgg aactctagtg tcatatgaaa agtttgttta ttatatgctt
    10201 cataaaccca aaggtgttat atcagcaacg gatgatcctt cgcataaaac cgtattagat
    10261 ttgctagaca aaactgctcg agataaagct gtttttccag tggggcgttt agatattgat
    10321 acaacaggcc ttttgttaat aactaataat ggggaattag cgcataaaat gttatcacct
    10381 aaaaaacacg ttgataaatg ctatgaggca aaaatctcag ggataatgac agaagacgat
    10441 attttggcat ttgataaagg gattattctg aaagatttta cttgtttacc agcattatta
    10501 gaaattgtgg aagtaaatca ggtgaagaag cagagtcttg ttaaaatcac tattaaggaa
    10561 ggaaaattcc atcaagttaa acgtatggtt gctgcgtgtg gcaaggaagt cttagagcta
    10621 aagcgtctaa gtatgggaaa tctacaatta gataaacagc tagaaagtgg acaatggaga
    10681 cggctaacaa taaaagaaat agaaaaatta gaaaaacata tgcaataata taaaat
//
LOCUS       DATRUK010000064        10542 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.064, whole genome shotgun sequence.
ACCESSION   DATRUK010000064 DATRUK010000000
VERSION     DATRUK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10542
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.064"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(27..1499)
                     /locus_tag="VBE34_001654"
     CDS             complement(27..1499)
                     /locus_tag="VBE34_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7264355.1"
                     /translation="MEKTKSFFGHPRGLSTLFFTEMWERFSYYGMRAILLYYMYYSVS
                     QGGLGMDKTVAASIMAVYGSLVYLSSVIGGFVSDRILGSRKTVLYGGILIMLGHIALA
                     TPFGQTALFISIALIILGTGLLKPNVSEMVGNLYGENDSRRDAGFSIFVFGINLGAFI
                     SPIVVGYLGQEVNFHLGFSLAAIGMFFGLLQYTLDGKKYLTEESLRPNDPLSPEEKSS
                     LYKKVGLILIGIVIVLILLHLMHMLTIEVIIDIFSIIAIAIPIIYFIKILSSKKISSV
                     ERSRVWAYIPLFIASILFWSIEEQGSVVLALFADEQTKLYLNFFGHHINFPSSYFQSM
                     NPLFIMLYVPFFAWLWAKWGSKQPSSPKKFAYGLFFAGASFLWMMLPGLLFGVNAKVS
                     PLWLTMSWAIVIVGEMLISPVGLSATSKLAPKAFQAQMMSIWFLSNAAAQAINAQIVK
                     LYTPDTQILYYGVVGGITVVFGFILLFYVPRIEKLMSGVK"
     gene            complement(1730..2566)
                     /locus_tag="VBE34_001655"
     CDS             complement(1730..2566)
                     /locus_tag="VBE34_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO7264356.1"
                     /translation="MNKKKRLNLTFVYILLIVLSIMWLFPIVWVVLTSFRGEGSAFVN
                     YFIPKTWTLDNYAKLFTQNTFPFGQWFLNTLFVATCTCILSTLITVAMAYSLSRIKFK
                     HRNGFLKLALVLNMFPGFMSMIAVYYILKALNLDQTLTALIFVYSAGAALTFYIAKGF
                     FDTIPYSLDESAMIDGATRLDIFLKITLPLSKPIIVYTALIAFMGPWMDFIFAKVILG
                     DATSKYTVAIGLFSMLQQDTINQWFMSFTAGSVIIAIPITILFMFMQKYYVEGITGGS
                     VK"
     gene            complement(2566..3936)
                     /locus_tag="VBE34_001656"
     CDS             complement(2566..3936)
                     /locus_tag="VBE34_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO7264357.1"
                     /translation="MNKKLISLDGMSLKEVFQKGDLATKLSFLIMGLANLKNKQIVKG
                     LLFLISEILFLITFVYQVIPAVKGLISLGTQEQGMTTKTVDGIKIQVATQGDNSMLML
                     IFGLASLIFCCVFAYIYWSNIKSAAHLLTLKEEGREIPSFKKDIKSLTDGRFHMTLMS
                     IPLIGVLLFTILPLVYMICLAFTNYDHNHLPPKSLFDWVGFANFGNIFSGRMASTFFP
                     VLSWTLIWAVFATVTNFFFGIILALLINTKGLKFKKMWRTIFVITMAVPQFISLLIMR
                     NLLSDAGPVNALLIKWGLISSAHPLPFLSDPVWAKFSIIFVNMWVGIPVTMLVATGII
                     MNLPAEQIEAAEIDGANKFQVFQSITFPQILLIMTPTLIQQFIGNINNFNVIYLLTQG
                     GPTNSTYYQAGSTDLLVTWLYNLTVTAADYNLASVVGILIFILSAVFSLLAYTRTNSY
                     KEGAAK"
     gene            complement(4034..5281)
                     /locus_tag="VBE34_001657"
     CDS             complement(4034..5281)
                     /locus_tag="VBE34_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO7264358.1"
                     /translation="MEKNTWKKLLVSTAALSVVAGGAIAATHSNSVDAASKTTIKLWV
                     PTDSKASYKAIVKKFEKENKGVTVKMIESNDSKAQENVKKDPSKAADVFSLPHDQLGQ
                     LVESGVIQEIPEQYSKEIAKNDTKQSLTGAQYKGKTYAFPFGIESQVLYYNKTKLTAD
                     DVKSYETITSKGKFGQQLKAANSYVTGPLFLSVGDTLFGKSGEDAKGTNWGNEAGVSV
                     LKWIADQKKNDGFVNLTAENTMSKFGDGSVHAFESGPWDYDAAKKAVGEDKIGVAVYP
                     TMKIGDKEVQQKAFLGVKLYAVNQAPAGSNTKRISASYKLAAYLTNAESQKIQFEKRH
                     IVPANSSIQSSDSVQKDELAKAVIEMGSSDKYTTVMPKLSQMSTFWTESAAILSDTYS
                     GKIKSSDYLKRLKQFDKDIAKTK"
     gene            5537..6565
                     /locus_tag="VBE34_001658"
     CDS             5537..6565
                     /locus_tag="VBE34_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7264359.1"
                     /translation="MVTIKDVAAKAGVNPSTVSRVLKDNESISSKTKERVKKAMEELG
                     YVPNVAAQMLASGLTQNIGVIFPPLVLPDRLSEPFFMEILSTLTNEAKEHGFTVSIAT
                     SMSSEELLEQVKLMHLQKRVDGFIVLYSDQKDPIREYLLKYKLPFVVVGAANGYENEI
                     TYIDNDNQLMGKSAIDYLYKKGHKSIQFVTDDLNSEVSEERYLGYFKGARKLGLNQKP
                     ALLFDRGNPQVLEEFINRVKEEETTALIVIGDTVSVRVMQFLSFYKLKVPDDISIMTF
                     NNSLFSHLIHPYLSTFDINVNNLGRTSVRRLIDIIKSPDKVFSETIIVPFTLEERESV
                     RDISKNNN"
     gene            6686..8182
                     /gene="malQ"
                     /locus_tag="VBE34_001659"
     CDS             6686..8182
                     /gene="malQ"
                     /locus_tag="VBE34_001659"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEO7264360.1"
                     /translation="MKKRASGVLMHITSLPGDLGIGTFGREAYAFVDFLVETDQKFWQ
                     ILPLTTTSFGDSPYQSFSAVAGNTHLIDFDLLTLEGFISKDDYQNISFGQDPEVVDYA
                     GLFEKRRPVLEKAVKNFLQEERATRMLSDFLQEEKWVTDFAEFMAIKEHFGNKALQEW
                     DDKAIIRREEEALAGYRQKLSEVIKYHEVTQYFFYKQWFELKEYANDKGIQIIGDMPI
                     YVSADSVEVWTMPELFKLDCDKQPLAIAGVPADDFSDDGQLWGNPIYNWDYHKESDFD
                     WWIYRIQSGVKMYDYLRIDHFKGFSDYWEIRGDYQTANDGSWQPAPGPELFATIKEKL
                     GDLPIIAENLGYIDERAERLLAGTGFPGMKIMEFGFYDTTGNSIDIPHNYTENTIAYA
                     GTHDNEVINGWFENLTVEQKAYAENYMRRLPNEPITETVLRTLYATVSQTTITCMQDL
                     LDKPADSRMNMPNTVGGNWQWRMRKEDLTENRKAFLKEITTIYNRGNK"
     gene            8194..10458
                     /gene="glgP"
                     /locus_tag="VBE34_001660"
     CDS             8194..10458
                     /gene="glgP"
                     /locus_tag="VBE34_001660"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEO7264361.1"
                     /translation="MTRNFTTYVGQQGKVLSELTNEEIYVELLNFVKEEAAAKSKNSS
                     QRKVYYISAEFLIGKLLSNNLINLGIYKDVKKELELVGKSIAEIEDVEPEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFRNNQQEAEANYWIENNSWLVPTDI
                     SYDVPFRDFTLKSRLDRIDVLGYKKDTKNYLNLFDIDGLDYNLIEKGITFDKTEIKKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLLIDEAIERGSNLHDLAEYAYVQINDT
                     HPSMVIPELIRLLTEKHGFEFDEAVSVVRNMVGYTNHTILAEALEKWPLEYLNEVVPH
                     LVTIIKKLDQMIREEQTNPEVQIIDEAGRVHMAHMDIHFSTSVNGVAALHTEILKNSE
                     LKVFYDIYPDKFNNKTNGITFRRWLEFANQDLADYLKELIGDSYLTDATQLEKLLTYA
                     DSNEVHDKLAAIKFKNKLALKRYLKENKGIELDEYSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKRGHFPSRKLTVIFGGKAAPAYTIAQDIIHLILCLSELINNDPEVNKYLNV
                     HLVENYNVTVAEKLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGKENIYTFGKDSDTIINLYETSGYRSKDYYDKDKVIREAVDFIISDDIVSLGNAE
                     RLKRLHDELVGKDWFMTLIDLKEYIAVKEQVLADYEDYESWNKKVIHNIAKAGFFSSD
                     RTIEQYNQDIWHSN"
BASE COUNT         3598 a         1694 c         1973 g         3277 t
ORIGIN      
        1 tgtaacctag ttctttttag atgtctttat ttaactccag acattagttt ttcaatgcgt
       61 ggaacataaa ataagaggat aaatccaaat acaactgtta taccaccaac aacaccataa
      121 taaagaattt gagtatcagg tgtgtacaat ttaacgattt gagcgttaat agcttgtgct
      181 gcagcattac ttaagaacca gatgctcatc atttgagctt ggaatgcttt aggtgcgagc
      241 ttactagttg ctgataatcc aactggtgag attagcattt ccccaacgat gacaatagcc
      301 caactcattg ttaaccaaag agggcttact ttagcgttaa ctccaaagag taaacctggt
      361 agcatcatcc ataagaatga agctccagca aagaaaagtc catacgcaaa ttttttaggt
      421 gatgaaggtt gcttacttcc ccatttagcc cataaccaag caaagaatgg tacataaagc
      481 ataatgaaga gagggttcat actttggaaa taacttgatg ggaaattaat atgatgccca
      541 aagaagttaa ggtaaagttt tgtttgttca tctgcaaata aggctaagac aactgaacct
      601 tgttcttcaa ttgaccaaaa tagaattgag gcgataaaga gagggatata tgcccacact
      661 cgagaacgct caacagaaga aatcttttta ctacttaaaa tcttgataaa ataaataatt
      721 gggatggcga ttgcaataat actaaaaata tcgataatta cttcaattgt tagcatatgc
      781 atcaagtgaa gtagaataag tacaataaca ataccaataa ggataagccc aactttttta
      841 tatagagagg acttttcttc aggacttaaa ggatcgtttg gtctgagact ctcttcagtc
      901 aaatattttt ttccatctaa ggtatattgg aggagaccaa agaacatacc aatagcagca
      961 agtgagaaac caagatggaa atttacttct tgtccgaggt accccactac aatgggtgaa
     1021 ataaaagcac caaggttaat accaaaaaca aagatactaa aaccagcatc acgacgagaa
     1081 tcattttctc catataaatt accaaccatc tctgatacat ttggttttaa tagtccagta
     1141 ccaaggataa ttagagcaat cgaaatgaat agagctgttt gaccaaatgg tgtagctaga
     1201 gcaatatgac ctagcatgat tagaatacca ccatacagaa cagttttacg actacctaga
     1261 atacggtcgc tgacaaaacc accaattact gatgagagat aaaccagtga accatagaca
     1321 gccatgattg atgcagcgac agtcttgtcc ataccaaggc caccttgaga aacactatag
     1381 tacatatagt acagcaaaat agcacgcata ccatagtatg agaatctttc ccacatttca
     1441 gtaaagaaaa gagtggacaa accacgagga tgtccaaaga atgattttgt tttttccatg
     1501 aaaaacgctc cttttgtaga taacactatt aataatatag taaatagaag caactgtcaa
     1561 attacttagc actatcaagt tatttttttg tagaggttaa cacccttggt aaaccaagtt
     1621 ttttaactat ctagcttaga aaaaggcttg aattcaatag aacccaagcc ttttttagaa
     1681 aagaaaccac agtgaaataa aaatgtcgtg tctttaaatt atctgaagat tattttacag
     1741 atccacctgt aataccttca acatagtatt tttgcatgaa catgaaaagt attgtgattg
     1801 gaattgcaat aataacagat cctgctgtaa atgacatgaa ccactggttg atggtatctt
     1861 gttgcaacat tgaaaagaga ccaatagcga ctgtgtactt gctcgtagca tcacctaaaa
     1921 taacttttgc aaagataaag tccatccaag ggcccataaa agcgattaat gcagtataaa
     1981 cgataatggg ttttgacaat ggtagggtga ttttaaggaa aatatccaaa cgggtcgccc
     2041 catcaatcat agcagactca tccaaagagt atggaatagt atcaaagaaa ccttttgcga
     2101 tgtagaaggt taatgctgct cctgcagaat agacaaatat taaagcggtt aatgtttggt
     2161 ctagatttag agcttttagg atgtagtaaa ctgcaatcat tgacatgaac ccaggaaaca
     2221 tattcaagac taaagctagc tttaggaaac cattacggtg tttgaatttg attctgctga
     2281 gagaataagc cattgctaca gtgattaaag ttgatagtat acaagtacat gtagcgacaa
     2341 ataatgtatt taagaaccat tgaccaaatg ggaatgtgtt ttgggtgaag agtttagcgt
     2401 agttgtctaa tgtccaagtt ttaggaataa agtagttgac aaaggcactt ccttcaccac
     2461 ggaagcttgt gagaacgacc caaacaatgg gaaagagcca cataattgat agtactatca
     2521 ataaaatata aacaaaagta aggttaagac gttttttctt attcattatt tagctgcccc
     2581 ttccttgtaa gaatttgttc ttgtgtaagc tagtaaacta aatactgctg aaagaataaa
     2641 gattaagata ccaacaacag atgctaagtt ataatctgca gcagttactg tcaaattgta
     2701 tagccaagtg acaagtaaat cggtgctacc tgcttggtag tatgttgagt ttgttggtcc
     2761 accttgagtg agtaggtaga taacgttgaa gttgttaata tttccgataa attgttgaat
     2821 taaagtagga gtcataatca acaaaatttg tgggaaagta attgattgaa agacttggaa
     2881 cttattcgcc ccatcaattt cagcagcttc aatttgttct gcaggtaaat tcatgatgat
     2941 accagtagca acaagcattg ttacaggaat accaacccac atattaacaa agatgattga
     3001 gaatttcgcc cagaccggat cagaaaggaa tggaagggga tgtgctgaag aaataagtcc
     3061 ccattttatt aacaaggcgt taaccggtcc agcgtcactt aagaggtttc tcatgattaa
     3121 gagagagata aattgaggga cagccatggt aataacaaaa atagtacgcc acatcttttt
     3181 gaatttcaaa ccttttgtat taattagtaa ggcaagaata ataccaaaga agaaattcgt
     3241 tacagtagcg aaaacagccc aaattaatgt ccaagataga acagggaaga aagtactggc
     3301 catacgcccg ctaaagatat taccgaagtt agcaaaacca acccagtcaa ataaactttt
     3361 tggcggtaag tggttatgat catagttagt aaatgccaaa caaatcatat aaactaacgg
     3421 aaggattgta aatagaagca cccctattaa aggaatagac attaaagtca tatggaagcg
     3481 tccatctgtc aagcttttaa tatctttttt gaaggatgga atttctcttc cttcttcttt
     3541 caaggtcaat aaatgtgctg cgcttttaat gtttgaccag taaatatagg caaatacgca
     3601 acagaagata agcgaagcta atccaaaaat tagcattaac atagagttat caccttgtgt
     3661 cgcaacttgg attttaatac catccacagt tttagttgtc ataccttgtt cttgggtccc
     3721 taaggaaata agacctttta cagctgggat aacttggtaa acaaaggtaa ttaaaaacag
     3781 aatttcactg attaaaaata aaagtccctt gacaatttgt ttatttttga ggttagcaag
     3841 tcccataatt aagaatgata gtttagttgc taaatcacct ttttgaaaga cctcttttag
     3901 ggacatacca tcaagtgaga tcagtttttt attcataatg gtctccttta attgttattt
     3961 tacaaaataa gagatgggaa gcactctcaa ttgaaaggaa gagtactcac ctcatctcat
     4021 tactctagac gtactatttt gttttagcga tgtctttatc gaattgtttt agacgtttaa
     4081 ggtaatcgct agatttgatt ttaccactgt aagtatcgct aagaatagca gcactttctg
     4141 tccagaatgt tgacatttga ctcaacttag gcataaccgt tgtatattta tctgagctac
     4201 ccatttcgat aactgctttt gcaagttcat ctttttggac gctatcagaa gattgaattg
     4261 atgagttagc aggaacgata tgacgttttt cgaattgaat tttttgactt tcagcattag
     4321 ttagatatgc agcgagtttg tagctagctg agattcgttt agtgtttgaa ccagcaggtg
     4381 cttggttaac ggcataaagt ttaacgccca agaatgcttt ttgttgaact tctttgtcac
     4441 cgattttcat tgttgggtaa acagcaacac cgattttatc ttcaccgaca gcttttttag
     4501 cagcgtcgta atcccatggt ccactttcaa aagcatgaac agaaccatcg ccaaatttag
     4561 acattgtatt ttcagctgtc aagttgacaa aaccatcatt tttcttttga tctgcaatcc
     4621 atttaaggac agaaacacct gcttcattac cccagttagt gcctttagca tcttcaccag
     4681 atttaccaaa taaagtgtcg cctacagaaa ggaaaagagg acctgttaca tatgagttag
     4741 ctgctttaag ctgttgaccg aatttccctt tgcttgtaat tgtttcgtat gatttaacgt
     4801 cgtcagcagt taactttgtt ttattataat aaagaacttg agattcaata ccaaatggga
     4861 atgcataagt tttcccttta tattgtgcac cagtaagtga ttgtttagtg tcgtttttag
     4921 caatttcttt tgagtattgc tctggaattt cttggataac accagattct actaattgac
     4981 caagttggtc atgtggaagt gagaatacat cggctgcctt gcttgggtct ttttttacgt
     5041 tttcttgagc tttggagtca ttagactcaa tcatttttac agtaacgcct ttgttttcct
     5101 tctcgaattt tttaacaatt gctttataag acgcttttga atctgttggg acccaaagtt
     5161 tgatagttgt ttttgaagca gcatcaactg agttagagtg agtagcagca attgctcctc
     5221 ctgcaactac tgaaagagca gcagtactaa caagtaattt tttccaagta tttttttcca
     5281 ttttggttct cctcctagag aatattttaa ttacacctat attatgcaac cgtttgcaaa
     5341 aaaagtcaag tcttttttta aaaaaaataa gaaaaataaa aaaataatat caattaatac
     5401 taaatgagaa gattatttta gtgagagtat tacatttgca gagaaggcaa acgtttgttt
     5461 ttttcttaga ttagagtata atagacaaag gactatacca aaaggtataa aatgattaga
     5521 atgaagaggt cgtaggatgg taactataaa agatgttgct gccaaagctg gtgttaatcc
     5581 atcgacagta agtcgtgtac taaaagataa tgaatctatt tcaagtaaga cgaaagagcg
     5641 cgtgaaaaaa gctatggaag agctgggcta tgttcctaat gttgcagcac aaatgttggc
     5701 tagtggttta acgcaaaata ttggagttat ttttcctcca ctggttttac cagaccgctt
     5761 aagtgaaccc tttttcatgg agattctttc tacattaact aatgaagcaa aagagcatgg
     5821 gtttacagtt tctattgcaa caagtatgtc ttctgaggag ttgttagaac aagtaaaatt
     5881 aatgcatcta caaaaacgag tggatggttt tattgttctc tattcggatc aaaaagatcc
     5941 tatcagagag tatttattaa aatataaact gccctttgtt gttgttggtg ctgctaatgg
     6001 ctatgaaaat gagattactt atattgataa tgataatcag ctgatgggga aatctgccat
     6061 tgattatctc tataaaaaag gacataaaag catccagttt gttacagatg atttaaattc
     6121 agaagtatca gaagagcgct atttagggta ttttaaaggt gctagaaagt taggtttaaa
     6181 ccaaaagcca gctcttttat ttgacagagg caatccgcaa gttttagagg aatttataaa
     6241 tagagtcaaa gaagaagaaa caactgcttt aattgttatt ggtgatactg tctctgttag
     6301 agtaatgcaa tttctttctt tctataaatt aaaagttcca gatgacattt ctatcatgac
     6361 ctttaataat tctctcttct cacatctgat tcatccttac ctaagcacat ttgatattaa
     6421 tgttaataat ctaggaagga caagcgttcg tcgtctgatt gacatcatta aatctccaga
     6481 caaagtcttt agtgagacta ttattgtgcc ttttacttta gaagagcgtg aaagtgtccg
     6541 agatatttct aaaaataata attaagctat gatataaaga cttctatcaa ttttcgatag
     6601 aagttttttt aaattatgct tgacttatgc aatcgcttgc gttaagatgg aggagtaaag
     6661 taagtaatcg aaaaggagaa tcaatatgaa aaaacgtgca agtggtgtct taatgcacat
     6721 cacttctcta ccaggtgatt taggtattgg aacatttgga cgagaagcct acgcttttgt
     6781 tgattttttg gtcgaaactg atcaaaaatt ttggcaaatt cttcccttaa caacaacaag
     6841 cttcggggac tctccttacc agtctttttc tgcagtagct ggtaatacac atttaattga
     6901 ttttgatttg ctaacgcttg aaggtttcat ttcgaaagat gactaccaaa atattagttt
     6961 tggacaggat ccagaagttg ttgattatgc tggtctgttt gaaaaacgcc gtccagtttt
     7021 agaaaaagca gttaaaaatt tcttgcaaga agagagagct acgagaatgc tatctgattt
     7081 cttgcaagaa gaaaaatggg taactgattt tgctgaattt atggcgatca aagaacattt
     7141 tggtaataag gcgcttcaag aatgggatga caaggctatt atacgccgcg aagaagaagc
     7201 cttagcagga tatcgtcaaa agcttagtga agtgataaaa tatcatgaag taacgcaata
     7261 tttcttttac aaacaatggt ttgagttaaa agaatatgct aatgataaag ggattcaaat
     7321 tatcggtgat atgccaatct acgtttctgc cgatagtgta gaagtttgga caatgcctga
     7381 actgtttaaa ttagattgcg acaaacaacc acttgctatt gctggtgtac cagcggatga
     7441 tttttctgac gatggtcagc tttggggcaa tcctatttac aattgggatt accacaaaga
     7501 gtcagatttt gactggtgga tttatcgtat tcaatctggt gttaagatgt atgactattt
     7561 gcgtattgat cattttaaag gtttttcaga ttattgggaa attcgaggag attatcaaac
     7621 agctaatgat ggctcatggc aaccagctcc cggtccagaa ttatttgcaa ctatcaaaga
     7681 aaaattaggt gatttaccta ttattgctga gaatttaggc tatattgatg agagagcaga
     7741 gagattactg gctggaacag ggttcccagg tatgaaaatc atggaattcg gtttttatga
     7801 tacaacagga aatagtattg atattcctca taactacaca gaaaatacaa ttgcatatgc
     7861 tggaactcat gataacgaag ttattaatgg ctggtttgaa aatcttacag tcgaacagaa
     7921 agcttatgca gaaaattaca tgcgtcgttt gccaaatgag cccattactg agactgtact
     7981 gcgtaccttg tatgcaaccg ttagtcaaac aacgattaca tgtatgcaag atctactaga
     8041 taaacctgca gatagccgta tgaacatgcc aaataccgtt ggaggtaact ggcaatggcg
     8101 tatgagaaaa gaagacttaa cagaaaatcg caaagcattc ttaaaagaga ttacaactat
     8161 ttataataga gggaataaat aaggaatatt atcatgacaa gaaattttac aacttacgtt
     8221 ggacaacaag gaaaagtatt atcagaatta acaaatgaag agatttacgt tgaattgtta
     8281 aattttgtaa aagaagaagc tgccgctaaa tcaaaaaata gctctcaacg taaggtatac
     8341 tatatttcag ctgagtttct aattggtaaa ttactttcaa ataatttgat taacttaggg
     8401 atttataaag atgtcaaaaa agagttagaa cttgtaggta aatcaattgc tgaaatagaa
     8461 gatgtagagc cagaaccatc acttggtaat ggtggtttag ggcgtttagc atcatgtttt
     8521 attgactcga tttcttcttt aggtattaat ggtgaaggtg ttggtttaaa ctatcactgt
     8581 gggcttttca aacaagtatt tcgcaacaat caacaagaag cagaagctaa ctactggatt
     8641 gagaataatt cttggttagt accaacagat atttcttatg atgttccttt ccgtgacttt
     8701 actttaaaat cacgcctaga tagaattgat gtattaggtt ataagaaaga tactaaaaat
     8761 tatttgaacc tttttgatat tgatggactt gattataacc ttattgaaaa aggtatcact
     8821 tttgataaga cagaaatcaa gaagaattta accttgttct tgtatccaga tgattcagat
     8881 aaaaatggag aattattacg tatctatcag caatatttca tggtctcaaa tgctgctcaa
     8941 ttattaattg atgaagcaat cgagcgtggt tcaaaccttc atgatttagc tgaatatgct
     9001 tatgttcaaa ttaatgacac tcacccatct atggtcatcc ctgagttgat tcgtttattg
     9061 acagaaaaac atggttttga atttgacgaa gctgtgtcag ttgttcgcaa tatggttggc
     9121 tatacaaacc ataccatcct tgcagaagct cttgaaaaat ggcctttaga gtatcttaat
     9181 gaggtagtac cccacctagt aacaatcatt aaaaaattag atcaaatgat tcgtgaagag
     9241 caaacaaatc cagaggttca aattattgat gaagctggac gtgttcacat ggcgcatatg
     9301 gatatccact tctcaacaag cgttaatggt gtagcagcat tacatacaga aatccttaaa
     9361 aatagtgaat taaaggtttt ctatgatatt tacccagata agtttaacaa taagacaaac
     9421 ggtattacat tccgtcgctg gttggagttt gctaaccaag atttagcaga ttacttgaaa
     9481 gaactaattg gggatagcta tcttactgat gcgactcagc tcgaaaaatt gttaacttat
     9541 gctgatagca atgaagttca tgataaatta gctgctatta aatttaaaaa caaactggct
     9601 cttaaacgtt accttaagga aaataaaggt attgaattgg atgaatactc aattattgat
     9661 acacaaatca aacgattcca cgaatataag cgccaacaaa tgaatgcttt atatgttatt
     9721 cataaatact tagaaattaa acgtgggcat ttcccatcac gtaaattaac agttattttt
     9781 ggtggtaaag ctgctccagc ctatactatt gcacaggata tcattcactt aattctctgc
     9841 ttatcagaat taattaataa tgatccagaa gttaataaat accttaatgt ccatttagta
     9901 gaaaattaca atgtgacagt agcggaaaag ttaatccctg caacagatat ttcggaacaa
     9961 atttcattgg cttccaaaga agcatctggt acaggtaaca tgaaatttat gttgaatggt
    10021 gctcttactc ttggtactat ggatggggca aatgttgaaa tagctgaatt agctggcaaa
    10081 gagaacatct acacatttgg taaagactct gatactatca ttaatcttta tgaaacatct
    10141 ggttatcgtt caaaagatta ctatgataaa gacaaagtaa ttcgtgaagc tgttgatttt
    10201 atcattagtg atgatatagt gtcactcggt aatgcagaac gactaaagag attgcatgat
    10261 gagttagtag gtaaagattg gttcatgaca ttgattgatt tgaaagaata cattgctgtt
    10321 aaagaacaag tgcttgctga ttatgaagat tatgaatcat ggaataagaa agttatacac
    10381 aatattgcta aagcagggtt cttctcatca gatcgtacaa tcgaacaata taatcaagat
    10441 atttggcatt caaattaaaa agtctctaaa agtaaggttt tcaaccttac ttttttcttg
    10501 tctaataaaa ctataagtat ttatttttgt ccgtagactt ta
//
LOCUS       DATRUK010000065         8798 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.065, whole genome shotgun sequence.
ACCESSION   DATRUK010000065 DATRUK010000000
VERSION     DATRUK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.065"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(11..1438)
                     /locus_tag="VBE34_001661"
     CDS             complement(11..1438)
                     /locus_tag="VBE34_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO7264362.1"
                     /translation="MTKEYQNYVNGEWKSSVNQIEILSPIDDSSLGFVPAMTREEVDH
                     AMKAGREALPAWAALTVYERAQYLHKAADIIERDKEEIATVLAKEISKAYNASVTEVV
                     RTADLIRYAAEEGIRLSTSADEGGKMDASTGHKLAVIRRQPVGIVLAIAPYNYPVNLS
                     GSKIAPALIGGNVVMFKPPTQGSVSGLVLAKAFAEAGLPAGVFNTITGRGSEIGDYIV
                     EHEEVNFINFTGSTPVGQRIGKLAGMRPIMLELGGKDAGIVLADADLDNAAKQIVAGA
                     YDYSGQRCTAIKRVLVVEEVADELAEKISENVAKLSVGDPFDNATVTPVIDDNSADFI
                     ESLVVDARQKGAKELNEFKRDGRLLTPGLFDHVTLDMKLAWEEPFGPILPIIRVKDAE
                     EAVAIANKSDFGLQSSVFTRDFQKAFDIANKLEVGTVHINNKTGRGPDNFPFLGLKGS
                     GAGVQGIRYSIEAMTNVKSIVFDMK"
     gene            complement(1588..3321)
                     /gene="ptsP"
                     /locus_tag="VBE34_001662"
     CDS             complement(1588..3321)
                     /gene="ptsP"
                     /locus_tag="VBE34_001662"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEO7264363.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVEDTNAEEARL
                     DVALQASQDELSVIREKAVESLGEEAAAVFDAHLMVLSDPEMINQIKETIRAKQVNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITERVQDGQ
                     LIAVNGITGEVIIEPTEAQISAFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNENGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAIFEEEKANLLADGVAVAEGIEVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALSECSTMEEV
                     IELQKEYVDFD"
     gene            complement(3326..3589)
                     /locus_tag="VBE34_001663"
     CDS             complement(3326..3589)
                     /locus_tag="VBE34_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEO7264364.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAIEETMTKEGLA"
     gene            3972..4196
                     /locus_tag="VBE34_001664"
     CDS             3972..4196
                     /locus_tag="VBE34_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEO7264365.1"
                     /translation="MAAITVFSKNNCMQCKMTKKFLDQHGADFEEINIDEKPEKIEYV
                     KNLGFSAAPVIEAGNVVFSGFQPSKLKELV"
     gene            4274..6433
                     /gene="nrdE"
                     /locus_tag="VBE34_001665"
     CDS             4274..6433
                     /gene="nrdE"
                     /locus_tag="VBE34_001665"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO7264366.1"
                     /translation="MSLKNIGDVSYFRLNNEINRPVNGQIPLHKDKEALTAFFKENVQ
                     PNSKAFDSITDKIAYLLKYDYLEEAFLNKYRPEFIEELSTKLFDKKFRFKSFMAAYKF
                     YQQYALKTNDGEYYLESIEDRVLFNALYFADGDEELATDLALEMISQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNAIGRSINSALQLSRIGGGVGISLSNLREAGAPIKGF
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLDVFHPDIISFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARNNQEMYLFSPYSIEREYGVPFSYIDITEKYDELVANPNI
                     TKTKINARDLETEISKLQQESGYPYIINIDTANRTNPVDGKIIMSNLCSEILQVQKPS
                     LINDAQEYLEMGTDISCNLGSTNVLNMMTSPDFGKSIKTMTRALTFVTDSSNIEAVPT
                     IKNGNAQAHTFGLGAMGLHSYLAKNHIEYGSPESIEFTDIYFMLMNYWTLVESNNIAR
                     ERQTTFVGFEKSKYADGTYFDKYVSGKFVPQSDKVKSLFTNHFIPEAKDWENLRYAVM
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLATDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSMTLFLRSELPKELYEWKTESKQTTRDLS
                     ILRNYAFNKGVKSIYYIRTFTDDSSEVGANQCESCVI"
     gene            6636..7595
                     /gene="nrdF"
                     /locus_tag="VBE34_001666"
     CDS             6636..7595
                     /gene="nrdF"
                     /locus_tag="VBE34_001666"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO7264367.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSAQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKSEIEEIFEWTNNNEFLQEKARIINDIYANGNALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMHDDDYNYGL"
     regulatory      7680..7778
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7835..8398
                     /gene="thiT"
                     /locus_tag="VBE34_001667"
     CDS             7835..8398
                     /gene="thiT"
                     /locus_tag="VBE34_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEO7264368.1"
                     /translation="MSKNNNTTCLIETAIFAALAMALSMIPDFASWFTPSFGAIPLIL
                     FALRRGTKYGLFAGLIWGLLHFVLSKVYYLSLSQVFIEYILAFISMGLAGVFSAKFKD
                     ALSSSSKTKALSLALSGAILATFVRYVWHYIAGVIFWASYAPKGMSATLYSLSVNGTA
                     GLLTLFFVVISIIILVISYPSFFLPKK"
     gene            complement(8483..>8798)
                     /locus_tag="VBE34_001668"
     CDS             complement(8483..>8798)
                     /locus_tag="VBE34_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEO7264369.1"
                     /translation="LGLHIYFLKVYRNIRGIKMTVAPTLKELKNNVLQLDEAELESNY
                     KMCFDIVMNLSGFIFPTIYFVLFFISFVFQKVEIVAIIITTSIHIYILIKSLKAARHF
                     YR"
BASE COUNT         2786 a         1682 c         1462 g         2868 t
ORIGIN      
        1 cgttttacat ctatttcata tcaaaaacaa tcgattttac atttgtcatt gcttcaattg
       61 aatatctgat accttgaaca cctgcaccag atcctttgag tcctaagaat gggaagttat
      121 caggaccacg tccagtctta ttgttaatgt gaactgtacc aacttcaagt ttatttgcta
      181 tatcaaatgc tttttggaaa tcacgtgtaa agactgatga ttgtaatcca aaatcagatt
      241 tgttggcaat agcaacagct tcttctgcat ccttgacacg aataattggg agaattggtc
      301 caaaaggctc ttcccaagct agtttcatat ctaaagtaac atgatcaaac aatcctggag
      361 ttaatagacg accatcacgt ttaaattcat tcaattcttt cgcacctttt tgacgtgcat
      421 ctactactaa gctttcaata aagtcagctg aattatcatc aataaccggt gtcaccgttg
      481 cattatcaaa tggatcacct actgataatt ttgctacatt ttcagatatt ttttctgcca
      541 attcatctgc aacttcttca acgacaagca cacgcttaat tgccgtacag cgttgtccag
      601 agtaatcata agcacctgca acgatttgtt tagcagcgtt atcaaggtca gcatcagcta
      661 agacgatacc tgcatcctta ccgcccaact caagcataat tggacgcatt cctgccaact
      721 taccaatacg ttgtccaact ggcgttgatc ctgtaaagtt aataaaatta acttcttcat
      781 gctcaacaat gtaatctcca atctcagaac cgcgtcctgt aatagtatta aagacacctg
      841 ctggaagacc tgcttctgca aaagcttttg ctaaaacaag tcctgagact gaaccttgtg
      901 ttggtggttt aaacatcaca acgtttccac caattagagc tggcgcaatt tttgatcctg
      961 agaggttaac agggtaatta taaggtgcga ttgctaaaac gatacctact ggttgacgac
     1021 gaataacagc caacttatga cctgttgaag catccatttt tccaccttcg tcagctgaag
     1081 ttgataaacg aattccttct tctgctgcat aacgaataag atcagctgtc cttacaacct
     1141 cagttactga agcattgtaa gctttagaaa tttcttttgc taaaacagta gcaatttctt
     1201 ctttatcacg ttcaataatg tctgcggctt tatgaaggta ttgtgcacgt tcatatactg
     1261 ttaaagcagc ccaagctggt aaagcctcac gacccgcttt catagcatga tcaacttctt
     1321 ctcgagtcat cgctggcacg aatcccaatg aagaatcatc aattggtgac aaaatctcaa
     1381 tctgattaac agatgatttc cattcgccat tgacataatt ttgatattct tttgtcaaag
     1441 taaatctcct tttacattgt aataggttta ttctatcact ttatctattt atttcaatta
     1501 tctgacatat ttttcttaga aacttaacaa tatccaaact ttcaataaaa aagtagagat
     1561 tttttaatcc ctactttttt attattctca gtcaaagtca acgtattctt tttgaagttc
     1621 gataacttct tccatagttg aacactctga aagagcacga ttagcgtatt cttccatttt
     1681 agctgtgtcc aattttttca ttaagctacg tgtacgaagt actgaggttg ctgacattga
     1741 gaattcatcc aatcccatac caacaagaag tggaaccgca gtttgatcac cggccatctc
     1801 accacacata cctgcccatt taccttcagc atgagcagct ttgataacgt tgttaatcaa
     1861 acgaaggatt gatggattgt atggttgata gaggtatgaa acttgctcat tcatacggtc
     1921 cgcagccatt gtgtattgga taagatcatt agtaccaatt gagaagaaat caacttcttt
     1981 tgcaaattgg tctgcaagca tagctgccgc tgggatttca atcataatac caacttcaat
     2041 gccttcagct actgctacac cgtcagctaa caagttcgct ttctcttctt caaatatagc
     2101 tttagcagca cggaattctt taagcaaagc aaccattggg aacatgatgc gtagctgtcc
     2161 atgaactgaa gcacgaagaa gtgctcggat ttgtgtgcgg aacattgcat caccagtttc
     2221 agaaattgaa atacgcaatg cacggaatcc taagaatggg ttcatctctt ttggaagatc
     2281 aaagtaagga agctctttat ctccaccgat atccattgta cgaacaacaa ctggtttacc
     2341 attcataccc tcaagaactg ctttatatgc ttcatattgc tcatcttctg ttgggaaatc
     2401 ttgtgaatcc atatataaga actctgtacg gtaaagacca actgcttctg ctccattttc
     2461 gttaacaccc tcaacatctt taggtgtacc gatattagca gctaattcaa aatgtttacc
     2521 atcagcagta actgtttgtg catccttaag aagtgcccat tcagcttttt gcttggcata
     2581 agcttcacct gctgccttga aggctgaaat ttgagcttca gttggttcaa tgataacttc
     2641 accagtaata ccattaacag caatcaactg accatcttga acacgttcag taatatcatt
     2701 tgtaccaaga acagctgcga tttcgagtgt acgtgccata atagctgagt gacttgtacg
     2761 ccctccaata tttgttacaa aagctttaac aaattgttta tttaattgag ctgtatcaga
     2821 aggtgtcaaa tcgtgtgcaa taacgattga ttcttcattg atagttgccg ggtttggtaa
     2881 tttaacaccc aaaagatgtg ctaagacacg tttggcaaca tcacggatat ctgctgcacg
     2941 ttcttgcatg tatgggttat cttccatacc ttcaaagata gtgatgaaca tatcagtcac
     3001 ttctttaaga cctgtttctg catttacttg ttttgcacga atagtctctt taatttgatt
     3061 aatcatttct gggtcagaaa gaaccattaa atgagcatca aatacagctg cggcttcttc
     3121 gcctaagctt tctactgcct tctcacggat aacagaaagc tcgtcttgtg aagcttggag
     3181 tgcaacatca aggcgagctt cttctgcgtt tgtatcttcg actgttacag tctcaaatga
     3241 caaatccggt tggacaagta gatatgcttt agcaactgca acaccatcag aggctgcgat
     3301 tcctttaagc atttctgtca ttttcttatg ccaatccttc ttttgtcatt gtttcttcga
     3361 tagctgcaat tgcatcatct gcatctgcac cttctgctga aatagtaaca tcagcacctt
     3421 gaccaacacc aagactcata acacccatga ttgatttaag gtttactgct ttacctttgt
     3481 aatcaagcgt gatgtctgaa gcaaatttgc tagctgtttg gacaagcaaa gtagctggac
     3541 gtgcgtgaat acctgtttct gcaacaatgt ggaaatcttt tgaagccata ttatggttct
     3601 ccttttgtca tttgtaaata tttgagtcac ctattggtaa cccttacaag ttaaaagtat
     3661 aacatattgc tttttatttt tcaagattaa atgtctcaaa cagaaaaaaa cttgaatgtt
     3721 ttccacagct tttcatatta aaaatgagag tcttagaatt taaaaataga attctaaaaa
     3781 tgattttttt atgttcttct gaaaggaatt ggaaaacagg ctgaaagctt ttaaactaac
     3841 cctaaaaacg aaatatagtg tctcatttta ttttaagaca ctatatattg tgttttgtta
     3901 gaaataaaac acttgctttt tccattcttt ttttatatgc tattaccatg aaaaaaaatt
     3961 aggaggacaa catggcagct attactgttt tttcgaaaaa caactgtatg caatgtaaaa
     4021 tgactaagaa atttcttgac cagcacggcg ctgattttga agaaattaat attgatgaaa
     4081 aaccagaaaa aattgaatat gtgaaaaatc tcggcttttc tgcggctcca gtgattgaag
     4141 ctggtaatgt tgttttttca ggtttccaac caagcaaact aaaagaactt gtttaagcaa
     4201 attagagtct gggaataaag caattaattg ctttattccc attttgtatt gttaagttaa
     4261 gaggataaat tcaatgagtt taaaaaatat tggcgatgta tcttactttc gcctaaataa
     4321 tgaaattaac cgtcctgtta atggacaaat cccactccat aaagataaag aagccttaac
     4381 tgcttttttc aaagaaaacg tacagcctaa ttcaaaagct tttgactcaa tcactgataa
     4441 aattgcttac ctccttaagt atgattatct tgaagaagct ttcctcaata aatatcgtcc
     4501 tgaatttatt gaagaattat ctaccaaact cttcgataaa aagtttcgtt ttaaatcctt
     4561 catggcagcc tataagtttt atcagcagta tgcattaaaa acaaatgatg gagaatacta
     4621 tttagaaagt attgaagatc gcgttctctt taacgctctt tattttgcag acggtgatga
     4681 agaactagca actgacttag ctctagaaat gatttcacaa cgctatcaac ctgcaacacc
     4741 aagtttttta aatgctggtc gaagtcgtcg cggagaatta gtctcatgtt tcttaatcca
     4801 agtaactgat gatatgaatg ctattggtcg ttcgattaat tcagctcttc aattatctcg
     4861 tattggtggt ggggttggaa tatcacttag taaccttcgt gaggctggag ctcctatcaa
     4921 aggttttgct ggtgctgctt ctggagtcgt tccagtcatg aaattatttg aagacagttt
     4981 ttcttactcc aaccaacttg gacaacgcca aggtgctgga gttgtttacc ttgacgtttt
     5041 ccatccagat attatttcat tcctatcaac taaaaaagaa aatgctgatg aaaaagtacg
     5101 tgttaaaaca ttgtcattag ggattactgt acccgataaa ttctatgaac tagcacgtaa
     5161 caatcaagaa atgtacttgt ttagtcctta tagtatcgaa cgtgaatatg gtgttccttt
     5221 cagctatatt gatattaccg aaaaatatga tgaattagtc gctaacccta atatcactaa
     5281 aacaaaaatc aatgctcgtg atttagaaac agagatttct aaacttcaac aagaatctgg
     5341 ttatccttac atcatcaata ttgacactgc taatcgtact aatcctgttg acggaaaaat
     5401 tattatgtca aatctttgct ctgagatttt gcaagtacaa aaaccaagtc ttatcaatga
     5461 tgctcaagaa taccttgaaa tgggaactga tatttcttgt aaccttggct caactaatgt
     5521 tttaaatatg atgacatctc ctgattttgg aaaatcaatt aaaacaatga ctcgtgctct
     5581 aactttcgtt actgactcat caaatattga agctgttcca actattaaga acggaaatgc
     5641 tcaagcacat acatttggac ttggagcaat gggactccac tcctatttgg caaaaaatca
     5701 tatcgaatat ggtagtccag aatcaattga atttactgat atttatttca tgctaatgaa
     5761 ctactggacc ttagtagaat caaacaatat tgcccgcgaa cgtcaaacaa catttgtagg
     5821 ttttgaaaaa tcaaaatacg ctgacggtac atactttgat aaatatgtat ctggaaaatt
     5881 tgttccacaa tctgacaaag taaaatccct atttactaat cattttatcc ccgaagctaa
     5941 agattgggaa aaccttagat acgcagttat gaaagacgga ctatatcatc aaaatcggct
     6001 tgcagttgct ccgaatggtt ctatttcgta tatcaacgat tgttcagcat ctatccaccc
     6061 tattacacaa cgtattgaag agcgtcaaga gaaaaaaatc gggaaaattt attatccagc
     6121 taatggctta gctacagata ctatccctta ttacacttct gcctatgata tggatatgcg
     6181 taaagtaata gacgtctatg ccgcagcaac tgagcacgta gaccaaggtc tctcaatgac
     6241 actcttttta cgaagcgaat taccaaagga gttgtacgag tggaaaacag aaagtaagca
     6301 gactactcgc gacctttcaa tcctacgtaa ctatgctttc aacaaaggtg tgaaatcaat
     6361 ctactatatc cgtactttta cagatgatag tagcgaagtc ggagctaatc aatgcgaaag
     6421 ttgtgtcatc taacgaagaa actctactta aaacatcaga aactagactg agataaacta
     6481 tctcagtttc atgatgttat cttattttct aaggtatttt catctttatt tttctaaaaa
     6541 tcgataatac ttgaaatatt gctagatcag aattctatat tctactcttc ttccatcatc
     6601 attatataaa aacctattta ccattggaga aaaccatgac aacttactac gaagctataa
     6661 actggaacga aattgaagat gttattgata aatcaacttg ggaaaaacta accgaacaat
     6721 tttggctcga tacacgtatc cctttatcaa atgacttaga cgattggcgc aaactttccg
     6781 ctcaagaaaa agatcttgtt ggcaaggttt ttggaggctt aaccctactt gataccatgc
     6841 aatcagaaac tggtgttgaa gctattcgtg ccgatgttcg cacgcctcac gaagaagctg
     6901 tcttaaacaa tattcaattc atggaatctg ttcacgctaa atcttattct tcaattttct
     6961 caactttaaa tactaaatca gaaattgaag aaattttcga gtggactaat aataatgagt
     7021 tccttcaaga aaaagcacgt attatcaatg acatttatgc taatggaaat gcccttcaaa
     7081 aaaaggtggc ttccacctac ctcgaaactt tcctttttta ttctggcttt ttcacacctc
     7141 tttactattt gggaaataat aagttagcaa atgttgctga aatcattaaa ttaattattc
     7201 gtgatgaatc tgtacatggt acttatatcg gttacaaatt ccagcttggt tttaacgaat
     7261 taccagaaga tgagcaagag aattttcgtg attggatgta tgacctcctt tatcagctgt
     7321 atgaaaacga agaaaaatac accaagacac tttatgatgg cgtaggatgg actgaagaag
     7381 ttatgacctt tttacgctac aatgctaata aagctcttat gaatttagga caagatcctt
     7441 tattcccaga tacagcaaat gatgtcaacc caattgttat gaatggtatt tcaacaggaa
     7501 catcaaacca tgacttcttc tctcaagtag gtaatggtta cctacttggt agcgttgaag
     7561 ctatgcatga tgatgactat aactatggat tataagaaaa agacactgat ttagtgtctt
     7621 tttctagtta aatacaaatt tcttgacaag caaacccttt caagatagaa tgttagagta
     7681 aatacacaag ggagtgcctt gagctgagat tgcagatatg caaaatcctc taacctgatc
     7741 tcgttaggac gagcgtagga attgtggttt gaattggtta aacatgacaa atgacaaaag
     7801 aatctccttt gctgtataaa aaaggagatt ttttatgtca aaaaataaca acactacctg
     7861 tcttattgaa actgctattt ttgcagcgct tgctatggct ttatcaatga ttcctgattt
     7921 tgcgtcatgg ttcactccct cttttggagc aattcctctg atactttttg cattacgtcg
     7981 tggtacaaaa tacggtctat ttgctggctt aatttggggc ttattgcact ttgttctttc
     8041 caaagtatat taccttagtt tatcccaagt ttttatagaa tacatcttag catttataag
     8101 tatgggactt gcaggcgtat tttctgctaa atttaaagat gcacttagca gcagctctaa
     8161 aactaaagct ttatcattag cattatctgg agcaatatta gcaacatttg ttcgttatgt
     8221 ttggcattat atcgctggtg ttattttttg ggctagttat gcaccaaaag gtatgtctgc
     8281 tacattatac tcattaagcg tcaatggtac tgctggtttg ttaactctat tttttgtagt
     8341 cattagtatt attattttag ttatcagcta ccctagcttc tttttaccta agaaatagaa
     8401 aaaataaaag gactgatttt cagagaatca atccttttat tttacactaa tataagtttt
     8461 ttgataaaaa atctagctac tactatctat aaaaatgacg tgctgctttt aaagacttaa
     8521 tgagaatgta gatatgaata gatgttgtta taataattgc tacaatctcc actttttgaa
     8581 aaacaaatga gataaagaat aatacaaaat agatcgtagg gaaaataaaa ccacttaaat
     8641 tcatgacaat atcaaaacac atcttataat ttgattccaa ctctgcttcg tccaattgta
     8701 agacattgtt tttcaactct tttaaagttg gagcgactgt catttttata cctcggatat
     8761 tccgatatac ctttaaaaaa tatatatgta aacccaat
//
LOCUS       DATRUK010000066         8724 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.066, whole genome shotgun sequence.
ACCESSION   DATRUK010000066 DATRUK010000000
VERSION     DATRUK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8724
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.066"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..820
                     /locus_tag="VBE34_001669"
     CDS             <1..820
                     /locus_tag="VBE34_001669"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEO7264370.1"
                     /translation="ENPSNIGFDKDYYVSNLFFENSTRTHKSFEMAELKLGLKTIEFN
                     ADTSSVNKGETLYDTILTMSALGLDVCVIRHPDIDYYKELIASPNIHSAIVNGGDGSG
                     QHPSQSLLDLVTIYEEFGYFKGLKIAIVGDLTHSRVAKSNMQVLKRLGAEIFFSGPKE
                     WYSSQFDEYGQCLPIDQLVDQIDVLMLLRVQHERHDGKGVFSKESYHQQFGLTKERYK
                     HLRDTAIIMHPAPVNRDVEIASDLVEADKARIVKQMSNGVYARIAILEAVLNSR"
     gene            834..1910
                     /gene="carA"
                     /locus_tag="VBE34_001670"
     CDS             834..1910
                     /gene="carA"
                     /locus_tag="VBE34_001670"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEO7264371.1"
                     /translation="MKRLLLLEDGSVFEGEAFGADVGTSGEIVFSTGMTGYQESITDQ
                     SYNGQIITFTYPLIGNYGINRDDYESIRPTCKGVIVYEWAEYPSNWRQQMTFDEFLKL
                     KGIPGISGIDTRALTKIIRKHGTMKACLINEGNSIHEALENLQKSVLLNNQIEQVSTK
                     LAYASPGVGKNIVLVDFGLKHSILRELSQRQCHITVVPHTTTAQEILNLNPDGVLLSN
                     GPGNPEQLPNALQMIQEIQGKIPIFGICMGHQLFAKANGAKTYKMTFGHRGFNHAVRH
                     LQTGQVDFTSQNHGYAVSREDFPEALFITHEEINDKTVEGVRHKYYPAFSVQFHPDAA
                     PGPHDTSYLFDEFINMIDDFQQKS"
     gene            1941..5123
                     /gene="carB"
                     /locus_tag="VBE34_001671"
     CDS             1941..5123
                     /gene="carB"
                     /locus_tag="VBE34_001671"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEO7264372.1"
                     /translation="MPKRTDIRKIMVIGSGPIVIGQAAEFDYSGTQACLSLKEEGYQV
                     VLVNSNPATIMTDKDIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMAL
                     SKNGILEELNVELLGTKLSAIDKAEDRDLFKQLMEELNQPIPESEIVNSVEEAIQFAE
                     QIGYPLIVRPAFTLGGTGGGMCDNQEQLVDITTKGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDAADNALVVCNMENFDPVGIHTGDSIVFAPAQTLSDVENQLLRDASLDIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPITKTTYAMFEPALDYVVAKMPRFPFDKFESGDRKLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVDHIKIADLDNVSDDVLLEKIRKAEDDRLFYLAEALRRHYSIEKLAS
                     LTSIDSFFLDKLRVIVELEDLLSKNRLDINILKKVKNKGFSDKAIASLWQINEDQVRN
                     MRKEAGILPVYKMVDTCAAEFDSATPYFYSTYAVENESLISDKASILVLGSGPIRIGQ
                     GVEFDYSTVHSVKAIRESGFEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIDL
                     EKPEGVILQFGGQTAINLAKDLNKAGVKILGTQLEDLDRAENRKQFEATLQALNIPQP
                     PGFTATTEEEAVNAAQKIGYPVLVRPSYVLGGRAMKIVENEEDLRHYMTTAVKASPDH
                     PVLIDAYLIGKECEVDAISDGQNILIPGIMEHIERAGVHSGDSMAVYPPQTLSETIIE
                     TIVDYTKRLAIGLNCIGMMNIQFVIKDQKVYVIEVNPRAGRTLPFLSKVTHIPMAQVA
                     TKVILGDKLCNFTYGYDLYPASDMVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     INLQKALYKAFEAAYLHMPDYGNIVFTVDDTDKEEALELAKVYQSIGYRIYATQGTAI
                     YFDANGLETVLVGKLGENDRNHIPDLIKNGKIQAVINTVGQNNIDNHDALIIRRSAIE
                     QGVPLFTSLDTAHAMFKVLESKAFTLKVLD"
     gene            5249..5572
                     /locus_tag="VBE34_001672"
     CDS             5249..5572
                     /locus_tag="VBE34_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264373.1"
                     /translation="MLKTRTHINELETKIALDLVHRYLGIQQLVFSKWRHSLETGSWS
                     SIITINGANIINYIVDDISDSDSISGIICHPKSFHLRPREKKEPIQSIDELDIIWSLN
                     REENE"
     gene            5569..6039
                     /locus_tag="VBE34_001673"
     CDS             5569..6039
                     /locus_tag="VBE34_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HEO7264374.1"
                     /translation="MIPDSSFKVLSDMAYNIEKSKAESNGHLKIIEGSQIIDFHQKRS
                     FMVLEVNDNIDNGMQAMACQSLDESNHIVIAYSGTNAFNKKDILTDIDSVVLGKNNKL
                     LTCPSSITLREDGISLPCKNPSQITTSKLFAQKIKMNYPDATLWLTGHGKCSVR"
     gene            6017..8491
                     /locus_tag="VBE34_001674"
     CDS             6017..8491
                     /locus_tag="VBE34_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage infection protein"
                     /protein_id="HEO7264375.1"
                     /translation="MVNVQSGNISNYQKNLLDSATNFQNIFPALVNQYSSSITANESL
                     KKSLEASDNMFNDLMTTQTNTGKDLSSLIEQRHQDSISYEAFSASLLEMNNELLEKQL
                     SDIITQAQKDQETLSSQLNSIMGDDNDNDHNHKENSSAYLNVARQKIQELSEALKSQD
                     NIAKDQSEQLDKIVREGLASYFAKNNKDNITLLELLKSHSTNEKTLKDFKVKVADFTN
                     SLISSIPSLNLSELHLTQEEKAIQFTSSDSEIIKKVSNERTLYLNTNLLNRLFEARKN
                     RDEAKNKVNQLKLPSSSTRTGEQIVSVESNNPDYRVDTWTVNGKQTRTLDPSQNNNII
                     INSSYQGELSNSSITKPSYTITIGNQKQIVQNDDGEIQRSYLEAEATYQRTLQEVIDA
                     YNTTHDLVAKYYIISDGEEPQNLFDQFLNQSVNDTMVDLVKNGRTKYLMDENTADAQQ
                     KVKDVMEEVENSQDELADQMAKVTETNVRLTDTIKKQLETLQTINMKVQNITQDQSKV
                     NDSQKTTDQQLSDLKNQLDGLMTSATGVKDMSKSNSQEADQVNQIFTSFNKDVQDAKN
                     SGNKLSTDATDLMANFQKELANNGDFVASFSKVFNTAYKNGVPNDILLNFLSRPVAES
                     ASAVRATENTYRPFTWILLLEVVSLFIAYIFATQNLIKKLTDRYNVNRWLQTDFLNII
                     VISGLSLVIGLALGVISSRSLHVMPEYVPSWFLVMTLFSFLLIHSQYFFIKNFKAVGM
                     GLALFMIISFVYLSNAVGTVATVSGFPKLLKAINPLSILENQLSSYFDNVTTGFIFLI
                     LVLLVDVAFIIMNIFITLNFEAKVKE"
     gene            8500..>8724
                     /gene="essA"
                     /locus_tag="VBE34_001675"
     CDS             8500..>8724
                     /gene="essA"
                     /locus_tag="VBE34_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HEO7264376.1"
                     /translation="MKLKRFLACLLILSCMSQTSVFSDGLKNNDIHFDTKRLESDSQK
                     NSGFQTELTKDLFKKEDLKKLDKNRQSKVSL"
BASE COUNT         3043 a         1404 c         1632 g         2645 t
ORIGIN      
        1 agaaaatcct agcaacattg gttttgataa agattactat gtgtcaaatc tcttttttga
       61 gaattcaaca agaacacata agtcatttga aatggcagaa ctcaaattag gattaaaaac
      121 aattgaattt aatgctgata caagttctgt taataaagga gaaacccttt atgacactat
      181 cttaacaatg agtgctctag gattggacgt ttgtgtcatt aggcaccctg atattgacta
      241 ttataaagaa ttgattgcta gtccgaacat tcattcagct attgtcaatg ggggtgatgg
      301 ctcaggccaa catccaagcc aatcactttt ggatttggta actatttacg aagaatttgg
      361 atacttcaaa ggactaaaaa ttgcgattgt aggagatcta actcactcac gggtagctaa
      421 atctaacatg caagtgctaa aacgattagg agctgaaatt ttcttttctg gacccaaaga
      481 atggtatagt agtcagttcg atgaatatgg gcaatgttta ccaatagatc agttagttga
      541 tcaaattgat gtccttatgc ttttacgtgt tcaacacgaa cgtcatgatg gtaaaggggt
      601 gttttcaaaa gaaagctatc atcaacaatt tggtcttaca aaagaacgct ataaacattt
      661 aagggataca gcaattatta tgcacccagc acccgtcaat agggatgtag agattgctag
      721 cgatttagta gaagcagata aggctcgaat agtgaaacag atgagtaatg gcgtctatgc
      781 aaggatagct atcttagaag cagtgctcaa tagcagataa taaaggagat aaaatgaagc
      841 gtctattatt attggaagat ggtagtgtgt ttgagggtga ggctttcgga gcggatgtag
      901 gaacaagtgg tgaaattgtt tttagtacag gaatgacagg gtatcaagaa tctataacag
      961 atcaatctta caacggtcaa atcattactt ttacttatcc tcttattgga aattatggga
     1021 taaatcgaga cgattacgaa tctattagac caacatgtaa gggagtaatt gtttatgaat
     1081 gggcagagta tccaagtaac tggcgtcaac agatgacatt tgatgaattt ttaaaattaa
     1141 aaggaatacc tggaatttca ggtatagata ctcgagcatt aacaaaaatt attcgaaaac
     1201 atggaacaat gaaagcttgt ttaataaacg agggaaattc tattcatgaa gcactagaaa
     1261 accttcaaaa aagtgttctt ttaaataatc aaattgaaca agtatcaacc aaattagctt
     1321 atgcttcgcc tggagttggc aaaaatattg tattagttga ctttggttta aaacattcta
     1381 ttctaagaga actgtcacaa cgtcaatgtc atattacagt cgtccctcat actacgacag
     1441 cgcaagaaat cttaaatctc aatccagatg gagtactctt atctaacggc cctgggaacc
     1501 cagaacaatt accgaatgct ttacaaatga tccaagaaat tcaaggtaaa attccaattt
     1561 ttggtatttg tatgggacat caactatttg ctaaagccaa tggtgcaaag acttataaaa
     1621 tgacttttgg tcatcgaggt tttaaccatg ctgttcgtca tttgcaaaca ggacaggttg
     1681 attttacaag tcaaaatcat ggttatgctg tctcgagaga agatttccct gaggctctct
     1741 tcattacaca tgaagagatt aatgataaaa ccgtcgaagg tgttcgacat aaatactatc
     1801 ctgccttttc agtgcagttt cacccagatg cagcaccagg gccgcatgat acatcttatc
     1861 tttttgatga atttattaat atgattgatg atttccagca aaaaagctag ctttaaaata
     1921 tagaattaga ggagtttgat atgccaaagc gcacagacat acgtaaaatt atggttatcg
     1981 gttctggccc gattgtgatt ggacaagcag cagagtttga ctattctggt acacaagcct
     2041 gtctctcttt aaaagaggag ggataccaag ttgttttggt caattcgaat ccggctacta
     2101 tcatgacaga taaagatatt gctgataaag tttatattga acctattacc ttggagtttg
     2161 tcacaagaat tctcaggaag gaaagacctg atgctctcct tccaactctt ggaggtcaaa
     2221 ctggattgaa tatggctatg gctctatcaa aaaatggtat cctagaagaa ttgaatgttg
     2281 aactattagg gaccaaatta tcagccattg ataaagctga agatcgtgat ttatttaaac
     2341 agttgatgga agaactcaat caacctattc cagaatccga gattgttaat tcggtagaag
     2401 aagctattca atttgcagag caaatcgggt atccattaat tgttcgtcct gcctttactt
     2461 taggaggtac aggtggaggc atgtgcgata atcaagaaca attggttgac atcacgacga
     2521 aggggttaaa gttatcgcct gtgacacagt gccttattga acgttcaatt gctggcttta
     2581 aagagattga atacgaagtt atgcgtgatg cagcagataa cgctcttgtt gtttgtaata
     2641 tggagaattt tgatcctgtt ggtatccata caggggactc tatagttttt gcacccgcac
     2701 aaacgttatc tgatgtggaa aatcagttat tacgagatgc gagtttagat attataagag
     2761 ctttgaaaat tgaaggagga tgcaatgttc aactagccct tgatccgaat agttttaaat
     2821 actatgtcat tgaagttaat ccaagagtat caagatcttc tgctttagct tcaaaagcta
     2881 ctggatatcc aattgctaaa ttagcagcta agatagctgt tggtttgaca ctagatgagg
     2941 taattaatcc tattacaaaa acgacttacg ctatgtttga acctgcttta gattacgtag
     3001 tcgcaaaaat gccgagattt ccttttgata aatttgaaag tggcgataga aaattgggaa
     3061 cacagatgaa agctacaggc gaagtcatgg caataggacg gaatatagaa gaatctttgt
     3121 tgaaagcttg tcgttcacta gaaataggtg tcgatcacat taaaatagca gatttagata
     3181 atgtatcaga tgatgtttta ttagaaaaaa taagaaaggc agaagatgat cgcttatttt
     3241 atctagcaga agctttacgt agacattata gtattgaaaa attagcaagt ttgactagca
     3301 ttgattcttt cttccttgat aagttaagag tgattgtaga gttagaagat ctattatcta
     3361 aaaacagact ggatatcaat attctcaaaa aggttaaaaa taaaggtttt tcagataaag
     3421 ctatagcgag tttatggcaa ataaacgaag atcaagttcg taacatgcga aaagaagcag
     3481 gaattcttcc agtttataag atggttgata catgtgcggc tgagtttgat tcagcaacgc
     3541 cctactttta ttcgacttat gccgtagaaa acgagtcatt gatatcagat aaagcctcta
     3601 ttttggtttt aggatcgggc ccaatccgaa ttggacaggg agttgaattt gattattcaa
     3661 cggttcattc tgttaaagcc atcagagagt ctggttttga agcgattatc atgaactcta
     3721 atccagagac ggtctcaacg gatttctcta tttcagataa gctttacttt gaacctttga
     3781 cttttgaaga tgttatgaat gttattgact tagaaaaacc tgaaggggtc atcttacaat
     3841 tcggtggtca gactgctatt aatctagcaa aagatttaaa caaggcaggg gttaaaatac
     3901 taggcacaca attagaagat ttggatcgtg ctgaaaatag aaaacaattt gaagccactt
     3961 tacaagctct aaatatccct caaccaccag gttttaccgc cactacggag gaagaggcag
     4021 ttaatgcagc gcaaaagatt ggttatcccg tgttggttag accatcatat gttcttggtg
     4081 gacgagctat gaagatagtt gaaaacgaag aagatttacg tcattatatg acaaccgcgg
     4141 taaaggcaag cccagatcat ccagtgctta tagatgcata ccttataggt aaagagtgtg
     4201 aggttgatgc tatatccgat ggtcagaata tactgattcc tggaataatg gaacatatag
     4261 agcgagctgg tgttcattca ggagattcca tggctgtata ccctcctcaa acattgtctg
     4321 agaccattat cgaaactatt gtagattata caaaacgctt agcaattgga ttaaactgta
     4381 tcggtatgat gaatattcag tttgtaatca aggatcagaa agtatatgtt attgaagtta
     4441 atcctcgtgc tggtagaaca cttccattct tatcaaaagt aactcacatt cctatggccc
     4501 aagtagcaac aaaagttatc ttaggagata aactctgcaa ttttacatat ggttatgatc
     4561 tctatccagc ttcagatatg gtccacatta aagcccctgt ctttagtttt accaaacttg
     4621 ctaaagtaga tagtttatta ggtcctgaaa tgaaatcaac aggcgaagtg atgggatcag
     4681 atattaatct tcagaaagcc ttgtataaag cctttgaagc agcatatctc catatgccag
     4741 actatggtaa cattgttttt acagtagatg atacagacaa agaagaagct ttagaacttg
     4801 caaaagtcta tcaaagtatt ggttatcgta tttatgctac ccaagggact gcaatttatt
     4861 ttgatgctaa tggtttagag acagttttag taggtaagtt aggagagaat gatcgaaatc
     4921 atattcctga tttaattaaa aatggtaaga ttcaagcagt tatcaacaca gttgggcaaa
     4981 ataacattga taaccatgat gctctcatca tcagacgttc tgcaattgaa caaggagttc
     5041 ctctatttac atctttagat actgcacatg caatgttcaa agttcttgaa agcaaagcat
     5101 ttacattaaa agtactagat taatcaaaca atcaaggtaa aattaacttc cagcaaaaca
     5161 gaatcggact agattaaata tagtttgttc tgtttttata aaaaagccta attgtgataa
     5221 aatagggaac aaacttaagg agttaattat gttaaaaaca cgaacacata ttaatgaatt
     5281 agaaacaaaa atcgcccttg atttagtaca cagatatcta ggtattcaac aacttgtttt
     5341 ttcaaagtgg agacattctt tagaaactgg ttcatggtct tctattataa caataaatgg
     5401 cgctaatatt atcaattaca ttgttgatga tattagcgac agtgatagta tttcaggtat
     5461 tatttgtcat cctaaaagtt ttcatctcag acctagagaa aaaaaggaac ctattcagtc
     5521 tattgatgag ttagatatca tctggtcttt gaatagagag gaaaatgaat gattccagat
     5581 agtagtttta aagtgctatc agatatggca tataatattg aaaaatccaa agcagaaagt
     5641 aatggacacc ttaaaattat agaaggtagt caaattattg atttccatca aaaacgctct
     5701 tttatggtat tagaggttaa tgataatatc gataatggta tgcaggctat ggcatgtcag
     5761 tctttggatg agagtaatca tattgttata gcatattcag gaacgaacgc ttttaataag
     5821 aaagatatct taacagatat cgattcagtt gtattgggga agaacaataa attgctaact
     5881 tgtccttcaa gtattaccct gagagaggat ggcatttctc tgccttgtaa aaatccaagt
     5941 caaattacaa catctaaact atttgcacaa aaaattaaga tgaactatcc tgatgcaacc
     6001 ctctggttaa caggccatgg taaatgttca gtcaggtaat atctcaaatt accaaaaaaa
     6061 tcttttagat tctgcaacta atttccaaaa tatctttcca gccctcgtta accagtatag
     6121 tagttccatt actgctaacg aatcactgaa aaaatcttta gaagcttctg ataacatgtt
     6181 taatgatttg atgacaaccc agacaaatac tggaaaagat ttatcaagct taatagaaca
     6241 gcgccatcaa gatagcattt cgtatgaagc gttttcggcc tcattactag aaatgaataa
     6301 cgagttatta gagaagcaat tatctgatat tatcacacaa gcacaaaaag accaagaaac
     6361 attatcatca caactcaata gtattatggg tgatgataac gataatgatc ataatcataa
     6421 agaaaactca tcagcttatc taaatgttgc aaggcaaaaa atccaagaac tatctgaagc
     6481 actcaagtca caagataaca tcgctaaaga tcaaagtgaa caactagata aaattgttag
     6541 agagggacta gcaagttact ttgctaagaa taataaagat aatattactt tattagaatt
     6601 attgaagagt cattctacta atgagaagac tttgaaagat tttaaagtta aggtagcaga
     6661 tttcacaaat tctctcattt caagcattcc ctcactaaac ctttcggagt tgcacttaac
     6721 ccaagaagaa aaagccattc agtttacatc ttctgattcg gaaatcatta aaaaagtaag
     6781 caatgaaaga actctgtatt taaatactaa tttattgaat cgtctttttg aagctaggaa
     6841 aaatagagat gaagccaaaa ataaagttaa tcaattaaaa ttgccatcta gttctactcg
     6901 aactggtgag cagatagttt ctgttgaatc aaataatcca gattacaggg tagatacctg
     6961 gacggttaat ggtaagcaaa caagaacctt ggaccccagc caaaacaata atattattat
     7021 caatagcagt tatcaagggg aattatctaa ctctagtatt acaaaaccaa gctatacaat
     7081 cactatcggg aatcagaaac aaattgtaca aaatgatgac ggtgaaattc aacggtctta
     7141 cttggaggca gaagcaacgt atcaacgaac tctacaagaa gtaattgatg catataacac
     7201 cactcacgac ttagtagcta aatactacat tatttctgat ggcgaagaac cgcaaaattt
     7261 atttgatcaa tttttaaatc aaagtgttaa cgatacgatg gttgatctgg tcaaaaatgg
     7321 tagaactaag tatttgatgg atgaaaacac tgctgatgcg caacagaaag ttaaagatgt
     7381 catggaggaa gttgaaaata gccaagatga attggcggat caaatggcaa aagtaactga
     7441 gacaaatgta cgactaacag atacaatcaa aaaacagctt gagactcttc aaacaattaa
     7501 catgaaggtc caaaacatca cacaggatca atctaaagta aacgactcac agaaaacaac
     7561 tgatcaacaa ttatctgatt tgaagaacca gctagatggg ctaatgacct ctgcaactgg
     7621 cgtaaaagat atgtccaaat ctaatagtca agaggcggac caagttaatc aaatattcac
     7681 atcattcaat aaagatgttc aagatgctaa aaattcgggc aacaaacttt cgacggatgc
     7741 aacagattta atggctaact tccaaaaaga attggccaat aacggtgatt tcgtagcttc
     7801 tttctctaaa gtttttaata ctgcttataa aaatggagtg ccaaatgata ttctccttaa
     7861 tttcttatca cgacctgtcg ctgaatcagc gtctgcagtt agggcaactg aaaatactta
     7921 tcgcccattc acttggatac tattattaga agttgttagc ctatttatag catatatctt
     7981 tgcgactcaa aaccttatta agaaattgac agataggtat aatgtcaatc gctggcttca
     8041 aacagacttt ttaaatatca ttgtcatttc aggcctctct cttgtgattg gtttagcact
     8101 gggagttatc tcaagcagaa gtcttcatgt catgcctgaa tatgtaccat cttggttcct
     8161 agttatgact ctgtttagtt ttttattaat tcatagtcag tatttcttta ttaaaaattt
     8221 taaagcagtt ggtatgggtt tagctttgtt tatgattatt agttttgtgt atctatcaaa
     8281 tgccgtaggc acagtagcga ctgttagtgg atttccaaaa ttactaaaag ctattaatcc
     8341 actatctatc cttgaaaatc aattatcatc ttattttgat aatgtaacaa ctggatttat
     8401 tttccttatc ttagtacttc ttgtggatgt tgcttttatt atcatgaata tttttatcac
     8461 cttaaatttt gaagctaaag taaaagagtg aggttgttga tgaagttgaa acgattttta
     8521 gcctgtctac taatattatc ctgtatgagt caaacatcag tattttctga tgggttaaag
     8581 aataatgata ttcattttga tacaaaacgt ttagaatcag attcacaaaa aaattctggt
     8641 tttcaaactg aattaactaa agatttattt aaaaaagaag accttaaaaa attggataaa
     8701 aaccgtcaat ctaaagtctc actc
//
LOCUS       DATRUK010000067         8230 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.067, whole genome shotgun sequence.
ACCESSION   DATRUK010000067 DATRUK010000000
VERSION     DATRUK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.067"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            531..777
                     /locus_tag="VBE34_001676"
                     /pseudo
     CDS             531..777
                     /locus_tag="VBE34_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973012.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            796..1050
                     /locus_tag="VBE34_001677"
     CDS             796..1050
                     /locus_tag="VBE34_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7264377.1"
                     /translation="MFVMRTFGNSLSGPLVVILSSILFSWSHLHGLSVVDFVVYFGMG
                     LIFASLHHYTKSIHYSIGEHIVWNSLSYIFYFLAFLLDLL"
     gene            complement(1387..1581)
                     /locus_tag="VBE34_001678"
     CDS             complement(1387..1581)
                     /locus_tag="VBE34_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264378.1"
                     /translation="MIMSGQRATSTIQSDINIFHHIEAICISKSNSIGLPAIQNHLGQ
                     LIQRERLGLFFSITYSKAPT"
     gene            1580..1774
                     /locus_tag="VBE34_001679"
     CDS             1580..1774
                     /locus_tag="VBE34_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264379.1"
                     /translation="MLFLMIKPDYIRYFKVIDDMHDTMYQQTVFHVQLQAWKYTMKSI
                     DQLVVDDLNHISNEKTTLVK"
     gene            complement(1825..2001)
                     /locus_tag="VBE34_001680"
     CDS             complement(1825..2001)
                     /locus_tag="VBE34_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264380.1"
                     /translation="MSKKTDSNYLHSNCSDKKIWSDEAPLQQETIPKDRDCVIKPVIT
                     MTVEHVEIPLDDQS"
     gene            complement(2037..3626)
                     /locus_tag="VBE34_001681"
     CDS             complement(2037..3626)
                     /locus_tag="VBE34_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HEO7264381.1"
                     /translation="MKIKKIISGFAAALIISSLSTINYEVKADDTTSEYHYISKQNNE
                     KQLISYIKEQHRLLNQFVVDNVNSFTQLNANPTIEQLNRAITLFKQKDEQLFNQVKAG
                     HLSPSNYNAIVNQRNVINQTVQNLIDQNHNKIQTSQNKAAQLVGQRNQVVNKIQAILA
                     TVNYNSVNSIQEAENLFHSLRNQIEPLVAEVNNYKAAMAILQQEVDALSTAAIETETS
                     KLATLKVSENTSVPANKVEEKTTQSEASGNKQEVTKSEEKQATSDAKASQPESANIAD
                     YDSLKEVLRNNISNQVPHISVQMEFKTQEQVDEYQKNLGSIIREIGDTLGTATEFNAK
                     SNISTYTLGGQIQRIIVKSDITITYTLKGDMVGLHKEYKQFVDSFVKENITNKNITSD
                     YEKAKVIHDHLVNNYTYATEELATTRETASGISIHAPEALYKDKRGVCQAFAVMFKDM
                     AAAGLSVWYVTGQAGGGNHAWNIVTINGVKYYVDTTWDNNIKSNKYFLVGKTIMDADH
                     LLDSQYNALAKDIPADRHQGA"
     gene            complement(3687..3755)
                     /gene="shp2"
                     /locus_tag="VBE34_001682"
     CDS             complement(3687..3755)
                     /gene="shp2"
                     /locus_tag="VBE34_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEO7264382.1"
                     /translation="MKKINKALLFTLIMDILIIVGG"
     gene            3843..4691
                     /gene="rgg2"
                     /locus_tag="VBE34_001683"
     CDS             3843..4691
                     /gene="rgg2"
                     /locus_tag="VBE34_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HEO7264383.1"
                     /translation="MEKELGKTLRRLRKGKKVSISSLADEHLSKSQISRFERGESEIT
                     CSRLLNILDKLNITIDEFVSIHSKAHTHFFILLNRVRKYCAEKNVTKLVALLEDHNHK
                     DYEKIMIKALIFSIDQSIEPNQEELARLTDYLFTVEQWGYYEIILLGNCSRLINYNTL
                     FLLTKEMVNSFAYSEQNKTNKILVTHLAINCLIISIDHSYFEHSHYLIDKVRSLLQDE
                     VNFYEKTVFLYVTGYYHLKLGDTSSGKEDMRKALQIFKYLGEDSFYNTYNEHYCQKVL
                     GNNKEC"
     gene            4840..5661
                     /gene="mutM"
                     /locus_tag="VBE34_001684"
     CDS             4840..5661
                     /gene="mutM"
                     /locus_tag="VBE34_001684"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEO7264384.1"
                     /translation="MPELPEVETVRKGLERLVVNQEIASITIKVPKMVKTDLNDFMIS
                     LPGKTIQQVLRRGKYLLFDFGEMVMVSHLRMEGKYLLFPNKVPDNKHFHLYFKLTNGS
                     TLVYQDVRKFGTFELVRKSSLKDYFTQKKLGPEPTADTFQFEPFSKGLANSKKPIKPL
                     LLDQRLVAGLGNIYVDEVLWAAKIHPQRLANQLTESETSLLHKEIIRILTLGIEKGGS
                     TIRTYKNALGEDGTMQKYLQVYGKTGQPCPRCGCLIKKIKVGGRGTHYCPRCQCL"
     gene            5652..6245
                     /locus_tag="VBE34_001685"
     CDS             5652..6245
                     /locus_tag="VBE34_001685"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEO7264385.1"
                     /translation="MLMTKIIGLTGGIASGKSTVTKIIRESGFKVIDADQVVHKLQAK
                     GGKLYQALLEWLGPEILDADGELDRPKLSQMIFANPDNMKTSARLQNSIIRQELACQR
                     DQLKQTEEIFFMDIPLLIEEKYIKWFDEIWLVFVDKEKQLQRLMARNNYSREEAELRL
                     SHQMPLTDKKSFASLIIDNNGDLITLKEQILDALQRL"
     gene            6369..7070
                     /locus_tag="VBE34_001686"
     CDS             6369..7070
                     /locus_tag="VBE34_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7264386.1"
                     /translation="MELVIRDIRKRFQETEVLRGASYRFYSGKITGVLGRNGAGKTTL
                     FNILYGDLAADNGTICLLKDNHEYPLTDKDIGIVYSENYLPEFLTGYEFVKFYMDLHP
                     SDDLMTIDDYLDFMEIGQTERHRIIKGYSDGMKSKLSLICLMISKPKVILLDEPLTAV
                     DVVSSIAIKRLLLELSEDHIIILSTHIMALAEDLCDIVAVLDKGKLQTLDIDRKHEQF
                     EERLLQVLKGDEYDK"
     gene            7295..>8230
                     /locus_tag="VBE34_001687"
     CDS             7295..>8230
                     /locus_tag="VBE34_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001882372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264387.1"
                     /translation="MKQLNILPQEGFTLTLTLMVITSLLIVINFYIKLIEPNDMQQTH
                     MIRLFRIEPSRYFQSAELLEASSELFFRSLSFGIFFHLNHFAFWHGLTFALFLAGSRL
                     GIKVLCLPLYGRGKDQENPESLLNILFYLGIGIGVFLSLFFLLLGKQFSPYPFFSWYT
                     GIAGLIIWLVSYYRLKTYSKLNHLTYLTLTHETMKEKIAKIDNIELLGIEMKDRDINV
                     SELSPLLFENLSGISYLNAIFLKRMSRYLQRKILVRLAVLSVLFGIELLFLLFFKEKI
                     NIPMNEHSFSKFLFLSAVPGYLIYIGESYSKFCFYH"
BASE COUNT         2620 a         1237 c         1495 g         2878 t
ORIGIN      
        1 taggaatgat tttatcatta ggtcgtatca tttctcctga acactctatg tttaataaac
       61 ttagaaagtg aaatgaatta tatttaaaat ttgttttcaa cagagcggat ttgaccatct
      121 gttgtttgcc atgcctttca taatttttca gtaattatga tagttgttct gttctcccaa
      181 tcataaaact ttaaataaga ttctattagt tcttgcctca ctctccttaa gtttctggta
      241 aaaatcgaaa agttaccaga ctttaaaggg gagtttttta tttgtcaaca gttagtagaa
      301 aaacgatata ttgctccatt tagattaatt atggagataa ccaaataata gcttgtgata
      361 agaagatacc tttgaagttt cagaaacaat ctgttttcca tcctgctata ttcatctttg
      421 ccaaagttgc aacaacacaa taataacaat tgcttgggca ttgggtgatt agtttactat
      481 caatagggtt agagtggagt tggagtatat ggattggcat taatcttata atgatgattc
      541 tgttgagtta tgtgctaaca aaaactgagc caatcagtaa ctaccaagag tcatcatcta
      601 acctcaaaaa atggctattt gcaataggtg cttttagcgt tttattttgc tgggcactac
      661 tagtaatttt tcccaataag gcaaaaccag gagggattaa tagctgatat gaatcaggtc
      721 aatcactact ttcgagttgt cttattattg actatctgtc ttattgcccc aatttaagaa
      781 gagataacgt tcaggttgtt tgtcatgaga acatttggga actccctgag tggtcccttg
      841 gttgtgatct tatcttctat actatttagc tggtctcact tacatggtct atctgtcgtc
      901 gactttgttg tctattttgg tatgggtttg attttcgcta gtttgcacca ctatacaaaa
      961 tccattcatt attcgattgg cgagcatatt gtttggaata gtttgtcata tattttttat
     1021 tttctagcat ttttgcttga tttactctaa gtaataagat tgagagacta atcattacta
     1081 agacattttt gtcagtttaa aagagctttt tggctctttt gttattcgtt tttttaatga
     1141 ataaaatgtt attattttat tgttaactaa ataaagttcg taactttata tgatttatat
     1201 agaattacga gttttttgtt agataaatat gagaaaatat agtatgacga attcctaaat
     1261 aaaaaataat gaaaggaaca tgatgttaaa agatgtgaat gttggaatga gtcatttatt
     1321 agttagaggt gctattattc tagtagtagg gattgttttt ctgttgcaac agattcagta
     1381 agtacttcaa gtgggagctt tggaataggt tatcgagaag aaaagaccca gacgttctct
     1441 ttggatcagc tgtcccaagt gattttgtat tgcggggagc ccaatactgt tagatttact
     1501 tatacaaata gcttctatat gatgaaagat atttatatcg ctctggatgg tgctggtagc
     1561 tctttgacca ctcatgatca tgctgttcct aatgattaaa ccagattata taaggtattt
     1621 caaagtcatt gacgatatgc atgataccat gtaccaacag actgtttttc atgttcaact
     1681 gcaagcttgg aaatacacca tgaaatcgat tgatcaatta gttgtagatg atttgaatca
     1741 tatcagtaat gagaaaacca ctttggttaa ataaaaagga acactgtcaa aaatggcagt
     1801 gttccttttt attttaggag agagctaaga ttggtcatct aatgggattt ccacatgttc
     1861 aactgtcata gtaatgacag gtttgataac acaatctctg tctttaggga tagtctcctg
     1921 ttgcagaggc gcctcatcgc tccaaatttt cttgtcagaa caattggaat gtagataatt
     1981 tgaatcagtc tttttagaca tttcatatcc tttccaggct caatacaatt tttgagttat
     2041 gcaccttgat ggcgatcagc tggaatatct ttagctaatg cattgtattg actatccaaa
     2101 agatgatcag catccattat tgttttacca acaaggaaat atttattgct ttttatatta
     2161 ttatcccatg ttgtatcaac ataatattta acgccattaa tagtaacaat gttccaagcg
     2221 tgatttccac ctccagcttg accagttaca taccatactg ataagccagc agcagccata
     2281 tctttaaaca ttactgcaaa ggcttgacaa acaccacgtt tatctttgta gagtgcttca
     2341 ggagcatgga tactgatacc actagcagtt tcacgagtgg ttgccagttc ttcagtcgcg
     2401 taagtgtaat tattaaccaa gtggtcatga attactttag ctttttcata atcacttgtg
     2461 atatttttgt tagtaatatt ttctttgaca aaagaatcta caaactgttt atattcttta
     2521 tgtaatccta ccatgtcacc tttaagagta taggtgattg tgatgtcgct ttttacaata
     2581 atgcgttgga tttgtccacc aagagtataa gtgctaatgt tacttttggc attgaattca
     2641 gttgctgttc caagtgtatc tccaatttcc cggatgatgc ttccgagatt tttttggtat
     2701 tcgtcaactt gttcttgagt tttaaactcc atttgaacac tgatgtgtgg tacttggttg
     2761 ctaatattat ttcgtaaaac ttcttttaaa ctatcgtaat cggcaatatt agctgactca
     2821 ggctgtgatg cctttgcatc agaggtagcc tgtttttcct cactcttagt tacttcttgt
     2881 ttattgcctg acgcttctga ttgagtagtt ttttcttcta ctttgtttgc aggaacagaa
     2941 gtattttcgc taactttgag agtagcaagt ttagaagtct cagtttcaat agccgctgtt
     3001 gatagggcat ctacttcttg ttgaaggatt gccatagcag ctttgtaatt attaacttca
     3061 gctacaagag gttcaatttg atttctgagt gaatgaaata aattttcagc ttcttgtata
     3121 gaattcacag agttgtagtt tacagttgct aaaatagctt gaattttgtt aacaacctga
     3181 ttacgttgcc ccacgagctg agctgcttta ttttgacttg tctgaatctt attatgattt
     3241 tggtcaatca gattttgaac agtttggtta atgacattac gttgattaac gatagcatta
     3301 tagttactgg gagagagatg accagctttc acctggttaa ataattgctc atctttttgt
     3361 ttaaataatg ttatagctct atttaactgt tcaatagttg gattagcatt tagttgagtg
     3421 aatgagttga cattatcaac aacaaattga ttgagcaaac gatgttgttc cttgatgtaa
     3481 ctaataagct gcttttcatt attttgctta ctgatatagt gatactcact ggtggtgtca
     3541 tcagctttaa cctcatagtt aatagttgat agtgaactga taattaaagc tgcggcaaag
     3601 ccactaataa tttttttaat cttcattagt tctcctgtta taaactaaat aaaatgggat
     3661 tccataacaa ccatttgtgg ggagttttat ccaccaacga taattaggat atccatgatt
     3721 aatgtgaaca acaaagcttt gttaattttt ttcatgacta tctcctttct gattttctat
     3781 tttgtacttg tattatataa tggatttaaa gggtctttta aattgttgtg aaagtgggaa
     3841 aaatggaaaa agaattagga aaaacactaa gaagattacg taaaggaaaa aaagttagta
     3901 tttcttcttt agcagatgag cacctatcca agtcacaaat ctcaaggttt gagagaggag
     3961 agtcagaaat tacttgtagt cgtttgctta atattttaga caaactcaac atcactattg
     4021 atgaatttgt gtcgattcat tctaaggcgc acactcattt ttttatcctt ttaaatcgag
     4081 taagaaaata ttgtgctgaa aaaaatgtaa ctaaattagt ggctctctta gaggatcata
     4141 accataaaga ctatgaaaag attatgatta aggctcttat tttttcaatt gatcaatcaa
     4201 tagagcctaa tcaagaagaa ttagcacgct taacagacta tctttttacg gtggagcagt
     4261 ggggatatta tgaaattatt ctcttaggaa attgctcacg tctcattaat tacaatactt
     4321 tatttttatt gaccaaagaa atggttaatt cgtttgctta ttcagaacaa aataaaacca
     4381 ataaaatcct agttacacac ttagcaatta attgtctaat catcagcatt gatcactctt
     4441 actttgagca tagccactac ttaattgata aggttagatc attgttacag gatgaagtta
     4501 atttctatga aaaaactgtt ttcttatatg taactggata ctatcattta aaacttggtg
     4561 atacttcttc aggaaaagaa gatatgagga aagctttaca gatttttaaa tatttaggtg
     4621 aagattcatt ttacaatact tataatgaac attattgtca aaaagtactt ggcaataata
     4681 aagaatgcta atgagtgcaa gtttatgagt aatttttaaa gcttttgtct caaaatgata
     4741 ataagaaagg aaaatagaaa tcgaatggtc aggtgacatc aagaacgttt ggaaattatt
     4801 gctctaagca atgactattg atttttagac tatatattta tgccagaatt accagaagta
     4861 gaaaccgtta gaaaaggact agaaagactt gtagttaatc aagaaatagc ttctataacg
     4921 attaaagtcc ctaaaatggt gaaaactgat ttgaatgact ttatgataag tctaccagga
     4981 aaaacaattc aacaggtatt gagacgaggg aagtatcttc tttttgattt tggtgaaatg
     5041 gttatggtat cacatttacg catggaagga aaatatctac tcttcccaaa taaagtacca
     5101 gataacaaac attttcacct ttatttcaaa ttgactaatg gcagcacatt ggtttaccaa
     5161 gatgtccgta aatttgggac atttgagttg gttcgtaaat catctctcaa agattatttt
     5221 actcaaaaaa aattaggtcc tgagccaact gctgacacat ttcaatttga gcctttttca
     5281 aaaggcttag caaactccaa aaagccaata aaacctttac tattagatca gagattagta
     5341 gcaggacttg gaaatattta cgtagatgaa gtcttgtggg cagcaaaaat tcacccacag
     5401 cgtttggcta atcaattgac cgaatctgaa acaagcttat tacacaagga aattattcgc
     5461 attttaactt taggcatcga aaaaggtggc tccacgatta gaacatataa gaatgctctt
     5521 ggagaggacg gtacgatgca aaagtattta caggtctatg gtaaaactgg gcaaccgtgt
     5581 ccaaggtgtg gttgtttaat taagaagata aaagtaggag ggagagggac gcattattgc
     5641 cctcggtgtc aatgcttatg acaaaaataa taggactgac aggagggata gcttctggaa
     5701 agtcaacggt aacaaaaata atacgagaat caggttttaa agtcatagat gcggatcaag
     5761 tggttcataa attgcaagct aagggtggga aactttacca agctttatta gaatggttgg
     5821 gtcccgagat acttgatgct gatggtgagt tggatagacc aaagctttct caaatgattt
     5881 ttgctaatcc agacaatatg aagacatcag ctaggctaca aaatagtatc attcgtcaag
     5941 agttagcatg tcagcgcgac caattaaaac aaacagaaga gatatttttc atggatattc
     6001 ctttattgat tgaagaaaag tatataaaat ggtttgatga gatttggttg gtatttgttg
     6061 ataaagaaaa acaattacaa cgattaatgg cccgtaacaa ctacagtcga gaagaagcag
     6121 aattacgact ttcacaccaa atgcctttaa cagataaaaa aagtttcgct agtcttatta
     6181 ttgacaataa tggtgattta ataactttaa aagagcaaat attggatgct cttcaacgtt
     6241 tatagaacta ctaaatggat aatgtattta tccatttttt gtctttatta cactagtttt
     6301 aaaaaacttg cgattaaacc acagtaatag tacaatgata taaaaggttt actttcaaag
     6361 gagtttttat ggagttagta attagagata ttcgtaagcg gtttcaggaa acagaggtct
     6421 tgagaggagc aagttaccga ttttattcag gtaaaataac aggggtctta ggtaggaatg
     6481 gtgctgggaa aacaacttta tttaatatac tttatgggga tcttgcagct gacaacggga
     6541 ccatttgttt attgaaggat aatcacgagt atcctcttac cgataaggat attggtattg
     6601 tttattccga aaactacctt ccagaatttt taacagggta tgaatttgta aaattttaca
     6661 tggatttaca tccttcagat gatttaatga caatagatga ttatttagat tttatggaaa
     6721 taggacaaac agagcgtcat agaattatca aaggatattc tgatggaatg aagagtaagc
     6781 tctcattaat ttgcctgatg atttctaagc caaaagtaat tttactagat gagccactga
     6841 ctgcagttga tgttgtatca agtattgcaa taaaacgcct tttgttggaa ttaagtgagg
     6901 atcatattat tatattatca actcatataa tggccttagc agaagatcta tgtgatattg
     6961 tggctgtatt agacaaagga aaactccaaa cattagatat tgatcgtaaa catgaacaat
     7021 tcgaagagcg tcttcttcaa gtgttgaagg gagatgaata tgacaagtaa agcattaata
     7081 tggtctatat tacagattga tagtgctaga gcttataata gttttttata ttaccttcag
     7141 aaaatcccct ggttgggaag aaaaatccct aaccgatggt ataaaaggtg tagtactatt
     7201 tattcgtatc attttctttt tcttaagagg ttttggtcgt tccataggtt acgtactata
     7261 ttgttatgtt ttatcatttt ttgttagaag tggcatgaag cagttaaata ttttaccgca
     7321 agaaggtttt accctaactt taacattgat ggtgattacc tctcttttaa ttgtcataaa
     7381 tttctatata aaacttatag aacctaatga tatgcaacaa actcatatga ttcgcctttt
     7441 cagaattgag ccatcgaggt atttccaatc cgctgaactt ttagaagcca gtagtgaact
     7501 attttttaga agtttgagct ttggcatatt tttccattta aatcactttg ctttttggca
     7561 tggtttgact tttgctttat ttttagcagg tagtcgcctt ggaatcaagg tgctctgttt
     7621 acctttatat ggtagaggta aagaccaaga gaatccagag tctttactaa atattttatt
     7681 ttacttaggg attggtatag gagtcttcct ttctctcttt ttccttcttc ttggcaaaca
     7741 gttctctcca tatccatttt tttcatggta caccggtatt gcaggactta tcatatggtt
     7801 agttagttac tatcgattaa aaacttattc taaacttaac cacttgacct accttaccct
     7861 aacgcatgag acgatgaagg agaaaattgc aaagattgat aatatagagc tgcttgggat
     7921 tgaaatgaaa gatagggata taaatgtcag tgaactttct cccctgctat ttgaaaattt
     7981 atcaggtatt tcatatttaa acgctatctt tttaaaacgg atgagtagat acttgcaaag
     8041 gaaaattttg gtacgtttag ctgttttaag tgttctgttt ggaatagaac tccttttcct
     8101 gctatttttt aaagaaaaga taaatattcc gatgaatgag catagttttt caaaattcct
     8161 atttttgagt gcagttccag gatatctaat atatatcggt gaatcctatt caaaattttg
     8221 tttctatcat
//
LOCUS       DATRUK010000068         8220 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.068, whole genome shotgun sequence.
ACCESSION   DATRUK010000068 DATRUK010000000
VERSION     DATRUK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.068"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..146)
                     /locus_tag="VBE34_001688"
     CDS             complement(<1..146)
                     /locus_tag="VBE34_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7264388.1"
                     /translation="MTKHKHLTLSDRNDIQSGLDRGETFKAIGLNLLKHPTTIAKEVK
                     RNKQL"
     gene            413..1747
                     /locus_tag="VBE34_001689"
     CDS             413..1747
                     /locus_tag="VBE34_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEO7264389.1"
                     /translation="MEDPGSQSLLLQFVILLILTLFNAFFSASEMALVSLNRSKVEQK
                     AEEGDKRYRRLLDVLENPNNFLSTIQVGITFISLLQGASLSASLGHVISGWLGNSATA
                     RTAGSIIALIFLTYVSIVLGELYPKRIAMNLKDRLAIVSAPIIIFLGKIVSPFVWLLS
                     ASTNLLSRITPMTFDDADEKMTRDEIEYMLTNSEETLDAEEIEMLQGIFSLDEMMARE
                     VMVPRTDAFMIDINNDAQSNIEGILSQNFSRVPVYDDDKDRVVGVLHTKRLLEAGFKT
                     GFDTIDLRKILQEPLFVPETIFVDDLLKALRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDTAEQFVREIDENIYIVLGTMTLNEFNDYFETELESDDVDTIAGYYLT
                     GVGSIPNQEEKVAYEVDSKDKRITLINDKVKDGRITKLKVLLSDIEQNIEKD"
     gene            1815..2603
                     /gene="pflA"
                     /locus_tag="VBE34_001690"
     CDS             1815..2603
                     /gene="pflA"
                     /locus_tag="VBE34_001690"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEO7264390.1"
                     /translation="MAEIDYKKVTGMIHSTESFGSVDGPGIRFIIFMQGCKMRCQYCH
                     NPDTWEMETNNSKERTVEDVLKEALRYKHFWGKDGGITVSGGEAMLRIDFITALFIEA
                     KKLGIHTTLDTCGFAYRATPEYHAILEKLLDVTDLVLLDLKEIDPEQHKIVTRQSNKN
                     ILQFARYLSDRGTPVWIRHVLVPGLTDIDDHLKRLGEFVQTLDNVDKFEVLPYHTMGE
                     FKWRELGIPYPLAGVKPPTPERVKNAKDIMKTESYTEYLKRIQN"
     gene            2720..3655
                     /locus_tag="VBE34_001691"
     CDS             2720..3655
                     /locus_tag="VBE34_001691"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEO7264391.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAWGLDTEAVALGTPN
                     EETAYVLDYFGVQAPRVVESAKAEGVETVILTDHNEFQQSISDIKDVTVYGVVDHHRV
                     ANFETANPLYMRLEPVGSASSIVYRMFKENGVSVPKELAGLLLSGLISDTLLLKSPTT
                     HASDIPVAKELAELAGVNLEEYGLEMLKAGTNLSSKTAAELIDIDAKTFELNGEAVRV
                     AQVNTVDINDILARQEEIEVAIQEAIVTEGYSDFVLMITDIVNSNSEILALGSNMAKV
                     EAAFEFTLENNHAFLAGAVSRKKQVVPQLTESYNA"
     gene            3715..4368
                     /locus_tag="VBE34_001692"
     CDS             3715..4368
                     /locus_tag="VBE34_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEO7264392.1"
                     /translation="MILTFNPGKLERQEFFKELINYLWIHDDVTLRQIKSHFTDYSKI
                     DRLLEEYINHGYILRQNKRYSLNLPFLSSLDGLALDDLVFIDSASQIYQLLQKRKFVT
                     NLDNQTNHLVFVEETDFERNTLTLSNYFYKLTNCYPLSREQKKLYQLLGDVNSEYALK
                     YMSSFILKFLRKDSVKQKRTDIFIQALELLGYISLNQDTTYRLNAKLDVEALKIYLT"
     gene            complement(4398..5399)
                     /locus_tag="VBE34_001693"
     CDS             complement(4398..5399)
                     /locus_tag="VBE34_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO7264393.1"
                     /translation="MIQKNKAPFVLISSVIILLLLILVSISLGYANTSVIDVLKLISG
                     KSDDAFLFIITNIRLPRIIVCIFGGASLGIAGLLLQTLTKNPLADSGILGINAGAGLV
                     IALTIGTFNVSNPTILYFLPLFAMFGGLVTIFLVYLMSYRRNHNISPTRLIITGIGIS
                     TMISGVMISIISQSNNQKMDMIVEWLSGKITISSWTTIITFIPILILLWGLAYSRSRH
                     LNIMNLNEQTALALGLHLKKERIYTLMLASSLAAISVVLIGNITFIGLLAGHLSRRLL
                     GNNHKIILPSCLLIGAIILLVSDTIGRLLLVGTGIPTGLVVSIIGAPYFLWLMTKVD"
     gene            complement(5396..6421)
                     /locus_tag="VBE34_001694"
     CDS             complement(5396..6421)
                     /locus_tag="VBE34_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO7264394.1"
                     /translation="MFKASKKLVQKNKSNHFWLVFFITLILFLIGCYASLRFGAINFK
                     TSDLITVLKNPLKNSNAQDVIFDIRLPRIIAAILVGAAMSQAGAIMQGVTRNAIADPG
                     LLGINAGAGLALVVAYAFLGSMHYSTILIVCLLGSVISCLLVFTLSYTKQKGYHQLRL
                     ILAGAMISTLFTSVGQVVTLYFKLNRTVIGWQAGGLSQINWKMLIIIAPIIILGLLIS
                     QLLAHQLTILSLNESVAKALGQKTQLMTAFLLLIVLFLSASSVALIGTVSFIGLIIPH
                     FIKLFIPKDYRLLLPLIGFSGATFMIWVDLSSRIINPPSETPISSIISIVGLPCFLWL
                     IRKGKNL"
     gene            complement(6437..7369)
                     /locus_tag="VBE34_001695"
     CDS             complement(6437..7369)
                     /locus_tag="VBE34_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7264395.1"
                     /translation="MKKIGIIVLTLLTFFLVSCRQQTKEESTKTTISKMPKIEGFTYY
                     GKIPENPKKVINFTYSYTGYLLKLGVNVSSYSLDLEKDSPVFGKQLKEAKKLTADDTE
                     AIAAQKPDLIMVFDQDPNINTLKKIAPTLVIKYGAQNYLDMMPALGKVFGKEKEANQW
                     VSQWKTKTLAAKKDLHHILKPNTTFTIMDFYDKNIYLYGNNFGRGGELIYDSLGYAAP
                     EKVKKDVFKKGWFTVSQEAIGDYVGDYALVNINKTTKKAASSLKESDVWKNLPAVKKG
                     HIIESNYDVFYFSDPLSLEAQLKSFTKAIKENTN"
     gene            complement(7393..8187)
                     /locus_tag="VBE34_001696"
     CDS             complement(7393..8187)
                     /locus_tag="VBE34_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7264396.1"
                     /translation="MSHIKSENIIVSYDQKEIINNLSLSILNQKITTIIGANGCGKST
                     LLKALTRIHKIKDGTITIDGHDIAHLPTKEIAKKIALLPQVLEATEGITVYELISYGR
                     FPHQKYLGNLTNDDRSKIHWAMEMTNVAQFANRDVDDLSGGQRQKVWIAMALAQDTDT
                     IFLDEPTTYLDMNHQLEVLELLKKLNNETQKTIIMVLHDLNLSARYSDYLVAMKTGKI
                     IYEGSPSQIMTKDIIKDIFKIDAHIIQDPISKQPVLLSYQLSTDQN"
BASE COUNT         2698 a         1307 c         1530 g         2685 t
ORIGIN      
        1 agttgcttat ttcgtttgac ttcttttgcg atagtagtag ggtgtttcag tagattaagc
       61 ccgatggctt taaaggtttc tcctctatcc aaaccagatt ggatgtcatt acggtctgag
      121 agagttaggt gtttgtgttt tgtcatgatg gataaactca tttctaaccc aaacgtctca
      181 gacttaattc caccataaaa atccttttca cactaacttc cggttttgga gggcagacgg
      241 aacttacttt gagaaattac taattgctat aaaatgccat aaacaatatt atcgactatg
      301 cgctcagtgc tattatatga tataataata gcattatcta atagaagaac agttggtaga
      361 gttaatcgct atcagctaaa ttcaaataat ttttatttag gagtaatttt ccatggaaga
      421 ccctggcagt cagagtttgt tactacaatt tgttatttta ttaatattaa ccctgtttaa
      481 cgccttcttt tcagcatctg aaatggcttt agtatcgcta aaccgctcta aagtagaaca
      541 gaaagctgaa gaaggggaca aaaggtatcg acgtcttttg gacgttcttg aaaatcccaa
      601 taatttttta tcaacaattc aagttggtat tacttttatt agccttttgc aaggggctag
      661 tttatctgcc tcactaggac atgtgatttc aggatggcta ggaaattcag caacagcccg
      721 tacagcaggg agtattattg cgctaatctt tttaacatat gtctctattg ttctaggaga
      781 attatatcct aagcgtattg ctatgaattt gaaagatcgt ctagcgattg tttcagctcc
      841 cattattatc tttctgggga aaatagtaag cccctttgta tggttgctct cagcttcaac
      901 taacttattg agtagaatca ctccgatgac ttttgatgat gctgacgaga agatgacacg
      961 tgatgaaatt gaatatatgt tgacgaatag tgaagaaact ttggatgctg aagaaattga
     1021 gatgctgcaa gggattttct cgctagatga aatgatggcg cgtgaagtta tggttccgcg
     1081 tactgatgct ttcatgattg acatcaacaa tgatgcacaa tcaaatattg aaggaatttt
     1141 atcacaaaac ttttctcgtg tccctgttta tgatgacgat aaagatagag ttgttggtgt
     1201 tttacatacc aagcgcttat tggaagcggg ctttaaaact ggttttgata ctatcgactt
     1261 gcgaaaaata cttcaggaac ctctttttgt tccagaaacg atttttgtag atgatctttt
     1321 aaaagcatta cgcaacactc aaaatcaaat ggccatttta ctagatgaat atggtggcgt
     1381 tgctggttta gtaactctag aggatttatt ggaagaaatt gtcggcgaaa ttgatgatga
     1441 aacagataca gctgaacaat ttgtcaggga aatcgatgag aatatctata ttgttctagg
     1501 aactatgacg cttaatgaat ttaatgatta ttttgagaca gagttagaaa gtgatgatgt
     1561 tgatacaatt gcgggttatt atttaactgg tgtgggtagt attccaaacc aagaggaaaa
     1621 agtagcttac gaagtagaca gcaaagataa acgcatcact ttgattaatg ataaagttaa
     1681 agatggtcgt ataacaaaat tgaaagtgct attgtctgat atagaacaga atattgaaaa
     1741 agactaagat tacttagtct ttttttcatg aaaatgatat aataaagtca taaaaacggt
     1801 tacaaggtga tgaaatggca gaaatagact ataaaaaagt aacaggaatg atccattcta
     1861 cagaaagttt cggctctgtg gatgggccag gtattcgttt tatcattttt atgcaaggat
     1921 gtaaaatgcg ttgtcaatac tgtcataatc ctgatacttg ggaaatggaa actaacaatt
     1981 caaaggaacg caccgttgag gatgttctaa aagaagctct tcgttataaa catttttggg
     2041 gaaaagatgg aggtatcaca gtttcaggtg gtgaagcaat gctgcgaatt gactttatta
     2101 cagctctctt tattgaggct aagaaactag gtattcatac gacgctagat acttgtggtt
     2161 ttgcttatcg agcaacacca gaataccatg ctatccttga aaaattgtta gatgttactg
     2221 atttggtact ccttgactta aaagaaattg atccagaaca acataaaatt gtaactcgtc
     2281 agtctaacaa aaatatttta cagtttgcta gatatttatc tgatagaggt actccggtat
     2341 ggattcgtca tgttttagtt cctggattga ctgatattga tgaccattta aaacgtttgg
     2401 gtgaatttgt ccaaacactt gataatgtcg ataaatttga agtattgcct tatcatacta
     2461 tgggagagtt caaatggcgt gaattaggaa ttccgtatcc attagctggt gtgaaaccac
     2521 caactccaga acgtgttaaa aacgctaaag atatcatgaa aacagaatcc tatacagaat
     2581 atctaaaaag aattcaaaat tagttttaag ttgaatttta ttcaactttt ttagttgcaa
     2641 agggcttcct agtaagattt tttgctaagt tattcaattt ttgctacaat aaggtgaata
     2701 taaaagaaga ggtaaaaaca tgtctaaaat tttagtcttt ggtcaccaaa atccagattc
     2761 agatgctatt ggatcatcag ttgcttttgc atatctagct aaagaagctt gggggcttga
     2821 cacagaggcc gttgctttag gaactccaaa tgaagaaaca gcatatgttt tagattattt
     2881 tggtgttcaa gcacctcgag tagtagaatc tgcaaaagca gagggtgtag agacagtcat
     2941 tttaactgac cataacgaat tccaacaatc tatttctgat attaaagatg tgacagttta
     3001 tggtgttgtt gatcatcatc gtgttgctaa ctttgagact gccaatccgt tatatatgcg
     3061 tttggaacca gttggctcag catcatcgat tgtttaccgt atgtttaagg aaaatggagt
     3121 atcagtaccc aaagagttgg ctggtttatt attatcaggt ttgatttcag atactcttct
     3181 tttgaaatca ccaacaactc atgcttcaga tattccagtt gcaaaagaac tagcagagct
     3241 tgcaggtgta aacttagaag aatatggttt agaaatgctt aaagcaggta ctaacctctc
     3301 aagtaagact gcagcggagt taattgatat tgatgctaaa acatttgaat taaatggaga
     3361 agctgttcgt gttgcacaag ttaatactgt ggacattaac gatattttag cacgtcaaga
     3421 agagattgaa gttgctattc aagaagcaat tgtcacagaa ggttattcgg attttgtatt
     3481 gatgattact gacattgtta attcaaattc agagatttta gctctcggtt caaatatggc
     3541 taaagttgaa gcagcttttg aatttacact tgaaaataac cacgcttttc ttgctggggc
     3601 agtatcacgt aaaaaacaag ttgttcctca attgacagaa agctacaatg cttaattaat
     3661 catgtaaaac gggctgcgtc ccgttttttt agtcatacat ttaggagcgt ttttatgata
     3721 ttaacgttta atccaggaaa gttagaaaga caagagtttt ttaaggagct tattaactat
     3781 ctttggatac atgatgatgt aacactaaga caaataaagt cccactttac agactattct
     3841 aaaattgacc gtttgttaga agaatatatt aatcatggat atatccttcg ccaaaataaa
     3901 cgctacagcc ttaatcttcc ttttctgagt tcgctggatg gattagcatt agatgatttg
     3961 gttttcatag attctgctag tcaaatctat cagttattac agaagcgaaa atttgtgaca
     4021 aatttagata accaaaccaa tcacttggta tttgtagaag agacagattt tgaaagaaac
     4081 actttgactc tttctaatta tttctataaa ctaactaact gttatccact ttcaagagaa
     4141 caaaaaaagc tttatcaact tttaggagat gttaattcgg aatatgcttt aaaatatatg
     4201 tcaagcttta tattgaaatt tcttcgcaag gatagtgtta agcaaaaacg tacagatatt
     4261 tttatccaag ctttagaatt acttggatat attagcttaa accaagatac tacctatcgt
     4321 ttaaatgcta aacttgatgt tgaagcttta aagatttact taacataaaa aaagactgga
     4381 agtttgtcca gtctttttta gtcaactttt gtcatcaacc agagaaaata gggggcacca
     4441 attattgata caaccaatcc agtgggtata cctgtaccaa ctaagaggag acgacctata
     4501 gtatctgata ccaataggat aatagcacca ataagaagac agctaggtag tataatttta
     4561 tggttatttc ctaatagtcg tctacttaag tgcccagcga gtaagcctat aaaagtaata
     4621 ttaccaatta agacgacgct aatagcagca aggctacttg ccagcatcaa agtatatatt
     4681 ctttcctttt ttaaatgaag ccctagagcg agagcagtct gctcgtttag gttcataata
     4741 ttaaggtggc gactgcgact atatgctaat ccccataaca aaatgagaat cgggataaaa
     4801 gtgataatgg tagtccagga actaattgta atttttccac taagccattc aacgatcata
     4861 tccatttttt gattattaga ctgactaata atagaaatca taacgcctga aatcattgta
     4921 gatattccga taccagtgat gattaatcgt gtagggctaa tattgtgatt tcttctgtat
     4981 gacataagat agacaagaaa aatagttacc aaccctccaa acatagcaaa taggggtaaa
     5041 aagtataata tagttggatt gctaacatta aatgttccta tggttagagc gattactaat
     5101 ccagctccag cattgatacc aagaataccg gaatcagcta gtggattttt ggttaatgtc
     5161 tgtaatagca acccagcaat tcctaaagaa gctcccccaa agatacagac aataatacga
     5221 ggtaaacgaa tgttagtaat gataaaaagg aaggcatcat cagattttcc acttattagt
     5281 tttaaaacgt caataacgga agtattagca tagccaagtg aaattgatac tagtattaat
     5341 aatagtagga taatgacaga tgatattaaa acaaaaggag ctttattttt ttgaatcata
     5401 gatttttccc ttttctaatt aaccatagga aacatggaag ccctacgata ctgattatag
     5461 aactaattgg tgtctctgaa gggggattta taatacgaga tgataagtct acccagatca
     5521 tgaaagtagc ccctgaaaat cctattaaag gtaataacag acgataatct ttgggaataa
     5581 aaagcttaat aaaatgtgga ataatcaatc caatgaaaga aacggttcct atcaacgcga
     5641 ctgatgaagc cgataaaaac aaaacaataa gtaatagaaa cgcagtcatt aattgagttt
     5701 tttgtcccaa tgctttggcc acagattcat taaggctcaa gatagttaat tgatgtgcta
     5761 ggagttgtga aataagtaaa cctaaaataa tgataggtgc tattatgatt agcattttcc
     5821 agttaatttg ggataaacca cctgcttgcc aaccgataac agttcgattg agtttaaagt
     5881 ataatgtaac cacttgtcca acagacgtaa aaagtgtgga gatcatcgct ccagctaaaa
     5941 tgagtcgtag ttggtggtaa cccttttgct ttgtataaga gagtgtgaag actaaaagac
     6001 aagaaatgac agaccctagt aagcagacaa tgagaatcgt actatagtgc atgctcccta
     6061 aaaaagcata tgcaacaaca agggctaacc cagctccggc gttaatacct agtagtccag
     6121 gatcagcaat ggcgtttcga gtgactccct gcataatagc acctgcttgt gacatagctg
     6181 cacctactaa gatagctgca attatgcgtg gtagtctaat gtcaaaaata acatcctgag
     6241 cattagagtt ttttaaggga ttttttaaaa cagttattaa atcactagtc ttgaaattga
     6301 tagcaccaaa tcgtaagctg gcgtaacaac caataagaaa tagtataagt gttatgaaaa
     6361 aaacaagcca aaaatgattt gacttatttt tttgaacgag tttttttgaa gctttaaaca
     6421 taagaggagc tttcttttaa tttgtatttt ctttgatagc ctttgtaaat gattttaatt
     6481 gagcttctaa agatagaggg tcagagaaat aaaacacgtc gtagttactt tctatgatgt
     6541 gccctttttt gacagctggt aaattcttcc agacatcact ttctttaagt gatgaagctg
     6601 cttttttagt cgttttgttt atattaacaa gggcataatc tccaacgtaa tcaccgattg
     6661 cttcttgcga aacggtaaac cacccttttt taaagacatc ttttttgact ttttctgggg
     6721 cagcataacc tagtgaatca tagattagtt ctccaccgcg tccaaaatta ttaccatata
     6781 aatagatatt tttatcataa aaatccataa tagtaaaagt agtgttaggc tttaagatat
     6841 ggtgtaaatc ttttttggca gcgagagttt tagttttcca ttggctaacc cactgattag
     6901 cttctttttc tttaccgaat actttcccca aggctggcat catatctaaa taattttgtg
     6961 caccatattt aataactaaa gttggtgcaa tttttttcag agtattgatg tttggatctt
     7021 gatcgaaaac catgattaaa tcaggttttt gtgcggcaat agcttctgta tcatcagcag
     7081 ttaatttttt agcttctttc agttgcttac caaaaacggg gctatctttt tctaagtcta
     7141 aactgtaact tgaaacatta acacctagtt ttaataaata cccagtgtaa gaatatgtaa
     7201 aattaattac ttttttcgga ttttcaggaa tttttccata ataggtgaag ccttcaattt
     7261 taggcatttt agaaatagtt gttttagtgc tttcttcttt agtttgttgt ctgcaagata
     7321 ccaaaaagaa ggtcagtagt gtgaggacaa taattccaat ttttttcata agataacatc
     7381 tcctaaataa tattaatttt gatcagttga taattgataa gataacaaaa caggttgttt
     7441 actaataggg tcctgaatga tatgagcatc aatcttaaaa atatctttga taatatcttt
     7501 agtcatgatc tgactaggac taccttcata gataatttta ccggttttca ttgcaactag
     7561 ataatcagaa tagcgcgcag acaggtttag gtcgtgtagg accataataa ttgttttttg
     7621 cgtttcgtta ttaagttttt tgagcaattc taagacttct aattgatgat tcatgtcaag
     7681 ataagttgta ggttcgtcta aaaagatagt atctgtatct tgtgctaaag ccatagcaat
     7741 ccatactttt tgcctctgac ctcctgaaag gtcatcaaca tctctattag caaattgcgc
     7801 tacgttagtc atttccatcg cccaatggat tttactacga tcatcatttg tcaaattgcc
     7861 aaggtatttt tggtgtggga accgtccata agatattagt tcataaactg ttatcccttc
     7921 agttgcttct aacacctgtg ggagaagtgc aatcttctta gcaatttcct ttgtgggtaa
     7981 atgtgcaata tcatgaccat caatagtgat agtaccatct tttattttat ggatccgtgt
     8041 gagagccttc aaaagagtag actttccaca tccattggca ccaataatcg ttgttatttt
     8101 ttggttaagt atactgaggg aaagattatt gatgatttct ttctgatcgt aagatacaat
     8161 aatgttttct gattttatgt gagacataga gaacctttct agcaaaaaat taagttcatt
//
LOCUS       DATRUK010000069         8189 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.069, whole genome shotgun sequence.
ACCESSION   DATRUK010000069 DATRUK010000000
VERSION     DATRUK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8189
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.069"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(123..1319)
                     /locus_tag="VBE34_001697"
     CDS             complement(123..1319)
                     /locus_tag="VBE34_001697"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEO7264397.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILEQDPDAHVAAE
                     TAVYTGSVHVFGEISTTAYVDINRVVRNTIAEIGYDKAEYGFSAESVGVHPSLVEQSP
                     DIAQGVNEALEVRGSLEQDPLDLIGAGDQGLMFGFAVDETPELMPLPISLAHQLVKKL
                     TDLRKSGELTYLRPDAKSQVTVEYDDNDQPIRVDAVVISTQHDPNVTNDQLHKDVIEK
                     VINEVIPSHYLDDQTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKVEVQLAYAIGVAQPVSVRVDTFGTGVIA
                     EADLEAAVRQIFDLRPAGIINMLDLKRPIYRQTAAYGHMGRTDIDLPWERVDKVQALK
                     DFIASK"
     gene            1504..2439
                     /locus_tag="VBE34_001698"
     CDS             1504..2439
                     /locus_tag="VBE34_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEO7264398.1"
                     /translation="MKTYEKIYQILSKNNNFISGETMANQLNISRTAIWKGIKTLEEL
                     GLEIESVTNKGYRLVSGDILLPEQLEQEIGIKVSLNNNSASTQLDAKMGIESKLKTPH
                     LFLAPNQKKAKGRFDRPFFTSNQGGIYMSLLLQPNVPIEDIKPYTVMVASSAVKAISR
                     LTGITPEIKWVNDIYLDNKKIAGILTEAIASVESGLVTNVIIGLGINFYIKEFPRALT
                     KRAGSLFTEQPTITRNQLITEIWNLFFNIPLEDHLKVYREKSLVLDKTVSFMDGQTMY
                     SGKAIDITDKGYLVVELDDGQLKTLRSGEISLSSW"
     gene            complement(2420..2614)
                     /locus_tag="VBE34_001699"
     CDS             complement(2420..2614)
                     /locus_tag="VBE34_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEO7264399.1"
                     /translation="MTKRQFIFMALLCSFETYFFNQSVMDGSWIFAIFWGVLLLRDLQ
                     KVYAISKFTKELIKSTKKKD"
     gene            complement(2703..4367)
                     /gene="dnaX"
                     /locus_tag="VBE34_001700"
     CDS             complement(2703..4367)
                     /gene="dnaX"
                     /locus_tag="VBE34_001700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEO7264400.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVSSKKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVNGEPCNHCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKLLAIRDHLAQILDKEAISYDLDALTLVARRAEGGMRDALS
                     ILDQALSLAKDNHISLDVAEEITGSISLSAIDDYVSNILAHDTTEALAKLEVIFDSGK
                     SMSRFATDLLMYLRDLLVVQAGGENSHSSDTFMANLDVKQDILFEMIDKVTSVLPEIK
                     NGSHPKVYAEMMTIQLSEMVEKNSSNIPADVTAELDSLRRELKSLKNEMSQLSRADQS
                     SSTQKVKVNNKTFTFKVDRTKILTIMEETVVDSQRSREYLEALKSAWNEILDNITAQD
                     RALLMGSEPVLANSENAILAFDAAFNAEQAMKRTDLNDIFGNIMSKAAGFSPNILAVP
                     RNDFNQIRSDFAKKMKAQKTETEPEVNHQIPEDFSYLAERIAIVED"
     gene            complement(4367..4864)
                     /locus_tag="VBE34_001701"
     CDS             complement(4367..4864)
                     /locus_tag="VBE34_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEO7264401.1"
                     /translation="MNKSKKIENYQLLLLQAQALFSDETNALANLSNASAMLNAMLPN
                     SVFTGFYLFDGEELILGPFQGGVSCVHITLGKGVCGESAQTAKTLIVDDVTKHANYIS
                     CDSKAMSEIVVPMFKNGKLLGVLDLDSSLVADYDEIDQEYLEKFVGILVEHTIWNLDM
                     FGVEK"
     gene            complement(4951..5580)
                     /gene="udk"
                     /locus_tag="VBE34_001702"
     CDS             complement(4951..5580)
                     /gene="udk"
                     /locus_tag="VBE34_001702"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEO7264402.1"
                     /translation="MRKKPIIIGVTGGSGGGKTSVSRAILSNFPDQKIIMIEHDSYYK
                     DQSHLTFEERVKTNYDHPLAFDTNLMIEQLNELIEGRPVDIPVYDYTKHTRSDRTIRQ
                     EPQDVIIVEGILVLEDQRLRDLMDIKLFVDTDDDIRIIRRIKRDMEERDRSLDSIIEQ
                     YTEVVKPMYHQFIEPTKRYADIVIPEGVSNIVAIDLINTKVASILNEAK"
     gene            5725..6807
                     /locus_tag="VBE34_001703"
     CDS             5725..6807
                     /locus_tag="VBE34_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7264403.1"
                     /translation="MITKFPDQWQDKLTQRQFDDLTDIQNKLFQPITDGDNILGISPT
                     GTGKTLAYLFPTLLKLQPKKSQQLLILAPNSELAGQIFDVTKEWAEPLGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTAGRVFELVKLKKIKMMNINTIVLDEFDELLGDSQYHFV
                     DNIINRVPRDQQMIYISATNKLDNSKLADNTITIDLSDQKLDTIKHYYITVDKRERTD
                     LLRKFSNIPDFRGLVFFNSLSDLGACEERLQFNRASAVSLASDINIKFRKVILEKFKN
                     HDISLLLGTDLVARGIDIDNLEYVINFDIARDKETYTHRSGRTGRMGKEGCVITFVTH
                     KEELKQLKKYATVAELVLHNQKLHLK"
     gene            complement(6839..8092)
                     /locus_tag="VBE34_001704"
     CDS             complement(6839..8092)
                     /locus_tag="VBE34_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO7264404.1"
                     /translation="MKKLIIGIFSLIIIAILAWQGFSFLKHKEIKLQQAVVEKEIRIA
                     EKTVEVVKRQKTERVLFLEPKGYDKSLSADILKWNQKSFEHKKFYDNQYIILRPQLAD
                     SNFANVKKLSIYQILYQKEKGSMFQKSSRLLRTYLLDQNKKPFELDELLAHNISGFKA
                     ILENIAPGTQLKEHDSNKEFLKTGRVTDGLDVKDGKLIINDLKLPLDKLYNVIDESYL
                     KSSDLDLVSNLKAKAPRVALTFDDGPNEKTTPKALEILKRYNAKATFFIMGQSAVGHT
                     DILQRMHAEGHEIGNHTWDHPNLTKLPAEKIKEEIHKTNDLIMKATGQKPVYLRPPYG
                     ATNATVKTVTGLKEMLWSVDTEDWKNHNTQAMMTNIKKQLRPGGVILMHDIHQTSIDA
                     LPTIMDYLTTQGYYFVTVGELYSTP"
BASE COUNT         2681 a         1533 c         1276 g         2699 t
ORIGIN      
        1 acaccatatt ttcaagaaat atgcagtatt tcctatactt aaccagttta ttataagatt
       61 ttataaatgt tctataaatt atcatgagca aaaaaagcgt aaactttcgt ttacgctttt
      121 tattatttgc tagcaataaa atcctttaag gcctggactt tatcaactcg ctcccatggc
      181 aaatcgatat cagtacgtcc catatgccca taggctgctg tttgtcgata aataggtcgt
      241 ttgaggtcca acatattaat aatgcctgct ggtcgcaaat caaatatttg gcgtacagct
      301 gcctctaggt ctgcttctgc tataacacct gttccaaatg tatctacacg cacagagaca
      361 ggctgagcaa caccaattgc ataagcaagt tgtacttcta cttttttagc taaatcagct
      421 gcaacaatat tttttgcaat ataacgagct gcataagatg ctgaacgatc tactttagta
      481 gcatctttac ctgaaaatgc accaccacca tgacgagaat aaccaccata agtatctaca
      541 ataatcttac gacctgttag tcctgaatct ccttgagggc ctccaataac aaagcgccct
      601 gtaggattga taaagaattt tgtttggtca tctaaataat ggcttggaat aacttcattg
      661 ataactttct caatcacgtc cttatggagt tggtcattag ttacattagg atcatgctgt
      721 gtcgaaataa ctactgcatc cacacgaatt ggttgatcat tatcatcata ttcaaccgtt
      781 acttgagatt ttgcatctgg tcgaagatag gtgagttccc cagacttacg tagatctgtc
      841 aatttcttaa ctaactgatg agctaaagaa attggcaatg gcattaactc aggtgtttca
      901 tcaacagcaa acccaaacat caaaccttgg tcacctgcac caattaaatc taaaggatct
      961 tgttcaagag aaccacgtac ctcaagagct tcattgactc cctgtgcaat gtctggcgac
     1021 tgttcaacga gtgacggatg cactccgaca gattcagctg agaagccata ttcagcttta
     1081 tcatagccaa tttcagcgat tgtgttacgg acaactcggt taatatcaac ataggctgtc
     1141 gttgatatct cgccaaatac atgaaccgaa cctgtataaa cagctgtttc ggctgctacg
     1201 tgggcatctg gatcttgctc taaaattgca tctaaaatcg catcggaaat ttggtcagca
     1261 atcttatcgg gatgtccctc agatactgac tcagacgtga aaagtttacg ttctgacata
     1321 aaaatgtccc ccctaaaatg taaaatgtgt cgagttacaa agaactagcc tctaggctac
     1381 cttttcggga ctcatatagc tattgtacca ctatttcatc ttttgtgaaa taagttacca
     1441 ataaataaaa aattatctct attttcttta gtcttttttc tcgatttata ctatactaga
     1501 tttatgaaaa cctacgaaaa aatttatcaa atactatcaa aaaataacaa ctttattagc
     1561 ggagaaacta tggcaaatca acttaatatt tcccgcacag ctatttggaa aggtataaaa
     1621 acgttagaag aattaggcct agagattgag tctgtcacta acaagggcta ccgattggta
     1681 tcaggtgaca tattattacc agaacaactc gaacaagaaa ttggtattaa ggttagtctt
     1741 aataataata gtgcttcaac ccaactagat gctaaaatgg ggatagaaag caaattaaag
     1801 actccacacc tttttttagc tcctaatcaa aaaaaagcta aaggacgttt tgatagacct
     1861 ttctttacat caaatcaagg cggcatctat atgtctcttt tattacaacc taatgtaccg
     1921 attgaagata taaaacctta tactgtcatg gtagcctcta gtgctgtaaa agctatctca
     1981 cggttaacag gaattactcc tgaaattaag tgggtcaatg atatttattt agataataaa
     2041 aaaatagctg gtattttaac cgaggctatc gcttctgttg agagtggatt ggtaacgaat
     2101 gttattatag gattaggtat caatttctat atcaaagagt tccctagggc gttaactaag
     2161 cgcgcaggta gtctctttac agagcaaccg accataacta gaaatcaatt gattacagaa
     2221 atctggaatc tctttttcaa tattccacta gaggatcact taaaggtata tagagaaaaa
     2281 tccctagtct tagataaaac cgttagcttt atggatggtc aaacaatgta ttctgggaaa
     2341 gctattgata ttactgataa aggatattta gtggttgagt tagatgatgg tcagttgaaa
     2401 acacttcgca gtggcgaaat cagtctttct tcttggtaga tttgataagc tcttttgtga
     2461 attttgaaat cgcatacacc ttttgaagat ctcgaagtaa taaaactccc caaaaaatag
     2521 caaaaatcca ggagccatcc ataacggatt gattgaaaaa ataagtttca aaagagcata
     2581 aaagcgccat aaaaataaat tgtcgttttg tcatatcacc attataacag aatctactaa
     2641 ataattaaaa aacttatcaa aaaaatagtt gcctgttacg ggcaactatt tttagttacg
     2701 ttttaatctt caacgattgc tattcgttct gctagataag aaaagtcttc tggaatctga
     2761 tgattaactt caggttctgt ttccgtcttt tgagctttca ttttctttgc aaaatcgctc
     2821 cgaatttgat taaaatcatt tctaggtaca gctaaaatat taggtgaaaa acctgctgct
     2881 ttactcataa tattaccaaa tatatcattg agatcggtac gtttcatagc ttgttcagca
     2941 ttaaaggccg catcaaatgc caaaatagca ttctcgctat tagctaagac aggttcagat
     3001 cccattaata aggctctatc ttgagccgta atattatcta atatctcgtt ccaagcactc
     3061 tttaaagctt ctaaatattc acgtgaacgt tggctatcta caaccgtttc ttccataatg
     3121 gtcaaaatct ttgtacggtc aactttgaat gtgaatgtct tattattaac ctttactttt
     3181 tgagttgatg aactttgatc tgctcgagag agttggctca tttcgttttt taacgatttt
     3241 aactctcgac gaagactatc taattctgct gtcacatctg cgggtatgtt tgacgaattt
     3301 ttttctacca tttcagacaa ttgaatggtc atcatctctg cataaacttt aggatggcta
     3361 ccatttttta tctcaggaag aacagatgtc actttatcaa tcatttcaaa taaaatatct
     3421 tgtttaacat caagattggc cataaaagta tcacttgaat gactattttc accacctgct
     3481 tgaacaacaa gtaaatctct taagtacatc aataaatctg ttgcaaaacg actcatactt
     3541 ttgccactat caaagattac ttcaagttta gcgagtgctt cagttgtatc gtgagccaat
     3601 atattcgata cataatcatc tatagcagat aaggaaatgc tccctgtaat ttcttctgct
     3661 acatctaaag aaatgtggtt atctttcgct aaactcaagg cttgatcgag aattgataaa
     3721 gcatcacgca taccaccttc ggctcgtcta gcgactagtg tcagagcatc caaatcatat
     3781 gaaatagctt ctttatctaa aatctgtgct aaatgatctc gaatagctaa taatttaatc
     3841 gctttaaatt caaagcgctg gacacgagat aagattgttg cagggatttt atggagttct
     3901 gttgttgcta atataaaaac aacattttct gttggctctt caagtgtttt caatagtgca
     3961 ttgaaggcac ctgtagacaa catatgaact tcatcaataa tataaacttt ataggttgcc
     4021 ctacttggag cataagttga tttgtccctg atatcacgaa tttcatcgac accattattt
     4081 gacgcagcat caatttcaat gacatcctct aaactaccat ttgtaatatc tcgacaaata
     4141 tcacaatgat tacaaggttc gccattaact tgatttgggc aattcatcgc ttttgcaaat
     4201 attttagcag cacttgtttt tccagttccc ctaggtcctg aaaaaaggta agcatggcta
     4261 atttttttgc tggacacagc ttgctttaaa gtagttgaaa taacactttg tccaaccatt
     4321 tcatcaaatg tttggctacg atatttccga taaagggcct gatacattac ttttcaactc
     4381 caaacatatc caaattccaa atcgtatgtt ctactagaat acctacaaat ttttctaagt
     4441 attcttgatc aatctcatca taatctgcta ctaaagaaga atctaaatct agaactccta
     4501 gaagtttgcc atttttaaac atgggtacta cgatttcact catagctttt gaatcacagg
     4561 agatatagtt agcatgcttt gtaacatcat caacaatcag cgtcttagca gtttgtgcag
     4621 attcaccaca aacacctttt cctaaagtaa tatgcacaca tgatacacca ccctgaaaag
     4681 ggccaagaat taactcctct ccatcaaata aataaaagcc tgtaaataca gaatttggaa
     4741 gcatagcatt tagcatagct gaagcatttg ataagttggc aagagcattt gtttcatctg
     4801 agaatagagc ttgcgcttgt agtaataata attgataatt ttcgattttc ttagacttgt
     4861 tcatagcact tattatagca taatttgaaa gttactaaaa ctaaataacc atcctatata
     4921 aaacaaaaaa ataaacacaa accattgtgt ttattttgct tcatttaaga tgctagccac
     4981 tttggtattg attaaatcaa tagcgacaat attacttact ccttctggaa ttacaatatc
     5041 cgcataacgc tttgttggtt cgataaattg atgatacata ggttttacaa cttctgtata
     5101 ctgttcaata atactatcta aacttctatc gcgctcttcc atatcacgct taatacgacg
     5161 aataatacga atatcatcat ctgtatcaac aaataatttg atatccatta aatcacgcaa
     5221 acgctgatct tctaatacca aaattccctc aacaataatg acatcctgag gttcttggcg
     5281 gatagttcta tcactacggg tatgttttgt gtaatcataa accggaatat cgaccggacg
     5341 cccctcaatc aattcgttca attgctcaat catcaaattg gtatcaaaag caaggggatg
     5401 atcataattg gtttttacac gttcttcaaa agtcaaatga ctttgatctt tatagtaaga
     5461 atcatgttca atcatgataa ttttttgatc tgggaaattt gataaaatcg cccgagaaac
     5521 actggtttta ccaccaccag atccacctgt aacaccaata ataataggtt ttttacgcat
     5581 ttgtcactcc aaattaaatt tatcctctat ctattgtact ataaacttat tttttttaaa
     5641 gtctaaattt tattgaaaac gctctaaaaa taaaaagcat gttataataa caatgacaac
     5701 atataaaata acaagaggat tcttatgata acaaaatttc cagaccaatg gcaggataaa
     5761 ttaacccagc gtcaatttga tgatctaaca gatattcaaa ataaactttt tcaacctatt
     5821 actgatggtg ataatatcct tggaattagc cctacaggta ctggtaaaac tctagcatac
     5881 cttttcccaa cgcttcttaa gttgcaacct aaaaagagcc aacaactctt aattttagct
     5941 ccaaattcag aattagctgg acaaatcttc gacgttacaa aagaatgggc tgaaccatta
     6001 ggactgacag cacaactatt cctttcaggg tctagtcaaa aacgccaaat tgagcgacta
     6061 aaaaaaggac cagaaatcct tataggaact gctggacgcg tatttgaact tgtgaaacta
     6121 aaaaaaataa aaatgatgaa cattaatact atcgtccttg atgagttcga tgaattactc
     6181 ggcgactcac aatatcattt tgtggacaat attattaatc gcgttcctcg tgatcagcaa
     6241 atgatttaca ttagtgccac caacaaactg gacaatagta aacttgccga taacactata
     6301 acaattgacc tatctgatca aaaacttgat actataaaac attattatat tacagttgat
     6361 aaaagagagc gtactgactt acttcgtaaa ttttcaaata ttcctgattt tagaggtctt
     6421 gtctttttta acagcttatc agacttaggt gcatgtgaag aacgtttaca atttaatagg
     6481 gcctcggcag tttctttagc aagcgatatt aatattaaat tccgtaaagt tatcttagag
     6541 aaatttaaaa accatgatat ttcattactc ctaggaactg atttggtagc tcgtggcatt
     6601 gatatcgaca acctagaata tgtgattaat tttgatatcg cacgcgacaa agaaacatat
     6661 acacatcgtt ctggacgaac tggtcgtatg gggaaagagg gatgtgttat tacatttgta
     6721 acacataaag aagagctcaa acaacttaaa aaatacgcca ccgttgcaga acttgtactt
     6781 cacaatcaaa aattacactt aaaataaaaa agaaacaagt aacttgtttc tttttttatt
     6841 aaggcgttga atataattca cctacagtaa caaaataata accctgggtt gtcagataat
     6901 ccataattgt aggcaaagca tctatggatg tctggtgaat atcatgcatc aaaatcactc
     6961 ccccgggacg taattgtttt ttgatattgg tcatcattgc ttgtgtatta tgatttttcc
     7021 agtcttctgt atcaactgac cacaacattt ctttcaatcc tgtcactgtt tttacagtag
     7081 catttgttgc tccgtaaggg ggacgtaagt acactggttt ttgtcctgtt gctttcatga
     7141 ttagatcatt agtcttatga atctcttcct tgattttctc cgctggtagt ttggttaaat
     7201 taggatgatc ccatgtatga ttaccaatct catgaccttc agcatgcatc ctttgtaaaa
     7261 tatctgtgtg ccctacagca ctttgtccca ttataaagaa agtcgcttta gcattatatc
     7321 ttttaaggat ttctaaggct ttaggagtcg ttttttcatt tggtccatca tcaaaggtaa
     7381 gtgcaacacg tggtgcttta gcttttaagt tagaaactaa atctagatca cttgatttta
     7441 aatatgactc atcgatcaca ttatataatt tatcaagtgg taattttaaa tcattaataa
     7501 ttaacttacc atctttaaca tctagcccat ctgttactct tccagttttt aaaaattctt
     7561 tattgctatc atgttccttt agttgagttc ctggagctat attttctaaa atagccttaa
     7621 atccagatat attatgagct aaaagctcat ctaattcaaa aggtttctta ttttgatcaa
     7681 gtaagtaggt tctcaaaagg cgacttgatt tttgaaacat acttcctttt tctttttgat
     7741 ataagatttg ataaatggat aattttttaa catttgcaaa gttagaatct gctaattgag
     7801 gacgtagaat aatgtattga ttgtcataaa attttttatg ctcaaaactt ttttgattcc
     7861 actttaaaat atcagcgctt agacttttgt catatccttt aggttcaaga aaaagaacac
     7921 gttcagtttt ctgtcttttc accacttcta ccgttttctc ggctatgcga atttcttttt
     7981 caacaacagc ttgttgaagt tttatttcct tatgtttcaa aaacgaaaaa ccttgccaag
     8041 caagaatcgc tataataatt agactaaaaa tacctataat taatttcttc atataatgac
     8101 tcttttgtta ttttttatga caattatatc acaaaaataa aaccacttta gaagattata
     8161 taattcttta tttttaatgt aaaagaggt
//
LOCUS       DATRUK010000070         8088 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.070, whole genome shotgun sequence.
ACCESSION   DATRUK010000070 DATRUK010000000
VERSION     DATRUK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.070"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            17..1153
                     /gene="dnaN"
                     /locus_tag="VBE34_001705"
     CDS             17..1153
                     /gene="dnaN"
                     /locus_tag="VBE34_001705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEO7264405.1"
                     /translation="MIHFSINKNFFLHALTVTKRAISHKNAIPILSTVKIEVTRDAII
                     LTGSNGQISIENTIPASNENAGLLVTNPGSILLEAGFFINIISSLPDVTLEFTEIEQH
                     QIVLTSGKSEITLKGKDVDQYPRLQEMTTDTPLTLETKLLKSIINETAFAASQQESRP
                     ILTGVHLVISQNKYFKAVATDSHRMSQRTFQLEKSANNFDLVVPSKSLREFSAVFTDD
                     IETVEVFFSDSQMLFRSENISFYTRLLEGNYPDTDRLLTNQFETEIIFNTNALRHAME
                     RAYLISNATQNGTVRLEIQNETVSAHVNSPEVGKVNEELDTVSLKGDSLNISFNPTYL
                     IESLKAVKSETVTIRFISPVRPFTLTPGEDTEDFIQLITPVRTN"
     gene            1211..2104
                     /locus_tag="VBE34_001706"
     CDS             1211..2104
                     /locus_tag="VBE34_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEO7264406.1"
                     /translation="MMSNMTLYIIANPHAGNKNASTIVGKIQELYHTEDISVFYTEQK
                     DDEKKQVINILRSFKESDHLMIIGGDGTLSKVMTYLPQHIPCAYYPVGSGNDFARALK
                     IPNLKETLTAIQTERLKEINCFIYDKGLILNSLDLGFAAYVVWKASNSKIKNILNRYR
                     LGKITYIVIAIKSLLHSSKVQVLVEGETGQRIKLNDLYFFALANNTYFGGGITIWPKA
                     SALTAELDMVYAKGHTFLKRLSILLSLVFKRHTTSKSIKHQTFKAMTVYFPKNSLIEI
                     DGEIVELDQISLKCQKRYLYM"
     gene            2114..2311
                     /locus_tag="VBE34_001707"
     CDS             2114..2311
                     /locus_tag="VBE34_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEO7264407.1"
                     /translation="MYQVGSLIEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            2514..2630
                     /locus_tag="VBE34_001708"
     CDS             2514..2630
                     /locus_tag="VBE34_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264408.1"
                     /translation="MDNLIRLAEIFDVSLDEHVLAKTNEVKVERIYEKNLDL"
     gene            2874..3989
                     /gene="ychF"
                     /locus_tag="VBE34_001709"
     CDS             2874..3989
                     /gene="ychF"
                     /locus_tag="VBE34_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEO7264409.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEEEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ADPDDIDYVNQIRAFAETESAEVVVISARAEEEISELDDEDKLEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LIQYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            4073..4648
                     /locus_tag="VBE34_001710"
     CDS             4073..4648
                     /locus_tag="VBE34_001710"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEO7264410.1"
                     /translation="MVKMIVGLGNPGSKYNDTKHNIGFMAVDRIVKKLDVNFTEDKNF
                     KAEIGSDFINGEKIYFIKPTTFMNNSGIAVKALLTYYNISIKDMIIIYDDLDMEVGKI
                     RFRQKGSAGGHNGIKSIIAHLGTQEFDRIKVGIGRPNGRMTVINHVLGKFDKNDEIMI
                     SNTLDKVDNAVNYYLQTNDFQKTMQKYNGLK"
     gene            4645..>8088
                     /gene="mfd"
                     /locus_tag="VBE34_001711"
     CDS             4645..>8088
                     /gene="mfd"
                     /locus_tag="VBE34_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEO7264411.1"
                     /translation="MNIIELFSQNKVVRTWHSGLVTNSRQLVMGFSGASKAIAIASAY
                     EKLSKKIMVVTATQNDSDKLSSDISSLIGEDNVYQFFADDVPAAEFIFSSLDKSISRL
                     SALRFLKDPEKNGVLITSISGLRLLLPNPEVFSKSQYKFEIGQECYLDKLSKNLVNLG
                     YQKVSQVFSPGEFSQRGDILDIFEMTQEYPYRLEFFGDEIDGIRQFDIDTQKSLKQLE
                     SVQISPADDIILQDADFERAKKKLEGYLVTASEEQRAYLSEVLSTTENHFKHSNIRRF
                     LSIFYEKEWGILDYIPEGTPLFVDDFQKIVDRNAKLDLEIASLLTEDLQQGKSHSSLN
                     YFSDPYKQLRQYQPATFFSNFHKGLGNLKFDKLHHFTQYGMQEFFNQFPLLVDEINRY
                     KKSGATVLLQVDSQKGLNLLQENLKEYGLDLIISDKNDIVPKESQLIVGHLSNGFYFA
                     DEKIVLITEREIYHRRVKRKIRRSNISNAERLKDYNELSVGDYVVHNVHGVGKFLGIE
                     TIEIQGIHRDYLTIQYQNADRISIPVEQIELLTKYVSADGKEPKINTLNDGRFKKAKQ
                     RVAKQVEDIADDLLKLYAERSQLQGFAFSPDDNMQNDFDNDFAYVETEDQLRSIKEIK
                     QDMEGNRPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHFENFKE
                     RFSNYPVTVDVLSRFRSKKEQTDTLKRLSKGQVDIIIGTHRLLSQDVVFSDLGLIVID
                     EEQRFGVKHKEKLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLETNPGLVREAIIREIDRGGQVFYVYNKVDTIEQKVSELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFVENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEISEKRLDAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIATKQGKSLIRQKGNVELALQIDAYLPAEYISDERQK
                     IEIYKRIRELETRADYEALQDELIDRFGEYPDQVAYLLEIGLLKAYLDLAFTELVERK
                     GNEISIIFEKASLKYFLTQDYFEALSKTQLKARISETNGKMEVVFNIKHKKNYEIIEE
                     LL"
BASE COUNT         2813 a         1153 c         1521 g         2601 t
ORIGIN      
        1 atataaagga tatactatga ttcatttttc aataaataaa aatttcttct tgcatgctct
       61 aacagtaacc aaacgagcta ttagtcataa aaatgcgatt ccaatccttt caactgttaa
      121 aatagaagtg actagagatg ctatcatttt aacggggtca aatggacaaa tttcaattga
      181 aaatactatt cctgcttcaa atgaaaatgc aggtttacta gtaacgaatc caggctctat
      241 tttgttagaa gctggtttct ttattaatat tatttcaagt ttaccagatg taactttaga
      301 atttacagag attgaacaac atcaaattgt tcttactagt ggaaaatcag agattacttt
      361 gaaaggtaag gatgtcgatc aataccctcg tctgcaggaa atgacaacag atactccatt
      421 aacattagaa actaaactgt taaaatcaat tattaatgaa actgcttttg ctgctagcca
      481 acaagaaagc cgtccaatct taacaggtgt tcatttggtt atcagtcaaa ataaatactt
      541 taaggctgtt gcgacagatt cacaccgtat gagtcaacgc actttccaat tagagaaatc
      601 ggctaataat tttgatttgg ttgttccaag taaatccctt cgagaatttt cggctgtttt
      661 tacagatgat attgaaactg tagaggtttt cttctcagat agtcaaatgt tatttagaag
      721 tgaaaatatc agcttctata cacgtttgct tgaaggaaac taccctgata ctgatcgcct
      781 cctaactaat cagtttgaaa ccgaaattat ctttaataca aatgctttac gccatgctat
      841 ggaacgtgct tatttaattt cgaatgcaac tcagaacggt actgttcgtt tagaaattca
      901 aaatgaaaca gtctcagctc atgtaaactc tccagaagtt ggtaaagtta atgaggaatt
      961 ggatactgtt agccttaaag gtgatagttt aaatattagt tttaatccaa cttacctaat
     1021 tgaatcttta aaagcagtaa aaagcgaaac agttacgatt cgatttattt ctccagtacg
     1081 tccatttact ttgacccctg gtgaagatac tgaagatttc atccaattaa taactcctgt
     1141 tcgtactaac taaataagaa aactacccaa gttattcttg ggtagttttc ttattctaag
     1201 atatgctata atgatgagta atatgactct atatataata gctaaccccc atgctggtaa
     1261 taaaaatgcc tccactattg ttggtaaaat tcaggagctt tatcatactg aagatatttc
     1321 tgtgttctat acagaacaga aagatgatga aaaaaaacaa gtcattaata tactaaggtc
     1381 ttttaaagaa agtgatcatc taatgattat aggaggagat ggtaccttat caaaagtaat
     1441 gacttatctc ccccaacata ttccgtgcgc ttattatcct gttggttcag gaaatgattt
     1501 tgccagggct ttgaaaattc ctaacctcaa agaaaccctt accgctattc agacagaaag
     1561 attaaaggaa attaattgct ttatttatga taaagggctg attttgaata gtttagattt
     1621 aggttttgca gcctatgtcg tttggaaagc atctaactct aaaataaaaa atattttgaa
     1681 tagatatcgt ctaggaaaaa taacttacat tgttatagct atcaaatcat tattacattc
     1741 ttctaaagta caagtattag tggaaggaga gacaggacag cggataaaac taaatgacct
     1801 ttattttttc gcattagcaa ataacactta ttttggaggt ggcattacta tttggcctaa
     1861 agcaagtgct ttaactgcag agttagatat ggtttacgct aaagggcata cttttctaaa
     1921 acgattaagt attttattat ctttggtttt taaaaggcat actacctcta aaagtataaa
     1981 acaccagact ttcaaagcta tgaccgtata ttttcctaaa aatagtttga ttgaaataga
     2041 tggcgaaata gttgagttag atcagattag tttaaaatgt caaaaaaggt atttatatat
     2101 gtaaggagaa aagatgtatc aagttggatc acttattgaa atgaaaaaac ctcatgcttg
     2161 tgtaattaaa gaaactggta aaaaggctaa tcaatggaag gtactgagag taggagctga
     2221 tattaaaatt cagtgtacta actgtcagca tgtgattatg atgagtcgtt atgattttga
     2281 gcgaaaatta aaaaaagtac tgcaaccatg aaagtccttt taaacaaata attatagcta
     2341 tatttgcaac caatactttc ctaaataatt gctagtatgc cgttggaaaa ttagctattc
     2401 taatgttatc gaaagaagaa aggtggccat tgacaatgac gaaagttgca gaacagttgt
     2461 ttatttttcg gtaagtcata tcaaaatggg aaaatgggga taccacacca gatttggata
     2521 atttaatcag gttagctgag atttttgacg tgagcttaga tgaacatgtt ttagctaaaa
     2581 caaatgaagt taaagttgaa cgtatttatg aaaaaaatct tgatctgtaa aaatataata
     2641 aaatttattg gtttattttt cgaaatatta ttcgatctct actaattatc ttagctatat
     2701 taactttctt ataagctcta ggtcttccct ttgtttcgga gtggataatt taaatgaagg
     2761 tgaaagagtg gcataatgat aagcaaatct gaaattttct tcttatcata ccgctttttc
     2821 ttctattttt tgatataatg gtcttgattg aaatttgaat ggagactcac taaatggctt
     2881 taacagcagg tattgtaggc ttaccaaatg ttggtaaatc aactttattt aacgcaatta
     2941 caaaagcagg agcagaagct gctaactatc cttttgcgac tattgatccc aatgttggta
     3001 tggtagaggt accagatgag cgtctgcaaa aattgacaga attgattacg cctaaaaaaa
     3061 cagttccgac aacctttgaa tttactgata ttgcaggtat tgttaaaggt gcttcgaagg
     3121 gggaaggtct aggaaataaa tttttagcta atattcgtga agtggatgct attgttcatg
     3181 tggtacgtgc ttttgatgat gaaaatgtta tgcgtgaaca aggtcgtgag gatgccttcg
     3241 ttgatcctat agcagatatt gatactatta atcttgaatt aatcttagct gatttagagt
     3301 caatcaataa acgttatgcg cgtgttgaaa aaatggcacg aactcaaaaa gataaagaat
     3361 cagtagcaga gttcaatgtt ttacaaaaga ttaaacctgt tttggaagat gggaaatcag
     3421 ctcgtaccat tgagtttaca gaagaggaag caaaagttgt taaaggtctc tttttattaa
     3481 caactaaacc tgttttatat gttgctaatg ttgatgagga caaagtagct gatcctgatg
     3541 atatcgacta tgttaatcaa attcgtgcat ttgcagaaac agagagtgca gaagtagttg
     3601 tgatttctgc tcgagctgaa gaagaaatat ctgaacttga tgatgaagat aagttagaat
     3661 ttttagaagc tattggatta acagaatctg gtgttgataa attaacacga gctgcttatc
     3721 atcttttagg ccttggaact tactttactg ctggtgaaaa agaggttcgc gcttggacat
     3781 ttaagcgtgg tataaaagct ccacaagctg cgggtattat ccattcagat tttgaaagag
     3841 gttttattcg tgcagtaacg atgtcatatg atgaccttat tcaatatgga tctgaaaaag
     3901 cagtaaaaga agctggccgg ttacgtgaag aaggaaaaga gtatattgtt caagatggcg
     3961 atatcatgga attccgtttc aatgtctagt aataaataat caatttgtta acttttaggt
     4021 taaggatatt gttattatcc ttaaccattt ttgcgtttta atggagaaga taatggtaaa
     4081 aatgattgtt ggattaggaa atccaggaag taaatataat gatacaaaac ataatattgg
     4141 atttatggct gttgacagaa ttgtaaaaaa acttgacgtc aactttacag aagataaaaa
     4201 ttttaaagca gagataggta gtgattttat caacggtgaa aaaatttatt ttatcaaacc
     4261 aactactttt atgaataaca gtggtattgc ggtaaaagcc ctgcttactt actacaacat
     4321 ttctattaag gatatgatta tcatatatga tgatttagac atggaagtcg gtaaaatacg
     4381 ttttcgtcaa aaaggatctg ctgggggaca taatggtatc aagtctatta ttgctcattt
     4441 aggtactcaa gaatttgatc gtattaaagt aggtatagga cgtccgaatg gacgtatgac
     4501 agttattaat catgttctag ggaagtttga taaaaatgat gaaattatga tatcaaatac
     4561 tcttgataag gttgacaatg ctgtcaatta ttatttacag acgaatgatt ttcaaaaaac
     4621 aatgcagaaa tacaacggtt taaaatgaat attatagaat tatttagcca aaataaagtg
     4681 gtgcggacat ggcactctgg attagtaact aatagtcgcc aattagttat ggggttttca
     4741 ggagcaagta aagctattgc tatagcatca gcctacgaga agttatctaa aaaaattatg
     4801 gtagtcactg ccactcaaaa cgattctgat aaattatcaa gtgatatatc aagtttaatt
     4861 ggagaagata acgtttatca attttttgct gatgacgttc ctgcagcaga gtttattttc
     4921 tcttcattag acaaatcaat ttcacgactc agtgccttga ggtttctaaa agatccagaa
     4981 aaaaacggtg ttttgattac tagtatcagt ggtttaaggt tactattacc aaatccggaa
     5041 gttttctcga aaagtcaata taagtttgaa atcggtcaag aatgttattt agataaattg
     5101 agtaagaact tagtcaattt aggttatcag aaagtttctc aagtatttag tcctggtgaa
     5161 tttagccaaa gaggagatat tttagatatt tttgagatga cacaagaata tccttatcgc
     5221 ttggaattct tcggagatga aatagatggc atcaggcagt ttgatattga cactcaaaag
     5281 tctcttaaac aattagagtc agtacagatt tcgccagcag atgatattat tttacaagat
     5341 gcagatttcg agcgtgcaaa gaaaaaactt gagggctatc tagtaacagc tagtgaggaa
     5401 caacgcgcct atttgagcga ggttttatct acaaccgaga atcattttaa gcactcaaat
     5461 attcgtcgtt ttctaagtat cttttatgaa aaagaatggg gaatattaga ctatattcct
     5521 gagggaactc cactttttgt tgatgatttt caaaaaattg tagatcgaaa tgctaagtta
     5581 gatttagaaa tagctagcct tctgacggaa gatttacaac aaggcaagtc tcattctagt
     5641 ttgaactatt tttcagatcc ttataagcaa ttaagacaat accaaccagc aaccttcttt
     5701 tcaaatttcc acaaaggatt aggaaatctg aaatttgata aacttcatca ttttactcaa
     5761 tatggaatgc aagaattttt taatcaattt ccattgttgg tcgatgaaat caatcgttat
     5821 aagaaatctg gagcgacagt actgttgcaa gtagattctc aaaaaggttt aaatctcctt
     5881 caagaaaact taaaagaata tgggttagat ttaatcattt cagataaaaa cgatatcgtt
     5941 ccaaaggagt cacaacttat tgttgggcat ctttcaaatg gtttttattt tgcagatgag
     6001 aaaattgttc tgattactga acgtgagatt tatcataggc gtgttaaaag gaaaattaga
     6061 cgcagtaata ttagtaatgc agaacgtcta aaagattata atgaactttc agttggcgat
     6121 tatgttgttc ataatgttca tggcgtagga aaatttttag gtattgaaac aattgagata
     6181 caaggtattc atcgtgatta tctgactatt caatatcaaa atgctgatcg gatctctatc
     6241 cccgtagaac aaattgaact tctaacaaaa tatgtctctg cagatggcaa agagcctaaa
     6301 attaatacgt taaatgatgg tcgcttcaaa aaagcaaaac agagagttgc aaagcaagta
     6361 gaagatattg ctgatgattt gttgaaactt tatgcagagc gtagtcaatt acaaggattt
     6421 gcattttctc ctgacgataa tatgcaaaat gactttgata atgattttgc atatgtagag
     6481 acagaggatc aattgcggtc gataaaagaa attaaacagg atatggaagg taatcgtccg
     6541 atggatcgat tattggttgg agacgttggt tttggtaaga cagaggttgc tatgcgtgcg
     6601 gctttcaaag ctgtcaatga ccataaacaa gttgctgttt tagtcccaac aacagttcta
     6661 gctcagcagc attttgaaaa ttttaaagaa cgttttagta attatcctgt tacagttgat
     6721 gtacttagtc gttttcgtag caaaaaagaa caaacagata cactaaaaag attatctaag
     6781 ggccaagttg atattattat tggaacacat cggcttctct ctcaagatgt tgtattttcg
     6841 gatttagggt taatagttat tgatgaggaa cagcgatttg gtgtaaagca taaagaaaag
     6901 ttaaaagaat taaaaacaaa agttgatgtc ttaactttga cagcgacccc aatacctaga
     6961 acattacata tgtcaatgct tggcattaga gacttatcag tcattgaaac acctccaact
     7021 aatcgttatc ctgtgcagac ttacgttttg gagacaaatc ctggtttagt tagagaagct
     7081 attattcgtg agatagacag aggaggacag gttttttatg tttacaataa agttgacact
     7141 attgaacaga aagtttcaga acttcaggag ttagtaccag aagctagtat tggttttgtt
     7201 catggtcaaa tgtctgaaat tcaattagaa aatacattaa tagactttat caatggagat
     7261 tacgatgttt tagttgcaac gacaataatt gagacaggag tcgatatttc aaatgttaat
     7321 accctctttg ttgaaaatgc agatcatatg ggcttgtcaa ccctttatca attaagaggc
     7381 cgtgtgggta gaagtaatcg cattgcttat gcttacctta tgtacagacc tgataaagtt
     7441 ttaacagaaa tttctgagaa aagactagat gcaatcaaag ggtttacaga attgggatct
     7501 ggttttaaaa tagcgatgcg tgatttatct atacgtggtg caggaaatat tttaggagct
     7561 tcacaaagtg gttttattga ttcggttgga ttcgagatgt attcacagct tctagagcaa
     7621 gcgattgcca ccaaacaagg aaaatcatta attcgtcaaa aaggtaatgt tgaattagcc
     7681 ttacaaattg acgcctattt accagcagaa tatatatcag atgagcgcca aaaaatagaa
     7741 atatataagc gtattcgtga attagaaact agggctgatt atgaagcctt acaagatgag
     7801 ttaatagatc gttttggaga atatcctgat caagttgcat accttttaga aataggactt
     7861 ttaaaagctt atctggatct tgcctttact gaattagtag aaagaaaagg taatgaaata
     7921 agcatcatat ttgaaaaagc atctttgaaa tattttttaa ctcaagatta ttttgaagca
     7981 ctatcaaaaa ctcaacttaa ggctagaatt agcgaaacaa atggtaagat ggaagttgtt
     8041 tttaatatca aacataagaa aaattatgaa attattgaag aacttcta
//
LOCUS       DATRUK010000071         7758 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.071, whole genome shotgun sequence.
ACCESSION   DATRUK010000071 DATRUK010000000
VERSION     DATRUK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.071"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(225..1517)
                     /locus_tag="VBE34_001712"
     CDS             complement(225..1517)
                     /locus_tag="VBE34_001712"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEO7264412.1"
                     /translation="MYIIKNGLIIDPQSGFNQVSDMLIDQGKIKQISKEIDIKGIPII
                     DASNKIVAPGLVDIHVHFREPGQTHKENIHTGALSAAAGGFTTVLMMANTNPTISSSE
                     IVKQVKESAAKEAIKIETVATITKSLNGKDLVNFEELLEAGVAGFSDDGIPLTDTKVL
                     QEAMNLARKHDVVLSLHEEDPSLNGVLGINEHIAQKIYHVCGASGLAEYSMIARDAMI
                     AYQTQAKVHIQHLSSSESVEVVDFAQKLGANLTAEVTPQHFSKTEDLLLTKGANAKLN
                     PPLRLEKDRQALIDGLKSGVISIIASDHAPHHIMEKAADNISQAPSGMTGLETSLALG
                     ITYLVSTKELSMIDFLAKMTCNPAQLYGFDAGYLREGGPADIVIFDQAEERIIKAEFA
                     SKSSNSPFIGDKLKGVVHYTICNGEIVYQKDSHAATLV"
     gene            complement(1529..2158)
                     /locus_tag="VBE34_001713"
     CDS             complement(1529..2158)
                     /locus_tag="VBE34_001713"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEO7264413.1"
                     /translation="MDLARQIAMELLDIQAVYLRPQQPFTWASGVKSPIYTDNRVTLS
                     YPETRTLIENGFVKQIQKHFPNVDIIAGTATAGIPHGAIIADKMNLPFAYIRSKAKDH
                     GVGNQIEGRVYSGQKMVIIEDLISTGGSVLEAVTAAQSQGIEVLGVVAIFTYQLAKAE
                     QAFREADIPLVTLTDYNQLIKVAKVNGYITADQLVLLKKFKEDQMNWQS"
     gene            complement(2171..2872)
                     /gene="pyrF"
                     /locus_tag="VBE34_001714"
     CDS             complement(2171..2872)
                     /gene="pyrF"
                     /locus_tag="VBE34_001714"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEO7264414.1"
                     /translation="MLEKCPIIALDFSDLASVTTFLEHFPKEELLFVKIGMELYYSEG
                     PSIIRYIKSLGHRIFLDLKLHDIPNTVRLSMSVLAKLGIDMTNVHAAGGVEMMKAARE
                     GLGEGPILLAVTQLTSTSQEQMQVDQHINLSVVDSVCHYAQKAQEAGLDGVVASAQEV
                     KQIKKQTNEHFICLTPGIRPPQTNQLDDQKRTMTPEQARIVGADYIVVGRPITKAENP
                     YQAYLDIKEEWNRIK"
     gene            complement(3085..4317)
                     /locus_tag="VBE34_001715"
     CDS             complement(3085..4317)
                     /locus_tag="VBE34_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEO7264415.1"
                     /translation="MQNDMSVVLRAGKLLIESGAEVYRVEDTMKHFAKALQIENFEAY
                     VVSSSIIASGINRYGKQEAKVCNTDGVTANLGRLEAVNNLSRQIAKQDLVSPEEIVKQ
                     LDLIEHQKDYSLLVTLISYFCGAGSFSLALGSSLLDSFSAAVTGLILGYFLNLMESRI
                     HTGFLLTILGSSVVALSANLLYFSGLGEHRSIIILGALMVMVPGAAFVNSVREFSQNN
                     FSTGLALIMSALLICISISAGVAITIEIIPFAEQMTGSFSGVPNTILEIIIRTLMAGL
                     GTIAFSILYHVPKRYFLDLGILGAISWMLYLILWQQFHMDAIAVFFPGLFITYFSRLL
                     AAKRKCPATVFLATSMFPLIPGLSFYRAVYFLLTGADALAMEYFRSCFVTAFTIAIAI
                     SIVQQIPLSFFIRRKMIK"
     gene            complement(4357..5898)
                     /locus_tag="VBE34_001716"
     CDS             complement(4357..5898)
                     /locus_tag="VBE34_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO7264416.1"
                     /translation="MSILEVKNLSHGFGDRAIFENVSFRLLKGEHIGLIGANGEGKST
                     FMSIVTGHLQPDEGKIEWSKYVTAGYLDQHTVLEEGQTVRDVLRTAFDELFKTEARIN
                     DIYISMADDGADVDSLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDSDVT
                     ELSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQDLVRYSGDYTNFESVYAMKKAQLEAAYERQQKEIADLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPSFEFKESRTPGRFIFQAKDLQIGY
                     DRALTKPLNLTFERNQKIAIVGANGIGKTTLLKSLLGIIPPISGNVERGDFIDLGYFE
                     QEVPGGNRQTPLEAVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQSKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKAYKGSILMVCHEPDFYEGWMDDVWD
                     FNQLS"
     gene            complement(6029..6367)
                     /locus_tag="VBE34_001717"
     CDS             complement(6029..6367)
                     /locus_tag="VBE34_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264417.1"
                     /translation="MQIIKRDGQIVEFDPEKIYQAILKAAQTVYVLDESLRQDLAQVT
                     KKVILDLEDAQVERPTINMIQSLVENRLLDAGYIHIAEHYISYRLQRDLERNGYDDQI
                     TVHLHFEKIK"
     gene            complement(6537..7613)
                     /locus_tag="VBE34_001718"
     CDS             complement(6537..7613)
                     /locus_tag="VBE34_001718"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEO7264418.1"
                     /translation="MGYTVAIVGATGAVGTQMIRQLEQSNLPIEQVKLLSSSRSAGKI
                     LHFKDEAIRVEETTKESFYDVDIALFSAGGSISAKFAPYAVKSGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHAMIGHNGIIACPNCSTIQMMIALEPIRQKWGIERVIVSTYQAVSGS
                     GARAVEETKEQLRQVLNDNLSPDQLIATVLPCSSDQKHYPIAFNALPQIDIFTDNDYT
                     YEEMKMTLETKKIMEDATIKVSATCVRIPVLSGHSESIYIETKELASISEIKKAIANF
                     PGAVLQDLPSQQIYPQAINAVGHRETFVGRIRKDLDQENGVHMWVVSDNLLKGAAWNS
                     VQIAETLHKNGLVKPAKELKFELI"
BASE COUNT         2453 a         1542 c         1254 g         2509 t
ORIGIN      
        1 tgataattct tctaaactga caaaatgttc taatgaaagt gtttgtgttt gatgattcat
       61 gataatttct ttctataaat tttagctaga aaaaagcctc ctaaaacaag gaggctctgc
      121 aaaatagtaa gctagatagg gaactaactt actatttgtt aacgcaattc cttgtttgcc
      181 tctctggaca ataccttaaa ggatattaat aagaaatgtt ttacctatac caatgtcgct
      241 gcgtggctat ctttttgata gactatttca ccattgcaaa ttgtgtagtg aacaactccc
      301 ttcaatttat caccgataaa aggagagttg ctggatttgg aggcaaattc tgcctttatt
      361 attctctctt cagcttgatc aaaaataacg atatctgcag gtccaccttc tctgagataa
      421 cctgcatcaa atccatataa ctgtgcagga ttacatgtca tttttgccaa aaagtcaatc
      481 attgagagtt ctttagtaga tactaaataa gtaatcccta aggctaatga tgtctctaag
      541 ccagtcatac cagagggcgc ttgactaata ttatctgctg ctttctccat aatatgatgg
      601 ggagcgtgat cacttgcaat aattgaaata acaccacttt taagaccatc aatgagggct
      661 tgtctatcct tttcaagtcg cagtggtgga tttaattttg cattagcacc cttagttaaa
      721 agtaagtcct ctgtctttga aaaatgttgg ggggttactt ctgctgttaa gttagcacca
      781 agcttctgcg caaaatcaac tacttctact gattctgaac ttgacaaatg ctggatatgg
      841 actttcgcct gtgtttggta agcaatcata gcatctcgcg caatcattga atattcagca
      901 agaccactag ctccacagac atgataaatt ttctgagcaa tatgttcgtt gataccaaga
      961 acaccattta acgatggatc ctcttcatgt aacgatagga caacatcatg ttttctagct
     1021 aagttcatag cttcttgcaa aaccttagta tctgtcaaag ggataccatc gtctgagaat
     1081 ccagcaactc cagcttctaa taattcttcg aaattaacta aatctttacc attcagggat
     1141 ttagtaattg tagcaactgt ttcaatctta attgcttctt ttgctgcact ctccttaact
     1201 tgttttacaa tttctgagct agaaatcgtt gggttagtat tggccatcat aagaactgtc
     1261 gtaaaaccac ctgctgcagc tgataaggct cctgtatgga tattttcttt atgtgtctgt
     1321 ccaggctcac gaaaatgtac atgaatatcg actaatcctg gagctacaat tttattgctt
     1381 gcatcaatga ttggaatgcc tttaatatca atttctttcg atatttgttt aatttttcct
     1441 tgatcaatca gcatgtctga tacttgatta aaaccacttt gagggtcgat aatcaatccg
     1501 tttttaatga tatacatatc tgtcctctct aactttgcca attcatttga tcttctttaa
     1561 attttttcag taataccagt tggtcagctg taatatatcc attaacttta gcaactttaa
     1621 ttaattgatt gtaatccgtc aaagtcacca aaggaatatc agcttcacga aatgcttgtt
     1681 cagccttagc taattgatat gtaaatatcg ctaccacccc taaaacctca ataccttgac
     1741 tctgcgcagc cgtaacagcc tctaaaacag atcctcctgt tgaaatcaaa tcttcaatga
     1801 taaccatttt ttggcctgag taaacacgtc cctcgatttg attccccaca ccatgatcct
     1861 ttgccttcga gcgtatatag gcaaagggca agttcatctt atcagctata atagcgccat
     1921 gtggtatgcc tgcagtcgct gttcctgcga tgatatcaac gtttgggaaa tgtttctgta
     1981 tttgtttaac aaaaccattt tcaatcagtg tccttgtctc aggatacgat aaggttacac
     2041 gattatcagt gtaaattggc gatttgaccc ccgaagccca agtaaagggt tgttggggtc
     2101 ttaagtaaac agcttgaata tctaatagtt ccatggcaat ctgtcttgct aaatccatct
     2161 cagtactcct ttatttaata cgattccatt cttcttttat gtctagatat gcttgatatg
     2221 gattttctgc tttcgttata ggtcttccca caacgatata atcagcccca actatacgtg
     2281 cttgctcagg agtcatcgtt cttttttgat catctaactg attggtctgt ggaggtctga
     2341 taccaggcgt taagcaaatg aaatgttcgt tagtctgttt cttgatttgc tttacctctt
     2401 gagccgatgc gactactcca tctaagcctg cttcttgagc tttttgagca taatggcaaa
     2461 ccgaatcaac tacacttaag ttaatatgtt gatccacctg catttgttct tgtgaagtcg
     2521 aggttaattg tgtcactgct aatagtattg gaccctcccc caaaccttct ctagctgctt
     2581 tcatcatttc gactcctcct gcagcatgga cattagtcat atcaatgcct aatttggcta
     2641 agacagacat agataatcga actgtattag ggatatcatg taattttaaa tccaaaaaaa
     2701 tgcgatgtcc taaggattta atatatctaa taattgaggg accttcggaa taataaagct
     2761 ccattcctat tttgacaaaa agcaattctt cttttggaaa atgttctaaa aaagttgtta
     2821 cagaagccaa atcagaaaaa tctaaggcaa tgatagggca tttttctaac atataattcc
     2881 tctctataga aataataatt gttggaaatg taccagtatg acacaaaaaa ccttgcctga
     2941 gggcaaggtt tcataaaaag ttagcgaaaa agcactcgct atttgactta tgatggtgac
     3001 cttgttcgcc tctctggact cacttaaaga tacatttgta ggtattctac aagtttacaa
     3061 tcattttgtc aaataaatac taaattattt tatcatttta cgtctaataa agaacgatag
     3121 aggaatctgc tgtacaatac tgattgctat agcaattgta aaagctgtta caaaacagga
     3181 tctaaaatat tccattgcta aggcatctgc tcccgtcaat aaaaagtaaa cggctctata
     3241 gaagctaagt cctgggataa gaggaaacat acttgtcgct aaaaagacag tggcaggaca
     3301 cttacgttta gcagctaata gccgtgaaaa ataagtgatg aaaagccctg gaaaaaatac
     3361 ggctatagca tccatgtgaa attgttgcca taagatcagg tagagcatcc aagatattgc
     3421 tcctagtatc cctaagtcaa ggaaataacg ttttggaaca tggtagagaa tagagaaggc
     3481 tatcgtccct aaaccggcca ttagtgttct gatgataatc tcaagaatgg tattaggaac
     3541 accagaaaaa gaacctgtca tttgttctgc aaacggaata atttcaattg taatagccac
     3601 gcctgctgat atagaaatac aaatgagtaa agcagacata atcaaagcta aacctgttga
     3661 aaaattattc tgtgaaaatt ctctcaccga attaacaaag gcagcccctg gcaccataac
     3721 cattaatgcc cctaatatga taatgctacg atgctcacct aatcctgaaa aatataataa
     3781 attagcactt aaagccacta cagaactacc tagaatggtc aaaagaaatc ctgtatgtat
     3841 acgactttcc atcaaattca aaaaatatcc taaaattaag cctgttacag cagcggaaaa
     3901 tgaatctaat aaggaactac ccaaggctaa agaaaaacta cccgctccac aaaaataaga
     3961 aattaaagta acaagcaaac tataatcttt ttgatgttca attaagtcta actgctttac
     4021 aatttcttct ggagatacta agtcttgttt agcaatttgc ctagaaaggt tattaacagc
     4081 ttcaagtcta cctaaatttg ctgttacacc atctgtattg catactttag cttcttgttt
     4141 accatagcgg ttaatcccgg aagcgataat actgctacta accacataag cttcaaaatt
     4201 ttctatctga agagccttag caaaatgctt cattgtgtct tcgacacgat agacttctgc
     4261 tccgctttca atcagtaatt taccagctct aagaacaacg ctcatatcat tttgcatctt
     4321 atttggctct gccttctact ttattaccct agccacttaa ctaagttggt taaagtccca
     4381 aacatcatcc atccatcctt cataaaagtc cggttcatga cataccatca agattgatcc
     4441 cttataagct tttaaagcac gttttaactc atctttagca tctacatcta aatgattggt
     4501 tggttcatca agtacaagga cattattttc gcgattcatt aataagcaaa atctgacctt
     4561 tgattgctcc ccaccagata acacctgaat ctgactttca atatgctttg aagttaaacc
     4621 acaacgagca agagctgccc taacctctgc ttggttcaaa gcaggaaaag catcccatac
     4681 tgcttcaagt ggagtttggc gattccctcc gggtacttct tgttcaaaat aacctaaatc
     4741 aataaaatca cctcgttcaa cattaccaga aatagggggg ataataccta gtaaactctt
     4801 aagtaatgtt gtttttccta tcccatttgc tccaacaata gctatttttt gattacgttc
     4861 aaatgttagg tttagaggtt ttgttaaagc acgatcatat ccaatttgaa gatctttagc
     4921 ttggaaaata aaacgtcctg gtgtgcgaga ttctttaaat tcaaaacttg gctttggttt
     4981 ctctgcttgt aactcaataa tatccatttt gtctagtttc ttttgacgag acattgccat
     5041 attacgtgtt gcaacgcgtg ctttattacg attaacaaaa tcttgtaaat cagctatttc
     5101 cttttgttga cgttcatagg ctgcttctaa ttgagccttt ttcattgcat agactgattc
     5161 aaaattagta taatcaccgg aatatcttac taaatcttga ttttcaacat gatagacaat
     5221 attgataaca tcgttcaaaa atggaatatc gtgtgagatt aaaacaaaag cattttcata
     5281 attttgtaaa tagcgtttta accaatcaat gtgttcagca tcaaggtagt ttgtaggctc
     5341 atcaagcagg aggatatctg gcttttctaa taacagtttt gctaaaagga cctttgtcct
     5401 ttgaccacca gataattcag taacatcaga atccatacca aaatccataa ctcctaatgc
     5461 ccgtgcgact tcatcaattt tagcatcaag cgtataaaaa tcacgtgatt ctaaacgatc
     5521 ttgtagttct ccaacttctt ccataaggct atctacatct gcgccatcat ctgccatgga
     5581 tatatatata tcattgatac gtgcctctgt tttaaaaagt tcatcaaatg ctgttcgcaa
     5641 aacatcacga actgtttgac cttcttctaa cacagtatgt tgatcaaggt aaccagcagt
     5701 tacatatttt gaccattcaa tttttccttc atcaggttga agatgacctg taacaattga
     5761 catgaatgtt gattttcctt caccattagc accaataagt cctatatgtt caccttttaa
     5821 aaggcgaaaa gaaacattct caaaaatagc acgatcacca aaaccgtgac ttaagttttt
     5881 tacttctaaa atactcattt aaaatccttt gattgataat agaggttatt aaaaaactca
     5941 tagagacact ctacaagttt ttaaattagc acattacata gtaagttcca tatacaagta
     6001 tagagagttt aaacttaaaa gtcaaacctt acttaatttt ttcaaagtgt aaatgaacag
     6061 tgatttgatc atcataacca tttcgctcta aatcacgttg caaacggtat gaaatatagt
     6121 gttcagcaat atgaatgtat cctgcatcca ataaacgatt ttcaaccaaa gactgaatca
     6181 tattgatcgt tggtcgttca acctgagcat cctctaaatc taagattact tttttagtca
     6241 cttgagcaag gtcttgacga agtgactcat caagcacata aactgtttga gcagctttca
     6301 agatagcttg gtagattttt tcaggatcaa attcaacaat ctgtccatca cgtttgatga
     6361 tttgcataag agcccctcct taatgtggaa acaatgttaa aacgatttgc aatcattttc
     6421 atttgttaat ttcattatgc aaatgttttc ataatttgtc aagtgtttta cctgatattg
     6481 atgaaaaatt aatatcacta gaagtcagta taagtattaa gcaatgtcat attcttctat
     6541 attaattcaa attttaactc tttcgctggt ttaaccagcc cattcttatg caatgtttct
     6601 gcgatttgaa cagaattcca agctgccccc ttcagtagat tatctgaaac tacccacata
     6661 tgaacaccat tttcttgatc aaggtctttt cgaatacgac caacaaaggt ctccctatgg
     6721 cctacagcat taatcgcttg agggtaaatt tgctgagatg gaaggtcttg taggacggca
     6781 ccaggaaaat ttgcaatagc ctttttaatt tcagagattg aggctaattc tttcgtctca
     6841 atataaatag actcagaatg acccgataaa acgggaatgc ggacgcaggt agctgaaacc
     6901 ttaatagtag catcttccat aattttcttt gtctccaaag tcattttcat ttcttcgtac
     6961 gtataatcat tatcagtaaa aatatcaatt tgaggtaatg cattgaatgc gataggataa
     7021 tgtttttgat cgctagaaca tggcaagacc gttgcaatta attgatcagg tgaaaggtta
     7081 tcatttaaaa cttgtctcaa ctgttcctta gtttcttcaa cagcacgtgc acctgaaccc
     7141 gaaacagctt gataggtgga aactataaca cgctctatcc cccatttttg acgaatgggc
     7201 tctaaagcaa tcatcatttg aatagtagaa caattgggac aagctatgat accattatga
     7261 ccaatcatgg catgagcatt tacttcagga acaactagtg gaacatcagg attctgacga
     7321 aaatatgacg tgttatctac tacaactgct ccagacttta ctgcataagg agcgaacttt
     7381 gctgaaatag atccaccagc tgaaaacaag gcaatatcaa catcgtaaaa tgattctttt
     7441 gttgtctctt caacacgtat agcctcatct ttaaaatgta aaattttacc tgctgagcga
     7501 cttgatgata aaagtttcac ttgttctatt ggtaaattcg attgttctaa ttgacgaatc
     7561 atttgtgttc ctacggcacc tgtagcaccc acgatagcaa cggtatatcc catataatga
     7621 cctctatttg ttgatgttat tcatcaatat tttcaaaaca ttttaatgtg actattatac
     7681 tacattttta taaactttca agaatagcca aatgaaaaaa gtcaccctaa ccaacctaat
     7741 tataaaaagg aatctcgt
//
LOCUS       DATRUK010000072         7754 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.072, whole genome shotgun sequence.
ACCESSION   DATRUK010000072 DATRUK010000000
VERSION     DATRUK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.072"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            207..677
                     /locus_tag="VBE34_001719"
     CDS             207..677
                     /locus_tag="VBE34_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001889031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO7264419.1"
                     /translation="MVYFRKQFYRVTWDDIDFERNSIYTYRRYSSIKNDFTKPKTRTS
                     IRRVSVSHDLKELLLELKFQQERMLKGLHIKNTDQLIFYDYRFGMISNDAVNKFLASS
                     LEKLEIESKMSSTGARHTYGSYLLANGVDIWAVAKLMGHKDIKQLIETYGHLLL"
     gene            complement(813..883)
                     /locus_tag="VBE34_001720"
     tRNA            complement(813..883)
                     /locus_tag="VBE34_001720"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(849..851),aa:Thr,seq:ggt)
     gene            complement(954..1788)
                     /locus_tag="VBE34_001721"
                     /pseudo
     CDS             complement(954..1788)
                     /locus_tag="VBE34_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018434.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            2099..2353
                     /locus_tag="VBE34_001722"
     CDS             2099..2353
                     /locus_tag="VBE34_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEO7264420.1"
                     /translation="MSYEQEFLKDFEEWLQSQISINQMAMDSAKKVLEEDKDERAADA
                     YIRYESKLDAYRFLQGKFNNYHNQKSFHDLPDGLFGQRHY"
     gene            2462..2773
                     /locus_tag="VBE34_001723"
     CDS             2462..2773
                     /locus_tag="VBE34_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264421.1"
                     /translation="MAKRIKPNKKRELLKAKGINWKDVRLSQTLQEVASPKKKAPEAP
                     KAVDLLEGLTANKQDVLKEAGLVSLEAFAKVSEADVLALKGIGPAAIKQLVDNGAVFA
                     K"
     gene            3041..3610
                     /locus_tag="VBE34_001724"
     CDS             3041..3610
                     /locus_tag="VBE34_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7264422.1"
                     /translation="MQILEDFDGKALPKLETDRLILRQRTVGDVPAMFDYVCLEEVAY
                     PAGLSPIASLEDEYDYFENRYYQNLEKAKLPSGYGITVKGSDRIIGSCDFNHRHEDDV
                     FEIGYMLHPDYWGHGYMTEAVAALIEVGFTLLNLHKIEIRCYDYNKQSRRVAEKLGFT
                     LEATIRDRKDNQGNRCVNLIYGLLRSEWE"
     gene            3708..4292
                     /locus_tag="VBE34_001725"
     CDS             3708..4292
                     /locus_tag="VBE34_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7264423.1"
                     /translation="MIKILGEIVDNQLPVVETNRLLLRQRKLEDAKEIFEFVKLDEVT
                     YPAGFPAVKSLEEEITYIQEIYPTNLEKEKLPSGYAITLKGDDKVIGSVDFNHRHEDD
                     IFEIGYLLHPDYWGQGIVPEAASVLVEIGFTLLGLHKIELGCYDYNKQSQAVARKLGF
                     TLEANIRDRRDAQGQRCGDMRFGLLRSEWEKKRR"
     gene            4289..4855
                     /locus_tag="VBE34_001726"
     CDS             4289..4855
                     /locus_tag="VBE34_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO7264424.1"
                     /translation="MNIIIIGAQASGKMTIGQEIAKQTGMTLFLNHDSIDFVLRFMPW
                     SPDSIALTESIRFKFFETFAKTGQEMIFTIVIDFNDSRDVAFLEKIQTVFQSHNQEVL
                     FVELETELSERLKRNRTENHLKHKPSKRDIKWSENDICSTMDYAIFNPEVAPEALTYY
                     YKINNTCLTATETAYLIIQKINQIKEKN"
     gene            4855..7506
                     /locus_tag="VBE34_001727"
     CDS             4855..7506
                     /locus_tag="VBE34_001727"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEO7264425.1"
                     /translation="MSKELSPKYNPAEVEAGRYQTWLDQDVFKPSGDAEAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQG
                     ISRYDLGREKFLDKVWEWKDEYAATIKSQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYNKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGQNVILPIINKPIPIVADEHADPEFGTGVVKIT
                     PAHDPNDFAVGQRHNLPQVNVMNDDGTMNELADEFNGMDRFEARKAVVAKLESLGNLV
                     KIEKMTHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FMSWMENVHDWVISRQLWWGHQIPAWYNVNGEMYVGEDAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPDTEAADFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFSNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDQARENVEKVVNSQVGNVTDRWILHNLNETVGKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDEKVVTRSVLLYTLDQI
                     LRLLHPIMPFVTEEIFGQYADSSIVTAAYPTVNPAFENAAAHKGVESLKDLIRSVRNS
                     RAEVNVAPSKPITILVKTSDSELESFFKDNSNYIKRFTNPETLEISSAIATPELAMSS
                     VITGAEIFLPLADLLNVEEELARLEKELAKWQKELDMVGKKLGNERFLANAKPEVVQK
                     EKDKQADYQAKYDATQERIVEMQKLVK"
BASE COUNT         2498 a         1325 c         1576 g         2355 t
ORIGIN      
        1 ggagttattt gctcgtattc agaaataata ttatgttatg atatcataaa gagctaaatt
       61 catggtttga tttttatttc tttgaaagtt actaacgatc gtacttattt ctttattgtt
      121 caaattggaa taatctttaa gtagatggaa aagtcgtatt tctaaacatt cctttgaaat
      181 ttctaaatca atggttattt ctttgaatag tttattttcg taaacaattt tatagagtaa
      241 cttgggatga tattgatttt gaaagaaata gtatttatac atatcgaaga tattcttcaa
      301 taaaaaatga ctttacaaag cctaaaacaa ggacttcaat aaggagagtt tcagtaagtc
      361 atgatttaaa agagttattg cttgaactaa aatttcaaca agaaagaatg ctgaaaggtt
      421 tgcatataaa aaatacagat caattgattt tttatgacta taggtttggt atgatatcaa
      481 atgatgctgt taacaaattc ttggcaagct cattggaaaa attagaaata gaatctaaga
      541 tgtcatcaac aggtgctaga catacttatg gaagttattt gttggctaac ggagtagata
      601 tttgggcggt tgcaaaatta atggggcata aagatatcaa acaattaata gaaacatatg
      661 gtcatttgct tctgtaagtg gaagaaaagg aaaatgatca gcttttaaaa attcttaata
      721 ataaaggtag tttttaaaat gactatcaag aacaaattaa gaacaaaaat aaaagaaacc
      781 ctattataat aaggtttcac tcatatattt tctgccacca ccgagaattg aactcggaat
      841 tgagcattac cattgctcta ttataccatt taactatagc ggctactttt ctatgatact
      901 ataagatttt gacgaaatca agtatttctt tacttagaaa aatagtcgtt tttttagtct
      961 aattctattt ggagaaattt actattttca atagctctta aatggtgtgt ttcttgtgga
     1021 gagaggctaa taaggctatc tttttcaagg atatgtttct gatcaccaag gataatttca
     1081 agttttcttt ccaaaacatt tattaatttt ggatgagggc atcgttcatt tttcagtccc
     1141 cggctgatga ttttaatggt attgaataaa ccttcatctt taatcattcc taacggggac
     1201 ataagactta tttttccaaa ttgatattct gaactttaaa aaccattttg taagaataaa
     1261 ttttgccaat catctatttt tagaggagca acgttaacat tcatagtatg tctaagttga
     1321 tcgatcagct tggcagtgtc ttcatcttga taagaaacat catgtgtgag taaacgtcca
     1381 ccaggtttta ggacacgtaa gtactctttt atggctttct ccttagctga attgttaagc
     1441 atcgttaaca ttgcttcgtt gataacgata aactattatc aggaaagggc agtctgagtg
     1501 catttccctg aataacttca ataaattctt ctaattgggc ctttttgaca ttttctttgg
     1561 ccttttctat tactttacta tctagatcaa gcgctgtaat gtgacaggaa aatgctttag
     1621 cgatatcgat ggaactggta cattgattac aagctacttc tagaacacga ctgtcagcac
     1681 taaagtgtcc ttttttgata agccaattcg ttgcttggac acctcctggt cttaagcgtt
     1741 ttttcctagt cgtgctaaga attgatgtcc aatttcttta tgtggcaata agatcacctc
     1801 tttcttttaa tagtaatatc attttttctt aattataaca catagatgct aagataacaa
     1861 ggagtaatag tgattgaatg gtataagtga tgaattttag agagcgtttt cttttaacta
     1921 ggggtgtgat gccaaaacta gaatggcata gagaaccata catctcaaaa ggtcttcctt
     1981 agataaaact agttgttttt atatctgtca gaaaaatcat aatttatttt tgatataatg
     2041 attttttgag tatttaattt atatgttata atgaaaaaaa ctaaaaaaga ggaatatcat
     2101 gtcatacgaa caagaatttt taaaagactt tgaagagtgg ttacaatcgc aaataagtat
     2161 caaccaaatg gcaatggaca gtgcaaagaa agttttagag gaagataagg atgagcgagc
     2221 agcagatgcg tatattcgtt atgagagtaa gttagacgct tatcgctttt tgcaagggaa
     2281 atttaataac tatcataacc aaaaatcttt ccatgatttg cctgatggtc tttttggaca
     2341 acgtcattat taaaaggttt atcgctagaa agcttgtcaa ataggcattt ctatgataca
     2401 ttgttatagt agtttttaga tactatgata gatttcataa atgaggagaa agcattatac
     2461 aatggcaaag cgtattaaac cgaataaaaa aagagagctt ttgaaagcta aagggatcaa
     2521 ttggaaggat gttcgactat ctcaaacttt acaagaagtc gcatcaccga aaaaaaaggc
     2581 tcctgaagcg ccaaaggcag tcgatctttt agaaggttta actgctaata aacaagatgt
     2641 tttaaaagaa gctggccttg tcagccttga agcgtttgcg aaagtaagtg aagcagatgt
     2701 tttagcacta aaaggtattg gtccagcagc tattaaacag ttagttgata atggtgctgt
     2761 ttttgcaaaa tgatttgatt caccttgcga aagtagtctt attttgctag aataaattaa
     2821 cttagaggtg ttttgaaatc ccatttgtca caggaagcgg tcatactgag aaatccgcac
     2881 aaatgtcaaa gcttgttgct aagtccaata attgaaacaa aacgagttgc ggatgttatc
     2941 atccgaattt gggtggtacc gcggaaatcg taatacgaaa ttcgtccctg tcagaattga
     3001 cagggatttt ttgtatttag gagtgaggag attgaagtat atgcagattc tagaagattt
     3061 cgatggcaaa gcattaccaa aactagaaac ggatcgactt atcttgcgtc agcgaacggt
     3121 gggggatgtc ccagccatgt ttgattacgt ttgcttggaa gaggttgcct atccagcagg
     3181 gctcagtcca atagcttctt tagaagatga gtatgattat tttgaaaatc gttactatca
     3241 aaatctggaa aaggcaaaat taccatctgg ttatggtatt accgttaaag gaagtgaccg
     3301 gattatcggc tcttgcgact ttaaccaccg ccatgaagac gatgttttcg agattggcta
     3361 catgcttcat cctgactatt ggggtcatgg ttatatgaca gaagctgtcg cagccttgat
     3421 tgaagtcggt tttaccctcc tcaatctcca caaaatcgaa atccgctgct atgattacaa
     3481 taagcaaagc cggcgcgtgg cagaaaaact aggcttcacc cttgaagcca ccatccgtga
     3541 tcgtaaggat aatcaaggta atcgctgtgt caatttgata tatggtttgc tgaggagtga
     3601 gtgggagtaa ttattctact cttcatagaa gttactagat agactaattt ggctgtgcag
     3661 ggtacttagc taactcatca ataatatcgt agatatggag gagacaaatg attaagatac
     3721 ttggagaaat cgtagataac cagctacctg ttgtggaaac gaatcgtctg ttgttgcgtc
     3781 aacgcaagtt agaagatgct aaggagatat ttgaatttgt caaacttgat gaggtgactt
     3841 atccagcagg ttttccggct gttaaaagct tggaagaaga gataacctat attcaagaga
     3901 tttatccaac taatcttgaa aaagaaaagt taccctctgg ttatgctatt actctaaaag
     3961 gcgatgataa ggtaattggt tcagtagatt ttaaccatcg ccacgaagac gacatttttg
     4021 aaattggcta cctgctccac cctgattatt ggggacaagg cattgtgcca gaagcagctt
     4081 cagtccttgt ggagataggt ttcacactgc taggactaca taagattgag ctgggatgct
     4141 atgactataa taagcaaagt caggcggttg cgcgcaaatt aggttttact ttagaggcta
     4201 atattcgtga tcgaagagat gcccaggggc aacgttgtgg cgatatgcgc tttggtttac
     4261 tgcgaagcga gtgggagaaa aaaagaagat gaatattatt atcatcggag cacaagcttc
     4321 tgggaaaatg acaattggtc aggaaatcgc aaaacagaca ggcatgacct tattccttaa
     4381 ccatgattct atagattttg ttttacgctt catgccatgg tctccagata gtattgctct
     4441 aacggagtct attcgcttta aattttttga aacgtttgct aagactggtc aagaaatgat
     4501 ttttactatt gtaattgatt tcaatgattc aagagatgta gcatttctag aaaagattca
     4561 aacagttttt caatctcaca accaagaggt attatttgta gaattggaaa cagagctaag
     4621 tgagcgtctc aaacgaaatc gcacagaaaa tcacctcaag cataagcctt caaaacgtga
     4681 tatcaaatgg tcagagaacg atatttgttc aaccatggat tacgctatct ttaatcctga
     4741 agtagcccca gaagcgctta catattatta taaaatcaat aatacttgct taactgctac
     4801 tgaaacagca tatcttatta ttcaaaaaat aaatcaaatc aaggagaaaa actaatgtca
     4861 aaagaactat caccaaaata caatcctgcc gaggttgagg caggccgtta tcaaacttgg
     4921 cttgaccaag atgttttcaa gccatcaggg gatgctgagg ctaagcctta ttctattgtg
     4981 attccaccac caaacgttac gggtaaattg cacttgggtc acgcttggga tacaactcta
     5041 caagatatca ttattcgtca aaagcgtatg cagggctttg atacactctg gcttccaggt
     5101 atggaccacg ctggtatagc cactcaagct aaagttgagg agcgccttcg tgagcaaggc
     5161 atttcacgtt atgatcttgg tcgtgaaaaa ttcctagata aagtttggga atggaaagac
     5221 gagtatgcag caactattaa atctcagtgg gggaaaatgg gtctttcggt tgattatagc
     5281 cgtgagcgtt ttacccttga tgagggactt tcaaaagcgg taagaaaagt ctttgttgat
     5341 ttgtacaata aaggttggat ctatcgtggg gagttcatca ttaactggga tccagcagct
     5401 cgtacagccc tttcagatat tgaagttatt cacaaggatg ttgagggtgc tttctatcat
     5461 atgaattata tgctggaaga tggctcacgc gccctagaag tagcaacaac tcgtcctgag
     5521 actatgtttg gagatgtggc tgtagcggta aatccagaag atccacgtta caaggatttg
     5581 attggtcaaa atgttatcct tcctatcatc aataaaccta ttccaatcgt cgctgatgaa
     5641 cacgctgatc cagaatttgg aacaggtgtg gttaagatta cgccggctca cgaccctaat
     5701 gacttcgctg taggtcaacg ccacaactta ccacaagtta acgttatgaa cgatgatggg
     5761 actatgaatg aacttgcgga tgaatttaat ggtatggatc gtttcgaagc tcgtaaggca
     5821 gtagtcgcta aattagaatc gttaggaaac ttagtaaaaa ttgaaaaaat gactcattct
     5881 gttggtcatt cagaacgaac aggcgttgtg gttgaaccac gtttatctac tcaatggttc
     5941 gtcaagatgg atcaattggc taagaatgcc attgccaacc aagatacaga agacaaggta
     6001 gagttttatc caccacgatt taacgatact tttatgagct ggatggaaaa tgtccatgac
     6061 tgggttatct ctcgtcaatt atggtgggga caccaaattc ctgcatggta caacgtcaat
     6121 ggtgagatgt atgttggaga agatgcccca gagggtgacg gatggacaca ggatgaagac
     6181 gttctagaca catggtttag ttctgcttta tggccgtttt caacaatggg atggccagac
     6241 accgaagcag ctgacttcaa acgctacttc ccaacgtcaa ccttggtaac tggatacgat
     6301 atcatcttct tctgggtatc gcgcatgatt ttccaatctc ttgaatttac aggacgccaa
     6361 ccattctcaa atgttcttat tcatggactc atccgtgatg aagaaggacg taaaatgtct
     6421 aaatcacttg gcaatgggat tgatccgatg gatgtcattg agaaatatgg tgcggatgct
     6481 cttcgttggt tcctctcaaa tggttcagcc cctggtcaag atgtgcgttt ttcatacgaa
     6541 aaaatggatg cttcgtggaa tttcattaat aagatttgga acatttcacg ctatatcctt
     6601 atgaataacg aaggtttaac gttggatcaa gccagagaaa atgtggaaaa agttgttaat
     6661 agtcaagttg gaaatgtaac agaccgttgg attctccaca atttaaatga gactgtgggt
     6721 aaagttactg aaaactttga caagtttgaa tttggtgttg ctggtcacat cctttacaac
     6781 tttatctggg aagagtttgc taattggtac gttgagttaa caaaagaggt tctttatagt
     6841 gacaatgaag atgagaaagt tgttacacgt tctgttcttc tttatacctt agatcaaatc
     6901 ttgcgtcttc ttcatccaat catgccattc gtgacagaag aaatctttgg tcagtatgca
     6961 gatagctcaa tcgtaacagc tgcttaccct acggttaacc cagcctttga aaatgctgct
     7021 gcccacaagg gagttgaaag cttgaaggac ttgattcgtt ccgtgcgtaa tagccgagca
     7081 gaggtcaatg ttgctccatc taaaccaatc actatcttgg ttaagacaag tgacagcgaa
     7141 ctcgaaagct tcttcaaaga caatagtaac tacatcaaac gtttcacaaa cccagaaaca
     7201 cttgaaatca gctcagctat tgccacacca gaacttgcca tgtcatcagt catcacagga
     7261 gctgaaatct tcctcccact tgctgacctc ctcaacgtag aagaagagtt agcacgactt
     7321 gaaaaagaac tagccaaatg gcagaaagaa ctggacatgg taggcaaaaa actcggcaac
     7381 gaacgcttcc tcgccaacgc caaaccagaa gttgtccaaa aagaaaaaga caaacaagcc
     7441 gactaccaag ctaaatacga cgcaacccaa gaacggattg tagagatgca aaaacttgtt
     7501 aaatagttaa aatcatccat tacttccgca gagtcctgcc gctagaccat cagtctaagc
     7561 gacagggctt tttgctatat tcaaagattg tgaaaaatta atatatatga tgcctacttc
     7621 ttgcgtctga aatacaagaa gtgaaaatga tttggccata aagaaaaaca ggcagaaacc
     7681 caaatgatta atgcctattt cttatatttt ttcgtcaatt attttataaa taaaattgag
     7741 gtacaaacca gttt
//
LOCUS       DATRUK010000073         7697 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.073, whole genome shotgun sequence.
ACCESSION   DATRUK010000073 DATRUK010000000
VERSION     DATRUK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7697
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.073"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..203
                     /gene="essA"
                     /locus_tag="VBE34_001728"
     CDS             <1..203
                     /gene="essA"
                     /locus_tag="VBE34_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HEO7264426.1"
                     /translation="SDRKKRIKQLFSEESNQFKFVTRTEEATVSADIGHMIMYLVLIS
                     LVLILASIMTFKIKDKKDEYTY"
     gene            187..429
                     /locus_tag="VBE34_001729"
     CDS             187..429
                     /locus_tag="VBE34_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HEO7264427.1"
                     /translation="MNTHINITLDMGETSKDLRIPLKISVKQLVMELDSIFSRTERRT
                     KYQLRVINKGILLGENDILAHHPVTTGDRIRIEEFY"
     gene            429..1703
                     /gene="essB"
                     /locus_tag="VBE34_001730"
     CDS             429..1703
                     /gene="essB"
                     /locus_tag="VBE34_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HEO7264428.1"
                     /translation="METDSFVFEEQDFQFEKGPDFWQLSLKRSKVGTQNLQQLQLLEI
                     HHSLLMPMTTAINADTIQFQFQTEAHALSFESFKKETLSEKLRLALNVLDLDKALSLP
                     VNFILHPSNLFLTKNATAKIAYRSLPGIMTPEKFGPEEFLYQFKCFVFALLTQHDYIE
                     LYNGAISVIEVSDFLKSIYYAETIQAVRDIITTDYEQQVEVETHTLAKVSKAKYKLYK
                     YISVWLGALSTILLIPLVYLVFIHNPFKEKMLAADTSFIKVDYNQVINRLEHVKVSKL
                     PYTQKYELAYSYINGMSFSEEQREVILNNVTLKTDELYLDYWINIGRGLDDDAIDAAK
                     RLDDSDLVIYAIVQKMDQVRKDNSLSGKDREQKLSELQTDYDKYWKDRKTALTDEESK
                     SKNSNNHSTNSNKESSESSSTTASTSSKTKSR"
     gene            1710..6139
                     /gene="essC"
                     /locus_tag="VBE34_001731"
                     /pseudo
     CDS             1710..6139
                     /gene="essC"
                     /locus_tag="VBE34_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
     gene            6144..6860
                     /locus_tag="VBE34_001732"
     CDS             6144..6860
                     /locus_tag="VBE34_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264429.1"
                     /translation="MKKQRLLLLFGGLLIMIMMTACKDSKIPENRTKKEYQAEQNFKS
                     YFKYISDKNNYLDNIKVYYFSISISKDVQDKVSETTTCSYRLEKQKNQEFIGNFEHEV
                     SESSQYSTEVKNQIQYPIQYKDNSIRFTEKTPSERYDEFVFSSFDSSLLKKYKIYDYL
                     LKHPETELKGVSYKIPINSEIVAPFIKQLNIKNPKKSSISVTKTESKEYYYTISIDTD
                     SEIYSIFEGITLEDVKEFSK"
     gene            6857..7387
                     /locus_tag="VBE34_001733"
     CDS             6857..7387
                     /locus_tag="VBE34_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264430.1"
                     /translation="MSPSEKLDRRIDSSSDLSQYVYDFEKQYTEYMKKGYSEKNASIQ
                     ARRTLNEILQENNPGLRSSDAMYDKETGIAAIAVKDKNTGETYIAYAGTNKDADGATD
                     FISDANIGANNSAYLKRLGDKATSFYDRVQSNGDNITVTTGHSYGDFLASRVAIELIP
                     FFINFRKVNDSFHFCY"
     gene            7479..7697
                     /locus_tag="VBE34_001734"
                     /pseudo
     CDS             7479..7697
                     /locus_tag="VBE34_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395449.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2666 a         1308 c         1382 g         2341 t
ORIGIN      
        1 aatctgatag gaaaaaacgt atcaaacaat tattttctga agaaagtaat caatttaaat
       61 ttgttacaag aacagaagaa gcgactgttt cggctgatat tggtcatatg attatgtacc
      121 tggttctcat tagtttggta ctaatattag cttctataat gacttttaaa ataaaggaca
      181 aaaaggatga atacacatat taacattact ctagatatgg gggaaacctc aaaagattta
      241 cgtatccctt tgaaaattag cgttaagcaa ttagtaatgg aattagatag tatttttagc
      301 cgtactgaaa ggcgtactaa atatcaacta agagttataa ataaaggtat tttattggga
      361 gagaacgata tattagcgca ccatccagta acaactggtg atcgtataag gatagaggag
      421 ttttattaat ggaaacagat tcgtttgtat ttgaagaaca agactttcaa tttgaaaaag
      481 gaccagattt ttggcagtta tctcttaagc gttcaaaagt aggaactcaa aatctgcaac
      541 aactgcaatt gttagaaatt caccattctt tgttgatgcc tatgacaact gcaattaatg
      601 cagataccat tcaatttcaa ttccagacag aagcgcacgc tctatctttt gaaagcttta
      661 aaaaagaaac tttatctgaa aaattacgat tagccttgaa tgtcttggat ttagataagg
      721 ctttgtcttt gccagtaaat ttcattttgc atccctctaa tctattttta acaaaaaatg
      781 caactgctaa aatagcttat cgtagtcttc ctgggattat gacacctgag aagtttggtc
      841 cagaggagtt tttatatcaa ttcaaatgtt ttgtctttgc attactgacg caacatgact
      901 atatagaatt gtataatggt gctatttctg ttattgaagt atcagatttc ctaaaaagca
      961 tttattatgc agaaactatt caggctgtta gagacatcat tactactgat tatgagcagc
     1021 aagtagaagt agaaactcat acgttagcaa aagtatcaaa ggcaaaatat aagctttata
     1081 aatacataag tgtctggcta ggagctttat ctacaatact cttgattccg ctagtatatc
     1141 tggtttttat tcacaatcca ttcaaagaaa aaatgttggc agcggatact tcatttatta
     1201 aagttgatta taatcaagtg attaatcgac tagaacatgt aaaagtaagt aagttacctt
     1261 atacacagaa gtacgagttg gcctattcct atattaatgg aatgtcattt tctgaagaac
     1321 agcgtgaagt tattttaaac aatgttacgc tcaagactga tgagctctat cttgattatt
     1381 ggattaatat tggccgtggt ttagatgatg atgctattga tgctgccaaa cgtttagacg
     1441 attctgacct tgttatttat gctattgttc agaaaatgga tcaggttaga aaagacaata
     1501 gtttatctgg taaagatcgt gaacaaaaac tttctgagct acaaacagac tatgataaat
     1561 attggaaaga ccgtaagaca gccttaacag atgaggaatc caaatcgaaa aatagcaata
     1621 atcattcgac aaattccaac aaagagtcat ctgaatcatc aagtactaca gcaagtacat
     1681 cttctaaaac taaaagtagg taggtggata tgttagttat ttgttattat caatcactcc
     1741 gctacgaatt taatcttgaa gaggagaaat cctttctaat atcatcaaac ggcaagtcgc
     1801 caataccagt atcagattta gaaaatgaca tcacgctaaa aaatatgcaa ggtcaattgg
     1861 tatatattat tgaccaaaaa gaaaaagagt taactaatgg tgtagagatt tcaggaattg
     1921 tcttctattt agcgaataat caaaaagaaa tctatactcc cttagattac gaagatatat
     1981 taatcggtga taaagaaggg tatcatgtcc gttttaaaga aggagcccct aatttattac
     2041 tgaaaaaaat agaaagcaat tggcagctta atttatttga aggtgatatt tatctgaata
     2101 atcatcttca aaaagtagtg cagcagttac ctttaagttt aggtgatgag attagcttcc
     2161 aaggaacaat tgttaagctt ttcccagatg aaattcaaat ctgggggggg ggactgatta
     2221 tgaaacatca ttaactaaga aagttatgtc agcttatcaa ttttacgaag gctatcctga
     2281 ttttcaccgc tcacctcgta ttatttatcg ttccagtgaa gataaaatta cagttaatgc
     2341 gcctggtaat aaacctaata agtctaaaga tgaactgtta aaattaattg ttcctcctct
     2401 agtgatgatt ggagtcagta ttcttatttc tatttttcgt cctcgtggta tctatatcat
     2461 tgcaacaatg tcaatggctc tagtgacgat gattttctct atcactggtt actttaaaaa
     2521 tcgtaaacag tataagcagg acttacaaga acgcattgat tcctatcatg actatttatc
     2581 tgataagtca attgaacttc agaagttagc taaagagcaa aagcgtgggc aacactatca
     2641 ttacccaacg attgaaggtt tacaggaaat ggctgatact tatcaccatc gtatttatga
     2701 aaagacacca cttcattttg acttcttgta ttatcgccta ggtttgggag aagtaccaac
     2761 gagctataat attcattatt ctcaaccaga acgttctggg aaaaaagatc ctttagagaa
     2821 cgaaggctat aacctctact ttaacaatcg ttatattaaa aatatgccga ttgttgcgaa
     2881 tttatcacat ggaccagttg gctatatagg accacgaggt ttagtattgg agcaactcca
     2941 attgatggtt aatcaattag cttttttcca ttcttaccat gatgttcaat ttattactat
     3001 tgttccagag gaagagatgg ataagtggtc ttggatgcgt tggttgccac atgcaacctt
     3061 acaagatgtc aatgttcgtg gctttgttta taatcaaaga agccgtgatc aagtgctcaa
     3121 cagtcttaac caaattttaa aattacgtcg cacacaacgc gaagataaat ctgccaaaga
     3181 agggacttta ttttctccgc attatgtcgt tattgtaact gatgaaaagt taattttaga
     3241 tcacgtcatc atggaatttt ttacggaaga tcctacagaa ttgggatgtt ctctcatctt
     3301 tgttcaagat gttatgagtt cactttcaga aaatatcaaa accattatta atattaaaga
     3361 ccgtaatact ggtcagttag ttatagaaga aggagagctt aaagaaacag attttgaatt
     3421 agatcatttc ttagaggatt atgacaagga aaatatttca cgtcgcctag ctccgttaaa
     3481 tcacctacaa aatcttaaat cttcaatacc agaagcagtt acttttatgg aaatgtatca
     3541 agcagaagaa tttgaagatt tgcatgttca agagcgttgg ataagccatg ctccgtataa
     3601 aagcttagct gttccacttg gcttacgtgg tcaggatgat attgtttatc taaacctaca
     3661 tgaaaaagct catggtcctc atggattagt tgcaggaaca acgggatcag ggaagtcaga
     3721 aattatccag tcctatatcc tttctttagc tgttaatttc catccacacg atgtagcttt
     3781 ccttctcata gactacaaag gtgggggaat ggcaaatctc tttaaggacc tgccacactt
     3841 acttggcacc atcaccaact tagatggtgc ccaatcgatg cgtgctttgg tttctattaa
     3901 tgctgaattg aaacgtcgtc aacgtttatt tgctaaagct gatgttaatc atattaacca
     3961 gtatcagaag aaatacaaac ttggagaagt ttcagaacca atgcctcatc tcttcttaat
     4021 ctccgatgag tttgccgaat tgaaatccaa ccaacctgag tttatgaaag agcttgtctc
     4081 aacagcgcga attggtcgtt ccctagggat tcacttaatc ctagcgaccc aaaaaccatc
     4141 tggcgtggta gatgaccaaa tctggtcaaa ctcacgcttc aaactagctc ttaaagtagc
     4201 agaccgcggc gactccatgg aaatgctcca taccccagat gccgccgaga ttacacaagc
     4261 aggacgtgcc tacctccaag ttggtaataa tgaagtctat gaactcttcc aatcagcatg
     4321 gtcaggagca gattaccaac ctgaaaaaga cgaccaaggg attgaagacc ataccattta
     4381 ttccatcaac gaccttggcc aatatgaaat cctaaatgat gatctttcag gcttagacca
     4441 agccgaaaat attaaagaag tacctacgga attagatgcc atcgttgaaa acattcaaac
     4501 tttaaccaaa gaaatgggca tctcagactt acctcaaccg tggctaccgc ctctaagcaa
     4561 tcagattgca gtaaccgatc tccgtaagga agaatcagta gatctctgga gtaaagcccc
     4621 atcttacaaa gttgttctgg gctttatgga tataccaagc caacaagcac aagaagtagc
     4681 ttaccacgat tttgaagatg atgggcattt gtcaattttt gcaggtccaa gtatggggaa
     4741 atccacagcc cttcaaactg taacattgga cttagcacgt cacaatagtc cagaattttt
     4801 aaacctttat ctctttgatt ttggtaccaa tggcctctta ccattaagac gtttgccaca
     4861 tgtagcagat ttctttacca ttgatgatga cgaaaaaata gccaagttta tcagcagaat
     4921 caaaacggaa atgtctgacc gtaaaaaagc cctcagtcgc tacaatgttg caacagcaaa
     4981 actttaccgc caggtatctg gggaaaccat gcctcaaatc ctgattgtta ttgacagtta
     5041 tgaagggcta cgggaagctc aaacactgac taacttagaa gcttgtttcc aaaatatttc
     5101 gcgtgatggg tcgtcacttg gcatttcctt agttatctca gctggtcgta tggcagcttt
     5161 gaggtcgtcc ttaatggcaa accttaaaga acgtatcgct cttaaattaa cagatgactc
     5221 tgaatcacgg acacttgtcg gtcgtcacca acacatcatg gaagatattc caggacgcgg
     5281 attaatcaaa cgtgatgaca ttgaagtgtt acaagtggct ctcccaacag aaggcactga
     5341 aacctttgac atcatcaata acatccaaaa cgaatctgac gccatgaaca gcaaatggac
     5401 tggaccaaga ccaaaagcaa taccaattgt tccagaaaca cttacctttg aggagtttaa
     5461 ggcatatgag gatgttaaaa aagcaagtga gactaatcag atttcacttg gtattagctt
     5521 aaaagaagtt gataattgta gcattccact ttcgaaattc aaacagttac tttttacttc
     5581 tgacaatcca gataatttgt ctaacttgac gcgcttctta gttaaatctg tttcattgat
     5641 taaagatagt aaggtttatc ttttagacac tgcttctgat tatgatgatt tgacagaaca
     5701 acaaattgaa tatatcaata atcgaagtga aatagcagga catttggaag ctattgtttc
     5761 agaaatctat ttaagagaag aatctggtaa aatagaacca tggtttgtta ttattcctga
     5821 tatgaatagt tttgtggcag aaacaaatat ggataaagaa cagattttat tcttacttga
     5881 ctcggctcac aaatatcata tttatttctt atttggtggc ttagcatcat accttatgac
     5941 taatatctca gatgttccaa aagctattag aacacaggca cagtatgttc tcttgggaat
     6001 gagagttatg gatcagtcgg tattaccgaa aacatataat tcaaaagaac cttatttgaa
     6061 accagatatg atttatattc atgatagaag acaagagaca atgcttaaaa tcactcaaga
     6121 aatagaaatg gagcattgag tatatgaaaa aacaaagact attactgctt tttggaggct
     6181 tattaataat gataatgatg acagcatgta aggattcaaa aatcccagaa aaccgcacga
     6241 aaaaggaata ccaggcagaa cagaatttta agtcatactt taaatatata tcagataaaa
     6301 ataactattt agataatata aaagtttatt acttttctat aagtatttct aaagatgtac
     6361 aagataaagt cagtgaaaca acaacttgtt catatagact agaaaagcaa aagaatcaag
     6421 agttcattgg taattttgaa catgaagtta gtgaatctag tcaatattca accgaagtta
     6481 aaaatcaaat acagtatcca atccagtata aagataattc aattcgtttt actgaaaaaa
     6541 caccgtcaga acgttatgat gagtttgttt ttagttcatt tgattcttca ttattaaaaa
     6601 aatataaaat atatgattac ttactaaaac atcccgaaac tgaattaaaa ggtgtttcct
     6661 ataagattcc tataaattct gaaattgtag ccccttttat aaagcaatta aatataaaaa
     6721 atcctaaaaa atcatctatt tcggttacaa aaacggaaag taaagaatat tattatacaa
     6781 tcagtattga tactgattct gagatatatt ctatattcga aggtattaca ttagaggatg
     6841 taaaggagtt ttctaaatga gtccaagtga gaaattagat agaagaattg attcttcatc
     6901 agacctctcc cagtatgtat atgattttga aaagcaatat acagaatata tgaagaaggg
     6961 gtattctgaa aaaaatgcga gtatacaagc acgacgaacc ctgaatgaaa tactacaaga
     7021 aaataatcca ggattgagat ctagcgacgc catgtatgat aaagaaacag gtattgcagc
     7081 tattgctgta aaagataaaa atactggtga gacttatata gcttatgcgg gtactaataa
     7141 ggatgctgat ggggcaaccg actttatttc agatgcaaat atcggtgcta ataactctgc
     7201 ttatttaaaa cgattaggag acaaagctac tagcttttat gatcgtgttc aaagtaatgg
     7261 cgataatatt actgtcacaa caggtcattc ttatggtgac tttctcgctt ctagagtggc
     7321 aattgagcta attccattct ttattaactt tagaaaagtt aatgattcat ttcacttttg
     7381 ttattagctt gtgatcactt ataaagtgac agctagttca tgaaatttct taggagtttt
     7441 ctggctttaa aggtaaccta tgatttaaaa aacatctcat ggaattcaat gcggaatcct
     7501 caacagtctt ttgtggacta acagaagttc aggatgtcgt ttcggcaggc cttaactatt
     7561 ctcagaaggt tttggaaatt tcaatggtac atttgatacc tcaggtaagg gattagaatg
     7621 gacaaaaaca attcaaacag gtatgaaaaa acgagaagag aaaatcaaat accctcattt
     7681 tacaattgtt gacataa
//
LOCUS       DATRUK010000074         7422 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.074, whole genome shotgun sequence.
ACCESSION   DATRUK010000074 DATRUK010000000
VERSION     DATRUK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7422
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.074"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            15..2648
                     /locus_tag="VBE34_001735"
     CDS             15..2648
                     /locus_tag="VBE34_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO7264431.1"
                     /translation="MKGQKIIALAGLVLSCHFALTACHTQEHKNSYHIKTKQVAKKKA
                     NKKKVSVKESHKKRKGVAGVDFPTDDGFLLTKDSKILSHTDSGIVVAHGNHSHFIFYS
                     DLKGSKFSYLIPNANAKTNKNQAVRNFKAGAVAVNTLNDGYVFNPADIVSEDANGYVV
                     RHGDHFHYIPKASLSQQKQVQASRAVSRLGNQSNSHYRVNSSKIAGLHYPTSDGFLFN
                     GRGIKGTTPTGILVEHHNHLHFISFADLRKGGWGSIADRYQPQKKADSKKQSPSSKKP
                     RNENTLPKDIKDKLAYLARELHLDISRIRVLKTLNGEIGFEYPHDDHTHVIMAKDIDL
                     SKPIPNPHHDDEDHHKGHHHDESDHKHEEHEHTKSNKLSDENQKKLIYLAEKLGLNPN
                     QIEVLTSEDGSIIFKYPHDDHSHTIASKDIEIGKPIPDGHHDHSHAKDKVGMATLKQI
                     GFDDEIIQDILHADAPTPFPSNETNPEKMRQWLATVTKINIGQRTNPFQRFGLSLMPN
                     IEVLGIGFTPINDMTPVLQFKKLKQLWMTNTGITDYSFLDKMPLLEGLDISQNGIKDL
                     SFLTKYKQLSLIAAANNGITSLKPLAELPNLQFLVLSHNNISDLTPLSNLTKLQELHL
                     DHNNVKNLSALSGKKDLKVLDLSNNKSADLSTLKTTSLETLLLNETNTSNLSFLKQNP
                     KVSNLTINNAKLSSLDGIEESDEIVKVEAEGNQIKSLVLKNKQGSLKFLNVTNNQLTS
                     LEGVNNYTSLETLSVSRNKLKSLDIKTPNKTVTNLDFSHNNVPTSQLKLNEKNIPEAV
                     AKNFPAVVEGSMVGNGSLAEKAAMASKEDKQVSDNTNHQKNTEKSAQANADSKKENPK
                     THDEHHDHEETDHAHVGHHHH"
     gene            2748..3407
                     /locus_tag="VBE34_001736"
     CDS             2748..3407
                     /locus_tag="VBE34_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO7264432.1"
                     /translation="MTMVKREDVILNATENMVYHKLKNDPSGHDWFHIVRVRNLAVEL
                     AHKEGANTFICQMAALLHDIIDDKICQDSKQASYELTQWLYSQDLAIAEVEHILDILE
                     NISFKAGTGLTMKTLEGQIVQDADRLDAMGAIGIARTMAYSGSKGRLIHDPNLKPREN
                     LTLEEYRNGQDTAIMHFYEKLLKLKDLMNTKQGKMLAQKRHDFLELYLAEFYAEWNGK
                     G"
     gene            3416..3880
                     /locus_tag="VBE34_001737"
     CDS             3416..3880
                     /locus_tag="VBE34_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7264433.1"
                     /translation="MIRRAKEKDLPAIAELLKQILVLHHGVRPDIFHTRGSKFSKEQL
                     KEMLMDESRPIFVYESDEGKVVAHLFLQLQEKRDLPRKSFKTLYIDDLCIDEEVRGQQ
                     IGQKLMDFARQYAKKHGCYNITLNVWNDNQRAVSFYEKLGFKPQQTQMEEIL"
     gene            3880..4317
                     /gene="msrB"
                     /locus_tag="VBE34_001738"
     CDS             3880..4317
                     /gene="msrB"
                     /locus_tag="VBE34_001738"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEO7264434.1"
                     /translation="MKETQEELRQRIGHTAYQVTQNSATEHAFTGKYDDFFEEGIYVD
                     IVSGEVLFSSLDKFQPGCGWPAFSKPIENRMVTNHQDHSHGMHRIEVRSRQADSHLGH
                     VFNDGPVDAGGLRYCINSAALDFIPYDQMAKRGYGDYLSLFDE"
     gene            4466..7258
                     /locus_tag="VBE34_001739"
     CDS             4466..7258
                     /locus_tag="VBE34_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEO7264435.1"
                     /translation="MASTNNVSDTNTRFAKEELEEVFEELGTTQGGLSDEEVAVRQKK
                     YGLNLLSEVKQESIILLFLKNFTSLMAILLWVGGFVAIVSNSLELGLAIWMVNVINGI
                     FSFIQEYRASQATQALKKMLPSYSRVLRKSSEEKVLSEQLVPGDIVLIEEGDCISADG
                     RLIKTTDLQVNQSALTGESNPIYKDNNVENYQSKTLIECDNMVFAGTTVSSGSATMVV
                     TAIGMQTQFGQIADLTQGMKSEKSPLQRELDRLTKQISIISITVGIIFFLAATFFVKE
                     PVSKSFIFALGMIVAFIPEGLLPTVTLSLAMAVQRMAKEHALVKKLSSVETLGATSVI
                     CSDKTGTLTQNEMTVNHLWQNGKSYQVTGLGYAPEGQILFEGDNICFGNSDRGDLEKL
                     IRFAHLCSNAQVLPPNDDRSTYTVLGDPTEACLNVLLEKSGINIQENRKFAPRLKELP
                     FDSVRKRMTTIHSLGGDEKDKKISITKGAPKEILDLSDYVLSDGKVIPLNKEERNKIQ
                     LANDTFAKDGLRVLAVSYCDIEGFSKEQWTQENLEQHMVFIGLIAMSDPPREGVREAI
                     DKCHAASIRIIMVTGDYGLTALSIAKNIGIIRNDDAKVISGLELSEMTDSQLKKELSG
                     EVVFARVAPEQKYRVVTILQEMGEVVAVTGDGVNDAPALKKSDIGVAMGVTGTDVAKE
                     SADMILTDDHFASIVHAVEEGRAVYQNIKKFLTYIFNSNTPEAVPSAFFLFSKGFIPL
                     PLTVMQILAVDLGTDMLPALGLGVEPPETDVMNRPPRRLTDRLLDKGLLIKSFLWYGT
                     IESVLAMGGFFWAHYLRYGNFTFFVANGIPYREATTMTLGAIIFSQIGMVMNSRTSYQ
                     SIKTLSIFGNKLINFGIIMEILAFLVLVYVPLFHNLFNTASLGLSHWLYLISCPFIMI
                     GLDEVRKLFSSRKNKR"
BASE COUNT         2567 a         1175 c         1425 g         2255 t
ORIGIN      
        1 tttatggagg actcatgaaa ggtcaaaaaa ttattgctct agctggtctt gtcctgtcat
       61 gccattttgc tcttacggca tgtcatactc aagaacacaa aaattcttat catattaaaa
      121 caaagcaggt tgctaagaaa aaagctaata aaaagaaagt ctctgttaag gaaagtcata
      181 aaaaacgcaa gggtgttgca ggggttgact ttccaactga tgatggcttt ttactgacta
      241 aagattcgaa gattttatct catacagatt ctggaatcgt tgtagcacat ggaaatcatt
      301 ctcattttat tttttactct gatttaaaag gttcaaaatt tagctattta atccctaatg
      361 ctaatgcaaa aactaataaa aatcaagcgg taagaaactt taaagcaggg gcggtagctg
      421 ttaatacatt aaatgatggc tatgttttta atcccgcaga tattgtttca gaagatgcta
      481 atggctacgt ggtcagacat ggtgaccatt tccactatat tccaaaagca agtttatctc
      541 agcaaaagca agtacaagca agtagagctg tttcacgttt aggaaatcaa agtaatagcc
      601 attacagggt taatagttct aaaattgcag ggcttcacta cccaacaagt gatggtttct
      661 tatttaatgg acgaggaata aaaggcacta ccccaacagg tattttagta gaacatcata
      721 atcatctgca ttttattagt tttgctgatt taagaaaagg ggggtgggga tcaattgctg
      781 accgttacca accacaaaag aaagctgatt ctaaaaaaca gtcaccatca agtaaaaagc
      841 caagaaatga aaatacttta cctaaggaca tcaaagataa gctagcttac cttgctcgtg
      901 aattacattt agatatttca cgtatcagag tccttaaaac actaaatggt gaaattggat
      961 ttgaataccc acatgatgat catacacatg ttattatggc caaagatatt gatctaagta
     1021 agccaattcc aaatccacat catgatgacg aagatcatca caaaggtcat caccatgatg
     1081 agtccgacca taagcatgaa gaacacgagc acactaaatc aaataaatta tctgatgaaa
     1141 atcaaaagaa attaatttat ttagcagaga agcttggttt aaatcctaat caaattgaag
     1201 tattaacgtc agaggatgga agtatcatct ttaagtatcc acatgatgac cactcacaca
     1261 ctatagcaag taaggatatc gaaattggga agcctatccc agatggacac cacgatcact
     1321 ctcatgcaaa agataaagtt ggtatggcaa ctctaaaaca aattggtttt gatgatgaga
     1381 ttattcagga tattctgcat gcagatgctc caacaccatt cccatcaaat gaaactaatc
     1441 ctgaaaaaat gcgtcagtgg cttgcaactg ttactaagat taacattgga caaagaacca
     1501 atccattcca gcgttttggt ctatcattga tgcctaatat tgaagttcta ggaattgggt
     1561 ttactccgat taatgatatg acgccagttt tgcaattcaa aaaacttaaa cagctttgga
     1621 tgacaaatac tggtattaca gattattctt tccttgataa gatgccacta ttagaaggat
     1681 tggatatttc acaaaacggt attaaggatt tatctttcct tacaaaatat aaacaattaa
     1741 gtcttattgc tgcagctaat aatggcatta cttcgctaaa accattggct gaattaccaa
     1801 accttcaatt cttagttttg agtcataaca atatctctga cttgacaccg ttatcaaact
     1861 tgacaaaact ccaagaattg caccttgatc ataataatgt gaagaattta agtgcacttt
     1921 ctggtaaaaa agatttgaaa gttctagatt tatcaaacaa taaatccgca gacttatcga
     1981 ctctaaaaac aactagtctt gagacgcttc ttttaaatga gacgaataca agcaatctaa
     2041 gtttcttaaa gcaaaatcct aaggtatcta atttaacaat taataatgcg aaattatcat
     2101 cattagatgg tattgaagag agcgatgaaa ttgttaaagt agaagctgaa gggaaccaaa
     2161 ttaagtcact agtattgaaa aataaacaag gttcgttaaa attcttgaat gtgactaata
     2221 atcagttaac atcacttgaa ggtgttaata attatacttc acttgagacc ttaagtgttt
     2281 ctagaaataa acttaaatct ttagatatta aaacacctaa caaaacagtt acaaatcttg
     2341 attttagcca taacaatgtt ccaacatcac aattgaaatt gaatgaaaaa aatattcctg
     2401 aagcagtagc taagaatttt ccagcagttg tagaaggttc aatggttgga aatggtagtc
     2461 ttgctgaaaa agcagctatg gcttctaaag aggacaaaca agtttcagat aatactaatc
     2521 accaaaaaaa cactgaaaaa tctgctcaag cgaatgctga tagtaaaaaa gaaaacccta
     2581 aaacacatga tgaacatcat gaccatgaag aaacagatca tgcacatgta ggtcatcatc
     2641 accattaatc ttttacggag ctaatatata aaatccttca agaatttctt ggaggatttt
     2701 gcatttatat gctaaatcat ttattttttt agaaaattag ctataatatg actatggtta
     2761 aaagagaaga tgtgatttta aatgcaactg aaaatatggt ttaccataaa ttaaaaaatg
     2821 atccaagtgg ccatgactgg tttcatattg ttagagtaag aaatcttgca gtagaattag
     2881 cacacaaaga aggtgctaat acctttattt gtcaaatggc tgcacttttg catgatatta
     2941 ttgatgacaa aatctgccaa gatagtaagc aagcgtcgta tgaattgact caatggttat
     3001 atagtcaaga tcttgccata gcagaagtag aacatatcct tgatattctt gaaaatattt
     3061 cattcaaagc aggaactgga ttgactatga aaacattgga aggtcaaatt gttcaagatg
     3121 cagatcgcct agatgctatg ggggctattg gtattgcaag aacgatggct tattcaggga
     3181 gtaaaggacg tttaattcat gaccctaact taaaaccacg agaaaacctc actttagaag
     3241 agtatcgtaa tggtcaggat acagctatca tgcattttta cgaaaaatta ttaaagttaa
     3301 aagaccttat gaatacaaaa caagggaaaa tgcttgctca aaaacgtcat gattttttag
     3361 aactatattt agccgaattt tatgctgaat ggaatggtaa aggttaggag tagatatgat
     3421 tcgtagagcg aaagaaaaag atttgcctgc tattgcagag ctattgaaac aaattttagt
     3481 gttacatcat ggggttcgcc cagatatttt tcatacaaga ggaagtaagt tttctaaaga
     3541 acaattgaaa gaaatgctga tggacgaatc aaggcctatc tttgtttatg aatcagacga
     3601 gggtaaagtt gttgcccact tgtttcttca attacaagaa aaacgtgact tacctcgaaa
     3661 gtcctttaaa actctctata tagatgattt gtgcattgat gaggaagtaa gaggtcagca
     3721 aattggacaa aagttgatgg attttgcaag acaatatgct aagaaacatg ggtgttataa
     3781 tataacttta aatgtttgga atgataatca gagagcggtc tctttttatg aaaaattagg
     3841 ttttaaacct caacaaactc aaatggagga gatactataa tgaaagaaac acaagaagaa
     3901 ttacgtcagc gtatcggaca tacagcttat caagtaaccc aaaattcagc tacggaacat
     3961 gcttttactg ggaagtatga tgattttttt gaagagggga tatatgtaga tatcgtcagt
     4021 ggagaggttt tattctcttc tttagataaa tttcaacctg gttgtggttg gccagcattt
     4081 tcaaaaccaa ttgagaatcg aatggtgaca aatcatcaag atcactctca tggaatgcat
     4141 cgtattgagg ttaggagtcg tcaagctgac tctcatttag ggcatgtatt taatgatgga
     4201 ccggttgatg caggtggctt acgctattgt atcaattcag ctgccttaga ttttattcct
     4261 tatgaccaga tggcaaagag aggctatggg gactacttat cactttttga tgaataagag
     4321 acgacttaag ggtatatctt aagtcgtttt ttatgtatgg taaaaagaat tggtggcagg
     4381 tgtattcttc atcagtttat tttctgaaaa aagagtataa tataggtatc aaaaatgaaa
     4441 acgttttaga gaaggagact agaatatggc ttctacaaat aatgtttcag atacaaacac
     4501 acgttttgct aaagaggaat tagaagaggt tttcgaagaa ttagggacaa ctcaaggtgg
     4561 tttatcagat gaagaggttg ctgtacgtca gaaaaaatat ggtttaaacc tgttatctga
     4621 agtaaagcaa gaatcaatta ttttattgtt tctgaaaaat tttactagtc ttatggcaat
     4681 actattatgg gttggaggat ttgtggctat tgtttctaat agcttagagc taggtcttgc
     4741 catttggatg gttaatgtga ttaatggtat ttttagtttc attcaggaat accgtgcaag
     4801 ccaagcaaca caagctctca agaaaatgtt accatcttat tcacgtgttc ttagaaaaag
     4861 ctcagaagaa aaggtattat cagagcaact agtcccagga gatattgttc ttattgaaga
     4921 aggagattgt atttcagcag atggtcgctt gattaaaaca acagatttgc aagtaaacca
     4981 atcagctttg acaggggaat caaatcctat ttataaagat aacaatgttg aaaactacca
     5041 gtctaaaaca ttgattgaat gtgataatat ggttttcgca ggaacgacag tctccagtgg
     5101 tagcgcaaca atggttgtta cggcaattgg aatgcaaacc caatttggtc aaatcgctga
     5161 tttaacacaa gggatgaaat cagaaaaaag ccctcttcaa agagaacttg atcggttgac
     5221 caaacagatt tctatcattt caattactgt aggaattatc tttttcttag cagcgacttt
     5281 ttttgttaag gagccagtgt caaaatcatt tatctttgcc ttggggatga tcgttgcatt
     5341 tatcccagaa ggtttactac ctaccgttac actttctcta gctatggcag tccaacgaat
     5401 ggctaaagaa cacgcacttg tcaaaaaact ttcttcagtt gagactcttg gagcaacctc
     5461 agttatctgt tctgataaga ctggtacatt aacgcaaaat gaaatgaccg tcaatcattt
     5521 gtggcaaaat ggaaagagtt accaagtgac aggactgggt tatgctccag aaggacagat
     5581 tctttttgaa ggtgacaata tttgctttgg taatagtgat agaggagact tagaaaaact
     5641 tattcgtttt gctcatctct gtagcaacgc tcaagtatta ccaccaaatg atgatcgttc
     5701 aacttatact gtacttggag atccgacaga agcttgtttg aatgttttat tggaaaaatc
     5761 agggataaac atacaagaaa atcgtaagtt tgcaccacgt ttgaaagagt taccttttga
     5821 ttcggttaga aaacgcatga caactattca tagcttagga ggcgatgaaa aggataaaaa
     5881 aatttcaatc acaaaaggtg cgccgaaaga aatacttgat ttatcagatt atgtcttatc
     5941 tgatggcaaa gttatcccct taaataagga agaacgtaat aaaatacagt tggctaatga
     6001 tacatttgct aaagatggac ttcgcgtctt agctgtttca tactgcgata ttgaaggctt
     6061 ttcaaaagaa caatggacgc aagaaaattt ggaacaacac atggtgttta tagggttgat
     6121 agcaatgagt gatccacctc gtgaaggcgt tcgtgaagct attgataaat gtcatgcagc
     6181 aagcattcgt attattatgg tgactggaga ttacggatta acggcactga gtatcgctaa
     6241 aaatattggt attattcgta acgatgatgc taaagtaatt tcaggtttag agctatcaga
     6301 aatgacggat agccaattga aaaaagaatt gtcaggtgaa gtcgtttttg ctcgtgtagc
     6361 accagaacaa aaatatcgag tagtaacgat attacaggag atgggtgaag tagtagcagt
     6421 tacaggagac ggggtcaatg atgcgcctgc gctaaaaaaa tccgatattg gtgtagccat
     6481 gggtgttacc ggtactgatg ttgctaaaga atcagctgat atgattctga cagatgatca
     6541 ttttgcttct attgtacatg ccgtggaaga agggcgggct gtttatcaaa acattaagaa
     6601 attcttgacg tatattttta atagtaatac tcctgaggcg gttccgtcgg cattcttcct
     6661 tttttctaaa ggttttattc ctttaccatt gactgtaatg caaatacttg ctgttgattt
     6721 gggaaccgat atgcttcccg ctttaggtct aggcgtggaa ccaccagaaa cagatgttat
     6781 gaatcgtcct ccaagacggc taacagatcg cttattggac aaagggctac ttataaaatc
     6841 tttcctttgg tacggcacta ttgaatccgt attagctatg ggtggttttt tctgggctca
     6901 ttacttacgt tatggtaatt ttactttttt cgtcgcaaac ggcataccgt acagagaagc
     6961 gacaactatg acacttggag ctattatctt tagccagata ggtatggtta tgaatagcag
     7021 aacgagctat caatctatta aaacactatc tatttttggt aataaattaa ttaattttgg
     7081 tataataatg gagattttag catttttagt cttagtatac gtgccactat tccataactt
     7141 attcaacaca gcaagcctag gtttatcaca ttggctctat ttaataagct gcccgtttat
     7201 catgataggt ttagatgaag ttcggaaatt attcagcagt agaaagaata aacggtgatg
     7261 tcaaatataa taacttattt aaaaaataat tcaaacttaa cttttgatga attagcatta
     7321 aacgatgttg acattctttg tttaaatgaa tttggctata tttcttttga aaaacttata
     7381 aacactacag aaatgaagtc agttttagtc tgtgagttat at
//
LOCUS       DATRUK010000075         7383 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.075, whole genome shotgun sequence.
ACCESSION   DATRUK010000075 DATRUK010000000
VERSION     DATRUK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.075"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            33..1406
                     /locus_tag="VBE34_001740"
     CDS             33..1406
                     /locus_tag="VBE34_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7264436.1"
                     /translation="MLKKLFAPPQKRERLSAEEIKHKYPRYRVQVLISIFVGYMGYYF
                     VRNTTSILSGILNMSATEIGIITCASYIAYGLSKFISGLISDESNSKIFLPVGLFLTG
                     LVNVLIGVIPSVITSIWLFTIMYLINGWLQGMGYPPGARTLVYWYDNKERIKYATIWN
                     LSHNFGGAIAPILTGVGLALAGNDSLNQARAAYWFPGVVACLLAVLVYFLQEDTPESI
                     GLPPIEEYHKEQYTNVVDSSDILEEPEVLGMGEIIKKYILPNTKLMWASLYSIFVYIL
                     RYGIVSWTPKFLATSVQDGGKGITATAGMGGFSLFEIGGIIGMLTAGYLSAKVFKNSK
                     PLTNVAFLVVAILLLAAYWFIPAGPQYMALDFIILLGLGASIYGPVMMVGLYAMELVP
                     KAAAGAASGLTGTFSYVGGATIATLAIGIIIDHFGWGVAFIIFGISGFAAIVCTLLSR
                     DKSLEYW"
     gene            complement(1564..1870)
                     /locus_tag="VBE34_001741"
                     /pseudo
     CDS             complement(1564..1870)
                     /locus_tag="VBE34_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2033..3019
                     /locus_tag="VBE34_001742"
     CDS             2033..3019
                     /locus_tag="VBE34_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEO7264437.1"
                     /translation="MSEKIRVLLYYKYVSIENAEEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWIHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENEAKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPQVANI"
     gene            complement(3056..4183)
                     /locus_tag="VBE34_001743"
     CDS             complement(3056..4183)
                     /locus_tag="VBE34_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEO7264438.1"
                     /translation="MKHYQTIIIGAGAAGIGFGSAMQRLGLTNFLIIEKGHIGESFLR
                     WPRTTQFITPSFTTNGFGFPDLNAVIPDTSPAFSFEKEHLSGVEYARYLQLVAAHYNL
                     PIQNETSVLSIDKRDSLFVIKTSKGDFSADYLIMATGEFQNPNTIDIKGADLGMHYGQ
                     VDNFHIKSDNPFIIIGGNESACDALTHLVYLGNQVELYTDTFGRKESNPDPSISLSPL
                     TKERLKHIQDHKKEYYSISEGKKAIEIKQIGKQYQVTFDDGSTAESFHKPILSTGFLN
                     TCHLIDGIALFEYDKNQLPIVTEDDESTIVNNCFLIGPSLRQRDTIFCYIYKFRQRFV
                     PVINHIAQREGIILDEDTLEFFKENQMYLDNLACCDVNCDC"
     gene            4287..5306
                     /locus_tag="VBE34_001744"
     CDS             4287..5306
                     /locus_tag="VBE34_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEO7264439.1"
                     /translation="MFLLLCILSMWLMIFGVAFYYFGSLHQSLTSRIIYQFVLTVLLT
                     TTAWFMGAYFLAFEGHFKTVFQFQEADGKQIVNCLFQLCFALYAVVMLIGSIIDRVQT
                     KRLLLAVVSWLFLVYTPLAYLIWNSEGVFAKMGVLDFSGGMVVHLSAGLSSYILAHVI
                     GKSEHQHNKVKNDSLFLGMILITFGWFGFNMGPVGEWNSQAIMILLNTIFAIIGGGLA
                     WTLAAKWNGEEEKTGSLLNGIIVGLVTSTAGVGYLLTWQLLAVTFFASLFTYFVTDYV
                     AKAFAIDDVVSSFGMNGIGGLLGSLGVGLFKLSHMPVQLLALATTILLSIIMTYIISK
                     AIFRK"
     gene            5631..6437
                     /locus_tag="VBE34_001745"
     CDS             5631..6437
                     /locus_tag="VBE34_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7264440.1"
                     /translation="MSKKIILGILSLLSVVTLVACGSSDKQLQDKVEKKGKLVLAVSP
                     DYAPFEFKALVNGKDKVVGADIELAQAIADELHVKLEVSPMSFDNVLSSLQTGKADMA
                     ISGLSYTKERAKVYDFSKPYYTTENAVLVRKSDLGKYTSIESLKGKKIAVQKGSIEEG
                     VSKKSFKNSHVVSLTAMGEAISELKSGKVEAVDLEKPVAEGYLAQNPDLVLAKFALKT
                     GEGDAKAVALPKDSGKMLKTVNKVIKRLEKEDKYKVYISDAAKLTGNQVE"
     gene            6520..7023
                     /locus_tag="VBE34_001746"
     CDS             6520..7023
                     /locus_tag="VBE34_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEO7264441.1"
                     /translation="MVQGSINIEKYKDLALQKQGEHRKFLASLKKKAPKNLDKIVQQI
                     HQEVFQEIDCTACANCCKTLGPLFTEADISRISKHFRMKLSAFEDLFLEVDEDGDKVF
                     KAMPCPFLGDDNLCSIYDVRPKACREFPHTDRKKIYQINNLTIKNTLTCPAAYLFVEK
                     LKSRIGK"
     gene            7127..7303
                     /locus_tag="VBE34_001747"
     CDS             7127..7303
                     /locus_tag="VBE34_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEO7264442.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
BASE COUNT         2324 a         1063 c         1485 g         2511 t
ORIGIN      
        1 aacacatcta aaaatcggaa aggtaatttg ctatgttgaa aaaactattt gcaccccctc
       61 agaaaagaga acgtttatca gctgaggaaa ttaagcataa atatcctcgt tatcgtgtac
      121 aagtccttat ttctattttt gtaggttata tgggatacta ttttgttcgt aatacgactt
      181 caattttatc tggtatcctt aatatgtctg ctactgagat tggtattatt acctgtgcta
      241 gctacattgc ttatggtctc tctaaattta tttctggttt gatttcagat gaatcgaatt
      301 ctaaaatttt ccttccagtt ggtttgtttc ttacaggact agttaatgtg ttaatcggtg
      361 ttattccatc tgtaattact agtatctggc tctttactat catgtatctt attaatggtt
      421 ggttacaagg gatgggatac cctcctggag cgcgtacatt agtctattgg tatgataata
      481 aagaaagaat taaatatgct accatttgga atttatctca caattttggt ggagcaatag
      541 cgccgatttt aactggtgta ggtctagctc ttgctggtaa tgattctctt aatcaagcgc
      601 gtgctgctta ttggtttcca ggggtggtgg cttgtctttt ggctgttctt gtttactttt
      661 tacaagaaga tactccagaa tctatcggac ttccgcctat agaagagtat cataaagagc
      721 aatatactaa tgttgtggat tcaagtgaca ttttagaaga gcctgaagtt ttgggtatgg
      781 gcgaaattat aaaaaaatat attctaccaa atactaaact gatgtgggca tccttgtatt
      841 caatatttgt atatatttta cgttacggta ttgtctcatg gactcctaag tttttagcga
      901 cgagtgtgca agatggtgga aaaggtatta ctgcaacagc tggtatgggt ggtttttcac
      961 tttttgaaat aggtggtatt attgggatgc taacagcagg atatctatct gccaaagtgt
     1021 ttaaaaactc taaaccttta accaatgtag ccttcttagt tgtagcaatc ttattactag
     1081 ctgcctattg gtttattcca gcaggacctc aatatatggc attagacttc attattttac
     1141 taggtttggg ggcatcaatc tacggtcctg ttatgatggt tggcctgtat gctatggagt
     1201 tagttccaaa agcagcagct ggagcagctt caggattgac tggcacattt agttatgtcg
     1261 gaggagcaac gatcgctact ctagctattg gtattattat tgaccacttt ggttggggag
     1321 tagcatttat aatatttggt atttctggat ttgcggctat tgtctgtacc ttgctttcaa
     1381 gagataagag tctagagtat tggtgatatt atttggtaaa agtatcattt aatatttagt
     1441 gagataagtt atgaaaaaag gcctcttgaa agagaccttt tttgttttag ataatctatt
     1501 tgttgaatta attagtgata tcgattgttt ttaatagata gtttctacct agttaaaata
     1561 aaactacccc attaataatg attcaatttt aatgtcatat tcagggtagt gtatgcttaa
     1621 atgttcgata atcacatgat aaatttgtgt gatttttgct cttttttctg ggaagtcatg
     1681 accaatatag tggaaggaac attctatcgt taaaaagagc tcatggaata aggtattgac
     1741 ttcttctaaa agttctaaaa gtttttttat cttcagtaat aaacgcaggt gcagtattga
     1801 tttcatcagc ccaagcatct acaggtctaa gtggataagt accgtattct agacataatt
     1861 cataagtcat aaaagtcctc ctagtctttt actattttaa catatgtgtg ttcttaagaa
     1921 gaataatttg tctttaaagc aaagaatatg tcctaagcgt attttttgct atgttgtaag
     1981 gagtcgttat ataatgaagt atactaactt attttgcaga tgaggagaaa aaatgtcaga
     2041 aaaaatcaga gttttactct attataaata tgtttcaatt gaaaatgcgg aagagtacgc
     2101 tgcgaaacat ctagaatttt gtaaatctat tgggcttaaa ggtcgtattt tgattgcgga
     2161 tgaggggatt aatggaactg tttctggaga ttatgaaaca actcagaagt atatggattg
     2221 gatccacagc gatgagcgtt ttgctgatct ttggttcaag attgacgagg aaaatcaaca
     2281 agctttccgt aaaatgtttg ttcgttataa gaaagagatt gtgcaccttg ggttagaaga
     2341 taataacttt gatagcgata ttaatccttt ggaaacaaca ggtgagtatt taaatcctaa
     2401 acaatttaaa gaagccttgc ttgatgaaga tactgttgtt ttagatacgc gtaatgatta
     2461 tgagtatgac ttaggtcact tccgtggagc aattcgtcct gatattcgca atttccgtga
     2521 gttaccacaa tgggtacgtg acaacaaaga taagtttatg gaaaaacgtg ttgttgtgta
     2581 ctgtaccggt ggtgttcgtt gtgagaaatt ctcgggttgg atggttcgag aaggttttaa
     2641 agatgttgga caattacacg gtggtattgc aacatatgga aaagatccgg aagttcaagg
     2701 tgaactttgg gatggtgcga tgtatgtttt tgatgatcgt atctcggtgc caattaatca
     2761 tgttaatcca actgttatta gtaaagatta ttttgatgga acaccttgtg aacgttatgt
     2821 caattgtgca aatccgtttt gtaacaaaca aatttttgct tcagaagaaa acgaagcgaa
     2881 gtatgttcga gggtgctcgc ctgaatgtcg tgcacatgaa cgtaatcgct atgtgcaaga
     2941 aaatggttta tcacgtcaag aatgggcaga acgtttagaa gctattggag aatccttacc
     3001 tcaagtagca aatatttaat attaaaaaat cgctagcaat aaagctagcg attttttagc
     3061 aatcacaatt gacatcgcaa caagctaggt tatcaaggta catttgattt tctttaaaga
     3121 attcaagggt atcttcgtct aatataatcc cttctcgttg tgcgatatga ttaataacag
     3181 ggacaaagcg ctgtctgaat ttataaatat agcagaaaat agtatctctt tgtcgtaagc
     3241 ttggaccaat taagaaacag ttattaacga tagtagattc gtcatcttca gtaactattg
     3301 gcaactgatt tttatcgtat tcaaataaag cgattccgtc aatgagatga caggtattta
     3361 gaaaacctgt ggaaagaata ggtttatgga aagactccgc agtactaccg tcatcaaaag
     3421 taacttgata ttgttttcca atttgtttaa tctcaatggc tttcttgcct tcagagatac
     3481 tataatattc ttttttgtgg tcttgtatat gttttaaacg ttctttagtt aggggggata
     3541 aagagatgct tggatcggga ttgctctctt tacgaccaaa cgtatctgta tataattcga
     3601 cttgatttcc caaatatact agatgtgtta gtgcatcaca agcactttcg tttccaccaa
     3661 tgatgataaa tggattgtca gatttgatat ggaaattatc aacttgacca tagtgcattc
     3721 ctagatctgc acctttaata tcaatggtat ttggattttg aaattctcct gttgccataa
     3781 taagataatc agcagaaaaa tctccctttg aagttttaat aacaaaaagg ctatctcgtt
     3841 tatcaataga tagaacagat gtttcgtttt ggatagggag attataatga gcagcaacta
     3901 attgtaagta gcgtgcatat tccactccag aaagatgttc tttttcaaat gagaaagcag
     3961 gagaagtatc tggaataaca gcgtttagat ctggaaaacc aaagccattg gtagtgaagg
     4021 aaggtgtgat aaattgtgtt gttctgggcc atcttaaaaa agattctcca atgtgccctt
     4081 tttcaatgat aagaaaattt gtaagtccta atcgttgcat ggcagaacca aaaccgattc
     4141 ctgcagcgcc tgcaccgata attatggttt gataatgttt catgtcttct ccttgacaaa
     4201 aataaatgtg taagataaaa tacttaacca gttaacaaca atacagttta acaaataaaa
     4261 gagtgtttgt aaagaaagga ctatttgtgt ttttactatt atgtatttta tcgatgtggc
     4321 tcatgatttt tggggttgca ttctattact ttgggtcgct acatcaatca ttgacttcaa
     4381 ggattattta tcagtttgta cttactgttt tgctaacgac aacagcttgg ttcatgggtg
     4441 cctatttttt agcatttgaa ggtcatttta aaactgtctt tcaattccaa gaagcagatg
     4501 gtaaacaaat tgtaaattgt ttatttcaac tttgtttcgc cctttacgca gtagtaatgc
     4561 ttattgggtc aattattgat agagttcaga cgaagcgact tttattagct gttgtttctt
     4621 ggcttttctt ggtgtatacg cccttagctt atttaatttg gaattcagag ggtgtttttg
     4681 caaagatggg agtacttgat ttttcaggcg gaatggtcgt tcatttatct gcagggcttt
     4741 cttcgtatat tttagctcat gttattggca agagtgaaca tcagcacaat aaggttaaaa
     4801 atgatagtct ctttttagga atgatactta ttacatttgg ttggtttgga tttaatatgg
     4861 gtccagttgg tgagtggaat tcacaagcca ttatgatact tttaaatact atatttgcta
     4921 ttataggtgg tggtttggcg tggacattgg ctgctaagtg gaatggtgag gaagagaaaa
     4981 caggaagctt attgaatggc attattgttg gtcttgtgac gagtacagca ggagttgggt
     5041 atctcttaac ttggcaatta ttagctgtaa cattctttgc atccctcttc acctactttg
     5101 taacggatta cgttgcgaaa gcttttgcca ttgatgatgt cgtttcctcc tttggaatga
     5161 atggtattgg gggattgttg ggaagtttag gagttggtct ctttaaatta tcacatatgc
     5221 cagttcaact gttagcatta gctaccacaa ttcttttgtc aattataatg acctatatca
     5281 tatctaaagc gatttttaga aaataataaa taaggttgag atgtacatct caaccttatt
     5341 tattatttta gcaaggagag tgactaaatc gaattattat cattttctta ctagacagca
     5401 actgtacaag gttctgaaag ttagttgatt attaatgata gttagcattt tttaagaatt
     5461 ggaatacttt taaaaggtga ttgttttaat tagttatagt tgttattaaa aatcgtctaa
     5521 cagcaagcat agttatcggt gtaatttact gaatttttat tcaaaaaaac ttgacttatg
     5581 tataaagatg atttatactt gttcctatca taataaaagg aagatagttt atgtcaaaaa
     5641 aaataatatt aggaatttta tctcttttat ctgtcgttac tttggtggcg tgtggttcat
     5701 cagacaaaca gctacaagat aaagttgaga aaaaagggaa gttagtttta gcggtgagtc
     5761 cagattatgc tccctttgag tttaaagcgc ttgttaatgg gaaagataaa gttgtcggcg
     5821 cagatattga gttagctcaa gctattgctg atgagcttca tgtcaaacta gaggtatctc
     5881 ctatgagttt tgacaatgtt ttatcaagtt tacaaacagg taaggctgat atggctattt
     5941 ctggattatc atatactaaa gagcgtgcta aagtctatga tttttcaaaa ccttattaca
     6001 ctacagaaaa tgcggttctt gttaggaagt ctgatttagg aaaatatact tcaatcgaaa
     6061 gccttaaagg taaaaaaata gcagtacaaa aaggaagtat cgaagaggga gtttctaaga
     6121 aatcattcaa aaattctcac gtcgtgtcac tgacagcgat gggagaagcg attagtgagt
     6181 taaaatcagg caaggtagaa gctgttgatt tagaaaaacc cgtagctgaa ggttacttgg
     6241 cccagaatcc agaccttgtt cttgctaagt ttgcccttaa aacaggagaa ggggatgcta
     6301 aggctgtagc tttacctaaa gacagtggaa aaatgttgaa gactgttaac aaagtgataa
     6361 agagacttga aaaagaagat aaatataaag tttatatttc agatgcggct aaattaactg
     6421 gcaatcaagt tgaataatat taaaaagcct agtcataggc ttttttggat gttataatat
     6481 ggcttgacta agaataatag cataaaggca gaggaatata tggttcaggg ttcaattaat
     6541 attgaaaaat ataaagattt ggcgctccaa aaacaaggcg agcatcgtaa atttttagca
     6601 agtttaaaaa agaaagctcc taaaaactta gataaaatag tccaacagat acatcaagag
     6661 gtttttcaag aaattgattg tacagcatgt gctaattgtt gtaaaacttt gggtcctcta
     6721 tttacagaag ctgatatttc taggatttct aaacatttcc ggatgaagtt atcggctttt
     6781 gaagatttat ttttagaagt agatgaagat ggagataaag tatttaaagc gatgccttgt
     6841 ccgtttttag gcgatgataa cttatgtagt atatacgatg tcagacctaa agcttgtcgt
     6901 gaatttccac atactgatcg taagaaaatc tatcaaatta ataacttaac gataaaaaat
     6961 actcttactt gtccagcggc ataccttttt gtggaaaaat tgaaatcacg tatagggaaa
     7021 taataaccca gagaaagtat cataaaaata ttgacaatct gcttttattt gggtatccta
     7081 gtaagtgaac gtgaaaatgt tacaatatct tgaggaggtg aaacatatgt caaaaacagt
     7141 agtacgtaaa aacgaatcac ttgacgatgc acttcgtcgt ttcaaacgtt ctgtgactaa
     7201 agctggtact cttcaagaat cacgtaaacg tgaattctac gaaaaacctt ctgtaaaacg
     7261 taaacgtaaa tcagaagcag ctcgcaaacg taaaaaattc taattagata gaatatgaaa
     7321 aaacagttca gaattgagct gttttttcat atattataac tataagttaa taaaaaaact
     7381 cgg
//
LOCUS       DATRUK010000076         6944 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.076, whole genome shotgun sequence.
ACCESSION   DATRUK010000076 DATRUK010000000
VERSION     DATRUK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.076"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            62..271
                     /locus_tag="VBE34_001748"
     CDS             62..271
                     /locus_tag="VBE34_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEO7264443.1"
                     /translation="MKVLFDVQNLLKKFGIYVYIGKRLYDIEVMKIELQRLYDNGLIS
                     RDDYLKAELILRREHRLELEKENKK"
     gene            268..1236
                     /locus_tag="VBE34_001749"
     CDS             268..1236
                     /locus_tag="VBE34_001749"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEO7264444.1"
                     /translation="MSKKLLGIDLGGTTIKFGILTLEGEVQEKWAIETNTLENGRHIV
                     SDIVESLKHRLSLYGLTKDDFLGIGMGSPGAVDRTSKTVTGAFNLNWADTQEVGSVIE
                     KEVGIPFFIDNDANVAALGERWVGAGANNPDVVFVTLGTGVGGGVIADGNLIHGVAGA
                     GGEIGHMIVDPENGFTCTCGNKGCLETVASATGVVRVARQLAEQYEGSSAIKAAIDNG
                     DTVTSKDIFIAAEDGDKFANSVVERVSRYLGLAAANISNILNPDSVVIGGGVSAAGEF
                     LRSRVEKYFVTFAFPQVKKSTKIKIAELGNDAGIIGAASLANQQAS"
     gene            1248..1628
                     /locus_tag="VBE34_001750"
     CDS             1248..1628
                     /locus_tag="VBE34_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO7264445.1"
                     /translation="MDMSVILIIVILLAFVAWASWNYWRVRRAAKFLDNESFQKEMSR
                     GQLIDIREAGAFHRKHILGARNIPASQFKVALSALRKDKPVLLYDASRGQSIPRIVLL
                     LRKEGFNQLYVLKDGFNYWTGRVK"
     gene            1860..3701
                     /gene="typA"
                     /locus_tag="VBE34_001751"
     CDS             1860..3701
                     /gene="typA"
                     /locus_tag="VBE34_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEO7264446.1"
                     /translation="MTNLRTDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELEER
                     AMDSNDIEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPSEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSMSDDPSDQEKTMAPIFDTIIDHIPAPVDNSEEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERKEIQEAKAG
                     DLIAVSGMEDIFVGETVTPTDAIEPLPVLRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVQCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGVLVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLADDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            3746..3991
                     /locus_tag="VBE34_001752"
     CDS             3746..3991
                     /locus_tag="VBE34_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEO7264447.1"
                     /translation="MFYLIVALLIALYYFFMAPKTVRNTLNAIGLVGLVALLLVLAVM
                     SFIKILQLPGELYIGLIMIPLGYTAFKETLNLSEKKK"
     gene            4121..5476
                     /locus_tag="VBE34_001753"
     CDS             4121..5476
                     /locus_tag="VBE34_001753"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEO7264448.1"
                     /translation="MKTITTFENKKVLVLGLARSGEAAARLLAKLGAIVTVNDGKPFD
                     ENPTAQSLLEEGIKVVCGSHPLELLDEDFCYMIKNPGIPYNNPMVKKALEKQIPVLTE
                     VELAYLVSESQLIGITGSNGKTTTTTMIAEVLNAGGQRGLLAGNIGFPASEVVQAADD
                     KDILVMELSSFQLMGVKEFRPHIAVITNLMPTHLDYHGSFEDYVAAKWNIQNQMSSSD
                     FLVLNFNQGISKELAKTTKATIVPFSTTEKVDGAYVQDKQLFYKGENIMSVDDIGVPG
                     SHNVENALATIAVAKLAGISNQVIRETLSNFGGVKHRLQSLGKVHGISFYNDSKSTNI
                     LATQKALSGFDNTKVILIAGGLDRGNEFDELIPDITGLKHMVVLGESASRVKRAAQKA
                     GVTYSDALDVRDAVHKAYEVAQQGDVILLSPANASWDMYKNFEVRGDEFIDTFESLRG
                     E"
     gene            5479..6555
                     /locus_tag="VBE34_001754"
     CDS             5479..6555
                     /locus_tag="VBE34_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEO7264449.1"
                     /translation="MGKKIVFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     EQINQSGLDITFHSIATGKLRRYFSWQNMLDVFKVGVGVLQSIAIIAKLRPQALFSKG
                     GFVSVPPVVAARLLKVPVFVHESDLSMGLANKIAYKFATIMYTTFEQSKDLIKTKHIG
                     AVTKVMDCKKSFENTDLTSIKEAFDPNLKTLLFIGGSAGAKVFNDFITQTPELEEKYN
                     VINISGDSSLNRLKKNLYRVDYVTDLYQPLMNLADVVVTRGGSNTIFELVAMKKLHLI
                     IPLGREASRGDQLENAAYFEEKGYALQLPESELNINTLEKQINLLISNSESYEKNMSQ
                     SSEIKSQDEFYQLLIDDMAKVTKG"
     gene            6559..>6944
                     /locus_tag="VBE34_001755"
     CDS             6559..>6944
                     /locus_tag="VBE34_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HEO7264450.1"
                     /translation="MPKKKSDTPEKEEVVLTEWQKRNLEFLKKRKEDEEEQKRINEKL
                     RLDKRSKLNISSPEEPQNTTKIKKLHFPKISKPKIEKKQKKEKIVNSLAKTNRIRTAP
                     IFVVAFLVILVSVFLLTPFSKQKTITV"
BASE COUNT         2303 a         1072 c         1420 g         2149 t
ORIGIN      
        1 caattagcaa gcggttgcgg attttcttga aaattgaatg gttttctgtt aaaattcata
       61 tatgaaagtt ttattcgatg tgcagaatct tttgaaaaaa tttggtattt atgtttatat
      121 tggtaaacgt ctttatgata tcgaagttat gaagattgaa ttgcaacgtc tctacgataa
      181 tggtctgata agtagagatg actatttaaa agcagaactg attttaagaa gagagcacag
      241 actagaattg gagaaggaaa ataaaaaatg agtaagaaat tattgggaat tgacctcgga
      301 ggaacgacca ttaaatttgg tatcttgacg cttgagggag aagtacaaga aaaatgggca
      361 attgagacca atactttaga aaacggaaga catatcgttt ctgatatcgt tgaatctctc
      421 aaacatcgtt tgagcctcta tggattaaca aaagatgact ttctcggtat cggtatgggt
      481 tctccaggag ctgttgatag aactagtaaa acagtaacag gtgcttttaa tctaaattgg
      541 gctgatactc aagaagtagg ttcggttatt gaaaaagaag ttggaattcc attttttatt
      601 gataacgatg ctaatgttgc agcacttggt gaacgctggg taggtgctgg tgccaataat
      661 cccgatgttg ttttcgtaac cctcggaaca ggagtaggtg gaggtgttat cgcagatggt
      721 aacctcatcc atggtgttgc aggagcaggt ggagaaattg ggcatatgat tgttgatcca
      781 gaaaatggat ttacgtgcac atgtggtaac aaaggctgtc ttgagacagt tgcatcagcg
      841 acaggtgttg ttagagtagc acgtcaactc gcagaacaat atgaaggttc gtctgccatt
      901 aaagcagcga ttgacaacgg tgatactgtt acaagtaaag atatttttat agcagcagaa
      961 gatggggata aatttgctaa ttctgttgtt gaacgtgtat cacgttacct tggactggca
     1021 gcagctaata tttcaaatat tttaaaccct gattctgtgg ttattggtgg cggtgtctca
     1081 gcagcaggtg aatttttacg tagtcgcgtt gagaaatact ttgtcacatt tgctttccca
     1141 caagttaaaa agtcaactaa aattaagatt gctgaactag gtaatgatgc tggtattatt
     1201 ggtgcagcaa gcttagccaa tcaacaagca agttaaggag ataagatatg gatatgtctg
     1261 ttattttaat tatcgttatt cttctggctt ttgttgcttg ggcatcttgg aattattgga
     1321 gggttcgtag ggcagctaaa tttttagata acgagtcttt tcaaaaagaa atgtcacgag
     1381 gacagttaat tgatatccga gaagctggtg cttttcatag aaagcatatt ctcggagcac
     1441 gtaacattcc agcaagtcaa tttaaagttg ccttatcggc tttacgcaaa gataaacctg
     1501 tcctcttata tgacgcaagc cgtggtcagt ctattccaag gattgttttg ttactcagaa
     1561 aagaaggttt taatcaactt tacgtgttaa aggacggttt caattactgg acgggtagag
     1621 ttaagtaata tactaaggag agagttacta ttaataactc tcttctcttt ttgtagaaat
     1681 gaattaaatt gaaaaaagta gaaaataaat gacataaata atgaaatgat gaaaagtttt
     1741 cctatcacaa ataggcagtt aatatgaaaa catttacact tgtgtaaatt ctgtttttta
     1801 agaaaaattg tgttataatt cataagttaa cagaattaca ttataaaata gaggaaaaca
     1861 tgacaaattt aagaacagat atccgtaacg ttgcgatcat tgcccacgtt gaccacggta
     1921 aaacaactct cgttgatgaa ttattaaaac aatcacatac tcttgatgag cgtaaagagc
     1981 ttgaagaacg tgcaatggat tcaaatgata tcgagaaaga acgtggtatc accattcttg
     2041 caaaaaatac agccgtagca tacaacgatg ttcgtatcaa tattatggac acacctggtc
     2101 acgcggactt tggtggtgaa gttgagcgta ttatgaaaat ggttgatggt gttgttttag
     2161 tcgttgatgc ttacgaagga acaatgccac aaacacgttt tgttttgaag aaagctcttg
     2221 aacaaaactt aattccaatc gttgttgtaa ataaaattga taagccatca gctcgtccat
     2281 cagaggttgt tgatgaagtt cttgaactct tcattgagct cggtgctgat gatgaccaac
     2341 tagatttccc tgttgtttat gcttcagcca tcaatggaac atcttcaatg tcagatgatc
     2401 cttcagatca agaaaaaaca atggcaccga tttttgatac tatcattgat cacattccag
     2461 ccccagttga caactcggaa gaaccacttc aattccaagt ttctcttctt gattacaatg
     2521 attttgtagg acgtattggt attggacgtg ttttccgcgg gactgtcaaa gttggagatc
     2581 aagttactct ttcaaaactt gatggtacaa ctaaaaactt ccgcgtaaca aaactttttg
     2641 gtttctttgg acttgaacgt aaagaaatcc aagaggctaa agcgggtgat ttaatcgctg
     2701 tttctggtat ggaagatatc ttcgttggtg agacagtaac tccgacagat gctattgaac
     2761 cactaccagt tttacgtatt gacgagccaa cacttcaaat gactttcttg gtgaataatt
     2821 caccatttgc aggtcgcgaa ggtaaatgga ttacgtcacg taaggttgaa gaacgtcttt
     2881 tagcagaatt acaaacagac gtttctttgc gtgttgaccc aacagattcg ccagataaat
     2941 ggacggtttc agggcgtgga gaattacatt tatctatcct cattgaaaca atgcgtcgtg
     3001 agggatatga acttcaagta tcacgtccag aagttatcat caaagaaatt gatggtgttc
     3061 aatgcgagcc gtttgagcgt gttcaaattg atactccaga agaatatcag ggtgctatta
     3121 tccaaagttt gtcagagcgt aaaggtgata tgcttgatat gcagatggtt ggtaatggtc
     3181 aaacgcgttt gattttcttg attcctgcac gtggtttgat tggttattca acagagtttc
     3241 tttcaatgac acgtggatat ggtatcatga atcatacttt tgaccagtat ctaccggttg
     3301 ttcaaggaga aattggtggt cgtcatcgtg gtgtcttggt ttctattgaa aatggtaaag
     3361 caactacata ttcaattatg cgtattgaag aacgtgggac tatctttgta aatccaggta
     3421 cagaagttta tgaaggaatg attgttggtg agaattctcg tgataatgat ctcggagtca
     3481 atattacaac tgctaaacaa atgacaaatg tccgttcagc aactaaagat caaactgcag
     3541 tcatcaagac gcctcgtatc cttactttag aagagtcact tgaattttta gcagatgatg
     3601 agtacatgga agtaactcct gagtctattc gtcttcgtaa acaaatttta aataaagcag
     3661 cacgtgacaa agctaataaa aagaaaaaat cagctgaata atatccaaag tatttcttat
     3721 ttatgattaa taagaaagga gttctatgtt ttacttaatt gttgcccttt tgattgcctt
     3781 atattacttt tttatggcac caaaaacagt acgtaatact ttgaacgcta ttggtttagt
     3841 aggtttggta gccttattac ttgtcttggc agttatgagt tttattaaaa tccttcaatt
     3901 gccaggtgaa ttgtatattg ggcttataat gattcctcta ggatatacag catttaaaga
     3961 aacattgaat ttgtcagaaa agaagaaata gcttatgaac ttaggatttt gtctcctaag
     4021 ttcttttgtt ttgagaaatt agagacatat cctttacctt gaaaaagcca ctgattaaag
     4081 gtaaaataat agggataata atgatagaat gggacgagta atgaaaacaa taacaacatt
     4141 tgaaaataaa aaagttttag tccttggttt agcacgatct ggagaagccg ctgcacgttt
     4201 gttagctaag ttaggagcaa tagtgacagt taatgatggc aaaccatttg atgaaaatcc
     4261 aacagcacag tctttgttgg aagagggtat taaagtggtt tgtggtagtc atcctttaga
     4321 attgttagat gaggattttt gttacatgat taaaaatcca ggaatacctt ataacaatcc
     4381 tatggtcaaa aaagcattag aaaaacaaat ccctgttttg actgaagtgg aattagcata
     4441 cttagtttca gaatctcagc taataggtat tacaggctct aacgggaaaa cgacaacgac
     4501 aacgatgatt gcagaagtct taaatgctgg aggtcagaga ggtttgttag ctgggaatat
     4561 cggctttcct gctagtgaag ttgttcaggc tgcggatgat aaagatattc tagttatgga
     4621 attatcaagt tttcagctaa tgggagttaa ggaatttcgt cctcatattg cagtaattac
     4681 taatttaatg ccaactcatt tagattatca tgggtctttt gaagattatg ttgctgcaaa
     4741 atggaatatc caaaatcaaa tgtcttcatc tgattttttg gtacttaatt ttaatcaagg
     4801 tatttctaaa gagttagcta aaactactaa agcaacaatc gttcctttct ctactacgga
     4861 aaaagttgat ggtgcttacg tacaagacaa gcaacttttc tataaagggg agaatattat
     4921 gtcagtagat gacattggtg tcccaggaag ccataacgta gagaatgctc tagcaactat
     4981 tgcggttgct aaactagctg gtatcagtaa tcaagttatt agagaaactt taagcaattt
     5041 tggaggtgtt aaacaccgct tgcaatcact cggtaaggtt catggtatta gtttctataa
     5101 cgacagcaag tcaactaata tattggcaac tcaaaaagca ttatctggct ttgataatac
     5161 taaagttatc ctaattgcag gaggtcttga tcgcggtaat gagtttgatg aattgatacc
     5221 agatatcact ggacttaaac atatggttgt tttaggggaa tcggcatctc gagtaaaacg
     5281 tgctgcacaa aaagcaggag taacttatag cgatgcttta gatgttagag atgcggtaca
     5341 taaagcttat gaggtggcac aacagggcga tgttatcttg ctaagtcctg caaatgcatc
     5401 atgggacatg tataagaatt tcgaagtccg tggtgatgaa ttcattgata ctttcgaaag
     5461 tcttagagga gagtaactat ggggaaaaaa attgttttta caggtggtgg tacggtaggt
     5521 catgtaacac taaacctaat attaatcccc aaatttatca aagatggttg ggaagtacat
     5581 tatattggtg ataaaaatgg catagaacat gaacaaatta atcagtctgg acttgatatc
     5641 acatttcact ctatagctac tgggaaacta cgtcgctatt tttcatggca aaatatgtta
     5701 gatgtcttta aagtaggggt tggcgttctt caatcgattg ctattattgc taaacttaga
     5761 ccccaagcct tattttcaaa agggggattt gtttcagtac ctcctgtagt agctgcacgc
     5821 ctattaaaag ttcctgtttt tgttcatgaa tcagatttat caatggggtt agcaaataag
     5881 attgcttata aatttgcgac tattatgtat acgacttttg aacaatctaa agatttaatc
     5941 aaaactaagc atattggtgc tgtaacaaaa gtcatggatt gtaaaaagtc gtttgaaaat
     6001 actgacttaa ctagtataaa agaagccttc gatccaaacc taaagacctt actatttatt
     6061 ggaggatcag caggtgcaaa agtattcaat gattttatta ctcaaacacc tgagctggaa
     6121 gaaaaatata atgttatcaa tatttcaggc gattcttctc tcaatcgatt gaagaagaac
     6181 ctttatcgcg tggattatgt tacagacctt tatcagcctt taatgaattt agcagatgtg
     6241 gttgttacac gaggtggctc taatactatt tttgaattag tagctatgaa aaaacttcat
     6301 cttattatcc ctcttggacg tgaagcgagc cgaggtgacc aattagagaa tgctgcttat
     6361 tttgaagaaa agggctacgc attgcagtta ccagaatctg aattaaacat aaatacactt
     6421 gagaaacaga taaatttatt aatttctaac agtgagagtt acgaaaaaaa catgtcacaa
     6481 tcatctgaaa taaaatctca agatgaattt tatcaattat taattgatga tatggcaaaa
     6541 gtaacaaaag gataaaacat gcctaagaag aaatcagata ccccagaaaa agaagaagtt
     6601 gtcttaacgg aatggcaaaa gcgtaacctt gaatttttaa aaaaacgcaa agaagatgaa
     6661 gaagaacaaa aacgtattaa cgaaaaatta cgcttagata aaagaagtaa attaaatatt
     6721 tcttctcctg aagaacctca aaatactact aaaattaaga agcttcattt tccaaagatt
     6781 tcaaaaccta agattgaaaa gaaacagaaa aaagaaaaaa tagtcaacag cttagccaaa
     6841 actaatcgca ttagaactgc acctatattt gtagtagcat tcctagtcat tttagtttcc
     6901 gttttcctac taactccttt tagtaagcaa aaaacaataa cagt
//
LOCUS       DATRUK010000077         6600 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.077, whole genome shotgun sequence.
ACCESSION   DATRUK010000077 DATRUK010000000
VERSION     DATRUK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.077"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            5..1198
                     /locus_tag="VBE34_001756"
     CDS             5..1198
                     /locus_tag="VBE34_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO7264451.1"
                     /translation="MIDFLLLKSLHSLLGLTITVCDQNFSVIREYKSEKTISLFYNHY
                     LILSNFSKTQHDFLFHYGSLGELFLVHHIQQYYIIIGPWRSNVIDPLLLKKKLTETQI
                     NASEQDYFIDRLSQLPFFSLSQIRELLIVTNYCLTGVVKDKLSEPLHYYTKGWSNSFD
                     LDKIKQFSKQNMSSYKYQYHFENNILKAVKSGSEFLLKETVEQFSNSIVPIISGDELR
                     SEKNYSIMIYDRLSQATIQAGLDIETAYRARDRFIKENESTISLNEVLKLRDTAILFY
                     TQQVHSLKKHLETPHSQTIVAVIRYLENNLNRFIKTEEIAKECHMSESKLRKLFKQEK
                     HITIQQYFLNLKIEAAKQLLDENKKVEEVSNLLGFSTSSNFSRTFKKIVGISPLEYKQ
                     KPKTI"
     gene            complement(1393..1917)
                     /locus_tag="VBE34_001757"
     CDS             complement(1393..1917)
                     /locus_tag="VBE34_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO7264452.1"
                     /translation="MVKDRQIQKTKVAIYNAFISLLQENDYSKITVQDVIGLANVGRS
                     TFYSHYESKEVLLKELCEDLFHHLFKQGRDVTFEEYLVHILKHFEQNQDSIATLLLSD
                     DPYFLLRFRSELEHDVYPRLREEYITKVDIPEDFLKQFLLSSFIETLKWWLHQRQKMT
                     VEDLLKYYLTMVER"
     gene            2049..2912
                     /locus_tag="VBE34_001758"
     CDS             2049..2912
                     /locus_tag="VBE34_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO7264453.1"
                     /translation="MTSNKKVAIAFILNISFSVLEFIFGSLFFSGAILADAVHDFGDA
                     IAIGISATLEKKSKKDEDTIFSLGYKRFSLLGALITSLILISGSILVMIENIPKLWHP
                     TPVNYHGMFILAVIAIIINGLASFILHSGQSKHEEILSLHFLEDILGWLAIIVISLIL
                     NWKPLYILDPLLSVAISTFILSKALPKLLSTLKLFLDGVPDSIDYAALHDELKGLSQV
                     RSINQLNIWSMDGIDNRAIIHCCLNQLISEKDCKRAIRTICQHYKINDVTVEIDYSLR
                     EHQNHCKPLKN"
     gene            2997..3851
                     /locus_tag="VBE34_001759"
     CDS             2997..3851
                     /locus_tag="VBE34_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO7264454.1"
                     /translation="MTDTMILNDNHKIPTLGLGTWMIDNDRVAQVVKDAIELGYRHID
                     TAQAYNNEKGVGVGIKESGITRESLFVTTKIAAEHKDYESATKSIDRSLEALGLDYID
                     LMIIHSPQPWKEWRETDKHFDQGNLEAWRALEDAQKSGKVKSIGVSNFLEKDLENILK
                     NGYVKPAINQILAHIGNTPFDLIDYCQSKGIQVEAYSPIAHGQALKSDGIQKMAEKYG
                     VSVAQLCIQYLLQLNLIVLPKASSKEHLQSNLDFDFVISDEDMSILKSLMFDDYGEFS
                     NFPVFSGK"
     gene            3961..4988
                     /locus_tag="VBE34_001760"
                     /pseudo
     CDS             3961..4988
                     /locus_tag="VBE34_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            5004..5393
                     /locus_tag="VBE34_001761"
     CDS             5004..5393
                     /locus_tag="VBE34_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7264455.1"
                     /translation="MHIKEVSKKTGITSDTIRYYERINLIPPIHRSESGIREFTQNDI
                     DILEFVRYFRGAGVSVESLIDYIHLVEKGDSTIPARLEILKDKRNNLEDRLQGLQEAL
                     NRLNHKIDNYQKVLVPREQELFDPTTK"
     gene            5403..5798
                     /locus_tag="VBE34_001762"
     CDS             5403..5798
                     /locus_tag="VBE34_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO7264456.1"
                     /translation="MQETTKSIFPKGEKNPYGEFFIRQSYLAALAKSPDGNVSVGNVT
                     FEAGCRNNWHVHLDGYQILLVTEGSGWYQEENKKAVSLEPGNVIVTDKGVKHWHGAKK
                     DSEFAHIAITAGKSEFYEAVSLEEYSRLG"
     gene            5826..6143
                     /locus_tag="VBE34_001763"
                     /pseudo
     CDS             5826..6143
                     /locus_tag="VBE34_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660666.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            6230..6514
                     /locus_tag="VBE34_001764"
     CDS             6230..6514
                     /locus_tag="VBE34_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HEO7264457.1"
                     /translation="MLTIQEMKDTTFELQENFRRLNYPIKQVAKDLQLNISEVESLLS
                     LDVTYPGDVCMLRDYLEDMLKKEGKEVYPFSRLASHSANRWYPYETPWRY"
BASE COUNT         2268 a         1071 c         1138 g         2123 t
ORIGIN      
        1 tcatatgatt gattttctac tgttgaaatc attacacagt cttctaggtc taactattac
       61 tgtttgtgat caaaattttt ctgttatcag agagtataaa tccgagaaaa ctatttcact
      121 tttttacaat cattacctta ttttaagtaa ctttagtaaa actcaacatg attttttatt
      181 tcattatggc tctttaggag aactttttct tgtccaccat attcaacaat attatattat
      241 catcggtccc tggcgtagca atgttattga ccctttactt cttaagaaaa agctgactga
      301 aacacaaatc aatgctagtg aacaagacta ctttattgat agactatccc aattaccttt
      361 cttctcactt agtcaaatcc gagaattact aatagtaacc aattattgtc taactggggt
      421 tgtgaaagat aaattatcag agcccttaca ttactacacg aaagggtgga gcaactcctt
      481 tgatctagat aaaattaagc aattttctaa acagaatatg agttcttaca aatatcagta
      541 tcattttgaa aataatattc taaaagctgt taaatcaggt agtgaatttc ttttgaaaga
      601 aacagtggaa caatttagca attctatcgt acctataatc agtggagatg aattgcgatc
      661 tgaaaaaaac tactccatca tgatttatga tcgtctctca caggctacca tccaagcagg
      721 acttgatatc gaaacagctt atcgagcacg agatcgtttt ataaaagaaa acgagtcaac
      781 tataagtcta aatgaagttt taaaattacg tgatactgct atcttattct atactcaaca
      841 agttcattct ttaaaaaaac atctcgaaac ccctcattct caaactattg tcgcagtgat
      901 tcgatatctt gaaaataatc taaatcgctt tattaaaaca gaagaaatag ctaaagaatg
      961 tcacatgagt gaatcgaagt tacgaaaact atttaagcaa gaaaaacaca tcactattca
     1021 acaatatttt ctaaacttaa aaatcgaagc tgctaagcaa ttactagatg aaaataagaa
     1081 agtagaagaa gtttccaatt tacttggatt ttccacttct tctaattttt caaggacatt
     1141 taaaaaaata gtgggaatta gcccactaga atataagcaa aagcctaaga caatatagta
     1201 atcgaaaaat attcattttg cttatggtga catcaacaat cccaaagaaa ctctttcatc
     1261 aaggggactt ttttatctcc cgaattatac tcatttaatc taataatttc aactcgctat
     1321 attagacaat caatccattc atcacctctg ataacgctaa tgaaataaga gaatctccca
     1381 actctctata atttacctct ctaccatagt taaataatat tttagtaaat cttcaacagt
     1441 cattttctgt ctttgatgaa gccaccactt taaggtctcg ataaaactgg ataaaagaaa
     1501 ttgttttaag aaatcttcag gaatgtccac ctttgtaata tactcctcac gcaatctagg
     1561 gtaaacatcg tgttctaatt cacttctaaa gcgtaataaa aaatagggat cgtcagataa
     1621 taataacgta gcgatactat cttgattttg ttcaaaatgt tttaagatat gaactaaata
     1681 ctcttcaaac gtaacatctc taccctgctt aaataagtgg tgaaaaaggt cttcacagag
     1741 ctccttgagt aacacttctt tgctttcata gtgagagtaa aaagttgatc ttcctacatt
     1801 tgcaagtccg attacatctt gaaccgttat tttagaataa tcattttcct gtagcaagct
     1861 tataaaagca ttataaatcg ctacttttgt tttctgaatt tgtctatctt ttaccatata
     1921 ggacactttc taagttttgt tcagtattga acaagtgttc ttttttgttt ctagcaattc
     1981 tcttttatct cctagataat agaagaaaga atcacactac aataccagta taacaaaagg
     2041 agaaaaatat gacatcaaac aaaaaagtgg ctatcgcttt tatattaaat atcagctttt
     2101 ccgtccttga attcatcttt ggaagtctat tcttctcagg agctatctta gccgatgctg
     2161 ttcatgattt tggtgatgca atagctattg gcatctcagc tactttagaa aaaaaatcta
     2221 agaaagatga agatacgata ttttcattag gttataaacg ttttagcctt ttaggagctc
     2281 ttatcacaag tctaattctt ataagtggat ctatcttagt catgatagaa aatattccaa
     2341 aattgtggca tcctactcct gttaattatc acggcatgtt tattctagct gtcatcgcta
     2401 ttatcattaa tggtctagct agttttattc tccattctgg tcaaagtaaa catgaggaaa
     2461 tattaagcct tcattttttg gaagatattc tagggtggtt agctattatt gttatatctc
     2521 ttatcttaaa ttggaaaccg ttgtatatcc ttgatccatt attatcagtt gccatatcta
     2581 ctttcatctt atctaaagca cttcctaaat tactatcaac cctgaaactt tttttagatg
     2641 gtgttccaga ttccatagat tacgctgcat tacatgatga acttaaggga ctttcgcaag
     2701 taagatctat caatcaactc aatatctggt caatggatgg tattgataat cgagcaatca
     2761 ttcactgttg cttaaatcaa ttgatatctg aaaaagattg taaaagagct attcgaacga
     2821 tttgccaaca ttataagata aacgatgtta ctgttgaaat cgattattct ttaagagaac
     2881 atcaaaatca ttgcaaacca ttaaaaaact aacctgagtg ttagtttttt ccttgacttg
     2941 gagttaactc taagtgttaa gattaggtca atactaatga aagaaggaaa ataaagatga
     3001 cagatacaat gatattaaat gacaatcata aaataccaac tttaggttta ggtacgtgga
     3061 tgatagataa tgatagggta gctcaagttg ttaaagatgc tatcgaatta ggctaccgcc
     3121 atattgatac tgcacaagca tacaacaatg aaaaaggggt cggtgtaggg attaaagaat
     3181 ctggtattac acgtgaatct ttatttgtta cgactaaaat tgccgctgaa cataaagatt
     3241 atgaaagtgc tacaaaatca attgatcgat cactagaagc tttagggctt gattatattg
     3301 atttaatgat tattcatagt ccacagcctt ggaaggaatg gcgtgaaaca gataaacatt
     3361 ttgaccaagg aaatttagaa gcttggagag ctttagagga tgctcaaaaa tctggtaagg
     3421 taaaatccat cggtgtctca aactttttag aaaaagactt agaaaatatc ttaaaaaatg
     3481 gttatgttaa acctgctatc aatcaaatct tagctcacat cggaaataca cctttcgact
     3541 tgattgatta ctgtcaaagt aaaggtattc aagttgaagc ttattcacca attgcacatg
     3601 gacaagcatt gaaatctgat ggtattcaaa aaatggctga aaaatatggg gttagtgttg
     3661 ctcaattatg tattcaatat ttactacaat taaacctgat cgttttacca aaggcttcta
     3721 gtaaagaaca tttacagtct aatctagact ttgactttgt aattagtgac gaagatatgt
     3781 cgatattaaa atcacttatg tttgacgatt atggggaatt ttctaacttc cctgttttta
     3841 gtggaaaatg aaaccatatg cggattttta cagatgttta attttcaaaa tttttaagtt
     3901 tttcttgact tgaagttaac tctaagagtt ataatgaagt tatagaaaag aggtaaggtt
     3961 atgaaagtag ctacatttat tgaacctggc aaaatggtta taactgatac accaaaacca
     4021 gtcattgaac aagagacaga tgctgttatc aaaattgtta gagcctgtgt ttgtggttca
     4081 gacttgtggt ggtatcgtgg tatttcaaaa cgtgaaagtg gttcttttgc aggtcatgag
     4141 gctattggta tcgttgagga agttggtact aaagtaactg acgtgtcaaa aggtgatttt
     4201 gttattgttc cctttacaca tggctgtggc caatgtccgt cttgtaaggc tggatttgat
     4261 ggaaattgta caaatcatca agctgaaaaa aatgtagata ccaaggtcaa tatctacgtt
     4321 atactaatgc aaactgggca ttggttaaaa ttcctggtca accttctgac tatgataatg
     4381 agacacttaa ctctcttctc accttatcag atgtaatggc aactggttat catgctgcag
     4441 aagttaaaga aggtgatacg gtagttgtca tgggagatgg tgctgttgga ctctgtggtg
     4501 ttattgctgc taaaatgtta ggtgctaacc gtatcattgc aatgagtcgt cacaaagata
     4561 ggcaggaact agcattgact tttggtgcaa cggatattgt cgaagaacgg ggtgacgaag
     4621 ccgttaaacg tgttttagat ttaaccaatc aagcaggtgc cgatgctatt ttagaatgcg
     4681 ttggtacaga gcagtctgtt gatacagcta cccaaattgc taggcctggt gctgtcatag
     4741 gacgtgttgg tatcccacaa aatccagaca tgaataccaa taatttattc tggaaaaata
     4801 tcggccttag aggtggcatt gcttctgtaa caacatttga taagtctgtc cttttagatg
     4861 ctgttctaac tcataaaatt aacccaggct tagtttttac aaaatccttt gtattagatg
     4921 atattcaaaa ggcttatgaa gcaatggata aacgtgacgc tatcaaatcc ttagtgattg
     4981 ttgactgaat atcgatgtta aaaatgcata tcaaagaagt tagtaaaaag actggcatta
     5041 cgtcagatac cattaggtat tatgagagaa ttaatcttat tccccctatt catcggagtg
     5101 aatcaggaat tcgtgagttc acacaaaacg atattgatat tttagaattt gtccgctatt
     5161 ttagaggcgc aggtgtttcc gttgaaagtc tcattgatta tattcatttg gttgaaaaag
     5221 gcgattccac aatcccagct aggctagaaa ttttaaaaga taaacgcaac aaccttgaag
     5281 ataggcttca aggcctccaa gaagcactta atcgtctaaa tcacaaaata gacaattatc
     5341 aaaaagttct tgttcctaga gaacaagaac tttttgatcc aacaacgaaa tgaggaaatc
     5401 ttatgcaaga aacaactaag tctatctttc ctaagggtga aaaaaatcca tatggtgaat
     5461 tctttattag gcaatcctat ctcgcagcat tagcaaaatc tccagatggg aatgtttcag
     5521 ttggaaatgt aacctttgaa gctggctgtc gtaataattg gcatgttcat ttggacggtt
     5581 atcaaatcct cctcgttaca gaaggttcag gttggtacca agaagagaat aaaaaggcag
     5641 tttcattaga accaggtaac gtgattgtaa ctgataaagg cgttaaacat tggcatggtg
     5701 ctaaaaaaga ttctgaattt gcacatattg cgattacagc tggtaagagt gagttttatg
     5761 aagctgttag tcttgaagaa tattcaagac taggttaaat tcaagtaagg aggaatcaat
     5821 ccattatgaa agaaaaacaa acagccggac gtcgtcaatt agaagaattt gcacctgaat
     5881 ttgccagata taatgatgat attttatttg gtgaggtttg ggcaaaagag gatcatttaa
     5941 cagataaaac acgttctatt attactatat ctgccttaat tagctgagga aatcttgaac
     6001 agttagagca tcatcttcaa tttgccaaac aaaacggagt tactaaagaa gaaattgctg
     6061 acattatcac tcacctcgct ttttatgttg gatggccaaa agcttggtca gcctttaata
     6121 aagctaagga aatttggata taaaataaca gacatagcaa gggagatact ttcccaaaag
     6181 ccaaaaagaa agatcaacca attattaaac caactattag gagaatgata tgcttactat
     6241 tcaagaaatg aaagatacaa cctttgaatt acaagaaaat tttagaaggt taaattatcc
     6301 tattaaacaa gttgctaaag acttgcaact taatatcagt gaagttgaat ctcttttaag
     6361 tcttgatgtt acttatcctg gagatgtttg tatgctgaga gattatctag aagatatgtt
     6421 gaaaaaagag ggtaaagaag tttatccatt ttctagatta gcatctcatt cagcaaatcg
     6481 ttggtaccct tatgagacac catggcgtta ttaaagacta aagattaatc catcatcttt
     6541 agagaaataa acaaaaatag tcaatagatt ctttttagaa aaaagctata aaataagtgc
//
LOCUS       DATRUK010000078         6556 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.078, whole genome shotgun sequence.
ACCESSION   DATRUK010000078 DATRUK010000000
VERSION     DATRUK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.078"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            50..1155
                     /locus_tag="VBE34_001765"
     CDS             join(50..326,326..1155)
                     /locus_tag="VBE34_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag2 family transposase"
                     /protein_id="HEO7264458.1"
                     /translation="MSRKVRRHFTDDFKQQIVDLYNVGRKRSSLIKVYELTPSTFDKW
                     VRQAKTTGSFKSVDNLTDEQRELIELRKHNKELEMQLDILKQAAVIMAPKREIITANK
                     DKYSISKMCRWLNMPHSSYYYQAVESVSETEFEETIKRIFLDSESRYGSRKIKICLNN
                     EGITLSRRRIRRIMKRLNLVSVYQKATFKPHSRGKNEAPIPNHLDRQFKQERPLQALV
                     TDLTYVRVGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMFH
                     SDRGKEFDNQLIDEILEAFGITRSLSQAGCPYDNAVAESTYRAFKIEFVYQETFQLLE
                     ELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA"
     gene            1379..2511
                     /locus_tag="VBE34_001766"
                     /pseudo
     CDS             1379..2511
                     /locus_tag="VBE34_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2551..2790
                     /locus_tag="VBE34_001767"
                     /pseudo
     CDS             2551..2790
                     /locus_tag="VBE34_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2989..5457)
                     /locus_tag="VBE34_001768"
     CDS             complement(2989..5457)
                     /locus_tag="VBE34_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO7264459.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGLATKDNQIAYIDD
                     SKGKVKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYHFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTDDNDSGSDHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELVAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YIMPKDGKDYFYARYQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRNQIATIKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFAAPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPEEKQQAD
                     KSNENQQPSEASKEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQPE
                     GVQFYNKNGELVTYDIKTLQQINP"
     gene            complement(5470..6390)
                     /gene="lmb"
                     /locus_tag="VBE34_001769"
     CDS             complement(5470..6390)
                     /gene="lmb"
                     /locus_tag="VBE34_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEO7264460.1"
                     /translation="MKKVFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVNVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGHLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
BASE COUNT         1978 a         1306 c         1207 g         2065 t
ORIGIN      
        1 gacagaaatt ataaagtgtt ctacacttaa gaaaaactgg agaaaaacca tgtctagaaa
       61 agtacgtcgt cacttcaccg atgattttaa gcaacaaatt gttgaccttt acaatgttgg
      121 tagaaagcgt agcagtctca tcaaggtata tgagctaacc ccttccacct tcgataagtg
      181 ggttagacaa gccaaaacaa ctggttcatt caagtctgtt gataatctga cagatgaaca
      241 gcgggagcta attgaactca gaaaacacaa taaagaactc gaaatgcaat tagatatcct
      301 aaagcaagca gcggtgatta tggcacaaaa agggaaataa tcactgctaa taaggataaa
      361 tacagcattt caaagatgtg tcgctggctg aatatgccac actcaagtta ttactatcaa
      421 gccgtggagt cagtatctga aacggagttt gaagaaacta ttaaaagaat tttcctcgat
      481 agcgagtcca gatacggatc cagaaaaatc aaaatatgct tgaataacga aggtatcaca
      541 ctttcacgtc gtcggattcg acgcattatg aagcgactca atttggtttc tgtttatcag
      601 aaagccacct tcaaaccaca ttctagaggc aagaatgaag cccctattcc caaccactta
      661 gacaggcaat ttaagcaaga aagaccacta caggccttag tcactgactt aacctatgtt
      721 cgtgtaggca atcgttgggc ttatgtttgc ctcatcattg acctatacaa ccgtgaaatc
      781 atcggcctgt ctcttggttg gcacaagacc gctgaactcg ttaagcaagc catacaaagc
      841 atcccttacg ccctgaccaa agtcaagatg ttccattcag atcgtggcaa agagtttgat
      901 aatcagttaa ttgatgaaat attagaagcc tttggaatca cacgttcgct tagtcaggct
      961 ggttgtcctt atgacaatgc cgtagctgaa agtacgtatc gtgctttcaa aattgaattt
     1021 gtttatcaag aaacctttca attgctggaa gaactagctc ttaagactaa agactatgtt
     1081 cattggtgga attaccaccg cattcatggt agtctcaact accaaacacc aatgactaaa
     1141 agattaattg cctagaaaaa cactttataa atgttgtaca gaaaagtgtt gccttttcac
     1201 ttaataaaag aaagattgaa ggaacacttc tctttcattc cgaccaaggg tcacaattta
     1261 agtccgggga atttagaaaa ataattgatg acaacaatag gcactgaatc cctaaaatga
     1321 gacaaaagaa aaacactcct gttgaaattt agtaaactag aaacaggagt gttttattat
     1381 ggttaaaaaa gcatattcat gggaaactaa gctggcttgc atcgatatga agaaagcagg
     1441 caagtctaac agagttatta tggagacttt aggcatcaaa aataatagtc aaatctatac
     1501 ttggatgaaa tggtacgaaa acgaggagct ttatcgtttt catcaaggtg ttggcaagca
     1561 atatacctac ggtaaaggct tggaacatct gtctgaagtg gaacaattac aattacaggt
     1621 tgacctctta aaaaagtatc gaggcttgat aaggaaatcg ataaagtaag tcttatcaag
     1681 cttgtggaag actataaaaa caactacccg atagttttca ttctagactg ttttaaggtc
     1741 aagcggtcga cctattaccg ttggaaaaag gagtatgaga agccgcaaaa aaagatgacc
     1801 ttattgaatt aattgaacag ctctgtatgg agaatcactt tatttatggc tatcgtacca
     1861 ttacacgatt gctcaaaaaa atccacggat tgactgttaa tacaaagaaa gtctatcgta
     1921 tcatgaaaaa taatggctgg ctttgtcgga cacgtaccaa gaaagttccc aatttgggta
     1981 aagcttatta tttaacagat aataaattga gtcgggattt ccatgccgat aagcctaagg
     2041 aaaagctcgt aacagatatt acctacctat atttcggtaa ctgtaaattg tatctctcat
     2101 caattatgga tctctataac cgagaaatta tagcttacac tatctcagat tgtcaggaca
     2161 cggattttgt actagacacc cttaatcagt taaaattgcc taaataaagg agcaatctta
     2221 cacagtgacc aaggctcagt gtatacttct atggcttact accaggcttg cacggaaaaa
     2281 gacattatcc gctcaatgtc tcgaaaagga acacctgcag ataatgcctg tattgaatgg
     2341 tttcacaccg tcctaaagac tgaaaccttt tatttccata ataggagaaa atacaacaaa
     2401 gatagtataa caaatattgt taaaaattac attacatttt ataatgaaac tagaattcaa
     2461 cagagattaa acgaccagtc gcctgtacag tacagaaaac tgattgccta aaagtgtttt
     2521 tcttgcgtct cactattagg gtgcagtgcc aatatcatgc attctttttc taatcctgga
     2581 tatccttatg ataatgccgt aactgaagca tttttcaagt atttaaagca tagacaaatc
     2641 aaccgaaaac attatcaaaa tatcaaacag gttcaattag actgctttga atacattgag
     2701 aatttttata acaattacaa cccacatacg gctaatctag gactaacccc taatcagaaa
     2761 gaagaaaatt attttaactc aataaaataa cacagttttc tgtctactta tttgacatta
     2821 gtccaagaat cctcctcttt ttttactatt ctaacatgga taccatttct ttgcaaaaga
     2881 aaaccgctat tatccaactt ttatcactac tcgttacata gaatgacttc aaaatcaccg
     2941 taacttgctt tgacaaagca gtgctttaac aatgagatct tcttttggtt aagggtttat
     3001 ttgttgaagt gtcttgatat cataagttac caattcacca tttttattat aaaattggac
     3061 accttctggt tggaaaatga gatacttagg atcgatatta gctttttgtg ctaattgatt
     3121 gatatgatct tctagggttg ctctatctag accatagtct ggtaaactgt ctataaagtc
     3181 atctgattct ttttcttctt tactggcttc acttggctgt tggttttcat tgctcttatc
     3241 tgcctgttgc ttttcttcag gtttatccgt atcagtagca tcaccagcat ttttctttgc
     3301 caataactct tgagcttgtt gccactcagc ttgggataga tcggatttat taatggttct
     3361 caatgaactg ccatctgctc ttgggatgct aaaggagcca tctttccata caaaagtttc
     3421 ttttgccaaa acatctcgtg gatcgaaaat atagccgtct ggtgctgcaa aacgaccttc
     3481 tgctagggct ttttgaactt cttcagcaga atggataatt tgccagtttg gcataccagc
     3541 acgtttatca agaggcgtaa catttggaat gaccgaacct gactcttcat gccctggctt
     3601 agaccataca tccggacgaa cttcggggtg ttgcatcaca tacttgattg ttgcaatctg
     3661 attgcgcgtc aaccatgaat acggaacgac atggatatga tcaatatgtg ggataacaaa
     3721 cgaacttcca gtatcgtaag tagcattacc agcatgcatc ggcagacttg acacatctac
     3781 agcaagtcgt ggctcaatgc ctgtatcaac aatgtcataa cggtaatgct tcttatcttt
     3841 aagcattagt tcttgttcgg caaaggcaat ctgagtcaaa tcaagttgat aacgagcata
     3901 gaaatagtcc ttgccatctt ttggcataat atagcccact ttaccatctt ttgttacttt
     3961 gcgactcact tttttagtgt caaagagtgg tttttctttg ccttgttcct gatccaaagc
     4021 agcaacaagc tcatcagctt gaccttttgc tttcacccag ttagcgacct catccaactc
     4081 atattgttca agttctccaa atcctatata gtggaaatga tctccgtgtt tagctgtaac
     4141 tcctgattta tccactgaat gaatggattc tttactaaaa acataagcat cactcgtatc
     4201 atatggttta ccatctaagc cttttccgta agctttgatg cgatgaccaa gaaaggtatg
     4261 tgtcacttct ttatctgatg gttttgagtg atctgaacct gagtcgttgt catcagtttg
     4321 gccggctaag tatcgatctg ctaattccat ttcaagaggt gataactgac ttcttgggat
     4381 aatatgataa tgatctccat gaggcaccac atacccaaaa gcgtttgatt tgatcacttg
     4441 agtcggttca aaaatcaacc catcttcttc cacatgacgg tatttcaaat caagacggtg
     4501 tagttgatct aaaagttcct taaaggtttt tcctttaaac tcatctcttt ggtgtttgtt
     4561 ttgtgacgca tcatttggcc taggaggcgc tggatgataa ccaccgttat ctggctgatg
     4621 accttgtcca gggttaggcg tcacatcagg aattggggct ttcctacgac ctggggctgg
     4681 tgtcgggcgg taatcagacg gtctagcacc tcgaccttgt ttttgactcc agtaggcttg
     4741 tgcagcagct agctcacttg gagacaaatc ttttttagga atataatgat agtgattacc
     4801 atgaggtact aaataagcat ctcctaaatc atcaatgata tctgtcggac taaaaatata
     4861 gccatcgtct gtagtatagc gtccttgtct ttttgcttca ttgactgccg caacttcttc
     4921 tttactgaga tgggccactt gagctaaacc tttttcttta gcttctttag ttcctttggc
     4981 tacttgctca gcaatttgtt gtttggttcg aatgtttttg cgcttactac ctggcttgag
     5041 gtaaacataa tagttgccat tgactttaat aacgtaaccg tctaagattt cattgataac
     5101 gtctgattgt ttaaaatggt aattaggatc cgtcatcaac aactcttcac taataatcgc
     5161 atcataagga actttcccat tgtaaaaatg ataatggtca ccgtgtgagg taacataacc
     5221 ttggtcagta attttgacta cgatctgttc agcagagatg ccttcttcag cactgatttg
     5281 atccatcgtt ttgtttgttt taggggcttt taccttacct ttgctatcat caatataggc
     5341 aatctgattg tcctttgttg ctagacccat atgatgctta ccaagctggt aacttccaat
     5401 atgagtagct agtaaaatag cagcaactga gccgatataa ccatatgttt tcttcactaa
     5461 taatctcctt tacttcaact gttgatagag cacttccaaa tttgctctaa gattttctag
     5521 atatgtcttg tttccgcttg gagcagcttc aagtggactt aatgtcttta ctttagctcc
     5581 tgttgatttc gcaatagcat gagcaatttt ggggttgacg ttgtcttctg caaaaatagt
     5641 cttgacgttg tattctttaa caaagtcttg aatttctttc aattggcgag gagagggctc
     5701 ttgctctgga gaaatacccg agataccaag ttgtttcaag ccgaatcgtt tagccagata
     5761 agaaaatgcc gtgtgttgcg tcacaaatgt ttttgagcgc acctttttaa atttttgagt
     5821 gtattcttca gttagttgct ctgcttcttt tttgaaagcc ttagcctttt tagtgtaact
     5881 gtctttgtgt ttaggatcca aatgtcctag ctctttagcg atattaacag cttcctcacc
     5941 agctaaaacg ggatccgtcc aggtatgtgg gtcataaagt gtcgcagggt caatgccttg
     6001 tgtgacttcc atatcttcta gccctttgac tctatctagt gtcagaggtt ttgacgcttc
     6061 aaacacatta acctttgatt tttttaaatt agggtctaga tcccttgccc aagcttctaa
     6121 ggtatgtgaa tggtaaacaa acaaatccgc gtcataaata gctgccacat catttacaga
     6181 cggttcaaag gaatgaatgc ctgcacctga ttggatcatc ctcacatcat ttagatctcc
     6241 agatacttct tttgtcatcg catacattgg gtaaaagctg gttacaactg acatgccttg
     6301 cgtaggctgt ttggggtttg ctgacttatc acaccctgct atcattacta aactcacaac
     6361 catagccatg agaaaaaaaa cttttttcat agtacctcct caattataat ttaaccagtt
     6421 attaaccagt taattttact cctttatcaa tgctttgtca atgacaacta ccatctttta
     6481 tcacatcaac tgaagggata agcaaaaggc aattgttgac agctatttgt cctaacttct
     6541 ttttgatgag gtagca
//
LOCUS       DATRUK010000079         6361 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.079, whole genome shotgun sequence.
ACCESSION   DATRUK010000079 DATRUK010000000
VERSION     DATRUK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.079"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            105..764
                     /locus_tag="VBE34_001770"
     CDS             105..764
                     /locus_tag="VBE34_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HEO7264461.1"
                     /translation="MSLEDYYLLSKKFSQVMTLDSHSSDDGYTIRSLYFDSLEDVDWQ
                     EKEDGVEIRRKIRLRNYGSQSNFAKLEMKQKQGANQKKRSLKLSKTDAQALINGNNSV
                     LLNYPDTFAAECYSRITTQLYQPKTVIEYQRKAFVAKENNIRITFDHHIIGTEASYDI
                     FSEDLNQNYLLDPYLVILEVKYNGFLLSYIKELLDSVDRSELSASKYCLGRAVSKHYR
                     F"
     gene            815..1492
                     /locus_tag="VBE34_001771"
     CDS             815..1492
                     /locus_tag="VBE34_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEO7264462.1"
                     /translation="MKDLLRNSLEQSGNLSFQDMILHILVAALLSVVIYVSYAYTHSG
                     TAYSKKFNVSLMTLTVLTATVMTVIGNNVALSLGMVGALSVVRFRTAIKDSRDTVYIF
                     WTIVVGICCGVGDYVVAALGSSVIFILLWVMGRVKNENRMLLIVKCDRTLEVDLEGIF
                     FQYFDGKAVQRVKNSTTNTIEMIFEISRKDYDKQLHVDNQLTEKVYQLGNIDYFNIVS
                     QSDEING"
     gene            1485..2930
                     /locus_tag="VBE34_001772"
     CDS             1485..2930
                     /locus_tag="VBE34_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEO7264463.1"
                     /translation="MGRLMRGKVIYGTTLIGLFLFLFFYFWIPKHHIERIHHHRIKQV
                     DAKSDLTGFKTHLPIISIDTKQQVIPLVTKEGGKYVKARDNINVDIELRDSPSRSHHL
                     SEKPRIRTKGLISYRGNSSRYFDKKSLKVKFVTNKLKEKKHRLAGMPKESEWVLHDPF
                     LDRTLLRNYLSYNIAGEIMSYAPNVRYCELFVNGEYQGVYLAVENIEQGEQRVPIEKS
                     DKKLHKTPYIVAWDREHKAKQKLDNYVHYTHQSGISALDVKYPGKQRLTSKQLEFINK
                     DINHIEKVLYSYDFSQYPKYIDRESFANYFVINEFFRNVDAGKFSTYLYKDLRDRAKL
                     VVWDFNNAFDNQIEGRVDEADFTLTDAPWFNMLIKDKAFIDLVVHRYKELRKGVLATE
                     YLSNYIDETRHFLDPAIDRNYKKWGYVFDLKNTDPRNYLIPTERNVTSYHKSVEQLKD
                     FIKKRGRWMDRNIETLYQYSAPSKNTNTLLE"
     gene            2937..5096
                     /locus_tag="VBE34_001773"
     CDS             2937..5096
                     /locus_tag="VBE34_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264464.1"
                     /translation="MKKYLSIVALLTILFSSIYYLYYFDGSLYLPKGLLKENTRTNFV
                     VKGDTVLHKPTNKPFVVKGVDVESSLAGYHHNDFPITQKTYREWFHLISNMGANTVRV
                     KVPMNVAFYDALYHHNKESKRPLYLLQGIRIDSYRNNASITAFNDNYRGYLKREAKGV
                     VDILHGRKQVWNTDFGSRHYHYDLSPWVLGYVVGDDWNSGTVAYTNHQEKKTQYKGRY
                     FKTSVAANPFEVMLAQVMDELTHYETAKYGWQHLISFSNSPTTDPFHYRKPFEAQAPK
                     YVQLNVENIQANSNVKAGMFAAYKAIDFHPRYKDYLLFDKENISKEDRQKIKELSLSQ
                     GYVKLLNAYHKIPVLVTGYGYSTARGIAQKEIDKRPLPINEKEQGQRLLEDYESFISS
                     GSFGATINAWQDDWNARAWNTSFATNKHSQFLWGDAQVFNQGYGLLGFKNAKHHYQVD
                     GKRGKGEWKHPLMTSATGDDLYASSDESYLYLAIKTKPEKLKEKRLLPIDITPKSGSR
                     KMNGSKVTFSKSSDFVLSIDPNGKSELFVQERYNALKANYLRQLNGKDFYAFPPKKNS
                     SNFEQINMVLRNTKIVEDMEKVKATERFLPTHPTGLLKTGTTDRHQKTFDSQTDISFG
                     KDFIEVRIPWQLLNFSDPSSQKIHDDYFKHYGVKELEIESIALGLGANSKENTLIKMA
                     DYRLKNWERPDTKTFLKDSYYSIKKEWSKERERTYGT"
     gene            5236..6361
                     /locus_tag="VBE34_001774"
                     /pseudo
     CDS             5236..6361
                     /locus_tag="VBE34_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643060.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT         2175 a          913 c         1226 g         2047 t
ORIGIN      
        1 atttattttt gcttaattta ttgttaatat ttatatataa attaatattt aattaaacag
       61 aggtgagtga tgaacgaagt tcttagacag gaaaaaaata tttaatatca cttgaagatt
      121 actatctttt atctaagaaa ttttcccaag taatgacgct cgattcgcat agttcagacg
      181 acggctatac tatccgttcg ctatactttg attctttaga agatgttgat tggcaagaaa
      241 aggaagatgg tgttgagata cgaagaaaga ttcgtttgcg caattatggt agtcaaagca
      301 attttgcgaa attagaaatg aaacaaaaac aaggtgctaa tcaaaaaaag cgctctctaa
      361 aattaagcaa gacagatgct caagcgctga ttaatggtaa caatagtgtt ttattaaatt
      421 atccggatac atttgctgcg gagtgttatt caaggataac aacgcaactt tatcaaccta
      481 aaacggttat tgagtaccaa agaaaagctt ttgttgctaa ggaaaacaat atccgaatca
      541 cctttgacca ccatataata ggtacagagg ctagttatga tattttctca gaggatttaa
      601 atcaaaatta tttattagat ccttatcttg ttattttaga agttaaatat aatggatttt
      661 tattgtctta tattaaagaa ctcttagatt cggtagatag aagtgaactt tcagcgagta
      721 agtattgttt aggaagagct gttagtaaac attatcgttt ttagtgaaaa agtatatacg
      781 ttaattatta ttaaatgtga gaggataaca aaaaatgaaa gacctattac gaaatagtct
      841 agagcaaagt ggaaatttaa gttttcaaga tatgatttta catattcttg tagcagcttt
      901 attgagtgta gttatttatg tttcctatgc ttatacgcat agtggaactg cctatagtaa
      961 aaagtttaat gtttcattaa tgacattgac ggtcttgact gcaacagtaa tgaccgttat
     1021 tggtaataat gtagccttgt cattgggtat ggtcggtgcc ttgtcagttg ttcgttttag
     1081 gacagccata aaagattcaa gagatacagt ttatattttt tggaccatag ttgttggtat
     1141 ctgttgtggt gtcggtgact atgtggtagc tgcattagga agtagcgtta tctttatctt
     1201 attatgggtt atgggacgtg ttaaaaacga gaatcgtatg ttattgattg tgaagtgcga
     1261 tagaacacta gaagttgatt tagaaggaat tttcttccaa tattttgacg gaaaagctgt
     1321 tcagcgtgtt aaaaattcaa caactaatac tattgaaatg attttcgaaa tctctagaaa
     1381 agattacgat aagcaactcc atgtagataa tcagttaact gaaaaagtgt accaattggg
     1441 aaatattgat tatttcaaca ttgttagcca aagcgacgaa atcaatgggt agacttatga
     1501 gagggaaggt tatttacggc acaaccctta taggtctttt tctattctta tttttctatt
     1561 tttggattcc taagcatcac atcgagagaa tacatcatca tcgtataaag caggtagatg
     1621 cgaagagtga tttaacagga tttaaaaccc atttgcccat tatcagcatt gatacaaagc
     1681 aacaagttat tcctcttgtt acaaaagaag gcggaaaata tgtcaaagct agggataata
     1741 ttaatgttga tatcgaatta cgggattctc caagtagatc acatcattta tcagaaaagc
     1801 cgagaattag gacaaaaggg ttaatatcat atagaggaaa ttcctctcgt tactttgata
     1861 agaagtcatt gaaagttaag tttgttacta ataagttaaa ggaaaagaag catcgattag
     1921 caggaatgcc taaagaatcg gagtgggtat tgcatgatcc ctttctagac agaacattat
     1981 taagaaatta tctgagttat aatattgctg gtgagattat gtcctatgcc ccaaacgttc
     2041 gctactgtga gttatttgtc aatggtgagt atcagggagt atatctagca gttgagaata
     2101 tcgaacaagg tgagcaacga gttcccattg aaaaaagtga taaaaagttg cataaaacac
     2161 cttatatcgt tgcttgggat cgtgaacata aggcaaagca aaagttagat aactatgtac
     2221 attatacaca ccagtctggt atctcagctc ttgatgtgaa atatccaggt aaacaaagat
     2281 tgacatcaaa acaattagag tttattaata aggacatcaa tcatattgaa aaagtccttt
     2341 attcttatga tttttcacaa tatcctaaat atattgatag agaatcattt gcaaattatt
     2401 ttgtcatcaa tgaatttttt agaaatgtgg atgcgggtaa attttcaacc tatctatata
     2461 aagatcttcg tgatagagca aaactagtag tttgggattt taataatgct tttgataatc
     2521 aaattgaagg acgtgttgat gaggcagatt ttaccttgac ggatgcaccg tggttcaata
     2581 tgctcattaa ggacaaagca tttatagacc tggttgttca tcgctataaa gaattacgaa
     2641 agggagttct agcgacagaa tatttatcta attacataga cgagacaagg cattttttag
     2701 atccagctat tgatcgaaat tataaaaaat ggggctatgt ctttgattta aagaatactg
     2761 atccaaggaa ttatttgatt cctactgaac gcaatgtgac aagttatcac aaatcagtag
     2821 aacaattaaa agattttatc aaaaagcgtg gcagatggat ggatagaaat attgagacct
     2881 tgtatcaata tagtgcacca tcgaaaaata ctaatacttt actggagtag aacattatga
     2941 aaaaatatct aagtattgta gcccttttaa ctattttgtt tagctctatt tattatttgt
     3001 attactttga tggtagtttg tatttaccaa agggcttatt aaaagaaaat acaagaacta
     3061 actttgttgt taaaggtgat actgtacttc acaagcccac caataaacct tttgttgtta
     3121 aaggagtaga cgttgagtct tccttagcgg gttatcatca caacgatttt cctattactc
     3181 aaaaaacgta tcgtgaatgg ttccatttaa tttccaacat gggggcaaat actgtaagag
     3241 tcaaggtacc gatgaatgtt gcattttacg atgccttata tcaccacaac aaagaatcaa
     3301 agaggccact gtatttgttg caaggaatac gtatagattc ttatcgcaat aatgcttcta
     3361 taacagcttt taatgataat tatagggggt atttaaaacg agaagcaaaa ggcgttgtgg
     3421 atattctcca tgggcgtaag caagtatgga atactgattt tggtagccgt cattatcatt
     3481 atgatcttag tccttgggta cttggttatg tcgtagggga tgattggaat agtggtactg
     3541 tcgcttatac taatcatcaa gagaaaaaaa cgcaatataa aggacgttat tttaaaactt
     3601 ctgtggcagc taatccattt gaggtcatgc tagctcaagt aatggatgaa ttgacacatt
     3661 atgagacagc taaatatggt tggcaacatt tgattagttt ttcaaactca ccaacaacag
     3721 acccttttca ttatcgaaaa ccatttgagg cacaggctcc taaatacgta caactaaatg
     3781 tagaaaatat tcaagctaat tcaaatgtta aagcaggtat gtttgcagca tataaagcta
     3841 ttgatttcca tcctcgatac aaggattatc tattatttga taaagagaat atcagtaaag
     3901 aagatagaca aaagattaaa gaactttctt tgtcacaggg atacgttaaa ctgctaaatg
     3961 cttatcacaa aatccctgtt ctagtcacgg gttatggcta ttcgacagcg agaggtattg
     4021 cccaaaaaga aattgataaa cgtcctctgc cgattaatga aaaagaacaa ggtcagcgtt
     4081 tactagaaga ttatgaatct tttatatcat ccggtagttt tggagcgact atcaatgcat
     4141 ggcaagacga ttggaatgca agggcgtgga atacatcttt cgccacaaat aaacatagtc
     4201 aattcctatg gggggatgca caagtattta atcaaggtta tggtttatta ggctttaaaa
     4261 acgcaaaaca tcattatcaa gttgatggta aaagaggcaa aggagagtgg aaacatcctc
     4321 tgatgactag tgcaacagga gatgacttat atgctagcag tgatgaaagc tatctctacc
     4381 ttgcgattaa aacaaaacct gaaaaactaa aagaaaaacg attattacca atagatatta
     4441 caccaaaatc tggtagtaga aaaatgaatg gtagtaaggt cacattttct aaatctagtg
     4501 actttgtatt gtctattgat ccaaatggca agtctgaatt atttgtccaa gagcgctata
     4561 atgccttaaa agcgaactat cttcgacagc ttaacggtaa agatttttat gctttcccac
     4621 caaagaagaa cagtagtaat tttgagcaga taaatatggt attgagaaat acaaagattg
     4681 ttgaagacat ggaaaaagta aaagcaacag agaggttctt accaactcat cctactggtc
     4741 ttctcaaaac aggaacaact gataggcacc aaaaaacatt tgattcacaa acagatattt
     4801 cgtttggaaa ggactttata gaggtcagaa ttccgtggca gttgttgaat ttttctgatc
     4861 catcatctca aaaaattcac gatgattact ttaaacatta tggtgtgaag gagttagaaa
     4921 ttgagagcat tgctttagga ttaggtgcta atagcaaaga aaacacactg ataaagatgg
     4981 cagattatcg tttgaaaaat tgggagagac ccgataccaa aaccttttta aaagactcct
     5041 attatagtat taagaaagaa tggtctaaag aaagagagag aacatatggt acataatatt
     5101 ttatattttt acattttgat ttgcttagca attattgttt ttaacattca ctatatctta
     5161 agacaacgat tagataagct tagttggctt cgtattcgga aaaaggatat aaatatcaaa
     5221 taaaggggat tttctatggc attatttttt accttcctaa aggtatctca agtcattttc
     5281 ttagtattat tgattgccta tgccagcttt ttagtgattt cattagtcat tggaggtgtt
     5341 catctctatg aaaaaaatga gcgcaaaaaa agtttaacta gaaataaaga aaaaattcca
     5401 atttcaatta ttgtaccagc ctataatgaa tcaacaacca ttgtgagttc gattgattcg
     5461 ttattgcacc ttgattatga agcgtatgaa attatcgttg tggatgacgg ttccagtgac
     5521 aatacttcag atgttctcaa ggaagagttt gcattaatga aaatctcgaa tacgattgat
     5581 tcaattattg ctactcaaac ttgtaaagat gtctttcaga gacaggttgg taaggttaaa
     5641 ctgacactca ttgttaaaga aaatggtggc aaaggggatg ccttaaatat ggggatcaat
     5701 gctgctaatt acgattattt tctatgtcta gatgcagatt ctatgttgca ggtagattct
     5761 ttgagtcaaa ttagcaaatc catacaggta gatccgacgg ttatagcagt gggagggctt
     5821 gtccaagttg cccaaggtgt taagattgag caggggaaag tagcatctta tcgtttgcct
     5881 tggagaatta ttccttgtgc acaagcactt gagtatgatt catcattttt aggagctcga
     5941 atctttttag actatttaag agctaattta attatttcgg gggctttcgg tctttttaag
     6001 aaagaccttg ttaaagcggt gggaggatat gacacccaga ctctaggaga agatatggag
     6061 ttggtgatga aattacattt cttctgtcgt aataataata ttccttatcg tatttgttat
     6121 gaaacagatg ctgtttgttg gagtcaagca ccaacgaatt taggggattt acgtaaacag
     6181 agacgccgat ggtatcttgg cttgtactaa tgtttaaaaa aatataaaag tatttttgcc
     6241 aattatcgct ttggagctgt aggatcaatt tcttatattt actatatttt atttgaatta
     6301 ctgacccctt ttatagaatg ttttggtatt gtcattatat ttttgtcctt attatttaat
     6361 c
//
LOCUS       DATRUK010000080         6212 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.080, whole genome shotgun sequence.
ACCESSION   DATRUK010000080 DATRUK010000000
VERSION     DATRUK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.080"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            103..1245
                     /locus_tag="VBE34_001775"
     CDS             103..1245
                     /locus_tag="VBE34_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEO7264465.1"
                     /translation="MKVVVAIDSLKGSLSSLEAGNAIKESINEVIPGADVEVHPLADG
                     GEGTVEALTLGMGGTIEIIPVKGPLGEKVHASYGIIPQRQLAIIEMAAAAGITLIATE
                     ERNPLHTTTYGVGEMINDAISKGCRHFIIGIGGSATNDGGAGMLQALGYALLDKDNQE
                     ISLGAQGLADLKSISTDKVIEELKECDFKIACDVTNPLCGAQGCSSIFGPQKGADEDM
                     ITKMDTWLSNYATLATSVSEKADATIEGTGAAGGLGFAFLAFTNATLEPGIDIILSEI
                     NIEKAISEADLVVTGEGRLDGQTVMGKAPIGVAKLAKKYGKKVVAFSGSVTEDAILCN
                     QHGIDAFFPIVRRLISLDEAMSKEVAYKNMKETATQVFRLINLYNH"
     gene            1270..2526
                     /locus_tag="VBE34_001776"
     CDS             1270..2526
                     /locus_tag="VBE34_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEO7264466.1"
                     /translation="MEATFTTTGALIGLALAILLIIKKVHPAYSLILGALVGGLIGGG
                     DLVTIVNTMVLGAQGMMSSILRILTSGILAGALIKTGSAEKIAESIIKKLGQQRAITA
                     LAIATMIICAVGVFIDIAVITVAPIALAIGKKANLSKSSILLAMIGGGKAGNIISPNP
                     NTIAASEAFKVDLTSLMVQNIIPAIAALVVTIILAKIVSKKNNDISYDSEEQVGSDLP
                     AFLPAISGPLVVICLLALRPLFGITIDPLIALPLGGLISILATGYLKETVPFVEYGLS
                     KVVGVSILLIGTGTLSGIIKASNLQFDMIHLLEFLNMPTFILAPLSGIFMGAATASTT
                     SGTTIASQTFAETLIKSGVPAVSGAAMIHAGATVLDSLPHGSFFHATGGAVNMAIKER
                     MKLISYEALIGLTSTIVAVVYYCFFG"
     gene            2627..3691
                     /locus_tag="VBE34_001777"
     CDS             2627..3691
                     /locus_tag="VBE34_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO7264467.1"
                     /translation="MKQTKKEFILGLTIIITLTLLIMLIFLVMFNKSSNSKLKQTKDR
                     RILSTSTTRTSSSSHDQKIVEKSLEKKKMVMEQDMELMNAYRLYYDYAHLSLKEVVLA
                     YMSEYGIEKNSVAFSYKNLKTGQRDAMNDTQAMTAGSTYKLPLNMLVVDAVVKGKLSM
                     EERFDITETDYEYIGEHNNYVAAFSGAMSIDDMQKYSLVYSENTPAYALAERIGGMDS
                     AYSKFGRYGQSKGDIKNIQKNGNKVTTDYYIQVLDYLWKHRKKYNSLITYLEEAFPTD
                     YYRALIPSDVVVAQKPGYVREALNVGAIVKEEVPYIVAIYTAGLGGSTQEDSEINGVG
                     LYQLEQLCFVINQWHRVNMN"
     gene            complement(3746..4081)
                     /locus_tag="VBE34_001778"
     CDS             complement(3746..4081)
                     /locus_tag="VBE34_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7264468.1"
                     /translation="MTYLQYVILLVLWEEDHRHMKELSYALSLNSNTLTPVLKRMENR
                     GWIVREKPRDDKRQLIIHLSNKGIESQDAVVNAVQSCGYFFSEISPESYQKTVSALSS
                     LNQVIKSHL"
     gene            complement(4270..5298)
                     /gene="queA"
                     /locus_tag="VBE34_001779"
     CDS             complement(4270..5298)
                     /gene="queA"
                     /locus_tag="VBE34_001779"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEO7264469.1"
                     /translation="MNTNDFDFYLPEELIAQTPLEKRDASKLLVIDHKNKTMTDSHFD
                     HILDELKPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTEGDQWEVLAKPAKRL
                     RVGNRVSFGDGRLIATVTKELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     RDRYQTVYAKENGSAAAPTAGLHFTKELLEKIETKGVKLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSKEAADTLNAVKESGGRIVAVGTTSIRTLETIGSKFNGELKADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGHDFVLEAYNHAVEERYRFFS
                     FGDAMFVK"
     gene            5451..6131
                     /locus_tag="VBE34_001780"
     CDS             5451..6131
                     /locus_tag="VBE34_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEO7264470.1"
                     /translation="MKEAKNFSQQLNILRAGVLGANDGIISVAGVVIGVASATHNLWI
                     IFLSAASAILAGAFSMAGGEYVSVSTQKDTEQAAVAREEKLLENNPELAKKSLVDIYL
                     AKGESHEHAQWLVDKAFSKNAIEHLVEEKYGIEFGEYTSPWHAAISSFIAFAIGSIFP
                     TITILLLPFSVRIVGTVIIVIVSLLSTGYVSAKLGQAPTVPAMRRNVMIGCLTMLATY
                     VIGQLFSI"
BASE COUNT         1953 a          994 c         1241 g         2024 t
ORIGIN      
        1 agtctatatt ttttgttatt tgattttgtt actataattg agtgatataa aatagtgaaa
       61 aaataacaaa gattcacaaa atattttcaa ggaggttttc aaatgaaggt agtagtagct
      121 attgattctt tgaaagggag tctttcttct ttagaagctg gcaatgctat aaaggagtca
      181 attaatgaag taatcccagg ggcggatgtg gaagtacatc cattagcaga cgggggagaa
      241 ggaactgttg aagctttgac gctaggtatg ggggggacga tcgaaataat ccctgttaag
      301 gggcctcttg gagaaaaagt acatgcgagt tatggtatta ttccacagcg tcagttagct
      361 attattgaaa tggctgcagc tgctggtatt accctaattg ctactgaaga gagaaatcct
      421 ctccacacaa ccacttatgg agttggagaa atgataaatg atgctataag taaaggctgt
      481 aggcacttta ttattggtat tggaggtagt gctactaatg atggtggggc aggtatgcta
      541 caagctttgg gatatgcatt attagataaa gataatcaag aaatcagttt gggagcacaa
      601 ggattggcag atttaaaatc tatctctact gataaggtta ttgaggaatt aaaagaatgc
      661 gattttaaaa ttgcatgtga tgtaacaaat cctttatgtg gtgctcaagg ttgtagttcg
      721 atttttggac cgcaaaaagg tgctgatgaa gatatgatta caaaaatgga tacttggtta
      781 agtaattatg ctactttagc tacttcagtt agtgaaaaag cagatgcaac tattgaagga
      841 acaggtgcag cgggtgggct tggatttgcc tttttagcct ttacaaatgc caccttagaa
      901 cctggtattg atattattct atcagaaatt aacatagaaa aagcgatttc tgaagctgat
      961 ttagtagtta ctggtgaagg tcgtttggat gggcaaacgg tgatgggaaa agctccaatt
     1021 ggcgtagcaa aattagctaa aaaatatggt aagaaagtag tggcattctc tggatctgtc
     1081 accgaagatg ctatactttg taatcaacat ggtattgatg ctttttttcc aatcgtacga
     1141 cgccttatca gcttagatga ggctatgtct aaagaggtag cttataaaaa tatgaaagaa
     1201 actgctactc aagtctttag attaatcaat ttgtataatc attaatattg aaaggaattc
     1261 tgataaatta tggaagctac atttacaaca acaggggctt taataggatt agcgcttgct
     1321 attttactga ttatcaaaaa agttcatcct gcctatagtc tcattcttgg agcattagtt
     1381 ggtggtttga ttggtggcgg tgatttagtt acaattgtta atactatggt attaggtgcg
     1441 caagggatga tgtcatccat attacgcatt ttaacgtcag ggattttagc gggtgcttta
     1501 attaaaaccg gatcagctga aaaaattgca gaaagtatca ttaaaaaatt aggacaacag
     1561 cgagccatca cagctttagc catcgcaaca atgattattt gtgcagtagg tgtctttatt
     1621 gatattgcag ttattacagt tgctccgatt gcattagcaa taggtaaaaa agctaatctt
     1681 tctaaatcta gcatactgtt agcaatgatt ggtggtggta aggctgggaa tattatttca
     1741 cccaatccta atacaattgc tgcttcagaa gcatttaagg ttgatttaac aagcttaatg
     1801 gtacaaaata ttattcctgc aattgcagct ttagtagtta cgattatatt agcaaagatt
     1861 gtttccaaga aaaataacga tatttcttat gatagtgagg agcaagtggg ctctgatctg
     1921 ccagcattct taccggctat ttctggtcca cttgtggtta tctgcttatt agctcttcgt
     1981 cctttatttg gaatcactat tgatcccttg attgcactgc cgttgggtgg tctaattagt
     2041 attttagcaa caggttacct aaaagaaacg gtaccgtttg ttgaatacgg tttatcaaaa
     2101 gttgttggcg tttcaattct tttgattgga acaggtacac tttcaggtat tattaaagca
     2161 tctaatctac aatttgatat gattcatctc cttgaatttt taaatatgcc gacctttatc
     2221 cttgccccac tctcaggtat ttttatggga gcagcaacag catcaacgac ttctggaaca
     2281 actattgctt cacaaacgtt tgctgaaacc ttgattaaat caggggtacc ggcggtttcg
     2341 ggtgcagcta tgattcacgc aggtgcgact gttctggatt cgctacctca tggatccttc
     2401 ttccatgcga caggcggagc tgtcaatatg gctataaaag aacgtatgaa actaattagc
     2461 tatgaagctt tgattggatt aactagcaca atagttgctg tcgtttacta ttgtttcttt
     2521 gggtaaaatt tggatgaata cgtgatgttt attatcactg tttgaataga attctcattg
     2581 attttttcta attttcttac tataataaat tagtaggaga ggtgttgtga aacaaacaaa
     2641 gaaagaattt attttaggat taaccattat tattaccctt actttattaa tcatgctgat
     2701 ttttttagta atgtttaata agtccagtaa ctctaagttg aagcagacaa aagatagaag
     2761 aatattatct acttcaacca caaggacatc cagtagctca catgatcaaa aaattgtaga
     2821 aaagtctctt gaaaaaaaga aaatggtcat ggaacaagat atggaattga tgaatgccta
     2881 taggctctat tatgattatg cccatttgtc cttgaaagag gttgttctgg cctatatgtc
     2941 tgaatatggc attgaaaaaa atagtgttgc tttttcttat aaaaatttaa aaacaggaca
     3001 aagggacgct atgaatgata ctcaggctat gacagcaggg agcacctata agttacctct
     3061 taacatgttg gtagtagatg cagtggtcaa gggcaagttg tccatggaag aacgctttga
     3121 tattaccgag actgactatg aatatattgg tgaacataat aattatgttg ctgctttctc
     3181 gggggcaatg tctattgatg atatgcaaaa atactcttta gtttattcag aaaatacacc
     3241 cgcctatgct ttagctgagc gtattggtgg catggatagt gcttattcaa agtttggtcg
     3301 ttatggccaa tctaaaggag acatcaaaaa tatacaaaaa aacggtaata aagtgacaac
     3361 agattactat attcaagtgc tagactatct ctggaaacat cgtaaaaagt ataatagttt
     3421 aattacttat ttagaagaag cttttccaac ggattattat cgtgctttga taccatctga
     3481 tgttgttgtt gctcaaaagc cagggtatgt ccgtgaggcg ttgaatgttg gagcaattgt
     3541 caaagaagaa gtgccttata tcgttgctat ttatactgca ggtttaggtg gctctactca
     3601 ggaagattca gaaattaatg gagtgggact atatcagcta gaacaattat gttttgttat
     3661 taatcaatgg catcgtgtta atatgaatta gaaataaatc tatcaaaata ataaaaacct
     3721 cttagattaa ctatctaaga ggtttttata aatgtgattt tataacttga ttaagtgaag
     3781 aaagggcaga aacagttttt tgataagatt caggactaat ttctgagaaa aaatacccgc
     3841 acgattgcac cgcattgaca acggcatctt gtgactcaat acctttgtta cttagatgaa
     3901 taatgagttg ccttttgtca tctcttggtt tttctcgtac aatccaccca cgattttcca
     3961 tacgctttaa aactggagtt agggtattcg aattaagaga tagtgcataa ctaagttcct
     4021 tcatatgccg atgatcctct tcccaaagga ctaacaagat aacgtattgg agataagtca
     4081 attgaaattc atccaaagct ttttggtaaa atttattgaa aagtcgatgt gtttcactaa
     4141 ccgtaaaaca taatcgctcg tctaaaatac ttcgattact cattatcata ttgtagcatt
     4201 ttggaggcgt tatagtatgt caatcgtgtt aaaaaacgat aaaacttttt cctaattgaa
     4261 agcctattct tacttaacaa acatagcatc accaaaactg aagaaacgat atcgttcttc
     4321 aactgcatgg ttgtaagctt ctagtacaaa gtcatgtcca gcaaatgctg atactaacat
     4381 gacaagagta gactttggta agtgaaagtt ggttgaaaaa gcgtctacaa ccttgaactg
     4441 ataaccaggc ttgataaaaa tatttgtcca ccctgagtcg gcttttaatt caccattgaa
     4501 tttagaacca attgtctcta atgtgcgaat agaagttgta ccaacagcta caatacgtcc
     4561 accagattct ttaactgcat tgagagtatc agcagcttct ttagataatt ggtaaaactc
     4621 agaatgcatt tcgtgttcat caagattatc aactgaaaca ggacggaaag ttccaagtcc
     4681 aacgtgaagc gtcaaataga caagcttcac accttttgtt tcaatttttt ccaatagttc
     4741 ttttgtaaaa tgaagaccgg ctgttggtgc agcagcagaa ccattttctt ttgcatacac
     4801 agtctgatag cggtcacgat cttctagttt ttcatgaata tatggcggaa gtggcatttc
     4861 gcccaaactt tcaaggactt ctagaaaaat accatcatat gagaattcta caatacgccc
     4921 accatgttct aattcctttg tgactgttgc tattaaccga ccatcaccaa atgaaactct
     4981 gttccctacg cgtaagcgtt tagcgggttt agcaagcact tcccattgat cgccctctgt
     5041 atttttgaga agtaataact ctacatggcc gtgtgtatct ggcttttcac cataaaggcg
     5101 agcgggcaga acacgtgtgt tattcattac taaagcgtca ccaggcttga gttcatctaa
     5161 gatatgatcg aaatggctat ctgtcatcgt cttattctta tgatcaatga ctaatagttt
     5221 agaagcgtct cttttttcaa gtggtgtttg ggcaataagt tcttctggta aataaaaatc
     5281 aaaatcgttt gtattcatga tttctcctat ctataagtaa gcttttctat tatatcacgt
     5341 tcggtaaaaa tgacccaact aatcttctta tttagaatga ttataaaaaa taattgtaat
     5401 cattctaaat aagtggtata ataattgtca gaaagtgaga agtagagaag atgaaagaag
     5461 caaaaaattt ttcccaacaa ttgaatattt taagagctgg tgttcttggt gctaatgatg
     5521 gtatcatctc tgtagctggt gttgttattg gtgttgcgag tgcaactcat aacctttgga
     5581 ttattttttt atcagcagct tccgcgattt tagctggtgc attttctatg gctggcgggg
     5641 agtatgttag tgtttctact caaaaagata ctgaacaagc agcagttgcg cgtgaagaga
     5701 aactactaga aaataatcca gaacttgcca aaaaatcctt agttgatatc tacttagcaa
     5761 aaggagaaag tcacgaacat gctcaatggc tagttgacaa ggctttttca aagaatgcta
     5821 ttgagcattt agttgaagaa aaatatggta ttgagttcgg tgaatataca agcccctggc
     5881 atgctgctat atctagtttt atagcttttg ccatcggatc aatttttcca actataacta
     5941 ttctgttatt accattttcg gtacgaattg taggaacggt aataattgtt attgtttcac
     6001 tattatcaac tggttatgta agtgctaagt taggccaagc ccctacagta cctgccatgc
     6061 gtcgaaatgt gatgattggt tgtttgacaa tgttggcaac ctatgtcatt ggacaattat
     6121 tttcaatata aaatattaat cgtggagtta ttgtctaaac tagaatggac agtaattctt
     6181 tttagtttta taacgaaatt aagaagtttt ta
//
LOCUS       DATRUK010000081         6162 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.081, whole genome shotgun sequence.
ACCESSION   DATRUK010000081 DATRUK010000000
VERSION     DATRUK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.081"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            103..1248
                     /locus_tag="VBE34_001781"
     CDS             103..1248
                     /locus_tag="VBE34_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7264471.1"
                     /translation="MGQEPIIEYQNINKVYGENVAVEDINLKIYPGDFVCFIGTSGSG
                     KTTLMRMVNHMLKPTNGTLLFKGKDISTINPIELRRRIGYVIQNIGLMPHMTIYENIV
                     LVPKLLKWSEEAKRAKARELIKLVELPEEYLDRYPSELSGGQQQRIGVIRALAADQDI
                     ILMDEPFGALDPITREGIQDLVKSLQEEMGKTIILVTHDMDEALKLATKIIVMDNGKM
                     VQEGTPNDLLHHPATSFVEQMIGEERLLHAQADITPVKQIMLNNPVSITTEKTLTEAI
                     TLMRQKRVDSLLVTDNGKLIGFIDLESLSSKYKKDRLVSDILKHTDFYVMEDDLLRNT
                     AERILKRGLKYAPVVDHENNLKGIVTRASLVDMLYDIIWGDTETEDQ"
     gene            1248..1883
                     /locus_tag="VBE34_001782"
     CDS             1248..1883
                     /locus_tag="VBE34_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7264472.1"
                     /translation="MVNFLSQYGMQILVKTWEQVYISFFAIALGIAIAVPLGVVLTRF
                     PKVAKIIIAIASMLQTIPSLALLALMIPLFGIGKVPAIVALFIYSLLPILRNTYIGMN
                     NVNPTLKDCAKGMGMKPIQSIFQVELPLATPIIMAGIRLSTIYVIAWATLASYIGAGG
                     LGDLIFSGLNLFQPKLILGGTIPVIILSLIIDYLLGLLETALTPRTTRREA"
     gene            1886..2812
                     /locus_tag="VBE34_001783"
     CDS             1886..2812
                     /locus_tag="VBE34_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7264473.1"
                     /translation="MLKKSHFLQIFTLCLALLTISGCQLTDTKKPGHTTIKVAAQSST
                     ESSIMANIVTELIHHELGYNTTLISNLGSSTVTHQALLRGDADIAATRYTGTDITGTL
                     GLKAVKDPKEASKIVKTEFQKRYNQTWYPTYGFSDTYAFMVTKEFARQNKITKISDLK
                     KLSTTMKAGVDSSWMNREGDGYTDFAKTYGFEFSHIYPMQIGLVYDAVESNKMQSVLG
                     YSTDGRISSYDLEILRDDKKFFPPYEASMVVNNSIIKKDPKLKKLLHRLDGKINLKTM
                     QNLNYMVDDKLLEPSVVAKQFLEKNHYFRGDK"
     gene            2813..3463
                     /locus_tag="VBE34_001784"
     CDS             2813..3463
                     /locus_tag="VBE34_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7264474.1"
                     /translation="MKQMNTFQQFIYYFQHNGSYILEQFIHHFLISIYGVILAAIVGI
                     PLGIFIARRGKIKTFVMGIANVIQTIPSLAMISIIMLGLGLGIKTVVATVFLYSLLPI
                     ITNTYTGIRNVDSDLLDAAKGMGMTKRQRLFMVELPLSISVIMAGLRNALVVAIGITA
                     IGAFVGGGGLGDIIIRGTNATNGGAIILAGSLPTALMAIFSDLILGGIQRMLEPRK"
     gene            complement(3525..4706)
                     /locus_tag="VBE34_001785"
     CDS             complement(3525..4706)
                     /locus_tag="VBE34_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7264475.1"
                     /translation="MNINGIKLLSSRAVSKLGDVLYDYGNSTWIASIGGLGQKILGIY
                     QIVELLVSIVLNPFGGALADRFQRRKILLITDAICAIMCFLLSFIGDDKVMVYGLIVA
                     NAILAVSNAFSSPAYKSYIPEIVDKADIITYNANLETIVQIISVSSPVLGFLIFNNFG
                     IRITLIVDAITFLISFLFLYAIKVERVQLSKQEKVAIKNILADIADGFTYIKKEKEIM
                     FFLIIAALLNTFLAMFNYLLPFTNSLLKTSGAYATILSISAIGSIIGALIARKIKSSI
                     NSMLSMLVFSSLGVIVMGFPSLFELPIWISYSGSFLFNSLLTMFNIHFFSQVQIRVDE
                     AYMGRVMSTIFTIAIMFMPIGTLFMTIFSFALSNVSFIVIGCAIAILGGLGFSYSKKQ
                     F"
     gene            complement(4779..5632)
                     /locus_tag="VBE34_001786"
                     /pseudo
     CDS             complement(4779..5632)
                     /locus_tag="VBE34_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MutR family transcriptional regulator"
BASE COUNT         2074 a         1087 c          983 g         2018 t
ORIGIN      
        1 ttaacgattc tttttaaaat cattaaattt taaaacaaat ttcagacata ttgccaaagt
       61 tttgatatta ttactataat atagtttgta gaggagaata atatgggcca agaacctatc
      121 atcgaatatc aaaatatcaa taaagtgtat ggggaaaatg ttgcggttga agatattaac
      181 cttaaaattt accctggtga tttcgtttgt ttcatcggta cgagtggatc aggtaaaaca
      241 acattaatgc gtatggttaa ccatatgtta aaaccaacaa atggtactct attatttaag
      301 ggaaaagata tctctactat taaccccatt gaattaagac gcagaattgg atatgttatc
      361 caaaacattg gtttaatgcc tcatatgacc atttacgaaa atatagttct tgtaccaaaa
      421 ttattgaaat ggtcagaaga agctaaaaga gctaaagcaa gggaacttat taaattagtt
      481 gaattacccg aagaatattt ggatcgctac cctagtgagt tgtctggcgg tcagcaacaa
      541 cgtatcggtg tcattcgcgc tcttgcagca gaccaagata ttattttaat ggatgagcct
      601 tttggagctc tggatcctat tactagagaa ggtattcaag acttagtcaa gtctcttcag
      661 gaagaaatgg ggaaaactat catcttagtt actcatgata tggatgaagc cctcaagtta
      721 gcaacaaaaa ttattgttat ggacaatggt aaaatggtcc aagaagggac acccaatgat
      781 ctcttacatc atcctgctac tagtttcgtt gaacaaatga ttggagaaga gcgtctcctt
      841 catgcgcagg ctgatattac tcctgttaaa cagataatgt taaataatcc tgtttcaata
      901 actactgaaa aaacactaac tgaagctatt acactaatgc gccaaaaacg cgttgactca
      961 cttctagtaa ccgataacgg taaattaatt ggttttattg acttagaatc tctaagcagt
     1021 aaatataaga aagatcgact tgtttctgat atcttaaaac atactgattt ttatgttatg
     1081 gaagacgact tacttagaaa tactgctgag cgtattttaa aacgtggttt aaaatacgct
     1141 ccagttgttg accatgagaa taacttaaag ggcattgtta ctcgtgcatc cctagttgat
     1201 atgttatacg atattatttg gggcgatact gaaacggagg atcaataatg gtcaatttct
     1261 tatcacagta tggcatgcaa atattagtaa aaacatggga acaagtctat atctcatttt
     1321 ttgctatagc acttgggatt gcaattgctg ttccattggg tgtggtctta acacgttttc
     1381 ccaaagtagc caaaattatc attgctatag ctagcatgct acaaactata ccaagtttag
     1441 ccttattagc tttaatgatt ccattgtttg gaattggaaa agtcccagct atcgttgctt
     1501 tatttattta ttccttgctt cctatactaa ggaatactta tataggtatg aataatgtca
     1561 atccaacact taaagattgt gctaaaggga tgggaatgaa acctattcaa tctatatttc
     1621 aagttgagtt acctttagct actccaatta ttatggctgg tattcgcttg tctactattt
     1681 atgttattgc ttgggctaca ttagcttctt atataggtgc aggtggactt ggtgatctca
     1741 tttttagcgg gttaaatctt tttcagccca aattaattct agggggcaca attcctgtta
     1801 ttatcctgtc acttattata gattatcttt taggattact ggaaacagct ctaacgccca
     1861 gaacaacaag aagggaggct tagatatgct taaaaaatcg cactttttac agatatttac
     1921 actttgctta gccctcttaa cgatttctgg ttgtcaatta accgatacta aaaaacctgg
     1981 tcataccaca attaaggttg ctgcccaaag ttctacagag tctagtatca tggcaaatat
     2041 tgtcaccgaa ttaattcatc acgaattagg atacaacaca actttaataa gcaatcttgg
     2101 ttcctctacg gttactcacc aagctttgct ccgtggtgat gctgacattg ctgccacacg
     2161 ttatacagga acagacatca caggaactct tggcttaaaa gctgttaaag accctaaaga
     2221 agcttctaag attgtaaaaa ctgaattcca aaaacgctac aatcaaactt ggtatcctac
     2281 ttatggtttt tctgatactt atgcattcat ggttactaaa gagtttgcca gacagaataa
     2341 aatcaccaag atctctgatc tcaaaaagtt atcaacaact atgaaggcag gggttgatag
     2401 ttcatggatg aatcgcgagg gagatggata cactgatttc gctaaaacat acggttttga
     2461 attttcacat atttacccta tgcaaattgg cttagtctat gatgcggttg aaagtaacaa
     2521 aatgcaatct gtattaggct actccactga cggtcgtatt tcgagctatg atttagaaat
     2581 tttaagggat gataaaaaat tctttcctcc ttatgaagcc tctatggttg tcaacaattc
     2641 tatcatcaaa aaagatccta aactaaaaaa attactccat cgactcgatg gtaaaatcaa
     2701 tttaaaaacg atgcaaaacc ttaattatat ggtagatgat aaacttttag aaccttcagt
     2761 tgttgccaaa caatttttag aaaaaaacca ttattttaga ggagataaat aaatgaaaca
     2821 aatgaatact ttccaacaat ttatctacta cttccaacat aatggtagtt atatcttaga
     2881 acaatttatc caccactttt taatttcaat ttacggcgtt attttagctg caattgttgg
     2941 tatccctcta ggtatcttca tcgctagacg tggtaaaatt aaaacctttg ttatgggaat
     3001 cgcaaatgtc atccaaacca ttccttcttt agctatgatc tctatcatta tgttaggtct
     3061 agggctaggt attaaaacag tagttgcaac tgtctttctt tattctcttc tgccaatcat
     3121 cacaaatact tacactggta ttagaaatgt tgactccgac ttactagatg ctgctaaagg
     3181 aatgggcatg actaagcgtc aacgtttatt tatggttgag ttaccactat ctatttctgt
     3241 tatcatggct ggacttagaa atgcccttgt tgtagcaatt ggtattacag ccattggtgc
     3301 tttcgtcggt ggaggcggac ttggagatat catcataaga ggaactaatg caacaaatgg
     3361 tggtgctatt atcttagcag gatctcttcc aacagccctt atggcaattt tctctgattt
     3421 aatcctaggc ggtatccaac gcatgcttga accaagaaaa taactattaa aaacttgtca
     3481 gtagagacaa gtttttttga tgtttcagaa taatttctta agttctaaaa ttgtttttta
     3541 ctatagctaa atcctaatcc tcccaatata gcaatggcac atcctataac aataaagcta
     3601 acatttgata aggcaaatga aaagatagtc ataaatagtg ttcctatagg cataaacata
     3661 atagcaatag taaatatagt cgacatcaca cgccccatat acgcttcatc gaccctgatt
     3721 tgtacctgac tgaaaaaatg aatattaaac attgtcagaa gactattaaa aagaaaacta
     3781 ccactgtaag atatccatat cggtaattca aatagagatg ggaatcccat aactatgact
     3841 cccaaactac tgaatactag catagatagc atagaattaa tactagattt aatttttcta
     3901 gcaataagcg ctccaataat tgatccgata gctgatatac taagtattgt tgcataagca
     3961 cctgaagttt tcaataggga gttagtaaaa ggtaaaagat aattgaacat tgctaggaaa
     4021 gtgtttaata aagcagcaat aattagaaaa aacataattt ctttttcttt tttgatgtaa
     4081 gtaaatccat ctgcaatatc agctaaaata ttttttatcg ctaccttttc ttgttttgaa
     4141 agctgtaccc tttccacttt aatagcataa agaaacagga aagagatcaa aaaagtaatc
     4201 gcatcaacaa ttaaagtaat cctaatacca aaattattaa aaataaggaa acctaaaaca
     4261 ggggaactta cactaataat ttgcacaatg gtttccagat tagcattata ggtaatgata
     4321 tcagctttat ctactatttc aggaatataa gatttataag ccggagaaga aaaagcattg
     4381 gaaaccgcta agatagcatt agcaactatt agaccataca ccattacttt atcatctcca
     4441 ataaaagaaa gtaagaaaca catgatagca cagatagcat ctgttattag taaaattttt
     4501 cttcgttgaa agcgatcagc taaggcgcct ccaaaagggt tgaggacaat agaaaccaac
     4561 aactctacta tctgataaat acctaaaatc ttttgtccta gaccacctat cgaagcaatc
     4621 caagtactat ttccataatc ataaagcaca tcaccaagtt tactgactgc tcgactagat
     4681 agtaatttta taccattaat attcataatt ccctccttag ttcgacatta tagcaaacca
     4741 aaaggagaaa tattaaaaaa tcccataaat gggatttttt ataatcttgt taccctatta
     4801 aagtgttcct gaaaattttc aacatgatgt ttagaaccaa ttgcttggaa gcaaaaaata
     4861 gcttgcttca tgctttgtat tcctcgacta tcccctttag cataggcaag aaatcctttt
     4921 tcatagagta aaataatacg gtgataagca ttccgctcat tatttaaaag tgcttcaact
     4981 ttctctagaa aaaacgttgc ttcttctaga gcgcgccgtt ctattagtgt ctctaccaca
     5041 tttaggaggg ttgcttcgac aacattgcgg tttgtataaa tatctcgata atattccttt
     5101 tgattgataa cctctttaac aagttggcaa atatgccttg tgttgtaata ccggtaaaga
     5161 tttccaatca aaatatagtc actaatttcc caactatcct tttgaaagag gtaatccgac
     5221 aaaaagcaaa ggtcttcttc cgacattttt cttgaggagt catattcaca tatcattcct
     5281 ctaaaaagaa ctatgtttaa acgacaacgt atatctgaac tatccttttt tagtttacct
     5341 tcttccacag aaatcatctt ttctaaccca gctacgtctc tttgataatg atattgagcc
     5401 atctgagaca ttactgatca gatttttgat atttatttac tctatccata aattcgctaa
     5461 ttgataaatc aattgcctct aaagccccta aaaattttgt taatgataaa tctgattcac
     5521 ccctctcaaa tcgtgataac tgagaaacag aaagttcatt tgaagcaacc tgtttaagag
     5581 aaaactgtcg attaagtcga aattccctaa aaatagggcc atatataaac attagtattc
     5641 ctttctaacc gtaatctcat tggtaaactt atgaggtaat ttcaataatt tatatttacc
     5701 aactttaacc aatatatgag ctgatatata gtcattttct tttatttgta atatcatatc
     5761 acaagttctt ctaaatcaac atatgttaat ttatctggga atttttctcg taaggttact
     5821 ttcatatatt gagtacttac catacaaaat tcctccatca tatactcttt ttttcaatta
     5881 taaattaaaa tagaatcatc tcatctaact ttgtcattgc ctttgtgcta tttaaagtag
     5941 ttatttaaat ttacaatggt ttctcaagta tttagaagtt ttaaaaatga ctttaggaca
     6001 atttttgaat attacacaaa aaacacccca tggtgtgaac catgaagtgc ttccaatcgc
     6061 tgatatatag gtatatatta acgttttgag gattgtgatg ctttacgggc tttttttaaa
     6121 cctggttttc tctgacaact gtaatatatg gtgttatatg gt
//
LOCUS       DATRUK010000082         6156 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.082, whole genome shotgun sequence.
ACCESSION   DATRUK010000082 DATRUK010000000
VERSION     DATRUK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.082"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            175..3792
                     /gene="srr2"
                     /locus_tag="VBE34_001787"
     CDS             175..3792
                     /gene="srr2"
                     /locus_tag="VBE34_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086874280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin Srr2"
                     /protein_id="HEO7264476.1"
                     /translation="MLKKQFGNFGEKSRKVRVKMRKSGKHWVKSVMTQIGYVILSRFS
                     GKEKSSKVQTTSEDLSRTKTSASILTAVAALGAVVGGTTDTTSVSAEETPTATELTGN
                     EKTLATAETVVVAPEVKTVNSDSSSHSTSESQSMSTSTLQSTSASLSASESLMDSTSA
                     SLSESSSLSEYSSLSLSSSESVSASESVQSSEAATTARVQPRAMRVVSSASDMETLPA
                     ALISGEGDVTTVQGQDVTDKLQNLDIKLSGGVQAKAGVINMDKSESMHMSLKFTIDSV
                     NRGDTFEIKLSDNIDTNGASNYSIVEPIKSPTGEVYATGIYDSQKKSIVYSFTDFAAS
                     KNNINGILDIPLWPDDTTVQNTKEDVLFSVKIKDQEATIKETVKYDPPVRIDFAGGVS
                     VDSRITNIDDVGKKMTYISQINVDGKSLYNYNGLYTRIYNYSKESTADLKNSTIKIYK
                     TTSDNIVESMVQDYSSMEDVTSKFANSYPEKGWYDIYWGQFIASNETYVIVVETPFTN
                     AVTLNTTLSDYNENNGVEHNHTYSSESGYSDVNAQERKILSELVSSSESVSSSESVSN
                     SESISTSESVSNSESISSSESVSSSESISTSESVSTSESISSSESVSSSESVSSSESI
                     SSSESVSNSESISSSESVSNSESISSSESVSSSESISNSESISSSESVSTSESISSSE
                     SVSNSESISSSESVSSSESISNSESISSSESVSTSESISNSESVSSSESVSTSESISS
                     SESVSNSESISTSESVSTSESISSSESVSSSESISSSESVSNSESISNSESVSSSESV
                     SNSESISSSESVSNSESISTSESVSTSESISSSESVSNSESISSSESVSNSESISSSE
                     SVSNSESISSSESVSNSESISSSESVSSSESVSSSESISTSESVSNSESISSSESVSN
                     SESISSSESVSNSESISSSESVSNSESISSSESVSSSESISSSESVSSSESVSNSESI
                     SSSESVSNSESISSSESVSTSESISSSESVSNSESISSSESVSSSESVSSSESISTSE
                     SVSNSECISSSKSVSNSESISSSESVSNSESISSSESVSNSESISSSESVSSSESISS
                     SESVSNSESILSSESVSSSESISSSESISSSESVSMSTTESLSESEVSGDSEISSSTE
                     SSSQSESMNHTEIKSDSESQHEVKHQVLPETGDNSASALGLLGAGLLLGATKSRKKKK
                     D"
     gene            3902..5155
                     /gene="secY2"
                     /locus_tag="VBE34_001788"
     CDS             3902..5155
                     /gene="secY2"
                     /locus_tag="VBE34_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEO7264477.1"
                     /translation="MIGKLYYSYRKSTLLRSILWTILIVGVYMLGQRVLLSTVPLSHQ
                     EIKLAVDQHLLNNFSAVSGGSFNKLNVFTLGLSPWMSSMIIWRFVSLFSWAKNATKRK
                     AEVAQYTLMLTISVIQAYGVSGNQFIKSSLLGSYSDIVFKALTMVLLISGSFVLMWLA
                     NLNASKGLGGSIVIILVSMLQATTTGIITYFEQSHFSIMDLLGTIIAISIILSVLLLI
                     STVVYRAEYRIPIRRIDIVSKFAQDTYIPIRINPAGSYPFMYGMTLMMLPTFIIQALL
                     LAFPNERLLIQISSMIAINKPLGIGLFVILLFILSIGFAYFNYDPYNIAKNMRQRGEY
                     FQNVTPGAATQRFLQEKISIMAVVGAIFTVIMGGLPMLAVVGKTNQVSIAMIVINIYI
                     ISTFMLSIIEQVNTLKLWGSHRNII"
     gene            5165..>6156
                     /gene="asp1"
                     /locus_tag="VBE34_001789"
     CDS             5165..>6156
                     /gene="asp1"
                     /locus_tag="VBE34_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEO7264478.1"
                     /translation="MFYFIPSWYNSDQKWHNEAPIWYRVLENMTFDDTVSQVRMFQNA
                     DEEVRLLLFSYNPQLRYMLHEESMLAVSYWSFFDDIQNIQQKEIKLINFKELNWPKGT
                     YFVYTPFLVLAKHGGEIIARIQFAENGNLLLIELLIDGVLDKIMIFDDRGFVSSIKYF
                     KQNRLIYQDYLNQNGVWQIREYASKFPRIVVNPMSDKVFNFTEYDDWDTLIRERILVL
                     KKLEVTPDDYIIVAVNDIHNNLVRKSFPDQKKIFSFFGERYSFDNRLDFNELLDEGEL
                     FIAESEKIERLLITSLQEKNYSSNYVENHISRIPPFDTRLRLGQSQNIEELIAY"
BASE COUNT         2013 a         1007 c         1132 g         2004 t
ORIGIN      
        1 gctttttaag ataggagggg tctttatagg caaatatgct atttattgat gatgtttcaa
       61 gcaatatttt agaacttgaa ataacaaagt agtattttgt tatagctata tttgagaatt
      121 cttagaatta gaaaagtttt ttatatagca ttatttttta aatatggagg aaaaatgtta
      181 aaaaagcagt ttggaaactt tggtgaaaaa tcacgcaaag ttcgagttaa aatgaggaaa
      241 tcaggtaagc actgggtcaa aagtgttatg acacaaattg gctatgttat cctttcgagg
      301 tttagcggta aagagaaaag ctctaaggtt caaactacta gtgaagattt aagcagaact
      361 aaaacgtctg ctagcatcct aacagcagtc gcagctcttg gagctgtagt tggagggacg
      421 acagacacaa cttctgtctc agctgaagaa acacctactg caacagaact tacaggaaat
      481 gaaaaaacat tagctactgc tgaaactgta gtggttgcgc cagaagtaaa aactgtaaac
      541 tcagattctt catcacattc aacaagtgaa tcacaatcaa tgtcaactag taccttgcaa
      601 tctaccagcg cctcattatc tgccagtgaa tcactaatgg attctacctc tgcttcttta
      661 tctgagtcta gctcgttatc agaatatagt tctttatctc tatctagtag tgagagtgtc
      721 tcagcatcag aatcagttca atcatcagaa gcggcaacga ccgctagagt tcaaccgaga
      781 gcgatgagag ttgtatctag tgcttcagat atggaaactt taccagcagc attaatctct
      841 ggtgaaggag atgtaacaac tgttcaagga caagatgtaa cagataagtt acaaaattta
      901 gatataaagc tctctggagg agtgcaagca aaagcaggtg ttataaatat ggataagtca
      961 gaaagtatgc acatgtcttt aaaattcact attgattctg tgaatagagg tgatactttt
     1021 gaaattaagt tatcagataa tatcgataca aatggagctt ctaactattc tattgtagaa
     1081 cctataaaat cacctacggg tgaggtctat gcgactggaa tttatgattc tcaaaaaaaa
     1141 tctatagtct atagttttac agattttgcg gcttcaaaaa ataatattaa tggaatatta
     1201 gatattccat tatggccaga tgatacgaca gttcaaaaca caaaagaaga tgttcttttt
     1261 tcggtaaaaa taaaggatca agaggctaca attaaagaaa cagtgaaata tgatccaccg
     1321 gtaagaattg attttgcagg gggagtaagt gtagattctc gaataaccaa cattgatgat
     1381 gtgggaaaaa agatgactta tataagtcaa attaatgtag atggaaaatc actctataat
     1441 tacaacgggt tatatactag gatatataat tatagcaaag agagtacagc agatttaaag
     1501 aattcaacga taaaaatcta taaaaccacc tcggataata ttgtagagag tatggtacag
     1561 gattattcaa gcatggagga tgtaactagt aagtttgcaa atagttaccc agaaaaaggg
     1621 tggtatgata tttattgggg gcagtttatt gcatcaaatg aaacgtatgt aattgttgta
     1681 gaaacaccat ttactaatgc agtaactttg aatactactt tatcagatta taatgagaac
     1741 aatggtgtag aacataatca tacttactca tctgaatcgg gatattcaga tgtaaatgct
     1801 caagaaagaa aaattttatc cgaattagta agtagctcag aatcagtatc aagttcagaa
     1861 tcggtatcta attccgaatc tatttcaact tctgaatcgg tatctaactc cgagtctatt
     1921 tcaagttccg aatcggtatc aagctcagaa tctatttcaa cttctgaatc ggtatcaact
     1981 tcagagtcta tttcaagctc cgagtcagta tcaagttcag aatcggtatc aagctcagaa
     2041 tctatttcaa gttccgaatc ggtatctaac tccgagtcta tttcaagctc cgaatcggta
     2101 tctaactccg aatccatttc aagttctgaa tcggtatcaa gctcagaatc tatttcgaat
     2161 tcagagtcta tttcaagctc cgagtcagta tcaacttcag agtctatttc aagctccgaa
     2221 tcggtatcta actccgaatc catttcaagt tctgaatcgg tatcaagctc agaatctatt
     2281 tcgaattcag agtctatttc aagctccgag tcagtatcaa cttcagagtc tatttcaaac
     2341 tccgagtcgg tatctagctc cgagtcagta tcaacttcag agtctatttc aagttccgaa
     2401 tcggtatcta actccgaatc catttcaact tccgagtcag tatctacctc ggaatctatt
     2461 tcaagttctg aatcggtatc aagctcagaa tctatttcaa gttccgaatc ggtatcaaac
     2521 tccgagtcta tttcaaactc cgagtcggta tctagctccg agtcagtatc aaattcagag
     2581 tctatttcaa gttccgaatc ggtatctaac tccgaatcca tttcaacttc cgagtcagta
     2641 tctacctcgg aatctatttc aagttctgaa tcggtatcaa attcagagtc tatttcaagt
     2701 tccgaatcgg tatctaactc cgagtctatt tcaagttcag aatcggtatc aaattcagag
     2761 tctatttcaa gttccgaatc ggtatcaaat tcagagtcta tttcaagttc agagtcagta
     2821 tcaagttcag agtcagtatc aagctcggaa tctatttcaa cttcagaatc ggtatctaac
     2881 tccgagtcta tttcaagttc cgaatcggta tctaattctg aatctatttc aagttccgaa
     2941 tcggtatcaa attcagagtc tatttcaagt tccgaatcgg tatcaaactc cgagtctatt
     3001 tcaagttccg agtcagtatc aagctcagaa tctatttcaa gttccgaatc ggtatctagc
     3061 tccgagtcag tatcaaattc agagtctatt tcaagttccg aatcggtatc taactccgag
     3121 tctatttcaa gttcagaatc ggtatcaact tcagagtcta tttcaagttc cgaatcggta
     3181 tcaaattcag agtctatttc aagttcagag tcagtatcaa gttcagagtc agtatcaagc
     3241 tcggaatcta tttcaacttc cgaatcggta tctaactccg agtgtatttc aagttccaaa
     3301 tcggtatcta attctgaatc tatttcaagt tccgaatcgg tatcaaattc agagtctatt
     3361 tcaagttccg aatcggtatc aaactccgag tctatttcaa gttccgagtc agtatcaagc
     3421 tcagaatcta tttcaagttc agaatcggta tcaaactccg agtctatttt aagttccgaa
     3481 tcagtatcaa gctcagaatc tatttcaagt tcagaatcta tttcaagttc agagtcagta
     3541 tcaatgagta ctacagagtc tctaagtgaa tcagaagtat caggggattc tgaaattagc
     3601 tcaagtacag aatcatcaag tcaatctgaa tcgatgaatc atactgaaat taaatccgat
     3661 tccgaatctc aacacgaagt taagcatcaa gtattaccag aaacaggtga taactcggct
     3721 tcagcattag gtctgttagg tgcaggattg ttgttaggag ctactaaatc tcgcaagaag
     3781 aaaaaagatt aattttcaaa gtgatattta gatgataaat cgaaagtact ttcgatttat
     3841 cacttaagat agtaagtagt tatactgtct taagtgataa attgatttga aaagggaaaa
     3901 aatgattgga aaattatatt atagctatag aaagtcaacg ctattaagaa gtattttatg
     3961 gactatttta attgttggtg tatatatgtt aggacaacgt gttttattat ccactgttcc
     4021 tttatcacat caagagataa aactagcagt agatcaacat ttactcaata acttttcagc
     4081 agtaagtggt gggagtttta ataaattaaa tgttttcaca ctggggttga gtccatggat
     4141 gtcaagtatg attatttgga gattcgtttc cttattttcg tgggcaaaaa atgcaacgaa
     4201 gcgaaaagca gaagtagctc aatatacttt aatgcttact atctcagtta tacaagcata
     4261 tggtgtttca ggaaatcaat ttataaaaag ctctttatta ggttcttata gtgatattgt
     4321 ttttaaagca ttgacaatgg tcttgttaat aagtggctca tttgtgttaa tgtggcttgc
     4381 taatctcaat gcttctaaag ggctaggagg aagtattgtt atcatattag tgagcatgct
     4441 ccaagcaacg actactggaa taataactta ttttgaacag tcacatttta gtattatgga
     4501 tctgctggga actattatag ctataagtat aatacttagt gttttattat taatctcaac
     4561 ggtggtttat agggcagaat atcgtatacc tattaggcgt attgatatcg ttagtaaatt
     4621 tgcccaagat acctatattc ctattcgcat aaatccagca gggtcctacc ctttcatgta
     4681 tgggatgaca ttaatgatgc ttccaacgtt tataattcaa gcattgttac ttgcttttcc
     4741 aaacgagcga ttattgattc agatctcgtc aatgattgca attaataagc ctttaggtat
     4801 tggacttttt gttatattat tatttatttt gagcattgga tttgcatatt ttaattacga
     4861 cccgtataat attgcaaaaa atatgcgtca acggggggaa tattttcaaa atgtaactcc
     4921 aggagctgct actcaacgtt ttttacaaga aaaaattagt atcatggcag tagttggagc
     4981 aatttttacg gttattatgg gaggtctccc aatgttggct gtggtgggga agacaaacca
     5041 agttagtatt gcaatgatcg tcattaatat ttatattatt tcaactttta tgctcagtat
     5101 tattgagcaa gttaatacct tgaagttgtg gggcagtcac cgaaatatta tttaaggagc
     5161 aatcatgttt tattttattc cttcgtggta caattctgat caaaagtggc ataatgaagc
     5221 tcctatctgg tatcgtgtat tggagaacat gacttttgat gatactgtta gtcaggttag
     5281 aatgtttcag aatgcagatg aggaagtgcg tttacttcta tttagttata atcctcagtt
     5341 gagatacatg cttcatgaag agagtatgtt ggctgtctct tattggtctt tttttgacga
     5401 tattcaaaat attcaacaaa aagagattaa gttaattaat tttaaagaac tgaattggcc
     5461 taaagggact tattttgttt atacaccttt tttagtatta gctaaacatg gtggcgagat
     5521 tattgcacgt atccaatttg cggaaaatgg gaatttacta ctgattgaac ttttaataga
     5581 tggtgtgctt gataagatta tgatatttga cgatcgtggt tttgtatcca gtatcaaata
     5641 tttcaaacaa aatagattaa tttatcaaga ttatttaaat caaaatggtg tttggcaaat
     5701 tcgagagtat gcttcaaaat ttccgagaat agtagttaat ccaatgtctg ataaggtgtt
     5761 taattttact gagtatgatg attgggatac tcttattcga gagagaatat tggttttaaa
     5821 aaaactagaa gtgactccag atgattatat tatagtagca gtaaacgata ttcataacaa
     5881 tttagtcaga aaatcatttc cagatcaaaa gaaaattttc tcattttttg gtgaacgcta
     5941 ttcatttgat aatcgactcg actttaatga acttcttgat gaaggtgaat tgtttattgc
     6001 tgaaagcgaa aaaatagaac ggttattgat tacttcatta caagagaaaa attattcgtc
     6061 aaactatgtt gagaatcaca ttagccgtat tccccctttt gatactcgtt tgagactcgg
     6121 acagagccaa aatattgaag agcttattgc atattt
//
LOCUS       DATRUK010000083         6077 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.083, whole genome shotgun sequence.
ACCESSION   DATRUK010000083 DATRUK010000000
VERSION     DATRUK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.083"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(19..1800)
                     /gene="uvrC"
                     /locus_tag="VBE34_001790"
     CDS             complement(19..1800)
                     /gene="uvrC"
                     /locus_tag="VBE34_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEO7264479.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKNGTIIYVGKAKNLKNRVKSY
                     FHGSHNTKTELLVSEIEDFEYIVTTSNTEALLLEINLIQENMPKYNIRLKDDKSYPYI
                     KITNERYPRLMITRQVKKSDGTYFGPYPDSGAATEIKRLLDRLFPFKKCTNPANKVCF
                     YYHLGQCNAHTVCQTNKAYWDSLREDVKQFLNGKDNKIVNGLTEKMKSAAMTMEFERA
                     AEYRDLIEAISLLRTKQRVIHQDMKDRDVFGYFVDKGWMCVQVFFVRNGKLIQRDVNM
                     FPYYNEPEEDFLTYIGQFYQDTKHFLPKEVFIPQDIDAKSVETIVGCKIVKPQRGEKK
                     QLVNLAIKNARVSLQQKFDLLEKDIRKTHGAIENLGNLLNIPKPVRIEAFDNSNIQGT
                     SPVAAMVVFVNGNPSKKDYRKFKIKTVIGPDDYASMREVIHRRYSRVLKDGLTPPDLI
                     VIDGGQGQVNIARDVIENQLGLAIPIAGLQKNDKHQTHELLFGDPLEVVELPRNSEEF
                     FLLHRIQDEVHRFAITFHRQLRSKNSFSSKLDGITGLGPKRKQLLMKHFKSLPNIQKA
                     DIEDIIMCGIPRTVAESLRDSLNDPPK"
     gene            1959..2726
                     /locus_tag="VBE34_001791"
     CDS             1959..2726
                     /locus_tag="VBE34_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEO7264480.1"
                     /translation="MIDVENILEKMKPNQKINYDWVMQQMVKQWQASDIRPKILMHVC
                     CAPCSTYTLEYLSQWADVTIYFANSNIHPKDEYYRREYVTQKFVHDFNKNTGYSVQFL
                     SAPYEPNEFFKIVHGLEEEPEGGDRCKVCYDFRLDKTAEKAVELGFDYFGSALTISPH
                     KNSQTINTIGIDVQKIYDTQYLPSDLKKNKGYQRSVEMCKDYDIYRQCYCGCIFGAKD
                     QGINLLQIKKDAKAFVSDKDGKEEFPNIRFTFNGKSM"
     gene            complement(2785..4125)
                     /locus_tag="VBE34_001792"
     CDS             complement(2785..4125)
                     /locus_tag="VBE34_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO7264481.1"
                     /translation="MYQTQTNKEKFVLFLKLFIPVLIYQFANFSATFIDSVMTGQYSQ
                     LHLAGVSTASNLWTPFFALLVGMISALVPVVGQHLGRGNKEQIRTEFHQFLYLGLILS
                     LILFLIMQFIAQPVLGSLGLEDEVLAVGRGYLNYMLIGIMPLVLFSICRSFFDALGLT
                     RLSMYLMLLILPFNSFFNYMLIYGKFGMPRLGGAGAGLGTSLTYWAIFIVIIIVMSLH
                     PQIKTYHIWTLERIKAPLIIEDIRLGLPIGLQIFAEVAIFAVVGLFMAKFSSIIIAAH
                     QAAMNFSSLMYAFPLSISTALAITISFEVGAERFQDANTYSRIGRLTAVGITSGTLLF
                     LFLFRENVAAMYNSDPHFVAITAQFLTYSLFFQFADAYAAPVQGILRGYKDTTKPFMI
                     GAGSYWLCALPLAVILEKNSQLGPFAYWIGLITGIFVCGLFLNQRLQKIKKLYY"
     gene            complement(4225..4641)
                     /locus_tag="VBE34_001793"
     CDS             complement(4225..4641)
                     /locus_tag="VBE34_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7264482.1"
                     /translation="MVMKAIEIYMNINGRGAEAITFYEEAFNTKAEHVTTFAQGWPDC
                     PPELEHLIMNAQFNIDGIRMQLSDNNPIFDFQIGQNMTAALIVESVEEAQELFNKLSR
                     DAMNIKMPLQAVPWSPAYGNVTDKYGMTWQINTELS"
     gene            complement(4645..5142)
                     /locus_tag="VBE34_001794"
     CDS             complement(4645..5142)
                     /locus_tag="VBE34_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO7264483.1"
                     /translation="MIIDFKTVSTKGLETSPVAEALAGLRANEARYFWNKFKVAYETF
                     PVSDYPELMADINHLLKNERNLVFAAKPLEISQLFLENRILWTHVFYEDGLAINILYE
                     LDNPKKRAVGLKLSDGMDIPIELADKFKFARQKSKLAGIIRGSYFTLKGENETLMQRW
                     LDNMK"
     gene            5364..5960
                     /locus_tag="VBE34_001795"
     CDS             5364..5960
                     /locus_tag="VBE34_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEO7264484.1"
                     /translation="MTKKIFLFICATLLVGIALYTPTTSIFNHNNVYAATKKAKIKFN
                     KTQKKIVKKAREYAKSGHMSKDSIIEKLKKDSKKYRQEDINFVINNLKVDYKKNALIS
                     AKIYSKTMNLSKQSIFEQLYSESPDKATHSDKFTKEESQYAIDHLKVDFKENALETAK
                     SYQSSSSLSKEEIYKQLTSTLGDKFTNDEAQYAVDHLK"
BASE COUNT         2098 a         1150 c          903 g         1926 t
ORIGIN      
        1 actgttctac taagtttttt atttaggagg atcattaaga gaatctcgga gactttctgc
       61 aactgtccta ggaataccac acataataat gtcctcaata tcagcttttt ggatattagg
      121 taagctttta aaatgtttca ttagcaattg tttacgtttt ggtcccaacc cagtgatacc
      181 atctaatttt gaagaaaagg aatttttgga gcgtaattga cgatgaaaag taatagcaaa
      241 acgatgtacc tcatcttgaa tacggtgaag taagaaaaat tcttctgaat tacgcggtaa
      301 ttctactact tctaaaggat ctccaaaaag taattcatgt gtttggtgtt tatcattctt
      361 ttgcaaacct gcaataggta tggctaaacc taattggttc tcaataacat cacgcgctat
      421 atttacttgc ccctgaccac catcaattac aatcaagtcc ggaggtgtca agccatcttt
      481 taaaacccta ctataacgcc gatgaatgac ttctctcata ctagcataat catctggtcc
      541 gataacagtc ttaattttaa actttcgata gtctttttta cttggatttc cattcacaaa
      601 taccaccata gcagctactg gacttgtccc ttgaatattg gaattatcaa atgcttctat
      661 gcgtactggt ttagggatat taagaagatt acctaaattt tcaattgccc catgagtttt
      721 acgaatatct ttttccagta agtcaaactt ctgttgtaaa ctgacacgag cattttttat
      781 tgccagattt actaactgtt tcttttcacc acgttgtggt ttaacaatct tacagcctac
      841 aattgtttca accgacttag catcaatatc ctgtggaata aagacttctt tgggtagaaa
      901 atgttttgta tcttgataaa attgccctat gtaagttaga aaatcttcct ctggttcatt
      961 ataataagga aacatattca cgtcacgctg aatcaatttt ccatttcgaa caaaaaagac
     1021 ctgtacacac atccaaccct tatcaacgaa ataaccaaaa acatctctat ccttcatatc
     1081 ttgatgaata acacgctgct tagtacgcaa caaactaatt gcttcgatca aatctcgata
     1141 ttcagccgca cgctcaaatt ccatagtcat agcagcgctc ttcatttttt cagttaatcc
     1201 gttaacaatt ttattatctt taccattaag aaactgtttt acatcttctc tcagactatc
     1261 ccaataagct ttattagttt gacaaactgt atgtgcattg cattgcccta aatgataata
     1321 aaagcatact ttatttgctg gattagtaca tttcttaaaa ggaaagagcc tatctagtaa
     1381 ccgcttaatc tcagttgctg ccccagaatc cggataaggt ccaaaataag tgccatctga
     1441 tttttttact tgacgtgtaa tcataagacg aggataacgt tcatttgtta ttttgatata
     1501 tgggtaagac ttatcatctt tcaatcgaat attatacttc ggcatatttt cttggattaa
     1561 attaatttcc aataatagag cttcggtatt agatgttgtc acaatgtatt caaagtcttc
     1621 aatctcagag accaaaagtt ctgtcttagt attatgactc ccatgaaaat aacttttcac
     1681 acgattctta agatttttag ccttacctac ataaataatt gtcccattct tatctttatg
     1741 aaggtaacaa ccaggactgt ctggtaacaa ttctagctta tgtttaatca gttcattcat
     1801 agaactatta taacaaaatg tactgtaatc tttacgaaat aaatactatt ccccaacatc
     1861 tattgtatct tccaatactt gaataaaacc ccaatactcc tagtctaagc aattatttta
     1921 tgttacactt taaacaagtg aattattgag gagtttttat gatagacgtt gagaatattc
     1981 ttgaaaaaat gaaacctaac caaaaaatca attacgattg ggtcatgcaa caaatggtta
     2041 agcagtggca agctagtgat atccgtccga aaattctgat gcatgtttgc tgtgctcctt
     2101 gtagtacata tacccttgaa tacttatcac aatgggcaga tgttaccatc tattttgcta
     2161 attcaaatat ccatcctaaa gatgaatatt atcgccgcga atatgttact caaaaatttg
     2221 ttcatgattt caacaaaaat actggatatt ctgtacaatt cttatcagca ccatatgaac
     2281 caaatgaatt tttcaaaatt gttcatgggt tagaagaaga acctgaaggt ggggatcgtt
     2341 gtaaagtttg ctatgatttc cgtcttgata agacagcgga aaaagccgtt gaactaggtt
     2401 ttgattactt tggcagtgcc ctaactatta gtcctcacaa aaactctcaa acaatcaata
     2461 ctattggtat tgatgtccaa aaaatttatg atactcagta tcttcctagc gaccttaaaa
     2521 aaaataaagg ttaccaacgt tccgttgaga tgtgtaaaga ttatgatatc taccgacaat
     2581 gttactgtgg gtgtatcttc ggtgctaaag atcaagggat aaatcttctc caaatcaaaa
     2641 aagatgcgaa agctttcgtg agtgataaag acggcaaaga agaatttcct aatatacgct
     2701 tcacatttaa tggcaagtca atgtaaatga taaaaaaaga cttagtaaag aactaagcct
     2761 ttttggtata aatcttgtca aactttaata atacaacttc ttaatctttt gcagacgttg
     2821 gtttagaaat agaccacaaa caaaaatacc tgtgattaaa ccaatccaat aggcaaacgg
     2881 acctaactgg ctattttttt ctaagataac cgctaatggc aaagcacata accaataaga
     2941 gcccgcaccg atcataaatg gttttgttgt atccttatag cctcgtaaaa tcccctgtac
     3001 aggagctgca taagcatctg caaactggaa aaagagacta taagttagga attgagctgt
     3061 aatagcgaca aagtgagggt cactattata cattgctgct acattctcac gaaatagaaa
     3121 taaaaatagt aaggttcctg atgtaatccc taccgctgtt aagcgtccta tcctactata
     3181 agtgtttgcg tcctgaaagc gctctgcccc tacttcaaac gatattgtaa tagctagagc
     3241 agtggaaata cttaaaggaa atgcatacat taatgatgaa aaattcatag cagcctgatg
     3301 tgctgcaata atgattgaag aaaattttgc catgaataag cctactactg caaaaattgc
     3361 aacttctgca aaaatttgta aaccaatcgg taatcccaat cgaatatctt caataatcaa
     3421 aggagctttt attctttcca gagtccatat atgatatgtt ttaatttgag gatgaagtga
     3481 catcacaata ataataacaa taaaaatagc ccaataagtt aaagaagttc caagacctgc
     3541 ccccgcacct cctagtctag gcataccaaa tttaccgtag ataagcatat aattaaaaaa
     3601 tgaattaaag ggtagaatta aaagcatcag atacatagat aaccttgtta accccaatgc
     3661 atcaaagaat gaacggcaaa tgctaaacaa caccagcggc atgattccaa tcaacatata
     3721 atttaaataa ccacgaccaa ctgctagaac ttcatcttct aaacccaaac tccccaagac
     3781 aggttgagca ataaattgca tgattaaaaa taatattaag gacagtatca aacctaaata
     3841 tagaaattga tgaaattctg tgcgaatttg ttctttattt cctctaccca aatgttgacc
     3901 aactactggt actaatgctg aaatcatacc tactaataaa gcgaaaaacg gagtccataa
     3961 attactagca gttgacacac ctgccaaatg tagctgacta tactgtccag tcataaccga
     4021 atcaataaaa gtagctgaaa aattagcaaa ttgataaatc aatactggga taaataattt
     4081 caaaaataaa acaaattttt ccttatttgt ctgagtttga tacatctttt ttctcctttt
     4141 caccttaatc aatttatttt agcttagcta ggatactctg tcaatgctat ttaaaaaggt
     4201 tgagaaataa tcctcaaccc cattttagct aagctcagta ttgatttgcc atgtcatacc
     4261 atatttgtca gtcacatttc cataagctgg actccacgga actgcttgta gaggcatttt
     4321 aatgttcatt gcatctcgag aaagcttatt gaataactct tgcgcttctt ctacagattc
     4381 cacaattaaa gctgctgtca tattttgtcc aatttgaaaa tcaaaaatag gattattgtc
     4441 agaaagttgc attcgaatac catcaatatt aaattgagcg ttcataatta aatgttcaag
     4501 ttctggcgga caatctggcc aaccctgtgc aaaagttgtg acgtgttctg cttttgtatt
     4561 aaaagcctct tcataaaatg taatagcttc ggccccacga ccattaatat tcatataaat
     4621 ttcaattgct ttcattacca tacctcactt catattatcc aaccaacgct gcatcagagt
     4681 ttcattttcc ccttttaggg taaaataaga accacgtatt atacctgcga gttttgactt
     4741 ttgacgagca aacttaaact tgtccgctaa ttcaatagga atatccattc catctgataa
     4801 ttttaaacct acagctctct ttttaggatt atctaattca tataaaatat taatggctaa
     4861 accatcctca tagaaaacat gtgtccataa aatacgattc tctaaaaaaa gctgtgaaat
     4921 ctctaaaggc tttgcagcaa aaacaaggtt acgttcattt tttagaaggt gattaatatc
     4981 tgccatcaat tctggatagt ctgatactgg aaatgtttcg taggctactt tgaatttatt
     5041 ccagaaatag cgagcttcat ttgcacgcaa accagccaag gcttctgcca ctggagaagt
     5101 ctccaaacct ttagttgaaa cggttttaaa gtcgataatc atgttctttc ctttttgatt
     5161 aaaatcttcg ataatttagt atactctttt ttgaagaaaa agtacattct aatcacttat
     5221 aaaaagtagt acatttagaa cactaccacc tcagtaattt cttgaccttt ctctacttaa
     5281 tacttataat taatctagac ttaatgtcaa agcatcaatc aaggtgtaaa cctgattcac
     5341 ctaaaaagct gatggagcta aacatgacca aaaagatatt cttatttatc tgcgctactc
     5401 ttttagtagg aatagcctta tatacaccaa caacttctat ttttaatcat aacaacgttt
     5461 atgctgccac caagaaagca aaaattaaat ttaataaaac tcaaaaaaaa attgtcaaaa
     5521 aagcaagaga atatgctaag tctggccata tgtctaaaga tagtatcatt gaaaaattaa
     5581 aaaaggattc taagaaatat cgtcaagaag acatcaactt tgtgattaat aacctcaagg
     5641 ttgattacaa aaaaaatgcc ttgatttctg ccaaaattta ttcaaagacc atgaatttat
     5701 ctaaacaatc tatctttgaa cagctctatt cggagtctcc agataaggca acccactctg
     5761 ataaatttac aaaagaagaa tcccaatatg ctatagacca tctcaaagta gatttcaaag
     5821 aaaatgctct tgagactgct aaaagttatc aaagtagttc ttctttatcc aaagaagaaa
     5881 tctataaaca attgacatct acgctgggcg ataaattcac aaatgatgaa gcccaatatg
     5941 ctgtagatca tttgaaatga tgacatacca atagtctatc cttatgtaat agaattgata
     6001 gcttatgcta atattccagc tgtaaaatag caagaaccct cctaagttca ttaagactta
     6061 ggagggtttt tatcaat
//
LOCUS       DATRUK010000084         5605 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.084, whole genome shotgun sequence.
ACCESSION   DATRUK010000084 DATRUK010000000
VERSION     DATRUK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.084"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..322
                     /locus_tag="VBE34_001796"
     CDS             <1..322
                     /locus_tag="VBE34_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO7264485.1"
                     /translation="MRVSFYHYLLMILLTLVAVTAMQSVGTILIVALLITPAATAYLY
                     VKSLRTMLFLSSALGAVASVLGLYIGYTFNIAAGSSIVLTSTFMFLLAFLFSPKQSLF
                     KKKQ"
     gene            complement(374..1177)
                     /locus_tag="VBE34_001797"
     CDS             complement(374..1177)
                     /locus_tag="VBE34_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO7264486.1"
                     /translation="MKKIIYLGLACVSILTLSGCESIERSLKGDRYVDQKLAENSSKE
                     ATEQLNKKTKQALKADKKAFPQLDKAVAKNEAQVLIKTSKGDINIKLFPKYAPLAVEN
                     FLTHAKEGYYNGLSFHRVIKDFMIQSGDPNGDGTGGKSIWNSKDKKKDSGNGFVNEIS
                     PYLYNIRGSLAMANAGADTNGSQFFINQSQQDHSKQLSDKKVPKVIIKAYSEGGNPSL
                     DGGYTVFGQVISGMETVDKIASVEVTKSDQPKEKITITSIKVIKDYKFK"
     gene            1343..3793
                     /locus_tag="VBE34_001798"
     CDS             1343..3793
                     /locus_tag="VBE34_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO7264487.1"
                     /translation="MVFMANKKKTKGKKTRRPTKAEIERQRAIQRMITALVLTIILFF
                     GIIRLGIFGITVYNVIRFMVGSLAYLFIAATLIYLYFFKWLRKKDSLVAGFLIASLGL
                     LIEWHAYLFSMPILKDKEILRSTARLIVSDLMQFKITVFAGGGMLGALIYKPIAFLFS
                     NIGAYMIGVLFIILGLFLMSSLEVYDIVEFIRAFKNKVAEKHEQNKKERFAKREMKKA
                     IAEQERIERQKAEEEAYLASVNVDPETGEILEDQAEDNLDDALPPEVSETSTPVFEPE
                     ILAYETSPQNDPLPVEPTIYLEDYDSPIPNMRENDEEMVYDLDDDVDDSDIENVDFTP
                     KTTLVYKLPTIDLFAPDKPKNQSKEKDLVRKNIRVLEETFRSFGIDVKVERAEIGPSV
                     TKYEIKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSF
                     RELWEQSDANPENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISS
                     ILMKARPDQVKFMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYE
                     LFSKIGVRNIAGYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIR
                     LGQKARAAGIHMILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKL
                     LGRGDMLFKPIDENHPVRLQGSFISDDDVERIVGFIKDQAEADYDDAFDPGEVSETDN
                     GSGGGGGVPESDPLFEEAKGLVLETQKASASMIQRRLSVGFNRATRLMEELEAAGVIG
                     PAEGTKPRKVLMTPTPSE"
     gene            3875..5590
                     /gene="pabB"
                     /locus_tag="VBE34_001799"
     CDS             3875..5590
                     /gene="pabB"
                     /locus_tag="VBE34_001799"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEO7264488.1"
                     /translation="MHIETVIDFKELGKRYRFKNPTKELIADTLEQVLEVIKEVDYYQ
                     SQNYYVVGYLSYEASAAFDSHFKVSQQKLAGEHLAYFTVHKDCENEAFPLSYENVRLA
                     DNWTANVSEQEYQEAIANIKGQIRQGNTYQVNYTLELSQQLCSDPFSVYERLMVEQGA
                     GYNAYIAYDDKRILSVSPELFFKKKDEVLTTRPMKGTSARKPTYQEDVAERDWLANDP
                     KNRSENMMIVDLLRNDMGRICDVGTVKVKKLCQVEQYATVWQMTSTIEGVLSPEVTLM
                     SIFQALYPCGSITGAPKISTMAIINELEKRPRGIYCGTIGLCMPDGQAIFNVPIRTVQ
                     MKGQQAYYGVGGGITWESQTDSEYEETRQKSAVLTRVNPKFQLITTGRVTENKLLFSQ
                     QHVERLVESASYFAYSFDKSKFERELKKYLHQLDEKDYRLKIMLDKTGKVTFEVKQLV
                     NLSKKFLTAEVVVQDYPIKLSPFTYFKTSYRPHIIEGQNEKIFVSPEGLLLETSIGNI
                     VLEKNGRFLTPDLSEGGLNGIYRRHLLKNQKVIEAPLTLKDLESADAIYACNAVRGLY
                     PLNLK"
BASE COUNT         1811 a          866 c         1114 g         1814 t
ORIGIN      
        1 aatgagagtt tctttttatc attatttatt gatgattttg ttaacattag ttgcagtaac
       61 agctatgcag agtgtgggaa caatattgat cgtagcactt ttaattacac cagctgcaac
      121 agcatatctt tatgttaaaa gtctcagaac aatgttgttt ttatcatctg cattaggagc
      181 agtagcatct gttctggggt tatatatcgg ttatacattt aatattgccg caggatcaag
      241 tattgtttta acatctactt ttatgttttt acttgctttc ctattttctc ctaaacaaag
      301 tttgtttaaa aagaaacaat agtgttgtta aaaaatgaga ctagaaaatg ttctagtctc
      361 atttttgata gaattattta aatttgtagt ctttaataac tttgatactt gtaatagtaa
      421 ttttttcttt tggttgatct gattttgtaa cttctaccga agcaatttta tccacagttt
      481 ccatgccaga gattacttgt ccaaagacgg tataaccacc atctaaactt ggatttcctc
      541 cctctgaata ggctttaata atcaccttag gaactttttt atctgacagt tgtttggaat
      601 gatcttgctg gctttgattg ataaagaatt ggctgccatt agtatcagct ccagcatttg
      661 ccattgctaa acttcccctg atattgtaga ggtaagggga tatttcatta acaaaaccat
      721 ttccagaatc tttcttttta tctttgctat tccatataga ttttccaccg gtcccgtctc
      781 cattagggtc accagattgt atcataaaat ctttgataac tctgtggaaa cttaacccat
      841 tatagtatcc ttcttttgca tgtgttagaa aattttcaac agctaaagga gcatattttg
      901 gaaataactt gatatttata tcacctttag aggtttttat aagcacttgt gcctcgtttt
      961 tggcaacagc cttgtccaat tgaggaaaag cttttttatc agcttttaag gcttgcttag
     1021 tttttttgtt tagttgttca gtagcttcct ttgaagagtt ttcagctagt ttttggtcaa
     1081 cataacggtc tccttttaga gaacgctcaa ttgactcgca accacttaaa gttaatatac
     1141 tgacacaggc taaaccaaga taaataattt ttttcataca tattccttga ttaagttatt
     1201 aagatgatta tattttatca taaattttta aataatttat aaaagtaaat agtaaaccta
     1261 ttgggggact tgttgaaatt atgatagaat agtatagcat gagttgatag agaaagtgag
     1321 gaacaagtat ttcacttgaa atatggtatt tatggcaaat aagaaaaaaa caaaaggaaa
     1381 gaaaaccaga agacctacta aggcagaaat agagcgtcaa agagctattc aaaggatgat
     1441 tactgctctt gttttaacaa ttattctctt ctttggtatt atcagattag gtatttttgg
     1501 tattacagtc tataacgtca tccgttttat ggtaggtagc ttggcttact tatttattgc
     1561 ggcaacttta atctaccttt atttctttaa atggttgcga aagaaagata gcttagtagc
     1621 aggttttttg atagcttctt taggattatt gattgagtgg catgcttacc ttttctcaat
     1681 gcctattttg aaagataaag aaattttgcg ttcaactgct cgattaattg tgtctgattt
     1741 aatgcaattt aaaatcactg tttttgccgg tggaggtatg ttgggtgctt tgatttacaa
     1801 gccaattgct tttctctttt ctaatattgg tgcctatatg attggtgttc tcttcatcat
     1861 tttgggtctc tttttaatga gttctctgga agtttatgac atcgtcgaat ttattagagc
     1921 ttttaaaaat aaagtggcag agaagcacga gcaaaataaa aaggagcgtt ttgctaagcg
     1981 agagatgaaa aaagcaatcg ctgaacaaga gcgcatagag cgtcaaaaag ctgaagaaga
     2041 agcttattta gcttcggtta atgtagaccc tgaaacgggt gagattctag aggatcaagc
     2101 tgaggacaat ttggatgatg cgctaccacc tgaggtaagt gaaacatcaa ctccggtatt
     2161 tgagccagag atccttgctt atgagacatc gcctcaaaat gatcctttac cagtagagcc
     2221 gacaatttat ttagaagact atgattcgcc gattcctaat atgagagaaa atgatgagga
     2281 aatggtttat gatttagatg atgatgtaga tgatagtgat atagaaaatg tcgactttac
     2341 acctaaaacg acactggttt ataaattacc aacgatagat ttatttgcac cagataagcc
     2401 taaaaatcaa tccaaagaaa aggatttagt ccgaaagaat atcagagttt tagaagaaac
     2461 atttagaagt tttggtatcg atgtaaaagt agaacgtgct gaaattggac catcagttac
     2521 taaatatgaa attaaaccag cagttggagt tcgtgtgaat cgtatttcaa atctatctga
     2581 cgacctagct cttgctcttg cagcaaaaga tgtgcgtata gaagcaccaa ttcctggaaa
     2641 atcattaata ggtattgaag ttcctaactc agaaattgca acggtttctt tccgcgaact
     2701 ttgggaacaa tctgatgcca atcctgaaaa ccttttagaa gtaccactag gaaaagctgt
     2761 taacggcaat gctcgcagtt ttaacttagc tagaatgccg catcttttgg tagctggttc
     2821 aactggttca ggtaaatctg tggcagttaa tggaattatt tcaagtattt tgatgaaggc
     2881 acgtccagat caagttaagt ttatgatgat tgatcccaaa atggttgaat tatctgttta
     2941 taatgatatt ccacatttat taatccctgt tgtaaccaat ccgcgtaaag caagtaaggc
     3001 actccaaaaa gttgttgatg aaatggaaaa tcgatacgag ttatttagca aaattggtgt
     3061 gcgtaatata gcaggttata atacaaaggt tgaagagttt aatgcttcct ctgagcaaaa
     3121 acaaatacct ttgcctttaa tcgttgtcat tgtagatgaa ttggctgact tgatgatggt
     3181 tgctagtaaa gaagttgaag atgctattat tcgtttgggg caaaaagcac gtgctgcagg
     3241 tatccatatg attcttgcaa ctcaacgtcc atccgtagat gttatttctg gtttgattaa
     3301 agcaaatgtt ccgtcgcgta ttgcatttgc tgtttcaagt ggtactgata gccgtacgat
     3361 ccttgatgaa aatggtgctg aaaagctctt gggacggggt gacatgctct ttaagcctat
     3421 tgatgagaat catccagtac gactacaagg ttcctttatt tcagatgatg atgttgaaag
     3481 gatcgttggt tttatcaaag accaagccga ggctgactat gatgatgcct ttgatcctgg
     3541 agaagtatct gaaacagata acggctctgg tggtggcggc ggagtacctg aaagtgatcc
     3601 tctttttgaa gaagccaagg gactcgtttt agagacgcaa aaagcaagtg cctcaatgat
     3661 tcaacgccga ttgtctgttg gtttcaatag agcaacaaga ctaatggaag aattagaagc
     3721 agcgggggtt attggtccag cagaaggaac caagccacga aaagttttaa tgactccaac
     3781 tccgagtgaa taagaaaaaa ctaatctgac aagaaattgt cggattagtt ttttatgtta
     3841 taatgaagca aatagatgaa gaagaaggtt acgaatgcat attgagactg ttattgattt
     3901 caaagaatta ggaaaaagat atcgttttaa aaatcctaca aaagaattaa tagctgatac
     3961 tttagaacaa gtcttagaag tgataaaaga agttgattat tatcaatctc aaaattatta
     4021 tgttgttggt tatttatctt atgaagcatc tgctgctttt gattcacatt ttaaagtttc
     4081 tcaacagaag ttggctggag aacatctagc ttattttaca gtacataaag attgtgagaa
     4141 cgaagctttt cctttaagtt atgaaaatgt tagattagca gataattgga ctgctaatgt
     4201 ttctgagcaa gaatatcaag aggcaattgc taatattaaa ggacaaatta gacaaggaaa
     4261 tacttatcaa gtaaattata cactagagct tagccaacaa ttatgctcgg atccctttag
     4321 tgtttatgaa cgcctaatgg tagaacaagg agcaggatat aatgcttata ttgcctacga
     4381 cgataaaagg attttatctg taagcccgga attgttcttt aagaagaagg atgaagtctt
     4441 aacaactaga ccaatgaagg gaacaagcgc tagaaagcct acttatcaag aagatgtggc
     4501 ggagcgagat tggttggcaa atgatcctaa aaatcgttct gaaaatatga tgattgttga
     4561 tttattaaga aacgatatgg gccgtatctg tgatgttgga acagttaagg taaaaaaact
     4621 atgtcaagtg gagcagtatg caactgtgtg gcaaatgaca tcaactattg aaggagtttt
     4681 atcgccagaa gtgacactta tgtctatttt tcaagctcta tatccttgtg gatctatcac
     4741 tggagctcct aagatatcaa caatggctat tattaatgag ttggaaaaac ggccaagagg
     4801 tatttattgt gggacgatag gactttgcat gccagacgga caagctattt ttaacgttcc
     4861 tattcgcaca gtacaaatga aaggtcaaca agcctattac ggcgttggtg gaggtattac
     4921 gtgggagagc cagacagatt ctgaatatga agaaacccgt caaaaatcag ctgttttaac
     4981 acgtgtcaat ccaaaatttc aattaataac tacaggaaga gtgactgaaa ataaacttct
     5041 tttctctcaa caacatgtcg agagattagt tgaatcagcg tcttattttg cttattcttt
     5101 tgataaaagt aaattcgaaa gggaactgaa aaaatacctt catcagctag atgagaagga
     5161 ttaccgcttg aaaataatgc ttgataaaac tggtaaggta acgtttgagg taaaacaact
     5221 agtgaattta tcaaaaaagt ttttaacggc agaggtggtc gtacaagact accctattaa
     5281 attatccccg tttacttatt ttaaaacttc ttatcgccca catattattg aaggtcagaa
     5341 tgaaaagata tttgtatctc ctgaggggtt gctattggaa acaagtattg ggaatattgt
     5401 tttagaaaaa aatggaaggt ttttaacccc agatttatca gaaggagggt tgaatgggat
     5461 ttatcgtcgt catctcctta aaaaccaaaa agtaattgaa gcaccactaa ctttaaaaga
     5521 tttagaatca gccgatgcta tatacgcctg taatgctgtt agagggcttt atcctctaaa
     5581 cctaaagtaa aaagagttgg aaatt
//
LOCUS       DATRUK010000085         5137 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.085, whole genome shotgun sequence.
ACCESSION   DATRUK010000085 DATRUK010000000
VERSION     DATRUK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.085"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..823
                     /locus_tag="VBE34_001800"
     CDS             <1..823
                     /locus_tag="VBE34_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO7264489.1"
                     /translation="VAFGFNAFFLHNHIVTGGISGLAVSFHEVFGWDSAFFIMIINIP
                     LLLLCYFGLGKQTFLKTVYGSWIFPVFIKLTQSVPTLTHNPLLAALFGGVIVGCGLGI
                     VFWSDSSTGGTGIIIQFLGKYTPISLGQGVILIDGLVTIVGFLAFDSDTVMFSIIGLI
                     TISYIINAIQTGFTTLSTVLIVSQEHQKIKTYINTVADRGVTEIPVKGGYSGTNQIML
                     MTTIAGYEFAKLQEAIAEIDETAFITVTPTSQASGRGFSLQKNHGRLDEDILMPM"
     gene            889..2508
                     /locus_tag="VBE34_001801"
     CDS             889..2508
                     /locus_tag="VBE34_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7264490.1"
                     /translation="MLTVSDVSLRFSDRKLFDEVNINFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHIALGPDERLSVLRQNHFDYEDERVIDVVIMGNETLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNISEELHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKESSELAARLQADRNAKAEEKIKQLQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFVNFKAEREMGNDLLTVENLSVTIDGEKI
                     LDNISFILRPGDKTALIGQNDIQTTALIRALMGDIEYEGTIKWGVTTSRSYLPKDNSR
                     DFASGESILEWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKDFKESIIFASHDHEFIQTLANHIIIL
                     SKNGVIDRIDETYDEFLENTEVQAKVAQLWK"
     gene            2631..>5137
                     /locus_tag="VBE34_001802"
     CDS             2631..>5137
                     /locus_tag="VBE34_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEO7264491.1"
                     /translation="MTINHYKLRIPYYTISFLLPFIIIVCILFTKNIYWGSPTTILAS
                     DSFHQYVIFNQALRNILHGSNSLFYTFTSGLGLNFYALSSYYLGSFLSPIVYFFNLKN
                     MPDAIYLLTICKVGLIGLSMFVTLCKRHCKVNRVLLLVISTCYSLMSFSISQIEINMW
                     LDVFILIPLVVLGVDQLLWERKPILHFLSLTALFIQNYYFGFMTTIFTSLYFIVQITR
                     NTDSKVAFKQFLHFTFLSLLAGMTSSIMILPTYFDLTTHGEKLTKVSKMFTENSWYMD
                     LFAKNMIGAYDTTKFGSIPMIYVGLLPLLLSLLYFTIKEIPRRTRLAYGFSLIFVIAS
                     FYITPLDLFWQGMHAPNMFLHRYSWVLSVLICLLAAECLEYLDNISWKKILGVNLILI
                     SGFIITFLFKKHYHYLNLELLLLTLTFLSAYIILTISFVSKQIPKLVFYPFLIGFVVL
                     EMTLNTFYQLNSLNDEWVFPSRQGYAKYNHSISKLVRKTERNNSTFFRTERWLGQTGN
                     DSMKYNYNGISQFSSIRNRSSSQVLDRLGFKSDGTNLNLRYQNNTLIADSLFGVKYNL
                     TEYPFDKFGFIRKAQDKQTILYENQFASQLAILTNQVYQDKPFTVNTLDNQTTLLNQL
                     SGLKETYFEHLIPNSVSGQTTLNKQVFVRKNKQGNTEITYNITIPKNSQLYVSMPFIN
                     FNNEENKIVQISVNNGPFVPNTLDNAYSFFNIGSFAENSRIKVKFQFPHNDQVSFPIP
                     HFYGLKLEAYQKAMTVINKRKVKVRTDHNKVIANYTSPNRSSLFFTIPYDRGWKAYQN
                     NKGIKIFKAQKGFMKINIPKGKGKVTLIFIPYGFKFGV"
BASE COUNT         1654 a          849 c          852 g         1782 t
ORIGIN      
        1 tgttgcattt ggtttcaacg ctttctttct tcataatcat attgtcacgg gggggattag
       61 tggtttggca gttagttttc atgaagtatt tggttgggat tctgcttttt ttattatgat
      121 tatcaatatt ccattgctcc ttctttgcta ctttggctta ggtaaacaaa cctttttaaa
      181 aactgtctat ggttcttgga tttttcctgt ttttattaag ttaacacaaa gtgtaccaac
      241 tttgacccac aacccactcc tcgcagcact ttttggaggt gttattgtag gatgtggttt
      301 ggggattgtt ttttggagcg actcttcaac tggtggaacg gggattatca ttcaattctt
      361 aggaaaatat actcctataa gccttggaca aggggttata ttgattgatg gacttgttac
      421 aattgttggt ttcctagctt ttgacagtga tacggttatg ttttctatta ttgggttgat
      481 aactattagt tatattatta atgctatcca aactggattt acaaccttaa gcactgtctt
      541 aatcgtttct caagagcacc aaaaaattaa gacatatatc aatactgtcg cagatagagg
      601 agtaacagaa attcccgtta aagggggata ttctggaact aatcaaatca tgcttatgac
      661 aactattgct ggttatgagt ttgctaaatt acaagaggca atagcagaaa ttgacgaaac
      721 agccttcata acagtaactc caacatcaca agcttctgga cgtggattta gtcttcaaaa
      781 aaatcatgga cgtcttgatg aagacattct tatgccaatg taatcgattg ataattaaag
      841 ctttttctag tagaatagca gatagaatat acacaagagg taacaaaatt gcttacagtt
      901 tcagatgttt cactacgttt tagtgatcgc aaattatttg atgaagttaa cattaacttt
      961 acagcaggaa atacctatgg tcttatcgga gcaaatggcg ctggtaaatc aactttcctt
     1021 aaaattttag ctggtgatat tgaaccaaca acgggacata ttgcacttgg cccagatgaa
     1081 cgcctttctg tacttcgcca aaatcatttt gattatgaag atgagcgcgt cattgatgtc
     1141 gttatcatgg gtaatgaaac actttatagt atcatgaaag aaaaggatgc tatctacatg
     1201 aaagaagatt tttctgatga agatggtgtt cgcgctgcgg aattagaagg agaatttgct
     1261 gaactcggtg gatgggaggc tgaaagtgag gcttctcaat tactccaaaa tctaaatatt
     1321 tcagaagagc ttcattatca aaatatgagt gaattagcta atggagacaa agttaaagtt
     1381 ctccttgcaa aagctctctt tggtaaacca gatgtacttc tccttgacga gcctaccaat
     1441 ggacttgata tccagtctat tacttggtta gaagatttct tgattgactt tgaaaataca
     1501 gttattgtag tttcccacga ccgtcatttc ttgaataaag tatgtacaca tatggctgac
     1561 cttgattttg gaaaaattaa actgttcgtc ggcaactatg atttctggaa agaatcatca
     1621 gaattagcag ctcgtttaca agctgatcgt aatgctaaag cagaagaaaa aattaaacaa
     1681 cttcaagaat tcgttgcacg attttcagcg aatgcttcaa aatcaaaaca agcaacttct
     1741 cgtaaaaaaa tgcttgataa aattgaactt gaagagattg taccatcaag ccgtaaatac
     1801 ccattcgtta actttaaagc cgaacgcgaa atgggaaatg atttattgac cgttgaaaat
     1861 ctctctgtca ctattgatgg ggaaaaaatc cttgataata tcagtttcat tcttcgtcct
     1921 ggtgataaaa ctgcacttat tggacaaaat gatatccaaa ctactgcact tattcgagca
     1981 ttgatggggg atatcgaata tgaaggaaca atcaaatggg gagtaacaac tagtcgttct
     2041 taccttccaa aagacaattc acgagacttt gcttctggcg agtcaatctt agaatggctt
     2101 cgtcaatttg ctagtaaaga agaagacgat aataccttct tacgtggctt cctaggtcgt
     2161 atgcttttct ctggagatga ggttaacaaa tctgttaatg tcttatctgg gggtgaaaaa
     2221 gtacgcgtta tgctttcgaa attaatgcta ttaaaatcta atgttcttgt tcttgatgat
     2281 ccaacaaacc acttagattt ggaatctatt tcaagtctca atgatggact aaaagatttc
     2341 aaagaatcta ttatttttgc aagtcatgac cacgagttta ttcaaacttt agcgaaccat
     2401 attattattc tttctaaaaa cggagttatc gatcgtatcg atgaaactta cgatgaattt
     2461 cttgaaaata ccgaagtaca agctaaggtc gctcaactct ggaaataatc ctttggcata
     2521 tctctaacct aataaacgtg tcaagattga aaatcttaaa actatattaa tcatttaaat
     2581 aaaaaggttg gaacaaatag cgtccagcct ttttatctta ggagatatat atgacaatta
     2641 accactataa gcttcgtata ccttactata caataagctt tctacttccc ttcataatca
     2701 tagtctgtat cctctttact aagaatattt attggggaag tccaacaacc atattagcaa
     2761 gcgatagctt tcatcaatat gttatcttta atcaagcatt aagaaatatt cttcatggtt
     2821 caaacagctt attctatacc tttacaagcg gtttaggttt aaatttttat gctctgagta
     2881 gttactattt gggaagtttt ttatccccaa tagtttattt ctttaaccta aaaaatatgc
     2941 ctgatgctat ctacctttta acaatatgta aagtagggtt gataggtttg tcaatgtttg
     3001 tcactctttg taaacgtcat tgtaaagtta atcgtgtctt acttttagta atttctactt
     3061 gctatagctt aatgagtttc tcaattagcc aaattgaaat taatatgtgg ctagatgtat
     3121 ttattctaat tccccttgtt gttttaggag ttgatcagct actatgggaa agaaaaccta
     3181 tacttcattt tctctctctg acagccttat tcatccaaaa ctattatttt ggttttatga
     3241 ctaccatctt tactagcctt tacttcatcg tacaaataac ccgtaatact gatagtaagg
     3301 ttgcatttaa acaatttctt cattttacct ttttatctct tttagctggt atgactagta
     3361 gcattatgat tttgccaact tattttgatt taactactca tggtgaaaag ttgacaaagg
     3421 tatcgaagat gtttacagaa aattcttggt acatggattt gtttgctaaa aatatgattg
     3481 gagcctatga tacaacaaaa tttggctcta ttcctatgat ttatgtaggt ttacttcctt
     3541 tactcctttc tttactttac tttacaataa aggaaattcc tcgaaggact agattagcat
     3601 atggattttc actaatattt gttattgcta gtttttatat aacacctcta gatttatttt
     3661 ggcaagggat gcatgctcca aatatgtttc ttcatcgcta ctcttgggta ctttcagtac
     3721 ttatctgtct actagctgct gaatgccttg agtatttaga caacatttct tggaaaaaga
     3781 ttttaggggt caatttaatt ttaataagtg gtttcataat cacgttttta tttaaaaagc
     3841 actatcatta ccttaatctt gaattacttc ttttaacctt aacattcctc tcagcttata
     3901 ttattctaac tattagtttt gtcagtaagc aaatccctaa attagttttt tatccctttc
     3961 ttattggttt tgttgtgtta gagatgacat taaacacctt ttaccaacta aatagtctta
     4021 acgatgagtg ggtatttcca tctaggcaag gatatgctaa atataatcac agcattagca
     4081 aacttgtcag aaagactgag agaaataact ctacattctt ccgtacagag cgctggttag
     4141 gacaaaccgg caacgactct atgaaatata attataatgg tatttcacaa ttttcatcta
     4201 ttagaaatcg ttcttcaagt caagtgcttg atagacttgg ctttaaatca gatggtacaa
     4261 atttaaattt acgttatcaa aataatactt tgatcgcaga tagtctattt ggtgtaaagt
     4321 acaaccttac agaatatcct tttgataaat ttggttttat cagaaaagct caagataaac
     4381 aaaccattct ttacgaaaat cagtttgcca gtcaattagc tatactaacg aatcaagtat
     4441 atcaagataa accatttact gtgaatactt tggataatca gacaactctt ttaaatcaat
     4501 taagtggttt gaaagagacg tattttgagc atcttattcc aaatagcgtt tctgggcaaa
     4561 ctactcttaa taaacaagta tttgttagaa agaataaaca agggaatact gaaattacct
     4621 ataacataac tatccctaaa aacagtcagt tatatgttag tatgccgttt ataaatttta
     4681 ataatgagga aaataaaatt gttcaaatat ccgttaataa tggaccattt gtacctaata
     4741 ctcttgataa tgcttattca ttttttaata ttggttcctt tgctgaaaac agtcgtatca
     4801 aagtaaaatt tcaatttcca cacaatgatc aagtttcttt cccaattccc cacttttatg
     4861 ggctcaagtt agaagcttat caaaaagcta tgactgttat taataaacga aaagtaaaag
     4921 taagaactga tcacaataaa gttattgcta attatacaag tcctaataga agttctcttt
     4981 tcttcactat tccctatgat agaggttgga aagcttatca aaataataaa ggaataaaaa
     5041 ttttcaaagc tcagaaaggt tttatgaaaa taaatattcc taaaggaaaa ggcaaagtaa
     5101 cattaatttt tatcccttac ggctttaaat ttggggt
//
LOCUS       DATRUK010000086         5061 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.086, whole genome shotgun sequence.
ACCESSION   DATRUK010000086 DATRUK010000000
VERSION     DATRUK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.086"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            228..653
                     /locus_tag="VBE34_001803"
     CDS             228..653
                     /locus_tag="VBE34_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264492.1"
                     /translation="MKKINLNSTKLGELRFSVNEIEAPQVAIVTNARRVAKRDGNGEV
                     IDGTIAKLTILVLDIKLYQLMNQQGLDVSSVVPITVEVVGDENYLKQIIVEDIIGKVI
                     DLRSAYVGLKWVSRGNSGNWGGLKLILEELKFVNLNKDN"
     gene            728..2134
                     /locus_tag="VBE34_001804"
     CDS             728..2134
                     /locus_tag="VBE34_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO7264493.1"
                     /translation="MMKTIFKEIISTYSLKNKKVATIIVSRTRKQRCQLLTNILLSIL
                     SVEMMFGLLYLLFDFVNSNQISISIIFSLVGLVCLESIMVLNWLFLNSKFSPIEKWKV
                     SALLKLFPEENDILRKHKSEIDTTQSVRWEYWIDNGLIFLKLYHGGHVPKGKLEEIPT
                     NILSFFIKETRDEWFLEDKNLADGYVKVVLSHKPDKSLYIKGREDFIESKTLKIRLTE
                     RLNWTLSQTNALIVGPSNSGKTTLLKYLILAFLINNKKNEVYTIDGKSAYLSNAMRHI
                     GEVATTGQQAFDMISKVEAIMEERYAEINQNSDDEADVTHNEKFGQGNILLVIDEYLA
                     LATEMQAEDKLKKPADRLFPQFNAKLLKLIVKCRQASIMVVVSGQLLPTSILSSESRD
                     SLGLRIALGRISQSQSTEIFNIGKSELPSVDSSKREGVIYLDGIGLEHPITFKTPFYD
                     DSRLPFKGALRVLKSERS"
     gene            2236..3105
                     /locus_tag="VBE34_001805"
     CDS             2236..3105
                     /locus_tag="VBE34_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264494.1"
                     /translation="MTSEYNLKVINFGDHVEVIKYGYLKQRVSGNNRPTNHERQIRTE
                     EEQEIYNFQQAQKIQRRIRYYCECNDFDLFWTLTFDDEKVNAKDYEYAKKKLRAWLKY
                     QREKYGKFDYIFIPELHKNGRIHFHGITFGFSPPLIEARNPKTDKLIKKNGLQIYNAE
                     TWKDGFSTVSKIDSKAKTANYISKYITKDLIAIPSAYRQPKYFVSRGLKKPEIEFQQI
                     DENELQEFTPNFIVGNLYIEEQKFEKDVSIYLLELDDENKFFQKKKPETVVRSRLKSK
                     NDENIENVSDENE"
     gene            3125..3382
                     /locus_tag="VBE34_001806"
     CDS             3125..3382
                     /locus_tag="VBE34_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEO7264495.1"
                     /translation="MTNTKVTVKHIDLMKIGFPENTAREIIKQAKAIAVQRFENNIQE
                     TGKVIQLGQSPFDNVRLNLAPASIVEELLGFELPLLEKEGA"
     gene            3385..4617
                     /locus_tag="VBE34_001807"
     CDS             3385..4617
                     /locus_tag="VBE34_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7264496.1"
                     /translation="MASKKYSGVSRDKKNRIYFQTEFGKDPVTGKRRRKKSYKDKNGN
                     PFRTEKQAYDELCRVRAEVRSQDELAQKQTVPVLTLEDFINTVFLPYYIKTVESSTYK
                     TALTHFDIFKEELGQYLLSDISVQFCERFRLSLQDKYSPNYAKNVWLRFKKCLTYAER
                     LEYIEFNPCTKLDNIKGERTVTSFWTVEDFQKAVNAFDRTTYQGVYYYTVIWLYFMTG
                     LRVSEGLSLIWSDIDFESKLLHVQSTLEYDENGNPFRKNKTKTEAGTRYVELDDMTLQ
                     TLKEWKEVQIHNSLGDFVLAREEVPMHKTTLTRLLKRVAKEQDLPVMAGKGLRHSHDS
                     FLINVLHKDVLYVSARSGRTDKATTLNTYAHIYAKKKAEGGREITKALESFGFDNPTK
                     TPPSKEEIAETLEITRFN"
     gene            complement(4778..>5061)
                     /locus_tag="VBE34_001808"
     CDS             complement(4778..>5061)
                     /locus_tag="VBE34_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264497.1"
                     /translation="GLGEADYSYFQSIFDSINLYSSDTVLIFYWSCYNENIREQIKSQ
                     QVHAVHKLIEKYGDTFNNKNHGRNLFTKLQLENRLIIQEILYNDILNPY"
BASE COUNT         1895 a          688 c          947 g         1531 t
ORIGIN      
        1 tggcagtcag tcccaaccaa atattggggg cgaaaatagt aaactaaaag tttgaaaact
       61 ccccctacca aagttctctg ttctctatat attaaggtta atattaggta gctagttctc
      121 aaattaaagt aacagaggaa cgaccataga attttatagc tataaagtct aatcaatgct
      181 tgtcggctta gttaagacat aaataattta tatagaggta aataacattg aaaaaaatca
      241 atttaaatag tacaaaactt ggagaacttc gtttttcagt aaatgaaatt gaagctcctc
      301 aagtagcaat agtcaccaat gctagaagag tagcaaaaag agacggcaat ggtgaagtta
      361 ttgatggaac aatcgcaaag cttacgattt tagttttaga tattaagcta tatcaactta
      421 tgaatcaaca aggattagat gtttcaagtg ttgtacctat tacagtagaa gtcgtaggtg
      481 atgaaaatta tctaaaacag attattgttg aagatataat tggaaaagtt attgatttac
      541 gttcagccta tgtaggtttg aaatgggttt ctagaggcaa ttcaggaaac tggggtggat
      601 taaaattaat tttagaagaa ttgaaatttg ttaatttaaa taaagataac taaaaattaa
      661 ataggaatat tagggatagg gggtagcaat gctacatggg tatatatttt taacaaggaa
      721 aaaattaatg atgaaaacaa tatttaaaga aataataagt acttactcac taaaaaataa
      781 gaaagtagca actatcatag tgagcaggac acgaaaacaa cgctgtcagc tattgactaa
      841 tattttgttg tctatcttat cagttgagat gatgtttggc ttactatacc tgctctttga
      901 ttttgtgaac tctaaccaaa tttcaatatc aatcatattt agcttagtag gcttagtttg
      961 cttggaaagt attatggttc tgaactggtt attcttaaac tctaagtttt ctcctattga
     1021 aaaatggaag gtgtcagcct tattaaagct ctttcctgaa gaaaatgata ttttgagaaa
     1081 acataaatca gaaattgata caacacaaag cgttagatgg gaatattgga tagataatgg
     1141 cttgatattt ttgaaattat atcacggtgg tcatgttcca aaaggcaaat tggaggaaat
     1201 cccaactaat attctatcat ttttcattaa agaaacacgt gatgaatggt ttttagaaga
     1261 taagaaccta gcagatggat atgtgaaggt tgtcttgtct cacaaaccag ataaatcact
     1321 ttatataaaa gggcgtgaag attttataga atcaaagaca ttaaagattc gattgactga
     1381 aagactgaat tggacattat cacaaactaa tgctcttatt gttggaccgt caaactctgg
     1441 taaaacaacc ttactgaaat atctaattct agcattcttg ataaataata agaaaaatga
     1501 agtttatacg attgatggta aatcagccta tctaagtaat gcgatgcgac acattggtga
     1561 agtagcaact acagggcaac aggcgtttga tatgatttct aaagttgaag ctattatgga
     1621 agaacgttat gctgaaatca atcaaaattc tgatgatgaa gcagatgtga cacataatga
     1681 aaaatttggg caaggaaata ttttgctagt tattgatgag tatttagctc tagctacaga
     1741 aatgcaagca gaggataaat taaagaaacc tgctgataga ctgtttccac aatttaatgc
     1801 caaacttttg aaattaattg ttaaatgtag acaagcttct attatggttg ttgtttcggg
     1861 tcagctgttg cctacgtcta ttttgagctc tgagagtaga gattcacttg gtttacgtat
     1921 tgctttgggt cgtattagtc aatctcaatc aacagaaatt tttaatatag gaaaatcaga
     1981 attgcccagt gtggactcaa gtaaacgaga aggtgttatc tacttagatg gtattggatt
     2041 agagcaccca attactttca agacaccatt ttatgatgat tctaggctac cattcaaggg
     2101 ggcgttacgt gttctgaaat cagagcggag ctagctccgc tctgattcta ggaataaata
     2161 aggatacttt tggctggcta cttgacagac agccaagata agctgagggt atgatagaaa
     2221 gttggtaaat tatgagtgac tagtgaatac aatttaaaag ttataaattt tggagaccat
     2281 gttgaggtaa taaaatatgg ttatctaaaa caaagagtat caggtaataa tagacctacc
     2341 aaccatgaaa gacaaatacg tacagaagag gaacaagaga tatataattt tcagcaagct
     2401 caaaaaattc aacgaagaat tagatattac tgtgagtgta acgattttga tttattctgg
     2461 acattaactt ttgatgatga aaaagttaat gcaaaagatt atgaatatgc gaaaaagaaa
     2521 ttaagagctt ggttgaaata tcagcgtgaa aaatatggaa aatttgacta tatttttata
     2581 ccagaattac ataagaatgg tagaatccat tttcacggaa taacttttgg attctcacca
     2641 ccattaatag aagcaaggaa cccaaaaacg gataaattga taaaaaagaa tggtttgcaa
     2701 atatataatg ctgaaacttg gaaagatggt ttcagtaccg tatcaaaaat tgatagcaag
     2761 gcaaaaacag caaattatat atcgaagtat attacaaaag atttgattgc tattccttcg
     2821 gcgtatagac agccaaaata ttttgttagt cgtggtttga aaaaacctga aatagaattt
     2881 cagcagattg atgaaaatga acttcaagag tttaccccta atttcatagt aggtaactta
     2941 tatattgaag aacaaaaatt tgaaaaagat gtctcaattt acctgttaga attagatgat
     3001 gagaataaat tttttcaaaa gaaaaaacct gaaacagtcg taagaagtag attgaaaagt
     3061 aagaatgatg agaatattga gaatgtttca gatgagaatg aataagaaaa ttaggattat
     3121 taatatgaca aatacaaaag taacagtaaa acatattgac ttaatgaaaa tcggattccc
     3181 agagaataca gctagagaaa ttataaagca agcaaaagcg attgcagtac agagatttga
     3241 aaataatata caggaaacag gaaaagtgat acaattagga caatcaccat ttgataatgt
     3301 gagattaaac ctagccccag ctagtatagt tgaggaattg cttggctttg agttgccttt
     3361 actagaaaag gaaggtgctt aaagatggca agtaaaaaat atagcggagt atccagagat
     3421 aagaaaaata gaatttactt tcaaacagaa tttgggaaag accctgtaac gggaaaacga
     3481 agacgtaaga agtcctataa ggataaaaat ggaaatccat tccgtactga aaaacaggct
     3541 tatgatgaac tttgtcgagt tagagccgaa gtcaggtcac aagatgaact tgcacaaaaa
     3601 cagacagttc cagtattaac gttagaagat tttattaaca cggtattttt accgtattat
     3661 atcaaaactg ttgaatcgtc tacctataaa acagcattga cacactttga tatttttaaa
     3721 gaagaactag ggcagtactt actttcagat atatctgttc agttttgtga acggttccgt
     3781 ctttcattac aagataagta ttcaccaaac tatgcaaaaa atgtttggct tagatttaaa
     3841 aaatgcttaa cttacgcaga gcgtttggaa tatattgaat ttaacccttg tactaaacta
     3901 gacaatatca aaggtgagag aacagtgact tcattttgga cggtagaaga ttttcagaaa
     3961 gcagtcaatg catttgatag aacaacatat caaggtgttt attactatac agtaatctgg
     4021 ctatatttta tgacaggatt acgagtttct gaaggattga gcctaatttg gtctgatatt
     4081 gattttgaat ctaaattatt gcacgtgcaa tcaactttag aatatgatga aaatgggaat
     4141 ccatttcgta aaaacaagac taagaccgaa gcaggtacga gatatgttga attagatgat
     4201 atgacacttc aaacgctaaa agagtggaaa gaagtacaga ttcataatag tttaggtgat
     4261 tttgttttag ctcgtgaaga agttcctatg cataaaacta ccctaactcg tcttttgaaa
     4321 cgtgtagcaa aagagcaaga tttaccagtt atggctggta aaggtcttag acattcgcat
     4381 gatagctttt tgattaatgt tcttcacaaa gatgttttat atgtatcagc ccgttctgga
     4441 agaacagata aagctacaac actcaataca tatgcccata tttacgctaa gaaaaaagct
     4501 gaaggtggta gagaaataac gaaggcttta gaatcgtttg gatttgataa ccccaccaag
     4561 accccaccaa gcaaagaaga gatagcggaa acacttgaaa taacaaggtt taattgaaaa
     4621 acgcaactgt tactcttaaa taatcatcta ttggttgttt aaaggtgaaa gcctagtttt
     4681 aaagtctttt cgttaaagtt aaggcgatta aaaaaggaaa gattcaattg aatcttcccc
     4741 acaaacaccc ccacaatatt ttgtgggggt gttttgttta atatggattt agaatatcat
     4801 tataaagtat ttcttgaata attaaacgat tttctaattg aagttttgtg aataaatttc
     4861 tgccatgatt cttattatta aaagtatctc catatttttc aattaattta tgtactgcat
     4921 gaacttgttg agatttaatt tgttccctaa tattctcgtt ataacaactc caatagaaaa
     4981 ttagtacagt gtcactggaa taaagattaa ttgagtcaaa aattgattga aaataagaat
     5041 agtctgcttc acccagacca t
//
LOCUS       DATRUK010000087         5010 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.087, whole genome shotgun sequence.
ACCESSION   DATRUK010000087 DATRUK010000000
VERSION     DATRUK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.087"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            124..1908
                     /locus_tag="VBE34_001809"
     CDS             124..1908
                     /locus_tag="VBE34_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEO7264498.1"
                     /translation="MNNNEKKVKYFLRKTAYGLASMSAAFIVCSGIVNTPTVSADSPD
                     TLKVEKLGKLKDVKSVHELTPISIPNELKGAKEQALSSIISHPNITNSEVDKLASDYS
                     FRINTSNDVNDVKRLLNEFYNAVARKQLDTNSADYRSKIDNISTTGLAIALEAKEIYE
                     ANKSILPHRYKDSVGTYVNSFEERRSPGKFNIWNGQEGFNAAQKLLEDVKKLLLELQN
                     LTKNNKPNIQVPKQAPTEAAKPALSPEALTRLTTWYNQAKDLLKDDQVKDKYVDILSV
                     QKAVDQAYDHVEEGKFITTDQANQLANKLRDALQSLELKDKKVAKPVAKGTYDVKYVD
                     TEGKEVAKSRHFEGEEGAAFVTSAKEVAGYKLVRTEGAVSNVFTAGAQVRTYVYEKVA
                     KPVAKGTYDVKYVDTEGKEVAKSRHFEGEEGAAFVTSAKEVAGYKLVRTEGAVSNVFT
                     AGAQVRTYVYEKVKPEVKPDVKPEAKPEAKPEVKPDVKPEAKPEAKPEVKSDVKPEAK
                     PEAKPEAKPEVKPDVKPEAKPEAKPATKKSVNTSGNLVAKKAIENKKYSKKLPSTGEA
                     ASPLLAIVSLIVMLSAGLITIVLKHKKN"
     gene            2114..2653
                     /gene="nusG"
                     /locus_tag="VBE34_001810"
     CDS             2114..2653
                     /gene="nusG"
                     /locus_tag="VBE34_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEO7264499.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGKTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRSILISMGQTVDVFDTNIKEGDVVQIIDGAFIGQEGRVVEIENNKVKLMI
                     NMFGSETQAELELYQVAEL"
     gene            complement(2700..3905)
                     /locus_tag="VBE34_001811"
     CDS             complement(2700..3905)
                     /locus_tag="VBE34_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 8 glycosyl transferase"
                     /protein_id="HEO7264500.1"
                     /translation="MEKAVALAVDSNYLDKALVTIKSICVYNRNITFYLFNQDTPVEW
                     VRNINRKLEPLGSKLINVKIYNYDIAHLTTFLTVSTWFRLFLADYIPSSRVLYLDSDI
                     IVNTNLDYLFELDFKGHYLAAVKDPHKNEEGGFNAGMLLANLELWREDGLTKTLLKTA
                     EELHRVVKTGDQSILNIVCHNRWLSLNKTWNFQTYDVVSRYNHRSYLYLNIENRTPNI
                     IHFLTSDKPWNENSVARFRELWWYYFQLDFCQLTGKQRKVISYEKSMELLSVSDIHLF
                     TLTSSDNLEHIESLICRCPTVQFHIGAYTTVSNKLSKLEQYPNVLVYPELIEARIEKL
                     ITLATAYLDINHGPEVGNILQRVHLKQKPIYSFNNTSHQENITKHIVNHNSIDNMVVL
                     INELNLNQL"
     gene            complement(3970..>5010)
                     /locus_tag="VBE34_001812"
     CDS             complement(3970..>5010)
                     /locus_tag="VBE34_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEO7264501.1"
                     /translation="RVGQTGNQIQDVKLFHDHLSPKWENKKLNHINYMTYARYFIPQY
                     ISADTVLYLDSDLVVTTNLDNLFQISLDNAYLAAVPALFGLGYGFNAGVMVINNQRWR
                     QENMTIKLIEKNQKEIENANEGDQTILNRMFENQVIYLDDTYNFQIGFDMGAAIDGHK
                     FIFDIPITPLPKIIHYISGIKPWQTLSNMRLREVWWHYNLLEWSSIISSKKVFGLDHP
                     IKTQNYRLNFLIVTTSDCIPSISELVTALPDCLFHIAAPTVMSDILISLLAFENIRLH
                     KTYTPPIIDKLLAEADIYLDINVFGKMEGILQKMSQSEKPILTTRECNSQLVPETVFA
                     SIDDLINYLQSI"
BASE COUNT         1789 a          771 c          917 g         1533 t
ORIGIN      
        1 aaaagtgttg aaataaaaaa ctccaagtta tataattagt taaggaactc aaattaaatt
       61 aaaataaacg tggtcctatc ctaataaatt aggattttta actaactata taagggagcg
      121 aatatgaata ataacgaaaa aaaagtaaaa tactttttaa gaaaaacagc ttatggtttg
      181 gcttcaatgt cagcggcgtt tatagtatgt agtggtattg taaatactcc tacagtgtct
      241 gctgatagtc ctgatacatt aaaagtcgaa aaattaggca aattgaaaga tgtgaaatca
      301 gttcatgaac tcacacccat atcaataccg aacgaattaa aaggtgctaa agagcaagca
      361 ctttcttcaa taatttcaca tcctaatata actaattcgg aagtagacaa actagctagt
      421 gactatagtt ttagaattaa tacatctaat gatgtgaacg acgttaaacg tctattaaat
      481 gaattttata acgcagttgc aaggaaacag ttagatacaa attctgctga ctaccgtagt
      541 aaaattgata atatcagtac tacaggtctt gcgatagctc ttgaggctaa agaaatttat
      601 gaagcaaata aatctatatt acctcatcgt tacaaagatt ctgttggaac ttatgtgaac
      661 agttttgagg aaagacgaag tccaggaaaa tttaatattt ggaatggtca ggaaggattt
      721 aatgcagctc aaaaattgtt agaagatgtt aaaaaattat tacttgagct acaaaattta
      781 acaaaaaata acaaaccaaa tattcaagta cctaaacaag cacctacaga agctgcaaaa
      841 ccagctttgt caccagaagc cttgacaaga ttgactacat ggtataatca agctaaagat
      901 ctgcttaaag atgatcaagt aaaggacaaa tacgtagata tactttcagt tcaaaaagct
      961 gttgaccaag cttatgatca tgtggaagag ggaaaattta ttaccactga tcaagcaaat
     1021 caattagcta acaagctacg tgatgcttta caaagtttag aattaaaaga taaaaaagta
     1081 gccaaaccag tagctaaagg tacatacgat gttaagtatg tagacacaga aggaaaagaa
     1141 gtagctaagt cacgtcactt cgaaggagaa gaaggcgcag cttttgtcac ttcagcgaaa
     1201 gaagtagcgg gttacaaact tgttagaacg gaaggcgctg tttcaaatgt cttcacagca
     1261 ggagcacaag tacgtacata tgtttacgaa aaagtagcca aaccagtagc taaaggtaca
     1321 tacgatgtta agtatgtaga cacagaagga aaagaagtag ctaagtcgcg tcacttcgaa
     1381 ggagaagaag gcgcagcttt tgtcacttca gcgaaagaag tagcgggtta caaacttgtt
     1441 agaacggaag gtgctgtttc aaatgtcttc acagcaggag cacaagtacg tacatatgtt
     1501 tacgaaaaag ttaaaccaga agttaaacca gacgttaagc cagaggccaa accagaggct
     1561 aagccagaag ttaaaccaga cgttaagcca gaggccaaac cagaggctaa gccagaagtt
     1621 aaatcagacg ttaagccaga ggctaagcca gaagccaaac cagaggctaa accagaagtt
     1681 aaaccagacg ttaagccaga ggctaaacca gaagccaagc cagcaaccaa aaaatcggtt
     1741 aatactagcg gaaacttggt ggctaaaaaa gctattgaaa acaaaaagta tagtaaaaaa
     1801 ttaccatcaa cgggtgaagc cgcaagtcca ctcttagcaa ttgtatcact aattgttatg
     1861 ttaagtgcag gtcttattac gatagtttta aagcataaaa aaaattaaat ttatttttaa
     1921 acataaatcg aaaggtctat cctttcaaaa tcttaaggca aacctcatca gaactttgcc
     1981 ttttttattt gaattttaga atgatttagt cggtacttta attgacagct tattttattt
     2041 tctttataat atatattgaa aatagcagtc caataagccg taaaaggctt ttttattaag
     2101 gaaaggaaaa cttatgttag attcttttga taagggttgg tttgtactcc aaacctactc
     2161 aggttatgaa aataaagtaa aggaaaatct cttgcaacgt gcgcaaactt ataatatgtt
     2221 agacaacatt ttgcgtgtgg aaatccctac tcaaactgta aatgttgaga aaaatgggaa
     2281 aacaaaagaa attgaagaaa atcgcttccc aggttatgta cttgtggaaa tggtaatgac
     2341 tgatgaagcc tggtttgttg ttcgtaatac tccaaacgtt actggatttg ttggatcaca
     2401 cggcaatcgc tctaaaccaa caccgttgtt agaagaagag attcgttcaa tactcatctc
     2461 aatgggacaa accgttgatg tctttgatac taatatcaag gaaggtgatg ttgttcaaat
     2521 cattgatggt gctttcatcg gacaagaagg acgtgtagtt gagattgaga ataacaaagt
     2581 taaattaatg atcaacatgt tcggctcaga aacacaagca gaacttgagt tgtaccaagt
     2641 tgcagaatta taaaacttct gaaaaatcca tcttaaaata aagatggatt tttgttaatt
     2701 tataattgat ttaggtttaa ttcgttaatt aaaacaacca tattatcaat agaattatga
     2761 ttaacgatat gtttggttat attctcttga tgagacgtgt tgttgaaaga ataaattggc
     2821 ttttgtttca aatgaactct ttgtaaaata ttacctactt ctggaccatg attaatatca
     2881 aggtatgcag tggctaatgt tattaatttt tcaattcttg cctcaattaa ttcagggtaa
     2941 actaggacat ttggatattg ttctagtttg ctaagtttat ttgacactgt tgtgtaggca
     3001 ccaatatgga attgaacagt aggacatcta caaattagcg attcaatgtg ttccaaatta
     3061 tcggaagatg taagggtgaa aagatgaata tctgaaacag aaagcaattc catggacttt
     3121 tcgtaagaaa tcacttttct ttgcttgccg gttaattggc aaaaatcaag ttggaagtaa
     3181 taccaccata gttctctaaa ccttgcaacg ctattttcat tccaaggttt gtcactagtt
     3241 aaaaaatgta taatattagg agttctattt tctatgttta gatataaata agatcgatga
     3301 ttatagcggc taacgacatc ataagtttga aagttccatg ttttgttcag agataaccaa
     3361 cgattatggc aaacaatgtt caagatactt tgatcccctg ttttgacaac tcggtggagt
     3421 tcttcagctg tttttagtaa tgttttagtg aggccatctt cccgccatag ttctagatta
     3481 gctaaaagca tgccagcatt aaaccctcct tcttcatttt tatggggatc tttgacggct
     3541 gctaagtaat gacctttaaa atctagttca aataagtaat caagattagt gttaacgata
     3601 atatctgaat ctaaataaag tacacgtgaa ctaggtatat aatctgctaa aaataatcta
     3661 aaccatgtac taacagttag aaaagtcgtt agatgagcaa tatcatagtt atatatttta
     3721 acattaatca gttttgatcc tagaggctct agtttcctgt ttatattacg tacccattca
     3781 actggggtat cttgattgaa taaataaaaa gttatatttc tattataaac acaaatagac
     3841 tttattgtta ctaaggcttt atccaagtag tttgaatcaa ctgcaagtgc aactgctttt
     3901 tccatttaaa ttctcctagt taagtgttta gaataatgtt gggtatatag tgaatggatg
     3961 ccaatttttt tatatagact gaagataatt aattaaatca tcgatagatg caaaaactgt
     4021 ttctggaaca agctggctat tgcattctct tgtagtaaga attggttttt cagattgtga
     4081 cattttctgt agaatgccct ccatttttcc aaaaacatta atatcaagat aaatatcagc
     4141 ttctgctaat aatttatcaa taataggtgg agtgtaagtt ttatgaaggc ggatattttc
     4201 aaaagctaat aggcttataa gaatgtcaga cataactgtt ggtgctgcaa tgtgaaatag
     4261 acaatctgga agggcagtga ctaattctga gatagatggt atacaatcag aagttgtaac
     4321 aataaggaaa ttgagacgat aattttgtgt tttaattggg tggtctaaac caaatacttt
     4381 tttactagat atgatacttg accattcaag taaattatag tgccaccata cctcacggag
     4441 tctcatattt gataatgttt gccaaggttt gattcccgaa atgtagtgaa taatttttgg
     4501 gagtggggta attgggatgt caaaaataaa tttatgccca tcgatagcag ctcccatatc
     4561 aaaaccaatt tgaaaattgt aggtatcatc taaataaatt acctgatttt caaacatgcg
     4621 attaagaatt gtttgatccc cttcgttggc attctcaatt tccttttgat ttttttcaat
     4681 taatttaata gtcatatttt cttgtcgcca acgttggttg ttaattacca ttactccagc
     4741 attaaaccca tatccaagcc caaaaagagc tggaactgca gctaaatatg cattgtctag
     4801 tgaaatttga aagaggttat ctaaattagt agtaacaact aagtcagagt caagatataa
     4861 aactgtatca gctgagatgt actgaggtat gaaataacga gcataggtca tataattaat
     4921 atgattaagc tttttatttt cccattttgg ggataagtga tcatggaaga gctttacatc
     4981 ctgaatttga tttccagttt gtccaaccct
//
LOCUS       DATRUK010000088         4944 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.088, whole genome shotgun sequence.
ACCESSION   DATRUK010000088 DATRUK010000000
VERSION     DATRUK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.088"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(103..1026)
                     /locus_tag="VBE34_001813"
     CDS             complement(103..1026)
                     /locus_tag="VBE34_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7264502.1"
                     /translation="MRIQQLQYVIKIVETGSMNEAAKQLYITQPSLSNAVRNLETEMG
                     IQIFIRNPKGITLTKDGMEFLSYARQILEQTALLEERYKGDNTSRELFSVSSQHYAFV
                     VNAFVALFNGTDMTQYELFLRETRTWEIIDDVKNFRSEIGVLFLNSYNRDVLTKLFDD
                     NSLIATTLFTTTPHIFVSKSNPLANRKKLSMKDLEDYPYLSYDQGLHNSFYFSEEMMS
                     QIPHPKSIVVSDRATLFNLMIGLDGYTVATGILNSKLNGDEIVAIPLDVDDVIDIVYI
                     RHDKANLSKMGQKFIDYLLEEVSFNHKNKQS"
     gene            1216..2673
                     /locus_tag="VBE34_001814"
     CDS             1216..2673
                     /locus_tag="VBE34_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO7264503.1"
                     /translation="MSNHSRRHQKKHSHTPLRVINLFLLVIFILLSVVSLFLMYRHHF
                     LAFRHLNVIYGVVIILIILASLFLCIKNKARIFTTIILVLSSIFVATTLYGFKSTIDL
                     TNNLNKTASYSEIEMSVVVPKDSKITNIEAVSKLAAPVKNDTSNITDLIEHIKSEKGI
                     SITPKKTDSYQDAYNRIKNGDSQAMVLNNAYVSLIELSTPDFKSQIKTIYTYKIKKKI
                     NRKNTNHKEGVFNIYISGIDTFGSISTVSRSDVNIIMTVNTNTHKVLLTTTPRDAYVK
                     IPDGGGNQYDKLTHAGLYGVETSMKTLENLYDINLDYYARINFSSFLKLIDLLGGVTV
                     YNDQAFTSKHGNFDFPVGQVTLNSEQALGFVRERYSLQGGDNDRGRNQEKVIAAIINK
                     LASSQSVTKLNSITSQLQTSVQTNMTIDNINDLINNQLSTGQRFTVESQALTGHGSTG
                     ELPSYAMPGAQLYMMSIDQSSLSNAKSKIKNTMEE"
     gene            2679..3410
                     /locus_tag="VBE34_001815"
     CDS             2679..3410
                     /locus_tag="VBE34_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEO7264504.1"
                     /translation="MIDIHSHIVFDVDDGPKTLEESLSLIEESYRQGVRIIVSTSHRR
                     KGMFETPEDIIFKNFSIVKHEAEKRFEHLQILYGGELYYTSDMLEKLKLKQIPTLNNT
                     KFALIEFSMQTSWKDIHTALSNVLMLGITPVVAHIERYNALENQKERVKEIINMGCYT
                     QINSFHILKQKLFNDKHKRFKKRARYFLEENLVHFVASDMHNLDVRPPFLAEAYKIIC
                     RDFGKERANQLFIENAQSILKNHYI"
     gene            3419..4111
                     /locus_tag="VBE34_001816"
     CDS             3419..4111
                     /locus_tag="VBE34_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEO7264505.1"
                     /translation="MNKIANTEVEINIFNLLKKLWKKKFLITFVAIAFATAGLFYSLF
                     IVTPQYTSSTRIYVINPNTPNNSITAQDLQAGSFLANDYKEIITSTDVLEKVISSEKL
                     NYPSSQLLQKITVSILKDTRVISISVEDANPKMSQKLANSVREAAVSKIKAVTQVEDI
                     TTLEKGNLPKAPSSPNIKKNVLIGFIVGAGLSTIVLVIMGILDDRVNTEEDIEKVLGL
                     TSLGIVPDLNKL"
     gene            4122..4811
                     /locus_tag="VBE34_001817"
     CDS             4122..4811
                     /locus_tag="VBE34_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEO7264506.1"
                     /translation="MTRLEIVDSKLRQAKKTEEYFNAIRTNIQFSGKENKILAIASVR
                     EGEGKSTTSTSLALSLAQAGFKTLLIDADTRNSVMSGTFKATGTIKGLTNYLSGNADL
                     GDIICETNVPRLMVVPSGKVPPNPTALLQNAYFNKMIEAIKNIFDYIIIDTPPIGLVV
                     DAAIIANACDGFILVTQAGRIKRNYVEKAKEQMEQSGSKFLGIILNKVNESVATYGDY
                     GNYGKRDRKRK"
BASE COUNT         1773 a          742 c          823 g         1606 t
ORIGIN      
        1 gcgaatcaaa gcaaaactaa aaggacttag tcctgtgcaa tacagaacta aatccttcac
       61 ttaaaataaa ttgtccaact tttgggggtc agtacaacaa tctcaggact gtttattttt
      121 atgattaaac gaaacttcct ctaataaata gtcaataaat ttttgtccca tttttgaaag
      181 gtttgctttg tcgtgtcgga tatagacaat atcaataaca tcatccacgt caagagggat
      241 tgcgacaatt tcgtcaccgt tcaattttga gttgaggata ccagttgcca ctgtataacc
      301 atctaatcca atcattaaat taaacagagt tgctctatca ctgacaacaa tggattttgg
      361 gtgaggaatt tgagacatca tttcctcaga aaagtagaaa gaattatgca ggccttgatc
      421 ataactcaga taaggataat cttctaaatc tttcatactt aatttttttc tattagctaa
      481 gggattggat ttgctcacga aaatatgagg tgtagtagta aaaagagttg tggcaattaa
      541 ggaattatcg tcgaataatt tggttaatac atctctatta tagctattaa gaaacagaac
      601 accaatctct gaacggaaat ttttaacatc atcaataatt tcccaagtcc tagtttcacg
      661 taaaaaaagt tcgtattggg tcatgtctgt gccattaaag agagctacaa aagcgttaac
      721 cacaaatgca tagtgttgtg aagaaacgct gaacagctca cgacttgtat tatcaccctt
      781 ataacgctct tcaagaagtg ctgtttgttc tagaatttgt cgcgcatatg acagaaattc
      841 cataccatct tttgttaacg ttattccttt tggatttcga ataaaaattt ggatacccat
      901 ctcagtctca aggttcctaa cagcgtttga aagtgaaggt tgggtaatgt agagttgttt
      961 agcggcttca ttcatgctac cagtttctac aatcttaata acatattgta attgttgaat
     1021 tctcataatt ctagtttaaa ctagaatgta aataaattca attagatcag ctaaattatc
     1081 aatatagatc aattgaaaat gttatttagc taatataatc agattattcc aatattattt
     1141 tcgttgaata ttgtattgaa aaatgatata atatccaagt aaaattaagg tttttttaag
     1201 tattggagaa ttacaatgtc taatcattcg cgccgtcacc aaaagaaaca ctcacataca
     1261 cctctacggg tgattaattt atttcttttg gtgattttta ttttgttaag tgtagtctca
     1321 ttatttctta tgtatcgtca ccattttttg gcatttagac acttgaacgt catttatgga
     1381 gttgtaatta ttttaatcat tttagcaagt ttatttcttt gtattaagaa taaagctaga
     1441 atttttacaa ctataatttt agtactatcc tctattttcg ttgctactac tttatatgga
     1501 tttaagtcaa ccattgattt gacaaataat ctaaataaaa ctgcttcata ctctgaaatt
     1561 gagatgagtg tagttgtacc aaaagattct aaaataacca atatagaagc tgtcagcaaa
     1621 ttagccgcac cagttaaaaa cgatacttca aatattactg atttgataga acatataaaa
     1681 tcagaaaaag gaatctctat tacaccaaaa aaaacagatt cttaccagga tgcatacaat
     1741 agaattaaaa atggtgatag tcaagctatg gttttaaata atgcttatgt tagcttaatt
     1801 gaacttagta cccctgattt taaatcgcag ataaaaacga tttatactta caaaattaag
     1861 aaaaaaatta atcgtaaaaa tactaatcat aaagaagggg tatttaatat ctatattagc
     1921 ggtattgata cttttggctc tatatcaaca gtatcaagat ctgatgtaaa tattattatg
     1981 acggttaata ccaataccca caaagtattg ttaacgacaa caccacgaga tgcctatgta
     2041 aaaattccag atggtggggg caatcaatat gataaattaa cccatgcagg tttgtatggt
     2101 gttgagacat caatgaaaac acttgaaaac ctttacgaca tcaaccttga ttattatgct
     2161 agaattaatt tttcatcatt tttaaaatta atagacctct tgggaggagt gacagtttat
     2221 aacgatcaag cttttacaag taaacatggt aattttgact ttcctgttgg tcaagtaaca
     2281 ttgaattctg agcaggcttt gggctttgtt agagaacgtt attctctaca aggaggcgat
     2341 aacgatagag gtagaaatca agaaaaagtg attgcagcta ttataaataa gttagcttct
     2401 agtcagtcag taacaaaatt aaatagcatt acctcacagc tccaaacgtc cgttcaaact
     2461 aatatgacta ttgataatat taatgatttg attaacaatc aattgtcaac tggacaacgc
     2521 ttcactgtcg agtcacaagc attaactggt catggttcaa cgggtgaact cccttcatat
     2581 gcaatgccag gagctcaact ttatatgatg tcaattgatc aatctagctt atctaatgca
     2641 aaatcaaaaa ttaagaacac aatggaggaa taacaagaat gattgatatt cattctcata
     2701 tcgtttttga tgtagatgat gggcccaaaa cgcttgaaga gagtttatct ttaatagaag
     2761 aaagttatcg acaaggtgtt agaataattg tttcgacatc acatagaaga aaaggtatgt
     2821 ttgaaacacc tgaagatatt atcttcaaaa acttttctat agttaaacac gaagcggaaa
     2881 aaagatttga acatcttcaa atcttatatg gtggagaatt atactataca agtgatatgt
     2941 tagaaaaact gaagttaaag caaattccaa ctttaaacaa tacgaaattt gctttaattg
     3001 aattttctat gcaaacttct tggaaagata ttcatacagc tttgtcaaat gttttaatgc
     3061 ttggtattac accagtcgtt gcgcatatag agaggtataa cgctttagag aatcaaaaag
     3121 aacgggtgaa ggaaattatt aatatggggt gttacacaca aataaatagt ttccatattt
     3181 tgaaacaaaa actttttaat gataagcata aacgctttaa gaaaagagcc cgttattttt
     3241 tagaggaaaa tttagtgcat tttgtagcga gtgatatgca taaccttgat gttagaccgc
     3301 catttttagc agaagcttat aagattatct gtagagattt cggtaaagaa cgtgctaacc
     3361 aactttttat tgaaaatgct caatctatat taaaaaacca ttacatttag gagatttcat
     3421 gaataaaata gctaatacag aagttgaaat aaatatattc aaccttttaa aaaaattgtg
     3481 gaaaaagaaa tttttaatca catttgttgc cattgcgttt gcaacagctg gacttttcta
     3541 tagtcttttt attgttacgc cacaatatac ttcttcaaca aggatatatg tcattaaccc
     3601 taatacccct aataatagta ttactgcaca agatctacaa gcggggagtt ttcttgcaaa
     3661 tgactataag gagattatta cgtctactga cgttctagaa aaagttattt cttctgaaaa
     3721 attgaattat ccttcgtctc agttgctaca aaaaataaca gtttctattt taaaagatac
     3781 acgtgttatt tcaatatcgg tcgaagatgc taatccaaaa atgtctcaaa aattagcaaa
     3841 ttcagttaga gaagcagcag tttcaaaaat caaggcagtt actcaagtag aggatatcac
     3901 tactcttgag aagggaaatt tacctaaagc accatcttct cctaatatta aaaagaatgt
     3961 actaatcggg tttattgttg gtgcaggatt atcaactatt gttttagtta ttatggggat
     4021 tttggatgat cgtgttaata ctgaagaaga tattgaaaag gtcttaggac ttacttcttt
     4081 aggtatagta ccagatttga ataaacttta aggagaatat aatgactcgt ttagaaatag
     4141 ttgatagcaa gttaagacaa gcaaaaaaaa cagaagaata cttcaatgcg atccgtacaa
     4201 acatacagtt tagtggaaag gaaaataaaa ttcttgcaat tgcctctgtt agggaagggg
     4261 aaggaaaatc cactacttca acaagtttag ctttatcttt agctcaagca ggatttaaaa
     4321 cattattaat tgatgcggat actaggaact ctgttatgtc tggaaccttt aaagcaactg
     4381 gaactattaa aggcttgacg aattatttat caggtaatgc agatcttgga gatattatct
     4441 gtgaaaccaa tgttcctaga ctgatggtcg ttccttcagg aaaagtacca ccaaatccaa
     4501 cagcattact tcagaacgct tattttaata agatgattga agctattaaa aatatatttg
     4561 attatattat catcgatact ccacctattg gtttagttgt tgatgccgca ataatcgcta
     4621 atgcttgcga tggttttatt ttagtaaccc aagcaggcag aataaaacgt aattatgttg
     4681 aaaaagcaaa agaacagatg gaacaaagtg gttcaaagtt cttaggtatt attcttaata
     4741 aagttaatga atctgttgct acttacggcg attatggaaa ttacggaaaa agggatagaa
     4801 aaaggaagta agggactctg gtattgaaag aaaaagaaaa tatacaaaag attattatag
     4861 cgatgattca aacagttgta gtttattttt ctgcaagttt gacattaaca ttaattactc
     4921 ccaattttaa aagcaataaa gatt
//
LOCUS       DATRUK010000089         4785 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.089, whole genome shotgun sequence.
ACCESSION   DATRUK010000089 DATRUK010000000
VERSION     DATRUK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.089"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..630
                     /locus_tag="VBE34_001818"
     CDS             <1..630
                     /locus_tag="VBE34_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0236 family protein"
                     /protein_id="HEO7264507.1"
                     /translation="ITNSDMGKGYTSRVFKELGKALKVKKHEHFWDIYHVKEKLSSYL
                     RKYPIELTDFALDAVKKYNSDKLELVFDTVESLICDELEDQEFQKFKKKVLNNFKYIK
                     PAHLRNLSNRGIGIMESQHRKITYRMKRRGMYWSKWGISTMANMIILERANGLRELFF
                     GSWRKVYSEYKEGSFSAGRLFKKTDELDKFSKPLLKNGRKWSITGIKTK"
     gene            1284..4724
                     /locus_tag="VBE34_001819"
     CDS             1284..4724
                     /locus_tag="VBE34_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO7264508.1"
                     /translation="MRKKQKLPFDKLAIALISTSILLNAQSDIKANTVTEDTPATEQA
                     VEPPQPIAVSEESRSSKETKTSQTPSDVGETVADDANDLAPQAPAKTADTPATSKATI
                     RDLNDPSHVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKENLEKAK
                     KEHGITYGEWVNDKVAYYHDYSKDGKNAVDQEHGTHVSGNAPSEMKEPYRLEGAMPEA
                     QLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDETKKAFD
                     YAKSKGVSIVTSAGNDSSFGGKPRLPLADHPDYGVVGTPAAADSTLTVASYSPDKQLT
                     ETATVKTDDHQDKEMPVISTNRFEPNKAYDYAYANRGTKEDDFKDVEGKIALIERGDI
                     DFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRRDGLLLKDNPPKTI
                     TFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAKLSGTS
                     MSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYFSPRQQ
                     GAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELYYQVTV
                     QTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMKNGYFL
                     EGFVRFKQDPTKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSDAKDQL
                     DGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGTFAKQD
                     DDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGNVVWTS
                     EVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPISSGAKE
                     QHTDFDVIVDNTTPEVATSATFSTEDSRLTLASKPKTSQPVYRERIAYTYMDEDLPTT
                     EYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLEGHSNK
                     PEQDGSDQAPDKKPEAKPEQDGSGQTPDKKKETKPEKDSSGQTPGKTPQKGQSSRTLE
                     KRSSKRALATKASTRDQLPTTNDKDTNRLHLLKLVMTTFFLGLAAHIFKTKRQKETKK
                     "
BASE COUNT         1692 a          918 c          934 g         1241 t
ORIGIN      
        1 atcactaact ctgatatggg taaaggctat actagtagag tttttaagga attaggaaaa
       61 gcacttaagg taaagaaaca tgagcatttt tgggatatct atcatgttaa agaaaagtta
      121 agttcatacc ttagaaaata tccaattgaa ttaaccgatt ttgctttaga tgcggtaaaa
      181 aaatataatt ctgataagct tgaattagtt tttgatactg ttgaatcact gatttgtgat
      241 gaacttgaag atcaagaatt tcagaagttt aagaaaaaag tattaaataa tttcaaatat
      301 ataaaaccag ctcatcttag aaatctttca aatcgtggta ttggtatcat ggaatcacaa
      361 cacagaaaga taacgtatag aatgaagcga cgtggcatgt attggtcaaa gtggggaatc
      421 tccacaatgg caaatatgat tatacttgaa agagctaacg gtttacgaga attatttttc
      481 ggttcttgga gaaaggtata cagtgagtat aaagaaggtt catttagtgc agggcgactt
      541 tttaaaaaga cagatgaatt agataaattt tctaagcccc ttctaaaaaa tggcagaaaa
      601 tggagtataa caggaatcaa aacaaaatag aaaaagtcta aagatacctt tatttctagc
      661 gtttttttgc atgttttaac ttgcattaaa tcttctaaag tagtataatt atattaaaga
      721 aaagcaaaaa aggtgaagga gcgcgaactc ctacaccaaa ccgattaaga caaataccac
      781 ttgcttaatc agtcgaacct tattgtagca taactaaaac aataccgtca agactgaaca
      841 agcaaaaaat acttgttttg gtctttttaa gaataattca agttttctta aatttatgag
      901 gtatcgacta atctcaaaat ttcagacaca tacctgagtg aacatcaaga gagaaccgat
      961 cggttctctc ttgatgtcta aaagaatgag gttaaggagg tcacaaacta aacaactctt
     1021 aaaaagatga cctttactaa taatcgtctt tgttttataa taaagatgtt agtaatataa
     1081 ttgataaatg agatacattt aatcattatg gcaaaagcaa gaaaaatagc tgtgtcatat
     1141 gcaaataacc cctgtttgct ctttaaaaaa gacgttatcc ttatttctct acacacagac
     1201 ggatagctag gagagaatcg tttgattctc tcttttctta atggtcataa agacaaagtc
     1261 tctcatcatg aaaggacgac acattgcgta aaaaacaaaa actaccattt gataaacttg
     1321 ccattgcgct tatatctacg agcatcttgc tcaatgcaca atcagacatt aaagcaaata
     1381 ctgtgacaga agacactcct gctaccgaac aagccgtaga acccccacaa ccaatagcag
     1441 tttctgagga atcacgatca tcaaaggaaa ctaaaacctc acaaactcct agtgatgtag
     1501 gagaaacagt agcagatgac gctaatgatc tagcccctca agctcctgct aaaactgctg
     1561 atacaccagc aacctcaaaa gcgactatta gggatttgaa cgacccttct catgtcaaaa
     1621 ccctgcagga aaaagcaggc aagggagctg ggaccgttgt tgcagtgatt gatgctggtt
     1681 ttgataaaaa tcatgaagcg tggcgcttaa cagacaaaac taaagcacgt taccaatcaa
     1741 aagaaaatct tgaaaaagct aaaaaagagc acggtattac ctatggcgag tgggtcaatg
     1801 ataaggttgc ttattaccac gactatagta aagatggtaa aaacgctgtt gatcaagaac
     1861 acggcacaca cgtgtcagga aatgctccat ctgaaatgaa agaaccttac cgcctagaag
     1921 gtgcgatgcc tgaggctcaa ttgcttttga tgcgtgtcga aattgtaaat ggactagcag
     1981 actatgctcg taactacgct caagctatca gagatgctgt caacttggga gctaaggtga
     2041 ttaatatgag ctttggtaat gctgcactag cttacgccaa ccttccagac gaaaccaaaa
     2101 aagcctttga ctatgccaaa tcaaaaggtg ttagcattgt gacctcagct ggtaatgata
     2161 gtagctttgg gggcaagccc cgtctacctc tagcagatca tcctgattat ggggtggttg
     2221 ggacacctgc agcggcagat tcaacattga cagttgcttc ttacagccca gataaacagc
     2281 tcactgaaac tgctacggtc aaaacagacg atcatcaaga taaagaaatg cctgttattt
     2341 caacaaaccg ttttgagcca aacaaggctt acgactatgc ttatgctaat cgtggtacga
     2401 aagaggatga ttttaaggat gtcgaaggta agattgccct tattgaacgt ggcgatattg
     2461 atttcaaaga taagattgca aacgctaaaa aagctggtgc tgtaggggtc ttgatctatg
     2521 acaatcaaga caagggcttc ccgattgaat tgccaaatgt tgaccagatg cctgcggcct
     2581 ttatcagtcg aagagacggt ctcttattaa aagacaatcc cccaaaaacc attaccttca
     2641 atgcgacacc taaggtattg ccaacagcaa gtggcaccaa actaagccgc ttctcaagct
     2701 ggggtctgac agctgacggc aatattaagc cggatattgc agcacccggc caagatattt
     2761 tgtcatcagt ggctaacaac aagtatgcca aactttctgg aactagtatg tctgcaccat
     2821 tggtagcggg tatcatggga ctgttgcaaa agcaatatga gacacagtat cctgatatga
     2881 caccatcaga gcgtcttgat ttagctaaga aagtattgat gagctcagca actgccctat
     2941 atgatgaaga tgaaaaagct tatttttctc ctcgccaaca gggagcagga gcagtcgatg
     3001 ctaaaaaagc ttcagcagca acgatgtatg taacagataa ggacaatacc tcaagcaagg
     3061 ttcacctgaa caatgtttct gataaatttg aagtaacagt aacagttcac aacaaatctg
     3121 ataaacctca agagttgtat taccaagtaa ctgttcaaac agataaagta gatggaaaac
     3181 actttgcctt ggctcctaaa gcattgtatg agacatcatg gcaaaaaatc acaattccag
     3241 ccaatagcag caaacaagtc accgttccaa tcgatgctag tcgatttagc aaggacttgc
     3301 ttgcccaaat gaaaaatggc tatttcttag aaggttttgt tcgtttcaaa caagatccta
     3361 caaaagaaga gcttatgagc attccatata ttggtttccg aggtgatttt ggcaatctgt
     3421 cagccttaga aaaaccaatc tatgatagca aagacggtag cagctactat catgaagcaa
     3481 atagtgatgc caaagaccaa ttagatggtg atggattaca gttttacgct ctgaaaaata
     3541 actttacagc acttaccaca gagtctaacc catggacgat tattaaagct gtcaaagaag
     3601 gggttgaaaa catagaggat atcgaatctt cagagatcac agaaaccatt tttgcaggta
     3661 cttttgcaaa acaagacgat gatagccact actatatcca ccgtcacgct aatggcaaac
     3721 catatgctgc gatctctcca aatggggacg gtaacagaga ttatgtccaa ttccaaggta
     3781 ctttcttgcg taatgctaaa aaccttgtgg ctgaagtctt ggacaaagaa ggaaatgttg
     3841 tttggacaag tgaggtaacc gagcaagttg ttaaaaacta caacaatgac ttggcaagca
     3901 cacttggttc aacccgtttt gaaaaaacgc gttgggacgg taaagataaa gacggcaaag
     3961 ttgttgctaa cggaacctac acctatcgtg ttcgctacac gccgattagc tcaggtgcaa
     4021 aagaacaaca cactgatttt gatgtgattg tagacaatac gacacctgaa gtcgcaacat
     4081 cggcaacatt ctcaacagaa gatagtcgtt tgacacttgc atctaaacca aaaaccagcc
     4141 aaccggttta ccgtgagcgt attgcttaca cttatatgga tgaggatctg ccaacaacag
     4201 agtatatttc tccaaatgaa gatggtacct ttactcttcc tgaagaggct gaaacaatgg
     4261 aaggcgctac tgttccattg aaaatgtcag actttactta tgttgttgaa gatatggctg
     4321 gtaacatcac ttatacacca gtgactaagc tattggaggg ccactctaat aagccagaac
     4381 aagacggttc agatcaagca ccagacaaaa aaccagaagc taaaccagaa caagacggtt
     4441 caggtcaaac accagataaa aaaaaagaaa ctaaaccaga aaaagatagt tcaggtcaaa
     4501 caccaggtaa aactcctcaa aaaggtcaat cttctcgtac tctagagaaa cgatcttcta
     4561 agcgtgcttt agctacaaaa gcatcaacaa gagatcagtt accaacgact aatgacaagg
     4621 atacaaatcg tttacatctc cttaagttag ttatgaccac tttcttcttg ggattagcag
     4681 ctcatatatt taaaacaaaa cgccaaaaag aaactaaaaa atagtattac ttaaactgat
     4741 aagagcttat ttaatagaga gaaccaattt ggttctctct atttt
//
LOCUS       DATRUK010000090         4567 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.090, whole genome shotgun sequence.
ACCESSION   DATRUK010000090 DATRUK010000000
VERSION     DATRUK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.090"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..1413)
                     /gene="gtfA"
                     /locus_tag="VBE34_001820"
     CDS             complement(<1..1413)
                     /gene="gtfA"
                     /locus_tag="VBE34_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEO7264509.1"
                     /translation="MVVYNLNRGIGWASSGVEYAQAYRSEVFRKLGVEAKFIFTDMFQ
                     NENIEHLTRNIGFEDNEIIWLYTFFTDLTIAATSYSLQQLKESFSLPIDRTEKNGKII
                     SFFFKGSSIVVTVMLNDESSNIVQRVEYLMGGKLVRKDYYSYTKMFSEYYAPEDIGPC
                     LYQRTFYNEDGSVAYEENVDGENSIFKFKETILYSKEELVGYMLEKLQLTNSDLILLD
                     RSTGIGQAVLRNKGNAKVAVVVHAEHYNVSATDETTILWNNYYDYQFSNADSIDAFIT
                     STETQTKTLIDQFKKYLNIEPVVYTIPVGSLSKLQRKEWHERKAFSLLTCSRLASEKH
                     IDWLINAVVEANKVIPELTFDIYGEGGERQKLQEIIAKNKANNYIRLMGHKNLSSVYK
                     DYQVYLSGSTSEGFGLTLMEAIGSGLPIIGLDVPYGNQTFIENNLNGYLIPRETPDNP
                     QQISTAFAQYIVALFNSKDIC"
     gene            complement(1429..3825)
                     /gene="secA2"
                     /locus_tag="VBE34_001821"
     CDS             complement(1429..3825)
                     /gene="secA2"
                     /locus_tag="VBE34_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEO7264510.1"
                     /translation="MKKVVVKPKISILNKLRLQRIQKIVNEINNKKVYYAQLSDKELS
                     HQTLILKNRLSSGESLDDILVDAFAVIREANKRILGLFPYDAQVMGAIALHQGTIAEM
                     KTGEGKTLTATMPLYLNALTSKGAILVTTNDYLAKRDALEMGKVYQFLGMTVGINVFD
                     KKEEADATIKREVYESDITYTTAGALGFDYLVHNLASNKLEQFLRPFHFVIVDEADSV
                     LLDIAQTPLIIAGDPRVQSNLYGITNNFVLTLKENDEYIHKAKDKIVYLTEKGVSYAK
                     QYFNISELYNDEYWELNRHINLALRAHCLYKRDYDYVVKNNEVKLLDNATGRVMEGTK
                     LQSGIHQAIEAKEDVALTNESRAIASITYQSLFNMFPRLSGMTGTGKSSEDELIKTYH
                     LPVIVIPTNFPIKRVDLADKIYVSLPEKLQATIDDVKQRHSKGQPVLLISGTVEIANI
                     YSKLLLREGIAHNTLTADNVVKEAQIIKEAGQKGSVTCATVLAGRGTDIKLGEGVREL
                     GGLAVIGTERMPNLRMDWQLRGRSGRQGDPGISQFYVSLEDELIVSHSPEWVIKYLKK
                     YSRKETSNYYMVPRKKRFFYQIVKNAQLRSEDKGVSSREQTIKFGESLRIQRENIYQL
                     RNELIADSSIVVDGVIKIIQDNFNDIANDKDLTEHSLRRYILENLTYKFKYFPDEFDV
                     HNSDDVFKLLIDIFDREFTAKKTKLQSDNEFDNFVRISILKAIDKSWIEEVDSLQQLK
                     GVVTNRGIGQRDIIQEYYKESLNSYYKMGKEIRYSIVKNIMLSTISKASDGSYSIYYV
                     "
     gene            complement(3822..4367)
                     /gene="asp3"
                     /locus_tag="VBE34_001822"
     CDS             complement(3822..4367)
                     /gene="asp3"
                     /locus_tag="VBE34_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEO7264511.1"
                     /translation="MIKKRIQKVLWTDSSNVYLYGSTIKYLEHNRVFFENLRFSTGKT
                     IKKWLSKTNFQANRTLPELTLLKPNHTYELVSKLTTVPEGCVIIQINFYNRQKEKIGF
                     SIIRDHSSDFIVPDDTYFYDISLIGAGCISVEFCYLELFEVIETQEEKDGIELNSYVE
                     SSLPSELELIRTLIAFSKDKK"
     gene            complement(4360..>4567)
                     /locus_tag="VBE34_001823"
     CDS             complement(4360..>4567)
                     /locus_tag="VBE34_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEO7264512.1"
                     /translation="FAISYMKHDDYDRNATPRLIDYFSKTNTLLYASGYAGRHNDNSQ
                     SINNWFTSQYKTILSDDFGRVFSD"
BASE COUNT         1458 a          880 c          601 g         1628 t
ORIGIN      
        1 acaaatatct ttagaattaa ataaagcaac aatatactga gcaaaagcag ttgatatttg
       61 ttgaggatta tctggtgttt cccttggaat aaggtagcca ttcaaattat tttcaataaa
      121 ggtctgatta ccatacggaa catctaaacc aattatcgga agccccgatc cgattgcctc
      181 catcaaggtt aaaccaaaac cttcactggt agaacccgat aaataaactt gataatcttt
      241 ataaacagaa gataggtttt tatgccccat taatcgtata taattatttg ccttattttt
      301 agcaattatt tcttgtaact tttgccgttc gccaccttca ccataaatat caaaggttaa
      361 ttcaggaatt actttattag cttctaccac agcattaatt aaccaatcaa tatgtttttc
      421 cgaagctaga cgtgagcaag ttaacaacga aaaagctttt ctttcatgcc actcttttct
      481 ctgtaatttt gataaacttc ctacaggaat tgtataaacg accggttcaa tatttaaata
      541 ctttttaaat tgatcaatca acgtcttagt ttgcgtttct gttgaggtga taaaagcatc
      601 tatgctatca gcatttgaaa attgatagtc ataataatta ttccataaaa tcgtagtttc
      661 atcagttgcg ctaacattat aatgctctgc atgaacaaca acagctactt tagcatttcc
      721 tttattcctt agaacagctt gcccaatgcc tgtagagcgg tctaacaaga ttagatcact
      781 attcgttaat tgtaattttt ctaacatgta tccaactaat tcttctttag aatatagtat
      841 agtttctttg aatttaaaaa tactattctc gccatctaca ttttcttcat atgcaacact
      901 tccatcttca ttataaaatg tacgctgata caggcatgga cctatatctt cgggagcata
      961 atattctgaa aacattttag tatatgagta gtagtctttt ctaacaagct ttccccccat
     1021 aagatactct accctttgga cgatgttact agattcatcg ttaagcataa ctgtaactac
     1081 aatactactt cctttaaaaa aaaatgatat tattttaccg tttttctcag tcctatcaat
     1141 cggtagtgaa aaactttctt tcaattgttg cagtgaataa gaagttgcag caattgttaa
     1201 atcagtaaaa aaagtataaa gccatataat ctcattgtct tcaaagccga tatttcgagt
     1261 aaggtgctct atattttcat tctgaaacat atcagtaaaa ataaatttag cttcaacacc
     1321 taactttcta aaaacttctg aacgatatgc ttgagcatac tctacaccgc tacttgccca
     1381 acctatccct ctattcaaat tataaactac cattaactcc gttttccttt atacataata
     1441 tattgagtaa cttccatcac ttgcttttga aattgtactt aacataatat tcttaacgat
     1501 agaatatctt atttcttttc ccatcttata gtaagaatta agcgattctt tataatattc
     1561 ttgaataata tctcgttgac cgatacctct attggttaca actcccttta attgctgtag
     1621 gctatccact tcttctatcc acgatttgtc aatagccttt aatatcgata ttctgacgaa
     1681 attatcaaat tcattatcac tttgaagttt agttttcttt gcagtaaatt ctctatcaaa
     1741 tatgtcaatc aataatttaa atacatcgtc agaattatga acgtcaaatt catcagggaa
     1801 atatttaaat ttataggtaa gattttctaa tatatatctt cgtaaagaat gttccgttaa
     1861 atccttatca ttagcaatat cgttaaaatt atcctgaata atttttatca caccatcaac
     1921 cacaatggaa ctatcagcta ttaattcatt tcgtaattga taaatatttt cacgctgtat
     1981 acgcaagctc tctccgaact taatagtttg ctccctagaa gatacacctt tatcttcact
     2041 tctgagttga gcatttttaa ctatttgata aaaaaagcgt ttctttctag gtaccatata
     2101 gtaatttgaa gtttctttac gagaatattt ttttaaatac tttatcaccc attccggaga
     2161 atgagaaaca attaattcat cctctagcga cacataaaat tggctaatcc ctgggtctcc
     2221 ttgacgcccc gaacggcccc taagctgcca gtccatacgt aaattaggca tcctttccgt
     2281 tcctataact gcaagtcctc ctaattctct aaccccttca cctagtttaa tatctgttcc
     2341 acgaccagca agaactgttg cacatgttac tgaacctttt tgtcccgctt cttttataat
     2401 ttgtgcctct ttaacaacat tatcagctgt taaggtatta tgggcaatgc cttctcgcag
     2461 caataacttt gaataaatgt tagcaatttc aactgttcca gaaattaaca atactggctg
     2521 acctttacta tgtctttgtt taacatcatc tatagtagct tgcaactttt ctggtaagct
     2581 aacatatatt ttatcggcaa gatctactcg tttgatcgga aaatttgttg gtataacaat
     2641 aacaggcagg tggtaagttt ttataagttc atcttcagaa gattttccag ttcctgtcat
     2701 cccagacaac cttgggaaca tattaaagag actttgatac gttattgaag caattgccct
     2761 agattcattt gtcaaagcaa catcttcttt tgcttcaata gcttggtgaa tccctgattg
     2821 aagttttgtg ccttccataa cacgacctgt tgcgttatct aacaatttta cttcattatt
     2881 tttaactaca taatcataat ctcttttata caaacaatga gcacgcaatg ctaggtttat
     2941 atgtctattt aattcccaat actcatcatt atataattct gaaatattga agtactgttt
     3001 tgcgtaactt acacccttct cagtaagata gacaatttta tccttagctt tatggatata
     3061 ttcatcattt tccttaagag tgagtacaaa attattagtg ataccataga gattagattg
     3121 tacacgagga tctcccgcta taattaaggg tgtttgagca atatctaaca aaacagagtc
     3181 tgcttcatca acgataacaa aatgaaaagg tcttagaaat tgttctagtt tattactagc
     3241 cagattgtga acgagatagt cgaaacctaa ggcacctgca gtagtataag taatatcaga
     3301 ttcatatact tctctcttaa tagtcgcatc tgcttcttct tttttatcaa aaacattaat
     3361 accaactgtc atacctagaa attgatatac ttttcccatc tctaatgcat ctcgttttgc
     3421 caaataatca ttggtcgtca ctaagattgc tcccttgcta gttaaagcat tcagatacaa
     3481 gggcatagtt gcagttaatg tttttccttc tcccgttttc atttcagcta tcgttccttg
     3541 atgaagagca atagcaccca taacctgagc atcatatgga aacaaaccta aaattctttt
     3601 attagcctct cttatcacag caaaagcatc aacaagaata tcatctagac tttctccact
     3661 tgaaagtcta ttttttaata ttaaagtttg atggcttaac tccttatctg ataattgagc
     3721 gtagtacact tttttattat ttatctcgtt tacaattttt tgaattcttt gcaatctcaa
     3781 tttatttaga atagagattt tgggtttaac aacaactttt ttcatttttt atccttagaa
     3841 aatgctatca acgttctaat aagttcaagc tctgatggta agctgctttc aacataactg
     3901 tttagttcaa taccatcttt ttcttcttga gtctcaatta cttcaaataa ttctaggtaa
     3961 caaaattcta cacttataca acccgcacca ataagagaaa tatcataaaa atatgtgtca
     4021 tcaggaacta taaaatctga ggagtgatct cgaataatag aaaaacctat tttttctttt
     4081 tgacgattat aaaaatttat ctgaataatc acgcatccct caggcacagt agttaatttt
     4141 gataccaact cataggtatg attaggtttt agtaaggtaa gctctggcaa cgttctatta
     4201 gcttgaaaat tcgttttaga aagccatttt ttgattgttt ttcctgtaga gaatcttaga
     4261 ttctcaaaaa aaacacgatt atgctccaaa tactttatag tcgaaccata aagataaaca
     4321 ttgctactat cagtccaaag caccttctgt attctttttt taatcactaa acactctccc
     4381 aaaatcatca ctgagaattg ttttatactg agaagtaaac caattattta ttgattgact
     4441 attatcatta tgtcgcccag catatccact tgcatacaac agggtgttag ttttagaaaa
     4501 ataatcaatc agccttggag ttgcatttct atcataatca tcatgtttca tataagatat
     4561 agcaaac
//
LOCUS       DATRUK010000091         4509 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.091, whole genome shotgun sequence.
ACCESSION   DATRUK010000091 DATRUK010000000
VERSION     DATRUK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.091"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            155..1185
                     /locus_tag="VBE34_001824"
                     /pseudo
     CDS             155..1185
                     /locus_tag="VBE34_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767962.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
     gene            complement(1396..2208)
                     /locus_tag="VBE34_001825"
     CDS             complement(1396..2208)
                     /locus_tag="VBE34_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO7264513.1"
                     /translation="MLLDMLSYDFMQRALLAVVAISIFAPILGIFLILRRQSLMSDTL
                     SHVSLAGVALGVVLGISPTWSTIFVVTLAAVVLEYLRTVYKHYMEISTAILMSMGLAI
                     SLIVMSKAHNVGNVSLEQYLFGSIITIGKEQVIALFVIALITFILTILFIRPMYILMF
                     DEDTAFVDGLPVRTMSILFNVVTGIAIALTIPAAGALLVSTIMVLPASIAMRLGRNFK
                     TVIFLGMLIGFVGMVAGIFLSYYWETPASATITMIFIGIFLLVSLVGLLRKR"
     gene            complement(2198..2908)
                     /locus_tag="VBE34_001826"
     CDS             complement(2198..2908)
                     /locus_tag="VBE34_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO7264514.1"
                     /translation="MRYITVSGLTFQYDSDPVLEGVNYHLDSGEFVTLTGENGAAKST
                     LIKATLGILTPKVGTVNISKENKEGKKLRIAYLPQQIASFNAGFPSSVYEFVKSGRYP
                     RNGWFRRLTKHDEEHIRVSLEAVGMWDNRHKKIGSLSGGQKQRAVIARMFASDPDIFV
                     LDEPTTGMDAGTTEKFYELMHHNAHKHGKSVLMITHDPDEVKGYADRNIHLVRNQSLP
                     WRCFNVHTNEMEVESDAT"
     gene            complement(2911..3354)
                     /locus_tag="VBE34_001827"
     CDS             complement(2911..3354)
                     /locus_tag="VBE34_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEO7264515.1"
                     /translation="MTVLEQKLDHLVSQILLKAENQHELLFGTCQSDVKLTNTQEHIL
                     MLLSQEQLTNSDLAKKLNISQAAVTKAVKSLISQDMLKANKDSKDARITYFELSELAK
                     PIADEHTHHHDNTLGVYGRLVNHFSKDEKVVLERFLDLFSRELEG"
     gene            complement(3441..4292)
                     /locus_tag="VBE34_001828"
     CDS             complement(3441..4292)
                     /locus_tag="VBE34_001828"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEO7264516.1"
                     /translation="MKIFEKAPAKLNLGLDIKGRCDDGYHELAMIMVSIDLNDYVTIS
                     ELKEDCIVIDSDSSKMPLNNDNDVFKAADIIKNQYGINKGVHIRLEKSIPICAGLGGG
                     STDAAATIRALNRLWNLQMGHDEMVAIGFKIGSDVPYCLGGGCSLVLGKGEIVKPLPT
                     LRPCWIVLVKPDFGISTKSIFRDIDCKSISRVDIDLLKSAILSSDYQLMVKSMGNSLE
                     DITITKNPVISTIKERMLNSGADVALMTGSGPTVFSMCSTEKKADRVFNSMKGFCKEV
                     YKVRLLR"
     gene            complement(4432..4505)
                     /locus_tag="VBE34_001829"
     tRNA            complement(4432..4505)
                     /locus_tag="VBE34_001829"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4469..4471),aa:Asn,seq:gtt)
BASE COUNT         1654 a          888 c          657 g         1310 t
ORIGIN      
        1 ctataacatt taactttaat aactatctag ttaaataaca taatctccaa aaaaaattaa
       61 ctagttatgt ttttttatta ctttttccca ataaaatgat aaaattatac ttgtgaaagg
      121 ccttcacaaa tatcatttag aagggtttag gtttatgaaa ttatctaaac aattatacaa
      181 atcactccca ctattaactg tcgtactatg cgttggggca ttacaacaga atgttgaagc
      241 taaggctaaa cattataaga cgacatccca tgttgagaca cagtatgtaa gtacttcttc
      301 aaaaaaatct taccgtttac tcataataag caaatcaagg taggttcatt agataactta
      361 gaaagagcaa catattctca catacaatta agggatgcag atgagcctaa gataaaaaga
      421 gaaagactga cgtataatcc aaccggttgg cacaactaca aatttactac tgaaaagggt
      481 aaaacaactt ggcttataga ccgaggtcac ctcgttggtt atcaattttc aggaatgaac
      541 aatgttcctg agaacctagt tactatgacc aaatatctaa atacaggatt tagcgaaaat
      601 aatcctgatg gtatgttata ctacgaaaat agattagata gttggttggc aaatcataaa
      661 aatttctagc ttgattacaa agtgactcca atatacgaag gaaataattt agtacctagc
      721 cgagttgaac ttcagtatgt tggaattgat aaacaaggaa aacttctaga aatcaaatta
      781 ggcggcggca aagaacaagt tgatgaatac ggcgtcacaa ctgttacatt agaaaatacg
      841 tctccactcg caaaaattga ttacaaaact ggtatgctaa tcaaagaaga tgggaaacag
      901 gcagaagaag gtgaagatcc aaatagcgat gctgatgaaa acgaagcagc tattgaaggt
      961 gcatctgata tagaagaaaa tgctaacact aatacttctg aatcagatac aaataatgta
     1021 gctcccaaaa atagaatcgt ctacgttgca aataagggtc gttcaaatac ttattggtat
     1081 agtttagaaa acataaaaaa tgcaaatacc gctaatatcg ttcaaatgac tgaacaagaa
     1141 gcgttaaatc aacacaaata tcacagcact actgaagcac aataattaca atttactgcc
     1201 ggcaaaattg ccggtagtat tttttatcct aaaacgcaaa aatagattca cattgcccct
     1261 tctatcaact taagccagaa aacttagtag atacctcatc gagataattt ctcaactgat
     1321 ccctcataaa atatattcga aaaaaacagg gagataaata aaaaatctat cctagaataa
     1381 gatagatttt tttatttaac gtttgcgaag caagcctact aagctaacta ataggaaaat
     1441 gccaataaaa atcatcgtta ttgttgcact agcaggtgtc tcccaataat aagaaaggaa
     1501 aataccagct accattccca caaatccaat taacatccca agaaaaataa cagttttaaa
     1561 atttcgacct aaccgcatag caatactagc tggtaaaacc ataatagttg acaccaatag
     1621 agcacctgcg gcaggaattg ttaacgcaat agcaatacct gtaacaacat tgaacaaaat
     1681 agacattgta cgcacaggca atccatctac aaaagctgta tcctcatcaa acattaatat
     1741 atacattggt ctaataaata ggatggttaa aataaaagtg attaatgcaa taacaaacaa
     1801 agcaatcact tgttccttcc caatagtgat aatagaacca aataaatact gttcaagact
     1861 gacattacca acattatgag ctttactcat aacgattaaa gatatagcca accccatcga
     1921 catcaggata gcagttgata tttccatata atgtttgtat acagtacgca aatactctaa
     1981 tacaactgcc gccaaagtaa caacgaaaat agttgaccac gtaggcgaga tacctaaaac
     2041 aacgcctaat gcaacaccgg ctaaggaaac gtggctcaaa gtatcactca tcaaactctg
     2101 acgacgtaaa attaggaaga tgcctaaaat tggagcaaaa atactaatag ccacaactgc
     2161 taagagcgct cgttgcataa aatcatagga aagcatatca agtagcatct gattcaacct
     2221 ccatttcgtt tgtatggaca ttgaaacaac gccatggtaa agattggttc ctaacaagat
     2281 gaatatttcg gtcagcatac ccctttactt catcaggatc gtgagtaatc atcaaaacag
     2341 acttaccatg cttatgagca ttatgatgca ttaactcata aaatttctca gtagtccctg
     2401 cgtccatacc agttgttggc tcatccaata caaaaatatc tggatcagaa gcaaacatcc
     2461 tagcaatgac agccctctgc ttttgaccgc cagataaact tcctatcttt ttatggcgat
     2521 tatcccacat accaacagct tctaaactaa ccctaatatg ttcttcatca tgtttagtca
     2581 gtcttctaaa ccaaccattt ctagggtatc gtccagattt cacaaattca taaactgacg
     2641 atggaaagcc agcgttaaaa cttgcaatct gttgaggtaa ataggcaata cgtaattttt
     2701 ttccttcttt gttttcttta gagatattaa ctgtacccac tttgggcgtt aaaataccta
     2761 aagtagcctt tattaaagtt gatttagcag ccccattttc acctgttaag gtaacaaact
     2821 ccccactgtc taaatggtaa ttaacccctt ccagcacagg atcgctatca tactgaaaag
     2881 taagaccact aactgtaata tatcgcatga ttacccttct aattctctag agaaaagatc
     2941 aagaaaacgt tctaaaacga ccttttcgtc ctttgaaaaa tgatttacta atcttccgta
     3001 aacccctaac gtattgtcat gatgatgtgt gtgttcatct gcaatgggtt tagcaagttc
     3061 agataactca aaataagtaa tacgagcatc tttagaatct ttattcgctt tcaacatatc
     3121 ctgagaaatt aaacttttta ctgctttagt tacagctgcc tgactaatat ttaacttctt
     3181 agctaaatca gaatttgtca actgctcttg tgataaaagc atcagaatgt gttcttgcgt
     3241 attagtcaat tttacatcac tttgacaagt accaaacaat aattcatgtt gattttctgc
     3301 cttaagcaag atttgactca ctaaatggtc taatttttgt tctaaaactg tcatatatac
     3361 ctcttttttt gttaaccagt aaattatatc acgaagatat agaagaatca atcatagata
     3421 ggtgaagaag ataaaacctt ttatctcaac aacctaactt tataaacttc tttgcaaaaa
     3481 cctttcatac tattaaaaac acgatcagct tttttctctg tagaacacat tgaaaaaaca
     3541 gttggtccac ttcctgtcat taatgcaaca tcggctccag aatttaacat acgttctttt
     3601 attgtactta taactggatt tttagtaatt gtaatatcct cgagtgaatt tcccatagat
     3661 ttgaccatta actgataatc tgatgacaaa atagcagact ttaataaatc aatatcaact
     3721 ctgcttatag acttacaatc aatatctcta aaaatggatt tagttgaaat accaaaatcc
     3781 ggcttaacca gaactatcca acatggtctc aatgtcggta agggtttaac aatttcacct
     3841 ttacctaata ctaacgaaca tcccccacca aggcaataag gaacatcact accaatttta
     3901 aaaccaatag caaccatttc gtcatggccc atttgaagat tccataatcg attaagagct
     3961 cttattgtag cagcagcatc agtagacccc ccccccagtc ctgcacagat aggaatggat
     4021 ttttctaatc taatatgaac acctttatta ataccatatt gatttttgat tatatctgca
     4081 gctttaaaca catcattatc attatttaaa ggcattttgc tactatcaga atcgataaca
     4141 atacaatctt cctttagctc agaaatggta acgtagtcat taagatcaat actaaccata
     4201 atcatagcta attcatgata accatcgtca catcgtcctt taatatctaa tcctaaatta
     4261 agtttggcag gagctttctc aaaaattttc ataaaacctc cctaataaaa tatagaatat
     4321 ccatattata acataacaaa tgacaaattc ggacaattaa gatgttagcc gaccttaagg
     4381 aataataatt ccaataaaaa aaggctaacc aaagttagtc tcctttatct actccgccag
     4441 taggactcga acctacgaca tcatgattaa cagtcatgcg ctactaccaa ctgagctatg
     4501 gcggattat
//
LOCUS       DATRUK010000092         4494 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.092, whole genome shotgun sequence.
ACCESSION   DATRUK010000092 DATRUK010000000
VERSION     DATRUK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.092"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(27..191)
                     /locus_tag="VBE34_001830"
     CDS             complement(27..191)
                     /locus_tag="VBE34_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264517.1"
                     /translation="MLLTSIDVNPVYANMAFDWVYDNYKDDIINYGQETIKNIGKSIS
                     ANNSLGTLYN"
     gene            complement(493..945)
                     /locus_tag="VBE34_001831"
     CDS             complement(493..945)
                     /locus_tag="VBE34_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEO7264518.1"
                     /translation="MNRLSWEDYFMANAELISKRSTCDRAFVGAVLVKNNRIIATGYN
                     GGVSETDNCNEVGHYMEDGHCIRTVHAEMNALIQCAKEGISTNNTEIYVTHFPCINCT
                     KALLQAGVKKITYKANYRPHPFAIELMEAKGVAYVQHDVPEVTLGMEE"
     gene            complement(957..2024)
                     /locus_tag="VBE34_001832"
     CDS             complement(957..2024)
                     /locus_tag="VBE34_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO7264519.1"
                     /translation="MGFEQRLTRCQTAISQLSCDALLITNLTNIFYLTGFSGTNATVL
                     ISPKHRIFVTDSRYALIAKNTVREFDIIISREPLAAILKIIRDDALIAIGFETDISYH
                     MYKHMVEVFEDYRLIEAPSVVEKLRMIKDVTELAIIRQACRISDQAFLDVLDFIKPDR
                     TTELQVANFLDFRMRELGATGPSFDFIVASGYRSAMPHGVASQKTIQSGETLTLDFGC
                     YYQHYVSDMTRTIHIGHVTDQEREIYDIVLKSNQAIIDNVKSGMKRCDYDYLARQVIE
                     NSGYGNHFTHGIGHGMGLDVHEIPYFGKSEGVIASGMVVTDEPGIYLDNKYGVRIEDD
                     LLITETGCEVLTSAPKELIVL"
     gene            complement(2124..2840)
                     /locus_tag="VBE34_001833"
     CDS             complement(2124..2840)
                     /locus_tag="VBE34_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEO7264520.1"
                     /translation="MIILIIALATILTFVTWMLIRPKALRYILGLLSLALLFASVWML
                     TDHFINHTGMTIETKTKTREIYSAGDSKAPFGLMIYKEIGTHSSNNVLVYRTDLNQKE
                     AKAHFVPNTKEVTSAVKKSASYQLADVKKATVTTKTKRYVWKDSLSKLLYGFAGENGE
                     LISQKSVAKIPRKTWLVLTKEQSEHLESAAAKLKEQMTSQAKVNPVKAMEIKTLAKTN
                     PEAYAEMQVKALKEALNIKE"
     gene            complement(2842..4341)
                     /locus_tag="VBE34_001834"
     CDS             complement(2842..4341)
                     /locus_tag="VBE34_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO7264521.1"
                     /translation="MTTKAKHDIHGKPYNRTAMITLLLIATFAGVLNQTSLGTAIPTL
                     MNSFNISLSTAQQATTWFLLANGIMIPVSAYLATRFSTKWLYVTSYVVLLIGLLMTTL
                     APTSNWNLFLVGRIIQAISVGISMPLMQVVMVNVFPPEQRGAAMGLNGLVVGLAPAIG
                     PTLAGWILKQEFHFAGHDLTWRAIFLLPLLILTVTTILSPFVLKDVVDNKSVKLEVPS
                     LILSIIGFGSFLWGFTNVATYGWGDIGYVISPIMVGIIFIALFIHRQLKLETPFLDIR
                     VFKNKQFSVTTAAIALSMMAMMGVEMMLPLYLQNVHGLSALDSGLALLPGALMMGIVS
                     PISGAVYDKVGARRMAMIGFTILGVATLPFVFLTTTTPDHFITLLYAVRMFGIAMVMM
                     PLTASAMSALPPHEAAHGTAANNTARQIASAVVVALLSSVAQNIITNNKPSKDLLTMN
                     PLKYANQMLNASLDGFHVSFAIGFVFAVLGLLVSLFLRKGKIIETEKEV"
BASE COUNT         1471 a          899 c          696 g         1428 t
ORIGIN      
        1 ctatttccgt tttcaaatct tgctccttaa ttatataaag tacctaaact attattagct
       61 gaaatggatt taccaatatt tttgatagtt tcttgtccat aattgatgat atcatcttta
      121 tagttatcat acacccaatc aaatgccata ttagcataaa ctggatttac atctattgag
      181 gtcagcaaca atatcttttc tcttttttgt aaattatttt tgcaattcga gattcccagc
      241 agtactaatt ttagatgttg ataatagatg gatcagctat tctattatca acatctaaaa
      301 ctttctgtga aaaatcatta ggaataatga ctattaactg gtgatcacct tttttaggcc
      361 attttcagta atcccatgac taacaattga ccaattttga gaattatctc gttcaatata
      421 tttaacataa ctggccctta gcttaaacga taaaaccttc aaggaagata ggcatatact
      481 ctatcttcct ctttactctt ccatccctag tgttacctct ggaacatcat gttgaacata
      541 agcaactcct tttgcttcca taagttcaat agcaaatggg tgtgggcgat aattagcttt
      601 ataagtaatt ttcttaaccc cggcttgcaa gagcgccttg gtgcaattga tacaaggaaa
      661 atgcgtcaca taaatttctg tattattagt agaaatacct tctttagcac actgaataag
      721 ggcattcatc tcagcatgaa cagtcctaat acagtgacca tcttccatat aatgtcccac
      781 ttcgttacag ttatctgtct cagagactcc tccgttataa cctgtagcaa tgatgcgatt
      841 atttttgact aaaacagctc caacaaaagc acggtcacag gtagaacgtt tagaaatcaa
      901 ctctgcattt gccataaaat aatcttccca agaaagtcta ttcattctat tttcctttaa
      961 agtacaatta attctttcgg tgcagatgtc aatacttcgc aacctgtttc cgttattaat
     1021 aaatcatctt caatacgaac accgtattta ttatcgagat atattcctgg ctcgtcagtc
     1081 actaccatcc cagatgcaat cactccctct gacttaccaa aataaggaat ttcatgaaca
     1141 tcaagcccca taccatgccc tataccgtgg gtaaaatgat taccgtatcc agaattttca
     1201 atgacttgtc gcgccaagta atcataatca caacgcttca ttcctgattt tacattatca
     1261 ataatagctt gattactttt caaaacgata tcgtaaatct cgcgttcctg atcagtgaca
     1321 tgaccgatat gaatcgtacg tgtcatatca ctaacatagt gctgatagta acaaccaaaa
     1381 tctaaagtca gagtttctcc cgattgaata gtcttctgac tcgcaacccc atgtggcatc
     1441 gctgatcgat atccagatgc tacaataaaa tcaaatgaag gaccagttgc tcctaattct
     1501 ctcatgcgaa agtctaaaaa attagcaact tgcaattcag ttgttcgatc aggctttata
     1561 aaatccaaca catctaaaaa agcttggtcc gaaatacgac aggcttgacg gatgatcgca
     1621 agttctgtta catctttaat catacgtaac ttttcaacaa cagatggagc ttctatcaag
     1681 cgataatctt caaaaacttc caccatatgt ttgtacatat gataagaaat atcagtttca
     1741 aaaccaatcg ctatcaatgc gtcatcgcga ataattttca aaatagccgc aagtggttct
     1801 ctgctaatga taatatcaaa ctctcttaca gtattctttg ctatcaaggc atatcgtgaa
     1861 tctgtaacaa aaatccggtg ttttggacta atgagtaccg ttgcattagt tcctgaaaaa
     1921 ccagtcaaat aaaaaatatt tgttaaattt gttattaaga gtgcgtcaca tgataattgt
     1981 gaaattgctg tttgacatct agttagacgt tgttcaaaac ccataagaaa cctcgctatt
     2041 ctaaccttaa ttaggttaat tttatcatct tttaaaacat ttagctataa gaaaagcaac
     2101 tcatttcagt tgcttttact ttactattct tttatattaa gagcttcttt taatgcttta
     2161 acttgcatct cagcataagc ttctggattt gtcttagcta atgtctttat ctccatagct
     2221 ttcactgggt ttacttttgc ttgcgaagtc atttgttctt ttagtttagc cgcagccgat
     2281 tctaagtgtt cggactgctc tttagtcaaa actagccaag tttttcgtgg tattttagca
     2341 actgatttct gagaaatcaa ttctccattt tcacctgcaa agccataaag tagtttcgaa
     2401 agactatctt tccacacata acgtttagtc tttgttgtta ctgtggcttt tttcacatca
     2461 gctaattggt agctagcaga ttttttcaca gcacttgtca cttcttttgt atttggaaca
     2521 aagtgagctt ttgcttcttt ttgatttaaa tctgttcgat aaactaaaac attattactc
     2581 gaatgagtac caatctcttt ataaatcatg agaccaaatg gagccttact atcacccgca
     2641 ctataaatct cacgtgtctt tgtcttagtt tcaatagtca tacctgtatg attaataaag
     2701 tgatccgtta acatccaaac tgaggcaaac aacaaagcta atgagagaag acctagaata
     2761 taccgtagag cttttggacg aatcaacatc catgtgacaa atgtcaaaat agttgccagt
     2821 gcaataatca aaataatcat gctatacctc cttttccgtc tcaataattt tcccttttct
     2881 caggaaaaga gataccagta aaccaagaac cgcaaataca aaaccaatgg caaaagaaac
     2941 atggaagcca tcaagactag cattcaacat ctgatttgca tattttaaag gattcattgt
     3001 tagcaaatct tttgatggtt tattgttggt gataatattt tgagcaacac ttgaaagcaa
     3061 tgcaactaca actgctgaag caatttgacg agcagtatta ttagctgctg ttccatgtgc
     3121 ggcctcatgt ggtggaagtg cgctcatcgc acttgctgtc aatggcatca taaccatagc
     3181 aataccaaac atacgtactg catataagag ggtaataaaa tgatctggtg ttgtagtcgt
     3241 taagaaaaca aaaggtaacg tcgctacacc tagtatggta aaaccaatca tagccatacg
     3301 tcttgcacca actttatcat atacagctcc agaaattgga ctaactatcc ccatcatcaa
     3361 ggcacctggt aataaagcta aaccagaatc aagtgcagag agaccatgaa cattttgcaa
     3421 gtaaagcggt agcatcattt caacacccat catcgccatc attgaaagtg caatagctgc
     3481 tgttgttact gagaattgtt tatttttgaa aacacggata tctaagaacg gtgtttctag
     3541 ttttaattga cgatgaatga ataaggcgat aaaaataata ccaaccataa taggagaaat
     3601 aacatatcca atatctcccc atccataagt tgccacattt gtgaagcccc acaaaaagct
     3661 accaaagcca attattgaaa ggataaggga aggcacttcc aatttaactg acttattatc
     3721 aaccacatct tttagaacaa agggggataa aattgtagtg accgttaaaa ttagtaaagg
     3781 aagaaggaaa attgcacgcc atgttaaatc atgccctgca aaatgaaatt cttgttttaa
     3841 aatccaccct gctaatgtag gcccaatagc tggggcaaga ccaaccacca aaccattaag
     3901 tcccatagcg gcaccacgct gctctggagg aaaaacattc accataacaa cctgcatcaa
     3961 aggcatagaa atccctacag aaatcgcttg aataatacgt cctactaaaa aaaggttcca
     4021 gttagatgta ggtgctaacg tcgtcattaa cagtccaata agtaaaacaa cgtaggatgt
     4081 cacatacaac cattttgttg aaaaacgtgt cgctaaataa gctgaaacag gaatcataat
     4141 cccattcgct aataaaaacc atgttgttgc ttgttgagcc gttgacaacg aaatgttaaa
     4201 gctattcatc aaagttggga tagccgttcc caaacttgtc tgatttaata cccctgcaaa
     4261 tgtagcaatc aacaataagg tgatcatggc tgtacgatta tatggtttgc catgaatatc
     4321 atgttttgct tttgtagtca ttttatctcc aatctctatt tctgatttta actaatatct
     4381 tttaagaata acaaataggt cttataaggt tattttttga ttttttaatc aagaaataat
     4441 attattttct gaaaaaatca ttattttagt tatcgtgaga actattttat aata
//
LOCUS       DATRUK010000093         4474 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.093, whole genome shotgun sequence.
ACCESSION   DATRUK010000093 DATRUK010000000
VERSION     DATRUK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.093"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..1121
                     /locus_tag="VBE34_001835"
     rRNA            <1..1121
                     /locus_tag="VBE34_001835"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1176..1248
                     /locus_tag="VBE34_001836"
     tRNA            1176..1248
                     /locus_tag="VBE34_001836"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1209..1211,aa:Ala,seq:tgc)
     gene            1403..4305
                     /locus_tag="VBE34_001837"
     rRNA            1403..4305
                     /locus_tag="VBE34_001837"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4380..>4474
                     /gene="rrf"
                     /locus_tag="VBE34_001838"
     rRNA            4380..>4474
                     /gene="rrf"
                     /locus_tag="VBE34_001838"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1251 a          913 c         1295 g         1015 t
ORIGIN      
        1 ggatcgtaaa gctctgttgt tagagaagaa cgttggtagg agtggaaaat ctaccaagtg
       61 acggtaacta accagaaagg gacggctaac tacgtgccag cagccgcggt aatacgtagg
      121 tcccgagcgt tgtccggatt tattgggcgt aaagcgagcg caggcggttc tttaagtctg
      181 aagttaaagg cagtggctta accattgtac gctttggaaa ctggaggact tgagtgcaga
      241 aggggagagt ggaattccat gtgtagcggt gaaatgcgta gatatatgga ggaacaccgg
      301 tggcgaaagc ggctctctgg tctgtaactg acgctgaggc tcgaaagcgt ggggagcaaa
      361 caggattaga taccctggta gtccacgccg taaacgatga gtgctaggtg ttaggccctt
      421 tccggggctt agtgccgcag ctaacgcatt aagcactccg cctggggagt acgaccgcaa
      481 ggttgaaact caaaggaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt
      541 cgaagcaacg cgaagaacct taccaggtct tgacatcctt ctgaccggcc tagagatagg
      601 ctttctcttc ggagcagaag tgacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag
      661 atgttgggtt aagtcccgca acgagcgcaa cccctattgt tagttgccat cattaagttg
      721 ggcactctag cgagactgcc ggtaataaac cggaggaagg tggggatgac gtcaaatcat
      781 catgcccctt atgacctggg ctacacacgt gctacaatgg ttggtacaac gagtcgcaag
      841 ccggtgacgg caagctaatc tcttaaagcc aatctcagtt cggattgtag gctgcaactc
      901 gcctacatga agtcggaatc gctagtaatc gcggatcagc acgccgcggt gaatacgttc
      961 ccgggccttg tacacaccgc ccgtcacacc acgagagttt gtaacacccg aagtcggtga
     1021 ggtaaccttt taggagccag ccgcctaagg tgggatagat gattggggtg aagtcgtaac
     1081 aaggtagccg tatcggaagg tgcggctgga tcacctcctt tctaaggata aggaaacctg
     1141 ccatttgcgt cttgtttagt tttgagaggt cttgtggggc cttagctcag ctgggagagc
     1201 gcctgctttg cacgcaggag gtcagcggtt cgatcccgct aggctccatt gaatcgaaag
     1261 gttcaaattg ttcattgaaa attgaatatc tatatcaaat tccacgatct agaaatagat
     1321 tgtagaaagt aacaagaaaa taaaccgaaa acgctgtgaa tatttaatga gttttctagt
     1381 tttaaagaaa ctagtttaat aaggttaagt taataagggc gcacggtgga tgccttggca
     1441 ctagaagccg aagaaggacg tgactaacga cgaaatgctt tggggagctg taagtaagcg
     1501 ctgatccaga gatgtccgaa tgggggaacc cactagttaa tgactagtat ccatatctgt
     1561 taaggatatg agaaggaaga cgcagtgaac tgaaacatct aagtagctgc aggaagagaa
     1621 agcaaaagcg attgccttag tagcggcgag cgaaacggca ggagggcaaa ccgaagtgtt
     1681 tacacttcgg ggttgtagga ctgcgatata ggattaatca ttatagaaga attacctggg
     1741 aaggtaagcc aaagagagta acagcctcgt atttaaaatg atagattaac cttagcagta
     1801 tcctgagtac ggcgagacac gcgaaatctc gtcggaatct gggaggacca tctcccaacc
     1861 ctaaatactc tctagtgacc gatagtgaac cagtaccgtg agggaaaggt gaaaagcacc
     1921 ccgggagggg agtgaaatag aacctgaaac cgtgtgccta caacaagttc gagcccgtta
     1981 atgggtgaga gcgtgccttt tgtagaatga accggcgagt tacgatatga tgcgaggtta
     2041 agttgaagag acggagccgt agggaaaccg agtcttaata gggcgccata gtatcatgtt
     2101 gtagacccga aaccatgtga cctacccatg agcaggttga aggtgaggta aaactcactg
     2161 gaggaccgaa ccagggcacg ttgaaaagtg cttggatgac ttgtgggtag cggagaaatt
     2221 ccaaacgaac ttggagatag ctggttctct ccgaaatagc tttagggcta gcgtcgatgt
     2281 taagtctctt ggaggtagag cactgtttgg gtgaggggtc catcccggat taccaatctc
     2341 agataaactc cgaatgccaa cgagatataa tcggcagtca gactgcgagt gctaagatcc
     2401 gtagtcgaaa gggaaacagc ccagaccacc agctaaggtc ccaaaatata tgttaagtgg
     2461 aaaaggatgt ggggttgcac agacaactag gatgttagct tagaagcagc tattcattca
     2521 aagagtgcgt aatagctcac tagtcgagtg accctgcgcc gaaaatgtac cggggctaaa
     2581 acatattacc gaagctgtgg atgacacaaa agtgtcatgg taggagagcg ttctatgtgt
     2641 gaagaaggtg taccgtgagg agcgctggaa cgcatagaag tgagaatgcc ggtatgagta
     2701 gcgcaagaca ggtgagaatc ctgtccaccg taagactaag gtttccaggg gaaggctcgt
     2761 ccgccctggg ttagtcggga cctaaggaga gaccgaaagg tgtatccgat ggacaacagg
     2821 ttgatattcc tgtactagag tatatagtga tggagggacg cagtaggcta actaaaccag
     2881 acgattggaa gtgtctggtc aaacagtgag gtgtgatatg agtcaaatgc ttatatcttt
     2941 aacattgagc tgtgacgagg agcgaagttt agtagcgaag ttagtgatgt cacactgcca
     3001 agaaaagctt ctagcgatac atatactcta cccgtaccgc aaaccgacac aggtagtcga
     3061 ggcgagtagc ctcaggtgag cgagagaact ctcgttaagg aactcggcaa aatggccccg
     3121 taacttcggg agaaggggcg ctgattttag atcagccgca gtgaataggc ccaagcaact
     3181 gtttatcaaa aacacagctc tctgctaaat cgtaagatga tgtatagggg gtgacgcctg
     3241 cccggtgctg gaaggttaag aggagtgctt agcgtaagcg aaggtatgaa ttgaagcccc
     3301 agtaaacggc ggccgtaact ataacggtcc taaggtagcg aaattccttg tcgggtaagt
     3361 tccgacccgc acgaaaggcg taatgatttg ggcactgtct caacgagaga ctcggtgaaa
     3421 ttttagtacc tgtgaagatg caggttaccc gcgacaggac ggaaagaccc catggagctt
     3481 tactgcagtt tgatattgag tatctgtacc acatgtacag gataggtagg agcctatgat
     3541 aacgggacgc cagtttcgtt tgaggcgttg ttgggatact acccttgtgt tatggctact
     3601 ctaacccaga taggcaatcc ctatcggaga cagtgtctga cgggcagttt gactggggcg
     3661 gtcgcctcct aaaaggtaac ggaggcgccc aaaggttccc tcagactggt tggaaatcag
     3721 tcgtagagtg taaaggtata agggagcttg actgcgagag ctacaactcg agcagggacg
     3781 aaagtcgggc ttagtgatcc ggtggttccg tatggaaggg ccatcgctca acggataaaa
     3841 gctaccctgg ggataacagg cttatctccc ccaagagttc acatcgacgg ggaggtttgg
     3901 cacctcgatg tcggctcgtc gcatcctggg gctgtagtcg gtcccaaggg ttgggctgtt
     3961 cgcccattaa agcggcacgc gagctgggtt cagaacgtcg tgagacagtt cggtccctat
     4021 ccgtcgcggg cgtaggaaat ttgagaggat ctgctcctag tacgagagga ccagagtgga
     4081 cttaccgctg gtgtaccagt tgtcttgcca aaggcatcgc tgggtagcta tgtagggaag
     4141 ggataagcgc tgaaagcatc taagtgcgaa gcccacctca agatgagatt tcccatgatt
     4201 ttatatcagt aagagccctg agagatgatc aggtagatag gttaggagtg taattgtggt
     4261 gacacattta gcggactaat actaatagct cgaggactta tccaaaaaga gatattgaca
     4321 acgttacggt ttcttgttag actatagata ttcaattttg agtgaataag actcaatagt
     4381 taagtgataa ttgcctagga gatacacctg ttctcatgcc gaacacagaa gttaagccct
     4441 agaacgccgg aagtagttgg gggttgcccc ctgt
//
LOCUS       DATRUK010000094         4463 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.094, whole genome shotgun sequence.
ACCESSION   DATRUK010000094 DATRUK010000000
VERSION     DATRUK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.094"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            157..924
                     /gene="cfb"
                     /locus_tag="VBE34_001839"
     CDS             157..924
                     /gene="cfb"
                     /locus_tag="VBE34_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfb"
                     /protein_id="HEO7264522.1"
                     /translation="MNVKHMMYLSGTLVAGALLFSPAVLEVHADQVTTPQVVNHVNSN
                     NQAQQMAQKLDQDSIQLRNIKDNVQGTDYEKPVNEAITSVEKLKTSLRANPETVYDLN
                     SIGSRVEALTDVIEAITFSTQHLTNKVSQANIDMGFGITKLVIRILDPFASVDSIKAQ
                     VNDVKALEQKVLTYPDLKPTDRATIYTKSKLDKEIWNTRFTRDKKVLNVKEFKVYNTL
                     NKAITHAVGVQLNPNVTVQQVDQEIVTLQAALQTALK"
     gene            1097..1399
                     /locus_tag="VBE34_001840"
     CDS             1097..1399
                     /locus_tag="VBE34_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO7264523.1"
                     /translation="MTKKITVVALETLIAQHNNIHLIDVREEHEYRGGHIPGAINLPL
                     SQLSHKFEQLDKNKEYYLVCQRGGRSIRACQFLELKGYKVINVDGGTEAWRGNLEV"
     gene            complement(1556..2419)
                     /locus_tag="VBE34_001841"
     CDS             complement(1556..2419)
                     /locus_tag="VBE34_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7264524.1"
                     /translation="MNQEKKRLNLIGLILLAQAIVLSLATTLFAEILQNDVWIGIAST
                     LIALLIPCFFMGFKNLKKHLTATHHTITFTTILFLLSIIYTTNNIVSTIWDAVLNHLN
                     IMESESQNYDATFSILMISYSVVVGPFFEEVLYRGIVLNLLSKYGKWFAIITSGILFG
                     LMHQDISQLLTTSIAGIIMGFIAYHYSFKVALLLHICNNFIVEIFTQLSTVNELYGTY
                     FENILLILAILFILYYLFTHRNTARRRISLHSNVREADSINSKQHTKLLLTSWPFILL
                     VIYDIVLTTIN"
     gene            complement(2724..3284)
                     /locus_tag="VBE34_001842"
     CDS             complement(2724..3284)
                     /locus_tag="VBE34_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEO7264525.1"
                     /translation="MRITIFCGASTGENPVYSEKTVALAQWMAQNKHALVYGGGKVGL
                     MGVMADTVIANGGYTTGVIPTFLRDREIAHENLSELIIVNNMPERKAKMMLLGDAFIA
                     LPGGPGTLEEISEVISWSRIGQNDNPCILYNVNGYFNDLKNMFDHMVSEGFLSLEDRE
                     NVLFSDDIAEIEDFITNYKVPSTRQY"
     gene            complement(3281..3598)
                     /locus_tag="VBE34_001843"
     CDS             complement(3281..3598)
                     /locus_tag="VBE34_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEO7264526.1"
                     /translation="MDITDYQKWVSEFYKKRNWYQYNSFIRSNFLSEEVGELAQAIRK
                     YEIGRDRPDETEQTDLENLNDIKEELGDVLDNIFILADQYNISLEEIISAHRTKLEKR
                     FET"
     gene            3812..4426
                     /locus_tag="VBE34_001844"
     CDS             3812..4426
                     /locus_tag="VBE34_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO7264527.1"
                     /translation="MNKKSFFQCVILLVSFLVLTLAVKSQSDMISYLDNITSAFFQSI
                     RNSDLTNLMTIISTVVSPLTTSLIALVILGYQYFLNQRIAVWLFMLFFGTNALALLLK
                     DIIARHRPMNQLIFDSGYSFPSGHTISTFLLMILVLVVARQRLRRVLSQVVFVIFALV
                     ILASVIFSRLYLENHFLTDILGSLLLGASSYCGLSAIVSLKELQ"
BASE COUNT         1524 a          681 c          781 g         1477 t
ORIGIN      
        1 tatgaaaaaa atgcagttta tttgtaataa tgtaaaatat aggggaaaag aaagcgcttg
       61 acgacctttt ggacaagtag taagatacaa acatgggccc tgtaaattaa aaatactgca
      121 gtaaaagtga ttttagttta aaggaggaaa tttattatga acgttaaaca tatgatgtat
      181 ctatctggaa ctctagtggc tggtgcattg ttattttcac cagctgtatt agaagtacat
      241 gctgatcaag tgacaactcc acaagtggta aatcatgtaa atagtaataa tcaagcccag
      301 caaatggctc aaaagcttga tcaagatagc attcagttga gaaatatcaa agataatgtt
      361 cagggaacag attatgaaaa accggttaat gaggctatta ctagcgttga aaaattaaag
      421 acttcattgc gtgccaaccc tgagacagtt tatgatttga attctattgg tagtcgtgta
      481 gaagccttaa cagatgtgat tgaagcaatc actttttcaa ctcaacattt aacaaataag
      541 gttagtcaag caaatattga tatgggattt gggataacta agctagttat tcgcatttta
      601 gatccatttg cttcagttga ttcaattaaa gctcaagtta acgatgtaaa ggcattagaa
      661 caaaaagttt taacttatcc tgatttaaaa ccaactgata gagctaccat ctatacaaaa
      721 tcaaaacttg ataaggaaat ctggaataca cgctttacta gagataaaaa agtacttaac
      781 gtcaaagaat ttaaagttta caatacttta aataaagcaa tcacacatgc tgttggagtt
      841 cagttgaatc caaatgttac ggtacaacaa gttgatcaag agattgtaac attacaagca
      901 gcacttcaaa cagcattaaa ataatatttg tatttttcgt gtgatgctgt cgacttcgtg
      961 attttgtact accatgattg ttatgattaa aagatttacg acaatagtca taatagtaga
     1021 acgatgtcac cattttaaat aataaagtga ttagtcattt gactaaattt gccaagtatc
     1081 aaaggaaata aagattatga ctaaaaagat aactgttgta gcattagaaa cattgattgc
     1141 ccagcataat aatatccatt tgatagacgt tcgtgaagag catgagtatc gtggagggca
     1201 tattccaggt gcgataaatc ttcctttgag tcaactcagt cataagtttg aacagttaga
     1261 taaaaataag gaatattatc ttgtttgtca acgaggggga agatctatta gagcatgcca
     1321 atttctcgag ttgaaaggct acaaagtaat caatgtagat ggtggcacag aagcgtggag
     1381 aggaaatcta gaagtgtaaa tatgtcctgt gtagtctacc atatttgata ataaaaaaat
     1441 tgacattatc tcactattaa tgacctttta gtaaacataa agaaaagtat gtgttttgaa
     1501 aaggaggatg attatccttt tcaaaaccat actttttgtg tcttgtggct tggtcttaat
     1561 taatagttgt taggacaatg tcgtaaatga caaggagaat aaaaggccaa ctagtaagta
     1621 ataattttgt gtgttgttta gagtttattg agtcagcctc tcgaacgtta gagtgcagac
     1681 ttatacgacg gcgagcagta ttcctatgcg taaaaaggta atataatata aataagattg
     1741 ctaaaattag caatatattt tcaaaataag tcccgtataa ctcgtttact gttgatagct
     1801 gggtgaaaat ttctacgata aagttattgc agatatgaag gagtagggct accttaaagg
     1861 aataatggta agcgataaaa cccattatga tacccgcaat agatgtagtc aatagctgag
     1921 aaatgtcttg gtgcattaaa ccaaatagga tgccagaagt gataatggca aaccatttac
     1981 catacttgga taataaattc aatactattc ctcggtataa aacttcttca aaaaaaggac
     2041 ctacaacaac agaataacta atcataagta tactgaatgt ggcatcataa ttttgacttt
     2101 ccgattccat aatattgaga tgatttagca cagcatccca tattgtggag actatattat
     2161 tagtagtata tataatgctt aacaagaata aaatagtggt gaaagtgatt gtatgatgag
     2221 ttgccgttag atgttttttt aaatttttaa aacccataaa aaaacaagga atgagtaagg
     2281 cgattaaagt acttgctata cctatccaga catcattttg taagatttca gcaaatagtg
     2341 tagtcgccaa acttaaaaca atagcctgag ctaaaagtat cagcccgatt aggttaagtc
     2401 tctttttttc ttgattcaat tgattacctc cttattaata acggtaacga caaagaaata
     2461 ttataatgta taatgtatag gtttctaagt cattagtcaa tagggacgag cacttggata
     2521 cttctaataa gataaaatac atagacaaga ctatggtttt ggcttctcta aagattacct
     2581 aaaaatttat aagttctcga gcagaaagga tgagtttgga tattgagagg atgggcttga
     2641 ccagctgaaa gaaaaatgaa ccaagggtaa tcgcttcgaa aaaagaagga aatttcaatt
     2701 agaaacttcc tttttttatc aatctagtat tggcgcgtgc tagggacttt ataattcgtt
     2761 ataaaatctt cgatttcagc aatatcatct gagaatagga cattttctct atcttccaga
     2821 ctaagaaaac cctcactaac catatgatca aacatatttt ttaaatcatt aaagtagcca
     2881 ttaacattat ataaaatgca aggattatca ttttgaccga tgcgagacca agagataact
     2941 tcggaaattt cttcaagggt accaggtcca ccaggtagag cgataaaggc atctccaagt
     3001 agcatcattt ttgccttacg ttcaggcata ttatttacta tgataagctc tgataaattt
     3061 tcatgtgcaa tttcacgatc tcttaaaaat gtcggaataa caccagtagt atagccacca
     3121 tttgcaatca cagtatcagc catgactccc attaagccaa cttttcctcc accatacacc
     3181 agagcatgtt tgttttgtgc catccattgt gccaaggcaa ctgttttttc actataaaca
     3241 ggattttccc cagtacttgc tccacaaaaa atagttattc tcatgtttca aacctctttt
     3301 ctaatttagt gcgatgtgcg ctgataatct cttctagtga gatattatac tgatcagcaa
     3361 gtatgaagat attatctaag acatctccta gttcttcttt tatatcattt aagttttcca
     3421 aatcagtttg ttcggtttcg tcaggtctgt cacgtccaat ttcatatttg cgaatggctt
     3481 gagccaattc gccgacttct tctgaaagaa agttacttct aataaaagaa ttgtattgat
     3541 accagtttct ttttttatag aattcgctca cccatttttg ataatctgta atatccattg
     3601 ttccctccgt ttcaagtata ttgtagcaat attgctttat taatgaaaat ttgaacgcta
     3661 attaatagga aaagttcgta tttggtattt cttctagttt gtgaatagtc atatttaaac
     3721 ttaagaggtt agcgcgtgct ttaagcattt tttaaatatt ttaaaccata atgtatgaag
     3781 atttatctct ttattaaagt gaggttatgt agtgaacaag aaatcctttt tccagtgtgt
     3841 tatactactt gtttcttttt tagtgttaac attagcagtt aaatctcaaa gtgatatgat
     3901 aagttatttg gataatatca cttcagcttt ttttcaaagc atacgaaact ccgatttaac
     3961 taacttaatg acgataatat caacagttgt cagccctttg acaacgtcat taatagcact
     4021 tgttatcctt gggtatcaat attttttaaa ccaaaggata gctgtgtggc tatttatgtt
     4081 attttttggt accaatgctt tggcattgct tttaaaagat atcatcgcac gtcatagacc
     4141 tatgaatcaa cttatttttg actcaggtta tagttttccc agtggacata cgatatcaac
     4201 gtttttatta atgattcttg ttttagtggt tgcaagacag agattgagaa gagtattgtc
     4261 acaagtagtt ttcgtaatat ttgcattagt aatccttgca tcagttattt tttcaagact
     4321 ttatttagaa aatcattttt taacggatat tttagggtcg ttgttactag gagcatcgtc
     4381 atattgtggt ttgtctgcta ttgtatcttt gaaagagtta caataaaaat agtagttacc
     4441 aatgaattat aaaagagcct ttc
//
LOCUS       DATRUK010000095         4346 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.095, whole genome shotgun sequence.
ACCESSION   DATRUK010000095 DATRUK010000000
VERSION     DATRUK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.095"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            102..1639
                     /locus_tag="VBE34_001845"
                     /pseudo
     CDS             102..1639
                     /locus_tag="VBE34_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            1816..3360
                     /locus_tag="VBE34_001846"
     CDS             1816..3360
                     /locus_tag="VBE34_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEO7264528.1"
                     /translation="MTLQDEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLDLLEELEEVLG
                     IASYPMNWPIGMGKSFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VIDPLAKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPELFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWINSDDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     KLEEKM"
     gene            3446..3826
                     /locus_tag="VBE34_001847"
     CDS             3446..3826
                     /locus_tag="VBE34_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEO7264529.1"
                     /translation="MGIFSGLMGNASQVDTDKVENQLSDILIPGEQVDLAYTLIRDLI
                     VFTNYRLILVDKQGVTGKKVSYNSIPYASISRFTVETSGHFDLDAELKIWISSAIEPA
                     EVLQFKNDRNIVSIQKALAIAVLK"
     gene            4020..>4346
                     /locus_tag="VBE34_001848"
     CDS             4020..>4346
                     /locus_tag="VBE34_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7264530.1"
                     /translation="MIIELKSVGKIFPKSQFRLDDISFNIAEGEIVGLIGSNGTGKST
                     ILKMINGLIPYDKGNIYYQGKEVKSFSDNKLRQMRKDIAYIFQNHNLLAGESVYYHLA
                     LVYKLNH"
BASE COUNT         1453 a          642 c          883 g         1368 t
ORIGIN      
        1 tgattgaccg ctaattatta atctgctaag atatagtacg taataaaaac ttaaccggtt
       61 aattattttt tgaaatatat tttttctgga ggaaaatagt aatgaataaa tcattcaata
      121 ccaaattagg tttggtaact gtagcggcat tatcaggtat tgtattaacg tctcaattac
      181 ctgtgaacgc taaagctgag acaccagtaa tggcagcaag tgcacaacaa ggtttccgtt
      241 ttgtagcaac cgttgtagat agtcagacgc gtgtcttgcc gggaaaattg gtaactttgt
      301 cagaggtgac aactggtcag cctaaaatta ttgcaagtgt aaaaacgaat gatgccggac
      361 aagctatttt taataatctc cctataaaac gtaaccttag tgtgtctgtt gatggacaag
      421 taaaaggata tactattcga actgacgtag caggctctag taaagcagct agttttacag
      481 caacaggagt agggacaaat gagccaacgt attcaaaaaa aaccatagac attacagtga
      541 gagaccaaaa tgctgaacct gtttcaggca ggacagttac cttaaaaaca catgctggtc
      601 gtgagattgc tagcttggtt tctggtgaca atggattaac tcgctttact gatcgtttac
      661 tagatggaac attctaccaa tattttgttg atgggaaaaa attggtgata ttgtacctgg
      721 agaatcaaga agtgcatatg ttaatgttgc acctaaaaaa gattatttta cctttacagt
      781 cactgcttta gataaagatg gacttgttgt taagggaaaa gaagtaacgt tgactgatat
      841 tactgatggt aaagcagtag cgcttgcttc tttaaaaacg aatgataatg gacaagctat
      901 ctttaccaac ctaccattat caagaaatat cagtgtttca attgatggaa aatcaaaggg
      961 ctatactctt cgaactgatg tagcaggctc tcataaagcg gctgcattct atgtagatgg
     1021 taaaggtacc aaagcacctc aatttagtgc agaagcagcg gttgtaacta tttacgatgc
     1081 taatggaaat actatagcaa accaagaagt gacattaact aattcaaatg ggacaattgt
     1141 tgcaaaaggt ttgactgaaa aagatggcaa agcaaggttc gccaataaat taatgggagg
     1201 aacgttatac aatatttttg taaatggtat tgagatgcct aaaactgccc ttgttggtag
     1261 tgatgtcagt gtttttctaa cagataaaca aattaagaaa gaaaaacctg ttactcctga
     1321 taaattggat aaagaaatga accaagagaa ggaaaaggag ctcttagata ctccaaaacc
     1381 agtaccacct acttcgaaac caacagcacc aattaaaaat aaaaaggacg attcccctaa
     1441 gaagtttacg aaaagtcaac cttctagttt gaagtcaagt gttaaggcta taggtgctaa
     1501 gccaaaagct atgacagcct tagctaaaaa attgcctaaa actggtgatc aagccattag
     1561 tattttaact ttggttggtt ttattgtaac agggttagcg attatgttag gcttctttaa
     1621 aaaatcacga tcaaaataat ttattttaga agcttgggtc tttagacaca ggcttctctt
     1681 ctttcttcgg aaatagattg aacttgaaga atgttgttaa attcgctata ataataaagc
     1741 tatagacaag attattgttt cagttgagac tactgaaact aatactaaga aatggataaa
     1801 agctaaggat tgaatatgac attacaagac gaaatcaaaa aacgccgtac ttttgccatc
     1861 atctctcacc cggatgctgg taagacgact attactgagc aattattata ttttggtggt
     1921 gaaattagag aagcagggac agtaaaaggg aaaaaatcag gtacttttgc aaagtccgac
     1981 tggatggata ttgaaaagca acggggtatc tctgttactt catctgttat gcaatttgat
     2041 tacgcgggta aacgtgttaa tattttggat acacctggac atgaggattt ctcggaagat
     2101 acataccgga ccttaatggc tgtggatgca gctgtcatgg ttgtagattc ggctaaaggt
     2161 attgaggctc aaactaaaaa actttttgaa gttgttaagc atcgtaatat tccggttttt
     2221 acgtttatta ataaattgga ccgtgatgga cgtgagccac tggatttgct tgaagagtta
     2281 gaagaagtac taggtattgc ttcttatcca atgaattggc caattggtat ggggaaatca
     2341 tttgaagggc tttatgattt acataataaa cgtttagaac tctataaagg agacgagcgt
     2401 tttgcaagta ttgaagacgg tgatcagctg tttgccaata atccatttta tgaacaagtc
     2461 aaagaagata ttgaacttct tcaagaagca ggtaatgact tttcagaaca agctattctt
     2521 gatggtgacc taactccagt attctttggc tctgccctta cgaactttgg agttcagact
     2581 tttcttgata ctttcttgga gtttgctcca gaacctcatg gtcacaagac tacagaaggt
     2641 aatgttattg accccttagc taaagatttt tcaggttttg tctttaaaat tcaagctaac
     2701 atggacccac gtcatcgcga ccgtattgct tttgttcgta ttgtttctgg tgaatttgag
     2761 cgcggcatgg gagttaattt gacacgcact ggtaaaggcg ctaagctctc caatgttact
     2821 caatttatgg cagaatcacg agaaaatgtg acgaatgctg ttgctgggga tattatcggg
     2881 gtttatgata caggaactta tcaagttggg gataccttaa cggttgggaa aaataaattt
     2941 gaatttgaac cattaccaac ttttacccca gaattattta tgaaagtatc agcaaaaaat
     3001 gttatgaagc agaaatcatt ccacaagggt attgagcaat tagtacaaga aggagcaatc
     3061 cagctttata agaattatca gacaggcgaa tacatgctgg gtgctgtggg tcaacttcag
     3121 tttgaagttt ttaaacatcg tatggaaggt gaatacaatg ctgaagttgt tatgacacca
     3181 atgggtaaaa agactgtacg ttggattaac tcagacgatt tagacgaacg catgtcctca
     3241 agccgaaata ttttagcaaa agatcgtttt gaccaaccag ttttcctatt tgaaaatgat
     3301 tttgcacttc gctggtttgc tgataagtat cctgatgtga agcttgaaga aaaaatgtaa
     3361 aatttaaagt cactcaaatt tgagtgactt tttaagtttt ttagactaaa atagttataa
     3421 tacatagaaa agattggaga ttaacatggg aattttttcg ggattaatgg gaaatgcttc
     3481 acaagtggat accgataaag tcgagaatca gttatctgat attctgattc caggtgaaca
     3541 ggttgattta gcttatactt taattcgtga tttgattgtt tttactaatt atcgtttaat
     3601 tttagtcgat aaacaggggg tgacaggtaa aaaggtttct tataattcta taccttatgc
     3661 ttctatatca cgttttacag tggaaactag tggtcatttt gatttagatg ctgagttaaa
     3721 gatttggata tcatcagcaa tagaaccagc agaagtactt cagtttaaga acgaccgtaa
     3781 catagtgagt atacagaaag ctttggcgat agctgtttta aaataaacaa tttagcaagt
     3841 gctgctacta agagttattt gttttaatta taacttttaa tagtgctaaa ttctcatttt
     3901 caaaataaaa gtagtgattt ttatcactgc ttttattttg aagtatagta ggcctcaagc
     3961 atatattttg aaaacgctgt atttttttga taagatattt ttttgaggag tattaaataa
     4021 tgataataga attaaagagt gttgggaaaa tttttccaaa atcacagttt agattagatg
     4081 atatttcttt taatatagca gaaggcgaga tagttggtct gattggttct aatggtactg
     4141 gaaagagtac tattttgaag atgattaatg gcttaatccc ttatgataaa ggaaatattt
     4201 attatcaagg aaaagaagtg aagtcttttt ctgataataa gttacgtcag atgcgtaaag
     4261 atattgctta tatttttcaa aatcataatt tgttagcagg agaaagtgta tattatcatc
     4321 tagctttagt ttataaatta aatcat
//
LOCUS       DATRUK010000096         4176 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.096, whole genome shotgun sequence.
ACCESSION   DATRUK010000096 DATRUK010000000
VERSION     DATRUK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.096"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(344..2320)
                     /gene="ftsH"
                     /locus_tag="VBE34_001849"
     CDS             complement(344..2320)
                     /gene="ftsH"
                     /locus_tag="VBE34_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEO7264531.1"
                     /translation="MKNNKNNGFLKNSFIYILLIIAVITTFQYYLKGTSSQNQQISYT
                     KLVKQLKAGEIKSISYQPSGGVVEVSGTYKKAKTIKSANSFTFLGGSVATKVTGFNSV
                     ILPNDSSIKSLVSAAEENNTNIQVKHESSSGTWISYIASFLPLVIMIGFFMMMMNQGG
                     GGGARGAMSFGKNKARSSSKDEVKVRFSDVAGAEEEKQELIEVVDFLKDPKRYKSLGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDA
                     KKAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNESIIVIAATN
                     RSDVLDPALLRPGRFDRKVLVGQPDVKGREAILRVHAKNKPLADNVDLKVVAQQTPGF
                     VGADLENVLNEAALVAARRNKKVIDASDIDEAEDRVIAGPSKKDRTISERERAMVAYH
                     EAGHTIVGLILSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKDDMKEQLAGLMGG
                     RVAEEIIFNAQTTGASNDFEQATAMARAMVTEYGMSEKLGPVQYEGNHAMMAGQMSPE
                     KSYSAQTAQLIDDEVRHLLNEARNKAADIINENRDTHKLIAEALLKYETLDAAQIKSI
                     FETGKMPETENDEDKARALSYDEIKEKMQEEDKK"
     gene            complement(2343..2885)
                     /gene="hpt"
                     /locus_tag="VBE34_001850"
     CDS             complement(2343..2885)
                     /gene="hpt"
                     /locus_tag="VBE34_001850"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEO7264532.1"
                     /translation="MLENDIKKVLYSEEDIILKTKELGAKLTADYAGKNPLLVGVLKG
                     SVPFMAELLKHIDTHVEIDFMVVSSYHGGTTSSGEVKILKDVDTNIEGRDVIFIEDII
                     DTGRTLKYLRDMFKYRQANSVKVATLFDKPEGRLVDIDADYVCYDIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEIYSK"
     gene            complement(2890..4164)
                     /gene="tilS"
                     /locus_tag="VBE34_001851"
     CDS             complement(2890..4164)
                     /gene="tilS"
                     /locus_tag="VBE34_001851"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEO7264533.1"
                     /translation="MYNTILKDTLSKGLFTAHQKVLIAVSGGIDSINLLQFLYQYQKE
                     LSISIGIAHINHGQRKESEKEEEYIRQWGQIHDVPVFISYFQGIFSEDRARNHRYNFF
                     SKVMREEGYTALVTAHHADDQAETVFMRILRGSRLRYLSGIKQVSAFANGQLIRPFLP
                     YKKELLPNIFHFEDASNASSDYLRNRIRNIYFPTLERENNQLKDSLITLSEETECLFT
                     ALTDLTRSIEVTNCYDFLRQTHSVQEFLLQDYISKFPDLQVSKEQFRVILKLIRTKAN
                     IDYTIKSGYFLHKDYESFHITKIHPKTDSFKVEKRLELHNIQIFSQYLFSYGKFISQA
                     DITIPIYDTSPIILRRRKEGDRIFLGNHTKKIRRLFIDEKITLKDREEAVIGEQNKEL
                     IFVIVAGRTYLRKPSEHDIMKGKLYIENLEKR"
BASE COUNT         1276 a          823 c          602 g         1475 t
ORIGIN      
        1 tagcaaactt attctgcttt gtcaaccact ttttttaagt tgttaactac gcgctactag
       61 aaactgctct ctcgagacaa cttattcatt atactaaata tttctacttc ctgtcaatac
      121 tatttttgca ttttttcttt tatttttaaa aagttaatat tatttatagt aactaacctt
      181 ctatacttgt ttaatggata gcatttttac cgttgtcatg ttcatatttc atcttcttaa
      241 ttcacaaatt taaacttcat cttcataaaa aacacccctc aaattttatc taaatttgaa
      301 gggtgtttga aatttataaa gttctttaaa aatatatgat gacttatttt ttatcttctt
      361 cttgcatttt ttctttgatt tcatcgtatg ataacgctct cgctttatct tcatcatttt
      421 ctgtctcagg cattttacct gtctcgaaaa tcgatttaat ctgagcagca tcaagagtct
      481 catattttaa gagggcttct gcaattaatt tatgagtatc acggttttcg ttgataatat
      541 cagctgcctt attacgtgct tcattaagaa ggtgacgaac ttcatcatca ataagttgtg
      601 cagtttgagc agaatatgat ttttcaggtg acatttggcc agccatcatt gcatggttac
      661 cttcgtattg aactgggcca agtttctcac tcataccata ttcagttacc atagcgcggg
      721 ccatggcagt ggcttgttca aagtcatttg aggcacctgt tgtctgagcg ttgaaaataa
      781 tttcttccgc tacacgtcct cccataagac ctgctaattg ctctttcata tcatcttttg
      841 aaagaagcat ttgatcttct ttaggtaaag caatcatata tccacctgca cgaccacgtg
      901 gtacgatagt aactttatga acaactcgcg catttgaaag aatcaaaccg acaattgtgt
      961 gcccagcttc atggtaagca accatagctc tttctctttc agaaatagta cgatcttttt
     1021 tagaaggacc agcaattaca cggtcttctg cttcatcaat atctgaagca tcaataactt
     1081 ttttatttcg tcgcgcagca actaaagcag cttcattgag aacattctcc aaatcagcac
     1141 caacaaatcc tggggtttgt tgagctacta cttttaagtc aacattatct gctaatggtt
     1201 tatttttagc atgtactctc agaattgctt cacgaccttt tacatcaggt tgtccaacta
     1261 gaacttttct atcaaaacgt cctggacgca gtaatgcggg atctaacacg tcactacggt
     1321 tggtagcagc gataacgata atactttcgt ttccttcaaa accatccatt tcaattaaaa
     1381 gttggttgag cgtttgttca cgttcatcgt ttccgccacc cataccagct ccacgtcgac
     1441 gtccaacagc atcaatttca tcaatgaaaa taatagcacg ttcagctttt ttagcatctt
     1501 caaacaaaga gcgtactcga ctagctccaa caccgacaaa catttcaaca aaatctgaac
     1561 cggaaatact aaagaatgga acccctgctt caccggcaac agcttttgct aatagggttt
     1621 tacctgtccc tggaggtcct tctaatagaa cccctgctgg aattcgggca cctagagatt
     1681 tatatcgttt aggatcttta aggaagtcaa cgacttctat taattcttgc ttttcttcct
     1741 ctgctccagc aacatccgaa aatcgaactt taacctcatc tttactagat gatcttgctt
     1801 tatttttacc aaagctcatg gcaccacgag cgccaccgcc accgccttgg ttcatcatca
     1861 tcataaagaa accaatcatg ataactaaag gtaaaaaact ggcaatataa gaaatccatg
     1921 tcccacttga actttcatgt ttgacctgaa tattagtgtt attttcttca gcagcgctaa
     1981 caagagattt tattgaagag tcatttggta gtataacaga attaaatcct gtaactttag
     2041 tagcaacact accacctaaa aaagtaaaac tattagctga tttaattgtt ttggctttct
     2101 tatatgtccc actaacttca acaacaccac cacttggctg gtatgaaata gacttaattt
     2161 ctccagcttt taattgttta acaagttttg tataagaaat ttgctggttt tgagagctag
     2221 ttccttttaa atagtattga aaggttgtaa taaccgcaat aatcaataat atgtaaataa
     2281 aggaattttt cagaaaacca ttatttttat tatttttcat agtttaagag agtacctttt
     2341 ttctattttg agtaaatttc ttcttttaac acaccgacat aaggtaggtt acgatagttt
     2401 tcagcatagt ctaaaccgaa tcccacaata aattcatttg ggatatcgta acaaacgtaa
     2461 tcagcatcaa tatctaccaa tcgcccttct ggcttatcaa aaagggttgc aactttgaca
     2521 gagttagctt gacgatactt aaacatatct cgaaggtatt ttaatgtacg tccagtatca
     2581 atgatgtctt caataaagat tacatctctt ccttcaatgt tagtatcaac atcttttaaa
     2641 attttaactt ctccactact agttgtacca ccatgatagc ttgaaacaac cataaaatca
     2701 atttcaacat gtgtatcaat atgctttaat agttcagcca taaaaggcac tgaccccttt
     2761 aatacaccta ctaataatgg attcttacct gcataatctg cagttaattt agctcctaat
     2821 tctttcgttt tcagaataat atcttcttct gaatataaaa ctttttttat atcattttca
     2881 agcatgatgt cacctttttt ctaaattttc aatataaagt ttgcccttca ttatatcatg
     2941 ttcagaaggt tttctcaaat atgttctacc tgcaacaatc acaaatatca actctttatt
     3001 ttgctcccca ataacagctt cttctctgtc ttttagagtt attttctcat caataaatag
     3061 acggcgaatt tttttagtgt gatttcctaa aaatatccta tccccttctt ttcttcttct
     3121 taaaataatc ggagaagtat cataaatagg aatagtaatg tctgcttgag aaatgaattt
     3181 tccataggaa aataaatatt gtgagaatat ttgtatatta tgcaattcta atcttttttc
     3241 aaccttaaaa ctatccgtct taggatggat ttttgtaata tgaaaactct cgtaatcctt
     3301 atgaagaaaa tagcccgatt taatagtata gtcaatattg gctttagttc taatcaattt
     3361 taatattact ctgaactgtt ctttactcac ttgtaaatct ggaaatttgc taatgtaatc
     3421 ttgcaataaa aattcttgga cggaatgagt ttgccttaaa aaatcatagc agtttgttac
     3481 ttcgatgctc cttgttaggt ctgttaaagc cgtgaataaa cactctgttt cctcagagag
     3541 agttatgaga gaatctttca attgattatt ttctctttcc aaagtaggaa agtagatgtt
     3601 tcttattcga tttctcaaat aatcagacga agcattgcta gcatcttcaa aatgaaaaat
     3661 atttggtaat aactcctttt tatatggaag aaatggtcta atcaattgac catttgcaaa
     3721 agcactcact tgcttaatcc ctgataaata gcgtagcctg cttcctctta aaattctcat
     3781 aaatacagtt tcagcttgat catcagcatg gtgagctgtt actaaagccg tgtaaccttc
     3841 ttcacgcata acttttgaaa aaaaattgta gcgatggtta cgtgctctat cttctgaaaa
     3901 tattccttga aaataagaga taaagacagg gacatcatgt atctgtcccc actgtctgat
     3961 atattcttct tctttttctg attcttttcg ctgtccatga tttatgtgtg caataccaat
     4021 actaattgat aactcttttt gatactggta taaaaattgc aacaaattta ttgaatcaat
     4081 gccaccagaa acagcaatta gaactttctg atgagcggta aataaccctt tggataaggt
     4141 atctttcaaa atagtgttat acatattact ttaaaa
//
LOCUS       DATRUK010000097         4145 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.097, whole genome shotgun sequence.
ACCESSION   DATRUK010000097 DATRUK010000000
VERSION     DATRUK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.097"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..1546
                     /gene="fbsB"
                     /locus_tag="VBE34_001852"
     CDS             <1..1546
                     /gene="fbsB"
                     /locus_tag="VBE34_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibrinogen-binding surface protein FbsB"
                     /protein_id="HEO7264534.1"
                     /translation="NRMSVALQKFSKNIFEKQKVNKDLIPIAFWIEKSYRTVGTNEIA
                     ASVGIQGGFYQNFHDYYNYSYLLNSLWYEGNVKEVVKDYENTIRQILSKKHEIEKILN
                     QSTSDISIDDDDYEKGNKELLREKLNIILNLSKRDYRVTPYYEVNKLHTGLILLEDVP
                     NLKIAKDKLFSLENSLKEYKGEKVNYEELRFNTEPLTSYLENKEKFLVPNIPYKNKLI
                     LREEDKYSFEDDEEEFGNELLSYNKLKNEVLPVNITTSTILKPFEQKKIVEDFNPYSN
                     LDNLEIKKIRLNGSQKQKVEQEKTKSPTPQKEAVKEQTEQKISGKTQNVEKKSETVAT
                     PQQSSTAQTSVQQPAPVQPVVQESKASQEEINAAHDAISAYKSTVNIANTAGVTTAEM
                     TSLINTQTSNLSDVEKALGNNKVNNGAVNVLREDTSRLENMIWNRAYQAIEEFNVARN
                     TYNNQMKSETVPVDNDIEAILAGSQAKISHLDNRIGARHMDQAFVASLLEVTEMSKSI
                     SSRIKE"
     gene            1613..2158
                     /locus_tag="VBE34_001853"
     CDS             1613..2158
                     /locus_tag="VBE34_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264535.1"
                     /translation="MKKITTLILASSLLLVATTSVKADDNFEVPTRYVKMSEKSKAFY
                     QRLQEKQRKTHTTVKTFNNSEIRHQLPLKQEKARNDIYNLGILISQESKGFIQRIDNA
                     YSLENVSDIVNEAQALYKRNYDLFEKIKSTRDKVQVLLASHQGNTDLKNFYAELDDMY
                     EHVYLNESRVEAINRNIQKYN"
     regulatory      2309..2398
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2554..3120
                     /gene="thiW"
                     /locus_tag="VBE34_001854"
     CDS             2554..3120
                     /gene="thiW"
                     /locus_tag="VBE34_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEO7264536.1"
                     /translation="MTNHKTKKLTVLAIMIALDVFLTPIFRIEGMAFMSSVVNIIAGV
                     IMGPIYALVMALVTAFIRILTQAGVASVAPLAILGAGPGAFLAGWFYRFYAKDWMSWV
                     GEFIGTGIIGALLSAPIMVWFWTWTADGNHEVLAKASAAQSIFLFMPRFIGATIIGGV
                     IALLLLQGLKRVPVFMTIQALFKGEKVK"
     gene            3117..3671
                     /locus_tag="VBE34_001855"
     CDS             3117..3671
                     /locus_tag="VBE34_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7264537.1"
                     /translation="MKLGLKDVLLIALLAVVLGVVYFGAGYISNAFVPFVGPIAHEVI
                     YGIWFVAGPMALYILRKPGTAIVAELLAALIEVLIGSIYGPSVLVIGTLQGLGSELGF
                     TLFRYHNYKLPAFILSAILTSIFSFAWSFYANGLSAFSFSYNILMLIVRTVSSIIFFL
                     LTKNICDQLHRSGVLNAYGIHEGK"
     gene            3675..>4145
                     /locus_tag="VBE34_001856"
     CDS             3675..>4145
                     /locus_tag="VBE34_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7264538.1"
                     /translation="MLSVEKLAYTHGDSHYLFDEVTFSLNPGERILISGYSGCGKSTL
                     ALLLSGLKESGKGQVLLNGSLIEPSDVGFLFQNPDLQFCMDTVAHELYFILENLQIEP
                     EQMQDRSEFVLAQVGLKGFQNRLIYTLSQGEKQRLALATIFLKSPKLIILDEAFA"
BASE COUNT         1411 a          585 c          802 g         1347 t
ORIGIN      
        1 gaatagaatg agtgttgctt tacaaaaatt ttcaaagaat atttttgaga aacagaaagt
       61 aaataaagat ttaatcccta ttgcgttttg gattgagaaa agttacagaa ctgttggaac
      121 gaatgaaatc gccgcttctg taggcattca aggaggattt tatcaaaact tccatgatta
      181 ttataattat tcatatctat taaattcttt atggtatgaa ggaaatgtaa aagaagtagt
      241 taaggattat gaaaacacta ttcgtcaaat actatctaaa aagcatgaga ttgaaaaaat
      301 tcttaatcag agcacttctg atatctctat agatgatgat gattacgaaa aaggaaataa
      361 agaattgcta agggaaaaat taaatattat tctaaatctt tcaaagagag attacagagt
      421 aactccatac tatgaagtga ataaactaca tacagggctt attttattgg aggatgtccc
      481 taatttaaag attgctaagg ataagttgtt ttcattagag aattctttaa aggaatacaa
      541 aggagagaaa gttaattatg aggaactaag attcaatacg gaacctttaa ctagttactt
      601 agaaaataaa gaaaaatttt tagtccccaa tattccatat aaaaataaat taattttaag
      661 ggaagaagat aaatatagtt ttgaagatga tgaagaagag tttggaaatg aacttctaag
      721 ttacaataag cttaagaatg aagttttacc tgttaatatt acaacttcta ctatattaaa
      781 accgtttgaa cagaagaaaa ttgtggaaga ttttaatcct tattctaatt tagacaattt
      841 agaaataaaa aaaataaggt tgaatggctc ccaaaaacaa aaagtagaac aggaaaaaac
      901 taaatcgcca actcctcaaa aagaggctgt aaaagaacaa actgagcaaa aaatatctgg
      961 gaagactcaa aacgtagaaa agaaatctga aactgtggca actccacaac aaagttcaac
     1021 tgcgcaaact tctgtccaac agccagctcc ggttcaacca gttgttcaag aatctaaagc
     1081 ttctcaagag gagattaatg cagcacatga tgccatttca gcgtataaat caacagtcaa
     1141 tattgctaat acagccggtg taacaactgc ggaaatgacc tcgctcatta atactcaaac
     1201 ttctaatctt tctgatgttg agaaagcttt agggaataat aaggttaata atggtgcagt
     1261 caatgtattg agagaagata catctcgtct tgagaatatg atttggaatc gtgcttacca
     1321 agctattgaa gaattcaacg tcgctcgtaa tacttataat aaccaaatga agtcagaaac
     1381 agttccagtt gataatgata ttgaagctat tttagcaggt tctcaagcta aaattagcca
     1441 tttggacaat cgtatcggag cgcgccacat ggatcaagct tttgtagcta gtttattaga
     1501 agttactgag atgagtaaat caatctcatc gcgtataaaa gaatagacac tgctatcaag
     1561 gcgatcttaa acttttgtat tgagctaacc taaaagatag aaagagacta atatgaaaaa
     1621 aataacaact ttaatcttag ctagtagctt attactagtt gcaacgacat cggttaaagc
     1681 tgatgataac tttgaagtac caacgcgtta tgttaaaatg agtgaaaaat caaaagcatt
     1741 ttatcaaaga ttacaagaaa aacaacgtaa aacacatact actgtgaaga cttttaataa
     1801 ttcagaaata aggcatcaac tacctcttaa acaagaaaag gctaggaatg atatctacaa
     1861 tttaggcatt cttatttctc aggagtctaa agggttcatc caacgtattg ataatgccta
     1921 ttctttggaa aatgtctcag atattgttaa tgaagctcag gctttgtata aacgcaacta
     1981 tgatttattt gaaaaaatca aatctacacg tgataaggtt caagtcttac ttgcatcgca
     2041 tcaaggtaat acagacttaa aaaactttta tgctgagttg gacgatatgt atgaacatgt
     2101 ttatctcaac gaaagtagag tggaggcgat aaacagaaat atccaaaaat ataattagtt
     2161 tctaaactaa caaacattcc taaatataag acattaaacc ctacttattg attagtgagt
     2221 agggttttac tgttttaaat agctttctgc tcagaatgta agccttgtca tttcaaagga
     2281 actatgttat tattcttaag taaattaaat aggacatttg gggtgcgtaa cagctgagat
     2341 tatacccatt gaacttgata cagttagtac tgtcgaaggg aaatgtgtgc cattacaagt
     2401 tttttgtaat ggaaaagggc gtagtgtctt cttaggagac agctagctct agttaatcag
     2461 ataataaact ccgtttgctt gcagatggag ttttttgtcg tcaaaatttt ttataggttg
     2521 ttgcccctta tctgtcatag agaaggagtt atgatgacta atcataaaac aaaaaaatta
     2581 acggttttag caattatgat tgctttagat gtttttctaa cgcccatttt tagaatagaa
     2641 ggcatggcct ttatgtctag tgtggttaat attatcgctg gagttataat gggaccaatc
     2701 tatgcattag ttatggcact tgtaacggct tttattcgta ttttaactca agcaggagta
     2761 gcatctgttg cacctttagc tattctagga gctggtccag gagctttctt agcgggttgg
     2821 ttttatcggt tttatgcgaa agactggatg tcttgggtgg gtgagtttat aggaacaggg
     2881 ataattggag cacttttatc agctccaatt atggtctggt tttggacttg gacagcggat
     2941 ggaaatcacg aagtgttagc taaagcatct gcagctcaat ccatttttct ttttatgcca
     3001 aggtttatag gggcgactat aattggcggt gttattgctt tgttgctact tcagggatta
     3061 aagcgggttc ctgtatttat gacaatacaa gcattattta aaggagaaaa agtaaaatga
     3121 aattgggttt aaaagatgta ttattgattg ctttattagc ggttgttctg ggcgttgttt
     3181 actttggagc aggctatatt tcaaatgctt ttgtaccttt tgtaggtcct atagcgcatg
     3241 aagttattta tggtatttgg tttgtggcag gtccaatggc tctttatata ttgaggaaac
     3301 caggaaccgc tattgtagca gaattgttag ctgctttgat agaggtttta attggatcaa
     3361 tttatggacc aagtgtactc gttattggta cccttcaagg tttagggtca gaattaggct
     3421 ttacgctgtt taggtatcat aattacaagt tgccagcttt tatattgtct gctattttga
     3481 catcaatttt tagttttgca tggagttttt atgccaatgg acttagtgca ttttcatttt
     3541 catataacat tctgatgctt atagtaagaa ctgtttcatc tattattttc ttcttattaa
     3601 caaagaatat atgtgaccaa ttgcatagaa gtggtgtact gaatgcttat gggattcatg
     3661 aagggaagta gcctatgcta tctgttgaaa aattagctta cacccatggc gatagtcatt
     3721 atttatttga tgaagtgact tttagtctta acccaggaga acgaatttta attagtggct
     3781 attcagggtg tggtaagagt acattggctc ttttactatc agggttaaaa gaaagtggga
     3841 aaggtcaagt tttattaaac ggctcattaa tagaaccaag cgatgtgggt tttctttttc
     3901 aaaatcctga tttgcaattt tgtatggata ctgtggcgca tgagttgtat tttattttag
     3961 aaaatttaca aatagaaccg gagcaaatgc aagatagaag tgagtttgta ttggcacaag
     4021 ttggtttaaa aggatttcag aatcgtttga tttatacttt atctcaaggt gaaaaacagc
     4081 gattagcatt ggcgactatt tttttaaaat ctcctaaact tattatttta gatgaagctt
     4141 ttgcg
//
LOCUS       DATRUK010000098         3986 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.098, whole genome shotgun sequence.
ACCESSION   DATRUK010000098 DATRUK010000000
VERSION     DATRUK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.098"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..1554
                     /locus_tag="VBE34_001857"
     CDS             <1..1554
                     /locus_tag="VBE34_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEO7264539.1"
                     /translation="SDLSIIPQTLNHIADQSSVANTRVLTIGVSGLIGGPIVGIIVGL
                     LSVFVRYLQGGLAPHIYVISSLLIGLCSGLSGNYLRKNYNKIRVLDAMVVGFGMEILQ
                     MICILIFSVDFNQALRLVSFISMPMILSNTLGLGIFISIISSTQKLEEHAKAFQTHQV
                     LELANLTLPYLRKGLTTESCQPVAEIIHKHMDVSAVSLTSQSAILAYVGDGADHHLPN
                     TQILTKLAKRAIDTGKVSVATDKSEIECDHRNCPLSSAIVIPLHIHDVIVGTLKLYFS
                     DAQHMTYVDRQLAEGLGNIFSTQLALGQAEEATRLLQDAEMKSLQAQVNPHFLFNALN
                     TIYGLIRMDSEKARKLVQDFSKVIRANLQRAKQNLIPLHDELEQVNAYLALEEARFPN
                     MVAFNLDNQTNSDDNLMIPPFTLQVLIENSYKHAFKHINKNNQLKVTIARNNDRLHII
                     VQDNGIGIPKEKLITLGKKTQISKKGSGTAIENLVRRLNIIYDGQASLKFESNDSGTC
                     AIVNIPIKN"
     gene            1566..2300
                     /gene="ltdR"
                     /locus_tag="VBE34_001858"
     CDS             1566..2300
                     /gene="ltdR"
                     /locus_tag="VBE34_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LtdR"
                     /protein_id="HEO7264540.1"
                     /translation="MKVLVVDDEPVARNELIYLLNKYDSNLVIAEAHDMATALAILLR
                     ETFDVALLDIHLRDDSGLQLAEYINKMPKPPLLIFATAYDQYAIQAFEQDARDYLLKP
                     YEFDRLKQAMDRVKGALSTSTIIESVASGPLFKQQYPLTVEDRIYLVSADDILLIEAM
                     QGKLIIQTPDKNYEIDGSLQQWQDKLPSSQFVRVHRSYIVNINAIKTIEPWFNQTLQL
                     HLCNKITVPVSRANVKPLKQMLGIST"
     gene            2456..3940
                     /locus_tag="VBE34_001859"
     CDS             2456..3940
                     /locus_tag="VBE34_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEO7264541.1"
                     /translation="MVTFLGGVALLIVGYFTYGRYIEKNFQIDENRQTPAEALRDGYD
                     FVPMPKWKNGMIELLNIAGTGPIFGPILGALYGPVAYIWIVLGCIFAGAVHDYMIGMI
                     SLRNNGAYLPELASRYLGKSMKHVINIFSMLLLILVATVFVVTPANLILSILPAGTLS
                     LPWIIGLIFVYYLISTVLPIDKALGKVYPVFCVILMVSTAAVGFRLLTGGFDMPNLTF
                     ETFKNMHPAGLGIFPALFFTISCGAISGFHATQAPMVSRTTVNEREGRFTFYGMMIAE
                     GVIAMIWAGASMSLFKGQNLYEMIAAGTPSAVVNQVMLMLLGSVIGTIAIIGVIVLPV
                     SSGLSAFRSLRTIVADYIHVKQDTLPKIFAVTIPLYVISFVLTHVDFNLLWRYFNWAN
                     QVTAVIGLLVATRYLILKRRNYWVTFVPAMFMLYAVVVYILSQPIGFNMGLGILTYSL
                     ALVLTGILVGLFWKSGQKQLKTVHPEAFLFNDHRPINYYSSLDS"
BASE COUNT         1243 a          697 c          709 g         1337 t
ORIGIN      
        1 tcagatttat ctatcattcc acaaactctt aatcatatag cagatcaatc atcagttgcc
       61 aacacacgtg ttttaaccat tggtgtatca ggtctaatcg gtggccccat agtcggtatt
      121 atagttggtc tcttatctgt attcgttagg tacttgcaag gaggattagc ccctcatatt
      181 tatgtcattt cttccttact aattggttta tgctctgggt tatcaggcaa ttatcttcgt
      241 aaaaattata ataaaattcg tgttttagat gcaatggttg ttggtttcgg gatggaaatt
      301 ctacaaatga tctgtatcct catttttagt gttgacttta atcaggcgct tcgattagta
      361 tcttttatca gcatgccaat gattttaagt aatactttag gtttaggtat atttatatcc
      421 attatttcat ctactcaaaa attagaagaa catgccaaag catttcaaac ccatcaagta
      481 cttgaattag caaatttaac ccttccttac cttagaaagg gactgacaac agaatcttgt
      541 caaccagtag ctgaaattat tcataaacat atggatgtga gtgctgttag cctgactagt
      601 caatcagcca ttttagccta tgtaggagat ggagctgacc accatcttcc taatactcaa
      661 atattaacaa agcttgctaa aagagcaata gatactggta aagttagtgt cgcaactgat
      721 aaaagtgaaa tcgaatgcga ccataggaat tgcccactta gttcagcaat tgttattcca
      781 ttacacatcc acgatgttat agtgggtact ttgaaattat atttttcaga cgcacaacat
      841 atgacttacg ttgaccgtca attagctgaa ggattaggca atatcttttc cactcagtta
      901 gctttgggac aagctgagga agcaacaagg ttactacagg atgcggaaat gaagtctctc
      961 caagcgcagg ttaatccgca ttttcttttt aatgcactca atactattta tggtttaatt
     1021 cgcatggata gcgaaaaagc aagaaagctc gtccaagatt ttagcaaggt tattcgtgct
     1081 aatcttcaac gggcaaagca aaatttaatt cctctccatg atgaattgga acaggttaac
     1141 gcctatttag cccttgaaga agcaagattt cctaacatgg tggcctttaa tttagacaac
     1201 caaacaaatt cagatgacaa cttaatgatt cctcccttta cattgcaggt cttgattgaa
     1261 aactcttata aacacgcttt taagcatatc aataaaaata accagctaaa ggttactatt
     1321 gcaagaaata atgataggct tcatatcatt gtccaggaca atggtattgg gattccaaaa
     1381 gaaaaattaa taacgcttgg aaaaaaaact caaatttcaa aaaaaggttc tggtacagct
     1441 attgagaatc tcgttcgtcg cttgaatatt atttatgatg gacaagcaag tttaaaattt
     1501 gaatcaaatg attcaggaac ttgtgctatt gttaacatcc ccattaaaaa ttagaaaggt
     1561 attgtatgaa agttttagta gttgatgatg aaccagttgc acgtaacgaa ttaatttacc
     1621 ttcttaataa gtatgattct aacctcgtta tagcagaggc gcatgatatg gctactgcat
     1681 tagctatttt acttagagaa acttttgatg tagcactgtt agatatccat ctcagagatg
     1741 attctgggtt gcaattagca gagtatatca ataaaatgcc caaaccacca ttattgatat
     1801 tcgcgactgc ttatgatcaa tatgctattc aggcttttga gcaggatgcg cgtgattatt
     1861 tgttaaaacc ctatgagttt gataggttaa agcaagctat ggatagagta aaaggagcgc
     1921 taagtacatc tacaattata gagagcgtag cttccggtcc tctcttcaag caacagtatc
     1981 cattgacagt agaagatcga atctatctgg tgtcggcgga tgatatcctt ttgattgaag
     2041 ctatgcaagg aaaactgatt atacaaacac ctgataaaaa ttatgaaatt gatggctctc
     2101 tacaacaatg gcaagataaa ctaccatcat ctcaatttgt acgggtacat cgctcttaca
     2161 ttgtgaatat taatgctatt aaaacgattg aaccttggtt taaccaaaca cttcagttac
     2221 acctttgtaa taaaataaca gttcctgtta gcagagcaaa tgtaaaaccc ctaaaacaaa
     2281 tgttaggcat atctacctaa ctaatgaaaa gttattcatc ataaaactta gactattcat
     2341 ttctaaaaaa caaccattca tcttgaaaat aggaaatttc aatttttcaa tgctatacta
     2401 aagtcatgaa aacgcttaat aaaattaagc aaaaaaattt ttaggaggca gtgatatggt
     2461 aacattttta ggaggggtag cccttttaat tgttggttat ttcacgtacg gtagatacat
     2521 tgaaaagaat tttcaaattg atgaaaatcg ccaaacacct gcagaagcat taagggatgg
     2581 atatgatttt gttcctatgc caaaatggaa gaatgggatg atcgagttat tgaacatcgc
     2641 aggaactgga cctatctttg gacctatttt aggagcactc tatggtccag ttgcttatat
     2701 ctggatagtc ttaggatgta tttttgcagg ggctgttcat gattatatga ttggaatgat
     2761 ttctcttaga aataatggtg catatcttcc tgaattagct agtcgctatc ttggaaaatc
     2821 aatgaagcat gtgatcaaca ttttttcaat gctattactt attttagttg caaccgtatt
     2881 tgtggtaact ccagctaatc ttatcctgag tattttacca gctgggacac tatctttacc
     2941 ttggataatc ggtcttattt tcgtttatta ccttatttct acagtattac ctattgataa
     3001 agcacttggt aaagtttatc ctgtattctg cgtcatccta atggttagta cagcagcagt
     3061 tggcttccgt ttattaactg gtggctttga tatgccaaac ttaacttttg aaacctttaa
     3121 aaatatgcat ccagctggac ttgggatatt ccctgcttta ttctttacca tttcatgtgg
     3181 agccatctct ggtttccatg ctacccaagc acctatggtc tcaagaacaa ctgttaatga
     3241 gagagaaggg cgtttcactt tctatggtat gatgattgca gaaggagtaa ttgcgatgat
     3301 ttgggcaggt gcttcaatga gcttgttcaa aggtcaaaat ctctatgaaa tgattgctgc
     3361 tggaacacct tctgcagttg tcaatcaagt aatgttaatg ttactcggta gtgtaattgg
     3421 aactatcgct atcattggtg ttattgttct ccctgtttca tctggtttat cagctttccg
     3481 tagcttacgt actatcgttg ctgattatat ccatgtcaaa caagatactc ttccaaaaat
     3541 ctttgctgta actatcccat tatatgttat ttcatttgtc cttacacacg ttgattttaa
     3601 tctcctatgg cgttacttta actgggcaaa tcaagtaact gctgttattg gtttattagt
     3661 tgctacaaga tatctcatcc taaaaagacg aaattattgg gtaacatttg taccagctat
     3721 gttcatgttg tatgcagtag ttgtttatat tttatctcaa ccaattggat ttaatatggg
     3781 cttaggcatc ttaacttatt cacttgcttt agtgttaaca ggtattcttg ttggactttt
     3841 ctggaaatct ggtcaaaaac aattaaaaac tgttcatcct gaagcattcc tgtttaacga
     3901 tcatagacca attaattatt acagtagttt agattcttaa tattattttg aagccatgtt
     3961 attaagactt acagttatca caataa
//
LOCUS       DATRUK010000099         3893 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.099, whole genome shotgun sequence.
ACCESSION   DATRUK010000099 DATRUK010000000
VERSION     DATRUK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.099"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(86..601)
                     /gene="lacB"
                     /locus_tag="VBE34_001860"
     CDS             complement(86..601)
                     /gene="lacB"
                     /locus_tag="VBE34_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEO7264542.1"
                     /translation="MKIAVGCDHIVTYDKIAVVDYLKTKGYEVIDCGTYDNIRTHYPI
                     YGKKVGEAVASGKADLGVCICGTGVGINNAVNKVPGIRSALVRDLTSAIYAKEELNAN
                     VIGFGGKITGGLLMTDIIEAFIRAKYKPTKENKVLIEKIAEVETHNAHQEENDFFTEF
                     LDKWNRGEYHD"
     gene            complement(622..1046)
                     /gene="lacA"
                     /locus_tag="VBE34_001861"
                     /pseudo
     CDS             complement(622..1046)
                     /gene="lacA"
                     /locus_tag="VBE34_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
     gene            complement(1295..3745)
                     /locus_tag="VBE34_001862"
     CDS             complement(1295..3745)
                     /locus_tag="VBE34_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEO7264543.1"
                     /translation="MTLTFGKPSITIRRLGVITSTYMMLGFILLCGLLHPSTIYSEEI
                     KSDINSFISIDDEKTTAPIFDSTLDKLTNSTEERQLELDSDTSDGLIVDDQKDKDMVV
                     VLGDSDSQEKLSPPLKSEIATEENRKQANLAELNATQPNNRTTYIIPESSHSIAEQQR
                     FLIESKGSSVALLNSDEFRKTAGEDRGFERDKLRSLDIIPKGDLSTSNVIGNTDIASQ
                     ISLGFKKNAMQEHHLTKTFSQKDGKLSSVIEGMLAIGKEKVEKEIKYSGNLWQKLKAK
                     AHCLVCCVDNLNFEDIKSYFQYYCHLNHQLKLPKGAILSAKTEVYRGGDFGRKNKDNV
                     FGYRIPSLLKTQKGTLLAGADERIEQACDWGNIGMVIRRSEDDGVTWGKRETIVNLRN
                     NPRVPLVTSGDYSGSPINMDMALVQDTSSKTKRIFSIYDMFPEGRGVISIANTPEKEY
                     TQIGGQSYLNLYNNGKKSKVFTIRDKGIVYNFKGKKTDYHVITETTKSDHSNLGDIYK
                     GKQLLGNIYFTKHKTSPFRLAKSSYVWMSYSDDDGRTWSSPRDITASLRQKGMKFLGI
                     GPGKGIVLKWGPHAGRIIIPAYSTNWKSHLRGSQSSRLIYSDDHGKTWHTGKAVNDNR
                     ILSNGEKIHSLTMDNKKEQNTESVPVQLKNGDIKLFMRNLTGNLEVATSKDGGETWQN
                     HVKRYKEIHDAYVQLSAIRFEHDKKEYILLVNANEPGKKCQDGYARLAQVNRNGSFKW
                     LYHHHIQDGSFAYNSVQQLNNDQFGVLYEHREKHQNSFTLNYKVFNWSFLSQNTEKQG
                     TLWEKMAANWHVLFKFYL"
BASE COUNT         1152 a          793 c          553 g         1395 t
ORIGIN      
        1 tacagtatcc atgttaaagt tttctaagca gtacgaaata tcaatagaag ggttcaaagt
       61 tactgttaaa atcatagata tctccttagt cgtgatattc accacgattc catttatcta
      121 agaactctgt gaaaaagtca ttctcttctt gatgggcatt atgagtctca acctcggcta
      181 tcttttcaat taatactttg ttttcttttg ttggtttata tttagctctt ataaacgcct
      241 cgatgatatc tgtcattaac agcccaccag taattttccc accaaatccg ataacgttag
      301 catttaattc ttctttagca tagatagctg atgtcaaatc acgtaccaga gctgagcgaa
      361 ttcctggaac tttattaaca gcattattaa taccaactcc agttccacaa atacatacac
      421 ctaagtcggc ttttccacta gcgacagctt ctcctacttt cttaccataa ataggatagt
      481 gagtacggat gttatcataa gttccacaat caataacctc ataccctttt gtttttaaat
      541 aatccacaac tgcaattttg tcataagtta caatatgatc acaaccgacc gcaattttca
      601 tctgctttac ctctcttacg cttagcacat tttattcaac atatcgaccc gaatttgatg
      661 acgacctgca tcgtaagtcc catcaacaaa accctctact atatgtttag caaccccgga
      721 ccaacaatct ctgaacctaa agtaatcatc ctagcgttat tatgagctct agtcatataa
      781 gctgagcgtt catctgaaac ttctgctgca atcatcccct taaccttagt ggctaccata
      841 aaaggaccag caccgtaagc atcaaccatt attcctaagt tatcttcttt ttgattcact
      901 ttagcaacga ttgctaaggt attatcaacg aaatctttgc tagtatccgt taaatcaata
      961 atttccttac ctaaacgtgt aaggtgttgt ctaatcactt ccttaagttc aacaccatgc
     1021 gcatccgccc caataataat tgtcatttct ttctccttat ttcactttta atacaattgt
     1081 atgtagttgg aagatattgt ttgttttttg tttgtaatat catcatatat cttatttttg
     1141 tttttgtcaa tgttttgttt ttgtttttgt ttgaaataaa caaaaaaact ccgtgataac
     1201 tatggcaact agaattcact actagctagc tatcacgaag tttgatactt ctcattattt
     1261 ttatttgtag gaacggttat tccgcaagcc ataatcataa ataaaattta aacaaaacat
     1321 gccaatttgc tgccattttc tcccataaag tgccttgctt ctctgtattt tgactaagaa
     1381 aactccaatt aaaaactttg taatttaaag taaaactatt ttgatgtttt tctctatgtt
     1441 cataaaggac accaaattga tcattattaa gttgttgaac agagttgtaa gcaaacgaac
     1501 catcttgaat gtgatggtga tataaccact taaaactacc atttcgatta acttgcgcta
     1561 gacgtgcata tccatcttgg cacttcttcc ctggctcatt agcattcact aataaaatat
     1621 actctttttt gtcatgctca aagcgaatag ctgatagttg gacgtaagca tcatgaattt
     1681 ccttatatcg tttaacatgg ttttgccaag tctccccgcc gtctttactt gtggctactt
     1741 ctaggttacc agttagattc ctcataaata acttaatgtc cccatttttc aattgaacgg
     1801 gtacggattc tgtattttgt tcttttttat tatccattgt taaggagtga attttttcac
     1861 cattagaaag tatacggtta tcattaactg cttttccagt atgccacgtt tttccatggt
     1921 cgtctgaata aattaggcgt gaagattgtg aacctcttag atgagatttc caattcgtag
     1981 aataggcagg aataataata cgaccagcgt gtggccccca ttttaaaact ataccttttc
     2041 caggtcctat tcccaaaaat ttcatgcctt tctgacgaag acttgctgtt atatctctag
     2101 gtgatgacca tgtcctacca tcatcatcgc tatatgacat ccacacatag cttgattttg
     2161 ctaaacgaaa tggtgacgtt ttatgttttg taaaatatat atttccaagt agctgttttc
     2221 ccttataaat atcccctaga tttgaatggt cacttttagt agtttctgtt ataacatgat
     2281 aatcagtctt tttcccttta aaattatata caataccttt gtcacggata gtaaaaacct
     2341 tcgatttctt tccattatta tataaattaa gataagactg tcctccgatt tgggtatatt
     2401 ctttttcagg tgtgttagca atactaataa cgcctcttcc ttctggaaac atatcatata
     2461 ttgaaaaaat acgtttcgtc ttggagctag tatcttgaac taatgccata tccatattaa
     2521 taggtgagcc actatagtca ccactagtaa ctagcggaac tctagggtta ttacggagat
     2581 tgacaatagt ttctcttttt ccccaagtga caccatcatc ctcactacgg cgaataacca
     2641 ttcctatgtt tccccaatca caagcttgct caattctttc atcagctccc gcaagtaaag
     2701 ttcctttttg ggttttcaat aatgagggga tacggtaacc aaacacatta tctttatttt
     2761 ttctcccaaa atctcctccc ctatatactt ctgttttagc agaaagtata gcacctttag
     2821 gtaatttgag ctgatggttt agatgacaat aatattgaaa ataagatttt atatcttcaa
     2881 aattcaaatt atcaacacag caaacaaggc agtgtgcctt agcttttaat ttttgccata
     2941 aattaccact atattttatt tctttctcta ctttctcttt gccaatagca agcatcccct
     3001 ctataacaga cgataacttt ccatcctttt gagagaatgt tttagtaaga tggtgttcct
     3061 gcatcgcatt ctttttaaag cccaacgata tctgactagc aatgtccgta ttacctatga
     3121 cattacttgt cgataaatct cccttaggaa tgatatccaa agacctcaac ttatcccttt
     3181 caaaacctct atcctctccc gctgtctttc taaattcatc gctattaagt aatgcaaccg
     3241 aagaaccctt tgattctatc aggaatctct gttgttctgc aatggaatga ctgctttcgg
     3301 gtataatata ggtagttcta ttattaggtt gagttgcatt tagttctgct aaatttgcct
     3361 gtttacgatt ttcttctgtc gctatttccg attttagagg aggtgacaat ttttcttgac
     3421 tgtcagaatc acctaagact actaccatat ctttatcttt ttgatcatct actattaaac
     3481 cgtccgatgt atcagaatct aattctaact gcctctcctc tgttgaatta gtaagcttat
     3541 ctaaagtaga gtcaaatatg ggagcagtag ttttttcatc atctattgat ataaaagagt
     3601 ttatatcaga ttttatttcc tcactataaa ttgtactagg atgcaaaagt ccacataata
     3661 agataaaacc tagcatcata tatgtactag ttatcactcc tagacggcgt atcgttatag
     3721 aaggtttccc aaaggttaat gtcatagaaa ctccaagtct taaaatattg gttgcataca
     3781 tcaactctag aaaaatggga gttcagtaat gtttccaaaa aggattgtat accaacttat
     3841 cactattttc aatatatttt tataatttta ctagtttttt attattaaaa tga
//
LOCUS       DATRUK010000100         3872 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.100, whole genome shotgun sequence.
ACCESSION   DATRUK010000100 DATRUK010000000
VERSION     DATRUK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.100"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            15..653
                     /locus_tag="VBE34_001863"
     CDS             15..653
                     /locus_tag="VBE34_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEO7264544.1"
                     /translation="MFLIDFILHIDTHIYAMANTVGNWTYLLLFLVIFVETGAVIFPF
                     LPGDSLLFAAGALAANPKMSFNIVTFLIIFFIAAFIGDSCNFLIGRTFGYRFIKHPFF
                     RRFIKEKNIRDAELYFEKKGTAAIILGRYIPIIRTFVPFVASISQLPPKVFIKRAFIA
                     ALSWSVIATGSGFLFGNIPFVKQHFSLIILGIVFVTLIPVLISGVKSYRNTK"
     gene            complement(731..1517)
                     /locus_tag="VBE34_001864"
                     /pseudo
     CDS             complement(731..1517)
                     /locus_tag="VBE34_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825435.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
     gene            1778..3223
                     /locus_tag="VBE34_001865"
     CDS             1778..3223
                     /locus_tag="VBE34_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEO7264545.1"
                     /translation="MKKTIGIIGMGVSGLAVLLALSRKKEKELKQLAIFCFDDTVHFG
                     RGIPFQEDVDSALINSPINDISFDYRHMSDFMDWLEENYYDTNLTYVSRTLYGQYLSE
                     RTKLLLDKLPVTVIKTRVDKLNYLSDEQQWQVYIQDKLFPVTFDELHLACGALPVIEP
                     YHLEGKTDYIKDPYPIKYLAQENWKGKTVAIIGTGLAAIDVIKWLLLNTQSSVKAFSR
                     TNYFPSVRIVNDLPLTWHFFTNDNLKAFLSHPSKTFTIADFNDLMSGELEHLGFKNWK
                     NTTETFLSPGIDGLKLAFKYPKQLYLLQQLASRITVWLTDLWPLMSLSDKKEYKALYG
                     KAIINLRNPMPDESAKVILKAAQKGTFSLINNVKTITKNDEGFSIEKEKQNHISVDRV
                     INATGYQLNTKNWTKANPLLKSLINQEFCQIDDQGGITILTENAQVLSPKYGPMPGLY
                     AHGALVNGVVYQNNSTIKIQQMAERAFSDNK"
     gene            complement(3280..>3872)
                     /locus_tag="VBE34_001866"
     CDS             complement(3280..>3872)
                     /locus_tag="VBE34_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7264546.1"
                     /translation="SGVFYTILGNSGSGKTTLLSLMAGLDSPTEGQVLFNKKDIKEAG
                     YAQHRKKNIALVFQNYNLLDYLTPLENVQLVKPTADKQLLLDLGLKEDMLTRNILRLS
                     GGQQQRVAIARALVVGTPAILLDEPTGNLDFDIARDITMRLKDFAHKEKRCVIMVTHS
                     REIAHMADTALQLIGDNLKELSKESIAQSILQQRNL"
BASE COUNT         1187 a          712 c          619 g         1354 t
ORIGIN      
        1 taaaggagac tcctatgttt ttaatcgatt ttattttaca tattgatact catatctatg
       61 ctatggctaa cactgttggt aattggacct atctattgct cttcctcgtt attttcgttg
      121 aaactggggc tgtcatcttt ccatttttac caggtgatag tttattattt gctgctggag
      181 cccttgctgc aaatccaaaa atgtccttca atattgtaac ttttcttatt attttcttta
      241 ttgcagcatt tattggtgat agttgcaact tcttgattgg tcgtacattt ggttaccgtt
      301 ttatcaaaca tcctttcttt cgtcgtttca ttaaagaaaa aaatatccga gatgctgagc
      361 tttattttga gaaaaaaggt actgctgcca tcattttagg gcgctatatc cctattatca
      421 gaacctttgt tccttttgta gcaagtatta gtcagctccc tccaaaagta tttatcaaac
      481 gcgccttcat tgcagcatta tcttggtcag ttattgcaac aggttctggt tttctctttg
      541 gtaatattcc ttttgtgaag cagcacttct cgcttattat cttgggaatt gtatttgtta
      601 ccttaattcc tgtccttatt tctggtgtca aatcatatcg gaacactaag taatgtatcc
      661 aaaaatttct atttaacact attaatcact aagaatgaca acaaaaaaag agcaattagt
      721 tgctctttta ttaatttagt gttatttctc ctgttgcata atccatagtg tatgatgctt
      781 gtgtgtttgg aatagcaaca ttaaattcta atgtgccatc ttgtgattta gcttctaggt
      841 gcattgcaaa tggaactaaa tcagtatcat tacggtacaa tggagttaca cggtaacgaa
      901 cacgtttgtt ttggtcaacc gccttacgaa ctaagctttc ataataattt tgtccacgat
      961 tgattttttg atttgattgg ttggaatgag ctgtttgtgt gacaacattt tgaggatttg
     1021 acacggaagc atcccaacct ttgaaatttc cagctaaagc ataggcaatt aaatgccccc
     1081 ttgtcgactg catgcccata atggtcatta gtagctactt gatgccaacc taatggtttc
     1141 caatttgcat cgggaagagt ggtatctctg cgattttgat attgacgaat ggttctatca
     1201 agcattgcat tagcagttga tagtctttgt tgattgtcaa ttgtattttg aacatacgct
     1261 ttaacattga catcagcatt taaatttgtc tgattattat ttaattcata aataccatta
     1321 acatattgaa atggtaggtt accaatagca ttttttaaat tattttgaaa ttcctggctc
     1381 gtatcaacag atgaattgac atcagcatgt actatcggcg ttacgttttg cggctgcgtt
     1441 ggaagaccaa aggtcattac ccccaacata cctaatgtag caattacggt tataaacagt
     1501 ttatgtaatc ttttcataag ctttacctct ttctaattac attaaacaag cgtctaccat
     1561 attgatatcc gttttagcta attgctctcc ttttgtcaca accatatcat atgcctatta
     1621 tttaagcatg tcaatcattt atttaatctt atattaattt aatattactt taaatttaat
     1681 ttactcgtta aattagaaaa tctgcaaata atagctttgt tcatccccta acttaaatca
     1741 aaatttatgc tatgatagtg gtaaggaggc ttgttctgtg aaaaaaacaa ttggtattat
     1801 aggtatggga gttagtggtt tagctgttct actagcactt tctcgtaaaa aagagaaaga
     1861 gttaaaacag ttagctattt tttgttttga tgatactgtc cactttggac gaggaattcc
     1921 ttttcaagaa gacgttgact cagctttaat caattcccca atcaacgata tttcatttga
     1981 ttatcgccat atgtctgact tcatggattg gttagaagaa aattactatg acacaaatct
     2041 aacctatgta tcaaggacac tttacggaca atacctttca gaaagaacta agttactttt
     2101 agataaactc cctgtcacgg ttattaaaac aagagtggat aaactaaact atttatcaga
     2161 tgagcaacaa tggcaagtct atattcaaga taagcttttt cctgtcacat ttgatgaatt
     2221 acacttagct tgtggtgctc tgcctgttat agaaccttat catttggaag ggaagaccga
     2281 ttatatcaaa gacccttatc ctatcaaata cttggctcag gaaaattgga agggaaaaac
     2341 agttgctatc atcgggactg gtctagctgc aattgatgtt ataaaatggt tgctactcaa
     2401 tacacaatct tctgttaaag cattttcaag aaccaactac ttccctagcg tccgtattgt
     2461 taatgattta ccacttactt ggcacttttt taccaatgat aaccttaaag catttctatc
     2521 acatccttca aaaaccttca ctattgccga ttttaatgat ttgatgtctg gcgaattgga
     2581 gcatcttggc tttaaaaact ggaaaaatac aacagaaact tttttatccc caggaattga
     2641 tggtctcaag ttagccttta aataccctaa gcaactctat ctattacaac aattagcttc
     2701 acgaatcact gtctggttaa ctgacctttg gccactcatg tcattatcag ataagaaaga
     2761 gtataaagca ctctatggaa aagcgattat taacttgcga aatcctatgc ctgatgaatc
     2821 tgctaaagta attttgaaag ctgcacaaaa agggactttt tctctgatca ataacgtcaa
     2881 gacaattaca aaaaatgacg aaggattttc aatagaaaaa gaaaaacaaa atcatatatc
     2941 tgtcgatcgc gtcattaacg ctactggtta ccagttaaac actaaaaact ggacaaaagc
     3001 caatccactt ctaaaatcgc tcatcaatca agaattttgt caaattgacg atcaaggagg
     3061 gattactatt ttaactgaaa atgcacaagt tttgtctcca aaatatggtc caatgccagg
     3121 tttatatgcg catggtgcac tggttaatgg tgtcgtttat cagaataact ctacaattaa
     3181 aattcaacaa atggcagagc gagccttttc tgataataaa taaccatttc taaaaaatac
     3241 ctcctctttt attatttaag aaagggaggt atttttttat tataaattcc tttgttgtaa
     3301 tatagactgt gctatacttt cttttgataa ttcttttaag ttatctccta tcaattgtaa
     3361 agcagtatca gccatatgag cgatctctct actatgtgtc accataataa cacatcgttt
     3421 ttccttatgc gcaaaatctt taagacgcat cgtaatatca cgcgcaatgt caaaatctaa
     3481 atttcccgta ggttcatcaa gtaaaatagc tggtgttcct acaaccaaag ctctcgcaat
     3541 agctacacgt tgttgttgcc ctcctgataa tctcaagata tttcgcgtta acatatcttc
     3601 ttttaaacct aaatccaaca ataattgttt atctgcagta ggttttacca actgaacatt
     3661 ttccaatggg gttagatagt ctaaaaggtt ataattttga aagaccaaag ctatattctt
     3721 tttgcgatgc tgagcataac ctgcttcttt aatatctttt ttgttaaata atacttgtcc
     3781 ctcagtaggc gaatcaagac ctgccatcaa ggatagaagg gttgttttcc ctgaaccaga
     3841 attccctaat atagtataaa atacccctga ct
//
LOCUS       DATRUK010000101         3754 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.101, whole genome shotgun sequence.
ACCESSION   DATRUK010000101 DATRUK010000000
VERSION     DATRUK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.101"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            54..869
                     /locus_tag="VBE34_001867"
     CDS             54..869
                     /locus_tag="VBE34_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7264547.1"
                     /translation="MIYGFVGPNGAGKTTTIKMILGLLKFDTGFITIFGKKVNFGRTD
                     TNQLIGYLPDVPEYYDYMTALEYLDLCSGLARSKHKLSNKELLRSVGLDDNHQKIATY
                     SRGMKQRLGLAQALVHDPKIIICDEPTSALDPKGRQDILDIISNLRGEKTVIFSTHIL
                     SDVEKICDHVLVLTKCGIYSLEELKGKKSEENYSVRILIKVTKSEAKVLSHNYQIEKK
                     DNEYALTLKGSKMDNKADLLAGFYQDLVSLKISPSAIEVIDNSLEELYLEVTK"
     gene            866..1627
                     /locus_tag="VBE34_001868"
     CDS             866..1627
                     /locus_tag="VBE34_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7264548.1"
                     /translation="MKSLAVMLKKEWMENVRTYKVISILITCSIFGILGPLTALMMPD
                     IMAGILPKKLQGAIPETTYIDSYIQYFKNMNQLGLVILVFLFSSTLTQEFSKGTLINL
                     VTKGLAKKVIILAKFIVITLLWTVSYLLSVVIHFSYTLYYFSNEGSHKLMVYGATWFI
                     GILFISLILFFSVLFRKTLGILLGCLFMVVLCVVSGFFKISKSYNPMLLIQKQGEMLS
                     GKFKVQDLMEPSILVFLLIIMTLLLSMYIFEKSEV"
     gene            1796..2284
                     /locus_tag="VBE34_001869"
     CDS             1796..2284
                     /locus_tag="VBE34_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7264549.1"
                     /translation="MEIKEYENNPYHLAQLVDLINYCQNIEAKLDIKMAEQDDIFQIE
                     NYYQNRKGQFWIALENEKVVGSIALLRIDDKTAVLKKFFTYPKYRGNPVRLGRKLFER
                     FMLFARASKFTRIVLDTPEKEKRSHFFYENQGFKQITRDKLDVDYIFPDRDSRIYVKL
                     LD"
     gene            2806..3141
                     /locus_tag="VBE34_001870"
     CDS             2806..3141
                     /locus_tag="VBE34_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000827864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7264550.1"
                     /translation="MKRNKHLPLTETTYYILLALFEEAHGYAIMKKVEEMSGGDVRIA
                     AGTMYGAIENLLKQKWIKSISSDDRRRKVYIITETGKEIVELETNRLRKLLNTANQLG
                     FGGDGYDKV"
     gene            3128..3736
                     /locus_tag="VBE34_001871"
     CDS             3128..3736
                     /locus_tag="VBE34_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEO7264551.1"
                     /translation="MIKFKLFFKSTEKEEEWIDKVQKSGYTFTGINSFLPAFYYFKRD
                     KEKDKMSIKMDFKKYMSKDDYTNYITLFEDSGWKHISGSRWGGLHYFQKIKPETSSEI
                     FSDQESKSAMAMSYYKYTCEHAFFSLFYFFLLIRTTSITIFDPKSWYLTQGLWEMQGK
                     IFWLGFLFETPFALLRILPMFYFLISGIYYITRAVKPKEIIN"
BASE COUNT         1382 a          468 c          657 g         1247 t
ORIGIN      
        1 cttttggtcc taaaaaaata ttaaataatg tttcttttga tttagaagaa aatatgattt
       61 atggattcgt aggaccaaat ggagcaggta aaacaacgac aataaaaatg attttaggat
      121 tgttaaaatt tgatacgggt ttcataacaa tttttggtaa aaaagttaat tttggaagaa
      181 cagataccaa tcaactgatt ggctatctcc cagatgttcc cgagtactac gattatatga
      241 cagctctaga atatcttgat ttatgttctg gtttagcgag atcaaagcat aagctttcta
      301 acaaagaact acttaggtca gttggacttg acgataatca ccaaaagatt gctacttatt
      361 ctcgaggaat gaaacaacgt cttggattag cccaagcact ggtccatgat cctaaaatta
      421 ttatttgcga tgaaccaacc tctgcattag acccgaaagg tcgccaagat attttagata
      481 ttatttcaaa tcttagagga gaaaaaactg tgattttttc tacccatatt ctctctgatg
      541 tcgaaaaaat atgtgatcac gttctggtgt taactaagtg tgggatttat tctcttgaag
      601 agttaaaagg aaaaaaatca gaggaaaatt attcggttcg tattttaata aaagtgacaa
      661 agtctgaagc taaggtacta tctcataact atcagataga aaagaaagat aatgagtatg
      721 cactgactct caaaggctct aaaatggata acaaagcaga tttactagct ggtttttacc
      781 aagatttagt ttctttaaag attagcccat cagctattga agtcattgac aatagtttag
      841 aggagcttta tttggaggtt accaaatgaa atctttagca gtaatgttaa aaaaagaatg
      901 gatggagaat gttaggacat acaaagttat atctattctg attacttgtt caatatttgg
      961 tatattaggt cccttaactg cacttatgat gcctgatatt atggcaggaa ttttacctaa
     1021 aaaactacag ggagcgatac cagagactac ctatattgat tcttatattc agtattttaa
     1081 aaatatgaat cagcttggtt tagttatttt agtattttta tttagtagta cactgacgca
     1141 agaattttca aaaggaacct taattaatct agttacgaag ggattagcca aaaaagttat
     1201 aatccttgct aaatttatag taattaccct tctatggaca gtgtcatact tattaagtgt
     1261 tgtcatccat tttagttata cgttatatta ctttagtaac gaaggaagtc ataaattaat
     1321 ggtttacggt gcaacatggt ttatcggtat tcttttcata agcttaatac tctttttttc
     1381 agttcttttt cgtaaaactt tagggatttt gttaggttgt ctttttatgg tggtgttatg
     1441 tgtagtatct ggatttttta aaatcagtaa aagctataat cctatgcttc ttatccaaaa
     1501 acaaggagaa atgttaagtg gaaagtttaa agttcaagac ttaatggaac cgagcatatt
     1561 agtgtttttg cttattataa tgacgttatt acttagcatg tacatttttg aaaaaagtga
     1621 agtttaattt tgataagata tcaataaggc gatagctact ttttgaaaaa ataaaaccga
     1681 attaagagct ttgctgctat ttgacttatc tagatcaata tttccaacta atatgatatc
     1741 ctatgcatat ctcattgcag aaatatactt taaagtcaaa ataaggaaat atattatgga
     1801 aattaaagaa tacgagaata atccgtacca tttagcacag ctagtagatt taattaatta
     1861 ttgtcagaat atagaagcta aacttgatat aaagatggct gaacaagacg atatttttca
     1921 aatagaaaat tattatcaaa acagaaaagg tcaattttgg attgcattag aaaatgaaaa
     1981 agtagttggt tcgattgcgt tactgcgtat tgatgataaa acagctgttc taaaaaaatt
     2041 ttttacatat ccaaaatata gaggaaatcc tgttcgactc ggtcgaaaat tatttgaaag
     2101 atttatgctt tttgctcgag ctagtaaatt cacaagaatt gtcttggata ctccagaaaa
     2161 agagaaaaga tctcacttct tttatgaaaa tcaaggattt aagcaaataa caagagataa
     2221 actagatgta gactacattt ttccagatag agattcaagg atatatgtaa aacttcttga
     2281 ttaattgtga agaaaactag ccaaacagat gaataaaccc aaataacgat tattttagat
     2341 aatacattgg aaaagtggtt gtagaatata gaaaaagaaa tttttaggat tacggttatc
     2401 attggttatg gtagcttatt caagtaatat cataagagat gaatcaatgg atataaattg
     2461 aataaaaaat aatagaggcc agagtgatga actctagtct ctattattaa ttttaaaaac
     2521 tattgtgttt atgaggaggt attttaactg tatagattgg ataagaagtt ataattaaag
     2581 gtattaagat caggatgaga tgaataaaca acagtactat agtcaataga ttataaaaaa
     2641 ctatagtact tttgagaagt tacgactaaa ataaaaaact ccaaacgaat aattggatag
     2701 gctatctgtt cttttctttt taataagttt aaatatacag ttgacctata tagttatatt
     2761 atatagaata actatataaa gtttaactgt atagaaagaa aattgatgaa aaggaataaa
     2821 catttaccgt taacagaaac tacctattat attttattag ctttgtttga ggaagcgcat
     2881 ggctatgcta ttatgaaaaa agttgaagaa atgagtggcg gtgatgttag aatagccgca
     2941 gggacaatgt acggtgccat tgaaaattta cttaaacaaa aatggataaa gtctatctca
     3001 agtgacgata gaagaagaaa agtttatatt attactgaga caggaaaaga aatagtagaa
     3061 cttgaaacga atcgattaag aaagttactt aatactgcta atcagttggg ttttggagga
     3121 gatggttatg ataaagttta aattattttt taaatccaca gaaaaagagg aagaatggat
     3181 cgataaggtt caaaaaagtg gctatacttt tacaggcatt aactcatttc ttccagcctt
     3241 ctattatttt aagagagata aagaaaaaga caagatgtca ataaaaatgg attttaaaaa
     3301 atacatgtcg aaagatgact atactaacta tatcacttta tttgaggaca gtggatggaa
     3361 gcatattagc ggtagtcgtt ggggagggct gcattatttt caaaaaataa agcctgaaac
     3421 cagttcagag attttttctg atcaagaatc aaaatcagcg atggctatga gttattataa
     3481 atatacttgt gagcatgcgt ttttctcttt attttatttc tttctcttga taagaacaac
     3541 ttctatcacc atatttgatc caaaaagttg gtatctgacg caaggtctat gggaaatgca
     3601 agggaaaata ttttggttag ggttcttatt tgaaacacca tttgccttat taagaatatt
     3661 acctatgttt tactttttaa tttctggaat atactatatt acacgagctg taaaacccaa
     3721 ggaaattata aattaagcag aagtatttta gatt
//
LOCUS       DATRUK010000102         3595 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.102, whole genome shotgun sequence.
ACCESSION   DATRUK010000102 DATRUK010000000
VERSION     DATRUK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.102"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            18..1688
                     /locus_tag="VBE34_001872"
     CDS             18..1688
                     /locus_tag="VBE34_001872"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEO7264552.1"
                     /translation="MKTDIEIAQSVALKPIAEIVEQVGIGFDDIELYGKYKAKLSFDK
                     IEAVRSQKVGKLILVTAINPTPAGEGKSTMSIGLADALNKIGKKTMIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELDIDQRR
                     VIWKRVVDLNDRALRQVIVGLGSPVNGIPREDGFDITVASEIMAILCLATDLSDLKKR
                     LSNIVVAYSRDRKPIYVKDLKIEGALTLILKDAIKPNLVQTIYGTPALVHGGPFANIA
                     HGCNSVLATSTALRLADYVVTEAGFGADLGAEKFLDIKTPNLPTSPDAIVIVATLRAL
                     KMHGGVSKEDLSQENVEAVKRGFTNLERHVNNMRQYGVPVVVAINQFTADTESEIATL
                     KTLCSNIDVAVELASVWEDGADGGLELAQTVANVIETQSSNYKRLYNDEDTIEEKIKK
                     IVTKIYGGNKVHFGPKAQIQLKEFSDNGWDKMPICMAKTQYSFSDNPNLFGAPTDFDI
                     TVREFVPKTGAGFIVALTGDVLTMPGLPKKPAALNMDVLEDGTAVGLF"
     gene            1807..3402
                     /gene="cls"
                     /locus_tag="VBE34_001873"
     CDS             1807..3402
                     /gene="cls"
                     /locus_tag="VBE34_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEO7264553.1"
                     /translation="MIWRGEKLISNKVKIVRLLNKSKKSLLRGIFSRTTVIAILLILQ
                     LLFLLASYSWLEQYRVWLATVEHILTIGAVLYLVNSEMDALSRVTWLILVMIAPLLGA
                     MFLMYTKFDWGYRGLKQRLETLIDESQIYLEDDPETLNQLKSSTSTTYHLVQYFEKAH
                     GNFPVYRNTDVTFLPTGEAFFEKMKEELLKAKKYIFLEFFIIDEGIMWGEILSILEQK
                     VEEGVEVRILYDGMIEITKLSFDYTKRLEKIGIKAKAFSPISPFISTYYNYRDHRKIV
                     VIDGVVGMTGGVNLADEYINHIELFGHWKDSGIMLKGKAVDSFLLLFLQMWSITEEKM
                     LVAPYLGVHDDLVENEGYVIPYGDSPLDTDKVGENVYIDILNHAREYVYIMTPYLILD
                     SELEHAIQFAAERGVDVRIIMPGIPDKPIPYALAKTYYQALIKSGVKIYEYTPGFVHS
                     KIFLSDNTKAVVGTINLDYRSLYHHFECAVYLYKVDAIQDIYRDYMDTLNKSRLVSLK
                     DINNIPKFQKVIGIVTKTIAPLL"
BASE COUNT         1174 a          532 c          737 g         1152 t
ORIGIN      
        1 tttagaagga gaatatcatg aaaacagata ttgaaatagc gcaaagcgtc gccctaaagc
       61 ctattgctga gattgttgag caagtaggaa ttggttttga tgatatagaa ttgtatggta
      121 aatacaaagc aaaattatct tttgataaga ttgaagctgt aagatctcaa aaagtaggaa
      181 agttaatttt agtaactgct attaatccga caccagcagg tgagggaaaa tccactatga
      241 gcattggatt agctgatgct ttaaataaaa ttggtaaaaa aacgatgatt gcgcttcgcg
      301 aaccttcttt aggtcctgta atgggtatta aaggaggagc tgctggaggt ggatacgcac
      361 aagtactacc aatggaagat attaatttgc attttactgg tgatatgcac gcaatcacaa
      421 ctgctaataa tgctttatca gctctacttg ataatcatat tcatcaaggc aatgaattag
      481 atattgacca acgtcgagtg atttggaagc gtgtagttga tcttaatgat cgtgctcttc
      541 gtcaggtgat cgttggctta ggaagccctg tcaatgggat accacgtgaa gatggctttg
      601 atattactgt tgcttctgaa ataatggcta ttctatgttt agctactgac ctatcagatt
      661 taaagaaacg cctgtcaaat attgttgtgg cttactcaag ggaccgtaaa ccaatctatg
      721 ttaaagattt gaagattgaa ggagctctca cacttatcct aaaagatgct atcaaaccta
      781 atttggttca aaccatttat gggacaccgg ctcttgtcca tgggggacca tttgctaata
      841 ttgcacatgg atgtaattct gtcttagcga cttcaacagc tctacgctta gcagattacg
      901 ttgttactga agctggtttt ggagcagact tgggcgctga aaagtttctt gatataaaaa
      961 cgccaaacct tccgacttct cccgatgcga ttgttattgt tgctacattg cgtgcattga
     1021 aaatgcatgg aggtgtttca aaagaagatc tctcacaaga gaacgtcgaa gcagttaaaa
     1081 gaggttttac aaatctcgag cgtcacgtta acaatatgcg tcaatatggt gttccggtag
     1141 ttgtagctat caatcagttt actgcagata cagaaagtga aattgcaact cttaaaacct
     1201 tatgtagtaa tattgatgtg gcagttgaat tagcaagtgt gtgggaagat ggagcagacg
     1261 gtggccttga acttgcacag actgttgcta atgtgattga aacacaatca tccaattaca
     1321 aacgcttata taatgatgaa gacactattg aagaaaaaat taaaaaaatt gttactaaaa
     1381 tatatggtgg taataaagtt cattttggac ctaaggcaca aatacaatta aaagagttta
     1441 gtgacaatgg ctgggacaag atgcctattt gtatggcaaa gacacaatat agcttttctg
     1501 ataatccaaa tttatttggt gctccaactg actttgatat aactgttcgt gaatttgttc
     1561 caaaaacagg agctggtttt atagttgctc ttactggaga cgtattgaca atgcctggtt
     1621 tacctaaaaa acctgcagct ctcaatatgg atgttttaga agacggtaca gccgttggat
     1681 tattttaaat aataaagagg tagggcttgt gatgctagcc tctttttatt ggggattaga
     1741 ttttaactag agataattta gaataataga atgctattca agtgacagta tgctataata
     1801 aaatgattga tttggagggg ggaaaaactt atcagtaata aagtcaaaat tgttcgttta
     1861 cttaataaga gtaagaaaag tttactaagg ggtatattta gtcgaacaac cgttatcgct
     1921 attttattga ttttacaatt attgttttta ctagcctctt actcatggct agagcagtat
     1981 cgtgtttggt tagcaaccgt tgagcatatc ttaacaattg gagcggtctt atatttagtt
     2041 aatagtgaaa tggatgcttt gtcccgagta acttggttaa tattggtaat gattgcccct
     2101 ttactagggg cgatgttcct aatgtacacc aaattcgact ggggttatcg tggtttgaaa
     2161 caaagattgg agacattgat tgatgagagt cagatttatc tagaggatga tcctgaaact
     2221 ttgaatcagc ttaagtcgag tacatccaca acatatcatt tggttcaata ttttgaaaaa
     2281 gcacatggaa atttcccagt ttatcgaaat actgatgtga catttctacc aacgggagaa
     2341 gcattctttg aaaaaatgaa agaagaacta ctaaaagcta agaagtatat ctttttagaa
     2401 ttctttatca ttgacgaagg aataatgtgg ggagaaatac ttagtatttt agaacaaaag
     2461 gtggaagaag gtgttgaagt ccgtatctta tatgatggta tgatagaaat tacaaaatta
     2521 tcctttgatt atacgaaacg acttgagaaa ataggaataa aagcaaaagc attttctccg
     2581 atttctcctt ttatttcaac ttattataac tatcgtgacc atcgaaaaat tgttgttatt
     2641 gatggagtag taggcatgac tggtggtgta aacttagccg atgagtatat taatcacatt
     2701 gagttatttg ggcattggaa agattcaggt attatgttaa aggggaaagc agtggatagt
     2761 ttcctcctac ttttcttaca gatgtggtcc attactgagg aaaaaatgtt agtagcgcct
     2821 tacctcggtg tacacgatga tttagttgaa aatgaaggct atgttattcc ttatggagat
     2881 tctcctttag acactgataa agtaggggaa aatgtttata tagatattct taatcatgct
     2941 agagaatatg tttacatcat gacaccttat ttaatattag acagtgaatt ggaacatgcc
     3001 attcagtttg ctgcggaacg tggtgtagat gtacgtatta ttatgccggg tatcccagat
     3061 aagccaatcc cttatgctct tgctaaaact tactatcaag cattaataaa atcaggtgtt
     3121 aaaatatatg aatacacccc tggatttgtt cattcaaaaa tattccttag tgataataca
     3181 aaggctgttg taggtacgat aaatcttgat taccgtagcc tgtatcacca ttttgaatgt
     3241 gctgtttatc tttataaggt agatgcaatc caagacattt atcgagatta tatggatacg
     3301 ttaaataaat caaggttagt tagcttgaaa gatataaata atattccaaa attccaaaaa
     3361 gtaattggca tagtgacaaa aactattgct cccctgcttt agaaataatt tatttttaat
     3421 ttaatattaa aagtaaactg aagaatctag ttatatttaa aaagtaaagg ttgcatttta
     3481 actaaattat gttaaactac tgttatgcga tgagtcgata tgtggtttta ccactattgc
     3541 gcagggagat tataaacgca ggagcggatc ttgataagtt gtgtgaacct tctta
//
LOCUS       DATRUK010000103         3477 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.103, whole genome shotgun sequence.
ACCESSION   DATRUK010000103 DATRUK010000000
VERSION     DATRUK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.103"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            2..72
                     /locus_tag="VBE34_001874"
     tRNA            2..72
                     /locus_tag="VBE34_001874"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34..36,aa:Gly,seq:tcc)
     gene            109..182
                     /locus_tag="VBE34_001875"
     tRNA            109..182
                     /locus_tag="VBE34_001875"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:143..145,aa:Ile,seq:gat)
     gene            193..264
                     /locus_tag="VBE34_001876"
     tRNA            193..264
                     /locus_tag="VBE34_001876"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:226..228,aa:Glu,seq:ttc)
     gene            278..367
                     /locus_tag="VBE34_001877"
     tRNA            278..367
                     /locus_tag="VBE34_001877"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:314..316,aa:Ser,seq:tga)
     gene            378..451
                     /locus_tag="VBE34_001878"
     tRNA            378..451
                     /locus_tag="VBE34_001878"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:412..414,aa:Met,seq:cat)
     gene            454..526
                     /locus_tag="VBE34_001879"
     tRNA            454..526
                     /locus_tag="VBE34_001879"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:487..489,aa:Phe,seq:gaa)
     gene            538..618
                     /locus_tag="VBE34_001880"
     tRNA            538..618
                     /locus_tag="VBE34_001880"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:572..574,aa:Tyr,seq:gta)
     gene            625..695
                     /locus_tag="VBE34_001881"
     tRNA            625..695
                     /locus_tag="VBE34_001881"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:657..659,aa:Trp,seq:cca)
     gene            710..782
                     /locus_tag="VBE34_001882"
     tRNA            710..782
                     /locus_tag="VBE34_001882"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:743..745,aa:His,seq:gtg)
     gene            789..860
                     /locus_tag="VBE34_001883"
     tRNA            789..860
                     /locus_tag="VBE34_001883"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:821..823,aa:Gln,seq:ttg)
     gene            871..954
                     /locus_tag="VBE34_001884"
     tRNA            871..954
                     /locus_tag="VBE34_001884"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:905..907,aa:Leu,seq:caa)
     gene            1330..1630
                     /locus_tag="VBE34_001885"
                     /pseudo
     CDS             1330..1630
                     /locus_tag="VBE34_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865753.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1870..2073
                     /locus_tag="VBE34_001886"
     CDS             1870..2073
                     /locus_tag="VBE34_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264554.1"
                     /translation="MAESANTVRSNSFVLEELTEFQREKLKNGESVKFNFSKDVKKGN
                     QITQGGSNLPNDGSVYLQPNKSL"
     gene            2224..2664
                     /locus_tag="VBE34_001887"
     CDS             2224..2664
                     /locus_tag="VBE34_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein BUG3"
                     /protein_id="HEO7264555.1"
                     /translation="MKKHIKSIIPIVLIGMILGGCQMNSEHKSQYNETKSSKQSEVKK
                     DKKMTKKEQLAYLKEHEQEIIDFVKSQNKKIESVQIDWNDVRWSKGGNGTPQGGGEGI
                     LLFGEINNDSESSWRVDIDIEKGRLDLKNMYLGQPIRIGGKLFE"
     gene            2657..3001
                     /locus_tag="VBE34_001888"
     CDS             2657..3001
                     /locus_tag="VBE34_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264556.1"
                     /translation="MSNWNLESFDNLFATLAESTYTKRPSNFTYNELNPKLLRQLNSG
                     SSVELDFSKSVKKGKKVIEGGQNLPNDGIVYLQPDKSLKSIDEKVEVRVPDVNGGYHS
                     EHCVTNSYQKGY"
BASE COUNT         1167 a          515 c          717 g         1078 t
ORIGIN      
        1 agcgggtgta gtttagtggt aaaactacag ccttccaagc tgttgtcgcg agttcgattc
       61 tcgtcacccg ctttacttta taagaaagta aaaaccaagc atttgcttgg gcgcgtagct
      121 caggtggtta gagcgcacgc ctgataagcg tgaggtcggt ggttcgagtc cactcgtgcc
      181 catcaagaat atggtccgtt ggtcaagggg ttaagacacc gccttttcac ggcggtaaca
      241 cgggttcgaa tcccgtacgg actatttttt gttatatgga ggattaccca agtccggctg
      301 aagggaacgg tcttgaaaac cgtcaggcgt gtaaaagcgt gcgtgggttc gaatcccaca
      361 tcctccttta aagatatcgc gggatggagc agctaggtag ctcgtcgggc tcataacccg
      421 aaggtcgtag gttcaaatcc tgctcccgca ataggctcgg tagctcagtt ggtagagcaa
      481 tggattgaag ctccatgtgt cggcggttcg attccgtctc gcgccattta taccttagga
      541 gagatagcga agaggctaaa cgcggcggac tgtaaatccg ctccttcggg ttcgggggtt
      601 cgaatccctc tctctccata gtaaacgggc atagtttaaa ggtagaacta aggtctccaa
      661 aaccttcagt gtgggttcga ttcctactgc ccgtgttttt tctaatatgg cgggtgtggt
      721 gaagtggtta acacatcaga ttgtggctct gacattcgtg ggttcgattc ccatcactcg
      781 ccttttcatg gggtatagcc aagcggtaag gcaagggact ttgactccct catgcgttgg
      841 ttcgaatcca gctaccccag ttttaagata gccggcgtgg cggaattggc agacgcgctg
      901 gactcaaaat ccagtgtcct cacggacgtg ccggttcgac cccggccgcc ggtatacttt
      961 aaaagacaag gtttctaaac cttgtctttt tttgttttgt actggtaatt ttaagaaaaa
     1021 gttactgaaa attacttata tttgtttttc attattgcaa ttgttattga agttttaaaa
     1081 caagcagaaa taataaggaa atttttcagg agttagtgga gactcaacat ttaaatgaaa
     1141 ttcaaacagg actcctaaat gtcataggtt ctgaagtaat aaaagtcttt gtgaatgata
     1201 gataggtagt ataaaataat aagatttttc agtgtatatg ttacgactaa aaaattaaac
     1261 tcgtgaaaat ttgttatact aatattagtt ttaaaatact ggtaaataat aattattttt
     1321 attagatact tgacatggga agtttatttt ctaagaaaag agagcatgat gaaaaaatat
     1381 gtaaaatggt taattcccat atctattatt gggatgttac tagcaggttg tcaaatgaat
     1441 agtgaatcta aaagtcagtc aaacgaagta aaaaatagca aacagtcagc agtaaagaaa
     1501 ggtaaaaaag taataaaaaa agaacaagta gcttatctca aaaagtatga aaaagaatta
     1561 actgaatatg tcaaaaccca aaatccagat attagcgaaa ttaagtatga ttggaatagt
     1621 gtgcgtatag tggaagctag taatggaacc cctcaaggcg gagagcagct attattaatt
     1681 tggggttatg caaatggcag tgaattaacc aattttactc taaatttcac attgggtgat
     1741 aatcaaatgc ctaatattga ctcagttggc tcagataatt tatcaactat tgaagattaa
     1801 taataacagt aagaaaggta tataatggga tgttggaatc ttaaggaatt tgaagatata
     1861 ttttgaagta tggcagaaag tgctaataca gttcgatcga attcttttgt tttagaagaa
     1921 ttaactgaat ttcaaagaga aaagttaaaa aatggggagt cagttaagtt taatttttct
     1981 aaagatgtta agaaaggaaa tcaaatcacc caaggtggct caaatctccc aaatgatggg
     2041 agtgtctatc ttcagcctaa taagtcctta taaactgttt attaaactga aaaaattgaa
     2101 gttttcatag ataaaattct tcgagtaaaa tattagagat gctatttata tttcttaagc
     2161 tagtaatttt ttattgttta ttggtttaat gataatacaa atataaaata ggtggtgcaa
     2221 ataatgaaaa aacatataaa aagtatcata ccaatagttc ttattggtat gatactagga
     2281 ggctgtcaaa tgaatagtga acataaaagt cagtataatg aaacaaaaag tagcaagcaa
     2341 tcagaagtga agaaagataa aaaaatgaca aaaaaagaac aattagctta tctcaaagag
     2401 catgaacaag aaataattga ttttgtaaaa tctcagaata aaaagataga atctgtacaa
     2461 attgattgga atgatgttcg atggagtaaa gggggaaatg gtacacctca aggaggagga
     2521 gaggggattt tactttttgg ggagattaat aatgattctg aatcaagttg gagagttgat
     2581 attgatatag aaaaaggacg gctagaccta aaaaatatgt atttaggaca acctatacga
     2641 attggaggta aattatttga gtaattggaa cttagaaagt tttgataacc tatttgctac
     2701 tttagcagaa tcaacataca caaaaagacc aagtaacttt acatataatg aattgaatcc
     2761 taagttgcta cgacaactga actcaggtag ttctgttgaa ttggatttct caaaatctgt
     2821 taagaaagga aaaaaagtta tcgaaggcgg tcagaacctc cccaatgatg gtattgtcta
     2881 cctgcaacca gataagtcct taaaatctat tgatgaaaaa gtagaggttc gcgttcctga
     2941 tgtcaatggc ggttatcact ctgagcattg tgttactaat tcctatcaga aaggttactg
     3001 aaagatgatg aagcagtggc tctataatca ctaaaagctc atcattgctc agtgtttaag
     3061 ttaacttcta aagctcgaac cttgttaaat caaggtttgt ttacttttaa aagtgacatt
     3121 ttatttttct aaaaataagg ttatcgggat tcctcattct aagttacttt gtttatatgt
     3181 acctttactt aaggtttatt ttcattattt ttaatttaat attttggagt atgtacgctc
     3241 cattatagta ttttcgaaga catctttaaa agaactgtag aagttaaaat attttaacct
     3301 agtatctaac gtcatatatt ggtacatagt tttaaatatc cttttgataa ccttgtacat
     3361 atgaaatagg ctaaatattt ttagtgaact aaatgtaagt ttattgtttg tattaggcga
     3421 gataacaata ttgttgtttt tattctttct gagtaatggt ttattaatta tgtaatt
//
LOCUS       DATRUK010000104         3185 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.104, whole genome shotgun sequence.
ACCESSION   DATRUK010000104 DATRUK010000000
VERSION     DATRUK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.104"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            188..261
                     /locus_tag="VBE34_001889"
     tRNA            188..261
                     /locus_tag="VBE34_001889"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:222..224,aa:Arg,seq:tct)
     gene            complement(334..813)
                     /gene="rlmH"
                     /locus_tag="VBE34_001890"
     CDS             complement(334..813)
                     /gene="rlmH"
                     /locus_tag="VBE34_001890"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEO7264557.1"
                     /translation="MKLKIITVGKLKEKYLKEGVAEYQKRLNRFSKIETIELADEKTP
                     DKASISENQRILDIEGERILSKIGERDYVIGLAIEGKQLPSESFSHLIDQKMISGYST
                     ITFVIGGSLGLSQKVKKRADYLMSFGLLTLPHQLMKLVLMEQIYRAFMIRQGTPYHK"
     gene            1014..2243
                     /locus_tag="VBE34_001891"
     CDS             1014..2243
                     /locus_tag="VBE34_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEO7264558.1"
                     /translation="MKKKLVSSLLKCSLIIIVSFAGGAFASFVMNHNDNIPNGGVTKT
                     SKVNYNNITPTTKAVKKVQNSVVSVINYKQQESRSDLSDFYSHFFGNQGGNTDKGLQV
                     YGEGSGVIYKKDGKNAYVVTNNHVIDGAKQIEIQLADGSKAVGKLVGSDTYSDLAVVK
                     IPSDKVSNIAEFADSSKLNIGETAIAIGSPLGTEYANSVTQGIVSSLKRTVTMTNEEG
                     QTVSTNAIQTDAAINPGNSGGALINIEGQVIGINSSKISSTSNQTSGQSSGNSVEGMG
                     FAIPSNDVVKIINQLESNGQVERPALGISMAGLSNLPSDVISKLKIPSNVTNGIVVAS
                     IQSGMPAQGKLKKYDVITKVDDKEVASPSDLQSLLYGHQVGDSITVTFYRGENKQTVT
                     IKLTKTSKDLAKQRANN"
     gene            2341..3114
                     /locus_tag="VBE34_001892"
     CDS             2341..3114
                     /locus_tag="VBE34_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEO7264559.1"
                     /translation="MEYLETININHIAPNPYQPRLEFNTKELEELADSIKINGLIQPI
                     IVRPSAVFGYELVAGERRLRAAKLAKLESIPAIIKSYNNDDSMQLAIVENLQRSNLSP
                     IEEAKAYSQLLQKKSMTHEELAKYMGKSRPYISNTIRLLNLPPLITSAIEEGKLSSGH
                     ARALLSLPDASQQKDWYQRILTEDISVRRLEKLLKQEKKTNHKSLQNKDVFLKHQENE
                     LAQFLGSKVKLTINKDGAGNIKIAFNNQEELNRIINTLK"
BASE COUNT         1120 a          542 c          525 g          998 t
ORIGIN      
        1 gttaggtgtt tccttagtac tcttaatatt atatcagaaa aaaaaggttt gtctatactt
       61 ttgttcaata atttttacaa aaaaattgaa cctacttatg agcaaaaaaa tatatttcca
      121 actcataaat agtacatatg aaaacttgac agcctgccta atattttggt agaataacag
      181 agttgatggt tccatagctc agctggatag agcattcgcc ttctaagcga acggtcgcag
      241 gttcgaatcc tgctggaatc agataatgaa agtctagata gattatctag gcttttttat
      301 ttgtttttag cggttgtttc ataaatggaa cgattatttg tggtaaggag tgccttgacg
      361 aatcataaaa gcacgataaa tttgttccat caagactaat ttcataagtt gatgaggtaa
      421 ggttaacagt ccaaaactca ttaaataatc agcacgtttt ttcacttttt gagataaacc
      481 caaactacca ccaataacaa aagtaatagt agagtagccg gatatcattt tttgatcgat
      541 tagatggcta aatgattcag atggcaattg tttcccttct atagccaaac caatgacata
      601 atccctctca cctattttac tcaaaatacg ttccccttct atatctaaaa ttctttgatt
      661 ttcagatata ctagctttat caggtgtctt ttcatcagct agctcaattg tctcaatttt
      721 agaaaagcga ttcagacgtt tctgatactc tgcaacacct tctttaaggt atttctcttt
      781 caattttcca actgtaatta tttttaattt catagctaca ttataacata atccacaata
      841 ttttcacaat ttatccacat gtttatcctt acatatcccc ttattttata ggcaatctat
      901 aactataatc aaggttatcc acaacttgtg gatatttcct ttttttagct tttaaggtat
      961 aattaagaag cttgtcataa gattagtata aaaatttacg gaggtagcta tttgtgaaaa
     1021 aaaaattagt ctcatcactt ctaaagtgtt ctctaatcat tattgttagc tttgctggtg
     1081 gagcatttgc tagttttgtc atgaatcata atgacaatat tccaaatggt ggtgtcacta
     1141 aaactagtaa agtaaattat aataacataa cgcctacaac aaaagctgtt aaaaaggtac
     1201 aaaatagtgt tgtttctgtt atcaattata aacaacaaga gagtcgttct gacctatcag
     1261 acttctatag tcattttttt ggtaatcagg ggggcaacac tgataagggc ttacaagttt
     1321 acggtgaagg ctctggagtc atctataaaa aagatggtaa aaatgcctat gttgtcacta
     1381 ataaccacgt cattgatggg gctaaacaaa ttgaaattca actagctgat ggctcaaaag
     1441 cagttgggaa acttgttggg tcagatacct actctgattt agccgtcgtc aaaattccat
     1501 cagataaagt ttcaaatatt gcagaatttg ctgattcatc aaaactcaac attggtgaaa
     1561 ctgctatagc gatcggaagc cctcttggaa ctgagtatgc aaattctgta actcaaggta
     1621 ttgtatctag tttaaaaaga actgtaacaa tgactaatga agaaggacaa acagtttcta
     1681 caaatgctat ccagacggat gctgctatca atcctggtaa ttcaggtgga gcacttatca
     1741 atattgaagg acaggttatt ggaattaatt ctagtaaaat ttcttctaca tcaaatcaaa
     1801 cctcaggaca atcgtcagga aatagcgttg aaggtatggg atttgccatt ccttcaaatg
     1861 atgttgttaa gattatcaat caacttgaga gtaacggaca agtagagaga cctgctctag
     1921 gtatttctat ggctggatta agtaatttac catccgatgt tattagtaaa ctgaaaatcc
     1981 caagtaatgt tactaatggt attgtagtag catctatcca atctggcatg ccagctcaag
     2041 gcaaactaaa gaaatacgat gtcattacta aagttgacga taaagaagta gcatctccaa
     2101 gtgatttaca aagtttactc tatggccacc aggtagggga ttccataaca gtaacctttt
     2161 atcgtggtga aaataaacaa acagtcacta taaaacttac taaaactagt aaagatttag
     2221 ctaaacaacg agcaaataac taaatcaatt ctagttattt taactaagat atagtaaaat
     2281 taaaggggtt ggtcagatag accaatccct ttagtaagtc tatagaatct gaggtctaaa
     2341 atggaatatt tagaaacgat caacatcaat catattgctc caaatccgta tcaacctcgc
     2401 ctagaattta atacaaaaga actagaagaa ttagccgact ctattaaaat taatggcctt
     2461 attcaaccca ttattgtaag accgtcagca gtctttggtt acgaactggt agccggagaa
     2521 cgacggttaa gagccgcaaa gttagctaaa ttggaaagta tacctgcaat tataaaatcc
     2581 tataataatg atgatagtat gcaattagct attgtcgaaa atttacaacg atctaatttg
     2641 tcacccattg aagaggctaa agcctactct caacttctac aaaagaaaag tatgacacat
     2701 gaagagttag caaaatatat gggaaaatct cgtccctata tatcaaatac gatacgacta
     2761 ctaaatttac cgccactaat tacttcagct attgaagaag gaaaactttc ctctggtcat
     2821 gcgcgtgcac tactcagtct accagatgct agtcaacaaa aagattggta tcagagaatc
     2881 ctcactgaag atattagtgt aagacgacta gagaagttat taaaacaaga aaagaaaaca
     2941 aatcataagt cacttcaaaa taaagatgtc tttctaaaac atcaagaaaa tgagttagct
     3001 caatttctag gtagcaaagt gaaacttact attaataaag atggtgctgg caatataaag
     3061 attgctttca ataaccaaga agaattaaac agaattatca acactctaaa ataagggtgt
     3121 tgattatttt tttatttaat caaacttatc cacaaggtat tttgctattt ttctgttgat
     3181 tctct
//
LOCUS       DATRUK010000105         3154 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.105, whole genome shotgun sequence.
ACCESSION   DATRUK010000105 DATRUK010000000
VERSION     DATRUK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.105"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            76..648
                     /locus_tag="VBE34_001893"
     CDS             76..648
                     /locus_tag="VBE34_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO7264560.1"
                     /translation="MRKRSILPILAVLFVFMSIFSVYSIFHGDKAKEKASVIKQASQT
                     SQTSKKEVLQKKTYPNLNKYSNLEIHVSSTRQTMTITSSDKVIFKTIVSTGAKESPTP
                     KGTFVIEPERGDFFYNASSKEGAYYWVSFKEHGIYLFHSVPTDQQGNEIPEEAKQLGK
                     AASHGCVRMSRADAKWFYENIPQGTTVTIK"
     gene            924..1775
                     /gene="ylqF"
                     /locus_tag="VBE34_001894"
     CDS             924..1775
                     /gene="ylqF"
                     /locus_tag="VBE34_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEO7264561.1"
                     /translation="MATIQWFPGHMSKARRQVQENIKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADPIRTKEWRDFYESQGLKTLAINSKEQSTVKKVTDIAK
                     ILMSDKIAKLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTYY
                     PDRLKERFKSINLEDESPEIIMTLTQKLGYRDDYDRFYNLFVKEVRDGKLGRYTLDIV
                     GEHDGNN"
     gene            1762..2523
                     /locus_tag="VBE34_001895"
     CDS             1762..2523
                     /locus_tag="VBE34_001895"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEO7264562.1"
                     /translation="MATIKEIKAILETIVDLKDKRWQEYQTDSRAGVQKAILQRKKNI
                     QSHLDEEARLEQMLVYEKKLYIEHINLIAGIDEVGRGPLAGPVVAAAVILPPNCKIKH
                     LNDSKKIPKKKHQEIYQNILDQALAVGIGIQDSQCIDDINIYEATKHAMIDAVSHLSV
                     VPEHLLIDAMVLDLPIPQTKIIKGDANSLSIAAASIVAKVTRDKIMSDYDSKYPGFAF
                     SKNAGYGTKEHLEGLQKYGITPIHRKSFEPIKSML"
     gene            2544..3092
                     /locus_tag="VBE34_001896"
     CDS             2544..3092
                     /locus_tag="VBE34_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEO7264563.1"
                     /translation="MTEKEKMLAGQYYRPSAPELRKDREVALKNMQAFNNEDDSSKRN
                     VILQKWFGATGKSIHMEQRFVCDYGCNIYVGENFYANFNQTFLDVCEIRIGDNCMFGP
                     NCQLLTPLHPLDPIERNSGLEYGAPIQIGNNVWLGGGVTILPGVVLGDNVVVGAGSVV
                     TKSFENNVVIAGNPAKIIKKLA"
BASE COUNT         1160 a          520 c          574 g          900 t
ORIGIN      
        1 aaatatttga caaccttctt tctgaaactt ataatagatt tgtccataac agagcaaaag
       61 agagagggat agtacatgag aaaacgttca attttgccaa tattagctgt cttatttgtt
      121 ttcatgagta tctttagcgt atactctatt tttcatggtg ataaagctaa agaaaaagca
      181 agtgtcataa aacaagcatc tcaaacaagt caaacttcaa aaaaagaggt cttacaaaag
      241 aagacttatc caaatctaaa taaatattca aatctagaaa ttcatgtttc atctacgagg
      301 caaacaatga caataacctc gagtgacaag gtcatcttta aaacgattgt ttcaacagga
      361 gcaaaagaga gccctacccc aaaaggaact tttgtcatag aaccggaacg aggagatttt
      421 ttctataacg cctcatcaaa agaaggagcc tattattggg tctcctttaa agagcatggt
      481 atttatctct ttcatagcgt tccgactgat caacaaggaa atgagatccc agaagaagca
      541 aagcaattag gaaaagccgc ctcacatggc tgtgtgcgta tgtcaagggc ggatgcaaaa
      601 tggttctatg aaaatattcc tcaaggaacg actgttacca tcaaatagtc ttaatgtggt
      661 aaaaatcatg ttattaaaag cttggagtaa acttccagag ctttttcgta tatcccttaa
      721 actcaattag taaacgaaac ctatcagcgg aataaatgta taaagtattt cgatatcatt
      781 aaaaaaatga tagaataata gtgttaatcg aatattttat cgacctttat atgcggttga
      841 agaagaataa aactaaaaga ttagacctct aacaaataga tgtagacttt taaggtagtc
      901 tatacataag gagaatttat ttcatggcta ctattcaatg gttcccagga cacatgtcca
      961 aagctcgccg tcaagttcaa gaaaatatta agcatgttga ttttgttaca attttagtgg
     1021 atgcaaggtt acctctttct agtcaaaatc caatgctaac caaaatcgtc ggagacaaac
     1081 ccaaactaat gatcctcaat aaagctgatc tagcagatcc cattcgaact aaagaatgga
     1141 gagattttta tgaatctcaa ggtttaaaga cattagctat taattctaaa gaacaatcaa
     1201 cagttaaaaa agtaaccgac atcgctaaaa tattaatgtc cgataaaatc gctaaactac
     1261 gggaacgtgg tattcaaaaa gagacacttc gtacgatgat tattggaatt ccgaatgcag
     1321 gcaaatcgac tctaatgaat cgcctagcag gtaaaaaaat tgcagtggtt ggtaataaac
     1381 caggtgtcac aaaaggtcag caatggctca aatctaataa ggaactggaa attttggaca
     1441 ctcctggtat cttgtggccc aaatttgaag atgaattagt tggattaaag ctagcattaa
     1501 caggggcaat caaagatcaa ttattgccta tggatgaggt aacaatattt ggattgaatt
     1561 actttaaaac ctactatcct gatcgtctca aagagcgttt taaaagtatt aatttagagg
     1621 atgaatctcc tgaaatcatc atgaccttaa cacaaaaatt aggttatcgt gacgattacg
     1681 atcgctttta taatttattt gtcaaagaag ttcgcgatgg taaattagga cgctatacgt
     1741 tagatatcgt cggagaacat gatggcaaca attaaagaaa tcaaagcaat tttagagact
     1801 attgttgatt taaaagacaa acgttggcaa gaatatcaaa cagattcacg cgcaggtgtt
     1861 caaaaagcaa tccttcaacg aaaaaaaaat attcagtcac atttagacga agaagcgcgc
     1921 ttagaacaaa tgttggtcta cgaaaaaaaa ctttacattg aacatataaa ccttattgca
     1981 ggaatcgatg aagtaggtag aggtccttta gcaggaccag tagtagctgc tgctgttatt
     2041 cttccaccaa actgtaaaat aaaacatctc aatgactcca aaaaaattcc aaagaaaaag
     2101 catcaagaaa tctatcaaaa tattttagat caagcgttag cagttggtat tggtattcaa
     2161 gacagccaat gtattgatga tatcaacatt tatgaagcta caaaacatgc tatgattgat
     2221 gcagtttctc atttatctgt agttcctgaa catcttttaa ttgatgctat ggttttggat
     2281 ctaccaattc cacaaactaa aattatcaaa ggtgatgcta attcactctc tattgcagca
     2341 gcttcaattg tagctaaagt gaccagagat aaaataatgt ctgattatga tagcaaatat
     2401 cctggatttg cattttctaa aaatgctggc tatggtacta aagagcattt agagggtttg
     2461 caaaaatacg gtattactcc gatacatcgt aaaagttttg aacctataaa gtcaatgttg
     2521 taaataatac ggaagaggaa aagatgaccg aaaaagaaaa aatgttagca ggacaatatt
     2581 atcgtccatc agcacctgaa ctacgcaagg atagagaagt tgcactaaaa aatatgcaag
     2641 cctttaacaa tgaagatgat agtagcaaac gtaacgtcat tttacaaaaa tggtttggtg
     2701 ctactgggaa gagtatccat atggaacaac gttttgtctg cgattatggt tgtaatattt
     2761 atgttggtga aaacttctac gccaatttta accaaacttt cttagatgtt tgtgaaattc
     2821 gtattggcga taactgtatg tttggcccaa actgtcaact cctcacccca ctccatcctc
     2881 tagatccaat agaaagaaat tcaggattag aatacggtgc cccaatccaa atcgggaata
     2941 atgtctggct aggtggagga gtgactattt taccaggtgt tgtattagga gataatgtcg
     3001 tcgttggcgc tggaagtgtc gttacgaaat catttgaaaa taatgttgtt atagcaggaa
     3061 accctgctaa gataatcaaa aaattagctt aatgtaagaa aaaaccttta attataacaa
     3121 aggttttttc taatttaaaa aatagacaaa gaga
//
LOCUS       DATRUK010000106         3116 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.106, whole genome shotgun sequence.
ACCESSION   DATRUK010000106 DATRUK010000000
VERSION     DATRUK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.106"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..750
                     /locus_tag="VBE34_001897"
     CDS             <1..750
                     /locus_tag="VBE34_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti sigma factor C-terminal domain-containing
                     protein"
                     /protein_id="HEO7264564.1"
                     /translation="YPNISYDSLYYQPSGQFTGKVHADRFKDIDGVQIPYSSFEENYS
                     ISGTFGTNADEKSEKNGLYDRGTRQKVPQFFNKNVKYKKGEAKTTPTNDLKYVNKLNN
                     DLIEIAITFDKSYSYKEIKTMIPNNIKQNWLWIGTSTELDTSYWPTKYQFGTDPETLN
                     TPQIFIDKIKENLKGNSKVYFNNINIYSDLEKYAKKYDKVNNSDKLKFSGIILTGKAE
                     NFKQLKEKEWIHASSIGANIEYKPYYKLDKE"
     gene            complement(1010..1690)
                     /locus_tag="VBE34_001898"
     CDS             complement(1010..1690)
                     /locus_tag="VBE34_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7264565.1"
                     /translation="MGDVVENFTEGKNPKIDTLNGKTVRIEKINPDHFEDLFQVYGEL
                     STEDSLTYMSFSKFNSKNEFDVFFQTLLKSEDPYYLAIVDNNTGKALGTFSLMRIDTK
                     NRVVEMGWVVYSSKLKQTRIATEAQYLVMKYVFEELCYRRYEWKCDSLNAPSNNSAKR
                     LGFTFEGTFRQAVVYKGRNRDTNWYSILDKEWPEKKTRFERWLDDSNFDVNEYQIRSL
                     SSIEQKTK"
     gene            complement(1765..2174)
                     /locus_tag="VBE34_001899"
                     /pseudo
     CDS             complement(1765..2174)
                     /locus_tag="VBE34_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(2416..2976)
                     /locus_tag="VBE34_001900"
     CDS             complement(2416..2976)
                     /locus_tag="VBE34_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7264566.1"
                     /translation="MNKFFLESERLIIRPLEESDYDSWYTSFMNRGPSLTPFDDGILD
                     MSMCDINWFKNLVNLQRKEWESDIVYIFGVFLKSGEHVGMLNIVTLARADMQWGELGY
                     VFHNQFWSNGYAFESILALLNSTYEKLGFHHIEAQITPGNERSEKLVRRLGLTYETTR
                     KDFSFENGKWTDKLIYSINLHNNNLE"
BASE COUNT         1134 a          530 c          358 g         1094 t
ORIGIN      
        1 tatccaaata tttcctatga tagtctctat taccagccaa gcggtcagtt tactggtaaa
       61 gtacatgctg atagatttaa agatatcgat ggggttcaaa ttccatattc tagttttgaa
      121 gaaaattata gtatctcagg tacttttggc acaaatgctg atgaaaaatc agaaaagaat
      181 ggcttgtatg atcgaggtac tagacaaaaa gttccacaat ttttcaataa aaatgttaag
      241 tataaaaaag gagaagccaa aacaactcca acaaatgatt taaaatatgt aaataaattg
      301 aataatgatc ttattgaaat tgcaataact tttgataagt cctatagtta caaagaaata
      361 aaaacaatga taccaaataa tataaaacaa aattggttat ggataggtac aagcacagaa
      421 ttagatacat catattggcc aacaaagtat caattcggta cagatcctga aactttgaat
      481 acaccacaaa tattcataga taaaataaaa gaaaacttaa aaggtaactc aaaagtatac
      541 tttaataata taaatatcta ctcagattta gaaaagtatg ccaaaaaata cgacaaggta
      601 aataatagcg acaaattaaa attttcggga ataatattaa caggtaaagc cgaaaacttt
      661 aaacaactta aagaaaaaga atggatacat gcttcaagta taggggcaaa catagaatat
      721 aagccctatt ataaattaga taaagaatga taattaattg ttaaaaaatt tgtttggtaa
      781 ttttacgtaa cttttacagc taataactca tagaaaaaaa gacaagataa taatcttgac
      841 ttttttcttt gatgcccgcg accaaagtcg agctagcacg tccgtgagga cacccgattt
      901 tgagtcctac catgcacctt taaaagttaa taaacattta tttaacaaag tttaagatgt
      961 tagctaaaaa aacatgtaat tttgaaattt tagtgattaa tgaatgactc tattttgtct
     1021 tctgctcaat gcttgatagt gatctgattt gatactcatt tacatcaaag tttgaatcat
     1081 ctagccatct ttcaaaccgt gtttttttct ctggccattc tttatctaaa atagaatacc
     1141 aatttgtatc tctgttacga cccttatata caacagcttg tctaaaagtt ccttcaaaag
     1201 taaaaccaag tcgttttgca gaattattag atggtgcatt caaactatca catttccact
     1261 cataacggcg gtaacagagt tcttcaaaaa catatttcat aaccaaatat tgagcttctg
     1321 ttgctatcct tgtttgtttt agttttgatg aataaaccac ccatcccatt tcaacaactc
     1381 tattttttgt atcaatacgc attaatgaaa aagtccctaa tgctttacca gtattattat
     1441 caacaatagc taaataataa ggatcttctg attttaatag agtttggaaa aagacatcaa
     1501 attcattctt actattaaat ttgctaaaag acatataagt taaactatct tcagtagaca
     1561 attctccata aacttgaaat aagtcttcaa aatgatctgg atttattttt tctattctaa
     1621 ctgttttccc attaagtgta tcaatcttcg gatttttacc ttccgtaaaa ttttcaacga
     1681 catcacccac tagttgatta aacctattgt atctcattta atttttctgc tttcacaaat
     1741 ttttattgtc gtctatttaa aatattattc ctttaaaata gaataatatg ctctggtttt
     1801 ccactcacct tttggatttt tttacaatat gacgacgttc accttctttt tgcattctta
     1861 ggcattccat aacattttca gattttttat tattagttca aatcgagcat aaattctttc
     1921 caaatctagt tgttcaaatc caattgacat caattttgaa caagctttta tcatataacc
     1981 ttttccacta taatttttat ttaatatata accgaattct gctggaaact cgtctctaat
     2041 ttctaattct ataataccaa tcattttatt tgattctgtt tcaatcatcg catagattcc
     2101 taataatgat ttcatacaga cattcgccaa aatcaatgtg cttcttctgt ggtattggct
     2161 ttatatcgtt gcatatgaaa tacagtttca aaatcagatg aaaattctaa aaactcattt
     2221 acattagata aatcaacaag ctgaagtcgc tacctttcca ttagtactat gattgcacat
     2281 tgctaaatta caatatagtg tatccaaatt attcatttct aatcaatatt ccttccttaa
     2341 ttatctataa accacttcaa taatctccaa aaatacaata ttataagctt taaatttctt
     2401 tgtaagtcta taaaattatt ctaaattatt attatgtaaa tttatactat aaatcagttt
     2461 atctgtccat tttccattct caaatgaaaa atcttttcga gttgtctcat aggttaatcc
     2521 caatcttcta accaactttt ccgatcgttc attcccagga gtaatctgtg cttcaatatg
     2581 atgaaaaccc aatttttcat aagtactatt aagtaatgcc aagattgact caaatgcata
     2641 gccattactc caaaattgat tatgaaaaac atatcccaat tctccccatt gcatatctgc
     2701 tcttgcaagg gtaacaatat ttaacattcc tacgtgctca ccgcttttta gaaaaacacc
     2761 aaatatatat actatgtcac tttcccattc tttcctttgt aaatttacta aatttttaaa
     2821 ccaattaatg tcacacatag acatatcaag tatcccatca tcaaatggag ttaaagatgg
     2881 tccacgattc ataaaactag tataccaact atcataatca ctttcttcta gtggccttat
     2941 aataagtctc tctgattcta aaaaaaattt attcaatttt tatttccttt cttccagagt
     3001 aaataaataa gtgtgtttta attaaaattt ccataatatt acctccttga aaagttatta
     3061 actacattat aaaaaataca ataataaaaa acaaatataa gtaattttag gtaact
//
LOCUS       DATRUK010000107         2962 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.107, whole genome shotgun sequence.
ACCESSION   DATRUK010000107 DATRUK010000000
VERSION     DATRUK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.107"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..272
                     /locus_tag="VBE34_001901"
     CDS             <1..272
                     /locus_tag="VBE34_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome assembly protein"
                     /protein_id="HEO7264567.1"
                     /translation="WDSTSEVKKLIDKTTLSTNEKDRLKLYFEQRAYLETRLNDRYQK
                     FDNSIENQNKELKILTSKIEKIYQKHGITKEVLKTYYAKKTVRAD"
     gene            complement(362..2863)
                     /locus_tag="VBE34_001902"
     CDS             complement(362..2863)
                     /locus_tag="VBE34_001902"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEO7264568.1"
                     /translation="MTFYNHKEIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVAEYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFKLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQVLTASGESISYVPWPSYDESKLVENEIEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVVK"
BASE COUNT          828 a          647 c          628 g          859 t
ORIGIN      
        1 catgggatag cacaagtgaa gtcaaaaaat tgattgataa gacaacccta tccactaacg
       61 aaaaagatag attaaaatta tattttgaac aacgtgctta ccttgagaca aggttgaacg
      121 accgctatca aaaatttgat aactctattg aaaaccaaaa taaagaacta aaaatattaa
      181 cgtcaaaaat agaaaaaatc tatcaaaaac atggtattac aaaagaggta ttaaaaactt
      241 actatgctaa aaaaacagta cgagctgact gatttagcta aaaatagaaa aatcccaaaa
      301 gttcataaac ttttgggatt ttatacgcgt aaatatcact atttctagta aaatctttat
      361 cttatttcac cacaatatta accagtttgt ttggtactgc gatgactttg atgatgtctt
      421 tgcctgcgat ttcggcttgg actttttcgt tagcaagggc aacttcttgt aattcttcgc
      481 gtgaaaggtc tttagcaact acaagtttag ctttgacctt acctttgatt tggacaacga
      541 tttcaatttc gttttcaacc aatttgcttt cgtcataact tggccatggt acgtaagaga
      601 ttgactcacc tgaggctgtc aagacttgcc agagttcctc acccaagtgt ggtgcgaatg
      661 gtgcaatcaa ttgaacaaag cctttggcat agtcgctaaa gagtttgtct tccttgttgg
      721 ctgcattgac aaagaccatg agttgagcga tagctgtgtt gaatttcatt tggtcaactt
      781 gctcagtaac tgccttaacg gtttcgttgt aaaccttgtc aagggctccg ctattttctt
      841 cagtaatctc tttagtcgtg atcaaacggt aaacacgatc caagaactta cgtgagcctt
      901 caagaccttc ttcagaccaa gcgattgaag cgtcaagtgg tcccatgaac atctcgtaaa
      961 cacgaagggt gtcagcaccg tactgttcaa caacatcatc tgggttgaca acgttcttaa
     1021 gtgatttaga catcttagct ggtgcttgtt caagctcttc cccagtttcc acatggaaga
     1081 agctaccatc acgtttttca accttatcag ttgccacaag agcaccacgg ctatcgcggt
     1141 agcttgttcc caaaatcatc ccttggttaa agagtttttg gaatggctct ttggtaggta
     1201 cgacaccgag atcgtaaaga accttgtgcc agaagcgagc gtagagaagg tgaagcacgg
     1261 cgtgctctgc accaccgaca tagatatcaa ctggcaacca ttgtttgagg agttcctcgt
     1321 cagccaattt ttcagtattg tgtggatcga tgtaacggag atagtaccag cttgaacctg
     1381 cccattgtgg catggtgtta gtctcacgac gacctttaac accatcttca cgagtcactt
     1441 ccaaccagtc tgtcaagtta gcaagtgggc tctcaccagt acctgaagga cggatatcct
     1501 tagttactgg aagaaccaat ggcaattcac tctcaggaac cgctgttgat gtaccatctt
     1561 cccagtggat gattgggatt ggctcacccc agtaacgttg acggctgaag agccagtcgc
     1621 ggagacggta agtgactttc tcattaccaa caccttctgc ttcaagccat tcgaccattt
     1681 tggcaatagc tgacgcttta tcgagaccat ctaggaagtc tgagttgatg tgaagaccgt
     1741 cttctgtaaa ggcagcctct tcaacattac caccttcaag aactggaatg atgtctagct
     1801 taaattgttt tgcgaattcc cagtcacgtt catcgtgggc tggaacagcc atgatagcac
     1861 ctgtaccgta gctggcaagg acatagtccg caatccaaac tggaatttct ttaccattaa
     1921 ctgggttaat ggcataggca cctgtccaga cacctgtttt ttctttggca aggtctgtac
     1981 gagcaaggtc tgatttgaga cttgcttgac gtttgtattc ggcaacggct tctgcttggt
     2041 cagctgttgt aatagcatca accaaggcat gctcaggagc aagaactgcg taagttgcac
     2101 caaaaagggt gtcaggacgt gttgtaaaga cagtgaagtc cttatctgta tccttaacct
     2161 tgaaggttac attagcacca gttgatttac caatccagtt gcgttgcatg tctttgattg
     2221 attctggcca gtcaacttct tccaagtctt ctagcaagcg ctctgcatag gcagtgattt
     2281 tgagcatcca ttggcgcatt ggttttctga caactgggta gccaccacgt tctgatgtac
     2341 catctggaag aacctcttcg ttggcgatag ctgttcccaa ttcttcaacc cagttaactg
     2401 gaacttcagc ttcgtaagct agaccttttt cataaagctt ggtaaagatc cactgtgtcc
     2461 acttgtagta gttagggtca gttgtgttaa cttcacgatc ccagtcgtat gagaaaccaa
     2521 gagcattaat ctggcgtttg aagttagcga tgttttcagc tgtaaactca gctgggtcat
     2581 taccagtatc catagcgtac tgctcagcag gaagtccaaa agcatcccaa cccattgggt
     2641 ggaggacatt gtgaccttgg gcacgtttga aacggctgag aatatccgtt gctgtatagc
     2701 cttctgggtg accaacgtgg agaccagctc cagatgggta tgggaacata tctagggcat
     2761 aaaacttagg ttttgatgcg tctgttcctg ttttaaaggt gtgattatct gcccaaaaag
     2821 cttgccattt gggttcgatt tctttgtgat tatagaatgt catcgcactt ctccatgtca
     2881 aaaaagtata gtcctcttat tataacacga aaaacagcaa taaaaaaggt gaaaataccc
     2941 tcaacagcag gttctctccc ca
//
LOCUS       DATRUK010000108         2933 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.108, whole genome shotgun sequence.
ACCESSION   DATRUK010000108 DATRUK010000000
VERSION     DATRUK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.108"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..1179
                     /locus_tag="VBE34_001903"
     CDS             <1..1179
                     /locus_tag="VBE34_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO7264569.1"
                     /translation="KMVLKYSFYYIFISSSLFFIFKNSFTTTRLSFFTFIAMNSILLY
                     LLNSFLKYYRKYSYAKFSRDTKVVLITNKDSLSKMTFRNKYDHNYIAVCILDSSEKDC
                     YDLKHNSLRIINKDALTSELTCLTVDQAFINIPIELFGKYQIQDIINDIEAMGVIVNV
                     NVEALSFDNIGEKRIQTFEGYSVITYSMKFYKYSHLIAKRFLDITGAIIGLLICGIVA
                     IFLVPQIRKDGGPAIFSQNRVGRNGRIFRFYKFRSMRVDAEQIKKDLLVHNQMTGLMF
                     KLEDDPRITKIGKFIRKTSIDELPQFYNVLKGDMSLVGTRPPTVDEYEKYNSTQKRRL
                     SFKPGITGLWQISGRNNITDFDEIVKLDVQYINEWSIWSDIKIILLTLKVVLLGTGAK
                     "
     gene            1203..1652
                     /locus_tag="VBE34_001904"
     CDS             1203..1652
                     /locus_tag="VBE34_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HEO7264570.1"
                     /translation="MKICLVGSSGGHLAHLNLLKPIWEKEDRFWVTFDKEDARSILRE
                     EIVYHCFFPTNRNVKNLVKNTILAFKVLRKERPDVIISSGAAVAVPFFYIGKLFGCKT
                     VYIEVFDRIDKPTLTGKLVYPVTDKFIVQWEEMKKVYPKAINLGGIF"
     gene            1652..2125
                     /locus_tag="VBE34_001905"
     CDS             1652..2125
                     /locus_tag="VBE34_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HEO7264571.1"
                     /translation="MIFVTVGTHEQQFNRLIKEVDRLKGTGAIDQEVFIQTGYSDFEP
                     QNCQWSKFLSYDDMNSYMKEAEIVITHGGPATFMSVISLGKLPVVVPRRKQFGEHIND
                     HQIQFLKKIAHLYPLAWIEDVDGLAEALKRNIATEKYQGNNDMFCHKLEKIIGEI"
     gene            2122..>2933
                     /locus_tag="VBE34_001906"
     CDS             2122..>2933
                     /locus_tag="VBE34_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein CapK"
                     /protein_id="HEO7264572.1"
                     /translation="MRKYLDLDYSLFYALWVLILVPNQWYQFLIITIIVLLLLWKSEF
                     RISISNSSILFLLWLFIYLFAILIRGTQEDITFQRFIAELLKLISTGYALFFYNYYRK
                     ADFNSSVVRNVVKVNYFVLFLITVLYLFFPMLKPTLFGRELFSIEWFPHMRIRLAAYF
                     EYATLIGQFILFSYPILFLKPQKHMENILISLLLTICSYFSGARILLVCMLVLLASLL
                     LDYILFKTNLKLTKKNTFILGMTFLFITACFSYNIWSIIEKIIMYRNQSTIT"
BASE COUNT          982 a          391 c          516 g         1044 t
ORIGIN      
        1 aaaatggtat tgaaatacag cttttactat attttcatat caagttcatt attttttatt
       61 tttaaaaact cttttacaac gacacgactt tcctttttta cttttattgc tatgaattcg
      121 attttattat atctattgaa ttcattttta aaatattatc gaaaatattc ttacgctaag
      181 ttttcacgag ataccaaagt tgttttgata acgaataagg attctttatc aaaaatgacc
      241 tttaggaata aatacgacca taattatatc gctgtctgta tcttggactc ctctgaaaag
      301 gattgttatg atttgaaaca taactcgtta aggataataa acaaagatgc tcttacttca
      361 gagttaacct gcttaactgt tgatcaagct tttattaaca tacccattga attatttggt
      421 aaataccaaa tacaagatat tattaatgac attgaagcaa tgggagtgat tgtcaatgtt
      481 aatgtagagg cacttagctt tgataatata ggagaaaagc gaatccaaac ttttgaagga
      541 tatagtgtta ttacatattc tatgaaattc tataaatata gtcaccttat agcaaaacga
      601 tttttggata tcacgggtgc tattataggt ttgctcatat gtggcattgt ggcaattttt
      661 ctagttccgc aaatcagaaa agatggtgga ccggctatct tttctcaaaa tagagtaggt
      721 cgtaatggta ggatttttag attctataaa ttcagatcaa tgcgagtaga tgcagaacaa
      781 attaagaaag atttattagt tcacaatcaa atgacagggc taatgtttaa gttagaagat
      841 gatcctagaa ttactaaaat aggaaaattt attcgaaaaa caagcataga tgagttgcct
      901 caattctata atgttttaaa aggtgatatg agtttagtag gaacacgccc tcccacagtt
      961 gatgaatatg aaaagtataa ttcaacgcag aagcgacgcc ttagttttaa gccaggaatc
     1021 actggtttgt ggcaaatatc tggtagaaat aatattactg attttgatga aatcgtaaag
     1081 ttagatgttc aatatatcaa tgaatggtct atttggtcag atattaagat tattctccta
     1141 acactaaagg tagttttact cgggacagga gctaagtaaa ggtaaggttt gaaaggaata
     1201 taatgaaaat ttgtctggtt ggttcaagtg gtggtcatct agcacacttg aaccttttga
     1261 aacccatttg ggaaaaagaa gataggtttt gggtaacctt tgataaagaa gatgctagga
     1321 gtattctaag agaagagatt gtatatcatt gcttctttcc aacaaaccgt aatgtcaaaa
     1381 acttggtaaa aaatactatt ctagctttta aggtccttag aaaagaaaga ccagatgtta
     1441 tcatatcatc tggtgccgct gtagcagtac cattctttta tattggtaag ttatttggtt
     1501 gtaagaccgt ttatatagag gttttcgaca ggatagataa accaactttg acaggaaaat
     1561 tagtgtatcc tgtaacagat aaatttattg ttcagtggga agaaatgaaa aaagtttatc
     1621 ctaaggcaat taatttagga ggaatttttt aatgattttt gtcacagtgg ggacacatga
     1681 acagcagttc aaccgtctta ttaaagaagt tgatagatta aaagggacag gtgctattga
     1741 tcaagaagtg ttcattcaaa cgggttactc agacttcgaa cctcagaatt gtcagtggtc
     1801 aaaatttctc tcatatgatg atatgaactc ttacatgaaa gaagctgaga ttgttatcac
     1861 acatggcggc ccagcgacgt ttatgtcagt tatttcttta gggaaattac cagttgttgt
     1921 tcctaggaga aagcagtttg gtgaacatat caatgatcat caaatacaat ttttaaaaaa
     1981 aattgcccac ctgtatccct tggcttggat tgaagatgta gatggacttg cggaagcgtt
     2041 gaaaaggaat atagctacag aaaaatatca gggaaataat gatatgtttt gtcataaatt
     2101 agaaaaaatt ataggtgaaa tatgaggaaa tatctagatt tagattattc tttattttat
     2161 gctctttggg tacttatttt agtaccaaac caatggtatc agtttttaat tattaccatt
     2221 atagttctat tattactttg gaagagtgag tttagaatat ctataagcaa ttcttcaata
     2281 ctatttctgc tttggttatt tatttattta tttgcaatac tcattagagg tactcaagag
     2341 gatataacgt ttcagcgatt tattgctgag ctattaaaac taattagtac aggatatgct
     2401 ttattttttt ataattatta tagaaaagct gattttaata gttcagttgt aaggaatgtg
     2461 gtaaaggtta actattttgt gttgtttctt ataacagttt tatatttatt ttttcctatg
     2521 ctgaagccaa ctttatttgg aagagaattg ttttcaatag agtggtttcc acatatgaga
     2581 ataagacttg cggcatattt tgaatatgct acactaattg gtcagtttat tttattttct
     2641 tatcccatac tttttttgaa accccaaaaa catatggaaa atattttaat atccttactg
     2701 ttgactatat gttcatactt ttctggcgct agaatactat tggtctgtat gttggtttta
     2761 ttagcttcgc ttcttttaga ttatatcctt tttaaaacta atttgaaatt gaccaagaaa
     2821 aacactttta tacttggtat gactttctta tttatcaccg cttgtttttc ttataacata
     2881 tggtcaataa ttgaaaaaat aattatgtac agaaaccaaa gtactatcac tag
//
LOCUS       DATRUK010000109         2726 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.109, whole genome shotgun sequence.
ACCESSION   DATRUK010000109 DATRUK010000000
VERSION     DATRUK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.109"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(77..1459)
                     /locus_tag="VBE34_001907"
     CDS             complement(77..1459)
                     /locus_tag="VBE34_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7264573.1"
                     /translation="MLTTKENFKGNNKLLVGIVLAVLSFWLFAQSILNMGPDVQSSLG
                     ISSGAMDIGVSSTALFSGLFIVVTGGLADKLGRVKFTFIGLCLNIIGSLLIVLANGAV
                     LFIMGRIFQGLAAAFIMPSTMALVKTYYDGKDRQRAVSFWSIGSWGGSGLCSYFGGAV
                     ASTLGWRYVFIFSIIASVVSFLLILGTPESKNVGQKTHFDYLGLIIFIISMLSLNIGI
                     SMAQEHGLMNVIPLSLFTVMLIGFVLFYYVETRKSNSFIDFHLFENRFYLGATISNFL
                     LNAVAGTLIVINTYMQQGRQLTPKVAGEMSLGYLVCVLIAIRVGEKILQRFGARKPML
                     LGAMSTFVGIFLMTLVNIQGPLYLVLVFVGYALFGTGLGIYATPSTDTAISSIPNEKV
                     GSASGIYKMASSLGGAIGVATSIAIYHAFSGNADFHKAALCGLILNLVFCSLSILSIL
                     FVIPKKIEDK"
     gene            complement(1467..2681)
                     /locus_tag="VBE34_001908"
     CDS             complement(1467..2681)
                     /locus_tag="VBE34_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO7264574.1"
                     /translation="MANASLRHQLFEKLDQKCDQMVAIRRYLHENPELSFKETKTAAY
                     ISDFYKGKDCHVQTQFGGMNGVVVDIYGDKATDKPIKHIALRADFDALPIQEETGLSF
                     ASKTAGVMHACGHDAHTAYLLILAESLIELKSEFSGHIRILHQPAEEVPPGGAKAMIE
                     AGCLDGIDAVLGIHVMSTMEEGTVQYHAGPIQTGRATFKVILQGKGGHGSMPHRANDT
                     IVAASSFVMAAQTIVSRRVNPFDTAVVTIGSFDGKGSANVIKDSVTLEGDVRVMSEET
                     RGVVEEEFKRILDGIAQTYGVSYQLDYQNDYPVLVNNSEVTQKVANSLKSVAIKEILD
                     VIDCDPQTPSEDFAYYAQTIPACFFYVGAHEEGQPYYPHHHPKFQIAESSLMVSAKSM
                     ATAALAMLVEGE"
BASE COUNT          929 a          575 c          421 g          801 t
ORIGIN      
        1 tattttaaat ttctttccca attcctttta cattattaca acaatacttg agattttgaa
       61 tctcaagtat tgttatttat ttatcctcaa ttttcttagg gataacaaaa agaattgata
      121 agatcgatag actacaaaaa actaggttta aaattaatcc acacaaagct gctttgtgaa
      181 aatctgcatt accagaaaaa gcatgataaa tcgcaattga agttgctaca ccaattgcac
      241 cacctagtga acttgccatt ttgtaaattc cagatgcaga acctactttt tcattaggaa
      301 tactagaaat agctgtatct gttgatggtg tcgcatagat ccccaagcct gttccaaata
      361 aagcataacc aacaaagact aacacaagat acaaaggtcc ttgaatattg accaaagtca
      421 ttaaaaatat cccaacaaat gtactcatag ctcccaaaag cattggctta cgggcaccaa
      481 aacgttgtaa aattttctca ccaactcgga tagcaattag tacacaaacc aaatagccta
      541 aagacatttc acctgcaacc tttggtgtta gctggcgacc ttgttgcatg taagtattaa
      601 taacaatcag agttcctgca actgcattca gtaagaaatt tgatattgtc gcacctaagt
      661 aaaatcgatt ttcaaacaga tgaaagtcaa taaaactatt agatttacgt gtttcaacat
      721 aataaaacag aacaaatcca ataagcatca ctgtaaaaag actcaatgga ataacgttca
      781 tcaatccatg ttcctgagcc atagatatcc caatatttaa cgataacatt gaaataataa
      841 aaataattaa accaaggtaa tcaaagtgag ttttttgtcc aacattttta ctttctgggg
      901 ttcctaaaat taatagaaaa ctaactactg atgcgatgat tgaaaagata aagacataac
      961 gccaacctag agtacttgcc accgctccac caaaataaga acatagacct gagccacccc
     1021 acgatccgat tgaccagaaa ctaactgcac gttgtctatc tttaccatca tagtacgttt
     1081 taaccaaggc cattgttgat ggcataataa aagctgctgc caatccttga aaaatacgtc
     1141 ccataataaa tagcactgct ccattagcta aaacaattag tagagaacca ataatattta
     1201 agcaaagacc aataaaagta aatttaactc gaccaagttt atctgctaaa ccaccagtta
     1261 caacaataaa taaacctgaa aacaatgccg tacttgagac accaatatcc attgcaccag
     1321 atgaaatacc taatgatgac tgaacatctg gtcccatgtt taaaatagac tgtgcaaata
     1381 accaaaatga tagtacagcg agtacaattc ccaccaataa tttgttattt cctttgaaat
     1441 tttcctttgt cgttaacata tgttatctat tctccttcta caagcatagc aagcgctgct
     1501 gttgccattg atttagcaga taccatcaat gatgattctg caatttggaa ttttggatga
     1561 tgatgtgggt aatatggttg cccctcttca tgtgctccaa catagaagaa gcaagctggg
     1621 attgtttgag cataataggc aaaatcttca gacggcgttt gagggtcgca atcaatgaca
     1681 tctaaaattt cttttatagc aacagatttt aaactatttg caactttctg tgtcacttca
     1741 ctgttattaa ctaaaacagg ataatcattc tgatagtcta actgataact aacaccataa
     1801 gtttgtgcta ttccatctag aatacgctta aattcctcct caacaacacc tcgtgtctct
     1861 tcagacatca cgcgcacatc accttctaag gttacactat ctttgatcac attagcagaa
     1921 cctttaccat caaaagatcc gatagtaact actgctgtgt caaatggatt aactcgacga
     1981 ctgacaatcg tttgcgctgc cataacaaaa ctagaagctg caacaattgt atcatttgca
     2041 cgatgtggca tggaaccatg tccaccctta ccttgcaaaa taactttaaa agtggcacgc
     2101 cctgtttgaa tgggacctgc gtgatattgt accgttccct cttccatcgt tgacataaca
     2161 tggataccaa ggacagcatc aatgccatct aaacatcctg cttcaatcat tgcttttgct
     2221 ccgcctggtg gcacctcttc agccggttga tgtaagatac ggatatgacc tgaaaattct
     2281 gattttaatt caatcaagct ctcagccaaa atcaataaat aagctgtatg ggcatcatgt
     2341 ccacaagcat gcatgacgcc cgctgttttt gaagcaaaag aaagccctgt ctcttcctga
     2401 attggaagag catcaaagtc ggctctcaaa gcaatgtgtt tgatgggctt atctgtcgct
     2461 ttatcaccat agatatcaac tacgacgcca ttcataccac caaattgtgt ctgaacatga
     2521 cagtccttac ctttataaaa atctgaaata taagctgctg tttttgtctc tttaaatgat
     2581 aattcgggat tttcatgtaa atagcgacga atagcaacca tttgatcaca tttttggtca
     2641 agtttttcaa ataattgatg tcgcaaagat gcattggcca tttgctttcc tccttaaaat
     2701 atgaacagtt aaaataaggc tattat
//
LOCUS       DATRUK010000110         2598 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.110, whole genome shotgun sequence.
ACCESSION   DATRUK010000110 DATRUK010000000
VERSION     DATRUK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.110"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            344..841
                     /gene="ybeY"
                     /locus_tag="VBE34_001909"
     CDS             344..841
                     /gene="ybeY"
                     /locus_tag="VBE34_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEO7264575.1"
                     /translation="MYVEMIDETGQVSEDIKKQTLDLLEFAAQKTGKENKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPEVDISFDEEDLAENPELAEMLEDFDSYIGEL
                     FISIDKAKEQAEEYGHSYEREMGFLAVHGFLHINGYDHYTPEEEKEMFSLQEEILTAY
                     GLKRQ"
     gene            822..1220
                     /locus_tag="VBE34_001910"
     CDS             822..1220
                     /locus_tag="VBE34_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEO7264576.1"
                     /translation="MDLNDNNQKKWKNRTLTSSMEFAVTGIFTAFKEERNMRKHLVSA
                     ILVILAGLTFQVSMVEWLFLLLSIFLVITFEIINSAIENVVDLASNYHFSMLAKNAKD
                     MAAGAVLVVSLFAVLVGLIIFIPKILALLL"
     gene            1262..2161
                     /gene="era"
                     /locus_tag="VBE34_001911"
     CDS             1262..2161
                     /gene="era"
                     /locus_tag="VBE34_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEO7264577.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGD
                     DMIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALQGNNVPT
                     LIKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            2398..>2598
                     /locus_tag="VBE34_001912"
     CDS             2398..>2598
                     /locus_tag="VBE34_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264578.1"
                     /translation="MHNILRFLGIVIISAVILFSIGSFYDLSLMKNILLICWSFLFDL
                     LVFVFKQRQTTEVLTWYQVVKQF"
BASE COUNT          871 a          351 c          519 g          857 t
ORIGIN      
        1 gttatagcat ttgtagcttt gtttatttag gaattagttt tacgtaagtt aggcgctcta
       61 aagtttgtat tttgaagtaa atactaattc ataagtcaga attttattga aatggattat
      121 aagtgtgtaa tagtgattat tagctttctc tccctttttt aatactctta tggtataatg
      181 atgtgaaaga atagcttgaa ggaaggaaga gaacgcactt ttattttgag aactagtttt
      241 agataaaagt gaaaaactta ggaaagtaga tgtgtcgatt atttagaatt atgcattaca
      301 ctttatcttt tctaaacgtt ttgggtcgtt cttactatat aagatgtacg ttgaaatgat
      361 tgatgaaact ggacaagtat cagaagatat caaaaagcaa actcttgatt tgttagagtt
      421 tgcagcacaa aaaacaggta aagagaataa agaaatggct gtaacgtttg tcactaatga
      481 acgtagccat gaattgaatt tagagtatcg agataccgat cgtccgactg atgttatcag
      541 tttagaatat aagcctgagg ttgatatttc ttttgatgaa gaagatctgg ctgaaaatcc
      601 tgaacttgct gagatgctag aagattttga ttcttatatt ggtgaacttt ttatttctat
      661 tgataaagct aaagaacaag cagaagagta cggtcactct tatgaacgtg aaatgggttt
      721 tttagctgta catggttttt tgcatattaa cggttatgat cattatacac ccgaagaaga
      781 aaaagagatg ttcagcttac aggaagaaat tttaactgct tatggactta aacgacaata
      841 atcaaaaaaa atggaaaaat cgcaccttaa catcaagcat ggaatttgct gttacaggga
      901 tttttacagc ttttaaagaa gaaagaaata tgcgtaagca tttggtttct gccattttag
      961 taatacttgc tggtcttact tttcaagtat ctatggtgga gtggttattt cttttattga
     1021 gtattttttt ggtaattact tttgaaatta tcaattcagc tattgaaaat gtagttgatt
     1081 tggcaagtaa ttaccatttc tcgatgctgg ctaaaaatgc taaagatatg gcagcaggag
     1141 cggttttagt tgtttcctta tttgccgtat tggtgggatt gattattttt atacccaaga
     1201 tacttgcttt acttttatga tgagaagtat tacgaataaa ttgaatggag aaagaaaaga
     1261 aatgacattt aagtcaggat ttgtagcaat tttaggtcgt ccaaatgttg gaaaatcaac
     1321 cttcctaaac catgttatgg gacaaaaaat tgctatcatg agtgataaag ctcaaacaac
     1381 acgtaataaa attatgggga tttatacaac agaaactgag caaattgttt tcattgatac
     1441 cccaggaatt cataaaccaa agaccgccct tggtgatttt atggttgaat ctgcttatag
     1501 tactttacgt gaagttgaaa cagttctttt catggttcct gccgatgaaa agcgaggcaa
     1561 aggcgatgat atgattattg aacgtcttaa ggctgccaag attcctgtta ttttagtaat
     1621 caacaaaatt gataaggttc atccagacca acttttggaa cagattgatg acttccgtag
     1681 tcaaatggat tttaaagaag tagtgccaat ttctgccctt caaggaaata atgtgccaac
     1741 cctaatcaag ttattgactg ataatcttga agaaggtttc caatatttcc cagaagatca
     1801 gattactgat catccggaac gatttttagt atcagaaatg gttcgtgaga aggtacttca
     1861 tttaacgcaa caagaggttc ctcactcagt agcggttgtt gtcgaatcca tgaaacgtga
     1921 tgaagaaact gataaagtgc atattcgtgc aactatcatg gttgaacgtg atagtcaaaa
     1981 aggcattatc attggaaaac aaggtgctat gctcaagaaa ataggaaaaa tggcgcgccg
     2041 tgatattgaa cttatgttag gtgataaagt ttacctagag acttgggtaa aagtcaagaa
     2101 aaactggcgt gataaaaagc ttgatttagc tgattttggc tataatgaaa aagagtatta
     2161 atgagtttat tttgagatcc gcaagttagt ggggaagagt tagataagtt caccgtgaga
     2221 cttgattcgc acagaaaaag caattcacga ggataaaacc aactagtcat aattttgaga
     2281 ttatattcac aaattaatta aagggagcca aacatgcttg ttggcccctt cttaaataaa
     2341 ggagagcaca tgactcaaga ttatatttgc tacctataaa gtagaggagg tgaggatatg
     2401 cataatatat taagattttt aggcattgtt attatttctg cagtgattct atttagtata
     2461 ggtagtttct acgatttatc attaatgaaa aatatactac ttatctgttg gagttttctc
     2521 tttgatctat tggtttttgt cttcaaacaa agacaaacga cagaagtact cacatggtat
     2581 caagttgtta agcaattt
//
LOCUS       DATRUK010000111         2571 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.111, whole genome shotgun sequence.
ACCESSION   DATRUK010000111 DATRUK010000000
VERSION     DATRUK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.111"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            204..680
                     /locus_tag="VBE34_001913"
     CDS             204..680
                     /locus_tag="VBE34_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264579.1"
                     /translation="MYIIPAGYSPERLDSEVLTAKIEDDEALLRKIQDLKDHLDKNTK
                     ILAYAFDGLEVRVKARLRKNRKKLKNLIEFNAKSTSVFNELKDYSNAVSSGLTFVSKG
                     FSSFNGQFSSASNLDWQKTIKTGMRKREEDAKQLRKKALEDSNKLRKIASKRGYFE"
     gene            936..1273
                     /locus_tag="VBE34_001914"
                     /pseudo
     CDS             936..1273
                     /locus_tag="VBE34_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1667..2047
                     /locus_tag="VBE34_001915"
     CDS             1667..2047
                     /locus_tag="VBE34_001915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264580.1"
                     /translation="MVFVSDGSPVDANTLYFGKGTPIFMIMNVTIFIISTLIYSYYYL
                     PKNQGKVWKFNQWNNEKSREQQYISNFIVTFGLILLVTPFLKYGIYNSFGVLISLLFT
                     ATLPSLIVDSLYAAFYVHKHPEYE"
     gene            2338..2568
                     /locus_tag="VBE34_001916"
     CDS             2338..2568
                     /locus_tag="VBE34_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001902384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264581.1"
                     /translation="MKQSNHRYHGPLIEFEIEYSYIKIFPWIGLFISCIGLYASNFGD
                     PLGFIKFIFSVCIFVNLVSIIISLFLKKYLIF"
BASE COUNT          924 a          322 c          460 g          865 t
ORIGIN      
        1 tggttgtgtg cgatagccac gctatactat taagttatta caaactatga attacacctc
       61 aaatgttgga gataaaacct atcgtttggg gtgttttttg gtaaaataga atgaaaagag
      121 ttactctgtg tcattactaa gttaaaaaat tatactaact taattattaa cgttttattg
      181 actgagttac tcttttttaa aaaatgtata tcattccagc tggttactca cctgaacgtt
      241 tagactcaga agtccttaca gcaaagattg aagatgatga agccttattg cggaaaattc
      301 aagaccttaa agatcattta gataaaaata ccaaaatctt ggcttacgct tttgatggct
      361 tggaagtcag agtgaaagct agactaagaa aaaatcgtaa gaaactcaaa aaccttatag
      421 aattcaatgc caaatctacc tcagtcttta atgaattgaa ggattattca aatgctgtat
      481 cgtctggttt aacctttgta tctaaaggct ttagtagttt caatggccag ttctcttcag
      541 cctcaaatct tgattggcaa aagactatta agacaggaat gagaaaacga gaagaagatg
      601 ctaaacagtt gagaaaaaaa gctttagaag attcaaataa gctcagaaaa attgcatcaa
      661 aaaggggtta ctttgaataa tgcagttgaa tttgctaaag aacagaacaa acccctaaaa
      721 ataaaaagat aaccatagat aaatatggtc gtgtaaaaat ggataaaact tttctttatc
      781 gaaaagctaa agatggaaaa tctgcatata tttattctga agggagtgat caatataaag
      841 aactaactgg gaaagaattt gatcctaaaa ctacaaatat tatgaaaggt agaaaattag
      901 ggacagatgg taagatatca attgattttg gtgagattag taactcagga aaaaatgcag
      961 ctattaagtc ccttaaaaat gaaagtcctt gaattaaatc ccaaaaatgc ttttaaagga
     1021 tggaatgagt taagcaaaat agctaaaact gggaaagtac taggatggac aggtaaaatt
     1081 gctaatattt acatgaatgg aaaaaaagat ctttttgatg ataaaacatc ttcaatagat
     1141 caaaaaatca aaaactttgc tataggttca ggtgtagatg cattgtccgg tgcagcaata
     1201 gggactttgg taggaggacc tgtgggaacc gttgttggag caggtgcagg gctggcattt
     1261 gattttgctt tgaattttaa agttaaggag ttgggtaatg aatcaattac aggttttgct
     1321 aaaaaaggtt taaaaaatct tgcaggagga ttaggatttt gaaaaaaagt ataaatgtaa
     1381 gtaggtatca tggaccttta attgaatttg aggttgatgc aagttattta agaatttttt
     1441 catatatcgg tttaccttta tcattgtttt ggtattttgt tactcataac agtgatgact
     1501 ttggaatatt aaaatcgata tttttaattt gtattatagt caatttaatc gggctcatat
     1561 taggattaat acaaaagttt actaaaaaac ttatcaaatc tctatcattt tttgtacttt
     1621 cttagcgttt ctttcgattt tcttagggtt agatacaata tgtttaatgg tatttgtttc
     1681 ggatgggtcc cctgttgatg cgaatacgtt gtattttggt aagggtactc caatttttat
     1741 gataatgaac gttacgatat ttatcatttc tactttgatc tattcctact attatttacc
     1801 aaaaaatcaa gggaaagttt ggaaatttaa tcaatggaat aacgaaaaga gtcgagaaca
     1861 acaatacata agtaatttta ttgttacatt tggtctgata ttgttagtta ctccattttt
     1921 aaagtatggt atctataaca gttttggagt tttaatcagt ttactattta cagcaacctt
     1981 accatcatta attgttgatt cactttatgc ggcattttat gtccataagc atcctgagta
     2041 tgaataatca acttataaag aacatcgaaa acacatgata ccttttataa gacagaaact
     2101 ataaattcag ataaaacatc ttcgcttgat aagtcagaga ctttaaagat agaggataac
     2161 aagctatttt agacaaaaaa ttagaaattt cacaatagat tctagagtag atactttatt
     2221 aggtgctgga aggttattgg gcacttgatt gtgattggac tggacaaaac aaaccagatc
     2281 aatcagtaac tggttttgca aaaaagaatt aaaagattta tttggagggg aaaatttttg
     2341 aaacagagca atcatagata ccatggaccg ttaattgagt ttgaaataga atattcatat
     2401 attaaaattt ttccatggat tggactattt atatcttgca tagggttata tgcaagtaat
     2461 tttggtgatc ctttaggttt tataaaattc atattttctg tatgcatttt tgttaatttg
     2521 gtctcaataa ttatttcgct ttttttgaaa aaatatctaa ttttttaaaa a
//
LOCUS       DATRUK010000112         2541 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.112, whole genome shotgun sequence.
ACCESSION   DATRUK010000112 DATRUK010000000
VERSION     DATRUK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.112"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(212..502)
                     /locus_tag="VBE34_001917"
     CDS             complement(212..502)
                     /locus_tag="VBE34_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEO7264582.1"
                     /translation="MSQIKLTPEELRISAQKYTTGSQSITDVLTVLTQEQAVIDENWD
                     GTAFDSFEAQFNELSPKITQFAQLLEDINQQLLKVADVVEQTDSDIASQINK"
     gene            complement(957..1349)
                     /gene="rpsI"
                     /locus_tag="VBE34_001918"
     CDS             complement(957..1349)
                     /gene="rpsI"
                     /locus_tag="VBE34_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEO7264583.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITINKKDVEEYIPHADL
                     RLVINQPFAVTSTQGSYDVFVNVVGGGYAGQSGAIRHGISRALLEVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1370..1816)
                     /gene="rplM"
                     /locus_tag="VBE34_001919"
     CDS             complement(1370..1816)
                     /gene="rplM"
                     /locus_tag="VBE34_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEO7264584.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKASDKIYYTHSMYPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(2038..2160)
                     /locus_tag="VBE34_001920"
     CDS             complement(2038..2160)
                     /locus_tag="VBE34_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002278327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7264585.1"
                     /translation="MKNLRLKTARASMDLLQQSLAEKVGVSCQTIAAIEKGGYN"
BASE COUNT          790 a          461 c          409 g          881 t
ORIGIN      
        1 tgctttgact agactaactt catattctcg attccgtttt agttcttctt ttctttgttg
       61 ttctttcata taaaatcctc cgctaaaaac aagtatgaat aatgcaacta ttagtagtat
      121 tatttgggta ctctttttca tttttactcc tttatgaaac aattacatca tctagctata
      181 atatatcaac ttatgaattt gaataagcca attatttatt aatttgtgag gcaatatctg
      241 agtctgtttg ttcgacaaca tccgcaactt tcaataattg ttgatttata tcttctaata
      301 attgtgcaaa ttgtgtgatt tttggagata attcattgaa ttgggcttca aagctatcaa
      361 atgctgtacc atcccaattt tcatcaataa cagcttgttc ttgagtcaaa actgttaaca
      421 catctgtaat tgattgtgat cctgttgtat atttttgtgc tgaaatacgg agttcttcag
      481 gtgttagttt aatttgtgac atcatttgct ccctattttt ttaactgcag tatagcatat
      541 ttagatgttc cttacacaga taaaactcat aataggttaa tctattaaaa atacaaacag
      601 cgatttatat tacatctact ttactttcaa aacggaaagg ttatcaaaag attttaaaaa
      661 aacaccctac aatatcttgc aaagtgcttt gaaacgttga tataacagta tattaacatt
      721 ttgagaattg tgatgcttta cgggcttttt taagacctgg ttttctctga caactgtaat
      781 atatggtgtt atatagtata aactagctaa tactcatcaa tttacatcca aaaagtgtac
      841 tatgtattgc aatatattcg cgaaggggtt atcaggttat caaaagtaaa attgacaata
      901 aaaaaacacc ttgcaattgc ttgcaaagtg ctttgaaacg ttgatataac agtatattaa
      961 cgttttgaaa attgtgatgc tttacgtgct tttttaagac ctggtttttt acgttcaacc
     1021 atacgtgcgt cacgtgtaag aagtccagca cgtttcaatg aatcgcggaa atctgggtca
     1081 acttctaaaa gtgcacgtga gataccatga cggatcgcac ctgattgacc tgcatatcca
     1141 ccacctacaa cgttaacaaa aacatcgtat gaaccttgag ttgaagtaac tgcgaaaggt
     1201 tggttgataa caagacgaag gtctgcgtgt gggatgtact cttctacatc ttttttattg
     1261 attgtaattt taccagtacc tggaaccaaa cgaacgcgtg caacagcgtt tttacggcga
     1321 ccagtacctg catattgtgc ttgtgccatt atataatgct cctttcctct tagataagtc
     1381 ctgagatatc aagtacttct ggttgttgtg cagcatgtgt atgctcaccg ccaacaaata
     1441 ctttcaattt cataccttgt gcacgtccaa gagtgttatg tggaagcatg cctttaactg
     1501 atttttcaat caaacgaaca gcatttttag aacgaagttc acctgctgag atttgtttca
     1561 aaccacctgg atacattgag tgagtgtagt agattttatc tgatgctttt ttaccagtta
     1621 atttaacttt ttcagcattg ataacaatca caaagtcacc tgtatcagtg tgtggtgtga
     1681 aagttggttt gttttttccg cgaagtacgc tagcaactac tgcagaaaga cgtccaagag
     1741 gtacatctgt tgcgtcaaca acgtaccatt tgcgttcaac ttggcctggt ttagccatga
     1801 aagttgtttt gttcatgatt tctcctatac gaattcgttt ttgtctacag gtgtggatgt
     1861 tccggtccac gagagtattt gaaaggttcc ggggccttac aaatggggta aacaataccg
     1921 cttataatca tatcaaaata aggtagcatt cgtcaagtta tttcttttat tttatcaatt
     1981 ttcatcccaa aataaatcat ctacagtttt attcaaagct tgacacaatt taatcgctta
     2041 attatagcct cctttttcaa tggcggctat tgtctgacat gatacaccga ccttttcagc
     2101 tagagactgt tgtaataaat ccatactagc tctagctgtc tttaagcgta aatttttcat
     2161 attactatct gtccttatac ttaaggtagt atactgaagt tcccatagct atcaatgata
     2221 ttcctataac tgctaaaatt gcatcatttc cccttgaaaa ataagtagca atatcaactt
     2281 cctagtaaaa gcatcaataa aatagttatt cctatgaggg caaatgcgcc agcaacaaaa
     2341 acattttcca aaatctttca tcaacaaacg gagagactct ttttagatta gaagtcgtaa
     2401 caaaatagaa ataccactaa ataaagttaa tattattata aaatcaggtg acacgcttcc
     2461 ctttattagc tttccaaaaa cactttcaag ccataagata aaaaacatcg tctaaaaaca
     2521 tttttgataa ctttgtaaaa c
//
LOCUS       DATRUK010000113         2534 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.113, whole genome shotgun sequence.
ACCESSION   DATRUK010000113 DATRUK010000000
VERSION     DATRUK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.113"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            111..956
                     /locus_tag="VBE34_001921"
     CDS             111..956
                     /locus_tag="VBE34_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HEO7264586.1"
                     /translation="MLSVGLVLEGGGMRGLYTAGVLDAFLDAGIKIDGIVSVSAGALF
                     GVNFVSRQRERALRYNKKYLSHPEYMSLRSWLRTGNFVNKDFTYYEVPMKLDVFDDEA
                     FKKSSIDFYVVATEMTSGKPEYFKIDSVFEQMEILRASSALPVVSKMVDWQGKKYLDG
                     GLSDSIPVDFARGLGFDKLIVVMTRPLNYQKKPSSGRLYKTLYRKYPNFVKTASNRYQ
                     QYNNSLEKVMSLEKTGDLFAIRPSKSLVIGRLEKNPDKLDSIYQLGMKYAKSVMPELN
                     SYLMK"
     gene            complement(999..2246)
                     /locus_tag="VBE34_001922"
     CDS             complement(999..2246)
                     /locus_tag="VBE34_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7264587.1"
                     /translation="MSPLNINKNNWRALIAAIVASGTDDLNIMFLAFSMSTIITDLHL
                     SAAQAGWIGTITNLGMLVGGLIFGLLADRYNKFKVFKWTILIFSIATGLVFFTTNLSY
                     LYIMRFIAGIGVGGEYGIAIAIMAGIVPANKMGRISSLNGIAGQVGSISSALLAGWLA
                     PALGWRGLFLFGLLPIVLVLWMQFAIDDKDILDQYNTDADDEPLDISIKALFDTPVLA
                     TQSLALMVMTTVQIAGYFGMMNWLPTIIQTNLNVSVKNSSLWMIATIFGMCLGMLVFG
                     QLLDKFGPRLVYGCFLLSSAICVYLFQFATTMPSMIIGGAVVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNLILNVGRAIGGFSSVIIGMILDVSNVSMVMLFLASLYIVSFLSML
                     SIKQLKRQKYHTNLTQLDVKPTD"
BASE COUNT          853 a          410 c          511 g          760 t
ORIGIN      
        1 attattaaat taattatgga gtttatatag tacaacatat tctttttcag aaaaaacagc
       61 tatattttgc tatactttaa tcaaatatca atattagaaa gtgattgcct atgttgtctg
      121 ttggtttagt tttagagggt ggcggaatga gaggtcttta tactgctgga gttttagatg
      181 cttttctaga tgcaggaata aaaatagatg gtatcgtatc tgtctctgcg ggtgcattgt
      241 ttggtgttaa ttttgtatct agacaacgag agagggcttt gcgatacaat aaaaagtatt
      301 tatcccaccc tgaatatatg agtctaagat catggcttcg aacagggaat tttgttaata
      361 aagatttcac ctattatgaa gttcctatga aattggatgt atttgacgat gaagcattta
      421 aaaaatcaag tattgatttt tacgtagttg ctacagagat gacatctggt aaacctgaat
      481 attttaaaat tgatagtgtt tttgaacaaa tggaaatttt acgtgctagt tcagcattac
      541 cagtagtctc aaagatggtt gattggcagg ggaaaaagta cttagatggt ggtttatctg
      601 atagtattcc cgttgatttt gcccgtggtt taggatttga caagttgatt gttgtgatga
      661 ctaggccgct caattatcag aaaaagcctt caagtggacg attgtataaa actctgtata
      721 ggaaatatcc taattttgta aagacagcct cgaatcggta ccaacagtat aataatagtc
      781 ttgaaaaggt catgagcctt gaaaaaacag gcgatctatt tgcaattaga ccgagtaaga
      841 gcttggttat tggccgctta gagaagaatc cggataaact tgatagtatt tatcagcttg
      901 gtatgaaata tgctaaaagt gtgatgcctg agctgaatag ttatctaatg aaataaagaa
      961 aacagtattt ctcaattgag gaatactgtt ttgtaatatc agtcagttgg tttgacatct
     1021 aattgtgtta aattagtgtg atatttttga cgttttaatt gcttaatgct tagcattgat
     1081 aagaaactaa cgatatagag acttgctaag aaaagcatga ccatagagac atttgaaacg
     1141 tctagaatca ttccgatgat aacagacgaa aagccaccta ttgcacgacc aacatttaag
     1201 ataagattgt tagctgttga acgaatatga tgtggataga gacgtgtaat catggcacca
     1261 tagcctgcaa acataccatt aacaaagaag ccgacaactg ccccgcctat aatcatagaa
     1321 ggcattgttg ttgcaaattg aaagagatag acacaaattg cagatgatag aaggaaacac
     1381 ccgtatacta aacgcggtcc aaatttatct aacaattgac caaatactag catgccaaga
     1441 cacattccaa aaatggttgc aatcatccat aatgatgaat tcttgacaga aacgtttaag
     1501 ttggtttgga taatagttgg taaccagttc atcatgccaa agtatccggc aatctgaaca
     1561 gttgtcataa ccattagtgc taggctctgt gtggctaata caggagtatc aaataaagct
     1621 tttatactga tatctagagg ttcatcatct gcgtctgtat tatattgatc taagatatct
     1681 ttatcatcaa tagcaaattg catccatagg acgagaacta taggaagaag gccaaagaga
     1741 aaaagtcccc gccaaccgag tgctggtgct agccatcctg ctaagagtgc tgagctaata
     1801 gatccgactt gaccagcgat accatttagt gaagaaatcc ggcccatttt attagctggg
     1861 acaattcctg ccataatggc aatagcgata ccatattcgc caccaacgcc aattcctgca
     1921 atgaagcgca tgatatagag atatgataaa ttagtagtga agaaaactaa accagttgcg
     1981 attgaaaaga ttagaatcgt ccatttgaaa actttgaatt tattgtagcg atcagctaat
     2041 aaaccaaaga ttagtcctcc cacgagcatt cccaagttgg taattgtccc aatccaacca
     2101 gcctgtgccg cagataaatg aagatcagta atgatagttg acatagaaaa tgcaaggaac
     2161 atgatattta aatcatcggt tccggatgcg acaatggcag cgattagggc gcgccaatta
     2221 ttcttattga tatttaatgg tgacataagt ctcctccttt aatgatggct cacagaccac
     2281 cgttattaaa tactgtcaat agaaaaaatt ctaaagcaga gatgtcatag ctgcttcaac
     2341 tcattataat gtaaaagaaa aaactcaagc tgctaccctt gagttctata aacgctcgca
     2401 aaaatagtag gatttctgta tagaatccta ttttggtcgc ttatatgtct cacaggacac
     2461 ggttaaagca ttatgtcatt tggaaatagt atagagtaag tttagtttat tgtcaaggat
     2521 aagtggggag agaa
//
LOCUS       DATRUK010000114         2303 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.115, whole genome shotgun sequence.
ACCESSION   DATRUK010000114 DATRUK010000000
VERSION     DATRUK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.115"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..416
                     /locus_tag="VBE34_001923"
     CDS             <1..416
                     /locus_tag="VBE34_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264588.1"
                     /translation="QNIKGDNYESGSSRNDVSGSGNIVGTNIDNGSQVSLSGDISNGS
                     TIIKDSNINNADIHKSVEIPWTDMVEQLNLIINTQLSTADKAIYSELLEISEAEDERK
                     LKNFVNKMSSFFKNDYVQNTITGVLGNVISSILMK"
     gene            434..1732
                     /gene="purB"
                     /locus_tag="VBE34_001924"
     CDS             434..1732
                     /gene="purB"
                     /locus_tag="VBE34_001924"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEO7264589.1"
                     /translation="MISRYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEKYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEEIDELFNPIYYTKRVDDIFERLGLEK"
     gene            1885..>2303
                     /locus_tag="VBE34_001925"
     CDS             1885..>2303
                     /locus_tag="VBE34_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7264590.1"
                     /translation="MLKHFGSKVRNLRVTRNITREDFCGDETELSVRQLARIESGQSI
                     PNLTKAHYIAKQLNVKLDILTGGESLELPKRYKELKYLILRIPTYADAERLKLRECQF
                     DHIFEEFYDNLPEDECLAIDSLQAKFEVYQTGDINFGV"
BASE COUNT          761 a          371 c          507 g          664 t
ORIGIN      
        1 accaaaatat aaagggcgat aattatgaat ctggatctag tcgaaatgat gtctcaggta
       61 gtggtaatat agttggaaca aatattgata acggtagtca agttagttta tctggggata
      121 tatcaaatgg ttctactatc ataaaagatt ctaatattaa taatgccgat atacataaaa
      181 gtgtggaaat tccatggact gatatggttg agcagttaaa tttaattatt aacactcagc
      241 tatctactgc ggataaagcg atatattcag aattgttgga aattagtgag gcagaggatg
      301 aaagaaaatt gaaaaatttt gtcaataaaa tgtctagttt cttcaaaaat gattatgttc
      361 agaataccat aactggggtg ttaggcaatg tcatttctag tattttaatg aaataagtta
      421 aaggagacaa acaatgattt cacgttattc acgccctgag atggcggcaa tttggacaga
      481 ggaaaataaa taccgtgctt ggttggaggt cgagattttg gctgacgagg catgggctga
      541 gttgggtgag attcctaaag aggatgtggc taagattcgc gagaaggcgg attttgacat
      601 tgaccgcatt cttgagattg agcaggacac gcgtcacgat gtggtggctt tcactcgtgc
      661 ggtttctgag acgcttggtg aggagcgcaa gtgggtgcac tacggtttga cgtcgactga
      721 cgtggtggac actgcctacg gttacctcta caagcaggct aacgacatta tccgtcgtga
      781 tcttgagaat ttcacaaaca tcgtagctga caaggctaag gagcacaagt tcaccatcat
      841 gatgggtcgt acccacggtg ttcacgccga gccaacgact ttcggtctca agttggcgac
      901 ttggtacagc gagatgaaac gtaatattga gcgttttgaa catgctgccg caggtgtgga
      961 agctggtaag atttcaggtg ccgttggtaa ctttgccaac attcctccat ttgtggaaaa
     1021 atatgtctgt gacaaattgg gcatccgtcc gcaagaaatt tcaacacagg ttcttccacg
     1081 tgacctccac gcagaatatt ttgcagtact tgcaagcatc gcaacatcta tcgaacgtat
     1141 ggcgacagag attcgtggtc tgcaaaaatc agaacaacgt gaagttgaag aattctttgc
     1201 caaaggtcag aaaggtagct ctgctatgcc tcacaaacgc aacccaatcg gttcagagaa
     1261 catgacaggg ctagcgcgcg tgattcgtgg tcacatggtg acagcttatg agaacgtgtc
     1321 actttggcat gagcgtgaca tttcgcactc atcagctgag cgtatcatca caccagacac
     1381 aactatcttg attgactaca tgctcaaccg ttttggcaat atcgttaaga acttgactgt
     1441 cttcccggaa aatatgatgc gcaatatgga atcaactttt ggtttgattt atagtcaacg
     1501 tgttatgctc aaattgattg aaaaaggaat gacacgagaa gaagcttatg acttagttca
     1561 acctaagaca gcttattcct gggacaatca agtggatttc aaaccacttt tagaagaaga
     1621 caccaaagtt acctcttgtc ttacacagga agaaattgat gaactattta atccgattta
     1681 ttacacaaaa cgtgttgatg atatttttga aagactagga ttagaaaaat aaaaaaattc
     1741 cattactggt taacgttttt ttaaccagta atggaatttt tattgattta aaaggaatta
     1801 atattaaaaa aacatactta aaatgagttt attatgataa tatataataa gaagaaaaat
     1861 ttaggagtta gtttttaaag gaaaatgtta aaacattttg gtagtaaagt aagaaatttg
     1921 agagtaacta gaaatattac tcgcgaagat ttttgtggtg atgaaactga gttatctgta
     1981 agacaattag ctaggattga aagtggacag tcaatcccaa acttgactaa agctcactac
     2041 atagctaaac aattgaatgt aaaattagat attttaacag gaggagagag cttagaatta
     2101 cctaaacggt ataaagagtt gaagtatttg attttaagaa ttccaactta tgcagatgct
     2161 gaacgattaa aattaagaga atgtcagttt gatcatattt tcgaagaatt ctatgataat
     2221 cttccagaag acgagtgctt agcaattgac agtttacaag ctaaatttga agtctatcaa
     2281 acaggagaca ttaattttgg tgt
//
LOCUS       DATRUK010000115         2245 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.116, whole genome shotgun sequence.
ACCESSION   DATRUK010000115 DATRUK010000000
VERSION     DATRUK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.116"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            19..1653
                     /locus_tag="VBE34_001926"
     CDS             19..1653
                     /locus_tag="VBE34_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO7264591.1"
                     /translation="MSNWNLDNFDNLHSSLAESAYNSRPNSFPELFETDSVTEVKFSQ
                     PSEDNKGQITQGGTNLPNDGIVYLQPDKSLKSIDENVKVLIPDVNGGYHTEHYVTHSY
                     QKGVLTDDKAGFNAYYLSDTEKIDSTTKHTYLAIRGSDGIGLDTLNDWVSNNAMFAVS
                     NKYIPQAKLANKAMKEKIAELKGKAPGAVMDITGHSLGTIVSSQAVVNLSYAELENVG
                     QVVLFDGPDVSRSLEKMEGISAKKIQEAGKHVTYYVNPFDIVSMLNREKPWKDQFGKV
                     NVIVPSKYTGTFDEQSSHDFGAFQIDSSGNPLVASESYHPELLTSGQDLARLEKKFIK
                     NLLGEHYDSDKLGPVLKGALTGDLTRLIATLKACGISVTVNEAKKLYDNFQKDYKAII
                     SKAKKDGLEWDRKNIASFHSRIKSASGSQRILLRTQMLYMATQLAEADVSEKVSATKK
                     HISEAKDTIVEISTSTISSAEIMAMHLGQSEVDALVSDIKMSTVWNDSVETSDYEALD
                     HYKTKMTTFTTNLVTVAQNLTAQDEQLAGDIVTNLS"
     gene            1672..1944
                     /locus_tag="VBE34_001927"
     CDS             1672..1944
                     /locus_tag="VBE34_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264592.1"
                     /translation="MSKELKLPLNKMLKEQERIAIKAIVATAKSTITDTGTNLKATIE
                     TDAQDLKNYASTYNANITDGLEGQSAQAASDFLTQLPKPELDNPVN"
     gene            1954..>2245
                     /locus_tag="VBE34_001928"
     CDS             1954..>2245
                     /locus_tag="VBE34_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264593.1"
                     /translation="MVDTKKLQELDQEYDQNLRNIYRNREQLEDDFHLFMARTDSLKE
                     SVYQATLGQGWELPQEAHAHLYNMDDNKDTFISEFNEYMEKLEEKEIELRRVY"
BASE COUNT          822 a          401 c          432 g          590 t
ORIGIN      
        1 ggtagaggag aagaataatt gagtaattgg aacttagata attttgataa cttacatagc
       61 tctttagcag aaagtgcata taatagtcga cctaattcat ttcctgaatt atttgagact
      121 gactcagtaa cagaagtaaa attttctcaa ccctcagaag ataacaaagg tcaaatcaca
      181 cagggtggca ctaacctccc caatgatggt attgtctacc tgcaaccaga taagtcctta
      241 aaatctattg atgaaaatgt aaaggtcctc attccagatg tcaatggagg ctatcacact
      301 gagcattatg ttactcattc ttatcaaaaa ggtgtgctta ctgacgataa ggcaggtttt
      361 aatgcttact acctgtcaga tactgaaaaa atagatagca ctacaaagca cacttatcta
      421 gccattcgtg gtagtgatgg gattggtcta gatacactaa acgattgggt tagtaacaat
      481 gcaatgtttg ctgtctctaa caagtatatt ccacaagcga aattggctaa taaggcaatg
      541 aaagaaaaaa ttgccgagct aaaaggaaaa gcacctggag ctgttatgga tattactggt
      601 cactcactcg gtacaattgt atcatctcaa gcagtagtta atcttagcta tgcagaattg
      661 gagaatgttg gtcaagtagt ccttttcgat ggccctgatg tatcaagaag cttggaaaaa
      721 atggaaggca tttctgctaa aaaaatccaa gaagctggga agcatgttac ctactatgtc
      781 aatccctttg atatagttag tatgcttaat cgtgaaaagc catggaaaga ccaattcggg
      841 aaagtaaatg tcattgttcc tagtaaatat acgggtactt ttgatgagca atcttctcac
      901 gattttggtg ccttccaaat tgatagtagc ggaaatcctt tagttgcttc tgaaagctat
      961 catccagaat tattaacatc tggtcaagac ttagctagac tcgaaaaaaa attcataaag
     1021 aatcttttag gagagcatta tgattcggat aagctaggac cagtactcaa aggcgctctg
     1081 actggggacc tgactcgact aattgcaaca ttaaaagctt gtggtatcag tgtcactgta
     1141 aacgaagcta aaaaactgta cgataacttc caaaaagact acaaagccat aatttccaaa
     1201 gctaaaaaag atggcctcga gtgggatcgc aaaaacatcg caagcttcca tagtaggatc
     1261 aaatcagcat caggcagtca acggatttta cttagaactc aaatgctcta tatggcaacg
     1321 caattagcag aagcagatgt ttcagaaaaa gtctctgcaa caaaaaaaca tattagtgaa
     1381 gctaaggata cgattgtaga aatatcgaca tccactattt catcagctga aataatggct
     1441 atgcaccttg gccaatcaga agttgatgct ctggtttcag atataaaaat gtcaacagtt
     1501 tggaatgata gcgtagagac ttctgattat gaagcacttg atcactataa aacaaaaatg
     1561 accacattta cgaccaattt agtaacagtt gcacaaaatt taacagctca agatgagcaa
     1621 ctagctggtg atattgtcac aaacttatcc taaaatagag agattaaatg aatgtccaaa
     1681 gaattgaaat tgcccttgaa taaaatgttg aaagaacaag aaagaatagc tattaaggca
     1741 atagttgcta cagcaaaaag cactattacg gatactggaa caaatttaaa agcaaccata
     1801 gagacagatg cccaagacct aaaaaactat gcatccacct ataacgccaa tatcacggat
     1861 ggtcttgaag gccagtcagc tcaagcagca tctgatttct tgacacaact tccaaaacca
     1921 gagttagata atccagtcaa ctaggaggct agtatggtag atacaaaaaa actgcaagaa
     1981 ctcgatcaag agtatgatca aaatctcaga aacatttatc gtaacagaga gcaactagag
     2041 gatgactttc acttgtttat ggcaagaaca gatagcctaa aagagtctgt ctatcaagca
     2101 actttaggtc aaggttggga attaccccaa gaagctcatg cacacctata taatatggat
     2161 gataataagg atacattcat atcagaattc aatgaatata tggaaaagct tgaagaaaag
     2221 gaaatagagc ttcgaagagt ttata
//
LOCUS       DATRUK010000116         2147 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.117, whole genome shotgun sequence.
ACCESSION   DATRUK010000116 DATRUK010000000
VERSION     DATRUK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.117"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            193..465
                     /locus_tag="VBE34_001929"
     CDS             193..465
                     /locus_tag="VBE34_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEO7264594.1"
                     /translation="MRLDKYLKVSRIIKRRPVAKEVADKGRVKVNGVLAKSSTDLKLN
                     DQVEIRFGNKLLTVKVLEMKDSTKKEDAIKMYEIINETRIETDEQA"
     gene            452..823
                     /locus_tag="VBE34_001930"
     CDS             452..823
                     /locus_tag="VBE34_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEO7264595.1"
                     /translation="MSKPNVVQLNNQYINDENLKKRYEAEELRRKNRLMGWVLIFVML
                     LFILPTYNLVKSYRTLQERRQEVVKLTKDYQTLTNRTENQKLLAKQLKNPDYVQKYAR
                     AKYYFSKTGEMIYPLPDLLPK"
     gene            826..960
                     /locus_tag="VBE34_001931"
     CDS             826..960
                     /locus_tag="VBE34_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEO7264596.1"
                     /translation="MNNLIETIEHFLAYSDDKLEELADKNYQLRQTIVEKEKIESREK
                     "
     gene            960..>2147
                     /locus_tag="VBE34_001932"
     CDS             960..>2147
                     /locus_tag="VBE34_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO7264597.1"
                     /translation="MKKVLTFLLCSLYFVSIPAISTEEPLTLSQNRRYALTQTVVDKE
                     MYFDAIPERPTTKIEISSFQDEALTITGETLVPNTLLSIISLTINSNGIPVFTLSNGQ
                     FIKASREAIFNDLVSKQQSVSLDYWLKPSFVTYEAPYTNGVSEVKNNLKPYSRVHLVE
                     QAETEHGIYYKTDSGFWISVEDLSVADNRMAKVQEVLLEKYNKDKYGIYIKQLNTQTV
                     AGINIDRSMYSASIAKLATLYASQEQVKLGKLSLDSKFEYKDNVNQFPNSYDPSGSGK
                     LEKKADHKLYTVKELLEATAKESDNVATNMLGYYVNNQYDSMFQTQVDTISGMHWDMK
                     KRQISPQAAGKMMEAIYYQNGDIVNYLSKTDFDNTRIPKNIPVKVAHKIGDAYDYKHD
                     AAIV"
BASE COUNT          822 a          299 c          380 g          646 t
ORIGIN      
        1 caacttttga aagacataaa tatttttaga ggttattaaa atagaagttt tttacttgat
       61 atttgagaga attaagagag ctaatagctc tctttttgtt acattatatt gacaaatatt
      121 atcagttcag atatttttca aaaaaaatgc taaaatagta gagttaattt agatagaagg
      181 atgtgatcat cagtgagatt agacaagtat ctaaaagtgt cgcgtatcat taagcgtcgt
      241 cccgttgcta aagaagttgc agacaaagga cgtgtcaagg tgaatggcgt attagcaaag
      301 tcatcaactg acctaaagct taatgaccaa gttgaaattc gttttggtaa taaattgttg
      361 accgttaaag tattagaaat gaaagatagc actaagaaag aagatgctat caaaatgtat
      421 gaaattatta atgaaacaag gatagaaaca gatgagcaag cctaatgttg ttcagttaaa
      481 taatcaatat attaacgatg agaatctaaa aaaacgttac gaagctgagg agttacgccg
      541 aaaaaatcgt ttaatgggtt gggttcttat ttttgtcatg cttttattta ttttacccac
      601 ttataattta gttaagagtt acagaacttt acaagaacgt cgtcaagaag ttgtaaaatt
      661 aacgaaagac tatcagacat taactaatag aactgagaac cagaagttac tagcaaaaca
      721 actaaaaaat ccagattacg ttcaaaaata tgctcgagcg aagtattatt tctctaagac
      781 cggcgaaatg atttacccat taccagacct tttaccaaaa taattatgaa taatttaatt
      841 gaaacaatag aacattttct agcctactcg gatgacaaac tagaagagtt agctgataag
      901 aactatcaac ttaggcaaac aattgtagaa aaagaaaaga ttgagagcag agaaaagtaa
      961 tgaagaaagt tttaaccttc ttattatgta gcttatattt tgtatcaatc ccagctataa
     1021 gtaccgagga gccattaacg ctttctcaaa atagacgata cgccttaact cagacagttg
     1081 tggataagga aatgtatttt gatgctattc cagagagacc aactacaaaa attgaaataa
     1141 gcagttttca agatgaagct ttaaccataa caggagagac tttagttcca aatactttat
     1201 tgtcgattat ttcccttaca attaattcaa atggaattcc agtatttact ttgtccaatg
     1261 gacagttcat aaaggctagt cgtgaagcta tttttaatga tttagtttct aagcagcaat
     1321 ctgtctcatt ggattattgg ttaaaacctt cttttgtgac ttatgaagca ccttacacaa
     1381 atggtgttag tgaagttaaa aataatttaa aaccttattc cagagtgcat ttagtagaac
     1441 aagcagaaac agaacatggt atttattata aaacggacag tggtttttgg atatcagtag
     1501 aagatctatc ggttgctgat aatcgtatgg ctaaggttca agaagtatta ttggaaaaat
     1561 ataataaaga taaatatggt atatacatca aacaattaaa tacacagaca gtagctggta
     1621 ttaatattga taggtcaatg tactctgcaa gtattgcaaa attagcaaca ctttatgcta
     1681 gccaagagca agtaaaatta ggaaaactgt cattggacag caaatttgaa tacaaagata
     1741 atgttaatca atttcctaat agctatgatc caagtggtag tgggaaatta gagaaaaaag
     1801 ctgatcataa attatataca gttaaggagt tacttgaagc aactgccaag gaatcagata
     1861 atgttgcaac aaatatgcta ggttattatg tcaataatca atacgattca atgtttcaaa
     1921 cacaagtaga cacaatttct ggtatgcact gggatatgaa gaaacgccag atttctccac
     1981 aagctgcggg taaaatgatg gaagcaattt attatcaaaa tggagatatt gttaattacc
     2041 tatcaaagac tgattttgat aatacaagaa tccctaaaaa tataccagtt aaagtagcac
     2101 ataaaattgg agatgcttat gactacaaac atgatgcggc tattgtt
//
LOCUS       DATRUK010000117         2094 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.118, whole genome shotgun sequence.
ACCESSION   DATRUK010000117 DATRUK010000000
VERSION     DATRUK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.118"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..250)
                     /locus_tag="VBE34_001933"
     CDS             complement(<1..250)
                     /locus_tag="VBE34_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEO7264598.1"
                     /translation="MAYGRISLEQVLNSEDFYQLPKMMIKAKYYRKLRAEAKLIFALF
                     RDRITASLTNVKQGDMRFVDENGDIFIYYPIEELVQDLG"
     gene            complement(250..444)
                     /locus_tag="VBE34_001934"
     CDS             complement(250..444)
                     /locus_tag="VBE34_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264599.1"
                     /translation="MNHSDKKRLRAKQRQSRNLVIMSIMQQTGWARNKVAISLKELED
                     YDLIKFPSRGGMMVKVGEVR"
     gene            complement(626..1981)
                     /locus_tag="VBE34_001935"
     CDS             complement(626..1981)
                     /locus_tag="VBE34_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HEO7264600.1"
                     /translation="MKKESISIKDILWFIIPSLFGVLLLMTPFKYNGMTTVAVSVISK
                     TINQWINAVFPIHYIILLIIFISCVLALCYRLFRPSFIEKNDLLKEISDITIFWLIIR
                     LIGLALGLMTVLHIGPEMVCGKETGGLILFDLIGGLFTIFLAAGFILPFLTEFGLLEF
                     VGVFLTPIMRPFFQLPGRSAVNCVASFVGDGTIGIALTDKQYVEGYYTSREAATISTT
                     FSAVSITFCLVVLQNVGLTDYFGHFYLTVIISSVVCAFIIPYIPPLSRQSNSYYTKPK
                     KETSEVIPDGYNKIEWGLKLAIDKSKANGNMKTFVNNASKTVLSLWFGVMPTIMTVGT
                     IALIISVSTPIFKILGTPFLPFLELLGIPEADIASQTMIVGFSDMVVPSIMAAEIHSE
                     MTRFIVATVSIVQLIYMSETGAVILGSKIPINILELFIIFIERTIISLPIIVLMAHLF
                     F"
BASE COUNT          752 a          386 c          310 g          646 t
ORIGIN      
        1 aacctaaatc ttgaactagt tcttcaatcg gataataaat gaaaatatcg ccattttcat
       61 caacgaaacg catatcacct tgtttgacgt ttgtaagcga tgcagtaatt ctatctctaa
      121 aaagtgcgaa tattaattta gcttcagctc gtaactttcg ataatatttt gcttttatca
      181 tcattttagg aagttgataa aagtcttcac tatttagtac ttgttctaag gaaatccgtc
      241 catatgccat taccttacct cgccaacttt taccatcatg ccacctcgac ttggaaattt
      301 aatcaagtca tagtcttcaa gttcttttaa agagatggct actttattac gagcccaacc
      361 tgtctgttgc attatagaca taataactaa atttcttgat tgtctttgtt tagcacgtaa
      421 tcttttcttg tctgaatgat tcatacgtta tcctttcgta acatttttag catagaaaaa
      481 gagaacaatt ttcattgctc tcttgattta tagatctatt tttactaatt tctcaaaact
      541 ctagacactt actttcaagt tcatttgtct tttttcagta aatagttcta tttgttagca
      601 agaaatgctt ctctattttc tccaactaaa agaaaagatg tgccatcaaa acaataatag
      661 gtaatgaaat aattgttctc tctataaaaa tgataaataa ttctagaatg ttgataggga
      721 tcttggaacc taatatcacc gcaccagtct cagacatata aattaattgc acaatagaaa
      781 ctgttgcgac aataaacctt gtcatctcac tgtgaatttc agccgccatt atcgaaggaa
      841 caaccatatc tgaaaaacca acaatcatgg tttgactcgc aatatctgct tctggaatcc
      901 ctaaaagttc aaggaacgga aggaatggtg tacctaaaat tttaaaaata ggtgttgata
      961 ctgaaataat caaagctatt gttccgacag tcataattgt tggcataacg ccaaaccata
     1021 aactaagtac tgtcttagag gcattattga caaaagtctt catgtttcca tttgcttttg
     1081 atttatcgat ggcaagctta agtccccact ctatcttgtt ataaccatcc ggaattacct
     1141 cagatgtttc tttctttggt ttagtgtaat aactatttga ttgacgagat aagggaggga
     1201 tataagggat aatgaatgca cagacaacac tgctaatgat gactgttaga taaaaatggc
     1261 caaaataatc agttaagcca acattctgca aaacaacaag acaaaaagtg attgatactg
     1321 ctgaaaaagt agtggaaatc gttgcagctt ctctactggt ataataaccc tcaacatact
     1381 gcttatcggt caaagcaatt ccaatagtac catcacctac aaatgaggct acacaattaa
     1441 ctgctgaccg accaggtagc tgaaagaatg gacgcataat gggagttagg aagacaccaa
     1501 caaactccag tagaccaaat tctgtcaaaa atggaagaat aaaacccgca gcaaggaaaa
     1561 tggtaaacaa accaccaatc aaatcaaaaa ggataaggcc gcctgtctct ttcccacata
     1621 ccatttcagg accaatatgt aaaaccgtca tcagaccaag tgccaaaccg atcaaacgaa
     1681 taatcagcca gaaaatggta atgtcagaaa tttcttttag caagtcattt ttttcaataa
     1741 agctaggtct aaacagacga taacaaagtg ctaacacaca tgaaataaaa atgattagta
     1801 aaataatata atgtatagga aaaacagcgt taatccattg attaattgtc ttagagatta
     1861 cagagacagc tactgttgtc ataccattat atttaaatgg agtcattaat aataatactc
     1921 caaataacga cggaataata aaccacaaga tatctttaat agatatactt tcttttttca
     1981 tacaatcctc ctggataata tggaattgtt tctaaatata ctcataactt ccattgtaaa
     2041 attttataat aaaaaaaaca accttgaatt aaagaaaatt ttacttagta atac
//
LOCUS       DATRUK010000118         2088 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.119, whole genome shotgun sequence.
ACCESSION   DATRUK010000118 DATRUK010000000
VERSION     DATRUK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.119"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            464..1285
                     /locus_tag="VBE34_001936"
     CDS             464..1285
                     /locus_tag="VBE34_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7264601.1"
                     /translation="MIKLIATDMDGTFLRSDKTYDKARFSSLLTLMEKYDIKFVAASG
                     NLYDQLLLNFLEYPNRIAYVAENGGRVIDQDGTLLKETYLSNDTVAAVLSYLYQNYPE
                     TLISLSGEKRSYLERRTPINRRTELEYYMPNFIYKDHLLPLDDDRYFQMTLWVNENLV
                     SEMLLDISEHFKNHHIRLTSSGFGCIDVLPADVNKADGIAILLEKWGLKQDQVMVFGD
                     GGNDVEMLRAANISYAMSNAPEEIKAIAKYQTVSNDQDGVLETIENFLHKMSDNE"
     gene            1386..2000
                     /locus_tag="VBE34_001937"
     CDS             1386..2000
                     /locus_tag="VBE34_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEO7264602.1"
                     /translation="MLEVIDKALRDYQMKREQFFEINNQTVQEGAIVFTGDSIVEFFP
                     LKKHLGRDYPLVNRGVAGSDSYWLLENLRTQVWELLPSKVFILIGTNDIGLGHSQSEI
                     IANITDIIAEIRAESYMTEINILSVLPVSEEDDYIERVKVRNNQAIKALNKTLSVISG
                     INYIELYDLLVDEKGQLASSFTKDGLHLTDQAYAKISETIKLHL"
BASE COUNT          734 a          294 c          375 g          685 t
ORIGIN      
        1 tagagtgttt ctaataactt caaaatcctt acagggtcgt ttaatctcct tatctcatgt
       61 aaggattttt tgttgtatta atatgaccaa atttattaat atactcaatt atttattttt
      121 tcaaaacaga ataactatgt gaaaaaaaaa taatgttagg tctctcaaaa acacgtatag
      181 caatttcgaa tctcaaatat tttattatga ctattgtaat agttacgaat ctattattca
      241 actcaacaat aagaaaactc ttcagtaaat cattatgtta aacaagctct gatagtatgg
      301 agataagtat aggtaaaagt cgatttaatc tctgaaacaa aatttaactt aattaataga
      361 accgttgacg tatcaggtat caaatctttt tggtttatat gtgatataat gaaagaacat
      421 attgagatat ttggcaccct cttgaaaggc aaaggttact tgtatgatta aactaatagc
      481 aacagatatg gatggaactt ttctacgctc agataaaact tatgataaag cacggttttc
      541 ttctttatta acgttgatgg agaagtatga tattaaattt gtagctgcta gtggtaattt
      601 atacgatcaa ttgttgttaa actttttgga ataccctaat cgtatcgcct acgtcgctga
      661 aaatggtggt cgtgtcattg accaagatgg cacattacta aaagaaactt atttatctaa
      721 tgatacagtt gcagctgttt tatcctattt atatcagaac tatcctgaga cactcatcag
      781 cttatctggt gaaaaacgtt cataccttga gcgaagaact cctattaata ggcggactga
      841 attagaatac tatatgccta attttatcta taaagaccat ttgctgccct tagatgatga
      901 ccgatatttt caaatgacac tttgggtaaa tgaaaatctt gtttctgaga tgttgttaga
      961 tattagtgaa cactttaaaa atcatcatat cagattgacc tctagtggtt ttggttgtat
     1021 cgatgtctta ccagctgatg tcaataaagc ggatggtatc gctattctcc ttgaaaagtg
     1081 gggattgaaa caggatcaag tgatggtttt tggtgatggg ggcaatgatg tggaaatgtt
     1141 gagagcagca aatatttctt acgctatgtc aaatgcgcca gaagaaatta aagctattgc
     1201 taaatatcaa acagttagta acgatcaaga tggtgtgtta gaaacgatag agaactttct
     1261 tcacaagatg agtgataatg aatagaacca cgtagctttt cacgaagcat actattcaat
     1321 tctcaggtga aattttgtta taatacaaga aagcctagag aaatatttaa gaatgaagga
     1381 gaacgatgtt agaagttatt gacaaagctt taagagatta tcaaatgaaa cgagaacagt
     1441 tttttgagat taacaatcag acagtacaag aaggtgccat tgttttcaca ggagattcta
     1501 tcgttgagtt ttttccttta aaaaaacatt taggacgtga ctatccttta gttaatagag
     1561 gagtagcagg atcagattct tattggcttt tagaaaactt gagaacccaa gtatgggaac
     1621 tattaccatc caaagtattt attttaattg gaacaaatga tattggttta gggcattcac
     1681 agtcagaaat catagctaat atcacggata taattgcaga aattcgtgcg gaatcttata
     1741 tgacagagat taatatactg agtgttttac ctgttagtga agaagatgac tatatagagc
     1801 gtgtcaaggt aagaaataat caagcaataa aagctttaaa caaaacctta tcagtaattt
     1861 ctggcattaa ttatattgaa ttgtatgatt tattagttga cgaaaaaggt cagcttgcta
     1921 gcagttttac aaaagatggt ttgcacctaa cagatcaagc ctatgctaaa atttctgaaa
     1981 cgataaaact ccacctttaa aagtgtttag atactatttt taagtattta aatacacaga
     2041 attagtgaaa agaccttcta agtagaaagt tcatgtcacg gatagtac
//
LOCUS       DATRUK010000119         2027 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.120, whole genome shotgun sequence.
ACCESSION   DATRUK010000119 DATRUK010000000
VERSION     DATRUK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.120"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            315..1655
                     /locus_tag="VBE34_001938"
     CDS             315..1655
                     /locus_tag="VBE34_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001073016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEO7264603.1"
                     /translation="MNQNHSVKRSLSEVNQSVEVPHNSSFWNTLRAFLGPGALVAVGY
                     MDPGNWITSVIGGATYRYLLLFVVLVSSLMAMQLQQMAGKLGIVTRQDLAQATASRLP
                     KPLRYLLFIIIELALIATDLAEVIGSAIALHLLFGWPLLLSIMITILDVFLLLLLMKL
                     GVQKIEAFVSVLILTILIIFTYLVVLSQPDLDAMFKGFLPHHELFNISHEGKNSPLTL
                     ALGIIGATVMPHNLYLHSSLSQTRRVDYHNKSSIKKAVRFMTLDSNIQLSLAFVVNSL
                     LLVLGASLFYGHANDISAFSQMYLALSDKTITGAVASSFLSTLFAVALLASGQNSTIT
                     GTLTGQIVMEGFLHFKLPQWLIRLCTRLLTLLPIFAIALLVGGEENTLDQLIVYSQVF
                     LSLALPFSIFPLIYFTSQKSIMGEHANTKWNTYLAYLIAIILTLLNLKLIMDLL"
     gene            complement(1757..>2027)
                     /locus_tag="VBE34_001939"
     CDS             complement(1757..>2027)
                     /locus_tag="VBE34_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264604.1"
                     /translation="IIALIFITVLSLYLARVLQNAYLILSVFWAWLMVFKAHLEGNFQ
                     HLYFSFQILSLIAAIILGVSFFIIGYYNVKERYKQKRTSPYSRVL"
BASE COUNT          635 a          372 c          308 g          712 t
ORIGIN      
        1 agacagtata tctataccat aaagcaatct ttcgaactaa tcaaacaaga atcgatagta
       61 ttattggatt atatttctta aaaaattcac tacagatata gtttactcca cttaaataac
      121 ttaggtaaat ctacggaact aaaaataatc gatagaaatc tgttattttg ttatttcaaa
      181 tgtaactgtt aagaaaatag ttctctaata ctaaacagca cctcaatcga ttattaagta
      241 gcattaattt ttaattgttt ttctattttc tatttgttat aatggcttga gattactaga
      301 aagtatagta ttacatgaat caaaatcatt ccgtcaaaag atccttatcc gaagttaatc
      361 agagcgttga ggttcctcac aatagctctt tttggaacac tttaagagct tttcttggac
      421 ctggagcttt ggttgcagtt ggttatatgg atccaggtaa ctggattact agcgttattg
      481 gtggagctac ctatcgttac ttactattgt ttgttgtttt agtatcttct ttgatggcga
      541 tgcaattaca acaaatggct ggcaagcttg gtattgttac acgacaggac ttagcacaag
      601 caacagcttc tcgactccca aaaccgcttc gttacctgct atttattatc attgagttag
      661 cccttatcgc tactgattta gcagaagtca ttggctctgc cattgctctt cacttattat
      721 ttggctggcc cctgttactg tctatcatga ttactatttt ggatgtattc ttacttttac
      781 tcttaatgaa gttaggcgtc caaaaaattg aagcctttgt ttctgttctc atattgacaa
      841 ttttgataat ctttacatat ttagttgttt tatcacaacc tgatttagat gctatgttca
      901 aaggattttt accacaccat gagcttttta acatatcaca tgaagggaaa aatagtcctt
      961 taaccctcgc tcttggcatt atcggcgcaa cagtcatgcc acataatctt tatttacatt
     1021 cttctttatc tcagactaga cgcgtggatt atcataataa atcttcaata aaaaaagctg
     1081 tacgtttcat gaccttagat tctaacattc agctatcact tgcatttgtc gtgaattcgc
     1141 tactattagt tttaggagct agtctatttt atgggcatgc taatgatatc agtgcctttt
     1201 ctcaaatgta tcttgcctta tctgataaaa ctattactgg tgcagtagcc agttctttcc
     1261 tttctaccct ttttgcagtt gccctactag caagtggtca aaattcaaca attacaggta
     1321 ctttaacagg acaaatcgtt atggaaggtt tccttcactt caaattacct caatggttaa
     1381 taaggctttg tactagatta ttaactcttc ttccaatttt tgctatcgcc ctactagttg
     1441 gaggcgaaga aaatacgtta gatcaactaa tcgtctattc acaagttttt ctttccttag
     1501 ctttaccatt ttcaatcttt ccattgattt atttcacatc gcaaaaatca attatgggag
     1561 agcatgctaa tactaaatgg aatacctatt tagcatacct tatcgctatc attttaacat
     1621 tactcaattt gaaattaatc atggatttac tttaatacaa tacaaaaaag ccccactaat
     1681 gaactgaacc tctatcatga gacatacatc taatgataga ggaaagtaca taatgatgga
     1741 gctttttgta tttcttttaa agaacccgtg agtaaggact agtacgtttt tgcttgtacc
     1801 tctccttgac attataataa ccgatgatga aaaacgatac gccaaggata attgctgcaa
     1861 tcaaagataa aatttggaaa ctaaaatata ggtgttgaaa atttccttct aaatgtgctt
     1921 taaaaaccat caaccaagcc caaaaaacgg ataagattaa gtaagcattt tgtaataccc
     1981 tagccaggta aagacttaaa acagttataa aaatcaaggc aatgata
//
LOCUS       DATRUK010000120         1971 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.121, whole genome shotgun sequence.
ACCESSION   DATRUK010000120 DATRUK010000000
VERSION     DATRUK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.121"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            944..1162
                     /locus_tag="VBE34_001940"
                     /pseudo
     CDS             944..1162
                     /locus_tag="VBE34_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001889697.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1818..>1971
                     /locus_tag="VBE34_001941"
     CDS             1818..>1971
                     /locus_tag="VBE34_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7264605.1"
                     /translation="MTLFLIMMERAGLIILLAYAFVHIPFIKQTLKQPELKKHQYILL
                     ILFSLFA"
BASE COUNT          728 a          243 c          348 g          652 t
ORIGIN      
        1 ttgtcgattc cctttatgca gcatattata ttcataaata tcccgaatat gagtgataaa
       61 ttaattaatt taaggtagga gcaataaata tctagagaaa atgaagtggg tagaatagtc
      121 tctacgcttg ctgagagtgt ttttgtagta atcataacaa ctccaatttg taccagtagt
      181 agcagttgtt ataggtactt tggccattgt ggagtaaaag cactctatga aaatgttaaa
      241 gatgtaagaa attttgtgga gccgtttcat gagcaaatat tattgatcaa gattatattt
      301 tgattatctg gcaggatttt tccagagagg tcaatttaga acaagtatat tatttcaatg
      361 agaagatatt gatgaagtaa ttttgggagg taccatgacg aggagtaaga aagagtagct
      421 cgtatcattg aaaaatggaa aaatatttct aagggtaaga ctattgtaga atagaaataa
      481 tacacaaaaa gggaaattaa atctcatgaa attaagggaa atagtaaaaa tattttttga
      541 ttttttatcg ttatgtttta taaatactct ggtgatattg atttttttaa tagataatag
      601 aattcatatt cagtttctag tgccaaaacc tatgccatta attgatggaa tgaccctctg
      661 taggatttaa agtactttgt ttaactaatg atgcgtgcgc atcattagga ataggagtat
      721 cggcgatatc aatagttggt ataacaagcg aaattgcaca agtaaaatta aaaccaggac
      781 cagttgaaat aaaattatcc gttggtttgt aggaaggatt agttttggga ttattactaa
      841 aattgactgg aaactctgag agtttagttc ttcaaatata tgatattgat acgaagaagt
      901 gggaagaaag aatcagtcta aaaaagtaga agaaggtttt tttgtgaaat caaaaatagc
      961 taatcttgaa tatcatacaa ataataatat tacttcaaat gaaagagtat tagcttggtc
     1021 tgataaagaa cctacaaata atatgaattt aaaaatcttg agtcttacat tgatatgtat
     1081 accgtaacta tattgcattg agttggttgt ttgcgatagc aacgtcaaga cagcaataaa
     1141 attgaaaaaa tcaataagat aaaaaggatt taaaaaattg attattattg tggaagggat
     1201 aagcttaatg atgattttaa cagcatgtga cttagtaaaa aaagatcacc aatgggttca
     1261 agaacattga aaatacttag aagctggatc aaaagttatc aaagaagtca gccaaaacat
     1321 tcaaccagtt atttctgatc tgaatagcta tctcaatgga gcgacaagtg cttttgagca
     1381 atcggattcg agtgcagcta ctcatattaa aaataacacc atcgcccaac ttaaaaaaag
     1441 cagtaccaaa tcgtgtgaga aattaccagg ctaattgtga gtgagaatct attgttctca
     1501 cttttttatt atcaaaacgc ttaaattctt tacttagacc tcgcctacac acttcagata
     1561 atgggtgtta ccatattaat taataaatat gtaaaaaata atatttgata tgctataata
     1621 ataatatatt tgattaatca aaatttttta gtgaaagtat ctatcatcct taagtagttg
     1681 ttttcataat agttgttgat ggatatcccc ttaattgtct tgagaatact tgatagagag
     1741 acattttaaa tgagtaatga aatgaatcat tttataccta tgttataacg gactaattaa
     1801 tactttggag agatttaatg acactctttt taattatgat ggagcgagcg gggttaatca
     1861 tattattagc ctatgccttt gtgcatattc cctttataaa acaaactttg aagcaacccg
     1921 aattaaaaaa acaccagtat attctgctta tcttatttag cttatttgct a
//
LOCUS       DATRUK010000121         1927 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.122, whole genome shotgun sequence.
ACCESSION   DATRUK010000121 DATRUK010000000
VERSION     DATRUK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.122"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            61..1047
                     /gene="dhaQ"
                     /locus_tag="VBE34_001942"
     CDS             61..1047
                     /gene="dhaQ"
                     /locus_tag="VBE34_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HEO7264606.1"
                     /translation="MRKTEHHKMEYVFETIKAITKTNPKVEQIKDLPAIHYLGQSEQV
                     IPILSGGGSGHEPAHFGYVGEGMLSAAISGPIFVPPCASDILETIRFINRGKGVFVII
                     KNFEADLEEFSQAIEQARQEGIPIKYIVSHDDISVETSNFKIRHRGVAGTVLLHKIIG
                     QAALEGASLDELEQLGLSLTTSMATLGVASKSATILGQHQPVFDLEEGYISFGIGIHG
                     EPGYRTMPFVSMEHLANELVNKLKMKLRWQDGEAFILLINNLGGSSKMEELLFTNAVM
                     EFLALDDLQLPFIKTGHLITSLDMAGLSVTLCRVKDSRWIDYLKHKTDARAW"
     gene            1302..1577
                     /locus_tag="VBE34_001943"
     CDS             1302..1577
                     /locus_tag="VBE34_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264607.1"
                     /translation="MKKNSLYNQLINSEPIGFIDPLTDLGEFDIFQMKFKEPVRYLKN
                     KYSGKPYSPHWQNKIEKMRSHQESLREEVKEEIPITSRARNQENNLS"
     gene            1638..1927
                     /locus_tag="VBE34_001944"
                     /pseudo
     CDS             1638..1927
                     /locus_tag="VBE34_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
BASE COUNT          701 a          261 c          379 g          586 t
ORIGIN      
        1 aataggaaag tcattgaaaa aattatcaga acatcaatag aaaattatta ggacaaggat
       61 atgagaaaaa cagaacatca taaaatggaa tatgtttttg aaacaataaa agcaatcact
      121 aaaactaatc ctaaagtaga gcaaataaaa gatttgcccg ctattcatta tttagggcaa
      181 tccgagcaag taattccgat tttatcagga ggaggcagcg gacatgagcc ggctcacttt
      241 ggatatgttg gtgagggaat gctatcggca gcgattagtg gtcctatttt tgtaccacca
      301 tgtgctagtg atattctaga gactattaga tttatcaatc gaggcaaagg tgtctttgtt
      361 attatcaaaa attttgaagc tgatttggaa gaattttccc aagcgattga acaggctcgt
      421 caagaaggca ttccaatcaa atacatcgtt tctcatgatg acatctctgt tgaaacatca
      481 aattttaaaa ttcgtcatcg aggcgttgct ggtactgtgt tgctacataa aattattgga
      541 caagctgctt tagaaggagc aagcttagat gaattagaac agctaggact atctttgaca
      601 acttcaatgg ctactttagg tgtagcaagt aagtcagcca ctattttagg tcagcatcaa
      661 cctgtttttg atttagaaga aggttacatc tcatttggaa tcggtattca tggtgagcca
      721 ggataccgga caatgccttt cgtgtccatg gaacatttgg caaacgaact agttaacaaa
      781 ttgaaaatga aattacgttg gcaagatgga gaagcattca ttctattgat aaataatctt
      841 ggaggaagtt ctaaaatgga ggaattactt tttacaaatg ctgtcatgga atttctggct
      901 ttagacgatt tacaattacc ttttattaaa acaggccacc taattacaag tttagatatg
      961 gctggtttat cagttacctt gtgtcgtgta aaggatagta gatggattga ttacttaaaa
     1021 cataagacag atgctagagc ttggtgatag aagtaagtgg taagagtttt tagaatagaa
     1081 aatactttaa ggagaacaga gaaatctgtt ctctttttgt tcgcttaaag atcatagaaa
     1141 ggtgaagaga acatggaagg tattgaaata atattagata tagaaacgtt aggaattttt
     1201 caagtcatag atcatggtat ctctccagat gatagagaaa catatgaaca attaactttg
     1261 ttttaaataa cttaacttta ttatcttaga ggatgttaca tatgaaaaag aatagtttat
     1321 ataatcagtt aataaatagt gaaccgatag gctttattga tccactaacc gacttggggg
     1381 aatttgatat ttttcaaatg aagttcaaag aacccgttag atacttgaaa aataaatatt
     1441 caggaaagcc ttattcacca cattggcaga ataaaattga aaaaatgagg tctcatcaag
     1501 aaagcctaag agaagaagtt aaagaagaga ttccgattac tagtagggca agaaatcaag
     1561 aaaataattt gagttgatct taaattgata aaaagttcga cttttttcct aaaaaatagt
     1621 gtgctataag tcaattaaaa gatgtgctat atttatttaa tagatatcgt ctcattgaat
     1681 atcaactaac cggagaaaat tcgaatgcaa aaaatttaat gacaggcact caaaatttaa
     1741 atgctaattt tgaagatgaa aaatcgtcga tggtatatta tgaaaatatg gtggctcatt
     1801 atataagact aacaaaccat catgtgagat accgtgttac actcttattt aaaaatattg
     1861 agatggttgc aagaggggtt ggtatggaaa cccaaagtgt ggaagataat cagatatctt
     1921 ttgatat
//
LOCUS       DATRUK010000122         1823 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.123, whole genome shotgun sequence.
ACCESSION   DATRUK010000122 DATRUK010000000
VERSION     DATRUK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.123"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(44..1141)
                     /locus_tag="VBE34_001945"
     CDS             complement(44..1141)
                     /locus_tag="VBE34_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7264608.1"
                     /translation="MRIIAITEKVIKELFRDKRTLAMMFLAPILIMFLMNVMFSANSN
                     TKVKIGTINVNTKVVSNLDNIKHIQVRSFKFNSSAKKALKSNKIDALISEDNKSYTVF
                     YANTDSSKTTLTRQAFKTAVNTMNSKELISQVKILANKNPKLAQSLQTRSKYIKEKYN
                     YGNKNTGFFAKMIPILMGFMVFFFVFLISGMALLKERTSGTLDRLLATPVKRSDIVFG
                     YMLSYGILAIIQTIVIVLSTIWLLDIQVVGSIFSVIIVNFILALVALSLGILMSTLAK
                     SEFQMMQFIPLIIMPQLFFSGIIPLENMASWAQTVGKILPLSYSGDALTKIIMYGQGL
                     PNVSSNLLVLLLFLIILTIANIFGLKRYRKV"
     gene            complement(1144..>1823)
                     /locus_tag="VBE34_001946"
     CDS             complement(1144..>1823)
                     /locus_tag="VBE34_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7264609.1"
                     /translation="YASETVLNNINLEVFKGEIIGLIGPSGAGKSTLIKTMLGMEKAD
                     KGTALVLDTQMPDRNILNQIGYMAQSDALHESLTGLENLLFFGKMKGIQKTELKQQIT
                     HISKVVDLENQLDKFVSGYSGGMKRRLSLAIALLGNPTVLILDEPTVGIDPSLRRKIW
                     QELINIKDEGRSIFITTHVMDEAELTSKVALLLRGNIIAFDTPLHLKKQFNVSTIEEV
                     FLKAEGE"
BASE COUNT          607 a          292 c          287 g          637 t
ORIGIN      
        1 aaaataatat tatttttcat acttagttcc ttttcaaatg gtattatact ttacggtatc
       61 gttttagtcc aaagatattt gcgatggtta aaataattaa aaatagaaga agtaccagaa
      121 gatttgaaga aacgttaggt aatccttgac cgtacataat tatttttgta agtgcatcac
      181 cagagtagga tagcggtaga attttaccga ctgtctgagc ccaacttgcc atattttcta
      241 gagggattat tcctgaaaag aataattgag gcattataat taagggaata aattgcatca
      301 tttgaaattc tgattttgct agagtagaca ttaagattcc taatgataag gcaaccagag
      361 ccaaaataaa attgacaatg atgacactaa aaatactacc tacaacttga atatctagta
      421 gccaaatagt tgataaaacg ataactatcg tttgaataat tgctagaatg ccgtatgata
      481 acatatatcc aaatacaata tcactacgtt taacaggagt tgcaaggagt cgatcaagtg
      541 taccagaagt acgctctttt aataaagcca ttccagaaat taaaaaaaca aagaagaaga
      601 ccataaatcc cattagtatt ggtatcattt ttgcaaaaaa gcctgtattt ttatttccgt
      661 aattatattt ttctttgata tatttggagc gagtttgtaa ggattgtgct agtttcggat
      721 tcttattagc taaaatttta acttgcgaaa tcagttcctt actgttcatt gtattaacag
      781 cggttttaaa agcttgtctt gttaaagtcg tctttgaaga atctgtattc gcatagaaga
      841 cagtataaga tttattgtcc tccgaaataa gagcatcaat tttatttgat ttgagtgctt
      901 ttttagcaga tgagttaaat ttaaatgatc tcacttgaat atgcttaata ttatctaaat
      961 ttgaaacgac cttcgtgtta acgttaatag ttccaatctt aacttttgta ttactattcg
     1021 cagaaaacat aacattcatc aaaaacataa ttaaaatagg tgctaaaaac atcatagcaa
     1081 gtgttctttt atcacgaaac agttctttta taaccttttc agttattgca ataattctca
     1141 tgattattct ccttcagctt ttaagaaaac ttcctcaata gtactcacat taaattgttt
     1201 ttttaaatgt aatggagtat caaaggcaat aatgtttcca cgtaatagta gtgcaacctt
     1261 acttgttaat tctgcttcat ccataacgtg ggttgtaata aagatagaac gtccttcatc
     1321 cttaatatta attagctctt gccagatttt tctcctcaag gatggatcaa ttccaacggt
     1381 aggttcatct aggattaaaa ctgtggggtt tccaagtagg gcgatggcta gagaaagccg
     1441 tcttttcata cctcctgagt aacctgagac aaatttatca agttggtttt ctagatctac
     1501 tactttagaa atatgagtta tctgctgttt taattcagtt ttttgaatac ctttcatttt
     1561 tccaaagaat aataaatttt ctaagccagt taaagactcg tgtaaggcat cagattgagc
     1621 catatagcca atttgattta aaatattacg atctggcatt tgagtatcaa gaacaagagc
     1681 tgttccctta tctgcttttt ccatgccaag catagtttta atcaaggtag atttccctgc
     1741 tccagagggt cctattaatc caattatttc tcctttaaac acctccaaat taatattatt
     1801 taaaacagtt tctgaggcgt atg
//
LOCUS       DATRUK010000123         1796 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.124, whole genome shotgun sequence.
ACCESSION   DATRUK010000123 DATRUK010000000
VERSION     DATRUK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.124"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..393
                     /locus_tag="VBE34_001947"
     CDS             <1..393
                     /locus_tag="VBE34_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7264610.1"
                     /translation="YNLIDYLSPIENIRLVNKSVDESILFELGLDKKQIKRNVMKLSG
                     GQQQRVAIARALVSDAPIILADEPTGNLDSVTAGEIINILKELAQDRNKCVIVVTHSK
                     EVADSADIILELSGKKLKKVNKMNLEVE"
     gene            393..1769
                     /locus_tag="VBE34_001948"
     CDS             393..1769
                     /locus_tag="VBE34_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEO7264611.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMATLSIISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTNSIDSYVKRINSVADLV
                     DHDIIETQDTLANQSPERAKNFKRTVMLTGVNDSAKETKFVSEAYKLVEGKHLENKDK
                     NKILMHKDLAKKNNLKVGDKIKIKSNLFDADNEKVANETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHSAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFVGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFIIEMVFISIPALLGSYFLAQYTADKLGNNILNKVTGDIAKQIARQ
                     SASSQLGGGAEAEGFNKTLSGLDINVLPKFIIYVVIFMSFVLLVSLILSSIYTLRKNP
                     KELLIDNN"
BASE COUNT          713 a          218 c          316 g          549 t
ORIGIN      
        1 tataatttaa tagattattt atcgccgatt gaaaatatta gactagtaaa taaatcagta
       61 gatgagagta tcttgttcga attaggttta gataaaaaac aaataaaaag aaatgttatg
      121 aaattatctg gtggtcagca acaaagggta gctattgcta gggcactggt atcagatgcc
      181 ccaataatac tagctgatga gcctaccggt aacctagaca gtgttactgc tggagaaata
      241 attaatatat taaaggaatt agctcaagat agaaataaat gtgtaatagt tgtaacacat
      301 agtaaggaag tagcagattc tgcggatatc attttagaac taagtggtaa aaagttgaaa
      361 aaagtaaata aaatgaattt ggaggttgaa taatgataaa aaatgcattt gcttatgtaa
      421 ctagaaaaag cttaaaatca ttaattatta tattagttat tctatcaatg gcaactttaa
      481 gcatcattag tttatctatt aaagatgcca cagatagagc ttctaaggaa acatttgcta
      541 atataacaaa cagtttttct atggaaataa atagacaagt aaatccagga acacctaggg
      601 gtggaggaaa tgtaaaaggt gaagatatta aaaagatatc tcaaacaaac agtatagact
      661 cctatgtaaa aagaataaac agcgtcgcag acctagttga tcatgatata attgaaactc
      721 aagatacact tgcaaatcaa tcacctgaaa gagcaaagaa ttttaagaga acagttatgt
      781 taacaggggt taatgactca gcaaaagaaa caaaatttgt atcagaagca tataagttag
      841 tagaaggaaa gcatttagaa aataaagata aaaataaaat tttaatgcat aaagatttgg
      901 caaaaaaaaa caatctcaaa gttggagata aaataaaaat aaaatctaat ttgtttgatg
      961 ctgacaatga aaaagttgca aatgaaactg tagaagtaga aattaaaggt ttatttgatg
     1021 gtcataatag cggtggggta agtgcagcac aagaactata cgaaaataca ctaatcactg
     1081 acgttcattc agctgctaaa gtttatggta atacagaaga tacagccgta taccaagatg
     1141 caacattctt tgtaaaaggt gataagaatc ttgatagtgt aataaaagat cttgggaaac
     1201 tagatataaa ttggagagaa tataatttga ttaaaagttc atcaaattat cctgcgctgc
     1261 aacaatctat atccggtatc tattctattt caaacaaatt atttgttggc tcattaatat
     1321 ttgcaggagt tgtagtttca ttattacttt tcttatggat gaatgccaga aagaaagaaa
     1381 tagcagtatt actatcatta ggtatatcta aattagaaat ttttggtcaa tttataatcg
     1441 agatggtatt tatatccatt cccgcacttc ttggatctta tttcttagct caatatacag
     1501 ctgataagct agggaataat atattgaata aagttactgg cgatatagct aaacaaatag
     1561 ctagacaatc tgcatctagt caattaggcg ggggggcaga agctgaagga ttcaacaaga
     1621 ctctttcagg tttagatata aatgtattac ctaaattcat aatttatgta gttatattta
     1681 tgagttttgt acttcttgta tcattgattc tttcttcaat ttatacatta agaaagaatc
     1741 caaaagagtt attaattgat aataattaaa attttcggtg atttatatca ccgaat
//
LOCUS       DATRUK010000124         1677 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.126, whole genome shotgun sequence.
ACCESSION   DATRUK010000124 DATRUK010000000
VERSION     DATRUK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.126"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(132..1292)
                     /locus_tag="VBE34_001949"
     CDS             complement(132..1292)
                     /locus_tag="VBE34_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264612.1"
                     /translation="MIKFTYSANFFYYNCSKRIKEKMDSINQLIDEYGYTSSRLAANR
                     NTARKPKISRNAITLDYTKEFSRALNNKITQRNPNFITPALTEDILNFCKPEEDFIRQ
                     LNIDGIDSCKYEFESVSEIYWGTDEELYKNEELFFYAIIKDMLNSIDDKNFIESILID
                     YVPYAYLTALNEIDSSLPPNAVSIFDYYPDLPNLKETQKTLSEAISRLYQQFKNQYSL
                     TDMFFDFLSKNKKEVEFKNMQTEFKNFVNMYLKPRLKHMVTDENSLGKRVYELFKVDG
                     GIYITSQIFQTTISQPNKDLLDATIDYCNTLRNIQLEQENGLQSLSVELENYTQSIAL
                     SILALDKYPTGVTTIDKLLEKFESHSGGDLSAFKRIEYLEDESENDGQIYKY"
BASE COUNT          548 a          254 c          265 g          610 t
ORIGIN      
        1 acaaatccag acttgtatca gcgatttaat aatattcatg aactaaaaaa tgcattttct
       61 cagattaaac cgaaataggt tttaataaaa aggcttcaac tatcatttag tagttgaagc
      121 cttttttgct attaatattt ataaatttga ccgtcatttt ctgattcatc ttcaaggtat
      181 tctattctct taaaagctga aaggtctccc ccactatgag attcaaattt ttcaagtaat
      241 ttatcgatgg tagtgacacc tgtaggatac ttatctaaag ctaagattga taaggcaatt
      301 gattgggtat aattttctaa ctcaaccgag agactttgaa gaccattttc ttgttccagt
      361 tgtatgtttc ttagagtatt acaataatca atagtagcat ctagaagatc tttgtttggt
      421 tgtgaaattg tggtttgaaa gatttgacta gtaatgtata ttccaccatc tactttaaat
      481 aactcataga ctcttttacc gagagaattt tcatcggtta ccatgtgttt taagcgaggc
      541 tttaaataca tatttacgaa atttttaaat tcggtctgca tatttttaaa ctcaacttct
      601 tttttatttt tagaaagaaa atcaaaaaac atatctgtaa gtgagtattg atttttaaac
      661 tgttgatata gtcttgaaat tgcttcggag agagtttttt gcgtctcttt taaattaggc
      721 aaatcaggat aataatcaaa aattgaaaca gcatttgggg gcaaacttga atcgatttca
      781 tttagtgctg ttaagtaagc ataaggaaca tagtcaatca gtatactttc aataaagttt
      841 ttatcgtcaa tggaatttag catatccttg atgattgcat agaaaaataa ttcttcattc
      901 ttatagagtt cttcatctgt tccccaataa atttctgaaa cagattcaaa ttcatattta
      961 caagagtcaa taccatctat atttagttgt ctaataaaat cctcttcagg tttacagaaa
     1021 tttaatatat cttctgtaag tgctggtgtg ataaaattag gattcctttg ggtgatttta
     1081 ttatttaaag ctcgagagaa ctcttttgta tagtcaagtg taatagcatt tctactaatt
     1141 tttggttttc tagctgtatt tctattagca gctagacgac tagaagtata accatattca
     1201 tcaattaatt ggttaattga gtccattttt tcctttattc tctttgaaca attgtaataa
     1261 aagaaattgg cactatatgt aaatttaatc ataaaaagct cctaaaaaat tttgtacttc
     1321 tattataacc taataatgtt ctttgctaag gggagaattt ggacttctta ttctctattt
     1381 tttcccctta gcaaagaatt tgcttccttg tataatgaga atagagctga cagccactcc
     1441 caatcaaatg tttttggggg cgaaaatagt gaacaaaagt ttgaaaactc cccttagcaa
     1501 agttctctct actttatata ataagggtag agctggcagt cagtcccaac caaatattgg
     1561 gggcgaaaat agtgaactaa aagtttgaaa actcccctta gcaaagttct ctctacttta
     1621 tataataagg gtagagttgg cagtcagtcc caaccaaata ttgggggcga aaatagt
//
LOCUS       DATRUK010000125         1633 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.127, whole genome shotgun sequence.
ACCESSION   DATRUK010000125 DATRUK010000000
VERSION     DATRUK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.127"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..373
                     /gene="asp1"
                     /locus_tag="VBE34_001950"
     CDS             <1..373
                     /gene="asp1"
                     /locus_tag="VBE34_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEO7264613.1"
                     /translation="FKEEQKTKIRGISFDIYTNENQIIDALDTARLVIDLRDDPDLYT
                     QIAALSSGIPQINKVHTNYVEHQKNGWIISDSTELNNAIDYYFDGLMNWNKSLVYSVK
                     KMGDYTSGKILQRWRKLLEKE"
     gene            375..>1633
                     /gene="asp2"
                     /locus_tag="VBE34_001951"
     CDS             375..>1633
                     /gene="asp2"
                     /locus_tag="VBE34_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEO7264614.1"
                     /translation="MEKLKILQIGEEPWDADLITPDNIEWLYCNGNAIQIFLEKLKSK
                     ELEKNNVEYIGKTSTNEVKLWFNAVILTSEVSELQLNLLSDCLEAHTLFYDEDFQLDF
                     CSENGIFKRKVLRPLPFNGSRKEKIIFLSRTLFSEQYGAKLKVSDIDLNPDFKGEIIY
                     EGNSSICFSGNFGEDFQPLFSFRYNLSSTDKAIEIWQEYQKLQGDNSIMIEVIQYQKG
                     SLDLILNTKYLSENDLADPYILEYDESVGFSSISIFARGDGVVRFGALHWRYSRKGLG
                     QFLIGGERYSDSKRQEFIYYFNPGDMKPPLNVYFSGFRGAEGFEGFYMMKRLGAPFLL
                     IGDPRIEGGSFYLGTSEYESELENVLQKYLDYLQFDHCQLILSGLSMGSFGALYYGST
                     FSPHAIVVGKPFTNLGDMVVNLKLRRP"
BASE COUNT          563 a          216 c          312 g          542 t
ORIGIN      
        1 ttttaaggaa gaacaaaaaa ctaaaattcg tggcattagt tttgatatat atacaaatga
       61 aaatcaaatt atagatgctc tagatactgc taggttagtt attgatttaa gagatgatcc
      121 agacctatat actcaaattg ctgctctgag ttctggaatt cctcaaatca ataaagttca
      181 cactaattat gtagaacacc agaagaatgg ctggattatt agtgactcta cagaactcaa
      241 taatgcaatt gactattatt ttgatggact aatgaattgg aataaatcgt tagtttacag
      301 cgtaaaaaaa atgggtgatt atacaagcgg taagatatta cagcgctggc gaaaattatt
      361 agaaaaagaa taatatggaa aaattaaaaa ttttgcagat tggtgaggaa ccatgggatg
      421 ctgatttaat aactccagac aatattgaat ggttatactg taatggaaat gctattcaaa
      481 ttttcttaga gaaattaaaa agtaaagagt tagaaaaaaa taatgttgaa tatattggta
      541 aaacttcaac taatgaagtt aaattatggt ttaatgcggt cattttaact agtgaggtta
      601 gcgaattgca attgaaccta ctttcagatt gcttagaagc gcatacactc ttttacgacg
      661 aggattttca actcgatttt tgctcagaaa acggtatatt taaacgtaag gttttgagac
      721 cactaccatt taatggatca aggaaagaaa aaattatttt cctaagccgt actctttttt
      781 cggaacaata tggtgcaaaa ttgaaggttt ctgacataga tctaaatcct gattttaagg
      841 gagagataat atatgaaggc aatagctcta tttgtttttc agggaatttt ggagaagatt
      901 ttcaacctct attctcattt cgctataatt tatcctctac tgataaagcg atagaaatat
      961 ggcaagaata tcaaaaattg caaggtgata atagtattat gatagaagtc attcaatacc
     1021 aaaaaggatc acttgatttg atattaaata caaaatacct ttcagaaaat gatttggctg
     1081 atccatatat tttagaatat gatgaatcag ttggtttttc atcaatttct atctttgcta
     1141 gaggtgacgg agtggtgaga tttggtgcac tacattggcg atattcgcgt aaaggtttgg
     1201 gtcaattttt aatcggtgga gaaaggtaca gtgattccaa aaggcaagag tttatctact
     1261 attttaaccc tggagatatg aaacctccat tgaatgttta tttttcaggt tttcgaggag
     1321 ccgaaggttt tgagggtttt tacatgatga aacgtttggg agctccgttt ctcttaattg
     1381 gtgatccaag gattgaaggt ggaagcttct acctaggtac ttcagaatac gaaagtgaat
     1441 tagaaaatgt tttacaaaaa tatttagatt acctacaatt tgatcattgt caacttattt
     1501 tatcaggttt atctatggga agttttggtg ccctttatta tggttctact ttttcccccc
     1561 atgctatagt tgtggggaaa ccctttacaa atttaggcga tatggtagta aatttaaaat
     1621 taagaagacc aga
//
LOCUS       DATRUK010000126         1631 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.128, whole genome shotgun sequence.
ACCESSION   DATRUK010000126 DATRUK010000000
VERSION     DATRUK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.128"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            358..1631
                     /gene="ltrA"
                     /locus_tag="VBE34_001952"
                     /pseudo
     CDS             358..1631
                     /gene="ltrA"
                     /locus_tag="VBE34_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          523 a          318 c          394 g          396 t
ORIGIN      
        1 gcgtatatag cggatgaggg ggctataaac tctaaagtcc aaataggtag tcgtaaccta
       61 tatacgtaaa tcacgagagt aaacgaggaa agatgtccga cttatcccgt gaggtcctgt
      121 caagggtaaa acctagtaac aacgaacgac aagaagtcag ccgaacccat agtagtgatg
      181 aagtttctgt aatggaaatg gaacgaaggg gtgaacaact aatcgaagta atcctacttc
      241 acttgtgtct gtaaaacaaa cggtctgata gaactagatg cggttacgta ttgactagag
      301 gaaggttcac caatgtaaga tatcatgcag acaacgaaaa aagaaaggaa tacgcacatg
      361 tcagaattgc tagataagat atcatctcgg aacaatatgc tcgaagctta caagcaagtg
      421 aaatcaaaca aaggttctgc tggtatcgat ggggtcacta tcgagcagat ggatgactat
      481 cttcaccaaa attggcgaga aaccaagaaa ctcatcaaag agaggagcta taaacctcaa
      541 ccggttctca gggttgaaat cccaaaacca aacggaggag ttcgtaacct aggtatcccg
      601 acggctatgg atagaatgat tcagcaggcc atcgttcaag ttttgagtcc actctgcgaa
      661 aaacattttt cagagtatag ctatgggttc agacccaatc gctcctgcga aacagccatt
      721 gttcagctac ttgagtattt aaacgatggc tacgagtgga ttgtggacat tgacttggaa
      781 aagttcttcg atactgttcc gcaagacaga ttgatgtccc tggttcataa tatcattcaa
      841 gatggcgata cggagtcact gattcgtaag tacttccatt cgggagttgt tattaacgga
      901 cagcgacata agactttagt cgggacacct caaggcggga atctatcacc cctcctatct
      961 aatattatgc ttaatgagtt agacaaaggg ttggaaaagc gaggtcttcg ctttgtccgt
     1021 tacgccgatg actgtgtcat cactgtcgga agcgaagcag ctgctaagcg ggtcatgcat
     1081 tcggtcagta gctatattga gaagcgatta gggttgaaag tcaacatgac taagaccaag
     1141 attgtcagac cgaacaaact caaatacctc ggatttggtt tctggaaatc tccaaaaggt
     1201 tggaagtgtc gtcctcacca agacagcgtt caggctttaa gcgaaaactg aagcaactga
     1261 cgatgaggaa atggagcatt gacctgataa ctcgcattga acgattgaac tgggtcattc
     1321 gaggatggat aaactatttc tcgcttggca atatgaagag tatcatgaca caaatagatg
     1381 agcgtctgcg aacccgtatt cgagtgatta tctggaagca atggaagaag aaagcaaagc
     1441 gcctatgggg actcttaaaa ctaggagttg ctagatggat agccgataaa gtttctggat
     1501 ggggtgacca ctatcagttg gtagctcaga agtcggtact caaacgtgct atatcaaaac
     1561 cagccctcgc aaagcgagga ctggtcagtt gcttagatta ctatcttgaa cgacatgcgt
     1621 taaaagttag t
//
LOCUS       DATRUK010000127         1602 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.129, whole genome shotgun sequence.
ACCESSION   DATRUK010000127 DATRUK010000000
VERSION     DATRUK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.129"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..1309
                     /locus_tag="VBE34_001953"
     CDS             <1..1309
                     /locus_tag="VBE34_001953"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEO7264615.1"
                     /translation="QLYKVLNIIKSDYTRRFIVVSAPGKRYEEDLKMTDALIQYYQNY
                     INGKDIVKDQTWIINRYQEIISDLSLGSTIAEEITRSIEQLASLPIENNQFLYDCFLA
                     AGEDNNAKLVATFFNQNDIPARYVHPNEAGIIVTKEPCNARIIPGSYDKIENLCLYNE
                     VLVIPGFFGVTEDNQICTFSRGGSDITGSLIAAGIKADLYENFTDVDGIFAAHPGVVK
                     NPHAIPELTYKEMRELAYAGFSVLHDEALLPAYRGRIPLVIKNTNNPQQPGTKIVLKH
                     TRSNIAVTGIASDSRFASINVSKYLMNREVGFGRKVLQILEDLNISFEHMPTGIDDLS
                     IVLREKELTPIKEQEILNYLTRKLEVDYVDIQHNLSTIVIVGENMKSQIGVTATATQA
                     LSREKINITMISQGSSEVSIMFVIDSKDEKRAIKALYETFFQK"
     gene            complement(1525..1598)
                     /locus_tag="VBE34_001954"
     tRNA            complement(1525..1598)
                     /locus_tag="VBE34_001954"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1562..1564),aa:Asn,seq:gtt)
BASE COUNT          571 a          284 c          274 g          473 t
ORIGIN      
        1 acaattatac aaagtactaa atattatcaa atcagactac actagacggt ttattgtcgt
       61 ttcagctcca gggaaacgct acgaagaaga cttaaaaatg acagatgctt tgatacaata
      121 ttatcaaaat tatattaacg gtaaagatat tgttaaagac caaacttgga ttatcaatcg
      181 ttatcaagaa attattagtg atttgtcttt aggaagcact attgcagaag aaattactcg
      241 ctctatagag cagctagctt cacttcccat agaaaataat cagtttttat atgattgttt
      301 tttagcagcc ggtgaagata acaacgcaaa gttagttgca acgtttttta atcaaaatga
      361 cattcctgca agatatgttc atccaaacga agcaggaatt attgtaacta aagaaccatg
      421 taatgcacga attattccag gaagttatga taagattgag aacttatgtc tatacaatga
      481 ggttcttgtt atccctggat tttttggagt cacagaagat aaccaaattt gtaccttttc
      541 aagaggggga tctgacatta ctggatccct aattgcagca ggcataaaag cagaccttta
      601 tgagaacttc acagatgttg atggtatatt tgcagcgcat ccaggtgtag ttaagaaccc
      661 tcacgctatc cctgagctta cttataaaga aatgcgtgaa ttagcctatg cgggtttttc
      721 ggttttacat gatgaagctt tacttcctgc ctatcgtggc agaatccctc ttgttattaa
      781 aaatacaaat aatccccaac agcctggtac aaaaatagtt ttaaagcata ctcgtagtaa
      841 catagcagta actgggatcg cttctgatag ccgttttgct agcataaacg tatctaaata
      901 cttaatgaat agagaagtag gtttcggccg aaaagtacta caaattttag aggatttaaa
      961 tattagtttt gaacatatgc caactggcat agatgatcta tccattgtct tacgtgaaaa
     1021 agaattgaca ccaatcaaag aacaagaaat cttaaattac ctaactcgta aactagaagt
     1081 agattacgtt gacatccaac acaatttatc tacaatcgta attgtaggtg aaaatatgaa
     1141 aagtcagatt ggagtcactg caacagcgac acaagcctta tcaagagaaa aaatcaatat
     1201 caccatgata tcacaaggtt caagcgaagt ctccattatg ttcgttatag acagtaagga
     1261 tgaaaaaaga gctattaaag cactatatga aacattcttc caaaaatagt acctattaca
     1321 ctacttacac tattagatag ataacaaatc gtcctaagta agtttactta ggacgatttt
     1381 atttagaaca taggatagtt ttttcaattt taatcgtaac tacttggtat cactgacaaa
     1441 ttcggacaat taagatgcta gccgacctta aggaataata attccaataa aaaaaggcta
     1501 accaaagtta gtctccttta tctactccgc cagtaggact cgaacctacg acatcatgat
     1561 taacagtcat gcgctactac caactgagct atggcggatt at
//
LOCUS       DATRUK010000128         1579 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.130, whole genome shotgun sequence.
ACCESSION   DATRUK010000128 DATRUK010000000
VERSION     DATRUK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.130"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            218..1501
                     /locus_tag="VBE34_001955"
     CDS             218..1501
                     /locus_tag="VBE34_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO7264616.1"
                     /translation="MSLYYTEKAHAIAGPSDRQYVENPNPHIIVNVTGTDQNGNSILP
                     HYIEVNVKMGQTLSKEEILDYIARNLNSSVGGESKNVQYSNIEFKESAYLKRQLDDGK
                     TEEIAIDNDGVTVPKDGPNKFWIDVPVTCTVTPIVTETHEVRWGTPVAISHRIYFVEE
                     SSGKVLDEYTNLHTADSELNGYRVGDYITDYALSKSAYEAFLNSRLDKEGYKLQHRIS
                     TNVRQNLQINKLIFNYDFNEENIYYQIGNIRPLLSRSSAEVESDIITERYYVSKNAKS
                     LARTESTISIKMVDAKTEQPLFNHTLTGYQLVTVSHVYNRLFEENLIPTTKSGERYFI
                     QNMKKTAEQEYTVYLSETPYSKENAPVISYDARPVDWDYHSGASGSLENQPNIYTEED
                     STEFLGNKPQAACYPNKQFACENTDSKYNYSYLEK"
BASE COUNT          569 a          274 c          248 g          488 t
ORIGIN      
        1 ataaaataaa tatagatttt aaattaatat atacatttct atatttatta atttttatat
       61 cagtataact tagtgtcgta gagattaatt cctatcattc actgctttat tttaggataa
      121 ctaaacaatt tattgtttaa aatgaaagga tttatgaatg tctaggaaaa catttaaacg
      181 tatactatcg attggagttt gcacgctcgt actatcgatg agtctttatt acactgaaaa
      241 agcccacgct attgctggtc ctagtgaccg ccaatacgta gaaaacccaa atcctcacat
      301 tattgtaaat gttacaggta ctgatcaaaa cggaaatagc attttaccgc attatatcga
      361 agtcaatgta aagatgggac aaactttaag taaagaagaa attctagatt atattgctcg
      421 aaatttaaac tctagtgttg gaggagaaag taaaaacgtt caatacagca acatcgagtt
      481 taaggaaagt gcttatctga agcgtcaatt agatgatggc aagacagaag aaatagcaat
      541 tgataacgac ggtgttactg tacctaaaga cggtccaaac aaattttgga ttgacgttcc
      601 agtaacttgt actgttactc ctatcgtaac agaaacacat gaagttcgat gggggactcc
      661 agtcgctata tcacaccgta tttactttgt tgaagaatct tctggaaaag ttttagatga
      721 atacacaaat ctacacactg ctgattcgga acttaacggt tatcgtgttg gagattatat
      781 cacagactat gcactttcta agtctgctta cgaagctttt ttaaactctc gtttagataa
      841 agaaggttac aaacttcaac atcgtattag cacgaatgta cgacaaaacc ttcaaattaa
      901 taaattgatt ttcaattatg actttaatga ggaaaatatt tactaccaaa tcgggaatat
      961 ccgtccacta ttaagtcgct catcagctga agtagaatct gacatcatta cagaacgcta
     1021 ctatgtttct aaaaatgcta aaagtttagc acgtacagaa tcaaccattt cgattaaaat
     1081 ggttgatgcc aaaactgaac aaccgctatt taaccacaca ttaactggtt atcaattggt
     1141 aactgtctcc catgtctata acagactctt tgaagaaaat cttatcccaa ctacaaaatc
     1201 aggagaaaga tattttattc aaaatatgaa aaaaacagct gaacaagaat atactgttta
     1261 tctttcagaa acaccttatt ctaaagagaa cgctcccgta atttcttatg atgcaagacc
     1321 tgttgattgg gattatcact caggcgcttc aggatcactt gaaaatcagc ctaacatcta
     1381 tactgaagaa gattcaactg aatttttggg taataaacca caagcagctt gttatccaaa
     1441 caaacaattt gcttgcgaaa atactgactc taaatacaac tatagctatt tagaaaaata
     1501 gttataaccg atagggttaa tctctcatta tcttatactg attaaaatca ttaaacttgt
     1561 ttccattttg actggaaac
//
LOCUS       DATRUK010000129         1579 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.131, whole genome shotgun sequence.
ACCESSION   DATRUK010000129 DATRUK010000000
VERSION     DATRUK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.131"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..371)
                     /locus_tag="VBE34_001956"
     CDS             complement(<1..371)
                     /locus_tag="VBE34_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7264617.1"
                     /translation="MRDFEELFDKVKPIVMKLRRNYFVQLWEYDDWIQEGRIVLFRLL
                     EEHPYLLDNESKLFIYFKTKFSNYLNDVLRHQDCQKRQFNKMPYEEISEVSHYVKSKG
                     LVLDDYIAYRDTLTKVEETLS"
     gene            complement(571..990)
                     /locus_tag="VBE34_001957"
     CDS             complement(571..990)
                     /locus_tag="VBE34_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HEO7264618.1"
                     /translation="MKMTVYFDGNFWLGLIEYDDDGDYKVFRYFFGKEPKDDDVFNFI
                     NHKLNDLIKKYEFVKTDISLKRTNEHKKSPKRMQREINREKRKPVVSTKAQLAMKTIH
                     MSIKNERQLSQKCKKNELRKHRYQLKQEKRYQKKKGH"
     gene            complement(1210..>1579)
                     /locus_tag="VBE34_001958"
     CDS             complement(1210..>1579)
                     /locus_tag="VBE34_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEO7264619.1"
                     /translation="KNISYEETVVLEQQLKDNYPQYDWELKNEFYMNTHSPNTPKYTS
                     SKDAISKFPEKCTAVGERLDDRNQLELYLPYGEEEILNFIVSPTPYFEEDLLRYNVYL
                     KRVDKKKWNNIWPRLTIIKK"
BASE COUNT          506 a          292 c          194 g          587 t
ORIGIN      
        1 tctgacaaag tttcttccac ttttgttaaa gtatcacgat aagcaatata gtcatctaaa
       61 actagtcctt tactcttaac ataatgacta acttcactga tttcttcata aggcatttta
      121 ttaaattgtc gcttttgaca atcttggtgt cttaatacat cgtttaaata atttgaaaat
      181 tttgttttaa agtaaatgaa tagtttgctt tcattatcca ataaatacgg atgctcttca
      241 agcaacctaa aaagtacaat acgtccttct tgaatccaat catcatattc ccaaagttga
      301 acaaaataat ttcttctcag cttcattacg attggcttta ctttatcaaa caactcctca
      361 aaatctctca aaacaactta ttcctttctt aaacatatac taagtataaa aaaagtaagg
      421 tgataacaac atgacaatgg tgtcctttaa ggaaatgatt ttttcccaaa acacattaat
      481 tgagcattat cagactattt agtaactcat attagttaac atataaaaag cttgagatga
      541 tactctcaag ctttttatat gatgacacaa tcaatgccct tttttctttt ggtaacgttt
      601 ttcttgtttt aattggtacc gatgctttct taactcattt ttcttacatt tctgacttaa
      661 ttgacgttcg ttttttatac tcatatgaat agttttcatc gccaattgcg cttttgtaga
      721 tactactggc ttacgttttt ctctattaat ttctctttgc attcttttag gacttttttt
      781 gtgttcattc gtacgcttta gagaaatatc cgttttaaca aactcgtatt tttttatcaa
      841 atcattaagt ttatgattta taaaattaaa aacatcatca tctttaggtt cttttccaaa
      901 aaaataccga aaaactttgt aatcaccatc atcatcgtac tcaatcaaac ccaaccaaaa
      961 attaccatca aaatagactg tcattttcat acagccccct cctttaaata ttaaattgca
     1021 atggacgacc tggagggaag gttactgacg ttcactgaac gcttgcagac taccaactgc
     1081 aacgtgtttt tatgctataa cgattataac acagtacact atgattatcg ataaacgacc
     1141 ttattaaagg tctatactat ttgtcagtct aaaaatttaa aagctgttaa gccaactcca
     1201 gtaacgttat cattttttta tgattgttaa acgaggccaa atattattcc atttcttctt
     1261 atccactcgc ttcaaataaa cattataacg taatagatcc tcttcaaaat atggcgtagg
     1321 actaacaatg aaatttaata tttcctcttc accataaggg aggtaaagtt cgagttgatt
     1381 tctatcatct aacctttctc ccactgcagt acacttttca ggaaatttcg aaatagcatc
     1441 tttagagcta gtatactttg gggtattagg agaatgagtg ttcatgtaaa attcattttt
     1501 aagttcccaa tcgtactgtg gatagttatc ctttaattgt tgttctaata ccaccgtttc
     1561 ttcataagaa atattttta
//
LOCUS       DATRUK010000130         1539 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.132, whole genome shotgun sequence.
ACCESSION   DATRUK010000130 DATRUK010000000
VERSION     DATRUK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.132"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(90..1451)
                     /gene="dnaA"
                     /locus_tag="VBE34_001959"
     CDS             complement(90..1451)
                     /gene="dnaA"
                     /locus_tag="VBE34_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEO7264620.1"
                     /translation="MTENEQLFWNRVLELSRSQIAPAAYEFFVLEARLLKIEHQTAVI
                     TLDNIEMKKLFWEQNLGPVILTAGFEIFNAEITANYVSNDLHLQETSFSNYQQSSNEV
                     NTLPIRKIDSNLKEKYTFANFVQGDENRWAVSASIAVADSPGTTYNPLFIWGGPGLGK
                     THLLNAIGNQVLRDNPNARVLYITAENFINEFVSHIRLDSMEELKEKFRNLDLLLIDD
                     IQSLAKKTLGGTQEEFFNTFNALHTNDKQIVLTSDRNPNQLNDLEERLVTRFSWGLPV
                     NITPPDFETRVAILTNKIQEYPYDFPQDTIEYLAGEFDSNVRELEGALKNISLVADFK
                     HAKTITVDIAAEAIRARKNDGPIVTVIPIEEIQIQVGKFYGVTVKEIKATKRTQDIVL
                     ARQVAMYLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMVAQDDNLRIEIETIKN
                     KIR"
BASE COUNT          455 a          272 c          260 g          552 t
ORIGIN      
        1 agtattataa ctttagtatt cataaggaaa ataacttgtt cacaacctgt ggaaaagtct
       61 cgtgtttttc tgatacttat ccacaaagcc tacctgattt tatttttgat agtttctatc
      121 tcaattcgta agttgtcatc ttgagcaacc atatttttta ttttattata agcgtggaga
      181 acagttgagt gatctcgtcc cccaaattct ttacctattt tggggagact gttatctgtc
      241 atctcacgag ctaagtacat ggctacctgt cttgcaagga caatatcttg tgttctttta
      301 gttgctttta tctcttttac agttacgcca tagaatttac caacttgtat ttgaatttct
      361 tctataggaa taacagtaac aataggaccg tcatttttac gtgctctgat agcttctgca
      421 gctatatcta ctgtaatagt tttagcatgc ttaaagtcag caactagact aatatttttc
      481 aaggctcctt ctaattcacg tacgttggaa tcaaattctc ctgctaagta ttcaatggta
      541 tcttgaggaa aatcataagg atattcttga attttattgg ttaaaatagc aactcgtgtt
      601 tcaaaatcag gtggtgtgat atttactggg agtccccaac taaagcgcgt gactagacgt
      661 tcttctagat catttaattg atttggattt cggtcactgg tcaatacgat ttgtttatcg
      721 tttgtatgta aagcattgaa agtattgaag aactcctctt gggtcccccc taaggttttc
      781 ttagcaagcg actgaatatc atcaatcagg agtaagtcca agttgcgaaa cttttctttt
      841 aattcttcca tcgaatctaa acgaatatga ctgacaaatt cattaataaa attctcagca
      901 gtgatgtata aaaccctcgc gtttggatta tctcttaaaa cttgatttcc aatagcattt
      961 agtagatgcg tctttcctag accaggtccc ccccagataa atagaggatt ataagtcgtg
     1021 ccaggactat cagctacagc aattgatgct gaaacagccc atctattttc atctccttga
     1081 acaaaattag caaaagtata tttctcttta aggttagagt cgatttttct aattggtaaa
     1141 gtatttactt cgttgctaga ttgctggtag ttagaaaaac tagtttcttg taaatgtaaa
     1201 tcgtttgaga catagttagc tgtaatttca gcattgaaaa tttcaaaacc agctgttagg
     1261 ataacaggcc ccaaattttg ttcccagaat agctttttca tttcaatgtt atctaaagta
     1321 ataactgcag tttgatgttc aattttgagg agtctagcct ctagaacaaa aaattcataa
     1381 gctgctggtg ctatttgaga acgagatagc tctagtactc tattccaaaa tagttgttcg
     1441 ttttccgtca tagcttcttc cttattgtct tgtggataat tattgttata ttgtaccata
     1501 aaaaaataaa gttttccaca gatgttgaag aataaaatc
//
LOCUS       DATRUK010000131         1396 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.133, whole genome shotgun sequence.
ACCESSION   DATRUK010000131 DATRUK010000000
VERSION     DATRUK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.133"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            212..1288
                     /locus_tag="VBE34_001960"
     CDS             212..1288
                     /locus_tag="VBE34_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7264621.1"
                     /translation="MLGENIYLQRTQIGMTQENLSDYLHLTKTTISKWENNQAKPDID
                     YLILMANLFDISLDDLVGYQKTLSDDQRNQLIKDLKIKANVLSERDFFQEVKELSKQF
                     PNDFKTLLIMINMVLSNLTNLNDSEMKEWSLSTLNKIISKTVVESDLQTAIMFKTVVL
                     FHNLEYDEVINLYQNSPYKLGEELLLANSFFAKGDVYRAKQVLQVEIYQQILLVCEYL
                     LSLSTFEVEENKGDIFERLEHMIEAFQLNSLHPNTVIKCYYSLACHYSKSDDDKALGY
                     LKQCFKSLKQLLQRFELHGDTFFYTIDDWLETIPTGIAPPTSQLQVIERVEDLFQNSQ
                     FKELSGRKEFQQIIQKLNDLKKDY"
BASE COUNT          497 a          198 c          230 g          471 t
ORIGIN      
        1 ttaagggctc ttttataatt cattattttt caatgacaaa tattagtgaa gttaattttt
       61 atttaaccat tgttattatt aatgctgttt tgccacttat taatcaacga aaaagtttct
      121 tctatggaaa cttttttata ttatatttcc tgataggaac ttttattttt gatttagaaa
      181 ctctataatg gacgtgaaag gagattgacc gatgcttgga gagaatatct atttacaacg
      241 tactcagatt ggtatgacgc aagagaattt gagtgattat ttgcatttaa caaaaactac
      301 aatttcaaag tgggaaaata atcaagctaa accggatatt gactatctta ttttgatggc
      361 taatcttttt gatattagct tggatgactt agtcggttat caaaaaacat tgtcagatga
      421 tcaaagaaat cagctcatta aggatctaaa aataaaagca aatgtattgt ctgagagaga
      481 tttctttcag gaagttaagg aattatcaaa acagttccct aacgatttta agactctgtt
      541 gataatgatt aatatggtct tgagcaacct aaccaacctt aatgattcag aaatgaaaga
      601 gtggtcacta agtacactaa ataaaattat tagtaaaaca gtagttgagt ctgacctcca
      661 gacggcgata atgttcaaaa cggtcgttct ttttcacaac cttgaatatg atgaagtgat
      721 aaacctttac cagaatagtc cctataaatt aggtgaagaa ttacttttag ctaatagctt
      781 ttttgcaaaa ggcgatgttt accgagctaa gcaagtatta caggttgaaa tttatcagca
      841 gattctactt gtatgcgagt atctattgag tttatcaact tttgaagttg aagaaaacaa
      901 aggcgatatt tttgaaaggc ttgaacatat gatagaagcg tttcaattaa actctttgca
      961 tcccaacaca gtcatcaagt gttattatag tttagcttgt cattatagta aaagtgatga
     1021 tgataaggca ttaggatatt taaaacaatg ttttaaatct ttaaaacagt tgctccaaag
     1081 atttgaatta cacggtgata ctttctttta taccatcgat gattggttag agacaatacc
     1141 tactggtatt gcaccaccga cgagtcaatt acaggtaata gaacgagttg aggacttatt
     1201 tcaaaattca caatttaaag aactatcagg gcgtaaagaa tttcaacaaa ttattcaaaa
     1261 acttaacgat ttaaaaaagg attactaata tgaatacaga aataccaaat caattattac
     1321 cattcctagt tccgttgatt atgttgcaag gagctttaat tatcatttct ctagtcaaac
     1381 taagtaaact taagat
//
LOCUS       DATRUK010000132         1365 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.134, whole genome shotgun sequence.
ACCESSION   DATRUK010000132 DATRUK010000000
VERSION     DATRUK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.134"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..1304)
                     /gene="brnQ"
                     /locus_tag="VBE34_001961"
     CDS             complement(<1..1304)
                     /gene="brnQ"
                     /locus_tag="VBE34_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO7264622.1"
                     /translation="MVKKGFLTGLLLFGIFFGAGNLIFPPALGVASGQDFWPAILGFC
                     LSGVGLAIITLLLGTLTNGGYKTEMSEKFSPWFSLTFLVILYLTIGPLFAIPRTATVS
                     FEIGVAPIVGHSPIALLCFTACFFAAAYYLAIRPNGILDSVGKILTPVFAFLILSLVV
                     VGAIAYGNLESAKASADYAGKAFGSGVLAGYNTLDALAAVAFCLVATETLKKFGFKTK
                     KEYLSTIWIVGIVTSLAFSILYIGLGFLGNKFPVPADILADPNVNKGAYVLSQASYKL
                     FGNFGRYFLSIMVTLTCFTTTVGLIVSVSEFFDKNFRFGNYKLFATVFTLIGFLIANL
                     GLNAVITFSVPVLTLLYPIVIVIVLIILINKWLPLSKKGMSLTIGLVTLVSFVEVLAG
                     QWQEKTLTQLVGFLPFHTISMGWLVPMLIGIVFSLVLSDKQKG"
BASE COUNT          535 a          279 c          194 g          357 t
ORIGIN      
        1 cctttttgtt tatctgacag caccaaagaa aatactatac caatcaacat tggaactagc
       61 caccccattg aaatagtatg aaaggggagg aatccaacta actgagttaa tgttttttct
      121 tgccattgtc ccgctagaac ctcaacaaac gacactaaag tgacaagacc aattgtcaaa
      181 gacattccct tttttgaaag cggcaaccac ttattaatca aaataattaa gacaataaca
      241 ataacaatcg gatacaataa agttagcaca ggaactgaaa aagtaattac agcatttaaa
      301 ccaagattag caattaaaaa gccaatcaaa gtaaaaacag tagcaaataa tttataattc
      361 ccaaaacgaa agttcttatc aaaaaattca gatactgaca caataagccc aactgttgtt
      421 gtaaagcatg tcaatgttac cataatactc aagaaataac gaccaaaatt tccaaataat
      481 ttataagatg cttgtgataa tacataagct cctttattaa catttgggtc agctaaaata
      541 tcagctggga caggaaactt attccctaaa aaacctaagc caatatataa aatactaaat
      601 gctaaactag taacaattcc aacaatccaa atggttgata aatattcttt tttagtttta
      661 aaaccaaact ttttaagcgt ttctgttgct actagacaaa aagcaactgc tgcaagtgca
      721 tcaagtgtat tatagcctgc taagacacca cttccaaacg cttttcctgc atagtcagca
      781 ctcgccttag cactctccaa gtttccataa gcaatagctc caacaacaac caaagagaga
      841 attaaaaagg caaaaacagg cgttaagatt tttccaacac tatctaatat accatttggt
      901 ctaatagcga gataataagc tgcagcaaaa aaacaggctg taaaacacaa aagagcaatc
      961 ggactatgcc ctacaattgg agcaacccca atttcaaaag aaaccgttgc tgttctagga
     1021 atagcaaaaa gtggaccaat agttaggtag agtataacta aaaatgttaa agaaaaccac
     1081 ggactaaatt tttccgacat ctctgtctta taaccaccat tagttagtgt gcctagtagg
     1141 agggtaataa tagctaatcc aactcctgac aaacaaaatc ctaaaatcgc tggccagaaa
     1201 tcttgtccag atgctacacc taatgctggt ggaaaaatga ggttccctgc accaaagaag
     1261 ataccaaata agagtaatcc cgttaaaaag ccttttttta ccataagcaa ccattagtgt
     1321 aaataatcta tttacaccct ttccataaat tctccttatc aaatt
//
LOCUS       DATRUK010000133         1344 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.135, whole genome shotgun sequence.
ACCESSION   DATRUK010000133 DATRUK010000000
VERSION     DATRUK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.135"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            287..1309
                     /locus_tag="VBE34_001962"
     CDS             287..1309
                     /locus_tag="VBE34_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7264623.1"
                     /translation="MNIIIGTSLLILVLAIFTLFNYKAPYGTKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVTIAIGVTPGYAVLIGLSVSGTG
                     IIPGFLAGYLVGFLVKWMERNIPGGLDLISIIIIGAPLTRLVAKLLTPLINSTLLTIG
                     DILTSGAHSNPILMGVILGGTIVVVATAPLSSMALTAMLGLTGMPMAIGALSVFGSSF
                     MNGVLFHKLKLGSRKDNIAFAVEPLTQADVTSANPIPIYVTNFVGGAACGILIALMKL
                     VNDTPGTATPIAGFAVMFAYNPMIKVLITALGCIILSLLAGYFGGIVFKDYKLVTKEE
                     LQARDN"
BASE COUNT          397 a          211 c          264 g          472 t
ORIGIN      
        1 aaagattaat gtaataaaaa attatcgtaa taaaaaaaag ttaagaaaat aaaaaattat
       61 tattgactat ggttatgctt ttaggtaaaa tgtgattata ttagcaacta aggagtaatc
      121 catgagataa gcctaataat tatgtcggac ttctttgttg caccttatcc agttcgtagt
      181 acaattaagt ttcagtatta cctaaaggcg cctttattga aattgaagca gtagtacaca
      241 gacactgaga ataaaaaact taactatttt tagcgggagt atatttatga atatcattat
      301 tgggaccagt cttttaatac ttgtattagc gattttcact ttatttaatt ataaagcacc
      361 ctacggaaca aaagcaatgg gagcattggc atctgcggct tgtgcatcct tccttgtaga
      421 agcttttcaa gattctttct ttggtaaagt tttgggattt caatttttaa gtgaagtggg
      481 tggtgctaat ggttcacttt ctggtgtagc tgcagctata ttggttacta ttgctattgg
      541 tgtaacccca ggatacgcag ttttaattgg tttatctgtt tctggaacag gtattattcc
      601 tggtttcctt gcaggatatt tagtgggatt tcttgttaag tggatggaaa gaaatattcc
      661 tggtggttta gatcttattt caattattat tattggtgca ccattaacac gtcttgtggc
      721 caaactgttg acgccgctta ttaatagcac tttgctcacc attggagata tcttaacatc
      781 aggtgctcat agcaacccta ttttaatggg ggttatactt ggcgggacaa ttgtagtagt
      841 ggcgacagca ccactttctt ctatggcatt gacagctatg ctaggattaa ccggaatgcc
      901 tatggctata ggagccttgt ctgtctttgg ttcgtcattt atgaatggtg tacttttcca
      961 taaattaaaa cttggaagtc gtaaagataa tatagctttt gctgttgagc ctctaactca
     1021 agctgacgtg acttcagcta accctattcc aatctatgtc actaattttg ttggtggtgc
     1081 agcttgtggt attttaattg ccttgatgaa attagttaat gatactcctg gaacagcgac
     1141 accaattgca ggatttgctg tcatgtttgc ctataaccca atgataaaag tactaataac
     1201 cgctctaggt tgtattatcc tatctttact agcaggctat tttggaggca ttgtttttaa
     1261 agattataaa ttagttacaa aagaagagct acaggctaga gataactagt tacttcatga
     1321 aaagctaata gtaaatatca ttaa
//
LOCUS       DATRUK010000134         1222 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.136, whole genome shotgun sequence.
ACCESSION   DATRUK010000134 DATRUK010000000
VERSION     DATRUK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.136"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            144..1049
                     /locus_tag="VBE34_001963"
     CDS             144..1049
                     /locus_tag="VBE34_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7264624.1"
                     /translation="MKNQQKLMYLESIELYSNITKAAAHLFISQPYLSKVIKQLENEL
                     EIKLIQSQGHQTFLTYAGQRYLFYLKEIDMIERQMAKELYLIRSDKKGEITLGINSGL
                     ASSILANVLPKFNLEHPEISVKLLENNQNISEQLVASGDIDLAVGMAPILYKDGIAST
                     TIYRDELFLMIPTTSQLYNAEKRGQIIPFEYPISVLDNEPLILTPLEYGIGKTIAQFY
                     ELHHMSLNQMITTSTVPTAASLSLSGMGATFVPQTLIHRYLDKECNVYHFHKNKLFSE
                     YIMIYKKDVELSGIALLLYKAFLTK"
     gene            1110..>1222
                     /locus_tag="VBE34_001964"
     CDS             1110..>1222
                     /locus_tag="VBE34_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEO7264625.1"
                     /translation="MIYKIIASLFLVLIPIFSQVLVKIFKLKKFNIMFPDVA"
BASE COUNT          428 a          225 c          172 g          397 t
ORIGIN      
        1 tctataacta aatagttatg aatatatcat gttatactac taaattaaat agattgattt
       61 tagctcaaaa tgatagtaaa cttatagcca taacgtatca taacttatta ttaatctaag
      121 gatatcaaac tggttataaa gtcttgaaaa atcaacaaaa attaatgtat ctagagagta
      181 tcgagctata ctcaaacatc actaaagccg ctgcacacct ttttatttct caaccttacc
      241 taagcaaagt gatcaagcaa ttagaaaatg agttagaaat aaaactcatc cagagccaag
      301 gacaccaaac ctttttaact tatgctggac agagatatct attctaccta aaagaaattg
      361 atatgattga gcgtcaaatg gccaaagaac tatatctcat ccgttctgat aaaaaaggag
      421 aaatcacact tgggattaac tctggcttag caagttctat tctggcaaat gtacttccca
      481 aatttaactt ggagcaccca gaaatttctg tcaaattact cgaaaacaat caaaatattt
      541 ctgaacaatt ggtagcgagt ggtgatattg acctagctgt tgggatggct cctatccttt
      601 ataaagatgg aattgcatca accactattt accgcgacga attgttttta atgataccta
      661 ccaccagcca actctacaac gctgaaaaac gtggacagat tattcctttt gaatatccta
      721 tttcagttct agacaatgaa cctcttatcc taacaccatt agagtacgga attggcaaaa
      781 caattgcaca attctatgag cttcatcaca tgtcattaaa tcagatgatt acaactagta
      841 ccgttcctac agcagctagc ctatctttgt cagggatggg agcaacattt gttccacaaa
      901 ctctcattca tcgctatttg gataaagagt gcaatgtcta ccattttcat aagaataagt
      961 tattttcaga gtacattatg atttataaaa aagatgttga attatctgga attgctctcc
     1021 tactttataa agctttttta accaagtagt ttcgctaatt ctattgaaaa tcagtttact
     1081 tcttaccaaa atcggtataa aatagaccta tgatatacaa aattattgct tctttatttc
     1141 ttgttttaat ccctatattt tcacaggttc ttgtcaaaat atttaaactt aaaaaattta
     1201 acattatgtt tccagatgtt gc
//
LOCUS       DATRUK010000135         1142 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.137, whole genome shotgun sequence.
ACCESSION   DATRUK010000135 DATRUK010000000
VERSION     DATRUK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.137"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..154)
                     /locus_tag="VBE34_001965"
     CDS             complement(<1..154)
                     /locus_tag="VBE34_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264626.1"
                     /translation="MKKKLLGLMILAISTVFLVACSTNSLNVKYYKVYDGEKMLVLEI
                     NDESGIY"
     gene            complement(307..495)
                     /locus_tag="VBE34_001966"
     CDS             complement(307..495)
                     /locus_tag="VBE34_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, toxin component, MazF
                     family protein"
                     /protein_id="HEO7264627.1"
                     /translation="MSDIEVYSVLVSRIEYSDGTGSKVRPAVVVKFNDEVIKTLRFTT
                     KYENKSDNIRSQYLEVID"
     gene            complement(492..785)
                     /locus_tag="VBE34_001967"
     CDS             complement(492..785)
                     /locus_tag="VBE34_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEO7264628.1"
                     /translation="MSTVAVRVDDQLKDDATELFQSLGLDMSTAVKMFLIQSVKTQSI
                     PFEIKNKSSVSDEEFQNLVETKLKGIRVKASDPESVNAFFGDEDFSEYEEYFK"
     gene            842..991
                     /locus_tag="VBE34_001968"
                     /pseudo
     CDS             842..991
                     /locus_tag="VBE34_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          406 a          225 c          105 g          406 t
ORIGIN      
        1 gataaattcc tgattcatca ttaatttcta aaactaacat tttttcacca tcatatactt
       61 tataatactt tacatttaac gaattagtcg aacaagcaac taaaaatact gtagatatag
      121 ctaaaatcat caatcctaat aatttctttt tcataactaa acttcctttg ctatgagaaa
      181 atcttttaac ctctcaacat ctctatcgct taacttacct ataattttaa tattgaaccc
      241 tttattctca acatcataat attgaatagt atctatccaa gaacgcctat ttaaatttgc
      301 ttttgcctaa tcaataactt ctaaatactg acttcttata ttgtcagact tattttcata
      361 tttagtagta aatcttaaag tttttattac ttcatcatta aatttgacta ctacagcagg
      421 acgaaccttt gaacctgtac catcagaata ttcaatacga gaaactaata cagaatatac
      481 ctcaatatca ctcatttaaa atattcctca tattctgaaa aatcttcatc tccaaaaaac
      541 gcatttacac tttctggatc actagcctta actcgtattc cctttaattt tgtttctacc
      601 aaattttgaa attcttcatc agaaacagat gatttatttt ttatttcaaa aggaatactt
      661 tgagttttaa cagattgaat caaaaacatt tttactgctg tgctcatatc caaccctaag
      721 gattgaaaaa gttctgtagc atcatctttt aattgatcat caactcttac tgctactgtt
      781 gacataatat cacctcaaat catttttgta taactattgt atcactaaat gcaatacaac
      841 gcaaacaaaa aaggaaatcg tctttcccaa tgaagaggct ctagaatact atctagtcac
      901 actatttaat gattacaact tcaagcaaga acaacgaatc cacaaaggtt ttggccaatg
      961 tacagacaca cttgaaagcc tatttgatta acaatcccag ctttgccagt gtgtttacac
     1021 aacattattg acacactcct tttattttta taaattaccc ttaaatacat ttaattactt
     1081 gaaaagacaa acagtcattt tagaaaattt tgcatctaac cttacatttg attacaccta
     1141 aa
//
LOCUS       DATRUK010000136         1110 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.138, whole genome shotgun sequence.
ACCESSION   DATRUK010000136 DATRUK010000000
VERSION     DATRUK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.138"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(1..284)
                     /locus_tag="VBE34_001969"
                     /pseudo
     CDS             complement(1..284)
                     /locus_tag="VBE34_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            462..662
                     /locus_tag="VBE34_001970"
     CDS             462..662
                     /locus_tag="VBE34_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO7264629.1"
                     /translation="MSQEKLKSKLDQAKGGAKEGFGKITGDKELEAKGFIEKTIAKGK
                     ELADDAKDAVEGAVDAVKEKLK"
     gene            704..892
                     /locus_tag="VBE34_001971"
     CDS             704..892
                     /locus_tag="VBE34_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO7264630.1"
                     /translation="MSIRTDIDEFKEAIDKGYISGNTVAIVRKNGKIFDYVLLHEEVR
                     EEEVVTVERVLDVLRKLS"
BASE COUNT          405 a          140 c          220 g          345 t
ORIGIN      
        1 tttgcatcca tacgatgact caatttccaa gcaatatatt ttctagaata gagatcaatt
       61 attgcgcaga gatagacata tttcttatat ccaatagaaa tataggtgaa gtcagtagac
      121 catacttgat taggtttatc aggattaaat ttctggttga gtaagttttg aggatacgtt
      181 ttttgagtct ttttaagagc tgtttttgtc ttaacggtag ccattttagg gagcgccata
      241 ttcttcatca gacggtaaat tcttccttca gagatttttg tgtcttttaa aacaggttat
      301 aacacacaaa gtgttgtcta tttttatagt atacatccat actcgcataa ccatgagata
      361 atcactaaaa caagagtcgc ctgacactag cgactcttgt ttttatttgc taaagaaaga
      421 tatagatgtt atgattaata aaaataataa ggaggtgcag tatgtcacaa gaaaaactaa
      481 aatcaaaatt agatcaagca aaaggtggtg ctaaagaagg ctttggcaaa ataaccggtg
      541 ataaagagtt agaagcaaaa ggatttatcg aaaaaacaat tgctaaaggc aaagaactag
      601 cagatgatgc taaagatgct gttgaagggg cagtagatgc tgtcaaagaa aaactgaagt
      661 aacaaataac cgctcattta acgagcggtt atttgttatc tttttgtcta tcagaacaga
      721 tatagatgag tttaaagaag cgattgataa aggctatatt tcagggaaca cagtagcgat
      781 agtgcgtaaa aacggaaaga tatttgatta tgtgttacta cacgaagaag tgagagaaga
      841 agaggttgtt acagttgaga gagtgcttga tgtactgagg aagttatcat aataacggac
      901 caaaattcgc cccaaaaagt attttaaaat agaataaaat aaaaaaatat gctttttatt
      961 atggatgtat cgtgtatttt aacctttaat atatcctatt ttaatggtag agaagttggc
     1021 aggggacata ttaaaacctt ttttgagatg ttaggaagga ttttattctt tagactagtg
     1081 ttgagaaaat aacaaaactt aaataagatt
//
LOCUS       DATRUK010000137          943 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.139, whole genome shotgun sequence.
ACCESSION   DATRUK010000137 DATRUK010000000
VERSION     DATRUK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.139"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            288..>943
                     /locus_tag="VBE34_001972"
     CDS             288..>943
                     /locus_tag="VBE34_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7264631.1"
                     /translation="MFNKIGFRTWKSGKLWLYMGVLGSTIILGSSPVSAMDSVGNQSQ
                     GNVLERRQRDAENKSQGNVLERRQRDAENKSQGNVLERRQRDVENKSQGNVLERRQRD
                     AENKSQGNVLERRQRDADNKSQVGQLIGKNPLFSKPTVSRENNHSSQGDSNKQSFSKK
                     VSQVTNVANRPMLTNNSRTISVINKLPKTGGDQNVIFKLVGFGLILLTSRCGLRRNEN
                     "
BASE COUNT          356 a          123 c          174 g          290 t
ORIGIN      
        1 ctagtttctg atggtttaga tgaagtatta aaaatatact attatctcat tgtaaatcct
       61 aatgttagta tgactatcta tcatgtttta taatattaaa ggaaaattta aaaatatcat
      121 gttttagata tcaactattt aattttaagc atacaaatta ataataaatt gtaattaaat
      181 aacaaattaa cttggcatag attataaaat gttttaatat atataatcta aataaaaata
      241 ataataaaat gacttttaaa atttaaaaaa agtaaggaga aaattaattg ttcaataaaa
      301 taggttttag aacttggaaa tcaggaaagc tttggcttta tatgggagtg ctaggatcaa
      361 ctattatttt aggatcaagt cctgtatctg ctatggatag tgttggaaat caaagtcaag
      421 gtaatgtttt agagcgtcgc caacgtgatg cggaaaacaa aagtcagggt aatgttttag
      481 agcgtcgcca acgtgatgcg gaaaacaaga gccaaggcaa tgttttagag cgtcgtcaac
      541 gcgatgttga gaataagagc caaggcaatg ttttagagcg tcgtcaacgt gatgcggaaa
      601 acaaaagtca gggcaatgtt ctagagcgcc gccaacgtga tgcggataac aagagccaag
      661 taggtcaact tatagggaaa aatccacttt tttcaaagcc aactgtatct agagaaaata
      721 atcactctag tcaaggtgac tctaacaaac agtcattctc taaaaaagta tctcaggtta
      781 ctaatgtagc taatagaccg atgttaacta ataattctag aacaatttca gtgataaata
      841 aattacctaa aacaggtggt gatcaaaatg tcatttttaa acttgtaggt tttggtttaa
      901 ttttgttaac aagtcgctgc ggtttgagac gcaatgaaaa tta
//
LOCUS       DATRUK010000138          910 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.140, whole genome shotgun sequence.
ACCESSION   DATRUK010000138 DATRUK010000000
VERSION     DATRUK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.140"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..>910
                     /locus_tag="VBE34_001973"
     CDS             <1..>910
                     /locus_tag="VBE34_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264632.1"
                     /translation="KDSSYSSNINISEDKIKENDQKILDLIVKSGANNQNLTDEEKVI
                     AFTKYIGEITNYDNEAYRARNVDTEYYRASDLFSVTERKLAMCVGYSVTAARAFNIMG
                     IPSYVVSGKSPQGISHAAVRAYYNRSWHIIDITASTYWKNGNYKTTYSDFIKEYCIDG
                     YDVYDPAKTNNRFKVKYMESNEAFENWIHNNGSKSMLFINESAALKDKKPKDDFVPVT
                     EKEKNELIDKYKKLLSQIPENTQNPGEKNIRDYLKNEYEEILKKDNLFEHEHAEFKES
                     LNLNESFYLQLKKEEKKPSDNLKKEE"
BASE COUNT          403 a          109 c          148 g          250 t
ORIGIN      
        1 caaaagattc ttcatattct tcaaacatta atattagtga agataaaata aaagaaaatg
       61 accaaaaaat tctagattta atagttaaat ctggagctaa taatcaaaac ttaacagatg
      121 aagaaaaagt aattgctttc actaaatata taggtgaaat aactaattat gacaatgaag
      181 cttacagagc tagaaatgtc gacacagaat attacagagc ttcagattta ttttctgtaa
      241 cagaaagaaa acttgctatg tgcgttggct atagtgtaac agccgctaga gcatttaata
      301 ttatggggat acctagttat gtcgtttctg gtaaaagtcc tcaaggaata tctcatgcag
      361 ctgtgagagc gtattataat agaagttggc atatcataga tatcacagcc agtacatatt
      421 ggaaaaatgg taattacaaa acaacgtatt ccgattttat taaagaatac tgtatagatg
      481 gttatgacgt ttatgatccc gcaaaaacaa ataatagatt taaagttaag tatatggaat
      541 caaatgaagc gtttgaaaat tggatacata ataatggaag caaatctatg ttatttatta
      601 atgaatctgc agctctaaaa gataaaaaac caaaagatga ttttgtacca gtaacagaaa
      661 aagaaaaaaa cgagctgata gataagtata aaaaattatt gtcacagatt cctgaaaaca
      721 cacaaaatcc aggagaaaaa aacataagag attatttgaa aaatgaatat gaagaaatat
      781 taaagaaaga taatttattt gagcatgaac atgcagaatt taaagaaagt ctaaatttaa
      841 atgaatcatt ctacctacaa ttaaagaaag aagaaaagaa accgagtgat aatctcaaaa
      901 aagaagaaaa
//
LOCUS       DATRUK010000139          879 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.141, whole genome shotgun sequence.
ACCESSION   DATRUK010000139 DATRUK010000000
VERSION     DATRUK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.141"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            53..586
                     /locus_tag="VBE34_001974"
     CDS             53..586
                     /locus_tag="VBE34_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEO7264633.1"
                     /translation="MRLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTENDGRRWVTREPAIVYFHKKYWFNIIAMIRETGVSYYCNLASPYILDPEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEQHKAQMNYPTDIDYILKENVKILVEWINENKGPFS
                     SSYINIWYKRYLELKKR"
     gene            complement(701..772)
                     /locus_tag="VBE34_001975"
     tRNA            complement(701..772)
                     /locus_tag="VBE34_001975"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(737..739),aa:Glu,seq:ttc)
     gene            complement(783..856)
                     /locus_tag="VBE34_001976"
     tRNA            complement(783..856)
                     /locus_tag="VBE34_001976"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(820..822),aa:Ile,seq:gat)
BASE COUNT          315 a          158 c          161 g          245 t
ORIGIN      
        1 aaaattatga taaactataa cagacgtata aattgtagaa agttggtagg ctatgagatt
       61 acctaaagaa ggcgacttta ttacaattca aagttacaaa catgatggta gtttacaccg
      121 aacttggcgt gacaccatgg tattaaaaac aaccgaaaat gccctcattg gtgttaatga
      181 tcatacttta gtaacagaaa atgatggtcg acgctgggtg acacgagagc ctgcaatagt
      241 atactttcat aaaaaatatt ggtttaacat tatcgctatg atacgtgaaa ctggtgtctc
      301 ctactattgt aatctagcaa gtccgtatat cttggatcct gaagcactca agtacatcga
      361 ctacgacctt gatgtcaaag tatttgcaga tggtgaaaaa agactactgg atgtggacga
      421 atatgaacag cataaagctc agatgaacta tcctaccgat attgattata tattaaagga
      481 aaatgtaaaa atattggtag aatggataaa tgagaataaa ggcccctttt catcatcata
      541 tatcaatatc tggtataaac ggtaccttga attgaaaaag cgctaactaa cacactaaat
      601 agtgtgtttt tattaatatc aaatttaatt acaatactat tgcaaaaata tatacttaaa
      661 ataaaaaaag tagaaagtga tctttctact tttttaagaa tagtccgtac gggattcgaa
      721 cccgtgttac cgccgtgaaa aggcggtgtc ttaacccctt gaccaacgga ccatattctt
      781 gatgggcacg agtggactcg aaccaccgac ctcacgctta tcaggcgtgc gctctaacca
      841 cctgagctac gcgcccaaaa taacttctaa aatataaag
//
LOCUS       DATRUK010000140          855 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.142, whole genome shotgun sequence.
ACCESSION   DATRUK010000140 DATRUK010000000
VERSION     DATRUK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.142"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            230..649
                     /locus_tag="VBE34_001977"
     CDS             230..649
                     /locus_tag="VBE34_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEO7264634.1"
                     /translation="MDNCIFCKIISGEISSSKVYEDDEVLAFLDITQTTTGHTLLIPK
                     KHVRNVLEMDEKTAQITFERLPKVARAVQAATKAKGMNIINNNEEIAGQTVFHAHVHL
                     VPRFDESDGIKIHYTTHEPDFEALAKLAKEIRKEIQL"
     gene            646..>855
                     /locus_tag="VBE34_001978"
     CDS             646..>855
                     /locus_tag="VBE34_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264635.1"
                     /translation="MKIGRLIALGLVSLGALELYKNRKTIKDSYQNTKNETDSAKLKL
                     ERIKNDLAIISQEKEKIRLISQELNH"
BASE COUNT          327 a          149 c          129 g          250 t
ORIGIN      
        1 ttttagatac ttccaaaatt ctagaaatgt acttactcaa taataaacat cattaaaaat
       61 tgcaataccc tttaggtcat gctaagataa gtatgatggt aaatatagat atcacaatca
      121 atacaggcta cttttatcca cctgtacgac ttatgaccta tcaaacgata aaaatttacc
      181 attccttaat agctacccaa taaatttttc aacatgaaag gaattagtta tggataattg
      241 tattttttgt aaaatcatca gtggagaaat ctcttcatca aaagtttatg aagatgatga
      301 agtattagct tttctcgaca ttacacaaac tacgactggg cacacccttc ttattcctaa
      361 aaaacatgtt cgaaatgtgt tagagatgga tgaaaaaact gcacagataa cctttgaacg
      421 cttgcctaaa gtggcgcgtg ctgtccaagc tgctacaaaa gccaaaggaa tgaatattat
      481 taacaacaat gaggaaattg ctggacaaac tgtttttcat gcacacgtcc accttgttcc
      541 ccgctttgat gaatcagacg gtatcaaaat tcactacact actcatgaac ccgactttga
      601 ggcacttgca aaattagcca aagaaattag aaaagagatt caattatgaa aataggtcgt
      661 ttaattgcac taggtttagt tagtttagga gcgcttgaac tttacaaaaa tagaaaaact
      721 atcaaggatt cctatcaaaa tacaaaaaat gagacagatt cagctaaatt aaaattagaa
      781 cgtatcaaaa atgatcttgc tattattagt caggaaaaag aaaaaatcag attaatttct
      841 caagaactca atcat
//
LOCUS       DATRUK010000141          849 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.143, whole genome shotgun sequence.
ACCESSION   DATRUK010000141 DATRUK010000000
VERSION     DATRUK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.143"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..273)
                     /locus_tag="VBE34_001979"
     CDS             complement(<1..273)
                     /locus_tag="VBE34_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEO7264636.1"
                     /translation="MSTFIVYIHLSERLEEFKTEAFDFKTRASYVTAKLFFLGNMIKH
                     NTNSSKELIRSLKNDKSVLAMIPHKELFNRSTLDKKSLSYMMSDKEL"
     gene            327..849
                     /locus_tag="VBE34_001980"
                     /pseudo
     CDS             327..849
                     /locus_tag="VBE34_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          311 a          150 c          132 g          256 t
ORIGIN      
        1 taattcttta tcagacatca tataagatag agactttttg tccaatgttg agcggttaaa
       61 taactcttta tgaggaatca ttgcaagaac tgatttatca ttctttagtg atcttattaa
      121 ttcttttgaa gagttggtat tatgctttat catattacct aaaaagaata attttgctgt
      181 gacataactt gctcgagttt taaagtcaaa tgcctctgtt ttaaattctt caagtctttc
      241 agaaagatgg atgtatacta taaaagtaga caaaactttg tgtgttacaa tctgttttaa
      301 aagacacaaa aacgaaagga caacctatat caacatttaa acgctatgat gaagaattca
      361 aacaatctct tgttaacctt tatcaaactg gaaaaactca gtctgaactc tgtaaagact
      421 atggggtatc cacttctacg cttgcaaaat ggatcaaaca gtattcccaa gtcaaactcg
      481 aagataattc tgtacttact gccaagcaaa tacaagagtt acaaaaacgt aatgcgcagc
      541 ttgaagagga gaaccttatc ctaaaaaaag caagtgccat attcatgcaa aacttaaagt
      601 aagactcctc gctgtctatc ggttacgctt tgaacacgcc acaacaacct tgtgtcgtgt
      661 tttacgtgtc aatcgctcca cttactataa atttctaaaa cataagccct caaaaagaga
      721 attggataat caaatttata gaaaacaaat acttgagatt tataccaaag cgaacaaaag
      781 acttggtgtg aagtctatca aggtcattct tcaaagagac tatgacacaa aaatctctga
      841 aggaagaat
//
LOCUS       DATRUK010000142          803 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.144, whole genome shotgun sequence.
ACCESSION   DATRUK010000142 DATRUK010000000
VERSION     DATRUK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.144"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            112..>803
                     /locus_tag="VBE34_001981"
     CDS             112..>803
                     /locus_tag="VBE34_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264637.1"
                     /translation="MLNSDFSISKSLANYIHHRRLEVGVSSTDLAEISNMSKSDWESF
                     EKNGGAIPLKSKDIILDLLFLERFPKEKECDFIDKLFEEAKENKLWPEKIYQTMGLTP
                     ALSFIAGCEILSDDINNDLEELSKLPKESHLGQLDTSLLLSLLPQQFITKYDYEFVYK
                     LSKVLAQYTSRNKVGSSYTAHSVIEEIYLYLIAKESILYFESLDENSHLQLKELLDYN
                     DEWPFDIFDDMD"
BASE COUNT          282 a          108 c          138 g          275 t
ORIGIN      
        1 acatctctag aaaatcatga tgtgctaaat caaaagatgc atgtgttttt acttgaaaaa
       61 aattttttat atgtgataat cataatgaat aggaatcaaa aggagaacgg aatgctaaat
      121 tctgatttta gtattagtaa gtcacttgct aactacattc accatcgtag gttagaagtt
      181 ggcgtgtcgt caactgattt ggcagaaata tccaatatgt ctaaatcaga ttgggaatca
      241 tttgaaaaaa atggtggggc aattcctttg aaatctaagg atatcatact tgacttatta
      301 tttttggaga gattccctaa agagaaagaa tgtgatttta tagataaatt atttgaggaa
      361 gcaaaggaaa ataaactttg gcctgaaaag atttaccaga ctatgggact tactcctgct
      421 ctttcattta ttgctggttg tgaaatactt tctgatgata ttaataatga tttggaggaa
      481 ttatctaaac tacctaaaga aagtcatctg ggacaattag atacttccct acttttatca
      541 ctattgcctc aacaatttat cacaaaatat gattatgagt ttgtttataa attaagcaaa
      601 gttttggctc aatatacatc aagaaataaa gttggttcat cttatacagc gcatagtgta
      661 atagaggaaa tttatctcta tttaattgca aaagaatcca ttctttattt tgaatctctg
      721 gacgaaaact cccatctaca acttaaggaa ttattagatt ataacgatga gtggcctttt
      781 gatatttttg atgatatgga tag
//
LOCUS       DATRUK010000143          794 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.145, whole genome shotgun sequence.
ACCESSION   DATRUK010000143 DATRUK010000000
VERSION     DATRUK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.145"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            32..>794
                     /locus_tag="VBE34_001982"
     CDS             32..>794
                     /locus_tag="VBE34_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEO7264638.1"
                     /translation="MKLRKQLAQQIVTSIKDVCQQDINFINTKGIIFASTNPKRVGEF
                     HEIGLKVAQTGQMIEVTDQESYFGTQAGINIPFYYNCELLATIGISGNPNQVGKYALL
                     AQKMTRLILKEHELDYLDFGRKNEASIVLHHLVEGRELDYYYLNQFLNQYHLSEKTDY
                     RLLTFEINSQKQKLLLSQSEMSLLNFFDKLDTAIYTFNYPNQYWLLLSDQMFDYYYPN
                     ILSKFECEKGLYKVGIGQKSSLSLLKRSYETSILAL"
BASE COUNT          290 a          140 c          114 g          250 t
ORIGIN      
        1 tatttgttat atataacaaa aggtaagctg aatgaaatta agaaaacaac tcgcacaaca
       61 aatcgttacc tctatcaaag atgtttgtca acaagatatt aattttatta ataccaaagg
      121 aatcattttc gctagtacca atccaaaacg tgttggagaa ttccatgaaa ttggtttaaa
      181 agtagcacag acaggacaaa tgattgaagt aacagatcaa gaatcttatt ttgggacaca
      241 agcagggatc aatattcctt tctattataa ttgtgaatta ttagctacta ttgggattag
      301 tggaaacccc aaccaagtag gaaaatatgc tttacttgct caaaaaatga ctcgactcat
      361 cttaaaagag catgaattag attatctgga tttcgggcga aaaaacgaag cttctattgt
      421 cttacaccat cttgttgaag gcagagagtt agactactat tacctcaatc aatttttaaa
      481 ccaataccac ctatcagaaa aaactgacta tcgtctactc acttttgaaa tcaatagtca
      541 aaaacaaaaa ttactcttaa gtcaatctga aatgtcactc ttgaactttt ttgataaact
      601 tgacactgct atttatacct ttaattatcc caatcaatac tggcttttac tttctgacca
      661 gatgtttgac tattattatc caaatatcct atcaaagttt gaatgcgaaa agggtctcta
      721 taaagttggt atcggacaaa aatcatcttt atcactttta aaaagatcat atgaaacgtc
      781 tatcttagcc ctga
//
LOCUS       DATRUK010000144          780 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.146, whole genome shotgun sequence.
ACCESSION   DATRUK010000144 DATRUK010000000
VERSION     DATRUK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.146"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..293
                     /locus_tag="VBE34_001983"
     CDS             <1..293
                     /locus_tag="VBE34_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEO7264639.1"
                     /translation="FTALLLALLLEDSNNKSWISEEDQIKIMNQAFSWLIDELDFRGF
                     DEELGWIHVFAHGADLLTEIVKMKCFSKDDSSKILTIIKRVMITDDILTLNG"
     gene            472..>780
                     /locus_tag="VBE34_001984"
     CDS             472..>780
                     /locus_tag="VBE34_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HEO7264640.1"
                     /translation="MRDEQEIYNLVLNIANQDKRIEAVLLNGSRANPNVPKDDFQDYD
                     IVFVTNFIEDIISDTNYHKKFGDILIMQKPNEFRNKTEYNCFAYLMQFQDLTRIDLRL
                     I"
BASE COUNT          293 a           87 c          145 g          255 t
ORIGIN      
        1 cttttactgc tctattacta gctttattgt tggaggactc aaataataaa agttggatat
       61 ctgaagaaga tcagataaaa attatgaatc aggcattctc ctggcttata gatgaacttg
      121 attttagagg ctttgacgaa gagcttggat ggatacatgt ctttgctcat ggagctgatt
      181 tattaacaga aatagtcaag atgaaatgtt tttcaaaaga tgacagttca aaaatcttaa
      241 ctatcataaa aagagtgatg ataactgatg acatactaac attaaacggt tgaaaatatt
      301 ttgaatcata atcagttgtt cagaaaaatt taaaattgaa tccttggagg tcgtaaaggt
      361 atttccatgc taatttaagt ttatgattgc atgcttagag aaaaatacaa gggtaatata
      421 aaggtattag aaaaaatatg gtaatataaa gtgaagtagg aggtagccat tatgagagat
      481 gagcaagaaa tatataattt ggttttaaac atagcaaatc aagataagag aatcgaagct
      541 gtactattga atggatctcg ggctaaccca aatgtaccga aagatgattt tcaagattat
      601 gatattgttt ttgttactaa ttttatagaa gatattattt ctgatactaa ttatcataaa
      661 aaattcggtg atattcttat aatgcagaaa ccaaatgagt ttagaaataa gacggaatac
      721 aattgctttg cttacttaat gcagtttcaa gatctgacga ggattgattt aagacttatt
//
LOCUS       DATRUK010000145          771 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.147, whole genome shotgun sequence.
ACCESSION   DATRUK010000145 DATRUK010000000
VERSION     DATRUK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.147"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            12..>771
                     /locus_tag="VBE34_001985"
     CDS             12..>771
                     /locus_tag="VBE34_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEO7264641.1"
                     /translation="MKIITFAAIKGGVGKTTLAYNYAEWLAEQNYQVLLIDLDHQCNL
                     TQTYDVFESENTVANIFKRKGDVNIHHLKDNIDLIAGYIRLDSLEKELETKNYKDMLL
                     YMWLEDNYESKNLGKYDFILIDCHPDFSTATRNAVVVSHAVLSPVVPSKFGYSATFNI
                     KERLEEFKAETFDFKTRKSYVTADLYFLGNMIKHNTNSSRELVKQLQDNDNVLSTIPY
                     KELFNRSTLDKISLSEMKQDKALYQKHRKFFDSID"
BASE COUNT          289 a          106 c          123 g          253 t
ORIGIN      
        1 ggagaaataa catgaaaatc attacatttg ctgctattaa aggtggtgta ggaaaaacaa
       61 cattagcata taattatgct gaatggttag ccgaacaaaa ctatcaagtg cttttgattg
      121 atttagatca tcaatgtaat ctaactcaaa cgtatgatgt ctttgaaagt gagaatacag
      181 tcgctaatat ctttaaacga aaaggagacg ttaatattca tcatcttaaa gataacattg
      241 atttaatagc aggttatatt cgattagata gtctagaaaa agagcttgaa accaaaaatt
      301 ataaagatat gctattgtac atgtggttag aagacaatta tgaatctaag aatcttggca
      361 aatatgattt tattttgatt gattgtcatc ccgatttttc aacagcaaca cgtaacgctg
      421 ttgtagtaag tcatgctgtt ttaagtcctg ttgttccgtc aaaatttggg tattctgcaa
      481 cattcaatat taaagaacgt ctagaagaat ttaaagcaga aacatttgat tttaaaactc
      541 gtaaaagcta tgttacagct gatttatatt tcttaggtaa tatgataaag cataacacta
      601 attcttcgag agaacttgta aagcaacttc aagataacga taatgtttta tcaactatcc
      661 cttataaaga attattcaat cgttcaactt tagataagat ttctctttca gaaatgaaac
      721 aagataaagc actttatcaa aagcacagaa aattctttga tagtattgac t
//
LOCUS       DATRUK010000146          700 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.148, whole genome shotgun sequence.
ACCESSION   DATRUK010000146 DATRUK010000000
VERSION     DATRUK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.148"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..684)
                     /locus_tag="VBE34_001986"
     CDS             complement(<1..684)
                     /locus_tag="VBE34_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-like surface protein"
                     /protein_id="HEO7264642.1"
                     /translation="MFRRSKNNSYDTLQTKQRFSIKKFKFGAASVLIGISFLGGFTQG
                     QFNISTDTVFAAEVISGSAVTLNTNMTKNVQNGRAYIDLYDVKNGKIDPLQLITLNSP
                     DLKAQYVIRQGGNYFTQPSELTTVGAASINYTVLKTDGSPHTKPDGQVDIINVSLTIY
                     NSSALRDKIDEVKKKAEDPKWDEGSRDKVLISLDDIKTDIDNNPKTQSDIANKITEVT
                     NLEKILVPRI"
BASE COUNT          214 a          134 c           91 g          261 t
ORIGIN      
        1 gattcgaggt actagtattt tttctaaatt agtaacttca gttattttat tggcaatgtc
       61 tgattgcgtc ttaggattat tatcaatatc tgttttgata tcatctaaac ttatcaaaac
      121 tttatcgcga cttccctcgt cccatttagg gtcttccgct ttctttttaa cttcatctat
      181 tttatctctc aaagctgaag aattgtaaat agtcaatgaa acgtttataa tatccacttg
      241 tccatcaggc ttcgtatgag gacttccatc tgtcttcaat actgtataat taatactagc
      301 tgcaccaaca gtagtcaatt cagaaggttg tgtgaaataa ttgccgcctt gcctaatgac
      361 atactgagct tttaaatcag gtgaatttaa cgtaattaat tgtaatggat ctattttccc
      421 atttttcaca tcatataaat ctatatatgc tctaccattc tgaacatttt tagtcatatt
      481 tgtgtttaac gtaacagcac ttcctgaaat tacttcagct gcaaacacag tatctgtaga
      541 aatattaaat tgcccttgag taaaacctcc taaaaaacta ataccaatta gtacagaagc
      601 tgcaccaaac ttaaacttct taattgaaaa ccgttgtttc gtctgtaaag tatcataact
      661 gttattttta gaccttctaa acataatatt ctcctttact
//
LOCUS       DATRUK010000147          680 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.150, whole genome shotgun sequence.
ACCESSION   DATRUK010000147 DATRUK010000000
VERSION     DATRUK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.150"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(78..151)
                     /locus_tag="VBE34_001987"
     tRNA            complement(78..151)
                     /locus_tag="VBE34_001987"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Arg,seq:acg)
     gene            complement(163..247)
                     /locus_tag="VBE34_001988"
     tRNA            complement(163..247)
                     /locus_tag="VBE34_001988"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Leu,seq:taa)
     gene            complement(256..327)
                     /locus_tag="VBE34_001989"
     tRNA            complement(256..327)
                     /locus_tag="VBE34_001989"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(293..295),aa:Gly,seq:gcc)
     gene            complement(361..433)
                     /locus_tag="VBE34_001990"
     tRNA            complement(361..433)
                     /locus_tag="VBE34_001990"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(398..400),aa:Thr,seq:tgt)
     gene            complement(444..525)
                     /locus_tag="VBE34_001991"
     tRNA            complement(444..525)
                     /locus_tag="VBE34_001991"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(489..491),aa:Leu,seq:tag)
     gene            complement(535..607)
                     /locus_tag="VBE34_001992"
     tRNA            complement(535..607)
                     /locus_tag="VBE34_001992"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(572..574),aa:Lys,seq:ttt)
     gene            complement(610..680)
                     /locus_tag="VBE34_001993"
     tRNA            complement(610..680)
                     /locus_tag="VBE34_001993"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(647..649),aa:Asp,seq:gtc)
BASE COUNT          183 a          199 c          150 g          148 t
ORIGIN      
        1 gaagacagga ttcgaacctg cgacaccttg gtcccaaacc aagtactcta ccaagctgag
       61 ctacttcccg aaaaatatgc accctagagg agtcgaacct ctaaccgcct gattcgtagt
      121 caggtactct atccagttga gctaagggtg ctaaatatta tatgccgagg accggaatcg
      181 aaccggtacg atgtttacca tcgcaggatt ttaagtcctg tgcgtctgcc agttccgcca
      241 ccccggcctc taacaagcga acgacggggt tcgaacccgc gaccctcacc ttggcaaggt
      301 gatgttctac cactgaacta cgttcgcact aaagacacta tttatcctat aaaattgtaa
      361 tgccggctac atgacttgaa cacgcgaccc tctgattaca aatcagatgc tctaccaact
      421 gagctaagcc ggcaatctac taatgcgggt gaagggactt gaacccccac gccgttaagc
      481 gccagatcct aaatctggtg cgtctgccaa ttccgccaca cccgcatttc taaatgaccc
      541 gtactgggct cgaaccagtg acccattgat taaaagtcaa ttgctctacc aactgagcta
      601 acgagtctac ggtcccgacg ggaatcgaac ccgcgatctt cgccgtgaca gggcgacgtg
      661 ataaccgcta cactacggga
//
LOCUS       DATRUK010000148          668 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.151, whole genome shotgun sequence.
ACCESSION   DATRUK010000148 DATRUK010000000
VERSION     DATRUK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.151"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(<1..569)
                     /locus_tag="VBE34_001994"
     CDS             complement(<1..569)
                     /locus_tag="VBE34_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEO7264643.1"
                     /translation="MNSLDNLLSHNLEDNRHLLPYEHMHTEVRNGYPDILFHWHPELE
                     ISYVHEGTARYHIDYDFFNSQSGDIILIRPNGMHSIHPIENKEHITDSIKFHLDLIGY
                     SIVDQVSLRYLQPLQTSSFKFIQCIKPSMTGYNDIKNCLFDIFNISKEENRHFELLLK
                     AKLNELLYLLYYHQYVIKKHTDDTYRKNE"
BASE COUNT          227 a           90 c          115 g          236 t
ORIGIN      
        1 ctctcgtttt ttctgtaggt atcatcagta tgttttttaa tgacatattg atgataatag
       61 agtaaataca agagttcgtt taatttagct tttaggagta gctcaaaatg tcgattttct
      121 tcttttgaaa tattaaaaat atcaaacaaa caatttttga tatcattata tcctgtcata
      181 ctaggtttga tacattgaat aaacttaaaa gagctagtct gcaacggttg taaataacga
      241 aggctaactt ggtctacaat agaatatcct attaaatcta agtgaaattt aatgctatct
      301 gtgatatgct ctttattttc aatcggatga atagaatgca taccatttgg acggatcaag
      361 ataatatcac cagattggct attaaaaaaa tcataatcga tgtgataacg tgcggtcccc
      421 tcgtggacat atgaaatttc aagttccgga tgccagtgaa ataaaatgtc agggtaacca
      481 tttctaacct cagtatgcat gtgttcatag ggtaataaat gccggttatc ttctaaatta
      541 tgtgatagta aattatcaag tgagttcatc gtttttcttt ctaaaatcgt aggatagtat
      601 tattataaca gaaaactatc aaaaaatggg tttcgtagga tagtattatt ttttgaaagg
      661 atagtgta
//
LOCUS       DATRUK010000149          639 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.152, whole genome shotgun sequence.
ACCESSION   DATRUK010000149 DATRUK010000000
VERSION     DATRUK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.152"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            250..>639
                     /locus_tag="VBE34_001995"
     CDS             250..>639
                     /locus_tag="VBE34_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7264644.1"
                     /translation="MKEKVTVIIPIYNSEAYLKECVQSVLQQTHPLIEVILIDDGSTD
                     NSGEICDNLSQEDNRILVFHKKNGGVSSARNLGLDKSTGEFITFVDSDDFVAPNMIEI
                     MLKNLITENADIAEVDFDISNERDYRKK"
BASE COUNT          228 a           81 c          114 g          216 t
ORIGIN      
        1 tgaaggaata ttcctaggat cgcattctac ttatattagt gtcttttaca ggacttcttt
       61 attaggaatt gttctttatt tttctgcctt tatactttta tataaagaag cgatttcaaa
      121 aaattataaa atctacagat tattttttta tacgttatta tgttacacgc tctttgagga
      181 aatagatcct aatcattgga gtattgtatt attattctca acttttggta tagtgggaag
      241 ggctaaaaaa tgaaagaaaa agtaacagtc attataccta tatacaactc agaagcatac
      301 cttaaagaat gtgtgcaatc cgtactacaa cagactcatc cattgataga agttatacta
      361 attgatgatg gatccactga taatagtgga gaaatttgtg ataatttatc tcaggaagat
      421 aatcgcatac ttgtatttca taaaaaaaat ggaggggtct cttcggcaag gaacctaggt
      481 ctagataaat ccacaggaga attcataaca tttgtggata gtgatgattt tgtagcaccg
      541 aatatgattg aaataatgtt aaaaaattta atcactgaga atgctgatat agcagaagta
      601 gattttgata tttcgaatga gagagattat agaaagaag
//
LOCUS       DATRUK010000150          633 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.153, whole genome shotgun sequence.
ACCESSION   DATRUK010000150 DATRUK010000000
VERSION     DATRUK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.153"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
BASE COUNT          226 a           74 c          105 g          228 t
ORIGIN      
        1 attttgtcac tggtgtctag taacttatag attgataatt ttactagtaa acgtaattct
       61 tcgctttaag agttaaatgt ctatttattg taagctaaat tgggaggtga acttatgtaa
      121 aattagatag gtactgtcaa gtacgggatg attattgaaa cagccagtat gcatcataaa
      181 atctgtattg cttaataact atttccttaa ccagacatca gttcattgtt tatcatcgct
      241 accctaagtc tagttttttc aatagagcat taggtagttt ttgataataa aactatataa
      301 acatgagaat tagatttcgt atcgcattct tcataatgag ttatttgaga ttttcctttg
      361 aataaataga tacgaaattc agtaacttca tatataaacg gctctatcat tgagatagtt
      421 tgtcaaatga agaaatttta atggaaatag ttttaaaaac attagttgta ggcgatgtaa
      481 aaatattaat ccagtggatg caatagttgc ggagtaaaaa tagagaggag taattaggaa
      541 gtgataaaaa atgctatagc atatattacc agaaaaaaaa atagaacact tattatattt
      601 gctattttaa caattgttct ttcttgcttg tat
//
LOCUS       DATRUK010000151          620 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.155, whole genome shotgun sequence.
ACCESSION   DATRUK010000151 DATRUK010000000
VERSION     DATRUK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.155"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            34..620
                     /locus_tag="VBE34_001996"
                     /pseudo
     CDS             34..620
                     /locus_tag="VBE34_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_088186184.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS861 family transposase"
BASE COUNT          218 a           95 c          140 g          167 t
ORIGIN      
        1 gacaaaaaat ctaacgattg gggtgctttt ctgatgaaat taagttatga agataaacta
       61 gaaatatacg agttaagaaa gatcgggatg tcctggtctc agattagtca acgatatgat
      121 gttcgtatct cgaatcttaa atacatgata aaactcatgg atcggtatgg tgtagaaatc
      181 gtcgaaaaag gtagaaatga gtattatcca cctgaattaa agcaggaaat gatagataaa
      241 gtcttgattc atggttgttc tcaactctct gtttctcttg attatgctct ctctaattgt
      301 tctatactca caaattggct ttcccaattt aagaaaaatg gttatactat tgttgagaaa
      361 acaagaggga gaccgagtaa aatgggacgt aagcgtaaga aaacttggga agaaatgaca
      421 gagctcgagc gacttcagga agaaaatgag cgcttgagaa cggagaatgc ctttctaaaa
      481 aagttgaggg atttgcgctt gagggacgaa gccttacaga gcgaacggca gaaacaatta
      541 gagaaatggt cacaggagga ttccgactag acctcttact tgagataact aagatagctc
      601 gtgcaactta ctattatcaa
//
LOCUS       DATRUK010000152          618 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.156, whole genome shotgun sequence.
ACCESSION   DATRUK010000152 DATRUK010000000
VERSION     DATRUK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.156"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..>618
                     /locus_tag="VBE34_001997"
     CDS             <1..>618
                     /locus_tag="VBE34_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO7264645.1"
                     /translation="DKEKLDYWKTIITFLHNVLGDNYEIVLHVVDENDIYIGELVNSH
                     ISGRTISSPLTTFALDLIKNKVYKEKDFVTNYKAIVSPLNKEVRGSTFFIKNAQNELE
                     GMLCINLDISAYQNIALDILDLVNLNVNKILSKSPQKISLPQQEEPVEVLSGNIQDII
                     SEIVDPSLLNQNIHLSQEVKVEIVSKLHEKGVFQLKGAVSKVAEVL"
BASE COUNT          213 a           93 c          107 g          205 t
ORIGIN      
        1 gataaagaaa aattagacta ttggaagacg ataataacgt ttctccataa tgttttaggt
       61 gataattacg aaattgtttt gcatgtagta gatgaaaatg atatctatat tggtgagttg
      121 gttaatagtc acattagtgg acgaaccata agttcacctc taacgacttt cgcccttgat
      181 ttaatcaaga ataaagttta taaggaaaaa gattttgtta ctaactataa ggcgattgtc
      241 agccctctca ataaagaagt tcgtggatct actttcttca tcaagaatgc tcaaaatgaa
      301 cttgaaggga tgttatgtat caaccttgat atttctgcat accaaaatat tgctttggat
      361 attctagact tagttaattt aaatgttaac aaaatactat ctaagtcccc tcaaaaaatt
      421 tctctccctc aacaggagga acctgtagaa gttctatcag gcaatattca ggatattatt
      481 agtgagattg ttgatcctag tcttttaaat cagaatattc atttaagtca agaagttaag
      541 gtagaaattg tttctaaact tcacgaaaaa ggtgtattcc aattaaaagg tgccgtttca
      601 aaggtcgctg aagttctc
//
LOCUS       DATRUK010000153          612 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.157, whole genome shotgun sequence.
ACCESSION   DATRUK010000153 DATRUK010000000
VERSION     DATRUK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.157"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..>612
                     /locus_tag="VBE34_001998"
     CDS             <1..>612
                     /locus_tag="VBE34_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HEO7264646.1"
                     /translation="DIIQLPNNSLQNFQLKPVGKGSYATVFCYFDENYNKTFALKRAS
                     KIISSKDLERFYLEFDTMKKLHSPYILEVYSIDKTKNEYIMEYANTTLLKYITTHNQK
                     LTFDERKSICHQIIKGFEYLSEKGILHRDISPNNILIKEYEDVKIIKIADLGNVKLSD
                     SILTSLDTEIRGAFNDYSGLQRVGYSNYNFYFEGFALSKLLYFV"
BASE COUNT          228 a           69 c          103 g          212 t
ORIGIN      
        1 tgatataatc cagttaccta ataattcatt acagaatttt caattgaagc cagttggtaa
       61 aggttcttat gctactgtct tttgttattt tgatgagaat tacaacaaaa cttttgcatt
      121 aaaacgtgcg agtaaaataa tttcatcgaa agatttagag agattttatt tagaatttga
      181 cacgatgaaa aagctacact ctccatatat actggaagtt tattctattg ataaaacaaa
      241 aaatgagtat attatggagt atgcgaacac gacccttttg aaatacatca caactcataa
      301 tcaaaaattg acatttgatg agaggaagtc tatatgccat cagattataa aaggatttga
      361 atatttatct gagaaaggaa tacttcatag agatatctct ccaaataata ttttgataaa
      421 agaatatgag gatgtaaaga taattaaaat tgcagattta gggaatgtaa agttgagtga
      481 tagtatatta acgtctttag atacagaaat aagaggtgcg tttaatgatt attccggact
      541 tcaacgagta ggatatagta actataattt ttattttgaa ggttttgctt tgtcaaaatt
      601 attgtatttt gt
//
LOCUS       DATRUK010000154          536 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.160, whole genome shotgun sequence.
ACCESSION   DATRUK010000154 DATRUK010000000
VERSION     DATRUK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.160"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..486
                     /locus_tag="VBE34_001999"
     CDS             <1..486
                     /locus_tag="VBE34_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7264647.1"
                     /translation="KKNKTNPSGATSAKVVKHKKTDEERAEDYIKTLGLNPSDYIKEM
                     DYSKVDFRGDLSSVSGNYNNFTVENINQTFEKGDKSLKDIIEIFGKPDTIWVNYVDNQ
                     YNTDLYWKKDDTDDSNYIHAEWSEPKDEYDVQSLSGTASNSGTGKFQSFIGLDGFEPA
                     Q"
BASE COUNT          206 a           63 c          104 g          163 t
ORIGIN      
        1 aaaaagaata agacaaatcc tagtggtgcg acaagtgcaa aagtcgttaa acacaaaaaa
       61 actgatgaag aacgagcaga agattatata aaaacgttag gattaaaccc gagtgattat
      121 ataaaagaaa tggattattc aaaggttgat tttagggggg atttatcatc agtatcaggt
      181 aattataata attttactgt tgaaaacatc aatcaaactt ttgaaaaagg tgataaatct
      241 ttaaaagata ttattgaaat ttttggaaag cctgatacaa tttgggttaa ttacgttgac
      301 aatcagtaca atactgattt gtactggaaa aaagatgaca cagatgattc taattatatt
      361 catgcagaat ggtctgagcc aaaagatgaa tatgatgttc aaagtttaag tggcacggct
      421 tcaaattcag gtactggcaa gtttcagtca ttcattggat tagatggatt tgagcctgca
      481 cagtaggata aatagaaaga tcacgttatt aaatgtggtc ttttacttta actttt
//
LOCUS       DATRUK010000155          522 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.161, whole genome shotgun sequence.
ACCESSION   DATRUK010000155 DATRUK010000000
VERSION     DATRUK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.161"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            complement(27..518)
                     /locus_tag="VBE34_002000"
     CDS             complement(27..518)
                     /locus_tag="VBE34_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO7264648.1"
                     /translation="MLSFQIVEKPAMILAGVTLENVKSNQECPKLWEKLHKQYNPSKL
                     KEKGIQQAIGICKTQPDFRFDYSATYQVETSVQAPKGLEIIRIPSATYAVISVKGPMP
                     SSLQETWRKIIQGFFQENNLKPANSPNLEIYSSQHPQDTDYQMEIWLAIADITNNSKT
                     NLI"
BASE COUNT          145 a           77 c           98 g          202 t
ORIGIN      
        1 aaggggcttt tttatattaa gcaatactag attaagtttg ttttcgagtt attagtaata
       61 tctgcgatag caagccaaat ttccatttga taatcagtat cttgtggatg ttgagatgaa
      121 taaatttcta agtttggcga gttagctggt tttagattat tctcttgaaa aaatccttgt
      181 attatttttc gccatgtttc ttgaagactc gatggcattg gacctttaac agaaatgact
      241 gcataggtag cgcttgggat cctaataatt tctaaccctt ttggggcttg gacacttgtc
      301 tctacttgat aagtggcact gtaatcgaaa cgaaaatcag gttgtgtttt acaaatgcca
      361 atggcttgct ggataccttt ttcttttagt tttgatggat tatattgttt atgtaatttt
      421 tcccaaagtt taggacattc ttgatttgac tttacatttt ctaaagttac tccagcaagt
      481 atcattgctg gcttttcaac aatttgaaat gatagcataa aa
//
LOCUS       DATRUK010000156          516 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.163, whole genome shotgun sequence.
ACCESSION   DATRUK010000156 DATRUK010000000
VERSION     DATRUK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.163"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..>516
                     /locus_tag="VBE34_002001"
     CDS             <1..>516
                     /locus_tag="VBE34_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSag9 family transposase"
                     /protein_id="HEO7264649.1"
                     /translation="EKLLVESREGIPLNKETFWKIDRVLSNGVKKGQRIYHILKTNDL
                     EVSSSTVYRHIKKGYLSITPIDLPRAVKFKKRRKSTLPPIPKAIKEGRRYEDFIEHMN
                     QSELNSWLEMDTVIGRIGGKVLLTFNVAFCNFIFAKLMDSKTAIETAKHIQVIKRTLY
                     DNKRDFFELFPV"
BASE COUNT          172 a          115 c          102 g          127 t
ORIGIN      
        1 gaaaaacttt tagttgaatc tagagaagga atccctttga acaaagagac cttctggaaa
       61 atagatagag tgctttctaa tggggtcaag aagggccaac gtatctatca tatcctcaaa
      121 accaacgatc tagaagtgag ttcttcaacc gtttatcgac acatcaagaa aggctaccta
      181 tccatcacac caatcgacct acccagagct gtgaagttca aaaaaaggcg aaagagcaca
      241 ctccctccta ttccaaaagc gattaaagaa gggcgacggt acgaggattt tatagaacac
      301 atgaaccaat cagagctgaa ctcctggctt gaaatggaca cggttattgg acggattggt
      361 ggaaaggtcc ttctcacctt caatgtcgcc ttctgtaact tcatctttgc caaactgatg
      421 gattctaaga cagctatcga aactgctaaa cacatacagg tcattaagag gacactctat
      481 gataacaaga gagacttctt cgaactcttt cctgtt
//
LOCUS       DATRUK010000157          502 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.165, whole genome shotgun sequence.
ACCESSION   DATRUK010000157 DATRUK010000000
VERSION     DATRUK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.165"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..>502
                     /locus_tag="VBE34_002002"
     CDS             <1..>502
                     /locus_tag="VBE34_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0236 family protein"
                     /protein_id="HEO7264650.1"
                     /translation="QMRTKGYKRINQSERTVVFSFGEITFSRSRWTNGFETRIPVDEW
                     LGLEKYKRYSIEFLYHVAKLATMMPYRQVCKVIDSTLQTIITKDCVLKAVKFVEKLLK
                     EKERYRFYLEEPPERKKVKKLYVEGDGVMIKSTDSREERRYLDLTHFVIHTGSKKVST
                     KRYELRD"
BASE COUNT          193 a           62 c          104 g          143 t
ORIGIN      
        1 tcaaatgaga acgaaaggct ataagaggat caatcagtct gaacgtacgg ttgttttctc
       61 ttttggagaa ataacattta gtaggagtcg ctggacaaat ggctttgaaa ctagaatacc
      121 agtagatgag tggttaggtc ttgaaaaata taagagatat tcaatagaat tcttatatca
      181 tgttgcaaaa ttggctacaa tgatgcctta tcgtcaagtt tgcaaagtaa tagatagcac
      241 tttgcaaaca atcataacaa aagactgtgt tttaaaagca gtaaaatttg tagaaaaatt
      301 gttaaaagaa aaagaacgct atcgttttta tttggaagag ccacccgaac gtaaaaaagt
      361 gaaaaaactg tatgttgagg gtgatggagt catgattaaa agcacagatt ctagagagga
      421 aagaaggtat ttagatttaa cacattttgt tattcataca ggctcaaaaa aagtttctac
      481 taaaagatat gaattgcggg ac
//
LOCUS       DATRUK010000158          482 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.166, whole genome shotgun sequence.
ACCESSION   DATRUK010000158 DATRUK010000000
VERSION     DATRUK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.166"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            98..>482
                     /locus_tag="VBE34_002003"
     rRNA            98..>482
                     /locus_tag="VBE34_002003"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          148 a           93 c          135 g          106 t
ORIGIN      
        1 gacgaacgaa tgtgcagggt ggatttacat tgtaaatccg tcaatgacaa aaaacaataa
       61 atctgtcagc gagacagaaa agagcaaagc tcaaactttt aatgagagtt tgatcctggc
      121 tcaggacgaa cgctggcggc gtgcctaata catgcaagta gaacgctgag gtttggtgtt
      181 tacactagac tgatgagttg cgaacgggtg agtaacgcgt aggtaacctg cctcatagcg
      241 ggggataact attggaaacg atagctaata ccgcataaga gtaattaaca catgttagtt
      301 atttaaaagg agcaattgct tcactgtgag atggacctgc gttgtattag ctagttggtg
      361 aggtaaaggc tcaccaaggc gacgatacat agccgacctg agagggtgat cggccacact
      421 gggactgaga cacggcccag actcctacgg gaggcagcag tagggaatct tcggcaatgg
      481 ac
//
LOCUS       DATRUK010000159          429 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.denovo.167, whole genome shotgun sequence.
ACCESSION   DATRUK010000159 DATRUK010000000
VERSION     DATRUK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.denovo.167"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            <1..118
                     /locus_tag="VBE34_002004"
     CDS             <1..118
                     /locus_tag="VBE34_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001920755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7264651.1"
                     /translation="KGNKLPATGENATPFFNVVALTIMSSVGLLSVSKKKED"
BASE COUNT          141 a           65 c           64 g          159 t
ORIGIN      
        1 taaaggaaat aaactaccag caacaggtga gaatgcaact ccattcttta atgttgtagc
       61 tttgacaatt atgtcatcag ttggtttatt atctgtttct aagaaaaaag aggattaatc
      121 ttttgaccta aaatgtcact aaacttttca ccatttattg gtgtgaacac attaataaag
      181 ttatgtatct ctcttcaaca aacttaatta aagtgtttca atttttcgag tttaattctt
      241 gaaaaatcct atcgagatta ttaatttcga taggattttg attttgtgta agcgtccaat
      301 aacgaggtat cttccatttt cttccctatt ccgtaagcat ccaatatacc ttgttattgg
      361 acgcttacta taaaactgaa gaaatgatcc tatttagttg aaatttgtaa atgaagtgta
      421 tcattagag
//
LOCUS       DATRUK010000160         2311 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699539-rid18549093.guided.114, whole genome shotgun sequence.
ACCESSION   DATRUK010000160 DATRUK010000000
VERSION     DATRUK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699539
            Sequence Read Archive: SRR7788285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789802.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789802.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:10:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,006
            CDSs (total)                      :: 1,961
            Genes (coding)                    :: 1,891
            CDSs (with protein)               :: 1,891
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 49 of 70
            Pseudo Genes (incomplete)         :: 8 of 70
            Pseudo Genes (internal stop)      :: 22 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699539-rid18549093.guided.114"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2010-03-19"
                     /collected_by="RMPRU"
     gene            77..1996
                     /gene="tet(M)"
                     /locus_tag="VBE34_002005"
     CDS             77..1996
                     /gene="tet(M)"
                     /locus_tag="VBE34_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO7264652.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTD
                     NTLLERQRGITIQTAITSFQWKNTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKIGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     HPNMRVMNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSILGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTSFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2115..2282
                     /locus_tag="VBE34_002006"
     CDS             2115..2282
                     /locus_tag="VBE34_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO7264653.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
BASE COUNT          802 a          355 c          472 g          682 t
ORIGIN      
        1 taaaaatccc agtgataaga gtatttatca ctgggatttt tatgcccttt tgggcttttg
       61 aatggaggaa aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcggg
      121 aaaaactacc ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag
      181 cgtggacaga ggtacaacga aaacggataa tacgctttta gaacgtcaga gaggaattac
      241 aattcagacg gcgataacct cttttcagtg gaaaaatact aaggtgaaca tcatagacac
      301 gccaggacat atggattttt tagcagaagt atatcgttca ttatcagtat tagatggggc
      361 aattctactg atttctgcaa aagatggcgt acaagcacaa actcgtatat tgtttcatgc
      421 acttaggaaa ataggtattc ccacaatctt ttttatcaat aagattgacc aaaatggaat
      481 tgatttatca acggtttatc aggatattaa agagaaactt tctgcggaaa ttgtaatcaa
      541 acagaaggta gaactgcatc ctaatatgcg tgtaatgaac tttaccgaat ctgaacaatg
      601 ggatatggta atagaaggaa atgattacct tttggagaaa tatacgtctg ggaaattatt
      661 ggaagcatta gaactcgaac aagaggaaag cataagattt cataattgtt ccctgttccc
      721 tgtttatcac ggaagtgcaa aaaacaatat agggattgat aaccttatag aagtgattac
      781 gaataaattt tattcatcaa cacatcgagg tcagtctgaa ctttgcggaa aagttttcaa
      841 aattgagtat tcggaaaaaa gacagcgtct tgcatatata cgtctttata gtggcgtact
      901 gcatttgcga gattcggtta gaatatcgga aaaggaaaaa ataaaaatta cagaaatgta
      961 tacttcaata aatggtgaat tatgtaaaat cgataaggct tattccgggg aaattgttat
     1021 tttgcagaat gagtttttga agttaaatag tattcttgga gatacaaagc tattgccaca
     1081 gagagagaga attgaaaatc ccctccctct gctgcaaacg actgttgaac cgagcaaacc
     1141 tcaacaaagg gaaatgttac ttgatgcact tttagaaatc tccgacagtg acccgcttct
     1201 gcgatattat gtggattctg cgacacatga aatcatactt tctttcttag ggaaagtaca
     1261 aatggaagtg acttgtgctc tgctgcaaga aaagtatcat gtggagatag aaataaaaga
     1321 gcctacagtc atttatatgg aaagaccgtt aaaaaaagca gagtatacca ttcacatcga
     1381 agttccaccg aatcctttct gggcttccat tggtctatct gtagcacagc ttccattagg
     1441 gagcggagta cagtatgaga gctcggtttc tcttggatac ttaaatcaat cgtttcaaaa
     1501 tgcagttatg gaggggatac gctatggctg tgaacaagga ttgtatggtt ggaatgtgac
     1561 ggactgtaaa atctgtttta agtatggctt atactatagc cctgttagta ccccagcaga
     1621 ttttcggatg cttgctccta ttgtattgga acaagtctta aaaaaagctg gaacagaatt
     1681 gttagagcca tatcttagtt ttaaaattta tgcgccacag gaatatcttt cacgagcata
     1741 caacgatgct cctaaatatt gtgcgaacat cgtagacact caattgaaaa ataatgaggt
     1801 cattcttagt ggagaaatcc ctgctcggtg tattcaagaa tatcgtagtg atttaacttc
     1861 ctttacaaat ggacgtagtg tttgtttaac agagttaaaa gggtaccatg ttactaccgg
     1921 tgaacctgtt tgccagcccc gtcgtccaaa tagtcggata gataaagtac gatatatgtt
     1981 caataaaata acttagtgta ttttatgttg ttatataaat atggtttctt gttaaataag
     2041 atgaaatatt ttttaataaa gatttgaatt aaagtgtaaa ggaggagata gttattataa
     2101 actacaagtg gatattgtgt cctgtatgtg gaaataaaac acgattaaag ataagggaag
     2161 atactgaatt aaaaaaattc cccctctatt gtccgaaatg cagacaagaa aatttaattg
     2221 aaataaagca gttcaaagta actgtgatta cagagccaga cgcaaagacg cagagccgat
     2281 aaaatgagat taatacaatc tcattttatc g
//
